MID1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_548857.6	XP_548857.2	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_014111838.2	XP_013967313.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_014111837.1	XP_013967312.1	E3 ubiquitin-protein ligase Midline-1
XM_005641098.3	XP_005641155.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_014111836.2	XP_013967311.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_014111836.1	XP_013967311.1	E3 ubiquitin-protein ligase Midline-1
XM_014111839.1	XP_013967314.1	E3 ubiquitin-protein ligase Midline-1
XM_548857.5	XP_548857.2	E3 ubiquitin-protein ligase Midline-1
XM_005641098.2	XP_005641155.1	E3 ubiquitin-protein ligase Midline-1
XM_014111838.1	XP_013967313.1	E3 ubiquitin-protein ligase Midline-1
XM_022415534.1	XP_022271242.1	E3 ubiquitin-protein ligase Midline-1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe32047	XM_005641098.3 Latest version!	Canis lupus familiaris midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32047	XM_014111838.2 Latest version!	Canis lupus familiaris midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32047	XM_014111837.1 Latest version!	Canis lupus familiaris midline 1 (MID1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32047	XM_548857.6 Latest version!	Canis lupus familiaris midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32047	XM_014111836.2 Latest version!	Canis lupus familiaris midline 1 (MID1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32047	XM_014111839.1 Latest version!	Canis lupus familiaris midline 1 (MID1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe285089	XM_022415534.1 Latest version!	Canis lupus familiaris midline 1 (MID1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe32047	XM_014111836.1	Canis lupus familiaris midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32047	XM_548857.5	Canis lupus familiaris midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32047	XM_005641098.2	Canis lupus familiaris midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32047	XM_014111838.1	Canis lupus familiaris midline 1 (MID1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

MID1 ( XM_548857.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_548857.2 Canis lupus familiaris midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_548857.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_548857.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548857.2
CDS	352..2355
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_548857.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548857.6

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_014111838.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967313.1 Canis lupus familiaris midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_014111838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967313.1
CDS	284..2287
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_014111838.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111838.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_014111837.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967312.1 Canis lupus familiaris midline 1 (MID1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_014111837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967312.1
CDS	274..2277
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_014111837.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111837.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005641098.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641155.1 Canis lupus familiaris midline 1 (MID1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_005641098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641155.1
CDS	206..2209
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005641098.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641098.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_014111836.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967311.1 Canis lupus familiaris midline 1 (MID1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_014111836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967311.1
CDS	255..2258
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_014111836.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111836.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_014111836.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967311.1 Canis lupus familiaris midline 1 (MID1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_014111836.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967311.1
CDS	444..2447
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_014111836.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111836.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_014111839.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967314.1 Canis lupus familiaris midline 1 (MID1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_014111839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967314.1
CDS	149..2152
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_014111839.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111839.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_548857.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_548857.2 Canis lupus familiaris midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_548857.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_548857.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548857.2
CDS	323..2326
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_548857.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548857.5

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005641098.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641155.1 Canis lupus familiaris midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_005641098.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641155.1
CDS	186..2189
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005641098.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641098.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_014111838.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967313.1 Canis lupus familiaris midline 1 (MID1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe32047
Clone ID Related Accession (Same CDS sequence)	XM_548857.6 , XM_014111838.2 , XM_014111837.1 , XM_005641098.3 , XM_014111836.2 , XM_014111836.1 , XM_014111839.1 , XM_548857.5 , XM_005641098.2 , XM_014111838.1
Accession Version	XM_014111838.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967313.1
CDS	168..2171
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_014111838.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111838.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAGCCTT ATCAAGAGGA ACACGGAATT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAGCAT GTGGAGGTCA ATGCATCACG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAGC AGTGCATCGA GCGGTCGGCA TCCCTCATCT CCCAGGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG CTTCCTACAG ACGGCTAAGA ACATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTTATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACTTTTGCCT TAGATTTTTC CCGAGAGAAG AAATTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCCTATGA CACCATCACC 
GTTCATTGGA CCTCCGATGA TGAATTCAGC GTGGTTTCCT ATGAGCTCCA ATACACCATA 
TTCACCGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCTG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACGGTC CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATCGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GCGAGCCCGG AAAGCTAAAG 
ACAAACAGCC AGCCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT GAAGGTGTCC 
CACGATAACT TAACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACGCCTGAA 
CGCTTCACCA GCCAAGGGAG CTACGGTGTA GCTGGAAATG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATCGGCCT TGCTTACAAG 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCGCT GTGCCGCTGT 
CACAATAACT GGGTAGTGAG ACACAACAGC AAGGAGACCC CCATCGAGCC GGCCCCCCAC 
CTCCGGCGCG TCGGCATCCT GCTGGACTAT GATAATGGCT CCATCGCCTT CTACGACGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC ATCACATTCT CGCAGCCTGT GTGCCCCACG 
TTCACGGTGT GGAACAAGTG CTTGACCATT ATAACTGGCC TCCCCATCCC AGACCATCTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_022415534.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271242.1 Canis lupus familiaris midline 1 (MID1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe285089
Clone ID Related Accession (Same CDS sequence)	XM_022415534.1
Accession Version	XM_022415534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AGCCTTATCA AGAGGAACAC GGAATTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAGCATGTGG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGATGGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAGCAGTG CATCGAGCGG TCGGCATCCC TCATCTCCCA GGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGCTTC CTACAGACGG CTAAGAACAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC ATCCCAGGTC CTTATTCCTG AAATCAACCT CAATGACACA 
TTTGACACTT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC CTATGACACC 
ATCACCGTTC ATTGGACCTC CGATGATGAA TTCAGCGTGG TTTCCTATGA GCTCCAATAC 
ACCATATTCA CCGGACAAGC CAATGTTGTT AGTCTGTGTA ACTCTGCCGA TAGCTGGATG 
ATCGTGCCCA ACATCAAGCA GAACCACTAC ACGGTCCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TCGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGCGA GCCCGGAAAG 
CTAAAGACAA ACAGCCAGCC ATTTAAACTG GACCCCAAAT CTGCACATCG AAAGCTGAAG 
GTGTCCCACG ATAACTTAAC AGTAGAACGT GATGAGTCAT CATCCAAAAA GAGTCACACG 
CCTGAACGCT TCACCAGCCA AGGGAGCTAC GGTGTAGCTG GAAATGTGTT CATTGATAGT 
GGCCGGCATT ACTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT CGGCCTTGCT 
TACAAGTCAG CCCCGAAGCA CGAGTGGATT GGGAAGAACT CTGCTTCCTG GGCGCTGTGC 
CGCTGTCACA ATAACTGGGT AGTGAGACAC AACAGCAAGG AGACCCCCAT CGAGCCGGCC 
CCCCACCTCC GGCGCGTCGG CATCCTGCTG GACTATGATA ATGGCTCCAT CGCCTTCTAC 
GACGCTTTGA ACTCCATCCA CCTCTACACC TTCGACATCA CATTCTCGCA GCCTGTGTGC 
CCCACGTTCA CGGTGTGGAA CAAGTGCTTG ACCATTATAA CTGGCCTCCC CATCCCAGAC 
CATCTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271242.1
CDS	353..2242
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKETPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_022415534.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 1 (MID1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415534.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGACTCA AGCGCAACGT CACCCTGCAG 
AACATCATCG ACAGGTTCCA GAAAGCGTCA GTGAGTGGGC CCAATTCCCC CAGCGAGACC 
CGCCGGGAGC GGGCGTTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAG 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCAAACAAGA AGCCTTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AGCCTTATCA AGAGGAACAC GGAATTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAGCATGTGG AGGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGATGGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAGCAGTG CATCGAGCGG TCGGCATCCC TCATCTCCCA GGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGCTTC CTACAGACGG CTAAGAACAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC ATCCCAGGTC CTTATTCCTG AAATCAACCT CAATGACACA 
TTTGACACTT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC CTATGACACC 
ATCACCGTTC ATTGGACCTC CGATGATGAA TTCAGCGTGG TTTCCTATGA GCTCCAATAC 
ACCATATTCA CCGGACAAGC CAATGTTGTT AGTCTGTGTA ACTCTGCCGA TAGCTGGATG 
ATCGTGCCCA ACATCAAGCA GAACCACTAC ACGGTCCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TCGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGCGA GCCCGGAAAG 
CTAAAGACAA ACAGCCAGCC ATTTAAACTG GACCCCAAAT CTGCACATCG AAAGCTGAAG 
GTGTCCCACG ATAACTTAAC AGTAGAACGT GATGAGTCAT CATCCAAAAA GAGTCACACG 
CCTGAACGCT TCACCAGCCA AGGGAGCTAC GGTGTAGCTG GAAATGTGTT CATTGATAGT 
GGCCGGCATT ACTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT CGGCCTTGCT 
TACAAGTCAG CCCCGAAGCA CGAGTGGATT GGGAAGAACT CTGCTTCCTG GGCGCTGTGC 
CGCTGTCACA ATAACTGGGT AGTGAGACAC AACAGCAAGG AGACCCCCAT CGAGCCGGCC 
CCCCACCTCC GGCGCGTCGG CATCCTGCTG GACTATGATA ATGGCTCCAT CGCCTTCTAC 
GACGCTTTGA ACTCCATCCA CCTCTACACC TTCGACATCA CATTCTCGCA GCCTGTGTGC 
CCCACGTTCA CGGTGTGGAA CAAGTGCTTG ACCATTATAA CTGGCCTCCC CATCCCAGAC 
CATCTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MID1
Entrez Gene ID	491737
Full Name	midline 1
General protein information	Preferred Names midline 1 Names E3 ubiquitin-protein ligase Midline-1 midline 1 (Opitz/BBB syndrome)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Mus musculus (house mouse)	Mid1	NP_034927.2
Homo sapiens (human)	MID1	NP_150632.1
Rattus norvegicus (Norway rat)	Mid1	NP_075216.1
Danio rerio (zebrafish)	mid1	XP_002663478.1
Xenopus tropicalis (tropical clawed frog)	mid1	NP_001006876.1
Bos taurus (cattle)	MID1	NP_001179751.1
Gallus gallus (chicken)	MID1	NP_989460.1
	MID1	XP_001093390.1
Canis lupus familiaris (dog)	MID1	XP_548857.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MID1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム