nalcn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nalcn
Entrez Gene ID	100492286
Full Name	sodium leak channel, non selective
General protein information	Preferred Names sodium leak channel, non selective Names sodium leak channel non-selective protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012957712.2	XP_012813166.1	sodium leak channel non-selective protein isoform X2
XM_002937079.4	XP_002937125.2	sodium leak channel non-selective protein isoform X1
XM_012957711.2	XP_012813165.1	sodium leak channel non-selective protein isoform X1
XM_012957711.3	XP_012813165.1	sodium leak channel non-selective protein isoform X1
XM_012957710.2	XP_012813164.1	sodium leak channel non-selective protein isoform X1
XM_002937079.5	XP_002937125.2	sodium leak channel non-selective protein isoform X1
XM_012957710.3	XP_012813164.1	sodium leak channel non-selective protein isoform X1
XM_012957712.3	XP_012813166.1	sodium leak channel non-selective protein isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NALCN	XP_001149573.1
Canis lupus familiaris (dog)	NALCN	XP_542654.4
Bos taurus (cattle)	NALCN	XP_002692026.1
Rattus norvegicus (Norway rat)	Nalcn	NP_705894.1
Drosophila melanogaster (fruit fly)	na	NP_001096981.1
Homo sapiens (human)	NALCN	NP_443099.1
Caenorhabditis elegans (roundworm)	nca-2	NP_498054.2
Mus musculus (house mouse)	Nalcn	NP_796367.3
Caenorhabditis elegans (roundworm)	unc-77	NP_741413.2
Gallus gallus (chicken)	NALCN	XP_416967.2
Danio rerio (zebrafish)	nalcn	NP_001017549.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003921	XP_318373.5
Xenopus tropicalis (tropical clawed frog)	nalcn	XP_002937125.2

Gene Ontology

The following nalcn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nalcn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa52534	XM_012957711.3 Latest version!	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa52534	XM_012957710.3 Latest version!	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa52534	XM_002937079.5 Latest version!	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa52535	XM_012957712.3 Latest version!	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa05901	XM_012957710.2 Latest version!	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa05901	XM_002937079.4 Latest version!	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa05901	XM_012957711.2 Latest version!	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa35339	XM_012957712.2 Latest version!	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nalcn ( XM_012957711.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813165.1 Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa52534
Clone ID Related Accession (Same CDS sequence)	XM_012957711.3 , XM_002937079.5 , XM_012957710.3
Accession Version	XM_012957711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813165.1
CDS	349..5583
Translation	MKTKSDNSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDTSYVKDRWCVFDGFMVFCLWVSLVLQVFEIAKIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTFHCVTNDTVHGNLTWNNLAIPDTHCSPEGEGYQCPRGFRCVDLKELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSIFHMFILSMVAVDVIVAASNYYKGENFKRQYDEFYLAEVAFTMLFDLEALLKIWCLGFTGYISSSLHKFELLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTSCLLRRLPSASSSSCDHSKRSGVEENKYIDQKLRKSVFSIRARNLLEKETAVNKILRDCTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVIVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPNYVPAKSGTQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHILTREDCHGIFRINVSVSKNLNLKLKPEEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRARMYDITQHPFFKRTIAVLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKITAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFALLNEYTYMMGACIIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDNSKYWETDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSPNDLRHYQIIWNMVDDKREGVIPAFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHSGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNLSNQDNAQPETSSQQQLLSPTLSDRGGYRQDSIEAGKPQRKFGQWRLPAAPKPISHSVSSVNLRFGGRSTMKSVVCKMNAMSDAASCGSEVKKWWTRQLTVESDESGDDLLDM

Target ORF information:

RefSeq Version	XM_012957711.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957711.3

ORF Insert Sequence:

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_002937079.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937125.2 Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA.

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CloneID	OXa52534
Clone ID Related Accession (Same CDS sequence)	XM_012957711.3 , XM_002937079.5 , XM_012957710.3
Accession Version	XM_002937079.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937079.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937125.2
CDS	549..5783
Translation	MKTKSDNSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDTSYVKDRWCVFDGFMVFCLWVSLVLQVFEIAKIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTFHCVTNDTVHGNLTWNNLAIPDTHCSPEGEGYQCPRGFRCVDLKELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSIFHMFILSMVAVDVIVAASNYYKGENFKRQYDEFYLAEVAFTMLFDLEALLKIWCLGFTGYISSSLHKFELLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTSCLLRRLPSASSSSCDHSKRSGVEENKYIDQKLRKSVFSIRARNLLEKETAVNKILRDCTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVIVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPNYVPAKSGTQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHILTREDCHGIFRINVSVSKNLNLKLKPEEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRARMYDITQHPFFKRTIAVLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKITAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFALLNEYTYMMGACIIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDNSKYWETDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSPNDLRHYQIIWNMVDDKREGVIPAFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHSGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNLSNQDNAQPETSSQQQLLSPTLSDRGGYRQDSIEAGKPQRKFGQWRLPAAPKPISHSVSSVNLRFGGRSTMKSVVCKMNAMSDAASCGSEVKKWWTRQLTVESDESGDDLLDM

Target ORF information:

RefSeq Version	XM_002937079.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937079.5

ORF Insert Sequence:

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_012957710.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813164.1 Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA.

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CloneID	OXa52534
Clone ID Related Accession (Same CDS sequence)	XM_012957711.3 , XM_002937079.5 , XM_012957710.3
Accession Version	XM_012957710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813164.1
CDS	384..5618
Translation	MKTKSDNSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDTSYVKDRWCVFDGFMVFCLWVSLVLQVFEIAKIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTFHCVTNDTVHGNLTWNNLAIPDTHCSPEGEGYQCPRGFRCVDLKELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSIFHMFILSMVAVDVIVAASNYYKGENFKRQYDEFYLAEVAFTMLFDLEALLKIWCLGFTGYISSSLHKFELLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTSCLLRRLPSASSSSCDHSKRSGVEENKYIDQKLRKSVFSIRARNLLEKETAVNKILRDCTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVIVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPNYVPAKSGTQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHILTREDCHGIFRINVSVSKNLNLKLKPEEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRARMYDITQHPFFKRTIAVLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKITAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFALLNEYTYMMGACIIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDNSKYWETDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSPNDLRHYQIIWNMVDDKREGVIPAFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHSGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNLSNQDNAQPETSSQQQLLSPTLSDRGGYRQDSIEAGKPQRKFGQWRLPAAPKPISHSVSSVNLRFGGRSTMKSVVCKMNAMSDAASCGSEVKKWWTRQLTVESDESGDDLLDM

Target ORF information:

RefSeq Version	XM_012957710.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957710.3

ORF Insert Sequence:

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_012957712.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813166.1 Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA.

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CloneID	OXa52535
Clone ID Related Accession (Same CDS sequence)	XM_012957712.3
Accession Version	XM_012957712.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAAC GAAAGCAAAG CTCAAGAGTG GAAGCACAGC CAGTCACAGA CTTTGGCCCC 
GATGAATCTC TGTCAGACAA TGCTGATATC CTGTGGATTA ACAAACCATG GGTTCACTCT 
TTACTGCGCA TCTGCGCAAT CATTAGTGTT ATTTCTGTTT GTATGAACAC CCCAAAGACA 
TTTGAACATT ACCCACCCCT CCAGTATGTC ACGTTTGCAC TCGATACCCT ACTTATGTTT 
CTTTACACCG CAGAAATGAT TGCAAAAATG CACATAAGAG GCATTGTAAA GGGTGATACT 
TCCTATGTAA AGGATCGCTG GTGCGTCTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCATTGGTGT TGCAGGTATT TGAAATCGCC AAAATCGTTG ACCAAATGTC CCCATGGGGC 
ATGCTGCGGA TCCCACGACC TCTGATAATG ATTCGTGCCT TCCGTATATA CTTCCGCTTT 
GAACTCCCAA GAACGAGGAT AACAAATATC TTGAAGCGTT CAGGAGAGCA AATTTGGAGT 
GTTTCCATAT TTTTATTATT CTTTCTTCTA TTATACGGGA TTCTCGGAGT TCAGATGTTT 
GGAACATTTA CCTTCCATTG TGTTACAAAT GACACAGTAC ATGGAAATCT GACTTGGAAT 
AATTTAGCCA TTCCAGATAC TCACTGCTCC CCTGAAGGTG AAGGATACCA GTGTCCTCGT 
GGTTTTAGGT GCGTGGATCT GAAAGAACTT GGACTGAGCA GGCAAGAGCT TGGCTACAGT 
GGCTTTAATG AAATAGGAAC CAGCATATTT ACGGTTTATG AAGCTGCATC TCAAGAAGGA 
TGGGTTTTTC TTATGTATAG AGCAATAGAC AGCTTCCCCA GATGGCGATC GTACTTCTAT 
TTTATTACCT TAATTTTCTT CCTTGCCTGG CTTGTCAAGA ATGTCTTCAT TGCTGTAATT 
ATTGAAACAT TTGCTGAAAT TCGAGTGCAG TTTCAACAGA TGTGGGGTTC TCGAAGTAGT 
ACTACCTCAA CAGCTACCAC ACAGATGTTT CACGAGGATG CAGCAGGGGG CTGGCAGCTA 
GTGGCAGTTG ATGTGAACAA GCCCCAAGGC AGAGCACCAG CTTGTCTACA GAAAATGATG 
AGATCTTCCA TTTTCCACAT GTTTATTCTG AGCATGGTTG CTGTGGATGT AATTGTTGCT 
GCCAGCAATT ACTACAAAGG AGAGAATTTT AAAAGACAAT ATGATGAGTT TTATCTGGCC 
GAGGTGGCAT TCACCATGCT GTTTGATTTG GAAGCTCTTT TAAAGATTTG GTGCCTGGGC 
TTTACAGGAT ATATCAGTTC ATCACTACAC AAATTTGAGC TTTTGCTTGT AGTTGGAACT 
ACTCTTCATA TTTATCCTGA TCTTTACCAT TCACAGTTTA CATACTTTCA GGTTCTAAGG 
GTGGTGCGAC TGATTAAGAT TTCTCCTGCC TTAGAAGACT TTGTATATAA AATATTTGGA 
CCTGGCAAGA AATTAGGAAG TCTTGTGGTT TTCACCGCTA GCCTATTAAT TGTAATGTCT 
GCCATCAGTT TACAGATGTT CTGCTTTGTG GAAGAGCTGG ACCGTTTTAC AACATTTCCA 
AGGGCATTTA TGTCAATGTT TCAGATTCTG ACACAGGAAG GATGGGTGGA TGTCATGGAT 
CAAACACTTA ATGCCGTTGG GCATATGTGG GCACCAGTTG TTGCAATTTA CTTCATACTT 
TATCATCTTT TTGCTACTTT GATATTGCTT AGTTTGTTTG TGGCTGTTAT CCTGGACAAT 
CTGGAGCTTG ATGAAGACTT GAAAAAGCTT AAACAGTTGA AACAAAGTGA AGCAAATGCC 
GACACCAAAG AAAAATTACC ACTACGCCTA CGTATCTTTG AGAAATTCCC CAACAGACCG 
CAAATGGTGA AGATTTCCAA GCTTCCTTCT GATTTCACAG TTCCAAAAAT AAGGGAGAGT 
TTCATGAAGC AGTTTATTGA CCGCCAGCAA CAAGACACAA GCTGTTTATT AAGAAGGCTT 
CCATCTGCTT CTTCTTCTTC ATGTGATCAT TCCAAACGAT CAGGAGTAGA AGAGAATAAG 
TACATTGACC AAAAGCTTCG TAAATCAGTG TTTAGTATAA GAGCAAGGAA TCTTCTGGAA 
AAGGAAACTG CAGTTAATAA AATCCTCAGA GATTGCACAA GACAGCGCAT GCTGAGTGGA 
TCCTTTGAAG GACAGCCTGC AAAGGAGAGA TCAATACTGA GTGTGCAGCA CCACATAAGA 
CAAGAACGGA GATCACTAAG ACATGGATCA AACAGTCAGA GAATCAGCAG GGGGAAGTCT 
CTGGAAACAC TGACACAAGA CCACTCCAAT ACAGTCAGAT ACAGAAATGC ACAGAGGGAA 
GACAGTGAGA TCAAGATGAT CCAGGAAAAA AAAGAACAAG CAGAAATGAA AAGGAAAGTG 
CAGGAAGAAG AGTTAAGAGA AAACCACCCA TACTTTGATA AGCCACTGTT TATAGTTGGC 
CGTGAACACC GGTTCCGAAA CTTCTGCAGG GTAATAGTTC GGGCACGATT TAATGCCTCA 
AAGACAGATC CTGTTACAGG AGCAGTGAAA AACACCAAAT ATCATCAACT TTATGATTTG 
TTGGGACTAG TGACCTATCT AGACTGGGTT ATGATTGTTG TTACCATATG TTCCTGCATA 
TCAATGATGT TTGAATCTCC TTTTAGAAGA GTAATGCACA CCCCCACGTT ACAGATTGCA 
GAATATGTTT TTGTCATATT CATGAGCATT GAACTGAACT TGAAGATTAT GGCAGATGGC 
TTATTTTTTA CTCCAACGGC TGTTATACGA GACTTTGGGG GTGTTATGGA TATCTTCATT 
TATCTTGTTA GTTTAATCTT CTTATGCTGG ATGCCTAATT ATGTTCCCGC AAAATCTGGA 
ACCCAACTAT TAATGGTGCT GCGCTGCTTG AGACCACTGA GAATATTCAA GCTTGTACCA 
CAGATGAGGA AAGTCGTACG GGAACTTTTC AGTGGGTTTA AAGAAATATT TCTGGTTTCT 
ATTCTTCTGC TGACACTGAT GCTTGTGTTT GCAAGCTTTG GCGTGCAGCT CTTTGCAGGG 
AAACTGGCAA AGTGCAATGA CCCACACATA CTCACCAGGG AAGACTGTCA TGGAATATTT 
AGAATTAATG TCAGTGTTTC GAAAAATTTA AATCTGAAAT TAAAACCAGA GGAAAAGAAG 
CCTGGATTCT GGGTTCCAAG AGTCTGGGCA AATCCCCGTA ATTTTAATTT TGACAATGTA 
GGAAATGCCA TGCTGGCCTT GTTTGAGGTA TTGTCTTTGA AGGGCTGGGT TGAAGTGCGA 
GATGTTATTA TTCATCGAGT TGGACCGATC CATGGAATTT ATATTCATGT CTTTGTGTTT 
CTTGGCTGTA TGATTGGCTT GACTCTCTTT GTTGGTGTGG TTATTGCTAA CTTTAATGAG 
AATAAGGGTA CAGCTTTACT GACTGTTGAT CAAAGGAGAT GGGAAGATCT GAAAAGCAGA 
CTTAAGATAG CCCAACCACT TCATCTCCCC CCACGTCCAG ATAATGATGG TTTCAGAGCC 
CGCATGTATG ACATAACGCA ACACCCTTTT TTCAAGAGGA CTATTGCTGT ACTTGTTTTA 
GCACAGTCTG TGCTTCTGTC AGTAAAGTGG GATGTTGAAG ACCCTGTGAC GGTTCCACTG 
GCAACTATGT CTGTGGTTTT CACCTTCATC TTTGTACTTG AGGTAACCAT GAAGATAACA 
GCTATGTCAC CAGCTGGATT TTGGCAAAGT AGAAGAAATC GCTATGATCT CCTTGTCACA 
TCTCTTGGTG TGATCTGGGT GATACTTCAT TTTGCTTTAC TGAATGAATA CACTTATATG 
ATGGGTGCTT GTATTATCGT CTTTCGTTTT TTCTCTATCT GTGGCAAACA TGTCACACTG 
AAAATGTTAT TATTGACTGT GGTAGTCAGC ATGTACAAGA GCTTCTTCAT AATAGTAGGA 
ATGTTTCTGC TGCTTCTCTG CTATGCTTTT GCCGGTGTTG TTCTGTTTGG AACAGTGAAA 
TATGGAGAAA ACATAAACAG GCATGCTAAC TTTTCCACAG CAGGGAAAGC TATAACTGTG 
CTATTCAGAA TAGTAACTGG AGAAGACTGG AACAAAATAA TGCATGACTG CATGGTACAG 
CCTCCTTTTT GCACTCCAGA TAACAGCAAA TATTGGGAAA CAGATTGTGG AAATTATGCA 
GGGGCACTTA TGTACTTCTG TTCATTTTAT GTCATCATTG CTTACATCAT GTTAAATCTA 
CTGGTTGCCA TAATTGTGGA AAACTTTTCC CTCTTCTACT CTACTGAAGA AGACCAGCTC 
CTAAGCCCTA ATGATCTGCG CCATTACCAA ATAATATGGA ATATGGTTGA TGATAAAAGA 
GAGGGGGTTA TTCCAGCTTT TCGTGTGAAG TTCCTTCTGA GACTGCTACG CGGGAGGCTG 
GAGGTTGACT TAGATAAGGA CAAGCTTTTG TTCAAACACA TGTGCTATGA AATGGAGAGA 
CTGCACAGCG GGGGAGATGT CACTTTCCAT GATGTACTTA GCATGCTTTC CTACAGATCA 
GTGGATATCA GAAAGAGCCT TCAGCTGGAG GAGTTGCTAG CCAGAGAACA GCTAGAATAC 
ACAATAGAAG AAGAAGTAGC TAAACAAACA ATACGAATGT GGTTGAAAAA GTGTTTGAAA 
CGCATTCGAG CAAAACAACA GCAATCATGC AGTATTATAC ACAGTTTGAG AGAGAGCCAA 
CAGCAGGAAC TGAGCAGGTT TCTGAACCCT CCTAGCATTG AGACCACACA GCCAAGTGAA 
GATATGAACC TAAGCAACCA AGACAATGCA CAGCCAGAGA CAAGTAGCCA ACAACAGCTT 
CTTAGCCCAA CGCTTTCTGA CAGAGGAGGT TATCGTCAGG ATTCAATTGA AGCTGGTAAA 
CCTCAGAGAA AGTTTGGGCA GTGGCGATTA CCAGCAGCAC CAAAACCAAT AAGCCATTCT 
GTGTCCTCTG TGAACCTACG ATTTGGAGGA CGGTCAACAA TGAAGTCTGT AGTGTGCAAG 
ATGAACGCCA TGTCTGATGC TGCCTCCTGT GGTTCTGAAG TCAAGAAATG GTGGACAAGA 
CAACTGACAG TAGAAAGTGA TGAGAGTGGT GATGACCTTC TTGATATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813166.1
CDS	443..5653
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDTSYVKDRWCVFDGFMVFCLWVSLVLQVFEIAKIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTFHCVTNDTVHGNLTWNNLAIPDTHCSPEGEGYQCPRGFRCVDLKELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSIFHMFILSMVAVDVIVAASNYYKGENFKRQYDEFYLAEVAFTMLFDLEALLKIWCLGFTGYISSSLHKFELLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTSCLLRRLPSASSSSCDHSKRSGVEENKYIDQKLRKSVFSIRARNLLEKETAVNKILRDCTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVIVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPNYVPAKSGTQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHILTREDCHGIFRINVSVSKNLNLKLKPEEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRARMYDITQHPFFKRTIAVLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKITAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFALLNEYTYMMGACIIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDNSKYWETDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSPNDLRHYQIIWNMVDDKREGVIPAFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHSGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNLSNQDNAQPETSSQQQLLSPTLSDRGGYRQDSIEAGKPQRKFGQWRLPAAPKPISHSVSSVNLRFGGRSTMKSVVCKMNAMSDAASCGSEVKKWWTRQLTVESDESGDDLLDM

Target ORF information:

RefSeq Version	XM_012957712.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957712.3

ORF Insert Sequence:

ATGTTGAAAC GAAAGCAAAG CTCAAGAGTG GAAGCACAGC CAGTCACAGA CTTTGGCCCC 
GATGAATCTC TGTCAGACAA TGCTGATATC CTGTGGATTA ACAAACCATG GGTTCACTCT 
TTACTGCGCA TCTGCGCAAT CATTAGTGTT ATTTCTGTTT GTATGAACAC CCCAAAGACA 
TTTGAACATT ACCCACCCCT CCAGTATGTC ACGTTTGCAC TCGATACCCT ACTTATGTTT 
CTTTACACCG CAGAAATGAT TGCAAAAATG CACATAAGAG GCATTGTAAA GGGTGATACT 
TCCTATGTAA AGGATCGCTG GTGCGTCTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCATTGGTGT TGCAGGTATT TGAAATCGCC AAAATCGTTG ACCAAATGTC CCCATGGGGC 
ATGCTGCGGA TCCCACGACC TCTGATAATG ATTCGTGCCT TCCGTATATA CTTCCGCTTT 
GAACTCCCAA GAACGAGGAT AACAAATATC TTGAAGCGTT CAGGAGAGCA AATTTGGAGT 
GTTTCCATAT TTTTATTATT CTTTCTTCTA TTATACGGGA TTCTCGGAGT TCAGATGTTT 
GGAACATTTA CCTTCCATTG TGTTACAAAT GACACAGTAC ATGGAAATCT GACTTGGAAT 
AATTTAGCCA TTCCAGATAC TCACTGCTCC CCTGAAGGTG AAGGATACCA GTGTCCTCGT 
GGTTTTAGGT GCGTGGATCT GAAAGAACTT GGACTGAGCA GGCAAGAGCT TGGCTACAGT 
GGCTTTAATG AAATAGGAAC CAGCATATTT ACGGTTTATG AAGCTGCATC TCAAGAAGGA 
TGGGTTTTTC TTATGTATAG AGCAATAGAC AGCTTCCCCA GATGGCGATC GTACTTCTAT 
TTTATTACCT TAATTTTCTT CCTTGCCTGG CTTGTCAAGA ATGTCTTCAT TGCTGTAATT 
ATTGAAACAT TTGCTGAAAT TCGAGTGCAG TTTCAACAGA TGTGGGGTTC TCGAAGTAGT 
ACTACCTCAA CAGCTACCAC ACAGATGTTT CACGAGGATG CAGCAGGGGG CTGGCAGCTA 
GTGGCAGTTG ATGTGAACAA GCCCCAAGGC AGAGCACCAG CTTGTCTACA GAAAATGATG 
AGATCTTCCA TTTTCCACAT GTTTATTCTG AGCATGGTTG CTGTGGATGT AATTGTTGCT 
GCCAGCAATT ACTACAAAGG AGAGAATTTT AAAAGACAAT ATGATGAGTT TTATCTGGCC 
GAGGTGGCAT TCACCATGCT GTTTGATTTG GAAGCTCTTT TAAAGATTTG GTGCCTGGGC 
TTTACAGGAT ATATCAGTTC ATCACTACAC AAATTTGAGC TTTTGCTTGT AGTTGGAACT 
ACTCTTCATA TTTATCCTGA TCTTTACCAT TCACAGTTTA CATACTTTCA GGTTCTAAGG 
GTGGTGCGAC TGATTAAGAT TTCTCCTGCC TTAGAAGACT TTGTATATAA AATATTTGGA 
CCTGGCAAGA AATTAGGAAG TCTTGTGGTT TTCACCGCTA GCCTATTAAT TGTAATGTCT 
GCCATCAGTT TACAGATGTT CTGCTTTGTG GAAGAGCTGG ACCGTTTTAC AACATTTCCA 
AGGGCATTTA TGTCAATGTT TCAGATTCTG ACACAGGAAG GATGGGTGGA TGTCATGGAT 
CAAACACTTA ATGCCGTTGG GCATATGTGG GCACCAGTTG TTGCAATTTA CTTCATACTT 
TATCATCTTT TTGCTACTTT GATATTGCTT AGTTTGTTTG TGGCTGTTAT CCTGGACAAT 
CTGGAGCTTG ATGAAGACTT GAAAAAGCTT AAACAGTTGA AACAAAGTGA AGCAAATGCC 
GACACCAAAG AAAAATTACC ACTACGCCTA CGTATCTTTG AGAAATTCCC CAACAGACCG 
CAAATGGTGA AGATTTCCAA GCTTCCTTCT GATTTCACAG TTCCAAAAAT AAGGGAGAGT 
TTCATGAAGC AGTTTATTGA CCGCCAGCAA CAAGACACAA GCTGTTTATT AAGAAGGCTT 
CCATCTGCTT CTTCTTCTTC ATGTGATCAT TCCAAACGAT CAGGAGTAGA AGAGAATAAG 
TACATTGACC AAAAGCTTCG TAAATCAGTG TTTAGTATAA GAGCAAGGAA TCTTCTGGAA 
AAGGAAACTG CAGTTAATAA AATCCTCAGA GATTGCACAA GACAGCGCAT GCTGAGTGGA 
TCCTTTGAAG GACAGCCTGC AAAGGAGAGA TCAATACTGA GTGTGCAGCA CCACATAAGA 
CAAGAACGGA GATCACTAAG ACATGGATCA AACAGTCAGA GAATCAGCAG GGGGAAGTCT 
CTGGAAACAC TGACACAAGA CCACTCCAAT ACAGTCAGAT ACAGAAATGC ACAGAGGGAA 
GACAGTGAGA TCAAGATGAT CCAGGAAAAA AAAGAACAAG CAGAAATGAA AAGGAAAGTG 
CAGGAAGAAG AGTTAAGAGA AAACCACCCA TACTTTGATA AGCCACTGTT TATAGTTGGC 
CGTGAACACC GGTTCCGAAA CTTCTGCAGG GTAATAGTTC GGGCACGATT TAATGCCTCA 
AAGACAGATC CTGTTACAGG AGCAGTGAAA AACACCAAAT ATCATCAACT TTATGATTTG 
TTGGGACTAG TGACCTATCT AGACTGGGTT ATGATTGTTG TTACCATATG TTCCTGCATA 
TCAATGATGT TTGAATCTCC TTTTAGAAGA GTAATGCACA CCCCCACGTT ACAGATTGCA 
GAATATGTTT TTGTCATATT CATGAGCATT GAACTGAACT TGAAGATTAT GGCAGATGGC 
TTATTTTTTA CTCCAACGGC TGTTATACGA GACTTTGGGG GTGTTATGGA TATCTTCATT 
TATCTTGTTA GTTTAATCTT CTTATGCTGG ATGCCTAATT ATGTTCCCGC AAAATCTGGA 
ACCCAACTAT TAATGGTGCT GCGCTGCTTG AGACCACTGA GAATATTCAA GCTTGTACCA 
CAGATGAGGA AAGTCGTACG GGAACTTTTC AGTGGGTTTA AAGAAATATT TCTGGTTTCT 
ATTCTTCTGC TGACACTGAT GCTTGTGTTT GCAAGCTTTG GCGTGCAGCT CTTTGCAGGG 
AAACTGGCAA AGTGCAATGA CCCACACATA CTCACCAGGG AAGACTGTCA TGGAATATTT 
AGAATTAATG TCAGTGTTTC GAAAAATTTA AATCTGAAAT TAAAACCAGA GGAAAAGAAG 
CCTGGATTCT GGGTTCCAAG AGTCTGGGCA AATCCCCGTA ATTTTAATTT TGACAATGTA 
GGAAATGCCA TGCTGGCCTT GTTTGAGGTA TTGTCTTTGA AGGGCTGGGT TGAAGTGCGA 
GATGTTATTA TTCATCGAGT TGGACCGATC CATGGAATTT ATATTCATGT CTTTGTGTTT 
CTTGGCTGTA TGATTGGCTT GACTCTCTTT GTTGGTGTGG TTATTGCTAA CTTTAATGAG 
AATAAGGGTA CAGCTTTACT GACTGTTGAT CAAAGGAGAT GGGAAGATCT GAAAAGCAGA 
CTTAAGATAG CCCAACCACT TCATCTCCCC CCACGTCCAG ATAATGATGG TTTCAGAGCC 
CGCATGTATG ACATAACGCA ACACCCTTTT TTCAAGAGGA CTATTGCTGT ACTTGTTTTA 
GCACAGTCTG TGCTTCTGTC AGTAAAGTGG GATGTTGAAG ACCCTGTGAC GGTTCCACTG 
GCAACTATGT CTGTGGTTTT CACCTTCATC TTTGTACTTG AGGTAACCAT GAAGATAACA 
GCTATGTCAC CAGCTGGATT TTGGCAAAGT AGAAGAAATC GCTATGATCT CCTTGTCACA 
TCTCTTGGTG TGATCTGGGT GATACTTCAT TTTGCTTTAC TGAATGAATA CACTTATATG 
ATGGGTGCTT GTATTATCGT CTTTCGTTTT TTCTCTATCT GTGGCAAACA TGTCACACTG 
AAAATGTTAT TATTGACTGT GGTAGTCAGC ATGTACAAGA GCTTCTTCAT AATAGTAGGA 
ATGTTTCTGC TGCTTCTCTG CTATGCTTTT GCCGGTGTTG TTCTGTTTGG AACAGTGAAA 
TATGGAGAAA ACATAAACAG GCATGCTAAC TTTTCCACAG CAGGGAAAGC TATAACTGTG 
CTATTCAGAA TAGTAACTGG AGAAGACTGG AACAAAATAA TGCATGACTG CATGGTACAG 
CCTCCTTTTT GCACTCCAGA TAACAGCAAA TATTGGGAAA CAGATTGTGG AAATTATGCA 
GGGGCACTTA TGTACTTCTG TTCATTTTAT GTCATCATTG CTTACATCAT GTTAAATCTA 
CTGGTTGCCA TAATTGTGGA AAACTTTTCC CTCTTCTACT CTACTGAAGA AGACCAGCTC 
CTAAGCCCTA ATGATCTGCG CCATTACCAA ATAATATGGA ATATGGTTGA TGATAAAAGA 
GAGGGGGTTA TTCCAGCTTT TCGTGTGAAG TTCCTTCTGA GACTGCTACG CGGGAGGCTG 
GAGGTTGACT TAGATAAGGA CAAGCTTTTG TTCAAACACA TGTGCTATGA AATGGAGAGA 
CTGCACAGCG GGGGAGATGT CACTTTCCAT GATGTACTTA GCATGCTTTC CTACAGATCA 
GTGGATATCA GAAAGAGCCT TCAGCTGGAG GAGTTGCTAG CCAGAGAACA GCTAGAATAC 
ACAATAGAAG AAGAAGTAGC TAAACAAACA ATACGAATGT GGTTGAAAAA GTGTTTGAAA 
CGCATTCGAG CAAAACAACA GCAATCATGC AGTATTATAC ACAGTTTGAG AGAGAGCCAA 
CAGCAGGAAC TGAGCAGGTT TCTGAACCCT CCTAGCATTG AGACCACACA GCCAAGTGAA 
GATATGAACC TAAGCAACCA AGACAATGCA CAGCCAGAGA CAAGTAGCCA ACAACAGCTT 
CTTAGCCCAA CGCTTTCTGA CAGAGGAGGT TATCGTCAGG ATTCAATTGA AGCTGGTAAA 
CCTCAGAGAA AGTTTGGGCA GTGGCGATTA CCAGCAGCAC CAAAACCAAT AAGCCATTCT 
GTGTCCTCTG TGAACCTACG ATTTGGAGGA CGGTCAACAA TGAAGTCTGT AGTGTGCAAG 
ATGAACGCCA TGTCTGATGC TGCCTCCTGT GGTTCTGAAG TCAAGAAATG GTGGACAAGA 
CAACTGACAG TAGAAAGTGA TGAGAGTGGT GATGACCTTC TTGATATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_002937079.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937125.2 Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA.

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CloneID	OXa05901
Clone ID Related Accession (Same CDS sequence)	XM_002937079.4 , XM_012957711.2 , XM_012957710.2
Accession Version	XM_002937079.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937079.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937125.2
CDS	730..5964
Translation	MKTKSDNSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDTSYVKDRWCVFDGFMVFCLWVSLVLQVFEIAKIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTFHCVTNDTVHGNLTWNNLAIPDTHCSPEGEGYQCPRGFRCVDLKELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSIFHMFILSMVAVDVIVAASNYYKGENFKRQYDEFYLAEVAFTMLFDLEALLKIWCLGFTGYISSSLHKFELLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTSCLLRRLPSASSSSCDHSKRSGVEENKYIDQKLRKSVFSIRARNLLEKETAVNKILRDCTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVIVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPNYVPAKSGTQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHILTREDCHGIFRINVSVSKNLNLKLKPEEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRARMYDITQHPFFKRTIAVLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKITAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFALLNEYTYMMGACIIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDNSKYWETDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSPNDLRHYQIIWNMVDDKREGVIPAFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHSGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNLSNQDNAQPETSSQQQLLSPTLSDRGGYRQDSIEAGKPQRKFGQWRLPAAPKPISHSVSSVNLRFGGRSTMKSVVCKMNAMSDAASCGSEVKKWWTRQLTVESDESGDDLLDM

Target ORF information:

RefSeq Version	XM_002937079.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937079.4

ORF Insert Sequence:

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_012957711.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813165.1 Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA.

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CloneID	OXa05901
Clone ID Related Accession (Same CDS sequence)	XM_002937079.4 , XM_012957711.2 , XM_012957710.2
Accession Version	XM_012957711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813165.1
CDS	340..5574
Translation	MKTKSDNSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDTSYVKDRWCVFDGFMVFCLWVSLVLQVFEIAKIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTFHCVTNDTVHGNLTWNNLAIPDTHCSPEGEGYQCPRGFRCVDLKELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSIFHMFILSMVAVDVIVAASNYYKGENFKRQYDEFYLAEVAFTMLFDLEALLKIWCLGFTGYISSSLHKFELLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTSCLLRRLPSASSSSCDHSKRSGVEENKYIDQKLRKSVFSIRARNLLEKETAVNKILRDCTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVIVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPNYVPAKSGTQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHILTREDCHGIFRINVSVSKNLNLKLKPEEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRARMYDITQHPFFKRTIAVLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKITAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFALLNEYTYMMGACIIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDNSKYWETDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSPNDLRHYQIIWNMVDDKREGVIPAFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHSGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNLSNQDNAQPETSSQQQLLSPTLSDRGGYRQDSIEAGKPQRKFGQWRLPAAPKPISHSVSSVNLRFGGRSTMKSVVCKMNAMSDAASCGSEVKKWWTRQLTVESDESGDDLLDM

Target ORF information:

RefSeq Version	XM_012957711.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957711.2

ORF Insert Sequence:

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_012957710.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813164.1 Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA.

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CloneID	OXa05901
Clone ID Related Accession (Same CDS sequence)	XM_002937079.4 , XM_012957711.2 , XM_012957710.2
Accession Version	XM_012957710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813164.1
CDS	899..6133
Translation	MKTKSDNSMLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDTSYVKDRWCVFDGFMVFCLWVSLVLQVFEIAKIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTFHCVTNDTVHGNLTWNNLAIPDTHCSPEGEGYQCPRGFRCVDLKELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSIFHMFILSMVAVDVIVAASNYYKGENFKRQYDEFYLAEVAFTMLFDLEALLKIWCLGFTGYISSSLHKFELLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTSCLLRRLPSASSSSCDHSKRSGVEENKYIDQKLRKSVFSIRARNLLEKETAVNKILRDCTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVIVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPNYVPAKSGTQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHILTREDCHGIFRINVSVSKNLNLKLKPEEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRARMYDITQHPFFKRTIAVLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKITAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFALLNEYTYMMGACIIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDNSKYWETDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSPNDLRHYQIIWNMVDDKREGVIPAFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHSGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNLSNQDNAQPETSSQQQLLSPTLSDRGGYRQDSIEAGKPQRKFGQWRLPAAPKPISHSVSSVNLRFGGRSTMKSVVCKMNAMSDAASCGSEVKKWWTRQLTVESDESGDDLLDM

Target ORF information:

RefSeq Version	XM_012957710.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957710.2

ORF Insert Sequence:

ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG 
ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT 
GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT 
GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA 
AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA 
TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC 
GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT 
GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG 
CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC 
GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA 
GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA 
GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG 
AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT 
TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC 
CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC 
AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA 
CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG 
GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA 
CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG 
GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA 
CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT 
CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT 
GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG 
TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA 
GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC 
GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG 
CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG 
GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA 
GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG 
TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG 
TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC 
TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC 
ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC 
ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA 
CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT 
ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC 
ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA 
CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT 
CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC 
AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA 
CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT 
GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA 
GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC 
AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT 
GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG 
CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG 
AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT 
GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT 
AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA 
CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG 
TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC 
TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC 
AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG 
AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC 
CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT 
TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA 
ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT 
GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG 
AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT 
CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG 
AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT 
GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA 
CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA 
AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT 
TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT 
ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC 
AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT 
GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC 
ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA 
ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG 
GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC 
ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC 
TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA 
TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT 
CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA 
CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA 
CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG 
CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA 
ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT 
ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC 
ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA 
GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT 
CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA 
GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA 
ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT 
GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC 
CTTCTTGATA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nalcn ( XM_012957712.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813166.1 Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA.

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CloneID	OXa35339
Clone ID Related Accession (Same CDS sequence)	XM_012957712.2
Accession Version	XM_012957712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAAC GAAAGCAAAG CTCAAGAGTG GAAGCACAGC CAGTCACAGA CTTTGGCCCC 
GATGAATCTC TGTCAGACAA TGCTGATATC CTGTGGATTA ACAAACCATG GGTTCACTCT 
TTACTGCGCA TCTGCGCAAT CATTAGTGTT ATTTCTGTTT GTATGAACAC CCCAAAGACA 
TTTGAACATT ACCCACCCCT CCAGTATGTC ACGTTTGCAC TCGATACCCT ACTTATGTTT 
CTTTACACCG CAGAAATGAT TGCAAAAATG CACATAAGAG GCATTGTAAA GGGTGATACT 
TCCTATGTAA AGGATCGCTG GTGCGTCTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCATTGGTGT TGCAGGTATT TGAAATCGCC AAAATCGTTG ACCAAATGTC CCCATGGGGC 
ATGCTGCGGA TCCCACGACC TCTGATAATG ATTCGTGCCT TCCGTATATA CTTCCGCTTT 
GAACTCCCAA GAACGAGGAT AACAAATATC TTGAAGCGTT CAGGAGAGCA AATTTGGAGT 
GTTTCCATAT TTTTATTATT CTTTCTTCTA TTATACGGGA TTCTCGGAGT TCAGATGTTT 
GGAACATTTA CCTTCCATTG TGTTACAAAT GACACAGTAC ATGGAAATCT GACTTGGAAT 
AATTTAGCCA TTCCAGATAC TCACTGCTCC CCTGAAGGTG AAGGATACCA GTGTCCTCGT 
GGTTTTAGGT GCGTGGATCT GAAAGAACTT GGACTGAGCA GGCAAGAGCT TGGCTACAGT 
GGCTTTAATG AAATAGGAAC CAGCATATTT ACGGTTTATG AAGCTGCATC TCAAGAAGGA 
TGGGTTTTTC TTATGTATAG AGCAATAGAC AGCTTCCCCA GATGGCGATC GTACTTCTAT 
TTTATTACCT TAATTTTCTT CCTTGCCTGG CTTGTCAAGA ATGTCTTCAT TGCTGTAATT 
ATTGAAACAT TTGCTGAAAT TCGAGTGCAG TTTCAACAGA TGTGGGGTTC TCGAAGTAGT 
ACTACCTCAA CAGCTACCAC ACAGATGTTT CACGAGGATG CAGCAGGGGG CTGGCAGCTA 
GTGGCAGTTG ATGTGAACAA GCCCCAAGGC AGAGCACCAG CTTGTCTACA GAAAATGATG 
AGATCTTCCA TTTTCCACAT GTTTATTCTG AGCATGGTTG CTGTGGATGT AATTGTTGCT 
GCCAGCAATT ACTACAAAGG AGAGAATTTT AAAAGACAAT ATGATGAGTT TTATCTGGCC 
GAGGTGGCAT TCACCATGCT GTTTGATTTG GAAGCTCTTT TAAAGATTTG GTGCCTGGGC 
TTTACAGGAT ATATCAGTTC ATCACTACAC AAATTTGAGC TTTTGCTTGT AGTTGGAACT 
ACTCTTCATA TTTATCCTGA TCTTTACCAT TCACAGTTTA CATACTTTCA GGTTCTAAGG 
GTGGTGCGAC TGATTAAGAT TTCTCCTGCC TTAGAAGACT TTGTATATAA AATATTTGGA 
CCTGGCAAGA AATTAGGAAG TCTTGTGGTT TTCACCGCTA GCCTATTAAT TGTAATGTCT 
GCCATCAGTT TACAGATGTT CTGCTTTGTG GAAGAGCTGG ACCGTTTTAC AACATTTCCA 
AGGGCATTTA TGTCAATGTT TCAGATTCTG ACACAGGAAG GATGGGTGGA TGTCATGGAT 
CAAACACTTA ATGCCGTTGG GCATATGTGG GCACCAGTTG TTGCAATTTA CTTCATACTT 
TATCATCTTT TTGCTACTTT GATATTGCTT AGTTTGTTTG TGGCTGTTAT CCTGGACAAT 
CTGGAGCTTG ATGAAGACTT GAAAAAGCTT AAACAGTTGA AACAAAGTGA AGCAAATGCC 
GACACCAAAG AAAAATTACC ACTACGCCTA CGTATCTTTG AGAAATTCCC CAACAGACCG 
CAAATGGTGA AGATTTCCAA GCTTCCTTCT GATTTCACAG TTCCAAAAAT AAGGGAGAGT 
TTCATGAAGC AGTTTATTGA CCGCCAGCAA CAAGACACAA GCTGTTTATT AAGAAGGCTT 
CCATCTGCTT CTTCTTCTTC ATGTGATCAT TCCAAACGAT CAGGAGTAGA AGAGAATAAG 
TACATTGACC AAAAGCTTCG TAAATCAGTG TTTAGTATAA GAGCAAGGAA TCTTCTGGAA 
AAGGAAACTG CAGTTAATAA AATCCTCAGA GATTGCACAA GACAGCGCAT GCTGAGTGGA 
TCCTTTGAAG GACAGCCTGC AAAGGAGAGA TCAATACTGA GTGTGCAGCA CCACATAAGA 
CAAGAACGGA GATCACTAAG ACATGGATCA AACAGTCAGA GAATCAGCAG GGGGAAGTCT 
CTGGAAACAC TGACACAAGA CCACTCCAAT ACAGTCAGAT ACAGAAATGC ACAGAGGGAA 
GACAGTGAGA TCAAGATGAT CCAGGAAAAA AAAGAACAAG CAGAAATGAA AAGGAAAGTG 
CAGGAAGAAG AGTTAAGAGA AAACCACCCA TACTTTGATA AGCCACTGTT TATAGTTGGC 
CGTGAACACC GGTTCCGAAA CTTCTGCAGG GTAATAGTTC GGGCACGATT TAATGCCTCA 
AAGACAGATC CTGTTACAGG AGCAGTGAAA AACACCAAAT ATCATCAACT TTATGATTTG 
TTGGGACTAG TGACCTATCT AGACTGGGTT ATGATTGTTG TTACCATATG TTCCTGCATA 
TCAATGATGT TTGAATCTCC TTTTAGAAGA GTAATGCACA CCCCCACGTT ACAGATTGCA 
GAATATGTTT TTGTCATATT CATGAGCATT GAACTGAACT TGAAGATCAT GGCAGATGGC 
TTATTTTTTA CTCCAACGGC TGTTATACGA GACTTTGGGG GTGTTATGGA TATCTTCATT 
TATCTTGTTA GTTTAATCTT CTTATGCTGG ATGCCTAATT ATGTTCCCGC AAAATCTGGA 
ACCCAACTAT TAATGGTGCT GCGCTGCTTG AGACCACTGA GAATATTCAA GCTTGTACCA 
CAGATGAGGA AAGTCGTACG GGAACTTTTC AGTGGGTTTA AAGAAATATT TCTGGTTTCT 
ATTCTTCTGC TGACACTGAT GCTTGTGTTT GCAAGCTTTG GCGTGCAGCT CTTTGCAGGG 
AAACTGGCAA AGTGCAATGA CCCACACATA CTCACCAGGG AAGACTGTCA TGGAATATTT 
AGAATTAATG TCAGTGTTTC GAAAAATTTA AATCTGAAAT TAAAACCAGA GGAAAAGAAG 
CCTGGATTCT GGGTTCCAAG AGTCTGGGCA AATCCCCGTA ATTTTAATTT TGACAATGTA 
GGAAATGCCA TGCTGGCCTT GTTTGAGGTA TTGTCTTTGA AGGGCTGGGT TGAAGTGCGA 
GATGTTATTA TTCATCGAGT TGGACCGATC CATGGAATTT ATATTCATGT CTTTGTGTTT 
CTTGGCTGTA TGATTGGCTT GACTCTCTTT GTTGGTGTGG TTATTGCTAA CTTTAATGAG 
AATAAGGGTA CAGCTTTACT GACTGTTGAT CAAAGGAGAT GGGAAGATCT GAAAAGCAGA 
CTTAAGATAG CCCAACCACT TCATCTCCCC CCACGTCCAG ATAATGATGG TTTCAGAGCC 
CGCATGTATG ACATAACTCA ACACCCTTTT TTCAAGAGGA CTATTGCTGT ACTTGTTTTA 
GCACAGTCTG TGCTTCTGTC AGTAAAGTGG GATGTTGAAG ACCCTGTGAC GGTTCCACTG 
GCAACTATGT CTGTGGTTTT CACCTTCATC TTTGTACTTG AGGTAACCAT GAAGATAACA 
GCTATGTCAC CAGCTGGATT TTGGCAAAGT AGAAGAAATC GCTATGATCT CCTTGTCACA 
TCTCTTGGTG TGATCTGGGT GATACTTCAT TTTGCTTTAC TGAATGAATA CACTTATATG 
ATGGGTGCTT GTATTATCGT CTTTCGTTTT TTCTCTATCT GTGGCAAACA TGTCACACTG 
AAAATGTTAT TATTGACTGT GGTAGTCAGC ATGTACAAGA GCTTCTTCAT AATAGTAGGA 
ATGTTTCTGC TGCTTCTCTG CTATGCTTTT GCCGGTGTTG TTCTGTTTGG AACAGTGAAA 
TATGGAGAAA ACATAAACAG GCATGCTAAC TTTTCCACAG CAGGGAAAGC TATAACTGTG 
CTATTCAGAA TAGTAACTGG AGAAGACTGG AACAAAATAA TGCATGACTG CATGGTACAG 
CCTCCTTTTT GCACTCCAGA TAACAGCAAA TATTGGGAAA CAGATTGTGG AAATTATGCA 
GGGGCACTTA TGTACTTCTG TTCATTTTAT GTCATCATTG CTTACATCAT GTTAAATCTA 
CTGGTTGCCA TAATTGTGGA AAACTTTTCC CTCTTCTACT CTACTGAAGA AGACCAGCTC 
CTAAGCCCTA ATGATCTGCG CCATTACCAA ATAATATGGA ATATGGTTGA TGATAAAAGA 
GAGGGGGTTA TTCCAGCTTT TCGTGTGAAG TTCCTTCTGA GACTGCTACG CGGGAGGCTG 
GAGGTTGACT TAGATAAGGA CAAGCTTTTG TTCAAACACA TGTGCTATGA AATGGAGAGA 
CTGCACAGCG GGGGAGATGT CACTTTCCAT GATGTACTTA GCATGCTTTC CTACAGATCA 
GTGGATATCA GAAAGAGCCT TCAGCTGGAG GAGTTGCTAG CCAGAGAACA GCTAGAATAC 
ACAATAGAAG AAGAAGTAGC TAAACAAACA ATACGAATGT GGTTGAAAAA GTGTTTGAAA 
CGCATTCGAG CAAAACAACA GCAATCATGC AGTATTATAC ACAGTTTGAG AGAGAGCCAA 
CAGCAGGAAC TGAGCAGGTT TCTGAACCCT CCTAGCATTG AGACCACACA GCCAAGTGAA 
GATATGAACC TAAGCAACCA AGACAATGCA CAGCCAGAGA CAAGTAGCCA ACAACAGCTT 
CTTAGCCCAA CGCTTTCTGA CAGAGGAGGT TATCGTCAGG ATTCAATTGA AGCTGGTAAA 
CCTCAGAGAA AGTTTGGGCA GTGGCGATTA CCAGCAGCAC CAAAACCAAT AAGCCATTCT 
GTGTCCTCTG TGAACCTACG ATTTGGAGGA CGGTCAACAA TGAAGTCTGT AGTGTGCAAG 
ATGAACGCCA TGTCTGATGC TGCCTCCTGT GGTTCTGAAG TCAAGAAATG GTGGACAAGA 
CAACTGACAG TAGAAAGTGA TGAGAGTGGT GATGACCTTC TTGATATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813166.1
CDS	625..5835
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVKGDTSYVKDRWCVFDGFMVFCLWVSLVLQVFEIAKIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTFHCVTNDTVHGNLTWNNLAIPDTHCSPEGEGYQCPRGFRCVDLKELGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSIFHMFILSMVAVDVIVAASNYYKGENFKRQYDEFYLAEVAFTMLFDLEALLKIWCLGFTGYISSSLHKFELLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTSCLLRRLPSASSSSCDHSKRSGVEENKYIDQKLRKSVFSIRARNLLEKETAVNKILRDCTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVIVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFRRVMHTPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPNYVPAKSGTQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPHILTREDCHGIFRINVSVSKNLNLKLKPEEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRARMYDITQHPFFKRTIAVLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKITAMSPAGFWQSRRNRYDLLVTSLGVIWVILHFALLNEYTYMMGACIIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDNSKYWETDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSPNDLRHYQIIWNMVDDKREGVIPAFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHSGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDMNLSNQDNAQPETSSQQQLLSPTLSDRGGYRQDSIEAGKPQRKFGQWRLPAAPKPISHSVSSVNLRFGGRSTMKSVVCKMNAMSDAASCGSEVKKWWTRQLTVESDESGDDLLDM

Target ORF information:

RefSeq Version	XM_012957712.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957712.2

ORF Insert Sequence:

ATGTTGAAAC GAAAGCAAAG CTCAAGAGTG GAAGCACAGC CAGTCACAGA CTTTGGCCCC 
GATGAATCTC TGTCAGACAA TGCTGATATC CTGTGGATTA ACAAACCATG GGTTCACTCT 
TTACTGCGCA TCTGCGCAAT CATTAGTGTT ATTTCTGTTT GTATGAACAC CCCAAAGACA 
TTTGAACATT ACCCACCCCT CCAGTATGTC ACGTTTGCAC TCGATACCCT ACTTATGTTT 
CTTTACACCG CAGAAATGAT TGCAAAAATG CACATAAGAG GCATTGTAAA GGGTGATACT 
TCCTATGTAA AGGATCGCTG GTGCGTCTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA 
TCATTGGTGT TGCAGGTATT TGAAATCGCC AAAATCGTTG ACCAAATGTC CCCATGGGGC 
ATGCTGCGGA TCCCACGACC TCTGATAATG ATTCGTGCCT TCCGTATATA CTTCCGCTTT 
GAACTCCCAA GAACGAGGAT AACAAATATC TTGAAGCGTT CAGGAGAGCA AATTTGGAGT 
GTTTCCATAT TTTTATTATT CTTTCTTCTA TTATACGGGA TTCTCGGAGT TCAGATGTTT 
GGAACATTTA CCTTCCATTG TGTTACAAAT GACACAGTAC ATGGAAATCT GACTTGGAAT 
AATTTAGCCA TTCCAGATAC TCACTGCTCC CCTGAAGGTG AAGGATACCA GTGTCCTCGT 
GGTTTTAGGT GCGTGGATCT GAAAGAACTT GGACTGAGCA GGCAAGAGCT TGGCTACAGT 
GGCTTTAATG AAATAGGAAC CAGCATATTT ACGGTTTATG AAGCTGCATC TCAAGAAGGA 
TGGGTTTTTC TTATGTATAG AGCAATAGAC AGCTTCCCCA GATGGCGATC GTACTTCTAT 
TTTATTACCT TAATTTTCTT CCTTGCCTGG CTTGTCAAGA ATGTCTTCAT TGCTGTAATT 
ATTGAAACAT TTGCTGAAAT TCGAGTGCAG TTTCAACAGA TGTGGGGTTC TCGAAGTAGT 
ACTACCTCAA CAGCTACCAC ACAGATGTTT CACGAGGATG CAGCAGGGGG CTGGCAGCTA 
GTGGCAGTTG ATGTGAACAA GCCCCAAGGC AGAGCACCAG CTTGTCTACA GAAAATGATG 
AGATCTTCCA TTTTCCACAT GTTTATTCTG AGCATGGTTG CTGTGGATGT AATTGTTGCT 
GCCAGCAATT ACTACAAAGG AGAGAATTTT AAAAGACAAT ATGATGAGTT TTATCTGGCC 
GAGGTGGCAT TCACCATGCT GTTTGATTTG GAAGCTCTTT TAAAGATTTG GTGCCTGGGC 
TTTACAGGAT ATATCAGTTC ATCACTACAC AAATTTGAGC TTTTGCTTGT AGTTGGAACT 
ACTCTTCATA TTTATCCTGA TCTTTACCAT TCACAGTTTA CATACTTTCA GGTTCTAAGG 
GTGGTGCGAC TGATTAAGAT TTCTCCTGCC TTAGAAGACT TTGTATATAA AATATTTGGA 
CCTGGCAAGA AATTAGGAAG TCTTGTGGTT TTCACCGCTA GCCTATTAAT TGTAATGTCT 
GCCATCAGTT TACAGATGTT CTGCTTTGTG GAAGAGCTGG ACCGTTTTAC AACATTTCCA 
AGGGCATTTA TGTCAATGTT TCAGATTCTG ACACAGGAAG GATGGGTGGA TGTCATGGAT 
CAAACACTTA ATGCCGTTGG GCATATGTGG GCACCAGTTG TTGCAATTTA CTTCATACTT 
TATCATCTTT TTGCTACTTT GATATTGCTT AGTTTGTTTG TGGCTGTTAT CCTGGACAAT 
CTGGAGCTTG ATGAAGACTT GAAAAAGCTT AAACAGTTGA AACAAAGTGA AGCAAATGCC 
GACACCAAAG AAAAATTACC ACTACGCCTA CGTATCTTTG AGAAATTCCC CAACAGACCG 
CAAATGGTGA AGATTTCCAA GCTTCCTTCT GATTTCACAG TTCCAAAAAT AAGGGAGAGT 
TTCATGAAGC AGTTTATTGA CCGCCAGCAA CAAGACACAA GCTGTTTATT AAGAAGGCTT 
CCATCTGCTT CTTCTTCTTC ATGTGATCAT TCCAAACGAT CAGGAGTAGA AGAGAATAAG 
TACATTGACC AAAAGCTTCG TAAATCAGTG TTTAGTATAA GAGCAAGGAA TCTTCTGGAA 
AAGGAAACTG CAGTTAATAA AATCCTCAGA GATTGCACAA GACAGCGCAT GCTGAGTGGA 
TCCTTTGAAG GACAGCCTGC AAAGGAGAGA TCAATACTGA GTGTGCAGCA CCACATAAGA 
CAAGAACGGA GATCACTAAG ACATGGATCA AACAGTCAGA GAATCAGCAG GGGGAAGTCT 
CTGGAAACAC TGACACAAGA CCACTCCAAT ACAGTCAGAT ACAGAAATGC ACAGAGGGAA 
GACAGTGAGA TCAAGATGAT CCAGGAAAAA AAAGAACAAG CAGAAATGAA AAGGAAAGTG 
CAGGAAGAAG AGTTAAGAGA AAACCACCCA TACTTTGATA AGCCACTGTT TATAGTTGGC 
CGTGAACACC GGTTCCGAAA CTTCTGCAGG GTAATAGTTC GGGCACGATT TAATGCCTCA 
AAGACAGATC CTGTTACAGG AGCAGTGAAA AACACCAAAT ATCATCAACT TTATGATTTG 
TTGGGACTAG TGACCTATCT AGACTGGGTT ATGATTGTTG TTACCATATG TTCCTGCATA 
TCAATGATGT TTGAATCTCC TTTTAGAAGA GTAATGCACA CCCCCACGTT ACAGATTGCA 
GAATATGTTT TTGTCATATT CATGAGCATT GAACTGAACT TGAAGATCAT GGCAGATGGC 
TTATTTTTTA CTCCAACGGC TGTTATACGA GACTTTGGGG GTGTTATGGA TATCTTCATT 
TATCTTGTTA GTTTAATCTT CTTATGCTGG ATGCCTAATT ATGTTCCCGC AAAATCTGGA 
ACCCAACTAT TAATGGTGCT GCGCTGCTTG AGACCACTGA GAATATTCAA GCTTGTACCA 
CAGATGAGGA AAGTCGTACG GGAACTTTTC AGTGGGTTTA AAGAAATATT TCTGGTTTCT 
ATTCTTCTGC TGACACTGAT GCTTGTGTTT GCAAGCTTTG GCGTGCAGCT CTTTGCAGGG 
AAACTGGCAA AGTGCAATGA CCCACACATA CTCACCAGGG AAGACTGTCA TGGAATATTT 
AGAATTAATG TCAGTGTTTC GAAAAATTTA AATCTGAAAT TAAAACCAGA GGAAAAGAAG 
CCTGGATTCT GGGTTCCAAG AGTCTGGGCA AATCCCCGTA ATTTTAATTT TGACAATGTA 
GGAAATGCCA TGCTGGCCTT GTTTGAGGTA TTGTCTTTGA AGGGCTGGGT TGAAGTGCGA 
GATGTTATTA TTCATCGAGT TGGACCGATC CATGGAATTT ATATTCATGT CTTTGTGTTT 
CTTGGCTGTA TGATTGGCTT GACTCTCTTT GTTGGTGTGG TTATTGCTAA CTTTAATGAG 
AATAAGGGTA CAGCTTTACT GACTGTTGAT CAAAGGAGAT GGGAAGATCT GAAAAGCAGA 
CTTAAGATAG CCCAACCACT TCATCTCCCC CCACGTCCAG ATAATGATGG TTTCAGAGCC 
CGCATGTATG ACATAACTCA ACACCCTTTT TTCAAGAGGA CTATTGCTGT ACTTGTTTTA 
GCACAGTCTG TGCTTCTGTC AGTAAAGTGG GATGTTGAAG ACCCTGTGAC GGTTCCACTG 
GCAACTATGT CTGTGGTTTT CACCTTCATC TTTGTACTTG AGGTAACCAT GAAGATAACA 
GCTATGTCAC CAGCTGGATT TTGGCAAAGT AGAAGAAATC GCTATGATCT CCTTGTCACA 
TCTCTTGGTG TGATCTGGGT GATACTTCAT TTTGCTTTAC TGAATGAATA CACTTATATG 
ATGGGTGCTT GTATTATCGT CTTTCGTTTT TTCTCTATCT GTGGCAAACA TGTCACACTG 
AAAATGTTAT TATTGACTGT GGTAGTCAGC ATGTACAAGA GCTTCTTCAT AATAGTAGGA 
ATGTTTCTGC TGCTTCTCTG CTATGCTTTT GCCGGTGTTG TTCTGTTTGG AACAGTGAAA 
TATGGAGAAA ACATAAACAG GCATGCTAAC TTTTCCACAG CAGGGAAAGC TATAACTGTG 
CTATTCAGAA TAGTAACTGG AGAAGACTGG AACAAAATAA TGCATGACTG CATGGTACAG 
CCTCCTTTTT GCACTCCAGA TAACAGCAAA TATTGGGAAA CAGATTGTGG AAATTATGCA 
GGGGCACTTA TGTACTTCTG TTCATTTTAT GTCATCATTG CTTACATCAT GTTAAATCTA 
CTGGTTGCCA TAATTGTGGA AAACTTTTCC CTCTTCTACT CTACTGAAGA AGACCAGCTC 
CTAAGCCCTA ATGATCTGCG CCATTACCAA ATAATATGGA ATATGGTTGA TGATAAAAGA 
GAGGGGGTTA TTCCAGCTTT TCGTGTGAAG TTCCTTCTGA GACTGCTACG CGGGAGGCTG 
GAGGTTGACT TAGATAAGGA CAAGCTTTTG TTCAAACACA TGTGCTATGA AATGGAGAGA 
CTGCACAGCG GGGGAGATGT CACTTTCCAT GATGTACTTA GCATGCTTTC CTACAGATCA 
GTGGATATCA GAAAGAGCCT TCAGCTGGAG GAGTTGCTAG CCAGAGAACA GCTAGAATAC 
ACAATAGAAG AAGAAGTAGC TAAACAAACA ATACGAATGT GGTTGAAAAA GTGTTTGAAA 
CGCATTCGAG CAAAACAACA GCAATCATGC AGTATTATAC ACAGTTTGAG AGAGAGCCAA 
CAGCAGGAAC TGAGCAGGTT TCTGAACCCT CCTAGCATTG AGACCACACA GCCAAGTGAA 
GATATGAACC TAAGCAACCA AGACAATGCA CAGCCAGAGA CAAGTAGCCA ACAACAGCTT 
CTTAGCCCAA CGCTTTCTGA CAGAGGAGGT TATCGTCAGG ATTCAATTGA AGCTGGTAAA 
CCTCAGAGAA AGTTTGGGCA GTGGCGATTA CCAGCAGCAC CAAAACCAAT AAGCCATTCT 
GTGTCCTCTG TGAACCTACG ATTTGGAGGA CGGTCAACAA TGAAGTCTGT AGTGTGCAAG 
ATGAACGCCA TGTCTGATGC TGCCTCCTGT GGTTCTGAAG TCAAGAAATG GTGGACAAGA 
CAACTGACAG TAGAAAGTGA TGAGAGTGGT GATGACCTTC TTGATATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

nalcn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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