ROBO1 cDNA ORF clone, Bos taurus(cattle)

The following ROBO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa218842 XM_024990628.1
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa87635 XM_015468389.1
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa15492 NM_001192888.3
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15492 XM_005201165.4
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15494 XM_005201167.4
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15495 XM_005201168.3
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15496 XM_005201169.3
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15497 XM_005201170.4
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15498 XM_005201171.4
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15499 XM_005201172.4
Latest version!
Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa15492 XM_005201165.3 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15499 XM_005201172.3 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15498 XM_005201171.3 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15495 XM_005201168.2 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15497 XM_005201170.3 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15494 XM_005201167.3 Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa15492 NM_001192888.2 Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa218842
    Clone ID Related Accession (Same CDS sequence) XM_024990628.1
    Accession Version XM_024990628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT
    GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG
    GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGG GCAGCCCACC
    CCTTATGCCA CCACCCAGCT CATCCAGTCA AACCTCAGCA ACAACATGAA CAATGGCAGT
    GGAGACTCTG GCGAGAAGCA CTGGAAGCCA CCCGGACAGC AGAAACAGGA AGTGGCACCA
    ATTCAGTATA ACATCATGGA GCAAAACAAA CTGAACAAAG ATTATCGTGC AAATGACACA
    ATTGCTCCAA CTATCCCTTA CAACCAGTCA TATGACCAGA ATACTGGAGG ATCCTACAAC
    AGTTCAGACC GGGGAAGTAG TACATCAGGG AGTCAAGGGC ACAAGAAAGG GGCAAGGACA
    CCCAAAGTGC CAAAGCAAGG TGGCATGAAC TGGGCAGACC TACTGCCCCC TCCCCCAGCA
    CATCCTCCTC CTCATAGCAA CAGTGAAGAG TACAGCATCT CTGTAGATGA AAGCTATGAC
    CAAGAAATGC CATGCCCCGT GCCCCCAGCA CGGATGTATC TGCAACAAGG TGAACTAGAA
    GAGGAGGAAG ATGAACGAGG TCCCACTCCC CCTGTGCGGG GAGCCGCCTC TTCTCCGGCT
    GCCGTGTCTT ACAGCCATCA ATCCACCGCC ACTCTCACGC CCTCTCCACA GGAAGAACTC
    CAGCCCATGT TACAGGATTG TCCCGAGGAG GTGGGCCATG GGCAGCACCA GCCTGACAGG
    AGACGGCAGC CGGTGAGCCC ACCTCCACCT CCAAGGCCGA TTTCCCCACC CCACACCTAC
    GGCTACATTT CAGGACCCCT TGTCTCAGAT ATGGATACGG ATGCGCCAGA AGAGGAAGAA
    GACGAAGCCG ACATGGAAGT AGCCAAGATG CAGACCAGGA GGCTTTTGTT ACGTGGGCTG
    GAGCAGACCC CTGCCTCCAG TGTCGGGGAC CTGGAGAGCT CTGTCACCGG GTCCATGATC
    AATGGTTGGG GCTCTGCCTC AGAGGAGGAC AACATTTCCA GCGGACGCTC CAGCGTTAGT
    TCTTCTGATG GCTCTTTTTT CACTGATGCC GATTTTGCCC AGGCGGTCGC AGCAGCAGCG
    GAGTATGCTG GTCTGAAAGT GGCCCGGCGC CAAATGCAGG ATGCTGCTGG CCGCCGCCAT
    TTTCATGCAT CCCAGTGCCC TAGACCCACA AGTCCTGTTT CTACAGACAG CAACGTGAGT
    GCTGCTGTAG TACAGAAGGC CAGACCAGCC AAGAAACAAA AACACCAGCC AGGACATCTG
    CGCAGAGAAG CCTACACAGA TGATCTTCCA CCTCCCCCTG TGCCACCACC TGCTATCAAG
    TCACCCACTG CCCAGTCCAA GGCCCAGCTG GAGGTTCGAC CCGTGATGGT GCCAAAACTC
    CCTTCTATAG AAGCAAGAAC AGACAGATCA TCAGATAGGA AAGGAGGCAA TTACAAGGGG
    AGAGAAGTAT TGGATGGAAG ACAAGTTGCT GATATGCGAC CAAATCCAGG TGATGCCAGA
    GAAGCACAGG AACAGCCAAA TGACGGGAAA GGACGAGGAA ACAAGGCAGC AAAACGAGAC
    CTTCCACCAG CAAAGGCTCA TCTCATCCAA GAGGATATTC TGCCTTACTG TAGACCTACT
    TTTCCGACAT CAAATAATCC CAGAGATCCT AGTTCTTCAA GTTCTATGTC ATCAAGAGGA
    TCAGGAAGCA GACAAAGAGA ACAAGCAAAT GTAGGTCGAA GAAATATTGC AGAGATGCAA
    GTTCTTGGAG GATATGAAAG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846396.1
    CDS1112..5791
    Translation

    Target ORF information:

    RefSeq Version XM_024990628.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT
    GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG
    GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGG GCAGCCCACC
    CCTTATGCCA CCACCCAGCT CATCCAGTCA AACCTCAGCA ACAACATGAA CAATGGCAGT
    GGAGACTCTG GCGAGAAGCA CTGGAAGCCA CCCGGACAGC AGAAACAGGA AGTGGCACCA
    ATTCAGTATA ACATCATGGA GCAAAACAAA CTGAACAAAG ATTATCGTGC AAATGACACA
    ATTGCTCCAA CTATCCCTTA CAACCAGTCA TATGACCAGA ATACTGGAGG ATCCTACAAC
    AGTTCAGACC GGGGAAGTAG TACATCAGGG AGTCAAGGGC ACAAGAAAGG GGCAAGGACA
    CCCAAAGTGC CAAAGCAAGG TGGCATGAAC TGGGCAGACC TACTGCCCCC TCCCCCAGCA
    CATCCTCCTC CTCATAGCAA CAGTGAAGAG TACAGCATCT CTGTAGATGA AAGCTATGAC
    CAAGAAATGC CATGCCCCGT GCCCCCAGCA CGGATGTATC TGCAACAAGG TGAACTAGAA
    GAGGAGGAAG ATGAACGAGG TCCCACTCCC CCTGTGCGGG GAGCCGCCTC TTCTCCGGCT
    GCCGTGTCTT ACAGCCATCA ATCCACCGCC ACTCTCACGC CCTCTCCACA GGAAGAACTC
    CAGCCCATGT TACAGGATTG TCCCGAGGAG GTGGGCCATG GGCAGCACCA GCCTGACAGG
    AGACGGCAGC CGGTGAGCCC ACCTCCACCT CCAAGGCCGA TTTCCCCACC CCACACCTAC
    GGCTACATTT CAGGACCCCT TGTCTCAGAT ATGGATACGG ATGCGCCAGA AGAGGAAGAA
    GACGAAGCCG ACATGGAAGT AGCCAAGATG CAGACCAGGA GGCTTTTGTT ACGTGGGCTG
    GAGCAGACCC CTGCCTCCAG TGTCGGGGAC CTGGAGAGCT CTGTCACCGG GTCCATGATC
    AATGGTTGGG GCTCTGCCTC AGAGGAGGAC AACATTTCCA GCGGACGCTC CAGCGTTAGT
    TCTTCTGATG GCTCTTTTTT CACTGATGCC GATTTTGCCC AGGCGGTCGC AGCAGCAGCG
    GAGTATGCTG GTCTGAAAGT GGCCCGGCGC CAAATGCAGG ATGCTGCTGG CCGCCGCCAT
    TTTCATGCAT CCCAGTGCCC TAGACCCACA AGTCCTGTTT CTACAGACAG CAACGTGAGT
    GCTGCTGTAG TACAGAAGGC CAGACCAGCC AAGAAACAAA AACACCAGCC AGGACATCTG
    CGCAGAGAAG CCTACACAGA TGATCTTCCA CCTCCCCCTG TGCCACCACC TGCTATCAAG
    TCACCCACTG CCCAGTCCAA GGCCCAGCTG GAGGTTCGAC CCGTGATGGT GCCAAAACTC
    CCTTCTATAG AAGCAAGAAC AGACAGATCA TCAGATAGGA AAGGAGGCAA TTACAAGGGG
    AGAGAAGTAT TGGATGGAAG ACAAGTTGCT GATATGCGAC CAAATCCAGG TGATGCCAGA
    GAAGCACAGG AACAGCCAAA TGACGGGAAA GGACGAGGAA ACAAGGCAGC AAAACGAGAC
    CTTCCACCAG CAAAGGCTCA TCTCATCCAA GAGGATATTC TGCCTTACTG TAGACCTACT
    TTTCCGACAT CAAATAATCC CAGAGATCCT AGTTCTTCAA GTTCTATGTC ATCAAGAGGA
    TCAGGAAGCA GACAAAGAGA ACAAGCAAAT GTAGGTCGAA GAAATATTGC AGAGATGCAA
    GTTCTTGGAG GATATGAAAG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87635
    Clone ID Related Accession (Same CDS sequence) XM_015468389.1
    Accession Version XM_015468389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG
    GTGGTTGCCT TGGGACGAAC GGTCACTTTC CAGTGTGAAG CAACAGGAAA TCCCCAACCA
    GCTATCTTCT GGAGGAGAGA GGGGAGTCAG AATCTGCTTT TCTCATATCA GCCACCACAG
    TCATCCAGCC GTTTTTCAGT CTCCCAGACT GGTGACCTCA CTATTACTAA TGTCCAGCGA
    TCTGATGTTG GTTATTATAT CTGCCAGACT TTGAATGTTG CCGGAAGTAT CATCACAAAA
    GCATATTTGG AAGTTACCGA TGTGATTGCA GACCGACCTC CCCCAGTCAT TCGACAAGGT
    CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC
    AGTCCAGTGC CCACAATTCT GTGGAGAAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT
    CGAATCAAAC AGCTGGAGAC CGGAGTACTA CAGATCCGAT ATGCTAAGCT GGGTGATACT
    GGTCGATACA CGTGCATTGC ATCAACCCCC AGTGGTGAAG CAACGTGGAG TGCTTACATT
    GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CCACCGACCC AAATTTAATC
    CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCC GAAATACAGT AACGTTGTCA
    TGGCAACCAA ATTTGAATTC AGGTGCAACT CCGACGTCTT ATATTATAGA AGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAGACTGTA GCGGAGAATG TGAAAACAGA AACATTTGCC
    ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC CAATGCATAT
    GGAATTAGTG ATCCAAGCCA GATATCAGAC CCAGTGAAAA CACAAGATGT TCCCCCAACC
    AGTCAGGGGG TGGATCACAA GCAGGTCCAG CGAGAACTGG GAAATGTTGT ACTGCATCTC
    CACAACCCCA CCATCCTTTC CTCCTCTTCA ATCGAAGTGC ACTGGACAGT GGACCAACAG
    TCTCAGTATA TTCAAGGATA CAAAGTTCTT TACCGCCCGT CTGGAGCCAA CCATGGTGAA
    TCCGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ACAGTGTGGT TATCCCTGAT
    CTCAAGAAAG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA
    GCAGATAGTG AAATTAAATT TGCCAAAACC CTAGAAGAAG CACCCAGTGC CCCACCTCAA
    AGTGTGACTG TATCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGCCA
    CCTCCTGAAG ATACTCAAAA TGGAATGGTC CAAGAGTATA AGGTCTGGTG TCTGGGCAAT
    GAAACGCGAT ACCATATAAA CAAGACAGTA GATGGTTCCA CCTTTTCTGT GGTTATTCCC
    TCACTGGTTC CTGGGATCCG ATATAGTGTA GAGGTGGCAG CCAGCACGGG GGCTGGGTCG
    GGGGTGAAGA GTGAGCCACA ATTCATCCAG TTAGATTCTC ATGGAAACCC CGTGTCACCT
    GAGGACCAAG TCAGCCTTGC TCAGCAGATC TCAGACGTAG TGAAGCAGCC GGCATTCATC
    GCAGGAATCG GGGCTGCCTG TTGGATCATC CTTATGGTTT TTAGCATCTG GCTCTATCGA
    CACCGAAAGA AGAGAAATGG GCTGGCTAGT ACTTATGCGG GTATCAGAAA AGTCCCGTCT
    TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGAGGCG AAGCTGTGAG CAGTGGAGGG
    AGGCCAGGAC TTCTCAACAT CAGTGAACCT GCCACACAGC CATGGCTTGC AGACACATGG
    CCTAACACTG GCAACAATCA CAATGACTGC TCCATCAATT GCTGCACGGC AGGCAATGGG
    AACAGTGACA GCAACTTGAC CACGTATAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC
    AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT
    GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT
    GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG
    CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG
    TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA
    GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG
    AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA
    GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA
    GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT
    CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC
    GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG
    GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA
    CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA
    GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG
    ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG
    GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG
    GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT
    GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG
    CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC
    ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA
    GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT
    CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG
    CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA
    TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT
    GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG
    AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC
    CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323875.1
    CDS297..5249
    Translation

    Target ORF information:

    RefSeq Version XM_015468389.1
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG
    GTGGTTGCCT TGGGACGAAC GGTCACTTTC CAGTGTGAAG CAACAGGAAA TCCCCAACCA
    GCTATCTTCT GGAGGAGAGA GGGGAGTCAG AATCTGCTTT TCTCATATCA GCCACCACAG
    TCATCCAGCC GTTTTTCAGT CTCCCAGACT GGTGACCTCA CTATTACTAA TGTCCAGCGA
    TCTGATGTTG GTTATTATAT CTGCCAGACT TTGAATGTTG CCGGAAGTAT CATCACAAAA
    GCATATTTGG AAGTTACCGA TGTGATTGCA GACCGACCTC CCCCAGTCAT TCGACAAGGT
    CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC
    AGTCCAGTGC CCACAATTCT GTGGAGAAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT
    CGAATCAAAC AGCTGGAGAC CGGAGTACTA CAGATCCGAT ATGCTAAGCT GGGTGATACT
    GGTCGATACA CGTGCATTGC ATCAACCCCC AGTGGTGAAG CAACGTGGAG TGCTTACATT
    GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CCACCGACCC AAATTTAATC
    CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCC GAAATACAGT AACGTTGTCA
    TGGCAACCAA ATTTGAATTC AGGTGCAACT CCGACGTCTT ATATTATAGA AGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAGACTGTA GCGGAGAATG TGAAAACAGA AACATTTGCC
    ATTAAAGGAC TCAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGGGCAGC CAATGCATAT
    GGAATTAGTG ATCCAAGCCA GATATCAGAC CCAGTGAAAA CACAAGATGT TCCCCCAACC
    AGTCAGGGGG TGGATCACAA GCAGGTCCAG CGAGAACTGG GAAATGTTGT ACTGCATCTC
    CACAACCCCA CCATCCTTTC CTCCTCTTCA ATCGAAGTGC ACTGGACAGT GGACCAACAG
    TCTCAGTATA TTCAAGGATA CAAAGTTCTT TACCGCCCGT CTGGAGCCAA CCATGGTGAA
    TCCGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ACAGTGTGGT TATCCCTGAT
    CTCAAGAAAG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA
    GCAGATAGTG AAATTAAATT TGCCAAAACC CTAGAAGAAG CACCCAGTGC CCCACCTCAA
    AGTGTGACTG TATCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGCCA
    CCTCCTGAAG ATACTCAAAA TGGAATGGTC CAAGAGTATA AGGTCTGGTG TCTGGGCAAT
    GAAACGCGAT ACCATATAAA CAAGACAGTA GATGGTTCCA CCTTTTCTGT GGTTATTCCC
    TCACTGGTTC CTGGGATCCG ATATAGTGTA GAGGTGGCAG CCAGCACGGG GGCTGGGTCG
    GGGGTGAAGA GTGAGCCACA ATTCATCCAG TTAGATTCTC ATGGAAACCC CGTGTCACCT
    GAGGACCAAG TCAGCCTTGC TCAGCAGATC TCAGACGTAG TGAAGCAGCC GGCATTCATC
    GCAGGAATCG GGGCTGCCTG TTGGATCATC CTTATGGTTT TTAGCATCTG GCTCTATCGA
    CACCGAAAGA AGAGAAATGG GCTGGCTAGT ACTTATGCGG GTATCAGAAA AGTCCCGTCT
    TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGAGGCG AAGCTGTGAG CAGTGGAGGG
    AGGCCAGGAC TTCTCAACAT CAGTGAACCT GCCACACAGC CATGGCTTGC AGACACATGG
    CCTAACACTG GCAACAATCA CAATGACTGC TCCATCAATT GCTGCACGGC AGGCAATGGG
    AACAGTGACA GCAACTTGAC CACGTATAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC
    AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT
    GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT
    GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG
    CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG
    TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA
    GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG
    AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA
    GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA
    GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT
    CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC
    GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG
    GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA
    CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA
    GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG
    ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG
    GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG
    GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT
    GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG
    CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC
    ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA
    GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT
    CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG
    CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA
    TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT
    GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG
    AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC
    CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15492
    Clone ID Related Accession (Same CDS sequence) NM_001192888.2 , XM_005201165.4 , XM_005201165.3 , NM_001192888.3
    Accession Version NM_001192888.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02000642.1, DAAA02000605.1, DAAA02000603.1, DAAA02000593.1, DAAA02000592.1 and DAAA02000591.1. On Apr 26, 2011 this sequence version replaced NM_001192888.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822095, SAMN04019640 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG
    CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT
    TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT
    AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA
    GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC
    AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC
    GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA
    GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT
    CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT
    TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC
    CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC
    CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA
    GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC
    AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC
    TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT
    TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA
    GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC
    CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG
    GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA
    GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC
    AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA
    AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA
    AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT
    CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA
    AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179817.2
    CDS52..5013
    Translation

    Target ORF information:

    RefSeq Version NM_001192888.2
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192888.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG
    CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT
    TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT
    AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA
    GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC
    AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC
    GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA
    GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT
    CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT
    TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC
    CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC
    CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA
    GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC
    AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC
    TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT
    TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA
    GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC
    CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG
    GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA
    GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC
    AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA
    AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA
    AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT
    CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA
    AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15492
    Clone ID Related Accession (Same CDS sequence) NM_001192888.2 , XM_005201165.4 , XM_005201165.3 , NM_001192888.3
    Accession Version NM_001192888.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-15
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Jun 16, 2018 this sequence version replaced NM_001192888.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG
    CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT
    TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT
    AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA
    GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC
    AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC
    GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA
    GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT
    CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT
    TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC
    CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC
    CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA
    GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC
    AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC
    TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT
    TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA
    GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC
    CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG
    GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA
    GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC
    AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA
    AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA
    AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT
    CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA
    AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179817.2
    CDS52..5013
    Translation

    Target ORF information:

    RefSeq Version NM_001192888.3
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192888.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG
    CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT
    TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT
    AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA
    GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC
    AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC
    GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA
    GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT
    CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT
    TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC
    CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC
    CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA
    GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC
    AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC
    TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT
    TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA
    GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC
    CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG
    GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA
    GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC
    AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA
    AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA
    AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT
    CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA
    AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15492
    Clone ID Related Accession (Same CDS sequence) NM_001192888.2 , XM_005201165.4 , XM_005201165.3 , NM_001192888.3
    Accession Version XM_005201165.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201165.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG
    CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT
    TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT
    AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA
    GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC
    AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC
    GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA
    GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT
    CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT
    TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC
    CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC
    CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA
    GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC
    AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC
    TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT
    TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA
    GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC
    CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG
    GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA
    GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC
    AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA
    AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA
    AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT
    CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA
    AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201222.1
    CDS297..5258
    Translation

    Target ORF information:

    RefSeq Version XM_005201165.4
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201165.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG
    CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT
    TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT
    AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA
    GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC
    AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC
    GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA
    GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT
    CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT
    TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC
    CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC
    CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA
    GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC
    AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC
    TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT
    TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA
    GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC
    CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG
    GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA
    GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC
    AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA
    AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA
    AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT
    CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA
    AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15492
    Clone ID Related Accession (Same CDS sequence) NM_001192888.2 , XM_005201165.4 , XM_005201165.3 , NM_001192888.3
    Accession Version XM_005201165.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG
    CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT
    TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT
    AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA
    GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC
    AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC
    GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA
    GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT
    CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT
    TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC
    CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC
    CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA
    GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC
    AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC
    TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT
    TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA
    GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC
    CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG
    GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA
    GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC
    AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA
    AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA
    AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT
    CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA
    AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201222.1
    CDS297..5258
    Translation

    Target ORF information:

    RefSeq Version XM_005201165.3
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201165.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGATG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CCCCTTATGC CACCACCCAG
    CTCATCCAGT CAAACCTCAG CAACAACATG AACAATGGCA GTGGAGACTC TGGCGAGAAG
    CACTGGAAGC CACCCGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AACTGAACAA AGATTATCGT GCAAATGACA CAATTGCTCC AACTATCCCT
    TACAACCAGT CATATGACCA GAATACTGGA GGATCCTACA ACAGTTCAGA CCGGGGAAGT
    AGTACATCAG GGAGTCAAGG GCACAAGAAA GGGGCAAGGA CACCCAAAGT GCCAAAGCAA
    GGTGGCATGA ACTGGGCAGA CCTACTGCCC CCTCCCCCAG CACATCCTCC TCCTCATAGC
    AACAGTGAAG AGTACAGCAT CTCTGTAGAT GAAAGCTATG ACCAAGAAAT GCCATGCCCC
    GTGCCCCCAG CACGGATGTA TCTGCAACAA GGTGAACTAG AAGAGGAGGA AGATGAACGA
    GGTCCCACTC CCCCTGTGCG GGGAGCCGCC TCTTCTCCGG CTGCCGTGTC TTACAGCCAT
    CAATCCACCG CCACTCTCAC GCCCTCTCCA CAGGAAGAAC TCCAGCCCAT GTTACAGGAT
    TGTCCCGAGG AGGTGGGCCA TGGGCAGCAC CAGCCTGACA GGAGACGGCA GCCGGTGAGC
    CCACCTCCAC CTCCAAGGCC GATTTCCCCA CCCCACACCT ACGGCTACAT TTCAGGACCC
    CTTGTCTCAG ATATGGATAC GGATGCGCCA GAAGAGGAAG AAGACGAAGC CGACATGGAA
    GTAGCCAAGA TGCAGACCAG GAGGCTTTTG TTACGTGGGC TGGAGCAGAC CCCTGCCTCC
    AGTGTCGGGG ACCTGGAGAG CTCTGTCACC GGGTCCATGA TCAATGGTTG GGGCTCTGCC
    TCAGAGGAGG ACAACATTTC CAGCGGACGC TCCAGCGTTA GTTCTTCTGA TGGCTCTTTT
    TTCACTGATG CCGATTTTGC CCAGGCGGTC GCAGCAGCAG CGGAGTATGC TGGTCTGAAA
    GTGGCCCGGC GCCAAATGCA GGATGCTGCT GGCCGCCGCC ATTTTCATGC ATCCCAGTGC
    CCTAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACGTGA GTGCTGCTGT AGTACAGAAG
    GCCAGACCAG CCAAGAAACA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA
    GATGATCTTC CACCTCCCCC TGTGCCACCA CCTGCTATCA AGTCACCCAC TGCCCAGTCC
    AAGGCCCAGC TGGAGGTTCG ACCCGTGATG GTGCCAAAAC TCCCTTCTAT AGAAGCAAGA
    ACAGACAGAT CATCAGATAG GAAAGGAGGC AATTACAAGG GGAGAGAAGT ATTGGATGGA
    AGACAAGTTG CTGATATGCG ACCAAATCCA GGTGATGCCA GAGAAGCACA GGAACAGCCA
    AATGACGGGA AAGGACGAGG AAACAAGGCA GCAAAACGAG ACCTTCCACC AGCAAAGGCT
    CATCTCATCC AAGAGGATAT TCTGCCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT
    CCCAGAGATC CTAGTTCTTC AAGTTCTATG TCATCAAGAG GATCAGGAAG CAGACAAAGA
    GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAGATGC AAGTTCTTGG AGGATATGAA
    AGAGAAGATA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15494
    Clone ID Related Accession (Same CDS sequence) XM_005201167.3 , XM_005201167.4
    Accession Version XM_005201167.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTAACTTATC AGAGAGGAGG CGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC
    ATCAGTGAAC CTGCCACACA GCCATGGCTT GCAGACACAT GGCCTAACAC TGGCAACAAT
    CACAATGACT GCTCCATCAA TTGCTGCACG GCAGGCAATG GGAACAGTGA CAGCAACTTG
    ACCACGTATA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA
    CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA
    TCAGGGCAGC CCACCCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC
    ATGAACAATG GCAGTGGAGA CTCTGGCGAG AAGCACTGGA AGCCACCCGG ACAGCAGAAA
    CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT
    CGTGCAAATG ACACAATTGC TCCAACTATC CCTTACAACC AGTCATATGA CCAGAATACT
    GGAGGATCCT ACAACAGTTC AGACCGGGGA AGTAGTACAT CAGGGAGTCA AGGGCACAAG
    AAAGGGGCAA GGACACCCAA AGTGCCAAAG CAAGGTGGCA TGAACTGGGC AGACCTACTG
    CCCCCTCCCC CAGCACATCC TCCTCCTCAT AGCAACAGTG AAGAGTACAG CATCTCTGTA
    GATGAAAGCT ATGACCAAGA AATGCCATGC CCCGTGCCCC CAGCACGGAT GTATCTGCAA
    CAAGGTGAAC TAGAAGAGGA GGAAGATGAA CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC
    GCCTCTTCTC CGGCTGCCGT GTCTTACAGC CATCAATCCA CCGCCACTCT CACGCCCTCT
    CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCCG AGGAGGTGGG CCATGGGCAG
    CACCAGCCTG ACAGGAGACG GCAGCCGGTG AGCCCACCTC CACCTCCAAG GCCGATTTCC
    CCACCCCACA CCTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACGGATGCG
    CCAGAAGAGG AAGAAGACGA AGCCGACATG GAAGTAGCCA AGATGCAGAC CAGGAGGCTT
    TTGTTACGTG GGCTGGAGCA GACCCCTGCC TCCAGTGTCG GGGACCTGGA GAGCTCTGTC
    ACCGGGTCCA TGATCAATGG TTGGGGCTCT GCCTCAGAGG AGGACAACAT TTCCAGCGGA
    CGCTCCAGCG TTAGTTCTTC TGATGGCTCT TTTTTCACTG ATGCCGATTT TGCCCAGGCG
    GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG AAAGTGGCCC GGCGCCAAAT GCAGGATGCT
    GCTGGCCGCC GCCATTTTCA TGCATCCCAG TGCCCTAGAC CCACAAGTCC TGTTTCTACA
    GACAGCAACG TGAGTGCTGC TGTAGTACAG AAGGCCAGAC CAGCCAAGAA ACAAAAACAC
    CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC CCCTGTGCCA
    CCACCTGCTA TCAAGTCACC CACTGCCCAG TCCAAGGCCC AGCTGGAGGT TCGACCCGTG
    ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACA GATCATCAGA TAGGAAAGGA
    GGCAATTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCTGATAT GCGACCAAAT
    CCAGGTGATG CCAGAGAAGC ACAGGAACAG CCAAATGACG GGAAAGGACG AGGAAACAAG
    GCAGCAAAAC GAGACCTTCC ACCAGCAAAG GCTCATCTCA TCCAAGAGGA TATTCTGCCT
    TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGTTCT
    ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT
    ATTGCAGAGA TGCAAGTTCT TGGAGGATAT GAAAGAGAAG ATAATAATGA AGAATTAGAG
    GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201224.1
    CDS297..5231
    Translation

    Target ORF information:

    RefSeq Version XM_005201167.3
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201167.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTAACTTATC AGAGAGGAGG CGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC
    ATCAGTGAAC CTGCCACACA GCCATGGCTT GCAGACACAT GGCCTAACAC TGGCAACAAT
    CACAATGACT GCTCCATCAA TTGCTGCACG GCAGGCAATG GGAACAGTGA CAGCAACTTG
    ACCACGTATA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA
    CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA
    TCAGGGCAGC CCACCCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC
    ATGAACAATG GCAGTGGAGA CTCTGGCGAG AAGCACTGGA AGCCACCCGG ACAGCAGAAA
    CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT
    CGTGCAAATG ACACAATTGC TCCAACTATC CCTTACAACC AGTCATATGA CCAGAATACT
    GGAGGATCCT ACAACAGTTC AGACCGGGGA AGTAGTACAT CAGGGAGTCA AGGGCACAAG
    AAAGGGGCAA GGACACCCAA AGTGCCAAAG CAAGGTGGCA TGAACTGGGC AGACCTACTG
    CCCCCTCCCC CAGCACATCC TCCTCCTCAT AGCAACAGTG AAGAGTACAG CATCTCTGTA
    GATGAAAGCT ATGACCAAGA AATGCCATGC CCCGTGCCCC CAGCACGGAT GTATCTGCAA
    CAAGGTGAAC TAGAAGAGGA GGAAGATGAA CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC
    GCCTCTTCTC CGGCTGCCGT GTCTTACAGC CATCAATCCA CCGCCACTCT CACGCCCTCT
    CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCCG AGGAGGTGGG CCATGGGCAG
    CACCAGCCTG ACAGGAGACG GCAGCCGGTG AGCCCACCTC CACCTCCAAG GCCGATTTCC
    CCACCCCACA CCTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACGGATGCG
    CCAGAAGAGG AAGAAGACGA AGCCGACATG GAAGTAGCCA AGATGCAGAC CAGGAGGCTT
    TTGTTACGTG GGCTGGAGCA GACCCCTGCC TCCAGTGTCG GGGACCTGGA GAGCTCTGTC
    ACCGGGTCCA TGATCAATGG TTGGGGCTCT GCCTCAGAGG AGGACAACAT TTCCAGCGGA
    CGCTCCAGCG TTAGTTCTTC TGATGGCTCT TTTTTCACTG ATGCCGATTT TGCCCAGGCG
    GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG AAAGTGGCCC GGCGCCAAAT GCAGGATGCT
    GCTGGCCGCC GCCATTTTCA TGCATCCCAG TGCCCTAGAC CCACAAGTCC TGTTTCTACA
    GACAGCAACG TGAGTGCTGC TGTAGTACAG AAGGCCAGAC CAGCCAAGAA ACAAAAACAC
    CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC CCCTGTGCCA
    CCACCTGCTA TCAAGTCACC CACTGCCCAG TCCAAGGCCC AGCTGGAGGT TCGACCCGTG
    ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACA GATCATCAGA TAGGAAAGGA
    GGCAATTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCTGATAT GCGACCAAAT
    CCAGGTGATG CCAGAGAAGC ACAGGAACAG CCAAATGACG GGAAAGGACG AGGAAACAAG
    GCAGCAAAAC GAGACCTTCC ACCAGCAAAG GCTCATCTCA TCCAAGAGGA TATTCTGCCT
    TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGTTCT
    ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT
    ATTGCAGAGA TGCAAGTTCT TGGAGGATAT GAAAGAGAAG ATAATAATGA AGAATTAGAG
    GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15494
    Clone ID Related Accession (Same CDS sequence) XM_005201167.3 , XM_005201167.4
    Accession Version XM_005201167.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201167.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTAACTTATC AGAGAGGAGG CGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC
    ATCAGTGAAC CTGCCACACA GCCATGGCTT GCAGACACAT GGCCTAACAC TGGCAACAAT
    CACAATGACT GCTCCATCAA TTGCTGCACG GCAGGCAATG GGAACAGTGA CAGCAACTTG
    ACCACGTATA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA
    CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA
    TCAGGGCAGC CCACCCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC
    ATGAACAATG GCAGTGGAGA CTCTGGCGAG AAGCACTGGA AGCCACCCGG ACAGCAGAAA
    CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT
    CGTGCAAATG ACACAATTGC TCCAACTATC CCTTACAACC AGTCATATGA CCAGAATACT
    GGAGGATCCT ACAACAGTTC AGACCGGGGA AGTAGTACAT CAGGGAGTCA AGGGCACAAG
    AAAGGGGCAA GGACACCCAA AGTGCCAAAG CAAGGTGGCA TGAACTGGGC AGACCTACTG
    CCCCCTCCCC CAGCACATCC TCCTCCTCAT AGCAACAGTG AAGAGTACAG CATCTCTGTA
    GATGAAAGCT ATGACCAAGA AATGCCATGC CCCGTGCCCC CAGCACGGAT GTATCTGCAA
    CAAGGTGAAC TAGAAGAGGA GGAAGATGAA CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC
    GCCTCTTCTC CGGCTGCCGT GTCTTACAGC CATCAATCCA CCGCCACTCT CACGCCCTCT
    CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCCG AGGAGGTGGG CCATGGGCAG
    CACCAGCCTG ACAGGAGACG GCAGCCGGTG AGCCCACCTC CACCTCCAAG GCCGATTTCC
    CCACCCCACA CCTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACGGATGCG
    CCAGAAGAGG AAGAAGACGA AGCCGACATG GAAGTAGCCA AGATGCAGAC CAGGAGGCTT
    TTGTTACGTG GGCTGGAGCA GACCCCTGCC TCCAGTGTCG GGGACCTGGA GAGCTCTGTC
    ACCGGGTCCA TGATCAATGG TTGGGGCTCT GCCTCAGAGG AGGACAACAT TTCCAGCGGA
    CGCTCCAGCG TTAGTTCTTC TGATGGCTCT TTTTTCACTG ATGCCGATTT TGCCCAGGCG
    GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG AAAGTGGCCC GGCGCCAAAT GCAGGATGCT
    GCTGGCCGCC GCCATTTTCA TGCATCCCAG TGCCCTAGAC CCACAAGTCC TGTTTCTACA
    GACAGCAACG TGAGTGCTGC TGTAGTACAG AAGGCCAGAC CAGCCAAGAA ACAAAAACAC
    CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC CCCTGTGCCA
    CCACCTGCTA TCAAGTCACC CACTGCCCAG TCCAAGGCCC AGCTGGAGGT TCGACCCGTG
    ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACA GATCATCAGA TAGGAAAGGA
    GGCAATTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCTGATAT GCGACCAAAT
    CCAGGTGATG CCAGAGAAGC ACAGGAACAG CCAAATGACG GGAAAGGACG AGGAAACAAG
    GCAGCAAAAC GAGACCTTCC ACCAGCAAAG GCTCATCTCA TCCAAGAGGA TATTCTGCCT
    TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGTTCT
    ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT
    ATTGCAGAGA TGCAAGTTCT TGGAGGATAT GAAAGAGAAG ATAATAATGA AGAATTAGAG
    GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201224.1
    CDS297..5231
    Translation

    Target ORF information:

    RefSeq Version XM_005201167.4
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201167.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTAACTTATC AGAGAGGAGG CGAAGCTGTG AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC
    ATCAGTGAAC CTGCCACACA GCCATGGCTT GCAGACACAT GGCCTAACAC TGGCAACAAT
    CACAATGACT GCTCCATCAA TTGCTGCACG GCAGGCAATG GGAACAGTGA CAGCAACTTG
    ACCACGTATA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA
    CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ATGGGCGTTT TGTCAATCCA
    TCAGGGCAGC CCACCCCTTA TGCCACCACC CAGCTCATCC AGTCAAACCT CAGCAACAAC
    ATGAACAATG GCAGTGGAGA CTCTGGCGAG AAGCACTGGA AGCCACCCGG ACAGCAGAAA
    CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT
    CGTGCAAATG ACACAATTGC TCCAACTATC CCTTACAACC AGTCATATGA CCAGAATACT
    GGAGGATCCT ACAACAGTTC AGACCGGGGA AGTAGTACAT CAGGGAGTCA AGGGCACAAG
    AAAGGGGCAA GGACACCCAA AGTGCCAAAG CAAGGTGGCA TGAACTGGGC AGACCTACTG
    CCCCCTCCCC CAGCACATCC TCCTCCTCAT AGCAACAGTG AAGAGTACAG CATCTCTGTA
    GATGAAAGCT ATGACCAAGA AATGCCATGC CCCGTGCCCC CAGCACGGAT GTATCTGCAA
    CAAGGTGAAC TAGAAGAGGA GGAAGATGAA CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC
    GCCTCTTCTC CGGCTGCCGT GTCTTACAGC CATCAATCCA CCGCCACTCT CACGCCCTCT
    CCACAGGAAG AACTCCAGCC CATGTTACAG GATTGTCCCG AGGAGGTGGG CCATGGGCAG
    CACCAGCCTG ACAGGAGACG GCAGCCGGTG AGCCCACCTC CACCTCCAAG GCCGATTTCC
    CCACCCCACA CCTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACGGATGCG
    CCAGAAGAGG AAGAAGACGA AGCCGACATG GAAGTAGCCA AGATGCAGAC CAGGAGGCTT
    TTGTTACGTG GGCTGGAGCA GACCCCTGCC TCCAGTGTCG GGGACCTGGA GAGCTCTGTC
    ACCGGGTCCA TGATCAATGG TTGGGGCTCT GCCTCAGAGG AGGACAACAT TTCCAGCGGA
    CGCTCCAGCG TTAGTTCTTC TGATGGCTCT TTTTTCACTG ATGCCGATTT TGCCCAGGCG
    GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG AAAGTGGCCC GGCGCCAAAT GCAGGATGCT
    GCTGGCCGCC GCCATTTTCA TGCATCCCAG TGCCCTAGAC CCACAAGTCC TGTTTCTACA
    GACAGCAACG TGAGTGCTGC TGTAGTACAG AAGGCCAGAC CAGCCAAGAA ACAAAAACAC
    CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC CCCTGTGCCA
    CCACCTGCTA TCAAGTCACC CACTGCCCAG TCCAAGGCCC AGCTGGAGGT TCGACCCGTG
    ATGGTGCCAA AACTCCCTTC TATAGAAGCA AGAACAGACA GATCATCAGA TAGGAAAGGA
    GGCAATTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCTGATAT GCGACCAAAT
    CCAGGTGATG CCAGAGAAGC ACAGGAACAG CCAAATGACG GGAAAGGACG AGGAAACAAG
    GCAGCAAAAC GAGACCTTCC ACCAGCAAAG GCTCATCTCA TCCAAGAGGA TATTCTGCCT
    TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGTTCT
    ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT
    ATTGCAGAGA TGCAAGTTCT TGGAGGATAT GAAAGAGAAG ATAATAATGA AGAATTAGAG
    GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15495
    Clone ID Related Accession (Same CDS sequence) XM_005201168.3 , XM_005201168.2
    Accession Version XM_005201168.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201168.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT
    GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG
    GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGC TGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACCCCTTA TGCCACCACC
    CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGTGGAGA CTCTGGCGAG
    AAGCACTGGA AGCCACCCGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGTGCAAATG ACACAATTGC TCCAACTATC
    CCTTACAACC AGTCATATGA CCAGAATACT GGAGGATCCT ACAACAGTTC AGACCGGGGA
    AGTAGTACAT CAGGGAGTCA AGGGCACAAG AAAGGGGCAA GGACACCCAA AGTGCCAAAG
    CAAGGTGGCA TGAACTGGGC AGACCTACTG CCCCCTCCCC CAGCACATCC TCCTCCTCAT
    AGCAACAGTG AAGAGTACAG CATCTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGC
    CCCGTGCCCC CAGCACGGAT GTATCTGCAA CAAGGTGAAC TAGAAGAGGA GGAAGATGAA
    CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC GCCTCTTCTC CGGCTGCCGT GTCTTACAGC
    CATCAATCCA CCGCCACTCT CACGCCCTCT CCACAGGAAG AACTCCAGCC CATGTTACAG
    GATTGTCCCG AGGAGGTGGG CCATGGGCAG CACCAGCCTG ACAGGAGACG GCAGCCGGTG
    AGCCCACCTC CACCTCCAAG GCCGATTTCC CCACCCCACA CCTACGGCTA CATTTCAGGA
    CCCCTTGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG
    GAAGTAGCCA AGATGCAGAC CAGGAGGCTT TTGTTACGTG GGCTGGAGCA GACCCCTGCC
    TCCAGTGTCG GGGACCTGGA GAGCTCTGTC ACCGGGTCCA TGATCAATGG TTGGGGCTCT
    GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGCG TTAGTTCTTC TGATGGCTCT
    TTTTTCACTG ATGCCGATTT TGCCCAGGCG GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG
    AAAGTGGCCC GGCGCCAAAT GCAGGATGCT GCTGGCCGCC GCCATTTTCA TGCATCCCAG
    TGCCCTAGAC CCACAAGTCC TGTTTCTACA GACAGCAACG TGAGTGCTGC TGTAGTACAG
    AAGGCCAGAC CAGCCAAGAA ACAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC
    ACAGATGATC TTCCACCTCC CCCTGTGCCA CCACCTGCTA TCAAGTCACC CACTGCCCAG
    TCCAAGGCCC AGCTGGAGGT TCGACCCGTG ATGGTGCCAA AACTCCCTTC TATAGAAGCA
    AGAACAGACA GATCATCAGA TAGGAAAGGA GGCAATTACA AGGGGAGAGA AGTATTGGAT
    GGAAGACAAG TTGCTGATAT GCGACCAAAT CCAGGTGATG CCAGAGAAGC ACAGGAACAG
    CCAAATGACG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG
    GCTCATCTCA TCCAAGAGGA TATTCTGCCT TACTGTAGAC CTACTTTTCC GACATCAAAT
    AATCCCAGAG ATCCTAGTTC TTCAAGTTCT ATGTCATCAA GAGGATCAGG AAGCAGACAA
    AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAGA TGCAAGTTCT TGGAGGATAT
    GAAAGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201225.1
    CDS1138..5982
    Translation

    Target ORF information:

    RefSeq Version XM_005201168.3
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201168.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT
    GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG
    GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGC TGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACCCCTTA TGCCACCACC
    CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGTGGAGA CTCTGGCGAG
    AAGCACTGGA AGCCACCCGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGTGCAAATG ACACAATTGC TCCAACTATC
    CCTTACAACC AGTCATATGA CCAGAATACT GGAGGATCCT ACAACAGTTC AGACCGGGGA
    AGTAGTACAT CAGGGAGTCA AGGGCACAAG AAAGGGGCAA GGACACCCAA AGTGCCAAAG
    CAAGGTGGCA TGAACTGGGC AGACCTACTG CCCCCTCCCC CAGCACATCC TCCTCCTCAT
    AGCAACAGTG AAGAGTACAG CATCTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGC
    CCCGTGCCCC CAGCACGGAT GTATCTGCAA CAAGGTGAAC TAGAAGAGGA GGAAGATGAA
    CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC GCCTCTTCTC CGGCTGCCGT GTCTTACAGC
    CATCAATCCA CCGCCACTCT CACGCCCTCT CCACAGGAAG AACTCCAGCC CATGTTACAG
    GATTGTCCCG AGGAGGTGGG CCATGGGCAG CACCAGCCTG ACAGGAGACG GCAGCCGGTG
    AGCCCACCTC CACCTCCAAG GCCGATTTCC CCACCCCACA CCTACGGCTA CATTTCAGGA
    CCCCTTGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG
    GAAGTAGCCA AGATGCAGAC CAGGAGGCTT TTGTTACGTG GGCTGGAGCA GACCCCTGCC
    TCCAGTGTCG GGGACCTGGA GAGCTCTGTC ACCGGGTCCA TGATCAATGG TTGGGGCTCT
    GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGCG TTAGTTCTTC TGATGGCTCT
    TTTTTCACTG ATGCCGATTT TGCCCAGGCG GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG
    AAAGTGGCCC GGCGCCAAAT GCAGGATGCT GCTGGCCGCC GCCATTTTCA TGCATCCCAG
    TGCCCTAGAC CCACAAGTCC TGTTTCTACA GACAGCAACG TGAGTGCTGC TGTAGTACAG
    AAGGCCAGAC CAGCCAAGAA ACAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC
    ACAGATGATC TTCCACCTCC CCCTGTGCCA CCACCTGCTA TCAAGTCACC CACTGCCCAG
    TCCAAGGCCC AGCTGGAGGT TCGACCCGTG ATGGTGCCAA AACTCCCTTC TATAGAAGCA
    AGAACAGACA GATCATCAGA TAGGAAAGGA GGCAATTACA AGGGGAGAGA AGTATTGGAT
    GGAAGACAAG TTGCTGATAT GCGACCAAAT CCAGGTGATG CCAGAGAAGC ACAGGAACAG
    CCAAATGACG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG
    GCTCATCTCA TCCAAGAGGA TATTCTGCCT TACTGTAGAC CTACTTTTCC GACATCAAAT
    AATCCCAGAG ATCCTAGTTC TTCAAGTTCT ATGTCATCAA GAGGATCAGG AAGCAGACAA
    AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAGA TGCAAGTTCT TGGAGGATAT
    GAAAGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15495
    Clone ID Related Accession (Same CDS sequence) XM_005201168.3 , XM_005201168.2
    Accession Version XM_005201168.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005201168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT
    GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG
    GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGC TGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACCCCTTA TGCCACCACC
    CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGTGGAGA CTCTGGCGAG
    AAGCACTGGA AGCCACCCGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGTGCAAATG ACACAATTGC TCCAACTATC
    CCTTACAACC AGTCATATGA CCAGAATACT GGAGGATCCT ACAACAGTTC AGACCGGGGA
    AGTAGTACAT CAGGGAGTCA AGGGCACAAG AAAGGGGCAA GGACACCCAA AGTGCCAAAG
    CAAGGTGGCA TGAACTGGGC AGACCTACTG CCCCCTCCCC CAGCACATCC TCCTCCTCAT
    AGCAACAGTG AAGAGTACAG CATCTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGC
    CCCGTGCCCC CAGCACGGAT GTATCTGCAA CAAGGTGAAC TAGAAGAGGA GGAAGATGAA
    CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC GCCTCTTCTC CGGCTGCCGT GTCTTACAGC
    CATCAATCCA CCGCCACTCT CACGCCCTCT CCACAGGAAG AACTCCAGCC CATGTTACAG
    GATTGTCCCG AGGAGGTGGG CCATGGGCAG CACCAGCCTG ACAGGAGACG GCAGCCGGTG
    AGCCCACCTC CACCTCCAAG GCCGATTTCC CCACCCCACA CCTACGGCTA CATTTCAGGA
    CCCCTTGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG
    GAAGTAGCCA AGATGCAGAC CAGGAGGCTT TTGTTACGTG GGCTGGAGCA GACCCCTGCC
    TCCAGTGTCG GGGACCTGGA GAGCTCTGTC ACCGGGTCCA TGATCAATGG TTGGGGCTCT
    GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGCG TTAGTTCTTC TGATGGCTCT
    TTTTTCACTG ATGCCGATTT TGCCCAGGCG GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG
    AAAGTGGCCC GGCGCCAAAT GCAGGATGCT GCTGGCCGCC GCCATTTTCA TGCATCCCAG
    TGCCCTAGAC CCACAAGTCC TGTTTCTACA GACAGCAACG TGAGTGCTGC TGTAGTACAG
    AAGGCCAGAC CAGCCAAGAA ACAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC
    ACAGATGATC TTCCACCTCC CCCTGTGCCA CCACCTGCTA TCAAGTCACC CACTGCCCAG
    TCCAAGGCCC AGCTGGAGGT TCGACCCGTG ATGGTGCCAA AACTCCCTTC TATAGAAGCA
    AGAACAGACA GATCATCAGA TAGGAAAGGA GGCAATTACA AGGGGAGAGA AGTATTGGAT
    GGAAGACAAG TTGCTGATAT GCGACCAAAT CCAGGTGATG CCAGAGAAGC ACAGGAACAG
    CCAAATGACG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG
    GCTCATCTCA TCCAAGAGGA TATTCTGCCT TACTGTAGAC CTACTTTTCC GACATCAAAT
    AATCCCAGAG ATCCTAGTTC TTCAAGTTCT ATGTCATCAA GAGGATCAGG AAGCAGACAA
    AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAGA TGCAAGTTCT TGGAGGATAT
    GAAAGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201225.1
    CDS1014..5858
    Translation

    Target ORF information:

    RefSeq Version XM_005201168.2
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201168.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGAGG CGAAGCTGTG
    AGCAGTGGAG GGAGGCCAGG ACTTCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTT
    GCAGACACAT GGCCTAACAC TGGCAACAAT CACAATGACT GCTCCATCAA TTGCTGCACG
    GCAGGCAATG GGAACAGTGA CAGCAACTTG ACCACGTATA GTCGCCCAGC TGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ATGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACCCCTTA TGCCACCACC
    CAGCTCATCC AGTCAAACCT CAGCAACAAC ATGAACAATG GCAGTGGAGA CTCTGGCGAG
    AAGCACTGGA AGCCACCCGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGTGCAAATG ACACAATTGC TCCAACTATC
    CCTTACAACC AGTCATATGA CCAGAATACT GGAGGATCCT ACAACAGTTC AGACCGGGGA
    AGTAGTACAT CAGGGAGTCA AGGGCACAAG AAAGGGGCAA GGACACCCAA AGTGCCAAAG
    CAAGGTGGCA TGAACTGGGC AGACCTACTG CCCCCTCCCC CAGCACATCC TCCTCCTCAT
    AGCAACAGTG AAGAGTACAG CATCTCTGTA GATGAAAGCT ATGACCAAGA AATGCCATGC
    CCCGTGCCCC CAGCACGGAT GTATCTGCAA CAAGGTGAAC TAGAAGAGGA GGAAGATGAA
    CGAGGTCCCA CTCCCCCTGT GCGGGGAGCC GCCTCTTCTC CGGCTGCCGT GTCTTACAGC
    CATCAATCCA CCGCCACTCT CACGCCCTCT CCACAGGAAG AACTCCAGCC CATGTTACAG
    GATTGTCCCG AGGAGGTGGG CCATGGGCAG CACCAGCCTG ACAGGAGACG GCAGCCGGTG
    AGCCCACCTC CACCTCCAAG GCCGATTTCC CCACCCCACA CCTACGGCTA CATTTCAGGA
    CCCCTTGTCT CAGATATGGA TACGGATGCG CCAGAAGAGG AAGAAGACGA AGCCGACATG
    GAAGTAGCCA AGATGCAGAC CAGGAGGCTT TTGTTACGTG GGCTGGAGCA GACCCCTGCC
    TCCAGTGTCG GGGACCTGGA GAGCTCTGTC ACCGGGTCCA TGATCAATGG TTGGGGCTCT
    GCCTCAGAGG AGGACAACAT TTCCAGCGGA CGCTCCAGCG TTAGTTCTTC TGATGGCTCT
    TTTTTCACTG ATGCCGATTT TGCCCAGGCG GTCGCAGCAG CAGCGGAGTA TGCTGGTCTG
    AAAGTGGCCC GGCGCCAAAT GCAGGATGCT GCTGGCCGCC GCCATTTTCA TGCATCCCAG
    TGCCCTAGAC CCACAAGTCC TGTTTCTACA GACAGCAACG TGAGTGCTGC TGTAGTACAG
    AAGGCCAGAC CAGCCAAGAA ACAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC
    ACAGATGATC TTCCACCTCC CCCTGTGCCA CCACCTGCTA TCAAGTCACC CACTGCCCAG
    TCCAAGGCCC AGCTGGAGGT TCGACCCGTG ATGGTGCCAA AACTCCCTTC TATAGAAGCA
    AGAACAGACA GATCATCAGA TAGGAAAGGA GGCAATTACA AGGGGAGAGA AGTATTGGAT
    GGAAGACAAG TTGCTGATAT GCGACCAAAT CCAGGTGATG CCAGAGAAGC ACAGGAACAG
    CCAAATGACG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GAGACCTTCC ACCAGCAAAG
    GCTCATCTCA TCCAAGAGGA TATTCTGCCT TACTGTAGAC CTACTTTTCC GACATCAAAT
    AATCCCAGAG ATCCTAGTTC TTCAAGTTCT ATGTCATCAA GAGGATCAGG AAGCAGACAA
    AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAGA TGCAAGTTCT TGGAGGATAT
    GAAAGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15496
    Clone ID Related Accession (Same CDS sequence) XM_005201169.3
    Accession Version XM_005201169.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC
    CAGGTGGTTG CCTTGGGACG AACGGTCACT TTCCAGTGTG AAGCAACAGG AAATCCCCAA
    CCAGCTATCT TCTGGAGGAG AGAGGGGAGT CAGAATCTGC TTTTCTCATA TCAGCCACCA
    CAGTCATCCA GCCGTTTTTC AGTCTCCCAG ACTGGTGACC TCACTATTAC TAATGTCCAG
    CGATCTGATG TTGGTTATTA TATCTGCCAG ACTTTGAATG TTGCCGGAAG TATCATCACA
    AAAGCATATT TGGAAGTTAC CGATGTGATT GCAGACCGAC CTCCCCCAGT CATTCGACAA
    GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGTACTTTAG TGCTCAGCTG TGTGGCCACA
    GGCAGTCCAG TGCCCACAAT TCTGTGGAGA AAGGATGGAG TCCTTGTTTC AACCCAAGAT
    TCTCGAATCA AACAGCTGGA GACCGGAGTA CTACAGATCC GATATGCTAA GCTGGGTGAT
    ACTGGTCGAT ACACGTGCAT TGCATCAACC CCCAGTGGTG AAGCAACGTG GAGTGCTTAC
    ATTGAAGTCC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCCACCGA CCCAAATTTA
    ATCCCTAGTG CCCCCTCAAA ACCTGAAGTT ACAGATGTCA GCCGAAATAC AGTAACGTTG
    TCATGGCAAC CAAATTTGAA TTCAGGTGCA ACTCCGACGT CTTATATTAT AGAAGCCTTC
    AGCCATGCAT CTGGTAGCAG CTGGCAGACT GTAGCGGAGA ATGTGAAAAC AGAAACATTT
    GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCCAATGCA
    TATGGAATTA GTGATCCAAG CCAGATATCA GACCCAGTGA AAACACAAGA TGTTCCCCCA
    ACCAGTCAGG GGGTGGATCA CAAGCAGGTC CAGCGAGAAC TGGGAAATGT TGTACTGCAT
    CTCCACAACC CCACCATCCT TTCCTCCTCT TCAATCGAAG TGCACTGGAC AGTGGACCAA
    CAGTCTCAGT ATATTCAAGG ATACAAAGTT CTTTACCGCC CGTCTGGAGC CAACCATGGT
    GAATCCGAGT GGTTAGTTTT TGAAGTGAGG ACCCCAACCA AAAACAGTGT GGTTATCCCT
    GATCTCAAGA AAGGAGTCAA CTATGAAATT AAGGCTCGTC CTTTTTTTAA TGAATTTCAA
    GGAGCAGATA GTGAAATTAA ATTTGCCAAA ACCCTAGAAG AAGCACCCAG TGCCCCACCT
    CAAAGTGTGA CTGTATCAAA GAATGATGGA AATGGAACTG CAATTCTTGT TAGTTGGCAG
    CCACCTCCTG AAGATACTCA AAATGGAATG GTCCAAGAGT ATAAGGTCTG GTGTCTGGGC
    AATGAAACGC GATACCATAT AAACAAGACA GTAGATGGTT CCACCTTTTC TGTGGTTATT
    CCCTCACTGG TTCCTGGGAT CCGATATAGT GTAGAGGTGG CAGCCAGCAC GGGGGCTGGG
    TCGGGGGTGA AGAGTGAGCC ACAATTCATC CAGTTAGATT CTCATGGAAA CCCCGTGTCA
    CCTGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCAGACG TAGTGAAGCA GCCGGCATTC
    ATCGCAGGAA TCGGGGCTGC CTGTTGGATC ATCCTTATGG TTTTTAGCAT CTGGCTCTAT
    CGACACCGAA AGAAGAGAAA TGGGCTGGCT AGTACTTATG CGGGTATCAG AAAAGTCCCG
    TCTTTTACCT TCACACCAAC AGTAACTTAT CAGAGAGGAG GCGAAGCTGT GAGCAGTGGA
    GGGAGGCCAG GACTTCTCAA CATCAGTGAA CCTGCCACAC AGCCATGGCT TGCAGACACA
    TGGCCTAACA CTGGCAACAA TCACAATGAC TGCTCCATCA ATTGCTGCAC GGCAGGCAAT
    GGGAACAGTG ACAGCAACTT GACCACGTAT AGTCGCCCAG CTGATTGTAT AGCAAATTAT
    AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT
    GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG
    GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACCCCTT ATGCCACCAC CCAGCTCATC
    CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGTGGAG ACTCTGGCGA GAAGCACTGG
    AAGCCACCCG GACAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAACTGA ACAAAGATTA TCGTGCAAAT GACACAATTG CTCCAACTAT CCCTTACAAC
    CAGTCATATG ACCAGAATAC TGGAGGATCC TACAACAGTT CAGACCGGGG AAGTAGTACA
    TCAGGGAGTC AAGGGCACAA GAAAGGGGCA AGGACACCCA AAGTGCCAAA GCAAGGTGGC
    ATGAACTGGG CAGACCTACT GCCCCCTCCC CCAGCACATC CTCCTCCTCA TAGCAACAGT
    GAAGAGTACA GCATCTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG CCCCGTGCCC
    CCAGCACGGA TGTATCTGCA ACAAGGTGAA CTAGAAGAGG AGGAAGATGA ACGAGGTCCC
    ACTCCCCCTG TGCGGGGAGC CGCCTCTTCT CCGGCTGCCG TGTCTTACAG CCATCAATCC
    ACCGCCACTC TCACGCCCTC TCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCC
    GAGGAGGTGG GCCATGGGCA GCACCAGCCT GACAGGAGAC GGCAGCCGGT GAGCCCACCT
    CCACCTCCAA GGCCGATTTC CCCACCCCAC ACCTACGGCT ACATTTCAGG ACCCCTTGTC
    TCAGATATGG ATACGGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAAGTAGCC
    AAGATGCAGA CCAGGAGGCT TTTGTTACGT GGGCTGGAGC AGACCCCTGC CTCCAGTGTC
    GGGGACCTGG AGAGCTCTGT CACCGGGTCC ATGATCAATG GTTGGGGCTC TGCCTCAGAG
    GAGGACAACA TTTCCAGCGG ACGCTCCAGC GTTAGTTCTT CTGATGGCTC TTTTTTCACT
    GATGCCGATT TTGCCCAGGC GGTCGCAGCA GCAGCGGAGT ATGCTGGTCT GAAAGTGGCC
    CGGCGCCAAA TGCAGGATGC TGCTGGCCGC CGCCATTTTC ATGCATCCCA GTGCCCTAGA
    CCCACAAGTC CTGTTTCTAC AGACAGCAAC GTGAGTGCTG CTGTAGTACA GAAGGCCAGA
    CCAGCCAAGA AACAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGCCTA CACAGATGAT
    CTTCCACCTC CCCCTGTGCC ACCACCTGCT ATCAAGTCAC CCACTGCCCA GTCCAAGGCC
    CAGCTGGAGG TTCGACCCGT GATGGTGCCA AAACTCCCTT CTATAGAAGC AAGAACAGAC
    AGATCATCAG ATAGGAAAGG AGGCAATTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAA
    GTTGCTGATA TGCGACCAAA TCCAGGTGAT GCCAGAGAAG CACAGGAACA GCCAAATGAC
    GGGAAAGGAC GAGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GGCTCATCTC
    ATCCAAGAGG ATATTCTGCC TTACTGTAGA CCTACTTTTC CGACATCAAA TAATCCCAGA
    GATCCTAGTT CTTCAAGTTC TATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA
    GCAAATGTAG GTCGAAGAAA TATTGCAGAG ATGCAAGTTC TTGGAGGATA TGAAAGAGAA
    GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201226.1
    CDS1..4836
    Translation

    Target ORF information:

    RefSeq Version XM_005201169.3
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201169.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC
    CAGGTGGTTG CCTTGGGACG AACGGTCACT TTCCAGTGTG AAGCAACAGG AAATCCCCAA
    CCAGCTATCT TCTGGAGGAG AGAGGGGAGT CAGAATCTGC TTTTCTCATA TCAGCCACCA
    CAGTCATCCA GCCGTTTTTC AGTCTCCCAG ACTGGTGACC TCACTATTAC TAATGTCCAG
    CGATCTGATG TTGGTTATTA TATCTGCCAG ACTTTGAATG TTGCCGGAAG TATCATCACA
    AAAGCATATT TGGAAGTTAC CGATGTGATT GCAGACCGAC CTCCCCCAGT CATTCGACAA
    GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGTACTTTAG TGCTCAGCTG TGTGGCCACA
    GGCAGTCCAG TGCCCACAAT TCTGTGGAGA AAGGATGGAG TCCTTGTTTC AACCCAAGAT
    TCTCGAATCA AACAGCTGGA GACCGGAGTA CTACAGATCC GATATGCTAA GCTGGGTGAT
    ACTGGTCGAT ACACGTGCAT TGCATCAACC CCCAGTGGTG AAGCAACGTG GAGTGCTTAC
    ATTGAAGTCC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCCACCGA CCCAAATTTA
    ATCCCTAGTG CCCCCTCAAA ACCTGAAGTT ACAGATGTCA GCCGAAATAC AGTAACGTTG
    TCATGGCAAC CAAATTTGAA TTCAGGTGCA ACTCCGACGT CTTATATTAT AGAAGCCTTC
    AGCCATGCAT CTGGTAGCAG CTGGCAGACT GTAGCGGAGA ATGTGAAAAC AGAAACATTT
    GCCATTAAAG GACTCAAACC TAATGCAATT TACCTTTTCC TTGTGAGGGC AGCCAATGCA
    TATGGAATTA GTGATCCAAG CCAGATATCA GACCCAGTGA AAACACAAGA TGTTCCCCCA
    ACCAGTCAGG GGGTGGATCA CAAGCAGGTC CAGCGAGAAC TGGGAAATGT TGTACTGCAT
    CTCCACAACC CCACCATCCT TTCCTCCTCT TCAATCGAAG TGCACTGGAC AGTGGACCAA
    CAGTCTCAGT ATATTCAAGG ATACAAAGTT CTTTACCGCC CGTCTGGAGC CAACCATGGT
    GAATCCGAGT GGTTAGTTTT TGAAGTGAGG ACCCCAACCA AAAACAGTGT GGTTATCCCT
    GATCTCAAGA AAGGAGTCAA CTATGAAATT AAGGCTCGTC CTTTTTTTAA TGAATTTCAA
    GGAGCAGATA GTGAAATTAA ATTTGCCAAA ACCCTAGAAG AAGCACCCAG TGCCCCACCT
    CAAAGTGTGA CTGTATCAAA GAATGATGGA AATGGAACTG CAATTCTTGT TAGTTGGCAG
    CCACCTCCTG AAGATACTCA AAATGGAATG GTCCAAGAGT ATAAGGTCTG GTGTCTGGGC
    AATGAAACGC GATACCATAT AAACAAGACA GTAGATGGTT CCACCTTTTC TGTGGTTATT
    CCCTCACTGG TTCCTGGGAT CCGATATAGT GTAGAGGTGG CAGCCAGCAC GGGGGCTGGG
    TCGGGGGTGA AGAGTGAGCC ACAATTCATC CAGTTAGATT CTCATGGAAA CCCCGTGTCA
    CCTGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCAGACG TAGTGAAGCA GCCGGCATTC
    ATCGCAGGAA TCGGGGCTGC CTGTTGGATC ATCCTTATGG TTTTTAGCAT CTGGCTCTAT
    CGACACCGAA AGAAGAGAAA TGGGCTGGCT AGTACTTATG CGGGTATCAG AAAAGTCCCG
    TCTTTTACCT TCACACCAAC AGTAACTTAT CAGAGAGGAG GCGAAGCTGT GAGCAGTGGA
    GGGAGGCCAG GACTTCTCAA CATCAGTGAA CCTGCCACAC AGCCATGGCT TGCAGACACA
    TGGCCTAACA CTGGCAACAA TCACAATGAC TGCTCCATCA ATTGCTGCAC GGCAGGCAAT
    GGGAACAGTG ACAGCAACTT GACCACGTAT AGTCGCCCAG CTGATTGTAT AGCAAATTAT
    AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT
    GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG
    GATGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACCCCTT ATGCCACCAC CCAGCTCATC
    CAGTCAAACC TCAGCAACAA CATGAACAAT GGCAGTGGAG ACTCTGGCGA GAAGCACTGG
    AAGCCACCCG GACAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAACTGA ACAAAGATTA TCGTGCAAAT GACACAATTG CTCCAACTAT CCCTTACAAC
    CAGTCATATG ACCAGAATAC TGGAGGATCC TACAACAGTT CAGACCGGGG AAGTAGTACA
    TCAGGGAGTC AAGGGCACAA GAAAGGGGCA AGGACACCCA AAGTGCCAAA GCAAGGTGGC
    ATGAACTGGG CAGACCTACT GCCCCCTCCC CCAGCACATC CTCCTCCTCA TAGCAACAGT
    GAAGAGTACA GCATCTCTGT AGATGAAAGC TATGACCAAG AAATGCCATG CCCCGTGCCC
    CCAGCACGGA TGTATCTGCA ACAAGGTGAA CTAGAAGAGG AGGAAGATGA ACGAGGTCCC
    ACTCCCCCTG TGCGGGGAGC CGCCTCTTCT CCGGCTGCCG TGTCTTACAG CCATCAATCC
    ACCGCCACTC TCACGCCCTC TCCACAGGAA GAACTCCAGC CCATGTTACA GGATTGTCCC
    GAGGAGGTGG GCCATGGGCA GCACCAGCCT GACAGGAGAC GGCAGCCGGT GAGCCCACCT
    CCACCTCCAA GGCCGATTTC CCCACCCCAC ACCTACGGCT ACATTTCAGG ACCCCTTGTC
    TCAGATATGG ATACGGATGC GCCAGAAGAG GAAGAAGACG AAGCCGACAT GGAAGTAGCC
    AAGATGCAGA CCAGGAGGCT TTTGTTACGT GGGCTGGAGC AGACCCCTGC CTCCAGTGTC
    GGGGACCTGG AGAGCTCTGT CACCGGGTCC ATGATCAATG GTTGGGGCTC TGCCTCAGAG
    GAGGACAACA TTTCCAGCGG ACGCTCCAGC GTTAGTTCTT CTGATGGCTC TTTTTTCACT
    GATGCCGATT TTGCCCAGGC GGTCGCAGCA GCAGCGGAGT ATGCTGGTCT GAAAGTGGCC
    CGGCGCCAAA TGCAGGATGC TGCTGGCCGC CGCCATTTTC ATGCATCCCA GTGCCCTAGA
    CCCACAAGTC CTGTTTCTAC AGACAGCAAC GTGAGTGCTG CTGTAGTACA GAAGGCCAGA
    CCAGCCAAGA AACAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGCCTA CACAGATGAT
    CTTCCACCTC CCCCTGTGCC ACCACCTGCT ATCAAGTCAC CCACTGCCCA GTCCAAGGCC
    CAGCTGGAGG TTCGACCCGT GATGGTGCCA AAACTCCCTT CTATAGAAGC AAGAACAGAC
    AGATCATCAG ATAGGAAAGG AGGCAATTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAA
    GTTGCTGATA TGCGACCAAA TCCAGGTGAT GCCAGAGAAG CACAGGAACA GCCAAATGAC
    GGGAAAGGAC GAGGAAACAA GGCAGCAAAA CGAGACCTTC CACCAGCAAA GGCTCATCTC
    ATCCAAGAGG ATATTCTGCC TTACTGTAGA CCTACTTTTC CGACATCAAA TAATCCCAGA
    GATCCTAGTT CTTCAAGTTC TATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA
    GCAAATGTAG GTCGAAGAAA TATTGCAGAG ATGCAAGTTC TTGGAGGATA TGAAAGAGAA
    GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15497
    Clone ID Related Accession (Same CDS sequence) XM_005201170.4 , XM_005201170.3
    Accession Version XM_005201170.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201170.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC
    AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC
    TTGACCACGT ATAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACCCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGTGG AGACTCTGGC GAGAAGCACT GGAAGCCACC CGGACAGCAG
    AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGTGCAA ATGACACAAT TGCTCCAACT ATCCCTTACA ACCAGTCATA TGACCAGAAT
    ACTGGAGGAT CCTACAACAG TTCAGACCGG GGAAGTAGTA CATCAGGGAG TCAAGGGCAC
    AAGAAAGGGG CAAGGACACC CAAAGTGCCA AAGCAAGGTG GCATGAACTG GGCAGACCTA
    CTGCCCCCTC CCCCAGCACA TCCTCCTCCT CATAGCAACA GTGAAGAGTA CAGCATCTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGCCCCGTGC CCCCAGCACG GATGTATCTG
    CAACAAGGTG AACTAGAAGA GGAGGAAGAT GAACGAGGTC CCACTCCCCC TGTGCGGGGA
    GCCGCCTCTT CTCCGGCTGC CGTGTCTTAC AGCCATCAAT CCACCGCCAC TCTCACGCCC
    TCTCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CCGAGGAGGT GGGCCATGGG
    CAGCACCAGC CTGACAGGAG ACGGCAGCCG GTGAGCCCAC CTCCACCTCC AAGGCCGATT
    TCCCCACCCC ACACCTACGG CTACATTTCA GGACCCCTTG TCTCAGATAT GGATACGGAT
    GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAAGTAG CCAAGATGCA GACCAGGAGG
    CTTTTGTTAC GTGGGCTGGA GCAGACCCCT GCCTCCAGTG TCGGGGACCT GGAGAGCTCT
    GTCACCGGGT CCATGATCAA TGGTTGGGGC TCTGCCTCAG AGGAGGACAA CATTTCCAGC
    GGACGCTCCA GCGTTAGTTC TTCTGATGGC TCTTTTTTCA CTGATGCCGA TTTTGCCCAG
    GCGGTCGCAG CAGCAGCGGA GTATGCTGGT CTGAAAGTGG CCCGGCGCCA AATGCAGGAT
    GCTGCTGGCC GCCGCCATTT TCATGCATCC CAGTGCCCTA GACCCACAAG TCCTGTTTCT
    ACAGACAGCA ACGTGAGTGC TGCTGTAGTA CAGAAGGCCA GACCAGCCAA GAAACAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCCCCTGTG
    CCACCACCTG CTATCAAGTC ACCCACTGCC CAGTCCAAGG CCCAGCTGGA GGTTCGACCC
    GTGATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACAGATCATC AGATAGGAAA
    GGAGGCAATT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCTGA TATGCGACCA
    AATCCAGGTG ATGCCAGAGA AGCACAGGAA CAGCCAAATG ACGGGAAAGG ACGAGGAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGGCTCATC TCATCCAAGA GGATATTCTG
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGT
    TCTATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATTGCAG AGATGCAAGT TCTTGGAGGA TATGAAAGAG AAGATAATAA TGAAGAATTA
    GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201227.1
    CDS1113..5930
    Translation

    Target ORF information:

    RefSeq Version XM_005201170.4
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201170.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC
    AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC
    TTGACCACGT ATAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACCCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGTGG AGACTCTGGC GAGAAGCACT GGAAGCCACC CGGACAGCAG
    AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGTGCAA ATGACACAAT TGCTCCAACT ATCCCTTACA ACCAGTCATA TGACCAGAAT
    ACTGGAGGAT CCTACAACAG TTCAGACCGG GGAAGTAGTA CATCAGGGAG TCAAGGGCAC
    AAGAAAGGGG CAAGGACACC CAAAGTGCCA AAGCAAGGTG GCATGAACTG GGCAGACCTA
    CTGCCCCCTC CCCCAGCACA TCCTCCTCCT CATAGCAACA GTGAAGAGTA CAGCATCTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGCCCCGTGC CCCCAGCACG GATGTATCTG
    CAACAAGGTG AACTAGAAGA GGAGGAAGAT GAACGAGGTC CCACTCCCCC TGTGCGGGGA
    GCCGCCTCTT CTCCGGCTGC CGTGTCTTAC AGCCATCAAT CCACCGCCAC TCTCACGCCC
    TCTCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CCGAGGAGGT GGGCCATGGG
    CAGCACCAGC CTGACAGGAG ACGGCAGCCG GTGAGCCCAC CTCCACCTCC AAGGCCGATT
    TCCCCACCCC ACACCTACGG CTACATTTCA GGACCCCTTG TCTCAGATAT GGATACGGAT
    GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAAGTAG CCAAGATGCA GACCAGGAGG
    CTTTTGTTAC GTGGGCTGGA GCAGACCCCT GCCTCCAGTG TCGGGGACCT GGAGAGCTCT
    GTCACCGGGT CCATGATCAA TGGTTGGGGC TCTGCCTCAG AGGAGGACAA CATTTCCAGC
    GGACGCTCCA GCGTTAGTTC TTCTGATGGC TCTTTTTTCA CTGATGCCGA TTTTGCCCAG
    GCGGTCGCAG CAGCAGCGGA GTATGCTGGT CTGAAAGTGG CCCGGCGCCA AATGCAGGAT
    GCTGCTGGCC GCCGCCATTT TCATGCATCC CAGTGCCCTA GACCCACAAG TCCTGTTTCT
    ACAGACAGCA ACGTGAGTGC TGCTGTAGTA CAGAAGGCCA GACCAGCCAA GAAACAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCCCCTGTG
    CCACCACCTG CTATCAAGTC ACCCACTGCC CAGTCCAAGG CCCAGCTGGA GGTTCGACCC
    GTGATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACAGATCATC AGATAGGAAA
    GGAGGCAATT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCTGA TATGCGACCA
    AATCCAGGTG ATGCCAGAGA AGCACAGGAA CAGCCAAATG ACGGGAAAGG ACGAGGAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGGCTCATC TCATCCAAGA GGATATTCTG
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGT
    TCTATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATTGCAG AGATGCAAGT TCTTGGAGGA TATGAAAGAG AAGATAATAA TGAAGAATTA
    GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15497
    Clone ID Related Accession (Same CDS sequence) XM_005201170.4 , XM_005201170.3
    Accession Version XM_005201170.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC
    AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC
    TTGACCACGT ATAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACCCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGTGG AGACTCTGGC GAGAAGCACT GGAAGCCACC CGGACAGCAG
    AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGTGCAA ATGACACAAT TGCTCCAACT ATCCCTTACA ACCAGTCATA TGACCAGAAT
    ACTGGAGGAT CCTACAACAG TTCAGACCGG GGAAGTAGTA CATCAGGGAG TCAAGGGCAC
    AAGAAAGGGG CAAGGACACC CAAAGTGCCA AAGCAAGGTG GCATGAACTG GGCAGACCTA
    CTGCCCCCTC CCCCAGCACA TCCTCCTCCT CATAGCAACA GTGAAGAGTA CAGCATCTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGCCCCGTGC CCCCAGCACG GATGTATCTG
    CAACAAGGTG AACTAGAAGA GGAGGAAGAT GAACGAGGTC CCACTCCCCC TGTGCGGGGA
    GCCGCCTCTT CTCCGGCTGC CGTGTCTTAC AGCCATCAAT CCACCGCCAC TCTCACGCCC
    TCTCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CCGAGGAGGT GGGCCATGGG
    CAGCACCAGC CTGACAGGAG ACGGCAGCCG GTGAGCCCAC CTCCACCTCC AAGGCCGATT
    TCCCCACCCC ACACCTACGG CTACATTTCA GGACCCCTTG TCTCAGATAT GGATACGGAT
    GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAAGTAG CCAAGATGCA GACCAGGAGG
    CTTTTGTTAC GTGGGCTGGA GCAGACCCCT GCCTCCAGTG TCGGGGACCT GGAGAGCTCT
    GTCACCGGGT CCATGATCAA TGGTTGGGGC TCTGCCTCAG AGGAGGACAA CATTTCCAGC
    GGACGCTCCA GCGTTAGTTC TTCTGATGGC TCTTTTTTCA CTGATGCCGA TTTTGCCCAG
    GCGGTCGCAG CAGCAGCGGA GTATGCTGGT CTGAAAGTGG CCCGGCGCCA AATGCAGGAT
    GCTGCTGGCC GCCGCCATTT TCATGCATCC CAGTGCCCTA GACCCACAAG TCCTGTTTCT
    ACAGACAGCA ACGTGAGTGC TGCTGTAGTA CAGAAGGCCA GACCAGCCAA GAAACAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCCCCTGTG
    CCACCACCTG CTATCAAGTC ACCCACTGCC CAGTCCAAGG CCCAGCTGGA GGTTCGACCC
    GTGATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACAGATCATC AGATAGGAAA
    GGAGGCAATT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCTGA TATGCGACCA
    AATCCAGGTG ATGCCAGAGA AGCACAGGAA CAGCCAAATG ACGGGAAAGG ACGAGGAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGGCTCATC TCATCCAAGA GGATATTCTG
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGT
    TCTATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATTGCAG AGATGCAAGT TCTTGGAGGA TATGAAAGAG AAGATAATAA TGAAGAATTA
    GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201227.1
    CDS1..4818
    Translation

    Target ORF information:

    RefSeq Version XM_005201170.3
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201170.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC
    AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC
    TTGACCACGT ATAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGATGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACCCC TTATGCCACC ACCCAGCTCA TCCAGTCAAA CCTCAGCAAC
    AACATGAACA ATGGCAGTGG AGACTCTGGC GAGAAGCACT GGAAGCCACC CGGACAGCAG
    AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT
    TATCGTGCAA ATGACACAAT TGCTCCAACT ATCCCTTACA ACCAGTCATA TGACCAGAAT
    ACTGGAGGAT CCTACAACAG TTCAGACCGG GGAAGTAGTA CATCAGGGAG TCAAGGGCAC
    AAGAAAGGGG CAAGGACACC CAAAGTGCCA AAGCAAGGTG GCATGAACTG GGCAGACCTA
    CTGCCCCCTC CCCCAGCACA TCCTCCTCCT CATAGCAACA GTGAAGAGTA CAGCATCTCT
    GTAGATGAAA GCTATGACCA AGAAATGCCA TGCCCCGTGC CCCCAGCACG GATGTATCTG
    CAACAAGGTG AACTAGAAGA GGAGGAAGAT GAACGAGGTC CCACTCCCCC TGTGCGGGGA
    GCCGCCTCTT CTCCGGCTGC CGTGTCTTAC AGCCATCAAT CCACCGCCAC TCTCACGCCC
    TCTCCACAGG AAGAACTCCA GCCCATGTTA CAGGATTGTC CCGAGGAGGT GGGCCATGGG
    CAGCACCAGC CTGACAGGAG ACGGCAGCCG GTGAGCCCAC CTCCACCTCC AAGGCCGATT
    TCCCCACCCC ACACCTACGG CTACATTTCA GGACCCCTTG TCTCAGATAT GGATACGGAT
    GCGCCAGAAG AGGAAGAAGA CGAAGCCGAC ATGGAAGTAG CCAAGATGCA GACCAGGAGG
    CTTTTGTTAC GTGGGCTGGA GCAGACCCCT GCCTCCAGTG TCGGGGACCT GGAGAGCTCT
    GTCACCGGGT CCATGATCAA TGGTTGGGGC TCTGCCTCAG AGGAGGACAA CATTTCCAGC
    GGACGCTCCA GCGTTAGTTC TTCTGATGGC TCTTTTTTCA CTGATGCCGA TTTTGCCCAG
    GCGGTCGCAG CAGCAGCGGA GTATGCTGGT CTGAAAGTGG CCCGGCGCCA AATGCAGGAT
    GCTGCTGGCC GCCGCCATTT TCATGCATCC CAGTGCCCTA GACCCACAAG TCCTGTTTCT
    ACAGACAGCA ACGTGAGTGC TGCTGTAGTA CAGAAGGCCA GACCAGCCAA GAAACAAAAA
    CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCCCCTGTG
    CCACCACCTG CTATCAAGTC ACCCACTGCC CAGTCCAAGG CCCAGCTGGA GGTTCGACCC
    GTGATGGTGC CAAAACTCCC TTCTATAGAA GCAAGAACAG ACAGATCATC AGATAGGAAA
    GGAGGCAATT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCTGA TATGCGACCA
    AATCCAGGTG ATGCCAGAGA AGCACAGGAA CAGCCAAATG ACGGGAAAGG ACGAGGAAAC
    AAGGCAGCAA AACGAGACCT TCCACCAGCA AAGGCTCATC TCATCCAAGA GGATATTCTG
    CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGT
    TCTATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA
    AATATTGCAG AGATGCAAGT TCTTGGAGGA TATGAAAGAG AAGATAATAA TGAAGAATTA
    GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15498
    Clone ID Related Accession (Same CDS sequence) XM_005201171.4 , XM_005201171.3
    Accession Version XM_005201171.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201171.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGGGCA GCCCACCCCT
    TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGTGGA
    GACTCTGGCG AGAAGCACTG GAAGCCACCC GGACAGCAGA AACAGGAAGT GGCACCAATT
    CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGTGCAAA TGACACAATT
    GCTCCAACTA TCCCTTACAA CCAGTCATAT GACCAGAATA CTGGAGGATC CTACAACAGT
    TCAGACCGGG GAAGTAGTAC ATCAGGGAGT CAAGGGCACA AGAAAGGGGC AAGGACACCC
    AAAGTGCCAA AGCAAGGTGG CATGAACTGG GCAGACCTAC TGCCCCCTCC CCCAGCACAT
    CCTCCTCCTC ATAGCAACAG TGAAGAGTAC AGCATCTCTG TAGATGAAAG CTATGACCAA
    GAAATGCCAT GCCCCGTGCC CCCAGCACGG ATGTATCTGC AACAAGGTGA ACTAGAAGAG
    GAGGAAGATG AACGAGGTCC CACTCCCCCT GTGCGGGGAG CCGCCTCTTC TCCGGCTGCC
    GTGTCTTACA GCCATCAATC CACCGCCACT CTCACGCCCT CTCCACAGGA AGAACTCCAG
    CCCATGTTAC AGGATTGTCC CGAGGAGGTG GGCCATGGGC AGCACCAGCC TGACAGGAGA
    CGGCAGCCGG TGAGCCCACC TCCACCTCCA AGGCCGATTT CCCCACCCCA CACCTACGGC
    TACATTTCAG GACCCCTTGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC
    GAAGCCGACA TGGAAGTAGC CAAGATGCAG ACCAGGAGGC TTTTGTTACG TGGGCTGGAG
    CAGACCCCTG CCTCCAGTGT CGGGGACCTG GAGAGCTCTG TCACCGGGTC CATGATCAAT
    GGTTGGGGCT CTGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG CGTTAGTTCT
    TCTGATGGCT CTTTTTTCAC TGATGCCGAT TTTGCCCAGG CGGTCGCAGC AGCAGCGGAG
    TATGCTGGTC TGAAAGTGGC CCGGCGCCAA ATGCAGGATG CTGCTGGCCG CCGCCATTTT
    CATGCATCCC AGTGCCCTAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CGTGAGTGCT
    GCTGTAGTAC AGAAGGCCAG ACCAGCCAAG AAACAAAAAC ACCAGCCAGG ACATCTGCGC
    AGAGAAGCCT ACACAGATGA TCTTCCACCT CCCCCTGTGC CACCACCTGC TATCAAGTCA
    CCCACTGCCC AGTCCAAGGC CCAGCTGGAG GTTCGACCCG TGATGGTGCC AAAACTCCCT
    TCTATAGAAG CAAGAACAGA CAGATCATCA GATAGGAAAG GAGGCAATTA CAAGGGGAGA
    GAAGTATTGG ATGGAAGACA AGTTGCTGAT ATGCGACCAA ATCCAGGTGA TGCCAGAGAA
    GCACAGGAAC AGCCAAATGA CGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGAGACCTT
    CCACCAGCAA AGGCTCATCT CATCCAAGAG GATATTCTGC CTTACTGTAG ACCTACTTTT
    CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGTT CTATGTCATC AAGAGGATCA
    GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA GATGCAAGTT
    CTTGGAGGAT ATGAAAGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201228.1
    CDS297..5093
    Translation

    Target ORF information:

    RefSeq Version XM_005201171.4
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201171.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGGGCA GCCCACCCCT
    TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGTGGA
    GACTCTGGCG AGAAGCACTG GAAGCCACCC GGACAGCAGA AACAGGAAGT GGCACCAATT
    CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGTGCAAA TGACACAATT
    GCTCCAACTA TCCCTTACAA CCAGTCATAT GACCAGAATA CTGGAGGATC CTACAACAGT
    TCAGACCGGG GAAGTAGTAC ATCAGGGAGT CAAGGGCACA AGAAAGGGGC AAGGACACCC
    AAAGTGCCAA AGCAAGGTGG CATGAACTGG GCAGACCTAC TGCCCCCTCC CCCAGCACAT
    CCTCCTCCTC ATAGCAACAG TGAAGAGTAC AGCATCTCTG TAGATGAAAG CTATGACCAA
    GAAATGCCAT GCCCCGTGCC CCCAGCACGG ATGTATCTGC AACAAGGTGA ACTAGAAGAG
    GAGGAAGATG AACGAGGTCC CACTCCCCCT GTGCGGGGAG CCGCCTCTTC TCCGGCTGCC
    GTGTCTTACA GCCATCAATC CACCGCCACT CTCACGCCCT CTCCACAGGA AGAACTCCAG
    CCCATGTTAC AGGATTGTCC CGAGGAGGTG GGCCATGGGC AGCACCAGCC TGACAGGAGA
    CGGCAGCCGG TGAGCCCACC TCCACCTCCA AGGCCGATTT CCCCACCCCA CACCTACGGC
    TACATTTCAG GACCCCTTGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC
    GAAGCCGACA TGGAAGTAGC CAAGATGCAG ACCAGGAGGC TTTTGTTACG TGGGCTGGAG
    CAGACCCCTG CCTCCAGTGT CGGGGACCTG GAGAGCTCTG TCACCGGGTC CATGATCAAT
    GGTTGGGGCT CTGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG CGTTAGTTCT
    TCTGATGGCT CTTTTTTCAC TGATGCCGAT TTTGCCCAGG CGGTCGCAGC AGCAGCGGAG
    TATGCTGGTC TGAAAGTGGC CCGGCGCCAA ATGCAGGATG CTGCTGGCCG CCGCCATTTT
    CATGCATCCC AGTGCCCTAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CGTGAGTGCT
    GCTGTAGTAC AGAAGGCCAG ACCAGCCAAG AAACAAAAAC ACCAGCCAGG ACATCTGCGC
    AGAGAAGCCT ACACAGATGA TCTTCCACCT CCCCCTGTGC CACCACCTGC TATCAAGTCA
    CCCACTGCCC AGTCCAAGGC CCAGCTGGAG GTTCGACCCG TGATGGTGCC AAAACTCCCT
    TCTATAGAAG CAAGAACAGA CAGATCATCA GATAGGAAAG GAGGCAATTA CAAGGGGAGA
    GAAGTATTGG ATGGAAGACA AGTTGCTGAT ATGCGACCAA ATCCAGGTGA TGCCAGAGAA
    GCACAGGAAC AGCCAAATGA CGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGAGACCTT
    CCACCAGCAA AGGCTCATCT CATCCAAGAG GATATTCTGC CTTACTGTAG ACCTACTTTT
    CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGTT CTATGTCATC AAGAGGATCA
    GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA GATGCAAGTT
    CTTGGAGGAT ATGAAAGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15498
    Clone ID Related Accession (Same CDS sequence) XM_005201171.4 , XM_005201171.3
    Accession Version XM_005201171.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGGGCA GCCCACCCCT
    TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGTGGA
    GACTCTGGCG AGAAGCACTG GAAGCCACCC GGACAGCAGA AACAGGAAGT GGCACCAATT
    CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGTGCAAA TGACACAATT
    GCTCCAACTA TCCCTTACAA CCAGTCATAT GACCAGAATA CTGGAGGATC CTACAACAGT
    TCAGACCGGG GAAGTAGTAC ATCAGGGAGT CAAGGGCACA AGAAAGGGGC AAGGACACCC
    AAAGTGCCAA AGCAAGGTGG CATGAACTGG GCAGACCTAC TGCCCCCTCC CCCAGCACAT
    CCTCCTCCTC ATAGCAACAG TGAAGAGTAC AGCATCTCTG TAGATGAAAG CTATGACCAA
    GAAATGCCAT GCCCCGTGCC CCCAGCACGG ATGTATCTGC AACAAGGTGA ACTAGAAGAG
    GAGGAAGATG AACGAGGTCC CACTCCCCCT GTGCGGGGAG CCGCCTCTTC TCCGGCTGCC
    GTGTCTTACA GCCATCAATC CACCGCCACT CTCACGCCCT CTCCACAGGA AGAACTCCAG
    CCCATGTTAC AGGATTGTCC CGAGGAGGTG GGCCATGGGC AGCACCAGCC TGACAGGAGA
    CGGCAGCCGG TGAGCCCACC TCCACCTCCA AGGCCGATTT CCCCACCCCA CACCTACGGC
    TACATTTCAG GACCCCTTGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC
    GAAGCCGACA TGGAAGTAGC CAAGATGCAG ACCAGGAGGC TTTTGTTACG TGGGCTGGAG
    CAGACCCCTG CCTCCAGTGT CGGGGACCTG GAGAGCTCTG TCACCGGGTC CATGATCAAT
    GGTTGGGGCT CTGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG CGTTAGTTCT
    TCTGATGGCT CTTTTTTCAC TGATGCCGAT TTTGCCCAGG CGGTCGCAGC AGCAGCGGAG
    TATGCTGGTC TGAAAGTGGC CCGGCGCCAA ATGCAGGATG CTGCTGGCCG CCGCCATTTT
    CATGCATCCC AGTGCCCTAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CGTGAGTGCT
    GCTGTAGTAC AGAAGGCCAG ACCAGCCAAG AAACAAAAAC ACCAGCCAGG ACATCTGCGC
    AGAGAAGCCT ACACAGATGA TCTTCCACCT CCCCCTGTGC CACCACCTGC TATCAAGTCA
    CCCACTGCCC AGTCCAAGGC CCAGCTGGAG GTTCGACCCG TGATGGTGCC AAAACTCCCT
    TCTATAGAAG CAAGAACAGA CAGATCATCA GATAGGAAAG GAGGCAATTA CAAGGGGAGA
    GAAGTATTGG ATGGAAGACA AGTTGCTGAT ATGCGACCAA ATCCAGGTGA TGCCAGAGAA
    GCACAGGAAC AGCCAAATGA CGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGAGACCTT
    CCACCAGCAA AGGCTCATCT CATCCAAGAG GATATTCTGC CTTACTGTAG ACCTACTTTT
    CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGTT CTATGTCATC AAGAGGATCA
    GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA GATGCAAGTT
    CTTGGAGGAT ATGAAAGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201228.1
    CDS297..5093
    Translation

    Target ORF information:

    RefSeq Version XM_005201171.3
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201171.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAAATGGA CACATGCTCC TTTTTTGGCC ATAATATCAC TCCTCAGCTT GTCAAAAAGT 
    CACTTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACG TAGAGAGGGG AAAAGACAAC
    GGGACGCCCA TCCCCACCTC CGATAACGAT GACAATTCGC TGGGCTATAC AGGCTCCCGT
    CTTCGTCAGG AAGATTTTCC ACCTCGTATT GTTGAACACC CGTCAGATCT AATTGTCTCA
    AAAGGAGAAC CTGCAACTTT GAACTGCAAA GCTGAAGGTC GCCCCACACC CACCATCGAA
    TGGTACAAAG GTGGGGAGAG AGTGGAGACA GACAAAGATG ACCCTCGCTC ACACCGAATG
    TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTGC ACGGACGAAA AAGTAGGCCT
    GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGTCACAAT
    GCATCGCTGG AGGTAGCCAT ACTACGGGAT GACTTCAGAC AAAACCCTTC CGATGTCATG
    GTGGCCGTGG GAGAGCCTGC AGTGATGGAA TGCCAGCCCC CACGAGGCCA TCCTGAGCCC
    ACCATTTCAT GGAAGAAAGA TGGCTCTCCA TTGGATGATA AAGATGAAAG AATAACTATA
    CGAGGAGGAA AGCTCATGAT TACTTACACC CGCAAAAGTG ATGCAGGCAA ATATGTTTGT
    GTTGGTACCA ACATGGTTGG GGAGCGTGAG AGTGAAGTAG CAGAGCTCAC TGTGTTAGAG
    AGACCATCCT TTGTGAAGAG ACCTAGTAAC TTGGCAGTCA CTGTGGATGA CAGTGCGGAA
    TTTAAATGTG AGGCCCGAGG TGACCCCGTG CCCACCGTAC GATGGAGGAA AGATGATGGG
    GAACTGCCCA AGTCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG
    ACAGCTGGTG ACATGGGGTC ATACACTTGT GTTGCAGAAA ACATGGTTGG AAAAGCTGAA
    GCATCAGCCA CTCTGACGGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC
    CGTGACCAGG TGGTTGCCTT GGGACGAACG GTCACTTTCC AGTGTGAAGC AACAGGAAAT
    CCCCAACCAG CTATCTTCTG GAGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATATCAG
    CCACCACAGT CATCCAGCCG TTTTTCAGTC TCCCAGACTG GTGACCTCAC TATTACTAAT
    GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TGAATGTTGC CGGAAGTATC
    ATCACAAAAG CATATTTGGA AGTTACCGAT GTGATTGCAG ACCGACCTCC CCCAGTCATT
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAGTGCT CAGCTGTGTG
    GCCACAGGCA GTCCAGTGCC CACAATTCTG TGGAGAAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GAATCAAACA GCTGGAGACC GGAGTACTAC AGATCCGATA TGCTAAGCTG
    GGTGATACTG GTCGATACAC GTGCATTGCA TCAACCCCCA GTGGTGAAGC AACGTGGAGT
    GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACCGACCCA
    AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCCG AAATACAGTA
    ACGTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CGACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACTGTAG CGGAGAATGT GAAAACAGAA
    ACATTTGCCA TTAAAGGACT CAAACCTAAT GCAATTTACC TTTTCCTTGT GAGGGCAGCC
    AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGACC CAGTGAAAAC ACAAGATGTT
    CCCCCAACCA GTCAGGGGGT GGATCACAAG CAGGTCCAGC GAGAACTGGG AAATGTTGTA
    CTGCATCTCC ACAACCCCAC CATCCTTTCC TCCTCTTCAA TCGAAGTGCA CTGGACAGTG
    GACCAACAGT CTCAGTATAT TCAAGGATAC AAAGTTCTTT ACCGCCCGTC TGGAGCCAAC
    CATGGTGAAT CCGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA CAGTGTGGTT
    ATCCCTGATC TCAAGAAAGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA
    TTTCAAGGAG CAGATAGTGA AATTAAATTT GCCAAAACCC TAGAAGAAGC ACCCAGTGCC
    CCACCTCAAA GTGTGACTGT ATCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT
    TGGCAGCCAC CTCCTGAAGA TACTCAAAAT GGAATGGTCC AAGAGTATAA GGTCTGGTGT
    CTGGGCAATG AAACGCGATA CCATATAAAC AAGACAGTAG ATGGTTCCAC CTTTTCTGTG
    GTTATTCCCT CACTGGTTCC TGGGATCCGA TATAGTGTAG AGGTGGCAGC CAGCACGGGG
    GCTGGGTCGG GGGTGAAGAG TGAGCCACAA TTCATCCAGT TAGATTCTCA TGGAAACCCC
    GTGTCACCTG AGGACCAAGT CAGCCTTGCT CAGCAGATCT CAGACGTAGT GAAGCAGCCG
    GCATTCATCG CAGGAATCGG GGCTGCCTGT TGGATCATCC TTATGGTTTT TAGCATCTGG
    CTCTATCGAC ACCGAAAGAA GAGAAATGGG CTGGCTAGTA CTTATGCGGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGAGGCGA AGCTGTGAGC
    AGTGGAGGGA GGCCAGGACT TCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTTGCA
    GACACATGGC CTAACACTGG CAACAATCAC AATGACTGCT CCATCAATTG CTGCACGGCA
    GGCAATGGGA ACAGTGACAG CAACTTGACC ACGTATAGTC GCCCAGGGCA GCCCACCCCT
    TATGCCACCA CCCAGCTCAT CCAGTCAAAC CTCAGCAACA ACATGAACAA TGGCAGTGGA
    GACTCTGGCG AGAAGCACTG GAAGCCACCC GGACAGCAGA AACAGGAAGT GGCACCAATT
    CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGTGCAAA TGACACAATT
    GCTCCAACTA TCCCTTACAA CCAGTCATAT GACCAGAATA CTGGAGGATC CTACAACAGT
    TCAGACCGGG GAAGTAGTAC ATCAGGGAGT CAAGGGCACA AGAAAGGGGC AAGGACACCC
    AAAGTGCCAA AGCAAGGTGG CATGAACTGG GCAGACCTAC TGCCCCCTCC CCCAGCACAT
    CCTCCTCCTC ATAGCAACAG TGAAGAGTAC AGCATCTCTG TAGATGAAAG CTATGACCAA
    GAAATGCCAT GCCCCGTGCC CCCAGCACGG ATGTATCTGC AACAAGGTGA ACTAGAAGAG
    GAGGAAGATG AACGAGGTCC CACTCCCCCT GTGCGGGGAG CCGCCTCTTC TCCGGCTGCC
    GTGTCTTACA GCCATCAATC CACCGCCACT CTCACGCCCT CTCCACAGGA AGAACTCCAG
    CCCATGTTAC AGGATTGTCC CGAGGAGGTG GGCCATGGGC AGCACCAGCC TGACAGGAGA
    CGGCAGCCGG TGAGCCCACC TCCACCTCCA AGGCCGATTT CCCCACCCCA CACCTACGGC
    TACATTTCAG GACCCCTTGT CTCAGATATG GATACGGATG CGCCAGAAGA GGAAGAAGAC
    GAAGCCGACA TGGAAGTAGC CAAGATGCAG ACCAGGAGGC TTTTGTTACG TGGGCTGGAG
    CAGACCCCTG CCTCCAGTGT CGGGGACCTG GAGAGCTCTG TCACCGGGTC CATGATCAAT
    GGTTGGGGCT CTGCCTCAGA GGAGGACAAC ATTTCCAGCG GACGCTCCAG CGTTAGTTCT
    TCTGATGGCT CTTTTTTCAC TGATGCCGAT TTTGCCCAGG CGGTCGCAGC AGCAGCGGAG
    TATGCTGGTC TGAAAGTGGC CCGGCGCCAA ATGCAGGATG CTGCTGGCCG CCGCCATTTT
    CATGCATCCC AGTGCCCTAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CGTGAGTGCT
    GCTGTAGTAC AGAAGGCCAG ACCAGCCAAG AAACAAAAAC ACCAGCCAGG ACATCTGCGC
    AGAGAAGCCT ACACAGATGA TCTTCCACCT CCCCCTGTGC CACCACCTGC TATCAAGTCA
    CCCACTGCCC AGTCCAAGGC CCAGCTGGAG GTTCGACCCG TGATGGTGCC AAAACTCCCT
    TCTATAGAAG CAAGAACAGA CAGATCATCA GATAGGAAAG GAGGCAATTA CAAGGGGAGA
    GAAGTATTGG ATGGAAGACA AGTTGCTGAT ATGCGACCAA ATCCAGGTGA TGCCAGAGAA
    GCACAGGAAC AGCCAAATGA CGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGAGACCTT
    CCACCAGCAA AGGCTCATCT CATCCAAGAG GATATTCTGC CTTACTGTAG ACCTACTTTT
    CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGTT CTATGTCATC AAGAGGATCA
    GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA GATGCAAGTT
    CTTGGAGGAT ATGAAAGAGA AGATAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15499
    Clone ID Related Accession (Same CDS sequence) XM_005201172.3 , XM_005201172.4
    Accession Version XM_005201172.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC
    AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC
    TTGACCACGT ATAGTCGCCC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG
    CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG
    TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA
    GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG
    AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA
    GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA
    GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT
    CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC
    GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG
    GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA
    CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA
    GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG
    ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG
    GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG
    GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT
    GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG
    CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC
    ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA
    GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT
    CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG
    CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA
    TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT
    GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG
    AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC
    CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201229.1
    CDS1..4653
    Translation

    Target ORF information:

    RefSeq Version XM_005201172.3
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201172.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC
    AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC
    TTGACCACGT ATAGTCGCCC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG
    CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG
    TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA
    GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG
    AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA
    GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA
    GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT
    CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC
    GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG
    GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA
    CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA
    GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG
    ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG
    GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG
    GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT
    GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG
    CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC
    ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA
    GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT
    CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG
    CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA
    TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT
    GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG
    AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC
    CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15499
    Clone ID Related Accession (Same CDS sequence) XM_005201172.3 , XM_005201172.4
    Accession Version XM_005201172.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201172.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC
    AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC
    TTGACCACGT ATAGTCGCCC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG
    CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG
    TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA
    GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG
    AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA
    GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA
    GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT
    CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC
    GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG
    GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA
    CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA
    GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG
    ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG
    GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG
    GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT
    GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG
    CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC
    ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA
    GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT
    CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG
    CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA
    TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT
    GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG
    AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC
    CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201229.1
    CDS1112..5764
    Translation

    Target ORF information:

    RefSeq Version XM_005201172.4
    Organism Bos taurus(cattle)
    Definition Bos taurus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201172.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCTCGT ATTGTTGAAC ACCCGTCAGA TCTAATTGTC
    TCAAAAGGAG AACCTGCAAC TTTGAACTGC AAAGCTGAAG GTCGCCCCAC ACCCACCATC
    GAATGGTACA AAGGTGGGGA GAGAGTGGAG ACAGACAAAG ATGACCCTCG CTCACACCGA
    ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TGCACGGACG AAAAAGTAGG
    CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGTCAC
    AATGCATCGC TGGAGGTAGC CATACTACGG GATGACTTCA GACAAAACCC TTCCGATGTC
    ATGGTGGCCG TGGGAGAGCC TGCAGTGATG GAATGCCAGC CCCCACGAGG CCATCCTGAG
    CCCACCATTT CATGGAAGAA AGATGGCTCT CCATTGGATG ATAAAGATGA AAGAATAACT
    ATACGAGGAG GAAAGCTCAT GATTACTTAC ACCCGCAAAA GTGATGCAGG CAAATATGTT
    TGTGTTGGTA CCAACATGGT TGGGGAGCGT GAGAGTGAAG TAGCAGAGCT CACTGTGTTA
    GAGAGACCAT CCTTTGTGAA GAGACCTAGT AACTTGGCAG TCACTGTGGA TGACAGTGCG
    GAATTTAAAT GTGAGGCCCG AGGTGACCCC GTGCCCACCG TACGATGGAG GAAAGATGAT
    GGGGAACTGC CCAAGTCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG
    GTGACAGCTG GTGACATGGG GTCATACACT TGTGTTGCAG AAAACATGGT TGGAAAAGCT
    GAAGCATCAG CCACTCTGAC GGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA
    CCCCGTGACC AGGTGGTTGC CTTGGGACGA ACGGTCACTT TCCAGTGTGA AGCAACAGGA
    AATCCCCAAC CAGCTATCTT CTGGAGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAT
    CAGCCACCAC AGTCATCCAG CCGTTTTTCA GTCTCCCAGA CTGGTGACCT CACTATTACT
    AATGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTGAATGT TGCCGGAAGT
    ATCATCACAA AAGCATATTT GGAAGTTACC GATGTGATTG CAGACCGACC TCCCCCAGTC
    ATTCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAGT GCTCAGCTGT
    GTGGCCACAG GCAGTCCAGT GCCCACAATT CTGTGGAGAA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACCGGAGTAC TACAGATCCG ATATGCTAAG
    CTGGGTGATA CTGGTCGATA CACGTGCATT GCATCAACCC CCAGTGGTGA AGCAACGTGG
    AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACCGAC
    CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CCGAAATACA
    GTAACGTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCGACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACTG TAGCGGAGAA TGTGAAAACA
    GAAACATTTG CCATTAAAGG ACTCAAACCT AATGCAATTT ACCTTTTCCT TGTGAGGGCA
    GCCAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ACCCAGTGAA AACACAAGAT
    GTTCCCCCAA CCAGTCAGGG GGTGGATCAC AAGCAGGTCC AGCGAGAACT GGGAAATGTT
    GTACTGCATC TCCACAACCC CACCATCCTT TCCTCCTCTT CAATCGAAGT GCACTGGACA
    GTGGACCAAC AGTCTCAGTA TATTCAAGGA TACAAAGTTC TTTACCGCCC GTCTGGAGCC
    AACCATGGTG AATCCGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAACAGTGTG
    GTTATCCCTG ATCTCAAGAA AGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT
    GAATTTCAAG GAGCAGATAG TGAAATTAAA TTTGCCAAAA CCCTAGAAGA AGCACCCAGT
    GCCCCACCTC AAAGTGTGAC TGTATCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT
    AGTTGGCAGC CACCTCCTGA AGATACTCAA AATGGAATGG TCCAAGAGTA TAAGGTCTGG
    TGTCTGGGCA ATGAAACGCG ATACCATATA AACAAGACAG TAGATGGTTC CACCTTTTCT
    GTGGTTATTC CCTCACTGGT TCCTGGGATC CGATATAGTG TAGAGGTGGC AGCCAGCACG
    GGGGCTGGGT CGGGGGTGAA GAGTGAGCCA CAATTCATCC AGTTAGATTC TCATGGAAAC
    CCCGTGTCAC CTGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCAGACGT AGTGAAGCAG
    CCGGCATTCA TCGCAGGAAT CGGGGCTGCC TGTTGGATCA TCCTTATGGT TTTTAGCATC
    TGGCTCTATC GACACCGAAA GAAGAGAAAT GGGCTGGCTA GTACTTATGC GGGTATCAGA
    AAAGTAACTT ATCAGAGAGG AGGCGAAGCT GTGAGCAGTG GAGGGAGGCC AGGACTTCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTTGCAGACA CATGGCCTAA CACTGGCAAC
    AATCACAATG ACTGCTCCAT CAATTGCTGC ACGGCAGGCA ATGGGAACAG TGACAGCAAC
    TTGACCACGT ATAGTCGCCC AGGGCAGCCC ACCCCTTATG CCACCACCCA GCTCATCCAG
    TCAAACCTCA GCAACAACAT GAACAATGGC AGTGGAGACT CTGGCGAGAA GCACTGGAAG
    CCACCCGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAACTGAACA AAGATTATCG TGCAAATGAC ACAATTGCTC CAACTATCCC TTACAACCAG
    TCATATGACC AGAATACTGG AGGATCCTAC AACAGTTCAG ACCGGGGAAG TAGTACATCA
    GGGAGTCAAG GGCACAAGAA AGGGGCAAGG ACACCCAAAG TGCCAAAGCA AGGTGGCATG
    AACTGGGCAG ACCTACTGCC CCCTCCCCCA GCACATCCTC CTCCTCATAG CAACAGTGAA
    GAGTACAGCA TCTCTGTAGA TGAAAGCTAT GACCAAGAAA TGCCATGCCC CGTGCCCCCA
    GCACGGATGT ATCTGCAACA AGGTGAACTA GAAGAGGAGG AAGATGAACG AGGTCCCACT
    CCCCCTGTGC GGGGAGCCGC CTCTTCTCCG GCTGCCGTGT CTTACAGCCA TCAATCCACC
    GCCACTCTCA CGCCCTCTCC ACAGGAAGAA CTCCAGCCCA TGTTACAGGA TTGTCCCGAG
    GAGGTGGGCC ATGGGCAGCA CCAGCCTGAC AGGAGACGGC AGCCGGTGAG CCCACCTCCA
    CCTCCAAGGC CGATTTCCCC ACCCCACACC TACGGCTACA TTTCAGGACC CCTTGTCTCA
    GATATGGATA CGGATGCGCC AGAAGAGGAA GAAGACGAAG CCGACATGGA AGTAGCCAAG
    ATGCAGACCA GGAGGCTTTT GTTACGTGGG CTGGAGCAGA CCCCTGCCTC CAGTGTCGGG
    GACCTGGAGA GCTCTGTCAC CGGGTCCATG ATCAATGGTT GGGGCTCTGC CTCAGAGGAG
    GACAACATTT CCAGCGGACG CTCCAGCGTT AGTTCTTCTG ATGGCTCTTT TTTCACTGAT
    GCCGATTTTG CCCAGGCGGT CGCAGCAGCA GCGGAGTATG CTGGTCTGAA AGTGGCCCGG
    CGCCAAATGC AGGATGCTGC TGGCCGCCGC CATTTTCATG CATCCCAGTG CCCTAGACCC
    ACAAGTCCTG TTTCTACAGA CAGCAACGTG AGTGCTGCTG TAGTACAGAA GGCCAGACCA
    GCCAAGAAAC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT
    CCACCTCCCC CTGTGCCACC ACCTGCTATC AAGTCACCCA CTGCCCAGTC CAAGGCCCAG
    CTGGAGGTTC GACCCGTGAT GGTGCCAAAA CTCCCTTCTA TAGAAGCAAG AACAGACAGA
    TCATCAGATA GGAAAGGAGG CAATTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT
    GCTGATATGC GACCAAATCC AGGTGATGCC AGAGAAGCAC AGGAACAGCC AAATGACGGG
    AAAGGACGAG GAAACAAGGC AGCAAAACGA GACCTTCCAC CAGCAAAGGC TCATCTCATC
    CAAGAGGATA TTCTGCCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT
    CCTAGTTCTT CAAGTTCTAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA
    AATGTAGGTC GAAGAAATAT TGCAGAGATG CAAGTTCTTG GAGGATATGA AAGAGAAGAT
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.