HEPH cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEPH
Entrez Gene ID	422168
Full Name	hephaestin
General protein information	Preferred Names hephaestin Names hephaestin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_420165.6	XP_420165.4	hephaestin isoform X1
XM_004940526.3	XP_004940583.1	hephaestin isoform X1
XM_025150285.1	XP_025006053.1	hephaestin isoform X2
XM_004940526.2	XP_004940583.1	hephaestin
XM_015285254.1	XP_015140740.1	hephaestin
XM_420165.5	XP_420165.4	hephaestin

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEPH	NP_620074.3
Pan troglodytes (chimpanzee)	HEPH	XP_003954062.1
	HEPH	XP_001099571.2
Bos taurus (cattle)	HEPH	XP_005228323.1
Canis lupus familiaris (dog)	HEPH	XP_005641482.1
Mus musculus (house mouse)	Heph	NP_001153100.1
Rattus norvegicus (Norway rat)	Heph	NP_579838.1
Gallus gallus (chicken)	HEPH	XP_420165.4

Gene Ontology

The following HEPH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEPH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa150555	XM_025150285.1 Latest version!	Gallus gallus hephaestin (HEPH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa07206	XM_004940526.3 Latest version!	Gallus gallus hephaestin (HEPH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07206	XM_015285254.1 Latest version!	Gallus gallus hephaestin (HEPH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07206	XM_420165.6 Latest version!	Gallus gallus hephaestin (HEPH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OGa07206	XM_004940526.2	Gallus gallus hephaestin (HEPH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa07206	XM_420165.5	Gallus gallus hephaestin (HEPH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEPH ( XM_025150285.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006053.1 Gallus gallus hephaestin (HEPH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa150555
Clone ID Related Accession (Same CDS sequence)	XM_025150285.1
Accession Version	XM_025150285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	hephaestin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCCC AGGATCAGAA GAAGGATGAT GCTGTCTTCC CAGGAGGGAG TTACACCTAC 
ACTTGGACTG TCCCTGAAGA TCACAGCCCG ACAGCTGATG ATCCGAACTG CTTGACCTGG 
ATTTATCACT CTCACATTGA TGCACCAAAG GATATTGCAT CTGGGTTAAT TGGGCCCTTG 
GTAACGTGCA AAGAAGGAAT ACTGACAGGC ACTTCTCAAA GGCGCCAGGA TGTGGATGTT 
GATTTCTTCC TGATGTTCAG TGTGGTAGAT GAGAACTTAA GCTGGTACTT GGATGAGAAC 
ATTGCATCAT TCTGCACAGA TCCGGGATCT GTAGACAAAG AAGATGAGGA ATTCCAAGAG 
AGCAACAAAA TGCATGCTAT CAATGGTTTT GTGTTTGGGA ATCTCCCTGC GCTGACAATG 
TGTGCTGGGG ATCATGTTGC GTGGCACCTC TTTGGCATGG GCAATGAAAT TGACATTCAT 
ACAGCCTACT TCCATGGAGA AACACTCAGC ATTCGAGGCC ATAGGACAGA TGTGGCCAGC 
CTCTTCCCAG CCACTTTTGT TACAGCAGAC ATGATCCCTG GTAACCCTGG GAGATGGCTA 
CTGAGCTGTC AGCTCAATGA TCACATACAA GCTGGGATGG CTGCCATCTA TGAAGTCCGT 
CCATGTTCTC GGCAAGCTCC GGAGCCCACC CTGCAGGGCA GAATTCGGAA GTACTACATA 
GCTGCAAAGG AGGTGCAGTG GGACTATGGA CCTTCAGGAC GTGACAGCAG CAGTGGGAAA 
CAGCTGAGTG AGGCGGGCAG CCCTGCTGAG CCGTTTTTCA AGCGTAGCCT CTACCGAATT 
GGAGGAGTCT ATTGGAAGGT GAAGTATGTG GAGTATACTG ATGAGAGCTT CCGTGAGGAA 
AAGCAGCATT CTGAAGAAGA GAAACACCTT GGGATACTTG GTCCTGTAAT AAAAGCTGAA 
GTTGGTGATA CCATCCTGGT GACGTTTGCT AACAAAGCCT CCTGGCCATT CAGCATCCAG 
CCTCATGGAG TGTCCTATGG GAAGGCATGG GAAGGGATGC GGTACCATGA TGGTGTGTCC 
CAAAATGGGG TGTCTGTTCC ACCTCTGCAC AACTTTACAT ACACGTGGAC TGTACCGAGT 
CATGCTGGGC CGACATCCAG TGACCCTCCC TGCCTGACCT GGATGTACAG CTCTGCTGTG 
GACCCCATCA GAGACACCAG CTCAGGCTTG GTGGGCCCTC TGGTCATCTG CAAGCCGGGC 
ACTTTGGATG ACAACAACAA ACAGAAAGGT ATCGACAAAG AATTTTACCT TCTCTTCAGT 
GTGTTTGATG AGAACCTCAG CTGGTATTTA AGTGCAAACA TAAAGTACTA TTTGAGGATG 
GAAGAGACTT CTCTGAAAAA GGATGATGGC TTTGAGGAAT CGAACAGGAT GCATGCCATT 
AATGGATTTA TGTTTGGTAA CTTGCCTGGG CTGGATATAT GTGAAGGAGA CAACGTGTCC 
TGGCACCTTC TGGGATTGGG CAGTGAAGCA GACGTACATG GAGCTGTGTT TCAGGGAAAC 
ACTCTGAAGA TGAATGGGAT GCGGAAAGAT TCAGCCAACT TATTCCCCCA TACGTTTGCT 
ACTGCCTTCA TGCAACCTGA CAACAAAGGG ACTTTTGAGA TCTACTGCCA GACGAGCAAT 
CATTACCAGG CTGGAATGAG GGAGCAGTAC TCGGTTTCCA ATTGCAACAG GAGGGCTCTG 
TCTCCTGCTG TTCCATACAC AGCAGTACGC ACATACTACA TAGTGGCAGA GGAGATGGAG 
TGGGACTATG CGCCAGACCG GAGCTGGGAG AGGGAGCGGC ACAACCACTC TGCAGAGAGC 
TATGCCGATA TATTTTTGAG CAATAAGGAT GGACTGATTG GCTCCAGGTA CAAGAAAGCA 
GTTTACAGGG AGTACACAGA TGGGACCTTC CAGACCCCTA AGGCCAGGAT AAATGGTGAC 
GAACACCTGG GGATACTAGG TCCTTTTCTG TGGGCAGAAG TGGGAGAGAT TCTCAATGTT 
GTATTCAAGA ACAATGCTTC ACGACCCTAC TCCATTCATG CCCACGGGGT GCTGGAAAGG 
CAGACAGGAC AGCCACAAGT AGCACATCCT GGTGACATAG TGACATACCA ATGGGAAATT 
CCTGAGAGGT CTGGTCCAGG GCCAGATGAT TCAGCCTGTG TCCCTTGGAT CTACTACTCC 
ATGGTAGACC CAGTGAAGGA TATGTACAGT GGTCTGATCG GACCCCTGAA GGTCTGCAGG 
AAAGGGGCAC TGAGAGCTGA TGGCATCAGG AAGGATGTGA AGAGGGAGTT TGCTCTCCTG 
TTCCTGGTTT TTGATGAAAA TCAGTCCTGG TACTTAGAGG AGAATGTGAA GCGTTACAGC 
AGTGGAAGTC ACAAGGATAT TGACCTGCTG GATGAGAAGT TTGTGGAAAG CAACAAAATG 
CACGCTATCA ATGGGAGGCT CTATGCAAAC TTGCCTGGGC TGACCATGTT TGAAGGAGAG 
TGGGTGAACT GGTACCTGCT GGGAATGGGG CAGGAGATTG ATGTCCACAC AGTCCATTTT 
CATGCCGAGA CCTTCATATA CAAGAGTGGC AAAAGCTATA GAGCAGATGT TGTGGATCTC 
TTTCCTGGGA CCTTTGAAAT GGTGGAGATG CTGGCTGGGA ACCCTGGGAC GTGGCTGCTT 
CACTGCCATG TGTCTGATCA TATTCATGCT GGCATGGAGA TACTCTTCCA AGTCTTGCCC 
AGACAAGAGC CAGTGCCTGC AGTTCTGAAC TACAATGAAG AGGCACAACC TGAGGATGAG 
GATGACTCCC AGAAGGTCAG GCTTTTTGGA GCTAAGTTGC CTCTAGGGCA GGTAGAAGCT 
GCAGTCATTA CTCTAGCTGT TATAGGATTG GTCCTCCTCC TGATTGCTGC CTTCCTCCTG 
GGAGTCGTCA TCTACCTCCG GAGGCAAAGG AAGCTGAGAC TCAACCGGAG GTCTATTCTG 
GATGACGGAT TCAAACTCAT GTCTAAAAAG AATTCAGGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006053.1
CDS	623..3667
Translation	MSPQDQKKDDAVFPGGSYTYTWTVPEDHSPTADDPNCLTWIYHSHIDAPKDIASGLIGPLVTCKEGILTGTSQRRQDVDVDFFLMFSVVDENLSWYLDENIASFCTDPGSVDKEDEEFQESNKMHAINGFVFGNLPALTMCAGDHVAWHLFGMGNEIDIHTAYFHGETLSIRGHRTDVASLFPATFVTADMIPGNPGRWLLSCQLNDHIQAGMAAIYEVRPCSRQAPEPTLQGRIRKYYIAAKEVQWDYGPSGRDSSSGKQLSEAGSPAEPFFKRSLYRIGGVYWKVKYVEYTDESFREEKQHSEEEKHLGILGPVIKAEVGDTILVTFANKASWPFSIQPHGVSYGKAWEGMRYHDGVSQNGVSVPPLHNFTYTWTVPSHAGPTSSDPPCLTWMYSSAVDPIRDTSSGLVGPLVICKPGTLDDNNKQKGIDKEFYLLFSVFDENLSWYLSANIKYYLRMEETSLKKDDGFEESNRMHAINGFMFGNLPGLDICEGDNVSWHLLGLGSEADVHGAVFQGNTLKMNGMRKDSANLFPHTFATAFMQPDNKGTFEIYCQTSNHYQAGMREQYSVSNCNRRALSPAVPYTAVRTYYIVAEEMEWDYAPDRSWERERHNHSAESYADIFLSNKDGLIGSRYKKAVYREYTDGTFQTPKARINGDEHLGILGPFLWAEVGEILNVVFKNNASRPYSIHAHGVLERQTGQPQVAHPGDIVTYQWEIPERSGPGPDDSACVPWIYYSMVDPVKDMYSGLIGPLKVCRKGALRADGIRKDVKREFALLFLVFDENQSWYLEENVKRYSSGSHKDIDLLDEKFVESNKMHAINGRLYANLPGLTMFEGEWVNWYLLGMGQEIDVHTVHFHAETFIYKSGKSYRADVVDLFPGTFEMVEMLAGNPGTWLLHCHVSDHIHAGMEILFQVLPRQEPVPAVLNYNEEAQPEDEDDSQKVRLFGAKLPLGQVEAAVITLAVIGLVLLLIAAFLLGVVIYLRRQRKLRLNRRSILDDGFKLMSKKNSGL

Target ORF information:

RefSeq Version	XM_025150285.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hephaestin (HEPH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150285.1

ORF Insert Sequence:

ATGTCCCCCC AGGATCAGAA GAAGGATGAT GCTGTCTTCC CAGGAGGGAG TTACACCTAC 
ACTTGGACTG TCCCTGAAGA TCACAGCCCG ACAGCTGATG ATCCGAACTG CTTGACCTGG 
ATTTATCACT CTCACATTGA TGCACCAAAG GATATTGCAT CTGGGTTAAT TGGGCCCTTG 
GTAACGTGCA AAGAAGGAAT ACTGACAGGC ACTTCTCAAA GGCGCCAGGA TGTGGATGTT 
GATTTCTTCC TGATGTTCAG TGTGGTAGAT GAGAACTTAA GCTGGTACTT GGATGAGAAC 
ATTGCATCAT TCTGCACAGA TCCGGGATCT GTAGACAAAG AAGATGAGGA ATTCCAAGAG 
AGCAACAAAA TGCATGCTAT CAATGGTTTT GTGTTTGGGA ATCTCCCTGC GCTGACAATG 
TGTGCTGGGG ATCATGTTGC GTGGCACCTC TTTGGCATGG GCAATGAAAT TGACATTCAT 
ACAGCCTACT TCCATGGAGA AACACTCAGC ATTCGAGGCC ATAGGACAGA TGTGGCCAGC 
CTCTTCCCAG CCACTTTTGT TACAGCAGAC ATGATCCCTG GTAACCCTGG GAGATGGCTA 
CTGAGCTGTC AGCTCAATGA TCACATACAA GCTGGGATGG CTGCCATCTA TGAAGTCCGT 
CCATGTTCTC GGCAAGCTCC GGAGCCCACC CTGCAGGGCA GAATTCGGAA GTACTACATA 
GCTGCAAAGG AGGTGCAGTG GGACTATGGA CCTTCAGGAC GTGACAGCAG CAGTGGGAAA 
CAGCTGAGTG AGGCGGGCAG CCCTGCTGAG CCGTTTTTCA AGCGTAGCCT CTACCGAATT 
GGAGGAGTCT ATTGGAAGGT GAAGTATGTG GAGTATACTG ATGAGAGCTT CCGTGAGGAA 
AAGCAGCATT CTGAAGAAGA GAAACACCTT GGGATACTTG GTCCTGTAAT AAAAGCTGAA 
GTTGGTGATA CCATCCTGGT GACGTTTGCT AACAAAGCCT CCTGGCCATT CAGCATCCAG 
CCTCATGGAG TGTCCTATGG GAAGGCATGG GAAGGGATGC GGTACCATGA TGGTGTGTCC 
CAAAATGGGG TGTCTGTTCC ACCTCTGCAC AACTTTACAT ACACGTGGAC TGTACCGAGT 
CATGCTGGGC CGACATCCAG TGACCCTCCC TGCCTGACCT GGATGTACAG CTCTGCTGTG 
GACCCCATCA GAGACACCAG CTCAGGCTTG GTGGGCCCTC TGGTCATCTG CAAGCCGGGC 
ACTTTGGATG ACAACAACAA ACAGAAAGGT ATCGACAAAG AATTTTACCT TCTCTTCAGT 
GTGTTTGATG AGAACCTCAG CTGGTATTTA AGTGCAAACA TAAAGTACTA TTTGAGGATG 
GAAGAGACTT CTCTGAAAAA GGATGATGGC TTTGAGGAAT CGAACAGGAT GCATGCCATT 
AATGGATTTA TGTTTGGTAA CTTGCCTGGG CTGGATATAT GTGAAGGAGA CAACGTGTCC 
TGGCACCTTC TGGGATTGGG CAGTGAAGCA GACGTACATG GAGCTGTGTT TCAGGGAAAC 
ACTCTGAAGA TGAATGGGAT GCGGAAAGAT TCAGCCAACT TATTCCCCCA TACGTTTGCT 
ACTGCCTTCA TGCAACCTGA CAACAAAGGG ACTTTTGAGA TCTACTGCCA GACGAGCAAT 
CATTACCAGG CTGGAATGAG GGAGCAGTAC TCGGTTTCCA ATTGCAACAG GAGGGCTCTG 
TCTCCTGCTG TTCCATACAC AGCAGTACGC ACATACTACA TAGTGGCAGA GGAGATGGAG 
TGGGACTATG CGCCAGACCG GAGCTGGGAG AGGGAGCGGC ACAACCACTC TGCAGAGAGC 
TATGCCGATA TATTTTTGAG CAATAAGGAT GGACTGATTG GCTCCAGGTA CAAGAAAGCA 
GTTTACAGGG AGTACACAGA TGGGACCTTC CAGACCCCTA AGGCCAGGAT AAATGGTGAC 
GAACACCTGG GGATACTAGG TCCTTTTCTG TGGGCAGAAG TGGGAGAGAT TCTCAATGTT 
GTATTCAAGA ACAATGCTTC ACGACCCTAC TCCATTCATG CCCACGGGGT GCTGGAAAGG 
CAGACAGGAC AGCCACAAGT AGCACATCCT GGTGACATAG TGACATACCA ATGGGAAATT 
CCTGAGAGGT CTGGTCCAGG GCCAGATGAT TCAGCCTGTG TCCCTTGGAT CTACTACTCC 
ATGGTAGACC CAGTGAAGGA TATGTACAGT GGTCTGATCG GACCCCTGAA GGTCTGCAGG 
AAAGGGGCAC TGAGAGCTGA TGGCATCAGG AAGGATGTGA AGAGGGAGTT TGCTCTCCTG 
TTCCTGGTTT TTGATGAAAA TCAGTCCTGG TACTTAGAGG AGAATGTGAA GCGTTACAGC 
AGTGGAAGTC ACAAGGATAT TGACCTGCTG GATGAGAAGT TTGTGGAAAG CAACAAAATG 
CACGCTATCA ATGGGAGGCT CTATGCAAAC TTGCCTGGGC TGACCATGTT TGAAGGAGAG 
TGGGTGAACT GGTACCTGCT GGGAATGGGG CAGGAGATTG ATGTCCACAC AGTCCATTTT 
CATGCCGAGA CCTTCATATA CAAGAGTGGC AAAAGCTATA GAGCAGATGT TGTGGATCTC 
TTTCCTGGGA CCTTTGAAAT GGTGGAGATG CTGGCTGGGA ACCCTGGGAC GTGGCTGCTT 
CACTGCCATG TGTCTGATCA TATTCATGCT GGCATGGAGA TACTCTTCCA AGTCTTGCCC 
AGACAAGAGC CAGTGCCTGC AGTTCTGAAC TACAATGAAG AGGCACAACC TGAGGATGAG 
GATGACTCCC AGAAGGTCAG GCTTTTTGGA GCTAAGTTGC CTCTAGGGCA GGTAGAAGCT 
GCAGTCATTA CTCTAGCTGT TATAGGATTG GTCCTCCTCC TGATTGCTGC CTTCCTCCTG 
GGAGTCGTCA TCTACCTCCG GAGGCAAAGG AAGCTGAGAC TCAACCGGAG GTCTATTCTG 
GATGACGGAT TCAAACTCAT GTCTAAAAAG AATTCAGGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_420165.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420165.4 Gallus gallus hephaestin (HEPH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa07206
Clone ID Related Accession (Same CDS sequence)	XM_420165.6 , XM_004940526.3 , XM_004940526.2 , XM_015285254.1 , XM_420165.5
Accession Version	XM_420165.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420165.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420165.4
CDS	150..3653
Translation	MYPPEEHSAHTTMRLFWLLLLCVHWLSPVFAGGITRVYYLGIREVDWNYAPTGMNVISNQSITRSLKASVFLQPGKDRIGGTYKKSVYKQYTDSTYTTEIPKPAWLGFLGPIIRAEVGDTIKVHLKNFASRPYTIHPHGVFYEKGSEGSLYPDMSPQDQKKDDAVFPGGSYTYTWTVPEDHSPTADDPNCLTWIYHSHIDAPKDIASGLIGPLVTCKEGILTGTSQRRQDVDVDFFLMFSVVDENLSWYLDENIASFCTDPGSVDKEDEEFQESNKMHAINGFVFGNLPALTMCAGDHVAWHLFGMGNEIDIHTAYFHGETLSIRGHRTDVASLFPATFVTADMIPGNPGRWLLSCQLNDHIQAGMAAIYEVRPCSRQAPEPTLQGRIRKYYIAAKEVQWDYGPSGRDSSSGKQLSEAGSPAEPFFKRSLYRIGGVYWKVKYVEYTDESFREEKQHSEEEKHLGILGPVIKAEVGDTILVTFANKASWPFSIQPHGVSYGKAWEGMRYHDGVSQNGVSVPPLHNFTYTWTVPSHAGPTSSDPPCLTWMYSSAVDPIRDTSSGLVGPLVICKPGTLDDNNKQKGIDKEFYLLFSVFDENLSWYLSANIKYYLRMEETSLKKDDGFEESNRMHAINGFMFGNLPGLDICEGDNVSWHLLGLGSEADVHGAVFQGNTLKMNGMRKDSANLFPHTFATAFMQPDNKGTFEIYCQTSNHYQAGMREQYSVSNCNRRALSPAVPYTAVRTYYIVAEEMEWDYAPDRSWERERHNHSAESYADIFLSNKDGLIGSRYKKAVYREYTDGTFQTPKARINGDEHLGILGPFLWAEVGEILNVVFKNNASRPYSIHAHGVLERQTGQPQVAHPGDIVTYQWEIPERSGPGPDDSACVPWIYYSMVDPVKDMYSGLIGPLKVCRKGALRADGIRKDVKREFALLFLVFDENQSWYLEENVKRYSSGSHKDIDLLDEKFVESNKMHAINGRLYANLPGLTMFEGEWVNWYLLGMGQEIDVHTVHFHAETFIYKSGKSYRADVVDLFPGTFEMVEMLAGNPGTWLLHCHVSDHIHAGMEILFQVLPRQEPVPAVLNYNEEAQPEDEDDSQKVRLFGAKLPLGQVEAAVITLAVIGLVLLLIAAFLLGVVIYLRRQRKLRLNRRSILDDGFKLMSKKNSGL

Target ORF information:

RefSeq Version	XM_420165.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hephaestin (HEPH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420165.6

ORF Insert Sequence:

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_004940526.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940583.1 Gallus gallus hephaestin (HEPH), transcript variant X2, mRNA.

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CloneID	OGa07206
Clone ID Related Accession (Same CDS sequence)	XM_420165.6 , XM_004940526.3 , XM_004940526.2 , XM_015285254.1 , XM_420165.5
Accession Version	XM_004940526.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940526.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940583.1
CDS	334..3837
Translation	MYPPEEHSAHTTMRLFWLLLLCVHWLSPVFAGGITRVYYLGIREVDWNYAPTGMNVISNQSITRSLKASVFLQPGKDRIGGTYKKSVYKQYTDSTYTTEIPKPAWLGFLGPIIRAEVGDTIKVHLKNFASRPYTIHPHGVFYEKGSEGSLYPDMSPQDQKKDDAVFPGGSYTYTWTVPEDHSPTADDPNCLTWIYHSHIDAPKDIASGLIGPLVTCKEGILTGTSQRRQDVDVDFFLMFSVVDENLSWYLDENIASFCTDPGSVDKEDEEFQESNKMHAINGFVFGNLPALTMCAGDHVAWHLFGMGNEIDIHTAYFHGETLSIRGHRTDVASLFPATFVTADMIPGNPGRWLLSCQLNDHIQAGMAAIYEVRPCSRQAPEPTLQGRIRKYYIAAKEVQWDYGPSGRDSSSGKQLSEAGSPAEPFFKRSLYRIGGVYWKVKYVEYTDESFREEKQHSEEEKHLGILGPVIKAEVGDTILVTFANKASWPFSIQPHGVSYGKAWEGMRYHDGVSQNGVSVPPLHNFTYTWTVPSHAGPTSSDPPCLTWMYSSAVDPIRDTSSGLVGPLVICKPGTLDDNNKQKGIDKEFYLLFSVFDENLSWYLSANIKYYLRMEETSLKKDDGFEESNRMHAINGFMFGNLPGLDICEGDNVSWHLLGLGSEADVHGAVFQGNTLKMNGMRKDSANLFPHTFATAFMQPDNKGTFEIYCQTSNHYQAGMREQYSVSNCNRRALSPAVPYTAVRTYYIVAEEMEWDYAPDRSWERERHNHSAESYADIFLSNKDGLIGSRYKKAVYREYTDGTFQTPKARINGDEHLGILGPFLWAEVGEILNVVFKNNASRPYSIHAHGVLERQTGQPQVAHPGDIVTYQWEIPERSGPGPDDSACVPWIYYSMVDPVKDMYSGLIGPLKVCRKGALRADGIRKDVKREFALLFLVFDENQSWYLEENVKRYSSGSHKDIDLLDEKFVESNKMHAINGRLYANLPGLTMFEGEWVNWYLLGMGQEIDVHTVHFHAETFIYKSGKSYRADVVDLFPGTFEMVEMLAGNPGTWLLHCHVSDHIHAGMEILFQVLPRQEPVPAVLNYNEEAQPEDEDDSQKVRLFGAKLPLGQVEAAVITLAVIGLVLLLIAAFLLGVVIYLRRQRKLRLNRRSILDDGFKLMSKKNSGL

Target ORF information:

RefSeq Version	XM_004940526.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hephaestin (HEPH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940526.3

ORF Insert Sequence:

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_004940526.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940583.1 Gallus gallus hephaestin (HEPH), transcript variant X2, mRNA.

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CloneID	OGa07206
Clone ID Related Accession (Same CDS sequence)	XM_420165.6 , XM_004940526.3 , XM_004940526.2 , XM_015285254.1 , XM_420165.5
Accession Version	XM_004940526.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	hephaestin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940583.1
CDS	345..3848
Translation	MYPPEEHSAHTTMRLFWLLLLCVHWLSPVFAGGITRVYYLGIREVDWNYAPTGMNVISNQSITRSLKASVFLQPGKDRIGGTYKKSVYKQYTDSTYTTEIPKPAWLGFLGPIIRAEVGDTIKVHLKNFASRPYTIHPHGVFYEKGSEGSLYPDMSPQDQKKDDAVFPGGSYTYTWTVPEDHSPTADDPNCLTWIYHSHIDAPKDIASGLIGPLVTCKEGILTGTSQRRQDVDVDFFLMFSVVDENLSWYLDENIASFCTDPGSVDKEDEEFQESNKMHAINGFVFGNLPALTMCAGDHVAWHLFGMGNEIDIHTAYFHGETLSIRGHRTDVASLFPATFVTADMIPGNPGRWLLSCQLNDHIQAGMAAIYEVRPCSRQAPEPTLQGRIRKYYIAAKEVQWDYGPSGRDSSSGKQLSEAGSPAEPFFKRSLYRIGGVYWKVKYVEYTDESFREEKQHSEEEKHLGILGPVIKAEVGDTILVTFANKASWPFSIQPHGVSYGKAWEGMRYHDGVSQNGVSVPPLHNFTYTWTVPSHAGPTSSDPPCLTWMYSSAVDPIRDTSSGLVGPLVICKPGTLDDNNKQKGIDKEFYLLFSVFDENLSWYLSANIKYYLRMEETSLKKDDGFEESNRMHAINGFMFGNLPGLDICEGDNVSWHLLGLGSEADVHGAVFQGNTLKMNGMRKDSANLFPHTFATAFMQPDNKGTFEIYCQTSNHYQAGMREQYSVSNCNRRALSPAVPYTAVRTYYIVAEEMEWDYAPDRSWERERHNHSAESYADIFLSNKDGLIGSRYKKAVYREYTDGTFQTPKARINGDEHLGILGPFLWAEVGEILNVVFKNNASRPYSIHAHGVLERQTGQPQVAHPGDIVTYQWEIPERSGPGPDDSACVPWIYYSMVDPVKDMYSGLIGPLKVCRKGALRADGIRKDVKREFALLFLVFDENQSWYLEENVKRYSSGSHKDIDLLDEKFVESNKMHAINGRLYANLPGLTMFEGEWVNWYLLGMGQEIDVHTVHFHAETFIYKSGKSYRADVVDLFPGTFEMVEMLAGNPGTWLLHCHVSDHIHAGMEILFQVLPRQEPVPAVLNYNEEAQPEDEDDSQKVRLFGAKLPLGQVEAAVITLAVIGLVLLLIAAFLLGVVIYLRRQRKLRLNRRSILDDGFKLMSKKNSGL

Target ORF information:

RefSeq Version	XM_004940526.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hephaestin (HEPH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940526.2

ORF Insert Sequence:

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_015285254.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140740.1 Gallus gallus hephaestin (HEPH), transcript variant X3, mRNA.

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CloneID	OGa07206
Clone ID Related Accession (Same CDS sequence)	XM_420165.6 , XM_004940526.3 , XM_004940526.2 , XM_015285254.1 , XM_420165.5
Accession Version	XM_015285254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	hephaestin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140740.1
CDS	102..3605
Translation	MYPPEEHSAHTTMRLFWLLLLCVHWLSPVFAGGITRVYYLGIREVDWNYAPTGMNVISNQSITRSLKASVFLQPGKDRIGGTYKKSVYKQYTDSTYTTEIPKPAWLGFLGPIIRAEVGDTIKVHLKNFASRPYTIHPHGVFYEKGSEGSLYPDMSPQDQKKDDAVFPGGSYTYTWTVPEDHSPTADDPNCLTWIYHSHIDAPKDIASGLIGPLVTCKEGILTGTSQRRQDVDVDFFLMFSVVDENLSWYLDENIASFCTDPGSVDKEDEEFQESNKMHAINGFVFGNLPALTMCAGDHVAWHLFGMGNEIDIHTAYFHGETLSIRGHRTDVASLFPATFVTADMIPGNPGRWLLSCQLNDHIQAGMAAIYEVRPCSRQAPEPTLQGRIRKYYIAAKEVQWDYGPSGRDSSSGKQLSEAGSPAEPFFKRSLYRIGGVYWKVKYVEYTDESFREEKQHSEEEKHLGILGPVIKAEVGDTILVTFANKASWPFSIQPHGVSYGKAWEGMRYHDGVSQNGVSVPPLHNFTYTWTVPSHAGPTSSDPPCLTWMYSSAVDPIRDTSSGLVGPLVICKPGTLDDNNKQKGIDKEFYLLFSVFDENLSWYLSANIKYYLRMEETSLKKDDGFEESNRMHAINGFMFGNLPGLDICEGDNVSWHLLGLGSEADVHGAVFQGNTLKMNGMRKDSANLFPHTFATAFMQPDNKGTFEIYCQTSNHYQAGMREQYSVSNCNRRALSPAVPYTAVRTYYIVAEEMEWDYAPDRSWERERHNHSAESYADIFLSNKDGLIGSRYKKAVYREYTDGTFQTPKARINGDEHLGILGPFLWAEVGEILNVVFKNNASRPYSIHAHGVLERQTGQPQVAHPGDIVTYQWEIPERSGPGPDDSACVPWIYYSMVDPVKDMYSGLIGPLKVCRKGALRADGIRKDVKREFALLFLVFDENQSWYLEENVKRYSSGSHKDIDLLDEKFVESNKMHAINGRLYANLPGLTMFEGEWVNWYLLGMGQEIDVHTVHFHAETFIYKSGKSYRADVVDLFPGTFEMVEMLAGNPGTWLLHCHVSDHIHAGMEILFQVLPRQEPVPAVLNYNEEAQPEDEDDSQKVRLFGAKLPLGQVEAAVITLAVIGLVLLLIAAFLLGVVIYLRRQRKLRLNRRSILDDGFKLMSKKNSGL

Target ORF information:

RefSeq Version	XM_015285254.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hephaestin (HEPH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285254.1

ORF Insert Sequence:

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_420165.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420165.4 Gallus gallus hephaestin (HEPH), transcript variant X1, mRNA.

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CloneID	OGa07206
Clone ID Related Accession (Same CDS sequence)	XM_420165.6 , XM_004940526.3 , XM_004940526.2 , XM_015285254.1 , XM_420165.5
Accession Version	XM_420165.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	hephaestin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420165.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420165.4
CDS	119..3622
Translation	MYPPEEHSAHTTMRLFWLLLLCVHWLSPVFAGGITRVYYLGIREVDWNYAPTGMNVISNQSITRSLKASVFLQPGKDRIGGTYKKSVYKQYTDSTYTTEIPKPAWLGFLGPIIRAEVGDTIKVHLKNFASRPYTIHPHGVFYEKGSEGSLYPDMSPQDQKKDDAVFPGGSYTYTWTVPEDHSPTADDPNCLTWIYHSHIDAPKDIASGLIGPLVTCKEGILTGTSQRRQDVDVDFFLMFSVVDENLSWYLDENIASFCTDPGSVDKEDEEFQESNKMHAINGFVFGNLPALTMCAGDHVAWHLFGMGNEIDIHTAYFHGETLSIRGHRTDVASLFPATFVTADMIPGNPGRWLLSCQLNDHIQAGMAAIYEVRPCSRQAPEPTLQGRIRKYYIAAKEVQWDYGPSGRDSSSGKQLSEAGSPAEPFFKRSLYRIGGVYWKVKYVEYTDESFREEKQHSEEEKHLGILGPVIKAEVGDTILVTFANKASWPFSIQPHGVSYGKAWEGMRYHDGVSQNGVSVPPLHNFTYTWTVPSHAGPTSSDPPCLTWMYSSAVDPIRDTSSGLVGPLVICKPGTLDDNNKQKGIDKEFYLLFSVFDENLSWYLSANIKYYLRMEETSLKKDDGFEESNRMHAINGFMFGNLPGLDICEGDNVSWHLLGLGSEADVHGAVFQGNTLKMNGMRKDSANLFPHTFATAFMQPDNKGTFEIYCQTSNHYQAGMREQYSVSNCNRRALSPAVPYTAVRTYYIVAEEMEWDYAPDRSWERERHNHSAESYADIFLSNKDGLIGSRYKKAVYREYTDGTFQTPKARINGDEHLGILGPFLWAEVGEILNVVFKNNASRPYSIHAHGVLERQTGQPQVAHPGDIVTYQWEIPERSGPGPDDSACVPWIYYSMVDPVKDMYSGLIGPLKVCRKGALRADGIRKDVKREFALLFLVFDENQSWYLEENVKRYSSGSHKDIDLLDEKFVESNKMHAINGRLYANLPGLTMFEGEWVNWYLLGMGQEIDVHTVHFHAETFIYKSGKSYRADVVDLFPGTFEMVEMLAGNPGTWLLHCHVSDHIHAGMEILFQVLPRQEPVPAVLNYNEEAQPEDEDDSQKVRLFGAKLPLGQVEAAVITLAVIGLVLLLIAAFLLGVVIYLRRQRKLRLNRRSILDDGFKLMSKKNSGL

Target ORF information:

RefSeq Version	XM_420165.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hephaestin (HEPH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420165.5

ORF Insert Sequence:

ATGTACCCTC CAGAAGAGCA TTCTGCTCAT ACCACAATGC GGTTGTTCTG GCTGTTGCTG 
CTTTGTGTCC ATTGGCTGTC GCCTGTGTTC GCTGGAGGCA TCACCCGAGT CTATTACCTG 
GGGATCCGTG AGGTGGACTG GAATTATGCT CCAACAGGAA TGAATGTGAT CAGCAACCAA 
AGCATCACAC GTAGCCTAAA GGCCTCAGTG TTCCTCCAGC CTGGCAAGGA CAGGATAGGA 
GGTACATACA AGAAATCTGT CTATAAGCAA TACACAGACT CCACCTACAC CACTGAGATT 
CCCAAACCGG CGTGGCTGGG GTTCCTTGGG CCCATCATCC GAGCAGAAGT GGGAGATACC 
ATTAAAGTAC ACCTGAAAAA CTTTGCCAGT CGGCCATATA CAATCCATCC TCACGGTGTC 
TTCTACGAGA AGGGCTCTGA AGGATCCCTG TATCCCGATA TGTCCCCCCA GGATCAGAAG 
AAGGATGATG CTGTCTTCCC AGGAGGGAGT TACACCTACA CTTGGACTGT CCCTGAAGAT 
CACAGCCCGA CAGCTGATGA TCCGAACTGC TTGACCTGGA TTTATCACTC TCACATTGAT 
GCACCAAAGG ATATTGCATC TGGGTTAATT GGGCCCTTGG TAACGTGCAA AGAAGGAATA 
CTGACAGGCA CTTCTCAAAG GCGCCAGGAT GTGGATGTTG ATTTCTTCCT GATGTTCAGT 
GTGGTAGATG AGAACTTAAG CTGGTACTTG GATGAGAACA TTGCATCATT CTGCACAGAT 
CCGGGATCTG TAGACAAAGA AGATGAGGAA TTCCAAGAGA GCAACAAAAT GCATGCTATC 
AATGGTTTTG TGTTTGGGAA TCTCCCTGCG CTGACAATGT GTGCTGGGGA TCATGTTGCG 
TGGCACCTCT TTGGCATGGG CAATGAAATT GACATTCATA CAGCCTACTT CCATGGAGAA 
ACACTCAGCA TTCGAGGCCA TAGGACAGAT GTGGCCAGCC TCTTCCCAGC CACTTTTGTT 
ACAGCAGACA TGATCCCTGG TAACCCTGGG AGATGGCTAC TGAGCTGTCA GCTCAATGAT 
CACATACAAG CTGGGATGGC TGCCATCTAT GAAGTCCGTC CATGTTCTCG GCAAGCTCCG 
GAGCCCACCC TGCAGGGCAG AATTCGGAAG TACTACATAG CTGCAAAGGA GGTGCAGTGG 
GACTATGGAC CTTCAGGACG TGACAGCAGC AGTGGGAAAC AGCTGAGTGA GGCGGGCAGC 
CCTGCTGAGC CGTTTTTCAA GCGTAGCCTC TACCGAATTG GAGGAGTCTA TTGGAAGGTG 
AAGTATGTGG AGTATACTGA TGAGAGCTTC CGTGAGGAAA AGCAGCATTC TGAAGAAGAG 
AAACACCTTG GGATACTTGG TCCTGTAATA AAAGCTGAAG TTGGTGATAC CATCCTGGTG 
ACGTTTGCTA ACAAAGCCTC CTGGCCATTC AGCATCCAGC CTCATGGAGT GTCCTATGGG 
AAGGCATGGG AAGGGATGCG GTACCATGAT GGTGTGTCCC AAAATGGGGT GTCTGTTCCA 
CCTCTGCACA ACTTTACATA CACGTGGACT GTACCGAGTC ATGCTGGGCC GACATCCAGT 
GACCCTCCCT GCCTGACCTG GATGTACAGC TCTGCTGTGG ACCCCATCAG AGACACCAGC 
TCAGGCTTGG TGGGCCCTCT GGTCATCTGC AAGCCGGGCA CTTTGGATGA CAACAACAAA 
CAGAAAGGTA TCGACAAAGA ATTTTACCTT CTCTTCAGTG TGTTTGATGA GAACCTCAGC 
TGGTATTTAA GTGCAAACAT AAAGTACTAT TTGAGGATGG AAGAGACTTC TCTGAAAAAG 
GATGATGGCT TTGAGGAATC GAACAGGATG CATGCCATTA ATGGATTTAT GTTTGGTAAC 
TTGCCTGGGC TGGATATATG TGAAGGAGAC AACGTGTCCT GGCACCTTCT GGGATTGGGC 
AGTGAAGCAG ACGTACATGG AGCTGTGTTT CAGGGAAACA CTCTGAAGAT GAATGGGATG 
CGGAAAGATT CAGCCAACTT ATTCCCCCAT ACGTTTGCTA CTGCCTTCAT GCAACCTGAC 
AACAAAGGGA CTTTTGAGAT CTACTGCCAG ACGAGCAATC ATTACCAGGC TGGAATGAGG 
GAGCAGTACT CGGTTTCCAA TTGCAACAGG AGGGCTCTGT CTCCTGCTGT TCCATACACA 
GCAGTACGCA CATACTACAT AGTGGCAGAG GAGATGGAGT GGGACTATGC GCCAGACCGG 
AGCTGGGAGA GGGAGCGGCA CAACCACTCT GCAGAGAGCT ATGCCGATAT ATTTTTGAGC 
AATAAGGATG GACTGATTGG CTCCAGGTAC AAGAAAGCAG TTTACAGGGA GTACACAGAT 
GGGACCTTCC AGACCCCTAA GGCCAGGATA AATGGTGACG AACACCTGGG GATACTAGGT 
CCTTTTCTGT GGGCAGAAGT GGGAGAGATT CTCAATGTTG TATTCAAGAA CAATGCTTCA 
CGACCCTACT CCATTCATGC CCACGGGGTG CTGGAAAGGC AGACAGGACA GCCACAAGTA 
GCACATCCTG GTGACATAGT GACATACCAA TGGGAAATTC CTGAGAGGTC TGGTCCAGGG 
CCAGATGATT CAGCCTGTGT CCCTTGGATC TACTACTCCA TGGTAGACCC AGTGAAGGAT 
ATGTACAGTG GTCTGATCGG ACCCCTGAAG GTCTGCAGGA AAGGGGCACT GAGAGCTGAT 
GGCATCAGGA AGGATGTGAA GAGGGAGTTT GCTCTCCTGT TCCTGGTTTT TGATGAAAAT 
CAGTCCTGGT ACTTAGAGGA GAATGTGAAG CGTTACAGCA GTGGAAGTCA CAAGGATATT 
GACCTGCTGG ATGAGAAGTT TGTGGAAAGC AACAAAATGC ACGCTATCAA TGGGAGGCTC 
TATGCAAACT TGCCTGGGCT GACCATGTTT GAAGGAGAGT GGGTGAACTG GTACCTGCTG 
GGAATGGGGC AGGAGATTGA TGTCCACACA GTCCATTTTC ATGCCGAGAC CTTCATATAC 
AAGAGTGGCA AAAGCTATAG AGCAGATGTT GTGGATCTCT TTCCTGGGAC CTTTGAAATG 
GTGGAGATGC TGGCTGGGAA CCCTGGGACG TGGCTGCTTC ACTGCCATGT GTCTGATCAT 
ATTCATGCTG GCATGGAGAT ACTCTTCCAA GTCTTGCCCA GACAAGAGCC AGTGCCTGCA 
GTTCTGAACT ACAATGAAGA GGCACAACCT GAGGATGAGG ATGACTCCCA GAAGGTCAGG 
CTTTTTGGAG CTAAGTTGCC TCTAGGGCAG GTAGAAGCTG CAGTCATTAC TCTAGCTGTT 
ATAGGATTGG TCCTCCTCCT GATTGCTGCC TTCCTCCTGG GAGTCGTCAT CTACCTCCGG 
AGGCAAAGGA AGCTGAGACT CAACCGGAGG TCTATTCTGG ATGACGGATT CAAACTCATG 
TCTAAAAAGA ATTCAGGGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HEPH cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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