HEPH gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005228266.3	XP_005228323.1	hephaestin isoform X1
XM_002700167.5	XP_002700213.3	hephaestin isoform X2
XM_015470499.1	XP_015325985.1	hephaestin isoform X2
XM_015470504.1	XP_015325990.1	hephaestin isoform X6
XM_005228266.4	XP_005228323.1	hephaestin isoform X1
XM_002700167.6	XP_002700213.3	hephaestin isoform X2
XM_015461727.2	XP_015317213.1	hephaestin isoform X3
XM_005228269.4	XP_005228326.1	hephaestin isoform X5
XM_005228269.3	XP_005228326.1	hephaestin isoform X5
XM_005228270.3	XP_005228327.1	hephaestin isoform X6
XM_010822045.3	XP_010820347.1	hephaestin isoform X7
XM_015461727.1	XP_015317213.1	hephaestin isoform X3
XM_010822042.2	XP_010820344.1	hephaestin isoform X3
XM_010822043.2	XP_010820345.1	hephaestin isoform X4
XM_015470498.1	XP_015325984.1	hephaestin isoform X1
XM_015470500.1	XP_015325986.1	hephaestin isoform X3
XM_015470501.1	XP_015325987.1	hephaestin isoform X3
XM_015470502.1	XP_015325988.1	hephaestin isoform X4
XM_015470505.1	XP_015325991.1	hephaestin isoform X7
XM_010822043.3	XP_010820345.1	hephaestin isoform X4
XM_005228270.4	XP_005228327.1	hephaestin isoform X6
XM_015470503.1	XP_015325989.1	hephaestin isoform X5
XM_010822045.2	XP_010820347.1	hephaestin isoform X7
XM_010822042.3	XP_010820344.1	hephaestin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa35673	XM_005228270.4 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa35673	XM_015470504.1 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa47792	XM_015470505.1 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa47792	XM_010822045.3 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa35668	XM_005228266.4 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35668	XM_015470498.1 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35669	XM_015470499.1 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35669	XM_002700167.6 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35671	XM_015470500.1 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35671	XM_015470501.1 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35671	XM_015461727.2 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35671	XM_010822042.3 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35672	XM_015470503.1 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35672	XM_005228269.4 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa47790	XM_010822043.3 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa47790	XM_015470502.1 Latest version!	Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa35668	XM_005228266.3	Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35669	XM_002700167.5	Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35672	XM_005228269.3	Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35671	XM_015461727.1	Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa47790	XM_010822043.2	Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35673	XM_005228270.3	Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa47792	XM_010822045.2	Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa35671	XM_010822042.2	Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

HEPH ( XM_015470504.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325990.1 Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa35673
Clone ID Related Accession (Same CDS sequence)	XM_015470504.1 , XM_005228270.3 , XM_005228270.4
Accession Version	XM_015470504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA 
GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT 
TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG 
GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325990.1
CDS	268..3177
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPEHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015470504.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470504.1

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA 
GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT 
TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG 
GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_005228270.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228327.1 Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa35673
Clone ID Related Accession (Same CDS sequence)	XM_015470504.1 , XM_005228270.3 , XM_005228270.4
Accession Version	XM_005228270.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228270.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA 
GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT 
TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG 
GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228327.1
CDS	268..3177
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPEHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_005228270.3
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228270.3

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA 
GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT 
TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG 
GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_005228270.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228327.1 Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa35673
Clone ID Related Accession (Same CDS sequence)	XM_015470504.1 , XM_005228270.3 , XM_005228270.4
Accession Version	XM_005228270.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hephaestin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228270.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA 
GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT 
TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG 
GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228327.1
CDS	271..3180
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPEHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_005228270.4
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228270.4

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA 
GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT 
TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG 
GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_010822045.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820347.1 Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa47792
Clone ID Related Accession (Same CDS sequence)	XM_010822045.3 , XM_015470505.1 , XM_010822045.2
Accession Version	XM_010822045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hephaestin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010822045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA 
GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT 
GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG 
TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC 
TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820347.1
CDS	271..3177
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPEHLSTTTTIPEEIGKAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_010822045.3
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822045.3

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA 
GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT 
GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG 
TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC 
TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015470505.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325991.1 Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa47792
Clone ID Related Accession (Same CDS sequence)	XM_010822045.3 , XM_015470505.1 , XM_010822045.2
Accession Version	XM_015470505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA 
GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT 
GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG 
TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC 
TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325991.1
CDS	269..3175
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPEHLSTTTTIPEEIGKAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015470505.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470505.1

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA 
GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT 
GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG 
TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC 
TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_010822045.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820347.1 Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa47792
Clone ID Related Accession (Same CDS sequence)	XM_010822045.3 , XM_015470505.1 , XM_010822045.2
Accession Version	XM_010822045.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA 
GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT 
GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG 
TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC 
TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820347.1
CDS	269..3175
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPEHLSTTTTIPEEIGKAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_010822045.2
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822045.2

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA 
AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA 
GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT 
GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG 
TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC 
TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_005228266.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228323.1 Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

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CloneID	OBa35668
Clone ID Related Accession (Same CDS sequence)	XM_005228266.3 , XM_005228266.4 , XM_015470498.1
Accession Version	XM_005228266.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228266.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC 
ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC 
ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG 
AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228323.1
CDS	265..3810
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_005228266.3
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228266.3

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC 
ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC 
ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG 
AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_005228266.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228323.1 Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35668
Clone ID Related Accession (Same CDS sequence)	XM_005228266.3 , XM_005228266.4 , XM_015470498.1
Accession Version	XM_005228266.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228266.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC 
ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC 
ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG 
AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228323.1
CDS	263..3808
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_005228266.4
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228266.4

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC 
ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC 
ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG 
AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015470498.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325984.1 Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35668
Clone ID Related Accession (Same CDS sequence)	XM_005228266.3 , XM_005228266.4 , XM_015470498.1
Accession Version	XM_015470498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC 
ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC 
ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG 
AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325984.1
CDS	265..3810
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015470498.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470498.1

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC 
ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC 
ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG 
AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_002700167.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700213.3 Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

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CloneID	OBa35669
Clone ID Related Accession (Same CDS sequence)	XM_002700167.5 , XM_015470499.1 , XM_002700167.6
Accession Version	XM_002700167.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700167.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG 
CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT 
CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG 
TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700213.3
CDS	265..3807
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_002700167.5
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700167.5

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG 
CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT 
CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG 
TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015470499.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325985.1 Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35669
Clone ID Related Accession (Same CDS sequence)	XM_002700167.5 , XM_015470499.1 , XM_002700167.6
Accession Version	XM_015470499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG 
CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT 
CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG 
TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325985.1
CDS	265..3807
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015470499.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470499.1

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG 
CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT 
CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG 
TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_002700167.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700213.3 Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

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CloneID	OBa35669
Clone ID Related Accession (Same CDS sequence)	XM_002700167.5 , XM_015470499.1 , XM_002700167.6
Accession Version	XM_002700167.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hephaestin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002700167.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG 
CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT 
CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG 
TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700213.3
CDS	268..3810
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_002700167.6
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700167.6

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG 
GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC 
TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT 
TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT 
TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC 
ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG 
GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA 
CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG 
GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT 
CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT 
GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG 
GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG 
AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG 
GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT 
TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC 
CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT 
GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC 
CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG 
ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA 
GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG 
CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT 
CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG 
TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015461727.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317213.1 Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35671
Clone ID Related Accession (Same CDS sequence)	XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
Accession Version	XM_015461727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hephaestin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317213.1
CDS	89..3565
Translation	MESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015461727.2
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461727.2

ORF Insert Sequence:

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015461727.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317213.1 Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35671
Clone ID Related Accession (Same CDS sequence)	XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
Accession Version	XM_015461727.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317213.1
CDS	101..3577
Translation	MESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015461727.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461727.1

ORF Insert Sequence:

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_010822042.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820344.1 Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

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CloneID	OBa35671
Clone ID Related Accession (Same CDS sequence)	XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
Accession Version	XM_010822042.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820344.1
CDS	308..3784
Translation	MESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_010822042.2
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822042.2

ORF Insert Sequence:

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015470500.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325986.1 Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35671
Clone ID Related Accession (Same CDS sequence)	XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
Accession Version	XM_015470500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325986.1
CDS	101..3577
Translation	MESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015470500.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470500.1

ORF Insert Sequence:

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015470501.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325987.1 Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa35671
Clone ID Related Accession (Same CDS sequence)	XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
Accession Version	XM_015470501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325987.1
CDS	308..3784
Translation	MESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015470501.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470501.1

ORF Insert Sequence:

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_010822042.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820344.1 Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

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CloneID	OBa35671
Clone ID Related Accession (Same CDS sequence)	XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
Accession Version	XM_010822042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hephaestin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010822042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820344.1
CDS	310..3786
Translation	MESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_010822042.3
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822042.3

ORF Insert Sequence:

ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT 
CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC 
TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA 
TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG 
CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT 
CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG 
TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC 
CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC 
CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT 
GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG 
GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT 
ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG 
GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA 
GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT 
GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT 
GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC 
TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG 
CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG 
AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC 
TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA 
TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC 
CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT 
GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA 
GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC 
TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC 
AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC 
CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT 
ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG 
CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC 
ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC 
CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC 
ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA 
GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA 
CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA 
GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT 
GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA 
TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG 
ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT 
ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA 
GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC 
CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG 
ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG 
GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA 
TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG 
GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG 
AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC 
ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT 
GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT 
ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA 
AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC 
CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG 
CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG 
CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_005228269.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228326.1 Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

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CloneID	OBa35672
Clone ID Related Accession (Same CDS sequence)	XM_005228269.4 , XM_005228269.3 , XM_015470503.1
Accession Version	XM_005228269.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hephaestin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228269.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT 
CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT 
GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT 
TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT 
AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC 
TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC 
ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT 
AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT 
TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT 
CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC 
TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG 
AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC 
ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG 
CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA 
GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC 
TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT 
CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA 
AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT 
ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG 
CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC 
ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT 
CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT 
TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC 
AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC 
TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC 
AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT 
GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG 
AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC 
ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG 
ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG 
GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC 
ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA 
GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA 
GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC 
CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA 
GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA 
AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG 
ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT 
GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228326.1
CDS	387..3605
Translation	MDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_005228269.4
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228269.4

ORF Insert Sequence:

ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT 
CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT 
GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT 
TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT 
AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC 
TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC 
ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT 
AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT 
TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT 
CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC 
TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG 
AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC 
ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG 
CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA 
GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC 
TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT 
CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA 
AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT 
ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG 
CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC 
ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT 
CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT 
TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC 
AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC 
TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC 
AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT 
GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG 
AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC 
ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG 
ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG 
GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC 
ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA 
GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA 
GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC 
CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA 
GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA 
AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG 
ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT 
GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_005228269.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228326.1 Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

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CloneID	OBa35672
Clone ID Related Accession (Same CDS sequence)	XM_005228269.4 , XM_005228269.3 , XM_015470503.1
Accession Version	XM_005228269.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228269.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT 
CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT 
GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT 
TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT 
AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC 
TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC 
ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT 
AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT 
TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT 
CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC 
TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG 
AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC 
ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG 
CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA 
GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC 
TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT 
CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA 
AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT 
ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG 
CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC 
ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT 
CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT 
TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC 
AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC 
TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC 
AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT 
GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG 
AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC 
ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG 
ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG 
GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC 
ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA 
GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA 
GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC 
CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA 
GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA 
AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG 
ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT 
GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228326.1
CDS	385..3603
Translation	MDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_005228269.3
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228269.3

ORF Insert Sequence:

ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT 
CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT 
GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT 
TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT 
AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC 
TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC 
ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT 
AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT 
TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT 
CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC 
TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG 
AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC 
ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG 
CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA 
GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC 
TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT 
CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA 
AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT 
ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG 
CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC 
ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT 
CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT 
TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC 
AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC 
TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC 
AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT 
GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG 
AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC 
ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG 
ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG 
GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC 
ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA 
GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA 
GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC 
CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA 
GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA 
AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG 
ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT 
GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015470503.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325989.1 Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa35672
Clone ID Related Accession (Same CDS sequence)	XM_005228269.4 , XM_005228269.3 , XM_015470503.1
Accession Version	XM_015470503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT 
CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT 
GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT 
TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT 
AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC 
TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC 
ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT 
AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT 
TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT 
CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC 
TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG 
AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC 
ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG 
CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA 
GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC 
TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT 
CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA 
AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT 
ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG 
CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC 
ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT 
CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT 
TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC 
AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC 
TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC 
AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT 
GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG 
AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC 
ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG 
ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG 
GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC 
ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA 
GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA 
GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC 
CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA 
GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA 
AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG 
ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT 
GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325989.1
CDS	385..3603
Translation	MDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHAINGFLFSNLPKLDMCKGDTVAWHLLGLGTETDVHGVTFQGNTVQLQGMRKSAATLFPHTFVMAIMQPDNPGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015470503.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470503.1

ORF Insert Sequence:

ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT 
CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT 
GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT 
TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT 
AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC 
TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC 
ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT 
AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT 
TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT 
CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC 
TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG 
AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC 
ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG 
CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA 
GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC 
TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT 
CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA 
AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT 
ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG 
CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC 
ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT 
CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT 
TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC 
AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC 
TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC 
AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT 
GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG 
AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC 
ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG 
ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG 
GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC 
ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA 
GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA 
GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC 
CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA 
GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA 
AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG 
ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT 
GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_010822043.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820345.1 Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa47790
Clone ID Related Accession (Same CDS sequence)	XM_010822043.2 , XM_015470502.1 , XM_010822043.3
Accession Version	XM_010822043.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT 
TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA 
GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC 
TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG 
TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA 
ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC 
AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT 
GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG 
GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT 
GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA 
GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT 
GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC 
TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT 
ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC 
TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT 
GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG 
ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT 
GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820345.1
CDS	265..3597
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_010822043.2
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822043.2

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT 
TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA 
GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC 
TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG 
TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA 
ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC 
AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT 
GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG 
GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT 
GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA 
GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT 
GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC 
TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT 
ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC 
TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT 
GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG 
ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT 
GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015470502.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325988.1 Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa47790
Clone ID Related Accession (Same CDS sequence)	XM_010822043.2 , XM_015470502.1 , XM_010822043.3
Accession Version	XM_015470502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hephaestin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT 
TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA 
GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC 
TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG 
TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA 
ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC 
AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT 
GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG 
GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT 
GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA 
GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT 
GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC 
TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT 
ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC 
TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT 
GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG 
ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT 
GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325988.1
CDS	265..3597
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_015470502.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470502.1

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT 
TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA 
GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC 
TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG 
TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA 
ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC 
AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT 
GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG 
GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT 
GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA 
GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT 
GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC 
TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT 
ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC 
TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT 
GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG 
ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT 
GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_010822043.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820345.1 Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa47790
Clone ID Related Accession (Same CDS sequence)	XM_010822043.2 , XM_015470502.1 , XM_010822043.3
Accession Version	XM_010822043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hephaestin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010822043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT 
TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA 
GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC 
TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG 
TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA 
ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC 
AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT 
GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG 
GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT 
GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA 
GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT 
GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC 
TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT 
ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC 
TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT 
GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG 
ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT 
GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820345.1
CDS	268..3600
Translation	MNMEGFSPAKKLFICLFPRVTWAMESGHLLWALLFMQPLWPQLTDGTTRVYYLGIQDVRWNYAPKGRNIITNQPLESDIVAASFLKSDKNRIGNSYKKTIYKEYKDGSYMDEMVQPAWLGFLGPVLRAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGWLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWVYHSHVDAPRDIATGLIGPLITCKRGTLDGSFPPRRQDVDNDFFLLFSVIDENLSWHINENIATYCSDPASVDKEDEDFQKSNRMHAINGFVFGNLPDLSMCAQKRVAWHLFGMGNEVDVHTVFFHGQMLTIRGHRTDVAHIFPATFVSAEMVPQEPGTWLISCQVNSHLQDGMQALYKVKSCSMAPPMNLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSGSDKFFQKSSIRIGGTYWKVRYEAFQDETFQEKVQLEEDKHLGILGPVIRAEVGDTILVIFYNRASQPFSIQPHGVFYEKDSEGTVYNDDSSHSGLVARPFEKVTYRWTVPPHAGPTVQDPACLTWTYFSAADPIRDTNSGLVGPLLVCKAGTLGTDGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDSRLLSEDAKGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSLCPGHQANPHQRYQAARIYYIMAEEMEWDYCPDRSWELEWHNQSEKDSYGHIFLSNKDGLLGSRYKKAVFREYTDGTFRVQRPRTGSEEHLGILGPLIRGEVGDILTVVFKNNASRPYSVHAHGVLESSTGWPLAAEPGEVLTYQWNIPERSGPGLNDSACVSWIYYSAVDPIKDMYSGLVGPLAICRKGILEPYGGRRDMNREFALLFLIFDENKSWYLEENVETYGSQDPGRVNLQDETFLESNKMHAINGKLYANLMGLTMYQGERVAWYMMAMGQDIDLHTVHFHAESFLYRSGESYRADVVDLFPGTFEVVEMVASNPGTWLLHCHVTDHVHAGMETVFTVLSQREHLSTTTTIPEEIGKVAIPRNIGEGSVKMLGMQIPIKDIEMLTSVLIAMGVILLLIALVLGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_010822043.3
Organism	Bos taurus(cattle)
Definition	Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822043.3

ORF Insert Sequence:

ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG 
CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG 
AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA 
GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC 
TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA 
GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA 
GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC 
CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT 
GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT 
CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT 
AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA 
GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA 
AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT 
CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC 
CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG 
CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC 
AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG 
CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC 
CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA 
GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG 
TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG 
ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG 
CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT 
AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT 
TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA 
GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC 
TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG 
TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA 
ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC 
AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT 
GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG 
GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT 
GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA 
GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT 
GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC 
TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT 
ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC 
TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT 
GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG 
ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT 
GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HEPH
Entrez Gene ID	510736
Full Name	hephaestin
General protein information	Preferred Names hephaestin Names hephaestin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	HEPH	NP_620074.3
Pan troglodytes (chimpanzee)	HEPH	XP_003954062.1
	HEPH	XP_001099571.2
Bos taurus (cattle)	HEPH	XP_005228323.1
Canis lupus familiaris (dog)	HEPH	XP_005641482.1
Mus musculus (house mouse)	Heph	NP_001153100.1
Rattus norvegicus (Norway rat)	Heph	NP_579838.1
Gallus gallus (chicken)	HEPH	XP_420165.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HEPH cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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