MID1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID1
Entrez Gene ID	373920
Full Name	midline 1
General protein information	Preferred Names midline 1 Names E3 ubiquitin-protein ligase Midline-1 midline 1 (Opitz/BBB syndrome)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025148585.1	XP_025004353.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_025148536.1	XP_025004304.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_025148597.1	XP_025004365.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_025148518.1	XP_025004286.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_025148626.1	XP_025004394.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
NM_204129.1	NP_989460.1	E3 ubiquitin-protein ligase Midline-1
XM_025148644.1	XP_025004412.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
XM_025148549.1	XP_025004317.1	E3 ubiquitin-protein ligase Midline-1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mid1	NP_034927.2
Homo sapiens (human)	MID1	NP_150632.1
Rattus norvegicus (Norway rat)	Mid1	NP_075216.1
Danio rerio (zebrafish)	mid1	XP_002663478.1
Xenopus tropicalis (tropical clawed frog)	mid1	NP_001006876.1
Bos taurus (cattle)	MID1	NP_001179751.1
Gallus gallus (chicken)	MID1	NP_989460.1
	MID1	XP_001093390.1
Canis lupus familiaris (dog)	MID1	XP_548857.2

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of PP2A activity by Mid1 controls cranial neural crest speed and gangliogenesis.
Latta EJ, Golding JP
Mechanisms of development128(11-12)560-76(2012 Jan-Feb)

Evidence of functional redundancy between MID proteins: implications for the presentation of Opitz syndrome.
Granata A, Savery D, Hazan J, Cheung BM, Lumsden A, Quaderi NA
Developmental biology277(2)417-24(2005 Jan)

The Opitz syndrome gene MID1 is essential for establishing asymmetric gene expression in Hensen's node.
Granata A, Quaderi NA
Developmental biology258(2)397-405(2003 Jun)

Isolation and characterisation of the chick orthologue of the Opitz syndrome gene, Mid1, supports a conserved role in vertebrate development.
Richman JM, Fu KK, Cox LL, Sibbons JP, Cox TC
The International journal of developmental biology46(4)441-8(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suppression of Mid1 function in r2 elevates PP2Ac protein, whilst ectopic expression of Mid1 depletes PP2Ac protein.
Title: Regulation of PP2A activity by Mid1 controls cranial neural crest speed and gangliogenesis.

A mutually repressive relationship between Mid1 and Shh in Hensen's node plays a key role in establishing the avian left-right axis.
Title: Evidence of functional redundancy between MID proteins: implications for the presentation of Opitz syndrome.

The following MID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa24126	NM_204129.1 Latest version!	Gallus gallus midline 1 (MID1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OGa142017	XM_025148549.1 Latest version!	Gallus gallus midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa142017	XM_025148585.1 Latest version!	Gallus gallus midline 1 (MID1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa142017	XM_025148536.1 Latest version!	Gallus gallus midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa142017	XM_025148518.1 Latest version!	Gallus gallus midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa142017	XM_025148597.1 Latest version!	Gallus gallus midline 1 (MID1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa142018	XM_025148644.1 Latest version!	Gallus gallus midline 1 (MID1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa142018	XM_025148626.1 Latest version!	Gallus gallus midline 1 (MID1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID1 ( NM_204129.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989460.1 Gallus gallus midline 1 (MID1), mRNA.

Price & Availability↑

CloneID	OGa24126
Clone ID Related Accession (Same CDS sequence)	NM_204129.1
Accession Version	NM_204129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF374463.1. ##Evidence-Data-START## Transcript exon combination :: AF374463.1, AF269102.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
CAAAATTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ATACTGAACT GGAAACTCTT 
CTGGCAAAAC TCATTCAGAC CTGTCAACAT GTAGAAGTAA ATGCATCTCG CCAAGAAACC 
AAGCTGATGG AAGAATGTGA CCAGCTCATT GAAATAATAC AACAGAGACG ACAAATAATT 
GGAACCAAAA TCAAGGAAGG AAAGGTGGTG AGGTTGAGAA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGCTCGACA TCCCTCATCT CTCAGGCTGA GCAGTCGCTG 
AAGGAGAATG ATCATGCTCG CTTCCTGCAA ACTGCTAAAA ACATCACCGA AAGGGTTTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATTA ATCTCAACGA TACTTTTGAT 
ACTTTCGCAC TTGATTTTAC CAGGGAGAAG AAATTGTTGG AATGCCTTGA TTATCTTACA 
GCTCCCAACC CTCCCACCAT TCGAGAAGAG CTCTGTACAG CTTCTTATGA TACTATTACT 
GTCCACTGGA CATCAGACGA TGAGTTCAGC GTGGTCTCTT ACGAGCTGCA GTACACCATC 
TTCACTGGAC AAGCTAACGT TGTTAGTTTA TGTAACTCAG CCGACAGCTG GATGATTGTT 
CCCAATATCA AACAAAACCA CTACACCGTG CATGGGTTAC AGAGTGGCAC AAAGTACATC 
TTCATTGTTA AGGCTATTAA TCAGGCTGGC AGCAGAAGCA GCGAGCCAGG CAAGCTCAAG 
ACAAACAGTC AGCCATTTAA ACTGGACCCC AAATCTGCTC ATAGAAAGCT GAAAGTGTCT 
CATGACAACT TGACAGTGGA ACGTGATGAA ACCTCCTCCA AAAAGAGTCA TACACCAGAG 
CGATTCACGA GCCAAGGGAG CTACGGAGTA GCTGGCAATG TATTCATTGA CAGTGGACGG 
CATTACTGGG AAGTGGTTAT AAGTGGGAGT ACATGGTATG CCATTGGTAT TTCATACAAG 
TCAGCACCGA AGCATGAGTG GATCGGGAAG AACTCAGCCT CCTGGGTGCT GTGCCGCTGC 
AACAACACAT GGGTGGTGAG ACACAACAGC AAAGAAATCC CAATAGAGCC TGCGCCTCAT 
CTCCGCCGTG TCGGCATTTT GCTGGACTAC GACAATGGTT CCCTTGCTTT TTATGATGCC 
TTGAACTCCC TGCACCTTTA CACCTTTGAC ATTACGTTTG GGCAGCCCGT CTGCCCCACA 
TTCACTGTGT GGAATAAGTG TTTGACCATT ATAACAGGCT TGCCCATCCC TGATCACTTA 
GATTCCTCTG AGCAGCTGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989460.1
CDS	58..2061
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCASNEPVESITAFQCPTCRYVITLSQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDSNSMSSCEKVLCQFCDQDPAQEAVKTCVTCEVSYCEECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHREHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQETKLMEECDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQCIERSTSLISQAEQSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFTREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGISYKSAPKHEWIGKNSASWVLCRCNNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSLAFYDALNSLHLYTFDITFGQPVCPTFTVWNKCLTIITGLPIPDHLDSSEQLA

Target ORF information:

RefSeq Version	NM_204129.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus midline 1 (MID1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204129.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
CAAAATTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ATACTGAACT GGAAACTCTT 
CTGGCAAAAC TCATTCAGAC CTGTCAACAT GTAGAAGTAA ATGCATCTCG CCAAGAAACC 
AAGCTGATGG AAGAATGTGA CCAGCTCATT GAAATAATAC AACAGAGACG ACAAATAATT 
GGAACCAAAA TCAAGGAAGG AAAGGTGGTG AGGTTGAGAA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGCTCGACA TCCCTCATCT CTCAGGCTGA GCAGTCGCTG 
AAGGAGAATG ATCATGCTCG CTTCCTGCAA ACTGCTAAAA ACATCACCGA AAGGGTTTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATTA ATCTCAACGA TACTTTTGAT 
ACTTTCGCAC TTGATTTTAC CAGGGAGAAG AAATTGTTGG AATGCCTTGA TTATCTTACA 
GCTCCCAACC CTCCCACCAT TCGAGAAGAG CTCTGTACAG CTTCTTATGA TACTATTACT 
GTCCACTGGA CATCAGACGA TGAGTTCAGC GTGGTCTCTT ACGAGCTGCA GTACACCATC 
TTCACTGGAC AAGCTAACGT TGTTAGTTTA TGTAACTCAG CCGACAGCTG GATGATTGTT 
CCCAATATCA AACAAAACCA CTACACCGTG CATGGGTTAC AGAGTGGCAC AAAGTACATC 
TTCATTGTTA AGGCTATTAA TCAGGCTGGC AGCAGAAGCA GCGAGCCAGG CAAGCTCAAG 
ACAAACAGTC AGCCATTTAA ACTGGACCCC AAATCTGCTC ATAGAAAGCT GAAAGTGTCT 
CATGACAACT TGACAGTGGA ACGTGATGAA ACCTCCTCCA AAAAGAGTCA TACACCAGAG 
CGATTCACGA GCCAAGGGAG CTACGGAGTA GCTGGCAATG TATTCATTGA CAGTGGACGG 
CATTACTGGG AAGTGGTTAT AAGTGGGAGT ACATGGTATG CCATTGGTAT TTCATACAAG 
TCAGCACCGA AGCATGAGTG GATCGGGAAG AACTCAGCCT CCTGGGTGCT GTGCCGCTGC 
AACAACACAT GGGTGGTGAG ACACAACAGC AAAGAAATCC CAATAGAGCC TGCGCCTCAT 
CTCCGCCGTG TCGGCATTTT GCTGGACTAC GACAATGGTT CCCTTGCTTT TTATGATGCC 
TTGAACTCCC TGCACCTTTA CACCTTTGAC ATTACGTTTG GGCAGCCCGT CTGCCCCACA 
TTCACTGTGT GGAATAAGTG TTTGACCATT ATAACAGGCT TGCCCATCCC TGATCACTTA 
GATTCCTCTG AGCAGCTGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_025148585.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004353.1 Gallus gallus midline 1 (MID1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa142017
Clone ID Related Accession (Same CDS sequence)	XM_025148585.1 , XM_025148536.1 , XM_025148597.1 , XM_025148518.1 , XM_025148549.1
Accession Version	XM_025148585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004353.1
CDS	170..2257
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCASNEPVESITAFQCPTCRYVITLSQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDSNSMSSCEKVLCQFCDQDPAQEAVKTCVTCEVSYCEECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHREHQVAALSERYDKLKELTAFWSPDLAESSGAVTVSLCSAVVGQQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQETKLMEECDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQCIERSTSLISQAEQSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFTREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGISYKSAPKHEWIGKNSASWVLCRCNNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSLAFYDALNSLHLYTFDITFGQPVCPTFTVWNKCLTIITGLPIPDHLDSSEQLA

Target ORF information:

RefSeq Version	XM_025148585.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus midline 1 (MID1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148585.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_025148536.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004304.1 Gallus gallus midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa142017
Clone ID Related Accession (Same CDS sequence)	XM_025148585.1 , XM_025148536.1 , XM_025148597.1 , XM_025148518.1 , XM_025148549.1
Accession Version	XM_025148536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004304.1
CDS	274..2361
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCASNEPVESITAFQCPTCRYVITLSQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDSNSMSSCEKVLCQFCDQDPAQEAVKTCVTCEVSYCEECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHREHQVAALSERYDKLKELTAFWSPDLAESSGAVTVSLCSAVVGQQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQETKLMEECDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQCIERSTSLISQAEQSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFTREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGISYKSAPKHEWIGKNSASWVLCRCNNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSLAFYDALNSLHLYTFDITFGQPVCPTFTVWNKCLTIITGLPIPDHLDSSEQLA

Target ORF information:

RefSeq Version	XM_025148536.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148536.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_025148597.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004365.1 Gallus gallus midline 1 (MID1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa142017
Clone ID Related Accession (Same CDS sequence)	XM_025148585.1 , XM_025148536.1 , XM_025148597.1 , XM_025148518.1 , XM_025148549.1
Accession Version	XM_025148597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004365.1
CDS	1412..3499
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCASNEPVESITAFQCPTCRYVITLSQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDSNSMSSCEKVLCQFCDQDPAQEAVKTCVTCEVSYCEECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHREHQVAALSERYDKLKELTAFWSPDLAESSGAVTVSLCSAVVGQQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQETKLMEECDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQCIERSTSLISQAEQSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFTREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGISYKSAPKHEWIGKNSASWVLCRCNNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSLAFYDALNSLHLYTFDITFGQPVCPTFTVWNKCLTIITGLPIPDHLDSSEQLA

Target ORF information:

RefSeq Version	XM_025148597.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus midline 1 (MID1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148597.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_025148518.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004286.1 Gallus gallus midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa142017
Clone ID Related Accession (Same CDS sequence)	XM_025148585.1 , XM_025148536.1 , XM_025148597.1 , XM_025148518.1 , XM_025148549.1
Accession Version	XM_025148518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004286.1
CDS	193..2280
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCASNEPVESITAFQCPTCRYVITLSQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDSNSMSSCEKVLCQFCDQDPAQEAVKTCVTCEVSYCEECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHREHQVAALSERYDKLKELTAFWSPDLAESSGAVTVSLCSAVVGQQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQETKLMEECDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQCIERSTSLISQAEQSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFTREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGISYKSAPKHEWIGKNSASWVLCRCNNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSLAFYDALNSLHLYTFDITFGQPVCPTFTVWNKCLTIITGLPIPDHLDSSEQLA

Target ORF information:

RefSeq Version	XM_025148518.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148518.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_025148549.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004317.1 Gallus gallus midline 1 (MID1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa142017
Clone ID Related Accession (Same CDS sequence)	XM_025148585.1 , XM_025148536.1 , XM_025148597.1 , XM_025148518.1 , XM_025148549.1
Accession Version	XM_025148549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004317.1
CDS	209..2296
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCASNEPVESITAFQCPTCRYVITLSQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDSNSMSSCEKVLCQFCDQDPAQEAVKTCVTCEVSYCEECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHREHQVAALSERYDKLKELTAFWSPDLAESSGAVTVSLCSAVVGQQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQETKLMEECDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQCIERSTSLISQAEQSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFTREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGISYKSAPKHEWIGKNSASWVLCRCNNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSLAFYDALNSLHLYTFDITFGQPVCPTFTVWNKCLTIITGLPIPDHLDSSEQLA

Target ORF information:

RefSeq Version	XM_025148549.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148549.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
GAACTAACCG CATTCTGGAG TCCAGACCTT GCTGAGAGTT CAGGAGCTGT GACAGTATCA 
CTCTGTAGTG CAGTGGTAGG CCAGCAAAAT TTGGAGAGTA ACCTCACCAA CCTTATTAAG 
AGGAATACTG AACTGGAAAC TCTTCTGGCA AAACTCATTC AGACCTGTCA ACATGTAGAA 
GTAAATGCAT CTCGCCAAGA AACCAAGCTG ATGGAAGAAT GTGACCAGCT CATTGAAATA 
ATACAACAGA GACGACAAAT AATTGGAACC AAAATCAAGG AAGGAAAGGT GGTGAGGTTG 
AGAAAACTGG CTCAGCAGAT TGCAAACTGC AAACAGTGCA TCGAGCGCTC GACATCCCTC 
ATCTCTCAGG CTGAGCAGTC ACTGAAGGAG AACGATCACG CTCGCTTCCT GCAAACTGCT 
AAAAACATCA CTGAAAGGGT TTCCATGGCA ACTGCATCCT CCCAGGTTCT AATTCCTGAA 
ATTAATCTCA ACGATACTTT TGATACTTTC GCACTTGATT TTACCAGGGA GAAGAAATTG 
TTGGAATGCC TTGATTATCT TACAGCTCCC AACCCTCCCA CCATTCGAGA AGAGCTCTGT 
ACAGCTTCTT ATGATACTAT TACTGTCCAC TGGACATCAG ACGATGAGTT CAGCGTGGTC 
TCTTACGAGC TGCAGTACAC CATCTTCACT GGACAAGCTA ACGTTGTTAG TTTATGTAAC 
TCAGCCGACA GCTGGATGAT TGTTCCCAAT ATCAAACAAA ACCACTACAC CGTGCATGGG 
TTACAGAGTG GCACAAAGTA CATCTTCATT GTTAAGGCTA TTAATCAGGC TGGCAGCAGA 
AGCAGCGAGC CAGGCAAGCT CAAGACAAAC AGTCAGCCAT TTAAACTGGA CCCCAAATCT 
GCTCATAGAA AGCTGAAAGT GTCTCATGAC AATTTGACAG TGGAACGTGA TGAAACCTCC 
TCCAAAAAGA GTCATACACC AGAGCGATTC ACGAGCCAAG GGAGCTATGG AGTAGCTGGC 
AATGTGTTCA TTGACAGCGG ACGGCATTAC TGGGAAGTGG TTATAAGTGG GAGTACATGG 
TATGCCATTG GTATTTCATA CAAGTCAGCA CCGAAGCATG AGTGGATCGG GAAGAACTCA 
GCCTCCTGGG TGCTGTGCCG CTGCAACAAC ACATGGGTGG TGAGACACAA CAGCAAAGAA 
ATCCCAATAG AGCCTGCGCC TCATCTCCGC CGTGTCGGCA TTTTGCTGGA CTACGACAAT 
GGTTCCCTTG CTTTTTATGA TGCCTTGAAC TCCCTGCACC TTTACACCTT TGACATTACG 
TTTGGGCAGC CCGTCTGCCC CACATTCACT GTGTGGAATA AGTGTTTGAC CATTATAACA 
GGCTTGCCCA TCCCTGATCA CTTAGACTCC TCTGAGCAGC TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_025148626.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004394.1 Gallus gallus midline 1 (MID1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa142018
Clone ID Related Accession (Same CDS sequence)	XM_025148626.1 , XM_025148644.1
Accession Version	XM_025148626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
CAAAATTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ATACTGAACT GGAAACTCTT 
CTGGCAAAAC TCATTCAGAC CTGTCAACAT GTAGAAGTAA ATGCATCTCG CCAAGAAACC 
AAGCTGATGG AAGAATGTGA CCAGCTCATT GAAATAATAC AACAGAGACG ACAAATAATT 
GGAACCAAAA TCAAGGAAGG AAAGGTGGTG AGGTTGAGAA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGCTCGACA TCCCTCATCT CTCAGGCTGA GCAGTCACTG 
AAGGAGAACG ATCACGCTCG CTTCCTGCAA ACTGCTAAAA ACATCACTGA AAGGGTTTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATTA ATCTCAACGA TACTTTTGAT 
ACTTTCGCAC TTGATTTTAC CAGGGAGAAG AAATTGTTGG AATGCCTTGA TTATCTTACA 
GCTCCCAACC CTCCCACCAT TCGAGAAGAG CTCTGTACAG CTTCTTATGA TACTATTACT 
GTCCACTGGA CATCAGACGA TGAGTTCAGC GTGGTCTCTT ACGAGCTGCA GTACACCATC 
TTCACTGGAC AAGCTAACGT TGTTAGTTTA TGTAACTCAG CCGACAGCTG GATGATTGTT 
CCCAATATCA AACAAAACCA CTACACCGTG CATGGGTTAC AGAGTGGCAC AAAGTACATC 
TTCATTGTTA AGGCTATTAA TCAGGCTGGC AGCAGAAGCA GCGAGCCAGG CAAGCTCAAG 
ACAAACAGTC AGCCATTTAA ACTGGACCCC AAATCTGCTC ATAGAAAGCT GAAAGTGTCT 
CATGACAATT TGACAGTGGA ACGTGATGAA ACCTCCTCCA AAAAGAGTCA TACACCAGAG 
CGATTCACGA GCCAAGGGAG CTATGGAGTA GCTGGCAATG TGTTCATTGA CAGCGGACGG 
CATTACTGGG AAGTGGTTAT AAGTGGGAGT ACATGGTATG CCATTGGTAT TTCATACAAG 
TCAGCACCGA AGCATGAGTG GATCGGGAAG AACTCAGCCT CCTGGGTGCT GTGCCGCTGC 
AACAACACAT GGGTGGTGAG ACACAACAGC AAAGAAATCC CAATAGAGCC TGCGCCTCAT 
CTCCGCCGTG TCGGCATTTT GCTGGACTAC GACAATGGTT CCCTTGCTTT TTATGATGCC 
TTGAACTCCC TGCACCTTTA CACCTTTGAC ATTACGTTTG GGCAGCCCGT CTGCCCCACA 
TTCACTGTGT GGAATAAGTG TTTGACCATT ATAACAGGCT TGCCCATCCC TGATCACTTA 
GACTCCTCTG AGCAGCTGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004394.1
CDS	361..2364
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCASNEPVESITAFQCPTCRYVITLSQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDSNSMSSCEKVLCQFCDQDPAQEAVKTCVTCEVSYCEECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHREHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQETKLMEECDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQCIERSTSLISQAEQSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFTREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGISYKSAPKHEWIGKNSASWVLCRCNNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSLAFYDALNSLHLYTFDITFGQPVCPTFTVWNKCLTIITGLPIPDHLDSSEQLA

Target ORF information:

RefSeq Version	XM_025148626.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus midline 1 (MID1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148626.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
CAAAATTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ATACTGAACT GGAAACTCTT 
CTGGCAAAAC TCATTCAGAC CTGTCAACAT GTAGAAGTAA ATGCATCTCG CCAAGAAACC 
AAGCTGATGG AAGAATGTGA CCAGCTCATT GAAATAATAC AACAGAGACG ACAAATAATT 
GGAACCAAAA TCAAGGAAGG AAAGGTGGTG AGGTTGAGAA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGCTCGACA TCCCTCATCT CTCAGGCTGA GCAGTCACTG 
AAGGAGAACG ATCACGCTCG CTTCCTGCAA ACTGCTAAAA ACATCACTGA AAGGGTTTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATTA ATCTCAACGA TACTTTTGAT 
ACTTTCGCAC TTGATTTTAC CAGGGAGAAG AAATTGTTGG AATGCCTTGA TTATCTTACA 
GCTCCCAACC CTCCCACCAT TCGAGAAGAG CTCTGTACAG CTTCTTATGA TACTATTACT 
GTCCACTGGA CATCAGACGA TGAGTTCAGC GTGGTCTCTT ACGAGCTGCA GTACACCATC 
TTCACTGGAC AAGCTAACGT TGTTAGTTTA TGTAACTCAG CCGACAGCTG GATGATTGTT 
CCCAATATCA AACAAAACCA CTACACCGTG CATGGGTTAC AGAGTGGCAC AAAGTACATC 
TTCATTGTTA AGGCTATTAA TCAGGCTGGC AGCAGAAGCA GCGAGCCAGG CAAGCTCAAG 
ACAAACAGTC AGCCATTTAA ACTGGACCCC AAATCTGCTC ATAGAAAGCT GAAAGTGTCT 
CATGACAATT TGACAGTGGA ACGTGATGAA ACCTCCTCCA AAAAGAGTCA TACACCAGAG 
CGATTCACGA GCCAAGGGAG CTATGGAGTA GCTGGCAATG TGTTCATTGA CAGCGGACGG 
CATTACTGGG AAGTGGTTAT AAGTGGGAGT ACATGGTATG CCATTGGTAT TTCATACAAG 
TCAGCACCGA AGCATGAGTG GATCGGGAAG AACTCAGCCT CCTGGGTGCT GTGCCGCTGC 
AACAACACAT GGGTGGTGAG ACACAACAGC AAAGAAATCC CAATAGAGCC TGCGCCTCAT 
CTCCGCCGTG TCGGCATTTT GCTGGACTAC GACAATGGTT CCCTTGCTTT TTATGATGCC 
TTGAACTCCC TGCACCTTTA CACCTTTGAC ATTACGTTTG GGCAGCCCGT CTGCCCCACA 
TTCACTGTGT GGAATAAGTG TTTGACCATT ATAACAGGCT TGCCCATCCC TGATCACTTA 
GACTCCTCTG AGCAGCTGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_025148644.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004412.1 Gallus gallus midline 1 (MID1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa142018
Clone ID Related Accession (Same CDS sequence)	XM_025148626.1 , XM_025148644.1
Accession Version	XM_025148644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
CAAAATTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ATACTGAACT GGAAACTCTT 
CTGGCAAAAC TCATTCAGAC CTGTCAACAT GTAGAAGTAA ATGCATCTCG CCAAGAAACC 
AAGCTGATGG AAGAATGTGA CCAGCTCATT GAAATAATAC AACAGAGACG ACAAATAATT 
GGAACCAAAA TCAAGGAAGG AAAGGTGGTG AGGTTGAGAA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGCTCGACA TCCCTCATCT CTCAGGCTGA GCAGTCACTG 
AAGGAGAACG ATCACGCTCG CTTCCTGCAA ACTGCTAAAA ACATCACTGA AAGGGTTTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATTA ATCTCAACGA TACTTTTGAT 
ACTTTCGCAC TTGATTTTAC CAGGGAGAAG AAATTGTTGG AATGCCTTGA TTATCTTACA 
GCTCCCAACC CTCCCACCAT TCGAGAAGAG CTCTGTACAG CTTCTTATGA TACTATTACT 
GTCCACTGGA CATCAGACGA TGAGTTCAGC GTGGTCTCTT ACGAGCTGCA GTACACCATC 
TTCACTGGAC AAGCTAACGT TGTTAGTTTA TGTAACTCAG CCGACAGCTG GATGATTGTT 
CCCAATATCA AACAAAACCA CTACACCGTG CATGGGTTAC AGAGTGGCAC AAAGTACATC 
TTCATTGTTA AGGCTATTAA TCAGGCTGGC AGCAGAAGCA GCGAGCCAGG CAAGCTCAAG 
ACAAACAGTC AGCCATTTAA ACTGGACCCC AAATCTGCTC ATAGAAAGCT GAAAGTGTCT 
CATGACAATT TGACAGTGGA ACGTGATGAA ACCTCCTCCA AAAAGAGTCA TACACCAGAG 
CGATTCACGA GCCAAGGGAG CTATGGAGTA GCTGGCAATG TGTTCATTGA CAGCGGACGG 
CATTACTGGG AAGTGGTTAT AAGTGGGAGT ACATGGTATG CCATTGGTAT TTCATACAAG 
TCAGCACCGA AGCATGAGTG GATCGGGAAG AACTCAGCCT CCTGGGTGCT GTGCCGCTGC 
AACAACACAT GGGTGGTGAG ACACAACAGC AAAGAAATCC CAATAGAGCC TGCGCCTCAT 
CTCCGCCGTG TCGGCATTTT GCTGGACTAC GACAATGGTT CCCTTGCTTT TTATGATGCC 
TTGAACTCCC TGCACCTTTA CACCTTTGAC ATTACGTTTG GGCAGCCCGT CTGCCCCACA 
TTCACTGTGT GGAATAAGTG TTTGACCATT ATAACAGGCT TGCCCATCCC TGATCACTTA 
GACTCCTCTG AGCAGCTGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004412.1
CDS	272..2275
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCASNEPVESITAFQCPTCRYVITLSQRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDSNSMSSCEKVLCQFCDQDPAQEAVKTCVTCEVSYCEECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHREHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQETKLMEECDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQCIERSTSLISQAEQSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFTREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGISYKSAPKHEWIGKNSASWVLCRCNNTWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSLAFYDALNSLHLYTFDITFGQPVCPTFTVWNKCLTIITGLPIPDHLDSSEQLA

Target ORF information:

RefSeq Version	XM_025148644.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus midline 1 (MID1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148644.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA ACTGACCTGC CCTATCTGTC TCGAGCTGTT TGAGGACCCG 
CTGCTGCTGC CCTGCGCTCA CAGTCTCTGC TTCAACTGCG CGCACCGCAT CCTGGTCTCC 
CACTGCGCCT CCAACGAGCC GGTGGAGTCT ATCACCGCCT TCCAGTGCCC CACCTGCCGC 
TATGTCATCA CCCTCAGCCA GCGCGGTCTA GAGGGGCTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACCGCTTTCA GAAAGCCTCG GTGAGCGGGC CCAACTCCCC CAGCGAGACC 
CGTCGGGAGC GGGCATTCGA CAGCAACAGC ATGTCCTCCT GCGAGAAGGT CCTCTGCCAG 
TTCTGCGACC AGGACCCTGC CCAGGAGGCA GTGAAGACGT GCGTGACCTG CGAGGTCTCC 
TACTGCGAGG AGTGCCTGAA AGCCACGCAC CCCAACAAGA AGCCTTTCAC CGGCCATCGG 
CTGATCGAGC CCATCCCGGA CTCTCACATC AGGGGATTAA TGTGCTTGGA GCACGAGGAC 
GAGAAGGTGA ACATGTACTG CGTGACAGAT GACCAGCTGA TTTGCGCCCT GTGCAAGCTG 
GTCGGGCGGC ACCGGGAACA TCAAGTGGCA GCTCTAAGCG AGCGCTACGA CAAGCTGAAG 
CAAAATTTGG AGAGTAACCT CACCAACCTT ATTAAGAGGA ATACTGAACT GGAAACTCTT 
CTGGCAAAAC TCATTCAGAC CTGTCAACAT GTAGAAGTAA ATGCATCTCG CCAAGAAACC 
AAGCTGATGG AAGAATGTGA CCAGCTCATT GAAATAATAC AACAGAGACG ACAAATAATT 
GGAACCAAAA TCAAGGAAGG AAAGGTGGTG AGGTTGAGAA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATCGA GCGCTCGACA TCCCTCATCT CTCAGGCTGA GCAGTCACTG 
AAGGAGAACG ATCACGCTCG CTTCCTGCAA ACTGCTAAAA ACATCACTGA AAGGGTTTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATTA ATCTCAACGA TACTTTTGAT 
ACTTTCGCAC TTGATTTTAC CAGGGAGAAG AAATTGTTGG AATGCCTTGA TTATCTTACA 
GCTCCCAACC CTCCCACCAT TCGAGAAGAG CTCTGTACAG CTTCTTATGA TACTATTACT 
GTCCACTGGA CATCAGACGA TGAGTTCAGC GTGGTCTCTT ACGAGCTGCA GTACACCATC 
TTCACTGGAC AAGCTAACGT TGTTAGTTTA TGTAACTCAG CCGACAGCTG GATGATTGTT 
CCCAATATCA AACAAAACCA CTACACCGTG CATGGGTTAC AGAGTGGCAC AAAGTACATC 
TTCATTGTTA AGGCTATTAA TCAGGCTGGC AGCAGAAGCA GCGAGCCAGG CAAGCTCAAG 
ACAAACAGTC AGCCATTTAA ACTGGACCCC AAATCTGCTC ATAGAAAGCT GAAAGTGTCT 
CATGACAATT TGACAGTGGA ACGTGATGAA ACCTCCTCCA AAAAGAGTCA TACACCAGAG 
CGATTCACGA GCCAAGGGAG CTATGGAGTA GCTGGCAATG TGTTCATTGA CAGCGGACGG 
CATTACTGGG AAGTGGTTAT AAGTGGGAGT ACATGGTATG CCATTGGTAT TTCATACAAG 
TCAGCACCGA AGCATGAGTG GATCGGGAAG AACTCAGCCT CCTGGGTGCT GTGCCGCTGC 
AACAACACAT GGGTGGTGAG ACACAACAGC AAAGAAATCC CAATAGAGCC TGCGCCTCAT 
CTCCGCCGTG TCGGCATTTT GCTGGACTAC GACAATGGTT CCCTTGCTTT TTATGATGCC 
TTGAACTCCC TGCACCTTTA CACCTTTGAC ATTACGTTTG GGCAGCCCGT CTGCCCCACA 
TTCACTGTGT GGAATAAGTG TTTGACCATT ATAACAGGCT TGCCCATCCC TGATCACTTA 
GACTCCTCTG AGCAGCTGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of PP2A activity by Mid1 controls cranial neural crest speed and gangliogenesis.
Mechanisms of development128(11-12)560-76(2012 Jan-Feb)
Latta EJ,Golding JP

Evidence of functional redundancy between MID proteins: implications for the presentation of Opitz syndrome.
Developmental biology277(2)417-24(2005 Jan)
Granata A,Savery D,Hazan J,Cheung BM,Lumsden A,Quaderi NA

The Opitz syndrome gene MID1 is essential for establishing asymmetric gene expression in Hensen's node.
Developmental biology258(2)397-405(2003 Jun)
Granata A,Quaderi NA

Isolation and characterisation of the chick orthologue of the Opitz syndrome gene, Mid1, supports a conserved role in vertebrate development.
The International journal of developmental biology46(4)441-8(2002)
Richman JM,Fu KK,Cox LL,Sibbons JP,Cox TC

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MID1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報