NALCN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NALCN
Entrez Gene ID	536103
Full Name	sodium leak channel, non-selective
General protein information	Preferred Names sodium leak channel, non-selective Names sodium leak channel non-selective protein LOW QUALITY PROTEIN: sodium leak channel non-selective protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002691980.5	XP_002692026.1	sodium leak channel non-selective protein isoform X1
XM_025000163.1	XP_024855931.1	sodium leak channel non-selective protein
XM_025000161.1	XP_024855929.1	sodium leak channel non-selective protein
XM_025000162.1	XP_024855930.1	sodium leak channel non-selective protein
XM_015465825.1	XP_015321311.1	LOW QUALITY PROTEIN: sodium leak channel non-selective protein isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NALCN	XP_001149573.1
Canis lupus familiaris (dog)	NALCN	XP_542654.4
Bos taurus (cattle)	NALCN	XP_002692026.1
Rattus norvegicus (Norway rat)	Nalcn	NP_705894.1
Drosophila melanogaster (fruit fly)	na	NP_001096981.1
Homo sapiens (human)	NALCN	NP_443099.1
Caenorhabditis elegans (roundworm)	nca-2	NP_498054.2
Mus musculus (house mouse)	Nalcn	NP_796367.3
Caenorhabditis elegans (roundworm)	unc-77	NP_741413.2
Gallus gallus (chicken)	NALCN	XP_416967.2
Danio rerio (zebrafish)	nalcn	NP_001017549.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003921	XP_318373.5
Xenopus tropicalis (tropical clawed frog)	nalcn	XP_002937125.2

Gene Ontology

The following NALCN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NALCN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa221344	XM_025000162.1 Latest version!	Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221344	XM_025000163.1 Latest version!	Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221344	XM_025000161.1 Latest version!	Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa94746	XM_015465825.1 Latest version!	Bos taurus sodium leak channel, non selective (NALCN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa07356	XM_002691980.5 Latest version!	Bos taurus sodium leak channel, non selective (NALCN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NALCN ( XM_025000163.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855931.1 Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.

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CloneID	OBa221344
Clone ID Related Accession (Same CDS sequence)	XM_025000163.1 , XM_025000161.1 , XM_025000162.1
Accession Version	XM_025000163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium leak channel non-selective protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG 
TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG 
TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG 
AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC 
CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC 
GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG 
CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC 
GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC 
CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855931.1
CDS	263..5479
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQQMMRSSVFHMFILSMVTVDVIVAASNYHKGESFKRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHAYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPSTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLLVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPMHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIMAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIISLKQSQQQELKRILNPPSIETTQPSEDLKTNSQDNSVQPESSSQQQLLSPTLSDRGGGRQDAAEAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_025000163.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000163.1

ORF Insert Sequence:

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG 
TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG 
TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG 
AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC 
CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC 
GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG 
CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC 
GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC 
CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_025000161.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855929.1 Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.

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CloneID	OBa221344
Clone ID Related Accession (Same CDS sequence)	XM_025000163.1 , XM_025000161.1 , XM_025000162.1
Accession Version	XM_025000161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium leak channel non-selective protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG 
TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG 
TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG 
AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC 
CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC 
GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG 
CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC 
GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC 
CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855929.1
CDS	119..5335
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQQMMRSSVFHMFILSMVTVDVIVAASNYHKGESFKRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHAYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPSTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLLVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPMHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIMAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIISLKQSQQQELKRILNPPSIETTQPSEDLKTNSQDNSVQPESSSQQQLLSPTLSDRGGGRQDAAEAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_025000161.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000161.1

ORF Insert Sequence:

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG 
TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG 
TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG 
AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC 
CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC 
GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG 
CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC 
GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC 
CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_025000162.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855930.1 Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa221344
Clone ID Related Accession (Same CDS sequence)	XM_025000163.1 , XM_025000161.1 , XM_025000162.1
Accession Version	XM_025000162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium leak channel non-selective protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG 
TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG 
TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG 
AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC 
CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC 
GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG 
CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC 
GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC 
CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855930.1
CDS	145..5361
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQQMMRSSVFHMFILSMVTVDVIVAASNYHKGESFKRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHAYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPSTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLLVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPMHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIMAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIISLKQSQQQELKRILNPPSIETTQPSEDLKTNSQDNSVQPESSSQQQLLSPTLSDRGGGRQDAAEAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_025000162.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000162.1

ORF Insert Sequence:

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG 
TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG 
TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG 
AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC 
CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC 
GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG 
CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC 
GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC 
CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_015465825.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321311.1 Bos taurus sodium leak channel, non selective (NALCN), transcript variant X2, mRNA.

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CloneID	OBa94746
Clone ID Related Accession (Same CDS sequence)	XM_015465825.1
Accession Version	XM_015465825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGT 
NCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG 
TACACCATCG AGGAGGAGNG TGCCAAGCAG ACCATCCGCA TGTGGCTGAA AGAAGTGCCT 
GAAGGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC 
CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC 
GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG 
CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC 
GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC 
CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321311.1
CDS	137..5353
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQQMMRSSVFHMFILSMVTVDVIVAASNYHKGESFKRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHAYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPSTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLLVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPMHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIMAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRnullVDIRKSLQLEELLAREQLEYTIEEEnullAKQTIRMWLKEVPEGIRAKQQQSCSIIISLKQSQQQELKRILNPPSIETTQPSEDLKTNSQDNSVQPESSSQQQLLSPTLSDRGGGRQDAAEAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_015465825.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium leak channel, non selective (NALCN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465825.1

ORF Insert Sequence:

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGT 
NCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG 
TACACCATCG AGGAGGAGNG TGCCAAGCAG ACCATCCGCA TGTGGCTGAA AGAAGTGCCT 
GAAGGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC 
CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC 
GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG 
CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC 
GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC 
CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_002691980.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002692026.1 Bos taurus sodium leak channel, non selective (NALCN), transcript variant X1, mRNA.

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CloneID	OBa07356
Clone ID Related Accession (Same CDS sequence)	XM_002691980.5
Accession Version	XM_002691980.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691980.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGGTACGG GAAACAGCAG 
CAGTCCTGTA GCATCATCAT CAGCCTGAAG CAGAGCCAAC AGCAGGAGTT GAAGCGGATC 
CTCAACCCGC CCAGCATCGA GACCACGCAG CCCAGCGAAG ACCTGAAGAC CAACAGTCAG 
GACAACAGTG TGCAACCCGA GTCGAGCAGC CAGCAGCAAC TCCTGAGCCC CACCCTCTCG 
GACCGGGGAG GGGGTCGCCA GGACGCGGCC GAGGCCGGGA AGCCCCAGAG GAAGTTCGGG 
CAGTGGCGCC TGCCCTCTGC ACCAAAACCA ATAAGCCACT CGGTGTCTTC GGTCAACTTA 
CGGTTTGGAG GGCGGACCAC AATGAAATCT GTCGTGTGCA AAATGAACCC CATGACGGAC 
ACCGCTTCCT GTGGCTCTGA GGTTAAGAAG TGGTGGACCC GGCAGCTGAC CGTGGAGAGT 
GATGAAAGCG GAGATGACCT TCTGGACATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002692026.1
CDS	137..5209
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQQMMRSSVFHMFILSMVTVDVIVAASNYHKGESFKRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHAYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPSTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLLVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPMHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIMAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLRYGKQQQSCSIIISLKQSQQQELKRILNPPSIETTQPSEDLKTNSQDNSVQPESSSQQQLLSPTLSDRGGGRQDAAEAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_002691980.5
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium leak channel, non selective (NALCN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691980.5

ORF Insert Sequence:

ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC 
TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT 
CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT 
TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC 
ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT 
GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT 
GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC 
AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC 
CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA 
GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC 
TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC 
ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC 
AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG 
CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG 
ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG 
GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA 
ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT 
CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT 
GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT 
CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG 
GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC 
CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC 
GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA 
CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC 
CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC 
AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA 
GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC 
CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA 
GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG 
GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG 
GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA 
TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC 
TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC 
ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT 
GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT 
GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG 
CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC 
TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA 
TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC 
GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG 
AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT 
CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC 
CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT 
CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC 
ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG 
ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC 
ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA 
CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG 
GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG 
AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT 
GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC 
CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG 
CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGGTACGG GAAACAGCAG 
CAGTCCTGTA GCATCATCAT CAGCCTGAAG CAGAGCCAAC AGCAGGAGTT GAAGCGGATC 
CTCAACCCGC CCAGCATCGA GACCACGCAG CCCAGCGAAG ACCTGAAGAC CAACAGTCAG 
GACAACAGTG TGCAACCCGA GTCGAGCAGC CAGCAGCAAC TCCTGAGCCC CACCCTCTCG 
GACCGGGGAG GGGGTCGCCA GGACGCGGCC GAGGCCGGGA AGCCCCAGAG GAAGTTCGGG 
CAGTGGCGCC TGCCCTCTGC ACCAAAACCA ATAAGCCACT CGGTGTCTTC GGTCAACTTA 
CGGTTTGGAG GGCGGACCAC AATGAAATCT GTCGTGTGCA AAATGAACCC CATGACGGAC 
ACCGCTTCCT GTGGCTCTGA GGTTAAGAAG TGGTGGACCC GGCAGCTGAC CGTGGAGAGT 
GATGAAAGCG GAGATGACCT TCTGGACATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NALCN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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