AGRN gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005217205.3	XP_005217262.1	agrin isoform X1
XM_002694193.4	XP_002694239.1	agrin isoform X2
XM_005194858.3	XP_005194915.1	agrin isoform X1
XM_005217205.4	XP_005217262.1	agrin isoform X1
XM_002694193.5	XP_002694239.1	agrin isoform X2
XM_604151.8	XP_604151.4	agrin isoform X2
XM_005217206.3	XP_005217263.1	agrin isoform X3
XM_024976824.1	XP_024832592.1	agrin isoform X4
XM_005194859.3	XP_005194916.1	agrin isoform X3
XM_005217206.4	XP_005217263.1	agrin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa223999	XM_024976824.1 Latest version!	Bos taurus agrin (AGRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa08756	XM_005194859.3 Latest version!	Bos taurus agrin (AGRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa08756	XM_005217206.4 Latest version!	Bos taurus agrin (AGRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa08754	XM_005194858.3 Latest version!	Bos taurus agrin (AGRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa08754	XM_005217205.4 Latest version!	Bos taurus agrin (AGRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa08755	XM_002694193.5 Latest version!	Bos taurus agrin (AGRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa08755	XM_604151.8 Latest version!	Bos taurus agrin (AGRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa08754	XM_005217205.3	Bos taurus agrin (AGRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa08755	XM_002694193.4	Bos taurus agrin (AGRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa08756	XM_005217206.3	Bos taurus agrin (AGRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

AGRN ( XM_024976824.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832592.1 Bos taurus agrin (AGRN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa223999
Clone ID Related Accession (Same CDS sequence)	XM_024976824.1
Accession Version	XM_024976824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC ACGGGGTCTC CGTCCTGGTG 
CGGTATTTCA TGATCCCGTG TAACGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC 
TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC 
CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TCGGCGCTGT CTGCGAGCCC 
AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCTCCTG CCCCAGCGTG 
GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGAG GCGCATCCGT TTGCTGCGCC GCGGGCCCTG CGGGACCCGG 
GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCTTC TGCGGATGGA 
CTGACAGCCA CGTGCCTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAAGG CCCTGTGTGC 
GGCAGCGACG GCAGTGACTA CCCCAGCGAG TGCCAGCTCT TACGCCACGC CTGTGCCCAC 
CAAGAGAACA TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTTCCTCAGT 
GACCTGAGCC GTACCTGCCG TGTGAACCCA CGCACGCGGC GGCCGGAGAT GCTCCTGCGA 
CCAGAGAGCT GCCCTCCCCG TGGGACACCT GTGTGTGGGG ACGACGGGGT TACTTACGAT 
AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCAAGGCA TCCTCCTGCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC 
CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGCGACCGCG TCGTCTGTGA CGGGGCCTAC 
AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC 
GAGTGTCAGC AGCAGCGTAG CATTCCCATG AAGCACCAGG GCCCGTGTGA CCAGTCCCCA 
TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCAATGT GTGCTGTGAA GAATGGGGAA 
GCCGAATGTG CGTGTCCGAA GGCGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT 
GGCATCACAT ATGGCAGTGC GTGTGAACTG GAAGCCACAG CCTGTGCCCT TGGGCGGGAG 
ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG 
TGTGAAGCTG AGACCGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGCT CTGATGGGCG CACGTATGCC AGTGAATGTG AACTACACGT CCACGCCTGC 
ACACACCAGA TCAGTCTGCA CGTGGCCTCG GCTGGTCCTT GCCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC 
CCTCCACCCG GCCCCGTGTG TGGCAGCGAT GGCGTGACCT ACGGCAGTTC ATGCGAGCTC 
CGTGAGGCTG CCTGTCAGCA GCAAACACAG ATCGAGGAGG CCTGGGCGGG GCCGTGTGAG 
CAAGCTGAGT GTGGCTCCGG AGGCTCGGGC TCGGGAGAGG ACGGCGAGTG TGAGCCTGAG 
CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGACGGGCC ATGCGTCTGC 
GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC 
CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG 
GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCGTTGC CACCTGCGAT TCCCCTGCAC 
TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTTGGC 
CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTACGG CGGCACCTGT 
GACCCTGTCA CAGGTCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ACGGCCGGAG CGGCTGTACC 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA 
TGTTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCC 
CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCCACCCA AGACCTGTGC TGAGATGCTT 
TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA 
CCCACCTGTC CAGGGGCCGA TGCTACCAAG GTCTGCGGGT CAGATGGAGT CACTTATGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAAAGC 
TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCCCGTG TGACATCCGC ATCTGTGGCT 
CCCTCAGCAC TTGACCTGAA CAAGGCACCA TTGCCGCCCC CCAGCGCCTT GCCCCTGGCT 
CCCAGCAGTA CCCGCCACAG CCAGCCCACT TCTCGAGCCT CATCACAACC TTGGACCATG 
GCCAGCATCC CCAGGACCAC TGCAAGGCCA GTGCTGACCA TGCCTCCCGT GGCCCCCTCC 
CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGTGGT 
GACGAGGAGC TGAGTGGTGA CTTGGAGGCC AGTGGGGCTG GCTCAGGAGG ACTTGAGTCC 
CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC CTGCTACAAC 
TCGCCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC 
TGTCCTGCCA CCAAGGTGTT CCAGGGTGTG CTGGAGCTTG AGGGGGTCGA GGGGCAAGAA 
CTGTTCTACA CGCCGGAGAT GGCAGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG 
AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAACTCGG ACGTTAAGAA GGACTTCCGA 
AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC 
TTTGATCCCA CCACAGCCTT CAGGGCGTCT GACGTGGGCC GGGCCCTGCT CCGGCAGCTC 
CAGGCGTCCA GGCGCAGGTC CCTGGGCGTG AGGCGGCCTC TGCAAGAACA TGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACGGCC 
AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC 
AGTCACACAA GCCGGCCCGT CAGCAGGACC ACACCTCCCG CGACCACGCG CCAGCCCCCC 
ACCACTGCCC CTAGTCGTGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC 
CGAAGGCCCT GTGATTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCG 
GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACGGGGG GTGCCGTCTG TGAGAAGGCC 
CTGCACCCCT CCGTGCCAGC CTTTGGGGGC CACTCCTTCC TGGCCTTTCC CACCCTCCGT 
GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG 
CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGTGCTGCT GGGAGGCCGT 
GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACCAGCTC CGTGCCCGTG 
CAGCCCGGCC GCTGGCATCA CCTGGAGCTG TCTCGGCACT GGCGCCAAGG CACTCTCTCC 
GTGGACGGTG AGACTCCTGT TCTGGGCCAG AGCCCCAGTG GCACTGACGG TCTCAACCTG 
GACACTGATC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG 
ACCTCTGTCA GCATCGGTCT TAGGGGTTGT ATCCGCTTGC TGGATGTCAA CAATCAGCGG 
CTGGAGCTGA GCAGCTGGCC AGAGTCTGCG ACTCGAAGCT CCGGAGTGGG CAAGTGCGGG 
GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA 
GGCAGGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGCC CAACCTGTGC CGACGAGAAG 
GACCCTTGTC AGCCGAACCC CTGCCATGGA GCAGCCCCCT GCCGCGTGCT GCCCCAGGGA 
GAGGCCAAGT GCGAGTGCCC CCATGGCCGG GAGGGCAGCC TCTGCCAGAC AGTCTCAGAG 
CCGGAGGACA ACCAGCCGTT CCTGGCTGAT TTCAGCAGCT TCTCCTACCT GGAACTGAAA 
GGCCTGCACA CCTTTGAGCG GGACCTGGGG GAGAAGATGG CACTGGAGGT GGTGTTCCTG 
GCTCGGAGCC CCAGCGGCCT GCTCCTCTAT AATGGACAGA AGACAGATGG CAAGGGCGAC 
TTTGTGTCCC TGGCGCTGCA CAATGGCCTC CTGGAGTTCC GCTACGATCT GGGCAAGGGG 
GCAGCGGTCA TCAGGAGCAA GGAGCCAGTG GCCCTGGGTG CCTGGACCAG GGTCTCCCTG 
GAGCGAAATG GACGCAAAGG AGCCATGCGG GTTGGTGATG GGCCTCGCGT GCTGGGGGAG 
TCCCCGAAAT CCCGCAAGGT GCCACACACC GTTCTCAACC TAAAGGAGCC GCTCTTTGTT 
GGGGGGGCCC CTGACTTCAG CAAGCTGGCC CGAGCAGCTG CTGTGTCCTC TGGCTTTGAT 
GGAGCCATCC AGCTGGTCTC CCTGAATGGC CGCCAACTGC TGACCCGTGA GAACGTGGTC 
CGGGCCGTGG ACGTCTCGTC CTTTGCAGAC CACCCTTGTA CCCAGGCTGA GGGTCAGCCT 
TGCCTCCATG GGGCTTCCTG TCTCCCCCGC GAGGCCTCCT ATGAGTGCCT GTGTCCTGCA 
GGCTTCTCAG GGCTGCACTG TGAGAAGGGC TTGATTGAGA AGTCAGCTGG GGACCTGGAC 
GCACTGGCCT TTGATGGACG GACCTACATC GAGTACCTCA ACGCTGTGAC CGAGAGTGAG 
AAGGCACTCC AGAGCAACCA CTTTGAGCTG AGCCTGCGCA CTGAGGCCAC ACAGGGGCTG 
GTGCTGTGGA GTGGCAAGGC CACGGAGCGG GCTGACTACA TCGCACTGGC CATCGTGGAC 
GGGCGCCTGC AACTGGCTTA TGACCTGGGC TCCCAGCCCG TGGTGTTGCG CTCCACCGTA 
CCAGTGAATA CCAACCGCTG GTTGCGGGTC AGGGCACACA GGAAACAGAG GGAAGGTTCC 
CTTCAGGTGG GCAACGAGGC CCCTGTGACT GGCTCCTCCC CACTGGGTGC CACGCAGCTG 
GACACAGATG GAGCCCTGTG GCTGGGTGGC CTGGAGAAGC TGCCCACAGG CCAGGCCCTG 
CCCAAAGCCT ATGGCACAGG CTTCGTGGGC TGCCTGCGGG ACGTGGTCGT GGGCCAGCGC 
CCGGTGCACC TGCTGGAGGA CGCCATCACC AAGCCAGAGC TTCGGCCCTG CCCTGCCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832592.1
CDS	1..5823
Translation	MPPLLLERDRRQRHGVSVLVRYFMIPCNVCLILLATATLGFAVLLFLNNYKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_024976824.1
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976824.1

ORF Insert Sequence:

ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC ACGGGGTCTC CGTCCTGGTG 
CGGTATTTCA TGATCCCGTG TAACGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC 
TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC 
CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TCGGCGCTGT CTGCGAGCCC 
AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCTCCTG CCCCAGCGTG 
GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGAG GCGCATCCGT TTGCTGCGCC GCGGGCCCTG CGGGACCCGG 
GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCTTC TGCGGATGGA 
CTGACAGCCA CGTGCCTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAAGG CCCTGTGTGC 
GGCAGCGACG GCAGTGACTA CCCCAGCGAG TGCCAGCTCT TACGCCACGC CTGTGCCCAC 
CAAGAGAACA TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTTCCTCAGT 
GACCTGAGCC GTACCTGCCG TGTGAACCCA CGCACGCGGC GGCCGGAGAT GCTCCTGCGA 
CCAGAGAGCT GCCCTCCCCG TGGGACACCT GTGTGTGGGG ACGACGGGGT TACTTACGAT 
AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCAAGGCA TCCTCCTGCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC 
CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGCGACCGCG TCGTCTGTGA CGGGGCCTAC 
AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC 
GAGTGTCAGC AGCAGCGTAG CATTCCCATG AAGCACCAGG GCCCGTGTGA CCAGTCCCCA 
TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCAATGT GTGCTGTGAA GAATGGGGAA 
GCCGAATGTG CGTGTCCGAA GGCGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT 
GGCATCACAT ATGGCAGTGC GTGTGAACTG GAAGCCACAG CCTGTGCCCT TGGGCGGGAG 
ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG 
TGTGAAGCTG AGACCGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGCT CTGATGGGCG CACGTATGCC AGTGAATGTG AACTACACGT CCACGCCTGC 
ACACACCAGA TCAGTCTGCA CGTGGCCTCG GCTGGTCCTT GCCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC 
CCTCCACCCG GCCCCGTGTG TGGCAGCGAT GGCGTGACCT ACGGCAGTTC ATGCGAGCTC 
CGTGAGGCTG CCTGTCAGCA GCAAACACAG ATCGAGGAGG CCTGGGCGGG GCCGTGTGAG 
CAAGCTGAGT GTGGCTCCGG AGGCTCGGGC TCGGGAGAGG ACGGCGAGTG TGAGCCTGAG 
CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGACGGGCC ATGCGTCTGC 
GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC 
CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG 
GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCGTTGC CACCTGCGAT TCCCCTGCAC 
TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTTGGC 
CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTACGG CGGCACCTGT 
GACCCTGTCA CAGGTCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ACGGCCGGAG CGGCTGTACC 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA 
TGTTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCC 
CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCCACCCA AGACCTGTGC TGAGATGCTT 
TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA 
CCCACCTGTC CAGGGGCCGA TGCTACCAAG GTCTGCGGGT CAGATGGAGT CACTTATGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAAAGC 
TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCCCGTG TGACATCCGC ATCTGTGGCT 
CCCTCAGCAC TTGACCTGAA CAAGGCACCA TTGCCGCCCC CCAGCGCCTT GCCCCTGGCT 
CCCAGCAGTA CCCGCCACAG CCAGCCCACT TCTCGAGCCT CATCACAACC TTGGACCATG 
GCCAGCATCC CCAGGACCAC TGCAAGGCCA GTGCTGACCA TGCCTCCCGT GGCCCCCTCC 
CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGTGGT 
GACGAGGAGC TGAGTGGTGA CTTGGAGGCC AGTGGGGCTG GCTCAGGAGG ACTTGAGTCC 
CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC CTGCTACAAC 
TCGCCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC 
TGTCCTGCCA CCAAGGTGTT CCAGGGTGTG CTGGAGCTTG AGGGGGTCGA GGGGCAAGAA 
CTGTTCTACA CGCCGGAGAT GGCAGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG 
AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAACTCGG ACGTTAAGAA GGACTTCCGA 
AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC 
TTTGATCCCA CCACAGCCTT CAGGGCGTCT GACGTGGGCC GGGCCCTGCT CCGGCAGCTC 
CAGGCGTCCA GGCGCAGGTC CCTGGGCGTG AGGCGGCCTC TGCAAGAACA TGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACGGCC 
AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC 
AGTCACACAA GCCGGCCCGT CAGCAGGACC ACACCTCCCG CGACCACGCG CCAGCCCCCC 
ACCACTGCCC CTAGTCGTGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC 
CGAAGGCCCT GTGATTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCG 
GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACGGGGG GTGCCGTCTG TGAGAAGGCC 
CTGCACCCCT CCGTGCCAGC CTTTGGGGGC CACTCCTTCC TGGCCTTTCC CACCCTCCGT 
GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG 
CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGTGCTGCT GGGAGGCCGT 
GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACCAGCTC CGTGCCCGTG 
CAGCCCGGCC GCTGGCATCA CCTGGAGCTG TCTCGGCACT GGCGCCAAGG CACTCTCTCC 
GTGGACGGTG AGACTCCTGT TCTGGGCCAG AGCCCCAGTG GCACTGACGG TCTCAACCTG 
GACACTGATC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG 
ACCTCTGTCA GCATCGGTCT TAGGGGTTGT ATCCGCTTGC TGGATGTCAA CAATCAGCGG 
CTGGAGCTGA GCAGCTGGCC AGAGTCTGCG ACTCGAAGCT CCGGAGTGGG CAAGTGCGGG 
GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA 
GGCAGGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGCC CAACCTGTGC CGACGAGAAG 
GACCCTTGTC AGCCGAACCC CTGCCATGGA GCAGCCCCCT GCCGCGTGCT GCCCCAGGGA 
GAGGCCAAGT GCGAGTGCCC CCATGGCCGG GAGGGCAGCC TCTGCCAGAC AGTCTCAGAG 
CCGGAGGACA ACCAGCCGTT CCTGGCTGAT TTCAGCAGCT TCTCCTACCT GGAACTGAAA 
GGCCTGCACA CCTTTGAGCG GGACCTGGGG GAGAAGATGG CACTGGAGGT GGTGTTCCTG 
GCTCGGAGCC CCAGCGGCCT GCTCCTCTAT AATGGACAGA AGACAGATGG CAAGGGCGAC 
TTTGTGTCCC TGGCGCTGCA CAATGGCCTC CTGGAGTTCC GCTACGATCT GGGCAAGGGG 
GCAGCGGTCA TCAGGAGCAA GGAGCCAGTG GCCCTGGGTG CCTGGACCAG GGTCTCCCTG 
GAGCGAAATG GACGCAAAGG AGCCATGCGG GTTGGTGATG GGCCTCGCGT GCTGGGGGAG 
TCCCCGAAAT CCCGCAAGGT GCCACACACC GTTCTCAACC TAAAGGAGCC GCTCTTTGTT 
GGGGGGGCCC CTGACTTCAG CAAGCTGGCC CGAGCAGCTG CTGTGTCCTC TGGCTTTGAT 
GGAGCCATCC AGCTGGTCTC CCTGAATGGC CGCCAACTGC TGACCCGTGA GAACGTGGTC 
CGGGCCGTGG ACGTCTCGTC CTTTGCAGAC CACCCTTGTA CCCAGGCTGA GGGTCAGCCT 
TGCCTCCATG GGGCTTCCTG TCTCCCCCGC GAGGCCTCCT ATGAGTGCCT GTGTCCTGCA 
GGCTTCTCAG GGCTGCACTG TGAGAAGGGC TTGATTGAGA AGTCAGCTGG GGACCTGGAC 
GCACTGGCCT TTGATGGACG GACCTACATC GAGTACCTCA ACGCTGTGAC CGAGAGTGAG 
AAGGCACTCC AGAGCAACCA CTTTGAGCTG AGCCTGCGCA CTGAGGCCAC ACAGGGGCTG 
GTGCTGTGGA GTGGCAAGGC CACGGAGCGG GCTGACTACA TCGCACTGGC CATCGTGGAC 
GGGCGCCTGC AACTGGCTTA TGACCTGGGC TCCCAGCCCG TGGTGTTGCG CTCCACCGTA 
CCAGTGAATA CCAACCGCTG GTTGCGGGTC AGGGCACACA GGAAACAGAG GGAAGGTTCC 
CTTCAGGTGG GCAACGAGGC CCCTGTGACT GGCTCCTCCC CACTGGGTGC CACGCAGCTG 
GACACAGATG GAGCCCTGTG GCTGGGTGGC CTGGAGAAGC TGCCCACAGG CCAGGCCCTG 
CCCAAAGCCT ATGGCACAGG CTTCGTGGGC TGCCTGCGGG ACGTGGTCGT GGGCCAGCGC 
CCGGTGCACC TGCTGGAGGA CGCCATCACC AAGCCAGAGC TTCGGCCCTG CCCTGCCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005217206.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217263.1 Bos taurus agrin (AGRN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa08756
Clone ID Related Accession (Same CDS sequence)	XM_005217206.3 , XM_005194859.3 , XM_005217206.4
Accession Version	XM_005217206.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217206.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC ACGGGGTCTC CGTCCTGGTG 
CGGTATTTCA TGATCCCGTG TAACGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC 
TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC 
CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TCGGCGCTGT CTGCGAGCCC 
AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCTCCTG CCCCAGCGTG 
GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGAG GCGCATCCGT TTGCTGCGCC GCGGGCCCTG CGGGACCCGG 
GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCTTC TGCGGATGGA 
CTGACAGCCA CGTGCCTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAAGG CCCTGTGTGC 
GGCAGCGACG GCAGTGACTA CCCCAGCGAG TGCCAGCTCT TACGCCACGC CTGTGCCCAC 
CAAGAGAACA TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTTCCTCAGT 
GACCTGAGCC GTACCTGCCG TGTGAACCCA CGCACGCGGC GGCCGGAGAT GCTCCTGCGA 
CCAGAGAGCT GCCCTCCCCG TGGGACACCT GTGTGTGGGG ACGACGGGGT TACTTACGAT 
AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCAAGGCA TCCTCCTGCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC 
CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGCGACCGCG TCGTCTGTGA CGGGGCCTAC 
AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC 
GAGTGTCAGC AGCAGCGTAG CATTCCCATG AAGCACCAGG GCCCGTGTGA CCAGTCCCCA 
TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCAATGT GTGCTGTGAA GAATGGGGAA 
GCCGAATGTG CGTGTCCGAA GGCGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT 
GGCATCACAT ATGGCAGTGC GTGTGAACTG GAAGCCACAG CCTGTGCCCT TGGGCGGGAG 
ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG 
TGTGAAGCTG AGACCGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGCT CTGATGGGCG CACGTATGCC AGTGAATGTG AACTACACGT CCACGCCTGC 
ACACACCAGA TCAGTCTGCA CGTGGCCTCG GCTGGTCCTT GCCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC 
CCTCCACCCG GCCCCGTGTG TGGCAGCGAT GGCGTGACCT ACGGCAGTTC ATGCGAGCTC 
CGTGAGGCTG CCTGTCAGCA GCAAACACAG ATCGAGGAGG CCTGGGCGGG GCCGTGTGAG 
CAAGCTGAGT GTGGCTCCGG AGGCTCGGGC TCGGGAGAGG ACGGCGAGTG TGAGCCTGAG 
CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGACGGGCC ATGCGTCTGC 
GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC 
CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG 
GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCGTTGC CACCTGCGAT TCCCCTGCAC 
TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTTGGC 
CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTACGG CGGCACCTGT 
GACCCTGTCA CAGGTCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ACGGCCGGAG CGGCTGTACC 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA 
TGTTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCC 
CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCCACCCA AGACCTGTGC TGAGATGCTT 
TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA 
CCCACCTGTC CAGGGGCCGA TGCTACCAAG GTCTGCGGGT CAGATGGAGT CACTTATGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAAAGC 
TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCCCGTG TGACATCCGC ATCTGTGGCT 
CCCTCAGCAC TTGACCTGAA CAAGGCACCA TTGCCGCCCC CCAGCGCCTT GCCCCTGGCT 
CCCAGCAGTA CCCGCCACAG CCAGCCCACT TCTCGAGCCT CATCACAACC TTGGACCATG 
GCCAGCATCC CCAGGACCAC TGCAAGGCCA GTGCTGACCA TGCCTCCCGT GGCCCCCTCC 
CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGTGGT 
GACGAGGAGC TGAGTGGTGA CTTGGAGGCC AGTGGGGCTG GCTCAGGAGG ACTTGAGTCC 
CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC CTGCTACAAC 
TCGCCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC 
TGTCCTGCCA CCAAGGTGTT CCAGGGTGTG CTGGAGCTTG AGGGGGTCGA GGGGCAAGAA 
CTGTTCTACA CGCCGGAGAT GGCAGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG 
AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAACTCGG ACGTTAAGAA GGACTTCCGA 
AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC 
TTTGATCCCA CCACAGCCTT CAGGGCGTCT GACGTGGGCC GGGCCCTGCT CCGGCAGCTC 
CAGGCGTCCA GGCGCAGGTC CCTGGGCGTG AGGCGGCCTC TGCAAGAACA TGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACGGCC 
AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC 
AGTCACACAA GCCGGCCCGT CAGCAGGACC ACACCTCCCG CGACCACGCG CCAGCCCCCC 
ACCACTGCCC CTAGTCGTGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC 
CGAAGGCCCT GTGATTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCG 
GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACGGGGG GTGCCGTCTG TGAGAAGGCC 
CTGCACCCCT CCGTGCCAGC CTTTGGGGGC CACTCCTTCC TGGCCTTTCC CACCCTCCGT 
GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG 
CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGTGCTGCT GGGAGGCCGT 
GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACCAGCTC CGTGCCCGTG 
CAGCCCGGCC GCTGGCATCA CCTGGAGCTG TCTCGGCACT GGCGCCAAGG CACTCTCTCC 
GTGGACGGTG AGACTCCTGT TCTGGGCCAG AGCCCCAGTG GCACTGACGG TCTCAACCTG 
GACACTGATC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG 
ACCTCTGTCA GCATCGGTCT TAGGGGTTGT ATCCGCTTGC TGGATGTCAA CAATCAGCGG 
CTGGAGCTGA GCAGCTGGCC AGAGTCTGCG ACTCGAAGCT CCGGAGTGGG CAAGTGCGGG 
GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA 
GGCAGGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGCC CAACCTGTGC CGACGAGAAG 
GACCCTTGTC AGCCGAACCC CTGCCATGGA GCAGCCCCCT GCCGCGTGCT GCCCCAGGGA 
GAGGCCAAGT GCGAGTGCCC CCATGGCCGG GAGGGCAGCC TCTGCCAGAC AGTCTCAGAG 
CCGGAGGACA ACCAGCCGTT CCTGGCTGAT TTCAGCAGCT TCTCCTACCT GGAACTGAAA 
GGCCTGCACA CCTTTGAGCG GGACCTGGGG GAGAAGATGG CACTGGAGGT GGTGTTCCTG 
GCTCGGAGCC CCAGCGGCCT GCTCCTCTAT AATGGACAGA AGACAGATGG CAAGGGCGAC 
TTTGTGTCCC TGGCGCTGCA CAATGGCCTC CTGGAGTTCC GCTACGATCT GGGCAAGGGG 
GCAGCGGTCA TCAGGAGCAA GGAGCCAGTG GCCCTGGGTG CCTGGACCAG GGTCTCCCTG 
GAGCGAAATG GACGCAAAGG AGCCATGCGG GTTGGTGATG GGCCTCGCGT GCTGGGGGAG 
TCCCCGAAAT CCCGCAAGGT GCCACACACC GTTCTCAACC TAAAGGAGCC GCTCTTTGTT 
GGGGGGGCCC CTGACTTCAG CAAGCTGGCC CGAGCAGCTG CTGTGTCCTC TGGCTTTGAT 
GGAGCCATCC AGCTGGTCTC CCTGAATGGC CGCCAACTGC TGACCCGTGA GAACGTGGTC 
CGGGCCGTGG ACGTCTCGTC CTTTGCAGAC CACCCTTGTA CCCAGGCTGA GGGTCAGCCT 
TGCCTCCATG GGGCTTCCTG TCTCCCCCGC GAGGCCTCCT ATGAGTGCCT GTGTCCTGCA 
GGCTTCTCAG GGCTGCACTG TGAGAAGGGC TTGATTGAGA AGTCAGCTGG GGACCTGGAC 
GCACTGGCCT TTGATGGACG GACCTACATC GAGTACCTCA ACGCTGTGAC CGAGAGCGAA 
CTGACCAATG AGATCCCAGC CCCTGAAACT CTGGATTCCG GGGCCCATCC CAGTGAGAAG 
GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG 
CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG 
CGCCTGCAAC TGGCTTATGA CCTGGGCTCC CAGCCCGTGG TGTTGCGCTC CACCGTACCA 
GTGAATACCA ACCGCTGGTT GCGGGTCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT 
CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC 
ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GAGAAGCTGC CCACAGGCCA GGCCCTGCCC 
AAAGCCTATG GCACAGGCTT CGTGGGCTGC CTGCGGGACG TGGTCGTGGG CCAGCGCCCG 
GTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217263.1
CDS	26..5905
Translation	MPPLLLERDRRQRHGVSVLVRYFMIPCNVCLILLATATLGFAVLLFLNNYKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESELTNEIPAPETLDSGAHPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_005217206.3
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217206.3

ORF Insert Sequence:

ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC ACGGGGTCTC CGTCCTGGTG 
CGGTATTTCA TGATCCCGTG TAACGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC 
TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC 
CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TCGGCGCTGT CTGCGAGCCC 
AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCTCCTG CCCCAGCGTG 
GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGAG GCGCATCCGT TTGCTGCGCC GCGGGCCCTG CGGGACCCGG 
GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCTTC TGCGGATGGA 
CTGACAGCCA CGTGCCTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAAGG CCCTGTGTGC 
GGCAGCGACG GCAGTGACTA CCCCAGCGAG TGCCAGCTCT TACGCCACGC CTGTGCCCAC 
CAAGAGAACA TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTTCCTCAGT 
GACCTGAGCC GTACCTGCCG TGTGAACCCA CGCACGCGGC GGCCGGAGAT GCTCCTGCGA 
CCAGAGAGCT GCCCTCCCCG TGGGACACCT GTGTGTGGGG ACGACGGGGT TACTTACGAT 
AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCAAGGCA TCCTCCTGCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC 
CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGCGACCGCG TCGTCTGTGA CGGGGCCTAC 
AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC 
GAGTGTCAGC AGCAGCGTAG CATTCCCATG AAGCACCAGG GCCCGTGTGA CCAGTCCCCA 
TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCAATGT GTGCTGTGAA GAATGGGGAA 
GCCGAATGTG CGTGTCCGAA GGCGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT 
GGCATCACAT ATGGCAGTGC GTGTGAACTG GAAGCCACAG CCTGTGCCCT TGGGCGGGAG 
ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG 
TGTGAAGCTG AGACCGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGCT CTGATGGGCG CACGTATGCC AGTGAATGTG AACTACACGT CCACGCCTGC 
ACACACCAGA TCAGTCTGCA CGTGGCCTCG GCTGGTCCTT GCCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC 
CCTCCACCCG GCCCCGTGTG TGGCAGCGAT GGCGTGACCT ACGGCAGTTC ATGCGAGCTC 
CGTGAGGCTG CCTGTCAGCA GCAAACACAG ATCGAGGAGG CCTGGGCGGG GCCGTGTGAG 
CAAGCTGAGT GTGGCTCCGG AGGCTCGGGC TCGGGAGAGG ACGGCGAGTG TGAGCCTGAG 
CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGACGGGCC ATGCGTCTGC 
GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC 
CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG 
GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCGTTGC CACCTGCGAT TCCCCTGCAC 
TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTTGGC 
CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTACGG CGGCACCTGT 
GACCCTGTCA CAGGTCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ACGGCCGGAG CGGCTGTACC 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA 
TGTTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCC 
CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCCACCCA AGACCTGTGC TGAGATGCTT 
TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA 
CCCACCTGTC CAGGGGCCGA TGCTACCAAG GTCTGCGGGT CAGATGGAGT CACTTATGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAAAGC 
TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCCCGTG TGACATCCGC ATCTGTGGCT 
CCCTCAGCAC TTGACCTGAA CAAGGCACCA TTGCCGCCCC CCAGCGCCTT GCCCCTGGCT 
CCCAGCAGTA CCCGCCACAG CCAGCCCACT TCTCGAGCCT CATCACAACC TTGGACCATG 
GCCAGCATCC CCAGGACCAC TGCAAGGCCA GTGCTGACCA TGCCTCCCGT GGCCCCCTCC 
CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGTGGT 
GACGAGGAGC TGAGTGGTGA CTTGGAGGCC AGTGGGGCTG GCTCAGGAGG ACTTGAGTCC 
CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC CTGCTACAAC 
TCGCCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC 
TGTCCTGCCA CCAAGGTGTT CCAGGGTGTG CTGGAGCTTG AGGGGGTCGA GGGGCAAGAA 
CTGTTCTACA CGCCGGAGAT GGCAGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG 
AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAACTCGG ACGTTAAGAA GGACTTCCGA 
AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC 
TTTGATCCCA CCACAGCCTT CAGGGCGTCT GACGTGGGCC GGGCCCTGCT CCGGCAGCTC 
CAGGCGTCCA GGCGCAGGTC CCTGGGCGTG AGGCGGCCTC TGCAAGAACA TGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACGGCC 
AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC 
AGTCACACAA GCCGGCCCGT CAGCAGGACC ACACCTCCCG CGACCACGCG CCAGCCCCCC 
ACCACTGCCC CTAGTCGTGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC 
CGAAGGCCCT GTGATTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCG 
GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACGGGGG GTGCCGTCTG TGAGAAGGCC 
CTGCACCCCT CCGTGCCAGC CTTTGGGGGC CACTCCTTCC TGGCCTTTCC CACCCTCCGT 
GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG 
CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGTGCTGCT GGGAGGCCGT 
GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACCAGCTC CGTGCCCGTG 
CAGCCCGGCC GCTGGCATCA CCTGGAGCTG TCTCGGCACT GGCGCCAAGG CACTCTCTCC 
GTGGACGGTG AGACTCCTGT TCTGGGCCAG AGCCCCAGTG GCACTGACGG TCTCAACCTG 
GACACTGATC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG 
ACCTCTGTCA GCATCGGTCT TAGGGGTTGT ATCCGCTTGC TGGATGTCAA CAATCAGCGG 
CTGGAGCTGA GCAGCTGGCC AGAGTCTGCG ACTCGAAGCT CCGGAGTGGG CAAGTGCGGG 
GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA 
GGCAGGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGCC CAACCTGTGC CGACGAGAAG 
GACCCTTGTC AGCCGAACCC CTGCCATGGA GCAGCCCCCT GCCGCGTGCT GCCCCAGGGA 
GAGGCCAAGT GCGAGTGCCC CCATGGCCGG GAGGGCAGCC TCTGCCAGAC AGTCTCAGAG 
CCGGAGGACA ACCAGCCGTT CCTGGCTGAT TTCAGCAGCT TCTCCTACCT GGAACTGAAA 
GGCCTGCACA CCTTTGAGCG GGACCTGGGG GAGAAGATGG CACTGGAGGT GGTGTTCCTG 
GCTCGGAGCC CCAGCGGCCT GCTCCTCTAT AATGGACAGA AGACAGATGG CAAGGGCGAC 
TTTGTGTCCC TGGCGCTGCA CAATGGCCTC CTGGAGTTCC GCTACGATCT GGGCAAGGGG 
GCAGCGGTCA TCAGGAGCAA GGAGCCAGTG GCCCTGGGTG CCTGGACCAG GGTCTCCCTG 
GAGCGAAATG GACGCAAAGG AGCCATGCGG GTTGGTGATG GGCCTCGCGT GCTGGGGGAG 
TCCCCGAAAT CCCGCAAGGT GCCACACACC GTTCTCAACC TAAAGGAGCC GCTCTTTGTT 
GGGGGGGCCC CTGACTTCAG CAAGCTGGCC CGAGCAGCTG CTGTGTCCTC TGGCTTTGAT 
GGAGCCATCC AGCTGGTCTC CCTGAATGGC CGCCAACTGC TGACCCGTGA GAACGTGGTC 
CGGGCCGTGG ACGTCTCGTC CTTTGCAGAC CACCCTTGTA CCCAGGCTGA GGGTCAGCCT 
TGCCTCCATG GGGCTTCCTG TCTCCCCCGC GAGGCCTCCT ATGAGTGCCT GTGTCCTGCA 
GGCTTCTCAG GGCTGCACTG TGAGAAGGGC TTGATTGAGA AGTCAGCTGG GGACCTGGAC 
GCACTGGCCT TTGATGGACG GACCTACATC GAGTACCTCA ACGCTGTGAC CGAGAGCGAA 
CTGACCAATG AGATCCCAGC CCCTGAAACT CTGGATTCCG GGGCCCATCC CAGTGAGAAG 
GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG 
CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG 
CGCCTGCAAC TGGCTTATGA CCTGGGCTCC CAGCCCGTGG TGTTGCGCTC CACCGTACCA 
GTGAATACCA ACCGCTGGTT GCGGGTCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT 
CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC 
ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GAGAAGCTGC CCACAGGCCA GGCCCTGCCC 
AAAGCCTATG GCACAGGCTT CGTGGGCTGC CTGCGGGACG TGGTCGTGGG CCAGCGCCCG 
GTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005194859.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194916.1 Bos taurus agrin (AGRN), transcript variant X3, mRNA.

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CloneID	OBa08756
Clone ID Related Accession (Same CDS sequence)	XM_005217206.3 , XM_005194859.3 , XM_005217206.4
Accession Version	XM_005194859.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194859.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC ACGGGGTCTC CGTCCTGGTG 
CGGTATTTCA TGATCCCGTG TAACGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC 
TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC 
CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TCGGCGCTGT CTGCGAGCCC 
AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCTCCTG CCCCAGCGTG 
GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGAG GCGCATCCGT TTGCTGCGCC GCGGGCCCTG CGGGACCCGG 
GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCTTC TGCGGATGGA 
CTGACAGCCA CGTGCCTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAAGG CCCTGTGTGC 
GGCAGCGACG GCAGTGACTA CCCCAGCGAG TGCCAGCTCT TACGCCACGC CTGTGCCCAC 
CAAGAGAACA TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTTCCTCAGT 
GACCTGAGCC GTACCTGCCG TGTGAACCCA CGCACGCGGC GGCCGGAGAT GCTCCTGCGA 
CCAGAGAGCT GCCCTCCCCG TGGGACACCT GTGTGTGGGG ACGACGGGGT TACTTACGAT 
AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCAAGGCA TCCTCCTGCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC 
CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGCGACCGCG TCGTCTGTGA CGGGGCCTAC 
AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC 
GAGTGTCAGC AGCAGCGTAG CATTCCCATG AAGCACCAGG GCCCGTGTGA CCAGTCCCCA 
TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCAATGT GTGCTGTGAA GAATGGGGAA 
GCCGAATGTG CGTGTCCGAA GGCGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT 
GGCATCACAT ATGGCAGTGC GTGTGAACTG GAAGCCACAG CCTGTGCCCT TGGGCGGGAG 
ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG 
TGTGAAGCTG AGACCGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGCT CTGATGGGCG CACGTATGCC AGTGAATGTG AACTACACGT CCACGCCTGC 
ACACACCAGA TCAGTCTGCA CGTGGCCTCG GCTGGTCCTT GCCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC 
CCTCCACCCG GCCCCGTGTG TGGCAGCGAT GGCGTGACCT ACGGCAGTTC ATGCGAGCTC 
CGTGAGGCTG CCTGTCAGCA GCAAACACAG ATCGAGGAGG CCTGGGCGGG GCCGTGTGAG 
CAAGCTGAGT GTGGCTCCGG AGGCTCGGGC TCGGGAGAGG ACGGCGAGTG TGAGCCTGAG 
CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGACGGGCC ATGCGTCTGC 
GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC 
CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG 
GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCGTTGC CACCTGCGAT TCCCCTGCAC 
TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTTGGC 
CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTACGG CGGCACCTGT 
GACCCTGTCA CAGGTCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ACGGCCGGAG CGGCTGTACC 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA 
TGTTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCC 
CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCCACCCA AGACCTGTGC TGAGATGCTT 
TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA 
CCCACCTGTC CAGGGGCCGA TGCTACCAAG GTCTGCGGGT CAGATGGAGT CACTTATGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAAAGC 
TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCCCGTG TGACATCCGC ATCTGTGGCT 
CCCTCAGCAC TTGACCTGAA CAAGGCACCA TTGCCGCCCC CCAGCGCCTT GCCCCTGGCT 
CCCAGCAGTA CCCGCCACAG CCAGCCCACT TCTCGAGCCT CATCACAACC TTGGACCATG 
GCCAGCATCC CCAGGACCAC TGCAAGGCCA GTGCTGACCA TGCCTCCCGT GGCCCCCTCC 
CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGTGGT 
GACGAGGAGC TGAGTGGTGA CTTGGAGGCC AGTGGGGCTG GCTCAGGAGG ACTTGAGTCC 
CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC CTGCTACAAC 
TCGCCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC 
TGTCCTGCCA CCAAGGTGTT CCAGGGTGTG CTGGAGCTTG AGGGGGTCGA GGGGCAAGAA 
CTGTTCTACA CGCCGGAGAT GGCAGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG 
AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAACTCGG ACGTTAAGAA GGACTTCCGA 
AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC 
TTTGATCCCA CCACAGCCTT CAGGGCGTCT GACGTGGGCC GGGCCCTGCT CCGGCAGCTC 
CAGGCGTCCA GGCGCAGGTC CCTGGGCGTG AGGCGGCCTC TGCAAGAACA TGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACGGCC 
AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC 
AGTCACACAA GCCGGCCCGT CAGCAGGACC ACACCTCCCG CGACCACGCG CCAGCCCCCC 
ACCACTGCCC CTAGTCGTGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC 
CGAAGGCCCT GTGATTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCG 
GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACGGGGG GTGCCGTCTG TGAGAAGGCC 
CTGCACCCCT CCGTGCCAGC CTTTGGGGGC CACTCCTTCC TGGCCTTTCC CACCCTCCGT 
GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG 
CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGTGCTGCT GGGAGGCCGT 
GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACCAGCTC CGTGCCCGTG 
CAGCCCGGCC GCTGGCATCA CCTGGAGCTG TCTCGGCACT GGCGCCAAGG CACTCTCTCC 
GTGGACGGTG AGACTCCTGT TCTGGGCCAG AGCCCCAGTG GCACTGACGG TCTCAACCTG 
GACACTGATC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG 
ACCTCTGTCA GCATCGGTCT TAGGGGTTGT ATCCGCTTGC TGGATGTCAA CAATCAGCGG 
CTGGAGCTGA GCAGCTGGCC AGAGTCTGCG ACTCGAAGCT CCGGAGTGGG CAAGTGCGGG 
GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA 
GGCAGGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGCC CAACCTGTGC CGACGAGAAG 
GACCCTTGTC AGCCGAACCC CTGCCATGGA GCAGCCCCCT GCCGCGTGCT GCCCCAGGGA 
GAGGCCAAGT GCGAGTGCCC CCATGGCCGG GAGGGCAGCC TCTGCCAGAC AGTCTCAGAG 
CCGGAGGACA ACCAGCCGTT CCTGGCTGAT TTCAGCAGCT TCTCCTACCT GGAACTGAAA 
GGCCTGCACA CCTTTGAGCG GGACCTGGGG GAGAAGATGG CACTGGAGGT GGTGTTCCTG 
GCTCGGAGCC CCAGCGGCCT GCTCCTCTAT AATGGACAGA AGACAGATGG CAAGGGCGAC 
TTTGTGTCCC TGGCGCTGCA CAATGGCCTC CTGGAGTTCC GCTACGATCT GGGCAAGGGG 
GCAGCGGTCA TCAGGAGCAA GGAGCCAGTG GCCCTGGGTG CCTGGACCAG GGTCTCCCTG 
GAGCGAAATG GACGCAAAGG AGCCATGCGG GTTGGTGATG GGCCTCGCGT GCTGGGGGAG 
TCCCCGAAAT CCCGCAAGGT GCCACACACC GTTCTCAACC TAAAGGAGCC GCTCTTTGTT 
GGGGGGGCCC CTGACTTCAG CAAGCTGGCC CGAGCAGCTG CTGTGTCCTC TGGCTTTGAT 
GGAGCCATCC AGCTGGTCTC CCTGAATGGC CGCCAACTGC TGACCCGTGA GAACGTGGTC 
CGGGCCGTGG ACGTCTCGTC CTTTGCAGAC CACCCTTGTA CCCAGGCTGA GGGTCAGCCT 
TGCCTCCATG GGGCTTCCTG TCTCCCCCGC GAGGCCTCCT ATGAGTGCCT GTGTCCTGCA 
GGCTTCTCAG GGCTGCACTG TGAGAAGGGC TTGATTGAGA AGTCAGCTGG GGACCTGGAC 
GCACTGGCCT TTGATGGACG GACCTACATC GAGTACCTCA ACGCTGTGAC CGAGAGCGAA 
CTGACCAATG AGATCCCAGC CCCTGAAACT CTGGATTCCG GGGCCCATCC CAGTGAGAAG 
GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG 
CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG 
CGCCTGCAAC TGGCTTATGA CCTGGGCTCC CAGCCCGTGG TGTTGCGCTC CACCGTACCA 
GTGAATACCA ACCGCTGGTT GCGGGTCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT 
CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC 
ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GAGAAGCTGC CCACAGGCCA GGCCCTGCCC 
AAAGCCTATG GCACAGGCTT CGTGGGCTGC CTGCGGGACG TGGTCGTGGG CCAGCGCCCG 
GTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194916.1
CDS	26..5905
Translation	MPPLLLERDRRQRHGVSVLVRYFMIPCNVCLILLATATLGFAVLLFLNNYKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESELTNEIPAPETLDSGAHPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_005194859.3
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194859.3

ORF Insert Sequence:

ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC ACGGGGTCTC CGTCCTGGTG 
CGGTATTTCA TGATCCCGTG TAACGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC 
TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC 
CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TCGGCGCTGT CTGCGAGCCC 
AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCTCCTG CCCCAGCGTG 
GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGAG GCGCATCCGT TTGCTGCGCC GCGGGCCCTG CGGGACCCGG 
GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCTTC TGCGGATGGA 
CTGACAGCCA CGTGCCTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAAGG CCCTGTGTGC 
GGCAGCGACG GCAGTGACTA CCCCAGCGAG TGCCAGCTCT TACGCCACGC CTGTGCCCAC 
CAAGAGAACA TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTTCCTCAGT 
GACCTGAGCC GTACCTGCCG TGTGAACCCA CGCACGCGGC GGCCGGAGAT GCTCCTGCGA 
CCAGAGAGCT GCCCTCCCCG TGGGACACCT GTGTGTGGGG ACGACGGGGT TACTTACGAT 
AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCAAGGCA TCCTCCTGCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC 
CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGCGACCGCG TCGTCTGTGA CGGGGCCTAC 
AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC 
GAGTGTCAGC AGCAGCGTAG CATTCCCATG AAGCACCAGG GCCCGTGTGA CCAGTCCCCA 
TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCAATGT GTGCTGTGAA GAATGGGGAA 
GCCGAATGTG CGTGTCCGAA GGCGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT 
GGCATCACAT ATGGCAGTGC GTGTGAACTG GAAGCCACAG CCTGTGCCCT TGGGCGGGAG 
ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG 
TGTGAAGCTG AGACCGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGCT CTGATGGGCG CACGTATGCC AGTGAATGTG AACTACACGT CCACGCCTGC 
ACACACCAGA TCAGTCTGCA CGTGGCCTCG GCTGGTCCTT GCCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC 
CCTCCACCCG GCCCCGTGTG TGGCAGCGAT GGCGTGACCT ACGGCAGTTC ATGCGAGCTC 
CGTGAGGCTG CCTGTCAGCA GCAAACACAG ATCGAGGAGG CCTGGGCGGG GCCGTGTGAG 
CAAGCTGAGT GTGGCTCCGG AGGCTCGGGC TCGGGAGAGG ACGGCGAGTG TGAGCCTGAG 
CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGACGGGCC ATGCGTCTGC 
GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC 
CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG 
GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCGTTGC CACCTGCGAT TCCCCTGCAC 
TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTTGGC 
CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTACGG CGGCACCTGT 
GACCCTGTCA CAGGTCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ACGGCCGGAG CGGCTGTACC 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA 
TGTTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCC 
CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCCACCCA AGACCTGTGC TGAGATGCTT 
TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA 
CCCACCTGTC CAGGGGCCGA TGCTACCAAG GTCTGCGGGT CAGATGGAGT CACTTATGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAAAGC 
TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCCCGTG TGACATCCGC ATCTGTGGCT 
CCCTCAGCAC TTGACCTGAA CAAGGCACCA TTGCCGCCCC CCAGCGCCTT GCCCCTGGCT 
CCCAGCAGTA CCCGCCACAG CCAGCCCACT TCTCGAGCCT CATCACAACC TTGGACCATG 
GCCAGCATCC CCAGGACCAC TGCAAGGCCA GTGCTGACCA TGCCTCCCGT GGCCCCCTCC 
CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGTGGT 
GACGAGGAGC TGAGTGGTGA CTTGGAGGCC AGTGGGGCTG GCTCAGGAGG ACTTGAGTCC 
CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC CTGCTACAAC 
TCGCCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC 
TGTCCTGCCA CCAAGGTGTT CCAGGGTGTG CTGGAGCTTG AGGGGGTCGA GGGGCAAGAA 
CTGTTCTACA CGCCGGAGAT GGCAGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG 
AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAACTCGG ACGTTAAGAA GGACTTCCGA 
AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC 
TTTGATCCCA CCACAGCCTT CAGGGCGTCT GACGTGGGCC GGGCCCTGCT CCGGCAGCTC 
CAGGCGTCCA GGCGCAGGTC CCTGGGCGTG AGGCGGCCTC TGCAAGAACA TGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACGGCC 
AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC 
AGTCACACAA GCCGGCCCGT CAGCAGGACC ACACCTCCCG CGACCACGCG CCAGCCCCCC 
ACCACTGCCC CTAGTCGTGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC 
CGAAGGCCCT GTGATTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCG 
GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACGGGGG GTGCCGTCTG TGAGAAGGCC 
CTGCACCCCT CCGTGCCAGC CTTTGGGGGC CACTCCTTCC TGGCCTTTCC CACCCTCCGT 
GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG 
CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGTGCTGCT GGGAGGCCGT 
GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACCAGCTC CGTGCCCGTG 
CAGCCCGGCC GCTGGCATCA CCTGGAGCTG TCTCGGCACT GGCGCCAAGG CACTCTCTCC 
GTGGACGGTG AGACTCCTGT TCTGGGCCAG AGCCCCAGTG GCACTGACGG TCTCAACCTG 
GACACTGATC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG 
ACCTCTGTCA GCATCGGTCT TAGGGGTTGT ATCCGCTTGC TGGATGTCAA CAATCAGCGG 
CTGGAGCTGA GCAGCTGGCC AGAGTCTGCG ACTCGAAGCT CCGGAGTGGG CAAGTGCGGG 
GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA 
GGCAGGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGCC CAACCTGTGC CGACGAGAAG 
GACCCTTGTC AGCCGAACCC CTGCCATGGA GCAGCCCCCT GCCGCGTGCT GCCCCAGGGA 
GAGGCCAAGT GCGAGTGCCC CCATGGCCGG GAGGGCAGCC TCTGCCAGAC AGTCTCAGAG 
CCGGAGGACA ACCAGCCGTT CCTGGCTGAT TTCAGCAGCT TCTCCTACCT GGAACTGAAA 
GGCCTGCACA CCTTTGAGCG GGACCTGGGG GAGAAGATGG CACTGGAGGT GGTGTTCCTG 
GCTCGGAGCC CCAGCGGCCT GCTCCTCTAT AATGGACAGA AGACAGATGG CAAGGGCGAC 
TTTGTGTCCC TGGCGCTGCA CAATGGCCTC CTGGAGTTCC GCTACGATCT GGGCAAGGGG 
GCAGCGGTCA TCAGGAGCAA GGAGCCAGTG GCCCTGGGTG CCTGGACCAG GGTCTCCCTG 
GAGCGAAATG GACGCAAAGG AGCCATGCGG GTTGGTGATG GGCCTCGCGT GCTGGGGGAG 
TCCCCGAAAT CCCGCAAGGT GCCACACACC GTTCTCAACC TAAAGGAGCC GCTCTTTGTT 
GGGGGGGCCC CTGACTTCAG CAAGCTGGCC CGAGCAGCTG CTGTGTCCTC TGGCTTTGAT 
GGAGCCATCC AGCTGGTCTC CCTGAATGGC CGCCAACTGC TGACCCGTGA GAACGTGGTC 
CGGGCCGTGG ACGTCTCGTC CTTTGCAGAC CACCCTTGTA CCCAGGCTGA GGGTCAGCCT 
TGCCTCCATG GGGCTTCCTG TCTCCCCCGC GAGGCCTCCT ATGAGTGCCT GTGTCCTGCA 
GGCTTCTCAG GGCTGCACTG TGAGAAGGGC TTGATTGAGA AGTCAGCTGG GGACCTGGAC 
GCACTGGCCT TTGATGGACG GACCTACATC GAGTACCTCA ACGCTGTGAC CGAGAGCGAA 
CTGACCAATG AGATCCCAGC CCCTGAAACT CTGGATTCCG GGGCCCATCC CAGTGAGAAG 
GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG 
CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG 
CGCCTGCAAC TGGCTTATGA CCTGGGCTCC CAGCCCGTGG TGTTGCGCTC CACCGTACCA 
GTGAATACCA ACCGCTGGTT GCGGGTCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT 
CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC 
ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GAGAAGCTGC CCACAGGCCA GGCCCTGCCC 
AAAGCCTATG GCACAGGCTT CGTGGGCTGC CTGCGGGACG TGGTCGTGGG CCAGCGCCCG 
GTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005217206.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217263.1 Bos taurus agrin (AGRN), transcript variant X3, mRNA.

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CloneID	OBa08756
Clone ID Related Accession (Same CDS sequence)	XM_005217206.3 , XM_005194859.3 , XM_005217206.4
Accession Version	XM_005217206.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217206.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC ACGGGGTCTC CGTCCTGGTG 
CGGTATTTCA TGATCCCGTG TAACGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC 
TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC 
CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TCGGCGCTGT CTGCGAGCCC 
AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCTCCTG CCCCAGCGTG 
GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGAG GCGCATCCGT TTGCTGCGCC GCGGGCCCTG CGGGACCCGG 
GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCTTC TGCGGATGGA 
CTGACAGCCA CGTGCCTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAAGG CCCTGTGTGC 
GGCAGCGACG GCAGTGACTA CCCCAGCGAG TGCCAGCTCT TACGCCACGC CTGTGCCCAC 
CAAGAGAACA TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTTCCTCAGT 
GACCTGAGCC GTACCTGCCG TGTGAACCCA CGCACGCGGC GGCCGGAGAT GCTCCTGCGA 
CCAGAGAGCT GCCCTCCCCG TGGGACACCT GTGTGTGGGG ACGACGGGGT TACTTACGAT 
AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCAAGGCA TCCTCCTGCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC 
CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGCGACCGCG TCGTCTGTGA CGGGGCCTAC 
AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC 
GAGTGTCAGC AGCAGCGTAG CATTCCCATG AAGCACCAGG GCCCGTGTGA CCAGTCCCCA 
TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCAATGT GTGCTGTGAA GAATGGGGAA 
GCCGAATGTG CGTGTCCGAA GGCGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT 
GGCATCACAT ATGGCAGTGC GTGTGAACTG GAAGCCACAG CCTGTGCCCT TGGGCGGGAG 
ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG 
TGTGAAGCTG AGACCGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGCT CTGATGGGCG CACGTATGCC AGTGAATGTG AACTACACGT CCACGCCTGC 
ACACACCAGA TCAGTCTGCA CGTGGCCTCG GCTGGTCCTT GCCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC 
CCTCCACCCG GCCCCGTGTG TGGCAGCGAT GGCGTGACCT ACGGCAGTTC ATGCGAGCTC 
CGTGAGGCTG CCTGTCAGCA GCAAACACAG ATCGAGGAGG CCTGGGCGGG GCCGTGTGAG 
CAAGCTGAGT GTGGCTCCGG AGGCTCGGGC TCGGGAGAGG ACGGCGAGTG TGAGCCTGAG 
CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGACGGGCC ATGCGTCTGC 
GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC 
CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG 
GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCGTTGC CACCTGCGAT TCCCCTGCAC 
TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTTGGC 
CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTACGG CGGCACCTGT 
GACCCTGTCA CAGGTCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ACGGCCGGAG CGGCTGTACC 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA 
TGTTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCC 
CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCCACCCA AGACCTGTGC TGAGATGCTT 
TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA 
CCCACCTGTC CAGGGGCCGA TGCTACCAAG GTCTGCGGGT CAGATGGAGT CACTTATGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAAAGC 
TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCCCGTG TGACATCCGC ATCTGTGGCT 
CCCTCAGCAC TTGACCTGAA CAAGGCACCA TTGCCGCCCC CCAGCGCCTT GCCCCTGGCT 
CCCAGCAGTA CCCGCCACAG CCAGCCCACT TCTCGAGCCT CATCACAACC TTGGACCATG 
GCCAGCATCC CCAGGACCAC TGCAAGGCCA GTGCTGACCA TGCCTCCCGT GGCCCCCTCC 
CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGTGGT 
GACGAGGAGC TGAGTGGTGA CTTGGAGGCC AGTGGGGCTG GCTCAGGAGG ACTTGAGTCC 
CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC CTGCTACAAC 
TCGCCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC 
TGTCCTGCCA CCAAGGTGTT CCAGGGTGTG CTGGAGCTTG AGGGGGTCGA GGGGCAAGAA 
CTGTTCTACA CGCCGGAGAT GGCAGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG 
AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAACTCGG ACGTTAAGAA GGACTTCCGA 
AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC 
TTTGATCCCA CCACAGCCTT CAGGGCGTCT GACGTGGGCC GGGCCCTGCT CCGGCAGCTC 
CAGGCGTCCA GGCGCAGGTC CCTGGGCGTG AGGCGGCCTC TGCAAGAACA TGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACGGCC 
AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC 
AGTCACACAA GCCGGCCCGT CAGCAGGACC ACACCTCCCG CGACCACGCG CCAGCCCCCC 
ACCACTGCCC CTAGTCGTGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC 
CGAAGGCCCT GTGATTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCG 
GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACGGGGG GTGCCGTCTG TGAGAAGGCC 
CTGCACCCCT CCGTGCCAGC CTTTGGGGGC CACTCCTTCC TGGCCTTTCC CACCCTCCGT 
GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG 
CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGTGCTGCT GGGAGGCCGT 
GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACCAGCTC CGTGCCCGTG 
CAGCCCGGCC GCTGGCATCA CCTGGAGCTG TCTCGGCACT GGCGCCAAGG CACTCTCTCC 
GTGGACGGTG AGACTCCTGT TCTGGGCCAG AGCCCCAGTG GCACTGACGG TCTCAACCTG 
GACACTGATC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG 
ACCTCTGTCA GCATCGGTCT TAGGGGTTGT ATCCGCTTGC TGGATGTCAA CAATCAGCGG 
CTGGAGCTGA GCAGCTGGCC AGAGTCTGCG ACTCGAAGCT CCGGAGTGGG CAAGTGCGGG 
GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA 
GGCAGGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGCC CAACCTGTGC CGACGAGAAG 
GACCCTTGTC AGCCGAACCC CTGCCATGGA GCAGCCCCCT GCCGCGTGCT GCCCCAGGGA 
GAGGCCAAGT GCGAGTGCCC CCATGGCCGG GAGGGCAGCC TCTGCCAGAC AGTCTCAGAG 
CCGGAGGACA ACCAGCCGTT CCTGGCTGAT TTCAGCAGCT TCTCCTACCT GGAACTGAAA 
GGCCTGCACA CCTTTGAGCG GGACCTGGGG GAGAAGATGG CACTGGAGGT GGTGTTCCTG 
GCTCGGAGCC CCAGCGGCCT GCTCCTCTAT AATGGACAGA AGACAGATGG CAAGGGCGAC 
TTTGTGTCCC TGGCGCTGCA CAATGGCCTC CTGGAGTTCC GCTACGATCT GGGCAAGGGG 
GCAGCGGTCA TCAGGAGCAA GGAGCCAGTG GCCCTGGGTG CCTGGACCAG GGTCTCCCTG 
GAGCGAAATG GACGCAAAGG AGCCATGCGG GTTGGTGATG GGCCTCGCGT GCTGGGGGAG 
TCCCCGAAAT CCCGCAAGGT GCCACACACC GTTCTCAACC TAAAGGAGCC GCTCTTTGTT 
GGGGGGGCCC CTGACTTCAG CAAGCTGGCC CGAGCAGCTG CTGTGTCCTC TGGCTTTGAT 
GGAGCCATCC AGCTGGTCTC CCTGAATGGC CGCCAACTGC TGACCCGTGA GAACGTGGTC 
CGGGCCGTGG ACGTCTCGTC CTTTGCAGAC CACCCTTGTA CCCAGGCTGA GGGTCAGCCT 
TGCCTCCATG GGGCTTCCTG TCTCCCCCGC GAGGCCTCCT ATGAGTGCCT GTGTCCTGCA 
GGCTTCTCAG GGCTGCACTG TGAGAAGGGC TTGATTGAGA AGTCAGCTGG GGACCTGGAC 
GCACTGGCCT TTGATGGACG GACCTACATC GAGTACCTCA ACGCTGTGAC CGAGAGCGAA 
CTGACCAATG AGATCCCAGC CCCTGAAACT CTGGATTCCG GGGCCCATCC CAGTGAGAAG 
GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG 
CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG 
CGCCTGCAAC TGGCTTATGA CCTGGGCTCC CAGCCCGTGG TGTTGCGCTC CACCGTACCA 
GTGAATACCA ACCGCTGGTT GCGGGTCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT 
CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC 
ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GAGAAGCTGC CCACAGGCCA GGCCCTGCCC 
AAAGCCTATG GCACAGGCTT CGTGGGCTGC CTGCGGGACG TGGTCGTGGG CCAGCGCCCG 
GTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217263.1
CDS	27..5906
Translation	MPPLLLERDRRQRHGVSVLVRYFMIPCNVCLILLATATLGFAVLLFLNNYKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESELTNEIPAPETLDSGAHPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_005217206.4
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217206.4

ORF Insert Sequence:

ATGCCTCCAC TGCTGCTGGA GCGGGACCGC CGGCAGCGGC ACGGGGTCTC CGTCCTGGTG 
CGGTATTTCA TGATCCCGTG TAACGTCTGC TTGATCCTCC TGGCCACGGC CACGCTTGGC 
TTCGCGGTGC TACTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC GCCAGTGCCC 
CCAACACCTC CGGATGCGTG CCGAGGAATG CTGTGTGGCT TCGGCGCTGT CTGCGAGCCC 
AGCGCAGACG AGCCGGGCCG AGCCTCCTGC GTGTGCAAGA AGAGCTCCTG CCCCAGCGTG 
GTGGCGCCCG TGTGCGGCTC GGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGAG GCGCATCCGT TTGCTGCGCC GCGGGCCCTG CGGGACCCGG 
GACCCCTGCT CCAATGTCAC CTGCAGCTTT GGCAGCACCT GTGTGCCTTC TGCGGATGGA 
CTGACAGCCA CGTGCCTGTG CCCTGCCACG TGCCTGGGGG CCCCGGAAGG CCCTGTGTGC 
GGCAGCGACG GCAGTGACTA CCCCAGCGAG TGCCAGCTCT TACGCCACGC CTGTGCCCAC 
CAAGAGAACA TCTTCAAGAA GTTCGACGGT CCTTGCGACC CCTGCCAGGG CTTCCTCAGT 
GACCTGAGCC GTACCTGCCG TGTGAACCCA CGCACGCGGC GGCCGGAGAT GCTCCTGCGA 
CCAGAGAGCT GCCCTCCCCG TGGGACACCT GTGTGTGGGG ACGACGGGGT TACTTACGAT 
AATGACTGCG TCATGGGCCG AACAGGGGCT GCTCAAGGCA TCCTCCTGCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC CCGGAGCCGT GCAGATTCAA TGCCGTCTGC 
CTGTCCCGCC GAGGCCGCCC CCGCTGTTCC TGCGACCGCG TCGTCTGTGA CGGGGCCTAC 
AGACCTGTGT GTGCCCATGA CGGGCACACG TATGACAATG ACTGCTGGCG CCAGCAGGCC 
GAGTGTCAGC AGCAGCGTAG CATTCCCATG AAGCACCAGG GCCCGTGTGA CCAGTCCCCA 
TCCCCATGCC GTGGGGTGCA GTGTCCATTT GGGGCAATGT GTGCTGTGAA GAATGGGGAA 
GCCGAATGTG CGTGTCCGAA GGCGTGTTCA GGCGTCTATG ACCCTGTGTG TGGCAGTGAT 
GGCATCACAT ATGGCAGTGC GTGTGAACTG GAAGCCACAG CCTGTGCCCT TGGGCGGGAG 
ATCCGAGTGG CTCGCCGAGG ACCCTGTGAC CGCTGTGGGC AGTGCCGCTT TGGAGCCCTG 
TGTGAAGCTG AGACCGGGCG CTGTGTGTGC CCCTCCGAAT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGCT CTGATGGGCG CACGTATGCC AGTGAATGTG AACTACACGT CCACGCCTGC 
ACACACCAGA TCAGTCTGCA CGTGGCCTCG GCTGGTCCTT GCCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCG GGGCAGTGCG TGTGTCCCCG GTGTGAGCGC 
CCTCCACCCG GCCCCGTGTG TGGCAGCGAT GGCGTGACCT ACGGCAGTTC ATGCGAGCTC 
CGTGAGGCTG CCTGTCAGCA GCAAACACAG ATCGAGGAGG CCTGGGCGGG GCCGTGTGAG 
CAAGCTGAGT GTGGCTCCGG AGGCTCGGGC TCGGGAGAGG ACGGCGAGTG TGAGCCTGAG 
CTGTGCCGGC AGCGAGGTGG CATCTGGGAT GAGGACTCGG AGGACGGGCC ATGCGTCTGC 
GGCTTCAGCT GCCAGGGCGT CCTGAGGAGC CCGGTGTGTG GCTCGGATGG AGTCACCTAC 
CGCACCGAAT GTGAGCTGAA GAAGGCCAGG TGTGAATCAC AGCCAGAGCT GTATGTCGTG 
GCCCAGGGAG CCTGCCGTGG CCCCACTTTG GCCCCGTTGC CACCTGCGAT TCCCCTGCAC 
TGTGCTCAGA CCCCCTACGG TTGCTGCCAG GACAACATCA CTGTTGCCCA GGGTGTTGGC 
CTGGCTGGCT GCCCCAGCTC ATGCCAGTGT AACCCACACG GCTCCTACGG CGGCACCTGT 
GACCCTGTCA CAGGTCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGT ATCGTCACTG ACGGCCGGAG CGGCTGTACC 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGACGACT GTGAGCAGAT GACTGGGCTA 
TGTTCCTGTA AGCCTGGGGT GGCCGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCGTGCC 
CTGGGCCCTG CTGGCTGTGA AACAGACTCT TCGCCACCCA AGACCTGTGC TGAGATGCTT 
TGTGAGTTTG GGGCATCCTG TGTGGAGGAG GCTGGCTCTG CCCACTGCGT GTGCCCAACA 
CCCACCTGTC CAGGGGCCGA TGCTACCAAG GTCTGCGGGT CAGATGGAGT CACTTATGGC 
AATGAGTGCC AGCTGAGGAC CATCGCCTGC CGCCAGGGCT TGGAGATCTC TATCCAAAGC 
TTCGGCCCCT GCCAGGAAGG CATCACTTCT GGTCCCCGTG TGACATCCGC ATCTGTGGCT 
CCCTCAGCAC TTGACCTGAA CAAGGCACCA TTGCCGCCCC CCAGCGCCTT GCCCCTGGCT 
CCCAGCAGTA CCCGCCACAG CCAGCCCACT TCTCGAGCCT CATCACAACC TTGGACCATG 
GCCAGCATCC CCAGGACCAC TGCAAGGCCA GTGCTGACCA TGCCTCCCGT GGCCCCCTCC 
CCGGCAGCCA GCCTCGTCAC CTCTGCCTTC GGCGAATCTG GCAGCGCTGA TGGGAGTGGT 
GACGAGGAGC TGAGTGGTGA CTTGGAGGCC AGTGGGGCTG GCTCAGGAGG ACTTGAGTCC 
CCGGAAAGAG ACAGTGCTGG GACCCCTGGG CTACCCATGG AAAGGGCTTC CTGCTACAAC 
TCGCCTATGG GCTGCTGCTC AGATGGCAAG ACACCCTCCC TGGACGCAGA GGGCTCTAAC 
TGTCCTGCCA CCAAGGTGTT CCAGGGTGTG CTGGAGCTTG AGGGGGTCGA GGGGCAAGAA 
CTGTTCTACA CGCCGGAGAT GGCAGACCCC AAGTCTGAGC TGTTTGGGGA GACTGCCAGG 
AGCATTGAGA GCGCACTGGA TGACCTCTTC CGGAACTCGG ACGTTAAGAA GGACTTCCGA 
AGTGTCCGCC TACGGGACTT GGGGCCAGGC AGCTCGGTCC GGGCCATTGT GGAAGTGCAC 
TTTGATCCCA CCACAGCCTT CAGGGCGTCT GACGTGGGCC GGGCCCTGCT CCGGCAGCTC 
CAGGCGTCCA GGCGCAGGTC CCTGGGCGTG AGGCGGCCTC TGCAAGAACA TGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCATTCTTC ACGGGAGCCA CCCCAGCTGC AGCCACGGCC 
AGAGCCACCA CGGTGGCCCG CCTGCCACCT TCAGCTGCAA CCCCTCGGGC CCACTTTCCC 
AGTCACACAA GCCGGCCCGT CAGCAGGACC ACACCTCCCG CGACCACGCG CCAGCCCCCC 
ACCACTGCCC CTAGTCGTGT GCCTGGACGC CGTCCCCTGC CCCCAGGCAC CCAGCAGCCC 
CGAAGGCCCT GTGATTCCCA GCCCTGCCTC CATGGGGGGA CCTGCCAGGA CCAGGGCTCG 
GGTGCAGACT TCACCTGCAG CTGCCCAGCA GGCACGGGGG GTGCCGTCTG TGAGAAGGCC 
CTGCACCCCT CCGTGCCAGC CTTTGGGGGC CACTCCTTCC TGGCCTTTCC CACCCTCCGT 
GCCTACCACA CCCTGCGCCT GGCGCTCGAG TTCCGGGCGC TGGAGCCCCA GGGGCTGCTG 
CTCTACAACG GCAATGCCCG GGGCAAGGAC TTCCTGGGAC TGGTGCTGCT GGGAGGCCGT 
GTGCAGTTCA GGTTCGACAC AGGCTCAGGG CCCGCGGTGC TGACCAGCTC CGTGCCCGTG 
CAGCCCGGCC GCTGGCATCA CCTGGAGCTG TCTCGGCACT GGCGCCAAGG CACTCTCTCC 
GTGGACGGTG AGACTCCTGT TCTGGGCCAG AGCCCCAGTG GCACTGACGG TCTCAACCTG 
GACACTGATC TTTTCGTGGG TGGCGTCCCT GAGGATCAGG CTTCCACGGT GCTAGAGCGG 
ACCTCTGTCA GCATCGGTCT TAGGGGTTGT ATCCGCTTGC TGGATGTCAA CAATCAGCGG 
CTGGAGCTGA GCAGCTGGCC AGAGTCTGCG ACTCGAAGCT CCGGAGTGGG CAAGTGCGGG 
GACCACCCCT GTCTGCCTAG CCCCTGCCTC GGAGGGGCCC CGTGCCAGGC CCTGGAGGCA 
GGCAGGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGCC CAACCTGTGC CGACGAGAAG 
GACCCTTGTC AGCCGAACCC CTGCCATGGA GCAGCCCCCT GCCGCGTGCT GCCCCAGGGA 
GAGGCCAAGT GCGAGTGCCC CCATGGCCGG GAGGGCAGCC TCTGCCAGAC AGTCTCAGAG 
CCGGAGGACA ACCAGCCGTT CCTGGCTGAT TTCAGCAGCT TCTCCTACCT GGAACTGAAA 
GGCCTGCACA CCTTTGAGCG GGACCTGGGG GAGAAGATGG CACTGGAGGT GGTGTTCCTG 
GCTCGGAGCC CCAGCGGCCT GCTCCTCTAT AATGGACAGA AGACAGATGG CAAGGGCGAC 
TTTGTGTCCC TGGCGCTGCA CAATGGCCTC CTGGAGTTCC GCTACGATCT GGGCAAGGGG 
GCAGCGGTCA TCAGGAGCAA GGAGCCAGTG GCCCTGGGTG CCTGGACCAG GGTCTCCCTG 
GAGCGAAATG GACGCAAAGG AGCCATGCGG GTTGGTGATG GGCCTCGCGT GCTGGGGGAG 
TCCCCGAAAT CCCGCAAGGT GCCACACACC GTTCTCAACC TAAAGGAGCC GCTCTTTGTT 
GGGGGGGCCC CTGACTTCAG CAAGCTGGCC CGAGCAGCTG CTGTGTCCTC TGGCTTTGAT 
GGAGCCATCC AGCTGGTCTC CCTGAATGGC CGCCAACTGC TGACCCGTGA GAACGTGGTC 
CGGGCCGTGG ACGTCTCGTC CTTTGCAGAC CACCCTTGTA CCCAGGCTGA GGGTCAGCCT 
TGCCTCCATG GGGCTTCCTG TCTCCCCCGC GAGGCCTCCT ATGAGTGCCT GTGTCCTGCA 
GGCTTCTCAG GGCTGCACTG TGAGAAGGGC TTGATTGAGA AGTCAGCTGG GGACCTGGAC 
GCACTGGCCT TTGATGGACG GACCTACATC GAGTACCTCA ACGCTGTGAC CGAGAGCGAA 
CTGACCAATG AGATCCCAGC CCCTGAAACT CTGGATTCCG GGGCCCATCC CAGTGAGAAG 
GCACTCCAGA GCAACCACTT TGAGCTGAGC CTGCGCACTG AGGCCACACA GGGGCTGGTG 
CTGTGGAGTG GCAAGGCCAC GGAGCGGGCT GACTACATCG CACTGGCCAT CGTGGACGGG 
CGCCTGCAAC TGGCTTATGA CCTGGGCTCC CAGCCCGTGG TGTTGCGCTC CACCGTACCA 
GTGAATACCA ACCGCTGGTT GCGGGTCAGG GCACACAGGA AACAGAGGGA AGGTTCCCTT 
CAGGTGGGCA ACGAGGCCCC TGTGACTGGC TCCTCCCCAC TGGGTGCCAC GCAGCTGGAC 
ACAGATGGAG CCCTGTGGCT GGGTGGCCTG GAGAAGCTGC CCACAGGCCA GGCCCTGCCC 
AAAGCCTATG GCACAGGCTT CGTGGGCTGC CTGCGGGACG TGGTCGTGGG CCAGCGCCCG 
GTGCACCTGC TGGAGGACGC CATCACCAAG CCAGAGCTTC GGCCCTGCCC TGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005217205.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217262.1 Bos taurus agrin (AGRN), transcript variant X1, mRNA.

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CloneID	OBa08754
Clone ID Related Accession (Same CDS sequence)	XM_005217205.3 , XM_005194858.3 , XM_005217205.4
Accession Version	XM_005217205.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217205.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGAAA TCCCGCAAGG TGCCACACAC CGTTCTCAAC 
CTAAAGGAGC CGCTCTTTGT TGGGGGGGCC CCTGACTTCA GCAAGCTGGC CCGAGCAGCT 
GCTGTGTCCT CTGGCTTTGA TGGAGCCATC CAGCTGGTCT CCCTGAATGG CCGCCAACTG 
CTGACCCGTG AGAACGTGGT CCGGGCCGTG GACGTCTCGT CCTTTGCAGA CCACCCTTGT 
ACCCAGGCTG AGGGTCAGCC TTGCCTCCAT GGGGCTTCCT GTCTCCCCCG CGAGGCCTCC 
TATGAGTGCC TGTGTCCTGC AGGCTTCTCA GGGCTGCACT GTGAGAAGGG CTTGATTGAG 
AAGTCAGCTG GGGACCTGGA CGCACTGGCC TTTGATGGAC GGACCTACAT CGAGTACCTC 
AACGCTGTGA CCGAGAGCGA ACTGACCAAT GAGATCCCAG CCCCTGAAAC TCTGGATTCC 
GGGGCCCATC CCAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT 
GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC 
GCACTGGCCA TCGTGGACGG GCGCCTGCAA CTGGCTTATG ACCTGGGCTC CCAGCCCGTG 
GTGTTGCGCT CCACCGTACC AGTGAATACC AACCGCTGGT TGCGGGTCAG GGCACACAGG 
AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA 
CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGAGAAGCTG 
CCCACAGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TCGTGGGCTG CCTGCGGGAC 
GTGGTCGTGG GCCAGCGCCC GGTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT 
CGGCCCTGCC CTGCCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217262.1
CDS	127..6327
Translation	MASFRPFSRALLQPLLLLLVVAVRALPSADGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESELTNEIPAPETLDSGAHPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_005217205.3
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217205.3

ORF Insert Sequence:

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGAAA TCCCGCAAGG TGCCACACAC CGTTCTCAAC 
CTAAAGGAGC CGCTCTTTGT TGGGGGGGCC CCTGACTTCA GCAAGCTGGC CCGAGCAGCT 
GCTGTGTCCT CTGGCTTTGA TGGAGCCATC CAGCTGGTCT CCCTGAATGG CCGCCAACTG 
CTGACCCGTG AGAACGTGGT CCGGGCCGTG GACGTCTCGT CCTTTGCAGA CCACCCTTGT 
ACCCAGGCTG AGGGTCAGCC TTGCCTCCAT GGGGCTTCCT GTCTCCCCCG CGAGGCCTCC 
TATGAGTGCC TGTGTCCTGC AGGCTTCTCA GGGCTGCACT GTGAGAAGGG CTTGATTGAG 
AAGTCAGCTG GGGACCTGGA CGCACTGGCC TTTGATGGAC GGACCTACAT CGAGTACCTC 
AACGCTGTGA CCGAGAGCGA ACTGACCAAT GAGATCCCAG CCCCTGAAAC TCTGGATTCC 
GGGGCCCATC CCAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT 
GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC 
GCACTGGCCA TCGTGGACGG GCGCCTGCAA CTGGCTTATG ACCTGGGCTC CCAGCCCGTG 
GTGTTGCGCT CCACCGTACC AGTGAATACC AACCGCTGGT TGCGGGTCAG GGCACACAGG 
AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA 
CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGAGAAGCTG 
CCCACAGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TCGTGGGCTG CCTGCGGGAC 
GTGGTCGTGG GCCAGCGCCC GGTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT 
CGGCCCTGCC CTGCCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005194858.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194915.1 Bos taurus agrin (AGRN), transcript variant X1, mRNA.

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CloneID	OBa08754
Clone ID Related Accession (Same CDS sequence)	XM_005217205.3 , XM_005194858.3 , XM_005217205.4
Accession Version	XM_005194858.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194858.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGAAA TCCCGCAAGG TGCCACACAC CGTTCTCAAC 
CTAAAGGAGC CGCTCTTTGT TGGGGGGGCC CCTGACTTCA GCAAGCTGGC CCGAGCAGCT 
GCTGTGTCCT CTGGCTTTGA TGGAGCCATC CAGCTGGTCT CCCTGAATGG CCGCCAACTG 
CTGACCCGTG AGAACGTGGT CCGGGCCGTG GACGTCTCGT CCTTTGCAGA CCACCCTTGT 
ACCCAGGCTG AGGGTCAGCC TTGCCTCCAT GGGGCTTCCT GTCTCCCCCG CGAGGCCTCC 
TATGAGTGCC TGTGTCCTGC AGGCTTCTCA GGGCTGCACT GTGAGAAGGG CTTGATTGAG 
AAGTCAGCTG GGGACCTGGA CGCACTGGCC TTTGATGGAC GGACCTACAT CGAGTACCTC 
AACGCTGTGA CCGAGAGCGA ACTGACCAAT GAGATCCCAG CCCCTGAAAC TCTGGATTCC 
GGGGCCCATC CCAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT 
GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC 
GCACTGGCCA TCGTGGACGG GCGCCTGCAA CTGGCTTATG ACCTGGGCTC CCAGCCCGTG 
GTGTTGCGCT CCACCGTACC AGTGAATACC AACCGCTGGT TGCGGGTCAG GGCACACAGG 
AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA 
CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGAGAAGCTG 
CCCACAGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TCGTGGGCTG CCTGCGGGAC 
GTGGTCGTGG GCCAGCGCCC GGTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT 
CGGCCCTGCC CTGCCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194915.1
CDS	127..6327
Translation	MASFRPFSRALLQPLLLLLVVAVRALPSADGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESELTNEIPAPETLDSGAHPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_005194858.3
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194858.3

ORF Insert Sequence:

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGAAA TCCCGCAAGG TGCCACACAC CGTTCTCAAC 
CTAAAGGAGC CGCTCTTTGT TGGGGGGGCC CCTGACTTCA GCAAGCTGGC CCGAGCAGCT 
GCTGTGTCCT CTGGCTTTGA TGGAGCCATC CAGCTGGTCT CCCTGAATGG CCGCCAACTG 
CTGACCCGTG AGAACGTGGT CCGGGCCGTG GACGTCTCGT CCTTTGCAGA CCACCCTTGT 
ACCCAGGCTG AGGGTCAGCC TTGCCTCCAT GGGGCTTCCT GTCTCCCCCG CGAGGCCTCC 
TATGAGTGCC TGTGTCCTGC AGGCTTCTCA GGGCTGCACT GTGAGAAGGG CTTGATTGAG 
AAGTCAGCTG GGGACCTGGA CGCACTGGCC TTTGATGGAC GGACCTACAT CGAGTACCTC 
AACGCTGTGA CCGAGAGCGA ACTGACCAAT GAGATCCCAG CCCCTGAAAC TCTGGATTCC 
GGGGCCCATC CCAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT 
GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC 
GCACTGGCCA TCGTGGACGG GCGCCTGCAA CTGGCTTATG ACCTGGGCTC CCAGCCCGTG 
GTGTTGCGCT CCACCGTACC AGTGAATACC AACCGCTGGT TGCGGGTCAG GGCACACAGG 
AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA 
CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGAGAAGCTG 
CCCACAGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TCGTGGGCTG CCTGCGGGAC 
GTGGTCGTGG GCCAGCGCCC GGTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT 
CGGCCCTGCC CTGCCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005217205.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217262.1 Bos taurus agrin (AGRN), transcript variant X1, mRNA.

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CloneID	OBa08754
Clone ID Related Accession (Same CDS sequence)	XM_005217205.3 , XM_005194858.3 , XM_005217205.4
Accession Version	XM_005217205.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217205.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGAAA TCCCGCAAGG TGCCACACAC CGTTCTCAAC 
CTAAAGGAGC CGCTCTTTGT TGGGGGGGCC CCTGACTTCA GCAAGCTGGC CCGAGCAGCT 
GCTGTGTCCT CTGGCTTTGA TGGAGCCATC CAGCTGGTCT CCCTGAATGG CCGCCAACTG 
CTGACCCGTG AGAACGTGGT CCGGGCCGTG GACGTCTCGT CCTTTGCAGA CCACCCTTGT 
ACCCAGGCTG AGGGTCAGCC TTGCCTCCAT GGGGCTTCCT GTCTCCCCCG CGAGGCCTCC 
TATGAGTGCC TGTGTCCTGC AGGCTTCTCA GGGCTGCACT GTGAGAAGGG CTTGATTGAG 
AAGTCAGCTG GGGACCTGGA CGCACTGGCC TTTGATGGAC GGACCTACAT CGAGTACCTC 
AACGCTGTGA CCGAGAGCGA ACTGACCAAT GAGATCCCAG CCCCTGAAAC TCTGGATTCC 
GGGGCCCATC CCAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT 
GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC 
GCACTGGCCA TCGTGGACGG GCGCCTGCAA CTGGCTTATG ACCTGGGCTC CCAGCCCGTG 
GTGTTGCGCT CCACCGTACC AGTGAATACC AACCGCTGGT TGCGGGTCAG GGCACACAGG 
AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA 
CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGAGAAGCTG 
CCCACAGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TCGTGGGCTG CCTGCGGGAC 
GTGGTCGTGG GCCAGCGCCC GGTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT 
CGGCCCTGCC CTGCCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217262.1
CDS	123..6323
Translation	MASFRPFSRALLQPLLLLLVVAVRALPSADGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESELTNEIPAPETLDSGAHPSEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_005217205.4
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217205.4

ORF Insert Sequence:

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGAAA TCCCGCAAGG TGCCACACAC CGTTCTCAAC 
CTAAAGGAGC CGCTCTTTGT TGGGGGGGCC CCTGACTTCA GCAAGCTGGC CCGAGCAGCT 
GCTGTGTCCT CTGGCTTTGA TGGAGCCATC CAGCTGGTCT CCCTGAATGG CCGCCAACTG 
CTGACCCGTG AGAACGTGGT CCGGGCCGTG GACGTCTCGT CCTTTGCAGA CCACCCTTGT 
ACCCAGGCTG AGGGTCAGCC TTGCCTCCAT GGGGCTTCCT GTCTCCCCCG CGAGGCCTCC 
TATGAGTGCC TGTGTCCTGC AGGCTTCTCA GGGCTGCACT GTGAGAAGGG CTTGATTGAG 
AAGTCAGCTG GGGACCTGGA CGCACTGGCC TTTGATGGAC GGACCTACAT CGAGTACCTC 
AACGCTGTGA CCGAGAGCGA ACTGACCAAT GAGATCCCAG CCCCTGAAAC TCTGGATTCC 
GGGGCCCATC CCAGTGAGAA GGCACTCCAG AGCAACCACT TTGAGCTGAG CCTGCGCACT 
GAGGCCACAC AGGGGCTGGT GCTGTGGAGT GGCAAGGCCA CGGAGCGGGC TGACTACATC 
GCACTGGCCA TCGTGGACGG GCGCCTGCAA CTGGCTTATG ACCTGGGCTC CCAGCCCGTG 
GTGTTGCGCT CCACCGTACC AGTGAATACC AACCGCTGGT TGCGGGTCAG GGCACACAGG 
AAACAGAGGG AAGGTTCCCT TCAGGTGGGC AACGAGGCCC CTGTGACTGG CTCCTCCCCA 
CTGGGTGCCA CGCAGCTGGA CACAGATGGA GCCCTGTGGC TGGGTGGCCT GGAGAAGCTG 
CCCACAGGCC AGGCCCTGCC CAAAGCCTAT GGCACAGGCT TCGTGGGCTG CCTGCGGGAC 
GTGGTCGTGG GCCAGCGCCC GGTGCACCTG CTGGAGGACG CCATCACCAA GCCAGAGCTT 
CGGCCCTGCC CTGCCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_002694193.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002694239.1 Bos taurus agrin (AGRN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa08755
Clone ID Related Accession (Same CDS sequence)	XM_002694193.4 , XM_002694193.5 , XM_604151.8
Accession Version	XM_002694193.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694193.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGGTG CCACACACCG TTCTCAACCT AAAGGAGCCG 
CTCTTTGTTG GGGGGGCCCC TGACTTCAGC AAGCTGGCCC GAGCAGCTGC TGTGTCCTCT 
GGCTTTGATG GAGCCATCCA GCTGGTCTCC CTGAATGGCC GCCAACTGCT GACCCGTGAG 
AACGTGGTCC GGGCCGTGGA CGTCTCGTCC TTTGCAGACC ACCCTTGTAC CCAGGCTGAG 
GGTCAGCCTT GCCTCCATGG GGCTTCCTGT CTCCCCCGCG AGGCCTCCTA TGAGTGCCTG 
TGTCCTGCAG GCTTCTCAGG GCTGCACTGT GAGAAGGGCT TGATTGAGAA GTCAGCTGGG 
GACCTGGACG CACTGGCCTT TGATGGACGG ACCTACATCG AGTACCTCAA CGCTGTGACC 
GAGAGTGAGA AGGCACTCCA GAGCAACCAC TTTGAGCTGA GCCTGCGCAC TGAGGCCACA 
CAGGGGCTGG TGCTGTGGAG TGGCAAGGCC ACGGAGCGGG CTGACTACAT CGCACTGGCC 
ATCGTGGACG GGCGCCTGCA ACTGGCTTAT GACCTGGGCT CCCAGCCCGT GGTGTTGCGC 
TCCACCGTAC CAGTGAATAC CAACCGCTGG TTGCGGGTCA GGGCACACAG GAAACAGAGG 
GAAGGTTCCC TTCAGGTGGG CAACGAGGCC CCTGTGACTG GCTCCTCCCC ACTGGGTGCC 
ACGCAGCTGG ACACAGATGG AGCCCTGTGG CTGGGTGGCC TGGAGAAGCT GCCCACAGGC 
CAGGCCCTGC CCAAAGCCTA TGGCACAGGC TTCGTGGGCT GCCTGCGGGA CGTGGTCGTG 
GGCCAGCGCC CGGTGCACCT GCTGGAGGAC GCCATCACCA AGCCAGAGCT TCGGCCCTGC 
CCTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002694239.1
CDS	125..6256
Translation	MASFRPFSRALLQPLLLLLVVAVRALPSADGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_002694193.4
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002694193.4

ORF Insert Sequence:

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGGTG CCACACACCG TTCTCAACCT AAAGGAGCCG 
CTCTTTGTTG GGGGGGCCCC TGACTTCAGC AAGCTGGCCC GAGCAGCTGC TGTGTCCTCT 
GGCTTTGATG GAGCCATCCA GCTGGTCTCC CTGAATGGCC GCCAACTGCT GACCCGTGAG 
AACGTGGTCC GGGCCGTGGA CGTCTCGTCC TTTGCAGACC ACCCTTGTAC CCAGGCTGAG 
GGTCAGCCTT GCCTCCATGG GGCTTCCTGT CTCCCCCGCG AGGCCTCCTA TGAGTGCCTG 
TGTCCTGCAG GCTTCTCAGG GCTGCACTGT GAGAAGGGCT TGATTGAGAA GTCAGCTGGG 
GACCTGGACG CACTGGCCTT TGATGGACGG ACCTACATCG AGTACCTCAA CGCTGTGACC 
GAGAGTGAGA AGGCACTCCA GAGCAACCAC TTTGAGCTGA GCCTGCGCAC TGAGGCCACA 
CAGGGGCTGG TGCTGTGGAG TGGCAAGGCC ACGGAGCGGG CTGACTACAT CGCACTGGCC 
ATCGTGGACG GGCGCCTGCA ACTGGCTTAT GACCTGGGCT CCCAGCCCGT GGTGTTGCGC 
TCCACCGTAC CAGTGAATAC CAACCGCTGG TTGCGGGTCA GGGCACACAG GAAACAGAGG 
GAAGGTTCCC TTCAGGTGGG CAACGAGGCC CCTGTGACTG GCTCCTCCCC ACTGGGTGCC 
ACGCAGCTGG ACACAGATGG AGCCCTGTGG CTGGGTGGCC TGGAGAAGCT GCCCACAGGC 
CAGGCCCTGC CCAAAGCCTA TGGCACAGGC TTCGTGGGCT GCCTGCGGGA CGTGGTCGTG 
GGCCAGCGCC CGGTGCACCT GCTGGAGGAC GCCATCACCA AGCCAGAGCT TCGGCCCTGC 
CCTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_002694193.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002694239.1 Bos taurus agrin (AGRN), transcript variant X2, mRNA.

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CloneID	OBa08755
Clone ID Related Accession (Same CDS sequence)	XM_002694193.4 , XM_002694193.5 , XM_604151.8
Accession Version	XM_002694193.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694193.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGGTG CCACACACCG TTCTCAACCT AAAGGAGCCG 
CTCTTTGTTG GGGGGGCCCC TGACTTCAGC AAGCTGGCCC GAGCAGCTGC TGTGTCCTCT 
GGCTTTGATG GAGCCATCCA GCTGGTCTCC CTGAATGGCC GCCAACTGCT GACCCGTGAG 
AACGTGGTCC GGGCCGTGGA CGTCTCGTCC TTTGCAGACC ACCCTTGTAC CCAGGCTGAG 
GGTCAGCCTT GCCTCCATGG GGCTTCCTGT CTCCCCCGCG AGGCCTCCTA TGAGTGCCTG 
TGTCCTGCAG GCTTCTCAGG GCTGCACTGT GAGAAGGGCT TGATTGAGAA GTCAGCTGGG 
GACCTGGACG CACTGGCCTT TGATGGACGG ACCTACATCG AGTACCTCAA CGCTGTGACC 
GAGAGTGAGA AGGCACTCCA GAGCAACCAC TTTGAGCTGA GCCTGCGCAC TGAGGCCACA 
CAGGGGCTGG TGCTGTGGAG TGGCAAGGCC ACGGAGCGGG CTGACTACAT CGCACTGGCC 
ATCGTGGACG GGCGCCTGCA ACTGGCTTAT GACCTGGGCT CCCAGCCCGT GGTGTTGCGC 
TCCACCGTAC CAGTGAATAC CAACCGCTGG TTGCGGGTCA GGGCACACAG GAAACAGAGG 
GAAGGTTCCC TTCAGGTGGG CAACGAGGCC CCTGTGACTG GCTCCTCCCC ACTGGGTGCC 
ACGCAGCTGG ACACAGATGG AGCCCTGTGG CTGGGTGGCC TGGAGAAGCT GCCCACAGGC 
CAGGCCCTGC CCAAAGCCTA TGGCACAGGC TTCGTGGGCT GCCTGCGGGA CGTGGTCGTG 
GGCCAGCGCC CGGTGCACCT GCTGGAGGAC GCCATCACCA AGCCAGAGCT TCGGCCCTGC 
CCTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002694239.1
CDS	89..6220
Translation	MASFRPFSRALLQPLLLLLVVAVRALPSADGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_002694193.5
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002694193.5

ORF Insert Sequence:

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGGTG CCACACACCG TTCTCAACCT AAAGGAGCCG 
CTCTTTGTTG GGGGGGCCCC TGACTTCAGC AAGCTGGCCC GAGCAGCTGC TGTGTCCTCT 
GGCTTTGATG GAGCCATCCA GCTGGTCTCC CTGAATGGCC GCCAACTGCT GACCCGTGAG 
AACGTGGTCC GGGCCGTGGA CGTCTCGTCC TTTGCAGACC ACCCTTGTAC CCAGGCTGAG 
GGTCAGCCTT GCCTCCATGG GGCTTCCTGT CTCCCCCGCG AGGCCTCCTA TGAGTGCCTG 
TGTCCTGCAG GCTTCTCAGG GCTGCACTGT GAGAAGGGCT TGATTGAGAA GTCAGCTGGG 
GACCTGGACG CACTGGCCTT TGATGGACGG ACCTACATCG AGTACCTCAA CGCTGTGACC 
GAGAGTGAGA AGGCACTCCA GAGCAACCAC TTTGAGCTGA GCCTGCGCAC TGAGGCCACA 
CAGGGGCTGG TGCTGTGGAG TGGCAAGGCC ACGGAGCGGG CTGACTACAT CGCACTGGCC 
ATCGTGGACG GGCGCCTGCA ACTGGCTTAT GACCTGGGCT CCCAGCCCGT GGTGTTGCGC 
TCCACCGTAC CAGTGAATAC CAACCGCTGG TTGCGGGTCA GGGCACACAG GAAACAGAGG 
GAAGGTTCCC TTCAGGTGGG CAACGAGGCC CCTGTGACTG GCTCCTCCCC ACTGGGTGCC 
ACGCAGCTGG ACACAGATGG AGCCCTGTGG CTGGGTGGCC TGGAGAAGCT GCCCACAGGC 
CAGGCCCTGC CCAAAGCCTA TGGCACAGGC TTCGTGGGCT GCCTGCGGGA CGTGGTCGTG 
GGCCAGCGCC CGGTGCACCT GCTGGAGGAC GCCATCACCA AGCCAGAGCT TCGGCCCTGC 
CCTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_604151.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_604151.4 Bos taurus agrin (AGRN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa08755
Clone ID Related Accession (Same CDS sequence)	XM_002694193.4 , XM_002694193.5 , XM_604151.8
Accession Version	XM_604151.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_604151.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGGTG CCACACACCG TTCTCAACCT AAAGGAGCCG 
CTCTTTGTTG GGGGGGCCCC TGACTTCAGC AAGCTGGCCC GAGCAGCTGC TGTGTCCTCT 
GGCTTTGATG GAGCCATCCA GCTGGTCTCC CTGAATGGCC GCCAACTGCT GACCCGTGAG 
AACGTGGTCC GGGCCGTGGA CGTCTCGTCC TTTGCAGACC ACCCTTGTAC CCAGGCTGAG 
GGTCAGCCTT GCCTCCATGG GGCTTCCTGT CTCCCCCGCG AGGCCTCCTA TGAGTGCCTG 
TGTCCTGCAG GCTTCTCAGG GCTGCACTGT GAGAAGGGCT TGATTGAGAA GTCAGCTGGG 
GACCTGGACG CACTGGCCTT TGATGGACGG ACCTACATCG AGTACCTCAA CGCTGTGACC 
GAGAGTGAGA AGGCACTCCA GAGCAACCAC TTTGAGCTGA GCCTGCGCAC TGAGGCCACA 
CAGGGGCTGG TGCTGTGGAG TGGCAAGGCC ACGGAGCGGG CTGACTACAT CGCACTGGCC 
ATCGTGGACG GGCGCCTGCA ACTGGCTTAT GACCTGGGCT CCCAGCCCGT GGTGTTGCGC 
TCCACCGTAC CAGTGAATAC CAACCGCTGG TTGCGGGTCA GGGCACACAG GAAACAGAGG 
GAAGGTTCCC TTCAGGTGGG CAACGAGGCC CCTGTGACTG GCTCCTCCCC ACTGGGTGCC 
ACGCAGCTGG ACACAGATGG AGCCCTGTGG CTGGGTGGCC TGGAGAAGCT GCCCACAGGC 
CAGGCCCTGC CCAAAGCCTA TGGCACAGGC TTCGTGGGCT GCCTGCGGGA CGTGGTCGTG 
GGCCAGCGCC CGGTGCACCT GCTGGAGGAC GCCATCACCA AGCCAGAGCT TCGGCCCTGC 
CCTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_604151.4
CDS	125..6256
Translation	MASFRPFSRALLQPLLLLLVVAVRALPSADGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEHCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPSADEPGRASCVCKKSSCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLRRGPCGTRDPCSNVTCSFGSTCVPSADGLTATCLCPATCLGAPEGPVCGSDGSDYPSECQLLRHACAHQENIFKKFDGPCDPCQGFLSDLSRTCRVNPRTRRPEMLLRPESCPPRGTPVCGDDGVTYDNDCVMGRTGAAQGILLQKVRSGQCQPRDQCPEPCRFNAVCLSRRGRPRCSCDRVVCDGAYRPVCAHDGHTYDNDCWRQQAECQQQRSIPMKHQGPCDQSPSPCRGVQCPFGAMCAVKNGEAECACPKACSGVYDPVCGSDGITYGSACELEATACALGREIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGRTYASECELHVHACTHQISLHVASAGPCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYGSSCELREAACQQQTQIEEAWAGPCEQAECGSGGSGSGEDGECEPELCRQRGGIWDEDSEDGPCVCGFSCQGVLRSPVCGSDGVTYRTECELKKARCESQPELYVVAQGACRGPTLAPLPPAIPLHCAQTPYGCCQDNITVAQGVGLAGCPSSCQCNPHGSYGGTCDPVTGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCETDSSPPKTCAEMLCEFGASCVEEAGSAHCVCPTPTCPGADATKVCGSDGVTYGNECQLRTIACRQGLEISIQSFGPCQEGITSGPRVTSASVAPSALDLNKAPLPPPSALPLAPSSTRHSQPTSRASSQPWTMASIPRTTARPVLTMPPVAPSPAASLVTSAFGESGSADGSGDEELSGDLEASGAGSGGLESPERDSAGTPGLPMERASCYNSPMGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESALDDLFRNSDVKKDFRSVRLRDLGPGSSVRAIVEVHFDPTTAFRASDVGRALLRQLQASRRRSLGVRRPLQEHVRFMDFDWFPAFFTGATPAAATARATTVARLPPSAATPRAHFPSHTSRPVSRTTPPATTRQPPTTAPSRVPGRRPLPPGTQQPRRPCDSQPCLHGGTCQDQGSGADFTCSCPAGTGGAVCEKALHPSVPAFGGHSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLGLVLLGGRVQFRFDTGSGPAVLTSSVPVQPGRWHHLELSRHWRQGTLSVDGETPVLGQSPSGTDGLNLDTDLFVGGVPEDQASTVLERTSVSIGLRGCIRLLDVNNQRLELSSWPESATRSSGVGKCGDHPCLPSPCLGGAPCQALEAGRFHCQCPPGRFGPTCADEKDPCQPNPCHGAAPCRVLPQGEAKCECPHGREGSLCQTVSEPEDNQPFLADFSSFSYLELKGLHTFERDLGEKMALEVVFLARSPSGLLLYNGQKTDGKGDFVSLALHNGLLEFRYDLGKGAAVIRSKEPVALGAWTRVSLERNGRKGAMRVGDGPRVLGESPVPHTVLNLKEPLFVGGAPDFSKLARAAAVSSGFDGAIQLVSLNGRQLLTRENVVRAVDVSSFADHPCTQAEGQPCLHGASCLPREASYECLCPAGFSGLHCEKGLIEKSAGDLDALAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYIALAIVDGRLQLAYDLGSQPVVLRSTVPVNTNRWLRVRAHRKQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLEKLPTGQALPKAYGTGFVGCLRDVVVGQRPVHLLEDAITKPELRPCPAL

Target ORF information:

RefSeq Version	XM_604151.8
Organism	Bos taurus(cattle)
Definition	Bos taurus agrin (AGRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_604151.8

ORF Insert Sequence:

ATGGCCAGCT TTCGGCCGTT CAGCCGGGCC CTGCTGCAGC CTTTGCTGCT GCTCCTGGTG 
GTGGCCGTGC GCGCCCTGCC CAGCGCCGAC GGGACGTGCC CCGAACGCGC GCTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACCGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACATACTC GTGCAAGGTT CGGGTCTGGC GGTACCTGAA GGGCAAAGAT 
GTGGTGGCCC AGGAAAGCCT GCTGGACGGA GGCAACAAAG TGGTGATTGG CGGCTTCGGA 
GACCCCCTCA TCTGTGACAA CCAGGTGTCC ACTGGAGACA CCAGGATCTT CTTTGTGAAC 
CCTGCCCCGC CATACCTGTG GCCCGCCCAC AAGAATGAGC TGATGCTAAA CTCCAGCCTC 
ATGCGCATCA CCCTGCGGAA CCTGGAGGAG GTCGAGCACT GTGTGGAAGA CAAACCTGGG 
ACCCACTTCA CGCCAGTGCC CCCAACACCT CCGGATGCGT GCCGAGGAAT GCTGTGTGGC 
TTCGGCGCTG TCTGCGAGCC CAGCGCAGAC GAGCCGGGCC GAGCCTCCTG CGTGTGCAAG 
AAGAGCTCCT GCCCCAGCGT GGTGGCGCCC GTGTGCGGCT CGGATGCCTC CACCTACAGC 
AACGAGTGCG AGCTGCAGCG GGCGCAGTGC AGCCAGCAGA GGCGCATCCG TTTGCTGCGC 
CGCGGGCCCT GCGGGACCCG GGACCCCTGC TCCAATGTCA CCTGCAGCTT TGGCAGCACC 
TGTGTGCCTT CTGCGGATGG ACTGACAGCC ACGTGCCTGT GCCCTGCCAC GTGCCTGGGG 
GCCCCGGAAG GCCCTGTGTG CGGCAGCGAC GGCAGTGACT ACCCCAGCGA GTGCCAGCTC 
TTACGCCACG CCTGTGCCCA CCAAGAGAAC ATCTTCAAGA AGTTCGACGG TCCTTGCGAC 
CCCTGCCAGG GCTTCCTCAG TGACCTGAGC CGTACCTGCC GTGTGAACCC ACGCACGCGG 
CGGCCGGAGA TGCTCCTGCG ACCAGAGAGC TGCCCTCCCC GTGGGACACC TGTGTGTGGG 
GACGACGGGG TTACTTACGA TAATGACTGC GTCATGGGCC GAACAGGGGC TGCTCAAGGC 
ATCCTCCTGC AGAAAGTGCG CTCTGGCCAG TGCCAGCCTC GAGACCAGTG CCCGGAGCCG 
TGCAGATTCA ATGCCGTCTG CCTGTCCCGC CGAGGCCGCC CCCGCTGTTC CTGCGACCGC 
GTCGTCTGTG ACGGGGCCTA CAGACCTGTG TGTGCCCATG ACGGGCACAC GTATGACAAT 
GACTGCTGGC GCCAGCAGGC CGAGTGTCAG CAGCAGCGTA GCATTCCCAT GAAGCACCAG 
GGCCCGTGTG ACCAGTCCCC ATCCCCATGC CGTGGGGTGC AGTGTCCATT TGGGGCAATG 
TGTGCTGTGA AGAATGGGGA AGCCGAATGT GCGTGTCCGA AGGCGTGTTC AGGCGTCTAT 
GACCCTGTGT GTGGCAGTGA TGGCATCACA TATGGCAGTG CGTGTGAACT GGAAGCCACA 
GCCTGTGCCC TTGGGCGGGA GATCCGAGTG GCTCGCCGAG GACCCTGTGA CCGCTGTGGG 
CAGTGCCGCT TTGGAGCCCT GTGTGAAGCT GAGACCGGGC GCTGTGTGTG CCCCTCCGAA 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGC TCTGATGGGC GCACGTATGC CAGTGAATGT 
GAACTACACG TCCACGCCTG CACACACCAG ATCAGTCTGC ACGTGGCCTC GGCTGGTCCT 
TGCCAGACCT GTGGAGACAC AGTGTGTGCC TTTGGGGCTG TGTGCTCAGC GGGGCAGTGC 
GTGTGTCCCC GGTGTGAGCG CCCTCCACCC GGCCCCGTGT GTGGCAGCGA TGGCGTGACC 
TACGGCAGTT CATGCGAGCT CCGTGAGGCT GCCTGTCAGC AGCAAACACA GATCGAGGAG 
GCCTGGGCGG GGCCGTGTGA GCAAGCTGAG TGTGGCTCCG GAGGCTCGGG CTCGGGAGAG 
GACGGCGAGT GTGAGCCTGA GCTGTGCCGG CAGCGAGGTG GCATCTGGGA TGAGGACTCG 
GAGGACGGGC CATGCGTCTG CGGCTTCAGC TGCCAGGGCG TCCTGAGGAG CCCGGTGTGT 
GGCTCGGATG GAGTCACCTA CCGCACCGAA TGTGAGCTGA AGAAGGCCAG GTGTGAATCA 
CAGCCAGAGC TGTATGTCGT GGCCCAGGGA GCCTGCCGTG GCCCCACTTT GGCCCCGTTG 
CCACCTGCGA TTCCCCTGCA CTGTGCTCAG ACCCCCTACG GTTGCTGCCA GGACAACATC 
ACTGTTGCCC AGGGTGTTGG CCTGGCTGGC TGCCCCAGCT CATGCCAGTG TAACCCACAC 
GGCTCCTACG GCGGCACCTG TGACCCTGTC ACAGGTCAGT GCTCCTGCCG CCCAGGTGTG 
GGGGGCCTCA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG TATCGTCACT 
GACGGCCGGA GCGGCTGTAC CCCCTGCAGC TGTGACCCCC GGGGTGCCGT GCGGGACGAC 
TGTGAGCAGA TGACTGGGCT ATGTTCCTGT AAGCCTGGGG TGGCCGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCGTGC CCTGGGCCCT GCTGGCTGTG AAACAGACTC TTCGCCACCC 
AAGACCTGTG CTGAGATGCT TTGTGAGTTT GGGGCATCCT GTGTGGAGGA GGCTGGCTCT 
GCCCACTGCG TGTGCCCAAC ACCCACCTGT CCAGGGGCCG ATGCTACCAA GGTCTGCGGG 
TCAGATGGAG TCACTTATGG CAATGAGTGC CAGCTGAGGA CCATCGCCTG CCGCCAGGGC 
TTGGAGATCT CTATCCAAAG CTTCGGCCCC TGCCAGGAAG GCATCACTTC TGGTCCCCGT 
GTGACATCCG CATCTGTGGC TCCCTCAGCA CTTGACCTGA ACAAGGCACC ATTGCCGCCC 
CCCAGCGCCT TGCCCCTGGC TCCCAGCAGT ACCCGCCACA GCCAGCCCAC TTCTCGAGCC 
TCATCACAAC CTTGGACCAT GGCCAGCATC CCCAGGACCA CTGCAAGGCC AGTGCTGACC 
ATGCCTCCCG TGGCCCCCTC CCCGGCAGCC AGCCTCGTCA CCTCTGCCTT CGGCGAATCT 
GGCAGCGCTG ATGGGAGTGG TGACGAGGAG CTGAGTGGTG ACTTGGAGGC CAGTGGGGCT 
GGCTCAGGAG GACTTGAGTC CCCGGAAAGA GACAGTGCTG GGACCCCTGG GCTACCCATG 
GAAAGGGCTT CCTGCTACAA CTCGCCTATG GGCTGCTGCT CAGATGGCAA GACACCCTCC 
CTGGACGCAG AGGGCTCTAA CTGTCCTGCC ACCAAGGTGT TCCAGGGTGT GCTGGAGCTT 
GAGGGGGTCG AGGGGCAAGA ACTGTTCTAC ACGCCGGAGA TGGCAGACCC CAAGTCTGAG 
CTGTTTGGGG AGACTGCCAG GAGCATTGAG AGCGCACTGG ATGACCTCTT CCGGAACTCG 
GACGTTAAGA AGGACTTCCG AAGTGTCCGC CTACGGGACT TGGGGCCAGG CAGCTCGGTC 
CGGGCCATTG TGGAAGTGCA CTTTGATCCC ACCACAGCCT TCAGGGCGTC TGACGTGGGC 
CGGGCCCTGC TCCGGCAGCT CCAGGCGTCC AGGCGCAGGT CCCTGGGCGT GAGGCGGCCT 
CTGCAAGAAC ATGTGCGGTT CATGGACTTC GACTGGTTTC CTGCATTCTT CACGGGAGCC 
ACCCCAGCTG CAGCCACGGC CAGAGCCACC ACGGTGGCCC GCCTGCCACC TTCAGCTGCA 
ACCCCTCGGG CCCACTTTCC CAGTCACACA AGCCGGCCCG TCAGCAGGAC CACACCTCCC 
GCGACCACGC GCCAGCCCCC CACCACTGCC CCTAGTCGTG TGCCTGGACG CCGTCCCCTG 
CCCCCAGGCA CCCAGCAGCC CCGAAGGCCC TGTGATTCCC AGCCCTGCCT CCATGGGGGG 
ACCTGCCAGG ACCAGGGCTC GGGTGCAGAC TTCACCTGCA GCTGCCCAGC AGGCACGGGG 
GGTGCCGTCT GTGAGAAGGC CCTGCACCCC TCCGTGCCAG CCTTTGGGGG CCACTCCTTC 
CTGGCCTTTC CCACCCTCCG TGCCTACCAC ACCCTGCGCC TGGCGCTCGA GTTCCGGGCG 
CTGGAGCCCC AGGGGCTGCT GCTCTACAAC GGCAATGCCC GGGGCAAGGA CTTCCTGGGA 
CTGGTGCTGC TGGGAGGCCG TGTGCAGTTC AGGTTCGACA CAGGCTCAGG GCCCGCGGTG 
CTGACCAGCT CCGTGCCCGT GCAGCCCGGC CGCTGGCATC ACCTGGAGCT GTCTCGGCAC 
TGGCGCCAAG GCACTCTCTC CGTGGACGGT GAGACTCCTG TTCTGGGCCA GAGCCCCAGT 
GGCACTGACG GTCTCAACCT GGACACTGAT CTTTTCGTGG GTGGCGTCCC TGAGGATCAG 
GCTTCCACGG TGCTAGAGCG GACCTCTGTC AGCATCGGTC TTAGGGGTTG TATCCGCTTG 
CTGGATGTCA ACAATCAGCG GCTGGAGCTG AGCAGCTGGC CAGAGTCTGC GACTCGAAGC 
TCCGGAGTGG GCAAGTGCGG GGACCACCCC TGTCTGCCTA GCCCCTGCCT CGGAGGGGCC 
CCGTGCCAGG CCCTGGAGGC AGGCAGGTTC CACTGTCAGT GCCCACCTGG CCGCTTTGGC 
CCAACCTGTG CCGACGAGAA GGACCCTTGT CAGCCGAACC CCTGCCATGG AGCAGCCCCC 
TGCCGCGTGC TGCCCCAGGG AGAGGCCAAG TGCGAGTGCC CCCATGGCCG GGAGGGCAGC 
CTCTGCCAGA CAGTCTCAGA GCCGGAGGAC AACCAGCCGT TCCTGGCTGA TTTCAGCAGC 
TTCTCCTACC TGGAACTGAA AGGCCTGCAC ACCTTTGAGC GGGACCTGGG GGAGAAGATG 
GCACTGGAGG TGGTGTTCCT GGCTCGGAGC CCCAGCGGCC TGCTCCTCTA TAATGGACAG 
AAGACAGATG GCAAGGGCGA CTTTGTGTCC CTGGCGCTGC ACAATGGCCT CCTGGAGTTC 
CGCTACGATC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA AGGAGCCAGT GGCCCTGGGT 
GCCTGGACCA GGGTCTCCCT GGAGCGAAAT GGACGCAAAG GAGCCATGCG GGTTGGTGAT 
GGGCCTCGCG TGCTGGGGGA GTCCCCGGTG CCACACACCG TTCTCAACCT AAAGGAGCCG 
CTCTTTGTTG GGGGGGCCCC TGACTTCAGC AAGCTGGCCC GAGCAGCTGC TGTGTCCTCT 
GGCTTTGATG GAGCCATCCA GCTGGTCTCC CTGAATGGCC GCCAACTGCT GACCCGTGAG 
AACGTGGTCC GGGCCGTGGA CGTCTCGTCC TTTGCAGACC ACCCTTGTAC CCAGGCTGAG 
GGTCAGCCTT GCCTCCATGG GGCTTCCTGT CTCCCCCGCG AGGCCTCCTA TGAGTGCCTG 
TGTCCTGCAG GCTTCTCAGG GCTGCACTGT GAGAAGGGCT TGATTGAGAA GTCAGCTGGG 
GACCTGGACG CACTGGCCTT TGATGGACGG ACCTACATCG AGTACCTCAA CGCTGTGACC 
GAGAGTGAGA AGGCACTCCA GAGCAACCAC TTTGAGCTGA GCCTGCGCAC TGAGGCCACA 
CAGGGGCTGG TGCTGTGGAG TGGCAAGGCC ACGGAGCGGG CTGACTACAT CGCACTGGCC 
ATCGTGGACG GGCGCCTGCA ACTGGCTTAT GACCTGGGCT CCCAGCCCGT GGTGTTGCGC 
TCCACCGTAC CAGTGAATAC CAACCGCTGG TTGCGGGTCA GGGCACACAG GAAACAGAGG 
GAAGGTTCCC TTCAGGTGGG CAACGAGGCC CCTGTGACTG GCTCCTCCCC ACTGGGTGCC 
ACGCAGCTGG ACACAGATGG AGCCCTGTGG CTGGGTGGCC TGGAGAAGCT GCCCACAGGC 
CAGGCCCTGC CCAAAGCCTA TGGCACAGGC TTCGTGGGCT GCCTGCGGGA CGTGGTCGTG 
GGCCAGCGCC CGGTGCACCT GCTGGAGGAC GCCATCACCA AGCCAGAGCT TCGGCCCTGC 
CCTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AGRN
Entrez Gene ID	525795
Full Name	agrin
General protein information	Preferred Names agrin Names agrin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Canis lupus familiaris (dog)	AGRN	XP_005620416.1
Homo sapiens (human)	AGRN	NP_940978.2
Rattus norvegicus (Norway rat)	Agrn	NP_786930.1
Caenorhabditis elegans (roundworm)	agr-1	NP_001022152.3
Xenopus tropicalis (tropical clawed frog)	agrn	XP_002936430.2
	AGRN	XP_001088755.2
Mus musculus (house mouse)	Agrn	NP_067617.3
Gallus gallus (chicken)	AGRN	NP_990858.1
Pan troglodytes (chimpanzee)	LOC100609326	XP_003319002.1
Bos taurus (cattle)	AGRN	XP_005217262.1
Danio rerio (zebrafish)	hm:gc12	NP_001170923.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AGRN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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