Nalcn gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
NM_177393.4	NP_796367.3	sodium leak channel non-selective protein
XM_017316064.1	XP_017171553.1	sodium leak channel non-selective protein isoform X4
XM_017316066.2	XP_017171555.1	sodium leak channel non-selective protein isoform X6
XM_017316066.1	XP_017171555.1	sodium leak channel non-selective protein isoform X6
XM_011245094.3	XP_011243396.1	sodium leak channel non-selective protein isoform X2
XM_017316064.2	XP_017171553.1	sodium leak channel non-selective protein isoform X4
XM_017316062.1	XP_017171551.1	sodium leak channel non-selective protein isoform X1
XM_011245094.2	XP_011243396.1	sodium leak channel non-selective protein isoform X2
XM_017316063.1	XP_017171552.1	sodium leak channel non-selective protein isoform X3
XM_017316062.2	XP_017171551.1	sodium leak channel non-selective protein isoform X1
XM_017316063.2	XP_017171552.1	sodium leak channel non-selective protein isoform X3
XM_030247866.1	XP_030103726.1	sodium leak channel non-selective protein isoform X5
XM_017316065.1	XP_017171554.1	sodium leak channel non-selective protein isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu78793	XM_017316066.2 Latest version!	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74946	XM_017316062.2 Latest version!	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu74947	XM_011245094.3 Latest version!	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu74948	XM_017316063.2 Latest version!	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu74949	XM_017316064.2 Latest version!	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78792	XM_017316065.1 Latest version!	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu86440	XM_030247866.1 Latest version!	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20803	NM_177393.4 Latest version!	Mus musculus sodium leak channel, non-selective (Nalcn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1280.30 $1829.00
View Old Accession Versions >>
OMu78793	XM_017316066.1	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74949	XM_017316064.1	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu74947	XM_011245094.2	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu74946	XM_017316062.1	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu74948	XM_017316063.1	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

Nalcn ( XM_017316066.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171555.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu78793
Clone ID Related Accession (Same CDS sequence)	XM_017316066.2 , XM_017316066.1
Accession Version	XM_017316066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171555.1
CDS	93..3731
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDI

Target ORF information:

RefSeq Version	XM_017316066.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316066.2

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_017316066.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171555.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu78793
Clone ID Related Accession (Same CDS sequence)	XM_017316066.2 , XM_017316066.1
Accession Version	XM_017316066.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171555.1
CDS	93..3731
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDI

Target ORF information:

RefSeq Version	XM_017316066.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316066.1

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_017316062.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171551.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X1, mRNA.

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CloneID	OMu74946
Clone ID Related Accession (Same CDS sequence)	XM_017316062.1 , XM_017316062.2
Accession Version	XM_017316062.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316062.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATTGCAT TGCTGGTTCT GGCCCAGTCT 
GTGTTGCTAT CTGTCAAGTG GGATGTTGAC GATCCTGTGA CGGTTCCTTT GGCAACAATG 
TCAGTTGTCT TCACCTTCAT CTTTGTCTTA GAGGTTACAA TGAAGATTAT AGCAATGTCA 
CCAGCTGGAT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCTTGGTGAC ATCTCTTGGT 
GTTGTGTGGG TGGTGCTCCA TTTTGCTCTG CTGAATGCAT ACACCTACAT GATGGGAGCC 
TGCGTGATTG TCTTTAGATT TTTCTCCATC TGTGGGAAGC ATGTGACATT AAAGATGCTC 
CTTCTGACTG TGGTTGTCAG CATGTACAAG AGCTTTTTTA TCATCGTAGG AATGTTTCTC 
TTGCTGCTGT GCTATGCCTT TGCTGGAGTT GTTCTGTTTG GTACCGTAAA GTATGGTGAG 
AACATTAACA GGCATGCCAA TTTTTCCTCA GCTGGCAAAG CCATTACTGT ATTGTTTCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGATT GTATGGTTCA ACCTCCATTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GCAATTATGC AGGGGCACTT 
ATGTACTTCT GCTCATTCTA TGTCATCATT GCCTACATCA TGCTGAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC TTTGTTTTAT TCCACTGAAG AGGACCAGCT TTTGAGTTAC 
AATGATCTTC GCCATTTTCA AATCATATGG AACATGGTAG ATGATAAAAG AGAGGGTGTG 
ATCCCCACTT TCCGAGTGAA GTTCCTGCTA CGGCTGCTGC GTGGGAGGCT GGAAGTGGAT 
CTTGATAAAG ACAAGCTCCT GTTTAAGCAT ATGTGCTATG AGATGGAGAG GCTGCACAAT 
GGTGGTGATG TCACCTTCCA TGATGTCTTA AGCATGCTCT CCTATCGCTC TGTAGACATC 
AGAAAAAGCC TGCAGCTAGA GGAGCTGCTG GCAAGGGAGC AGCTAGAATA CACCATAGAG 
GAGGAGGTGG CTAAGCAGAC TATTCGCATG TGGCTGAAGA AGTGCTTGAA ACGCATCCGG 
GCTAAACAAC AGCAGTCGTG CAGCATCATC CACAGCCTGA GAGAGAGCCA GGAGCAAGAG 
CGGAGCCGGC TATTCCTGAA TCCTCCCAGC ATTGAGACCA CCCAGCCAAG TGAGGACAGC 
AACGCCAACA GCCAGGACCA CAGTATGCAA CCTGAGACAA GCAGTCAGCA GCAGCTCTTA 
AGCCCTACTC TGTCAGACAG AGGAGGAAGC CGACAGGATG CAGCAGATAC TGGAAAACCC 
CAAAGGAAGA TTGGGCAATG GCGTCTGCCC TCAGCTGCCA TCGCTGAGAA GACGGCACCT 
GTTAACAAAA TGAGCGGTTA CAGCCAAGAT CCAGAAAGAG AGGAGGAAGA GGTTTCCAAG 
GGCTTCCTGG CCCCCAAACC AATAAGCCAT TCTGTATCTT CGGTTAACCT ACGGTTTGGA 
GGAAGGACAA CGATGAAGAC TGTGGTGTGC AAGATGAACC CCATGCCAGA CACAGCTTCC 
TGTGGCTCTG AAGTTAAAAA GTGGTGGACC AGACAGCTGA CCGTGGAGAG TGACGAGAGT 
GGAGATGACC TCCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171551.1
CDS	89..5452
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAAIAEKTAPVNKMSGYSQDPEREEEEVSKGFLAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_017316062.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316062.2

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATTGCAT TGCTGGTTCT GGCCCAGTCT 
GTGTTGCTAT CTGTCAAGTG GGATGTTGAC GATCCTGTGA CGGTTCCTTT GGCAACAATG 
TCAGTTGTCT TCACCTTCAT CTTTGTCTTA GAGGTTACAA TGAAGATTAT AGCAATGTCA 
CCAGCTGGAT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCTTGGTGAC ATCTCTTGGT 
GTTGTGTGGG TGGTGCTCCA TTTTGCTCTG CTGAATGCAT ACACCTACAT GATGGGAGCC 
TGCGTGATTG TCTTTAGATT TTTCTCCATC TGTGGGAAGC ATGTGACATT AAAGATGCTC 
CTTCTGACTG TGGTTGTCAG CATGTACAAG AGCTTTTTTA TCATCGTAGG AATGTTTCTC 
TTGCTGCTGT GCTATGCCTT TGCTGGAGTT GTTCTGTTTG GTACCGTAAA GTATGGTGAG 
AACATTAACA GGCATGCCAA TTTTTCCTCA GCTGGCAAAG CCATTACTGT ATTGTTTCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGATT GTATGGTTCA ACCTCCATTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GCAATTATGC AGGGGCACTT 
ATGTACTTCT GCTCATTCTA TGTCATCATT GCCTACATCA TGCTGAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC TTTGTTTTAT TCCACTGAAG AGGACCAGCT TTTGAGTTAC 
AATGATCTTC GCCATTTTCA AATCATATGG AACATGGTAG ATGATAAAAG AGAGGGTGTG 
ATCCCCACTT TCCGAGTGAA GTTCCTGCTA CGGCTGCTGC GTGGGAGGCT GGAAGTGGAT 
CTTGATAAAG ACAAGCTCCT GTTTAAGCAT ATGTGCTATG AGATGGAGAG GCTGCACAAT 
GGTGGTGATG TCACCTTCCA TGATGTCTTA AGCATGCTCT CCTATCGCTC TGTAGACATC 
AGAAAAAGCC TGCAGCTAGA GGAGCTGCTG GCAAGGGAGC AGCTAGAATA CACCATAGAG 
GAGGAGGTGG CTAAGCAGAC TATTCGCATG TGGCTGAAGA AGTGCTTGAA ACGCATCCGG 
GCTAAACAAC AGCAGTCGTG CAGCATCATC CACAGCCTGA GAGAGAGCCA GGAGCAAGAG 
CGGAGCCGGC TATTCCTGAA TCCTCCCAGC ATTGAGACCA CCCAGCCAAG TGAGGACAGC 
AACGCCAACA GCCAGGACCA CAGTATGCAA CCTGAGACAA GCAGTCAGCA GCAGCTCTTA 
AGCCCTACTC TGTCAGACAG AGGAGGAAGC CGACAGGATG CAGCAGATAC TGGAAAACCC 
CAAAGGAAGA TTGGGCAATG GCGTCTGCCC TCAGCTGCCA TCGCTGAGAA GACGGCACCT 
GTTAACAAAA TGAGCGGTTA CAGCCAAGAT CCAGAAAGAG AGGAGGAAGA GGTTTCCAAG 
GGCTTCCTGG CCCCCAAACC AATAAGCCAT TCTGTATCTT CGGTTAACCT ACGGTTTGGA 
GGAAGGACAA CGATGAAGAC TGTGGTGTGC AAGATGAACC CCATGCCAGA CACAGCTTCC 
TGTGGCTCTG AAGTTAAAAA GTGGTGGACC AGACAGCTGA CCGTGGAGAG TGACGAGAGT 
GGAGATGACC TCCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_011245094.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243396.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X2, mRNA.

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CloneID	OMu74947
Clone ID Related Accession (Same CDS sequence)	XM_011245094.3 , XM_011245094.2
Accession Version	XM_011245094.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GAAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTTACTGA CTTTGGTCCT 
GATGAATCTC TGTCAGACAA TGCTGACATA CTCTGGATTA ATAAGCCATG GGTGCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTGT GCATGAACAC ACCTATGACC 
TTTGAGCACT ATCCTCCTCT TCAGTATGTG ACCTTCACCT TGGACACTTT ATTGATGTTC 
CTCTACACTG CAGAAATGAT AGCAAAGATG CACATACGGG GAATTGTCAA GGGTGATAGC 
TCCTATGTGA AGGATCGCTG GTGTGTTTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCTCTTGTGT TACAGGTGTT TGAAATAGCT GACATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGCTGCGGA TCCCACGGCC ACTCATTATG ATCCGGGCTT TCAGGATTTA TTTCCGATTC 
GAACTGCCAA GGACAAGAAT TACAAACATT TTAAAGCGGT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTCCTTCTT CTGTATGGGA TTTTAGGAGT TCAAATGTTT 
GGAACATTTA CCTACCACTG TGTAGTCAAT GACACAAAGC CAGGAAATGT AACCTGGAAT 
AGCTTAGCTA TTCCAGATAC GCACTGCTCC CCAGAGCTAG AAGAAGGCTA TCAATGCCCG 
CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG CACGAGTATA TTCACAGTCT ATGAGGCTTC ATCTCAGGAA 
GGCTGGGTAT TCCTCATGTA CAGAGCAATC GACAGCTTTC CCCGCTGGCG CTCCTACTTC 
TACTTCATCA CACTGATTTT CTTCCTTGCT TGGCTTGTCA AGAATGTGTT TATTGCTGTC 
ATCATTGAGA CATTTGCAGA AATCAGAGTA CAATTTCAAC AAATGTGGGG AACTCGGAGC 
AGCACAACTT CTACAGCCAC CACACAGATG TTCCATGAAG ATGCCGCTGG TGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGTGCCC CAGCCTGCCT ACAGAAAATG 
ATGCGGTCAT CGGTTTTCCA CATGTTTATC CTGAGCATGG TGACTGTGGA TGTAATAGTT 
GCTGCCAGCA ATTACTACAA GGGAGAGAAC TTCAGAAGGC AGTATGATGA ATTCTACCTT 
GCAGAGGTGG CTTTTACAGT CCTTTTCGAC CTGGAAGCAC TCCTGAAGAT ATGGTGTTTG 
GGGTTTACTG GCTACATCAG CTCATCTCTC CACAAGTTTG AACTATTGCT TGTTATTGGG 
ACGACGCTTC ATGTGTACCC AGATCTTTAT CATTCTCAGT TCACATACTT CCAGGTTCTG 
CGGGTAGTCC GGCTTATTAA GATTTCCCCA GCATTGGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGGA AAAAACTTGG AAGCTTGGTG GTGTTCACTG CCAGCCTCCT GATAGTTATG 
TCAGCCATCA GTTTGCAGAT GTTCTGTTTT GTCGAAGAAC TGGACAGATT CACCACTTTT 
CCAAGGGCAT TTATGTCTAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGATGTGATG 
GATCAGACTC TGAATGCTGT GGGGCATATG TGGGCACCAC TGGTTGCCAT CTATTTCATC 
CTCTATCATC TCTTTGCAAC TTTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA TCTAAAGAAG CTCAAACAAC TAAAGCAAAG TGAAGCGAAC 
GCGGACACCA AAGAAAAACT CCCTTTGCGC TTGAGAATCT TCGAAAAATT TCCAAACAGA 
CCGCAAATGG TGAAAATCTC AAAACTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGCTTCATGA AGCAGTTCAT TGACCGCCAG CAACAGGACA CCTGCTGTCT CTTCAGAATC 
CTCCCCTCTA CCTCTTCCTC ATCATGTGAC AACCCCAAGA AGCCCACAGC TGAAGACAAC 
AAATACATTG ATCAAAAACT CCGCAAGTCT GTTTTCAGCA TAAGGGCGAG GAACCTACTG 
GAAAAGGAGA CTGCAGTCAC AAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC AGCAAAGGAG AGGTCGATCC TCAGCGTGCA GCATCACATC 
CGCCAGGAGC GCAGGTCACT AAGACATGGA TCCAACAGCC AGAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTTTAACTCA AGACCATTCA AATACAGTGC GCTACAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAAAT GATTCAGGAG AAGAAGGAAC AAGCCGAGAT GAAAAGGAAG 
GTGCAAGAAG AGGAACTGCG GGAGAACCAC CCATACTTTG ACAAGCCTCT CTTCATCGTG 
GGGCGGGAGC ACAGGTTCAG AAACTTCTGC CGCGTGGTGG TTCGAGCACG CTTCAATGCA 
TCCAAAACAG ACCCTGTCAC GGGAGCTGTG AAAAATACAA AGTACCACCA ACTTTATGAT 
TTGCTGGGAC TTGTCACCTA CCTGGACTGG GTCATGATCA CTGTAACCAT CTGCTCTTGC 
ATTTCTATGA TGTTCGAATC CCCCTTCCGA AGAGTCATGC ATGCACCCAC TTTACAGATC 
GCAGAATATG TGTTTGTAAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTGTTTT TCACTCCAAC TGCTGTCATC AGGGACTTTG GTGGCGTCAT GGACATATTT 
ATCTATCTTG TGAGTTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCCGAGTCA 
GGAGCCCAGC TACTGATGGT TCTTCGGTGC CTAAGACCTC TTCGGATATT CAAACTTGTG 
CCCCAAATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGTT TCAAGGAAAT ATTTTTGGTC 
TCCATTCTGT TGTTGACGTT AATGCTTGTT TTTGCAAGCT TTGGGGTTCA GCTCTTTGCT 
GGAAAGTTAG CCAAGTGCAA CGACCCCAAC ATTATCAGAA GGGAAGACTG TAATGGCATC 
TTCAGAATTA ATGTAAGTGT GTCCAAGAAC TTAAATTTAA AACTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTCGACAAT 
GTGGGAAATG CCATGCTGGC ATTATTTGAA GTTCTGTCCT TGAAAGGCTG GGTAGAAGTG 
AGGGATGTCA TTATTCATCG TGTGGGGCCG ATCCACGGAA TCTATATTCA TGTTTTCGTA 
TTCCTGGGTT GCATGATCGG ACTGACGCTT TTTGTCGGTG TAGTTATTGC TAACTTCAAT 
GAAAACAAGG GGACAGCCCT GCTGACGGTA GATCAGAGAC GATGGGAAGA TCTCAAGAGC 
AGACTGAAGA TCGCACAGCC TCTTCATCTC CCTCCTCGGC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATTGC ATTGCTGGTT 
CTGGCCCAGT CTGTGTTGCT ATCTGTCAAG TGGGATGTTG ACGATCCTGT GACGGTTCCT 
TTGGCAACAA TGTCAGTTGT CTTCACCTTC ATCTTTGTCT TAGAGGTTAC AATGAAGATT 
ATAGCAATGT CACCAGCTGG ATTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCTTGGTG 
ACATCTCTTG GTGTTGTGTG GGTGGTGCTC CATTTTGCTC TGCTGAATGC ATACACCTAC 
ATGATGGGAG CCTGCGTGAT TGTCTTTAGA TTTTTCTCCA TCTGTGGGAA GCATGTGACA 
TTAAAGATGC TCCTTCTGAC TGTGGTTGTC AGCATGTACA AGAGCTTTTT TATCATCGTA 
GGAATGTTTC TCTTGCTGCT GTGCTATGCC TTTGCTGGAG TTGTTCTGTT TGGTACCGTA 
AAGTATGGTG AGAACATTAA CAGGCATGCC AATTTTTCCT CAGCTGGCAA AGCCATTACT 
GTATTGTTTC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA TTGTATGGTT 
CAACCTCCAT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGCAATTAT 
GCAGGGGCAC TTATGTACTT CTGCTCATTC TATGTCATCA TTGCCTACAT CATGCTGAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCTTTGTTTT ATTCCACTGA AGAGGACCAG 
CTTTTGAGTT ACAATGATCT TCGCCATTTT CAAATCATAT GGAACATGGT AGATGATAAA 
AGAGAGGGTG TGATCCCCAC TTTCCGAGTG AAGTTCCTGC TACGGCTGCT GCGTGGGAGG 
CTGGAAGTGG ATCTTGATAA AGACAAGCTC CTGTTTAAGC ATATGTGCTA TGAGATGGAG 
AGGCTGCACA ATGGTGGTGA TGTCACCTTC CATGATGTCT TAAGCATGCT CTCCTATCGC 
TCTGTAGACA TCAGAAAAAG CCTGCAGCTA GAGGAGCTGC TGGCAAGGGA GCAGCTAGAA 
TACACCATAG AGGAGGAGGT GGCTAAGCAG ACTATTCGCA TGTGGCTGAA GAAGTGCTTG 
AAACGCATCC GGGCTAAACA ACAGCAGTCG TGCAGCATCA TCCACAGCCT GAGAGAGAGC 
CAGGAGCAAG AGCGGAGCCG GCTATTCCTG AATCCTCCCA GCATTGAGAC CACCCAGCCA 
AGTGAGGACA GCAACGCCAA CAGCCAGGAC CACAGTATGC AACCTGAGAC AAGCAGTCAG 
CAGCAGCTCT TAAGCCCTAC TCTGTCAGAC AGAGGAGGAA GCCGACAGGA TGCAGCAGAT 
ACTGGAAAAC CCCAAAGGAA GATTGGGCAA TGGCGTCTGC CCTCAGCTGC CATCGCTGAG 
AAGACGGCAC CTGTTAACAA AATGAGCGGT TACAGCCAAG ATCCAGAAAG AGAGGAGGAA 
GAGGTTTCCA AGGGCTTCCT GGCCCCCAAA CCAATAAGCC ATTCTGTATC TTCGGTTAAC 
CTACGGTTTG GAGGAAGGAC AACGATGAAG ACTGTGGTGT GCAAGATGAA CCCCATGCCA 
GACACAGCTT CCTGTGGCTC TGAAGTTAAA AAGTGGTGGA CCAGACAGCT GACCGTGGAG 
AGTGACGAGA GTGGAGATGA CCTCCTGGAT ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243396.1
CDS	555..5870
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAAIAEKTAPVNKMSGYSQDPEREEEEVSKGFLAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_011245094.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245094.3

ORF Insert Sequence:

ATGCTCAAAA GAAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTTACTGA CTTTGGTCCT 
GATGAATCTC TGTCAGACAA TGCTGACATA CTCTGGATTA ATAAGCCATG GGTGCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTGT GCATGAACAC ACCTATGACC 
TTTGAGCACT ATCCTCCTCT TCAGTATGTG ACCTTCACCT TGGACACTTT ATTGATGTTC 
CTCTACACTG CAGAAATGAT AGCAAAGATG CACATACGGG GAATTGTCAA GGGTGATAGC 
TCCTATGTGA AGGATCGCTG GTGTGTTTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCTCTTGTGT TACAGGTGTT TGAAATAGCT GACATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGCTGCGGA TCCCACGGCC ACTCATTATG ATCCGGGCTT TCAGGATTTA TTTCCGATTC 
GAACTGCCAA GGACAAGAAT TACAAACATT TTAAAGCGGT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTCCTTCTT CTGTATGGGA TTTTAGGAGT TCAAATGTTT 
GGAACATTTA CCTACCACTG TGTAGTCAAT GACACAAAGC CAGGAAATGT AACCTGGAAT 
AGCTTAGCTA TTCCAGATAC GCACTGCTCC CCAGAGCTAG AAGAAGGCTA TCAATGCCCG 
CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG CACGAGTATA TTCACAGTCT ATGAGGCTTC ATCTCAGGAA 
GGCTGGGTAT TCCTCATGTA CAGAGCAATC GACAGCTTTC CCCGCTGGCG CTCCTACTTC 
TACTTCATCA CACTGATTTT CTTCCTTGCT TGGCTTGTCA AGAATGTGTT TATTGCTGTC 
ATCATTGAGA CATTTGCAGA AATCAGAGTA CAATTTCAAC AAATGTGGGG AACTCGGAGC 
AGCACAACTT CTACAGCCAC CACACAGATG TTCCATGAAG ATGCCGCTGG TGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGTGCCC CAGCCTGCCT ACAGAAAATG 
ATGCGGTCAT CGGTTTTCCA CATGTTTATC CTGAGCATGG TGACTGTGGA TGTAATAGTT 
GCTGCCAGCA ATTACTACAA GGGAGAGAAC TTCAGAAGGC AGTATGATGA ATTCTACCTT 
GCAGAGGTGG CTTTTACAGT CCTTTTCGAC CTGGAAGCAC TCCTGAAGAT ATGGTGTTTG 
GGGTTTACTG GCTACATCAG CTCATCTCTC CACAAGTTTG AACTATTGCT TGTTATTGGG 
ACGACGCTTC ATGTGTACCC AGATCTTTAT CATTCTCAGT TCACATACTT CCAGGTTCTG 
CGGGTAGTCC GGCTTATTAA GATTTCCCCA GCATTGGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGGA AAAAACTTGG AAGCTTGGTG GTGTTCACTG CCAGCCTCCT GATAGTTATG 
TCAGCCATCA GTTTGCAGAT GTTCTGTTTT GTCGAAGAAC TGGACAGATT CACCACTTTT 
CCAAGGGCAT TTATGTCTAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGATGTGATG 
GATCAGACTC TGAATGCTGT GGGGCATATG TGGGCACCAC TGGTTGCCAT CTATTTCATC 
CTCTATCATC TCTTTGCAAC TTTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA TCTAAAGAAG CTCAAACAAC TAAAGCAAAG TGAAGCGAAC 
GCGGACACCA AAGAAAAACT CCCTTTGCGC TTGAGAATCT TCGAAAAATT TCCAAACAGA 
CCGCAAATGG TGAAAATCTC AAAACTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGCTTCATGA AGCAGTTCAT TGACCGCCAG CAACAGGACA CCTGCTGTCT CTTCAGAATC 
CTCCCCTCTA CCTCTTCCTC ATCATGTGAC AACCCCAAGA AGCCCACAGC TGAAGACAAC 
AAATACATTG ATCAAAAACT CCGCAAGTCT GTTTTCAGCA TAAGGGCGAG GAACCTACTG 
GAAAAGGAGA CTGCAGTCAC AAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC AGCAAAGGAG AGGTCGATCC TCAGCGTGCA GCATCACATC 
CGCCAGGAGC GCAGGTCACT AAGACATGGA TCCAACAGCC AGAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTTTAACTCA AGACCATTCA AATACAGTGC GCTACAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAAAT GATTCAGGAG AAGAAGGAAC AAGCCGAGAT GAAAAGGAAG 
GTGCAAGAAG AGGAACTGCG GGAGAACCAC CCATACTTTG ACAAGCCTCT CTTCATCGTG 
GGGCGGGAGC ACAGGTTCAG AAACTTCTGC CGCGTGGTGG TTCGAGCACG CTTCAATGCA 
TCCAAAACAG ACCCTGTCAC GGGAGCTGTG AAAAATACAA AGTACCACCA ACTTTATGAT 
TTGCTGGGAC TTGTCACCTA CCTGGACTGG GTCATGATCA CTGTAACCAT CTGCTCTTGC 
ATTTCTATGA TGTTCGAATC CCCCTTCCGA AGAGTCATGC ATGCACCCAC TTTACAGATC 
GCAGAATATG TGTTTGTAAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTGTTTT TCACTCCAAC TGCTGTCATC AGGGACTTTG GTGGCGTCAT GGACATATTT 
ATCTATCTTG TGAGTTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCCGAGTCA 
GGAGCCCAGC TACTGATGGT TCTTCGGTGC CTAAGACCTC TTCGGATATT CAAACTTGTG 
CCCCAAATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGTT TCAAGGAAAT ATTTTTGGTC 
TCCATTCTGT TGTTGACGTT AATGCTTGTT TTTGCAAGCT TTGGGGTTCA GCTCTTTGCT 
GGAAAGTTAG CCAAGTGCAA CGACCCCAAC ATTATCAGAA GGGAAGACTG TAATGGCATC 
TTCAGAATTA ATGTAAGTGT GTCCAAGAAC TTAAATTTAA AACTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTCGACAAT 
GTGGGAAATG CCATGCTGGC ATTATTTGAA GTTCTGTCCT TGAAAGGCTG GGTAGAAGTG 
AGGGATGTCA TTATTCATCG TGTGGGGCCG ATCCACGGAA TCTATATTCA TGTTTTCGTA 
TTCCTGGGTT GCATGATCGG ACTGACGCTT TTTGTCGGTG TAGTTATTGC TAACTTCAAT 
GAAAACAAGG GGACAGCCCT GCTGACGGTA GATCAGAGAC GATGGGAAGA TCTCAAGAGC 
AGACTGAAGA TCGCACAGCC TCTTCATCTC CCTCCTCGGC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATTGC ATTGCTGGTT 
CTGGCCCAGT CTGTGTTGCT ATCTGTCAAG TGGGATGTTG ACGATCCTGT GACGGTTCCT 
TTGGCAACAA TGTCAGTTGT CTTCACCTTC ATCTTTGTCT TAGAGGTTAC AATGAAGATT 
ATAGCAATGT CACCAGCTGG ATTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCTTGGTG 
ACATCTCTTG GTGTTGTGTG GGTGGTGCTC CATTTTGCTC TGCTGAATGC ATACACCTAC 
ATGATGGGAG CCTGCGTGAT TGTCTTTAGA TTTTTCTCCA TCTGTGGGAA GCATGTGACA 
TTAAAGATGC TCCTTCTGAC TGTGGTTGTC AGCATGTACA AGAGCTTTTT TATCATCGTA 
GGAATGTTTC TCTTGCTGCT GTGCTATGCC TTTGCTGGAG TTGTTCTGTT TGGTACCGTA 
AAGTATGGTG AGAACATTAA CAGGCATGCC AATTTTTCCT CAGCTGGCAA AGCCATTACT 
GTATTGTTTC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA TTGTATGGTT 
CAACCTCCAT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGCAATTAT 
GCAGGGGCAC TTATGTACTT CTGCTCATTC TATGTCATCA TTGCCTACAT CATGCTGAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCTTTGTTTT ATTCCACTGA AGAGGACCAG 
CTTTTGAGTT ACAATGATCT TCGCCATTTT CAAATCATAT GGAACATGGT AGATGATAAA 
AGAGAGGGTG TGATCCCCAC TTTCCGAGTG AAGTTCCTGC TACGGCTGCT GCGTGGGAGG 
CTGGAAGTGG ATCTTGATAA AGACAAGCTC CTGTTTAAGC ATATGTGCTA TGAGATGGAG 
AGGCTGCACA ATGGTGGTGA TGTCACCTTC CATGATGTCT TAAGCATGCT CTCCTATCGC 
TCTGTAGACA TCAGAAAAAG CCTGCAGCTA GAGGAGCTGC TGGCAAGGGA GCAGCTAGAA 
TACACCATAG AGGAGGAGGT GGCTAAGCAG ACTATTCGCA TGTGGCTGAA GAAGTGCTTG 
AAACGCATCC GGGCTAAACA ACAGCAGTCG TGCAGCATCA TCCACAGCCT GAGAGAGAGC 
CAGGAGCAAG AGCGGAGCCG GCTATTCCTG AATCCTCCCA GCATTGAGAC CACCCAGCCA 
AGTGAGGACA GCAACGCCAA CAGCCAGGAC CACAGTATGC AACCTGAGAC AAGCAGTCAG 
CAGCAGCTCT TAAGCCCTAC TCTGTCAGAC AGAGGAGGAA GCCGACAGGA TGCAGCAGAT 
ACTGGAAAAC CCCAAAGGAA GATTGGGCAA TGGCGTCTGC CCTCAGCTGC CATCGCTGAG 
AAGACGGCAC CTGTTAACAA AATGAGCGGT TACAGCCAAG ATCCAGAAAG AGAGGAGGAA 
GAGGTTTCCA AGGGCTTCCT GGCCCCCAAA CCAATAAGCC ATTCTGTATC TTCGGTTAAC 
CTACGGTTTG GAGGAAGGAC AACGATGAAG ACTGTGGTGT GCAAGATGAA CCCCATGCCA 
GACACAGCTT CCTGTGGCTC TGAAGTTAAA AAGTGGTGGA CCAGACAGCT GACCGTGGAG 
AGTGACGAGA GTGGAGATGA CCTCCTGGAT ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_017316063.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171552.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X3, mRNA.

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CloneID	OMu74948
Clone ID Related Accession (Same CDS sequence)	XM_017316063.1 , XM_017316063.2
Accession Version	XM_017316063.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGCTAAAGCA AAGTGAAGCG AACGCGGACA CCAAAGAAAA ACTCCCTTTG 
CGCTTGAGAA TCTTCGAAAA ATTTCCAAAC AGACCGCAAA TGGTGAAAAT CTCAAAACTT 
CCTTCAGATT TTACAGTTCC TAAAATCAGG GAAAGCTTCA TGAAGCAGTT CATTGACCGC 
CAGCAACAGG ACACCTGCTG TCTCTTCAGA ATCCTCCCCT CTACCTCTTC CTCATCATGT 
GACAACCCCA AGAAGCCCAC AGCTGAAGAC AACAAATACA TTGATCAAAA ACTCCGCAAG 
TCTGTTTTCA GCATAAGGGC GAGGAACCTA CTGGAAAAGG AGACTGCAGT CACAAAAATC 
TTAAGAGCTT GCACTCGACA GCGCATGCTG AGCGGATCAT TCGAGGGGCA GCCAGCAAAG 
GAGAGGTCGA TCCTCAGCGT GCAGCATCAC ATCCGCCAGG AGCGCAGGTC ACTAAGACAT 
GGATCCAACA GCCAGAGGAT CAGCAGGGGG AAATCTCTTG AAACTTTAAC TCAAGACCAT 
TCAAATACAG TGCGCTACAG AAATGCACAA AGAGAAGACA GTGAAATAAA AATGATTCAG 
GAGAAGAAGG AACAAGCCGA GATGAAAAGG AAGGTGCAAG AAGAGGAACT GCGGGAGAAC 
CACCCATACT TTGACAAGCC TCTCTTCATC GTGGGGCGGG AGCACAGGTT CAGAAACTTC 
TGCCGCGTGG TGGTTCGAGC ACGCTTCAAT GCATCCAAAA CAGACCCTGT CACGGGAGCT 
GTGAAAAATA CAAAGTACCA CCAACTTTAT GATTTGCTGG GACTTGTCAC CTACCTGGAC 
TGGGTCATGA TCACTGTAAC CATCTGCTCT TGCATTTCTA TGATGTTCGA ATCCCCCTTC 
CGAAGAGTCA TGCATGCACC CACTTTACAG ATCGCAGAAT ATGTGTTTGT AATATTCATG 
AGCATTGAGC TTAATCTGAA GATTATGGCA GATGGCTTGT TTTTCACTCC AACTGCTGTC 
ATCAGGGACT TTGGTGGCGT CATGGACATA TTTATCTATC TTGTGAGTTT GATATTTCTT 
TGTTGGATGC CTCAAAATGT ACCTGCCGAG TCAGGAGCCC AGCTACTGAT GGTTCTTCGG 
TGCCTAAGAC CTCTTCGGAT ATTCAAACTT GTGCCCCAAA TGAGGAAAGT TGTTCGAGAA 
CTTTTCAGTG GTTTCAAGGA AATATTTTTG GTCTCCATTC TGTTGTTGAC GTTAATGCTT 
GTTTTTGCAA GCTTTGGGGT TCAGCTCTTT GCTGGAAAGT TAGCCAAGTG CAACGACCCC 
AACATTATCA GAAGGGAAGA CTGTAATGGC ATCTTCAGAA TTAATGTAAG TGTGTCCAAG 
AACTTAAATT TAAAACTAAG ACCTGGAGAG AAAAAACCTG GATTTTGGGT GCCCCGTGTT 
TGGGCAAATC CTCGGAACTT TAACTTCGAC AATGTGGGAA ATGCCATGCT GGCATTATTT 
GAAGTTCTGT CCTTGAAAGG CTGGGTAGAA GTGAGGGATG TCATTATTCA TCGTGTGGGG 
CCGATCCACG GAATCTATAT TCATGTTTTC GTATTCCTGG GTTGCATGAT CGGACTGACG 
CTTTTTGTCG GTGTAGTTAT TGCTAACTTC AATGAAAACA AGGGGACAGC CCTGCTGACG 
GTAGATCAGA GACGATGGGA AGATCTCAAG AGCAGACTGA AGATCGCACA GCCTCTTCAT 
CTCCCTCCTC GGCCGGATAA TGATGGTTTT AGAGCTAAAA TGTATGACAT AACCCAGCAT 
CCATTTTTTA AGAGGACAAT TGCATTGCTG GTTCTGGCCC AGTCTGTGTT GCTATCTGTC 
AAGTGGGATG TTGACGATCC TGTGACGGTT CCTTTGGCAA CAATGTCAGT TGTCTTCACC 
TTCATCTTTG TCTTAGAGGT TACAATGAAG ATTATAGCAA TGTCACCAGC TGGATTCTGG 
CAAAGCAGAA GAAACCGATA TGATCTCTTG GTGACATCTC TTGGTGTTGT GTGGGTGGTG 
CTCCATTTTG CTCTGCTGAA TGCATACACC TACATGATGG GAGCCTGCGT GATTGTCTTT 
AGATTTTTCT CCATCTGTGG GAAGCATGTG ACATTAAAGA TGCTCCTTCT GACTGTGGTT 
GTCAGCATGT ACAAGAGCTT TTTTATCATC GTAGGAATGT TTCTCTTGCT GCTGTGCTAT 
GCCTTTGCTG GAGTTGTTCT GTTTGGTACC GTAAAGTATG GTGAGAACAT TAACAGGCAT 
GCCAATTTTT CCTCAGCTGG CAAAGCCATT ACTGTATTGT TTCGAATTGT CACAGGTGAA 
GACTGGAACA AGATTATGCA TGATTGTATG GTTCAACCTC CATTTTGTAC TCCAGATGAA 
TTTACATACT GGGCAACAGA CTGTGGCAAT TATGCAGGGG CACTTATGTA CTTCTGCTCA 
TTCTATGTCA TCATTGCCTA CATCATGCTG AATCTGCTTG TAGCCATAAT TGTGGAGAAT 
TTCTCTTTGT TTTATTCCAC TGAAGAGGAC CAGCTTTTGA GTTACAATGA TCTTCGCCAT 
TTTCAAATCA TATGGAACAT GGTAGATGAT AAAAGAGAGG GTGTGATCCC CACTTTCCGA 
GTGAAGTTCC TGCTACGGCT GCTGCGTGGG AGGCTGGAAG TGGATCTTGA TAAAGACAAG 
CTCCTGTTTA AGCATATGTG CTATGAGATG GAGAGGCTGC ACAATGGTGG TGATGTCACC 
TTCCATGATG TCTTAAGCAT GCTCTCCTAT CGCTCTGTAG ACATCAGAAA AAGCCTGCAG 
CTAGAGGAGC TGCTGGCAAG GGAGCAGCTA GAATACACCA TAGAGGAGGA GGTGGCTAAG 
CAGACTATTC GCATGTGGCT GAAGAAGTGC TTGAAACGCA TCCGGGCTAA ACAACAGCAG 
TCGTGCAGCA TCATCCACAG CCTGAGAGAG AGCCAGGAGC AAGAGCGGAG CCGGCTATTC 
CTGAATCCTC CCAGCATTGA GACCACCCAG CCAAGTGAGG ACAGCAACGC CAACAGCCAG 
GACCACAGTA TGCAACCTGA GACAAGCAGT CAGCAGCAGC TCTTAAGCCC TACTCTGTCA 
GACAGAGGAG GAAGCCGACA GGATGCAGCA GATACTGGAA AACCCCAAAG GAAGATTGGG 
CAATGGCGTC TGCCCTCAGC TGCCATCGCT GAGAAGACGG CACCTGTTAA CAAAATGAGC 
GGTTACAGCC AAGATCCAGA AAGAGAGGAG GAAGAGGTTT CCAAGGGCTT CCTGGCCCCC 
AAACCAATAA GCCATTCTGT ATCTTCGGTT AACCTACGGT TTGGAGGAAG GACAACGATG 
AAGACTGTGG TGTGCAAGAT GAACCCCATG CCAGACACAG CTTCCTGTGG CTCTGAAGTT 
AAAAAGTGGT GGACCAGACA GCTGACCGTG GAGAGTGACG AGAGTGGAGA TGACCTCCTG 
GATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171552.1
CDS	89..5377
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAAIAEKTAPVNKMSGYSQDPEREEEEVSKGFLAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_017316063.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316063.2

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGCTAAAGCA AAGTGAAGCG AACGCGGACA CCAAAGAAAA ACTCCCTTTG 
CGCTTGAGAA TCTTCGAAAA ATTTCCAAAC AGACCGCAAA TGGTGAAAAT CTCAAAACTT 
CCTTCAGATT TTACAGTTCC TAAAATCAGG GAAAGCTTCA TGAAGCAGTT CATTGACCGC 
CAGCAACAGG ACACCTGCTG TCTCTTCAGA ATCCTCCCCT CTACCTCTTC CTCATCATGT 
GACAACCCCA AGAAGCCCAC AGCTGAAGAC AACAAATACA TTGATCAAAA ACTCCGCAAG 
TCTGTTTTCA GCATAAGGGC GAGGAACCTA CTGGAAAAGG AGACTGCAGT CACAAAAATC 
TTAAGAGCTT GCACTCGACA GCGCATGCTG AGCGGATCAT TCGAGGGGCA GCCAGCAAAG 
GAGAGGTCGA TCCTCAGCGT GCAGCATCAC ATCCGCCAGG AGCGCAGGTC ACTAAGACAT 
GGATCCAACA GCCAGAGGAT CAGCAGGGGG AAATCTCTTG AAACTTTAAC TCAAGACCAT 
TCAAATACAG TGCGCTACAG AAATGCACAA AGAGAAGACA GTGAAATAAA AATGATTCAG 
GAGAAGAAGG AACAAGCCGA GATGAAAAGG AAGGTGCAAG AAGAGGAACT GCGGGAGAAC 
CACCCATACT TTGACAAGCC TCTCTTCATC GTGGGGCGGG AGCACAGGTT CAGAAACTTC 
TGCCGCGTGG TGGTTCGAGC ACGCTTCAAT GCATCCAAAA CAGACCCTGT CACGGGAGCT 
GTGAAAAATA CAAAGTACCA CCAACTTTAT GATTTGCTGG GACTTGTCAC CTACCTGGAC 
TGGGTCATGA TCACTGTAAC CATCTGCTCT TGCATTTCTA TGATGTTCGA ATCCCCCTTC 
CGAAGAGTCA TGCATGCACC CACTTTACAG ATCGCAGAAT ATGTGTTTGT AATATTCATG 
AGCATTGAGC TTAATCTGAA GATTATGGCA GATGGCTTGT TTTTCACTCC AACTGCTGTC 
ATCAGGGACT TTGGTGGCGT CATGGACATA TTTATCTATC TTGTGAGTTT GATATTTCTT 
TGTTGGATGC CTCAAAATGT ACCTGCCGAG TCAGGAGCCC AGCTACTGAT GGTTCTTCGG 
TGCCTAAGAC CTCTTCGGAT ATTCAAACTT GTGCCCCAAA TGAGGAAAGT TGTTCGAGAA 
CTTTTCAGTG GTTTCAAGGA AATATTTTTG GTCTCCATTC TGTTGTTGAC GTTAATGCTT 
GTTTTTGCAA GCTTTGGGGT TCAGCTCTTT GCTGGAAAGT TAGCCAAGTG CAACGACCCC 
AACATTATCA GAAGGGAAGA CTGTAATGGC ATCTTCAGAA TTAATGTAAG TGTGTCCAAG 
AACTTAAATT TAAAACTAAG ACCTGGAGAG AAAAAACCTG GATTTTGGGT GCCCCGTGTT 
TGGGCAAATC CTCGGAACTT TAACTTCGAC AATGTGGGAA ATGCCATGCT GGCATTATTT 
GAAGTTCTGT CCTTGAAAGG CTGGGTAGAA GTGAGGGATG TCATTATTCA TCGTGTGGGG 
CCGATCCACG GAATCTATAT TCATGTTTTC GTATTCCTGG GTTGCATGAT CGGACTGACG 
CTTTTTGTCG GTGTAGTTAT TGCTAACTTC AATGAAAACA AGGGGACAGC CCTGCTGACG 
GTAGATCAGA GACGATGGGA AGATCTCAAG AGCAGACTGA AGATCGCACA GCCTCTTCAT 
CTCCCTCCTC GGCCGGATAA TGATGGTTTT AGAGCTAAAA TGTATGACAT AACCCAGCAT 
CCATTTTTTA AGAGGACAAT TGCATTGCTG GTTCTGGCCC AGTCTGTGTT GCTATCTGTC 
AAGTGGGATG TTGACGATCC TGTGACGGTT CCTTTGGCAA CAATGTCAGT TGTCTTCACC 
TTCATCTTTG TCTTAGAGGT TACAATGAAG ATTATAGCAA TGTCACCAGC TGGATTCTGG 
CAAAGCAGAA GAAACCGATA TGATCTCTTG GTGACATCTC TTGGTGTTGT GTGGGTGGTG 
CTCCATTTTG CTCTGCTGAA TGCATACACC TACATGATGG GAGCCTGCGT GATTGTCTTT 
AGATTTTTCT CCATCTGTGG GAAGCATGTG ACATTAAAGA TGCTCCTTCT GACTGTGGTT 
GTCAGCATGT ACAAGAGCTT TTTTATCATC GTAGGAATGT TTCTCTTGCT GCTGTGCTAT 
GCCTTTGCTG GAGTTGTTCT GTTTGGTACC GTAAAGTATG GTGAGAACAT TAACAGGCAT 
GCCAATTTTT CCTCAGCTGG CAAAGCCATT ACTGTATTGT TTCGAATTGT CACAGGTGAA 
GACTGGAACA AGATTATGCA TGATTGTATG GTTCAACCTC CATTTTGTAC TCCAGATGAA 
TTTACATACT GGGCAACAGA CTGTGGCAAT TATGCAGGGG CACTTATGTA CTTCTGCTCA 
TTCTATGTCA TCATTGCCTA CATCATGCTG AATCTGCTTG TAGCCATAAT TGTGGAGAAT 
TTCTCTTTGT TTTATTCCAC TGAAGAGGAC CAGCTTTTGA GTTACAATGA TCTTCGCCAT 
TTTCAAATCA TATGGAACAT GGTAGATGAT AAAAGAGAGG GTGTGATCCC CACTTTCCGA 
GTGAAGTTCC TGCTACGGCT GCTGCGTGGG AGGCTGGAAG TGGATCTTGA TAAAGACAAG 
CTCCTGTTTA AGCATATGTG CTATGAGATG GAGAGGCTGC ACAATGGTGG TGATGTCACC 
TTCCATGATG TCTTAAGCAT GCTCTCCTAT CGCTCTGTAG ACATCAGAAA AAGCCTGCAG 
CTAGAGGAGC TGCTGGCAAG GGAGCAGCTA GAATACACCA TAGAGGAGGA GGTGGCTAAG 
CAGACTATTC GCATGTGGCT GAAGAAGTGC TTGAAACGCA TCCGGGCTAA ACAACAGCAG 
TCGTGCAGCA TCATCCACAG CCTGAGAGAG AGCCAGGAGC AAGAGCGGAG CCGGCTATTC 
CTGAATCCTC CCAGCATTGA GACCACCCAG CCAAGTGAGG ACAGCAACGC CAACAGCCAG 
GACCACAGTA TGCAACCTGA GACAAGCAGT CAGCAGCAGC TCTTAAGCCC TACTCTGTCA 
GACAGAGGAG GAAGCCGACA GGATGCAGCA GATACTGGAA AACCCCAAAG GAAGATTGGG 
CAATGGCGTC TGCCCTCAGC TGCCATCGCT GAGAAGACGG CACCTGTTAA CAAAATGAGC 
GGTTACAGCC AAGATCCAGA AAGAGAGGAG GAAGAGGTTT CCAAGGGCTT CCTGGCCCCC 
AAACCAATAA GCCATTCTGT ATCTTCGGTT AACCTACGGT TTGGAGGAAG GACAACGATG 
AAGACTGTGG TGTGCAAGAT GAACCCCATG CCAGACACAG CTTCCTGTGG CTCTGAAGTT 
AAAAAGTGGT GGACCAGACA GCTGACCGTG GAGAGTGACG AGAGTGGAGA TGACCTCCTG 
GATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_017316064.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171553.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X4, mRNA.

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CloneID	OMu74949
Clone ID Related Accession (Same CDS sequence)	XM_017316064.1 , XM_017316064.2
Accession Version	XM_017316064.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316064.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATTGCAT TGCTGGTTCT GGCCCAGTCT 
GTGTTGCTAT CTGTCAAGTG GGATGTTGAC GATCCTGTGA CGGTTCCTTT GGCAACAATG 
TCAGTTGTCT TCACCTTCAT CTTTGTCTTA GAGGTTACAA TGAAGATTAT AGCAATGTCA 
CCAGCTGGAT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCTTGGTGAC ATCTCTTGGT 
GTTGTGTGGG TGGTGCTCCA TTTTGCTCTG CTGAATGCAT ACACCTACAT GATGGGAGCC 
TGCGTGATTG TCTTTAGATT TTTCTCCATC TGTGGGAAGC ATGTGACATT AAAGATGCTC 
CTTCTGACTG TGGTTGTCAG CATGTACAAG AGCTTTTTTA TCATCGTAGG AATGTTTCTC 
TTGCTGCTGT GCTATGCCTT TGCTGGAGTT GTTCTGTTTG GTACCGTAAA GTATGGTGAG 
AACATTAACA GGCATGCCAA TTTTTCCTCA GCTGGCAAAG CCATTACTGT ATTGTTTCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGATT GTATGGTTCA ACCTCCATTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GCAATTATGC AGGGGCACTT 
ATGTACTTCT GCTCATTCTA TGTCATCATT GCCTACATCA TGCTGAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC TTTGTTTTAT TCCACTGAAG AGGACCAGCT TTTGAGTTAC 
AATGATCTTC GCCATTTTCA AATCATATGG AACATGGTAG ATGATAAAAG AGAGGGTGTG 
ATCCCCACTT TCCGAGTGAA GTTCCTGCTA CGGCTGCTGC GTGGGAGGCT GGAAGTGGAT 
CTTGATAAAG ACAAGCTCCT GTTTAAGCAT ATGTGCTATG AGATGGAGAG GCTGCACAAT 
GGTGGTGATG TCACCTTCCA TGATGTCTTA AGCATGCTCT CCTATCGCTC TGTAGACATC 
AGAAAAAGCC TGCAGCTAGA GGAGCTGCTG GCAAGGGAGC AGCTAGAATA CACCATAGAG 
GAGGAGGTGG CTAAGCAGAC TATTCGCATG TGGCTGAAGA AGTGCTTGAA ACGCATCCGG 
GCTAAACAAC AGCAGTCGTG CAGCATCATC CACAGCCTGA GAGAGAGCCA GGAGCAAGAG 
CGGAGCCGGC TATTCCTGAA TCCTCCCAGC ATTGAGACCA CCCAGCCAAG TGAGGACAGC 
AACGCCAACA GCCAGGACCA CAGTATGCAA CCTGAGACAA GCAGTCAGCA GCAGCTCTTA 
AGCCCTACTC TGTCAGACAG AGGAGGAAGC CGACAGGATG CAGCAGATAC TGGAAAACCC 
CAAAGGAAGA TTGGGCAATG GCGTCTGCCC TCAGCCCCCA AACCAATAAG CCATTCTGTA 
TCTTCGGTTA ACCTACGGTT TGGAGGAAGG ACAACGATGA AGACTGTGGT GTGCAAGATG 
AACCCCATGC CAGACACAGC TTCCTGTGGC TCTGAAGTTA AAAAGTGGTG GACCAGACAG 
CTGACCGTGG AGAGTGACGA GAGTGGAGAT GACCTCCTGG ATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171553.1
CDS	89..5356
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_017316064.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316064.2

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATTGCAT TGCTGGTTCT GGCCCAGTCT 
GTGTTGCTAT CTGTCAAGTG GGATGTTGAC GATCCTGTGA CGGTTCCTTT GGCAACAATG 
TCAGTTGTCT TCACCTTCAT CTTTGTCTTA GAGGTTACAA TGAAGATTAT AGCAATGTCA 
CCAGCTGGAT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCTTGGTGAC ATCTCTTGGT 
GTTGTGTGGG TGGTGCTCCA TTTTGCTCTG CTGAATGCAT ACACCTACAT GATGGGAGCC 
TGCGTGATTG TCTTTAGATT TTTCTCCATC TGTGGGAAGC ATGTGACATT AAAGATGCTC 
CTTCTGACTG TGGTTGTCAG CATGTACAAG AGCTTTTTTA TCATCGTAGG AATGTTTCTC 
TTGCTGCTGT GCTATGCCTT TGCTGGAGTT GTTCTGTTTG GTACCGTAAA GTATGGTGAG 
AACATTAACA GGCATGCCAA TTTTTCCTCA GCTGGCAAAG CCATTACTGT ATTGTTTCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGATT GTATGGTTCA ACCTCCATTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GCAATTATGC AGGGGCACTT 
ATGTACTTCT GCTCATTCTA TGTCATCATT GCCTACATCA TGCTGAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC TTTGTTTTAT TCCACTGAAG AGGACCAGCT TTTGAGTTAC 
AATGATCTTC GCCATTTTCA AATCATATGG AACATGGTAG ATGATAAAAG AGAGGGTGTG 
ATCCCCACTT TCCGAGTGAA GTTCCTGCTA CGGCTGCTGC GTGGGAGGCT GGAAGTGGAT 
CTTGATAAAG ACAAGCTCCT GTTTAAGCAT ATGTGCTATG AGATGGAGAG GCTGCACAAT 
GGTGGTGATG TCACCTTCCA TGATGTCTTA AGCATGCTCT CCTATCGCTC TGTAGACATC 
AGAAAAAGCC TGCAGCTAGA GGAGCTGCTG GCAAGGGAGC AGCTAGAATA CACCATAGAG 
GAGGAGGTGG CTAAGCAGAC TATTCGCATG TGGCTGAAGA AGTGCTTGAA ACGCATCCGG 
GCTAAACAAC AGCAGTCGTG CAGCATCATC CACAGCCTGA GAGAGAGCCA GGAGCAAGAG 
CGGAGCCGGC TATTCCTGAA TCCTCCCAGC ATTGAGACCA CCCAGCCAAG TGAGGACAGC 
AACGCCAACA GCCAGGACCA CAGTATGCAA CCTGAGACAA GCAGTCAGCA GCAGCTCTTA 
AGCCCTACTC TGTCAGACAG AGGAGGAAGC CGACAGGATG CAGCAGATAC TGGAAAACCC 
CAAAGGAAGA TTGGGCAATG GCGTCTGCCC TCAGCCCCCA AACCAATAAG CCATTCTGTA 
TCTTCGGTTA ACCTACGGTT TGGAGGAAGG ACAACGATGA AGACTGTGGT GTGCAAGATG 
AACCCCATGC CAGACACAGC TTCCTGTGGC TCTGAAGTTA AAAAGTGGTG GACCAGACAG 
CTGACCGTGG AGAGTGACGA GAGTGGAGAT GACCTCCTGG ATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_017316065.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171554.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu78792
Clone ID Related Accession (Same CDS sequence)	XM_017316065.1
Accession Version	XM_017316065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGCTAAAGCA AAGTGAAGCG AACGCGGACA CCAAAGAAAA ACTCCCTTTG 
CGCTTGAGAA TCTTCGAAAA ATTTCCAAAC AGACCGCAAA TGGTGAAAAT CTCAAAACTT 
CCTTCAGATT TTACAGTTCC TAAAATCAGG GAAAGCTTCA TGAAGCAGTT CATTGACCGC 
CAGCAACAGG ACACCTGCTG TCTCTTCAGA ATCCTCCCCT CTACCTCTTC CTCATCATGT 
GACAACCCCA AGAAGCCCAC AGCTGAAGAC AACAAATACA TTGATCAAAA ACTCCGCAAG 
TCTGTTTTCA GCATAAGGGC GAGGAACCTA CTGGAAAAGG AGACTGCAGT CACAAAAATC 
TTAAGAGCTT GCACTCGACA GCGCATGCTG AGCGGATCAT TCGAGGGGCA GCCAGCAAAG 
GAGAGGTCGA TCCTCAGCGT GCAGCATCAC ATCCGCCAGG AGCGCAGGTC ACTAAGACAT 
GGATCCAACA GCCAGAGGAT CAGCAGGGGG AAATCTCTTG AAACTTTAAC TCAAGACCAT 
TCAAATACAG TGCGCTACAG AAATGCACAA AGAGAAGACA GTGAAATAAA AATGATTCAG 
GAGAAGAAGG AACAAGCCGA GATGAAAAGG AAGGTGCAAG AAGAGGAACT GCGGGAGAAC 
CACCCATACT TTGACAAGCC TCTCTTCATC GTGGGGCGGG AGCACAGGTT CAGAAACTTC 
TGCCGCGTGG TGGTTCGAGC ACGCTTCAAT GCATCCAAAA CAGACCCTGT CACGGGAGCT 
GTGAAAAATA CAAAGTACCA CCAACTTTAT GATTTGCTGG GACTTGTCAC CTACCTGGAC 
TGGGTCATGA TCACTGTAAC CATCTGCTCT TGCATTTCTA TGATGTTCGA ATCCCCCTTC 
CGAAGAGTCA TGCATGCACC CACTTTACAG ATCGCAGAAT ATGTGTTTGT AATATTCATG 
AGCATTGAGC TTAATCTGAA GATTATGGCA GATGGCTTGT TTTTCACTCC AACTGCTGTC 
ATCAGGGACT TTGGTGGCGT CATGGACATA TTTATCTATC TTGTGAGTTT GATATTTCTT 
TGTTGGATGC CTCAAAATGT ACCTGCCGAG TCAGGAGCCC AGCTACTGAT GGTTCTTCGG 
TGCCTAAGAC CTCTTCGGAT ATTCAAACTT GTGCCCCAAA TGAGGAAAGT TGTTCGAGAA 
CTTTTCAGTG GTTTCAAGGA AATATTTTTG GTCTCCATTC TGTTGTTGAC GTTAATGCTT 
GTTTTTGCAA GCTTTGGGGT TCAGCTCTTT GCTGGAAAGT TAGCCAAGTG CAACGACCCC 
AACATTATCA GAAGGGAAGA CTGTAATGGC ATCTTCAGAA TTAATGTAAG TGTGTCCAAG 
AACTTAAATT TAAAACTAAG ACCTGGAGAG AAAAAACCTG GATTTTGGGT GCCCCGTGTT 
TGGGCAAATC CTCGGAACTT TAACTTCGAC AATGTGGGAA ATGCCATGCT GGCATTATTT 
GAAGTTCTGT CCTTGAAAGG CTGGGTAGAA GTGAGGGATG TCATTATTCA TCGTGTGGGG 
CCGATCCACG GAATCTATAT TCATGTTTTC GTATTCCTGG GTTGCATGAT CGGACTGACG 
CTTTTTGTCG GTGTAGTTAT TGCTAACTTC AATGAAAACA AGGGGACAGC CCTGCTGACG 
GTAGATCAGA GACGATGGGA AGATCTCAAG AGCAGACTGA AGATCGCACA GCCTCTTCAT 
CTCCCTCCTC GGCCGGATAA TGATGGTTTT AGAGCTAAAA TGTATGACAT AACCCAGCAT 
CCATTTTTTA AGAGGACAAT TGCATTGCTG GTTCTGGCCC AGTCTGTGTT GCTATCTGTC 
AAGTGGGATG TTGACGATCC TGTGACGGTT CCTTTGGCAA CAATGTCAGT TGTCTTCACC 
TTCATCTTTG TCTTAGAGGT TACAATGAAG ATTATAGCAA TGTCACCAGC TGGATTCTGG 
CAAAGCAGAA GAAACCGATA TGATCTCTTG GTGACATCTC TTGGTGTTGT GTGGGTGGTG 
CTCCATTTTG CTCTGCTGAA TGCATACACC TACATGATGG GAGCCTGCGT GATTGTCTTT 
AGATTTTTCT CCATCTGTGG GAAGCATGTG ACATTAAAGA TGCTCCTTCT GACTGTGGTT 
GTCAGCATGT ACAAGAGCTT TTTTATCATC GTAGGAATGT TTCTCTTGCT GCTGTGCTAT 
GCCTTTGCTG GAGTTGTTCT GTTTGGTACC GTAAAGTATG GTGAGAACAT TAACAGGCAT 
GCCAATTTTT CCTCAGCTGG CAAAGCCATT ACTGTATTGT TTCGAATTGT CACAGGTGAA 
GACTGGAACA AGATTATGCA TGATTGTATG GTTCAACCTC CATTTTGTAC TCCAGATGAA 
TTTACATACT GGGCAACAGA CTGTGGCAAT TATGCAGGGG CACTTATGTA CTTCTGCTCA 
TTCTATGTCA TCATTGCCTA CATCATGCTG AATCTGCTTG TAGCCATAAT TGTGGAGAAT 
TTCTCTTTGT TTTATTCCAC TGAAGAGGAC CAGCTTTTGA GTTACAATGA TCTTCGCCAT 
TTTCAAATCA TATGGAACAT GGTAGATGAT AAAAGAGAGG GTGTGATCCC CACTTTCCGA 
GTGAAGTTCC TGCTACGGCT GCTGCGTGGG AGGCTGGAAG TGGATCTTGA TAAAGACAAG 
CTCCTGTTTA AGCATATGTG CTATGAGATG GAGAGGCTGC ACAATGGTGG TGATGTCACC 
TTCCATGATG TCTTAAGCAT GCTCTCCTAT CGCTCTGTAG ACATCAGAAA AAGCCTGCAG 
CTAGAGGAGC TGCTGGCAAG GGAGCAGCTA GAATACACCA TAGAGGAGGA GGTGGCTAAG 
CAGACTATTC GCATGTGGCT GAAGAAGTGC TTGAAACGCA TCCGGGCTAA ACAACAGCAG 
TCGTGCAGCA TCATCCACAG CCTGAGAGAG AGCCAGGAGC AAGAGCGGAG CCGGCTATTC 
CTGAATCCTC CCAGCATTGA GACCACCCAG CCAAGTGAGG ACAGCAACGC CAACAGCCAG 
GACCACAGTA TGCAACCTGA GACAAGCAGT CAGCAGCAGC TCTTAAGCCC TACTCTGTCA 
GACAGAGGAG GAAGCCGACA GGATGCAGCA GATACTGGAA AACCCCAAAG GAAGATTGGG 
CAATGGCGTC TGCCCTCAGC CCCCAAACCA ATAAGCCATT CTGTATCTTC GGTTAACCTA 
CGGTTTGGAG GAAGGACAAC GATGAAGACT GTGGTGTGCA AGATGAACCC CATGCCAGAC 
ACAGCTTCCT GTGGCTCTGA AGTTAAAAAG TGGTGGACCA GACAGCTGAC CGTGGAGAGT 
GACGAGAGTG GAGATGACCT CCTGGATATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171554.1
CDS	87..5279
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_017316065.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316065.1

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGCTAAAGCA AAGTGAAGCG AACGCGGACA CCAAAGAAAA ACTCCCTTTG 
CGCTTGAGAA TCTTCGAAAA ATTTCCAAAC AGACCGCAAA TGGTGAAAAT CTCAAAACTT 
CCTTCAGATT TTACAGTTCC TAAAATCAGG GAAAGCTTCA TGAAGCAGTT CATTGACCGC 
CAGCAACAGG ACACCTGCTG TCTCTTCAGA ATCCTCCCCT CTACCTCTTC CTCATCATGT 
GACAACCCCA AGAAGCCCAC AGCTGAAGAC AACAAATACA TTGATCAAAA ACTCCGCAAG 
TCTGTTTTCA GCATAAGGGC GAGGAACCTA CTGGAAAAGG AGACTGCAGT CACAAAAATC 
TTAAGAGCTT GCACTCGACA GCGCATGCTG AGCGGATCAT TCGAGGGGCA GCCAGCAAAG 
GAGAGGTCGA TCCTCAGCGT GCAGCATCAC ATCCGCCAGG AGCGCAGGTC ACTAAGACAT 
GGATCCAACA GCCAGAGGAT CAGCAGGGGG AAATCTCTTG AAACTTTAAC TCAAGACCAT 
TCAAATACAG TGCGCTACAG AAATGCACAA AGAGAAGACA GTGAAATAAA AATGATTCAG 
GAGAAGAAGG AACAAGCCGA GATGAAAAGG AAGGTGCAAG AAGAGGAACT GCGGGAGAAC 
CACCCATACT TTGACAAGCC TCTCTTCATC GTGGGGCGGG AGCACAGGTT CAGAAACTTC 
TGCCGCGTGG TGGTTCGAGC ACGCTTCAAT GCATCCAAAA CAGACCCTGT CACGGGAGCT 
GTGAAAAATA CAAAGTACCA CCAACTTTAT GATTTGCTGG GACTTGTCAC CTACCTGGAC 
TGGGTCATGA TCACTGTAAC CATCTGCTCT TGCATTTCTA TGATGTTCGA ATCCCCCTTC 
CGAAGAGTCA TGCATGCACC CACTTTACAG ATCGCAGAAT ATGTGTTTGT AATATTCATG 
AGCATTGAGC TTAATCTGAA GATTATGGCA GATGGCTTGT TTTTCACTCC AACTGCTGTC 
ATCAGGGACT TTGGTGGCGT CATGGACATA TTTATCTATC TTGTGAGTTT GATATTTCTT 
TGTTGGATGC CTCAAAATGT ACCTGCCGAG TCAGGAGCCC AGCTACTGAT GGTTCTTCGG 
TGCCTAAGAC CTCTTCGGAT ATTCAAACTT GTGCCCCAAA TGAGGAAAGT TGTTCGAGAA 
CTTTTCAGTG GTTTCAAGGA AATATTTTTG GTCTCCATTC TGTTGTTGAC GTTAATGCTT 
GTTTTTGCAA GCTTTGGGGT TCAGCTCTTT GCTGGAAAGT TAGCCAAGTG CAACGACCCC 
AACATTATCA GAAGGGAAGA CTGTAATGGC ATCTTCAGAA TTAATGTAAG TGTGTCCAAG 
AACTTAAATT TAAAACTAAG ACCTGGAGAG AAAAAACCTG GATTTTGGGT GCCCCGTGTT 
TGGGCAAATC CTCGGAACTT TAACTTCGAC AATGTGGGAA ATGCCATGCT GGCATTATTT 
GAAGTTCTGT CCTTGAAAGG CTGGGTAGAA GTGAGGGATG TCATTATTCA TCGTGTGGGG 
CCGATCCACG GAATCTATAT TCATGTTTTC GTATTCCTGG GTTGCATGAT CGGACTGACG 
CTTTTTGTCG GTGTAGTTAT TGCTAACTTC AATGAAAACA AGGGGACAGC CCTGCTGACG 
GTAGATCAGA GACGATGGGA AGATCTCAAG AGCAGACTGA AGATCGCACA GCCTCTTCAT 
CTCCCTCCTC GGCCGGATAA TGATGGTTTT AGAGCTAAAA TGTATGACAT AACCCAGCAT 
CCATTTTTTA AGAGGACAAT TGCATTGCTG GTTCTGGCCC AGTCTGTGTT GCTATCTGTC 
AAGTGGGATG TTGACGATCC TGTGACGGTT CCTTTGGCAA CAATGTCAGT TGTCTTCACC 
TTCATCTTTG TCTTAGAGGT TACAATGAAG ATTATAGCAA TGTCACCAGC TGGATTCTGG 
CAAAGCAGAA GAAACCGATA TGATCTCTTG GTGACATCTC TTGGTGTTGT GTGGGTGGTG 
CTCCATTTTG CTCTGCTGAA TGCATACACC TACATGATGG GAGCCTGCGT GATTGTCTTT 
AGATTTTTCT CCATCTGTGG GAAGCATGTG ACATTAAAGA TGCTCCTTCT GACTGTGGTT 
GTCAGCATGT ACAAGAGCTT TTTTATCATC GTAGGAATGT TTCTCTTGCT GCTGTGCTAT 
GCCTTTGCTG GAGTTGTTCT GTTTGGTACC GTAAAGTATG GTGAGAACAT TAACAGGCAT 
GCCAATTTTT CCTCAGCTGG CAAAGCCATT ACTGTATTGT TTCGAATTGT CACAGGTGAA 
GACTGGAACA AGATTATGCA TGATTGTATG GTTCAACCTC CATTTTGTAC TCCAGATGAA 
TTTACATACT GGGCAACAGA CTGTGGCAAT TATGCAGGGG CACTTATGTA CTTCTGCTCA 
TTCTATGTCA TCATTGCCTA CATCATGCTG AATCTGCTTG TAGCCATAAT TGTGGAGAAT 
TTCTCTTTGT TTTATTCCAC TGAAGAGGAC CAGCTTTTGA GTTACAATGA TCTTCGCCAT 
TTTCAAATCA TATGGAACAT GGTAGATGAT AAAAGAGAGG GTGTGATCCC CACTTTCCGA 
GTGAAGTTCC TGCTACGGCT GCTGCGTGGG AGGCTGGAAG TGGATCTTGA TAAAGACAAG 
CTCCTGTTTA AGCATATGTG CTATGAGATG GAGAGGCTGC ACAATGGTGG TGATGTCACC 
TTCCATGATG TCTTAAGCAT GCTCTCCTAT CGCTCTGTAG ACATCAGAAA AAGCCTGCAG 
CTAGAGGAGC TGCTGGCAAG GGAGCAGCTA GAATACACCA TAGAGGAGGA GGTGGCTAAG 
CAGACTATTC GCATGTGGCT GAAGAAGTGC TTGAAACGCA TCCGGGCTAA ACAACAGCAG 
TCGTGCAGCA TCATCCACAG CCTGAGAGAG AGCCAGGAGC AAGAGCGGAG CCGGCTATTC 
CTGAATCCTC CCAGCATTGA GACCACCCAG CCAAGTGAGG ACAGCAACGC CAACAGCCAG 
GACCACAGTA TGCAACCTGA GACAAGCAGT CAGCAGCAGC TCTTAAGCCC TACTCTGTCA 
GACAGAGGAG GAAGCCGACA GGATGCAGCA GATACTGGAA AACCCCAAAG GAAGATTGGG 
CAATGGCGTC TGCCCTCAGC CCCCAAACCA ATAAGCCATT CTGTATCTTC GGTTAACCTA 
CGGTTTGGAG GAAGGACAAC GATGAAGACT GTGGTGTGCA AGATGAACCC CATGCCAGAC 
ACAGCTTCCT GTGGCTCTGA AGTTAAAAAG TGGTGGACCA GACAGCTGAC CGTGGAGAGT 
GACGAGAGTG GAGATGACCT CCTGGATATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_030247866.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103726.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X5, mRNA.

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CloneID	OMu86440
Clone ID Related Accession (Same CDS sequence)	XM_030247866.1
Accession Version	XM_030247866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GAAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTTACTGA CTTTGGTCCT 
GATGAATCTC TGTCAGACAA TGCTGACATA CTCTGGATTA ATAAGCCATG GGTGCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTGT GCATGAACAC ACCTATGACC 
TTTGAGCACT ATCCTCCTCT TCAGTATGTG ACCTTCACCT TGGACACTTT ATTGATGTTC 
CTCTACACTG CAGAAATGAT AGCAAAGATG CACATACGGG GAATTGTCAA GGGTGATAGC 
TCCTATGTGA AGGATCGCTG GTGTGTTTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCTCTTGTGT TACAGGTGTT TGAAATAGCT GACATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGCTGCGGA TCCCACGGCC ACTCATTATG ATCCGGGCTT TCAGGATTTA TTTCCGATTC 
GAACTGCCAA GGACAAGAAT TACAAACATT TTAAAGCGGT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTCCTTCTT CTGTATGGGA TTTTAGGAGT TCAAATGTTT 
GGAACATTTA CCTACCACTG TGTAGTCAAT GACACAAAGC CAGGAAATGT AACCTGGAAT 
AGCTTAGCTA TTCCAGATAC GCACTGCTCC CCAGAGCTAG AAGAAGGCTA TCAATGCCCG 
CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG CACGAGTATA TTCACAGTCT ATGAGGCTTC ATCTCAGGAA 
GGCTGGGTAT TCCTCATGTA CAGAGCAATC GACAGCTTTC CCCGCTGGCG CTCCTACTTC 
TACTTCATCA CACTGATTTT CTTCCTTGCT TGGCTTGTCA AGAATGTGTT TATTGCTGTC 
ATCATTGAGA CATTTGCAGA AATCAGAGTA CAATTTCAAC AAATGTGGGG AACTCGGAGC 
AGCACAACTT CTACAGCCAC CACACAGATG TTCCATGAAG ATGCCGCTGG TGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGTGCCC CAGCCTGCCT ACAGAAAATG 
ATGCGGTCAT CGGTTTTCCA CATGTTTATC CTGAGCATGG TGACTGTGGA TGTAATAGTT 
GCTGCCAGCA ATTACTACAA GGGAGAGAAC TTCAGAAGGC AGTATGATGA ATTCTACCTT 
GCAGAGGTGG CTTTTACAGT CCTTTTCGAC CTGGAAGCAC TCCTGAAGAT ATGGTGTTTG 
GGGTTTACTG GCTACATCAG CTCATCTCTC CACAAGTTTG AACTATTGCT TGTTATTGGG 
ACGACGCTTC ATGTGTACCC AGATCTTTAT CATTCTCAGT TCACATACTT CCAGGTTCTG 
CGGGTAGTCC GGCTTATTAA GATTTCCCCA GCATTGGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGGA AAAAACTTGG AAGCTTGGTG GTGTTCACTG CCAGCCTCCT GATAGTTATG 
TCAGCCATCA GTTTGCAGAT GTTCTGTTTT GTCGAAGAAC TGGACAGATT CACCACTTTT 
CCAAGGGCAT TTATGTCTAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGATGTGATG 
GATCAGACTC TGAATGCTGT GGGGCATATG TGGGCACCAC TGGTTGCCAT CTATTTCATC 
CTCTATCATC TCTTTGCAAC TTTGCTAAAG CAAAGTGAAG CGAACGCGGA CACCAAAGAA 
AAACTCCCTT TGCGCTTGAG AATCTTCGAA AAATTTCCAA ACAGACCGCA AATGGTGAAA 
ATCTCAAAAC TTCCTTCAGA TTTTACAGTT CCTAAAATCA GGGAAAGCTT CATGAAGCAG 
TTCATTGACC GCCAGCAACA GGACACCTGC TGTCTCTTCA GAATCCTCCC CTCTACCTCT 
TCCTCATCAT GTGACAACCC CAAGAAGCCC ACAGCTGAAG ACAACAAATA CATTGATCAA 
AAACTCCGCA AGTCTGTTTT CAGCATAAGG GCGAGGAACC TACTGGAAAA GGAGACTGCA 
GTCACAAAAA TCTTAAGAGC TTGCACTCGA CAGCGCATGC TGAGCGGATC ATTCGAGGGG 
CAGCCAGCAA AGGAGAGGTC GATCCTCAGC GTGCAGCATC ACATCCGCCA GGAGCGCAGG 
TCACTAAGAC ATGGATCCAA CAGCCAGAGG ATCAGCAGGG GGAAATCTCT TGAAACTTTA 
ACTCAAGACC ATTCAAATAC AGTGCGCTAC AGAAATGCAC AAAGAGAAGA CAGTGAAATA 
AAAATGATTC AGGAGAAGAA GGAACAAGCC GAGATGAAAA GGAAGGTGCA AGAAGAGGAA 
CTGCGGGAGA ACCACCCATA CTTTGACAAG CCTCTCTTCA TCGTGGGGCG GGAGCACAGG 
TTCAGAAACT TCTGCCGCGT GGTGGTTCGA GCACGCTTCA ATGCATCCAA AACAGACCCT 
GTCACGGGAG CTGTGAAAAA TACAAAGTAC CACCAACTTT ATGATTTGCT GGGACTTGTC 
ACCTACCTGG ACTGGGTCAT GATCACTGTA ACCATCTGCT CTTGCATTTC TATGATGTTC 
GAATCCCCCT TCCGAAGAGT CATGCATGCA CCCACTTTAC AGATCGCAGA ATATGTGTTT 
GTAATATTCA TGAGCATTGA GCTTAATCTG AAGATTATGG CAGATGGCTT GTTTTTCACT 
CCAACTGCTG TCATCAGGGA CTTTGGTGGC GTCATGGACA TATTTATCTA TCTTGTGAGT 
TTGATATTTC TTTGTTGGAT GCCTCAAAAT GTACCTGCCG AGTCAGGAGC CCAGCTACTG 
ATGGTTCTTC GGTGCCTAAG ACCTCTTCGG ATATTCAAAC TTGTGCCCCA AATGAGGAAA 
GTTGTTCGAG AACTTTTCAG TGGTTTCAAG GAAATATTTT TGGTCTCCAT TCTGTTGTTG 
ACGTTAATGC TTGTTTTTGC AAGCTTTGGG GTTCAGCTCT TTGCTGGAAA GTTAGCCAAG 
TGCAACGACC CCAACATTAT CAGAAGGGAA GACTGTAATG GCATCTTCAG AATTAATGTA 
AGTGTGTCCA AGAACTTAAA TTTAAAACTA AGACCTGGAG AGAAAAAACC TGGATTTTGG 
GTGCCCCGTG TTTGGGCAAA TCCTCGGAAC TTTAACTTCG ACAATGTGGG AAATGCCATG 
CTGGCATTAT TTGAAGTTCT GTCCTTGAAA GGCTGGGTAG AAGTGAGGGA TGTCATTATT 
CATCGTGTGG GGCCGATCCA CGGAATCTAT ATTCATGTTT TCGTATTCCT GGGTTGCATG 
ATCGGACTGA CGCTTTTTGT CGGTGTAGTT ATTGCTAACT TCAATGAAAA CAAGGGGACA 
GCCCTGCTGA CGGTAGATCA GAGACGATGG GAAGATCTCA AGAGCAGACT GAAGATCGCA 
CAGCCTCTTC ATCTCCCTCC TCGGCCGGAT AATGATGGTT TTAGAGCTAA AATGTATGAC 
ATAACCCAGC ATCCATTTTT TAAGAGGACA ATTGCATTGC TGGTTCTGGC CCAGTCTGTG 
TTGCTATCTG TCAAGTGGGA TGTTGACGAT CCTGTGACGG TTCCTTTGGC AACAATGTCA 
GTTGTCTTCA CCTTCATCTT TGTCTTAGAG GTTACAATGA AGATTATAGC AATGTCACCA 
GCTGGATTCT GGCAAAGCAG AAGAAACCGA TATGATCTCT TGGTGACATC TCTTGGTGTT 
GTGTGGGTGG TGCTCCATTT TGCTCTGCTG AATGCATACA CCTACATGAT GGGAGCCTGC 
GTGATTGTCT TTAGATTTTT CTCCATCTGT GGGAAGCATG TGACATTAAA GATGCTCCTT 
CTGACTGTGG TTGTCAGCAT GTACAAGAGC TTTTTTATCA TCGTAGGAAT GTTTCTCTTG 
CTGCTGTGCT ATGCCTTTGC TGGAGTTGTT CTGTTTGGTA CCGTAAAGTA TGGTGAGAAC 
ATTAACAGGC ATGCCAATTT TTCCTCAGCT GGCAAAGCCA TTACTGTATT GTTTCGAATT 
GTCACAGGTG AAGACTGGAA CAAGATTATG CATGATTGTA TGGTTCAACC TCCATTTTGT 
ACTCCAGATG AATTTACATA CTGGGCAACA GACTGTGGCA ATTATGCAGG GGCACTTATG 
TACTTCTGCT CATTCTATGT CATCATTGCC TACATCATGC TGAATCTGCT TGTAGCCATA 
ATTGTGGAGA ATTTCTCTTT GTTTTATTCC ACTGAAGAGG ACCAGCTTTT GAGTTACAAT 
GATCTTCGCC ATTTTCAAAT CATATGGAAC ATGGTAGATG ATAAAAGAGA GGGTGTGATC 
CCCACTTTCC GAGTGAAGTT CCTGCTACGG CTGCTGCGTG GGAGGCTGGA AGTGGATCTT 
GATAAAGACA AGCTCCTGTT TAAGCATATG TGCTATGAGA TGGAGAGGCT GCACAATGGT 
GGTGATGTCA CCTTCCATGA TGTCTTAAGC ATGCTCTCCT ATCGCTCTGT AGACATCAGA 
AAAAGCCTGC AGCTAGAGGA GCTGCTGGCA AGGGAGCAGC TAGAATACAC CATAGAGGAG 
GAGGTGGCTA AGCAGACTAT TCGCATGTGG CTGAAGAAGT GCTTGAAACG CATCCGGGCT 
AAACAACAGC AGTCGTGCAG CATCATCCAC AGCCTGAGAG AGAGCCAGGA GCAAGAGCGG 
AGCCGGCTAT TCCTGAATCC TCCCAGCATT GAGACCACCC AGCCAAGTGA GGACAGCAAC 
GCCAACAGCC AGGACCACAG TATGCAACCT GAGACAAGCA GTCAGCAGCA GCTCTTAAGC 
CCTACTCTGT CAGACAGAGG AGGAAGCCGA CAGGATGCAG CAGATACTGG AAAACCCCAA 
AGGAAGATTG GGCAATGGCG TCTGCCCTCA GCTGCCATCG CTGAGAAGAC GGCACCTGTT 
AACAAAATGA GCGGTTACAG CCAAGATCCA GAAAGAGAGG AGGAAGAGGT TTCCAAGGGC 
TTCCTGGCCC CCAAACCAAT AAGCCATTCT GTATCTTCGG TTAACCTACG GTTTGGAGGA 
AGGACAACGA TGAAGACTGT GGTGTGCAAG ATGAACCCCA TGCCAGACAC AGCTTCCTGT 
GGCTCTGAAG TTAAAAAGTG GTGGACCAGA CAGCTGACCG TGGAGAGTGA CGAGAGTGGA 
GATGACCTCC TGGATATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103726.1
CDS	470..5710
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAAIAEKTAPVNKMSGYSQDPEREEEEVSKGFLAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_030247866.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247866.1

ORF Insert Sequence:

ATGCTCAAAA GAAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTTACTGA CTTTGGTCCT 
GATGAATCTC TGTCAGACAA TGCTGACATA CTCTGGATTA ATAAGCCATG GGTGCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTGT GCATGAACAC ACCTATGACC 
TTTGAGCACT ATCCTCCTCT TCAGTATGTG ACCTTCACCT TGGACACTTT ATTGATGTTC 
CTCTACACTG CAGAAATGAT AGCAAAGATG CACATACGGG GAATTGTCAA GGGTGATAGC 
TCCTATGTGA AGGATCGCTG GTGTGTTTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCTCTTGTGT TACAGGTGTT TGAAATAGCT GACATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGCTGCGGA TCCCACGGCC ACTCATTATG ATCCGGGCTT TCAGGATTTA TTTCCGATTC 
GAACTGCCAA GGACAAGAAT TACAAACATT TTAAAGCGGT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTCCTTCTT CTGTATGGGA TTTTAGGAGT TCAAATGTTT 
GGAACATTTA CCTACCACTG TGTAGTCAAT GACACAAAGC CAGGAAATGT AACCTGGAAT 
AGCTTAGCTA TTCCAGATAC GCACTGCTCC CCAGAGCTAG AAGAAGGCTA TCAATGCCCG 
CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG CACGAGTATA TTCACAGTCT ATGAGGCTTC ATCTCAGGAA 
GGCTGGGTAT TCCTCATGTA CAGAGCAATC GACAGCTTTC CCCGCTGGCG CTCCTACTTC 
TACTTCATCA CACTGATTTT CTTCCTTGCT TGGCTTGTCA AGAATGTGTT TATTGCTGTC 
ATCATTGAGA CATTTGCAGA AATCAGAGTA CAATTTCAAC AAATGTGGGG AACTCGGAGC 
AGCACAACTT CTACAGCCAC CACACAGATG TTCCATGAAG ATGCCGCTGG TGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGTGCCC CAGCCTGCCT ACAGAAAATG 
ATGCGGTCAT CGGTTTTCCA CATGTTTATC CTGAGCATGG TGACTGTGGA TGTAATAGTT 
GCTGCCAGCA ATTACTACAA GGGAGAGAAC TTCAGAAGGC AGTATGATGA ATTCTACCTT 
GCAGAGGTGG CTTTTACAGT CCTTTTCGAC CTGGAAGCAC TCCTGAAGAT ATGGTGTTTG 
GGGTTTACTG GCTACATCAG CTCATCTCTC CACAAGTTTG AACTATTGCT TGTTATTGGG 
ACGACGCTTC ATGTGTACCC AGATCTTTAT CATTCTCAGT TCACATACTT CCAGGTTCTG 
CGGGTAGTCC GGCTTATTAA GATTTCCCCA GCATTGGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGGA AAAAACTTGG AAGCTTGGTG GTGTTCACTG CCAGCCTCCT GATAGTTATG 
TCAGCCATCA GTTTGCAGAT GTTCTGTTTT GTCGAAGAAC TGGACAGATT CACCACTTTT 
CCAAGGGCAT TTATGTCTAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGATGTGATG 
GATCAGACTC TGAATGCTGT GGGGCATATG TGGGCACCAC TGGTTGCCAT CTATTTCATC 
CTCTATCATC TCTTTGCAAC TTTGCTAAAG CAAAGTGAAG CGAACGCGGA CACCAAAGAA 
AAACTCCCTT TGCGCTTGAG AATCTTCGAA AAATTTCCAA ACAGACCGCA AATGGTGAAA 
ATCTCAAAAC TTCCTTCAGA TTTTACAGTT CCTAAAATCA GGGAAAGCTT CATGAAGCAG 
TTCATTGACC GCCAGCAACA GGACACCTGC TGTCTCTTCA GAATCCTCCC CTCTACCTCT 
TCCTCATCAT GTGACAACCC CAAGAAGCCC ACAGCTGAAG ACAACAAATA CATTGATCAA 
AAACTCCGCA AGTCTGTTTT CAGCATAAGG GCGAGGAACC TACTGGAAAA GGAGACTGCA 
GTCACAAAAA TCTTAAGAGC TTGCACTCGA CAGCGCATGC TGAGCGGATC ATTCGAGGGG 
CAGCCAGCAA AGGAGAGGTC GATCCTCAGC GTGCAGCATC ACATCCGCCA GGAGCGCAGG 
TCACTAAGAC ATGGATCCAA CAGCCAGAGG ATCAGCAGGG GGAAATCTCT TGAAACTTTA 
ACTCAAGACC ATTCAAATAC AGTGCGCTAC AGAAATGCAC AAAGAGAAGA CAGTGAAATA 
AAAATGATTC AGGAGAAGAA GGAACAAGCC GAGATGAAAA GGAAGGTGCA AGAAGAGGAA 
CTGCGGGAGA ACCACCCATA CTTTGACAAG CCTCTCTTCA TCGTGGGGCG GGAGCACAGG 
TTCAGAAACT TCTGCCGCGT GGTGGTTCGA GCACGCTTCA ATGCATCCAA AACAGACCCT 
GTCACGGGAG CTGTGAAAAA TACAAAGTAC CACCAACTTT ATGATTTGCT GGGACTTGTC 
ACCTACCTGG ACTGGGTCAT GATCACTGTA ACCATCTGCT CTTGCATTTC TATGATGTTC 
GAATCCCCCT TCCGAAGAGT CATGCATGCA CCCACTTTAC AGATCGCAGA ATATGTGTTT 
GTAATATTCA TGAGCATTGA GCTTAATCTG AAGATTATGG CAGATGGCTT GTTTTTCACT 
CCAACTGCTG TCATCAGGGA CTTTGGTGGC GTCATGGACA TATTTATCTA TCTTGTGAGT 
TTGATATTTC TTTGTTGGAT GCCTCAAAAT GTACCTGCCG AGTCAGGAGC CCAGCTACTG 
ATGGTTCTTC GGTGCCTAAG ACCTCTTCGG ATATTCAAAC TTGTGCCCCA AATGAGGAAA 
GTTGTTCGAG AACTTTTCAG TGGTTTCAAG GAAATATTTT TGGTCTCCAT TCTGTTGTTG 
ACGTTAATGC TTGTTTTTGC AAGCTTTGGG GTTCAGCTCT TTGCTGGAAA GTTAGCCAAG 
TGCAACGACC CCAACATTAT CAGAAGGGAA GACTGTAATG GCATCTTCAG AATTAATGTA 
AGTGTGTCCA AGAACTTAAA TTTAAAACTA AGACCTGGAG AGAAAAAACC TGGATTTTGG 
GTGCCCCGTG TTTGGGCAAA TCCTCGGAAC TTTAACTTCG ACAATGTGGG AAATGCCATG 
CTGGCATTAT TTGAAGTTCT GTCCTTGAAA GGCTGGGTAG AAGTGAGGGA TGTCATTATT 
CATCGTGTGG GGCCGATCCA CGGAATCTAT ATTCATGTTT TCGTATTCCT GGGTTGCATG 
ATCGGACTGA CGCTTTTTGT CGGTGTAGTT ATTGCTAACT TCAATGAAAA CAAGGGGACA 
GCCCTGCTGA CGGTAGATCA GAGACGATGG GAAGATCTCA AGAGCAGACT GAAGATCGCA 
CAGCCTCTTC ATCTCCCTCC TCGGCCGGAT AATGATGGTT TTAGAGCTAA AATGTATGAC 
ATAACCCAGC ATCCATTTTT TAAGAGGACA ATTGCATTGC TGGTTCTGGC CCAGTCTGTG 
TTGCTATCTG TCAAGTGGGA TGTTGACGAT CCTGTGACGG TTCCTTTGGC AACAATGTCA 
GTTGTCTTCA CCTTCATCTT TGTCTTAGAG GTTACAATGA AGATTATAGC AATGTCACCA 
GCTGGATTCT GGCAAAGCAG AAGAAACCGA TATGATCTCT TGGTGACATC TCTTGGTGTT 
GTGTGGGTGG TGCTCCATTT TGCTCTGCTG AATGCATACA CCTACATGAT GGGAGCCTGC 
GTGATTGTCT TTAGATTTTT CTCCATCTGT GGGAAGCATG TGACATTAAA GATGCTCCTT 
CTGACTGTGG TTGTCAGCAT GTACAAGAGC TTTTTTATCA TCGTAGGAAT GTTTCTCTTG 
CTGCTGTGCT ATGCCTTTGC TGGAGTTGTT CTGTTTGGTA CCGTAAAGTA TGGTGAGAAC 
ATTAACAGGC ATGCCAATTT TTCCTCAGCT GGCAAAGCCA TTACTGTATT GTTTCGAATT 
GTCACAGGTG AAGACTGGAA CAAGATTATG CATGATTGTA TGGTTCAACC TCCATTTTGT 
ACTCCAGATG AATTTACATA CTGGGCAACA GACTGTGGCA ATTATGCAGG GGCACTTATG 
TACTTCTGCT CATTCTATGT CATCATTGCC TACATCATGC TGAATCTGCT TGTAGCCATA 
ATTGTGGAGA ATTTCTCTTT GTTTTATTCC ACTGAAGAGG ACCAGCTTTT GAGTTACAAT 
GATCTTCGCC ATTTTCAAAT CATATGGAAC ATGGTAGATG ATAAAAGAGA GGGTGTGATC 
CCCACTTTCC GAGTGAAGTT CCTGCTACGG CTGCTGCGTG GGAGGCTGGA AGTGGATCTT 
GATAAAGACA AGCTCCTGTT TAAGCATATG TGCTATGAGA TGGAGAGGCT GCACAATGGT 
GGTGATGTCA CCTTCCATGA TGTCTTAAGC ATGCTCTCCT ATCGCTCTGT AGACATCAGA 
AAAAGCCTGC AGCTAGAGGA GCTGCTGGCA AGGGAGCAGC TAGAATACAC CATAGAGGAG 
GAGGTGGCTA AGCAGACTAT TCGCATGTGG CTGAAGAAGT GCTTGAAACG CATCCGGGCT 
AAACAACAGC AGTCGTGCAG CATCATCCAC AGCCTGAGAG AGAGCCAGGA GCAAGAGCGG 
AGCCGGCTAT TCCTGAATCC TCCCAGCATT GAGACCACCC AGCCAAGTGA GGACAGCAAC 
GCCAACAGCC AGGACCACAG TATGCAACCT GAGACAAGCA GTCAGCAGCA GCTCTTAAGC 
CCTACTCTGT CAGACAGAGG AGGAAGCCGA CAGGATGCAG CAGATACTGG AAAACCCCAA 
AGGAAGATTG GGCAATGGCG TCTGCCCTCA GCTGCCATCG CTGAGAAGAC GGCACCTGTT 
AACAAAATGA GCGGTTACAG CCAAGATCCA GAAAGAGAGG AGGAAGAGGT TTCCAAGGGC 
TTCCTGGCCC CCAAACCAAT AAGCCATTCT GTATCTTCGG TTAACCTACG GTTTGGAGGA 
AGGACAACGA TGAAGACTGT GGTGTGCAAG ATGAACCCCA TGCCAGACAC AGCTTCCTGT 
GGCTCTGAAG TTAAAAAGTG GTGGACCAGA CAGCTGACCG TGGAGAGTGA CGAGAGTGGA 
GATGACCTCC TGGATATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( NM_177393.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796367.3 Mus musculus sodium leak channel, non-selective (Nalcn), mRNA.

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CloneID	OMu20803
Clone ID Related Accession (Same CDS sequence)	NM_177393.4
Accession Version	NM_177393.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143219.1, AK043071.1, CD802755.1, AK044431.1 and AC124818.7. On Jan 19, 2007 this sequence version replaced NM_177393.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCAAAA GAAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTTACTGA CTTTGGTCCT 
GATGAATCTC TGTCAGACAA TGCTGACATA CTCTGGATTA ATAAGCCATG GGTGCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTGT GCATGAACAC ACCTATGACC 
TTTGAGCACT ATCCTCCTCT TCAGTATGTG ACCTTCACCT TGGACACTTT ATTGATGTTC 
CTCTACACTG CAGAAATGAT AGCAAAGATG CACATACGGG GAATTGTCAA GGGTGATAGC 
TCCTATGTGA AGGATCGCTG GTGTGTTTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCTCTTGTGT TACAGGTGTT TGAAATAGCT GACATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGCTGCGGA TCCCACGGCC ACTCATTATG ATCCGGGCTT TCAGGATTTA TTTCCGATTC 
GAACTGCCAA GGACAAGAAT TACAAACATT TTAAAGCGGT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTCCTTCTT CTGTATGGGA TTTTAGGAGT TCAAATGTTT 
GGAACATTTA CCTACCACTG TGTAGTCAAT GACACAAAGC CAGGAAATGT AACCTGGAAT 
AGCTTAGCTA TTCCAGATAC GCACTGCTCC CCAGAGCTAG AAGAAGGCTA TCAATGCCCG 
CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG CACGAGTATA TTCACAGTCT ATGAGGCTTC ATCTCAGGAA 
GGCTGGGTAT TCCTCATGTA CAGAGCAATC GACAGCTTTC CCCGCTGGCG CTCCTACTTC 
TACTTCATCA CACTGATTTT CTTCCTTGCT TGGCTTGTCA AGAATGTGTT TATTGCTGTC 
ATCATTGAGA CATTTGCAGA AATCAGAGTA CAATTTCAAC AAATGTGGGG AACTCGGAGC 
AGCACAACTT CTACAGCCAC CACACAGATG TTCCATGAAG ATGCCGCTGG TGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGTGCCC CAGCCTGCCT ACAGAAAATG 
ATGCGGTCAT CGGTTTTCCA CATGTTTATC CTGAGCATGG TGACTGTGGA TGTAATAGTT 
GCTGCCAGCA ATTACTACAA GGGAGAGAAC TTCAGAAGGC AGTATGATGA ATTCTACCTT 
GCAGAGGTGG CTTTTACAGT CCTTTTCGAC CTGGAAGCAC TCCTGAAGAT ATGGTGTTTG 
GGGTTTACTG GCTACATCAG CTCATCTCTC CACAAGTTTG AACTATTGCT TGTTATTGGG 
ACGACGCTTC ATGTGTACCC AGATCTTTAT CATTCTCAGT TCACATACTT CCAGGTTCTG 
CGGGTAGTCC GGCTTATTAA GATTTCCCCA GCATTGGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGGA AAAAACTTGG AAGCTTGGTG GTGTTCACTG CCAGCCTCCT GATAGTTATG 
TCAGCCATCA GTTTGCAGAT GTTCTGTTTT GTCGAAGAAC TGGACAGATT CACCACTTTT 
CCAAGGGCAT TTATGTCTAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGATGTGATG 
GATCAGACTC TGAATGCTGT GGGGCATATG TGGGCACCAC TGGTTGCCAT CTATTTCATC 
CTCTATCATC TCTTTGCAAC TTTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA TCTAAAGAAG CTCAAACAAC TAAAGCAAAG TGAAGCGAAC 
GCGGACACCA AAGAAAAACT CCCTTTGCGC TTGAGAATCT TCGAAAAATT TCCAAACAGA 
CCGCAAATGG TGAAAATCTC AAAACTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGCTTCATGA AGCAGTTCAT TGACCGCCAG CAACAGGACA CCTGCTGTCT CTTCAGAATC 
CTCCCCTCTA CCTCTTCCTC ATCATGTGAC AACCCCAAGA AGCCCACAGC TGAAGACAAC 
AAATACATTG ATCAAAAACT CCGCAAGTCT GTTTTCAGCA TAAGGGCGAG GAACCTACTG 
GAAAAGGAGA CTGCAGTCAC AAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC AGCAAAGGAG AGGTCGATCC TCAGCGTGCA GCATCACATC 
CGCCAGGAGC GCAGGTCACT AAGACATGGA TCCAACAGCC AGAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTTTAACTCA AGACCATTCA AATACAGTGC GCTACAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAAAT GATTCAGGAG AAGAAGGAAC AAGCCGAGAT GAAAAGGAAG 
GTGCAAGAAG AGGAACTGCG GGAGAACCAC CCATACTTTG ACAAGCCTCT CTTCATCGTG 
GGGCGGGAGC ACAGGTTCAG AAACTTCTGC CGCGTGGTGG TTCGAGCACG CTTCAATGCA 
TCCAAAACAG ACCCTGTCAC GGGAGCTGTG AAAAATACAA AGTACCACCA ACTTTATGAT 
TTGCTGGGAC TTGTCACCTA CCTGGACTGG GTCATGATCA CTGTAACCAT CTGCTCTTGC 
ATTTCTATGA TGTTCGAATC CCCCTTCCGA AGAGTCATGC ATGCACCCAC TTTACAGATC 
GCAGAATATG TGTTTGTAAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTGTTTT TCACTCCAAC TGCTGTCATC AGGGACTTTG GTGGCGTCAT GGACATATTT 
ATCTATCTTG TGAGTTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCCGAGTCA 
GGAGCCCAGC TACTGATGGT TCTTCGGTGC CTAAGACCTC TTCGGATATT CAAACTTGTG 
CCCCAAATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGTT TCAAGGAAAT ATTTTTGGTC 
TCCATTCTGT TGTTGACGTT AATGCTTGTT TTTGCAAGCT TTGGGGTTCA GCTCTTTGCT 
GGAAAGTTAG CCAAGTGCAA CGACCCCAAC ATTATCAGAA GGGAAGACTG TAATGGCATC 
TTCAGAATTA ATGTAAGTGT GTCCAAGAAC TTAAATTTAA AACTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTCGACAAT 
GTGGGAAATG CCATGCTGGC ATTATTTGAA GTTCTGTCCT TGAAAGGCTG GGTAGAAGTG 
AGGGATGTCA TTATTCATCG TGTGGGGCCG ATCCACGGAA TCTATATTCA TGTTTTCGTA 
TTCCTGGGTT GCATGATCGG ACTGACGCTT TTTGTCGGTG TAGTTATTGC TAACTTCAAT 
GAAAACAAGG GGACAGCCCT GCTGACGGTA GATCAGAGAC GATGGGAAGA TCTCAAGAGC 
AGACTGAAGA TCGCACAGCC TCTTCATCTC CCTCCTCGGC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATTGC ATTGCTGGTT 
CTGGCCCAGT CTGTGTTGCT ATCTGTCAAG TGGGATGTTG ACGATCCTGT GACGGTTCCT 
TTGGCAACAA TGTCAGTTGT CTTCACCTTC ATCTTTGTCT TAGAGGTTAC AATGAAGATT 
ATAGCAATGT CACCAGCTGG ATTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCTTGGTG 
ACATCTCTTG GTGTTGTGTG GGTGGTGCTC CATTTTGCTC TGCTGAATGC ATACACCTAC 
ATGATGGGAG CCTGCGTGAT TGTCTTTAGA TTTTTCTCCA TCTGTGGGAA GCATGTGACA 
TTAAAGATGC TCCTTCTGAC TGTGGTTGTC AGCATGTACA AGAGCTTTTT TATCATCGTA 
GGAATGTTTC TCTTGCTGCT GTGCTATGCC TTTGCTGGAG TTGTTCTGTT TGGTACCGTA 
AAGTATGGTG AGAACATTAA CAGGCATGCC AATTTTTCCT CAGCTGGCAA AGCCATTACT 
GTATTGTTTC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA TTGTATGGTT 
CAACCTCCAT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGCAATTAT 
GCAGGGGCAC TTATGTACTT CTGCTCATTC TATGTCATCA TTGCCTACAT CATGCTGAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCTTTGTTTT ATTCCACTGA AGAGGACCAG 
CTTTTGAGTT ACAATGATCT TCGCCATTTT CAAATCATAT GGAACATGGT AGATGATAAA 
AGAGAGGGTG TGATCCCCAC TTTCCGAGTG AAGTTCCTGC TACGGCTGCT GCGTGGGAGG 
CTGGAAGTGG ATCTTGATAA AGACAAGCTC CTGTTTAAGC ATATGTGCTA TGAGATGGAG 
AGGCTGCACA ATGGTGGTGA TGTCACCTTC CATGATGTCT TAAGCATGCT CTCCTATCGC 
TCTGTAGACA TCAGAAAAAG CCTGCAGCTA GAGGAGCTGC TGGCAAGGGA GCAGCTAGAA 
TACACCATAG AGGAGGAGGT GGCTAAGCAG ACTATTCGCA TGTGGCTGAA GAAGTGCTTG 
AAACGCATCC GGGCTAAACA ACAGCAGTCG TGCAGCATCA TCCACAGCCT GAGAGAGAGC 
CAGGAGCAAG AGCGGAGCCG GCTATTCCTG AATCCTCCCA GCATTGAGAC CACCCAGCCA 
AGTGAGGACA GCAACGCCAA CAGCCAGGAC CACAGTATGC AACCTGAGAC AAGCAGTCAG 
CAGCAGCTCT TAAGCCCTAC TCTGTCAGAC AGAGGAGGAA GCCGACAGGA TGCAGCAGAT 
ACTGGAAAAC CCCAAAGGAA GATTGGGCAA TGGCGTCTGC CCTCAGCCCC CAAACCAATA 
AGCCATTCTG TATCTTCGGT TAACCTACGG TTTGGAGGAA GGACAACGAT GAAGACTGTG 
GTGTGCAAGA TGAACCCCAT GCCAGACACA GCTTCCTGTG GCTCTGAAGT TAAAAAGTGG 
TGGACCAGAC AGCTGACCGT GGAGAGTGAC GAGAGTGGAG ATGACCTCCT GGATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796367.3
CDS	369..5588
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	NM_177393.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177393.4

ORF Insert Sequence:

ATGCTCAAAA GAAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTTACTGA CTTTGGTCCT 
GATGAATCTC TGTCAGACAA TGCTGACATA CTCTGGATTA ATAAGCCATG GGTGCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTGT GCATGAACAC ACCTATGACC 
TTTGAGCACT ATCCTCCTCT TCAGTATGTG ACCTTCACCT TGGACACTTT ATTGATGTTC 
CTCTACACTG CAGAAATGAT AGCAAAGATG CACATACGGG GAATTGTCAA GGGTGATAGC 
TCCTATGTGA AGGATCGCTG GTGTGTTTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCTCTTGTGT TACAGGTGTT TGAAATAGCT GACATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGCTGCGGA TCCCACGGCC ACTCATTATG ATCCGGGCTT TCAGGATTTA TTTCCGATTC 
GAACTGCCAA GGACAAGAAT TACAAACATT TTAAAGCGGT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTCCTTCTT CTGTATGGGA TTTTAGGAGT TCAAATGTTT 
GGAACATTTA CCTACCACTG TGTAGTCAAT GACACAAAGC CAGGAAATGT AACCTGGAAT 
AGCTTAGCTA TTCCAGATAC GCACTGCTCC CCAGAGCTAG AAGAAGGCTA TCAATGCCCG 
CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG CACGAGTATA TTCACAGTCT ATGAGGCTTC ATCTCAGGAA 
GGCTGGGTAT TCCTCATGTA CAGAGCAATC GACAGCTTTC CCCGCTGGCG CTCCTACTTC 
TACTTCATCA CACTGATTTT CTTCCTTGCT TGGCTTGTCA AGAATGTGTT TATTGCTGTC 
ATCATTGAGA CATTTGCAGA AATCAGAGTA CAATTTCAAC AAATGTGGGG AACTCGGAGC 
AGCACAACTT CTACAGCCAC CACACAGATG TTCCATGAAG ATGCCGCTGG TGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGTGCCC CAGCCTGCCT ACAGAAAATG 
ATGCGGTCAT CGGTTTTCCA CATGTTTATC CTGAGCATGG TGACTGTGGA TGTAATAGTT 
GCTGCCAGCA ATTACTACAA GGGAGAGAAC TTCAGAAGGC AGTATGATGA ATTCTACCTT 
GCAGAGGTGG CTTTTACAGT CCTTTTCGAC CTGGAAGCAC TCCTGAAGAT ATGGTGTTTG 
GGGTTTACTG GCTACATCAG CTCATCTCTC CACAAGTTTG AACTATTGCT TGTTATTGGG 
ACGACGCTTC ATGTGTACCC AGATCTTTAT CATTCTCAGT TCACATACTT CCAGGTTCTG 
CGGGTAGTCC GGCTTATTAA GATTTCCCCA GCATTGGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGGA AAAAACTTGG AAGCTTGGTG GTGTTCACTG CCAGCCTCCT GATAGTTATG 
TCAGCCATCA GTTTGCAGAT GTTCTGTTTT GTCGAAGAAC TGGACAGATT CACCACTTTT 
CCAAGGGCAT TTATGTCTAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGATGTGATG 
GATCAGACTC TGAATGCTGT GGGGCATATG TGGGCACCAC TGGTTGCCAT CTATTTCATC 
CTCTATCATC TCTTTGCAAC TTTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA TCTAAAGAAG CTCAAACAAC TAAAGCAAAG TGAAGCGAAC 
GCGGACACCA AAGAAAAACT CCCTTTGCGC TTGAGAATCT TCGAAAAATT TCCAAACAGA 
CCGCAAATGG TGAAAATCTC AAAACTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGCTTCATGA AGCAGTTCAT TGACCGCCAG CAACAGGACA CCTGCTGTCT CTTCAGAATC 
CTCCCCTCTA CCTCTTCCTC ATCATGTGAC AACCCCAAGA AGCCCACAGC TGAAGACAAC 
AAATACATTG ATCAAAAACT CCGCAAGTCT GTTTTCAGCA TAAGGGCGAG GAACCTACTG 
GAAAAGGAGA CTGCAGTCAC AAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC AGCAAAGGAG AGGTCGATCC TCAGCGTGCA GCATCACATC 
CGCCAGGAGC GCAGGTCACT AAGACATGGA TCCAACAGCC AGAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTTTAACTCA AGACCATTCA AATACAGTGC GCTACAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAAAT GATTCAGGAG AAGAAGGAAC AAGCCGAGAT GAAAAGGAAG 
GTGCAAGAAG AGGAACTGCG GGAGAACCAC CCATACTTTG ACAAGCCTCT CTTCATCGTG 
GGGCGGGAGC ACAGGTTCAG AAACTTCTGC CGCGTGGTGG TTCGAGCACG CTTCAATGCA 
TCCAAAACAG ACCCTGTCAC GGGAGCTGTG AAAAATACAA AGTACCACCA ACTTTATGAT 
TTGCTGGGAC TTGTCACCTA CCTGGACTGG GTCATGATCA CTGTAACCAT CTGCTCTTGC 
ATTTCTATGA TGTTCGAATC CCCCTTCCGA AGAGTCATGC ATGCACCCAC TTTACAGATC 
GCAGAATATG TGTTTGTAAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTGTTTT TCACTCCAAC TGCTGTCATC AGGGACTTTG GTGGCGTCAT GGACATATTT 
ATCTATCTTG TGAGTTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCCGAGTCA 
GGAGCCCAGC TACTGATGGT TCTTCGGTGC CTAAGACCTC TTCGGATATT CAAACTTGTG 
CCCCAAATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGTT TCAAGGAAAT ATTTTTGGTC 
TCCATTCTGT TGTTGACGTT AATGCTTGTT TTTGCAAGCT TTGGGGTTCA GCTCTTTGCT 
GGAAAGTTAG CCAAGTGCAA CGACCCCAAC ATTATCAGAA GGGAAGACTG TAATGGCATC 
TTCAGAATTA ATGTAAGTGT GTCCAAGAAC TTAAATTTAA AACTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTCGACAAT 
GTGGGAAATG CCATGCTGGC ATTATTTGAA GTTCTGTCCT TGAAAGGCTG GGTAGAAGTG 
AGGGATGTCA TTATTCATCG TGTGGGGCCG ATCCACGGAA TCTATATTCA TGTTTTCGTA 
TTCCTGGGTT GCATGATCGG ACTGACGCTT TTTGTCGGTG TAGTTATTGC TAACTTCAAT 
GAAAACAAGG GGACAGCCCT GCTGACGGTA GATCAGAGAC GATGGGAAGA TCTCAAGAGC 
AGACTGAAGA TCGCACAGCC TCTTCATCTC CCTCCTCGGC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATTGC ATTGCTGGTT 
CTGGCCCAGT CTGTGTTGCT ATCTGTCAAG TGGGATGTTG ACGATCCTGT GACGGTTCCT 
TTGGCAACAA TGTCAGTTGT CTTCACCTTC ATCTTTGTCT TAGAGGTTAC AATGAAGATT 
ATAGCAATGT CACCAGCTGG ATTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCTTGGTG 
ACATCTCTTG GTGTTGTGTG GGTGGTGCTC CATTTTGCTC TGCTGAATGC ATACACCTAC 
ATGATGGGAG CCTGCGTGAT TGTCTTTAGA TTTTTCTCCA TCTGTGGGAA GCATGTGACA 
TTAAAGATGC TCCTTCTGAC TGTGGTTGTC AGCATGTACA AGAGCTTTTT TATCATCGTA 
GGAATGTTTC TCTTGCTGCT GTGCTATGCC TTTGCTGGAG TTGTTCTGTT TGGTACCGTA 
AAGTATGGTG AGAACATTAA CAGGCATGCC AATTTTTCCT CAGCTGGCAA AGCCATTACT 
GTATTGTTTC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA TTGTATGGTT 
CAACCTCCAT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGCAATTAT 
GCAGGGGCAC TTATGTACTT CTGCTCATTC TATGTCATCA TTGCCTACAT CATGCTGAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCTTTGTTTT ATTCCACTGA AGAGGACCAG 
CTTTTGAGTT ACAATGATCT TCGCCATTTT CAAATCATAT GGAACATGGT AGATGATAAA 
AGAGAGGGTG TGATCCCCAC TTTCCGAGTG AAGTTCCTGC TACGGCTGCT GCGTGGGAGG 
CTGGAAGTGG ATCTTGATAA AGACAAGCTC CTGTTTAAGC ATATGTGCTA TGAGATGGAG 
AGGCTGCACA ATGGTGGTGA TGTCACCTTC CATGATGTCT TAAGCATGCT CTCCTATCGC 
TCTGTAGACA TCAGAAAAAG CCTGCAGCTA GAGGAGCTGC TGGCAAGGGA GCAGCTAGAA 
TACACCATAG AGGAGGAGGT GGCTAAGCAG ACTATTCGCA TGTGGCTGAA GAAGTGCTTG 
AAACGCATCC GGGCTAAACA ACAGCAGTCG TGCAGCATCA TCCACAGCCT GAGAGAGAGC 
CAGGAGCAAG AGCGGAGCCG GCTATTCCTG AATCCTCCCA GCATTGAGAC CACCCAGCCA 
AGTGAGGACA GCAACGCCAA CAGCCAGGAC CACAGTATGC AACCTGAGAC AAGCAGTCAG 
CAGCAGCTCT TAAGCCCTAC TCTGTCAGAC AGAGGAGGAA GCCGACAGGA TGCAGCAGAT 
ACTGGAAAAC CCCAAAGGAA GATTGGGCAA TGGCGTCTGC CCTCAGCCCC CAAACCAATA 
AGCCATTCTG TATCTTCGGT TAACCTACGG TTTGGAGGAA GGACAACGAT GAAGACTGTG 
GTGTGCAAGA TGAACCCCAT GCCAGACACA GCTTCCTGTG GCTCTGAAGT TAAAAAGTGG 
TGGACCAGAC AGCTGACCGT GGAGAGTGAC GAGAGTGGAG ATGACCTCCT GGATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_017316062.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171551.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X1, mRNA.

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CloneID	OMu74946
Clone ID Related Accession (Same CDS sequence)	XM_017316062.1 , XM_017316062.2
Accession Version	XM_017316062.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATTGCAT TGCTGGTTCT GGCCCAGTCT 
GTGTTGCTAT CTGTCAAGTG GGATGTTGAC GATCCTGTGA CGGTTCCTTT GGCAACAATG 
TCAGTTGTCT TCACCTTCAT CTTTGTCTTA GAGGTTACAA TGAAGATTAT AGCAATGTCA 
CCAGCTGGAT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCTTGGTGAC ATCTCTTGGT 
GTTGTGTGGG TGGTGCTCCA TTTTGCTCTG CTGAATGCAT ACACCTACAT GATGGGAGCC 
TGCGTGATTG TCTTTAGATT TTTCTCCATC TGTGGGAAGC ATGTGACATT AAAGATGCTC 
CTTCTGACTG TGGTTGTCAG CATGTACAAG AGCTTTTTTA TCATCGTAGG AATGTTTCTC 
TTGCTGCTGT GCTATGCCTT TGCTGGAGTT GTTCTGTTTG GTACCGTAAA GTATGGTGAG 
AACATTAACA GGCATGCCAA TTTTTCCTCA GCTGGCAAAG CCATTACTGT ATTGTTTCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGATT GTATGGTTCA ACCTCCATTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GCAATTATGC AGGGGCACTT 
ATGTACTTCT GCTCATTCTA TGTCATCATT GCCTACATCA TGCTGAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC TTTGTTTTAT TCCACTGAAG AGGACCAGCT TTTGAGTTAC 
AATGATCTTC GCCATTTTCA AATCATATGG AACATGGTAG ATGATAAAAG AGAGGGTGTG 
ATCCCCACTT TCCGAGTGAA GTTCCTGCTA CGGCTGCTGC GTGGGAGGCT GGAAGTGGAT 
CTTGATAAAG ACAAGCTCCT GTTTAAGCAT ATGTGCTATG AGATGGAGAG GCTGCACAAT 
GGTGGTGATG TCACCTTCCA TGATGTCTTA AGCATGCTCT CCTATCGCTC TGTAGACATC 
AGAAAAAGCC TGCAGCTAGA GGAGCTGCTG GCAAGGGAGC AGCTAGAATA CACCATAGAG 
GAGGAGGTGG CTAAGCAGAC TATTCGCATG TGGCTGAAGA AGTGCTTGAA ACGCATCCGG 
GCTAAACAAC AGCAGTCGTG CAGCATCATC CACAGCCTGA GAGAGAGCCA GGAGCAAGAG 
CGGAGCCGGC TATTCCTGAA TCCTCCCAGC ATTGAGACCA CCCAGCCAAG TGAGGACAGC 
AACGCCAACA GCCAGGACCA CAGTATGCAA CCTGAGACAA GCAGTCAGCA GCAGCTCTTA 
AGCCCTACTC TGTCAGACAG AGGAGGAAGC CGACAGGATG CAGCAGATAC TGGAAAACCC 
CAAAGGAAGA TTGGGCAATG GCGTCTGCCC TCAGCTGCCA TCGCTGAGAA GACGGCACCT 
GTTAACAAAA TGAGCGGTTA CAGCCAAGAT CCAGAAAGAG AGGAGGAAGA GGTTTCCAAG 
GGCTTCCTGG CCCCCAAACC AATAAGCCAT TCTGTATCTT CGGTTAACCT ACGGTTTGGA 
GGAAGGACAA CGATGAAGAC TGTGGTGTGC AAGATGAACC CCATGCCAGA CACAGCTTCC 
TGTGGCTCTG AAGTTAAAAA GTGGTGGACC AGACAGCTGA CCGTGGAGAG TGACGAGAGT 
GGAGATGACC TCCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171551.1
CDS	87..5450
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAAIAEKTAPVNKMSGYSQDPEREEEEVSKGFLAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_017316062.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316062.1

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATTGCAT TGCTGGTTCT GGCCCAGTCT 
GTGTTGCTAT CTGTCAAGTG GGATGTTGAC GATCCTGTGA CGGTTCCTTT GGCAACAATG 
TCAGTTGTCT TCACCTTCAT CTTTGTCTTA GAGGTTACAA TGAAGATTAT AGCAATGTCA 
CCAGCTGGAT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCTTGGTGAC ATCTCTTGGT 
GTTGTGTGGG TGGTGCTCCA TTTTGCTCTG CTGAATGCAT ACACCTACAT GATGGGAGCC 
TGCGTGATTG TCTTTAGATT TTTCTCCATC TGTGGGAAGC ATGTGACATT AAAGATGCTC 
CTTCTGACTG TGGTTGTCAG CATGTACAAG AGCTTTTTTA TCATCGTAGG AATGTTTCTC 
TTGCTGCTGT GCTATGCCTT TGCTGGAGTT GTTCTGTTTG GTACCGTAAA GTATGGTGAG 
AACATTAACA GGCATGCCAA TTTTTCCTCA GCTGGCAAAG CCATTACTGT ATTGTTTCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGATT GTATGGTTCA ACCTCCATTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GCAATTATGC AGGGGCACTT 
ATGTACTTCT GCTCATTCTA TGTCATCATT GCCTACATCA TGCTGAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC TTTGTTTTAT TCCACTGAAG AGGACCAGCT TTTGAGTTAC 
AATGATCTTC GCCATTTTCA AATCATATGG AACATGGTAG ATGATAAAAG AGAGGGTGTG 
ATCCCCACTT TCCGAGTGAA GTTCCTGCTA CGGCTGCTGC GTGGGAGGCT GGAAGTGGAT 
CTTGATAAAG ACAAGCTCCT GTTTAAGCAT ATGTGCTATG AGATGGAGAG GCTGCACAAT 
GGTGGTGATG TCACCTTCCA TGATGTCTTA AGCATGCTCT CCTATCGCTC TGTAGACATC 
AGAAAAAGCC TGCAGCTAGA GGAGCTGCTG GCAAGGGAGC AGCTAGAATA CACCATAGAG 
GAGGAGGTGG CTAAGCAGAC TATTCGCATG TGGCTGAAGA AGTGCTTGAA ACGCATCCGG 
GCTAAACAAC AGCAGTCGTG CAGCATCATC CACAGCCTGA GAGAGAGCCA GGAGCAAGAG 
CGGAGCCGGC TATTCCTGAA TCCTCCCAGC ATTGAGACCA CCCAGCCAAG TGAGGACAGC 
AACGCCAACA GCCAGGACCA CAGTATGCAA CCTGAGACAA GCAGTCAGCA GCAGCTCTTA 
AGCCCTACTC TGTCAGACAG AGGAGGAAGC CGACAGGATG CAGCAGATAC TGGAAAACCC 
CAAAGGAAGA TTGGGCAATG GCGTCTGCCC TCAGCTGCCA TCGCTGAGAA GACGGCACCT 
GTTAACAAAA TGAGCGGTTA CAGCCAAGAT CCAGAAAGAG AGGAGGAAGA GGTTTCCAAG 
GGCTTCCTGG CCCCCAAACC AATAAGCCAT TCTGTATCTT CGGTTAACCT ACGGTTTGGA 
GGAAGGACAA CGATGAAGAC TGTGGTGTGC AAGATGAACC CCATGCCAGA CACAGCTTCC 
TGTGGCTCTG AAGTTAAAAA GTGGTGGACC AGACAGCTGA CCGTGGAGAG TGACGAGAGT 
GGAGATGACC TCCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_011245094.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243396.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X2, mRNA.

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CloneID	OMu74947
Clone ID Related Accession (Same CDS sequence)	XM_011245094.3 , XM_011245094.2
Accession Version	XM_011245094.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GAAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTTACTGA CTTTGGTCCT 
GATGAATCTC TGTCAGACAA TGCTGACATA CTCTGGATTA ATAAGCCATG GGTGCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTGT GCATGAACAC ACCTATGACC 
TTTGAGCACT ATCCTCCTCT TCAGTATGTG ACCTTCACCT TGGACACTTT ATTGATGTTC 
CTCTACACTG CAGAAATGAT AGCAAAGATG CACATACGGG GAATTGTCAA GGGTGATAGC 
TCCTATGTGA AGGATCGCTG GTGTGTTTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCTCTTGTGT TACAGGTGTT TGAAATAGCT GACATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGCTGCGGA TCCCACGGCC ACTCATTATG ATCCGGGCTT TCAGGATTTA TTTCCGATTC 
GAACTGCCAA GGACAAGAAT TACAAACATT TTAAAGCGGT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTCCTTCTT CTGTATGGGA TTTTAGGAGT TCAAATGTTT 
GGAACATTTA CCTACCACTG TGTAGTCAAT GACACAAAGC CAGGAAATGT AACCTGGAAT 
AGCTTAGCTA TTCCAGATAC GCACTGCTCC CCAGAGCTAG AAGAAGGCTA TCAATGCCCG 
CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG CACGAGTATA TTCACAGTCT ATGAGGCTTC ATCTCAGGAA 
GGCTGGGTAT TCCTCATGTA CAGAGCAATC GACAGCTTTC CCCGCTGGCG CTCCTACTTC 
TACTTCATCA CACTGATTTT CTTCCTTGCT TGGCTTGTCA AGAATGTGTT TATTGCTGTC 
ATCATTGAGA CATTTGCAGA AATCAGAGTA CAATTTCAAC AAATGTGGGG AACTCGGAGC 
AGCACAACTT CTACAGCCAC CACACAGATG TTCCATGAAG ATGCCGCTGG TGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGTGCCC CAGCCTGCCT ACAGAAAATG 
ATGCGGTCAT CGGTTTTCCA CATGTTTATC CTGAGCATGG TGACTGTGGA TGTAATAGTT 
GCTGCCAGCA ATTACTACAA GGGAGAGAAC TTCAGAAGGC AGTATGATGA ATTCTACCTT 
GCAGAGGTGG CTTTTACAGT CCTTTTCGAC CTGGAAGCAC TCCTGAAGAT ATGGTGTTTG 
GGGTTTACTG GCTACATCAG CTCATCTCTC CACAAGTTTG AACTATTGCT TGTTATTGGG 
ACGACGCTTC ATGTGTACCC AGATCTTTAT CATTCTCAGT TCACATACTT CCAGGTTCTG 
CGGGTAGTCC GGCTTATTAA GATTTCCCCA GCATTGGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGGA AAAAACTTGG AAGCTTGGTG GTGTTCACTG CCAGCCTCCT GATAGTTATG 
TCAGCCATCA GTTTGCAGAT GTTCTGTTTT GTCGAAGAAC TGGACAGATT CACCACTTTT 
CCAAGGGCAT TTATGTCTAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGATGTGATG 
GATCAGACTC TGAATGCTGT GGGGCATATG TGGGCACCAC TGGTTGCCAT CTATTTCATC 
CTCTATCATC TCTTTGCAAC TTTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA TCTAAAGAAG CTCAAACAAC TAAAGCAAAG TGAAGCGAAC 
GCGGACACCA AAGAAAAACT CCCTTTGCGC TTGAGAATCT TCGAAAAATT TCCAAACAGA 
CCGCAAATGG TGAAAATCTC AAAACTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGCTTCATGA AGCAGTTCAT TGACCGCCAG CAACAGGACA CCTGCTGTCT CTTCAGAATC 
CTCCCCTCTA CCTCTTCCTC ATCATGTGAC AACCCCAAGA AGCCCACAGC TGAAGACAAC 
AAATACATTG ATCAAAAACT CCGCAAGTCT GTTTTCAGCA TAAGGGCGAG GAACCTACTG 
GAAAAGGAGA CTGCAGTCAC AAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC AGCAAAGGAG AGGTCGATCC TCAGCGTGCA GCATCACATC 
CGCCAGGAGC GCAGGTCACT AAGACATGGA TCCAACAGCC AGAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTTTAACTCA AGACCATTCA AATACAGTGC GCTACAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAAAT GATTCAGGAG AAGAAGGAAC AAGCCGAGAT GAAAAGGAAG 
GTGCAAGAAG AGGAACTGCG GGAGAACCAC CCATACTTTG ACAAGCCTCT CTTCATCGTG 
GGGCGGGAGC ACAGGTTCAG AAACTTCTGC CGCGTGGTGG TTCGAGCACG CTTCAATGCA 
TCCAAAACAG ACCCTGTCAC GGGAGCTGTG AAAAATACAA AGTACCACCA ACTTTATGAT 
TTGCTGGGAC TTGTCACCTA CCTGGACTGG GTCATGATCA CTGTAACCAT CTGCTCTTGC 
ATTTCTATGA TGTTCGAATC CCCCTTCCGA AGAGTCATGC ATGCACCCAC TTTACAGATC 
GCAGAATATG TGTTTGTAAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTGTTTT TCACTCCAAC TGCTGTCATC AGGGACTTTG GTGGCGTCAT GGACATATTT 
ATCTATCTTG TGAGTTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCCGAGTCA 
GGAGCCCAGC TACTGATGGT TCTTCGGTGC CTAAGACCTC TTCGGATATT CAAACTTGTG 
CCCCAAATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGTT TCAAGGAAAT ATTTTTGGTC 
TCCATTCTGT TGTTGACGTT AATGCTTGTT TTTGCAAGCT TTGGGGTTCA GCTCTTTGCT 
GGAAAGTTAG CCAAGTGCAA CGACCCCAAC ATTATCAGAA GGGAAGACTG TAATGGCATC 
TTCAGAATTA ATGTAAGTGT GTCCAAGAAC TTAAATTTAA AACTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTCGACAAT 
GTGGGAAATG CCATGCTGGC ATTATTTGAA GTTCTGTCCT TGAAAGGCTG GGTAGAAGTG 
AGGGATGTCA TTATTCATCG TGTGGGGCCG ATCCACGGAA TCTATATTCA TGTTTTCGTA 
TTCCTGGGTT GCATGATCGG ACTGACGCTT TTTGTCGGTG TAGTTATTGC TAACTTCAAT 
GAAAACAAGG GGACAGCCCT GCTGACGGTA GATCAGAGAC GATGGGAAGA TCTCAAGAGC 
AGACTGAAGA TCGCACAGCC TCTTCATCTC CCTCCTCGGC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATTGC ATTGCTGGTT 
CTGGCCCAGT CTGTGTTGCT ATCTGTCAAG TGGGATGTTG ACGATCCTGT GACGGTTCCT 
TTGGCAACAA TGTCAGTTGT CTTCACCTTC ATCTTTGTCT TAGAGGTTAC AATGAAGATT 
ATAGCAATGT CACCAGCTGG ATTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCTTGGTG 
ACATCTCTTG GTGTTGTGTG GGTGGTGCTC CATTTTGCTC TGCTGAATGC ATACACCTAC 
ATGATGGGAG CCTGCGTGAT TGTCTTTAGA TTTTTCTCCA TCTGTGGGAA GCATGTGACA 
TTAAAGATGC TCCTTCTGAC TGTGGTTGTC AGCATGTACA AGAGCTTTTT TATCATCGTA 
GGAATGTTTC TCTTGCTGCT GTGCTATGCC TTTGCTGGAG TTGTTCTGTT TGGTACCGTA 
AAGTATGGTG AGAACATTAA CAGGCATGCC AATTTTTCCT CAGCTGGCAA AGCCATTACT 
GTATTGTTTC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA TTGTATGGTT 
CAACCTCCAT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGCAATTAT 
GCAGGGGCAC TTATGTACTT CTGCTCATTC TATGTCATCA TTGCCTACAT CATGCTGAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCTTTGTTTT ATTCCACTGA AGAGGACCAG 
CTTTTGAGTT ACAATGATCT TCGCCATTTT CAAATCATAT GGAACATGGT AGATGATAAA 
AGAGAGGGTG TGATCCCCAC TTTCCGAGTG AAGTTCCTGC TACGGCTGCT GCGTGGGAGG 
CTGGAAGTGG ATCTTGATAA AGACAAGCTC CTGTTTAAGC ATATGTGCTA TGAGATGGAG 
AGGCTGCACA ATGGTGGTGA TGTCACCTTC CATGATGTCT TAAGCATGCT CTCCTATCGC 
TCTGTAGACA TCAGAAAAAG CCTGCAGCTA GAGGAGCTGC TGGCAAGGGA GCAGCTAGAA 
TACACCATAG AGGAGGAGGT GGCTAAGCAG ACTATTCGCA TGTGGCTGAA GAAGTGCTTG 
AAACGCATCC GGGCTAAACA ACAGCAGTCG TGCAGCATCA TCCACAGCCT GAGAGAGAGC 
CAGGAGCAAG AGCGGAGCCG GCTATTCCTG AATCCTCCCA GCATTGAGAC CACCCAGCCA 
AGTGAGGACA GCAACGCCAA CAGCCAGGAC CACAGTATGC AACCTGAGAC AAGCAGTCAG 
CAGCAGCTCT TAAGCCCTAC TCTGTCAGAC AGAGGAGGAA GCCGACAGGA TGCAGCAGAT 
ACTGGAAAAC CCCAAAGGAA GATTGGGCAA TGGCGTCTGC CCTCAGCTGC CATCGCTGAG 
AAGACGGCAC CTGTTAACAA AATGAGCGGT TACAGCCAAG ATCCAGAAAG AGAGGAGGAA 
GAGGTTTCCA AGGGCTTCCT GGCCCCCAAA CCAATAAGCC ATTCTGTATC TTCGGTTAAC 
CTACGGTTTG GAGGAAGGAC AACGATGAAG ACTGTGGTGT GCAAGATGAA CCCCATGCCA 
GACACAGCTT CCTGTGGCTC TGAAGTTAAA AAGTGGTGGA CCAGACAGCT GACCGTGGAG 
AGTGACGAGA GTGGAGATGA CCTCCTGGAT ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243396.1
CDS	595..5910
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAAIAEKTAPVNKMSGYSQDPEREEEEVSKGFLAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_011245094.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245094.2

ORF Insert Sequence:

ATGCTCAAAA GAAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTTACTGA CTTTGGTCCT 
GATGAATCTC TGTCAGACAA TGCTGACATA CTCTGGATTA ATAAGCCATG GGTGCACTCT 
CTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATCTCAGTGT GCATGAACAC ACCTATGACC 
TTTGAGCACT ATCCTCCTCT TCAGTATGTG ACCTTCACCT TGGACACTTT ATTGATGTTC 
CTCTACACTG CAGAAATGAT AGCAAAGATG CACATACGGG GAATTGTCAA GGGTGATAGC 
TCCTATGTGA AGGATCGCTG GTGTGTTTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCTCTTGTGT TACAGGTGTT TGAAATAGCT GACATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGCTGCGGA TCCCACGGCC ACTCATTATG ATCCGGGCTT TCAGGATTTA TTTCCGATTC 
GAACTGCCAA GGACAAGAAT TACAAACATT TTAAAGCGGT CAGGAGAACA AATATGGAGT 
GTTTCCATTT TCCTCCTTTT CTTCCTTCTT CTGTATGGGA TTTTAGGAGT TCAAATGTTT 
GGAACATTTA CCTACCACTG TGTAGTCAAT GACACAAAGC CAGGAAATGT AACCTGGAAT 
AGCTTAGCTA TTCCAGATAC GCACTGCTCC CCAGAGCTAG AAGAAGGCTA TCAATGCCCG 
CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG CACGAGTATA TTCACAGTCT ATGAGGCTTC ATCTCAGGAA 
GGCTGGGTAT TCCTCATGTA CAGAGCAATC GACAGCTTTC CCCGCTGGCG CTCCTACTTC 
TACTTCATCA CACTGATTTT CTTCCTTGCT TGGCTTGTCA AGAATGTGTT TATTGCTGTC 
ATCATTGAGA CATTTGCAGA AATCAGAGTA CAATTTCAAC AAATGTGGGG AACTCGGAGC 
AGCACAACTT CTACAGCCAC CACACAGATG TTCCATGAAG ATGCCGCTGG TGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGTGCCC CAGCCTGCCT ACAGAAAATG 
ATGCGGTCAT CGGTTTTCCA CATGTTTATC CTGAGCATGG TGACTGTGGA TGTAATAGTT 
GCTGCCAGCA ATTACTACAA GGGAGAGAAC TTCAGAAGGC AGTATGATGA ATTCTACCTT 
GCAGAGGTGG CTTTTACAGT CCTTTTCGAC CTGGAAGCAC TCCTGAAGAT ATGGTGTTTG 
GGGTTTACTG GCTACATCAG CTCATCTCTC CACAAGTTTG AACTATTGCT TGTTATTGGG 
ACGACGCTTC ATGTGTACCC AGATCTTTAT CATTCTCAGT TCACATACTT CCAGGTTCTG 
CGGGTAGTCC GGCTTATTAA GATTTCCCCA GCATTGGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGGA AAAAACTTGG AAGCTTGGTG GTGTTCACTG CCAGCCTCCT GATAGTTATG 
TCAGCCATCA GTTTGCAGAT GTTCTGTTTT GTCGAAGAAC TGGACAGATT CACCACTTTT 
CCAAGGGCAT TTATGTCTAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGATGTGATG 
GATCAGACTC TGAATGCTGT GGGGCATATG TGGGCACCAC TGGTTGCCAT CTATTTCATC 
CTCTATCATC TCTTTGCAAC TTTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA TCTAAAGAAG CTCAAACAAC TAAAGCAAAG TGAAGCGAAC 
GCGGACACCA AAGAAAAACT CCCTTTGCGC TTGAGAATCT TCGAAAAATT TCCAAACAGA 
CCGCAAATGG TGAAAATCTC AAAACTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA 
AGCTTCATGA AGCAGTTCAT TGACCGCCAG CAACAGGACA CCTGCTGTCT CTTCAGAATC 
CTCCCCTCTA CCTCTTCCTC ATCATGTGAC AACCCCAAGA AGCCCACAGC TGAAGACAAC 
AAATACATTG ATCAAAAACT CCGCAAGTCT GTTTTCAGCA TAAGGGCGAG GAACCTACTG 
GAAAAGGAGA CTGCAGTCAC AAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC AGCAAAGGAG AGGTCGATCC TCAGCGTGCA GCATCACATC 
CGCCAGGAGC GCAGGTCACT AAGACATGGA TCCAACAGCC AGAGGATCAG CAGGGGGAAA 
TCTCTTGAAA CTTTAACTCA AGACCATTCA AATACAGTGC GCTACAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAAAT GATTCAGGAG AAGAAGGAAC AAGCCGAGAT GAAAAGGAAG 
GTGCAAGAAG AGGAACTGCG GGAGAACCAC CCATACTTTG ACAAGCCTCT CTTCATCGTG 
GGGCGGGAGC ACAGGTTCAG AAACTTCTGC CGCGTGGTGG TTCGAGCACG CTTCAATGCA 
TCCAAAACAG ACCCTGTCAC GGGAGCTGTG AAAAATACAA AGTACCACCA ACTTTATGAT 
TTGCTGGGAC TTGTCACCTA CCTGGACTGG GTCATGATCA CTGTAACCAT CTGCTCTTGC 
ATTTCTATGA TGTTCGAATC CCCCTTCCGA AGAGTCATGC ATGCACCCAC TTTACAGATC 
GCAGAATATG TGTTTGTAAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTGTTTT TCACTCCAAC TGCTGTCATC AGGGACTTTG GTGGCGTCAT GGACATATTT 
ATCTATCTTG TGAGTTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCCGAGTCA 
GGAGCCCAGC TACTGATGGT TCTTCGGTGC CTAAGACCTC TTCGGATATT CAAACTTGTG 
CCCCAAATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGTT TCAAGGAAAT ATTTTTGGTC 
TCCATTCTGT TGTTGACGTT AATGCTTGTT TTTGCAAGCT TTGGGGTTCA GCTCTTTGCT 
GGAAAGTTAG CCAAGTGCAA CGACCCCAAC ATTATCAGAA GGGAAGACTG TAATGGCATC 
TTCAGAATTA ATGTAAGTGT GTCCAAGAAC TTAAATTTAA AACTAAGACC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTC GGAACTTTAA CTTCGACAAT 
GTGGGAAATG CCATGCTGGC ATTATTTGAA GTTCTGTCCT TGAAAGGCTG GGTAGAAGTG 
AGGGATGTCA TTATTCATCG TGTGGGGCCG ATCCACGGAA TCTATATTCA TGTTTTCGTA 
TTCCTGGGTT GCATGATCGG ACTGACGCTT TTTGTCGGTG TAGTTATTGC TAACTTCAAT 
GAAAACAAGG GGACAGCCCT GCTGACGGTA GATCAGAGAC GATGGGAAGA TCTCAAGAGC 
AGACTGAAGA TCGCACAGCC TCTTCATCTC CCTCCTCGGC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATTGC ATTGCTGGTT 
CTGGCCCAGT CTGTGTTGCT ATCTGTCAAG TGGGATGTTG ACGATCCTGT GACGGTTCCT 
TTGGCAACAA TGTCAGTTGT CTTCACCTTC ATCTTTGTCT TAGAGGTTAC AATGAAGATT 
ATAGCAATGT CACCAGCTGG ATTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCTTGGTG 
ACATCTCTTG GTGTTGTGTG GGTGGTGCTC CATTTTGCTC TGCTGAATGC ATACACCTAC 
ATGATGGGAG CCTGCGTGAT TGTCTTTAGA TTTTTCTCCA TCTGTGGGAA GCATGTGACA 
TTAAAGATGC TCCTTCTGAC TGTGGTTGTC AGCATGTACA AGAGCTTTTT TATCATCGTA 
GGAATGTTTC TCTTGCTGCT GTGCTATGCC TTTGCTGGAG TTGTTCTGTT TGGTACCGTA 
AAGTATGGTG AGAACATTAA CAGGCATGCC AATTTTTCCT CAGCTGGCAA AGCCATTACT 
GTATTGTTTC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA TTGTATGGTT 
CAACCTCCAT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGCAATTAT 
GCAGGGGCAC TTATGTACTT CTGCTCATTC TATGTCATCA TTGCCTACAT CATGCTGAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCTTTGTTTT ATTCCACTGA AGAGGACCAG 
CTTTTGAGTT ACAATGATCT TCGCCATTTT CAAATCATAT GGAACATGGT AGATGATAAA 
AGAGAGGGTG TGATCCCCAC TTTCCGAGTG AAGTTCCTGC TACGGCTGCT GCGTGGGAGG 
CTGGAAGTGG ATCTTGATAA AGACAAGCTC CTGTTTAAGC ATATGTGCTA TGAGATGGAG 
AGGCTGCACA ATGGTGGTGA TGTCACCTTC CATGATGTCT TAAGCATGCT CTCCTATCGC 
TCTGTAGACA TCAGAAAAAG CCTGCAGCTA GAGGAGCTGC TGGCAAGGGA GCAGCTAGAA 
TACACCATAG AGGAGGAGGT GGCTAAGCAG ACTATTCGCA TGTGGCTGAA GAAGTGCTTG 
AAACGCATCC GGGCTAAACA ACAGCAGTCG TGCAGCATCA TCCACAGCCT GAGAGAGAGC 
CAGGAGCAAG AGCGGAGCCG GCTATTCCTG AATCCTCCCA GCATTGAGAC CACCCAGCCA 
AGTGAGGACA GCAACGCCAA CAGCCAGGAC CACAGTATGC AACCTGAGAC AAGCAGTCAG 
CAGCAGCTCT TAAGCCCTAC TCTGTCAGAC AGAGGAGGAA GCCGACAGGA TGCAGCAGAT 
ACTGGAAAAC CCCAAAGGAA GATTGGGCAA TGGCGTCTGC CCTCAGCTGC CATCGCTGAG 
AAGACGGCAC CTGTTAACAA AATGAGCGGT TACAGCCAAG ATCCAGAAAG AGAGGAGGAA 
GAGGTTTCCA AGGGCTTCCT GGCCCCCAAA CCAATAAGCC ATTCTGTATC TTCGGTTAAC 
CTACGGTTTG GAGGAAGGAC AACGATGAAG ACTGTGGTGT GCAAGATGAA CCCCATGCCA 
GACACAGCTT CCTGTGGCTC TGAAGTTAAA AAGTGGTGGA CCAGACAGCT GACCGTGGAG 
AGTGACGAGA GTGGAGATGA CCTCCTGGAT ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_017316063.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171552.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X3, mRNA.

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CloneID	OMu74948
Clone ID Related Accession (Same CDS sequence)	XM_017316063.1 , XM_017316063.2
Accession Version	XM_017316063.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGCTAAAGCA AAGTGAAGCG AACGCGGACA CCAAAGAAAA ACTCCCTTTG 
CGCTTGAGAA TCTTCGAAAA ATTTCCAAAC AGACCGCAAA TGGTGAAAAT CTCAAAACTT 
CCTTCAGATT TTACAGTTCC TAAAATCAGG GAAAGCTTCA TGAAGCAGTT CATTGACCGC 
CAGCAACAGG ACACCTGCTG TCTCTTCAGA ATCCTCCCCT CTACCTCTTC CTCATCATGT 
GACAACCCCA AGAAGCCCAC AGCTGAAGAC AACAAATACA TTGATCAAAA ACTCCGCAAG 
TCTGTTTTCA GCATAAGGGC GAGGAACCTA CTGGAAAAGG AGACTGCAGT CACAAAAATC 
TTAAGAGCTT GCACTCGACA GCGCATGCTG AGCGGATCAT TCGAGGGGCA GCCAGCAAAG 
GAGAGGTCGA TCCTCAGCGT GCAGCATCAC ATCCGCCAGG AGCGCAGGTC ACTAAGACAT 
GGATCCAACA GCCAGAGGAT CAGCAGGGGG AAATCTCTTG AAACTTTAAC TCAAGACCAT 
TCAAATACAG TGCGCTACAG AAATGCACAA AGAGAAGACA GTGAAATAAA AATGATTCAG 
GAGAAGAAGG AACAAGCCGA GATGAAAAGG AAGGTGCAAG AAGAGGAACT GCGGGAGAAC 
CACCCATACT TTGACAAGCC TCTCTTCATC GTGGGGCGGG AGCACAGGTT CAGAAACTTC 
TGCCGCGTGG TGGTTCGAGC ACGCTTCAAT GCATCCAAAA CAGACCCTGT CACGGGAGCT 
GTGAAAAATA CAAAGTACCA CCAACTTTAT GATTTGCTGG GACTTGTCAC CTACCTGGAC 
TGGGTCATGA TCACTGTAAC CATCTGCTCT TGCATTTCTA TGATGTTCGA ATCCCCCTTC 
CGAAGAGTCA TGCATGCACC CACTTTACAG ATCGCAGAAT ATGTGTTTGT AATATTCATG 
AGCATTGAGC TTAATCTGAA GATTATGGCA GATGGCTTGT TTTTCACTCC AACTGCTGTC 
ATCAGGGACT TTGGTGGCGT CATGGACATA TTTATCTATC TTGTGAGTTT GATATTTCTT 
TGTTGGATGC CTCAAAATGT ACCTGCCGAG TCAGGAGCCC AGCTACTGAT GGTTCTTCGG 
TGCCTAAGAC CTCTTCGGAT ATTCAAACTT GTGCCCCAAA TGAGGAAAGT TGTTCGAGAA 
CTTTTCAGTG GTTTCAAGGA AATATTTTTG GTCTCCATTC TGTTGTTGAC GTTAATGCTT 
GTTTTTGCAA GCTTTGGGGT TCAGCTCTTT GCTGGAAAGT TAGCCAAGTG CAACGACCCC 
AACATTATCA GAAGGGAAGA CTGTAATGGC ATCTTCAGAA TTAATGTAAG TGTGTCCAAG 
AACTTAAATT TAAAACTAAG ACCTGGAGAG AAAAAACCTG GATTTTGGGT GCCCCGTGTT 
TGGGCAAATC CTCGGAACTT TAACTTCGAC AATGTGGGAA ATGCCATGCT GGCATTATTT 
GAAGTTCTGT CCTTGAAAGG CTGGGTAGAA GTGAGGGATG TCATTATTCA TCGTGTGGGG 
CCGATCCACG GAATCTATAT TCATGTTTTC GTATTCCTGG GTTGCATGAT CGGACTGACG 
CTTTTTGTCG GTGTAGTTAT TGCTAACTTC AATGAAAACA AGGGGACAGC CCTGCTGACG 
GTAGATCAGA GACGATGGGA AGATCTCAAG AGCAGACTGA AGATCGCACA GCCTCTTCAT 
CTCCCTCCTC GGCCGGATAA TGATGGTTTT AGAGCTAAAA TGTATGACAT AACCCAGCAT 
CCATTTTTTA AGAGGACAAT TGCATTGCTG GTTCTGGCCC AGTCTGTGTT GCTATCTGTC 
AAGTGGGATG TTGACGATCC TGTGACGGTT CCTTTGGCAA CAATGTCAGT TGTCTTCACC 
TTCATCTTTG TCTTAGAGGT TACAATGAAG ATTATAGCAA TGTCACCAGC TGGATTCTGG 
CAAAGCAGAA GAAACCGATA TGATCTCTTG GTGACATCTC TTGGTGTTGT GTGGGTGGTG 
CTCCATTTTG CTCTGCTGAA TGCATACACC TACATGATGG GAGCCTGCGT GATTGTCTTT 
AGATTTTTCT CCATCTGTGG GAAGCATGTG ACATTAAAGA TGCTCCTTCT GACTGTGGTT 
GTCAGCATGT ACAAGAGCTT TTTTATCATC GTAGGAATGT TTCTCTTGCT GCTGTGCTAT 
GCCTTTGCTG GAGTTGTTCT GTTTGGTACC GTAAAGTATG GTGAGAACAT TAACAGGCAT 
GCCAATTTTT CCTCAGCTGG CAAAGCCATT ACTGTATTGT TTCGAATTGT CACAGGTGAA 
GACTGGAACA AGATTATGCA TGATTGTATG GTTCAACCTC CATTTTGTAC TCCAGATGAA 
TTTACATACT GGGCAACAGA CTGTGGCAAT TATGCAGGGG CACTTATGTA CTTCTGCTCA 
TTCTATGTCA TCATTGCCTA CATCATGCTG AATCTGCTTG TAGCCATAAT TGTGGAGAAT 
TTCTCTTTGT TTTATTCCAC TGAAGAGGAC CAGCTTTTGA GTTACAATGA TCTTCGCCAT 
TTTCAAATCA TATGGAACAT GGTAGATGAT AAAAGAGAGG GTGTGATCCC CACTTTCCGA 
GTGAAGTTCC TGCTACGGCT GCTGCGTGGG AGGCTGGAAG TGGATCTTGA TAAAGACAAG 
CTCCTGTTTA AGCATATGTG CTATGAGATG GAGAGGCTGC ACAATGGTGG TGATGTCACC 
TTCCATGATG TCTTAAGCAT GCTCTCCTAT CGCTCTGTAG ACATCAGAAA AAGCCTGCAG 
CTAGAGGAGC TGCTGGCAAG GGAGCAGCTA GAATACACCA TAGAGGAGGA GGTGGCTAAG 
CAGACTATTC GCATGTGGCT GAAGAAGTGC TTGAAACGCA TCCGGGCTAA ACAACAGCAG 
TCGTGCAGCA TCATCCACAG CCTGAGAGAG AGCCAGGAGC AAGAGCGGAG CCGGCTATTC 
CTGAATCCTC CCAGCATTGA GACCACCCAG CCAAGTGAGG ACAGCAACGC CAACAGCCAG 
GACCACAGTA TGCAACCTGA GACAAGCAGT CAGCAGCAGC TCTTAAGCCC TACTCTGTCA 
GACAGAGGAG GAAGCCGACA GGATGCAGCA GATACTGGAA AACCCCAAAG GAAGATTGGG 
CAATGGCGTC TGCCCTCAGC TGCCATCGCT GAGAAGACGG CACCTGTTAA CAAAATGAGC 
GGTTACAGCC AAGATCCAGA AAGAGAGGAG GAAGAGGTTT CCAAGGGCTT CCTGGCCCCC 
AAACCAATAA GCCATTCTGT ATCTTCGGTT AACCTACGGT TTGGAGGAAG GACAACGATG 
AAGACTGTGG TGTGCAAGAT GAACCCCATG CCAGACACAG CTTCCTGTGG CTCTGAAGTT 
AAAAAGTGGT GGACCAGACA GCTGACCGTG GAGAGTGACG AGAGTGGAGA TGACCTCCTG 
GATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171552.1
CDS	87..5375
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAAIAEKTAPVNKMSGYSQDPEREEEEVSKGFLAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_017316063.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316063.1

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGCTAAAGCA AAGTGAAGCG AACGCGGACA CCAAAGAAAA ACTCCCTTTG 
CGCTTGAGAA TCTTCGAAAA ATTTCCAAAC AGACCGCAAA TGGTGAAAAT CTCAAAACTT 
CCTTCAGATT TTACAGTTCC TAAAATCAGG GAAAGCTTCA TGAAGCAGTT CATTGACCGC 
CAGCAACAGG ACACCTGCTG TCTCTTCAGA ATCCTCCCCT CTACCTCTTC CTCATCATGT 
GACAACCCCA AGAAGCCCAC AGCTGAAGAC AACAAATACA TTGATCAAAA ACTCCGCAAG 
TCTGTTTTCA GCATAAGGGC GAGGAACCTA CTGGAAAAGG AGACTGCAGT CACAAAAATC 
TTAAGAGCTT GCACTCGACA GCGCATGCTG AGCGGATCAT TCGAGGGGCA GCCAGCAAAG 
GAGAGGTCGA TCCTCAGCGT GCAGCATCAC ATCCGCCAGG AGCGCAGGTC ACTAAGACAT 
GGATCCAACA GCCAGAGGAT CAGCAGGGGG AAATCTCTTG AAACTTTAAC TCAAGACCAT 
TCAAATACAG TGCGCTACAG AAATGCACAA AGAGAAGACA GTGAAATAAA AATGATTCAG 
GAGAAGAAGG AACAAGCCGA GATGAAAAGG AAGGTGCAAG AAGAGGAACT GCGGGAGAAC 
CACCCATACT TTGACAAGCC TCTCTTCATC GTGGGGCGGG AGCACAGGTT CAGAAACTTC 
TGCCGCGTGG TGGTTCGAGC ACGCTTCAAT GCATCCAAAA CAGACCCTGT CACGGGAGCT 
GTGAAAAATA CAAAGTACCA CCAACTTTAT GATTTGCTGG GACTTGTCAC CTACCTGGAC 
TGGGTCATGA TCACTGTAAC CATCTGCTCT TGCATTTCTA TGATGTTCGA ATCCCCCTTC 
CGAAGAGTCA TGCATGCACC CACTTTACAG ATCGCAGAAT ATGTGTTTGT AATATTCATG 
AGCATTGAGC TTAATCTGAA GATTATGGCA GATGGCTTGT TTTTCACTCC AACTGCTGTC 
ATCAGGGACT TTGGTGGCGT CATGGACATA TTTATCTATC TTGTGAGTTT GATATTTCTT 
TGTTGGATGC CTCAAAATGT ACCTGCCGAG TCAGGAGCCC AGCTACTGAT GGTTCTTCGG 
TGCCTAAGAC CTCTTCGGAT ATTCAAACTT GTGCCCCAAA TGAGGAAAGT TGTTCGAGAA 
CTTTTCAGTG GTTTCAAGGA AATATTTTTG GTCTCCATTC TGTTGTTGAC GTTAATGCTT 
GTTTTTGCAA GCTTTGGGGT TCAGCTCTTT GCTGGAAAGT TAGCCAAGTG CAACGACCCC 
AACATTATCA GAAGGGAAGA CTGTAATGGC ATCTTCAGAA TTAATGTAAG TGTGTCCAAG 
AACTTAAATT TAAAACTAAG ACCTGGAGAG AAAAAACCTG GATTTTGGGT GCCCCGTGTT 
TGGGCAAATC CTCGGAACTT TAACTTCGAC AATGTGGGAA ATGCCATGCT GGCATTATTT 
GAAGTTCTGT CCTTGAAAGG CTGGGTAGAA GTGAGGGATG TCATTATTCA TCGTGTGGGG 
CCGATCCACG GAATCTATAT TCATGTTTTC GTATTCCTGG GTTGCATGAT CGGACTGACG 
CTTTTTGTCG GTGTAGTTAT TGCTAACTTC AATGAAAACA AGGGGACAGC CCTGCTGACG 
GTAGATCAGA GACGATGGGA AGATCTCAAG AGCAGACTGA AGATCGCACA GCCTCTTCAT 
CTCCCTCCTC GGCCGGATAA TGATGGTTTT AGAGCTAAAA TGTATGACAT AACCCAGCAT 
CCATTTTTTA AGAGGACAAT TGCATTGCTG GTTCTGGCCC AGTCTGTGTT GCTATCTGTC 
AAGTGGGATG TTGACGATCC TGTGACGGTT CCTTTGGCAA CAATGTCAGT TGTCTTCACC 
TTCATCTTTG TCTTAGAGGT TACAATGAAG ATTATAGCAA TGTCACCAGC TGGATTCTGG 
CAAAGCAGAA GAAACCGATA TGATCTCTTG GTGACATCTC TTGGTGTTGT GTGGGTGGTG 
CTCCATTTTG CTCTGCTGAA TGCATACACC TACATGATGG GAGCCTGCGT GATTGTCTTT 
AGATTTTTCT CCATCTGTGG GAAGCATGTG ACATTAAAGA TGCTCCTTCT GACTGTGGTT 
GTCAGCATGT ACAAGAGCTT TTTTATCATC GTAGGAATGT TTCTCTTGCT GCTGTGCTAT 
GCCTTTGCTG GAGTTGTTCT GTTTGGTACC GTAAAGTATG GTGAGAACAT TAACAGGCAT 
GCCAATTTTT CCTCAGCTGG CAAAGCCATT ACTGTATTGT TTCGAATTGT CACAGGTGAA 
GACTGGAACA AGATTATGCA TGATTGTATG GTTCAACCTC CATTTTGTAC TCCAGATGAA 
TTTACATACT GGGCAACAGA CTGTGGCAAT TATGCAGGGG CACTTATGTA CTTCTGCTCA 
TTCTATGTCA TCATTGCCTA CATCATGCTG AATCTGCTTG TAGCCATAAT TGTGGAGAAT 
TTCTCTTTGT TTTATTCCAC TGAAGAGGAC CAGCTTTTGA GTTACAATGA TCTTCGCCAT 
TTTCAAATCA TATGGAACAT GGTAGATGAT AAAAGAGAGG GTGTGATCCC CACTTTCCGA 
GTGAAGTTCC TGCTACGGCT GCTGCGTGGG AGGCTGGAAG TGGATCTTGA TAAAGACAAG 
CTCCTGTTTA AGCATATGTG CTATGAGATG GAGAGGCTGC ACAATGGTGG TGATGTCACC 
TTCCATGATG TCTTAAGCAT GCTCTCCTAT CGCTCTGTAG ACATCAGAAA AAGCCTGCAG 
CTAGAGGAGC TGCTGGCAAG GGAGCAGCTA GAATACACCA TAGAGGAGGA GGTGGCTAAG 
CAGACTATTC GCATGTGGCT GAAGAAGTGC TTGAAACGCA TCCGGGCTAA ACAACAGCAG 
TCGTGCAGCA TCATCCACAG CCTGAGAGAG AGCCAGGAGC AAGAGCGGAG CCGGCTATTC 
CTGAATCCTC CCAGCATTGA GACCACCCAG CCAAGTGAGG ACAGCAACGC CAACAGCCAG 
GACCACAGTA TGCAACCTGA GACAAGCAGT CAGCAGCAGC TCTTAAGCCC TACTCTGTCA 
GACAGAGGAG GAAGCCGACA GGATGCAGCA GATACTGGAA AACCCCAAAG GAAGATTGGG 
CAATGGCGTC TGCCCTCAGC TGCCATCGCT GAGAAGACGG CACCTGTTAA CAAAATGAGC 
GGTTACAGCC AAGATCCAGA AAGAGAGGAG GAAGAGGTTT CCAAGGGCTT CCTGGCCCCC 
AAACCAATAA GCCATTCTGT ATCTTCGGTT AACCTACGGT TTGGAGGAAG GACAACGATG 
AAGACTGTGG TGTGCAAGAT GAACCCCATG CCAGACACAG CTTCCTGTGG CTCTGAAGTT 
AAAAAGTGGT GGACCAGACA GCTGACCGTG GAGAGTGACG AGAGTGGAGA TGACCTCCTG 
GATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nalcn ( XM_017316064.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171553.1 Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X4, mRNA.

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CloneID	OMu74949
Clone ID Related Accession (Same CDS sequence)	XM_017316064.1 , XM_017316064.2
Accession Version	XM_017316064.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sodium leak channel non-selective protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATTGCAT TGCTGGTTCT GGCCCAGTCT 
GTGTTGCTAT CTGTCAAGTG GGATGTTGAC GATCCTGTGA CGGTTCCTTT GGCAACAATG 
TCAGTTGTCT TCACCTTCAT CTTTGTCTTA GAGGTTACAA TGAAGATTAT AGCAATGTCA 
CCAGCTGGAT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCTTGGTGAC ATCTCTTGGT 
GTTGTGTGGG TGGTGCTCCA TTTTGCTCTG CTGAATGCAT ACACCTACAT GATGGGAGCC 
TGCGTGATTG TCTTTAGATT TTTCTCCATC TGTGGGAAGC ATGTGACATT AAAGATGCTC 
CTTCTGACTG TGGTTGTCAG CATGTACAAG AGCTTTTTTA TCATCGTAGG AATGTTTCTC 
TTGCTGCTGT GCTATGCCTT TGCTGGAGTT GTTCTGTTTG GTACCGTAAA GTATGGTGAG 
AACATTAACA GGCATGCCAA TTTTTCCTCA GCTGGCAAAG CCATTACTGT ATTGTTTCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGATT GTATGGTTCA ACCTCCATTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GCAATTATGC AGGGGCACTT 
ATGTACTTCT GCTCATTCTA TGTCATCATT GCCTACATCA TGCTGAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC TTTGTTTTAT TCCACTGAAG AGGACCAGCT TTTGAGTTAC 
AATGATCTTC GCCATTTTCA AATCATATGG AACATGGTAG ATGATAAAAG AGAGGGTGTG 
ATCCCCACTT TCCGAGTGAA GTTCCTGCTA CGGCTGCTGC GTGGGAGGCT GGAAGTGGAT 
CTTGATAAAG ACAAGCTCCT GTTTAAGCAT ATGTGCTATG AGATGGAGAG GCTGCACAAT 
GGTGGTGATG TCACCTTCCA TGATGTCTTA AGCATGCTCT CCTATCGCTC TGTAGACATC 
AGAAAAAGCC TGCAGCTAGA GGAGCTGCTG GCAAGGGAGC AGCTAGAATA CACCATAGAG 
GAGGAGGTGG CTAAGCAGAC TATTCGCATG TGGCTGAAGA AGTGCTTGAA ACGCATCCGG 
GCTAAACAAC AGCAGTCGTG CAGCATCATC CACAGCCTGA GAGAGAGCCA GGAGCAAGAG 
CGGAGCCGGC TATTCCTGAA TCCTCCCAGC ATTGAGACCA CCCAGCCAAG TGAGGACAGC 
AACGCCAACA GCCAGGACCA CAGTATGCAA CCTGAGACAA GCAGTCAGCA GCAGCTCTTA 
AGCCCTACTC TGTCAGACAG AGGAGGAAGC CGACAGGATG CAGCAGATAC TGGAAAACCC 
CAAAGGAAGA TTGGGCAATG GCGTCTGCCC TCAGCCCCCA AACCAATAAG CCATTCTGTA 
TCTTCGGTTA ACCTACGGTT TGGAGGAAGG ACAACGATGA AGACTGTGGT GTGCAAGATG 
AACCCCATGC CAGACACAGC TTCCTGTGGC TCTGAAGTTA AAAAGTGGTG GACCAGACAG 
CTGACCGTGG AGAGTGACGA GAGTGGAGAT GACCTCCTGG ATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171553.1
CDS	87..5354
Translation	MGPTVVLYLPPKANFSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFLNPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAPKPISHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_017316064.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium leak channel, non-selective (Nalcn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316064.1

ORF Insert Sequence:

ATGGGCCCTA CTGTGGTTTT GTACCTGCCT CCCAAAGCTA ACTTCAGCAT GCTCAAAAGA 
AAGCAGAGTT CCAGGGTGGA AGCCCAGCCA GTTACTGACT TTGGTCCTGA TGAATCTCTG 
TCAGACAATG CTGACATACT CTGGATTAAT AAGCCATGGG TGCACTCTCT GCTGCGCATC 
TGTGCCATCA TCAGCGTCAT CTCAGTGTGC ATGAACACAC CTATGACCTT TGAGCACTAT 
CCTCCTCTTC AGTATGTGAC CTTCACCTTG GACACTTTAT TGATGTTCCT CTACACTGCA 
GAAATGATAG CAAAGATGCA CATACGGGGA ATTGTCAAGG GTGATAGCTC CTATGTGAAG 
GATCGCTGGT GTGTTTTTGA TGGATTCATG GTCTTTTGCC TTTGGGTATC TCTTGTGTTA 
CAGGTGTTTG AAATAGCTGA CATAGTTGAT CAGATGTCAC CTTGGGGCAT GCTGCGGATC 
CCACGGCCAC TCATTATGAT CCGGGCTTTC AGGATTTATT TCCGATTCGA ACTGCCAAGG 
ACAAGAATTA CAAACATTTT AAAGCGGTCA GGAGAACAAA TATGGAGTGT TTCCATTTTC 
CTCCTTTTCT TCCTTCTTCT GTATGGGATT TTAGGAGTTC AAATGTTTGG AACATTTACC 
TACCACTGTG TAGTCAATGA CACAAAGCCA GGAAATGTAA CCTGGAATAG CTTAGCTATT 
CCAGATACGC ACTGCTCCCC AGAGCTAGAA GAAGGCTATC AATGCCCGCC AGGATTTAAA 
TGCATGGACC TGGAAGACCT GGGACTTAGC AGGCAAGAGC TGGGCTACAG TGGCTTTAAT 
GAGATAGGCA CGAGTATATT CACAGTCTAT GAGGCTTCAT CTCAGGAAGG CTGGGTATTC 
CTCATGTACA GAGCAATCGA CAGCTTTCCC CGCTGGCGCT CCTACTTCTA CTTCATCACA 
CTGATTTTCT TCCTTGCTTG GCTTGTCAAG AATGTGTTTA TTGCTGTCAT CATTGAGACA 
TTTGCAGAAA TCAGAGTACA ATTTCAACAA ATGTGGGGAA CTCGGAGCAG CACAACTTCT 
ACAGCCACCA CACAGATGTT CCATGAAGAT GCCGCTGGTG GTTGGCAGCT GGTAGCTGTG 
GATGTCAACA AGCCCCAGGG ACGTGCCCCA GCCTGCCTAC AGAAAATGAT GCGGTCATCG 
GTTTTCCACA TGTTTATCCT GAGCATGGTG ACTGTGGATG TAATAGTTGC TGCCAGCAAT 
TACTACAAGG GAGAGAACTT CAGAAGGCAG TATGATGAAT TCTACCTTGC AGAGGTGGCT 
TTTACAGTCC TTTTCGACCT GGAAGCACTC CTGAAGATAT GGTGTTTGGG GTTTACTGGC 
TACATCAGCT CATCTCTCCA CAAGTTTGAA CTATTGCTTG TTATTGGGAC GACGCTTCAT 
GTGTACCCAG ATCTTTATCA TTCTCAGTTC ACATACTTCC AGGTTCTGCG GGTAGTCCGG 
CTTATTAAGA TTTCCCCAGC ATTGGAAGAC TTTGTGTACA AGATATTTGG TCCTGGGAAA 
AAACTTGGAA GCTTGGTGGT GTTCACTGCC AGCCTCCTGA TAGTTATGTC AGCCATCAGT 
TTGCAGATGT TCTGTTTTGT CGAAGAACTG GACAGATTCA CCACTTTTCC AAGGGCATTT 
ATGTCTATGT TCCAGATCCT CACCCAGGAA GGATGGGTGG ATGTGATGGA TCAGACTCTG 
AATGCTGTGG GGCATATGTG GGCACCACTG GTTGCCATCT ATTTCATCCT CTATCATCTC 
TTTGCAACTT TGATCCTCCT GAGTTTGTTT GTTGCTGTTA TTTTGGACAA CTTAGAACTT 
GATGAAGATC TAAAGAAGCT CAAACAACTA AAGCAAAGTG AAGCGAACGC GGACACCAAA 
GAAAAACTCC CTTTGCGCTT GAGAATCTTC GAAAAATTTC CAAACAGACC GCAAATGGTG 
AAAATCTCAA AACTTCCTTC AGATTTTACA GTTCCTAAAA TCAGGGAAAG CTTCATGAAG 
CAGTTCATTG ACCGCCAGCA ACAGGACACC TGCTGTCTCT TCAGAATCCT CCCCTCTACC 
TCTTCCTCAT CATGTGACAA CCCCAAGAAG CCCACAGCTG AAGACAACAA ATACATTGAT 
CAAAAACTCC GCAAGTCTGT TTTCAGCATA AGGGCGAGGA ACCTACTGGA AAAGGAGACT 
GCAGTCACAA AAATCTTAAG AGCTTGCACT CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCAG CAAAGGAGAG GTCGATCCTC AGCGTGCAGC ATCACATCCG CCAGGAGCGC 
AGGTCACTAA GACATGGATC CAACAGCCAG AGGATCAGCA GGGGGAAATC TCTTGAAACT 
TTAACTCAAG ACCATTCAAA TACAGTGCGC TACAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAAATGA TTCAGGAGAA GAAGGAACAA GCCGAGATGA AAAGGAAGGT GCAAGAAGAG 
GAACTGCGGG AGAACCACCC ATACTTTGAC AAGCCTCTCT TCATCGTGGG GCGGGAGCAC 
AGGTTCAGAA ACTTCTGCCG CGTGGTGGTT CGAGCACGCT TCAATGCATC CAAAACAGAC 
CCTGTCACGG GAGCTGTGAA AAATACAAAG TACCACCAAC TTTATGATTT GCTGGGACTT 
GTCACCTACC TGGACTGGGT CATGATCACT GTAACCATCT GCTCTTGCAT TTCTATGATG 
TTCGAATCCC CCTTCCGAAG AGTCATGCAT GCACCCACTT TACAGATCGC AGAATATGTG 
TTTGTAATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTGTTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTTGGT GGCGTCATGG ACATATTTAT CTATCTTGTG 
AGTTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CCGAGTCAGG AGCCCAGCTA 
CTGATGGTTC TTCGGTGCCT AAGACCTCTT CGGATATTCA AACTTGTGCC CCAAATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGTTTC AAGGAAATAT TTTTGGTCTC CATTCTGTTG 
TTGACGTTAA TGCTTGTTTT TGCAAGCTTT GGGGTTCAGC TCTTTGCTGG AAAGTTAGCC 
AAGTGCAACG ACCCCAACAT TATCAGAAGG GAAGACTGTA ATGGCATCTT CAGAATTAAT 
GTAAGTGTGT CCAAGAACTT AAATTTAAAA CTAAGACCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC AAATCCTCGG AACTTTAACT TCGACAATGT GGGAAATGCC 
ATGCTGGCAT TATTTGAAGT TCTGTCCTTG AAAGGCTGGG TAGAAGTGAG GGATGTCATT 
ATTCATCGTG TGGGGCCGAT CCACGGAATC TATATTCATG TTTTCGTATT CCTGGGTTGC 
ATGATCGGAC TGACGCTTTT TGTCGGTGTA GTTATTGCTA ACTTCAATGA AAACAAGGGG 
ACAGCCCTGC TGACGGTAGA TCAGAGACGA TGGGAAGATC TCAAGAGCAG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC TCCTCGGCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATTGCAT TGCTGGTTCT GGCCCAGTCT 
GTGTTGCTAT CTGTCAAGTG GGATGTTGAC GATCCTGTGA CGGTTCCTTT GGCAACAATG 
TCAGTTGTCT TCACCTTCAT CTTTGTCTTA GAGGTTACAA TGAAGATTAT AGCAATGTCA 
CCAGCTGGAT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCTTGGTGAC ATCTCTTGGT 
GTTGTGTGGG TGGTGCTCCA TTTTGCTCTG CTGAATGCAT ACACCTACAT GATGGGAGCC 
TGCGTGATTG TCTTTAGATT TTTCTCCATC TGTGGGAAGC ATGTGACATT AAAGATGCTC 
CTTCTGACTG TGGTTGTCAG CATGTACAAG AGCTTTTTTA TCATCGTAGG AATGTTTCTC 
TTGCTGCTGT GCTATGCCTT TGCTGGAGTT GTTCTGTTTG GTACCGTAAA GTATGGTGAG 
AACATTAACA GGCATGCCAA TTTTTCCTCA GCTGGCAAAG CCATTACTGT ATTGTTTCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGATT GTATGGTTCA ACCTCCATTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GCAATTATGC AGGGGCACTT 
ATGTACTTCT GCTCATTCTA TGTCATCATT GCCTACATCA TGCTGAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC TTTGTTTTAT TCCACTGAAG AGGACCAGCT TTTGAGTTAC 
AATGATCTTC GCCATTTTCA AATCATATGG AACATGGTAG ATGATAAAAG AGAGGGTGTG 
ATCCCCACTT TCCGAGTGAA GTTCCTGCTA CGGCTGCTGC GTGGGAGGCT GGAAGTGGAT 
CTTGATAAAG ACAAGCTCCT GTTTAAGCAT ATGTGCTATG AGATGGAGAG GCTGCACAAT 
GGTGGTGATG TCACCTTCCA TGATGTCTTA AGCATGCTCT CCTATCGCTC TGTAGACATC 
AGAAAAAGCC TGCAGCTAGA GGAGCTGCTG GCAAGGGAGC AGCTAGAATA CACCATAGAG 
GAGGAGGTGG CTAAGCAGAC TATTCGCATG TGGCTGAAGA AGTGCTTGAA ACGCATCCGG 
GCTAAACAAC AGCAGTCGTG CAGCATCATC CACAGCCTGA GAGAGAGCCA GGAGCAAGAG 
CGGAGCCGGC TATTCCTGAA TCCTCCCAGC ATTGAGACCA CCCAGCCAAG TGAGGACAGC 
AACGCCAACA GCCAGGACCA CAGTATGCAA CCTGAGACAA GCAGTCAGCA GCAGCTCTTA 
AGCCCTACTC TGTCAGACAG AGGAGGAAGC CGACAGGATG CAGCAGATAC TGGAAAACCC 
CAAAGGAAGA TTGGGCAATG GCGTCTGCCC TCAGCCCCCA AACCAATAAG CCATTCTGTA 
TCTTCGGTTA ACCTACGGTT TGGAGGAAGG ACAACGATGA AGACTGTGGT GTGCAAGATG 
AACCCCATGC CAGACACAGC TTCCTGTGGC TCTGAAGTTA AAAAGTGGTG GACCAGACAG 
CTGACCGTGG AGAGTGACGA GAGTGGAGAT GACCTCCTGG ATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Nalcn
Entrez Gene ID	338370
Full Name	sodium leak channel, non-selective
Synonyms	A530023G15Rik,AI849508,Vgcnl1
General protein information	Preferred Names sodium leak channel, non-selective Names sodium leak channel non-selective protein voltage gated channel like 1 voltage gated channel-like protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 E5

Pan troglodytes (chimpanzee)	NALCN	XP_001149573.1
Canis lupus familiaris (dog)	NALCN	XP_542654.4
Bos taurus (cattle)	NALCN	XP_002692026.1
Rattus norvegicus (Norway rat)	Nalcn	NP_705894.1
Drosophila melanogaster (fruit fly)	na	NP_001096981.1
Homo sapiens (human)	NALCN	NP_443099.1
Caenorhabditis elegans (roundworm)	nca-2	NP_498054.2
Mus musculus (house mouse)	Nalcn	NP_796367.3
Caenorhabditis elegans (roundworm)	unc-77	NP_741413.2
Gallus gallus (chicken)	NALCN	XP_416967.2
Danio rerio (zebrafish)	nalcn	NP_001017549.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003921	XP_318373.5
Xenopus tropicalis (tropical clawed frog)	nalcn	XP_002937125.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Nalcn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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Target ORF information:

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