ORF » Species Summary » Danio rerio » MID1 cDNA ORF clone

MID1 cDNA ORF clone, Danio rerio(Zebrafish)

The following MID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa49417 XM_009305012.3
Latest version!		 Danio rerio midline 1 (mid1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa15606 XM_009305010.3
Latest version!		 Danio rerio midline 1 (mid1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa15606 XM_009305011.2
Latest version!		 Danio rerio midline 1 (mid1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa15606 XM_009305009.3
Latest version!		 Danio rerio midline 1 (mid1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa15606 XM_002663432.6
Latest version!		 Danio rerio midline 1 (mid1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
ODa15606 XM_009305010.2 Danio rerio midline 1 (mid1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa15606 XM_009305009.2 Danio rerio midline 1 (mid1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa49417 XM_009305012.2 Danio rerio midline 1 (mid1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa15606 XM_002663432.5 Danio rerio midline 1 (mid1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MID1 ( XM_009305012.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303287.1 Danio rerio midline 1 (mid1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa49417
    Clone ID Related Accession (Same CDS sequence) XM_009305012.2 , XM_009305012.3
    Accession Version XM_009305012.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGATGCGG GACGGCTGCA AATGTTGGAT TCCAACCTCA GCAATTTGAT CAAGAGGAAC 
    AGCGAGCTGG AAAACCTGAT GGCAAAGTTG ATCCAGACCT GTCAGCATGT TGAGCACAAT 
    GCGGCTCATC AGGAGAGTAA GCTGACGGAG GAGTGCGACA CACTGATCAA CATCGTCCAG 
    CAGAGGCGAC AGATCATCGG CACCAAGATC AAAGAGGGAA AGGTGGTTCG CTTGAGAAAG 
    CTGGCTCAGC AGATCGCCAA CTGCAAGCAG TTTGTGGAGA GATCGTCCTC GCTCATCATT 
    CAAGCCGATC AAGCTCTGAA GGAGACGGAT CACGCTCGAT TCCTGCAGAT GGCCAAGAGC 
    ATCTCCGAGA GAGTCTCCAT GGCGACTGCG TCCTCACAGG TCTTGATCCC TGAGATAAAC 
    CTGAACGACA CGTTTGACGC GTTCGCATTG GACTTCTCCA GAGAGAAGAA GATGCTGGAA 
    GGTCTGGATT ACCTGACAGC TCCGAATGCT CCCACCATCC GCGAGGAGCT GTGCACCGCG 
    TCTCACGACA CCATCACGGT GCACTGGACA TCAGAGGACG AGTTCAGTGT GGTTTCATAC 
    GAGCTCCAGT ACACCATCTC CACCGGACAG GCCAACGTAG TCAGTCTGTG TAACTCGATG 
    GACAGCTGGA TGATCGTGCC CAACATTAAG CAGAATCACT ACACCGTTCA CGGTCTGCAG 
    AGCGGCACCA AATACATCTT CATCGTGAAG GCCATGAATC AGGCGGGGAG CCGCAGCAGC 
    CCACCGGGAC AACTCAAGAC CAACAGTCAG CCATTTAAAC TGGACCCGAA GTCGGCCCAT 
    AGGAAGTTGA AGGTGTCACA TGACAATCTG ACAGTGGAGC GGGACGAAGC GTCCTCCATG 
    AAGAGCCACG CACAGGACCG ATTTGGAGGC ACCGGAAACT ACGGTGTGAC TGGAAACGTG 
    TACATCGACA GCGGACGCCA CTACTGGGAG GTGCTGATTG GAGGAAGCAC ATGGTTTGCC 
    ATCGGCATCG CCTACAAATC AGCCCCCAAA CACGGCTGGG TGGGAAAGGA CTCGACCTCC 
    TGGGTGTTGA GCCGATGTAA CACCTCGTGG GCGGTTCGTC ACAACAGTAA AGAGTTTCCC 
    ATCGAGCCGT CTCCACACAT GCGGCGTCTG GGCGTCCTGC TGGACTACGA CTCTGGATCT 
    CTGGCGTTTT ACGATGCAGC GGGAACGCAA CACCTCTACA CCTTCGACAT CGCCTTCTCA 
    CAGCCCATCT GCCCTGTTTT TAGCGTGTGG AATAAGTGTT TGACCGTCCT GACGGGGCTG 
    CCCATTCCTG ACCATTTAGA GATCAGTGAC CCGCATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303287.1
    CDS36..1397
    Translation

    Target ORF information:

    RefSeq Version XM_009305012.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009305012.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGATGCGG GACGGCTGCA AATGTTGGAT TCCAACCTCA GCAATTTGAT CAAGAGGAAC 
    AGCGAGCTGG AAAACCTGAT GGCAAAGTTG ATCCAGACCT GTCAGCATGT TGAGCACAAT 
    GCGGCTCATC AGGAGAGTAA GCTGACGGAG GAGTGCGACA CACTGATCAA CATCGTCCAG 
    CAGAGGCGAC AGATCATCGG CACCAAGATC AAAGAGGGAA AGGTGGTTCG CTTGAGAAAG 
    CTGGCTCAGC AGATCGCCAA CTGCAAGCAG TTTGTGGAGA GATCGTCCTC GCTCATCATT 
    CAAGCCGATC AAGCTCTGAA GGAGACGGAT CACGCTCGAT TCCTGCAGAT GGCCAAGAGC 
    ATCTCCGAGA GAGTCTCCAT GGCGACTGCG TCCTCACAGG TCTTGATCCC TGAGATAAAC 
    CTGAACGACA CGTTTGACGC GTTCGCATTG GACTTCTCCA GAGAGAAGAA GATGCTGGAA 
    GGTCTGGATT ACCTGACAGC TCCGAATGCT CCCACCATCC GCGAGGAGCT GTGCACCGCG 
    TCTCACGACA CCATCACGGT GCACTGGACA TCAGAGGACG AGTTCAGTGT GGTTTCATAC 
    GAGCTCCAGT ACACCATCTC CACCGGACAG GCCAACGTAG TCAGTCTGTG TAACTCGATG 
    GACAGCTGGA TGATCGTGCC CAACATTAAG CAGAATCACT ACACCGTTCA CGGTCTGCAG 
    AGCGGCACCA AATACATCTT CATCGTGAAG GCCATGAATC AGGCGGGGAG CCGCAGCAGC 
    CCACCGGGAC AACTCAAGAC CAACAGTCAG CCATTTAAAC TGGACCCGAA GTCGGCCCAT 
    AGGAAGTTGA AGGTGTCACA TGACAATCTG ACAGTGGAGC GGGACGAAGC GTCCTCCATG 
    AAGAGCCACG CACAGGACCG ATTTGGAGGC ACCGGAAACT ACGGTGTGAC TGGAAACGTG 
    TACATCGACA GCGGACGCCA CTACTGGGAG GTGCTGATTG GAGGAAGCAC ATGGTTTGCC 
    ATCGGCATCG CCTACAAATC AGCCCCCAAA CACGGCTGGG TGGGAAAGGA CTCGACCTCC 
    TGGGTGTTGA GCCGATGTAA CACCTCGTGG GCGGTTCGTC ACAACAGTAA AGAGTTTCCC 
    ATCGAGCCGT CTCCACACAT GCGGCGTCTG GGCGTCCTGC TGGACTACGA CTCTGGATCT 
    CTGGCGTTTT ACGATGCAGC GGGAACGCAA CACCTCTACA CCTTCGACAT CGCCTTCTCA 
    CAGCCCATCT GCCCTGTTTT TAGCGTGTGG AATAAGTGTT TGACCGTCCT GACGGGGCTG 
    CCCATTCCTG ACCATTTAGA GATCAGTGAC CCGCATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID1 ( XM_009305012.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303287.1 Danio rerio midline 1 (mid1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa49417
    Clone ID Related Accession (Same CDS sequence) XM_009305012.2 , XM_009305012.3
    Accession Version XM_009305012.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305012.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGATGCGG GACGGCTGCA AATGTTGGAT TCCAACCTCA GCAATTTGAT CAAGAGGAAC 
    AGCGAGCTGG AAAACCTGAT GGCAAAGTTG ATCCAGACCT GTCAGCATGT TGAGCACAAT 
    GCGGCTCATC AGGAGAGTAA GCTGACGGAG GAGTGCGACA CACTGATCAA CATCGTCCAG 
    CAGAGGCGAC AGATCATCGG CACCAAGATC AAAGAGGGAA AGGTGGTTCG CTTGAGAAAG 
    CTGGCTCAGC AGATCGCCAA CTGCAAGCAG TTTGTGGAGA GATCGTCCTC GCTCATCATT 
    CAAGCCGATC AAGCTCTGAA GGAGACGGAT CACGCTCGAT TCCTGCAGAT GGCCAAGAGC 
    ATCTCCGAGA GAGTCTCCAT GGCGACTGCG TCCTCACAGG TCTTGATCCC TGAGATAAAC 
    CTGAACGACA CGTTTGACGC GTTCGCATTG GACTTCTCCA GAGAGAAGAA GATGCTGGAA 
    GGTCTGGATT ACCTGACAGC TCCGAATGCT CCCACCATCC GCGAGGAGCT GTGCACCGCG 
    TCTCACGACA CCATCACGGT GCACTGGACA TCAGAGGACG AGTTCAGTGT GGTTTCATAC 
    GAGCTCCAGT ACACCATCTC CACCGGACAG GCCAACGTAG TCAGTCTGTG TAACTCGATG 
    GACAGCTGGA TGATCGTGCC CAACATTAAG CAGAATCACT ACACCGTTCA CGGTCTGCAG 
    AGCGGCACCA AATACATCTT CATCGTGAAG GCCATGAATC AGGCGGGGAG CCGCAGCAGC 
    CCACCGGGAC AACTCAAGAC CAACAGTCAG CCATTTAAAC TGGACCCGAA GTCGGCCCAT 
    AGGAAGTTGA AGGTGTCACA TGACAATCTG ACAGTGGAGC GGGACGAAGC GTCCTCCATG 
    AAGAGCCACG CACAGGACCG ATTTGGAGGC ACCGGAAACT ACGGTGTGAC TGGAAACGTG 
    TACATCGACA GCGGACGCCA CTACTGGGAG GTGCTGATTG GAGGAAGCAC ATGGTTTGCC 
    ATCGGCATCG CCTACAAATC AGCCCCCAAA CACGGCTGGG TGGGAAAGGA CTCGACCTCC 
    TGGGTGTTGA GCCGATGTAA CACCTCGTGG GCGGTTCGTC ACAACAGTAA AGAGTTTCCC 
    ATCGAGCCGT CTCCACACAT GCGGCGTCTG GGCGTCCTGC TGGACTACGA CTCTGGATCT 
    CTGGCGTTTT ACGATGCAGC GGGAACGCAA CACCTCTACA CCTTCGACAT CGCCTTCTCA 
    CAGCCCATCT GCCCTGTTTT TAGCGTGTGG AATAAGTGTT TGACCGTCCT GACGGGGCTG 
    CCCATTCCTG ACCATTTAGA GATCAGTGAC CCGCATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303287.1
    CDS60..1421
    Translation

    Target ORF information:

    RefSeq Version XM_009305012.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009305012.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGATGCGG GACGGCTGCA AATGTTGGAT TCCAACCTCA GCAATTTGAT CAAGAGGAAC 
    AGCGAGCTGG AAAACCTGAT GGCAAAGTTG ATCCAGACCT GTCAGCATGT TGAGCACAAT 
    GCGGCTCATC AGGAGAGTAA GCTGACGGAG GAGTGCGACA CACTGATCAA CATCGTCCAG 
    CAGAGGCGAC AGATCATCGG CACCAAGATC AAAGAGGGAA AGGTGGTTCG CTTGAGAAAG 
    CTGGCTCAGC AGATCGCCAA CTGCAAGCAG TTTGTGGAGA GATCGTCCTC GCTCATCATT 
    CAAGCCGATC AAGCTCTGAA GGAGACGGAT CACGCTCGAT TCCTGCAGAT GGCCAAGAGC 
    ATCTCCGAGA GAGTCTCCAT GGCGACTGCG TCCTCACAGG TCTTGATCCC TGAGATAAAC 
    CTGAACGACA CGTTTGACGC GTTCGCATTG GACTTCTCCA GAGAGAAGAA GATGCTGGAA 
    GGTCTGGATT ACCTGACAGC TCCGAATGCT CCCACCATCC GCGAGGAGCT GTGCACCGCG 
    TCTCACGACA CCATCACGGT GCACTGGACA TCAGAGGACG AGTTCAGTGT GGTTTCATAC 
    GAGCTCCAGT ACACCATCTC CACCGGACAG GCCAACGTAG TCAGTCTGTG TAACTCGATG 
    GACAGCTGGA TGATCGTGCC CAACATTAAG CAGAATCACT ACACCGTTCA CGGTCTGCAG 
    AGCGGCACCA AATACATCTT CATCGTGAAG GCCATGAATC AGGCGGGGAG CCGCAGCAGC 
    CCACCGGGAC AACTCAAGAC CAACAGTCAG CCATTTAAAC TGGACCCGAA GTCGGCCCAT 
    AGGAAGTTGA AGGTGTCACA TGACAATCTG ACAGTGGAGC GGGACGAAGC GTCCTCCATG 
    AAGAGCCACG CACAGGACCG ATTTGGAGGC ACCGGAAACT ACGGTGTGAC TGGAAACGTG 
    TACATCGACA GCGGACGCCA CTACTGGGAG GTGCTGATTG GAGGAAGCAC ATGGTTTGCC 
    ATCGGCATCG CCTACAAATC AGCCCCCAAA CACGGCTGGG TGGGAAAGGA CTCGACCTCC 
    TGGGTGTTGA GCCGATGTAA CACCTCGTGG GCGGTTCGTC ACAACAGTAA AGAGTTTCCC 
    ATCGAGCCGT CTCCACACAT GCGGCGTCTG GGCGTCCTGC TGGACTACGA CTCTGGATCT 
    CTGGCGTTTT ACGATGCAGC GGGAACGCAA CACCTCTACA CCTTCGACAT CGCCTTCTCA 
    CAGCCCATCT GCCCTGTTTT TAGCGTGTGG AATAAGTGTT TGACCGTCCT GACGGGGCTG 
    CCCATTCCTG ACCATTTAGA GATCAGTGAC CCGCATGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID1 ( XM_009305010.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303285.1 Danio rerio midline 1 (mid1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa15606
    Clone ID Related Accession (Same CDS sequence) XM_009305010.2 , XM_009305011.2 , XM_009305010.3 , XM_009305009.3 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
    Accession Version XM_009305010.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303285.1
    CDS284..2287
    Translation

    Target ORF information:

    RefSeq Version XM_009305010.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009305010.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID1 ( XM_009305011.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303286.1 Danio rerio midline 1 (mid1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa15606
    Clone ID Related Accession (Same CDS sequence) XM_009305010.2 , XM_009305011.2 , XM_009305010.3 , XM_009305009.3 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
    Accession Version XM_009305011.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303286.1
    CDS342..2345
    Translation

    Target ORF information:

    RefSeq Version XM_009305011.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009305011.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID1 ( XM_009305010.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303285.1 Danio rerio midline 1 (mid1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa15606
    Clone ID Related Accession (Same CDS sequence) XM_009305010.2 , XM_009305011.2 , XM_009305010.3 , XM_009305009.3 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
    Accession Version XM_009305010.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303285.1
    CDS403..2406
    Translation

    Target ORF information:

    RefSeq Version XM_009305010.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009305010.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID1 ( XM_009305009.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303284.1 Danio rerio midline 1 (mid1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa15606
    Clone ID Related Accession (Same CDS sequence) XM_009305010.2 , XM_009305011.2 , XM_009305010.3 , XM_009305009.3 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
    Accession Version XM_009305009.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303284.1
    CDS147..2150
    Translation

    Target ORF information:

    RefSeq Version XM_009305009.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009305009.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID1 ( XM_002663432.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002663478.1 Danio rerio midline 1 (mid1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa15606
    Clone ID Related Accession (Same CDS sequence) XM_009305010.2 , XM_009305011.2 , XM_009305010.3 , XM_009305009.3 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
    Accession Version XM_002663432.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002663432.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002663478.1
    CDS188..2191
    Translation

    Target ORF information:

    RefSeq Version XM_002663432.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_002663432.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID1 ( XM_009305009.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303284.1 Danio rerio midline 1 (mid1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa15606
    Clone ID Related Accession (Same CDS sequence) XM_009305010.2 , XM_009305011.2 , XM_009305010.3 , XM_009305009.3 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
    Accession Version XM_009305009.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303284.1
    CDS146..2149
    Translation

    Target ORF information:

    RefSeq Version XM_009305009.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009305009.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MID1 ( XM_002663432.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002663478.1 Danio rerio midline 1 (mid1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa15606
    Clone ID Related Accession (Same CDS sequence) XM_009305010.2 , XM_009305011.2 , XM_009305010.3 , XM_009305009.3 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
    Accession Version XM_002663432.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002663432.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002663478.1
    CDS143..2146
    Translation

    Target ORF information:

    RefSeq Version XM_002663432.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio midline 1 (mid1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_002663432.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
    CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
    CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
    CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
    CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
    ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
    TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
    TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
    CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
    GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
    GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
    CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
    ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
    AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
    GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
    AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
    AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
    ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
    GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
    GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
    GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
    TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
    CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
    TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
    ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
    CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
    CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
    CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
    TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
    AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
    ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
    GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
    TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
    GAGATCAGTG ACCCGCATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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