Agrn gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_011250177.2	XP_011248479.1	agrin isoform X6
XM_006538494.3	XP_006538557.1	agrin isoform X5
NM_001369026.1	NP_001355955.1	agrin isoform 2
XM_006538492.2	XP_006538555.1	agrin isoform X3
XM_017319921.1	XP_017175410.1	agrin isoform X8
XM_011250178.1	XP_011248480.1	agrin isoform X9
XM_030253098.1	XP_030108958.1	agrin isoform X10
XM_006538491.1	XP_006538554.1	agrin isoform X1
XM_006538491.2	XP_006538554.1	agrin isoform X1
XM_011250176.2	XP_011248478.1	agrin isoform X2
XM_006538494.4	XP_006538557.1	agrin isoform X5
XM_006538492.1	XP_006538555.1	agrin isoform X3
XM_006538493.1	XP_006538556.1	agrin isoform X4
XM_006538495.2	XP_006538558.1	agrin isoform X7
XM_006538496.3	XP_006538559.1	agrin isoform X8
XM_006538493.2	XP_006538556.1	agrin isoform X4
XM_006538495.3	XP_006538558.1	agrin isoform X6
XM_011250176.1	XP_011248478.1	agrin isoform X2
XM_006538496.4	XP_006538559.1	agrin isoform X7
NM_021604.3	NP_067617.3	agrin isoform 1 precursor
NM_001369027.1	NP_001355956.1	agrin isoform 3 precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84689	NM_001369027.1 Latest version!	Mus musculus agrin (Agrn), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu88397	XM_030253098.1 Latest version!	Mus musculus agrin (Agrn), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu33957	XM_006538495.3 Latest version!	Mus musculus agrin (Agrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu33958	XM_006538496.4 Latest version!	Mus musculus agrin (Agrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu33953	XM_006538491.2 Latest version!	Mus musculus agrin (Agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu33954	XM_006538492.2 Latest version!	Mus musculus agrin (Agrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu33955	XM_006538493.2 Latest version!	Mus musculus agrin (Agrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu33956	XM_006538494.4 Latest version!	Mus musculus agrin (Agrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67084	XM_011250176.2 Latest version!	Mus musculus agrin (Agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu12494	NM_021604.3 Latest version!	Mus musculus agrin (Agrn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OMu67085	NM_001369026.1 Latest version!	Mus musculus agrin (Agrn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu67085	XM_011250177.2 Latest version!	Mus musculus agrin (Agrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu67086	XM_011250178.1 Latest version!	Mus musculus agrin (Agrn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu80818	XM_017319921.1 Latest version!	Mus musculus agrin (Agrn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu33953	XM_006538491.1	Mus musculus agrin (Agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu33956	XM_006538494.3	Mus musculus agrin (Agrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu33954	XM_006538492.1	Mus musculus agrin (Agrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu33955	XM_006538493.1	Mus musculus agrin (Agrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu33957	XM_006538495.2	Mus musculus agrin (Agrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu67084	XM_011250176.1	Mus musculus agrin (Agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu33958	XM_006538496.3	Mus musculus agrin (Agrn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

Agrn ( NM_001369027.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355956.1 Mus musculus agrin (Agrn), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84689
Clone ID Related Accession (Same CDS sequence)	NM_001369027.1
Accession Version	NM_001369027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	agrin isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT998563.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY750102.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849389, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGG TCCCAGAAGA 
TCTAGGCTAG CGTTGTGCCC TGTACCCACA AGTGAGCTTC CAGCACATAT GGAAGAACAG 
CACAAACACC ATATTGGAAG GAGGATTGAG GTATCTGTCC CCTGTTCTGG GAAAGAAGCC 
ATCAGCTATG AGGAGTGGCC TGTGGTGTCA TCCATCATCG ATTCCACCAG TTCCTTCTCG 
CCTGGGTCAA ATCACCACCA CGCTACAGCC ATTCCCCATA GGCTTCATGA GGCATCTGCC 
GTGGCTGCCA ACTCAATGGC CTTGCTGGTG TTTTGTGGGC CTCTTAAGAA GCTGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355956.1
CDS	69..848
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEGPRRSRLALCPVPTSELPAHMEEQHKHHIGRRIEVSVPCSGKEAISYEEWPVVSSIIDSTSSFSPGSNHHHATAIPHRLHEASAVAANSMALLVFCGPLKKLS

Target ORF information:

RefSeq Version	NM_001369027.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369027.1

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGG TCCCAGAAGA 
TCTAGGCTAG CGTTGTGCCC TGTACCCACA AGTGAGCTTC CAGCACATAT GGAAGAACAG 
CACAAACACC ATATTGGAAG GAGGATTGAG GTATCTGTCC CCTGTTCTGG GAAAGAAGCC 
ATCAGCTATG AGGAGTGGCC TGTGGTGTCA TCCATCATCG ATTCCACCAG TTCCTTCTCG 
CCTGGGTCAA ATCACCACCA CGCTACAGCC ATTCCCCATA GGCTTCATGA GGCATCTGCC 
GTGGCTGCCA ACTCAATGGC CTTGCTGGTG TTTTGTGGGC CTCTTAAGAA GCTGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_030253098.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108958.1 Mus musculus agrin (Agrn), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu88397
Clone ID Related Accession (Same CDS sequence)	XM_030253098.1
Accession Version	XM_030253098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCC CACACTGTGC CCAAACCCCC TATGGCTGCT GCCAGGACAA TGTCACTGCT 
GCCCAGGGTG TGGGCTTGGC TGGCTGTCCC AGCACCTGCC ATTGCAACCC ACACGGCTCC 
TATAGCGGCA CTTGTGACCC AGTCACAGGG CAGTGCTCCT GCCGACCAGG TGTAGGAGGC 
CTCAGGTGTG ATCGCTGTGA GCCTGGCTTC TGGAACTTCC GTGGCATTGT CACCGATGGA 
CATAGTGGTT GCACTCCCTG CAGCTGTGAC CCTCGGGGTG CTGTAAGAGA TGACTGTGAG 
CAGATGACTG GATTGTGTTC CTGTAGACCT GGTGTGGCTG GTCCCAAGTG TGGGCAGTGT 
CCAGATGGTC AAGCCCTGGG CCATCTTGGC TGTGAAGCAG ATCCCACAAC ACCAGTGACT 
TGTGTGGAAA TGCACTGTGA GTTTGGCGCC TCCTGCGTAG AGGAGGCTGG TTTTGCCCAG 
TGTGTCTGCC CAACTCTCAC ATGTCCAGAG GCTAACTCTA CCAAGGTCTG TGGATCAGAT 
GGTGTCACAT ACGGCAATGA ATGCCAGCTG AAGACCATTG CCTGCCGCCA GCGTCTGGAC 
ATCTCCATTC AGAGTCTTGG TCCATGCCGG GAGAGTGTTG CTCCTGGGGT TTCCCCTACA 
TCTGCATCTA TGACCACCCC AAGGCATATC CTGAGCAGGA CACTGGCGTC TCCCCACAGC 
AGCCTTCCTC TGTCTCCCAG CACTACTGCC CATGATTGGC CCACCCCATT ACCCACATCA 
CCTCAGACCG TAGTCGGCAC CCCCAGGAGC ACTGCAGCCA CACCCTCTGA TGTGGCCAGT 
CTTGCTACAG CGATCTTCAG GGAATCTGGC AGCACCAACG GCAGTGGCGA TGAGGAGCTC 
AGTGGCGATG AGGAGGCCAG TGGGGGCGGG TCTGGGGGAC TTGAGCCCCC GGTGGGCAGC 
GTTGTGGTGA CCCACGGGCC ACCCATCGAG AGGGCTTCCT GTTACAACTC ACCTTTGGGC 
TGCTGCTCAG ATGGCAAGAC ACCCTCACTG GACTCAGAAG GCTCCAACTG TCCAGCTACC 
AAGGCATTCC AGGGCGTGCT GGAGCTTGAG GGGGTCGAGG GACAGGAACT GTTCTACACA 
CCAGAGATGG CTGACCCCAA GTCAGAGTTG TTTGGGGAGA CTGCAAGGAG CATTGAGAGC 
ACGCTGGACG ACCTGTTCCG GAATTCGGAT GTTAAGAAGG ACTTCTGGAG CATCCGCCTA 
CGGGAACTGG GGCCTGGCAA ATTAGTCCGT GCCATTGTGG ATGTTCACTT TGACCCCACC 
ACAGCCTTCC AGGCACCAGA TGTGGGTCAG GCCTTGCTCC AACAGATCCA GGTATCCAGG 
CCGTGGGCCC TGGCAGTGAG GAGGCCTCTG CGGGAGCATG TGCGATTCTT GGACTTTGAC 
TGGTTTCCCA CTTTTTTTAC GGGAGCTGCA ACAGGAACCA CAGCTGCTGT GGCCACAGCC 
AGAGCCACCA CTGTGAGCCG ACTGTCTGCC TCTTCTGTCA CCCCACGAGT CTACCCCAGT 
TACACCAGCC GGCCTGTTGG CAGAACTACG GCACCGCTAA CCACTCGCCG GCCACCAACC 
ACTACCGCCA GTATTGACCG ACCTCGGACT CCAGGCCCGC AACGGCCCCC AAAGTCCTGT 
GATTCCCAGC CTTGCCTCCA CGGAGGTACC TGCCAGGACC TGGATTCTGG CAAGGGTTTC 
AGCTGCAGCT GTACTGCAGG CAGGGCTGGC ACTGTCTGTG AGAAAGTGCA GCTCCCCTCT 
GTGCCAGCTT TTAAGGGCCA CTCCTTCTTG GCCTTCCCCA CCCTCCGAGC CTACCACACG 
CTGCGTCTGG CACTAGAATT CCGGGCGCTG GAGACAGAGG GACTGCTGCT CTACAATGGC 
AATGCACGTG GCAAAGATTT CCTGGCTCTG GCTCTGTTGG ATGGTCATGT ACAGTTCAGG 
TTCGACACGG GCTCAGGGCC GGCGGTGCTA ACAAGCTTAG TGCCAGTGGA ACCGGGACGG 
TGGCACCGCC TCGAGTTGTC ACGGCATTGG CGGCAGGGCA CACTTTCTGT GGATGGCGAG 
GCTCCTGTTG TAGGTGAAAG TCCGAGTGGC ACTGATGGCC TCAACTTGGA CACGAAGCTC 
TATGTGGGTG GTCTCCCAGA AGAACAAGTT GCCACGGTGC TTGATCGGAC CTCTGTGGGC 
ATCGGCCTGA AAGGATGCAT TCGTATGTTG GACATCAACA ACCAGCAGCT GGAGCTGAGC 
GATTGGCAGA GGGCTGTGGT TCAAAGCTCT GGTGTGGGGG AATGCGGAGA CCATCCCTGC 
TCACCTAACC CCTGCCATGG CGGGGCCCTC TGCCAGGCCC TGGAGGCTGG CGTGTTCCTC 
TGTCAGTGCC CACCTGGCCG CTTTGGCCCA ACTTGTGCAG ATGAAAAGAA CCCCTGCCAA 
CCGAACCCCT GCCACGGGTC AGCCCCCTGC CATGTGCTTT CCAGGGGTGG GGCCAAGTGT 
GCGTGCCCCC TGGGACGCAG TGGTTCCTTC TGTGAGACAG TCCTGGAGAA TGCTGGCTCC 
CGGCCCTTCC TGGCTGACTT TAATGGCTTC TCCTACCTGG AACTGAAAGG CTTGCACACC 
TTCGAGAGAG ACCTAGGGGA GAAGATGGCG CTGGAGATGG TGTTCTTGGC TCGTGGGCCC 
AGTGGCTTAC TCCTCTACAA TGGGCAGAAG ACGGATGGCA AGGGGGACTT TGTATCCCTG 
GCCCTGCATA ACCGGCACCT AGAGTTCCGC TATGACCTTG GCAAGGGGGC TGCAATCATC 
AGGAGCAAAG AGCCCATAGC CCTGGGCACC TGGGTTAGGG TATTCCTGGA ACGAAATGGC 
CGCAAGGGTG CCCTTCAAGT GGGTGATGGG CCCCGTGTGC TAGGGGAATC TCCGAAATCC 
CGCAAGGTCC CGCACACCAT GCTCAACCTC AAGGAGCCCC TCTATGTGGG GGGAGCTCCT 
GACTTCAGCA AGCTGGCTCG GGGCGCTGCA GTGGCCTCCG GCTTTGATGG TGCCATCCAG 
CTGGTGTCTC TAAGAGGCCA TCAGCTGCTG ACTCAGGAGC ATGTGTTGCG GGCAGTAGAT 
GTAGCGCCTT TTGCAGGCCA CCCTTGTACC CAGGCCGTGG ACAACCCCTG CCTTAATGGG 
GGCTCCTGTA TCCCGAGGGA AGCCACTTAT GAGTGCCTGT GTCCTGGGGG CTTCTCTGGG 
CTGCACTGCG AGAAGGGGAT AGTTGAGAAG TCAGTGGGGG ACCTAGAAAC ACTGGCCTTT 
GATGGGCGGA CCTACATCGA GTACCTCAAT GCTGTGACTG AGAGCGAGCT GACCAATGAG 
ATCCCAGCCC CCGAAACTCT GGATTCCCGG GCCCTTTTCA GTGAGAAAGC GCTGCAGAGC 
AACCACTTTG AGCTGAGCTT ACGCACTGAG GCCACGCAGG GGCTGGTGCT GTGGATTGGA 
AAGGTTGGAG AACGTGCAGA CTACATGGCT CTGGCCATTG TGGATGGGCA CCTACAACTG 
AGCTATGACC TAGGCTCCCA GCCAGTTGTG CTGCGCTCCA CTGTGAAGGT CAACACCAAC 
CGCTGGCTTC GAGTCAGGGC TCACAGGGAG CACAGGGAAG GTTCCCTTCA GGTGGGCAAT 
GAAGCCCCTG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AATTGGACAC AGATGGAGCC 
CTGTGGCTTG GAGGCCTACA GAAGCTTCCT GTGGGGCAGG CTCTCCCCAA GGCCTATGGC 
ACGGGTTTTG TGGGCTGTCT GCGGGACGTG GTAGTGGGCC ATCGCCAGCT GCATCTGCTG 
GAGGACGCTG TCACCAAACC AGAGCTAAGA CCCTGCCCCA CTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108958.1
CDS	921..4808
Translation	MASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_030253098.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253098.1

ORF Insert Sequence:

ATGGCCTCCC CACACTGTGC CCAAACCCCC TATGGCTGCT GCCAGGACAA TGTCACTGCT 
GCCCAGGGTG TGGGCTTGGC TGGCTGTCCC AGCACCTGCC ATTGCAACCC ACACGGCTCC 
TATAGCGGCA CTTGTGACCC AGTCACAGGG CAGTGCTCCT GCCGACCAGG TGTAGGAGGC 
CTCAGGTGTG ATCGCTGTGA GCCTGGCTTC TGGAACTTCC GTGGCATTGT CACCGATGGA 
CATAGTGGTT GCACTCCCTG CAGCTGTGAC CCTCGGGGTG CTGTAAGAGA TGACTGTGAG 
CAGATGACTG GATTGTGTTC CTGTAGACCT GGTGTGGCTG GTCCCAAGTG TGGGCAGTGT 
CCAGATGGTC AAGCCCTGGG CCATCTTGGC TGTGAAGCAG ATCCCACAAC ACCAGTGACT 
TGTGTGGAAA TGCACTGTGA GTTTGGCGCC TCCTGCGTAG AGGAGGCTGG TTTTGCCCAG 
TGTGTCTGCC CAACTCTCAC ATGTCCAGAG GCTAACTCTA CCAAGGTCTG TGGATCAGAT 
GGTGTCACAT ACGGCAATGA ATGCCAGCTG AAGACCATTG CCTGCCGCCA GCGTCTGGAC 
ATCTCCATTC AGAGTCTTGG TCCATGCCGG GAGAGTGTTG CTCCTGGGGT TTCCCCTACA 
TCTGCATCTA TGACCACCCC AAGGCATATC CTGAGCAGGA CACTGGCGTC TCCCCACAGC 
AGCCTTCCTC TGTCTCCCAG CACTACTGCC CATGATTGGC CCACCCCATT ACCCACATCA 
CCTCAGACCG TAGTCGGCAC CCCCAGGAGC ACTGCAGCCA CACCCTCTGA TGTGGCCAGT 
CTTGCTACAG CGATCTTCAG GGAATCTGGC AGCACCAACG GCAGTGGCGA TGAGGAGCTC 
AGTGGCGATG AGGAGGCCAG TGGGGGCGGG TCTGGGGGAC TTGAGCCCCC GGTGGGCAGC 
GTTGTGGTGA CCCACGGGCC ACCCATCGAG AGGGCTTCCT GTTACAACTC ACCTTTGGGC 
TGCTGCTCAG ATGGCAAGAC ACCCTCACTG GACTCAGAAG GCTCCAACTG TCCAGCTACC 
AAGGCATTCC AGGGCGTGCT GGAGCTTGAG GGGGTCGAGG GACAGGAACT GTTCTACACA 
CCAGAGATGG CTGACCCCAA GTCAGAGTTG TTTGGGGAGA CTGCAAGGAG CATTGAGAGC 
ACGCTGGACG ACCTGTTCCG GAATTCGGAT GTTAAGAAGG ACTTCTGGAG CATCCGCCTA 
CGGGAACTGG GGCCTGGCAA ATTAGTCCGT GCCATTGTGG ATGTTCACTT TGACCCCACC 
ACAGCCTTCC AGGCACCAGA TGTGGGTCAG GCCTTGCTCC AACAGATCCA GGTATCCAGG 
CCGTGGGCCC TGGCAGTGAG GAGGCCTCTG CGGGAGCATG TGCGATTCTT GGACTTTGAC 
TGGTTTCCCA CTTTTTTTAC GGGAGCTGCA ACAGGAACCA CAGCTGCTGT GGCCACAGCC 
AGAGCCACCA CTGTGAGCCG ACTGTCTGCC TCTTCTGTCA CCCCACGAGT CTACCCCAGT 
TACACCAGCC GGCCTGTTGG CAGAACTACG GCACCGCTAA CCACTCGCCG GCCACCAACC 
ACTACCGCCA GTATTGACCG ACCTCGGACT CCAGGCCCGC AACGGCCCCC AAAGTCCTGT 
GATTCCCAGC CTTGCCTCCA CGGAGGTACC TGCCAGGACC TGGATTCTGG CAAGGGTTTC 
AGCTGCAGCT GTACTGCAGG CAGGGCTGGC ACTGTCTGTG AGAAAGTGCA GCTCCCCTCT 
GTGCCAGCTT TTAAGGGCCA CTCCTTCTTG GCCTTCCCCA CCCTCCGAGC CTACCACACG 
CTGCGTCTGG CACTAGAATT CCGGGCGCTG GAGACAGAGG GACTGCTGCT CTACAATGGC 
AATGCACGTG GCAAAGATTT CCTGGCTCTG GCTCTGTTGG ATGGTCATGT ACAGTTCAGG 
TTCGACACGG GCTCAGGGCC GGCGGTGCTA ACAAGCTTAG TGCCAGTGGA ACCGGGACGG 
TGGCACCGCC TCGAGTTGTC ACGGCATTGG CGGCAGGGCA CACTTTCTGT GGATGGCGAG 
GCTCCTGTTG TAGGTGAAAG TCCGAGTGGC ACTGATGGCC TCAACTTGGA CACGAAGCTC 
TATGTGGGTG GTCTCCCAGA AGAACAAGTT GCCACGGTGC TTGATCGGAC CTCTGTGGGC 
ATCGGCCTGA AAGGATGCAT TCGTATGTTG GACATCAACA ACCAGCAGCT GGAGCTGAGC 
GATTGGCAGA GGGCTGTGGT TCAAAGCTCT GGTGTGGGGG AATGCGGAGA CCATCCCTGC 
TCACCTAACC CCTGCCATGG CGGGGCCCTC TGCCAGGCCC TGGAGGCTGG CGTGTTCCTC 
TGTCAGTGCC CACCTGGCCG CTTTGGCCCA ACTTGTGCAG ATGAAAAGAA CCCCTGCCAA 
CCGAACCCCT GCCACGGGTC AGCCCCCTGC CATGTGCTTT CCAGGGGTGG GGCCAAGTGT 
GCGTGCCCCC TGGGACGCAG TGGTTCCTTC TGTGAGACAG TCCTGGAGAA TGCTGGCTCC 
CGGCCCTTCC TGGCTGACTT TAATGGCTTC TCCTACCTGG AACTGAAAGG CTTGCACACC 
TTCGAGAGAG ACCTAGGGGA GAAGATGGCG CTGGAGATGG TGTTCTTGGC TCGTGGGCCC 
AGTGGCTTAC TCCTCTACAA TGGGCAGAAG ACGGATGGCA AGGGGGACTT TGTATCCCTG 
GCCCTGCATA ACCGGCACCT AGAGTTCCGC TATGACCTTG GCAAGGGGGC TGCAATCATC 
AGGAGCAAAG AGCCCATAGC CCTGGGCACC TGGGTTAGGG TATTCCTGGA ACGAAATGGC 
CGCAAGGGTG CCCTTCAAGT GGGTGATGGG CCCCGTGTGC TAGGGGAATC TCCGAAATCC 
CGCAAGGTCC CGCACACCAT GCTCAACCTC AAGGAGCCCC TCTATGTGGG GGGAGCTCCT 
GACTTCAGCA AGCTGGCTCG GGGCGCTGCA GTGGCCTCCG GCTTTGATGG TGCCATCCAG 
CTGGTGTCTC TAAGAGGCCA TCAGCTGCTG ACTCAGGAGC ATGTGTTGCG GGCAGTAGAT 
GTAGCGCCTT TTGCAGGCCA CCCTTGTACC CAGGCCGTGG ACAACCCCTG CCTTAATGGG 
GGCTCCTGTA TCCCGAGGGA AGCCACTTAT GAGTGCCTGT GTCCTGGGGG CTTCTCTGGG 
CTGCACTGCG AGAAGGGGAT AGTTGAGAAG TCAGTGGGGG ACCTAGAAAC ACTGGCCTTT 
GATGGGCGGA CCTACATCGA GTACCTCAAT GCTGTGACTG AGAGCGAGCT GACCAATGAG 
ATCCCAGCCC CCGAAACTCT GGATTCCCGG GCCCTTTTCA GTGAGAAAGC GCTGCAGAGC 
AACCACTTTG AGCTGAGCTT ACGCACTGAG GCCACGCAGG GGCTGGTGCT GTGGATTGGA 
AAGGTTGGAG AACGTGCAGA CTACATGGCT CTGGCCATTG TGGATGGGCA CCTACAACTG 
AGCTATGACC TAGGCTCCCA GCCAGTTGTG CTGCGCTCCA CTGTGAAGGT CAACACCAAC 
CGCTGGCTTC GAGTCAGGGC TCACAGGGAG CACAGGGAAG GTTCCCTTCA GGTGGGCAAT 
GAAGCCCCTG TGACTGGCTC TTCCCCGCTG GGTGCCACAC AATTGGACAC AGATGGAGCC 
CTGTGGCTTG GAGGCCTACA GAAGCTTCCT GTGGGGCAGG CTCTCCCCAA GGCCTATGGC 
ACGGGTTTTG TGGGCTGTCT GCGGGACGTG GTAGTGGGCC ATCGCCAGCT GCATCTGCTG 
GAGGACGCTG TCACCAAACC AGAGCTAAGA CCCTGCCCCA CTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538495.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538558.1 Mus musculus agrin (Agrn), transcript variant X6, mRNA.

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CloneID	OMu33957
Clone ID Related Accession (Same CDS sequence)	XM_006538495.2 , XM_006538495.3
Accession Version	XM_006538495.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGCAGC CTGGTGCCTC CGTGCTGGTT 
CGGTACTTCA TGATCCCCTG CAACATCTGC TTGATCCTCT TGGCTACTTC TACGTTGGGC 
TTTGCGGTGC TGCTTTTCCT CAGCAACTAC AAACCTGGGA TCCACTTCAC AGCAGCGCCT 
TCTATGCCTC CTGATGTGTG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG GGCCTCCTGT GTGTGCAAGA AGAATGTCTG CCCTGCTATG 
GTAGCTCCTG TGTGTGGCTC AGATGCTTCC ACCTATAGCA ACGAGTGTGA GCTACAGCGT 
GCACAGTGCA ACCAGCAACG GCGCATCCGC CTACTCCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAATGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC AGCCGATGGA 
CAGACCGCCT CGTGTCTGTG TCCTACAACC TGCTTTGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGATG GTGTTGACTA CCCTAGTGAG TGCCAGCTGC TCCGTCATGC CTGTGCCAAC 
CAGGAGCACA TCTTTAAGAA GTTCGATGGT CCTTGTGACC CCTGCCAGGG CAGCATGTCA 
GACCTGAATC ATATTTGCCG GGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCCGCCCA ACACACACCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA GCCCGTGGCC TGCTTCTCCA GAAAGTGCGC 
TCTGGTCAAT GCCAGACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCTGTATGC 
CTGTCCCGCC GCGGCCGTCC CCACTGTTCC TGCGATCGCG TCACCTGTGA TGGGGCTTAC 
AGGCCAGTGT GTGCCCAGGA TGGGCACACG TATGACAATG ACTGTTGGCG CCAACAGGCC 
GAGTGTCGAC AACAGCAGAC CATTCCCCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCA 
TCCCCGTGCC GTGGAGCGCA GTGTGCATTT GGGGCAACAT GCACAGTGAA GAATGGGAAA 
GCTGTGTGCG AGTGCCAGCG GGTGTGCTCG GGCGGCTACG ATCCTGTGTG CGGCAGTGAT 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCGAGTGG CCCGCAGAGG ACCGTGTGAC CGATGTGGGC AGTGCCGGTT TGGATCCTTG 
TGCGAGGTGG AGACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGTGGCT CTGACGGACA CACATATGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACACACCAGA TCAGCCTATA CGTGGCCTCA GCCGGACACT GCCAGACCTG TGGAGAAACA 
GTTTGTACCT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCCCCACCTG GCCCTGTGTG CGGCAGTGAT GGCGTCACCT ACCTCAGTGC CTGTGAGCTC 
CGAGAGGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCGTGCAGG GCCGTGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGG TCTGGGGAAG ACAATGCGTG TGAGCAGGAG 
CTGTGTCGGC AGCATGGTGG TGTCTGGGAT GAGGACTCAG AAGACGGGCC GTGTGTCTGT 
GACTTTAGTT GCCAGAGTGT CCTTAAAAGC CCAGTGTGTG GCTCAGATGG AGTCACCTAT 
AGCACGGAGT GCCATCTGAA GAAGGCCAGA TGTGAAGCGC GGCAAGAGCT GTACGTCGCT 
GCTCAGGGAG CCTGCCGGGG CCCTACCTTG GCTCCACTGC TACCTATGGC CTCCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATGTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCACACG GCTCCTATAG CGGCACTTGT 
GACCCAGTCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GTGACCCTCG GGGTGCTGTA AGAGATGACT GTGAGCAGAT GACTGGATTG 
TGTTCCTGTA GACCTGGTGT GGCTGGTCCC AAGTGTGGGC AGTGTCCAGA TGGTCAAGCC 
CTGGGCCATC TTGGCTGTGA AGCAGATCCC ACAACACCAG TGACTTGTGT GGAAATGCAC 
TGTGAGTTTG GCGCCTCCTG CGTAGAGGAG GCTGGTTTTG CCCAGTGTGT CTGCCCAACT 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGTGGAT CAGATGGTGT CACATACGGC 
AATGAATGCC AGCTGAAGAC CATTGCCTGC CGCCAGCGTC TGGACATCTC CATTCAGAGT 
CTTGGTCCAT GCCGGGAGAG TGTTGCTCCT GGGGTTTCCC CTACATCTGC ATCTATGACC 
ACCCCAAGGC ATATCCTGAG CAGGACACTG GCGTCTCCCC ACAGCAGCCT TCCTCTGTCT 
CCCAGCACTA CTGCCCATGA TTGGCCCACC CCATTACCCA CATCACCTCA GACCGTAGTC 
GGCACCCCCA GGAGCACTGC AGCCACACCC TCTGATGTGG CCAGTCTTGC TACAGCGATC 
TTCAGGGAAT CTGGCAGCAC CAACGGCAGT GGCGATGAGG AGCTCAGTGG CGATGAGGAG 
GCCAGTGGGG GCGGGTCTGG GGGACTTGAG CCCCCGGTGG GCAGCGTTGT GGTGACCCAC 
GGGCCACCCA TCGAGAGGGC TTCCTGTTAC AACTCACCTT TGGGCTGCTG CTCAGATGGC 
AAGACACCCT CACTGGACTC AGAAGGCTCC AACTGTCCAG CTACCAAGGC ATTCCAGGGC 
GTGCTGGAGC TTGAGGGGGT CGAGGGACAG GAACTGTTCT ACACACCAGA GATGGCTGAC 
CCCAAGTCAG AGTTGTTTGG GGAGACTGCA AGGAGCATTG AGAGCACGCT GGACGACCTG 
TTCCGGAATT CGGATGTTAA GAAGGACTTC TGGAGCATCC GCCTACGGGA ACTGGGGCCT 
GGCAAATTAG TCCGTGCCAT TGTGGATGTT CACTTTGACC CCACCACAGC CTTCCAGGCA 
CCAGATGTGG GTCAGGCCTT GCTCCAACAG ATCCAGGTAT CCAGGCCGTG GGCCCTGGCA 
GTGAGGAGGC CTCTGCGGGA GCATGTGCGA TTCTTGGACT TTGACTGGTT TCCCACTTTT 
TTTACGGGAG CTGCAACAGG AACCACAGCT GCTGTGGCCA CAGCCAGAGC CACCACTGTG 
AGCCGACTGT CTGCCTCTTC TGTCACCCCA CGAGTCTACC CCAGTTACAC CAGCCGGCCT 
GTTGGCAGAA CTACGGCACC GCTAACCACT CGCCGGCCAC CAACCACTAC CGCCAGTATT 
GACCGACCTC GGACTCCAGG CCCGCAACGG CCCCCAAAGT CCTGTGATTC CCAGCCTTGC 
CTCCACGGAG GTACCTGCCA GGACCTGGAT TCTGGCAAGG GTTTCAGCTG CAGCTGTACT 
GCAGGCAGGG CTGGCACTGT CTGTGAGAAA GTGCAGCTCC CCTCTGTGCC AGCTTTTAAG 
GGCCACTCCT TCTTGGCCTT CCCCACCCTC CGAGCCTACC ACACGCTGCG TCTGGCACTA 
GAATTCCGGG CGCTGGAGAC AGAGGGACTG CTGCTCTACA ATGGCAATGC ACGTGGCAAA 
GATTTCCTGG CTCTGGCTCT GTTGGATGGT CATGTACAGT TCAGGTTCGA CACGGGCTCA 
GGGCCGGCGG TGCTAACAAG CTTAGTGCCA GTGGAACCGG GACGGTGGCA CCGCCTCGAG 
TTGTCACGGC ATTGGCGGCA GGGCACACTT TCTGTGGATG GCGAGGCTCC TGTTGTAGGT 
GAAAGTCCGA GTGGCACTGA TGGCCTCAAC TTGGACACGA AGCTCTATGT GGGTGGTCTC 
CCAGAAGAAC AAGTTGCCAC GGTGCTTGAT CGGACCTCTG TGGGCATCGG CCTGAAAGGA 
TGCATTCGTA TGTTGGACAT CAACAACCAG CAGCTGGAGC TGAGCGATTG GCAGAGGGCT 
GTGGTTCAAA GCTCTGGTGT GGGGGAATGC GGAGACCATC CCTGCTCACC TAACCCCTGC 
CATGGCGGGG CCCTCTGCCA GGCCCTGGAG GCTGGCGTGT TCCTCTGTCA GTGCCCACCT 
GGCCGCTTTG GCCCAACTTG TGCAGATGAA AAGAACCCCT GCCAACCGAA CCCCTGCCAC 
GGGTCAGCCC CCTGCCATGT GCTTTCCAGG GGTGGGGCCA AGTGTGCGTG CCCCCTGGGA 
CGCAGTGGTT CCTTCTGTGA GACAGTCCTG GAGAATGCTG GCTCCCGGCC CTTCCTGGCT 
GACTTTAATG GCTTCTCCTA CCTGGAACTG AAAGGCTTGC ACACCTTCGA GAGAGACCTA 
GGGGAGAAGA TGGCGCTGGA GATGGTGTTC TTGGCTCGTG GGCCCAGTGG CTTACTCCTC 
TACAATGGGC AGAAGACGGA TGGCAAGGGG GACTTTGTAT CCCTGGCCCT GCATAACCGG 
CACCTAGAGT TCCGCTATGA CCTTGGCAAG GGGGCTGCAA TCATCAGGAG CAAAGAGCCC 
ATAGCCCTGG GCACCTGGGT TAGGGTATTC CTGGAACGAA ATGGCCGCAA GGGTGCCCTT 
CAAGTGGGTG ATGGGCCCCG TGTGCTAGGG GAATCTCCGA AATCCCGCAA GGTCCCGCAC 
ACCATGCTCA ACCTCAAGGA GCCCCTCTAT GTGGGGGGAG CTCCTGACTT CAGCAAGCTG 
GCTCGGGGCG CTGCAGTGGC CTCCGGCTTT GATGGTGCCA TCCAGCTGGT GTCTCTAAGA 
GGCCATCAGC TGCTGACTCA GGAGCATGTG TTGCGGGCAG TAGATGTAGC GCCTTTTGCA 
GGCCACCCTT GTACCCAGGC CGTGGACAAC CCCTGCCTTA ATGGGGGCTC CTGTATCCCG 
AGGGAAGCCA CTTATGAGTG CCTGTGTCCT GGGGGCTTCT CTGGGCTGCA CTGCGAGAAG 
GGGATAGTTG AGAAGTCAGT GGGGGACCTA GAAACACTGG CCTTTGATGG GCGGACCTAC 
ATCGAGTACC TCAATGCTGT GACTGAGAGT GAGAAAGCGC TGCAGAGCAA CCACTTTGAG 
CTGAGCTTAC GCACTGAGGC CACGCAGGGG CTGGTGCTGT GGATTGGAAA GGTTGGAGAA 
CGTGCAGACT ACATGGCTCT GGCCATTGTG GATGGGCACC TACAACTGAG CTATGACCTA 
GGCTCCCAGC CAGTTGTGCT GCGCTCCACT GTGAAGGTCA ACACCAACCG CTGGCTTCGA 
GTCAGGGCTC ACAGGGAGCA CAGGGAAGGT TCCCTTCAGG TGGGCAATGA AGCCCCTGTG 
ACTGGCTCTT CCCCGCTGGG TGCCACACAA TTGGACACAG ATGGAGCCCT GTGGCTTGGA 
GGCCTACAGA AGCTTCCTGT GGGGCAGGCT CTCCCCAAGG CCTATGGCAC GGGTTTTGTG 
GGCTGTCTGC GGGACGTGGT AGTGGGCCAT CGCCAGCTGC ATCTGCTGGA GGACGCTGTC 
ACCAAACCAG AGCTAAGACC CTGCCCCACT CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538558.1
CDS	3029..8824
Translation	MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538495.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538495.3

ORF Insert Sequence:

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGCAGC CTGGTGCCTC CGTGCTGGTT 
CGGTACTTCA TGATCCCCTG CAACATCTGC TTGATCCTCT TGGCTACTTC TACGTTGGGC 
TTTGCGGTGC TGCTTTTCCT CAGCAACTAC AAACCTGGGA TCCACTTCAC AGCAGCGCCT 
TCTATGCCTC CTGATGTGTG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG GGCCTCCTGT GTGTGCAAGA AGAATGTCTG CCCTGCTATG 
GTAGCTCCTG TGTGTGGCTC AGATGCTTCC ACCTATAGCA ACGAGTGTGA GCTACAGCGT 
GCACAGTGCA ACCAGCAACG GCGCATCCGC CTACTCCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAATGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC AGCCGATGGA 
CAGACCGCCT CGTGTCTGTG TCCTACAACC TGCTTTGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGATG GTGTTGACTA CCCTAGTGAG TGCCAGCTGC TCCGTCATGC CTGTGCCAAC 
CAGGAGCACA TCTTTAAGAA GTTCGATGGT CCTTGTGACC CCTGCCAGGG CAGCATGTCA 
GACCTGAATC ATATTTGCCG GGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCCGCCCA ACACACACCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA GCCCGTGGCC TGCTTCTCCA GAAAGTGCGC 
TCTGGTCAAT GCCAGACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCTGTATGC 
CTGTCCCGCC GCGGCCGTCC CCACTGTTCC TGCGATCGCG TCACCTGTGA TGGGGCTTAC 
AGGCCAGTGT GTGCCCAGGA TGGGCACACG TATGACAATG ACTGTTGGCG CCAACAGGCC 
GAGTGTCGAC AACAGCAGAC CATTCCCCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCA 
TCCCCGTGCC GTGGAGCGCA GTGTGCATTT GGGGCAACAT GCACAGTGAA GAATGGGAAA 
GCTGTGTGCG AGTGCCAGCG GGTGTGCTCG GGCGGCTACG ATCCTGTGTG CGGCAGTGAT 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCGAGTGG CCCGCAGAGG ACCGTGTGAC CGATGTGGGC AGTGCCGGTT TGGATCCTTG 
TGCGAGGTGG AGACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGTGGCT CTGACGGACA CACATATGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACACACCAGA TCAGCCTATA CGTGGCCTCA GCCGGACACT GCCAGACCTG TGGAGAAACA 
GTTTGTACCT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCCCCACCTG GCCCTGTGTG CGGCAGTGAT GGCGTCACCT ACCTCAGTGC CTGTGAGCTC 
CGAGAGGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCGTGCAGG GCCGTGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGG TCTGGGGAAG ACAATGCGTG TGAGCAGGAG 
CTGTGTCGGC AGCATGGTGG TGTCTGGGAT GAGGACTCAG AAGACGGGCC GTGTGTCTGT 
GACTTTAGTT GCCAGAGTGT CCTTAAAAGC CCAGTGTGTG GCTCAGATGG AGTCACCTAT 
AGCACGGAGT GCCATCTGAA GAAGGCCAGA TGTGAAGCGC GGCAAGAGCT GTACGTCGCT 
GCTCAGGGAG CCTGCCGGGG CCCTACCTTG GCTCCACTGC TACCTATGGC CTCCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATGTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCACACG GCTCCTATAG CGGCACTTGT 
GACCCAGTCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GTGACCCTCG GGGTGCTGTA AGAGATGACT GTGAGCAGAT GACTGGATTG 
TGTTCCTGTA GACCTGGTGT GGCTGGTCCC AAGTGTGGGC AGTGTCCAGA TGGTCAAGCC 
CTGGGCCATC TTGGCTGTGA AGCAGATCCC ACAACACCAG TGACTTGTGT GGAAATGCAC 
TGTGAGTTTG GCGCCTCCTG CGTAGAGGAG GCTGGTTTTG CCCAGTGTGT CTGCCCAACT 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGTGGAT CAGATGGTGT CACATACGGC 
AATGAATGCC AGCTGAAGAC CATTGCCTGC CGCCAGCGTC TGGACATCTC CATTCAGAGT 
CTTGGTCCAT GCCGGGAGAG TGTTGCTCCT GGGGTTTCCC CTACATCTGC ATCTATGACC 
ACCCCAAGGC ATATCCTGAG CAGGACACTG GCGTCTCCCC ACAGCAGCCT TCCTCTGTCT 
CCCAGCACTA CTGCCCATGA TTGGCCCACC CCATTACCCA CATCACCTCA GACCGTAGTC 
GGCACCCCCA GGAGCACTGC AGCCACACCC TCTGATGTGG CCAGTCTTGC TACAGCGATC 
TTCAGGGAAT CTGGCAGCAC CAACGGCAGT GGCGATGAGG AGCTCAGTGG CGATGAGGAG 
GCCAGTGGGG GCGGGTCTGG GGGACTTGAG CCCCCGGTGG GCAGCGTTGT GGTGACCCAC 
GGGCCACCCA TCGAGAGGGC TTCCTGTTAC AACTCACCTT TGGGCTGCTG CTCAGATGGC 
AAGACACCCT CACTGGACTC AGAAGGCTCC AACTGTCCAG CTACCAAGGC ATTCCAGGGC 
GTGCTGGAGC TTGAGGGGGT CGAGGGACAG GAACTGTTCT ACACACCAGA GATGGCTGAC 
CCCAAGTCAG AGTTGTTTGG GGAGACTGCA AGGAGCATTG AGAGCACGCT GGACGACCTG 
TTCCGGAATT CGGATGTTAA GAAGGACTTC TGGAGCATCC GCCTACGGGA ACTGGGGCCT 
GGCAAATTAG TCCGTGCCAT TGTGGATGTT CACTTTGACC CCACCACAGC CTTCCAGGCA 
CCAGATGTGG GTCAGGCCTT GCTCCAACAG ATCCAGGTAT CCAGGCCGTG GGCCCTGGCA 
GTGAGGAGGC CTCTGCGGGA GCATGTGCGA TTCTTGGACT TTGACTGGTT TCCCACTTTT 
TTTACGGGAG CTGCAACAGG AACCACAGCT GCTGTGGCCA CAGCCAGAGC CACCACTGTG 
AGCCGACTGT CTGCCTCTTC TGTCACCCCA CGAGTCTACC CCAGTTACAC CAGCCGGCCT 
GTTGGCAGAA CTACGGCACC GCTAACCACT CGCCGGCCAC CAACCACTAC CGCCAGTATT 
GACCGACCTC GGACTCCAGG CCCGCAACGG CCCCCAAAGT CCTGTGATTC CCAGCCTTGC 
CTCCACGGAG GTACCTGCCA GGACCTGGAT TCTGGCAAGG GTTTCAGCTG CAGCTGTACT 
GCAGGCAGGG CTGGCACTGT CTGTGAGAAA GTGCAGCTCC CCTCTGTGCC AGCTTTTAAG 
GGCCACTCCT TCTTGGCCTT CCCCACCCTC CGAGCCTACC ACACGCTGCG TCTGGCACTA 
GAATTCCGGG CGCTGGAGAC AGAGGGACTG CTGCTCTACA ATGGCAATGC ACGTGGCAAA 
GATTTCCTGG CTCTGGCTCT GTTGGATGGT CATGTACAGT TCAGGTTCGA CACGGGCTCA 
GGGCCGGCGG TGCTAACAAG CTTAGTGCCA GTGGAACCGG GACGGTGGCA CCGCCTCGAG 
TTGTCACGGC ATTGGCGGCA GGGCACACTT TCTGTGGATG GCGAGGCTCC TGTTGTAGGT 
GAAAGTCCGA GTGGCACTGA TGGCCTCAAC TTGGACACGA AGCTCTATGT GGGTGGTCTC 
CCAGAAGAAC AAGTTGCCAC GGTGCTTGAT CGGACCTCTG TGGGCATCGG CCTGAAAGGA 
TGCATTCGTA TGTTGGACAT CAACAACCAG CAGCTGGAGC TGAGCGATTG GCAGAGGGCT 
GTGGTTCAAA GCTCTGGTGT GGGGGAATGC GGAGACCATC CCTGCTCACC TAACCCCTGC 
CATGGCGGGG CCCTCTGCCA GGCCCTGGAG GCTGGCGTGT TCCTCTGTCA GTGCCCACCT 
GGCCGCTTTG GCCCAACTTG TGCAGATGAA AAGAACCCCT GCCAACCGAA CCCCTGCCAC 
GGGTCAGCCC CCTGCCATGT GCTTTCCAGG GGTGGGGCCA AGTGTGCGTG CCCCCTGGGA 
CGCAGTGGTT CCTTCTGTGA GACAGTCCTG GAGAATGCTG GCTCCCGGCC CTTCCTGGCT 
GACTTTAATG GCTTCTCCTA CCTGGAACTG AAAGGCTTGC ACACCTTCGA GAGAGACCTA 
GGGGAGAAGA TGGCGCTGGA GATGGTGTTC TTGGCTCGTG GGCCCAGTGG CTTACTCCTC 
TACAATGGGC AGAAGACGGA TGGCAAGGGG GACTTTGTAT CCCTGGCCCT GCATAACCGG 
CACCTAGAGT TCCGCTATGA CCTTGGCAAG GGGGCTGCAA TCATCAGGAG CAAAGAGCCC 
ATAGCCCTGG GCACCTGGGT TAGGGTATTC CTGGAACGAA ATGGCCGCAA GGGTGCCCTT 
CAAGTGGGTG ATGGGCCCCG TGTGCTAGGG GAATCTCCGA AATCCCGCAA GGTCCCGCAC 
ACCATGCTCA ACCTCAAGGA GCCCCTCTAT GTGGGGGGAG CTCCTGACTT CAGCAAGCTG 
GCTCGGGGCG CTGCAGTGGC CTCCGGCTTT GATGGTGCCA TCCAGCTGGT GTCTCTAAGA 
GGCCATCAGC TGCTGACTCA GGAGCATGTG TTGCGGGCAG TAGATGTAGC GCCTTTTGCA 
GGCCACCCTT GTACCCAGGC CGTGGACAAC CCCTGCCTTA ATGGGGGCTC CTGTATCCCG 
AGGGAAGCCA CTTATGAGTG CCTGTGTCCT GGGGGCTTCT CTGGGCTGCA CTGCGAGAAG 
GGGATAGTTG AGAAGTCAGT GGGGGACCTA GAAACACTGG CCTTTGATGG GCGGACCTAC 
ATCGAGTACC TCAATGCTGT GACTGAGAGT GAGAAAGCGC TGCAGAGCAA CCACTTTGAG 
CTGAGCTTAC GCACTGAGGC CACGCAGGGG CTGGTGCTGT GGATTGGAAA GGTTGGAGAA 
CGTGCAGACT ACATGGCTCT GGCCATTGTG GATGGGCACC TACAACTGAG CTATGACCTA 
GGCTCCCAGC CAGTTGTGCT GCGCTCCACT GTGAAGGTCA ACACCAACCG CTGGCTTCGA 
GTCAGGGCTC ACAGGGAGCA CAGGGAAGGT TCCCTTCAGG TGGGCAATGA AGCCCCTGTG 
ACTGGCTCTT CCCCGCTGGG TGCCACACAA TTGGACACAG ATGGAGCCCT GTGGCTTGGA 
GGCCTACAGA AGCTTCCTGT GGGGCAGGCT CTCCCCAAGG CCTATGGCAC GGGTTTTGTG 
GGCTGTCTGC GGGACGTGGT AGTGGGCCAT CGCCAGCTGC ATCTGCTGGA GGACGCTGTC 
ACCAAACCAG AGCTAAGACC CTGCCCCACT CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538496.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538559.1 Mus musculus agrin (Agrn), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu33958
Clone ID Related Accession (Same CDS sequence)	XM_006538496.3 , XM_006538496.4
Accession Version	XM_006538496.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538496.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCTG ATGTGTGCAG GGGAATGTTA TGTGGCTTTG GTGCTGTGTG TGAACCTAGT 
GTTGAGGATC CAGGCCGGGC CTCCTGTGTG TGCAAGAAGA ATGTCTGCCC TGCTATGGTA 
GCTCCTGTGT GTGGCTCAGA TGCTTCCACC TATAGCAACG AGTGTGAGCT ACAGCGTGCA 
CAGTGCAACC AGCAACGGCG CATCCGCCTA CTCCGCCAAG GGCCATGTGG GTCCCGGGAC 
CCCTGTGCCA ATGTGACCTG CAGTTTCGGT AGTACCTGTG TACCTTCAGC CGATGGACAG 
ACCGCCTCGT GTCTGTGTCC TACAACCTGC TTTGGGGCCC CTGATGGCAC AGTGTGTGGC 
AGTGATGGTG TTGACTACCC TAGTGAGTGC CAGCTGCTCC GTCATGCCTG TGCCAACCAG 
GAGCACATCT TTAAGAAGTT CGATGGTCCT TGTGACCCCT GCCAGGGCAG CATGTCAGAC 
CTGAATCATA TTTGCCGGGT GAACCCACGT ACACGGCACC CAGAAATGCT TCTGCGGCCT 
GAGAACTGCC CCGCCCAACA CACACCTATC TGTGGAGATG ATGGGGTCAC CTATGAAAAC 
GACTGTGTCA TGAGCCGTAT AGGTGCAGCC CGTGGCCTGC TTCTCCAGAA AGTGCGCTCT 
GGTCAATGCC AGACTCGAGA CCAGTGCCCG GAGACCTGCC AGTTTAACTC TGTATGCCTG 
TCCCGCCGCG GCCGTCCCCA CTGTTCCTGC GATCGCGTCA CCTGTGATGG GGCTTACAGG 
CCAGTGTGTG CCCAGGATGG GCACACGTAT GACAATGACT GTTGGCGCCA ACAGGCCGAG 
TGTCGACAAC AGCAGACCAT TCCCCCCAAG CACCAGGGCC CGTGTGACCA GACCCCATCC 
CCGTGCCGTG GAGCGCAGTG TGCATTTGGG GCAACATGCA CAGTGAAGAA TGGGAAAGCT 
GTGTGCGAGT GCCAGCGGGT GTGCTCGGGC GGCTACGATC CTGTGTGCGG CAGTGATGGT 
GTCACTTACG GCAGTGTGTG CGAGCTGGAA TCCATGGCCT GTACCCTTGG GCGGGAAATC 
CGAGTGGCCC GCAGAGGACC GTGTGACCGA TGTGGGCAGT GCCGGTTTGG ATCCTTGTGC 
GAGGTGGAGA CTGGACGCTG TGTGTGCCCC TCTGAGTGTG TGGAGTCAGC CCAGCCCGTA 
TGTGGCTCTG ACGGACACAC ATATGCTAGT GAATGTGAGC TGCATGTCCA CGCCTGTACA 
CACCAGATCA GCCTATACGT GGCCTCAGCC GGACACTGCC AGACCTGTGG AGAAACAGTT 
TGTACCTTTG GGGCTGTGTG CTCAGCTGGA CAGTGTGTAT GTCCCCGTTG TGAGCACCCC 
CCACCTGGCC CTGTGTGCGG CAGTGATGGC GTCACCTACC TCAGTGCCTG TGAGCTCCGA 
GAGGCTGCCT GTCAGCAGCA GGTACAAATT GAGGAGGCCC GTGCAGGGCC GTGTGAGCCG 
GCTGAGTGTG GCTCAGGGGG CTCTGGGTCT GGGGAAGACA ATGCGTGTGA GCAGGAGCTG 
TGTCGGCAGC ATGGTGGTGT CTGGGATGAG GACTCAGAAG ACGGGCCGTG TGTCTGTGAC 
TTTAGTTGCC AGAGTGTCCT TAAAAGCCCA GTGTGTGGCT CAGATGGAGT CACCTATAGC 
ACGGAGTGCC ATCTGAAGAA GGCCAGATGT GAAGCGCGGC AAGAGCTGTA CGTCGCTGCT 
CAGGGAGCCT GCCGGGGCCC TACCTTGGCT CCACTGCTAC CTATGGCCTC CCCACACTGT 
GCCCAAACCC CCTATGGCTG CTGCCAGGAC AATGTCACTG CTGCCCAGGG TGTGGGCTTG 
GCTGGCTGTC CCAGCACCTG CCATTGCAAC CCACACGGCT CCTATAGCGG CACTTGTGAC 
CCAGTCACAG GGCAGTGCTC CTGCCGACCA GGTGTAGGAG GCCTCAGGTG TGATCGCTGT 
GAGCCTGGCT TCTGGAACTT CCGTGGCATT GTCACCGATG GACATAGTGG TTGCACTCCC 
TGCAGCTGTG ACCCTCGGGG TGCTGTAAGA GATGACTGTG AGCAGATGAC TGGATTGTGT 
TCCTGTAGAC CTGGTGTGGC TGGTCCCAAG TGTGGGCAGT GTCCAGATGG TCAAGCCCTG 
GGCCATCTTG GCTGTGAAGC AGATCCCACA ACACCAGTGA CTTGTGTGGA AATGCACTGT 
GAGTTTGGCG CCTCCTGCGT AGAGGAGGCT GGTTTTGCCC AGTGTGTCTG CCCAACTCTC 
ACATGTCCAG AGGCTAACTC TACCAAGGTC TGTGGATCAG ATGGTGTCAC ATACGGCAAT 
GAATGCCAGC TGAAGACCAT TGCCTGCCGC CAGCGTCTGG ACATCTCCAT TCAGAGTCTT 
GGTCCATGCC GGGAGAGTGT TGCTCCTGGG GTTTCCCCTA CATCTGCATC TATGACCACC 
CCAAGGCATA TCCTGAGCAG GACACTGGCG TCTCCCCACA GCAGCCTTCC TCTGTCTCCC 
AGCACTACTG CCCATGATTG GCCCACCCCA TTACCCACAT CACCTCAGAC CGTAGTCGGC 
ACCCCCAGGA GCACTGCAGC CACACCCTCT GATGTGGCCA GTCTTGCTAC AGCGATCTTC 
AGGGAATCTG GCAGCACCAA CGGCAGTGGC GATGAGGAGC TCAGTGGCGA TGAGGAGGCC 
AGTGGGGGCG GGTCTGGGGG ACTTGAGCCC CCGGTGGGCA GCGTTGTGGT GACCCACGGG 
CCACCCATCG AGAGGGCTTC CTGTTACAAC TCACCTTTGG GCTGCTGCTC AGATGGCAAG 
ACACCCTCAC TGGACTCAGA AGGCTCCAAC TGTCCAGCTA CCAAGGCATT CCAGGGCGTG 
CTGGAGCTTG AGGGGGTCGA GGGACAGGAA CTGTTCTACA CACCAGAGAT GGCTGACCCC 
AAGTCAGAGT TGTTTGGGGA GACTGCAAGG AGCATTGAGA GCACGCTGGA CGACCTGTTC 
CGGAATTCGG ATGTTAAGAA GGACTTCTGG AGCATCCGCC TACGGGAACT GGGGCCTGGC 
AAATTAGTCC GTGCCATTGT GGATGTTCAC TTTGACCCCA CCACAGCCTT CCAGGCACCA 
GATGTGGGTC AGGCCTTGCT CCAACAGATC CAGGTATCCA GGCCGTGGGC CCTGGCAGTG 
AGGAGGCCTC TGCGGGAGCA TGTGCGATTC TTGGACTTTG ACTGGTTTCC CACTTTTTTT 
ACGGGAGCTG CAACAGGAAC CACAGCTGCT GTGGCCACAG CCAGAGCCAC CACTGTGAGC 
CGACTGTCTG CCTCTTCTGT CACCCCACGA GTCTACCCCA GTTACACCAG CCGGCCTGTT 
GGCAGAACTA CGGCACCGCT AACCACTCGC CGGCCACCAA CCACTACCGC CAGTATTGAC 
CGACCTCGGA CTCCAGGCCC GCAACGGCCC CCAAAGTCCT GTGATTCCCA GCCTTGCCTC 
CACGGAGGTA CCTGCCAGGA CCTGGATTCT GGCAAGGGTT TCAGCTGCAG CTGTACTGCA 
GGCAGGGCTG GCACTGTCTG TGAGAAAGTG CAGCTCCCCT CTGTGCCAGC TTTTAAGGGC 
CACTCCTTCT TGGCCTTCCC CACCCTCCGA GCCTACCACA CGCTGCGTCT GGCACTAGAA 
TTCCGGGCGC TGGAGACAGA GGGACTGCTG CTCTACAATG GCAATGCACG TGGCAAAGAT 
TTCCTGGCTC TGGCTCTGTT GGATGGTCAT GTACAGTTCA GGTTCGACAC GGGCTCAGGG 
CCGGCGGTGC TAACAAGCTT AGTGCCAGTG GAACCGGGAC GGTGGCACCG CCTCGAGTTG 
TCACGGCATT GGCGGCAGGG CACACTTTCT GTGGATGGCG AGGCTCCTGT TGTAGGTGAA 
AGTCCGAGTG GCACTGATGG CCTCAACTTG GACACGAAGC TCTATGTGGG TGGTCTCCCA 
GAAGAACAAG TTGCCACGGT GCTTGATCGG ACCTCTGTGG GCATCGGCCT GAAAGGATGC 
ATTCGTATGT TGGACATCAA CAACCAGCAG CTGGAGCTGA GCGATTGGCA GAGGGCTGTG 
GTTCAAAGCT CTGGTGTGGG GGAATGCGGA GACCATCCCT GCTCACCTAA CCCCTGCCAT 
GGCGGGGCCC TCTGCCAGGC CCTGGAGGCT GGCGTGTTCC TCTGTCAGTG CCCACCTGGC 
CGCTTTGGCC CAACTTGTGC AGATGAAAAG AACCCCTGCC AACCGAACCC CTGCCACGGG 
TCAGCCCCCT GCCATGTGCT TTCCAGGGGT GGGGCCAAGT GTGCGTGCCC CCTGGGACGC 
AGTGGTTCCT TCTGTGAGAC AGTCCTGGAG AATGCTGGCT CCCGGCCCTT CCTGGCTGAC 
TTTAATGGCT TCTCCTACCT GGAACTGAAA GGCTTGCACA CCTTCGAGAG AGACCTAGGG 
GAGAAGATGG CGCTGGAGAT GGTGTTCTTG GCTCGTGGGC CCAGTGGCTT ACTCCTCTAC 
AATGGGCAGA AGACGGATGG CAAGGGGGAC TTTGTATCCC TGGCCCTGCA TAACCGGCAC 
CTAGAGTTCC GCTATGACCT TGGCAAGGGG GCTGCAATCA TCAGGAGCAA AGAGCCCATA 
GCCCTGGGCA CCTGGGTTAG GGTATTCCTG GAACGAAATG GCCGCAAGGG TGCCCTTCAA 
GTGGGTGATG GGCCCCGTGT GCTAGGGGAA TCTCCGAAAT CCCGCAAGGT CCCGCACACC 
ATGCTCAACC TCAAGGAGCC CCTCTATGTG GGGGGAGCTC CTGACTTCAG CAAGCTGGCT 
CGGGGCGCTG CAGTGGCCTC CGGCTTTGAT GGTGCCATCC AGCTGGTGTC TCTAAGAGGC 
CATCAGCTGC TGACTCAGGA GCATGTGTTG CGGGCAGTAG ATGTAGCGCC TTTTGCAGGC 
CACCCTTGTA CCCAGGCCGT GGACAACCCC TGCCTTAATG GGGGCTCCTG TATCCCGAGG 
GAAGCCACTT ATGAGTGCCT GTGTCCTGGG GGCTTCTCTG GGCTGCACTG CGAGAAGGGG 
ATAGTTGAGA AGTCAGTGGG GGACCTAGAA ACACTGGCCT TTGATGGGCG GACCTACATC 
GAGTACCTCA ATGCTGTGAC TGAGAGCGAG CTGACCAATG AGATCCCAGC CCCCGAAACT 
CTGGATTCCC GGGCCCTTTT CAGTGAGAAA GCGCTGCAGA GCAACCACTT TGAGCTGAGC 
TTACGCACTG AGGCCACGCA GGGGCTGGTG CTGTGGATTG GAAAGGTTGG AGAACGTGCA 
GACTACATGG CTCTGGCCAT TGTGGATGGG CACCTACAAC TGAGCTATGA CCTAGGCTCC 
CAGCCAGTTG TGCTGCGCTC CACTGTGAAG GTCAACACCA ACCGCTGGCT TCGAGTCAGG 
GCTCACAGGG AGCACAGGGA AGGTTCCCTT CAGGTGGGCA ATGAAGCCCC TGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAATTGGAC ACAGATGGAG CCCTGTGGCT TGGAGGCCTA 
CAGAAGCTTC CTGTGGGGCA GGCTCTCCCC AAGGCCTATG GCACGGGTTT TGTGGGCTGT 
CTGCGGGACG TGGTAGTGGG CCATCGCCAG CTGCATCTGC TGGAGGACGC TGTCACCAAA 
CCAGAGCTAA GACCCTGCCC CACTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538559.1
CDS	99..5768
Translation	MPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538496.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538496.4

ORF Insert Sequence:

ATGCCTCCTG ATGTGTGCAG GGGAATGTTA TGTGGCTTTG GTGCTGTGTG TGAACCTAGT 
GTTGAGGATC CAGGCCGGGC CTCCTGTGTG TGCAAGAAGA ATGTCTGCCC TGCTATGGTA 
GCTCCTGTGT GTGGCTCAGA TGCTTCCACC TATAGCAACG AGTGTGAGCT ACAGCGTGCA 
CAGTGCAACC AGCAACGGCG CATCCGCCTA CTCCGCCAAG GGCCATGTGG GTCCCGGGAC 
CCCTGTGCCA ATGTGACCTG CAGTTTCGGT AGTACCTGTG TACCTTCAGC CGATGGACAG 
ACCGCCTCGT GTCTGTGTCC TACAACCTGC TTTGGGGCCC CTGATGGCAC AGTGTGTGGC 
AGTGATGGTG TTGACTACCC TAGTGAGTGC CAGCTGCTCC GTCATGCCTG TGCCAACCAG 
GAGCACATCT TTAAGAAGTT CGATGGTCCT TGTGACCCCT GCCAGGGCAG CATGTCAGAC 
CTGAATCATA TTTGCCGGGT GAACCCACGT ACACGGCACC CAGAAATGCT TCTGCGGCCT 
GAGAACTGCC CCGCCCAACA CACACCTATC TGTGGAGATG ATGGGGTCAC CTATGAAAAC 
GACTGTGTCA TGAGCCGTAT AGGTGCAGCC CGTGGCCTGC TTCTCCAGAA AGTGCGCTCT 
GGTCAATGCC AGACTCGAGA CCAGTGCCCG GAGACCTGCC AGTTTAACTC TGTATGCCTG 
TCCCGCCGCG GCCGTCCCCA CTGTTCCTGC GATCGCGTCA CCTGTGATGG GGCTTACAGG 
CCAGTGTGTG CCCAGGATGG GCACACGTAT GACAATGACT GTTGGCGCCA ACAGGCCGAG 
TGTCGACAAC AGCAGACCAT TCCCCCCAAG CACCAGGGCC CGTGTGACCA GACCCCATCC 
CCGTGCCGTG GAGCGCAGTG TGCATTTGGG GCAACATGCA CAGTGAAGAA TGGGAAAGCT 
GTGTGCGAGT GCCAGCGGGT GTGCTCGGGC GGCTACGATC CTGTGTGCGG CAGTGATGGT 
GTCACTTACG GCAGTGTGTG CGAGCTGGAA TCCATGGCCT GTACCCTTGG GCGGGAAATC 
CGAGTGGCCC GCAGAGGACC GTGTGACCGA TGTGGGCAGT GCCGGTTTGG ATCCTTGTGC 
GAGGTGGAGA CTGGACGCTG TGTGTGCCCC TCTGAGTGTG TGGAGTCAGC CCAGCCCGTA 
TGTGGCTCTG ACGGACACAC ATATGCTAGT GAATGTGAGC TGCATGTCCA CGCCTGTACA 
CACCAGATCA GCCTATACGT GGCCTCAGCC GGACACTGCC AGACCTGTGG AGAAACAGTT 
TGTACCTTTG GGGCTGTGTG CTCAGCTGGA CAGTGTGTAT GTCCCCGTTG TGAGCACCCC 
CCACCTGGCC CTGTGTGCGG CAGTGATGGC GTCACCTACC TCAGTGCCTG TGAGCTCCGA 
GAGGCTGCCT GTCAGCAGCA GGTACAAATT GAGGAGGCCC GTGCAGGGCC GTGTGAGCCG 
GCTGAGTGTG GCTCAGGGGG CTCTGGGTCT GGGGAAGACA ATGCGTGTGA GCAGGAGCTG 
TGTCGGCAGC ATGGTGGTGT CTGGGATGAG GACTCAGAAG ACGGGCCGTG TGTCTGTGAC 
TTTAGTTGCC AGAGTGTCCT TAAAAGCCCA GTGTGTGGCT CAGATGGAGT CACCTATAGC 
ACGGAGTGCC ATCTGAAGAA GGCCAGATGT GAAGCGCGGC AAGAGCTGTA CGTCGCTGCT 
CAGGGAGCCT GCCGGGGCCC TACCTTGGCT CCACTGCTAC CTATGGCCTC CCCACACTGT 
GCCCAAACCC CCTATGGCTG CTGCCAGGAC AATGTCACTG CTGCCCAGGG TGTGGGCTTG 
GCTGGCTGTC CCAGCACCTG CCATTGCAAC CCACACGGCT CCTATAGCGG CACTTGTGAC 
CCAGTCACAG GGCAGTGCTC CTGCCGACCA GGTGTAGGAG GCCTCAGGTG TGATCGCTGT 
GAGCCTGGCT TCTGGAACTT CCGTGGCATT GTCACCGATG GACATAGTGG TTGCACTCCC 
TGCAGCTGTG ACCCTCGGGG TGCTGTAAGA GATGACTGTG AGCAGATGAC TGGATTGTGT 
TCCTGTAGAC CTGGTGTGGC TGGTCCCAAG TGTGGGCAGT GTCCAGATGG TCAAGCCCTG 
GGCCATCTTG GCTGTGAAGC AGATCCCACA ACACCAGTGA CTTGTGTGGA AATGCACTGT 
GAGTTTGGCG CCTCCTGCGT AGAGGAGGCT GGTTTTGCCC AGTGTGTCTG CCCAACTCTC 
ACATGTCCAG AGGCTAACTC TACCAAGGTC TGTGGATCAG ATGGTGTCAC ATACGGCAAT 
GAATGCCAGC TGAAGACCAT TGCCTGCCGC CAGCGTCTGG ACATCTCCAT TCAGAGTCTT 
GGTCCATGCC GGGAGAGTGT TGCTCCTGGG GTTTCCCCTA CATCTGCATC TATGACCACC 
CCAAGGCATA TCCTGAGCAG GACACTGGCG TCTCCCCACA GCAGCCTTCC TCTGTCTCCC 
AGCACTACTG CCCATGATTG GCCCACCCCA TTACCCACAT CACCTCAGAC CGTAGTCGGC 
ACCCCCAGGA GCACTGCAGC CACACCCTCT GATGTGGCCA GTCTTGCTAC AGCGATCTTC 
AGGGAATCTG GCAGCACCAA CGGCAGTGGC GATGAGGAGC TCAGTGGCGA TGAGGAGGCC 
AGTGGGGGCG GGTCTGGGGG ACTTGAGCCC CCGGTGGGCA GCGTTGTGGT GACCCACGGG 
CCACCCATCG AGAGGGCTTC CTGTTACAAC TCACCTTTGG GCTGCTGCTC AGATGGCAAG 
ACACCCTCAC TGGACTCAGA AGGCTCCAAC TGTCCAGCTA CCAAGGCATT CCAGGGCGTG 
CTGGAGCTTG AGGGGGTCGA GGGACAGGAA CTGTTCTACA CACCAGAGAT GGCTGACCCC 
AAGTCAGAGT TGTTTGGGGA GACTGCAAGG AGCATTGAGA GCACGCTGGA CGACCTGTTC 
CGGAATTCGG ATGTTAAGAA GGACTTCTGG AGCATCCGCC TACGGGAACT GGGGCCTGGC 
AAATTAGTCC GTGCCATTGT GGATGTTCAC TTTGACCCCA CCACAGCCTT CCAGGCACCA 
GATGTGGGTC AGGCCTTGCT CCAACAGATC CAGGTATCCA GGCCGTGGGC CCTGGCAGTG 
AGGAGGCCTC TGCGGGAGCA TGTGCGATTC TTGGACTTTG ACTGGTTTCC CACTTTTTTT 
ACGGGAGCTG CAACAGGAAC CACAGCTGCT GTGGCCACAG CCAGAGCCAC CACTGTGAGC 
CGACTGTCTG CCTCTTCTGT CACCCCACGA GTCTACCCCA GTTACACCAG CCGGCCTGTT 
GGCAGAACTA CGGCACCGCT AACCACTCGC CGGCCACCAA CCACTACCGC CAGTATTGAC 
CGACCTCGGA CTCCAGGCCC GCAACGGCCC CCAAAGTCCT GTGATTCCCA GCCTTGCCTC 
CACGGAGGTA CCTGCCAGGA CCTGGATTCT GGCAAGGGTT TCAGCTGCAG CTGTACTGCA 
GGCAGGGCTG GCACTGTCTG TGAGAAAGTG CAGCTCCCCT CTGTGCCAGC TTTTAAGGGC 
CACTCCTTCT TGGCCTTCCC CACCCTCCGA GCCTACCACA CGCTGCGTCT GGCACTAGAA 
TTCCGGGCGC TGGAGACAGA GGGACTGCTG CTCTACAATG GCAATGCACG TGGCAAAGAT 
TTCCTGGCTC TGGCTCTGTT GGATGGTCAT GTACAGTTCA GGTTCGACAC GGGCTCAGGG 
CCGGCGGTGC TAACAAGCTT AGTGCCAGTG GAACCGGGAC GGTGGCACCG CCTCGAGTTG 
TCACGGCATT GGCGGCAGGG CACACTTTCT GTGGATGGCG AGGCTCCTGT TGTAGGTGAA 
AGTCCGAGTG GCACTGATGG CCTCAACTTG GACACGAAGC TCTATGTGGG TGGTCTCCCA 
GAAGAACAAG TTGCCACGGT GCTTGATCGG ACCTCTGTGG GCATCGGCCT GAAAGGATGC 
ATTCGTATGT TGGACATCAA CAACCAGCAG CTGGAGCTGA GCGATTGGCA GAGGGCTGTG 
GTTCAAAGCT CTGGTGTGGG GGAATGCGGA GACCATCCCT GCTCACCTAA CCCCTGCCAT 
GGCGGGGCCC TCTGCCAGGC CCTGGAGGCT GGCGTGTTCC TCTGTCAGTG CCCACCTGGC 
CGCTTTGGCC CAACTTGTGC AGATGAAAAG AACCCCTGCC AACCGAACCC CTGCCACGGG 
TCAGCCCCCT GCCATGTGCT TTCCAGGGGT GGGGCCAAGT GTGCGTGCCC CCTGGGACGC 
AGTGGTTCCT TCTGTGAGAC AGTCCTGGAG AATGCTGGCT CCCGGCCCTT CCTGGCTGAC 
TTTAATGGCT TCTCCTACCT GGAACTGAAA GGCTTGCACA CCTTCGAGAG AGACCTAGGG 
GAGAAGATGG CGCTGGAGAT GGTGTTCTTG GCTCGTGGGC CCAGTGGCTT ACTCCTCTAC 
AATGGGCAGA AGACGGATGG CAAGGGGGAC TTTGTATCCC TGGCCCTGCA TAACCGGCAC 
CTAGAGTTCC GCTATGACCT TGGCAAGGGG GCTGCAATCA TCAGGAGCAA AGAGCCCATA 
GCCCTGGGCA CCTGGGTTAG GGTATTCCTG GAACGAAATG GCCGCAAGGG TGCCCTTCAA 
GTGGGTGATG GGCCCCGTGT GCTAGGGGAA TCTCCGAAAT CCCGCAAGGT CCCGCACACC 
ATGCTCAACC TCAAGGAGCC CCTCTATGTG GGGGGAGCTC CTGACTTCAG CAAGCTGGCT 
CGGGGCGCTG CAGTGGCCTC CGGCTTTGAT GGTGCCATCC AGCTGGTGTC TCTAAGAGGC 
CATCAGCTGC TGACTCAGGA GCATGTGTTG CGGGCAGTAG ATGTAGCGCC TTTTGCAGGC 
CACCCTTGTA CCCAGGCCGT GGACAACCCC TGCCTTAATG GGGGCTCCTG TATCCCGAGG 
GAAGCCACTT ATGAGTGCCT GTGTCCTGGG GGCTTCTCTG GGCTGCACTG CGAGAAGGGG 
ATAGTTGAGA AGTCAGTGGG GGACCTAGAA ACACTGGCCT TTGATGGGCG GACCTACATC 
GAGTACCTCA ATGCTGTGAC TGAGAGCGAG CTGACCAATG AGATCCCAGC CCCCGAAACT 
CTGGATTCCC GGGCCCTTTT CAGTGAGAAA GCGCTGCAGA GCAACCACTT TGAGCTGAGC 
TTACGCACTG AGGCCACGCA GGGGCTGGTG CTGTGGATTG GAAAGGTTGG AGAACGTGCA 
GACTACATGG CTCTGGCCAT TGTGGATGGG CACCTACAAC TGAGCTATGA CCTAGGCTCC 
CAGCCAGTTG TGCTGCGCTC CACTGTGAAG GTCAACACCA ACCGCTGGCT TCGAGTCAGG 
GCTCACAGGG AGCACAGGGA AGGTTCCCTT CAGGTGGGCA ATGAAGCCCC TGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAATTGGAC ACAGATGGAG CCCTGTGGCT TGGAGGCCTA 
CAGAAGCTTC CTGTGGGGCA GGCTCTCCCC AAGGCCTATG GCACGGGTTT TGTGGGCTGT 
CTGCGGGACG TGGTAGTGGG CCATCGCCAG CTGCATCTGC TGGAGGACGC TGTCACCAAA 
CCAGAGCTAA GACCCTGCCC CACTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538491.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538554.1 Mus musculus agrin (Agrn), transcript variant X1, mRNA.

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CloneID	OMu33953
Clone ID Related Accession (Same CDS sequence)	XM_006538491.1 , XM_006538491.2
Accession Version	XM_006538491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CGAGCTGACC 
AATGAGATCC CAGCCCCCGA AACTCTGGAT TCCCGGGCCC TTTTCAGTGA GAAAGCGCTG 
CAGAGCAACC ACTTTGAGCT GAGCTTACGC ACTGAGGCCA CGCAGGGGCT GGTGCTGTGG 
ATTGGAAAGG TTGGAGAACG TGCAGACTAC ATGGCTCTGG CCATTGTGGA TGGGCACCTA 
CAACTGAGCT ATGACCTAGG CTCCCAGCCA GTTGTGCTGC GCTCCACTGT GAAGGTCAAC 
ACCAACCGCT GGCTTCGAGT CAGGGCTCAC AGGGAGCACA GGGAAGGTTC CCTTCAGGTG 
GGCAATGAAG CCCCTGTGAC TGGCTCTTCC CCGCTGGGTG CCACACAATT GGACACAGAT 
GGAGCCCTGT GGCTTGGAGG CCTACAGAAG CTTCCTGTGG GGCAGGCTCT CCCCAAGGCC 
TATGGCACGG GTTTTGTGGG CTGTCTGCGG GACGTGGTAG TGGGCCATCG CCAGCTGCAT 
CTGCTGGAGG ACGCTGTCAC CAAACCAGAG CTAAGACCCT GCCCCACTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538554.1
CDS	114..6287
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538491.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538491.2

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CGAGCTGACC 
AATGAGATCC CAGCCCCCGA AACTCTGGAT TCCCGGGCCC TTTTCAGTGA GAAAGCGCTG 
CAGAGCAACC ACTTTGAGCT GAGCTTACGC ACTGAGGCCA CGCAGGGGCT GGTGCTGTGG 
ATTGGAAAGG TTGGAGAACG TGCAGACTAC ATGGCTCTGG CCATTGTGGA TGGGCACCTA 
CAACTGAGCT ATGACCTAGG CTCCCAGCCA GTTGTGCTGC GCTCCACTGT GAAGGTCAAC 
ACCAACCGCT GGCTTCGAGT CAGGGCTCAC AGGGAGCACA GGGAAGGTTC CCTTCAGGTG 
GGCAATGAAG CCCCTGTGAC TGGCTCTTCC CCGCTGGGTG CCACACAATT GGACACAGAT 
GGAGCCCTGT GGCTTGGAGG CCTACAGAAG CTTCCTGTGG GGCAGGCTCT CCCCAAGGCC 
TATGGCACGG GTTTTGTGGG CTGTCTGCGG GACGTGGTAG TGGGCCATCG CCAGCTGCAT 
CTGCTGGAGG ACGCTGTCAC CAAACCAGAG CTAAGACCCT GCCCCACTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538492.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538555.1 Mus musculus agrin (Agrn), transcript variant X3, mRNA.

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CloneID	OMu33954
Clone ID Related Accession (Same CDS sequence)	XM_006538492.2 , XM_006538492.1
Accession Version	XM_006538492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CCCCGAAACT 
CTGGATTCCC GGGCCCTTTT CAGTGAGAAA GCGCTGCAGA GCAACCACTT TGAGCTGAGC 
TTACGCACTG AGGCCACGCA GGGGCTGGTG CTGTGGATTG GAAAGGTTGG AGAACGTGCA 
GACTACATGG CTCTGGCCAT TGTGGATGGG CACCTACAAC TGAGCTATGA CCTAGGCTCC 
CAGCCAGTTG TGCTGCGCTC CACTGTGAAG GTCAACACCA ACCGCTGGCT TCGAGTCAGG 
GCTCACAGGG AGCACAGGGA AGGTTCCCTT CAGGTGGGCA ATGAAGCCCC TGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAATTGGAC ACAGATGGAG CCCTGTGGCT TGGAGGCCTA 
CAGAAGCTTC CTGTGGGGCA GGCTCTCCCC AAGGCCTATG GCACGGGTTT TGTGGGCTGT 
CTGCGGGACG TGGTAGTGGG CCATCGCCAG CTGCATCTGC TGGAGGACGC TGTCACCAAA 
CCAGAGCTAA GACCCTGCCC CACTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538555.1
CDS	113..6262
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538492.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538492.2

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CCCCGAAACT 
CTGGATTCCC GGGCCCTTTT CAGTGAGAAA GCGCTGCAGA GCAACCACTT TGAGCTGAGC 
TTACGCACTG AGGCCACGCA GGGGCTGGTG CTGTGGATTG GAAAGGTTGG AGAACGTGCA 
GACTACATGG CTCTGGCCAT TGTGGATGGG CACCTACAAC TGAGCTATGA CCTAGGCTCC 
CAGCCAGTTG TGCTGCGCTC CACTGTGAAG GTCAACACCA ACCGCTGGCT TCGAGTCAGG 
GCTCACAGGG AGCACAGGGA AGGTTCCCTT CAGGTGGGCA ATGAAGCCCC TGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAATTGGAC ACAGATGGAG CCCTGTGGCT TGGAGGCCTA 
CAGAAGCTTC CTGTGGGGCA GGCTCTCCCC AAGGCCTATG GCACGGGTTT TGTGGGCTGT 
CTGCGGGACG TGGTAGTGGG CCATCGCCAG CTGCATCTGC TGGAGGACGC TGTCACCAAA 
CCAGAGCTAA GACCCTGCCC CACTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538493.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538556.1 Mus musculus agrin (Agrn), transcript variant X4, mRNA.

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CloneID	OMu33955
Clone ID Related Accession (Same CDS sequence)	XM_006538493.1 , XM_006538493.2
Accession Version	XM_006538493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CGAGCTGACC 
AATGAGATCC CAGCTGAGAA AGCGCTGCAG AGCAACCACT TTGAGCTGAG CTTACGCACT 
GAGGCCACGC AGGGGCTGGT GCTGTGGATT GGAAAGGTTG GAGAACGTGC AGACTACATG 
GCTCTGGCCA TTGTGGATGG GCACCTACAA CTGAGCTATG ACCTAGGCTC CCAGCCAGTT 
GTGCTGCGCT CCACTGTGAA GGTCAACACC AACCGCTGGC TTCGAGTCAG GGCTCACAGG 
GAGCACAGGG AAGGTTCCCT TCAGGTGGGC AATGAAGCCC CTGTGACTGG CTCTTCCCCG 
CTGGGTGCCA CACAATTGGA CACAGATGGA GCCCTGTGGC TTGGAGGCCT ACAGAAGCTT 
CCTGTGGGGC AGGCTCTCCC CAAGGCCTAT GGCACGGGTT TTGTGGGCTG TCTGCGGGAC 
GTGGTAGTGG GCCATCGCCA GCTGCATCTG CTGGAGGACG CTGTCACCAA ACCAGAGCTA 
AGACCCTGCC CCACTCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538556.1
CDS	113..6253
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538493.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538493.2

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CGAGCTGACC 
AATGAGATCC CAGCTGAGAA AGCGCTGCAG AGCAACCACT TTGAGCTGAG CTTACGCACT 
GAGGCCACGC AGGGGCTGGT GCTGTGGATT GGAAAGGTTG GAGAACGTGC AGACTACATG 
GCTCTGGCCA TTGTGGATGG GCACCTACAA CTGAGCTATG ACCTAGGCTC CCAGCCAGTT 
GTGCTGCGCT CCACTGTGAA GGTCAACACC AACCGCTGGC TTCGAGTCAG GGCTCACAGG 
GAGCACAGGG AAGGTTCCCT TCAGGTGGGC AATGAAGCCC CTGTGACTGG CTCTTCCCCG 
CTGGGTGCCA CACAATTGGA CACAGATGGA GCCCTGTGGC TTGGAGGCCT ACAGAAGCTT 
CCTGTGGGGC AGGCTCTCCC CAAGGCCTAT GGCACGGGTT TTGTGGGCTG TCTGCGGGAC 
GTGGTAGTGG GCCATCGCCA GCTGCATCTG CTGGAGGACG CTGTCACCAA ACCAGAGCTA 
AGACCCTGCC CCACTCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538494.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538557.1 Mus musculus agrin (Agrn), transcript variant X5, mRNA.

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CloneID	OMu33956
Clone ID Related Accession (Same CDS sequence)	XM_006538494.3 , XM_006538494.4
Accession Version	XM_006538494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG TGAGAAAGCG 
CTGCAGAGCA ACCACTTTGA GCTGAGCTTA CGCACTGAGG CCACGCAGGG GCTGGTGCTG 
TGGATTGGAA AGGTTGGAGA ACGTGCAGAC TACATGGCTC TGGCCATTGT GGATGGGCAC 
CTACAACTGA GCTATGACCT AGGCTCCCAG CCAGTTGTGC TGCGCTCCAC TGTGAAGGTC 
AACACCAACC GCTGGCTTCG AGTCAGGGCT CACAGGGAGC ACAGGGAAGG TTCCCTTCAG 
GTGGGCAATG AAGCCCCTGT GACTGGCTCT TCCCCGCTGG GTGCCACACA ATTGGACACA 
GATGGAGCCC TGTGGCTTGG AGGCCTACAG AAGCTTCCTG TGGGGCAGGC TCTCCCCAAG 
GCCTATGGCA CGGGTTTTGT GGGCTGTCTG CGGGACGTGG TAGTGGGCCA TCGCCAGCTG 
CATCTGCTGG AGGACGCTGT CACCAAACCA GAGCTAAGAC CCTGCCCCAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538557.1
CDS	113..6229
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538494.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538494.4

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG TGAGAAAGCG 
CTGCAGAGCA ACCACTTTGA GCTGAGCTTA CGCACTGAGG CCACGCAGGG GCTGGTGCTG 
TGGATTGGAA AGGTTGGAGA ACGTGCAGAC TACATGGCTC TGGCCATTGT GGATGGGCAC 
CTACAACTGA GCTATGACCT AGGCTCCCAG CCAGTTGTGC TGCGCTCCAC TGTGAAGGTC 
AACACCAACC GCTGGCTTCG AGTCAGGGCT CACAGGGAGC ACAGGGAAGG TTCCCTTCAG 
GTGGGCAATG AAGCCCCTGT GACTGGCTCT TCCCCGCTGG GTGCCACACA ATTGGACACA 
GATGGAGCCC TGTGGCTTGG AGGCCTACAG AAGCTTCCTG TGGGGCAGGC TCTCCCCAAG 
GCCTATGGCA CGGGTTTTGT GGGCTGTCTG CGGGACGTGG TAGTGGGCCA TCGCCAGCTG 
CATCTGCTGG AGGACGCTGT CACCAAACCA GAGCTAAGAC CCTGCCCCAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_011250176.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248478.1 Mus musculus agrin (Agrn), transcript variant X2, mRNA.

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CloneID	OMu67084
Clone ID Related Accession (Same CDS sequence)	XM_011250176.2 , XM_011250176.1
Accession Version	XM_011250176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
GTCCCGCACA CCATGCTCAA CCTCAAGGAG CCCCTCTATG TGGGGGGAGC TCCTGACTTC 
AGCAAGCTGG CTCGGGGCGC TGCAGTGGCC TCCGGCTTTG ATGGTGCCAT CCAGCTGGTG 
TCTCTAAGAG GCCATCAGCT GCTGACTCAG GAGCATGTGT TGCGGGCAGT AGATGTAGCG 
CCTTTTGCAG GCCACCCTTG TACCCAGGCC GTGGACAACC CCTGCCTTAA TGGGGGCTCC 
TGTATCCCGA GGGAAGCCAC TTATGAGTGC CTGTGTCCTG GGGGCTTCTC TGGGCTGCAC 
TGCGAGAAGG GGATAGTTGA GAAGTCAGTG GGGGACCTAG AAACACTGGC CTTTGATGGG 
CGGACCTACA TCGAGTACCT CAATGCTGTG ACTGAGAGCG AGCTGACCAA TGAGATCCCA 
GCCCCCGAAA CTCTGGATTC CCGGGCCCTT TTCAGTGAGA AAGCGCTGCA GAGCAACCAC 
TTTGAGCTGA GCTTACGCAC TGAGGCCACG CAGGGGCTGG TGCTGTGGAT TGGAAAGGTT 
GGAGAACGTG CAGACTACAT GGCTCTGGCC ATTGTGGATG GGCACCTACA ACTGAGCTAT 
GACCTAGGCT CCCAGCCAGT TGTGCTGCGC TCCACTGTGA AGGTCAACAC CAACCGCTGG 
CTTCGAGTCA GGGCTCACAG GGAGCACAGG GAAGGTTCCC TTCAGGTGGG CAATGAAGCC 
CCTGTGACTG GCTCTTCCCC GCTGGGTGCC ACACAATTGG ACACAGATGG AGCCCTGTGG 
CTTGGAGGCC TACAGAAGCT TCCTGTGGGG CAGGCTCTCC CCAAGGCCTA TGGCACGGGT 
TTTGTGGGCT GTCTGCGGGA CGTGGTAGTG GGCCATCGCC AGCTGCATCT GCTGGAGGAC 
GCTGTCACCA AACCAGAGCT AAGACCCTGC CCCACTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248478.1
CDS	113..6274
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_011250176.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250176.2

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
GTCCCGCACA CCATGCTCAA CCTCAAGGAG CCCCTCTATG TGGGGGGAGC TCCTGACTTC 
AGCAAGCTGG CTCGGGGCGC TGCAGTGGCC TCCGGCTTTG ATGGTGCCAT CCAGCTGGTG 
TCTCTAAGAG GCCATCAGCT GCTGACTCAG GAGCATGTGT TGCGGGCAGT AGATGTAGCG 
CCTTTTGCAG GCCACCCTTG TACCCAGGCC GTGGACAACC CCTGCCTTAA TGGGGGCTCC 
TGTATCCCGA GGGAAGCCAC TTATGAGTGC CTGTGTCCTG GGGGCTTCTC TGGGCTGCAC 
TGCGAGAAGG GGATAGTTGA GAAGTCAGTG GGGGACCTAG AAACACTGGC CTTTGATGGG 
CGGACCTACA TCGAGTACCT CAATGCTGTG ACTGAGAGCG AGCTGACCAA TGAGATCCCA 
GCCCCCGAAA CTCTGGATTC CCGGGCCCTT TTCAGTGAGA AAGCGCTGCA GAGCAACCAC 
TTTGAGCTGA GCTTACGCAC TGAGGCCACG CAGGGGCTGG TGCTGTGGAT TGGAAAGGTT 
GGAGAACGTG CAGACTACAT GGCTCTGGCC ATTGTGGATG GGCACCTACA ACTGAGCTAT 
GACCTAGGCT CCCAGCCAGT TGTGCTGCGC TCCACTGTGA AGGTCAACAC CAACCGCTGG 
CTTCGAGTCA GGGCTCACAG GGAGCACAGG GAAGGTTCCC TTCAGGTGGG CAATGAAGCC 
CCTGTGACTG GCTCTTCCCC GCTGGGTGCC ACACAATTGG ACACAGATGG AGCCCTGTGG 
CTTGGAGGCC TACAGAAGCT TCCTGTGGGG CAGGCTCTCC CCAAGGCCTA TGGCACGGGT 
TTTGTGGGCT GTCTGCGGGA CGTGGTAGTG GGCCATCGCC AGCTGCATCT GCTGGAGGAC 
GCTGTCACCA AACCAGAGCT AAGACCCTGC CCCACTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( NM_021604.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067617.3 Mus musculus agrin (Agrn), transcript variant 1, mRNA.

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CloneID	OMu12494
Clone ID Related Accession (Same CDS sequence)	NM_021604.3
Accession Version	NM_021604.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	agrin isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT998563.5 and AL928667.4. On Aug 25, 2011 this sequence version replaced NM_021604.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. The combination of the first three exons is inferred based on available transcript data and further supported by similar exon-intron structure of the related human gene, GeneID 375790. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
GTCCCGCACA CCATGCTCAA CCTCAAGGAG CCCCTCTATG TGGGGGGAGC TCCTGACTTC 
AGCAAGCTGG CTCGGGGCGC TGCAGTGGCC TCCGGCTTTG ATGGTGCCAT CCAGCTGGTG 
TCTCTAAGAG GCCATCAGCT GCTGACTCAG GAGCATGTGT TGCGGGCAGT AGATGTAGCG 
CCTTTTGCAG GCCACCCTTG TACCCAGGCC GTGGACAACC CCTGCCTTAA TGGGGGCTCC 
TGTATCCCGA GGGAAGCCAC TTATGAGTGC CTGTGTCCTG GGGGCTTCTC TGGGCTGCAC 
TGCGAGAAGG GGATAGTTGA GAAGTCAGTG GGGGACCTAG AAACACTGGC CTTTGATGGG 
CGGACCTACA TCGAGTACCT CAATGCTGTG ACTGAGAGTG AGAAAGCGCT GCAGAGCAAC 
CACTTTGAGC TGAGCTTACG CACTGAGGCC ACGCAGGGGC TGGTGCTGTG GATTGGAAAG 
GTTGGAGAAC GTGCAGACTA CATGGCTCTG GCCATTGTGG ATGGGCACCT ACAACTGAGC 
TATGACCTAG GCTCCCAGCC AGTTGTGCTG CGCTCCACTG TGAAGGTCAA CACCAACCGC 
TGGCTTCGAG TCAGGGCTCA CAGGGAGCAC AGGGAAGGTT CCCTTCAGGT GGGCAATGAA 
GCCCCTGTGA CTGGCTCTTC CCCGCTGGGT GCCACACAAT TGGACACAGA TGGAGCCCTG 
TGGCTTGGAG GCCTACAGAA GCTTCCTGTG GGGCAGGCTC TCCCCAAGGC CTATGGCACG 
GGTTTTGTGG GCTGTCTGCG GGACGTGGTA GTGGGCCATC GCCAGCTGCA TCTGCTGGAG 
GACGCTGTCA CCAAACCAGA GCTAAGACCC TGCCCCACTC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067617.3
CDS	69..6173
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	NM_021604.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021604.3

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
GTCCCGCACA CCATGCTCAA CCTCAAGGAG CCCCTCTATG TGGGGGGAGC TCCTGACTTC 
AGCAAGCTGG CTCGGGGCGC TGCAGTGGCC TCCGGCTTTG ATGGTGCCAT CCAGCTGGTG 
TCTCTAAGAG GCCATCAGCT GCTGACTCAG GAGCATGTGT TGCGGGCAGT AGATGTAGCG 
CCTTTTGCAG GCCACCCTTG TACCCAGGCC GTGGACAACC CCTGCCTTAA TGGGGGCTCC 
TGTATCCCGA GGGAAGCCAC TTATGAGTGC CTGTGTCCTG GGGGCTTCTC TGGGCTGCAC 
TGCGAGAAGG GGATAGTTGA GAAGTCAGTG GGGGACCTAG AAACACTGGC CTTTGATGGG 
CGGACCTACA TCGAGTACCT CAATGCTGTG ACTGAGAGTG AGAAAGCGCT GCAGAGCAAC 
CACTTTGAGC TGAGCTTACG CACTGAGGCC ACGCAGGGGC TGGTGCTGTG GATTGGAAAG 
GTTGGAGAAC GTGCAGACTA CATGGCTCTG GCCATTGTGG ATGGGCACCT ACAACTGAGC 
TATGACCTAG GCTCCCAGCC AGTTGTGCTG CGCTCCACTG TGAAGGTCAA CACCAACCGC 
TGGCTTCGAG TCAGGGCTCA CAGGGAGCAC AGGGAAGGTT CCCTTCAGGT GGGCAATGAA 
GCCCCTGTGA CTGGCTCTTC CCCGCTGGGT GCCACACAAT TGGACACAGA TGGAGCCCTG 
TGGCTTGGAG GCCTACAGAA GCTTCCTGTG GGGCAGGCTC TCCCCAAGGC CTATGGCACG 
GGTTTTGTGG GCTGTCTGCG GGACGTGGTA GTGGGCCATC GCCAGCTGCA TCTGCTGGAG 
GACGCTGTCA CCAAACCAGA GCTAAGACCC TGCCCCACTC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( NM_001369026.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355955.1 Mus musculus agrin (Agrn), transcript variant 2, mRNA.

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CloneID	OMu67085
Clone ID Related Accession (Same CDS sequence)	XM_011250177.2 , NM_001369026.1
Accession Version	NM_001369026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	agrin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT998563.5 and AL928667.4. On Mar 12, 2019 this sequence version replaced XM_011250177.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGCAGC CTGGTGCCTC CGTGCTGGTT 
CGGTACTTCA TGATCCCCTG CAACATCTGC TTGATCCTCT TGGCTACTTC TACGTTGGGC 
TTTGCGGTGC TGCTTTTCCT CAGCAACTAC AAACCTGGGA TCCACTTCAC AGCAGCGCCT 
TCTATGCCTC CTGATGTGTG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG GGCCTCCTGT GTGTGCAAGA AGAATGTCTG CCCTGCTATG 
GTAGCTCCTG TGTGTGGCTC AGATGCTTCC ACCTATAGCA ACGAGTGTGA GCTACAGCGT 
GCACAGTGCA ACCAGCAACG GCGCATCCGC CTACTCCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAATGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC AGCCGATGGA 
CAGACCGCCT CGTGTCTGTG TCCTACAACC TGCTTTGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGATG GTGTTGACTA CCCTAGTGAG TGCCAGCTGC TCCGTCATGC CTGTGCCAAC 
CAGGAGCACA TCTTTAAGAA GTTCGATGGT CCTTGTGACC CCTGCCAGGG CAGCATGTCA 
GACCTGAATC ATATTTGCCG GGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCCGCCCA ACACACACCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA GCCCGTGGCC TGCTTCTCCA GAAAGTGCGC 
TCTGGTCAAT GCCAGACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCTGTATGC 
CTGTCCCGCC GCGGCCGTCC CCACTGTTCC TGCGATCGCG TCACCTGTGA TGGGGCTTAC 
AGGCCAGTGT GTGCCCAGGA TGGGCACACG TATGACAATG ACTGTTGGCG CCAACAGGCC 
GAGTGTCGAC AACAGCAGAC CATTCCCCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCA 
TCCCCGTGCC GTGGAGCGCA GTGTGCATTT GGGGCAACAT GCACAGTGAA GAATGGGAAA 
GCTGTGTGCG AGTGCCAGCG GGTGTGCTCG GGCGGCTACG ATCCTGTGTG CGGCAGTGAT 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCGAGTGG CCCGCAGAGG ACCGTGTGAC CGATGTGGGC AGTGCCGGTT TGGATCCTTG 
TGCGAGGTGG AGACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGTGGCT CTGACGGACA CACATATGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACACACCAGA TCAGCCTATA CGTGGCCTCA GCCGGACACT GCCAGACCTG TGGAGAAACA 
GTTTGTACCT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCCCCACCTG GCCCTGTGTG CGGCAGTGAT GGCGTCACCT ACCTCAGTGC CTGTGAGCTC 
CGAGAGGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCGTGCAGG GCCGTGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGG TCTGGGGAAG ACAATGCGTG TGAGCAGGAG 
CTGTGTCGGC AGCATGGTGG TGTCTGGGAT GAGGACTCAG AAGACGGGCC GTGTGTCTGT 
GACTTTAGTT GCCAGAGTGT CCTTAAAAGC CCAGTGTGTG GCTCAGATGG AGTCACCTAT 
AGCACGGAGT GCCATCTGAA GAAGGCCAGA TGTGAAGCGC GGCAAGAGCT GTACGTCGCT 
GCTCAGGGAG CCTGCCGGGG CCCTACCTTG GCTCCACTGC TACCTATGGC CTCCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATGTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCACACG GCTCCTATAG CGGCACTTGT 
GACCCAGTCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GTGACCCTCG GGGTGCTGTA AGAGATGACT GTGAGCAGAT GACTGGATTG 
TGTTCCTGTA GACCTGGTGT GGCTGGTCCC AAGTGTGGGC AGTGTCCAGA TGGTCAAGCC 
CTGGGCCATC TTGGCTGTGA AGCAGATCCC ACAACACCAG TGACTTGTGT GGAAATGCAC 
TGTGAGTTTG GCGCCTCCTG CGTAGAGGAG GCTGGTTTTG CCCAGTGTGT CTGCCCAACT 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGTGGAT CAGATGGTGT CACATACGGC 
AATGAATGCC AGCTGAAGAC CATTGCCTGC CGCCAGCGTC TGGACATCTC CATTCAGAGT 
CTTGGTCCAT GCCGGGAGAG TGTTGCTCCT GGGGTTTCCC CTACATCTGC ATCTATGACC 
ACCCCAAGGC ATATCCTGAG CAGGACACTG GCGTCTCCCC ACAGCAGCCT TCCTCTGTCT 
CCCAGCACTA CTGCCCATGA TTGGCCCACC CCATTACCCA CATCACCTCA GACCGTAGTC 
GGCACCCCCA GGAGCACTGC AGCCACACCC TCTGATGTGG CCAGTCTTGC TACAGCGATC 
TTCAGGGAAT CTGGCAGCAC CAACGGCAGT GGCGATGAGG AGCTCAGTGG CGATGAGGAG 
GCCAGTGGGG GCGGGTCTGG GGGACTTGAG CCCCCGGTGG GCAGCGTTGT GGTGACCCAC 
GGGCCACCCA TCGAGAGGGC TTCCTGTTAC AACTCACCTT TGGGCTGCTG CTCAGATGGC 
AAGACACCCT CACTGGACTC AGAAGGCTCC AACTGTCCAG CTACCAAGGC ATTCCAGGGC 
GTGCTGGAGC TTGAGGGGGT CGAGGGACAG GAACTGTTCT ACACACCAGA GATGGCTGAC 
CCCAAGTCAG AGTTGTTTGG GGAGACTGCA AGGAGCATTG AGAGCACGCT GGACGACCTG 
TTCCGGAATT CGGATGTTAA GAAGGACTTC TGGAGCATCC GCCTACGGGA ACTGGGGCCT 
GGCAAATTAG TCCGTGCCAT TGTGGATGTT CACTTTGACC CCACCACAGC CTTCCAGGCA 
CCAGATGTGG GTCAGGCCTT GCTCCAACAG ATCCAGGTAT CCAGGCCGTG GGCCCTGGCA 
GTGAGGAGGC CTCTGCGGGA GCATGTGCGA TTCTTGGACT TTGACTGGTT TCCCACTTTT 
TTTACGGGAG CTGCAACAGG AACCACAGCT GCTGTGGCCA CAGCCAGAGC CACCACTGTG 
AGCCGACTGT CTGCCTCTTC TGTCACCCCA CGAGTCTACC CCAGTTACAC CAGCCGGCCT 
GTTGGCAGAA CTACGGCACC GCTAACCACT CGCCGGCCAC CAACCACTAC CGCCAGTATT 
GACCGACCTC GGACTCCAGG CCCGCAACGG CCCCCAAAGT CCTGTGATTC CCAGCCTTGC 
CTCCACGGAG GTACCTGCCA GGACCTGGAT TCTGGCAAGG GTTTCAGCTG CAGCTGTACT 
GCAGGCAGGG CTGGCACTGT CTGTGAGAAA GTGCAGCTCC CCTCTGTGCC AGCTTTTAAG 
GGCCACTCCT TCTTGGCCTT CCCCACCCTC CGAGCCTACC ACACGCTGCG TCTGGCACTA 
GAATTCCGGG CGCTGGAGAC AGAGGGACTG CTGCTCTACA ATGGCAATGC ACGTGGCAAA 
GATTTCCTGG CTCTGGCTCT GTTGGATGGT CATGTACAGT TCAGGTTCGA CACGGGCTCA 
GGGCCGGCGG TGCTAACAAG CTTAGTGCCA GTGGAACCGG GACGGTGGCA CCGCCTCGAG 
TTGTCACGGC ATTGGCGGCA GGGCACACTT TCTGTGGATG GCGAGGCTCC TGTTGTAGGT 
GAAAGTCCGA GTGGCACTGA TGGCCTCAAC TTGGACACGA AGCTCTATGT GGGTGGTCTC 
CCAGAAGAAC AAGTTGCCAC GGTGCTTGAT CGGACCTCTG TGGGCATCGG CCTGAAAGGA 
TGCATTCGTA TGTTGGACAT CAACAACCAG CAGCTGGAGC TGAGCGATTG GCAGAGGGCT 
GTGGTTCAAA GCTCTGGTGT GGGGGAATGC GGAGACCATC CCTGCTCACC TAACCCCTGC 
CATGGCGGGG CCCTCTGCCA GGCCCTGGAG GCTGGCGTGT TCCTCTGTCA GTGCCCACCT 
GGCCGCTTTG GCCCAACTTG TGCAGATGAA AAGAACCCCT GCCAACCGAA CCCCTGCCAC 
GGGTCAGCCC CCTGCCATGT GCTTTCCAGG GGTGGGGCCA AGTGTGCGTG CCCCCTGGGA 
CGCAGTGGTT CCTTCTGTGA GACAGTCCTG GAGAATGCTG GCTCCCGGCC CTTCCTGGCT 
GACTTTAATG GCTTCTCCTA CCTGGAACTG AAAGGCTTGC ACACCTTCGA GAGAGACCTA 
GGGGAGAAGA TGGCGCTGGA GATGGTGTTC TTGGCTCGTG GGCCCAGTGG CTTACTCCTC 
TACAATGGGC AGAAGACGGA TGGCAAGGGG GACTTTGTAT CCCTGGCCCT GCATAACCGG 
CACCTAGAGT TCCGCTATGA CCTTGGCAAG GGGGCTGCAA TCATCAGGAG CAAAGAGCCC 
ATAGCCCTGG GCACCTGGGT TAGGGTATTC CTGGAACGAA ATGGCCGCAA GGGTGCCCTT 
CAAGTGGGTG ATGGGCCCCG TGTGCTAGGG GAATCTCCGA AATCCCGCAA GGTCCCGCAC 
ACCATGCTCA ACCTCAAGGA GCCCCTCTAT GTGGGGGGAG CTCCTGACTT CAGCAAGCTG 
GCTCGGGGCG CTGCAGTGGC CTCCGGCTTT GATGGTGCCA TCCAGCTGGT GTCTCTAAGA 
GGCCATCAGC TGCTGACTCA GGAGCATGTG TTGCGGGCAG TAGATGTAGC GCCTTTTGCA 
GGCCACCCTT GTACCCAGGC CGTGGACAAC CCCTGCCTTA ATGGGGGCTC CTGTATCCCG 
AGGGAAGCCA CTTATGAGTG CCTGTGTCCT GGGGGCTTCT CTGGGCTGCA CTGCGAGAAG 
GGGATAGTTG AGAAGTCAGT GGGGGACCTA GAAACACTGG CCTTTGATGG GCGGACCTAC 
ATCGAGTACC TCAATGCTGT GACTGAGAGC GAGCTGACCA ATGAGATCCC AGCCCCCGAA 
ACTCTGGATT CCCGGGCCCT TTTCAGTGAG AAAGCGCTGC AGAGCAACCA CTTTGAGCTG 
AGCTTACGCA CTGAGGCCAC GCAGGGGCTG GTGCTGTGGA TTGGAAAGGT TGGAGAACGT 
GCAGACTACA TGGCTCTGGC CATTGTGGAT GGGCACCTAC AACTGAGCTA TGACCTAGGC 
TCCCAGCCAG TTGTGCTGCG CTCCACTGTG AAGGTCAACA CCAACCGCTG GCTTCGAGTC 
AGGGCTCACA GGGAGCACAG GGAAGGTTCC CTTCAGGTGG GCAATGAAGC CCCTGTGACT 
GGCTCTTCCC CGCTGGGTGC CACACAATTG GACACAGATG GAGCCCTGTG GCTTGGAGGC 
CTACAGAAGC TTCCTGTGGG GCAGGCTCTC CCCAAGGCCT ATGGCACGGG TTTTGTGGGC 
TGTCTGCGGG ACGTGGTAGT GGGCCATCGC CAGCTGCATC TGCTGGAGGA CGCTGTCACC 
AAACCAGAGC TAAGACCCTG CCCCACTCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355955.1
CDS	562..6414
Translation	MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	NM_001369026.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369026.1

ORF Insert Sequence:

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGCAGC CTGGTGCCTC CGTGCTGGTT 
CGGTACTTCA TGATCCCCTG CAACATCTGC TTGATCCTCT TGGCTACTTC TACGTTGGGC 
TTTGCGGTGC TGCTTTTCCT CAGCAACTAC AAACCTGGGA TCCACTTCAC AGCAGCGCCT 
TCTATGCCTC CTGATGTGTG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG GGCCTCCTGT GTGTGCAAGA AGAATGTCTG CCCTGCTATG 
GTAGCTCCTG TGTGTGGCTC AGATGCTTCC ACCTATAGCA ACGAGTGTGA GCTACAGCGT 
GCACAGTGCA ACCAGCAACG GCGCATCCGC CTACTCCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAATGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC AGCCGATGGA 
CAGACCGCCT CGTGTCTGTG TCCTACAACC TGCTTTGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGATG GTGTTGACTA CCCTAGTGAG TGCCAGCTGC TCCGTCATGC CTGTGCCAAC 
CAGGAGCACA TCTTTAAGAA GTTCGATGGT CCTTGTGACC CCTGCCAGGG CAGCATGTCA 
GACCTGAATC ATATTTGCCG GGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCCGCCCA ACACACACCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA GCCCGTGGCC TGCTTCTCCA GAAAGTGCGC 
TCTGGTCAAT GCCAGACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCTGTATGC 
CTGTCCCGCC GCGGCCGTCC CCACTGTTCC TGCGATCGCG TCACCTGTGA TGGGGCTTAC 
AGGCCAGTGT GTGCCCAGGA TGGGCACACG TATGACAATG ACTGTTGGCG CCAACAGGCC 
GAGTGTCGAC AACAGCAGAC CATTCCCCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCA 
TCCCCGTGCC GTGGAGCGCA GTGTGCATTT GGGGCAACAT GCACAGTGAA GAATGGGAAA 
GCTGTGTGCG AGTGCCAGCG GGTGTGCTCG GGCGGCTACG ATCCTGTGTG CGGCAGTGAT 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCGAGTGG CCCGCAGAGG ACCGTGTGAC CGATGTGGGC AGTGCCGGTT TGGATCCTTG 
TGCGAGGTGG AGACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGTGGCT CTGACGGACA CACATATGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACACACCAGA TCAGCCTATA CGTGGCCTCA GCCGGACACT GCCAGACCTG TGGAGAAACA 
GTTTGTACCT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCCCCACCTG GCCCTGTGTG CGGCAGTGAT GGCGTCACCT ACCTCAGTGC CTGTGAGCTC 
CGAGAGGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCGTGCAGG GCCGTGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGG TCTGGGGAAG ACAATGCGTG TGAGCAGGAG 
CTGTGTCGGC AGCATGGTGG TGTCTGGGAT GAGGACTCAG AAGACGGGCC GTGTGTCTGT 
GACTTTAGTT GCCAGAGTGT CCTTAAAAGC CCAGTGTGTG GCTCAGATGG AGTCACCTAT 
AGCACGGAGT GCCATCTGAA GAAGGCCAGA TGTGAAGCGC GGCAAGAGCT GTACGTCGCT 
GCTCAGGGAG CCTGCCGGGG CCCTACCTTG GCTCCACTGC TACCTATGGC CTCCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATGTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCACACG GCTCCTATAG CGGCACTTGT 
GACCCAGTCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GTGACCCTCG GGGTGCTGTA AGAGATGACT GTGAGCAGAT GACTGGATTG 
TGTTCCTGTA GACCTGGTGT GGCTGGTCCC AAGTGTGGGC AGTGTCCAGA TGGTCAAGCC 
CTGGGCCATC TTGGCTGTGA AGCAGATCCC ACAACACCAG TGACTTGTGT GGAAATGCAC 
TGTGAGTTTG GCGCCTCCTG CGTAGAGGAG GCTGGTTTTG CCCAGTGTGT CTGCCCAACT 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGTGGAT CAGATGGTGT CACATACGGC 
AATGAATGCC AGCTGAAGAC CATTGCCTGC CGCCAGCGTC TGGACATCTC CATTCAGAGT 
CTTGGTCCAT GCCGGGAGAG TGTTGCTCCT GGGGTTTCCC CTACATCTGC ATCTATGACC 
ACCCCAAGGC ATATCCTGAG CAGGACACTG GCGTCTCCCC ACAGCAGCCT TCCTCTGTCT 
CCCAGCACTA CTGCCCATGA TTGGCCCACC CCATTACCCA CATCACCTCA GACCGTAGTC 
GGCACCCCCA GGAGCACTGC AGCCACACCC TCTGATGTGG CCAGTCTTGC TACAGCGATC 
TTCAGGGAAT CTGGCAGCAC CAACGGCAGT GGCGATGAGG AGCTCAGTGG CGATGAGGAG 
GCCAGTGGGG GCGGGTCTGG GGGACTTGAG CCCCCGGTGG GCAGCGTTGT GGTGACCCAC 
GGGCCACCCA TCGAGAGGGC TTCCTGTTAC AACTCACCTT TGGGCTGCTG CTCAGATGGC 
AAGACACCCT CACTGGACTC AGAAGGCTCC AACTGTCCAG CTACCAAGGC ATTCCAGGGC 
GTGCTGGAGC TTGAGGGGGT CGAGGGACAG GAACTGTTCT ACACACCAGA GATGGCTGAC 
CCCAAGTCAG AGTTGTTTGG GGAGACTGCA AGGAGCATTG AGAGCACGCT GGACGACCTG 
TTCCGGAATT CGGATGTTAA GAAGGACTTC TGGAGCATCC GCCTACGGGA ACTGGGGCCT 
GGCAAATTAG TCCGTGCCAT TGTGGATGTT CACTTTGACC CCACCACAGC CTTCCAGGCA 
CCAGATGTGG GTCAGGCCTT GCTCCAACAG ATCCAGGTAT CCAGGCCGTG GGCCCTGGCA 
GTGAGGAGGC CTCTGCGGGA GCATGTGCGA TTCTTGGACT TTGACTGGTT TCCCACTTTT 
TTTACGGGAG CTGCAACAGG AACCACAGCT GCTGTGGCCA CAGCCAGAGC CACCACTGTG 
AGCCGACTGT CTGCCTCTTC TGTCACCCCA CGAGTCTACC CCAGTTACAC CAGCCGGCCT 
GTTGGCAGAA CTACGGCACC GCTAACCACT CGCCGGCCAC CAACCACTAC CGCCAGTATT 
GACCGACCTC GGACTCCAGG CCCGCAACGG CCCCCAAAGT CCTGTGATTC CCAGCCTTGC 
CTCCACGGAG GTACCTGCCA GGACCTGGAT TCTGGCAAGG GTTTCAGCTG CAGCTGTACT 
GCAGGCAGGG CTGGCACTGT CTGTGAGAAA GTGCAGCTCC CCTCTGTGCC AGCTTTTAAG 
GGCCACTCCT TCTTGGCCTT CCCCACCCTC CGAGCCTACC ACACGCTGCG TCTGGCACTA 
GAATTCCGGG CGCTGGAGAC AGAGGGACTG CTGCTCTACA ATGGCAATGC ACGTGGCAAA 
GATTTCCTGG CTCTGGCTCT GTTGGATGGT CATGTACAGT TCAGGTTCGA CACGGGCTCA 
GGGCCGGCGG TGCTAACAAG CTTAGTGCCA GTGGAACCGG GACGGTGGCA CCGCCTCGAG 
TTGTCACGGC ATTGGCGGCA GGGCACACTT TCTGTGGATG GCGAGGCTCC TGTTGTAGGT 
GAAAGTCCGA GTGGCACTGA TGGCCTCAAC TTGGACACGA AGCTCTATGT GGGTGGTCTC 
CCAGAAGAAC AAGTTGCCAC GGTGCTTGAT CGGACCTCTG TGGGCATCGG CCTGAAAGGA 
TGCATTCGTA TGTTGGACAT CAACAACCAG CAGCTGGAGC TGAGCGATTG GCAGAGGGCT 
GTGGTTCAAA GCTCTGGTGT GGGGGAATGC GGAGACCATC CCTGCTCACC TAACCCCTGC 
CATGGCGGGG CCCTCTGCCA GGCCCTGGAG GCTGGCGTGT TCCTCTGTCA GTGCCCACCT 
GGCCGCTTTG GCCCAACTTG TGCAGATGAA AAGAACCCCT GCCAACCGAA CCCCTGCCAC 
GGGTCAGCCC CCTGCCATGT GCTTTCCAGG GGTGGGGCCA AGTGTGCGTG CCCCCTGGGA 
CGCAGTGGTT CCTTCTGTGA GACAGTCCTG GAGAATGCTG GCTCCCGGCC CTTCCTGGCT 
GACTTTAATG GCTTCTCCTA CCTGGAACTG AAAGGCTTGC ACACCTTCGA GAGAGACCTA 
GGGGAGAAGA TGGCGCTGGA GATGGTGTTC TTGGCTCGTG GGCCCAGTGG CTTACTCCTC 
TACAATGGGC AGAAGACGGA TGGCAAGGGG GACTTTGTAT CCCTGGCCCT GCATAACCGG 
CACCTAGAGT TCCGCTATGA CCTTGGCAAG GGGGCTGCAA TCATCAGGAG CAAAGAGCCC 
ATAGCCCTGG GCACCTGGGT TAGGGTATTC CTGGAACGAA ATGGCCGCAA GGGTGCCCTT 
CAAGTGGGTG ATGGGCCCCG TGTGCTAGGG GAATCTCCGA AATCCCGCAA GGTCCCGCAC 
ACCATGCTCA ACCTCAAGGA GCCCCTCTAT GTGGGGGGAG CTCCTGACTT CAGCAAGCTG 
GCTCGGGGCG CTGCAGTGGC CTCCGGCTTT GATGGTGCCA TCCAGCTGGT GTCTCTAAGA 
GGCCATCAGC TGCTGACTCA GGAGCATGTG TTGCGGGCAG TAGATGTAGC GCCTTTTGCA 
GGCCACCCTT GTACCCAGGC CGTGGACAAC CCCTGCCTTA ATGGGGGCTC CTGTATCCCG 
AGGGAAGCCA CTTATGAGTG CCTGTGTCCT GGGGGCTTCT CTGGGCTGCA CTGCGAGAAG 
GGGATAGTTG AGAAGTCAGT GGGGGACCTA GAAACACTGG CCTTTGATGG GCGGACCTAC 
ATCGAGTACC TCAATGCTGT GACTGAGAGC GAGCTGACCA ATGAGATCCC AGCCCCCGAA 
ACTCTGGATT CCCGGGCCCT TTTCAGTGAG AAAGCGCTGC AGAGCAACCA CTTTGAGCTG 
AGCTTACGCA CTGAGGCCAC GCAGGGGCTG GTGCTGTGGA TTGGAAAGGT TGGAGAACGT 
GCAGACTACA TGGCTCTGGC CATTGTGGAT GGGCACCTAC AACTGAGCTA TGACCTAGGC 
TCCCAGCCAG TTGTGCTGCG CTCCACTGTG AAGGTCAACA CCAACCGCTG GCTTCGAGTC 
AGGGCTCACA GGGAGCACAG GGAAGGTTCC CTTCAGGTGG GCAATGAAGC CCCTGTGACT 
GGCTCTTCCC CGCTGGGTGC CACACAATTG GACACAGATG GAGCCCTGTG GCTTGGAGGC 
CTACAGAAGC TTCCTGTGGG GCAGGCTCTC CCCAAGGCCT ATGGCACGGG TTTTGTGGGC 
TGTCTGCGGG ACGTGGTAGT GGGCCATCGC CAGCTGCATC TGCTGGAGGA CGCTGTCACC 
AAACCAGAGC TAAGACCCTG CCCCACTCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538491.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538554.1 Mus musculus agrin (Agrn), transcript variant X1, mRNA.

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CloneID	OMu33953
Clone ID Related Accession (Same CDS sequence)	XM_006538491.1 , XM_006538491.2
Accession Version	XM_006538491.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CGAGCTGACC 
AATGAGATCC CAGCCCCCGA AACTCTGGAT TCCCGGGCCC TTTTCAGTGA GAAAGCGCTG 
CAGAGCAACC ACTTTGAGCT GAGCTTACGC ACTGAGGCCA CGCAGGGGCT GGTGCTGTGG 
ATTGGAAAGG TTGGAGAACG TGCAGACTAC ATGGCTCTGG CCATTGTGGA TGGGCACCTA 
CAACTGAGCT ATGACCTAGG CTCCCAGCCA GTTGTGCTGC GCTCCACTGT GAAGGTCAAC 
ACCAACCGCT GGCTTCGAGT CAGGGCTCAC AGGGAGCACA GGGAAGGTTC CCTTCAGGTG 
GGCAATGAAG CCCCTGTGAC TGGCTCTTCC CCGCTGGGTG CCACACAATT GGACACAGAT 
GGAGCCCTGT GGCTTGGAGG CCTACAGAAG CTTCCTGTGG GGCAGGCTCT CCCCAAGGCC 
TATGGCACGG GTTTTGTGGG CTGTCTGCGG GACGTGGTAG TGGGCCATCG CCAGCTGCAT 
CTGCTGGAGG ACGCTGTCAC CAAACCAGAG CTAAGACCCT GCCCCACTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538554.1
CDS	99..6272
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538491.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538491.1

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CGAGCTGACC 
AATGAGATCC CAGCCCCCGA AACTCTGGAT TCCCGGGCCC TTTTCAGTGA GAAAGCGCTG 
CAGAGCAACC ACTTTGAGCT GAGCTTACGC ACTGAGGCCA CGCAGGGGCT GGTGCTGTGG 
ATTGGAAAGG TTGGAGAACG TGCAGACTAC ATGGCTCTGG CCATTGTGGA TGGGCACCTA 
CAACTGAGCT ATGACCTAGG CTCCCAGCCA GTTGTGCTGC GCTCCACTGT GAAGGTCAAC 
ACCAACCGCT GGCTTCGAGT CAGGGCTCAC AGGGAGCACA GGGAAGGTTC CCTTCAGGTG 
GGCAATGAAG CCCCTGTGAC TGGCTCTTCC CCGCTGGGTG CCACACAATT GGACACAGAT 
GGAGCCCTGT GGCTTGGAGG CCTACAGAAG CTTCCTGTGG GGCAGGCTCT CCCCAAGGCC 
TATGGCACGG GTTTTGTGGG CTGTCTGCGG GACGTGGTAG TGGGCCATCG CCAGCTGCAT 
CTGCTGGAGG ACGCTGTCAC CAAACCAGAG CTAAGACCCT GCCCCACTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538492.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538555.1 Mus musculus agrin (Agrn), transcript variant X3, mRNA.

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CloneID	OMu33954
Clone ID Related Accession (Same CDS sequence)	XM_006538492.2 , XM_006538492.1
Accession Version	XM_006538492.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CCCCGAAACT 
CTGGATTCCC GGGCCCTTTT CAGTGAGAAA GCGCTGCAGA GCAACCACTT TGAGCTGAGC 
TTACGCACTG AGGCCACGCA GGGGCTGGTG CTGTGGATTG GAAAGGTTGG AGAACGTGCA 
GACTACATGG CTCTGGCCAT TGTGGATGGG CACCTACAAC TGAGCTATGA CCTAGGCTCC 
CAGCCAGTTG TGCTGCGCTC CACTGTGAAG GTCAACACCA ACCGCTGGCT TCGAGTCAGG 
GCTCACAGGG AGCACAGGGA AGGTTCCCTT CAGGTGGGCA ATGAAGCCCC TGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAATTGGAC ACAGATGGAG CCCTGTGGCT TGGAGGCCTA 
CAGAAGCTTC CTGTGGGGCA GGCTCTCCCC AAGGCCTATG GCACGGGTTT TGTGGGCTGT 
CTGCGGGACG TGGTAGTGGG CCATCGCCAG CTGCATCTGC TGGAGGACGC TGTCACCAAA 
CCAGAGCTAA GACCCTGCCC CACTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538555.1
CDS	99..6248
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538492.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538492.1

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CCCCGAAACT 
CTGGATTCCC GGGCCCTTTT CAGTGAGAAA GCGCTGCAGA GCAACCACTT TGAGCTGAGC 
TTACGCACTG AGGCCACGCA GGGGCTGGTG CTGTGGATTG GAAAGGTTGG AGAACGTGCA 
GACTACATGG CTCTGGCCAT TGTGGATGGG CACCTACAAC TGAGCTATGA CCTAGGCTCC 
CAGCCAGTTG TGCTGCGCTC CACTGTGAAG GTCAACACCA ACCGCTGGCT TCGAGTCAGG 
GCTCACAGGG AGCACAGGGA AGGTTCCCTT CAGGTGGGCA ATGAAGCCCC TGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAATTGGAC ACAGATGGAG CCCTGTGGCT TGGAGGCCTA 
CAGAAGCTTC CTGTGGGGCA GGCTCTCCCC AAGGCCTATG GCACGGGTTT TGTGGGCTGT 
CTGCGGGACG TGGTAGTGGG CCATCGCCAG CTGCATCTGC TGGAGGACGC TGTCACCAAA 
CCAGAGCTAA GACCCTGCCC CACTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538493.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538556.1 Mus musculus agrin (Agrn), transcript variant X4, mRNA.

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CloneID	OMu33955
Clone ID Related Accession (Same CDS sequence)	XM_006538493.1 , XM_006538493.2
Accession Version	XM_006538493.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CGAGCTGACC 
AATGAGATCC CAGCTGAGAA AGCGCTGCAG AGCAACCACT TTGAGCTGAG CTTACGCACT 
GAGGCCACGC AGGGGCTGGT GCTGTGGATT GGAAAGGTTG GAGAACGTGC AGACTACATG 
GCTCTGGCCA TTGTGGATGG GCACCTACAA CTGAGCTATG ACCTAGGCTC CCAGCCAGTT 
GTGCTGCGCT CCACTGTGAA GGTCAACACC AACCGCTGGC TTCGAGTCAG GGCTCACAGG 
GAGCACAGGG AAGGTTCCCT TCAGGTGGGC AATGAAGCCC CTGTGACTGG CTCTTCCCCG 
CTGGGTGCCA CACAATTGGA CACAGATGGA GCCCTGTGGC TTGGAGGCCT ACAGAAGCTT 
CCTGTGGGGC AGGCTCTCCC CAAGGCCTAT GGCACGGGTT TTGTGGGCTG TCTGCGGGAC 
GTGGTAGTGG GCCATCGCCA GCTGCATCTG CTGGAGGACG CTGTCACCAA ACCAGAGCTA 
AGACCCTGCC CCACTCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538556.1
CDS	99..6239
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538493.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538493.1

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG CGAGCTGACC 
AATGAGATCC CAGCTGAGAA AGCGCTGCAG AGCAACCACT TTGAGCTGAG CTTACGCACT 
GAGGCCACGC AGGGGCTGGT GCTGTGGATT GGAAAGGTTG GAGAACGTGC AGACTACATG 
GCTCTGGCCA TTGTGGATGG GCACCTACAA CTGAGCTATG ACCTAGGCTC CCAGCCAGTT 
GTGCTGCGCT CCACTGTGAA GGTCAACACC AACCGCTGGC TTCGAGTCAG GGCTCACAGG 
GAGCACAGGG AAGGTTCCCT TCAGGTGGGC AATGAAGCCC CTGTGACTGG CTCTTCCCCG 
CTGGGTGCCA CACAATTGGA CACAGATGGA GCCCTGTGGC TTGGAGGCCT ACAGAAGCTT 
CCTGTGGGGC AGGCTCTCCC CAAGGCCTAT GGCACGGGTT TTGTGGGCTG TCTGCGGGAC 
GTGGTAGTGG GCCATCGCCA GCTGCATCTG CTGGAGGACG CTGTCACCAA ACCAGAGCTA 
AGACCCTGCC CCACTCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538494.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538557.1 Mus musculus agrin (Agrn), transcript variant X5, mRNA.

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CloneID	OMu33956
Clone ID Related Accession (Same CDS sequence)	XM_006538494.3 , XM_006538494.4
Accession Version	XM_006538494.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG TGAGAAAGCG 
CTGCAGAGCA ACCACTTTGA GCTGAGCTTA CGCACTGAGG CCACGCAGGG GCTGGTGCTG 
TGGATTGGAA AGGTTGGAGA ACGTGCAGAC TACATGGCTC TGGCCATTGT GGATGGGCAC 
CTACAACTGA GCTATGACCT AGGCTCCCAG CCAGTTGTGC TGCGCTCCAC TGTGAAGGTC 
AACACCAACC GCTGGCTTCG AGTCAGGGCT CACAGGGAGC ACAGGGAAGG TTCCCTTCAG 
GTGGGCAATG AAGCCCCTGT GACTGGCTCT TCCCCGCTGG GTGCCACACA ATTGGACACA 
GATGGAGCCC TGTGGCTTGG AGGCCTACAG AAGCTTCCTG TGGGGCAGGC TCTCCCCAAG 
GCCTATGGCA CGGGTTTTGT GGGCTGTCTG CGGGACGTGG TAGTGGGCCA TCGCCAGCTG 
CATCTGCTGG AGGACGCTGT CACCAAACCA GAGCTAAGAC CCTGCCCCAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538557.1
CDS	98..6214
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538494.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538494.3

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
AAATCCCGCA AGGTCCCGCA CACCATGCTC AACCTCAAGG AGCCCCTCTA TGTGGGGGGA 
GCTCCTGACT TCAGCAAGCT GGCTCGGGGC GCTGCAGTGG CCTCCGGCTT TGATGGTGCC 
ATCCAGCTGG TGTCTCTAAG AGGCCATCAG CTGCTGACTC AGGAGCATGT GTTGCGGGCA 
GTAGATGTAG CGCCTTTTGC AGGCCACCCT TGTACCCAGG CCGTGGACAA CCCCTGCCTT 
AATGGGGGCT CCTGTATCCC GAGGGAAGCC ACTTATGAGT GCCTGTGTCC TGGGGGCTTC 
TCTGGGCTGC ACTGCGAGAA GGGGATAGTT GAGAAGTCAG TGGGGGACCT AGAAACACTG 
GCCTTTGATG GGCGGACCTA CATCGAGTAC CTCAATGCTG TGACTGAGAG TGAGAAAGCG 
CTGCAGAGCA ACCACTTTGA GCTGAGCTTA CGCACTGAGG CCACGCAGGG GCTGGTGCTG 
TGGATTGGAA AGGTTGGAGA ACGTGCAGAC TACATGGCTC TGGCCATTGT GGATGGGCAC 
CTACAACTGA GCTATGACCT AGGCTCCCAG CCAGTTGTGC TGCGCTCCAC TGTGAAGGTC 
AACACCAACC GCTGGCTTCG AGTCAGGGCT CACAGGGAGC ACAGGGAAGG TTCCCTTCAG 
GTGGGCAATG AAGCCCCTGT GACTGGCTCT TCCCCGCTGG GTGCCACACA ATTGGACACA 
GATGGAGCCC TGTGGCTTGG AGGCCTACAG AAGCTTCCTG TGGGGCAGGC TCTCCCCAAG 
GCCTATGGCA CGGGTTTTGT GGGCTGTCTG CGGGACGTGG TAGTGGGCCA TCGCCAGCTG 
CATCTGCTGG AGGACGCTGT CACCAAACCA GAGCTAAGAC CCTGCCCCAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538495.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538558.1 Mus musculus agrin (Agrn), transcript variant X7, mRNA.

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CloneID	OMu33957
Clone ID Related Accession (Same CDS sequence)	XM_006538495.2 , XM_006538495.3
Accession Version	XM_006538495.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	agrin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGCAGC CTGGTGCCTC CGTGCTGGTT 
CGGTACTTCA TGATCCCCTG CAACATCTGC TTGATCCTCT TGGCTACTTC TACGTTGGGC 
TTTGCGGTGC TGCTTTTCCT CAGCAACTAC AAACCTGGGA TCCACTTCAC AGCAGCGCCT 
TCTATGCCTC CTGATGTGTG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG GGCCTCCTGT GTGTGCAAGA AGAATGTCTG CCCTGCTATG 
GTAGCTCCTG TGTGTGGCTC AGATGCTTCC ACCTATAGCA ACGAGTGTGA GCTACAGCGT 
GCACAGTGCA ACCAGCAACG GCGCATCCGC CTACTCCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAATGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC AGCCGATGGA 
CAGACCGCCT CGTGTCTGTG TCCTACAACC TGCTTTGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGATG GTGTTGACTA CCCTAGTGAG TGCCAGCTGC TCCGTCATGC CTGTGCCAAC 
CAGGAGCACA TCTTTAAGAA GTTCGATGGT CCTTGTGACC CCTGCCAGGG CAGCATGTCA 
GACCTGAATC ATATTTGCCG GGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCCGCCCA ACACACACCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA GCCCGTGGCC TGCTTCTCCA GAAAGTGCGC 
TCTGGTCAAT GCCAGACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCTGTATGC 
CTGTCCCGCC GCGGCCGTCC CCACTGTTCC TGCGATCGCG TCACCTGTGA TGGGGCTTAC 
AGGCCAGTGT GTGCCCAGGA TGGGCACACG TATGACAATG ACTGTTGGCG CCAACAGGCC 
GAGTGTCGAC AACAGCAGAC CATTCCCCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCA 
TCCCCGTGCC GTGGAGCGCA GTGTGCATTT GGGGCAACAT GCACAGTGAA GAATGGGAAA 
GCTGTGTGCG AGTGCCAGCG GGTGTGCTCG GGCGGCTACG ATCCTGTGTG CGGCAGTGAT 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCGAGTGG CCCGCAGAGG ACCGTGTGAC CGATGTGGGC AGTGCCGGTT TGGATCCTTG 
TGCGAGGTGG AGACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGTGGCT CTGACGGACA CACATATGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACACACCAGA TCAGCCTATA CGTGGCCTCA GCCGGACACT GCCAGACCTG TGGAGAAACA 
GTTTGTACCT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCCCCACCTG GCCCTGTGTG CGGCAGTGAT GGCGTCACCT ACCTCAGTGC CTGTGAGCTC 
CGAGAGGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCGTGCAGG GCCGTGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGG TCTGGGGAAG ACAATGCGTG TGAGCAGGAG 
CTGTGTCGGC AGCATGGTGG TGTCTGGGAT GAGGACTCAG AAGACGGGCC GTGTGTCTGT 
GACTTTAGTT GCCAGAGTGT CCTTAAAAGC CCAGTGTGTG GCTCAGATGG AGTCACCTAT 
AGCACGGAGT GCCATCTGAA GAAGGCCAGA TGTGAAGCGC GGCAAGAGCT GTACGTCGCT 
GCTCAGGGAG CCTGCCGGGG CCCTACCTTG GCTCCACTGC TACCTATGGC CTCCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATGTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCACACG GCTCCTATAG CGGCACTTGT 
GACCCAGTCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GTGACCCTCG GGGTGCTGTA AGAGATGACT GTGAGCAGAT GACTGGATTG 
TGTTCCTGTA GACCTGGTGT GGCTGGTCCC AAGTGTGGGC AGTGTCCAGA TGGTCAAGCC 
CTGGGCCATC TTGGCTGTGA AGCAGATCCC ACAACACCAG TGACTTGTGT GGAAATGCAC 
TGTGAGTTTG GCGCCTCCTG CGTAGAGGAG GCTGGTTTTG CCCAGTGTGT CTGCCCAACT 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGTGGAT CAGATGGTGT CACATACGGC 
AATGAATGCC AGCTGAAGAC CATTGCCTGC CGCCAGCGTC TGGACATCTC CATTCAGAGT 
CTTGGTCCAT GCCGGGAGAG TGTTGCTCCT GGGGTTTCCC CTACATCTGC ATCTATGACC 
ACCCCAAGGC ATATCCTGAG CAGGACACTG GCGTCTCCCC ACAGCAGCCT TCCTCTGTCT 
CCCAGCACTA CTGCCCATGA TTGGCCCACC CCATTACCCA CATCACCTCA GACCGTAGTC 
GGCACCCCCA GGAGCACTGC AGCCACACCC TCTGATGTGG CCAGTCTTGC TACAGCGATC 
TTCAGGGAAT CTGGCAGCAC CAACGGCAGT GGCGATGAGG AGCTCAGTGG CGATGAGGAG 
GCCAGTGGGG GCGGGTCTGG GGGACTTGAG CCCCCGGTGG GCAGCGTTGT GGTGACCCAC 
GGGCCACCCA TCGAGAGGGC TTCCTGTTAC AACTCACCTT TGGGCTGCTG CTCAGATGGC 
AAGACACCCT CACTGGACTC AGAAGGCTCC AACTGTCCAG CTACCAAGGC ATTCCAGGGC 
GTGCTGGAGC TTGAGGGGGT CGAGGGACAG GAACTGTTCT ACACACCAGA GATGGCTGAC 
CCCAAGTCAG AGTTGTTTGG GGAGACTGCA AGGAGCATTG AGAGCACGCT GGACGACCTG 
TTCCGGAATT CGGATGTTAA GAAGGACTTC TGGAGCATCC GCCTACGGGA ACTGGGGCCT 
GGCAAATTAG TCCGTGCCAT TGTGGATGTT CACTTTGACC CCACCACAGC CTTCCAGGCA 
CCAGATGTGG GTCAGGCCTT GCTCCAACAG ATCCAGGTAT CCAGGCCGTG GGCCCTGGCA 
GTGAGGAGGC CTCTGCGGGA GCATGTGCGA TTCTTGGACT TTGACTGGTT TCCCACTTTT 
TTTACGGGAG CTGCAACAGG AACCACAGCT GCTGTGGCCA CAGCCAGAGC CACCACTGTG 
AGCCGACTGT CTGCCTCTTC TGTCACCCCA CGAGTCTACC CCAGTTACAC CAGCCGGCCT 
GTTGGCAGAA CTACGGCACC GCTAACCACT CGCCGGCCAC CAACCACTAC CGCCAGTATT 
GACCGACCTC GGACTCCAGG CCCGCAACGG CCCCCAAAGT CCTGTGATTC CCAGCCTTGC 
CTCCACGGAG GTACCTGCCA GGACCTGGAT TCTGGCAAGG GTTTCAGCTG CAGCTGTACT 
GCAGGCAGGG CTGGCACTGT CTGTGAGAAA GTGCAGCTCC CCTCTGTGCC AGCTTTTAAG 
GGCCACTCCT TCTTGGCCTT CCCCACCCTC CGAGCCTACC ACACGCTGCG TCTGGCACTA 
GAATTCCGGG CGCTGGAGAC AGAGGGACTG CTGCTCTACA ATGGCAATGC ACGTGGCAAA 
GATTTCCTGG CTCTGGCTCT GTTGGATGGT CATGTACAGT TCAGGTTCGA CACGGGCTCA 
GGGCCGGCGG TGCTAACAAG CTTAGTGCCA GTGGAACCGG GACGGTGGCA CCGCCTCGAG 
TTGTCACGGC ATTGGCGGCA GGGCACACTT TCTGTGGATG GCGAGGCTCC TGTTGTAGGT 
GAAAGTCCGA GTGGCACTGA TGGCCTCAAC TTGGACACGA AGCTCTATGT GGGTGGTCTC 
CCAGAAGAAC AAGTTGCCAC GGTGCTTGAT CGGACCTCTG TGGGCATCGG CCTGAAAGGA 
TGCATTCGTA TGTTGGACAT CAACAACCAG CAGCTGGAGC TGAGCGATTG GCAGAGGGCT 
GTGGTTCAAA GCTCTGGTGT GGGGGAATGC GGAGACCATC CCTGCTCACC TAACCCCTGC 
CATGGCGGGG CCCTCTGCCA GGCCCTGGAG GCTGGCGTGT TCCTCTGTCA GTGCCCACCT 
GGCCGCTTTG GCCCAACTTG TGCAGATGAA AAGAACCCCT GCCAACCGAA CCCCTGCCAC 
GGGTCAGCCC CCTGCCATGT GCTTTCCAGG GGTGGGGCCA AGTGTGCGTG CCCCCTGGGA 
CGCAGTGGTT CCTTCTGTGA GACAGTCCTG GAGAATGCTG GCTCCCGGCC CTTCCTGGCT 
GACTTTAATG GCTTCTCCTA CCTGGAACTG AAAGGCTTGC ACACCTTCGA GAGAGACCTA 
GGGGAGAAGA TGGCGCTGGA GATGGTGTTC TTGGCTCGTG GGCCCAGTGG CTTACTCCTC 
TACAATGGGC AGAAGACGGA TGGCAAGGGG GACTTTGTAT CCCTGGCCCT GCATAACCGG 
CACCTAGAGT TCCGCTATGA CCTTGGCAAG GGGGCTGCAA TCATCAGGAG CAAAGAGCCC 
ATAGCCCTGG GCACCTGGGT TAGGGTATTC CTGGAACGAA ATGGCCGCAA GGGTGCCCTT 
CAAGTGGGTG ATGGGCCCCG TGTGCTAGGG GAATCTCCGA AATCCCGCAA GGTCCCGCAC 
ACCATGCTCA ACCTCAAGGA GCCCCTCTAT GTGGGGGGAG CTCCTGACTT CAGCAAGCTG 
GCTCGGGGCG CTGCAGTGGC CTCCGGCTTT GATGGTGCCA TCCAGCTGGT GTCTCTAAGA 
GGCCATCAGC TGCTGACTCA GGAGCATGTG TTGCGGGCAG TAGATGTAGC GCCTTTTGCA 
GGCCACCCTT GTACCCAGGC CGTGGACAAC CCCTGCCTTA ATGGGGGCTC CTGTATCCCG 
AGGGAAGCCA CTTATGAGTG CCTGTGTCCT GGGGGCTTCT CTGGGCTGCA CTGCGAGAAG 
GGGATAGTTG AGAAGTCAGT GGGGGACCTA GAAACACTGG CCTTTGATGG GCGGACCTAC 
ATCGAGTACC TCAATGCTGT GACTGAGAGT GAGAAAGCGC TGCAGAGCAA CCACTTTGAG 
CTGAGCTTAC GCACTGAGGC CACGCAGGGG CTGGTGCTGT GGATTGGAAA GGTTGGAGAA 
CGTGCAGACT ACATGGCTCT GGCCATTGTG GATGGGCACC TACAACTGAG CTATGACCTA 
GGCTCCCAGC CAGTTGTGCT GCGCTCCACT GTGAAGGTCA ACACCAACCG CTGGCTTCGA 
GTCAGGGCTC ACAGGGAGCA CAGGGAAGGT TCCCTTCAGG TGGGCAATGA AGCCCCTGTG 
ACTGGCTCTT CCCCGCTGGG TGCCACACAA TTGGACACAG ATGGAGCCCT GTGGCTTGGA 
GGCCTACAGA AGCTTCCTGT GGGGCAGGCT CTCCCCAAGG CCTATGGCAC GGGTTTTGTG 
GGCTGTCTGC GGGACGTGGT AGTGGGCCAT CGCCAGCTGC ATCTGCTGGA GGACGCTGTC 
ACCAAACCAG AGCTAAGACC CTGCCCCACT CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538558.1
CDS	535..6330
Translation	MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538495.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538495.2

ORF Insert Sequence:

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGCAGC CTGGTGCCTC CGTGCTGGTT 
CGGTACTTCA TGATCCCCTG CAACATCTGC TTGATCCTCT TGGCTACTTC TACGTTGGGC 
TTTGCGGTGC TGCTTTTCCT CAGCAACTAC AAACCTGGGA TCCACTTCAC AGCAGCGCCT 
TCTATGCCTC CTGATGTGTG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG GGCCTCCTGT GTGTGCAAGA AGAATGTCTG CCCTGCTATG 
GTAGCTCCTG TGTGTGGCTC AGATGCTTCC ACCTATAGCA ACGAGTGTGA GCTACAGCGT 
GCACAGTGCA ACCAGCAACG GCGCATCCGC CTACTCCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAATGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC AGCCGATGGA 
CAGACCGCCT CGTGTCTGTG TCCTACAACC TGCTTTGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGATG GTGTTGACTA CCCTAGTGAG TGCCAGCTGC TCCGTCATGC CTGTGCCAAC 
CAGGAGCACA TCTTTAAGAA GTTCGATGGT CCTTGTGACC CCTGCCAGGG CAGCATGTCA 
GACCTGAATC ATATTTGCCG GGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCCGCCCA ACACACACCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA GCCCGTGGCC TGCTTCTCCA GAAAGTGCGC 
TCTGGTCAAT GCCAGACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCTGTATGC 
CTGTCCCGCC GCGGCCGTCC CCACTGTTCC TGCGATCGCG TCACCTGTGA TGGGGCTTAC 
AGGCCAGTGT GTGCCCAGGA TGGGCACACG TATGACAATG ACTGTTGGCG CCAACAGGCC 
GAGTGTCGAC AACAGCAGAC CATTCCCCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCA 
TCCCCGTGCC GTGGAGCGCA GTGTGCATTT GGGGCAACAT GCACAGTGAA GAATGGGAAA 
GCTGTGTGCG AGTGCCAGCG GGTGTGCTCG GGCGGCTACG ATCCTGTGTG CGGCAGTGAT 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCGAGTGG CCCGCAGAGG ACCGTGTGAC CGATGTGGGC AGTGCCGGTT TGGATCCTTG 
TGCGAGGTGG AGACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGTGGCT CTGACGGACA CACATATGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACACACCAGA TCAGCCTATA CGTGGCCTCA GCCGGACACT GCCAGACCTG TGGAGAAACA 
GTTTGTACCT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCCCCACCTG GCCCTGTGTG CGGCAGTGAT GGCGTCACCT ACCTCAGTGC CTGTGAGCTC 
CGAGAGGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCGTGCAGG GCCGTGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGG TCTGGGGAAG ACAATGCGTG TGAGCAGGAG 
CTGTGTCGGC AGCATGGTGG TGTCTGGGAT GAGGACTCAG AAGACGGGCC GTGTGTCTGT 
GACTTTAGTT GCCAGAGTGT CCTTAAAAGC CCAGTGTGTG GCTCAGATGG AGTCACCTAT 
AGCACGGAGT GCCATCTGAA GAAGGCCAGA TGTGAAGCGC GGCAAGAGCT GTACGTCGCT 
GCTCAGGGAG CCTGCCGGGG CCCTACCTTG GCTCCACTGC TACCTATGGC CTCCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATGTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCACACG GCTCCTATAG CGGCACTTGT 
GACCCAGTCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GTGACCCTCG GGGTGCTGTA AGAGATGACT GTGAGCAGAT GACTGGATTG 
TGTTCCTGTA GACCTGGTGT GGCTGGTCCC AAGTGTGGGC AGTGTCCAGA TGGTCAAGCC 
CTGGGCCATC TTGGCTGTGA AGCAGATCCC ACAACACCAG TGACTTGTGT GGAAATGCAC 
TGTGAGTTTG GCGCCTCCTG CGTAGAGGAG GCTGGTTTTG CCCAGTGTGT CTGCCCAACT 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGTGGAT CAGATGGTGT CACATACGGC 
AATGAATGCC AGCTGAAGAC CATTGCCTGC CGCCAGCGTC TGGACATCTC CATTCAGAGT 
CTTGGTCCAT GCCGGGAGAG TGTTGCTCCT GGGGTTTCCC CTACATCTGC ATCTATGACC 
ACCCCAAGGC ATATCCTGAG CAGGACACTG GCGTCTCCCC ACAGCAGCCT TCCTCTGTCT 
CCCAGCACTA CTGCCCATGA TTGGCCCACC CCATTACCCA CATCACCTCA GACCGTAGTC 
GGCACCCCCA GGAGCACTGC AGCCACACCC TCTGATGTGG CCAGTCTTGC TACAGCGATC 
TTCAGGGAAT CTGGCAGCAC CAACGGCAGT GGCGATGAGG AGCTCAGTGG CGATGAGGAG 
GCCAGTGGGG GCGGGTCTGG GGGACTTGAG CCCCCGGTGG GCAGCGTTGT GGTGACCCAC 
GGGCCACCCA TCGAGAGGGC TTCCTGTTAC AACTCACCTT TGGGCTGCTG CTCAGATGGC 
AAGACACCCT CACTGGACTC AGAAGGCTCC AACTGTCCAG CTACCAAGGC ATTCCAGGGC 
GTGCTGGAGC TTGAGGGGGT CGAGGGACAG GAACTGTTCT ACACACCAGA GATGGCTGAC 
CCCAAGTCAG AGTTGTTTGG GGAGACTGCA AGGAGCATTG AGAGCACGCT GGACGACCTG 
TTCCGGAATT CGGATGTTAA GAAGGACTTC TGGAGCATCC GCCTACGGGA ACTGGGGCCT 
GGCAAATTAG TCCGTGCCAT TGTGGATGTT CACTTTGACC CCACCACAGC CTTCCAGGCA 
CCAGATGTGG GTCAGGCCTT GCTCCAACAG ATCCAGGTAT CCAGGCCGTG GGCCCTGGCA 
GTGAGGAGGC CTCTGCGGGA GCATGTGCGA TTCTTGGACT TTGACTGGTT TCCCACTTTT 
TTTACGGGAG CTGCAACAGG AACCACAGCT GCTGTGGCCA CAGCCAGAGC CACCACTGTG 
AGCCGACTGT CTGCCTCTTC TGTCACCCCA CGAGTCTACC CCAGTTACAC CAGCCGGCCT 
GTTGGCAGAA CTACGGCACC GCTAACCACT CGCCGGCCAC CAACCACTAC CGCCAGTATT 
GACCGACCTC GGACTCCAGG CCCGCAACGG CCCCCAAAGT CCTGTGATTC CCAGCCTTGC 
CTCCACGGAG GTACCTGCCA GGACCTGGAT TCTGGCAAGG GTTTCAGCTG CAGCTGTACT 
GCAGGCAGGG CTGGCACTGT CTGTGAGAAA GTGCAGCTCC CCTCTGTGCC AGCTTTTAAG 
GGCCACTCCT TCTTGGCCTT CCCCACCCTC CGAGCCTACC ACACGCTGCG TCTGGCACTA 
GAATTCCGGG CGCTGGAGAC AGAGGGACTG CTGCTCTACA ATGGCAATGC ACGTGGCAAA 
GATTTCCTGG CTCTGGCTCT GTTGGATGGT CATGTACAGT TCAGGTTCGA CACGGGCTCA 
GGGCCGGCGG TGCTAACAAG CTTAGTGCCA GTGGAACCGG GACGGTGGCA CCGCCTCGAG 
TTGTCACGGC ATTGGCGGCA GGGCACACTT TCTGTGGATG GCGAGGCTCC TGTTGTAGGT 
GAAAGTCCGA GTGGCACTGA TGGCCTCAAC TTGGACACGA AGCTCTATGT GGGTGGTCTC 
CCAGAAGAAC AAGTTGCCAC GGTGCTTGAT CGGACCTCTG TGGGCATCGG CCTGAAAGGA 
TGCATTCGTA TGTTGGACAT CAACAACCAG CAGCTGGAGC TGAGCGATTG GCAGAGGGCT 
GTGGTTCAAA GCTCTGGTGT GGGGGAATGC GGAGACCATC CCTGCTCACC TAACCCCTGC 
CATGGCGGGG CCCTCTGCCA GGCCCTGGAG GCTGGCGTGT TCCTCTGTCA GTGCCCACCT 
GGCCGCTTTG GCCCAACTTG TGCAGATGAA AAGAACCCCT GCCAACCGAA CCCCTGCCAC 
GGGTCAGCCC CCTGCCATGT GCTTTCCAGG GGTGGGGCCA AGTGTGCGTG CCCCCTGGGA 
CGCAGTGGTT CCTTCTGTGA GACAGTCCTG GAGAATGCTG GCTCCCGGCC CTTCCTGGCT 
GACTTTAATG GCTTCTCCTA CCTGGAACTG AAAGGCTTGC ACACCTTCGA GAGAGACCTA 
GGGGAGAAGA TGGCGCTGGA GATGGTGTTC TTGGCTCGTG GGCCCAGTGG CTTACTCCTC 
TACAATGGGC AGAAGACGGA TGGCAAGGGG GACTTTGTAT CCCTGGCCCT GCATAACCGG 
CACCTAGAGT TCCGCTATGA CCTTGGCAAG GGGGCTGCAA TCATCAGGAG CAAAGAGCCC 
ATAGCCCTGG GCACCTGGGT TAGGGTATTC CTGGAACGAA ATGGCCGCAA GGGTGCCCTT 
CAAGTGGGTG ATGGGCCCCG TGTGCTAGGG GAATCTCCGA AATCCCGCAA GGTCCCGCAC 
ACCATGCTCA ACCTCAAGGA GCCCCTCTAT GTGGGGGGAG CTCCTGACTT CAGCAAGCTG 
GCTCGGGGCG CTGCAGTGGC CTCCGGCTTT GATGGTGCCA TCCAGCTGGT GTCTCTAAGA 
GGCCATCAGC TGCTGACTCA GGAGCATGTG TTGCGGGCAG TAGATGTAGC GCCTTTTGCA 
GGCCACCCTT GTACCCAGGC CGTGGACAAC CCCTGCCTTA ATGGGGGCTC CTGTATCCCG 
AGGGAAGCCA CTTATGAGTG CCTGTGTCCT GGGGGCTTCT CTGGGCTGCA CTGCGAGAAG 
GGGATAGTTG AGAAGTCAGT GGGGGACCTA GAAACACTGG CCTTTGATGG GCGGACCTAC 
ATCGAGTACC TCAATGCTGT GACTGAGAGT GAGAAAGCGC TGCAGAGCAA CCACTTTGAG 
CTGAGCTTAC GCACTGAGGC CACGCAGGGG CTGGTGCTGT GGATTGGAAA GGTTGGAGAA 
CGTGCAGACT ACATGGCTCT GGCCATTGTG GATGGGCACC TACAACTGAG CTATGACCTA 
GGCTCCCAGC CAGTTGTGCT GCGCTCCACT GTGAAGGTCA ACACCAACCG CTGGCTTCGA 
GTCAGGGCTC ACAGGGAGCA CAGGGAAGGT TCCCTTCAGG TGGGCAATGA AGCCCCTGTG 
ACTGGCTCTT CCCCGCTGGG TGCCACACAA TTGGACACAG ATGGAGCCCT GTGGCTTGGA 
GGCCTACAGA AGCTTCCTGT GGGGCAGGCT CTCCCCAAGG CCTATGGCAC GGGTTTTGTG 
GGCTGTCTGC GGGACGTGGT AGTGGGCCAT CGCCAGCTGC ATCTGCTGGA GGACGCTGTC 
ACCAAACCAG AGCTAAGACC CTGCCCCACT CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006538496.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538559.1 Mus musculus agrin (Agrn), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu33958
Clone ID Related Accession (Same CDS sequence)	XM_006538496.3 , XM_006538496.4
Accession Version	XM_006538496.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	agrin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCTG ATGTGTGCAG GGGAATGTTA TGTGGCTTTG GTGCTGTGTG TGAACCTAGT 
GTTGAGGATC CAGGCCGGGC CTCCTGTGTG TGCAAGAAGA ATGTCTGCCC TGCTATGGTA 
GCTCCTGTGT GTGGCTCAGA TGCTTCCACC TATAGCAACG AGTGTGAGCT ACAGCGTGCA 
CAGTGCAACC AGCAACGGCG CATCCGCCTA CTCCGCCAAG GGCCATGTGG GTCCCGGGAC 
CCCTGTGCCA ATGTGACCTG CAGTTTCGGT AGTACCTGTG TACCTTCAGC CGATGGACAG 
ACCGCCTCGT GTCTGTGTCC TACAACCTGC TTTGGGGCCC CTGATGGCAC AGTGTGTGGC 
AGTGATGGTG TTGACTACCC TAGTGAGTGC CAGCTGCTCC GTCATGCCTG TGCCAACCAG 
GAGCACATCT TTAAGAAGTT CGATGGTCCT TGTGACCCCT GCCAGGGCAG CATGTCAGAC 
CTGAATCATA TTTGCCGGGT GAACCCACGT ACACGGCACC CAGAAATGCT TCTGCGGCCT 
GAGAACTGCC CCGCCCAACA CACACCTATC TGTGGAGATG ATGGGGTCAC CTATGAAAAC 
GACTGTGTCA TGAGCCGTAT AGGTGCAGCC CGTGGCCTGC TTCTCCAGAA AGTGCGCTCT 
GGTCAATGCC AGACTCGAGA CCAGTGCCCG GAGACCTGCC AGTTTAACTC TGTATGCCTG 
TCCCGCCGCG GCCGTCCCCA CTGTTCCTGC GATCGCGTCA CCTGTGATGG GGCTTACAGG 
CCAGTGTGTG CCCAGGATGG GCACACGTAT GACAATGACT GTTGGCGCCA ACAGGCCGAG 
TGTCGACAAC AGCAGACCAT TCCCCCCAAG CACCAGGGCC CGTGTGACCA GACCCCATCC 
CCGTGCCGTG GAGCGCAGTG TGCATTTGGG GCAACATGCA CAGTGAAGAA TGGGAAAGCT 
GTGTGCGAGT GCCAGCGGGT GTGCTCGGGC GGCTACGATC CTGTGTGCGG CAGTGATGGT 
GTCACTTACG GCAGTGTGTG CGAGCTGGAA TCCATGGCCT GTACCCTTGG GCGGGAAATC 
CGAGTGGCCC GCAGAGGACC GTGTGACCGA TGTGGGCAGT GCCGGTTTGG ATCCTTGTGC 
GAGGTGGAGA CTGGACGCTG TGTGTGCCCC TCTGAGTGTG TGGAGTCAGC CCAGCCCGTA 
TGTGGCTCTG ACGGACACAC ATATGCTAGT GAATGTGAGC TGCATGTCCA CGCCTGTACA 
CACCAGATCA GCCTATACGT GGCCTCAGCC GGACACTGCC AGACCTGTGG AGAAACAGTT 
TGTACCTTTG GGGCTGTGTG CTCAGCTGGA CAGTGTGTAT GTCCCCGTTG TGAGCACCCC 
CCACCTGGCC CTGTGTGCGG CAGTGATGGC GTCACCTACC TCAGTGCCTG TGAGCTCCGA 
GAGGCTGCCT GTCAGCAGCA GGTACAAATT GAGGAGGCCC GTGCAGGGCC GTGTGAGCCG 
GCTGAGTGTG GCTCAGGGGG CTCTGGGTCT GGGGAAGACA ATGCGTGTGA GCAGGAGCTG 
TGTCGGCAGC ATGGTGGTGT CTGGGATGAG GACTCAGAAG ACGGGCCGTG TGTCTGTGAC 
TTTAGTTGCC AGAGTGTCCT TAAAAGCCCA GTGTGTGGCT CAGATGGAGT CACCTATAGC 
ACGGAGTGCC ATCTGAAGAA GGCCAGATGT GAAGCGCGGC AAGAGCTGTA CGTCGCTGCT 
CAGGGAGCCT GCCGGGGCCC TACCTTGGCT CCACTGCTAC CTATGGCCTC CCCACACTGT 
GCCCAAACCC CCTATGGCTG CTGCCAGGAC AATGTCACTG CTGCCCAGGG TGTGGGCTTG 
GCTGGCTGTC CCAGCACCTG CCATTGCAAC CCACACGGCT CCTATAGCGG CACTTGTGAC 
CCAGTCACAG GGCAGTGCTC CTGCCGACCA GGTGTAGGAG GCCTCAGGTG TGATCGCTGT 
GAGCCTGGCT TCTGGAACTT CCGTGGCATT GTCACCGATG GACATAGTGG TTGCACTCCC 
TGCAGCTGTG ACCCTCGGGG TGCTGTAAGA GATGACTGTG AGCAGATGAC TGGATTGTGT 
TCCTGTAGAC CTGGTGTGGC TGGTCCCAAG TGTGGGCAGT GTCCAGATGG TCAAGCCCTG 
GGCCATCTTG GCTGTGAAGC AGATCCCACA ACACCAGTGA CTTGTGTGGA AATGCACTGT 
GAGTTTGGCG CCTCCTGCGT AGAGGAGGCT GGTTTTGCCC AGTGTGTCTG CCCAACTCTC 
ACATGTCCAG AGGCTAACTC TACCAAGGTC TGTGGATCAG ATGGTGTCAC ATACGGCAAT 
GAATGCCAGC TGAAGACCAT TGCCTGCCGC CAGCGTCTGG ACATCTCCAT TCAGAGTCTT 
GGTCCATGCC GGGAGAGTGT TGCTCCTGGG GTTTCCCCTA CATCTGCATC TATGACCACC 
CCAAGGCATA TCCTGAGCAG GACACTGGCG TCTCCCCACA GCAGCCTTCC TCTGTCTCCC 
AGCACTACTG CCCATGATTG GCCCACCCCA TTACCCACAT CACCTCAGAC CGTAGTCGGC 
ACCCCCAGGA GCACTGCAGC CACACCCTCT GATGTGGCCA GTCTTGCTAC AGCGATCTTC 
AGGGAATCTG GCAGCACCAA CGGCAGTGGC GATGAGGAGC TCAGTGGCGA TGAGGAGGCC 
AGTGGGGGCG GGTCTGGGGG ACTTGAGCCC CCGGTGGGCA GCGTTGTGGT GACCCACGGG 
CCACCCATCG AGAGGGCTTC CTGTTACAAC TCACCTTTGG GCTGCTGCTC AGATGGCAAG 
ACACCCTCAC TGGACTCAGA AGGCTCCAAC TGTCCAGCTA CCAAGGCATT CCAGGGCGTG 
CTGGAGCTTG AGGGGGTCGA GGGACAGGAA CTGTTCTACA CACCAGAGAT GGCTGACCCC 
AAGTCAGAGT TGTTTGGGGA GACTGCAAGG AGCATTGAGA GCACGCTGGA CGACCTGTTC 
CGGAATTCGG ATGTTAAGAA GGACTTCTGG AGCATCCGCC TACGGGAACT GGGGCCTGGC 
AAATTAGTCC GTGCCATTGT GGATGTTCAC TTTGACCCCA CCACAGCCTT CCAGGCACCA 
GATGTGGGTC AGGCCTTGCT CCAACAGATC CAGGTATCCA GGCCGTGGGC CCTGGCAGTG 
AGGAGGCCTC TGCGGGAGCA TGTGCGATTC TTGGACTTTG ACTGGTTTCC CACTTTTTTT 
ACGGGAGCTG CAACAGGAAC CACAGCTGCT GTGGCCACAG CCAGAGCCAC CACTGTGAGC 
CGACTGTCTG CCTCTTCTGT CACCCCACGA GTCTACCCCA GTTACACCAG CCGGCCTGTT 
GGCAGAACTA CGGCACCGCT AACCACTCGC CGGCCACCAA CCACTACCGC CAGTATTGAC 
CGACCTCGGA CTCCAGGCCC GCAACGGCCC CCAAAGTCCT GTGATTCCCA GCCTTGCCTC 
CACGGAGGTA CCTGCCAGGA CCTGGATTCT GGCAAGGGTT TCAGCTGCAG CTGTACTGCA 
GGCAGGGCTG GCACTGTCTG TGAGAAAGTG CAGCTCCCCT CTGTGCCAGC TTTTAAGGGC 
CACTCCTTCT TGGCCTTCCC CACCCTCCGA GCCTACCACA CGCTGCGTCT GGCACTAGAA 
TTCCGGGCGC TGGAGACAGA GGGACTGCTG CTCTACAATG GCAATGCACG TGGCAAAGAT 
TTCCTGGCTC TGGCTCTGTT GGATGGTCAT GTACAGTTCA GGTTCGACAC GGGCTCAGGG 
CCGGCGGTGC TAACAAGCTT AGTGCCAGTG GAACCGGGAC GGTGGCACCG CCTCGAGTTG 
TCACGGCATT GGCGGCAGGG CACACTTTCT GTGGATGGCG AGGCTCCTGT TGTAGGTGAA 
AGTCCGAGTG GCACTGATGG CCTCAACTTG GACACGAAGC TCTATGTGGG TGGTCTCCCA 
GAAGAACAAG TTGCCACGGT GCTTGATCGG ACCTCTGTGG GCATCGGCCT GAAAGGATGC 
ATTCGTATGT TGGACATCAA CAACCAGCAG CTGGAGCTGA GCGATTGGCA GAGGGCTGTG 
GTTCAAAGCT CTGGTGTGGG GGAATGCGGA GACCATCCCT GCTCACCTAA CCCCTGCCAT 
GGCGGGGCCC TCTGCCAGGC CCTGGAGGCT GGCGTGTTCC TCTGTCAGTG CCCACCTGGC 
CGCTTTGGCC CAACTTGTGC AGATGAAAAG AACCCCTGCC AACCGAACCC CTGCCACGGG 
TCAGCCCCCT GCCATGTGCT TTCCAGGGGT GGGGCCAAGT GTGCGTGCCC CCTGGGACGC 
AGTGGTTCCT TCTGTGAGAC AGTCCTGGAG AATGCTGGCT CCCGGCCCTT CCTGGCTGAC 
TTTAATGGCT TCTCCTACCT GGAACTGAAA GGCTTGCACA CCTTCGAGAG AGACCTAGGG 
GAGAAGATGG CGCTGGAGAT GGTGTTCTTG GCTCGTGGGC CCAGTGGCTT ACTCCTCTAC 
AATGGGCAGA AGACGGATGG CAAGGGGGAC TTTGTATCCC TGGCCCTGCA TAACCGGCAC 
CTAGAGTTCC GCTATGACCT TGGCAAGGGG GCTGCAATCA TCAGGAGCAA AGAGCCCATA 
GCCCTGGGCA CCTGGGTTAG GGTATTCCTG GAACGAAATG GCCGCAAGGG TGCCCTTCAA 
GTGGGTGATG GGCCCCGTGT GCTAGGGGAA TCTCCGAAAT CCCGCAAGGT CCCGCACACC 
ATGCTCAACC TCAAGGAGCC CCTCTATGTG GGGGGAGCTC CTGACTTCAG CAAGCTGGCT 
CGGGGCGCTG CAGTGGCCTC CGGCTTTGAT GGTGCCATCC AGCTGGTGTC TCTAAGAGGC 
CATCAGCTGC TGACTCAGGA GCATGTGTTG CGGGCAGTAG ATGTAGCGCC TTTTGCAGGC 
CACCCTTGTA CCCAGGCCGT GGACAACCCC TGCCTTAATG GGGGCTCCTG TATCCCGAGG 
GAAGCCACTT ATGAGTGCCT GTGTCCTGGG GGCTTCTCTG GGCTGCACTG CGAGAAGGGG 
ATAGTTGAGA AGTCAGTGGG GGACCTAGAA ACACTGGCCT TTGATGGGCG GACCTACATC 
GAGTACCTCA ATGCTGTGAC TGAGAGCGAG CTGACCAATG AGATCCCAGC CCCCGAAACT 
CTGGATTCCC GGGCCCTTTT CAGTGAGAAA GCGCTGCAGA GCAACCACTT TGAGCTGAGC 
TTACGCACTG AGGCCACGCA GGGGCTGGTG CTGTGGATTG GAAAGGTTGG AGAACGTGCA 
GACTACATGG CTCTGGCCAT TGTGGATGGG CACCTACAAC TGAGCTATGA CCTAGGCTCC 
CAGCCAGTTG TGCTGCGCTC CACTGTGAAG GTCAACACCA ACCGCTGGCT TCGAGTCAGG 
GCTCACAGGG AGCACAGGGA AGGTTCCCTT CAGGTGGGCA ATGAAGCCCC TGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAATTGGAC ACAGATGGAG CCCTGTGGCT TGGAGGCCTA 
CAGAAGCTTC CTGTGGGGCA GGCTCTCCCC AAGGCCTATG GCACGGGTTT TGTGGGCTGT 
CTGCGGGACG TGGTAGTGGG CCATCGCCAG CTGCATCTGC TGGAGGACGC TGTCACCAAA 
CCAGAGCTAA GACCCTGCCC CACTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538559.1
CDS	179..5848
Translation	MPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_006538496.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538496.3

ORF Insert Sequence:

ATGCCTCCTG ATGTGTGCAG GGGAATGTTA TGTGGCTTTG GTGCTGTGTG TGAACCTAGT 
GTTGAGGATC CAGGCCGGGC CTCCTGTGTG TGCAAGAAGA ATGTCTGCCC TGCTATGGTA 
GCTCCTGTGT GTGGCTCAGA TGCTTCCACC TATAGCAACG AGTGTGAGCT ACAGCGTGCA 
CAGTGCAACC AGCAACGGCG CATCCGCCTA CTCCGCCAAG GGCCATGTGG GTCCCGGGAC 
CCCTGTGCCA ATGTGACCTG CAGTTTCGGT AGTACCTGTG TACCTTCAGC CGATGGACAG 
ACCGCCTCGT GTCTGTGTCC TACAACCTGC TTTGGGGCCC CTGATGGCAC AGTGTGTGGC 
AGTGATGGTG TTGACTACCC TAGTGAGTGC CAGCTGCTCC GTCATGCCTG TGCCAACCAG 
GAGCACATCT TTAAGAAGTT CGATGGTCCT TGTGACCCCT GCCAGGGCAG CATGTCAGAC 
CTGAATCATA TTTGCCGGGT GAACCCACGT ACACGGCACC CAGAAATGCT TCTGCGGCCT 
GAGAACTGCC CCGCCCAACA CACACCTATC TGTGGAGATG ATGGGGTCAC CTATGAAAAC 
GACTGTGTCA TGAGCCGTAT AGGTGCAGCC CGTGGCCTGC TTCTCCAGAA AGTGCGCTCT 
GGTCAATGCC AGACTCGAGA CCAGTGCCCG GAGACCTGCC AGTTTAACTC TGTATGCCTG 
TCCCGCCGCG GCCGTCCCCA CTGTTCCTGC GATCGCGTCA CCTGTGATGG GGCTTACAGG 
CCAGTGTGTG CCCAGGATGG GCACACGTAT GACAATGACT GTTGGCGCCA ACAGGCCGAG 
TGTCGACAAC AGCAGACCAT TCCCCCCAAG CACCAGGGCC CGTGTGACCA GACCCCATCC 
CCGTGCCGTG GAGCGCAGTG TGCATTTGGG GCAACATGCA CAGTGAAGAA TGGGAAAGCT 
GTGTGCGAGT GCCAGCGGGT GTGCTCGGGC GGCTACGATC CTGTGTGCGG CAGTGATGGT 
GTCACTTACG GCAGTGTGTG CGAGCTGGAA TCCATGGCCT GTACCCTTGG GCGGGAAATC 
CGAGTGGCCC GCAGAGGACC GTGTGACCGA TGTGGGCAGT GCCGGTTTGG ATCCTTGTGC 
GAGGTGGAGA CTGGACGCTG TGTGTGCCCC TCTGAGTGTG TGGAGTCAGC CCAGCCCGTA 
TGTGGCTCTG ACGGACACAC ATATGCTAGT GAATGTGAGC TGCATGTCCA CGCCTGTACA 
CACCAGATCA GCCTATACGT GGCCTCAGCC GGACACTGCC AGACCTGTGG AGAAACAGTT 
TGTACCTTTG GGGCTGTGTG CTCAGCTGGA CAGTGTGTAT GTCCCCGTTG TGAGCACCCC 
CCACCTGGCC CTGTGTGCGG CAGTGATGGC GTCACCTACC TCAGTGCCTG TGAGCTCCGA 
GAGGCTGCCT GTCAGCAGCA GGTACAAATT GAGGAGGCCC GTGCAGGGCC GTGTGAGCCG 
GCTGAGTGTG GCTCAGGGGG CTCTGGGTCT GGGGAAGACA ATGCGTGTGA GCAGGAGCTG 
TGTCGGCAGC ATGGTGGTGT CTGGGATGAG GACTCAGAAG ACGGGCCGTG TGTCTGTGAC 
TTTAGTTGCC AGAGTGTCCT TAAAAGCCCA GTGTGTGGCT CAGATGGAGT CACCTATAGC 
ACGGAGTGCC ATCTGAAGAA GGCCAGATGT GAAGCGCGGC AAGAGCTGTA CGTCGCTGCT 
CAGGGAGCCT GCCGGGGCCC TACCTTGGCT CCACTGCTAC CTATGGCCTC CCCACACTGT 
GCCCAAACCC CCTATGGCTG CTGCCAGGAC AATGTCACTG CTGCCCAGGG TGTGGGCTTG 
GCTGGCTGTC CCAGCACCTG CCATTGCAAC CCACACGGCT CCTATAGCGG CACTTGTGAC 
CCAGTCACAG GGCAGTGCTC CTGCCGACCA GGTGTAGGAG GCCTCAGGTG TGATCGCTGT 
GAGCCTGGCT TCTGGAACTT CCGTGGCATT GTCACCGATG GACATAGTGG TTGCACTCCC 
TGCAGCTGTG ACCCTCGGGG TGCTGTAAGA GATGACTGTG AGCAGATGAC TGGATTGTGT 
TCCTGTAGAC CTGGTGTGGC TGGTCCCAAG TGTGGGCAGT GTCCAGATGG TCAAGCCCTG 
GGCCATCTTG GCTGTGAAGC AGATCCCACA ACACCAGTGA CTTGTGTGGA AATGCACTGT 
GAGTTTGGCG CCTCCTGCGT AGAGGAGGCT GGTTTTGCCC AGTGTGTCTG CCCAACTCTC 
ACATGTCCAG AGGCTAACTC TACCAAGGTC TGTGGATCAG ATGGTGTCAC ATACGGCAAT 
GAATGCCAGC TGAAGACCAT TGCCTGCCGC CAGCGTCTGG ACATCTCCAT TCAGAGTCTT 
GGTCCATGCC GGGAGAGTGT TGCTCCTGGG GTTTCCCCTA CATCTGCATC TATGACCACC 
CCAAGGCATA TCCTGAGCAG GACACTGGCG TCTCCCCACA GCAGCCTTCC TCTGTCTCCC 
AGCACTACTG CCCATGATTG GCCCACCCCA TTACCCACAT CACCTCAGAC CGTAGTCGGC 
ACCCCCAGGA GCACTGCAGC CACACCCTCT GATGTGGCCA GTCTTGCTAC AGCGATCTTC 
AGGGAATCTG GCAGCACCAA CGGCAGTGGC GATGAGGAGC TCAGTGGCGA TGAGGAGGCC 
AGTGGGGGCG GGTCTGGGGG ACTTGAGCCC CCGGTGGGCA GCGTTGTGGT GACCCACGGG 
CCACCCATCG AGAGGGCTTC CTGTTACAAC TCACCTTTGG GCTGCTGCTC AGATGGCAAG 
ACACCCTCAC TGGACTCAGA AGGCTCCAAC TGTCCAGCTA CCAAGGCATT CCAGGGCGTG 
CTGGAGCTTG AGGGGGTCGA GGGACAGGAA CTGTTCTACA CACCAGAGAT GGCTGACCCC 
AAGTCAGAGT TGTTTGGGGA GACTGCAAGG AGCATTGAGA GCACGCTGGA CGACCTGTTC 
CGGAATTCGG ATGTTAAGAA GGACTTCTGG AGCATCCGCC TACGGGAACT GGGGCCTGGC 
AAATTAGTCC GTGCCATTGT GGATGTTCAC TTTGACCCCA CCACAGCCTT CCAGGCACCA 
GATGTGGGTC AGGCCTTGCT CCAACAGATC CAGGTATCCA GGCCGTGGGC CCTGGCAGTG 
AGGAGGCCTC TGCGGGAGCA TGTGCGATTC TTGGACTTTG ACTGGTTTCC CACTTTTTTT 
ACGGGAGCTG CAACAGGAAC CACAGCTGCT GTGGCCACAG CCAGAGCCAC CACTGTGAGC 
CGACTGTCTG CCTCTTCTGT CACCCCACGA GTCTACCCCA GTTACACCAG CCGGCCTGTT 
GGCAGAACTA CGGCACCGCT AACCACTCGC CGGCCACCAA CCACTACCGC CAGTATTGAC 
CGACCTCGGA CTCCAGGCCC GCAACGGCCC CCAAAGTCCT GTGATTCCCA GCCTTGCCTC 
CACGGAGGTA CCTGCCAGGA CCTGGATTCT GGCAAGGGTT TCAGCTGCAG CTGTACTGCA 
GGCAGGGCTG GCACTGTCTG TGAGAAAGTG CAGCTCCCCT CTGTGCCAGC TTTTAAGGGC 
CACTCCTTCT TGGCCTTCCC CACCCTCCGA GCCTACCACA CGCTGCGTCT GGCACTAGAA 
TTCCGGGCGC TGGAGACAGA GGGACTGCTG CTCTACAATG GCAATGCACG TGGCAAAGAT 
TTCCTGGCTC TGGCTCTGTT GGATGGTCAT GTACAGTTCA GGTTCGACAC GGGCTCAGGG 
CCGGCGGTGC TAACAAGCTT AGTGCCAGTG GAACCGGGAC GGTGGCACCG CCTCGAGTTG 
TCACGGCATT GGCGGCAGGG CACACTTTCT GTGGATGGCG AGGCTCCTGT TGTAGGTGAA 
AGTCCGAGTG GCACTGATGG CCTCAACTTG GACACGAAGC TCTATGTGGG TGGTCTCCCA 
GAAGAACAAG TTGCCACGGT GCTTGATCGG ACCTCTGTGG GCATCGGCCT GAAAGGATGC 
ATTCGTATGT TGGACATCAA CAACCAGCAG CTGGAGCTGA GCGATTGGCA GAGGGCTGTG 
GTTCAAAGCT CTGGTGTGGG GGAATGCGGA GACCATCCCT GCTCACCTAA CCCCTGCCAT 
GGCGGGGCCC TCTGCCAGGC CCTGGAGGCT GGCGTGTTCC TCTGTCAGTG CCCACCTGGC 
CGCTTTGGCC CAACTTGTGC AGATGAAAAG AACCCCTGCC AACCGAACCC CTGCCACGGG 
TCAGCCCCCT GCCATGTGCT TTCCAGGGGT GGGGCCAAGT GTGCGTGCCC CCTGGGACGC 
AGTGGTTCCT TCTGTGAGAC AGTCCTGGAG AATGCTGGCT CCCGGCCCTT CCTGGCTGAC 
TTTAATGGCT TCTCCTACCT GGAACTGAAA GGCTTGCACA CCTTCGAGAG AGACCTAGGG 
GAGAAGATGG CGCTGGAGAT GGTGTTCTTG GCTCGTGGGC CCAGTGGCTT ACTCCTCTAC 
AATGGGCAGA AGACGGATGG CAAGGGGGAC TTTGTATCCC TGGCCCTGCA TAACCGGCAC 
CTAGAGTTCC GCTATGACCT TGGCAAGGGG GCTGCAATCA TCAGGAGCAA AGAGCCCATA 
GCCCTGGGCA CCTGGGTTAG GGTATTCCTG GAACGAAATG GCCGCAAGGG TGCCCTTCAA 
GTGGGTGATG GGCCCCGTGT GCTAGGGGAA TCTCCGAAAT CCCGCAAGGT CCCGCACACC 
ATGCTCAACC TCAAGGAGCC CCTCTATGTG GGGGGAGCTC CTGACTTCAG CAAGCTGGCT 
CGGGGCGCTG CAGTGGCCTC CGGCTTTGAT GGTGCCATCC AGCTGGTGTC TCTAAGAGGC 
CATCAGCTGC TGACTCAGGA GCATGTGTTG CGGGCAGTAG ATGTAGCGCC TTTTGCAGGC 
CACCCTTGTA CCCAGGCCGT GGACAACCCC TGCCTTAATG GGGGCTCCTG TATCCCGAGG 
GAAGCCACTT ATGAGTGCCT GTGTCCTGGG GGCTTCTCTG GGCTGCACTG CGAGAAGGGG 
ATAGTTGAGA AGTCAGTGGG GGACCTAGAA ACACTGGCCT TTGATGGGCG GACCTACATC 
GAGTACCTCA ATGCTGTGAC TGAGAGCGAG CTGACCAATG AGATCCCAGC CCCCGAAACT 
CTGGATTCCC GGGCCCTTTT CAGTGAGAAA GCGCTGCAGA GCAACCACTT TGAGCTGAGC 
TTACGCACTG AGGCCACGCA GGGGCTGGTG CTGTGGATTG GAAAGGTTGG AGAACGTGCA 
GACTACATGG CTCTGGCCAT TGTGGATGGG CACCTACAAC TGAGCTATGA CCTAGGCTCC 
CAGCCAGTTG TGCTGCGCTC CACTGTGAAG GTCAACACCA ACCGCTGGCT TCGAGTCAGG 
GCTCACAGGG AGCACAGGGA AGGTTCCCTT CAGGTGGGCA ATGAAGCCCC TGTGACTGGC 
TCTTCCCCGC TGGGTGCCAC ACAATTGGAC ACAGATGGAG CCCTGTGGCT TGGAGGCCTA 
CAGAAGCTTC CTGTGGGGCA GGCTCTCCCC AAGGCCTATG GCACGGGTTT TGTGGGCTGT 
CTGCGGGACG TGGTAGTGGG CCATCGCCAG CTGCATCTGC TGGAGGACGC TGTCACCAAA 
CCAGAGCTAA GACCCTGCCC CACTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_011250176.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248478.1 Mus musculus agrin (Agrn), transcript variant X2, mRNA.

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CloneID	OMu67084
Clone ID Related Accession (Same CDS sequence)	XM_011250176.2 , XM_011250176.1
Accession Version	XM_011250176.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
GTCCCGCACA CCATGCTCAA CCTCAAGGAG CCCCTCTATG TGGGGGGAGC TCCTGACTTC 
AGCAAGCTGG CTCGGGGCGC TGCAGTGGCC TCCGGCTTTG ATGGTGCCAT CCAGCTGGTG 
TCTCTAAGAG GCCATCAGCT GCTGACTCAG GAGCATGTGT TGCGGGCAGT AGATGTAGCG 
CCTTTTGCAG GCCACCCTTG TACCCAGGCC GTGGACAACC CCTGCCTTAA TGGGGGCTCC 
TGTATCCCGA GGGAAGCCAC TTATGAGTGC CTGTGTCCTG GGGGCTTCTC TGGGCTGCAC 
TGCGAGAAGG GGATAGTTGA GAAGTCAGTG GGGGACCTAG AAACACTGGC CTTTGATGGG 
CGGACCTACA TCGAGTACCT CAATGCTGTG ACTGAGAGCG AGCTGACCAA TGAGATCCCA 
GCCCCCGAAA CTCTGGATTC CCGGGCCCTT TTCAGTGAGA AAGCGCTGCA GAGCAACCAC 
TTTGAGCTGA GCTTACGCAC TGAGGCCACG CAGGGGCTGG TGCTGTGGAT TGGAAAGGTT 
GGAGAACGTG CAGACTACAT GGCTCTGGCC ATTGTGGATG GGCACCTACA ACTGAGCTAT 
GACCTAGGCT CCCAGCCAGT TGTGCTGCGC TCCACTGTGA AGGTCAACAC CAACCGCTGG 
CTTCGAGTCA GGGCTCACAG GGAGCACAGG GAAGGTTCCC TTCAGGTGGG CAATGAAGCC 
CCTGTGACTG GCTCTTCCCC GCTGGGTGCC ACACAATTGG ACACAGATGG AGCCCTGTGG 
CTTGGAGGCC TACAGAAGCT TCCTGTGGGG CAGGCTCTCC CCAAGGCCTA TGGCACGGGT 
TTTGTGGGCT GTCTGCGGGA CGTGGTAGTG GGCCATCGCC AGCTGCATCT GCTGGAGGAC 
GCTGTCACCA AACCAGAGCT AAGACCCTGC CCCACTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248478.1
CDS	99..6260
Translation	MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_011250176.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250176.1

ORF Insert Sequence:

ATGGTCCGCC CGCGGCTGAG CTTCCCGGCC CCGCTGCTGC CGCTGCTGCT GCTCCTGGCG 
GCGGCCGCGC CAGCCGTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CTTGGAGCGG 
CGCGAGGAGG AGGCGAACGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACCTACTC CTGCAAGGTT CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AAGAGAGCCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACCGGGGACA CTAGAATCTT CTTTGTGAAC 
CCAGCACCAC CCTACCTGTG GCCAGCCCAC AAGAATGAGT TGATGCTCAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGG 
ATCCACTTCA CAGCAGCGCC TTCTATGCCT CCTGATGTGT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GGGCCTCCTG TGTGTGCAAG 
AAGAATGTCT GCCCTGCTAT GGTAGCTCCT GTGTGTGGCT CAGATGCTTC CACCTATAGC 
AACGAGTGTG AGCTACAGCG TGCACAGTGC AACCAGCAAC GGCGCATCCG CCTACTCCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAATGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CAGCCGATGG ACAGACCGCC TCGTGTCTGT GTCCTACAAC CTGCTTTGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAT GGTGTTGACT ACCCTAGTGA GTGCCAGCTG 
CTCCGTCATG CCTGTGCCAA CCAGGAGCAC ATCTTTAAGA AGTTCGATGG TCCTTGTGAC 
CCCTGCCAGG GCAGCATGTC AGACCTGAAT CATATTTGCC GGGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCCGCCC AACACACACC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AGCCCGTGGC 
CTGCTTCTCC AGAAAGTGCG CTCTGGTCAA TGCCAGACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCTGTATG CCTGTCCCGC CGCGGCCGTC CCCACTGTTC CTGCGATCGC 
GTCACCTGTG ATGGGGCTTA CAGGCCAGTG TGTGCCCAGG ATGGGCACAC GTATGACAAT 
GACTGTTGGC GCCAACAGGC CGAGTGTCGA CAACAGCAGA CCATTCCCCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC ATCCCCGTGC CGTGGAGCGC AGTGTGCATT TGGGGCAACA 
TGCACAGTGA AGAATGGGAA AGCTGTGTGC GAGTGCCAGC GGGTGTGCTC GGGCGGCTAC 
GATCCTGTGT GCGGCAGTGA TGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCGAGTG GCCCGCAGAG GACCGTGTGA CCGATGTGGG 
CAGTGCCGGT TTGGATCCTT GTGCGAGGTG GAGACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGTGGC TCTGACGGAC ACACATATGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACACACCAG ATCAGCCTAT ACGTGGCCTC AGCCGGACAC 
TGCCAGACCT GTGGAGAAAC AGTTTGTACC TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCCCCACCT GGCCCTGTGT GCGGCAGTGA TGGCGTCACC 
TACCTCAGTG CCTGTGAGCT CCGAGAGGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCGTGCAG GGCCGTGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG GTCTGGGGAA 
GACAATGCGT GTGAGCAGGA GCTGTGTCGG CAGCATGGTG GTGTCTGGGA TGAGGACTCA 
GAAGACGGGC CGTGTGTCTG TGACTTTAGT TGCCAGAGTG TCCTTAAAAG CCCAGTGTGT 
GGCTCAGATG GAGTCACCTA TAGCACGGAG TGCCATCTGA AGAAGGCCAG ATGTGAAGCG 
CGGCAAGAGC TGTACGTCGC TGCTCAGGGA GCCTGCCGGG GCCCTACCTT GGCTCCACTG 
CTACCTATGG CCTCCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCACAC 
GGCTCCTATA GCGGCACTTG TGACCCAGTC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAGCCT GGCTTCTGGA ACTTCCGTGG CATTGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGTGACCCTC GGGGTGCTGT AAGAGATGAC 
TGTGAGCAGA TGACTGGATT GTGTTCCTGT AGACCTGGTG TGGCTGGTCC CAAGTGTGGG 
CAGTGTCCAG ATGGTCAAGC CCTGGGCCAT CTTGGCTGTG AAGCAGATCC CACAACACCA 
GTGACTTGTG TGGAAATGCA CTGTGAGTTT GGCGCCTCCT GCGTAGAGGA GGCTGGTTTT 
GCCCAGTGTG TCTGCCCAAC TCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGTGGA 
TCAGATGGTG TCACATACGG CAATGAATGC CAGCTGAAGA CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCATTCAGAG TCTTGGTCCA TGCCGGGAGA GTGTTGCTCC TGGGGTTTCC 
CCTACATCTG CATCTATGAC CACCCCAAGG CATATCCTGA GCAGGACACT GGCGTCTCCC 
CACAGCAGCC TTCCTCTGTC TCCCAGCACT ACTGCCCATG ATTGGCCCAC CCCATTACCC 
ACATCACCTC AGACCGTAGT CGGCACCCCC AGGAGCACTG CAGCCACACC CTCTGATGTG 
GCCAGTCTTG CTACAGCGAT CTTCAGGGAA TCTGGCAGCA CCAACGGCAG TGGCGATGAG 
GAGCTCAGTG GCGATGAGGA GGCCAGTGGG GGCGGGTCTG GGGGACTTGA GCCCCCGGTG 
GGCAGCGTTG TGGTGACCCA CGGGCCACCC ATCGAGAGGG CTTCCTGTTA CAACTCACCT 
TTGGGCTGCT GCTCAGATGG CAAGACACCC TCACTGGACT CAGAAGGCTC CAACTGTCCA 
GCTACCAAGG CATTCCAGGG CGTGCTGGAG CTTGAGGGGG TCGAGGGACA GGAACTGTTC 
TACACACCAG AGATGGCTGA CCCCAAGTCA GAGTTGTTTG GGGAGACTGC AAGGAGCATT 
GAGAGCACGC TGGACGACCT GTTCCGGAAT TCGGATGTTA AGAAGGACTT CTGGAGCATC 
CGCCTACGGG AACTGGGGCC TGGCAAATTA GTCCGTGCCA TTGTGGATGT TCACTTTGAC 
CCCACCACAG CCTTCCAGGC ACCAGATGTG GGTCAGGCCT TGCTCCAACA GATCCAGGTA 
TCCAGGCCGT GGGCCCTGGC AGTGAGGAGG CCTCTGCGGG AGCATGTGCG ATTCTTGGAC 
TTTGACTGGT TTCCCACTTT TTTTACGGGA GCTGCAACAG GAACCACAGC TGCTGTGGCC 
ACAGCCAGAG CCACCACTGT GAGCCGACTG TCTGCCTCTT CTGTCACCCC ACGAGTCTAC 
CCCAGTTACA CCAGCCGGCC TGTTGGCAGA ACTACGGCAC CGCTAACCAC TCGCCGGCCA 
CCAACCACTA CCGCCAGTAT TGACCGACCT CGGACTCCAG GCCCGCAACG GCCCCCAAAG 
TCCTGTGATT CCCAGCCTTG CCTCCACGGA GGTACCTGCC AGGACCTGGA TTCTGGCAAG 
GGTTTCAGCT GCAGCTGTAC TGCAGGCAGG GCTGGCACTG TCTGTGAGAA AGTGCAGCTC 
CCCTCTGTGC CAGCTTTTAA GGGCCACTCC TTCTTGGCCT TCCCCACCCT CCGAGCCTAC 
CACACGCTGC GTCTGGCACT AGAATTCCGG GCGCTGGAGA CAGAGGGACT GCTGCTCTAC 
AATGGCAATG CACGTGGCAA AGATTTCCTG GCTCTGGCTC TGTTGGATGG TCATGTACAG 
TTCAGGTTCG ACACGGGCTC AGGGCCGGCG GTGCTAACAA GCTTAGTGCC AGTGGAACCG 
GGACGGTGGC ACCGCCTCGA GTTGTCACGG CATTGGCGGC AGGGCACACT TTCTGTGGAT 
GGCGAGGCTC CTGTTGTAGG TGAAAGTCCG AGTGGCACTG ATGGCCTCAA CTTGGACACG 
AAGCTCTATG TGGGTGGTCT CCCAGAAGAA CAAGTTGCCA CGGTGCTTGA TCGGACCTCT 
GTGGGCATCG GCCTGAAAGG ATGCATTCGT ATGTTGGACA TCAACAACCA GCAGCTGGAG 
CTGAGCGATT GGCAGAGGGC TGTGGTTCAA AGCTCTGGTG TGGGGGAATG CGGAGACCAT 
CCCTGCTCAC CTAACCCCTG CCATGGCGGG GCCCTCTGCC AGGCCCTGGA GGCTGGCGTG 
TTCCTCTGTC AGTGCCCACC TGGCCGCTTT GGCCCAACTT GTGCAGATGA AAAGAACCCC 
TGCCAACCGA ACCCCTGCCA CGGGTCAGCC CCCTGCCATG TGCTTTCCAG GGGTGGGGCC 
AAGTGTGCGT GCCCCCTGGG ACGCAGTGGT TCCTTCTGTG AGACAGTCCT GGAGAATGCT 
GGCTCCCGGC CCTTCCTGGC TGACTTTAAT GGCTTCTCCT ACCTGGAACT GAAAGGCTTG 
CACACCTTCG AGAGAGACCT AGGGGAGAAG ATGGCGCTGG AGATGGTGTT CTTGGCTCGT 
GGGCCCAGTG GCTTACTCCT CTACAATGGG CAGAAGACGG ATGGCAAGGG GGACTTTGTA 
TCCCTGGCCC TGCATAACCG GCACCTAGAG TTCCGCTATG ACCTTGGCAA GGGGGCTGCA 
ATCATCAGGA GCAAAGAGCC CATAGCCCTG GGCACCTGGG TTAGGGTATT CCTGGAACGA 
AATGGCCGCA AGGGTGCCCT TCAAGTGGGT GATGGGCCCC GTGTGCTAGG GGAATCTCCG 
GTCCCGCACA CCATGCTCAA CCTCAAGGAG CCCCTCTATG TGGGGGGAGC TCCTGACTTC 
AGCAAGCTGG CTCGGGGCGC TGCAGTGGCC TCCGGCTTTG ATGGTGCCAT CCAGCTGGTG 
TCTCTAAGAG GCCATCAGCT GCTGACTCAG GAGCATGTGT TGCGGGCAGT AGATGTAGCG 
CCTTTTGCAG GCCACCCTTG TACCCAGGCC GTGGACAACC CCTGCCTTAA TGGGGGCTCC 
TGTATCCCGA GGGAAGCCAC TTATGAGTGC CTGTGTCCTG GGGGCTTCTC TGGGCTGCAC 
TGCGAGAAGG GGATAGTTGA GAAGTCAGTG GGGGACCTAG AAACACTGGC CTTTGATGGG 
CGGACCTACA TCGAGTACCT CAATGCTGTG ACTGAGAGCG AGCTGACCAA TGAGATCCCA 
GCCCCCGAAA CTCTGGATTC CCGGGCCCTT TTCAGTGAGA AAGCGCTGCA GAGCAACCAC 
TTTGAGCTGA GCTTACGCAC TGAGGCCACG CAGGGGCTGG TGCTGTGGAT TGGAAAGGTT 
GGAGAACGTG CAGACTACAT GGCTCTGGCC ATTGTGGATG GGCACCTACA ACTGAGCTAT 
GACCTAGGCT CCCAGCCAGT TGTGCTGCGC TCCACTGTGA AGGTCAACAC CAACCGCTGG 
CTTCGAGTCA GGGCTCACAG GGAGCACAGG GAAGGTTCCC TTCAGGTGGG CAATGAAGCC 
CCTGTGACTG GCTCTTCCCC GCTGGGTGCC ACACAATTGG ACACAGATGG AGCCCTGTGG 
CTTGGAGGCC TACAGAAGCT TCCTGTGGGG CAGGCTCTCC CCAAGGCCTA TGGCACGGGT 
TTTGTGGGCT GTCTGCGGGA CGTGGTAGTG GGCCATCGCC AGCTGCATCT GCTGGAGGAC 
GCTGTCACCA AACCAGAGCT AAGACCCTGC CCCACTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_011250177.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248479.1 Mus musculus agrin (Agrn), transcript variant X6, mRNA.

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CloneID	OMu67085
Clone ID Related Accession (Same CDS sequence)	XM_011250177.2 , NM_001369026.1
Accession Version	XM_011250177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGCAGC CTGGTGCCTC CGTGCTGGTT 
CGGTACTTCA TGATCCCCTG CAACATCTGC TTGATCCTCT TGGCTACTTC TACGTTGGGC 
TTTGCGGTGC TGCTTTTCCT CAGCAACTAC AAACCTGGGA TCCACTTCAC AGCAGCGCCT 
TCTATGCCTC CTGATGTGTG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG GGCCTCCTGT GTGTGCAAGA AGAATGTCTG CCCTGCTATG 
GTAGCTCCTG TGTGTGGCTC AGATGCTTCC ACCTATAGCA ACGAGTGTGA GCTACAGCGT 
GCACAGTGCA ACCAGCAACG GCGCATCCGC CTACTCCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAATGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC AGCCGATGGA 
CAGACCGCCT CGTGTCTGTG TCCTACAACC TGCTTTGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGATG GTGTTGACTA CCCTAGTGAG TGCCAGCTGC TCCGTCATGC CTGTGCCAAC 
CAGGAGCACA TCTTTAAGAA GTTCGATGGT CCTTGTGACC CCTGCCAGGG CAGCATGTCA 
GACCTGAATC ATATTTGCCG GGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCCGCCCA ACACACACCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA GCCCGTGGCC TGCTTCTCCA GAAAGTGCGC 
TCTGGTCAAT GCCAGACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCTGTATGC 
CTGTCCCGCC GCGGCCGTCC CCACTGTTCC TGCGATCGCG TCACCTGTGA TGGGGCTTAC 
AGGCCAGTGT GTGCCCAGGA TGGGCACACG TATGACAATG ACTGTTGGCG CCAACAGGCC 
GAGTGTCGAC AACAGCAGAC CATTCCCCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCA 
TCCCCGTGCC GTGGAGCGCA GTGTGCATTT GGGGCAACAT GCACAGTGAA GAATGGGAAA 
GCTGTGTGCG AGTGCCAGCG GGTGTGCTCG GGCGGCTACG ATCCTGTGTG CGGCAGTGAT 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCGAGTGG CCCGCAGAGG ACCGTGTGAC CGATGTGGGC AGTGCCGGTT TGGATCCTTG 
TGCGAGGTGG AGACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGTGGCT CTGACGGACA CACATATGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACACACCAGA TCAGCCTATA CGTGGCCTCA GCCGGACACT GCCAGACCTG TGGAGAAACA 
GTTTGTACCT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCCCCACCTG GCCCTGTGTG CGGCAGTGAT GGCGTCACCT ACCTCAGTGC CTGTGAGCTC 
CGAGAGGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCGTGCAGG GCCGTGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGG TCTGGGGAAG ACAATGCGTG TGAGCAGGAG 
CTGTGTCGGC AGCATGGTGG TGTCTGGGAT GAGGACTCAG AAGACGGGCC GTGTGTCTGT 
GACTTTAGTT GCCAGAGTGT CCTTAAAAGC CCAGTGTGTG GCTCAGATGG AGTCACCTAT 
AGCACGGAGT GCCATCTGAA GAAGGCCAGA TGTGAAGCGC GGCAAGAGCT GTACGTCGCT 
GCTCAGGGAG CCTGCCGGGG CCCTACCTTG GCTCCACTGC TACCTATGGC CTCCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATGTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCACACG GCTCCTATAG CGGCACTTGT 
GACCCAGTCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GTGACCCTCG GGGTGCTGTA AGAGATGACT GTGAGCAGAT GACTGGATTG 
TGTTCCTGTA GACCTGGTGT GGCTGGTCCC AAGTGTGGGC AGTGTCCAGA TGGTCAAGCC 
CTGGGCCATC TTGGCTGTGA AGCAGATCCC ACAACACCAG TGACTTGTGT GGAAATGCAC 
TGTGAGTTTG GCGCCTCCTG CGTAGAGGAG GCTGGTTTTG CCCAGTGTGT CTGCCCAACT 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGTGGAT CAGATGGTGT CACATACGGC 
AATGAATGCC AGCTGAAGAC CATTGCCTGC CGCCAGCGTC TGGACATCTC CATTCAGAGT 
CTTGGTCCAT GCCGGGAGAG TGTTGCTCCT GGGGTTTCCC CTACATCTGC ATCTATGACC 
ACCCCAAGGC ATATCCTGAG CAGGACACTG GCGTCTCCCC ACAGCAGCCT TCCTCTGTCT 
CCCAGCACTA CTGCCCATGA TTGGCCCACC CCATTACCCA CATCACCTCA GACCGTAGTC 
GGCACCCCCA GGAGCACTGC AGCCACACCC TCTGATGTGG CCAGTCTTGC TACAGCGATC 
TTCAGGGAAT CTGGCAGCAC CAACGGCAGT GGCGATGAGG AGCTCAGTGG CGATGAGGAG 
GCCAGTGGGG GCGGGTCTGG GGGACTTGAG CCCCCGGTGG GCAGCGTTGT GGTGACCCAC 
GGGCCACCCA TCGAGAGGGC TTCCTGTTAC AACTCACCTT TGGGCTGCTG CTCAGATGGC 
AAGACACCCT CACTGGACTC AGAAGGCTCC AACTGTCCAG CTACCAAGGC ATTCCAGGGC 
GTGCTGGAGC TTGAGGGGGT CGAGGGACAG GAACTGTTCT ACACACCAGA GATGGCTGAC 
CCCAAGTCAG AGTTGTTTGG GGAGACTGCA AGGAGCATTG AGAGCACGCT GGACGACCTG 
TTCCGGAATT CGGATGTTAA GAAGGACTTC TGGAGCATCC GCCTACGGGA ACTGGGGCCT 
GGCAAATTAG TCCGTGCCAT TGTGGATGTT CACTTTGACC CCACCACAGC CTTCCAGGCA 
CCAGATGTGG GTCAGGCCTT GCTCCAACAG ATCCAGGTAT CCAGGCCGTG GGCCCTGGCA 
GTGAGGAGGC CTCTGCGGGA GCATGTGCGA TTCTTGGACT TTGACTGGTT TCCCACTTTT 
TTTACGGGAG CTGCAACAGG AACCACAGCT GCTGTGGCCA CAGCCAGAGC CACCACTGTG 
AGCCGACTGT CTGCCTCTTC TGTCACCCCA CGAGTCTACC CCAGTTACAC CAGCCGGCCT 
GTTGGCAGAA CTACGGCACC GCTAACCACT CGCCGGCCAC CAACCACTAC CGCCAGTATT 
GACCGACCTC GGACTCCAGG CCCGCAACGG CCCCCAAAGT CCTGTGATTC CCAGCCTTGC 
CTCCACGGAG GTACCTGCCA GGACCTGGAT TCTGGCAAGG GTTTCAGCTG CAGCTGTACT 
GCAGGCAGGG CTGGCACTGT CTGTGAGAAA GTGCAGCTCC CCTCTGTGCC AGCTTTTAAG 
GGCCACTCCT TCTTGGCCTT CCCCACCCTC CGAGCCTACC ACACGCTGCG TCTGGCACTA 
GAATTCCGGG CGCTGGAGAC AGAGGGACTG CTGCTCTACA ATGGCAATGC ACGTGGCAAA 
GATTTCCTGG CTCTGGCTCT GTTGGATGGT CATGTACAGT TCAGGTTCGA CACGGGCTCA 
GGGCCGGCGG TGCTAACAAG CTTAGTGCCA GTGGAACCGG GACGGTGGCA CCGCCTCGAG 
TTGTCACGGC ATTGGCGGCA GGGCACACTT TCTGTGGATG GCGAGGCTCC TGTTGTAGGT 
GAAAGTCCGA GTGGCACTGA TGGCCTCAAC TTGGACACGA AGCTCTATGT GGGTGGTCTC 
CCAGAAGAAC AAGTTGCCAC GGTGCTTGAT CGGACCTCTG TGGGCATCGG CCTGAAAGGA 
TGCATTCGTA TGTTGGACAT CAACAACCAG CAGCTGGAGC TGAGCGATTG GCAGAGGGCT 
GTGGTTCAAA GCTCTGGTGT GGGGGAATGC GGAGACCATC CCTGCTCACC TAACCCCTGC 
CATGGCGGGG CCCTCTGCCA GGCCCTGGAG GCTGGCGTGT TCCTCTGTCA GTGCCCACCT 
GGCCGCTTTG GCCCAACTTG TGCAGATGAA AAGAACCCCT GCCAACCGAA CCCCTGCCAC 
GGGTCAGCCC CCTGCCATGT GCTTTCCAGG GGTGGGGCCA AGTGTGCGTG CCCCCTGGGA 
CGCAGTGGTT CCTTCTGTGA GACAGTCCTG GAGAATGCTG GCTCCCGGCC CTTCCTGGCT 
GACTTTAATG GCTTCTCCTA CCTGGAACTG AAAGGCTTGC ACACCTTCGA GAGAGACCTA 
GGGGAGAAGA TGGCGCTGGA GATGGTGTTC TTGGCTCGTG GGCCCAGTGG CTTACTCCTC 
TACAATGGGC AGAAGACGGA TGGCAAGGGG GACTTTGTAT CCCTGGCCCT GCATAACCGG 
CACCTAGAGT TCCGCTATGA CCTTGGCAAG GGGGCTGCAA TCATCAGGAG CAAAGAGCCC 
ATAGCCCTGG GCACCTGGGT TAGGGTATTC CTGGAACGAA ATGGCCGCAA GGGTGCCCTT 
CAAGTGGGTG ATGGGCCCCG TGTGCTAGGG GAATCTCCGA AATCCCGCAA GGTCCCGCAC 
ACCATGCTCA ACCTCAAGGA GCCCCTCTAT GTGGGGGGAG CTCCTGACTT CAGCAAGCTG 
GCTCGGGGCG CTGCAGTGGC CTCCGGCTTT GATGGTGCCA TCCAGCTGGT GTCTCTAAGA 
GGCCATCAGC TGCTGACTCA GGAGCATGTG TTGCGGGCAG TAGATGTAGC GCCTTTTGCA 
GGCCACCCTT GTACCCAGGC CGTGGACAAC CCCTGCCTTA ATGGGGGCTC CTGTATCCCG 
AGGGAAGCCA CTTATGAGTG CCTGTGTCCT GGGGGCTTCT CTGGGCTGCA CTGCGAGAAG 
GGGATAGTTG AGAAGTCAGT GGGGGACCTA GAAACACTGG CCTTTGATGG GCGGACCTAC 
ATCGAGTACC TCAATGCTGT GACTGAGAGC GAGCTGACCA ATGAGATCCC AGCCCCCGAA 
ACTCTGGATT CCCGGGCCCT TTTCAGTGAG AAAGCGCTGC AGAGCAACCA CTTTGAGCTG 
AGCTTACGCA CTGAGGCCAC GCAGGGGCTG GTGCTGTGGA TTGGAAAGGT TGGAGAACGT 
GCAGACTACA TGGCTCTGGC CATTGTGGAT GGGCACCTAC AACTGAGCTA TGACCTAGGC 
TCCCAGCCAG TTGTGCTGCG CTCCACTGTG AAGGTCAACA CCAACCGCTG GCTTCGAGTC 
AGGGCTCACA GGGAGCACAG GGAAGGTTCC CTTCAGGTGG GCAATGAAGC CCCTGTGACT 
GGCTCTTCCC CGCTGGGTGC CACACAATTG GACACAGATG GAGCCCTGTG GCTTGGAGGC 
CTACAGAAGC TTCCTGTGGG GCAGGCTCTC CCCAAGGCCT ATGGCACGGG TTTTGTGGGC 
TGTCTGCGGG ACGTGGTAGT GGGCCATCGC CAGCTGCATC TGCTGGAGGA CGCTGTCACC 
AAACCAGAGC TAAGACCCTG CCCCACTCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248479.1
CDS	535..6387
Translation	MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_011250177.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250177.2

ORF Insert Sequence:

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGCAGC CTGGTGCCTC CGTGCTGGTT 
CGGTACTTCA TGATCCCCTG CAACATCTGC TTGATCCTCT TGGCTACTTC TACGTTGGGC 
TTTGCGGTGC TGCTTTTCCT CAGCAACTAC AAACCTGGGA TCCACTTCAC AGCAGCGCCT 
TCTATGCCTC CTGATGTGTG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG GGCCTCCTGT GTGTGCAAGA AGAATGTCTG CCCTGCTATG 
GTAGCTCCTG TGTGTGGCTC AGATGCTTCC ACCTATAGCA ACGAGTGTGA GCTACAGCGT 
GCACAGTGCA ACCAGCAACG GCGCATCCGC CTACTCCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAATGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC AGCCGATGGA 
CAGACCGCCT CGTGTCTGTG TCCTACAACC TGCTTTGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGATG GTGTTGACTA CCCTAGTGAG TGCCAGCTGC TCCGTCATGC CTGTGCCAAC 
CAGGAGCACA TCTTTAAGAA GTTCGATGGT CCTTGTGACC CCTGCCAGGG CAGCATGTCA 
GACCTGAATC ATATTTGCCG GGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCCGCCCA ACACACACCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA GCCCGTGGCC TGCTTCTCCA GAAAGTGCGC 
TCTGGTCAAT GCCAGACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCTGTATGC 
CTGTCCCGCC GCGGCCGTCC CCACTGTTCC TGCGATCGCG TCACCTGTGA TGGGGCTTAC 
AGGCCAGTGT GTGCCCAGGA TGGGCACACG TATGACAATG ACTGTTGGCG CCAACAGGCC 
GAGTGTCGAC AACAGCAGAC CATTCCCCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCA 
TCCCCGTGCC GTGGAGCGCA GTGTGCATTT GGGGCAACAT GCACAGTGAA GAATGGGAAA 
GCTGTGTGCG AGTGCCAGCG GGTGTGCTCG GGCGGCTACG ATCCTGTGTG CGGCAGTGAT 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCGAGTGG CCCGCAGAGG ACCGTGTGAC CGATGTGGGC AGTGCCGGTT TGGATCCTTG 
TGCGAGGTGG AGACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGTGGCT CTGACGGACA CACATATGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACACACCAGA TCAGCCTATA CGTGGCCTCA GCCGGACACT GCCAGACCTG TGGAGAAACA 
GTTTGTACCT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCCCCACCTG GCCCTGTGTG CGGCAGTGAT GGCGTCACCT ACCTCAGTGC CTGTGAGCTC 
CGAGAGGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCGTGCAGG GCCGTGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGG TCTGGGGAAG ACAATGCGTG TGAGCAGGAG 
CTGTGTCGGC AGCATGGTGG TGTCTGGGAT GAGGACTCAG AAGACGGGCC GTGTGTCTGT 
GACTTTAGTT GCCAGAGTGT CCTTAAAAGC CCAGTGTGTG GCTCAGATGG AGTCACCTAT 
AGCACGGAGT GCCATCTGAA GAAGGCCAGA TGTGAAGCGC GGCAAGAGCT GTACGTCGCT 
GCTCAGGGAG CCTGCCGGGG CCCTACCTTG GCTCCACTGC TACCTATGGC CTCCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATGTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCACACG GCTCCTATAG CGGCACTTGT 
GACCCAGTCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAGCCTG GCTTCTGGAA CTTCCGTGGC ATTGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GTGACCCTCG GGGTGCTGTA AGAGATGACT GTGAGCAGAT GACTGGATTG 
TGTTCCTGTA GACCTGGTGT GGCTGGTCCC AAGTGTGGGC AGTGTCCAGA TGGTCAAGCC 
CTGGGCCATC TTGGCTGTGA AGCAGATCCC ACAACACCAG TGACTTGTGT GGAAATGCAC 
TGTGAGTTTG GCGCCTCCTG CGTAGAGGAG GCTGGTTTTG CCCAGTGTGT CTGCCCAACT 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGTGGAT CAGATGGTGT CACATACGGC 
AATGAATGCC AGCTGAAGAC CATTGCCTGC CGCCAGCGTC TGGACATCTC CATTCAGAGT 
CTTGGTCCAT GCCGGGAGAG TGTTGCTCCT GGGGTTTCCC CTACATCTGC ATCTATGACC 
ACCCCAAGGC ATATCCTGAG CAGGACACTG GCGTCTCCCC ACAGCAGCCT TCCTCTGTCT 
CCCAGCACTA CTGCCCATGA TTGGCCCACC CCATTACCCA CATCACCTCA GACCGTAGTC 
GGCACCCCCA GGAGCACTGC AGCCACACCC TCTGATGTGG CCAGTCTTGC TACAGCGATC 
TTCAGGGAAT CTGGCAGCAC CAACGGCAGT GGCGATGAGG AGCTCAGTGG CGATGAGGAG 
GCCAGTGGGG GCGGGTCTGG GGGACTTGAG CCCCCGGTGG GCAGCGTTGT GGTGACCCAC 
GGGCCACCCA TCGAGAGGGC TTCCTGTTAC AACTCACCTT TGGGCTGCTG CTCAGATGGC 
AAGACACCCT CACTGGACTC AGAAGGCTCC AACTGTCCAG CTACCAAGGC ATTCCAGGGC 
GTGCTGGAGC TTGAGGGGGT CGAGGGACAG GAACTGTTCT ACACACCAGA GATGGCTGAC 
CCCAAGTCAG AGTTGTTTGG GGAGACTGCA AGGAGCATTG AGAGCACGCT GGACGACCTG 
TTCCGGAATT CGGATGTTAA GAAGGACTTC TGGAGCATCC GCCTACGGGA ACTGGGGCCT 
GGCAAATTAG TCCGTGCCAT TGTGGATGTT CACTTTGACC CCACCACAGC CTTCCAGGCA 
CCAGATGTGG GTCAGGCCTT GCTCCAACAG ATCCAGGTAT CCAGGCCGTG GGCCCTGGCA 
GTGAGGAGGC CTCTGCGGGA GCATGTGCGA TTCTTGGACT TTGACTGGTT TCCCACTTTT 
TTTACGGGAG CTGCAACAGG AACCACAGCT GCTGTGGCCA CAGCCAGAGC CACCACTGTG 
AGCCGACTGT CTGCCTCTTC TGTCACCCCA CGAGTCTACC CCAGTTACAC CAGCCGGCCT 
GTTGGCAGAA CTACGGCACC GCTAACCACT CGCCGGCCAC CAACCACTAC CGCCAGTATT 
GACCGACCTC GGACTCCAGG CCCGCAACGG CCCCCAAAGT CCTGTGATTC CCAGCCTTGC 
CTCCACGGAG GTACCTGCCA GGACCTGGAT TCTGGCAAGG GTTTCAGCTG CAGCTGTACT 
GCAGGCAGGG CTGGCACTGT CTGTGAGAAA GTGCAGCTCC CCTCTGTGCC AGCTTTTAAG 
GGCCACTCCT TCTTGGCCTT CCCCACCCTC CGAGCCTACC ACACGCTGCG TCTGGCACTA 
GAATTCCGGG CGCTGGAGAC AGAGGGACTG CTGCTCTACA ATGGCAATGC ACGTGGCAAA 
GATTTCCTGG CTCTGGCTCT GTTGGATGGT CATGTACAGT TCAGGTTCGA CACGGGCTCA 
GGGCCGGCGG TGCTAACAAG CTTAGTGCCA GTGGAACCGG GACGGTGGCA CCGCCTCGAG 
TTGTCACGGC ATTGGCGGCA GGGCACACTT TCTGTGGATG GCGAGGCTCC TGTTGTAGGT 
GAAAGTCCGA GTGGCACTGA TGGCCTCAAC TTGGACACGA AGCTCTATGT GGGTGGTCTC 
CCAGAAGAAC AAGTTGCCAC GGTGCTTGAT CGGACCTCTG TGGGCATCGG CCTGAAAGGA 
TGCATTCGTA TGTTGGACAT CAACAACCAG CAGCTGGAGC TGAGCGATTG GCAGAGGGCT 
GTGGTTCAAA GCTCTGGTGT GGGGGAATGC GGAGACCATC CCTGCTCACC TAACCCCTGC 
CATGGCGGGG CCCTCTGCCA GGCCCTGGAG GCTGGCGTGT TCCTCTGTCA GTGCCCACCT 
GGCCGCTTTG GCCCAACTTG TGCAGATGAA AAGAACCCCT GCCAACCGAA CCCCTGCCAC 
GGGTCAGCCC CCTGCCATGT GCTTTCCAGG GGTGGGGCCA AGTGTGCGTG CCCCCTGGGA 
CGCAGTGGTT CCTTCTGTGA GACAGTCCTG GAGAATGCTG GCTCCCGGCC CTTCCTGGCT 
GACTTTAATG GCTTCTCCTA CCTGGAACTG AAAGGCTTGC ACACCTTCGA GAGAGACCTA 
GGGGAGAAGA TGGCGCTGGA GATGGTGTTC TTGGCTCGTG GGCCCAGTGG CTTACTCCTC 
TACAATGGGC AGAAGACGGA TGGCAAGGGG GACTTTGTAT CCCTGGCCCT GCATAACCGG 
CACCTAGAGT TCCGCTATGA CCTTGGCAAG GGGGCTGCAA TCATCAGGAG CAAAGAGCCC 
ATAGCCCTGG GCACCTGGGT TAGGGTATTC CTGGAACGAA ATGGCCGCAA GGGTGCCCTT 
CAAGTGGGTG ATGGGCCCCG TGTGCTAGGG GAATCTCCGA AATCCCGCAA GGTCCCGCAC 
ACCATGCTCA ACCTCAAGGA GCCCCTCTAT GTGGGGGGAG CTCCTGACTT CAGCAAGCTG 
GCTCGGGGCG CTGCAGTGGC CTCCGGCTTT GATGGTGCCA TCCAGCTGGT GTCTCTAAGA 
GGCCATCAGC TGCTGACTCA GGAGCATGTG TTGCGGGCAG TAGATGTAGC GCCTTTTGCA 
GGCCACCCTT GTACCCAGGC CGTGGACAAC CCCTGCCTTA ATGGGGGCTC CTGTATCCCG 
AGGGAAGCCA CTTATGAGTG CCTGTGTCCT GGGGGCTTCT CTGGGCTGCA CTGCGAGAAG 
GGGATAGTTG AGAAGTCAGT GGGGGACCTA GAAACACTGG CCTTTGATGG GCGGACCTAC 
ATCGAGTACC TCAATGCTGT GACTGAGAGC GAGCTGACCA ATGAGATCCC AGCCCCCGAA 
ACTCTGGATT CCCGGGCCCT TTTCAGTGAG AAAGCGCTGC AGAGCAACCA CTTTGAGCTG 
AGCTTACGCA CTGAGGCCAC GCAGGGGCTG GTGCTGTGGA TTGGAAAGGT TGGAGAACGT 
GCAGACTACA TGGCTCTGGC CATTGTGGAT GGGCACCTAC AACTGAGCTA TGACCTAGGC 
TCCCAGCCAG TTGTGCTGCG CTCCACTGTG AAGGTCAACA CCAACCGCTG GCTTCGAGTC 
AGGGCTCACA GGGAGCACAG GGAAGGTTCC CTTCAGGTGG GCAATGAAGC CCCTGTGACT 
GGCTCTTCCC CGCTGGGTGC CACACAATTG GACACAGATG GAGCCCTGTG GCTTGGAGGC 
CTACAGAAGC TTCCTGTGGG GCAGGCTCTC CCCAAGGCCT ATGGCACGGG TTTTGTGGGC 
TGTCTGCGGG ACGTGGTAGT GGGCCATCGC CAGCTGCATC TGCTGGAGGA CGCTGTCACC 
AAACCAGAGC TAAGACCCTG CCCCACTCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_011250178.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248480.1 Mus musculus agrin (Agrn), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu67086
Clone ID Related Accession (Same CDS sequence)	XM_011250178.1
Accession Version	XM_011250178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGTA CCCTTGGGCG GGAAATCCGA GTGGCCCGCA GAGGACCGTG TGACCGATGT 
GGGCAGTGCC GGTTTGGATC CTTGTGCGAG GTGGAGACTG GACGCTGTGT GTGCCCCTCT 
GAGTGTGTGG AGTCAGCCCA GCCCGTATGT GGCTCTGACG GACACACATA TGCTAGTGAA 
TGTGAGCTGC ATGTCCACGC CTGTACACAC CAGATCAGCC TATACGTGGC CTCAGCCGGA 
CACTGCCAGA CCTGTGGAGA AACAGTTTGT ACCTTTGGGG CTGTGTGCTC AGCTGGACAG 
TGTGTATGTC CCCGTTGTGA GCACCCCCCA CCTGGCCCTG TGTGCGGCAG TGATGGCGTC 
ACCTACCTCA GTGCCTGTGA GCTCCGAGAG GCTGCCTGTC AGCAGCAGGT ACAAATTGAG 
GAGGCCCGTG CAGGGCCGTG TGAGCCGGCT GAGTGTGGCT CAGGGGGCTC TGGGTCTGGG 
GAAGACAATG CGTGTGAGCA GGAGCTGTGT CGGCAGCATG GTGGTGTCTG GGATGAGGAC 
TCAGAAGACG GGCCGTGTGT CTGTGACTTT AGTTGCCAGA GTGTCCTTAA AAGCCCAGTG 
TGTGGCTCAG ATGGAGTCAC CTATAGCACG GAGTGCCATC TGAAGAAGGC CAGATGTGAA 
GCGCGGCAAG AGCTGTACGT CGCTGCTCAG GGAGCCTGCC GGGGCCCTAC CTTGGCTCCA 
CTGCTACCTA TGGCCTCCCC ACACTGTGCC CAAACCCCCT ATGGCTGCTG CCAGGACAAT 
GTCACTGCTG CCCAGGGTGT GGGCTTGGCT GGCTGTCCCA GCACCTGCCA TTGCAACCCA 
CACGGCTCCT ATAGCGGCAC TTGTGACCCA GTCACAGGGC AGTGCTCCTG CCGACCAGGT 
GTAGGAGGCC TCAGGTGTGA TCGCTGTGAG CCTGGCTTCT GGAACTTCCG TGGCATTGTC 
ACCGATGGAC ATAGTGGTTG CACTCCCTGC AGCTGTGACC CTCGGGGTGC TGTAAGAGAT 
GACTGTGAGC AGATGACTGG ATTGTGTTCC TGTAGACCTG GTGTGGCTGG TCCCAAGTGT 
GGGCAGTGTC CAGATGGTCA AGCCCTGGGC CATCTTGGCT GTGAAGCAGA TCCCACAACA 
CCAGTGACTT GTGTGGAAAT GCACTGTGAG TTTGGCGCCT CCTGCGTAGA GGAGGCTGGT 
TTTGCCCAGT GTGTCTGCCC AACTCTCACA TGTCCAGAGG CTAACTCTAC CAAGGTCTGT 
GGATCAGATG GTGTCACATA CGGCAATGAA TGCCAGCTGA AGACCATTGC CTGCCGCCAG 
CGTCTGGACA TCTCCATTCA GAGTCTTGGT CCATGCCGGG AGAGTGTTGC TCCTGGGGTT 
TCCCCTACAT CTGCATCTAT GACCACCCCA AGGCATATCC TGAGCAGGAC ACTGGCGTCT 
CCCCACAGCA GCCTTCCTCT GTCTCCCAGC ACTACTGCCC ATGATTGGCC CACCCCATTA 
CCCACATCAC CTCAGACCGT AGTCGGCACC CCCAGGAGCA CTGCAGCCAC ACCCTCTGAT 
GTGGCCAGTC TTGCTACAGC GATCTTCAGG GAATCTGGCA GCACCAACGG CAGTGGCGAT 
GAGGAGCTCA GTGGCGATGA GGAGGCCAGT GGGGGCGGGT CTGGGGGACT TGAGCCCCCG 
GTGGGCAGCG TTGTGGTGAC CCACGGGCCA CCCATCGAGA GGGCTTCCTG TTACAACTCA 
CCTTTGGGCT GCTGCTCAGA TGGCAAGACA CCCTCACTGG ACTCAGAAGG CTCCAACTGT 
CCAGCTACCA AGGCATTCCA GGGCGTGCTG GAGCTTGAGG GGGTCGAGGG ACAGGAACTG 
TTCTACACAC CAGAGATGGC TGACCCCAAG TCAGAGTTGT TTGGGGAGAC TGCAAGGAGC 
ATTGAGAGCA CGCTGGACGA CCTGTTCCGG AATTCGGATG TTAAGAAGGA CTTCTGGAGC 
ATCCGCCTAC GGGAACTGGG GCCTGGCAAA TTAGTCCGTG CCATTGTGGA TGTTCACTTT 
GACCCCACCA CAGCCTTCCA GGCACCAGAT GTGGGTCAGG CCTTGCTCCA ACAGATCCAG 
GTATCCAGGC CGTGGGCCCT GGCAGTGAGG AGGCCTCTGC GGGAGCATGT GCGATTCTTG 
GACTTTGACT GGTTTCCCAC TTTTTTTACG GGAGCTGCAA CAGGAACCAC AGCTGCTGTG 
GCCACAGCCA GAGCCACCAC TGTGAGCCGA CTGTCTGCCT CTTCTGTCAC CCCACGAGTC 
TACCCCAGTT ACACCAGCCG GCCTGTTGGC AGAACTACGG CACCGCTAAC CACTCGCCGG 
CCACCAACCA CTACCGCCAG TATTGACCGA CCTCGGACTC CAGGCCCGCA ACGGCCCCCA 
AAGTCCTGTG ATTCCCAGCC TTGCCTCCAC GGAGGTACCT GCCAGGACCT GGATTCTGGC 
AAGGGTTTCA GCTGCAGCTG TACTGCAGGC AGGGCTGGCA CTGTCTGTGA GAAAGTGCAG 
CTCCCCTCTG TGCCAGCTTT TAAGGGCCAC TCCTTCTTGG CCTTCCCCAC CCTCCGAGCC 
TACCACACGC TGCGTCTGGC ACTAGAATTC CGGGCGCTGG AGACAGAGGG ACTGCTGCTC 
TACAATGGCA ATGCACGTGG CAAAGATTTC CTGGCTCTGG CTCTGTTGGA TGGTCATGTA 
CAGTTCAGGT TCGACACGGG CTCAGGGCCG GCGGTGCTAA CAAGCTTAGT GCCAGTGGAA 
CCGGGACGGT GGCACCGCCT CGAGTTGTCA CGGCATTGGC GGCAGGGCAC ACTTTCTGTG 
GATGGCGAGG CTCCTGTTGT AGGTGAAAGT CCGAGTGGCA CTGATGGCCT CAACTTGGAC 
ACGAAGCTCT ATGTGGGTGG TCTCCCAGAA GAACAAGTTG CCACGGTGCT TGATCGGACC 
TCTGTGGGCA TCGGCCTGAA AGGATGCATT CGTATGTTGG ACATCAACAA CCAGCAGCTG 
GAGCTGAGCG ATTGGCAGAG GGCTGTGGTT CAAAGCTCTG GTGTGGGGGA ATGCGGAGAC 
CATCCCTGCT CACCTAACCC CTGCCATGGC GGGGCCCTCT GCCAGGCCCT GGAGGCTGGC 
GTGTTCCTCT GTCAGTGCCC ACCTGGCCGC TTTGGCCCAA CTTGTGCAGA TGAAAAGAAC 
CCCTGCCAAC CGAACCCCTG CCACGGGTCA GCCCCCTGCC ATGTGCTTTC CAGGGGTGGG 
GCCAAGTGTG CGTGCCCCCT GGGACGCAGT GGTTCCTTCT GTGAGACAGT CCTGGAGAAT 
GCTGGCTCCC GGCCCTTCCT GGCTGACTTT AATGGCTTCT CCTACCTGGA ACTGAAAGGC 
TTGCACACCT TCGAGAGAGA CCTAGGGGAG AAGATGGCGC TGGAGATGGT GTTCTTGGCT 
CGTGGGCCCA GTGGCTTACT CCTCTACAAT GGGCAGAAGA CGGATGGCAA GGGGGACTTT 
GTATCCCTGG CCCTGCATAA CCGGCACCTA GAGTTCCGCT ATGACCTTGG CAAGGGGGCT 
GCAATCATCA GGAGCAAAGA GCCCATAGCC CTGGGCACCT GGGTTAGGGT ATTCCTGGAA 
CGAAATGGCC GCAAGGGTGC CCTTCAAGTG GGTGATGGGC CCCGTGTGCT AGGGGAATCT 
CCGAAATCCC GCAAGGTCCC GCACACCATG CTCAACCTCA AGGAGCCCCT CTATGTGGGG 
GGAGCTCCTG ACTTCAGCAA GCTGGCTCGG GGCGCTGCAG TGGCCTCCGG CTTTGATGGT 
GCCATCCAGC TGGTGTCTCT AAGAGGCCAT CAGCTGCTGA CTCAGGAGCA TGTGTTGCGG 
GCAGTAGATG TAGCGCCTTT TGCAGGCCAC CCTTGTACCC AGGCCGTGGA CAACCCCTGC 
CTTAATGGGG GCTCCTGTAT CCCGAGGGAA GCCACTTATG AGTGCCTGTG TCCTGGGGGC 
TTCTCTGGGC TGCACTGCGA GAAGGGGATA GTTGAGAAGT CAGTGGGGGA CCTAGAAACA 
CTGGCCTTTG ATGGGCGGAC CTACATCGAG TACCTCAATG CTGTGACTGA GAGCGAGCTG 
ACCAATGAGA TCCCAGCCCC CGAAACTCTG GATTCCCGGG CCCTTTTCAG TGAGAAAGCG 
CTGCAGAGCA ACCACTTTGA GCTGAGCTTA CGCACTGAGG CCACGCAGGG GCTGGTGCTG 
TGGATTGGAA AGGTTGGAGA ACGTGCAGAC TACATGGCTC TGGCCATTGT GGATGGGCAC 
CTACAACTGA GCTATGACCT AGGCTCCCAG CCAGTTGTGC TGCGCTCCAC TGTGAAGGTC 
AACACCAACC GCTGGCTTCG AGTCAGGGCT CACAGGGAGC ACAGGGAAGG TTCCCTTCAG 
GTGGGCAATG AAGCCCCTGT GACTGGCTCT TCCCCGCTGG GTGCCACACA ATTGGACACA 
GATGGAGCCC TGTGGCTTGG AGGCCTACAG AAGCTTCCTG TGGGGCAGGC TCTCCCCAAG 
GCCTATGGCA CGGGTTTTGT GGGCTGTCTG CGGGACGTGG TAGTGGGCCA TCGCCAGCTG 
CATCTGCTGG AGGACGCTGT CACCAAACCA GAGCTAAGAC CCTGCCCCAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248480.1
CDS	232..4848
Translation	MACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_011250178.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250178.1

ORF Insert Sequence:

ATGGCCTGTA CCCTTGGGCG GGAAATCCGA GTGGCCCGCA GAGGACCGTG TGACCGATGT 
GGGCAGTGCC GGTTTGGATC CTTGTGCGAG GTGGAGACTG GACGCTGTGT GTGCCCCTCT 
GAGTGTGTGG AGTCAGCCCA GCCCGTATGT GGCTCTGACG GACACACATA TGCTAGTGAA 
TGTGAGCTGC ATGTCCACGC CTGTACACAC CAGATCAGCC TATACGTGGC CTCAGCCGGA 
CACTGCCAGA CCTGTGGAGA AACAGTTTGT ACCTTTGGGG CTGTGTGCTC AGCTGGACAG 
TGTGTATGTC CCCGTTGTGA GCACCCCCCA CCTGGCCCTG TGTGCGGCAG TGATGGCGTC 
ACCTACCTCA GTGCCTGTGA GCTCCGAGAG GCTGCCTGTC AGCAGCAGGT ACAAATTGAG 
GAGGCCCGTG CAGGGCCGTG TGAGCCGGCT GAGTGTGGCT CAGGGGGCTC TGGGTCTGGG 
GAAGACAATG CGTGTGAGCA GGAGCTGTGT CGGCAGCATG GTGGTGTCTG GGATGAGGAC 
TCAGAAGACG GGCCGTGTGT CTGTGACTTT AGTTGCCAGA GTGTCCTTAA AAGCCCAGTG 
TGTGGCTCAG ATGGAGTCAC CTATAGCACG GAGTGCCATC TGAAGAAGGC CAGATGTGAA 
GCGCGGCAAG AGCTGTACGT CGCTGCTCAG GGAGCCTGCC GGGGCCCTAC CTTGGCTCCA 
CTGCTACCTA TGGCCTCCCC ACACTGTGCC CAAACCCCCT ATGGCTGCTG CCAGGACAAT 
GTCACTGCTG CCCAGGGTGT GGGCTTGGCT GGCTGTCCCA GCACCTGCCA TTGCAACCCA 
CACGGCTCCT ATAGCGGCAC TTGTGACCCA GTCACAGGGC AGTGCTCCTG CCGACCAGGT 
GTAGGAGGCC TCAGGTGTGA TCGCTGTGAG CCTGGCTTCT GGAACTTCCG TGGCATTGTC 
ACCGATGGAC ATAGTGGTTG CACTCCCTGC AGCTGTGACC CTCGGGGTGC TGTAAGAGAT 
GACTGTGAGC AGATGACTGG ATTGTGTTCC TGTAGACCTG GTGTGGCTGG TCCCAAGTGT 
GGGCAGTGTC CAGATGGTCA AGCCCTGGGC CATCTTGGCT GTGAAGCAGA TCCCACAACA 
CCAGTGACTT GTGTGGAAAT GCACTGTGAG TTTGGCGCCT CCTGCGTAGA GGAGGCTGGT 
TTTGCCCAGT GTGTCTGCCC AACTCTCACA TGTCCAGAGG CTAACTCTAC CAAGGTCTGT 
GGATCAGATG GTGTCACATA CGGCAATGAA TGCCAGCTGA AGACCATTGC CTGCCGCCAG 
CGTCTGGACA TCTCCATTCA GAGTCTTGGT CCATGCCGGG AGAGTGTTGC TCCTGGGGTT 
TCCCCTACAT CTGCATCTAT GACCACCCCA AGGCATATCC TGAGCAGGAC ACTGGCGTCT 
CCCCACAGCA GCCTTCCTCT GTCTCCCAGC ACTACTGCCC ATGATTGGCC CACCCCATTA 
CCCACATCAC CTCAGACCGT AGTCGGCACC CCCAGGAGCA CTGCAGCCAC ACCCTCTGAT 
GTGGCCAGTC TTGCTACAGC GATCTTCAGG GAATCTGGCA GCACCAACGG CAGTGGCGAT 
GAGGAGCTCA GTGGCGATGA GGAGGCCAGT GGGGGCGGGT CTGGGGGACT TGAGCCCCCG 
GTGGGCAGCG TTGTGGTGAC CCACGGGCCA CCCATCGAGA GGGCTTCCTG TTACAACTCA 
CCTTTGGGCT GCTGCTCAGA TGGCAAGACA CCCTCACTGG ACTCAGAAGG CTCCAACTGT 
CCAGCTACCA AGGCATTCCA GGGCGTGCTG GAGCTTGAGG GGGTCGAGGG ACAGGAACTG 
TTCTACACAC CAGAGATGGC TGACCCCAAG TCAGAGTTGT TTGGGGAGAC TGCAAGGAGC 
ATTGAGAGCA CGCTGGACGA CCTGTTCCGG AATTCGGATG TTAAGAAGGA CTTCTGGAGC 
ATCCGCCTAC GGGAACTGGG GCCTGGCAAA TTAGTCCGTG CCATTGTGGA TGTTCACTTT 
GACCCCACCA CAGCCTTCCA GGCACCAGAT GTGGGTCAGG CCTTGCTCCA ACAGATCCAG 
GTATCCAGGC CGTGGGCCCT GGCAGTGAGG AGGCCTCTGC GGGAGCATGT GCGATTCTTG 
GACTTTGACT GGTTTCCCAC TTTTTTTACG GGAGCTGCAA CAGGAACCAC AGCTGCTGTG 
GCCACAGCCA GAGCCACCAC TGTGAGCCGA CTGTCTGCCT CTTCTGTCAC CCCACGAGTC 
TACCCCAGTT ACACCAGCCG GCCTGTTGGC AGAACTACGG CACCGCTAAC CACTCGCCGG 
CCACCAACCA CTACCGCCAG TATTGACCGA CCTCGGACTC CAGGCCCGCA ACGGCCCCCA 
AAGTCCTGTG ATTCCCAGCC TTGCCTCCAC GGAGGTACCT GCCAGGACCT GGATTCTGGC 
AAGGGTTTCA GCTGCAGCTG TACTGCAGGC AGGGCTGGCA CTGTCTGTGA GAAAGTGCAG 
CTCCCCTCTG TGCCAGCTTT TAAGGGCCAC TCCTTCTTGG CCTTCCCCAC CCTCCGAGCC 
TACCACACGC TGCGTCTGGC ACTAGAATTC CGGGCGCTGG AGACAGAGGG ACTGCTGCTC 
TACAATGGCA ATGCACGTGG CAAAGATTTC CTGGCTCTGG CTCTGTTGGA TGGTCATGTA 
CAGTTCAGGT TCGACACGGG CTCAGGGCCG GCGGTGCTAA CAAGCTTAGT GCCAGTGGAA 
CCGGGACGGT GGCACCGCCT CGAGTTGTCA CGGCATTGGC GGCAGGGCAC ACTTTCTGTG 
GATGGCGAGG CTCCTGTTGT AGGTGAAAGT CCGAGTGGCA CTGATGGCCT CAACTTGGAC 
ACGAAGCTCT ATGTGGGTGG TCTCCCAGAA GAACAAGTTG CCACGGTGCT TGATCGGACC 
TCTGTGGGCA TCGGCCTGAA AGGATGCATT CGTATGTTGG ACATCAACAA CCAGCAGCTG 
GAGCTGAGCG ATTGGCAGAG GGCTGTGGTT CAAAGCTCTG GTGTGGGGGA ATGCGGAGAC 
CATCCCTGCT CACCTAACCC CTGCCATGGC GGGGCCCTCT GCCAGGCCCT GGAGGCTGGC 
GTGTTCCTCT GTCAGTGCCC ACCTGGCCGC TTTGGCCCAA CTTGTGCAGA TGAAAAGAAC 
CCCTGCCAAC CGAACCCCTG CCACGGGTCA GCCCCCTGCC ATGTGCTTTC CAGGGGTGGG 
GCCAAGTGTG CGTGCCCCCT GGGACGCAGT GGTTCCTTCT GTGAGACAGT CCTGGAGAAT 
GCTGGCTCCC GGCCCTTCCT GGCTGACTTT AATGGCTTCT CCTACCTGGA ACTGAAAGGC 
TTGCACACCT TCGAGAGAGA CCTAGGGGAG AAGATGGCGC TGGAGATGGT GTTCTTGGCT 
CGTGGGCCCA GTGGCTTACT CCTCTACAAT GGGCAGAAGA CGGATGGCAA GGGGGACTTT 
GTATCCCTGG CCCTGCATAA CCGGCACCTA GAGTTCCGCT ATGACCTTGG CAAGGGGGCT 
GCAATCATCA GGAGCAAAGA GCCCATAGCC CTGGGCACCT GGGTTAGGGT ATTCCTGGAA 
CGAAATGGCC GCAAGGGTGC CCTTCAAGTG GGTGATGGGC CCCGTGTGCT AGGGGAATCT 
CCGAAATCCC GCAAGGTCCC GCACACCATG CTCAACCTCA AGGAGCCCCT CTATGTGGGG 
GGAGCTCCTG ACTTCAGCAA GCTGGCTCGG GGCGCTGCAG TGGCCTCCGG CTTTGATGGT 
GCCATCCAGC TGGTGTCTCT AAGAGGCCAT CAGCTGCTGA CTCAGGAGCA TGTGTTGCGG 
GCAGTAGATG TAGCGCCTTT TGCAGGCCAC CCTTGTACCC AGGCCGTGGA CAACCCCTGC 
CTTAATGGGG GCTCCTGTAT CCCGAGGGAA GCCACTTATG AGTGCCTGTG TCCTGGGGGC 
TTCTCTGGGC TGCACTGCGA GAAGGGGATA GTTGAGAAGT CAGTGGGGGA CCTAGAAACA 
CTGGCCTTTG ATGGGCGGAC CTACATCGAG TACCTCAATG CTGTGACTGA GAGCGAGCTG 
ACCAATGAGA TCCCAGCCCC CGAAACTCTG GATTCCCGGG CCCTTTTCAG TGAGAAAGCG 
CTGCAGAGCA ACCACTTTGA GCTGAGCTTA CGCACTGAGG CCACGCAGGG GCTGGTGCTG 
TGGATTGGAA AGGTTGGAGA ACGTGCAGAC TACATGGCTC TGGCCATTGT GGATGGGCAC 
CTACAACTGA GCTATGACCT AGGCTCCCAG CCAGTTGTGC TGCGCTCCAC TGTGAAGGTC 
AACACCAACC GCTGGCTTCG AGTCAGGGCT CACAGGGAGC ACAGGGAAGG TTCCCTTCAG 
GTGGGCAATG AAGCCCCTGT GACTGGCTCT TCCCCGCTGG GTGCCACACA ATTGGACACA 
GATGGAGCCC TGTGGCTTGG AGGCCTACAG AAGCTTCCTG TGGGGCAGGC TCTCCCCAAG 
GCCTATGGCA CGGGTTTTGT GGGCTGTCTG CGGGACGTGG TAGTGGGCCA TCGCCAGCTG 
CATCTGCTGG AGGACGCTGT CACCAAACCA GAGCTAAGAC CCTGCCCCAC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_017319921.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175410.1 Mus musculus agrin (Agrn), transcript variant X8, mRNA.

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CloneID	OMu80818
Clone ID Related Accession (Same CDS sequence)	XM_017319921.1
Accession Version	XM_017319921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	agrin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGA CCCCCACCCG CACACCCACA GGGTCCCGGG ACCCCTGTGC CAATGTGACC 
TGCAGTTTCG GTAGTACCTG TGTACCTTCA GCCGATGGAC AGACCGCCTC GTGTCTGTGT 
CCTACAACCT GCTTTGGGGC CCCTGATGGC ACAGTGTGTG GCAGTGATGG TGTTGACTAC 
CCTAGTGAGT GCCAGCTGCT CCGTCATGCC TGTGCCAACC AGGAGCACAT CTTTAAGAAG 
TTCGATGGTC CTTGTGACCC CTGCCAGGGC AGCATGTCAG ACCTGAATCA TATTTGCCGG 
GTGAACCCAC GTACACGGCA CCCAGAAATG CTTCTGCGGC CTGAGAACTG CCCCGCCCAA 
CACACACCTA TCTGTGGAGA TGATGGGGTC ACCTATGAAA ACGACTGTGT CATGAGCCGT 
ATAGGTGCAG CCCGTGGCCT GCTTCTCCAG AAAGTGCGCT CTGGTCAATG CCAGACTCGA 
GACCAGTGCC CGGAGACCTG CCAGTTTAAC TCTGTATGCC TGTCCCGCCG CGGCCGTCCC 
CACTGTTCCT GCGATCGCGT CACCTGTGAT GGGGCTTACA GGCCAGTGTG TGCCCAGGAT 
GGGCACACGT ATGACAATGA CTGTTGGCGC CAACAGGCCG AGTGTCGACA ACAGCAGACC 
ATTCCCCCCA AGCACCAGGG CCCGTGTGAC CAGACCCCAT CCCCGTGCCG TGGAGCGCAG 
TGTGCATTTG GGGCAACATG CACAGTGAAG AATGGGAAAG CTGTGTGCGA GTGCCAGCGG 
GTGTGCTCGG GCGGCTACGA TCCTGTGTGC GGCAGTGATG GTGTCACTTA CGGCAGTGTG 
TGCGAGCTGG AATCCATGGC CTGTACCCTT GGGCGGGAAA TCCGAGTGGC CCGCAGAGGA 
CCGTGTGACC GATGTGGGCA GTGCCGGTTT GGATCCTTGT GCGAGGTGGA GACTGGACGC 
TGTGTGTGCC CCTCTGAGTG TGTGGAGTCA GCCCAGCCCG TATGTGGCTC TGACGGACAC 
ACATATGCTA GTGAATGTGA GCTGCATGTC CACGCCTGTA CACACCAGAT CAGCCTATAC 
GTGGCCTCAG CCGGACACTG CCAGACCTGT GGAGAAACAG TTTGTACCTT TGGGGCTGTG 
TGCTCAGCTG GACAGTGTGT ATGTCCCCGT TGTGAGCACC CCCCACCTGG CCCTGTGTGC 
GGCAGTGATG GCGTCACCTA CCTCAGTGCC TGTGAGCTCC GAGAGGCTGC CTGTCAGCAG 
CAGGTACAAA TTGAGGAGGC CCGTGCAGGG CCGTGTGAGC CGGCTGAGTG TGGCTCAGGG 
GGCTCTGGGT CTGGGGAAGA CAATGCGTGT GAGCAGGAGC TGTGTCGGCA GCATGGTGGT 
GTCTGGGATG AGGACTCAGA AGACGGGCCG TGTGTCTGTG ACTTTAGTTG CCAGAGTGTC 
CTTAAAAGCC CAGTGTGTGG CTCAGATGGA GTCACCTATA GCACGGAGTG CCATCTGAAG 
AAGGCCAGAT GTGAAGCGCG GCAAGAGCTG TACGTCGCTG CTCAGGGAGC CTGCCGGGGC 
CCTACCTTGG CTCCACTGCT ACCTATGGCC TCCCCACACT GTGCCCAAAC CCCCTATGGC 
TGCTGCCAGG ACAATGTCAC TGCTGCCCAG GGTGTGGGCT TGGCTGGCTG TCCCAGCACC 
TGCCATTGCA ACCCACACGG CTCCTATAGC GGCACTTGTG ACCCAGTCAC AGGGCAGTGC 
TCCTGCCGAC CAGGTGTAGG AGGCCTCAGG TGTGATCGCT GTGAGCCTGG CTTCTGGAAC 
TTCCGTGGCA TTGTCACCGA TGGACATAGT GGTTGCACTC CCTGCAGCTG TGACCCTCGG 
GGTGCTGTAA GAGATGACTG TGAGCAGATG ACTGGATTGT GTTCCTGTAG ACCTGGTGTG 
GCTGGTCCCA AGTGTGGGCA GTGTCCAGAT GGTCAAGCCC TGGGCCATCT TGGCTGTGAA 
GCAGATCCCA CAACACCAGT GACTTGTGTG GAAATGCACT GTGAGTTTGG CGCCTCCTGC 
GTAGAGGAGG CTGGTTTTGC CCAGTGTGTC TGCCCAACTC TCACATGTCC AGAGGCTAAC 
TCTACCAAGG TCTGTGGATC AGATGGTGTC ACATACGGCA ATGAATGCCA GCTGAAGACC 
ATTGCCTGCC GCCAGCGTCT GGACATCTCC ATTCAGAGTC TTGGTCCATG CCGGGAGAGT 
GTTGCTCCTG GGGTTTCCCC TACATCTGCA TCTATGACCA CCCCAAGGCA TATCCTGAGC 
AGGACACTGG CGTCTCCCCA CAGCAGCCTT CCTCTGTCTC CCAGCACTAC TGCCCATGAT 
TGGCCCACCC CATTACCCAC ATCACCTCAG ACCGTAGTCG GCACCCCCAG GAGCACTGCA 
GCCACACCCT CTGATGTGGC CAGTCTTGCT ACAGCGATCT TCAGGGAATC TGGCAGCACC 
AACGGCAGTG GCGATGAGGA GCTCAGTGGC GATGAGGAGG CCAGTGGGGG CGGGTCTGGG 
GGACTTGAGC CCCCGGTGGG CAGCGTTGTG GTGACCCACG GGCCACCCAT CGAGAGGGCT 
TCCTGTTACA ACTCACCTTT GGGCTGCTGC TCAGATGGCA AGACACCCTC ACTGGACTCA 
GAAGGCTCCA ACTGTCCAGC TACCAAGGCA TTCCAGGGCG TGCTGGAGCT TGAGGGGGTC 
GAGGGACAGG AACTGTTCTA CACACCAGAG ATGGCTGACC CCAAGTCAGA GTTGTTTGGG 
GAGACTGCAA GGAGCATTGA GAGCACGCTG GACGACCTGT TCCGGAATTC GGATGTTAAG 
AAGGACTTCT GGAGCATCCG CCTACGGGAA CTGGGGCCTG GCAAATTAGT CCGTGCCATT 
GTGGATGTTC ACTTTGACCC CACCACAGCC TTCCAGGCAC CAGATGTGGG TCAGGCCTTG 
CTCCAACAGA TCCAGGTATC CAGGCCGTGG GCCCTGGCAG TGAGGAGGCC TCTGCGGGAG 
CATGTGCGAT TCTTGGACTT TGACTGGTTT CCCACTTTTT TTACGGGAGC TGCAACAGGA 
ACCACAGCTG CTGTGGCCAC AGCCAGAGCC ACCACTGTGA GCCGACTGTC TGCCTCTTCT 
GTCACCCCAC GAGTCTACCC CAGTTACACC AGCCGGCCTG TTGGCAGAAC TACGGCACCG 
CTAACCACTC GCCGGCCACC AACCACTACC GCCAGTATTG ACCGACCTCG GACTCCAGGC 
CCGCAACGGC CCCCAAAGTC CTGTGATTCC CAGCCTTGCC TCCACGGAGG TACCTGCCAG 
GACCTGGATT CTGGCAAGGG TTTCAGCTGC AGCTGTACTG CAGGCAGGGC TGGCACTGTC 
TGTGAGAAAG TGCAGCTCCC CTCTGTGCCA GCTTTTAAGG GCCACTCCTT CTTGGCCTTC 
CCCACCCTCC GAGCCTACCA CACGCTGCGT CTGGCACTAG AATTCCGGGC GCTGGAGACA 
GAGGGACTGC TGCTCTACAA TGGCAATGCA CGTGGCAAAG ATTTCCTGGC TCTGGCTCTG 
TTGGATGGTC ATGTACAGTT CAGGTTCGAC ACGGGCTCAG GGCCGGCGGT GCTAACAAGC 
TTAGTGCCAG TGGAACCGGG ACGGTGGCAC CGCCTCGAGT TGTCACGGCA TTGGCGGCAG 
GGCACACTTT CTGTGGATGG CGAGGCTCCT GTTGTAGGTG AAAGTCCGAG TGGCACTGAT 
GGCCTCAACT TGGACACGAA GCTCTATGTG GGTGGTCTCC CAGAAGAACA AGTTGCCACG 
GTGCTTGATC GGACCTCTGT GGGCATCGGC CTGAAAGGAT GCATTCGTAT GTTGGACATC 
AACAACCAGC AGCTGGAGCT GAGCGATTGG CAGAGGGCTG TGGTTCAAAG CTCTGGTGTG 
GGGGAATGCG GAGACCATCC CTGCTCACCT AACCCCTGCC ATGGCGGGGC CCTCTGCCAG 
GCCCTGGAGG CTGGCGTGTT CCTCTGTCAG TGCCCACCTG GCCGCTTTGG CCCAACTTGT 
GCAGATGAAA AGAACCCCTG CCAACCGAAC CCCTGCCACG GGTCAGCCCC CTGCCATGTG 
CTTTCCAGGG GTGGGGCCAA GTGTGCGTGC CCCCTGGGAC GCAGTGGTTC CTTCTGTGAG 
ACAGTCCTGG AGAATGCTGG CTCCCGGCCC TTCCTGGCTG ACTTTAATGG CTTCTCCTAC 
CTGGAACTGA AAGGCTTGCA CACCTTCGAG AGAGACCTAG GGGAGAAGAT GGCGCTGGAG 
ATGGTGTTCT TGGCTCGTGG GCCCAGTGGC TTACTCCTCT ACAATGGGCA GAAGACGGAT 
GGCAAGGGGG ACTTTGTATC CCTGGCCCTG CATAACCGGC ACCTAGAGTT CCGCTATGAC 
CTTGGCAAGG GGGCTGCAAT CATCAGGAGC AAAGAGCCCA TAGCCCTGGG CACCTGGGTT 
AGGGTATTCC TGGAACGAAA TGGCCGCAAG GGTGCCCTTC AAGTGGGTGA TGGGCCCCGT 
GTGCTAGGGG AATCTCCGAA ATCCCGCAAG GTCCCGCACA CCATGCTCAA CCTCAAGGAG 
CCCCTCTATG TGGGGGGAGC TCCTGACTTC AGCAAGCTGG CTCGGGGCGC TGCAGTGGCC 
TCCGGCTTTG ATGGTGCCAT CCAGCTGGTG TCTCTAAGAG GCCATCAGCT GCTGACTCAG 
GAGCATGTGT TGCGGGCAGT AGATGTAGCG CCTTTTGCAG GCCACCCTTG TACCCAGGCC 
GTGGACAACC CCTGCCTTAA TGGGGGCTCC TGTATCCCGA GGGAAGCCAC TTATGAGTGC 
CTGTGTCCTG GGGGCTTCTC TGGGCTGCAC TGCGAGAAGG GGATAGTTGA GAAGTCAGTG 
GGGGACCTAG AAACACTGGC CTTTGATGGG CGGACCTACA TCGAGTACCT CAATGCTGTG 
ACTGAGAGCG AGCTGACCAA TGAGATCCCA GCCCCCGAAA CTCTGGATTC CCGGGCCCTT 
TTCAGTGAGA AAGCGCTGCA GAGCAACCAC TTTGAGCTGA GCTTACGCAC TGAGGCCACG 
CAGGGGCTGG TGCTGTGGAT TGGAAAGGTT GGAGAACGTG CAGACTACAT GGCTCTGGCC 
ATTGTGGATG GGCACCTACA ACTGAGCTAT GACCTAGGCT CCCAGCCAGT TGTGCTGCGC 
TCCACTGTGA AGGTCAACAC CAACCGCTGG CTTCGAGTCA GGGCTCACAG GGAGCACAGG 
GAAGGTTCCC TTCAGGTGGG CAATGAAGCC CCTGTGACTG GCTCTTCCCC GCTGGGTGCC 
ACACAATTGG ACACAGATGG AGCCCTGTGG CTTGGAGGCC TACAGAAGCT TCCTGTGGGG 
CAGGCTCTCC CCAAGGCCTA TGGCACGGGT TTTGTGGGCT GTCTGCGGGA CGTGGTAGTG 
GGCCATCGCC AGCTGCATCT GCTGGAGGAC GCTGTCACCA AACCAGAGCT AAGACCCTGC 
CCCACTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175410.1
CDS	88..5559
Translation	MCMTPTRTPTGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL

Target ORF information:

RefSeq Version	XM_017319921.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus agrin (Agrn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319921.1

ORF Insert Sequence:

ATGTGCATGA CCCCCACCCG CACACCCACA GGGTCCCGGG ACCCCTGTGC CAATGTGACC 
TGCAGTTTCG GTAGTACCTG TGTACCTTCA GCCGATGGAC AGACCGCCTC GTGTCTGTGT 
CCTACAACCT GCTTTGGGGC CCCTGATGGC ACAGTGTGTG GCAGTGATGG TGTTGACTAC 
CCTAGTGAGT GCCAGCTGCT CCGTCATGCC TGTGCCAACC AGGAGCACAT CTTTAAGAAG 
TTCGATGGTC CTTGTGACCC CTGCCAGGGC AGCATGTCAG ACCTGAATCA TATTTGCCGG 
GTGAACCCAC GTACACGGCA CCCAGAAATG CTTCTGCGGC CTGAGAACTG CCCCGCCCAA 
CACACACCTA TCTGTGGAGA TGATGGGGTC ACCTATGAAA ACGACTGTGT CATGAGCCGT 
ATAGGTGCAG CCCGTGGCCT GCTTCTCCAG AAAGTGCGCT CTGGTCAATG CCAGACTCGA 
GACCAGTGCC CGGAGACCTG CCAGTTTAAC TCTGTATGCC TGTCCCGCCG CGGCCGTCCC 
CACTGTTCCT GCGATCGCGT CACCTGTGAT GGGGCTTACA GGCCAGTGTG TGCCCAGGAT 
GGGCACACGT ATGACAATGA CTGTTGGCGC CAACAGGCCG AGTGTCGACA ACAGCAGACC 
ATTCCCCCCA AGCACCAGGG CCCGTGTGAC CAGACCCCAT CCCCGTGCCG TGGAGCGCAG 
TGTGCATTTG GGGCAACATG CACAGTGAAG AATGGGAAAG CTGTGTGCGA GTGCCAGCGG 
GTGTGCTCGG GCGGCTACGA TCCTGTGTGC GGCAGTGATG GTGTCACTTA CGGCAGTGTG 
TGCGAGCTGG AATCCATGGC CTGTACCCTT GGGCGGGAAA TCCGAGTGGC CCGCAGAGGA 
CCGTGTGACC GATGTGGGCA GTGCCGGTTT GGATCCTTGT GCGAGGTGGA GACTGGACGC 
TGTGTGTGCC CCTCTGAGTG TGTGGAGTCA GCCCAGCCCG TATGTGGCTC TGACGGACAC 
ACATATGCTA GTGAATGTGA GCTGCATGTC CACGCCTGTA CACACCAGAT CAGCCTATAC 
GTGGCCTCAG CCGGACACTG CCAGACCTGT GGAGAAACAG TTTGTACCTT TGGGGCTGTG 
TGCTCAGCTG GACAGTGTGT ATGTCCCCGT TGTGAGCACC CCCCACCTGG CCCTGTGTGC 
GGCAGTGATG GCGTCACCTA CCTCAGTGCC TGTGAGCTCC GAGAGGCTGC CTGTCAGCAG 
CAGGTACAAA TTGAGGAGGC CCGTGCAGGG CCGTGTGAGC CGGCTGAGTG TGGCTCAGGG 
GGCTCTGGGT CTGGGGAAGA CAATGCGTGT GAGCAGGAGC TGTGTCGGCA GCATGGTGGT 
GTCTGGGATG AGGACTCAGA AGACGGGCCG TGTGTCTGTG ACTTTAGTTG CCAGAGTGTC 
CTTAAAAGCC CAGTGTGTGG CTCAGATGGA GTCACCTATA GCACGGAGTG CCATCTGAAG 
AAGGCCAGAT GTGAAGCGCG GCAAGAGCTG TACGTCGCTG CTCAGGGAGC CTGCCGGGGC 
CCTACCTTGG CTCCACTGCT ACCTATGGCC TCCCCACACT GTGCCCAAAC CCCCTATGGC 
TGCTGCCAGG ACAATGTCAC TGCTGCCCAG GGTGTGGGCT TGGCTGGCTG TCCCAGCACC 
TGCCATTGCA ACCCACACGG CTCCTATAGC GGCACTTGTG ACCCAGTCAC AGGGCAGTGC 
TCCTGCCGAC CAGGTGTAGG AGGCCTCAGG TGTGATCGCT GTGAGCCTGG CTTCTGGAAC 
TTCCGTGGCA TTGTCACCGA TGGACATAGT GGTTGCACTC CCTGCAGCTG TGACCCTCGG 
GGTGCTGTAA GAGATGACTG TGAGCAGATG ACTGGATTGT GTTCCTGTAG ACCTGGTGTG 
GCTGGTCCCA AGTGTGGGCA GTGTCCAGAT GGTCAAGCCC TGGGCCATCT TGGCTGTGAA 
GCAGATCCCA CAACACCAGT GACTTGTGTG GAAATGCACT GTGAGTTTGG CGCCTCCTGC 
GTAGAGGAGG CTGGTTTTGC CCAGTGTGTC TGCCCAACTC TCACATGTCC AGAGGCTAAC 
TCTACCAAGG TCTGTGGATC AGATGGTGTC ACATACGGCA ATGAATGCCA GCTGAAGACC 
ATTGCCTGCC GCCAGCGTCT GGACATCTCC ATTCAGAGTC TTGGTCCATG CCGGGAGAGT 
GTTGCTCCTG GGGTTTCCCC TACATCTGCA TCTATGACCA CCCCAAGGCA TATCCTGAGC 
AGGACACTGG CGTCTCCCCA CAGCAGCCTT CCTCTGTCTC CCAGCACTAC TGCCCATGAT 
TGGCCCACCC CATTACCCAC ATCACCTCAG ACCGTAGTCG GCACCCCCAG GAGCACTGCA 
GCCACACCCT CTGATGTGGC CAGTCTTGCT ACAGCGATCT TCAGGGAATC TGGCAGCACC 
AACGGCAGTG GCGATGAGGA GCTCAGTGGC GATGAGGAGG CCAGTGGGGG CGGGTCTGGG 
GGACTTGAGC CCCCGGTGGG CAGCGTTGTG GTGACCCACG GGCCACCCAT CGAGAGGGCT 
TCCTGTTACA ACTCACCTTT GGGCTGCTGC TCAGATGGCA AGACACCCTC ACTGGACTCA 
GAAGGCTCCA ACTGTCCAGC TACCAAGGCA TTCCAGGGCG TGCTGGAGCT TGAGGGGGTC 
GAGGGACAGG AACTGTTCTA CACACCAGAG ATGGCTGACC CCAAGTCAGA GTTGTTTGGG 
GAGACTGCAA GGAGCATTGA GAGCACGCTG GACGACCTGT TCCGGAATTC GGATGTTAAG 
AAGGACTTCT GGAGCATCCG CCTACGGGAA CTGGGGCCTG GCAAATTAGT CCGTGCCATT 
GTGGATGTTC ACTTTGACCC CACCACAGCC TTCCAGGCAC CAGATGTGGG TCAGGCCTTG 
CTCCAACAGA TCCAGGTATC CAGGCCGTGG GCCCTGGCAG TGAGGAGGCC TCTGCGGGAG 
CATGTGCGAT TCTTGGACTT TGACTGGTTT CCCACTTTTT TTACGGGAGC TGCAACAGGA 
ACCACAGCTG CTGTGGCCAC AGCCAGAGCC ACCACTGTGA GCCGACTGTC TGCCTCTTCT 
GTCACCCCAC GAGTCTACCC CAGTTACACC AGCCGGCCTG TTGGCAGAAC TACGGCACCG 
CTAACCACTC GCCGGCCACC AACCACTACC GCCAGTATTG ACCGACCTCG GACTCCAGGC 
CCGCAACGGC CCCCAAAGTC CTGTGATTCC CAGCCTTGCC TCCACGGAGG TACCTGCCAG 
GACCTGGATT CTGGCAAGGG TTTCAGCTGC AGCTGTACTG CAGGCAGGGC TGGCACTGTC 
TGTGAGAAAG TGCAGCTCCC CTCTGTGCCA GCTTTTAAGG GCCACTCCTT CTTGGCCTTC 
CCCACCCTCC GAGCCTACCA CACGCTGCGT CTGGCACTAG AATTCCGGGC GCTGGAGACA 
GAGGGACTGC TGCTCTACAA TGGCAATGCA CGTGGCAAAG ATTTCCTGGC TCTGGCTCTG 
TTGGATGGTC ATGTACAGTT CAGGTTCGAC ACGGGCTCAG GGCCGGCGGT GCTAACAAGC 
TTAGTGCCAG TGGAACCGGG ACGGTGGCAC CGCCTCGAGT TGTCACGGCA TTGGCGGCAG 
GGCACACTTT CTGTGGATGG CGAGGCTCCT GTTGTAGGTG AAAGTCCGAG TGGCACTGAT 
GGCCTCAACT TGGACACGAA GCTCTATGTG GGTGGTCTCC CAGAAGAACA AGTTGCCACG 
GTGCTTGATC GGACCTCTGT GGGCATCGGC CTGAAAGGAT GCATTCGTAT GTTGGACATC 
AACAACCAGC AGCTGGAGCT GAGCGATTGG CAGAGGGCTG TGGTTCAAAG CTCTGGTGTG 
GGGGAATGCG GAGACCATCC CTGCTCACCT AACCCCTGCC ATGGCGGGGC CCTCTGCCAG 
GCCCTGGAGG CTGGCGTGTT CCTCTGTCAG TGCCCACCTG GCCGCTTTGG CCCAACTTGT 
GCAGATGAAA AGAACCCCTG CCAACCGAAC CCCTGCCACG GGTCAGCCCC CTGCCATGTG 
CTTTCCAGGG GTGGGGCCAA GTGTGCGTGC CCCCTGGGAC GCAGTGGTTC CTTCTGTGAG 
ACAGTCCTGG AGAATGCTGG CTCCCGGCCC TTCCTGGCTG ACTTTAATGG CTTCTCCTAC 
CTGGAACTGA AAGGCTTGCA CACCTTCGAG AGAGACCTAG GGGAGAAGAT GGCGCTGGAG 
ATGGTGTTCT TGGCTCGTGG GCCCAGTGGC TTACTCCTCT ACAATGGGCA GAAGACGGAT 
GGCAAGGGGG ACTTTGTATC CCTGGCCCTG CATAACCGGC ACCTAGAGTT CCGCTATGAC 
CTTGGCAAGG GGGCTGCAAT CATCAGGAGC AAAGAGCCCA TAGCCCTGGG CACCTGGGTT 
AGGGTATTCC TGGAACGAAA TGGCCGCAAG GGTGCCCTTC AAGTGGGTGA TGGGCCCCGT 
GTGCTAGGGG AATCTCCGAA ATCCCGCAAG GTCCCGCACA CCATGCTCAA CCTCAAGGAG 
CCCCTCTATG TGGGGGGAGC TCCTGACTTC AGCAAGCTGG CTCGGGGCGC TGCAGTGGCC 
TCCGGCTTTG ATGGTGCCAT CCAGCTGGTG TCTCTAAGAG GCCATCAGCT GCTGACTCAG 
GAGCATGTGT TGCGGGCAGT AGATGTAGCG CCTTTTGCAG GCCACCCTTG TACCCAGGCC 
GTGGACAACC CCTGCCTTAA TGGGGGCTCC TGTATCCCGA GGGAAGCCAC TTATGAGTGC 
CTGTGTCCTG GGGGCTTCTC TGGGCTGCAC TGCGAGAAGG GGATAGTTGA GAAGTCAGTG 
GGGGACCTAG AAACACTGGC CTTTGATGGG CGGACCTACA TCGAGTACCT CAATGCTGTG 
ACTGAGAGCG AGCTGACCAA TGAGATCCCA GCCCCCGAAA CTCTGGATTC CCGGGCCCTT 
TTCAGTGAGA AAGCGCTGCA GAGCAACCAC TTTGAGCTGA GCTTACGCAC TGAGGCCACG 
CAGGGGCTGG TGCTGTGGAT TGGAAAGGTT GGAGAACGTG CAGACTACAT GGCTCTGGCC 
ATTGTGGATG GGCACCTACA ACTGAGCTAT GACCTAGGCT CCCAGCCAGT TGTGCTGCGC 
TCCACTGTGA AGGTCAACAC CAACCGCTGG CTTCGAGTCA GGGCTCACAG GGAGCACAGG 
GAAGGTTCCC TTCAGGTGGG CAATGAAGCC CCTGTGACTG GCTCTTCCCC GCTGGGTGCC 
ACACAATTGG ACACAGATGG AGCCCTGTGG CTTGGAGGCC TACAGAAGCT TCCTGTGGGG 
CAGGCTCTCC CCAAGGCCTA TGGCACGGGT TTTGTGGGCT GTCTGCGGGA CGTGGTAGTG 
GGCCATCGCC AGCTGCATCT GCTGGAGGAC GCTGTCACCA AACCAGAGCT AAGACCCTGC 
CCCACTCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Agrn
Entrez Gene ID	11603
Full Name	agrin
Synonyms	Agrin,nmf380
General protein information	Preferred Names agrin Names agrin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E2\|4 88.55 cM

Canis lupus familiaris (dog)	AGRN	XP_005620416.1
Homo sapiens (human)	AGRN	NP_940978.2
Rattus norvegicus (Norway rat)	Agrn	NP_786930.1
Caenorhabditis elegans (roundworm)	agr-1	NP_001022152.3
Xenopus tropicalis (tropical clawed frog)	agrn	XP_002936430.2
	AGRN	XP_001088755.2
Mus musculus (house mouse)	Agrn	NP_067617.3
Gallus gallus (chicken)	AGRN	NP_990858.1
Pan troglodytes (chimpanzee)	LOC100609326	XP_003319002.1
Bos taurus (cattle)	AGRN	XP_005217262.1
Danio rerio (zebrafish)	hm:gc12	NP_001170923.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Agrn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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