NALCN cDNA ORF clone, Bos taurus(cattle)

The following NALCN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NALCN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221344 XM_025000162.1
Latest version!
Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221344 XM_025000163.1
Latest version!
Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221344 XM_025000161.1
Latest version!
Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa94746 XM_015465825.1
Latest version!
Bos taurus sodium leak channel, non selective (NALCN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa07356 XM_002691980.5
Latest version!
Bos taurus sodium leak channel, non selective (NALCN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221344
    Clone ID Related Accession (Same CDS sequence) XM_025000163.1 , XM_025000161.1 , XM_025000162.1
    Accession Version XM_025000163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sodium leak channel non-selective protein
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG
    TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG
    TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG
    AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC
    CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC
    GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG
    CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC
    GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC
    CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG
    TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG
    ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855931.1
    CDS263..5479
    Translation

    Target ORF information:

    RefSeq Version XM_025000163.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG
    TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG
    TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG
    AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC
    CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC
    GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG
    CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC
    GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC
    CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG
    TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG
    ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221344
    Clone ID Related Accession (Same CDS sequence) XM_025000163.1 , XM_025000161.1 , XM_025000162.1
    Accession Version XM_025000161.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sodium leak channel non-selective protein
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG
    TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG
    TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG
    AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC
    CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC
    GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG
    CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC
    GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC
    CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG
    TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG
    ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855929.1
    CDS119..5335
    Translation

    Target ORF information:

    RefSeq Version XM_025000161.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG
    TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG
    TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG
    AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC
    CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC
    GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG
    CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC
    GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC
    CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG
    TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG
    ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221344
    Clone ID Related Accession (Same CDS sequence) XM_025000163.1 , XM_025000161.1 , XM_025000162.1
    Accession Version XM_025000162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sodium leak channel non-selective protein
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG
    TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG
    TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG
    AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC
    CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC
    GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG
    CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC
    GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC
    CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG
    TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG
    ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855930.1
    CDS145..5361
    Translation

    Target ORF information:

    RefSeq Version XM_025000162.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGG
    TCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG
    TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCCTG
    AAGCGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC
    CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC
    GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG
    CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC
    GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC
    CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG
    TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG
    ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94746
    Clone ID Related Accession (Same CDS sequence) XM_015465825.1
    Accession Version XM_015465825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: sodium leak channel non-selective protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGT
    NCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG
    TACACCATCG AGGAGGAGNG TGCCAAGCAG ACCATCCGCA TGTGGCTGAA AGAAGTGCCT
    GAAGGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC
    CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC
    GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG
    CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC
    GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC
    CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG
    TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG
    ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321311.1
    CDS137..5353
    Translation

    Target ORF information:

    RefSeq Version XM_015465825.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sodium leak channel, non selective (NALCN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGCATGCT CTCCTACCGT
    NCTGTGGACA TCCGCAAGAG TTTGCAGCTG GAAGAGCTGC TGGCCAGGGA GCAGTTGGAG
    TACACCATCG AGGAGGAGNG TGCCAAGCAG ACCATCCGCA TGTGGCTGAA AGAAGTGCCT
    GAAGGCATCC GAGCCAAACA GCAGCAGTCC TGTAGCATCA TCATCAGCCT GAAGCAGAGC
    CAACAGCAGG AGTTGAAGCG GATCCTCAAC CCGCCCAGCA TCGAGACCAC GCAGCCCAGC
    GAAGACCTGA AGACCAACAG TCAGGACAAC AGTGTGCAAC CCGAGTCGAG CAGCCAGCAG
    CAACTCCTGA GCCCCACCCT CTCGGACCGG GGAGGGGGTC GCCAGGACGC GGCCGAGGCC
    GGGAAGCCCC AGAGGAAGTT CGGGCAGTGG CGCCTGCCCT CTGCACCAAA ACCAATAAGC
    CACTCGGTGT CTTCGGTCAA CTTACGGTTT GGAGGGCGGA CCACAATGAA ATCTGTCGTG
    TGCAAAATGA ACCCCATGAC GGACACCGCT TCCTGTGGCT CTGAGGTTAA GAAGTGGTGG
    ACCCGGCAGC TGACCGTGGA GAGTGATGAA AGCGGAGATG ACCTTCTGGA CATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07356
    Clone ID Related Accession (Same CDS sequence) XM_002691980.5
    Accession Version XM_002691980.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium leak channel non-selective protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691980.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGGTACGG GAAACAGCAG
    CAGTCCTGTA GCATCATCAT CAGCCTGAAG CAGAGCCAAC AGCAGGAGTT GAAGCGGATC
    CTCAACCCGC CCAGCATCGA GACCACGCAG CCCAGCGAAG ACCTGAAGAC CAACAGTCAG
    GACAACAGTG TGCAACCCGA GTCGAGCAGC CAGCAGCAAC TCCTGAGCCC CACCCTCTCG
    GACCGGGGAG GGGGTCGCCA GGACGCGGCC GAGGCCGGGA AGCCCCAGAG GAAGTTCGGG
    CAGTGGCGCC TGCCCTCTGC ACCAAAACCA ATAAGCCACT CGGTGTCTTC GGTCAACTTA
    CGGTTTGGAG GGCGGACCAC AATGAAATCT GTCGTGTGCA AAATGAACCC CATGACGGAC
    ACCGCTTCCT GTGGCTCTGA GGTTAAGAAG TGGTGGACCC GGCAGCTGAC CGTGGAGAGT
    GATGAAAGCG GAGATGACCT TCTGGACATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692026.1
    CDS137..5209
    Translation

    Target ORF information:

    RefSeq Version XM_002691980.5
    Organism Bos taurus(cattle)
    Definition Bos taurus sodium leak channel, non selective (NALCN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691980.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTCAAAC GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CGGTTACTGA CTTTGGTCCC 
    GATGAATCTC TGTCGGACAA CGCAGACATC CTCTGGATTA ACAAGCCATG GGTTCACTCT
    CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTCT GTATGAACAC GCCCATGACC
    TTCGAACACT ACCCTCCTCT GCAGTATGTG ACCTTCACCC TGGACACTCT GTTGATGTTT
    CTCTACACAG CAGAGATGAT CGCCAAAATG CACATCCGGG GGATTGTCAA GGGGGATAGT
    TCCTACGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT
    TCATTGGTGT TACAGGTGTT TGAAATCGCT GACATAGTTG ATCAGATGTC TCCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATCCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAATATA TTAAAGCGAT CAGGAGAACA AATATGGAGT
    GTTTCCATTT TCCTCCTTTT CTTTCTGCTT CTTTATGGCA TTTTAGGAGT TCAGATGTTT
    GGGACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAATGT AACCTGGAAC
    AGTTTAGCCA TCCCAGATAC ACACTGCTCC CCAGAACTGG AAGAAGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ACGAGATAGG AACCAGCATC TTCACTGTGT ACGAGGCCGC TTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CCGAGCCATC GACAGCTTTC CCCGCTGGCG TTCCTACTTC
    TACTTCATCA CCCTGATCTT CTTCCTGGCC TGGCTTGTTA AGAACGTGTT CATCGCTGTC
    ATTATAGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AGATGTGGGG GTCAAGAAGC
    AGCACTACTT CCACGGCCAC CACGCAGATG TTTCATGAAG ATGCTGCTGG CGGGTGGCAG
    CTGGTAGCCG TGGATGTCAA CAAGCCCCAG GGACGAGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCGT CCGTCTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG
    GCCGCCAGCA ACTATCACAA AGGAGAAAGC TTCAAGAGAC AGTATGACGA GTTCTACCTG
    GCAGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG
    GGATTCACTG GATATATTAG CTCATCTCTC CACAAATTCG AGCTGCTACT CGTAATTGGA
    ACCACTCTTC ATGCATATCC AGACCTTTAT CATTCCCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GGCTTATTAA AATCTCACCT GCATTAGAAG ACTTTGTGTA CAAGATCTTT
    GGTCCTGGAA AAAAGCTGGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCGGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAGC TGGATAGATT CACGACATTT
    CCAAGGGCAT TCATGTCTAT GTTCCAGATT CTTACCCAGG AAGGATGGGT TGATGTCATG
    GACCAAACAC TAAATGCCGT GGGACACATG TGGGCGCCTG TGGTTGCCAT CTACTTTATC
    CTCTATCACC TCTTCGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC
    AACTTAGAGC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAC
    GCAGACACCA AGGAAAAGCT CCCTTTGCGA CTTCGCATCT TTGAAAAATT TCCGAACAGA
    CCGCAAATGG TGAAGATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CATCTTCCTC ATCCTGCGAT CACTCCAAAC GGTCTGCGAT TGAGGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCA GTCTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAAAAGGAGA CTGCAGTCAC GAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC GGCGAAGGAG AGGTCCATTC TTAGCGTGCA ACACCACATC
    CGCCAAGAGC GCAGGTCATT AAGACACGGA TCAAACAGCC AAAGGATCAG CAGGGGGAAA
    TCTCTTGAAA CTCTGACTCA AGATCATTCC AACACGGTGA GATACAGAAA TGCACAAAGA
    GAAGACAGTG AGATAAAGAT GATCCAGGAG AAAAAGGAGC AAGCAGAAAT GAAAAGGAAG
    GTGCAGGAAG AGGAGCTCAG AGAGAACCAC CCCTACTTCG ACAAGCCCCT GTTCATCGTG
    GGGCGAGAGC ACAGGTTCAG GAACTTCTGC CGCGTGGTGG TCCGGGCTCG CTTCAACGCA
    TCTAAAACAG ATCCGGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAC
    TTACTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCCTGC
    ATCTCCATGA TGTTCGAATC CCCCTTTCGA AGAGTGATGC ACACACCTAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCCTATTTT TCACTCCGAC CGCTGTCATC AGGGACTTTG GTGGCGTAAT GGACATATTT
    ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCCC AAAACGTGCC TGCGGAGTCT
    GGAGCTCAGC TCTTACTGGT TCTCCGCTGC CTGCGACCCC TGCGCATATT CAAGCTGGTG
    CCTCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TTAAGGAAAT TTTTCTGGTC
    TCCATTCTCT TGCTGACCTT AATGCTAGTT TTTGCAAGTT TTGGAGTTCA GCTTTTTGCT
    GGAAAACTGG CCAAATGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG TAATGGAATA
    TTCAGAATTA ATGTGAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTTGACAAC
    GTGGGCAATG CAATGCTGGC GTTGTTTGAA GTTCTGTCCT TGAAAGGCTG GGTGGAAGTG
    AGGGACGTTA TTATTCATCG TGTGGGGCCG ATGCATGGAA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TGGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACGGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TTTGAAGAGT
    CGACTGAAGA TAGCACAGCC TCTTCATCTG CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ACGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTGGTC
    CTGGCTCAGT CGGTGTTGCT GTCCGTCAAG TGGGACGTCG AGGACCCGGT GACTGTCCCT
    CTGGCCACCA TGTCGGTCGT CTTCACGTTC ATCTTTGTTC TGGAGGTTAC GATGAAGATC
    ATGGCAATGT CTCCTGCTGG CTTCTGGCAA AGCAGGAGAA ATCGGTACGA TCTGCTGGTG
    ACGTCACTGG GCGTTGTCTG GGTGGTCCTT CACTTCGCCC TCCTGAACGC GTACACCTAC
    ATGATGGGAG CGTGTGTGAT TGTCTTCCGG TTTTTCTCCA TCTGCGGGAA GCATGTAACA
    CTGAAGATGC TTCTCCTGAC GGTGGTTGTC AGCATGTATA AAAGCTTCTT TATCATAGTG
    GGCATGTTCC TATTGCTGCT GTGTTATGCT TTCGCTGGGG TTGTCTTATT TGGTACTGTG
    AAATATGGAG AAAACATTAA CAGGCATGCA AACTTTTCTT CAGCTGGCAA AGCTATTACT
    GTACTGTTCC GAATTGTCAC AGGAGAAGAT TGGAATAAGA TCATGCACGA CTGTATGGTC
    CAGCCCCCCT TTTGTACTCC CGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAAGACCAG
    CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA
    AGAGAGGGGG TGATCCCAAC CTTCCGCGTG AAGTTCCTGC TGCGGCTGCT GCGAGGCAGG
    CTGGAGGTGG ACCTGGACAA GGACAAGCTT CTGTTCAAGC ACATGTGCTA CGAGATGGAG
    CGGCTGCACA ACGGCGGTGA CGTCACCTTC CACGACGTGC TCAGGTACGG GAAACAGCAG
    CAGTCCTGTA GCATCATCAT CAGCCTGAAG CAGAGCCAAC AGCAGGAGTT GAAGCGGATC
    CTCAACCCGC CCAGCATCGA GACCACGCAG CCCAGCGAAG ACCTGAAGAC CAACAGTCAG
    GACAACAGTG TGCAACCCGA GTCGAGCAGC CAGCAGCAAC TCCTGAGCCC CACCCTCTCG
    GACCGGGGAG GGGGTCGCCA GGACGCGGCC GAGGCCGGGA AGCCCCAGAG GAAGTTCGGG
    CAGTGGCGCC TGCCCTCTGC ACCAAAACCA ATAAGCCACT CGGTGTCTTC GGTCAACTTA
    CGGTTTGGAG GGCGGACCAC AATGAAATCT GTCGTGTGCA AAATGAACCC CATGACGGAC
    ACCGCTTCCT GTGGCTCTGA GGTTAAGAAG TGGTGGACCC GGCAGCTGAC CGTGGAGAGT
    GATGAAAGCG GAGATGACCT TCTGGACATT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.