AGRN cDNA ORF clone, Gallus gallus(chicken)

The following AGRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa147020 XM_025142490.1
Latest version!
Gallus gallus agrin (AGRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa45017 XM_015296844.1
Latest version!
Gallus gallus agrin (AGRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa45012 XM_015296839.1
Latest version!
Gallus gallus agrin (AGRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa45013 XM_015296840.1
Latest version!
Gallus gallus agrin (AGRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa45014 XM_015296841.1
Latest version!
Gallus gallus agrin (AGRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa45015 XM_015296842.2
Latest version!
Gallus gallus agrin (AGRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa45016 XM_015296843.2
Latest version!
Gallus gallus agrin (AGRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa45018 XM_015296845.2
Latest version!
Gallus gallus agrin (AGRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa28431 NM_205527.3
Latest version!
Gallus gallus agrin (AGRN), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OGa45018 XM_015296845.1 Gallus gallus agrin (AGRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa45015 XM_015296842.1 Gallus gallus agrin (AGRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa45016 XM_015296843.1 Gallus gallus agrin (AGRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa147020
    Clone ID Related Accession (Same CDS sequence) XM_025142490.1
    Accession Version XM_025142490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product agrin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGACGGCTT GCCAGTACCC CATGGCACCG GGGGCCTTGG AGCGGGACCG GCTGTACCAG 
    CACAAGGTCT CCTTGGTGGT GCGCTACTTC ATGATCCCGT GCAACATTTG CCTCATCCTC
    CTGGCCACCT CCACGCTGGG ATTTGCTGTG CTGCTCTTCC TCAATAACTA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCGAGAAGGC TTTGCAATCC AACCACTTTG AGCTGAGCAT CAAAACAGAA
    GCAACCCAGG GCCTGATCTT GTGGAGTGGG AAGGGGCTGG AGCGATCCGA CTACATCGCC
    CTCGCCATCG TGGATGGCTT CGTGCAGATG ATGTATGACC TGGGCTCCAA GCCAGTCGTC
    CTGCGGTCCA CAGTCCCCAT CAACACCAAC CACTGGACCC ACATCAAAGC ATACAGAGTA
    CAGAGAGAAG GCTCCCTCCA GGTCGGCAAC GAAGCTCCCA TCACTGGCTC TTCACCGCTG
    GGTGCCACAC AGCTGGACAC GGATGGGGCC CTGTGGCTGG GGGGAATGGA GCGGCTCAGC
    GTGGCTCACA AGCTGCCCAA GGCTTACAGC ACTGGCTTTA TTGGCTGTAT AAGGGACGTG
    ATTGTCGACC GCCAAGAACT GCATCTGGTG GAGGACGCTT TAAACAACCC CACGATATTA
    CACTGCTCAG CCAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024998258.1
    CDS176..6073
    Translation

    Target ORF information:

    RefSeq Version XM_025142490.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025142490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGACGGCTT GCCAGTACCC CATGGCACCG GGGGCCTTGG AGCGGGACCG GCTGTACCAG 
    CACAAGGTCT CCTTGGTGGT GCGCTACTTC ATGATCCCGT GCAACATTTG CCTCATCCTC
    CTGGCCACCT CCACGCTGGG ATTTGCTGTG CTGCTCTTCC TCAATAACTA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCGAGAAGGC TTTGCAATCC AACCACTTTG AGCTGAGCAT CAAAACAGAA
    GCAACCCAGG GCCTGATCTT GTGGAGTGGG AAGGGGCTGG AGCGATCCGA CTACATCGCC
    CTCGCCATCG TGGATGGCTT CGTGCAGATG ATGTATGACC TGGGCTCCAA GCCAGTCGTC
    CTGCGGTCCA CAGTCCCCAT CAACACCAAC CACTGGACCC ACATCAAAGC ATACAGAGTA
    CAGAGAGAAG GCTCCCTCCA GGTCGGCAAC GAAGCTCCCA TCACTGGCTC TTCACCGCTG
    GGTGCCACAC AGCTGGACAC GGATGGGGCC CTGTGGCTGG GGGGAATGGA GCGGCTCAGC
    GTGGCTCACA AGCTGCCCAA GGCTTACAGC ACTGGCTTTA TTGGCTGTAT AAGGGACGTG
    ATTGTCGACC GCCAAGAACT GCATCTGGTG GAGGACGCTT TAAACAACCC CACGATATTA
    CACTGCTCAG CCAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45017
    Clone ID Related Accession (Same CDS sequence) XM_015296844.1
    Accession Version XM_015296844.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product agrin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGACGGCTT GCCAGTACCC CATGGCACCG GGGGCCTTGG AGCGGGACCG GCTGTACCAG 
    CACAAGGTCT CCTTGGTGGT GCGCTACTTC ATGATCCCGT GCAACATTTG CCTCATCCTC
    CTGGCCACCT CCACGCTGGG ATTTGCTGTG CTGCTCTTCC TCAATAACTA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCCACCTGAG CAATGAGATC CCTGCTCCCG ACGCATTGGA CTACCCTGCT
    GAGCCCAGCG AGAAGGCTTT GCAATCCAAC CACTTTGAGC TGAGCATCAA AACAGAAGCA
    ACCCAGGGCC TGATCTTGTG GAGTGGGAAG GGGCTGGAGC GATCCGACTA CATCGCCCTC
    GCCATCGTGG ATGGCTTCGT GCAGATGATG TATGACCTGG GCTCCAAGCC AGTCGTCCTG
    CGGTCCACAG TCCCCATCAA CACCAACCAC TGGACCCACA TCAAAGCATA CAGAGTACAG
    AGAGAAGGCT CCCTCCAGGT CGGCAACGAA GCTCCCATCA CTGGCTCTTC ACCGCTGGGT
    GCCACACAGC TGGACACGGA TGGGGCCCTG TGGCTGGGGG GAATGGAGCG GCTCAGCGTG
    GCTCACAAGC TGCCCAAGGC TTACAGCACT GGCTTTATTG GCTGTATAAG GGACGTGATT
    GTCGACCGCC AAGAACTGCA TCTGGTGGAG GACGCTTTAA ACAACCCCAC GATATTACAC
    TGCTCAGCCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152330.1
    CDS1382..7336
    Translation

    Target ORF information:

    RefSeq Version XM_015296844.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296844.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGACGGCTT GCCAGTACCC CATGGCACCG GGGGCCTTGG AGCGGGACCG GCTGTACCAG 
    CACAAGGTCT CCTTGGTGGT GCGCTACTTC ATGATCCCGT GCAACATTTG CCTCATCCTC
    CTGGCCACCT CCACGCTGGG ATTTGCTGTG CTGCTCTTCC TCAATAACTA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCCACCTGAG CAATGAGATC CCTGCTCCCG ACGCATTGGA CTACCCTGCT
    GAGCCCAGCG AGAAGGCTTT GCAATCCAAC CACTTTGAGC TGAGCATCAA AACAGAAGCA
    ACCCAGGGCC TGATCTTGTG GAGTGGGAAG GGGCTGGAGC GATCCGACTA CATCGCCCTC
    GCCATCGTGG ATGGCTTCGT GCAGATGATG TATGACCTGG GCTCCAAGCC AGTCGTCCTG
    CGGTCCACAG TCCCCATCAA CACCAACCAC TGGACCCACA TCAAAGCATA CAGAGTACAG
    AGAGAAGGCT CCCTCCAGGT CGGCAACGAA GCTCCCATCA CTGGCTCTTC ACCGCTGGGT
    GCCACACAGC TGGACACGGA TGGGGCCCTG TGGCTGGGGG GAATGGAGCG GCTCAGCGTG
    GCTCACAAGC TGCCCAAGGC TTACAGCACT GGCTTTATTG GCTGTATAAG GGACGTGATT
    GTCGACCGCC AAGAACTGCA TCTGGTGGAG GACGCTTTAA ACAACCCCAC GATATTACAC
    TGCTCAGCCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45012
    Clone ID Related Accession (Same CDS sequence) XM_015296839.1
    Accession Version XM_015296839.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product agrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCCACCTGAG CAATGAGATC CCTGCTCCCG ACGCATTGGA CTACCCTGCT
    GAGCCCAGCG AGAAGGCTTT GCAATCCAAC CACTTTGAGC TGAGCATCAA AACAGAAGCA
    ACCCAGGGCC TGATCTTGTG GAGTGGGAAG GGGCTGGAGC GATCCGACTA CATCGCCCTC
    GCCATCGTGG ATGGCTTCGT GCAGATGATG TATGACCTGG GCTCCAAGCC AGTCGTCCTG
    CGGTCCACAG TCCCCATCAA CACCAACCAC TGGACCCACA TCAAAGCATA CAGAGTACAG
    AGAGAAGGCT CCCTCCAGGT CGGCAACGAA GCTCCCATCA CTGGCTCTTC ACCGCTGGGT
    GCCACACAGC TGGACACGGA TGGGGCCCTG TGGCTGGGGG GAATGGAGCG GCTCAGCGTG
    GCTCACAAGC TGCCCAAGGC TTACAGCACT GGCTTTATTG GCTGTATAAG GGACGTGATT
    GTCGACCGCC AAGAACTGCA TCTGGTGGAG GACGCTTTAA ACAACCCCAC GATATTACAC
    TGCTCAGCCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152325.1
    CDS172..6426
    Translation

    Target ORF information:

    RefSeq Version XM_015296839.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296839.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCCACCTGAG CAATGAGATC CCTGCTCCCG ACGCATTGGA CTACCCTGCT
    GAGCCCAGCG AGAAGGCTTT GCAATCCAAC CACTTTGAGC TGAGCATCAA AACAGAAGCA
    ACCCAGGGCC TGATCTTGTG GAGTGGGAAG GGGCTGGAGC GATCCGACTA CATCGCCCTC
    GCCATCGTGG ATGGCTTCGT GCAGATGATG TATGACCTGG GCTCCAAGCC AGTCGTCCTG
    CGGTCCACAG TCCCCATCAA CACCAACCAC TGGACCCACA TCAAAGCATA CAGAGTACAG
    AGAGAAGGCT CCCTCCAGGT CGGCAACGAA GCTCCCATCA CTGGCTCTTC ACCGCTGGGT
    GCCACACAGC TGGACACGGA TGGGGCCCTG TGGCTGGGGG GAATGGAGCG GCTCAGCGTG
    GCTCACAAGC TGCCCAAGGC TTACAGCACT GGCTTTATTG GCTGTATAAG GGACGTGATT
    GTCGACCGCC AAGAACTGCA TCTGGTGGAG GACGCTTTAA ACAACCCCAC GATATTACAC
    TGCTCAGCCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45013
    Clone ID Related Accession (Same CDS sequence) XM_015296840.1
    Accession Version XM_015296840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product agrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGGTGCCT CACGCCTTCC TCAACCTGAA GGAGCCATTC
    TACGTGGGGG GAGCCCCCGA TTTCAGCAAG CTGGCTCGAG CTGCAGCCAT CTCCACCAGC
    TTCTATGGAG CTGTGCAGAG GATCTCCATC AAAGGGGTTC CCTTGCTGAA GGAGCAGCAC
    ATCCGCAGCG CCGTTGAAAT CTCCACTTTC CGTGCCCACC CCTGCACCCA AAAGCCCAAC
    CCGTGCCAGA ATGGAGGCAC CTGCAGCCCC CGGTTGGAGA GCTACGAGTG TGCCTGCCAG
    AGGGGCTTCT CTGGGGCTCA CTGCGAGAAA GTGATCATTG AGAAGGCAGC TGGAGATGCA
    GAGGCCATTG CATTTGATGG TAGGACGTAC ATGGAGTACC ACAATGCCGT GACAAAGAGC
    CACCTGAGCA ATGAGATCCC TGCTCCCGAC GCATTGGACT ACCCTGCTGA GCCCAGCGAG
    AAGGCTTTGC AATCCAACCA CTTTGAGCTG AGCATCAAAA CAGAAGCAAC CCAGGGCCTG
    ATCTTGTGGA GTGGGAAGGG GCTGGAGCGA TCCGACTACA TCGCCCTCGC CATCGTGGAT
    GGCTTCGTGC AGATGATGTA TGACCTGGGC TCCAAGCCAG TCGTCCTGCG GTCCACAGTC
    CCCATCAACA CCAACCACTG GACCCACATC AAAGCATACA GAGTACAGAG AGAAGGCTCC
    CTCCAGGTCG GCAACGAAGC TCCCATCACT GGCTCTTCAC CGCTGGGTGC CACACAGCTG
    GACACGGATG GGGCCCTGTG GCTGGGGGGA ATGGAGCGGC TCAGCGTGGC TCACAAGCTG
    CCCAAGGCTT ACAGCACTGG CTTTATTGGC TGTATAAGGG ACGTGATTGT CGACCGCCAA
    GAACTGCATC TGGTGGAGGA CGCTTTAAAC AACCCCACGA TATTACACTG CTCAGCCAAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152326.1
    CDS171..6413
    Translation

    Target ORF information:

    RefSeq Version XM_015296840.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGGTGCCT CACGCCTTCC TCAACCTGAA GGAGCCATTC
    TACGTGGGGG GAGCCCCCGA TTTCAGCAAG CTGGCTCGAG CTGCAGCCAT CTCCACCAGC
    TTCTATGGAG CTGTGCAGAG GATCTCCATC AAAGGGGTTC CCTTGCTGAA GGAGCAGCAC
    ATCCGCAGCG CCGTTGAAAT CTCCACTTTC CGTGCCCACC CCTGCACCCA AAAGCCCAAC
    CCGTGCCAGA ATGGAGGCAC CTGCAGCCCC CGGTTGGAGA GCTACGAGTG TGCCTGCCAG
    AGGGGCTTCT CTGGGGCTCA CTGCGAGAAA GTGATCATTG AGAAGGCAGC TGGAGATGCA
    GAGGCCATTG CATTTGATGG TAGGACGTAC ATGGAGTACC ACAATGCCGT GACAAAGAGC
    CACCTGAGCA ATGAGATCCC TGCTCCCGAC GCATTGGACT ACCCTGCTGA GCCCAGCGAG
    AAGGCTTTGC AATCCAACCA CTTTGAGCTG AGCATCAAAA CAGAAGCAAC CCAGGGCCTG
    ATCTTGTGGA GTGGGAAGGG GCTGGAGCGA TCCGACTACA TCGCCCTCGC CATCGTGGAT
    GGCTTCGTGC AGATGATGTA TGACCTGGGC TCCAAGCCAG TCGTCCTGCG GTCCACAGTC
    CCCATCAACA CCAACCACTG GACCCACATC AAAGCATACA GAGTACAGAG AGAAGGCTCC
    CTCCAGGTCG GCAACGAAGC TCCCATCACT GGCTCTTCAC CGCTGGGTGC CACACAGCTG
    GACACGGATG GGGCCCTGTG GCTGGGGGGA ATGGAGCGGC TCAGCGTGGC TCACAAGCTG
    CCCAAGGCTT ACAGCACTGG CTTTATTGGC TGTATAAGGG ACGTGATTGT CGACCGCCAA
    GAACTGCATC TGGTGGAGGA CGCTTTAAAC AACCCCACGA TATTACACTG CTCAGCCAAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45014
    Clone ID Related Accession (Same CDS sequence) XM_015296841.1
    Accession Version XM_015296841.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product agrin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAC AAGCCCAATA GCTATTTTAC CCAGACCCCG
    CCGACCCCTC GAGATGCCTG CCGAGGGATG CTGTGTGGGT TCGGGGCGGT GTGCGAGCGC
    AGCCCCACTG ACCCCTCCCA AGCCTCCTGT GTCTGCAAGA AGACAGCTTG CCCTGTGGTG
    GTGGCTCCTG TCTGCGGCTC CGACTATTCC ACCTACAGCA ACGAGTGTGA GCTGGAGAAA
    GCCCAGTGCA ACCAGCAGAG GCGGATCAAG GTCATCAGCA AGGGACCATG TGGCTCCAAG
    GACCCCTGTG CAGAGGTGAC GTGCAGCTTT GGCAGCACGT GCGTCCGCTC TGCAGATGGC
    CAGACGGCCA GGTGTGTGTG CCCCGCATCC TGCAGCGGTG TGGCTGAGAG CATCGTCTGC
    GGCAGTGATG GCAAGGACTA CCGCAGTGAG TGCGACCTCA ACAAGCACGC CTGTGACAAG
    CAGGAGAATG TCTTCAAGAA GTTTGATGGA GCCTGCGACC CCTGTAAGGG CATCCTCAAC
    GACATGAACC GTGTGTGCCG GGTGAACCCC CGCACCCGCC GGGTGGAGCT GCTCTCCCGC
    CCCGAGAACT GTCCCTCAAA GAGGGAGCCA GTGTGCGGTG ACGATGGGGT GACGTATGCC
    AGTGAGTGTG TGATGGGGCG CACGGGGGCC ATCCGGGGGC TGGAGATCCA GAAGGTGCGC
    TCTGGGCAGT GCCAGCACCA GGACAAATGC AAGGATGAAT GCAAGTTCAA TGCTGTCTGC
    CTGAACCGCC GTGGCACGGC GCGCTGCTCC TGCGACCGCA TCACTTGTGA TGGCACCTAC
    CGGCCTGTCT GTGCCCGCGA CAGCCGCACC TACAGCAACG ACTGTGAGCG GCAGAAAGCC
    GAGTGCCACC AGAAAGCTGC CATCCCAGTG AAGCACAGCG GACCCTGTGA CCTGGGTACT
    CCCAGCCCCT GCCTGAGCGT GGAGTGCACT TTTGGGGCCA CCTGCGTGGT GAAGAACCGG
    GAGCCCGTGT GCGAGTGCCA GCAGGTGTGC CAGGGCCGCT ATGACCCCGT CTGCGGCAGC
    GACAACCGCA CCTACGGCAA CCCCTGCGAG CTCAACGCCA TGGCCTGCGT CCTCAAGAGA
    GAGATCAGGG TGAAGCACAA GGGGCCCTGC GACCGCTGTG GCAAGTGCCA GTTTGGGGCC
    ATCTGCGAGG CGGAGACGGG GCGGTGCGTG TGCCCCACGG AGTGCGTGCC CTCCTCCCAG
    CCTGTCTGTG GCACAGATGG CAACACCTAC GGCAGCGAGT GTGAGCTCCA CGTCCGAGCG
    TGCACGCAGC AGAAGAACAT TTTGGTGGCT GCCCAAGGCG ACTGCAAGTC CTGCGGCAGC
    ACGGTGTGCT CCTTCGGCAG CACGTGTGTG GGTGGGCAGT GCGTGTGTCC CCGCTGTGAG
    CAGCAGCCCC TGGCCCAGGT CTGTGGCACC GATGGCCTCA CCTATGACAA CCGCTGCGAG
    CTCCGAGCAG CCTCCTGCCA ACAGCAGAAG AGCATCGAGG TGGCCAAGAT GGGGCCGTGT
    GAGGACGAAT GTGGCTCAGG GGGCTCAGGC TCTGGTGATG GGAGTGAGTG TGAGCAGGAC
    CGGTGCCGGC ACTACGGGGG CTGGTGGGAT GAGGATGCAG AGGACGACCG CTGTGTGTGT
    GACTTCACCT GCCTGGCGGT GCCACGCAGC CCGGTCTGTG GCTCTGACGA TGTGACCTAC
    GCCAATGAGT GTGAGCTGAA GAAGACGAGG TGTGAAAAAC GCCAGGACCT CTATGTCACC
    AGCCAAGGAG CCTGCCGTGC CCTCACCACG ACCCCACCGC CTCTCCCAGT TGTGCACTGC
    AGTCAGACCA TCTATGGGTG CTGCCCGGAC AACATGACCC TGGCGCTCGG AGTGGGTGCA
    GCCGGGTGCC CAAGCACCTG CCAGTGCAAC CCCTATGGCT CCTATGGAGG AACCTGCGAC
    CCTGCCACGG GGCAGTGCTC CTGCAAGCCA GGTGTTGGGG GGCTCAAGTG TGACCGCTGT
    GAGCCTGGCT TCTGGAACTT CCGTGGCATC GTCACCGACA GCAAGAGCGG CTGCACGCCC
    TGTAACTGCG ACCCGGTGGG CTCAGTGCGT GATGACTGCG AGCAGATGAC TGGGCTCTGC
    TCCTGCAAGA CTGGGATCAC TGGCATGAAG TGCAACCAGT GTCCCAACGG CAGCAAGATG
    GGCATGGCTG GCTGTGAAAA AGACCCATCA GCTCCAAAAT CCTGTGAGGA AATGAGCTGC
    GAGTTCGGGG CTACGTGTGT GGAGGTCAAC GGCTTTGCCC ACTGCGAGTG CCCGTCCCCA
    CTCTGCTCAG AGGCCAACAT GACCAAGGTG TGTGGCTCTG ATGGTGTTAC CTATGGGGAC
    CAGTGCCAGC TGAAGACCAT TGCGTGCCGG CAGGGACAGC TCATCACAGT GAAACACGTG
    GGGCAATGCC ACGAGTCTAT AACTCACACG AGCCATACGA TGCCACCCAC CCCTCTGCCC
    ACCTTGCCCT TGGACAAGCT CATCGTACCC CCACCACTGC AGCTGACCAC CCAGGCTCCA
    GAGCCCACTG AGCTGGCCAC CACCTCCCTG CTGATGGAAG CCAGCCCCAC TACAAGAAGT
    CACCCTACAA CAAGGCGTGT CACAACGACC CGACCTGTCA CCACACCATG GATGACCCAT
    GGAGTGCTGA AGACCACCGT CCGCCCACTC TCCACCTCTC CAGTGGTCCT GGCCACCACT
    CAGCCTCCCT ACGCTGAATC GGGCAGTGCA GAAGGCAGTG GGGACCAGGA GATGAGCATC
    AGTGGGGACC AGGAATCCAG TGGGGCAGGC TCTGCTGGGG AAGAGGAGGT GGAGGAAAGC
    CAGGTAACCC CAACTCCAGC CATTGAGAGG GCAACGTGCT ACAACACCCC ACTCGGGTGC
    TGCTCTGATG GCAAGACTGC AGCTGCTGAT GCAGAAGGGA GCAACTGTCC GGCTACCAAA
    GTCTTCCAAG GAGTCCTCAT CTTGGAGGAG GTGGAAGGCC AGGAGCTGTT CTACACACCT
    GAGATGGCTG ACCCCAAGTC AGAGCTCTTC GGGGAGACAG CCAGGAGCAT CGAGAGCGCA
    CTGGACGAGC TTTTCCGAAA TTCTGATGTT AAGAATGATT TCAAGAGCAT CCGTGTCCGA
    GACCTGGGGC AGAGCAGCGC TGTCCGTGTC ATTGTGGAGT CCCACTTTGA CCCAGCCACT
    TCCTACACAG CAGCTGACGT TCAGGCGGCC CTGCTGAAGC AGATCAGAGC CTCCAAGAAG
    AGGACCATCC TGGTGAAGAA GCCCCAGCAG GAGCATGTCA AATTCATGGA CTTCGACTGG
    ATCCCCAGGA TCTTCACCAC CACCATCACC ACCACCACGG CCACCACCAT GGCACCTGCC
    ACCACACGGA GGCACACCAC AGCCTCTGCT GCCACCACTG CCCACATCCT GCGCCAGGAC
    ACCGTGGGTC ACCCCAGTGC CAAGCTGGCA GCACCTGCAA GCACCAGGAG GCCTACATCC
    ACCCTGCCTA CCACCGCCCG GCGCAAACCC ACTCGCCAGC CCCCCAGCAC CACCAAGAAG
    CCCTCACGAC CCTGCGACTC ACACCCCTGC CTGCATGGTG GCACCTGTGA GGACGATGGC
    AAGGAGTTCA CCTGCAGCTG CCCAGCAGGC AAAGGAGGAG CTGTGTGTGA GAAACCCATC
    AGGTACTTCA TCCCCAGCTT CGGTGGCAAG TCCTACCTGG CCTTCAAGAT GATGAAGGCG
    TACCACACCG TGCGCATCGC CATGGAGTTC AGGGCCACGG AGCTCAGCGG GCTGCTGCTC
    TACAACGGGC AGAATCGTGG CAAGGACTTC ATCTCCCTGG CGCTGGTGGG CGGCTTCGTG
    GAGCTCAGGT TTAACACAGG CTCTGGCACG GGCGTCATCA CCAGCAAGGT GCGCGTGGAG
    CCTGGCAAGT GGCACCAGCT GGTGGTGAAC CGCAACCGCC GCAGCGGCAT GCTGGCTGTG
    GATGGGGAGC CCCACGTCAG TGGGGAGAGC CCCACGGGTA CCGATGGGCT CAACCTCGAC
    ACCGACCTCT TTGTTGGAGG AGCTCCCGAG GACCAAATGG CTGTAGTGGC AGAGCGTACT
    GCGGCTACGG TTGGCCTGAA GGGCTGCATC CGCCTCCTGG ATGTGAACAA CCAGATGTAC
    GACCTGAGGG AGAAAGGCAG CGATGTGCTG TATGGCAGTG GGGTGGGCGA GTGTGGCAAC
    GACCCCTGCC ACCCCAACCC CTGCCACCAT GGGGGCATCT GCCACGTCAA GGAGGCAGAG
    ATGTTCCACT GCGAGTGTCT CCACTCCTAC ACCGGTCCAA CATGTGCTGA TGAGAGGAAC
    CCATGCGATC CAACGCCGTG CCACATCTCT GCCACCTGCC TGGTGCTGCC GGAGGGAGGT
    GCCATGTGTG CCTGTCCCAT GGGACGGGAA GGAGAGTTCT GTGAGCGAGT GACGGAGCAG
    GACCACACCA TGCCCTTCCT CCCAGAGTTC AATGGCTTTT CCTACCTGGA GCTGAACGGG
    CTGCAGACCT TTGTTCCTGA CCTGCAGGAC AAAATGTCCA TGGAAGTGGT GTTCCTGGCC
    AAGAGTCCCA GTGGCATGAT CTTCTACAAT GGGCAGAAGA CAGATGGCAA AGGAGACTTT
    GTGTCTCTGG CTTTGCACGA CGGCTACCTG GAGTATCGAT ATGACCTGGG CAAAGGGGCA
    GCTGTGCTCA GGAGCAAAGA GCCAGTTCCC CTCAACACCT GGATAAGCGT TTTGTTGGAG
    AGGAGTGGGC GCAAAGGGGT AATGAGGATC AACAATGGCG AGAGGGTGAT GGGAGAGTCA
    CCGAAATCCC GTAAGGTGCC TCACGCCTTC CTCAACCTGA AGGAGCCATT CTACGTGGGG
    GGAGCCCCCG ATTTCAGCAA GCTGGCTCGA GCTGCAGCCA TCTCCACCAG CTTCTATGGA
    GCTGTGCAGA GGATCTCCAT CAAAGGGGTT CCCTTGCTGA AGGAGCAGCA CATCCGCAGC
    GCCGTTGAAA TCTCCACTTT CCGTGCCCAC CCCTGCACCC AAAAGCCCAA CCCGTGCCAG
    AATGGAGGCA CCTGCAGCCC CCGGTTGGAG AGCTACGAGT GTGCCTGCCA GAGGGGCTTC
    TCTGGGGCTC ACTGCGAGAA AGTGATCATT GAGAAGGCAG CTGGAGATGC AGAGGCCATT
    GCATTTGATG GTAGGACGTA CATGGAGTAC CACAATGCCG TGACAAAGAG CCACCTGAGC
    AATGAGATCC CTGCTCCCGA CGCATTGGAC TACCCTGCTG AGCCCAGCGA GAAGGCTTTG
    CAATCCAACC ACTTTGAGCT GAGCATCAAA ACAGAAGCAA CCCAGGGCCT GATCTTGTGG
    AGTGGGAAGG GGCTGGAGCG ATCCGACTAC ATCGCCCTCG CCATCGTGGA TGGCTTCGTG
    CAGATGATGT ATGACCTGGG CTCCAAGCCA GTCGTCCTGC GGTCCACAGT CCCCATCAAC
    ACCAACCACT GGACCCACAT CAAAGCATAC AGAGTACAGA GAGAAGGCTC CCTCCAGGTC
    GGCAACGAAG CTCCCATCAC TGGCTCTTCA CCGCTGGGTG CCACACAGCT GGACACGGAT
    GGGGCCCTGT GGCTGGGGGG AATGGAGCGG CTCAGCGTGG CTCACAAGCT GCCCAAGGCT
    TACAGCACTG GCTTTATTGG CTGTATAAGG GACGTGATTG TCGACCGCCA AGAACTGCAT
    CTGGTGGAGG ACGCTTTAAA CAACCCCACG ATATTACACT GCTCAGCCAA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152327.1
    CDS172..6405
    Translation

    Target ORF information:

    RefSeq Version XM_015296841.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296841.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAC AAGCCCAATA GCTATTTTAC CCAGACCCCG
    CCGACCCCTC GAGATGCCTG CCGAGGGATG CTGTGTGGGT TCGGGGCGGT GTGCGAGCGC
    AGCCCCACTG ACCCCTCCCA AGCCTCCTGT GTCTGCAAGA AGACAGCTTG CCCTGTGGTG
    GTGGCTCCTG TCTGCGGCTC CGACTATTCC ACCTACAGCA ACGAGTGTGA GCTGGAGAAA
    GCCCAGTGCA ACCAGCAGAG GCGGATCAAG GTCATCAGCA AGGGACCATG TGGCTCCAAG
    GACCCCTGTG CAGAGGTGAC GTGCAGCTTT GGCAGCACGT GCGTCCGCTC TGCAGATGGC
    CAGACGGCCA GGTGTGTGTG CCCCGCATCC TGCAGCGGTG TGGCTGAGAG CATCGTCTGC
    GGCAGTGATG GCAAGGACTA CCGCAGTGAG TGCGACCTCA ACAAGCACGC CTGTGACAAG
    CAGGAGAATG TCTTCAAGAA GTTTGATGGA GCCTGCGACC CCTGTAAGGG CATCCTCAAC
    GACATGAACC GTGTGTGCCG GGTGAACCCC CGCACCCGCC GGGTGGAGCT GCTCTCCCGC
    CCCGAGAACT GTCCCTCAAA GAGGGAGCCA GTGTGCGGTG ACGATGGGGT GACGTATGCC
    AGTGAGTGTG TGATGGGGCG CACGGGGGCC ATCCGGGGGC TGGAGATCCA GAAGGTGCGC
    TCTGGGCAGT GCCAGCACCA GGACAAATGC AAGGATGAAT GCAAGTTCAA TGCTGTCTGC
    CTGAACCGCC GTGGCACGGC GCGCTGCTCC TGCGACCGCA TCACTTGTGA TGGCACCTAC
    CGGCCTGTCT GTGCCCGCGA CAGCCGCACC TACAGCAACG ACTGTGAGCG GCAGAAAGCC
    GAGTGCCACC AGAAAGCTGC CATCCCAGTG AAGCACAGCG GACCCTGTGA CCTGGGTACT
    CCCAGCCCCT GCCTGAGCGT GGAGTGCACT TTTGGGGCCA CCTGCGTGGT GAAGAACCGG
    GAGCCCGTGT GCGAGTGCCA GCAGGTGTGC CAGGGCCGCT ATGACCCCGT CTGCGGCAGC
    GACAACCGCA CCTACGGCAA CCCCTGCGAG CTCAACGCCA TGGCCTGCGT CCTCAAGAGA
    GAGATCAGGG TGAAGCACAA GGGGCCCTGC GACCGCTGTG GCAAGTGCCA GTTTGGGGCC
    ATCTGCGAGG CGGAGACGGG GCGGTGCGTG TGCCCCACGG AGTGCGTGCC CTCCTCCCAG
    CCTGTCTGTG GCACAGATGG CAACACCTAC GGCAGCGAGT GTGAGCTCCA CGTCCGAGCG
    TGCACGCAGC AGAAGAACAT TTTGGTGGCT GCCCAAGGCG ACTGCAAGTC CTGCGGCAGC
    ACGGTGTGCT CCTTCGGCAG CACGTGTGTG GGTGGGCAGT GCGTGTGTCC CCGCTGTGAG
    CAGCAGCCCC TGGCCCAGGT CTGTGGCACC GATGGCCTCA CCTATGACAA CCGCTGCGAG
    CTCCGAGCAG CCTCCTGCCA ACAGCAGAAG AGCATCGAGG TGGCCAAGAT GGGGCCGTGT
    GAGGACGAAT GTGGCTCAGG GGGCTCAGGC TCTGGTGATG GGAGTGAGTG TGAGCAGGAC
    CGGTGCCGGC ACTACGGGGG CTGGTGGGAT GAGGATGCAG AGGACGACCG CTGTGTGTGT
    GACTTCACCT GCCTGGCGGT GCCACGCAGC CCGGTCTGTG GCTCTGACGA TGTGACCTAC
    GCCAATGAGT GTGAGCTGAA GAAGACGAGG TGTGAAAAAC GCCAGGACCT CTATGTCACC
    AGCCAAGGAG CCTGCCGTGC CCTCACCACG ACCCCACCGC CTCTCCCAGT TGTGCACTGC
    AGTCAGACCA TCTATGGGTG CTGCCCGGAC AACATGACCC TGGCGCTCGG AGTGGGTGCA
    GCCGGGTGCC CAAGCACCTG CCAGTGCAAC CCCTATGGCT CCTATGGAGG AACCTGCGAC
    CCTGCCACGG GGCAGTGCTC CTGCAAGCCA GGTGTTGGGG GGCTCAAGTG TGACCGCTGT
    GAGCCTGGCT TCTGGAACTT CCGTGGCATC GTCACCGACA GCAAGAGCGG CTGCACGCCC
    TGTAACTGCG ACCCGGTGGG CTCAGTGCGT GATGACTGCG AGCAGATGAC TGGGCTCTGC
    TCCTGCAAGA CTGGGATCAC TGGCATGAAG TGCAACCAGT GTCCCAACGG CAGCAAGATG
    GGCATGGCTG GCTGTGAAAA AGACCCATCA GCTCCAAAAT CCTGTGAGGA AATGAGCTGC
    GAGTTCGGGG CTACGTGTGT GGAGGTCAAC GGCTTTGCCC ACTGCGAGTG CCCGTCCCCA
    CTCTGCTCAG AGGCCAACAT GACCAAGGTG TGTGGCTCTG ATGGTGTTAC CTATGGGGAC
    CAGTGCCAGC TGAAGACCAT TGCGTGCCGG CAGGGACAGC TCATCACAGT GAAACACGTG
    GGGCAATGCC ACGAGTCTAT AACTCACACG AGCCATACGA TGCCACCCAC CCCTCTGCCC
    ACCTTGCCCT TGGACAAGCT CATCGTACCC CCACCACTGC AGCTGACCAC CCAGGCTCCA
    GAGCCCACTG AGCTGGCCAC CACCTCCCTG CTGATGGAAG CCAGCCCCAC TACAAGAAGT
    CACCCTACAA CAAGGCGTGT CACAACGACC CGACCTGTCA CCACACCATG GATGACCCAT
    GGAGTGCTGA AGACCACCGT CCGCCCACTC TCCACCTCTC CAGTGGTCCT GGCCACCACT
    CAGCCTCCCT ACGCTGAATC GGGCAGTGCA GAAGGCAGTG GGGACCAGGA GATGAGCATC
    AGTGGGGACC AGGAATCCAG TGGGGCAGGC TCTGCTGGGG AAGAGGAGGT GGAGGAAAGC
    CAGGTAACCC CAACTCCAGC CATTGAGAGG GCAACGTGCT ACAACACCCC ACTCGGGTGC
    TGCTCTGATG GCAAGACTGC AGCTGCTGAT GCAGAAGGGA GCAACTGTCC GGCTACCAAA
    GTCTTCCAAG GAGTCCTCAT CTTGGAGGAG GTGGAAGGCC AGGAGCTGTT CTACACACCT
    GAGATGGCTG ACCCCAAGTC AGAGCTCTTC GGGGAGACAG CCAGGAGCAT CGAGAGCGCA
    CTGGACGAGC TTTTCCGAAA TTCTGATGTT AAGAATGATT TCAAGAGCAT CCGTGTCCGA
    GACCTGGGGC AGAGCAGCGC TGTCCGTGTC ATTGTGGAGT CCCACTTTGA CCCAGCCACT
    TCCTACACAG CAGCTGACGT TCAGGCGGCC CTGCTGAAGC AGATCAGAGC CTCCAAGAAG
    AGGACCATCC TGGTGAAGAA GCCCCAGCAG GAGCATGTCA AATTCATGGA CTTCGACTGG
    ATCCCCAGGA TCTTCACCAC CACCATCACC ACCACCACGG CCACCACCAT GGCACCTGCC
    ACCACACGGA GGCACACCAC AGCCTCTGCT GCCACCACTG CCCACATCCT GCGCCAGGAC
    ACCGTGGGTC ACCCCAGTGC CAAGCTGGCA GCACCTGCAA GCACCAGGAG GCCTACATCC
    ACCCTGCCTA CCACCGCCCG GCGCAAACCC ACTCGCCAGC CCCCCAGCAC CACCAAGAAG
    CCCTCACGAC CCTGCGACTC ACACCCCTGC CTGCATGGTG GCACCTGTGA GGACGATGGC
    AAGGAGTTCA CCTGCAGCTG CCCAGCAGGC AAAGGAGGAG CTGTGTGTGA GAAACCCATC
    AGGTACTTCA TCCCCAGCTT CGGTGGCAAG TCCTACCTGG CCTTCAAGAT GATGAAGGCG
    TACCACACCG TGCGCATCGC CATGGAGTTC AGGGCCACGG AGCTCAGCGG GCTGCTGCTC
    TACAACGGGC AGAATCGTGG CAAGGACTTC ATCTCCCTGG CGCTGGTGGG CGGCTTCGTG
    GAGCTCAGGT TTAACACAGG CTCTGGCACG GGCGTCATCA CCAGCAAGGT GCGCGTGGAG
    CCTGGCAAGT GGCACCAGCT GGTGGTGAAC CGCAACCGCC GCAGCGGCAT GCTGGCTGTG
    GATGGGGAGC CCCACGTCAG TGGGGAGAGC CCCACGGGTA CCGATGGGCT CAACCTCGAC
    ACCGACCTCT TTGTTGGAGG AGCTCCCGAG GACCAAATGG CTGTAGTGGC AGAGCGTACT
    GCGGCTACGG TTGGCCTGAA GGGCTGCATC CGCCTCCTGG ATGTGAACAA CCAGATGTAC
    GACCTGAGGG AGAAAGGCAG CGATGTGCTG TATGGCAGTG GGGTGGGCGA GTGTGGCAAC
    GACCCCTGCC ACCCCAACCC CTGCCACCAT GGGGGCATCT GCCACGTCAA GGAGGCAGAG
    ATGTTCCACT GCGAGTGTCT CCACTCCTAC ACCGGTCCAA CATGTGCTGA TGAGAGGAAC
    CCATGCGATC CAACGCCGTG CCACATCTCT GCCACCTGCC TGGTGCTGCC GGAGGGAGGT
    GCCATGTGTG CCTGTCCCAT GGGACGGGAA GGAGAGTTCT GTGAGCGAGT GACGGAGCAG
    GACCACACCA TGCCCTTCCT CCCAGAGTTC AATGGCTTTT CCTACCTGGA GCTGAACGGG
    CTGCAGACCT TTGTTCCTGA CCTGCAGGAC AAAATGTCCA TGGAAGTGGT GTTCCTGGCC
    AAGAGTCCCA GTGGCATGAT CTTCTACAAT GGGCAGAAGA CAGATGGCAA AGGAGACTTT
    GTGTCTCTGG CTTTGCACGA CGGCTACCTG GAGTATCGAT ATGACCTGGG CAAAGGGGCA
    GCTGTGCTCA GGAGCAAAGA GCCAGTTCCC CTCAACACCT GGATAAGCGT TTTGTTGGAG
    AGGAGTGGGC GCAAAGGGGT AATGAGGATC AACAATGGCG AGAGGGTGAT GGGAGAGTCA
    CCGAAATCCC GTAAGGTGCC TCACGCCTTC CTCAACCTGA AGGAGCCATT CTACGTGGGG
    GGAGCCCCCG ATTTCAGCAA GCTGGCTCGA GCTGCAGCCA TCTCCACCAG CTTCTATGGA
    GCTGTGCAGA GGATCTCCAT CAAAGGGGTT CCCTTGCTGA AGGAGCAGCA CATCCGCAGC
    GCCGTTGAAA TCTCCACTTT CCGTGCCCAC CCCTGCACCC AAAAGCCCAA CCCGTGCCAG
    AATGGAGGCA CCTGCAGCCC CCGGTTGGAG AGCTACGAGT GTGCCTGCCA GAGGGGCTTC
    TCTGGGGCTC ACTGCGAGAA AGTGATCATT GAGAAGGCAG CTGGAGATGC AGAGGCCATT
    GCATTTGATG GTAGGACGTA CATGGAGTAC CACAATGCCG TGACAAAGAG CCACCTGAGC
    AATGAGATCC CTGCTCCCGA CGCATTGGAC TACCCTGCTG AGCCCAGCGA GAAGGCTTTG
    CAATCCAACC ACTTTGAGCT GAGCATCAAA ACAGAAGCAA CCCAGGGCCT GATCTTGTGG
    AGTGGGAAGG GGCTGGAGCG ATCCGACTAC ATCGCCCTCG CCATCGTGGA TGGCTTCGTG
    CAGATGATGT ATGACCTGGG CTCCAAGCCA GTCGTCCTGC GGTCCACAGT CCCCATCAAC
    ACCAACCACT GGACCCACAT CAAAGCATAC AGAGTACAGA GAGAAGGCTC CCTCCAGGTC
    GGCAACGAAG CTCCCATCAC TGGCTCTTCA CCGCTGGGTG CCACACAGCT GGACACGGAT
    GGGGCCCTGT GGCTGGGGGG AATGGAGCGG CTCAGCGTGG CTCACAAGCT GCCCAAGGCT
    TACAGCACTG GCTTTATTGG CTGTATAAGG GACGTGATTG TCGACCGCCA AGAACTGCAT
    CTGGTGGAGG ACGCTTTAAA CAACCCCACG ATATTACACT GCTCAGCCAA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45015
    Clone ID Related Accession (Same CDS sequence) XM_015296842.1 , XM_015296842.2
    Accession Version XM_015296842.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product agrin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCGAGAAGGC TTTGCAATCC AACCACTTTG AGCTGAGCAT CAAAACAGAA
    GCAACCCAGG GCCTGATCTT GTGGAGTGGG AAGGGGCTGG AGCGATCCGA CTACATCGCC
    CTCGCCATCG TGGATGGCTT CGTGCAGATG ATGTATGACC TGGGCTCCAA GCCAGTCGTC
    CTGCGGTCCA CAGTCCCCAT CAACACCAAC CACTGGACCC ACATCAAAGC ATACAGAGTA
    CAGAGAGAAG GCTCCCTCCA GGTCGGCAAC GAAGCTCCCA TCACTGGCTC TTCACCGCTG
    GGTGCCACAC AGCTGGACAC GGATGGGGCC CTGTGGCTGG GGGGAATGGA GCGGCTCAGC
    GTGGCTCACA AGCTGCCCAA GGCTTACAGC ACTGGCTTTA TTGGCTGTAT AAGGGACGTG
    ATTGTCGACC GCCAAGAACT GCATCTGGTG GAGGACGCTT TAAACAACCC CACGATATTA
    CACTGCTCAG CCAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152328.1
    CDS170..6367
    Translation

    Target ORF information:

    RefSeq Version XM_015296842.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296842.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCGAGAAGGC TTTGCAATCC AACCACTTTG AGCTGAGCAT CAAAACAGAA
    GCAACCCAGG GCCTGATCTT GTGGAGTGGG AAGGGGCTGG AGCGATCCGA CTACATCGCC
    CTCGCCATCG TGGATGGCTT CGTGCAGATG ATGTATGACC TGGGCTCCAA GCCAGTCGTC
    CTGCGGTCCA CAGTCCCCAT CAACACCAAC CACTGGACCC ACATCAAAGC ATACAGAGTA
    CAGAGAGAAG GCTCCCTCCA GGTCGGCAAC GAAGCTCCCA TCACTGGCTC TTCACCGCTG
    GGTGCCACAC AGCTGGACAC GGATGGGGCC CTGTGGCTGG GGGGAATGGA GCGGCTCAGC
    GTGGCTCACA AGCTGCCCAA GGCTTACAGC ACTGGCTTTA TTGGCTGTAT AAGGGACGTG
    ATTGTCGACC GCCAAGAACT GCATCTGGTG GAGGACGCTT TAAACAACCC CACGATATTA
    CACTGCTCAG CCAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45015
    Clone ID Related Accession (Same CDS sequence) XM_015296842.1 , XM_015296842.2
    Accession Version XM_015296842.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product agrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCGAGAAGGC TTTGCAATCC AACCACTTTG AGCTGAGCAT CAAAACAGAA
    GCAACCCAGG GCCTGATCTT GTGGAGTGGG AAGGGGCTGG AGCGATCCGA CTACATCGCC
    CTCGCCATCG TGGATGGCTT CGTGCAGATG ATGTATGACC TGGGCTCCAA GCCAGTCGTC
    CTGCGGTCCA CAGTCCCCAT CAACACCAAC CACTGGACCC ACATCAAAGC ATACAGAGTA
    CAGAGAGAAG GCTCCCTCCA GGTCGGCAAC GAAGCTCCCA TCACTGGCTC TTCACCGCTG
    GGTGCCACAC AGCTGGACAC GGATGGGGCC CTGTGGCTGG GGGGAATGGA GCGGCTCAGC
    GTGGCTCACA AGCTGCCCAA GGCTTACAGC ACTGGCTTTA TTGGCTGTAT AAGGGACGTG
    ATTGTCGACC GCCAAGAACT GCATCTGGTG GAGGACGCTT TAAACAACCC CACGATATTA
    CACTGCTCAG CCAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152328.1
    CDS188..6385
    Translation

    Target ORF information:

    RefSeq Version XM_015296842.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296842.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GCGAGAAGGC TTTGCAATCC AACCACTTTG AGCTGAGCAT CAAAACAGAA
    GCAACCCAGG GCCTGATCTT GTGGAGTGGG AAGGGGCTGG AGCGATCCGA CTACATCGCC
    CTCGCCATCG TGGATGGCTT CGTGCAGATG ATGTATGACC TGGGCTCCAA GCCAGTCGTC
    CTGCGGTCCA CAGTCCCCAT CAACACCAAC CACTGGACCC ACATCAAAGC ATACAGAGTA
    CAGAGAGAAG GCTCCCTCCA GGTCGGCAAC GAAGCTCCCA TCACTGGCTC TTCACCGCTG
    GGTGCCACAC AGCTGGACAC GGATGGGGCC CTGTGGCTGG GGGGAATGGA GCGGCTCAGC
    GTGGCTCACA AGCTGCCCAA GGCTTACAGC ACTGGCTTTA TTGGCTGTAT AAGGGACGTG
    ATTGTCGACC GCCAAGAACT GCATCTGGTG GAGGACGCTT TAAACAACCC CACGATATTA
    CACTGCTCAG CCAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45016
    Clone ID Related Accession (Same CDS sequence) XM_015296843.2 , XM_015296843.1
    Accession Version XM_015296843.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product agrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGGTGCCT CACGCCTTCC TCAACCTGAA GGAGCCATTC
    TACGTGGGGG GAGCCCCCGA TTTCAGCAAG CTGGCTCGAG CTGCAGCCAT CTCCACCAGC
    TTCTATGGAG CTGTGCAGAG GATCTCCATC AAAGGGGTTC CCTTGCTGAA GGAGCAGCAC
    ATCCGCAGCG CCGTTGAAAT CTCCACTTTC CGTGCCCACC CCTGCACCCA AAAGCCCAAC
    CCGTGCCAGA ATGGAGGCAC CTGCAGCCCC CGGTTGGAGA GCTACGAGTG TGCCTGCCAG
    AGGGGCTTCT CTGGGGCTCA CTGCGAGAAA GTGATCATTG AGAAGGCAGC TGGAGATGCA
    GAGGCCATTG CATTTGATGG TAGGACGTAC ATGGAGTACC ACAATGCCGT GACAAAGAGC
    GAGAAGGCTT TGCAATCCAA CCACTTTGAG CTGAGCATCA AAACAGAAGC AACCCAGGGC
    CTGATCTTGT GGAGTGGGAA GGGGCTGGAG CGATCCGACT ACATCGCCCT CGCCATCGTG
    GATGGCTTCG TGCAGATGAT GTATGACCTG GGCTCCAAGC CAGTCGTCCT GCGGTCCACA
    GTCCCCATCA ACACCAACCA CTGGACCCAC ATCAAAGCAT ACAGAGTACA GAGAGAAGGC
    TCCCTCCAGG TCGGCAACGA AGCTCCCATC ACTGGCTCTT CACCGCTGGG TGCCACACAG
    CTGGACACGG ATGGGGCCCT GTGGCTGGGG GGAATGGAGC GGCTCAGCGT GGCTCACAAG
    CTGCCCAAGG CTTACAGCAC TGGCTTTATT GGCTGTATAA GGGACGTGAT TGTCGACCGC
    CAAGAACTGC ATCTGGTGGA GGACGCTTTA AACAACCCCA CGATATTACA CTGCTCAGCC
    AAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152329.1
    CDS187..6372
    Translation

    Target ORF information:

    RefSeq Version XM_015296843.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296843.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGGTGCCT CACGCCTTCC TCAACCTGAA GGAGCCATTC
    TACGTGGGGG GAGCCCCCGA TTTCAGCAAG CTGGCTCGAG CTGCAGCCAT CTCCACCAGC
    TTCTATGGAG CTGTGCAGAG GATCTCCATC AAAGGGGTTC CCTTGCTGAA GGAGCAGCAC
    ATCCGCAGCG CCGTTGAAAT CTCCACTTTC CGTGCCCACC CCTGCACCCA AAAGCCCAAC
    CCGTGCCAGA ATGGAGGCAC CTGCAGCCCC CGGTTGGAGA GCTACGAGTG TGCCTGCCAG
    AGGGGCTTCT CTGGGGCTCA CTGCGAGAAA GTGATCATTG AGAAGGCAGC TGGAGATGCA
    GAGGCCATTG CATTTGATGG TAGGACGTAC ATGGAGTACC ACAATGCCGT GACAAAGAGC
    GAGAAGGCTT TGCAATCCAA CCACTTTGAG CTGAGCATCA AAACAGAAGC AACCCAGGGC
    CTGATCTTGT GGAGTGGGAA GGGGCTGGAG CGATCCGACT ACATCGCCCT CGCCATCGTG
    GATGGCTTCG TGCAGATGAT GTATGACCTG GGCTCCAAGC CAGTCGTCCT GCGGTCCACA
    GTCCCCATCA ACACCAACCA CTGGACCCAC ATCAAAGCAT ACAGAGTACA GAGAGAAGGC
    TCCCTCCAGG TCGGCAACGA AGCTCCCATC ACTGGCTCTT CACCGCTGGG TGCCACACAG
    CTGGACACGG ATGGGGCCCT GTGGCTGGGG GGAATGGAGC GGCTCAGCGT GGCTCACAAG
    CTGCCCAAGG CTTACAGCAC TGGCTTTATT GGCTGTATAA GGGACGTGAT TGTCGACCGC
    CAAGAACTGC ATCTGGTGGA GGACGCTTTA AACAACCCCA CGATATTACA CTGCTCAGCC
    AAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45016
    Clone ID Related Accession (Same CDS sequence) XM_015296843.2 , XM_015296843.1
    Accession Version XM_015296843.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product agrin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGGTGCCT CACGCCTTCC TCAACCTGAA GGAGCCATTC
    TACGTGGGGG GAGCCCCCGA TTTCAGCAAG CTGGCTCGAG CTGCAGCCAT CTCCACCAGC
    TTCTATGGAG CTGTGCAGAG GATCTCCATC AAAGGGGTTC CCTTGCTGAA GGAGCAGCAC
    ATCCGCAGCG CCGTTGAAAT CTCCACTTTC CGTGCCCACC CCTGCACCCA AAAGCCCAAC
    CCGTGCCAGA ATGGAGGCAC CTGCAGCCCC CGGTTGGAGA GCTACGAGTG TGCCTGCCAG
    AGGGGCTTCT CTGGGGCTCA CTGCGAGAAA GTGATCATTG AGAAGGCAGC TGGAGATGCA
    GAGGCCATTG CATTTGATGG TAGGACGTAC ATGGAGTACC ACAATGCCGT GACAAAGAGC
    GAGAAGGCTT TGCAATCCAA CCACTTTGAG CTGAGCATCA AAACAGAAGC AACCCAGGGC
    CTGATCTTGT GGAGTGGGAA GGGGCTGGAG CGATCCGACT ACATCGCCCT CGCCATCGTG
    GATGGCTTCG TGCAGATGAT GTATGACCTG GGCTCCAAGC CAGTCGTCCT GCGGTCCACA
    GTCCCCATCA ACACCAACCA CTGGACCCAC ATCAAAGCAT ACAGAGTACA GAGAGAAGGC
    TCCCTCCAGG TCGGCAACGA AGCTCCCATC ACTGGCTCTT CACCGCTGGG TGCCACACAG
    CTGGACACGG ATGGGGCCCT GTGGCTGGGG GGAATGGAGC GGCTCAGCGT GGCTCACAAG
    CTGCCCAAGG CTTACAGCAC TGGCTTTATT GGCTGTATAA GGGACGTGAT TGTCGACCGC
    CAAGAACTGC ATCTGGTGGA GGACGCTTTA AACAACCCCA CGATATTACA CTGCTCAGCC
    AAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152329.1
    CDS170..6355
    Translation

    Target ORF information:

    RefSeq Version XM_015296843.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296843.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGGTGCCT CACGCCTTCC TCAACCTGAA GGAGCCATTC
    TACGTGGGGG GAGCCCCCGA TTTCAGCAAG CTGGCTCGAG CTGCAGCCAT CTCCACCAGC
    TTCTATGGAG CTGTGCAGAG GATCTCCATC AAAGGGGTTC CCTTGCTGAA GGAGCAGCAC
    ATCCGCAGCG CCGTTGAAAT CTCCACTTTC CGTGCCCACC CCTGCACCCA AAAGCCCAAC
    CCGTGCCAGA ATGGAGGCAC CTGCAGCCCC CGGTTGGAGA GCTACGAGTG TGCCTGCCAG
    AGGGGCTTCT CTGGGGCTCA CTGCGAGAAA GTGATCATTG AGAAGGCAGC TGGAGATGCA
    GAGGCCATTG CATTTGATGG TAGGACGTAC ATGGAGTACC ACAATGCCGT GACAAAGAGC
    GAGAAGGCTT TGCAATCCAA CCACTTTGAG CTGAGCATCA AAACAGAAGC AACCCAGGGC
    CTGATCTTGT GGAGTGGGAA GGGGCTGGAG CGATCCGACT ACATCGCCCT CGCCATCGTG
    GATGGCTTCG TGCAGATGAT GTATGACCTG GGCTCCAAGC CAGTCGTCCT GCGGTCCACA
    GTCCCCATCA ACACCAACCA CTGGACCCAC ATCAAAGCAT ACAGAGTACA GAGAGAAGGC
    TCCCTCCAGG TCGGCAACGA AGCTCCCATC ACTGGCTCTT CACCGCTGGG TGCCACACAG
    CTGGACACGG ATGGGGCCCT GTGGCTGGGG GGAATGGAGC GGCTCAGCGT GGCTCACAAG
    CTGCCCAAGG CTTACAGCAC TGGCTTTATT GGCTGTATAA GGGACGTGAT TGTCGACCGC
    CAAGAACTGC ATCTGGTGGA GGACGCTTTA AACAACCCCA CGATATTACA CTGCTCAGCC
    AAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45018
    Clone ID Related Accession (Same CDS sequence) XM_015296845.1 , XM_015296845.2
    Accession Version XM_015296845.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product agrin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAC AAGCCCAATA GCTATTTTAC CCAGACCCCG
    CCGACCCCTC GAGATGCCTG CCGAGGGATG CTGTGTGGGT TCGGGGCGGT GTGCGAGCGC
    AGCCCCACTG ACCCCTCCCA AGCCTCCTGT GTCTGCAAGA AGACAGCTTG CCCTGTGGTG
    GTGGCTCCTG TCTGCGGCTC CGACTATTCC ACCTACAGCA ACGAGTGTGA GCTGGAGAAA
    GCCCAGTGCA ACCAGCAGAG GCGGATCAAG GTCATCAGCA AGGGACCATG TGGCTCCAAG
    GACCCCTGTG CAGAGGTGAC GTGCAGCTTT GGCAGCACGT GCGTCCGCTC TGCAGATGGC
    CAGACGGCCA GGTGTGTGTG CCCCGCATCC TGCAGCGGTG TGGCTGAGAG CATCGTCTGC
    GGCAGTGATG GCAAGGACTA CCGCAGTGAG TGCGACCTCA ACAAGCACGC CTGTGACAAG
    CAGGAGAATG TCTTCAAGAA GTTTGATGGA GCCTGCGACC CCTGTAAGGG CATCCTCAAC
    GACATGAACC GTGTGTGCCG GGTGAACCCC CGCACCCGCC GGGTGGAGCT GCTCTCCCGC
    CCCGAGAACT GTCCCTCAAA GAGGGAGCCA GTGTGCGGTG ACGATGGGGT GACGTATGCC
    AGTGAGTGTG TGATGGGGCG CACGGGGGCC ATCCGGGGGC TGGAGATCCA GAAGGTGCGC
    TCTGGGCAGT GCCAGCACCA GGACAAATGC AAGGATGAAT GCAAGTTCAA TGCTGTCTGC
    CTGAACCGCC GTGGCACGGC GCGCTGCTCC TGCGACCGCA TCACTTGTGA TGGCACCTAC
    CGGCCTGTCT GTGCCCGCGA CAGCCGCACC TACAGCAACG ACTGTGAGCG GCAGAAAGCC
    GAGTGCCACC AGAAAGCTGC CATCCCAGTG AAGCACAGCG GACCCTGTGA CCTGGGTACT
    CCCAGCCCCT GCCTGAGCGT GGAGTGCACT TTTGGGGCCA CCTGCGTGGT GAAGAACCGG
    GAGCCCGTGT GCGAGTGCCA GCAGGTGTGC CAGGGCCGCT ATGACCCCGT CTGCGGCAGC
    GACAACCGCA CCTACGGCAA CCCCTGCGAG CTCAACGCCA TGGCCTGCGT CCTCAAGAGA
    GAGATCAGGG TGAAGCACAA GGGGCCCTGC GACCGCTGTG GCAAGTGCCA GTTTGGGGCC
    ATCTGCGAGG CGGAGACGGG GCGGTGCGTG TGCCCCACGG AGTGCGTGCC CTCCTCCCAG
    CCTGTCTGTG GCACAGATGG CAACACCTAC GGCAGCGAGT GTGAGCTCCA CGTCCGAGCG
    TGCACGCAGC AGAAGAACAT TTTGGTGGCT GCCCAAGGCG ACTGCAAGTC CTGCGGCAGC
    ACGGTGTGCT CCTTCGGCAG CACGTGTGTG GGTGGGCAGT GCGTGTGTCC CCGCTGTGAG
    CAGCAGCCCC TGGCCCAGGT CTGTGGCACC GATGGCCTCA CCTATGACAA CCGCTGCGAG
    CTCCGAGCAG CCTCCTGCCA ACAGCAGAAG AGCATCGAGG TGGCCAAGAT GGGGCCGTGT
    GAGGACGAAT GTGGCTCAGG GGGCTCAGGC TCTGGTGATG GGAGTGAGTG TGAGCAGGAC
    CGGTGCCGGC ACTACGGGGG CTGGTGGGAT GAGGATGCAG AGGACGACCG CTGTGTGTGT
    GACTTCACCT GCCTGGCGGT GCCACGCAGC CCGGTCTGTG GCTCTGACGA TGTGACCTAC
    GCCAATGAGT GTGAGCTGAA GAAGACGAGG TGTGAAAAAC GCCAGGACCT CTATGTCACC
    AGCCAAGGAG CCTGCCGTGC CCTCACCACG ACCCCACCGC CTCTCCCAGT TGTGCACTGC
    AGTCAGACCA TCTATGGGTG CTGCCCGGAC AACATGACCC TGGCGCTCGG AGTGGGTGCA
    GCCGGGTGCC CAAGCACCTG CCAGTGCAAC CCCTATGGCT CCTATGGAGG AACCTGCGAC
    CCTGCCACGG GGCAGTGCTC CTGCAAGCCA GGTGTTGGGG GGCTCAAGTG TGACCGCTGT
    GAGCCTGGCT TCTGGAACTT CCGTGGCATC GTCACCGACA GCAAGAGCGG CTGCACGCCC
    TGTAACTGCG ACCCGGTGGG CTCAGTGCGT GATGACTGCG AGCAGATGAC TGGGCTCTGC
    TCCTGCAAGA CTGGGATCAC TGGCATGAAG TGCAACCAGT GTCCCAACGG CAGCAAGATG
    GGCATGGCTG GCTGTGAAAA AGACCCATCA GCTCCAAAAT CCTGTGAGGA AATGAGCTGC
    GAGTTCGGGG CTACGTGTGT GGAGGTCAAC GGCTTTGCCC ACTGCGAGTG CCCGTCCCCA
    CTCTGCTCAG AGGCCAACAT GACCAAGGTG TGTGGCTCTG ATGGTGTTAC CTATGGGGAC
    CAGTGCCAGC TGAAGACCAT TGCGTGCCGG CAGGGACAGC TCATCACAGT GAAACACGTG
    GGGCAATGCC ACGAGTCTAT AACTCACACG AGCCATACGA TGCCACCCAC CCCTCTGCCC
    ACCTTGCCCT TGGACAAGCT CATCGTACCC CCACCACTGC AGCTGACCAC CCAGGCTCCA
    GAGCCCACTG AGCTGGCCAC CACCTCCCTG CTGATGGAAG CCAGCCCCAC TACAAGAAGT
    CACCCTACAA CAAGGCGTGT CACAACGACC CGACCTGTCA CCACACCATG GATGACCCAT
    GGAGTGCTGA AGACCACCGT CCGCCCACTC TCCACCTCTC CAGTGGTCCT GGCCACCACT
    CAGCCTCCCT ACGCTGAATC GGGCAGTGCA GAAGGCAGTG GGGACCAGGA GATGAGCATC
    AGTGGGGACC AGGAATCCAG TGGGGCAGGC TCTGCTGGGG AAGAGGAGGT GGAGGAAAGC
    CAGGTAACCC CAACTCCAGC CATTGAGAGG GCAACGTGCT ACAACACCCC ACTCGGGTGC
    TGCTCTGATG GCAAGACTGC AGCTGCTGAT GCAGAAGGGA GCAACTGTCC GGCTACCAAA
    GTCTTCCAAG GAGTCCTCAT CTTGGAGGAG GTGGAAGGCC AGGAGCTGTT CTACACACCT
    GAGATGGCTG ACCCCAAGTC AGAGCTCTTC GGGGAGACAG CCAGGAGCAT CGAGAGCGCA
    CTGGACGAGC TTTTCCGAAA TTCTGATGTT AAGAATGATT TCAAGAGCAT CCGTGTCCGA
    GACCTGGGGC AGAGCAGCGC TGTCCGTGTC ATTGTGGAGT CCCACTTTGA CCCAGCCACT
    TCCTACACAG CAGCTGACGT TCAGGCGGCC CTGCTGAAGC AGATCAGAGC CTCCAAGAAG
    AGGACCATCC TGGTGAAGAA GCCCCAGCAG GAGCATGTCA AATTCATGGA CTTCGACTGG
    ATCCCCAGGA TCTTCACCAC CACCATCACC ACCACCACGG CCACCACCAT GGCACCTGCC
    ACCACACGGA GGCACACCAC AGCCTCTGCT GCCACCACTG CCCACATCCT GCGCCAGGAC
    ACCGTGGGTC ACCCCAGTGC CAAGCTGGCA GCACCTGCAA GCACCAGGAG GCCTACATCC
    ACCCTGCCTA CCACCGCCCG GCGCAAACCC ACTCGCCAGC CCCCCAGCAC CACCAAGAAG
    CCCTCACGAC CCTGCGACTC ACACCCCTGC CTGCATGGTG GCACCTGTGA GGACGATGGC
    AAGGAGTTCA CCTGCAGCTG CCCAGCAGGC AAAGGAGGAG CTGTGTGTGA GAAACCCATC
    AGGTACTTCA TCCCCAGCTT CGGTGGCAAG TCCTACCTGG CCTTCAAGAT GATGAAGGCG
    TACCACACCG TGCGCATCGC CATGGAGTTC AGGGCCACGG AGCTCAGCGG GCTGCTGCTC
    TACAACGGGC AGAATCGTGG CAAGGACTTC ATCTCCCTGG CGCTGGTGGG CGGCTTCGTG
    GAGCTCAGGT TTAACACAGG CTCTGGCACG GGCGTCATCA CCAGCAAGGT GCGCGTGGAG
    CCTGGCAAGT GGCACCAGCT GGTGGTGAAC CGCAACCGCC GCAGCGGCAT GCTGGCTGTG
    GATGGGGAGC CCCACGTCAG TGGGGAGAGC CCCACGGGTA CCGATGGGCT CAACCTCGAC
    ACCGACCTCT TTGTTGGAGG AGCTCCCGAG GACCAAATGG CTGTAGTGGC AGAGCGTACT
    GCGGCTACGG TTGGCCTGAA GGGCTGCATC CGCCTCCTGG ATGTGAACAA CCAGATGTAC
    GACCTGAGGG AGAAAGGCAG CGATGTGCTG TATGGCAGTG GGGTGGGCGA GTGTGGCAAC
    GACCCCTGCC ACCCCAACCC CTGCCACCAT GGGGGCATCT GCCACGTCAA GGAGGCAGAG
    ATGTTCCACT GCGAGTGTCT CCACTCCTAC ACCGGTCCAA CATGTGCTGA TGAGAGGAAC
    CCATGCGATC CAACGCCGTG CCACATCTCT GCCACCTGCC TGGTGCTGCC GGAGGGAGGT
    GCCATGTGTG CCTGTCCCAT GGGACGGGAA GGAGAGTTCT GTGAGCGAGT GACGGAGCAG
    GACCACACCA TGCCCTTCCT CCCAGAGTTC AATGGCTTTT CCTACCTGGA GCTGAACGGG
    CTGCAGACCT TTGTTCCTGA CCTGCAGGAC AAAATGTCCA TGGAAGTGGT GTTCCTGGCC
    AAGAGTCCCA GTGGCATGAT CTTCTACAAT GGGCAGAAGA CAGATGGCAA AGGAGACTTT
    GTGTCTCTGG CTTTGCACGA CGGCTACCTG GAGTATCGAT ATGACCTGGG CAAAGGGGCA
    GCTGTGCTCA GGAGCAAAGA GCCAGTTCCC CTCAACACCT GGATAAGCGT TTTGTTGGAG
    AGGAGTGGGC GCAAAGGGGT AATGAGGATC AACAATGGCG AGAGGGTGAT GGGAGAGTCA
    CCGGTGCCTC ACGCCTTCCT CAACCTGAAG GAGCCATTCT ACGTGGGGGG AGCCCCCGAT
    TTCAGCAAGC TGGCTCGAGC TGCAGCCATC TCCACCAGCT TCTATGGAGC TGTGCAGAGG
    ATCTCCATCA AAGGGGTTCC CTTGCTGAAG GAGCAGCACA TCCGCAGCGC CGTTGAAATC
    TCCACTTTCC GTGCCCACCC CTGCACCCAA AAGCCCAACC CGTGCCAGAA TGGAGGCACC
    TGCAGCCCCC GGTTGGAGAG CTACGAGTGT GCCTGCCAGA GGGGCTTCTC TGGGGCTCAC
    TGCGAGAAAG TGATCATTGA GAAGGCAGCT GGAGATGCAG AGGCCATTGC ATTTGATGGT
    AGGACGTACA TGGAGTACCA CAATGCCGTG ACAAAGAGCG AGAAGGCTTT GCAATCCAAC
    CACTTTGAGC TGAGCATCAA AACAGAAGCA ACCCAGGGCC TGATCTTGTG GAGTGGGAAG
    GGGCTGGAGC GATCCGACTA CATCGCCCTC GCCATCGTGG ATGGCTTCGT GCAGATGATG
    TATGACCTGG GCTCCAAGCC AGTCGTCCTG CGGTCCACAG TCCCCATCAA CACCAACCAC
    TGGACCCACA TCAAAGCATA CAGAGTACAG AGAGAAGGCT CCCTCCAGGT CGGCAACGAA
    GCTCCCATCA CTGGCTCTTC ACCGCTGGGT GCCACACAGC TGGACACGGA TGGGGCCCTG
    TGGCTGGGGG GAATGGAGCG GCTCAGCGTG GCTCACAAGC TGCCCAAGGC TTACAGCACT
    GGCTTTATTG GCTGTATAAG GGACGTGATT GTCGACCGCC AAGAACTGCA TCTGGTGGAG
    GACGCTTTAA ACAACCCCAC GATATTACAC TGCTCAGCCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152331.1
    CDS170..6334
    Translation

    Target ORF information:

    RefSeq Version XM_015296845.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAC AAGCCCAATA GCTATTTTAC CCAGACCCCG
    CCGACCCCTC GAGATGCCTG CCGAGGGATG CTGTGTGGGT TCGGGGCGGT GTGCGAGCGC
    AGCCCCACTG ACCCCTCCCA AGCCTCCTGT GTCTGCAAGA AGACAGCTTG CCCTGTGGTG
    GTGGCTCCTG TCTGCGGCTC CGACTATTCC ACCTACAGCA ACGAGTGTGA GCTGGAGAAA
    GCCCAGTGCA ACCAGCAGAG GCGGATCAAG GTCATCAGCA AGGGACCATG TGGCTCCAAG
    GACCCCTGTG CAGAGGTGAC GTGCAGCTTT GGCAGCACGT GCGTCCGCTC TGCAGATGGC
    CAGACGGCCA GGTGTGTGTG CCCCGCATCC TGCAGCGGTG TGGCTGAGAG CATCGTCTGC
    GGCAGTGATG GCAAGGACTA CCGCAGTGAG TGCGACCTCA ACAAGCACGC CTGTGACAAG
    CAGGAGAATG TCTTCAAGAA GTTTGATGGA GCCTGCGACC CCTGTAAGGG CATCCTCAAC
    GACATGAACC GTGTGTGCCG GGTGAACCCC CGCACCCGCC GGGTGGAGCT GCTCTCCCGC
    CCCGAGAACT GTCCCTCAAA GAGGGAGCCA GTGTGCGGTG ACGATGGGGT GACGTATGCC
    AGTGAGTGTG TGATGGGGCG CACGGGGGCC ATCCGGGGGC TGGAGATCCA GAAGGTGCGC
    TCTGGGCAGT GCCAGCACCA GGACAAATGC AAGGATGAAT GCAAGTTCAA TGCTGTCTGC
    CTGAACCGCC GTGGCACGGC GCGCTGCTCC TGCGACCGCA TCACTTGTGA TGGCACCTAC
    CGGCCTGTCT GTGCCCGCGA CAGCCGCACC TACAGCAACG ACTGTGAGCG GCAGAAAGCC
    GAGTGCCACC AGAAAGCTGC CATCCCAGTG AAGCACAGCG GACCCTGTGA CCTGGGTACT
    CCCAGCCCCT GCCTGAGCGT GGAGTGCACT TTTGGGGCCA CCTGCGTGGT GAAGAACCGG
    GAGCCCGTGT GCGAGTGCCA GCAGGTGTGC CAGGGCCGCT ATGACCCCGT CTGCGGCAGC
    GACAACCGCA CCTACGGCAA CCCCTGCGAG CTCAACGCCA TGGCCTGCGT CCTCAAGAGA
    GAGATCAGGG TGAAGCACAA GGGGCCCTGC GACCGCTGTG GCAAGTGCCA GTTTGGGGCC
    ATCTGCGAGG CGGAGACGGG GCGGTGCGTG TGCCCCACGG AGTGCGTGCC CTCCTCCCAG
    CCTGTCTGTG GCACAGATGG CAACACCTAC GGCAGCGAGT GTGAGCTCCA CGTCCGAGCG
    TGCACGCAGC AGAAGAACAT TTTGGTGGCT GCCCAAGGCG ACTGCAAGTC CTGCGGCAGC
    ACGGTGTGCT CCTTCGGCAG CACGTGTGTG GGTGGGCAGT GCGTGTGTCC CCGCTGTGAG
    CAGCAGCCCC TGGCCCAGGT CTGTGGCACC GATGGCCTCA CCTATGACAA CCGCTGCGAG
    CTCCGAGCAG CCTCCTGCCA ACAGCAGAAG AGCATCGAGG TGGCCAAGAT GGGGCCGTGT
    GAGGACGAAT GTGGCTCAGG GGGCTCAGGC TCTGGTGATG GGAGTGAGTG TGAGCAGGAC
    CGGTGCCGGC ACTACGGGGG CTGGTGGGAT GAGGATGCAG AGGACGACCG CTGTGTGTGT
    GACTTCACCT GCCTGGCGGT GCCACGCAGC CCGGTCTGTG GCTCTGACGA TGTGACCTAC
    GCCAATGAGT GTGAGCTGAA GAAGACGAGG TGTGAAAAAC GCCAGGACCT CTATGTCACC
    AGCCAAGGAG CCTGCCGTGC CCTCACCACG ACCCCACCGC CTCTCCCAGT TGTGCACTGC
    AGTCAGACCA TCTATGGGTG CTGCCCGGAC AACATGACCC TGGCGCTCGG AGTGGGTGCA
    GCCGGGTGCC CAAGCACCTG CCAGTGCAAC CCCTATGGCT CCTATGGAGG AACCTGCGAC
    CCTGCCACGG GGCAGTGCTC CTGCAAGCCA GGTGTTGGGG GGCTCAAGTG TGACCGCTGT
    GAGCCTGGCT TCTGGAACTT CCGTGGCATC GTCACCGACA GCAAGAGCGG CTGCACGCCC
    TGTAACTGCG ACCCGGTGGG CTCAGTGCGT GATGACTGCG AGCAGATGAC TGGGCTCTGC
    TCCTGCAAGA CTGGGATCAC TGGCATGAAG TGCAACCAGT GTCCCAACGG CAGCAAGATG
    GGCATGGCTG GCTGTGAAAA AGACCCATCA GCTCCAAAAT CCTGTGAGGA AATGAGCTGC
    GAGTTCGGGG CTACGTGTGT GGAGGTCAAC GGCTTTGCCC ACTGCGAGTG CCCGTCCCCA
    CTCTGCTCAG AGGCCAACAT GACCAAGGTG TGTGGCTCTG ATGGTGTTAC CTATGGGGAC
    CAGTGCCAGC TGAAGACCAT TGCGTGCCGG CAGGGACAGC TCATCACAGT GAAACACGTG
    GGGCAATGCC ACGAGTCTAT AACTCACACG AGCCATACGA TGCCACCCAC CCCTCTGCCC
    ACCTTGCCCT TGGACAAGCT CATCGTACCC CCACCACTGC AGCTGACCAC CCAGGCTCCA
    GAGCCCACTG AGCTGGCCAC CACCTCCCTG CTGATGGAAG CCAGCCCCAC TACAAGAAGT
    CACCCTACAA CAAGGCGTGT CACAACGACC CGACCTGTCA CCACACCATG GATGACCCAT
    GGAGTGCTGA AGACCACCGT CCGCCCACTC TCCACCTCTC CAGTGGTCCT GGCCACCACT
    CAGCCTCCCT ACGCTGAATC GGGCAGTGCA GAAGGCAGTG GGGACCAGGA GATGAGCATC
    AGTGGGGACC AGGAATCCAG TGGGGCAGGC TCTGCTGGGG AAGAGGAGGT GGAGGAAAGC
    CAGGTAACCC CAACTCCAGC CATTGAGAGG GCAACGTGCT ACAACACCCC ACTCGGGTGC
    TGCTCTGATG GCAAGACTGC AGCTGCTGAT GCAGAAGGGA GCAACTGTCC GGCTACCAAA
    GTCTTCCAAG GAGTCCTCAT CTTGGAGGAG GTGGAAGGCC AGGAGCTGTT CTACACACCT
    GAGATGGCTG ACCCCAAGTC AGAGCTCTTC GGGGAGACAG CCAGGAGCAT CGAGAGCGCA
    CTGGACGAGC TTTTCCGAAA TTCTGATGTT AAGAATGATT TCAAGAGCAT CCGTGTCCGA
    GACCTGGGGC AGAGCAGCGC TGTCCGTGTC ATTGTGGAGT CCCACTTTGA CCCAGCCACT
    TCCTACACAG CAGCTGACGT TCAGGCGGCC CTGCTGAAGC AGATCAGAGC CTCCAAGAAG
    AGGACCATCC TGGTGAAGAA GCCCCAGCAG GAGCATGTCA AATTCATGGA CTTCGACTGG
    ATCCCCAGGA TCTTCACCAC CACCATCACC ACCACCACGG CCACCACCAT GGCACCTGCC
    ACCACACGGA GGCACACCAC AGCCTCTGCT GCCACCACTG CCCACATCCT GCGCCAGGAC
    ACCGTGGGTC ACCCCAGTGC CAAGCTGGCA GCACCTGCAA GCACCAGGAG GCCTACATCC
    ACCCTGCCTA CCACCGCCCG GCGCAAACCC ACTCGCCAGC CCCCCAGCAC CACCAAGAAG
    CCCTCACGAC CCTGCGACTC ACACCCCTGC CTGCATGGTG GCACCTGTGA GGACGATGGC
    AAGGAGTTCA CCTGCAGCTG CCCAGCAGGC AAAGGAGGAG CTGTGTGTGA GAAACCCATC
    AGGTACTTCA TCCCCAGCTT CGGTGGCAAG TCCTACCTGG CCTTCAAGAT GATGAAGGCG
    TACCACACCG TGCGCATCGC CATGGAGTTC AGGGCCACGG AGCTCAGCGG GCTGCTGCTC
    TACAACGGGC AGAATCGTGG CAAGGACTTC ATCTCCCTGG CGCTGGTGGG CGGCTTCGTG
    GAGCTCAGGT TTAACACAGG CTCTGGCACG GGCGTCATCA CCAGCAAGGT GCGCGTGGAG
    CCTGGCAAGT GGCACCAGCT GGTGGTGAAC CGCAACCGCC GCAGCGGCAT GCTGGCTGTG
    GATGGGGAGC CCCACGTCAG TGGGGAGAGC CCCACGGGTA CCGATGGGCT CAACCTCGAC
    ACCGACCTCT TTGTTGGAGG AGCTCCCGAG GACCAAATGG CTGTAGTGGC AGAGCGTACT
    GCGGCTACGG TTGGCCTGAA GGGCTGCATC CGCCTCCTGG ATGTGAACAA CCAGATGTAC
    GACCTGAGGG AGAAAGGCAG CGATGTGCTG TATGGCAGTG GGGTGGGCGA GTGTGGCAAC
    GACCCCTGCC ACCCCAACCC CTGCCACCAT GGGGGCATCT GCCACGTCAA GGAGGCAGAG
    ATGTTCCACT GCGAGTGTCT CCACTCCTAC ACCGGTCCAA CATGTGCTGA TGAGAGGAAC
    CCATGCGATC CAACGCCGTG CCACATCTCT GCCACCTGCC TGGTGCTGCC GGAGGGAGGT
    GCCATGTGTG CCTGTCCCAT GGGACGGGAA GGAGAGTTCT GTGAGCGAGT GACGGAGCAG
    GACCACACCA TGCCCTTCCT CCCAGAGTTC AATGGCTTTT CCTACCTGGA GCTGAACGGG
    CTGCAGACCT TTGTTCCTGA CCTGCAGGAC AAAATGTCCA TGGAAGTGGT GTTCCTGGCC
    AAGAGTCCCA GTGGCATGAT CTTCTACAAT GGGCAGAAGA CAGATGGCAA AGGAGACTTT
    GTGTCTCTGG CTTTGCACGA CGGCTACCTG GAGTATCGAT ATGACCTGGG CAAAGGGGCA
    GCTGTGCTCA GGAGCAAAGA GCCAGTTCCC CTCAACACCT GGATAAGCGT TTTGTTGGAG
    AGGAGTGGGC GCAAAGGGGT AATGAGGATC AACAATGGCG AGAGGGTGAT GGGAGAGTCA
    CCGGTGCCTC ACGCCTTCCT CAACCTGAAG GAGCCATTCT ACGTGGGGGG AGCCCCCGAT
    TTCAGCAAGC TGGCTCGAGC TGCAGCCATC TCCACCAGCT TCTATGGAGC TGTGCAGAGG
    ATCTCCATCA AAGGGGTTCC CTTGCTGAAG GAGCAGCACA TCCGCAGCGC CGTTGAAATC
    TCCACTTTCC GTGCCCACCC CTGCACCCAA AAGCCCAACC CGTGCCAGAA TGGAGGCACC
    TGCAGCCCCC GGTTGGAGAG CTACGAGTGT GCCTGCCAGA GGGGCTTCTC TGGGGCTCAC
    TGCGAGAAAG TGATCATTGA GAAGGCAGCT GGAGATGCAG AGGCCATTGC ATTTGATGGT
    AGGACGTACA TGGAGTACCA CAATGCCGTG ACAAAGAGCG AGAAGGCTTT GCAATCCAAC
    CACTTTGAGC TGAGCATCAA AACAGAAGCA ACCCAGGGCC TGATCTTGTG GAGTGGGAAG
    GGGCTGGAGC GATCCGACTA CATCGCCCTC GCCATCGTGG ATGGCTTCGT GCAGATGATG
    TATGACCTGG GCTCCAAGCC AGTCGTCCTG CGGTCCACAG TCCCCATCAA CACCAACCAC
    TGGACCCACA TCAAAGCATA CAGAGTACAG AGAGAAGGCT CCCTCCAGGT CGGCAACGAA
    GCTCCCATCA CTGGCTCTTC ACCGCTGGGT GCCACACAGC TGGACACGGA TGGGGCCCTG
    TGGCTGGGGG GAATGGAGCG GCTCAGCGTG GCTCACAAGC TGCCCAAGGC TTACAGCACT
    GGCTTTATTG GCTGTATAAG GGACGTGATT GTCGACCGCC AAGAACTGCA TCTGGTGGAG
    GACGCTTTAA ACAACCCCAC GATATTACAC TGCTCAGCCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa45018
    Clone ID Related Accession (Same CDS sequence) XM_015296845.1 , XM_015296845.2
    Accession Version XM_015296845.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product agrin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAC AAGCCCAATA GCTATTTTAC CCAGACCCCG
    CCGACCCCTC GAGATGCCTG CCGAGGGATG CTGTGTGGGT TCGGGGCGGT GTGCGAGCGC
    AGCCCCACTG ACCCCTCCCA AGCCTCCTGT GTCTGCAAGA AGACAGCTTG CCCTGTGGTG
    GTGGCTCCTG TCTGCGGCTC CGACTATTCC ACCTACAGCA ACGAGTGTGA GCTGGAGAAA
    GCCCAGTGCA ACCAGCAGAG GCGGATCAAG GTCATCAGCA AGGGACCATG TGGCTCCAAG
    GACCCCTGTG CAGAGGTGAC GTGCAGCTTT GGCAGCACGT GCGTCCGCTC TGCAGATGGC
    CAGACGGCCA GGTGTGTGTG CCCCGCATCC TGCAGCGGTG TGGCTGAGAG CATCGTCTGC
    GGCAGTGATG GCAAGGACTA CCGCAGTGAG TGCGACCTCA ACAAGCACGC CTGTGACAAG
    CAGGAGAATG TCTTCAAGAA GTTTGATGGA GCCTGCGACC CCTGTAAGGG CATCCTCAAC
    GACATGAACC GTGTGTGCCG GGTGAACCCC CGCACCCGCC GGGTGGAGCT GCTCTCCCGC
    CCCGAGAACT GTCCCTCAAA GAGGGAGCCA GTGTGCGGTG ACGATGGGGT GACGTATGCC
    AGTGAGTGTG TGATGGGGCG CACGGGGGCC ATCCGGGGGC TGGAGATCCA GAAGGTGCGC
    TCTGGGCAGT GCCAGCACCA GGACAAATGC AAGGATGAAT GCAAGTTCAA TGCTGTCTGC
    CTGAACCGCC GTGGCACGGC GCGCTGCTCC TGCGACCGCA TCACTTGTGA TGGCACCTAC
    CGGCCTGTCT GTGCCCGCGA CAGCCGCACC TACAGCAACG ACTGTGAGCG GCAGAAAGCC
    GAGTGCCACC AGAAAGCTGC CATCCCAGTG AAGCACAGCG GACCCTGTGA CCTGGGTACT
    CCCAGCCCCT GCCTGAGCGT GGAGTGCACT TTTGGGGCCA CCTGCGTGGT GAAGAACCGG
    GAGCCCGTGT GCGAGTGCCA GCAGGTGTGC CAGGGCCGCT ATGACCCCGT CTGCGGCAGC
    GACAACCGCA CCTACGGCAA CCCCTGCGAG CTCAACGCCA TGGCCTGCGT CCTCAAGAGA
    GAGATCAGGG TGAAGCACAA GGGGCCCTGC GACCGCTGTG GCAAGTGCCA GTTTGGGGCC
    ATCTGCGAGG CGGAGACGGG GCGGTGCGTG TGCCCCACGG AGTGCGTGCC CTCCTCCCAG
    CCTGTCTGTG GCACAGATGG CAACACCTAC GGCAGCGAGT GTGAGCTCCA CGTCCGAGCG
    TGCACGCAGC AGAAGAACAT TTTGGTGGCT GCCCAAGGCG ACTGCAAGTC CTGCGGCAGC
    ACGGTGTGCT CCTTCGGCAG CACGTGTGTG GGTGGGCAGT GCGTGTGTCC CCGCTGTGAG
    CAGCAGCCCC TGGCCCAGGT CTGTGGCACC GATGGCCTCA CCTATGACAA CCGCTGCGAG
    CTCCGAGCAG CCTCCTGCCA ACAGCAGAAG AGCATCGAGG TGGCCAAGAT GGGGCCGTGT
    GAGGACGAAT GTGGCTCAGG GGGCTCAGGC TCTGGTGATG GGAGTGAGTG TGAGCAGGAC
    CGGTGCCGGC ACTACGGGGG CTGGTGGGAT GAGGATGCAG AGGACGACCG CTGTGTGTGT
    GACTTCACCT GCCTGGCGGT GCCACGCAGC CCGGTCTGTG GCTCTGACGA TGTGACCTAC
    GCCAATGAGT GTGAGCTGAA GAAGACGAGG TGTGAAAAAC GCCAGGACCT CTATGTCACC
    AGCCAAGGAG CCTGCCGTGC CCTCACCACG ACCCCACCGC CTCTCCCAGT TGTGCACTGC
    AGTCAGACCA TCTATGGGTG CTGCCCGGAC AACATGACCC TGGCGCTCGG AGTGGGTGCA
    GCCGGGTGCC CAAGCACCTG CCAGTGCAAC CCCTATGGCT CCTATGGAGG AACCTGCGAC
    CCTGCCACGG GGCAGTGCTC CTGCAAGCCA GGTGTTGGGG GGCTCAAGTG TGACCGCTGT
    GAGCCTGGCT TCTGGAACTT CCGTGGCATC GTCACCGACA GCAAGAGCGG CTGCACGCCC
    TGTAACTGCG ACCCGGTGGG CTCAGTGCGT GATGACTGCG AGCAGATGAC TGGGCTCTGC
    TCCTGCAAGA CTGGGATCAC TGGCATGAAG TGCAACCAGT GTCCCAACGG CAGCAAGATG
    GGCATGGCTG GCTGTGAAAA AGACCCATCA GCTCCAAAAT CCTGTGAGGA AATGAGCTGC
    GAGTTCGGGG CTACGTGTGT GGAGGTCAAC GGCTTTGCCC ACTGCGAGTG CCCGTCCCCA
    CTCTGCTCAG AGGCCAACAT GACCAAGGTG TGTGGCTCTG ATGGTGTTAC CTATGGGGAC
    CAGTGCCAGC TGAAGACCAT TGCGTGCCGG CAGGGACAGC TCATCACAGT GAAACACGTG
    GGGCAATGCC ACGAGTCTAT AACTCACACG AGCCATACGA TGCCACCCAC CCCTCTGCCC
    ACCTTGCCCT TGGACAAGCT CATCGTACCC CCACCACTGC AGCTGACCAC CCAGGCTCCA
    GAGCCCACTG AGCTGGCCAC CACCTCCCTG CTGATGGAAG CCAGCCCCAC TACAAGAAGT
    CACCCTACAA CAAGGCGTGT CACAACGACC CGACCTGTCA CCACACCATG GATGACCCAT
    GGAGTGCTGA AGACCACCGT CCGCCCACTC TCCACCTCTC CAGTGGTCCT GGCCACCACT
    CAGCCTCCCT ACGCTGAATC GGGCAGTGCA GAAGGCAGTG GGGACCAGGA GATGAGCATC
    AGTGGGGACC AGGAATCCAG TGGGGCAGGC TCTGCTGGGG AAGAGGAGGT GGAGGAAAGC
    CAGGTAACCC CAACTCCAGC CATTGAGAGG GCAACGTGCT ACAACACCCC ACTCGGGTGC
    TGCTCTGATG GCAAGACTGC AGCTGCTGAT GCAGAAGGGA GCAACTGTCC GGCTACCAAA
    GTCTTCCAAG GAGTCCTCAT CTTGGAGGAG GTGGAAGGCC AGGAGCTGTT CTACACACCT
    GAGATGGCTG ACCCCAAGTC AGAGCTCTTC GGGGAGACAG CCAGGAGCAT CGAGAGCGCA
    CTGGACGAGC TTTTCCGAAA TTCTGATGTT AAGAATGATT TCAAGAGCAT CCGTGTCCGA
    GACCTGGGGC AGAGCAGCGC TGTCCGTGTC ATTGTGGAGT CCCACTTTGA CCCAGCCACT
    TCCTACACAG CAGCTGACGT TCAGGCGGCC CTGCTGAAGC AGATCAGAGC CTCCAAGAAG
    AGGACCATCC TGGTGAAGAA GCCCCAGCAG GAGCATGTCA AATTCATGGA CTTCGACTGG
    ATCCCCAGGA TCTTCACCAC CACCATCACC ACCACCACGG CCACCACCAT GGCACCTGCC
    ACCACACGGA GGCACACCAC AGCCTCTGCT GCCACCACTG CCCACATCCT GCGCCAGGAC
    ACCGTGGGTC ACCCCAGTGC CAAGCTGGCA GCACCTGCAA GCACCAGGAG GCCTACATCC
    ACCCTGCCTA CCACCGCCCG GCGCAAACCC ACTCGCCAGC CCCCCAGCAC CACCAAGAAG
    CCCTCACGAC CCTGCGACTC ACACCCCTGC CTGCATGGTG GCACCTGTGA GGACGATGGC
    AAGGAGTTCA CCTGCAGCTG CCCAGCAGGC AAAGGAGGAG CTGTGTGTGA GAAACCCATC
    AGGTACTTCA TCCCCAGCTT CGGTGGCAAG TCCTACCTGG CCTTCAAGAT GATGAAGGCG
    TACCACACCG TGCGCATCGC CATGGAGTTC AGGGCCACGG AGCTCAGCGG GCTGCTGCTC
    TACAACGGGC AGAATCGTGG CAAGGACTTC ATCTCCCTGG CGCTGGTGGG CGGCTTCGTG
    GAGCTCAGGT TTAACACAGG CTCTGGCACG GGCGTCATCA CCAGCAAGGT GCGCGTGGAG
    CCTGGCAAGT GGCACCAGCT GGTGGTGAAC CGCAACCGCC GCAGCGGCAT GCTGGCTGTG
    GATGGGGAGC CCCACGTCAG TGGGGAGAGC CCCACGGGTA CCGATGGGCT CAACCTCGAC
    ACCGACCTCT TTGTTGGAGG AGCTCCCGAG GACCAAATGG CTGTAGTGGC AGAGCGTACT
    GCGGCTACGG TTGGCCTGAA GGGCTGCATC CGCCTCCTGG ATGTGAACAA CCAGATGTAC
    GACCTGAGGG AGAAAGGCAG CGATGTGCTG TATGGCAGTG GGGTGGGCGA GTGTGGCAAC
    GACCCCTGCC ACCCCAACCC CTGCCACCAT GGGGGCATCT GCCACGTCAA GGAGGCAGAG
    ATGTTCCACT GCGAGTGTCT CCACTCCTAC ACCGGTCCAA CATGTGCTGA TGAGAGGAAC
    CCATGCGATC CAACGCCGTG CCACATCTCT GCCACCTGCC TGGTGCTGCC GGAGGGAGGT
    GCCATGTGTG CCTGTCCCAT GGGACGGGAA GGAGAGTTCT GTGAGCGAGT GACGGAGCAG
    GACCACACCA TGCCCTTCCT CCCAGAGTTC AATGGCTTTT CCTACCTGGA GCTGAACGGG
    CTGCAGACCT TTGTTCCTGA CCTGCAGGAC AAAATGTCCA TGGAAGTGGT GTTCCTGGCC
    AAGAGTCCCA GTGGCATGAT CTTCTACAAT GGGCAGAAGA CAGATGGCAA AGGAGACTTT
    GTGTCTCTGG CTTTGCACGA CGGCTACCTG GAGTATCGAT ATGACCTGGG CAAAGGGGCA
    GCTGTGCTCA GGAGCAAAGA GCCAGTTCCC CTCAACACCT GGATAAGCGT TTTGTTGGAG
    AGGAGTGGGC GCAAAGGGGT AATGAGGATC AACAATGGCG AGAGGGTGAT GGGAGAGTCA
    CCGGTGCCTC ACGCCTTCCT CAACCTGAAG GAGCCATTCT ACGTGGGGGG AGCCCCCGAT
    TTCAGCAAGC TGGCTCGAGC TGCAGCCATC TCCACCAGCT TCTATGGAGC TGTGCAGAGG
    ATCTCCATCA AAGGGGTTCC CTTGCTGAAG GAGCAGCACA TCCGCAGCGC CGTTGAAATC
    TCCACTTTCC GTGCCCACCC CTGCACCCAA AAGCCCAACC CGTGCCAGAA TGGAGGCACC
    TGCAGCCCCC GGTTGGAGAG CTACGAGTGT GCCTGCCAGA GGGGCTTCTC TGGGGCTCAC
    TGCGAGAAAG TGATCATTGA GAAGGCAGCT GGAGATGCAG AGGCCATTGC ATTTGATGGT
    AGGACGTACA TGGAGTACCA CAATGCCGTG ACAAAGAGCG AGAAGGCTTT GCAATCCAAC
    CACTTTGAGC TGAGCATCAA AACAGAAGCA ACCCAGGGCC TGATCTTGTG GAGTGGGAAG
    GGGCTGGAGC GATCCGACTA CATCGCCCTC GCCATCGTGG ATGGCTTCGT GCAGATGATG
    TATGACCTGG GCTCCAAGCC AGTCGTCCTG CGGTCCACAG TCCCCATCAA CACCAACCAC
    TGGACCCACA TCAAAGCATA CAGAGTACAG AGAGAAGGCT CCCTCCAGGT CGGCAACGAA
    GCTCCCATCA CTGGCTCTTC ACCGCTGGGT GCCACACAGC TGGACACGGA TGGGGCCCTG
    TGGCTGGGGG GAATGGAGCG GCTCAGCGTG GCTCACAAGC TGCCCAAGGC TTACAGCACT
    GGCTTTATTG GCTGTATAAG GGACGTGATT GTCGACCGCC AAGAACTGCA TCTGGTGGAG
    GACGCTTTAA ACAACCCCAC GATATTACAC TGCTCAGCCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015152331.1
    CDS186..6350
    Translation

    Target ORF information:

    RefSeq Version XM_015296845.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015296845.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAC AAGCCCAATA GCTATTTTAC CCAGACCCCG
    CCGACCCCTC GAGATGCCTG CCGAGGGATG CTGTGTGGGT TCGGGGCGGT GTGCGAGCGC
    AGCCCCACTG ACCCCTCCCA AGCCTCCTGT GTCTGCAAGA AGACAGCTTG CCCTGTGGTG
    GTGGCTCCTG TCTGCGGCTC CGACTATTCC ACCTACAGCA ACGAGTGTGA GCTGGAGAAA
    GCCCAGTGCA ACCAGCAGAG GCGGATCAAG GTCATCAGCA AGGGACCATG TGGCTCCAAG
    GACCCCTGTG CAGAGGTGAC GTGCAGCTTT GGCAGCACGT GCGTCCGCTC TGCAGATGGC
    CAGACGGCCA GGTGTGTGTG CCCCGCATCC TGCAGCGGTG TGGCTGAGAG CATCGTCTGC
    GGCAGTGATG GCAAGGACTA CCGCAGTGAG TGCGACCTCA ACAAGCACGC CTGTGACAAG
    CAGGAGAATG TCTTCAAGAA GTTTGATGGA GCCTGCGACC CCTGTAAGGG CATCCTCAAC
    GACATGAACC GTGTGTGCCG GGTGAACCCC CGCACCCGCC GGGTGGAGCT GCTCTCCCGC
    CCCGAGAACT GTCCCTCAAA GAGGGAGCCA GTGTGCGGTG ACGATGGGGT GACGTATGCC
    AGTGAGTGTG TGATGGGGCG CACGGGGGCC ATCCGGGGGC TGGAGATCCA GAAGGTGCGC
    TCTGGGCAGT GCCAGCACCA GGACAAATGC AAGGATGAAT GCAAGTTCAA TGCTGTCTGC
    CTGAACCGCC GTGGCACGGC GCGCTGCTCC TGCGACCGCA TCACTTGTGA TGGCACCTAC
    CGGCCTGTCT GTGCCCGCGA CAGCCGCACC TACAGCAACG ACTGTGAGCG GCAGAAAGCC
    GAGTGCCACC AGAAAGCTGC CATCCCAGTG AAGCACAGCG GACCCTGTGA CCTGGGTACT
    CCCAGCCCCT GCCTGAGCGT GGAGTGCACT TTTGGGGCCA CCTGCGTGGT GAAGAACCGG
    GAGCCCGTGT GCGAGTGCCA GCAGGTGTGC CAGGGCCGCT ATGACCCCGT CTGCGGCAGC
    GACAACCGCA CCTACGGCAA CCCCTGCGAG CTCAACGCCA TGGCCTGCGT CCTCAAGAGA
    GAGATCAGGG TGAAGCACAA GGGGCCCTGC GACCGCTGTG GCAAGTGCCA GTTTGGGGCC
    ATCTGCGAGG CGGAGACGGG GCGGTGCGTG TGCCCCACGG AGTGCGTGCC CTCCTCCCAG
    CCTGTCTGTG GCACAGATGG CAACACCTAC GGCAGCGAGT GTGAGCTCCA CGTCCGAGCG
    TGCACGCAGC AGAAGAACAT TTTGGTGGCT GCCCAAGGCG ACTGCAAGTC CTGCGGCAGC
    ACGGTGTGCT CCTTCGGCAG CACGTGTGTG GGTGGGCAGT GCGTGTGTCC CCGCTGTGAG
    CAGCAGCCCC TGGCCCAGGT CTGTGGCACC GATGGCCTCA CCTATGACAA CCGCTGCGAG
    CTCCGAGCAG CCTCCTGCCA ACAGCAGAAG AGCATCGAGG TGGCCAAGAT GGGGCCGTGT
    GAGGACGAAT GTGGCTCAGG GGGCTCAGGC TCTGGTGATG GGAGTGAGTG TGAGCAGGAC
    CGGTGCCGGC ACTACGGGGG CTGGTGGGAT GAGGATGCAG AGGACGACCG CTGTGTGTGT
    GACTTCACCT GCCTGGCGGT GCCACGCAGC CCGGTCTGTG GCTCTGACGA TGTGACCTAC
    GCCAATGAGT GTGAGCTGAA GAAGACGAGG TGTGAAAAAC GCCAGGACCT CTATGTCACC
    AGCCAAGGAG CCTGCCGTGC CCTCACCACG ACCCCACCGC CTCTCCCAGT TGTGCACTGC
    AGTCAGACCA TCTATGGGTG CTGCCCGGAC AACATGACCC TGGCGCTCGG AGTGGGTGCA
    GCCGGGTGCC CAAGCACCTG CCAGTGCAAC CCCTATGGCT CCTATGGAGG AACCTGCGAC
    CCTGCCACGG GGCAGTGCTC CTGCAAGCCA GGTGTTGGGG GGCTCAAGTG TGACCGCTGT
    GAGCCTGGCT TCTGGAACTT CCGTGGCATC GTCACCGACA GCAAGAGCGG CTGCACGCCC
    TGTAACTGCG ACCCGGTGGG CTCAGTGCGT GATGACTGCG AGCAGATGAC TGGGCTCTGC
    TCCTGCAAGA CTGGGATCAC TGGCATGAAG TGCAACCAGT GTCCCAACGG CAGCAAGATG
    GGCATGGCTG GCTGTGAAAA AGACCCATCA GCTCCAAAAT CCTGTGAGGA AATGAGCTGC
    GAGTTCGGGG CTACGTGTGT GGAGGTCAAC GGCTTTGCCC ACTGCGAGTG CCCGTCCCCA
    CTCTGCTCAG AGGCCAACAT GACCAAGGTG TGTGGCTCTG ATGGTGTTAC CTATGGGGAC
    CAGTGCCAGC TGAAGACCAT TGCGTGCCGG CAGGGACAGC TCATCACAGT GAAACACGTG
    GGGCAATGCC ACGAGTCTAT AACTCACACG AGCCATACGA TGCCACCCAC CCCTCTGCCC
    ACCTTGCCCT TGGACAAGCT CATCGTACCC CCACCACTGC AGCTGACCAC CCAGGCTCCA
    GAGCCCACTG AGCTGGCCAC CACCTCCCTG CTGATGGAAG CCAGCCCCAC TACAAGAAGT
    CACCCTACAA CAAGGCGTGT CACAACGACC CGACCTGTCA CCACACCATG GATGACCCAT
    GGAGTGCTGA AGACCACCGT CCGCCCACTC TCCACCTCTC CAGTGGTCCT GGCCACCACT
    CAGCCTCCCT ACGCTGAATC GGGCAGTGCA GAAGGCAGTG GGGACCAGGA GATGAGCATC
    AGTGGGGACC AGGAATCCAG TGGGGCAGGC TCTGCTGGGG AAGAGGAGGT GGAGGAAAGC
    CAGGTAACCC CAACTCCAGC CATTGAGAGG GCAACGTGCT ACAACACCCC ACTCGGGTGC
    TGCTCTGATG GCAAGACTGC AGCTGCTGAT GCAGAAGGGA GCAACTGTCC GGCTACCAAA
    GTCTTCCAAG GAGTCCTCAT CTTGGAGGAG GTGGAAGGCC AGGAGCTGTT CTACACACCT
    GAGATGGCTG ACCCCAAGTC AGAGCTCTTC GGGGAGACAG CCAGGAGCAT CGAGAGCGCA
    CTGGACGAGC TTTTCCGAAA TTCTGATGTT AAGAATGATT TCAAGAGCAT CCGTGTCCGA
    GACCTGGGGC AGAGCAGCGC TGTCCGTGTC ATTGTGGAGT CCCACTTTGA CCCAGCCACT
    TCCTACACAG CAGCTGACGT TCAGGCGGCC CTGCTGAAGC AGATCAGAGC CTCCAAGAAG
    AGGACCATCC TGGTGAAGAA GCCCCAGCAG GAGCATGTCA AATTCATGGA CTTCGACTGG
    ATCCCCAGGA TCTTCACCAC CACCATCACC ACCACCACGG CCACCACCAT GGCACCTGCC
    ACCACACGGA GGCACACCAC AGCCTCTGCT GCCACCACTG CCCACATCCT GCGCCAGGAC
    ACCGTGGGTC ACCCCAGTGC CAAGCTGGCA GCACCTGCAA GCACCAGGAG GCCTACATCC
    ACCCTGCCTA CCACCGCCCG GCGCAAACCC ACTCGCCAGC CCCCCAGCAC CACCAAGAAG
    CCCTCACGAC CCTGCGACTC ACACCCCTGC CTGCATGGTG GCACCTGTGA GGACGATGGC
    AAGGAGTTCA CCTGCAGCTG CCCAGCAGGC AAAGGAGGAG CTGTGTGTGA GAAACCCATC
    AGGTACTTCA TCCCCAGCTT CGGTGGCAAG TCCTACCTGG CCTTCAAGAT GATGAAGGCG
    TACCACACCG TGCGCATCGC CATGGAGTTC AGGGCCACGG AGCTCAGCGG GCTGCTGCTC
    TACAACGGGC AGAATCGTGG CAAGGACTTC ATCTCCCTGG CGCTGGTGGG CGGCTTCGTG
    GAGCTCAGGT TTAACACAGG CTCTGGCACG GGCGTCATCA CCAGCAAGGT GCGCGTGGAG
    CCTGGCAAGT GGCACCAGCT GGTGGTGAAC CGCAACCGCC GCAGCGGCAT GCTGGCTGTG
    GATGGGGAGC CCCACGTCAG TGGGGAGAGC CCCACGGGTA CCGATGGGCT CAACCTCGAC
    ACCGACCTCT TTGTTGGAGG AGCTCCCGAG GACCAAATGG CTGTAGTGGC AGAGCGTACT
    GCGGCTACGG TTGGCCTGAA GGGCTGCATC CGCCTCCTGG ATGTGAACAA CCAGATGTAC
    GACCTGAGGG AGAAAGGCAG CGATGTGCTG TATGGCAGTG GGGTGGGCGA GTGTGGCAAC
    GACCCCTGCC ACCCCAACCC CTGCCACCAT GGGGGCATCT GCCACGTCAA GGAGGCAGAG
    ATGTTCCACT GCGAGTGTCT CCACTCCTAC ACCGGTCCAA CATGTGCTGA TGAGAGGAAC
    CCATGCGATC CAACGCCGTG CCACATCTCT GCCACCTGCC TGGTGCTGCC GGAGGGAGGT
    GCCATGTGTG CCTGTCCCAT GGGACGGGAA GGAGAGTTCT GTGAGCGAGT GACGGAGCAG
    GACCACACCA TGCCCTTCCT CCCAGAGTTC AATGGCTTTT CCTACCTGGA GCTGAACGGG
    CTGCAGACCT TTGTTCCTGA CCTGCAGGAC AAAATGTCCA TGGAAGTGGT GTTCCTGGCC
    AAGAGTCCCA GTGGCATGAT CTTCTACAAT GGGCAGAAGA CAGATGGCAA AGGAGACTTT
    GTGTCTCTGG CTTTGCACGA CGGCTACCTG GAGTATCGAT ATGACCTGGG CAAAGGGGCA
    GCTGTGCTCA GGAGCAAAGA GCCAGTTCCC CTCAACACCT GGATAAGCGT TTTGTTGGAG
    AGGAGTGGGC GCAAAGGGGT AATGAGGATC AACAATGGCG AGAGGGTGAT GGGAGAGTCA
    CCGGTGCCTC ACGCCTTCCT CAACCTGAAG GAGCCATTCT ACGTGGGGGG AGCCCCCGAT
    TTCAGCAAGC TGGCTCGAGC TGCAGCCATC TCCACCAGCT TCTATGGAGC TGTGCAGAGG
    ATCTCCATCA AAGGGGTTCC CTTGCTGAAG GAGCAGCACA TCCGCAGCGC CGTTGAAATC
    TCCACTTTCC GTGCCCACCC CTGCACCCAA AAGCCCAACC CGTGCCAGAA TGGAGGCACC
    TGCAGCCCCC GGTTGGAGAG CTACGAGTGT GCCTGCCAGA GGGGCTTCTC TGGGGCTCAC
    TGCGAGAAAG TGATCATTGA GAAGGCAGCT GGAGATGCAG AGGCCATTGC ATTTGATGGT
    AGGACGTACA TGGAGTACCA CAATGCCGTG ACAAAGAGCG AGAAGGCTTT GCAATCCAAC
    CACTTTGAGC TGAGCATCAA AACAGAAGCA ACCCAGGGCC TGATCTTGTG GAGTGGGAAG
    GGGCTGGAGC GATCCGACTA CATCGCCCTC GCCATCGTGG ATGGCTTCGT GCAGATGATG
    TATGACCTGG GCTCCAAGCC AGTCGTCCTG CGGTCCACAG TCCCCATCAA CACCAACCAC
    TGGACCCACA TCAAAGCATA CAGAGTACAG AGAGAAGGCT CCCTCCAGGT CGGCAACGAA
    GCTCCCATCA CTGGCTCTTC ACCGCTGGGT GCCACACAGC TGGACACGGA TGGGGCCCTG
    TGGCTGGGGG GAATGGAGCG GCTCAGCGTG GCTCACAAGC TGCCCAAGGC TTACAGCACT
    GGCTTTATTG GCTGTATAAG GGACGTGATT GTCGACCGCC AAGAACTGCA TCTGGTGGAG
    GACGCTTTAA ACAACCCCAC GATATTACAC TGCTCAGCCA AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa28431
    Clone ID Related Accession (Same CDS sequence) NM_205527.3
    Accession Version NM_205527.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Gallus gallus(chicken)
    Product agrin precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000197.1. On Feb 11, 2016 this sequence version replaced NM_205527.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GTCCCGACGC ATTGGACTAC CCTGCTGAGC CCAGCGAGAA GGCTTTGCAA
    TCCAACCACT TTGAGCTGAG CATCAAAACA GAAGCAACCC AGGGCCTGAT CTTGTGGAGT
    GGGAAGGGGC TGGAGCGATC CGACTACATC GCCCTCGCCA TCGTGGATGG CTTCGTGCAG
    ATGATGTATG ACCTGGGCTC CAAGCCAGTC GTCCTGCGGT CCACAGTCCC CATCAACACC
    AACCACTGGA CCCACATCAA AGCATACAGA GTACAGAGAG AAGGCTCCCT CCAGGTCGGC
    AACGAAGCTC CCATCACTGG CTCTTCACCG CTGGGTGCCA CACAGCTGGA CACGGATGGG
    GCCCTGTGGC TGGGGGGAAT GGAGCGGCTC AGCGTGGCTC ACAAGCTGCC CAAGGCTTAC
    AGCACTGGCT TTATTGGCTG TATAAGGGAC GTGATTGTCG ACCGCCAAGA ACTGCATCTG
    GTGGAGGACG CTTTAAACAA CCCCACGATA TTACACTGCT CAGCCAAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_990858.2
    CDS93..6323
    Translation

    Target ORF information:

    RefSeq Version NM_205527.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus agrin (AGRN), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_205527.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGGGGT CGGGGGCGGC CGCGACCCTG GCGCTGGGTT TGGCGCTGGG ATTGGCCCTG 
    GGAGGGTGGG CGAACTGCCC CGAACGGGAG CTGCAGCGCC GAGAGGAGGA GGCCAACGTG
    GTGCTCACCG GAACGGTGGA GGAGATCATG AACGTGGACC CCGTGCACCA CACCTACTCG
    TGCAAGGTGA GAGTGTGGCG TTACCTGAAA GGCAAAGACA TCGTCACCCA CGAGATCCTC
    TTGGACGGTG GGAACAAAGT GGTCATCGGT GGCTTCGGGG ACCCCTTAAT CTGTGACAAC
    CAAGTGTCGA CCGGGGACAC ACGGATATTC TTTGTCAACC CAGCCCCTCA GTACATGTGG
    CCTGCCCATC GCAACGAGCT GATGCTCAAC TCCAGCCTGA TGCGGATCAC GCTGCGCAAC
    CTGGAGGAGG TGGAGCACTG CGTGGAAGAA CATAGGAAGC TTCTTGCTGA CAAGCCCAAT
    AGCTATTTTA CCCAGACCCC GCCGACCCCT CGAGATGCCT GCCGAGGGAT GCTGTGTGGG
    TTCGGGGCGG TGTGCGAGCG CAGCCCCACT GACCCCTCCC AAGCCTCCTG TGTCTGCAAG
    AAGACAGCTT GCCCTGTGGT GGTGGCTCCT GTCTGCGGCT CCGACTATTC CACCTACAGC
    AACGAGTGTG AGCTGGAGAA AGCCCAGTGC AACCAGCAGA GGCGGATCAA GGTCATCAGC
    AAGGGACCAT GTGGCTCCAA GGACCCCTGT GCAGAGGTGA CGTGCAGCTT TGGCAGCACG
    TGCGTCCGCT CTGCAGATGG CCAGACGGCC AGGTGTGTGT GCCCCGCATC CTGCAGCGGT
    GTGGCTGAGA GCATCGTCTG CGGCAGTGAT GGCAAGGACT ACCGCAGTGA GTGCGACCTC
    AACAAGCACG CCTGTGACAA GCAGGAGAAT GTCTTCAAGA AGTTTGATGG AGCCTGCGAC
    CCCTGTAAGG GCATCCTCAA CGACATGAAC CGTGTGTGCC GGGTGAACCC CCGCACCCGC
    CGGGTGGAGC TGCTCTCCCG CCCCGAGAAC TGTCCCTCAA AGAGGGAGCC AGTGTGCGGT
    GACGATGGGG TGACGTATGC CAGTGAGTGT GTGATGGGGC GCACGGGGGC CATCCGGGGG
    CTGGAGATCC AGAAGGTGCG CTCTGGGCAG TGCCAGCACC AGGACAAATG CAAGGATGAA
    TGCAAGTTCA ATGCTGTCTG CCTGAACCGC CGTGGCACGG CGCGCTGCTC CTGCGACCGC
    ATCACTTGTG ATGGCACCTA CCGGCCTGTC TGTGCCCGCG ACAGCCGCAC CTACAGCAAC
    GACTGTGAGC GGCAGAAAGC CGAGTGCCAC CAGAAAGCTG CCATCCCAGT GAAGCACAGC
    GGACCCTGTG ACCTGGGTAC TCCCAGCCCC TGCCTGAGCG TGGAGTGCAC TTTTGGGGCC
    ACCTGCGTGG TGAAGAACCG GGAGCCCGTG TGCGAGTGCC AGCAGGTGTG CCAGGGCCGC
    TATGACCCCG TCTGCGGCAG CGACAACCGC ACCTACGGCA ACCCCTGCGA GCTCAACGCC
    ATGGCCTGCG TCCTCAAGAG AGAGATCAGG GTGAAGCACA AGGGGCCCTG CGACCGCTGT
    GGCAAGTGCC AGTTTGGGGC CATCTGCGAG GCGGAGACGG GGCGGTGCGT GTGCCCCACG
    GAGTGCGTGC CCTCCTCCCA GCCTGTCTGT GGCACAGATG GCAACACCTA CGGCAGCGAG
    TGTGAGCTCC ACGTCCGAGC GTGCACGCAG CAGAAGAACA TTTTGGTGGC TGCCCAAGGC
    GACTGCAAGT CCTGCGGCAG CACGGTGTGC TCCTTCGGCA GCACGTGTGT GGGTGGGCAG
    TGCGTGTGTC CCCGCTGTGA GCAGCAGCCC CTGGCCCAGG TCTGTGGCAC CGATGGCCTC
    ACCTATGACA ACCGCTGCGA GCTCCGAGCA GCCTCCTGCC AACAGCAGAA GAGCATCGAG
    GTGGCCAAGA TGGGGCCGTG TGAGGACGAA TGTGGCTCAG GGGGCTCAGG CTCTGGTGAT
    GGGAGTGAGT GTGAGCAGGA CCGGTGCCGG CACTACGGGG GCTGGTGGGA TGAGGATGCA
    GAGGACGACC GCTGTGTGTG TGACTTCACC TGCCTGGCGG TGCCACGCAG CCCGGTCTGT
    GGCTCTGACG ATGTGACCTA CGCCAATGAG TGTGAGCTGA AGAAGACGAG GTGTGAAAAA
    CGCCAGGACC TCTATGTCAC CAGCCAAGGA GCCTGCCGTG CCCTCACCAC GACCCCACCG
    CCTCTCCCAG TTGTGCACTG CAGTCAGACC ATCTATGGGT GCTGCCCGGA CAACATGACC
    CTGGCGCTCG GAGTGGGTGC AGCCGGGTGC CCAAGCACCT GCCAGTGCAA CCCCTATGGC
    TCCTATGGAG GAACCTGCGA CCCTGCCACG GGGCAGTGCT CCTGCAAGCC AGGTGTTGGG
    GGGCTCAAGT GTGACCGCTG TGAGCCTGGC TTCTGGAACT TCCGTGGCAT CGTCACCGAC
    AGCAAGAGCG GCTGCACGCC CTGTAACTGC GACCCGGTGG GCTCAGTGCG TGATGACTGC
    GAGCAGATGA CTGGGCTCTG CTCCTGCAAG ACTGGGATCA CTGGCATGAA GTGCAACCAG
    TGTCCCAACG GCAGCAAGAT GGGCATGGCT GGCTGTGAAA AAGACCCATC AGCTCCAAAA
    TCCTGTGAGG AAATGAGCTG CGAGTTCGGG GCTACGTGTG TGGAGGTCAA CGGCTTTGCC
    CACTGCGAGT GCCCGTCCCC ACTCTGCTCA GAGGCCAACA TGACCAAGGT GTGTGGCTCT
    GATGGTGTTA CCTATGGGGA CCAGTGCCAG CTGAAGACCA TTGCGTGCCG GCAGGGACAG
    CTCATCACAG TGAAACACGT GGGGCAATGC CACGAGTCTA TAACTCACAC GAGCCATACG
    ATGCCACCCA CCCCTCTGCC CACCTTGCCC TTGGACAAGC TCATCGTACC CCCACCACTG
    CAGCTGACCA CCCAGGCTCC AGAGCCCACT GAGCTGGCCA CCACCTCCCT GCTGATGGAA
    GCCAGCCCCA CTACAAGAAG TCACCCTACA ACAAGGCGTG TCACAACGAC CCGACCTGTC
    ACCACACCAT GGATGACCCA TGGAGTGCTG AAGACCACCG TCCGCCCACT CTCCACCTCT
    CCAGTGGTCC TGGCCACCAC TCAGCCTCCC TACGCTGAAT CGGGCAGTGC AGAAGGCAGT
    GGGGACCAGG AGATGAGCAT CAGTGGGGAC CAGGAATCCA GTGGGGCAGG CTCTGCTGGG
    GAAGAGGAGG TGGAGGAAAG CCAGGTAACC CCAACTCCAG CCATTGAGAG GGCAACGTGC
    TACAACACCC CACTCGGGTG CTGCTCTGAT GGCAAGACTG CAGCTGCTGA TGCAGAAGGG
    AGCAACTGTC CGGCTACCAA AGTCTTCCAA GGAGTCCTCA TCTTGGAGGA GGTGGAAGGC
    CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAGCTCTT CGGGGAGACA
    GCCAGGAGCA TCGAGAGCGC ACTGGACGAG CTTTTCCGAA ATTCTGATGT TAAGAATGAT
    TTCAAGAGCA TCCGTGTCCG AGACCTGGGG CAGAGCAGCG CTGTCCGTGT CATTGTGGAG
    TCCCACTTTG ACCCAGCCAC TTCCTACACA GCAGCTGACG TTCAGGCGGC CCTGCTGAAG
    CAGATCAGAG CCTCCAAGAA GAGGACCATC CTGGTGAAGA AGCCCCAGCA GGAGCATGTC
    AAATTCATGG ACTTCGACTG GATCCCCAGG ATCTTCACCA CCACCATCAC CACCACCACG
    GCCACCACCA TGGCACCTGC CACCACACGG AGGCACACCA CAGCCTCTGC TGCCACCACT
    GCCCACATCC TGCGCCAGGA CACCGTGGGT CACCCCAGTG CCAAGCTGGC AGCACCTGCA
    AGCACCAGGA GGCCTACATC CACCCTGCCT ACCACCGCCC GGCGCAAACC CACTCGCCAG
    CCCCCCAGCA CCACCAAGAA GCCCTCACGA CCCTGCGACT CACACCCCTG CCTGCATGGT
    GGCACCTGTG AGGACGATGG CAAGGAGTTC ACCTGCAGCT GCCCAGCAGG CAAAGGAGGA
    GCTGTGTGTG AGAAACCCAT CAGGTACTTC ATCCCCAGCT TCGGTGGCAA GTCCTACCTG
    GCCTTCAAGA TGATGAAGGC GTACCACACC GTGCGCATCG CCATGGAGTT CAGGGCCACG
    GAGCTCAGCG GGCTGCTGCT CTACAACGGG CAGAATCGTG GCAAGGACTT CATCTCCCTG
    GCGCTGGTGG GCGGCTTCGT GGAGCTCAGG TTTAACACAG GCTCTGGCAC GGGCGTCATC
    ACCAGCAAGG TGCGCGTGGA GCCTGGCAAG TGGCACCAGC TGGTGGTGAA CCGCAACCGC
    CGCAGCGGCA TGCTGGCTGT GGATGGGGAG CCCCACGTCA GTGGGGAGAG CCCCACGGGT
    ACCGATGGGC TCAACCTCGA CACCGACCTC TTTGTTGGAG GAGCTCCCGA GGACCAAATG
    GCTGTAGTGG CAGAGCGTAC TGCGGCTACG GTTGGCCTGA AGGGCTGCAT CCGCCTCCTG
    GATGTGAACA ACCAGATGTA CGACCTGAGG GAGAAAGGCA GCGATGTGCT GTATGGCAGT
    GGGGTGGGCG AGTGTGGCAA CGACCCCTGC CACCCCAACC CCTGCCACCA TGGGGGCATC
    TGCCACGTCA AGGAGGCAGA GATGTTCCAC TGCGAGTGTC TCCACTCCTA CACCGGTCCA
    ACATGTGCTG ATGAGAGGAA CCCATGCGAT CCAACGCCGT GCCACATCTC TGCCACCTGC
    CTGGTGCTGC CGGAGGGAGG TGCCATGTGT GCCTGTCCCA TGGGACGGGA AGGAGAGTTC
    TGTGAGCGAG TGACGGAGCA GGACCACACC ATGCCCTTCC TCCCAGAGTT CAATGGCTTT
    TCCTACCTGG AGCTGAACGG GCTGCAGACC TTTGTTCCTG ACCTGCAGGA CAAAATGTCC
    ATGGAAGTGG TGTTCCTGGC CAAGAGTCCC AGTGGCATGA TCTTCTACAA TGGGCAGAAG
    ACAGATGGCA AAGGAGACTT TGTGTCTCTG GCTTTGCACG ACGGCTACCT GGAGTATCGA
    TATGACCTGG GCAAAGGGGC AGCTGTGCTC AGGAGCAAAG AGCCAGTTCC CCTCAACACC
    TGGATAAGCG TTTTGTTGGA GAGGAGTGGG CGCAAAGGGG TAATGAGGAT CAACAATGGC
    GAGAGGGTGA TGGGAGAGTC ACCGAAATCC CGTAAGGTGC CTCACGCCTT CCTCAACCTG
    AAGGAGCCAT TCTACGTGGG GGGAGCCCCC GATTTCAGCA AGCTGGCTCG AGCTGCAGCC
    ATCTCCACCA GCTTCTATGG AGCTGTGCAG AGGATCTCCA TCAAAGGGGT TCCCTTGCTG
    AAGGAGCAGC ACATCCGCAG CGCCGTTGAA ATCTCCACTT TCCGTGCCCA CCCCTGCACC
    CAAAAGCCCA ACCCGTGCCA GAATGGAGGC ACCTGCAGCC CCCGGTTGGA GAGCTACGAG
    TGTGCCTGCC AGAGGGGCTT CTCTGGGGCT CACTGCGAGA AAGTGATCAT TGAGAAGGCA
    GCTGGAGATG CAGAGGCCAT TGCATTTGAT GGTAGGACGT ACATGGAGTA CCACAATGCC
    GTGACAAAGA GTCCCGACGC ATTGGACTAC CCTGCTGAGC CCAGCGAGAA GGCTTTGCAA
    TCCAACCACT TTGAGCTGAG CATCAAAACA GAAGCAACCC AGGGCCTGAT CTTGTGGAGT
    GGGAAGGGGC TGGAGCGATC CGACTACATC GCCCTCGCCA TCGTGGATGG CTTCGTGCAG
    ATGATGTATG ACCTGGGCTC CAAGCCAGTC GTCCTGCGGT CCACAGTCCC CATCAACACC
    AACCACTGGA CCCACATCAA AGCATACAGA GTACAGAGAG AAGGCTCCCT CCAGGTCGGC
    AACGAAGCTC CCATCACTGG CTCTTCACCG CTGGGTGCCA CACAGCTGGA CACGGATGGG
    GCCCTGTGGC TGGGGGGAAT GGAGCGGCTC AGCGTGGCTC ACAAGCTGCC CAAGGCTTAC
    AGCACTGGCT TTATTGGCTG TATAAGGGAC GTGATTGTCG ACCGCCAAGA ACTGCATCTG
    GTGGAGGACG CTTTAAACAA CCCCACGATA TTACACTGCT CAGCCAAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.