HEPH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEPH
Entrez Gene ID	9843
Full Name	hephaestin
Synonyms	CPL
General protein information	Preferred Names hephaestin Names hephaestin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq12
Summary	This gene encodes a member of the multicopper oxidase protein family. The encoded protein is involved in the transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system, and may be involved in copper transport and homeostasis. In mouse, defects in this gene can lead to severe microcytic anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013].
Disorder MIM:	300167

mRNA and Protein(s)

mRNA	Protein	Name
XM_017029999.1	XP_016885488.1	hephaestin isoform X6
XM_017030001.1	XP_016885490.1	hephaestin isoform X8
XM_017030003.1	XP_016885492.1	hephaestin isoform X10
NM_001367238.1	NP_001354167.1	hephaestin isoform g precursor
NM_014799.3	NP_055614.1	hephaestin isoform b
NM_138737.4	NP_620074.3	hephaestin isoform a
NM_001282141.1	NP_001269070.1	hephaestin isoform d precursor
NM_001367236.1	NP_001354165.1	hephaestin isoform f precursor
NM_001367242.2	NP_001354171.1	hephaestin isoform b
NM_001367233.2	NP_001354162.1	hephaestin isoform e precursor
XM_006724722.1	XP_006724785.1	hephaestin isoform X2
XM_017029998.1	XP_016885487.1	hephaestin isoform X5
XM_011531073.2	XP_011529375.1	hephaestin isoform X1
NM_001367234.1	NP_001354163.1	hephaestin isoform e precursor
NM_001367239.1	NP_001354168.1	hephaestin isoform h precursor
NM_001130860.4	NP_001124332.1	hephaestin isoform c precursor
NM_001367236.2	NP_001354165.1	hephaestin isoform f precursor
XM_017029998.2	XP_016885487.1	hephaestin isoform X5
XM_017030002.1	XP_016885491.1	hephaestin isoform X9
NM_001367232.1	NP_001354161.1	hephaestin isoform e precursor
NM_001367233.1	NP_001354162.1	hephaestin isoform e precursor
NM_001367242.1	NP_001354171.1	hephaestin isoform b
NM_001367243.1	NP_001354172.1	hephaestin isoform k precursor
NM_001367243.2	NP_001354172.1	hephaestin isoform k precursor
NM_001367240.2	NP_001354169.1	hephaestin isoform i precursor
NM_001282141.2	NP_001269070.1	hephaestin isoform d precursor
NM_001367238.2	NP_001354167.1	hephaestin isoform g precursor
XM_017030000.1	XP_016885489.1	hephaestin isoform X7
NM_001367232.2	NP_001354161.1	hephaestin isoform e precursor
NM_014799.4	NP_055614.1	hephaestin isoform b
XM_011531073.3	XP_011529375.1	hephaestin isoform X1
NM_138737.5	NP_620074.3	hephaestin isoform a
NM_001130860.3	NP_001124332.1	hephaestin isoform c precursor
NM_001367240.1	NP_001354169.1	hephaestin isoform i precursor
NM_001367241.1	NP_001354170.1	hephaestin isoform j precursor
XM_011531074.2	XP_011529376.1	hephaestin isoform X3
XM_011531075.2	XP_011529377.1	hephaestin isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEPH	NP_620074.3
Pan troglodytes (chimpanzee)	HEPH	XP_003954062.1
	HEPH	XP_001099571.2
Bos taurus (cattle)	HEPH	XP_005228323.1
Canis lupus familiaris (dog)	HEPH	XP_005641482.1
Mus musculus (house mouse)	Heph	NP_001153100.1
Rattus norvegicus (Norway rat)	Heph	NP_579838.1
Gallus gallus (chicken)	HEPH	XP_420165.4

Gene Ontology
Bibliography

Related articles in PubMed

[Ceruloplasmin, hephaestin and zyklopen: the three multicopper oxidases important for human iron metabolism].
Wierzbicka D, Gromadzka G
Postepy higieny i medycyny doswiadczalnej (Online)68912-24(2014)

Ferroportin and exocytoplasmic ferroxidase activity are required for brain microvascular endothelial cell iron efflux.
McCarthy RC, Kosman DJ
The Journal of biological chemistry288(24)17932-40(2013 Jun)

Functional role of the putative iron ligands in the ferroxidase activity of recombinant human hephaestin.
Vashchenko G, Macgillivray RT
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry17(8)1187-95(2012 Dec)

An X chromosome association scan of the Norfolk Island genetic isolate provides evidence for a novel migraine susceptibility locus at Xq12.
Maher BH, Lea RA, Benton M, Cox HC, Bellis C, Carless M, Dyer TD, Curran J, Charlesworth JC, Buring JE, Kurth T, Chasman DI, Ridker PM, Sch?rks M, Blangero J, Griffiths LR
PloS one7(5)e37903(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This review describes function of hephaestin as ferroxidase is essential for iron binding to apotransferrin in the lamina propria of the intestinal mucosa, a process that is important for further transport of iron to the liver by the portal vein.
Title: [Ceruloplasmin, hephaestin and zyklopen: the three multicopper oxidases important for human iron metabolism].

Iron efflux from human brain microvasculature endothelial cells ferroportin requires the action of an exocytoplasmic ferroxidase which can be either endogenous hephaestin or extracellular ceruloplasmin.
Title: Ferroportin and exocytoplasmic ferroxidase activity are required for brain microvascular endothelial cell iron efflux.

These results support the hypothesis that hephaestin is involved in iron mobilization of iron from the intestine to circulation.
Title: Iron repletion relocalizes hephaestin to a proximal basolateral compartment in polarized MDCK and Caco2 cells.

Heph is active in placenta but may not play a key role in placental iron transport.
Title: Ferroportin 1 and hephaestin expression in BeWo cell line with different iron treatment.

In contrast to ceruloplasmin, hephaestin was incapable of direct oxidation of adrenaline and dopamine implying a difference in biological substrate specificities between these two homologous ferroxidases.
Title: Oxidation of organic and biogenic amines by recombinant human hephaestin expressed in Pichia pastoris.

The following HEPH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEPH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12228	NM_138737.5 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu21476	NM_001367242.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu21476	NM_014799.4 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu11224	NM_001282141.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu99554	NM_001367243.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu99554	XM_017030003.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu54271	NM_001367232.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu54271	NM_001367233.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu54271	NM_001367234.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu54271	XM_006724722.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu107161	NM_001367236.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99550	NM_001367238.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99551	NM_001367239.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99552	NM_001367240.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99553	NM_001367241.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99549	XM_017029998.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99550	XM_017029999.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99551	XM_017030000.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99552	XM_017030001.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99553	XM_017030002.1 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu11051	NM_001130860.4 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu63368	XM_011531073.3 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu63369	XM_011531074.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu63370	XM_011531075.2 Latest version!	Homo sapiens hephaestin (HEPH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu107161	NM_001367236.1	Homo sapiens hephaestin (HEPH), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu99549	XM_017029998.1	Homo sapiens hephaestin (HEPH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu54271	NM_001367233.1	Homo sapiens hephaestin (HEPH), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu21476	NM_001367242.1	Homo sapiens hephaestin (HEPH), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu99554	NM_001367243.1	Homo sapiens hephaestin (HEPH), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu11224	NM_001282141.1	Homo sapiens hephaestin (HEPH), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu99550	NM_001367238.1	Homo sapiens hephaestin (HEPH), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu54271	NM_001367232.1	Homo sapiens hephaestin (HEPH), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu21476	NM_014799.3	Homo sapiens hephaestin (HEPH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu63368	XM_011531073.2	Homo sapiens hephaestin (HEPH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu12228	NM_138737.4	Homo sapiens hephaestin (HEPH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$853.30 $1219.00
OHu11051	NM_001130860.3	Homo sapiens hephaestin (HEPH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu99552	NM_001367240.1	Homo sapiens hephaestin (HEPH), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEPH ( NM_138737.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_620074.3 Homo sapiens hephaestin (HEPH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12228
Clone ID Related Accession (Same CDS sequence)	NM_138737.4 , NM_138737.5
Accession Version	NM_138737.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	hephaestin isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP282214.1, AL030998.1, BC011561.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_138737.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGGTGAG GTGGTCACTT ATCAGTGGAA CATCCCAGAG 
AGGTCTGGCC CTGGGCCCAA TGACTCTGCT TGTGTTTCCT GGATCTATTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA TAGTGGCCTG GTGGGGCCCT TGGCTATCTG CCAAAAGGGC 
ATCCTGGAGC CCCATGGAGG ACGGAGTGAC ATGGATCGGG AATTTGCATT GTTGTTCTTG 
ATTTTTGATG AAAATAAGTC TTGGTATTTG GAGGAAAATG TGGCAACCCA TGGGTCCCAG 
GATCCAGGCA GTATTAACCT ACAGGATGAA ACTTTCTTGG AGAGCAATAA AATGCATGCA 
ATCAATGGGA AACTCTATGC CAACCTTAGG GGTCTTACCA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGCTGGCCAT GGGCCAAGAT GTGGATCTAC ACACCATCCA CTTTCATGCA 
GAGAGCTTCC TCTATCGGAA TGGCGAGAAC TACCGGGCAG ATGTGGTGGA TCTGTTCCCA 
GGGACTTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GATGCACTGC 
CATGTGACTG ACCATGTCCA TGCTGGCATG GAGACCCTCT TCACTGTTTT TTCTCGAACA 
GAACACTTAA GCCCTCTCAC CGTCATCACC AAAGAGACTG AAAAAGCAGT GCCCCCCAGA 
GACATTGAAG AAGGCAATGT GAAGATGCTG GGCATGCAGA TCCCCATAAA GAATGTTGAG 
ATGCTGGCCT CTGTTTTGGT TGCCATTAGT GTCACCCTTC TGCTCGTTGT TCTGGCTCTT 
GGTGGAGTGG TTTGGTACCA ACATCGACAG AGAAAGCTAC GACGCAATAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTTTC AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620074.3
CDS	58..3696
Translation	MTQTLPYHLSLLNVLFPGPCSRHFKFRRGKCAQPAWRKVSAPSQDLLITKVMWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_138737.5
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138737.5

ORF Insert Sequence:

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGGTGAG GTGGTCACTT ATCAGTGGAA CATCCCAGAG 
AGGTCTGGCC CTGGGCCCAA TGACTCTGCT TGTGTTTCCT GGATCTATTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA TAGTGGCCTG GTGGGGCCCT TGGCTATCTG CCAAAAGGGC 
ATCCTGGAGC CCCATGGAGG ACGGAGTGAC ATGGATCGGG AATTTGCATT GTTGTTCTTG 
ATTTTTGATG AAAATAAGTC TTGGTATTTG GAGGAAAATG TGGCAACCCA TGGGTCCCAG 
GATCCAGGCA GTATTAACCT ACAGGATGAA ACTTTCTTGG AGAGCAATAA AATGCATGCA 
ATCAATGGGA AACTCTATGC CAACCTTAGG GGTCTTACCA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGCTGGCCAT GGGCCAAGAT GTGGATCTAC ACACCATCCA CTTTCATGCA 
GAGAGCTTCC TCTATCGGAA TGGCGAGAAC TACCGGGCAG ATGTGGTGGA TCTGTTCCCA 
GGGACTTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GATGCACTGC 
CATGTGACTG ACCATGTCCA TGCTGGCATG GAGACCCTCT TCACTGTTTT TTCTCGAACA 
GAACACTTAA GCCCTCTCAC CGTCATCACC AAAGAGACTG AAAAAGCAGT GCCCCCCAGA 
GACATTGAAG AAGGCAATGT GAAGATGCTG GGCATGCAGA TCCCCATAAA GAATGTTGAG 
ATGCTGGCCT CTGTTTTGGT TGCCATTAGT GTCACCCTTC TGCTCGTTGT TCTGGCTCTT 
GGTGGAGTGG TTTGGTACCA ACATCGACAG AGAAAGCTAC GACGCAATAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTTTC AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_014799.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055614.1 Homo sapiens hephaestin (HEPH), transcript variant 2, mRNA.

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CloneID	OHu21476
Clone ID Related Accession (Same CDS sequence)	NM_014799.3 , NM_001367242.2 , NM_001367242.1 , NM_014799.4
Accession Version	NM_014799.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	hephaestin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, CN361718.1, AB014598.2 and BC011561.1. This sequence is a reference standard in the RefSeqGene project. On Aug 20, 2013 this sequence version replaced NM_014799.2. Transcript Variant: This variant (2) differs in its 5' UTR, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2 and 13 both encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB014598.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGCAA TCAATGGCTT TGTTTTTGGG AATTTACCTG AGCTGAACAT GTGTGCACAG 
AAACGTGTGG CCTGGCACTT GTTTGGCATG GGCAATGAAA TTGATGTCCA CACAGCATTT 
TTCCATGGAC AGATGCTGAC TACCCGTGGA CACCACACTG ATGTGGCTAA CATCTTTCCA 
GCCACCTTTG TGACTGCTGA GATGGTGCCC TGGGAACCTG GTACCTGGTT AATTAGCTGC 
CAAGTGAACA GTCACTTTCG AGATGGCATG CAGGCACTCT ACAAGGTCAA GTCTTGCTCC 
ATGGCCCCTC CTGTGGACCT GCTCACAGGC AAAGTTCGAC AGTACTTCAT TGAGGCCCAT 
GAGATTCAAT GGGACTATGG CCCGATGGGG CATGATGGGA GTACTGGGAA GAATTTGAGA 
GAGCCAGGCA GTATCTCAGA TAAGTTTTTC CAGAAGAGCT CCAGCCGAAT TGGGGGCACT 
TACTGGAAAG TGCGATATGA AGCCTTTCAA GATGAGACAT TCCAAGAGAA GATGCATTTG 
GAGGAAGATA GGCATCTTGG AATCCTGGGG CCAGTGATCC GGGCTGAGGT GGGTGACACC 
ATTCAGGTGG TCTTCTACAA CCGTGCCTCC CAGCCATTCA GCATGCAGCC CCATGGGGTC 
TTTTATGAGA AAGACTATGA AGGCACTGTG TACAATGATG GCTCATCTTA CCCTGGCTTG 
GTTGCCAAGC CCTTTGAGAA AGTAACATAC CGCTGGACAG TCCCCCCTCA TGCCGGTCCC 
ACTGCTCAGG ATCCTGCTTG TCTCACTTGG ATGTACTTCT CTGCTGCAGA TCCCATAAGA 
GACACAAATT CTGGCCTGGT GGGCCCGCTG CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA 
GATGGCAAGC AGAAAGGGGT GGATAAAGAA TTCTTTCTTC TCTTCACTGT GTTGGATGAG 
AACAAGAGCT GGTACAGCAA TGCCAATCAA GCAGCTGCTA TGTTGGATTT CCGACTGCTT 
TCAGAGGATA TTGAGGGCTT CCAAGACTCC AATCGGATGC ATGCCATTAA TGGGTTTCTG 
TTCTCTAACC TGCCCAGGCT GGACATGTGC AAGGGTGACA CAGTGGCCTG GCACCTGCTC 
GGCCTGGGCA CAGAGACTGA TGTGCATGGA GTCATGTTCC AGGGCAACAC TGTGCAGCTT 
CAGGGCATGA GGAAGGGTGC AGCTATGCTC TTTCCTCATA CCTTTGTCAT GGCCATCATG 
CAGCCTGACA ACCTTGGGAC ATTTGAGATT TATTGCCAGG CAGGCAGCCA TCGAGAAGCA 
GGGATGAGGG CAATCTATAA TGTCTCCCAG TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA 
CGCTACCAAG CTGCAAGAAT CTACTATATC ATGGCAGAAG AAGTAGAGTG GGACTATTGC 
CCTGACCGGA GCTGGGAACG GGAATGGCAC AACCAGTCTG AGAAGGACAG TTATGGTTAC 
ATTTTCCTGA GCAACAAGGA TGGGCTCCTG GGTTCCAGAT ACAAGAAAGC TGTATTCAGG 
GAATACACTG ATGGTACATT CAGGATCCCT CGGCCAAGGA CTGGACCAGA AGAACACTTG 
GGAATCTTGG GTCCACTTAT CAAAGGTGAA GTTGGTGATA TCCTGACTGT GGTATTCAAG 
AATAATGCCA GCCGCCCCTA CTCTGTGCAT GCTCATGGAG TGCTAGAATC TACTACTGTC 
TGGCCACTGG CTGCTGAGCC TGGTGAGGTG GTCACTTATC AGTGGAACAT CCCAGAGAGG 
TCTGGCCCTG GGCCCAATGA CTCTGCTTGT GTTTCCTGGA TCTATTATTC TGCAGTGGAT 
CCCATCAAGG ACATGTATAG TGGCCTGGTG GGGCCCTTGG CTATCTGCCA AAAGGGCATC 
CTGGAGCCCC ATGGAGGACG GAGTGACATG GATCGGGAAT TTGCATTGTT GTTCTTGATT 
TTTGATGAAA ATAAGTCTTG GTATTTGGAG GAAAATGTGG CAACCCATGG GTCCCAGGAT 
CCAGGCAGTA TTAACCTACA GGATGAAACT TTCTTGGAGA GCAATAAAAT GCATGCAATC 
AATGGGAAAC TCTATGCCAA CCTTAGGGGT CTTACCATGT ACCAAGGAGA ACGAGTGGCC 
TGGTACATGC TGGCCATGGG CCAAGATGTG GATCTACACA CCATCCACTT TCATGCAGAG 
AGCTTCCTCT ATCGGAATGG CGAGAACTAC CGGGCAGATG TGGTGGATCT GTTCCCAGGG 
ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC AACCCTGGGA CATGGCTGAT GCACTGCCAT 
GTGACTGACC ATGTCCATGC TGGCATGGAG ACCCTCTTCA CTGTTTTTTC TCGAACAGAA 
CACTTAAGCC CTCTCACCGT CATCACCAAA GAGACTGAAA AAGCAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055614.1
CDS	876..3551
Translation	MHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_014799.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014799.3

ORF Insert Sequence:

ATGCATGCAA TCAATGGCTT TGTTTTTGGG AATTTACCTG AGCTGAACAT GTGTGCACAG 
AAACGTGTGG CCTGGCACTT GTTTGGCATG GGCAATGAAA TTGATGTCCA CACAGCATTT 
TTCCATGGAC AGATGCTGAC TACCCGTGGA CACCACACTG ATGTGGCTAA CATCTTTCCA 
GCCACCTTTG TGACTGCTGA GATGGTGCCC TGGGAACCTG GTACCTGGTT AATTAGCTGC 
CAAGTGAACA GTCACTTTCG AGATGGCATG CAGGCACTCT ACAAGGTCAA GTCTTGCTCC 
ATGGCCCCTC CTGTGGACCT GCTCACAGGC AAAGTTCGAC AGTACTTCAT TGAGGCCCAT 
GAGATTCAAT GGGACTATGG CCCGATGGGG CATGATGGGA GTACTGGGAA GAATTTGAGA 
GAGCCAGGCA GTATCTCAGA TAAGTTTTTC CAGAAGAGCT CCAGCCGAAT TGGGGGCACT 
TACTGGAAAG TGCGATATGA AGCCTTTCAA GATGAGACAT TCCAAGAGAA GATGCATTTG 
GAGGAAGATA GGCATCTTGG AATCCTGGGG CCAGTGATCC GGGCTGAGGT GGGTGACACC 
ATTCAGGTGG TCTTCTACAA CCGTGCCTCC CAGCCATTCA GCATGCAGCC CCATGGGGTC 
TTTTATGAGA AAGACTATGA AGGCACTGTG TACAATGATG GCTCATCTTA CCCTGGCTTG 
GTTGCCAAGC CCTTTGAGAA AGTAACATAC CGCTGGACAG TCCCCCCTCA TGCCGGTCCC 
ACTGCTCAGG ATCCTGCTTG TCTCACTTGG ATGTACTTCT CTGCTGCAGA TCCCATAAGA 
GACACAAATT CTGGCCTGGT GGGCCCGCTG CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA 
GATGGCAAGC AGAAAGGGGT GGATAAAGAA TTCTTTCTTC TCTTCACTGT GTTGGATGAG 
AACAAGAGCT GGTACAGCAA TGCCAATCAA GCAGCTGCTA TGTTGGATTT CCGACTGCTT 
TCAGAGGATA TTGAGGGCTT CCAAGACTCC AATCGGATGC ATGCCATTAA TGGGTTTCTG 
TTCTCTAACC TGCCCAGGCT GGACATGTGC AAGGGTGACA CAGTGGCCTG GCACCTGCTC 
GGCCTGGGCA CAGAGACTGA TGTGCATGGA GTCATGTTCC AGGGCAACAC TGTGCAGCTT 
CAGGGCATGA GGAAGGGTGC AGCTATGCTC TTTCCTCATA CCTTTGTCAT GGCCATCATG 
CAGCCTGACA ACCTTGGGAC ATTTGAGATT TATTGCCAGG CAGGCAGCCA TCGAGAAGCA 
GGGATGAGGG CAATCTATAA TGTCTCCCAG TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA 
CGCTACCAAG CTGCAAGAAT CTACTATATC ATGGCAGAAG AAGTAGAGTG GGACTATTGC 
CCTGACCGGA GCTGGGAACG GGAATGGCAC AACCAGTCTG AGAAGGACAG TTATGGTTAC 
ATTTTCCTGA GCAACAAGGA TGGGCTCCTG GGTTCCAGAT ACAAGAAAGC TGTATTCAGG 
GAATACACTG ATGGTACATT CAGGATCCCT CGGCCAAGGA CTGGACCAGA AGAACACTTG 
GGAATCTTGG GTCCACTTAT CAAAGGTGAA GTTGGTGATA TCCTGACTGT GGTATTCAAG 
AATAATGCCA GCCGCCCCTA CTCTGTGCAT GCTCATGGAG TGCTAGAATC TACTACTGTC 
TGGCCACTGG CTGCTGAGCC TGGTGAGGTG GTCACTTATC AGTGGAACAT CCCAGAGAGG 
TCTGGCCCTG GGCCCAATGA CTCTGCTTGT GTTTCCTGGA TCTATTATTC TGCAGTGGAT 
CCCATCAAGG ACATGTATAG TGGCCTGGTG GGGCCCTTGG CTATCTGCCA AAAGGGCATC 
CTGGAGCCCC ATGGAGGACG GAGTGACATG GATCGGGAAT TTGCATTGTT GTTCTTGATT 
TTTGATGAAA ATAAGTCTTG GTATTTGGAG GAAAATGTGG CAACCCATGG GTCCCAGGAT 
CCAGGCAGTA TTAACCTACA GGATGAAACT TTCTTGGAGA GCAATAAAAT GCATGCAATC 
AATGGGAAAC TCTATGCCAA CCTTAGGGGT CTTACCATGT ACCAAGGAGA ACGAGTGGCC 
TGGTACATGC TGGCCATGGG CCAAGATGTG GATCTACACA CCATCCACTT TCATGCAGAG 
AGCTTCCTCT ATCGGAATGG CGAGAACTAC CGGGCAGATG TGGTGGATCT GTTCCCAGGG 
ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC AACCCTGGGA CATGGCTGAT GCACTGCCAT 
GTGACTGACC ATGTCCATGC TGGCATGGAG ACCCTCTTCA CTGTTTTTTC TCGAACAGAA 
CACTTAAGCC CTCTCACCGT CATCACCAAA GAGACTGAAA AAGCAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367242.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354171.1 Homo sapiens hephaestin (HEPH), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu21476
Clone ID Related Accession (Same CDS sequence)	NM_014799.3 , NM_001367242.2 , NM_001367242.1 , NM_014799.4
Accession Version	NM_001367242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	hephaestin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_001367242.1. Transcript Variant: This variant (13), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.142173.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGCAA TCAATGGCTT TGTTTTTGGG AATTTACCTG AGCTGAACAT GTGTGCACAG 
AAACGTGTGG CCTGGCACTT GTTTGGCATG GGCAATGAAA TTGATGTCCA CACAGCATTT 
TTCCATGGAC AGATGCTGAC TACCCGTGGA CACCACACTG ATGTGGCTAA CATCTTTCCA 
GCCACCTTTG TGACTGCTGA GATGGTGCCC TGGGAACCTG GTACCTGGTT AATTAGCTGC 
CAAGTGAACA GTCACTTTCG AGATGGCATG CAGGCACTCT ACAAGGTCAA GTCTTGCTCC 
ATGGCCCCTC CTGTGGACCT GCTCACAGGC AAAGTTCGAC AGTACTTCAT TGAGGCCCAT 
GAGATTCAAT GGGACTATGG CCCGATGGGG CATGATGGGA GTACTGGGAA GAATTTGAGA 
GAGCCAGGCA GTATCTCAGA TAAGTTTTTC CAGAAGAGCT CCAGCCGAAT TGGGGGCACT 
TACTGGAAAG TGCGATATGA AGCCTTTCAA GATGAGACAT TCCAAGAGAA GATGCATTTG 
GAGGAAGATA GGCATCTTGG AATCCTGGGG CCAGTGATCC GGGCTGAGGT GGGTGACACC 
ATTCAGGTGG TCTTCTACAA CCGTGCCTCC CAGCCATTCA GCATGCAGCC CCATGGGGTC 
TTTTATGAGA AAGACTATGA AGGCACTGTG TACAATGATG GCTCATCTTA CCCTGGCTTG 
GTTGCCAAGC CCTTTGAGAA AGTAACATAC CGCTGGACAG TCCCCCCTCA TGCCGGTCCC 
ACTGCTCAGG ATCCTGCTTG TCTCACTTGG ATGTACTTCT CTGCTGCAGA TCCCATAAGA 
GACACAAATT CTGGCCTGGT GGGCCCGCTG CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA 
GATGGCAAGC AGAAAGGGGT GGATAAAGAA TTCTTTCTTC TCTTCACTGT GTTGGATGAG 
AACAAGAGCT GGTACAGCAA TGCCAATCAA GCAGCTGCTA TGTTGGATTT CCGACTGCTT 
TCAGAGGATA TTGAGGGCTT CCAAGACTCC AATCGGATGC ATGCCATTAA TGGGTTTCTG 
TTCTCTAACC TGCCCAGGCT GGACATGTGC AAGGGTGACA CAGTGGCCTG GCACCTGCTC 
GGCCTGGGCA CAGAGACTGA TGTGCATGGA GTCATGTTCC AGGGCAACAC TGTGCAGCTT 
CAGGGCATGA GGAAGGGTGC AGCTATGCTC TTTCCTCATA CCTTTGTCAT GGCCATCATG 
CAGCCTGACA ACCTTGGGAC ATTTGAGATT TATTGCCAGG CAGGCAGCCA TCGAGAAGCA 
GGGATGAGGG CAATCTATAA TGTCTCCCAG TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA 
CGCTACCAAG CTGCAAGAAT CTACTATATC ATGGCAGAAG AAGTAGAGTG GGACTATTGC 
CCTGACCGGA GCTGGGAACG GGAATGGCAC AACCAGTCTG AGAAGGACAG TTATGGTTAC 
ATTTTCCTGA GCAACAAGGA TGGGCTCCTG GGTTCCAGAT ACAAGAAAGC TGTATTCAGG 
GAATACACTG ATGGTACATT CAGGATCCCT CGGCCAAGGA CTGGACCAGA AGAACACTTG 
GGAATCTTGG GTCCACTTAT CAAAGGTGAA GTTGGTGATA TCCTGACTGT GGTATTCAAG 
AATAATGCCA GCCGCCCCTA CTCTGTGCAT GCTCATGGAG TGCTAGAATC TACTACTGTC 
TGGCCACTGG CTGCTGAGCC TGGTGAGGTG GTCACTTATC AGTGGAACAT CCCAGAGAGG 
TCTGGCCCTG GGCCCAATGA CTCTGCTTGT GTTTCCTGGA TCTATTATTC TGCAGTGGAT 
CCCATCAAGG ACATGTATAG TGGCCTGGTG GGGCCCTTGG CTATCTGCCA AAAGGGCATC 
CTGGAGCCCC ATGGAGGACG GAGTGACATG GATCGGGAAT TTGCATTGTT GTTCTTGATT 
TTTGATGAAA ATAAGTCTTG GTATTTGGAG GAAAATGTGG CAACCCATGG GTCCCAGGAT 
CCAGGCAGTA TTAACCTACA GGATGAAACT TTCTTGGAGA GCAATAAAAT GCATGCAATC 
AATGGGAAAC TCTATGCCAA CCTTAGGGGT CTTACCATGT ACCAAGGAGA ACGAGTGGCC 
TGGTACATGC TGGCCATGGG CCAAGATGTG GATCTACACA CCATCCACTT TCATGCAGAG 
AGCTTCCTCT ATCGGAATGG CGAGAACTAC CGGGCAGATG TGGTGGATCT GTTCCCAGGG 
ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC AACCCTGGGA CATGGCTGAT GCACTGCCAT 
GTGACTGACC ATGTCCATGC TGGCATGGAG ACCCTCTTCA CTGTTTTTTC TCGAACAGAA 
CACTTAAGCC CTCTCACCGT CATCACCAAA GAGACTGAAA AAGCAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354171.1
CDS	1257..3932
Translation	MHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367242.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367242.2

ORF Insert Sequence:

ATGCATGCAA TCAATGGCTT TGTTTTTGGG AATTTACCTG AGCTGAACAT GTGTGCACAG 
AAACGTGTGG CCTGGCACTT GTTTGGCATG GGCAATGAAA TTGATGTCCA CACAGCATTT 
TTCCATGGAC AGATGCTGAC TACCCGTGGA CACCACACTG ATGTGGCTAA CATCTTTCCA 
GCCACCTTTG TGACTGCTGA GATGGTGCCC TGGGAACCTG GTACCTGGTT AATTAGCTGC 
CAAGTGAACA GTCACTTTCG AGATGGCATG CAGGCACTCT ACAAGGTCAA GTCTTGCTCC 
ATGGCCCCTC CTGTGGACCT GCTCACAGGC AAAGTTCGAC AGTACTTCAT TGAGGCCCAT 
GAGATTCAAT GGGACTATGG CCCGATGGGG CATGATGGGA GTACTGGGAA GAATTTGAGA 
GAGCCAGGCA GTATCTCAGA TAAGTTTTTC CAGAAGAGCT CCAGCCGAAT TGGGGGCACT 
TACTGGAAAG TGCGATATGA AGCCTTTCAA GATGAGACAT TCCAAGAGAA GATGCATTTG 
GAGGAAGATA GGCATCTTGG AATCCTGGGG CCAGTGATCC GGGCTGAGGT GGGTGACACC 
ATTCAGGTGG TCTTCTACAA CCGTGCCTCC CAGCCATTCA GCATGCAGCC CCATGGGGTC 
TTTTATGAGA AAGACTATGA AGGCACTGTG TACAATGATG GCTCATCTTA CCCTGGCTTG 
GTTGCCAAGC CCTTTGAGAA AGTAACATAC CGCTGGACAG TCCCCCCTCA TGCCGGTCCC 
ACTGCTCAGG ATCCTGCTTG TCTCACTTGG ATGTACTTCT CTGCTGCAGA TCCCATAAGA 
GACACAAATT CTGGCCTGGT GGGCCCGCTG CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA 
GATGGCAAGC AGAAAGGGGT GGATAAAGAA TTCTTTCTTC TCTTCACTGT GTTGGATGAG 
AACAAGAGCT GGTACAGCAA TGCCAATCAA GCAGCTGCTA TGTTGGATTT CCGACTGCTT 
TCAGAGGATA TTGAGGGCTT CCAAGACTCC AATCGGATGC ATGCCATTAA TGGGTTTCTG 
TTCTCTAACC TGCCCAGGCT GGACATGTGC AAGGGTGACA CAGTGGCCTG GCACCTGCTC 
GGCCTGGGCA CAGAGACTGA TGTGCATGGA GTCATGTTCC AGGGCAACAC TGTGCAGCTT 
CAGGGCATGA GGAAGGGTGC AGCTATGCTC TTTCCTCATA CCTTTGTCAT GGCCATCATG 
CAGCCTGACA ACCTTGGGAC ATTTGAGATT TATTGCCAGG CAGGCAGCCA TCGAGAAGCA 
GGGATGAGGG CAATCTATAA TGTCTCCCAG TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA 
CGCTACCAAG CTGCAAGAAT CTACTATATC ATGGCAGAAG AAGTAGAGTG GGACTATTGC 
CCTGACCGGA GCTGGGAACG GGAATGGCAC AACCAGTCTG AGAAGGACAG TTATGGTTAC 
ATTTTCCTGA GCAACAAGGA TGGGCTCCTG GGTTCCAGAT ACAAGAAAGC TGTATTCAGG 
GAATACACTG ATGGTACATT CAGGATCCCT CGGCCAAGGA CTGGACCAGA AGAACACTTG 
GGAATCTTGG GTCCACTTAT CAAAGGTGAA GTTGGTGATA TCCTGACTGT GGTATTCAAG 
AATAATGCCA GCCGCCCCTA CTCTGTGCAT GCTCATGGAG TGCTAGAATC TACTACTGTC 
TGGCCACTGG CTGCTGAGCC TGGTGAGGTG GTCACTTATC AGTGGAACAT CCCAGAGAGG 
TCTGGCCCTG GGCCCAATGA CTCTGCTTGT GTTTCCTGGA TCTATTATTC TGCAGTGGAT 
CCCATCAAGG ACATGTATAG TGGCCTGGTG GGGCCCTTGG CTATCTGCCA AAAGGGCATC 
CTGGAGCCCC ATGGAGGACG GAGTGACATG GATCGGGAAT TTGCATTGTT GTTCTTGATT 
TTTGATGAAA ATAAGTCTTG GTATTTGGAG GAAAATGTGG CAACCCATGG GTCCCAGGAT 
CCAGGCAGTA TTAACCTACA GGATGAAACT TTCTTGGAGA GCAATAAAAT GCATGCAATC 
AATGGGAAAC TCTATGCCAA CCTTAGGGGT CTTACCATGT ACCAAGGAGA ACGAGTGGCC 
TGGTACATGC TGGCCATGGG CCAAGATGTG GATCTACACA CCATCCACTT TCATGCAGAG 
AGCTTCCTCT ATCGGAATGG CGAGAACTAC CGGGCAGATG TGGTGGATCT GTTCCCAGGG 
ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC AACCCTGGGA CATGGCTGAT GCACTGCCAT 
GTGACTGACC ATGTCCATGC TGGCATGGAG ACCCTCTTCA CTGTTTTTTC TCGAACAGAA 
CACTTAAGCC CTCTCACCGT CATCACCAAA GAGACTGAAA AAGCAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367242.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354171.1 Homo sapiens hephaestin (HEPH), transcript variant 13, mRNA.

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CloneID	OHu21476
Clone ID Related Accession (Same CDS sequence)	NM_014799.3 , NM_001367242.2 , NM_001367242.1 , NM_014799.4
Accession Version	NM_001367242.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	hephaestin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. Transcript Variant: This variant (13), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.142173.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGCAA TCAATGGCTT TGTTTTTGGG AATTTACCTG AGCTGAACAT GTGTGCACAG 
AAACGTGTGG CCTGGCACTT GTTTGGCATG GGCAATGAAA TTGATGTCCA CACAGCATTT 
TTCCATGGAC AGATGCTGAC TACCCGTGGA CACCACACTG ATGTGGCTAA CATCTTTCCA 
GCCACCTTTG TGACTGCTGA GATGGTGCCC TGGGAACCTG GTACCTGGTT AATTAGCTGC 
CAAGTGAACA GTCACTTTCG AGATGGCATG CAGGCACTCT ACAAGGTCAA GTCTTGCTCC 
ATGGCCCCTC CTGTGGACCT GCTCACAGGC AAAGTTCGAC AGTACTTCAT TGAGGCCCAT 
GAGATTCAAT GGGACTATGG CCCGATGGGG CATGATGGGA GTACTGGGAA GAATTTGAGA 
GAGCCAGGCA GTATCTCAGA TAAGTTTTTC CAGAAGAGCT CCAGCCGAAT TGGGGGCACT 
TACTGGAAAG TGCGATATGA AGCCTTTCAA GATGAGACAT TCCAAGAGAA GATGCATTTG 
GAGGAAGATA GGCATCTTGG AATCCTGGGG CCAGTGATCC GGGCTGAGGT GGGTGACACC 
ATTCAGGTGG TCTTCTACAA CCGTGCCTCC CAGCCATTCA GCATGCAGCC CCATGGGGTC 
TTTTATGAGA AAGACTATGA AGGCACTGTG TACAATGATG GCTCATCTTA CCCTGGCTTG 
GTTGCCAAGC CCTTTGAGAA AGTAACATAC CGCTGGACAG TCCCCCCTCA TGCCGGTCCC 
ACTGCTCAGG ATCCTGCTTG TCTCACTTGG ATGTACTTCT CTGCTGCAGA TCCCATAAGA 
GACACAAATT CTGGCCTGGT GGGCCCGCTG CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA 
GATGGCAAGC AGAAAGGGGT GGATAAAGAA TTCTTTCTTC TCTTCACTGT GTTGGATGAG 
AACAAGAGCT GGTACAGCAA TGCCAATCAA GCAGCTGCTA TGTTGGATTT CCGACTGCTT 
TCAGAGGATA TTGAGGGCTT CCAAGACTCC AATCGGATGC ATGCCATTAA TGGGTTTCTG 
TTCTCTAACC TGCCCAGGCT GGACATGTGC AAGGGTGACA CAGTGGCCTG GCACCTGCTC 
GGCCTGGGCA CAGAGACTGA TGTGCATGGA GTCATGTTCC AGGGCAACAC TGTGCAGCTT 
CAGGGCATGA GGAAGGGTGC AGCTATGCTC TTTCCTCATA CCTTTGTCAT GGCCATCATG 
CAGCCTGACA ACCTTGGGAC ATTTGAGATT TATTGCCAGG CAGGCAGCCA TCGAGAAGCA 
GGGATGAGGG CAATCTATAA TGTCTCCCAG TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA 
CGCTACCAAG CTGCAAGAAT CTACTATATC ATGGCAGAAG AAGTAGAGTG GGACTATTGC 
CCTGACCGGA GCTGGGAACG GGAATGGCAC AACCAGTCTG AGAAGGACAG TTATGGTTAC 
ATTTTCCTGA GCAACAAGGA TGGGCTCCTG GGTTCCAGAT ACAAGAAAGC TGTATTCAGG 
GAATACACTG ATGGTACATT CAGGATCCCT CGGCCAAGGA CTGGACCAGA AGAACACTTG 
GGAATCTTGG GTCCACTTAT CAAAGGTGAA GTTGGTGATA TCCTGACTGT GGTATTCAAG 
AATAATGCCA GCCGCCCCTA CTCTGTGCAT GCTCATGGAG TGCTAGAATC TACTACTGTC 
TGGCCACTGG CTGCTGAGCC TGGTGAGGTG GTCACTTATC AGTGGAACAT CCCAGAGAGG 
TCTGGCCCTG GGCCCAATGA CTCTGCTTGT GTTTCCTGGA TCTATTATTC TGCAGTGGAT 
CCCATCAAGG ACATGTATAG TGGCCTGGTG GGGCCCTTGG CTATCTGCCA AAAGGGCATC 
CTGGAGCCCC ATGGAGGACG GAGTGACATG GATCGGGAAT TTGCATTGTT GTTCTTGATT 
TTTGATGAAA ATAAGTCTTG GTATTTGGAG GAAAATGTGG CAACCCATGG GTCCCAGGAT 
CCAGGCAGTA TTAACCTACA GGATGAAACT TTCTTGGAGA GCAATAAAAT GCATGCAATC 
AATGGGAAAC TCTATGCCAA CCTTAGGGGT CTTACCATGT ACCAAGGAGA ACGAGTGGCC 
TGGTACATGC TGGCCATGGG CCAAGATGTG GATCTACACA CCATCCACTT TCATGCAGAG 
AGCTTCCTCT ATCGGAATGG CGAGAACTAC CGGGCAGATG TGGTGGATCT GTTCCCAGGG 
ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC AACCCTGGGA CATGGCTGAT GCACTGCCAT 
GTGACTGACC ATGTCCATGC TGGCATGGAG ACCCTCTTCA CTGTTTTTTC TCGAACAGAA 
CACTTAAGCC CTCTCACCGT CATCACCAAA GAGACTGAAA AAGCAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354171.1
CDS	1257..3932
Translation	MHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367242.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367242.1

ORF Insert Sequence:

ATGCATGCAA TCAATGGCTT TGTTTTTGGG AATTTACCTG AGCTGAACAT GTGTGCACAG 
AAACGTGTGG CCTGGCACTT GTTTGGCATG GGCAATGAAA TTGATGTCCA CACAGCATTT 
TTCCATGGAC AGATGCTGAC TACCCGTGGA CACCACACTG ATGTGGCTAA CATCTTTCCA 
GCCACCTTTG TGACTGCTGA GATGGTGCCC TGGGAACCTG GTACCTGGTT AATTAGCTGC 
CAAGTGAACA GTCACTTTCG AGATGGCATG CAGGCACTCT ACAAGGTCAA GTCTTGCTCC 
ATGGCCCCTC CTGTGGACCT GCTCACAGGC AAAGTTCGAC AGTACTTCAT TGAGGCCCAT 
GAGATTCAAT GGGACTATGG CCCGATGGGG CATGATGGGA GTACTGGGAA GAATTTGAGA 
GAGCCAGGCA GTATCTCAGA TAAGTTTTTC CAGAAGAGCT CCAGCCGAAT TGGGGGCACT 
TACTGGAAAG TGCGATATGA AGCCTTTCAA GATGAGACAT TCCAAGAGAA GATGCATTTG 
GAGGAAGATA GGCATCTTGG AATCCTGGGG CCAGTGATCC GGGCTGAGGT GGGTGACACC 
ATTCAGGTGG TCTTCTACAA CCGTGCCTCC CAGCCATTCA GCATGCAGCC CCATGGGGTC 
TTTTATGAGA AAGACTATGA AGGCACTGTG TACAATGATG GCTCATCTTA CCCTGGCTTG 
GTTGCCAAGC CCTTTGAGAA AGTAACATAC CGCTGGACAG TCCCCCCTCA TGCCGGTCCC 
ACTGCTCAGG ATCCTGCTTG TCTCACTTGG ATGTACTTCT CTGCTGCAGA TCCCATAAGA 
GACACAAATT CTGGCCTGGT GGGCCCGCTG CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA 
GATGGCAAGC AGAAAGGGGT GGATAAAGAA TTCTTTCTTC TCTTCACTGT GTTGGATGAG 
AACAAGAGCT GGTACAGCAA TGCCAATCAA GCAGCTGCTA TGTTGGATTT CCGACTGCTT 
TCAGAGGATA TTGAGGGCTT CCAAGACTCC AATCGGATGC ATGCCATTAA TGGGTTTCTG 
TTCTCTAACC TGCCCAGGCT GGACATGTGC AAGGGTGACA CAGTGGCCTG GCACCTGCTC 
GGCCTGGGCA CAGAGACTGA TGTGCATGGA GTCATGTTCC AGGGCAACAC TGTGCAGCTT 
CAGGGCATGA GGAAGGGTGC AGCTATGCTC TTTCCTCATA CCTTTGTCAT GGCCATCATG 
CAGCCTGACA ACCTTGGGAC ATTTGAGATT TATTGCCAGG CAGGCAGCCA TCGAGAAGCA 
GGGATGAGGG CAATCTATAA TGTCTCCCAG TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA 
CGCTACCAAG CTGCAAGAAT CTACTATATC ATGGCAGAAG AAGTAGAGTG GGACTATTGC 
CCTGACCGGA GCTGGGAACG GGAATGGCAC AACCAGTCTG AGAAGGACAG TTATGGTTAC 
ATTTTCCTGA GCAACAAGGA TGGGCTCCTG GGTTCCAGAT ACAAGAAAGC TGTATTCAGG 
GAATACACTG ATGGTACATT CAGGATCCCT CGGCCAAGGA CTGGACCAGA AGAACACTTG 
GGAATCTTGG GTCCACTTAT CAAAGGTGAA GTTGGTGATA TCCTGACTGT GGTATTCAAG 
AATAATGCCA GCCGCCCCTA CTCTGTGCAT GCTCATGGAG TGCTAGAATC TACTACTGTC 
TGGCCACTGG CTGCTGAGCC TGGTGAGGTG GTCACTTATC AGTGGAACAT CCCAGAGAGG 
TCTGGCCCTG GGCCCAATGA CTCTGCTTGT GTTTCCTGGA TCTATTATTC TGCAGTGGAT 
CCCATCAAGG ACATGTATAG TGGCCTGGTG GGGCCCTTGG CTATCTGCCA AAAGGGCATC 
CTGGAGCCCC ATGGAGGACG GAGTGACATG GATCGGGAAT TTGCATTGTT GTTCTTGATT 
TTTGATGAAA ATAAGTCTTG GTATTTGGAG GAAAATGTGG CAACCCATGG GTCCCAGGAT 
CCAGGCAGTA TTAACCTACA GGATGAAACT TTCTTGGAGA GCAATAAAAT GCATGCAATC 
AATGGGAAAC TCTATGCCAA CCTTAGGGGT CTTACCATGT ACCAAGGAGA ACGAGTGGCC 
TGGTACATGC TGGCCATGGG CCAAGATGTG GATCTACACA CCATCCACTT TCATGCAGAG 
AGCTTCCTCT ATCGGAATGG CGAGAACTAC CGGGCAGATG TGGTGGATCT GTTCCCAGGG 
ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC AACCCTGGGA CATGGCTGAT GCACTGCCAT 
GTGACTGACC ATGTCCATGC TGGCATGGAG ACCCTCTTCA CTGTTTTTTC TCGAACAGAA 
CACTTAAGCC CTCTCACCGT CATCACCAAA GAGACTGAAA AAGCAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_014799.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055614.1 Homo sapiens hephaestin (HEPH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21476
Clone ID Related Accession (Same CDS sequence)	NM_014799.3 , NM_001367242.2 , NM_001367242.1 , NM_014799.4
Accession Version	NM_014799.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	hephaestin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN361718.1, AB014598.2, BC011561.1 and AL157698.8. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_014799.3. Transcript Variant: This variant (2) differs in its 5' UTR, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2 and 13 both encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB014598.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGCAA TCAATGGCTT TGTTTTTGGG AATTTACCTG AGCTGAACAT GTGTGCACAG 
AAACGTGTGG CCTGGCACTT GTTTGGCATG GGCAATGAAA TTGATGTCCA CACAGCATTT 
TTCCATGGAC AGATGCTGAC TACCCGTGGA CACCACACTG ATGTGGCTAA CATCTTTCCA 
GCCACCTTTG TGACTGCTGA GATGGTGCCC TGGGAACCTG GTACCTGGTT AATTAGCTGC 
CAAGTGAACA GTCACTTTCG AGATGGCATG CAGGCACTCT ACAAGGTCAA GTCTTGCTCC 
ATGGCCCCTC CTGTGGACCT GCTCACAGGC AAAGTTCGAC AGTACTTCAT TGAGGCCCAT 
GAGATTCAAT GGGACTATGG CCCGATGGGG CATGATGGGA GTACTGGGAA GAATTTGAGA 
GAGCCAGGCA GTATCTCAGA TAAGTTTTTC CAGAAGAGCT CCAGCCGAAT TGGGGGCACT 
TACTGGAAAG TGCGATATGA AGCCTTTCAA GATGAGACAT TCCAAGAGAA GATGCATTTG 
GAGGAAGATA GGCATCTTGG AATCCTGGGG CCAGTGATCC GGGCTGAGGT GGGTGACACC 
ATTCAGGTGG TCTTCTACAA CCGTGCCTCC CAGCCATTCA GCATGCAGCC CCATGGGGTC 
TTTTATGAGA AAGACTATGA AGGCACTGTG TACAATGATG GCTCATCTTA CCCTGGCTTG 
GTTGCCAAGC CCTTTGAGAA AGTAACATAC CGCTGGACAG TCCCCCCTCA TGCCGGTCCC 
ACTGCTCAGG ATCCTGCTTG TCTCACTTGG ATGTACTTCT CTGCTGCAGA TCCCATAAGA 
GACACAAATT CTGGCCTGGT GGGCCCGCTG CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA 
GATGGCAAGC AGAAAGGGGT GGATAAAGAA TTCTTTCTTC TCTTCACTGT GTTGGATGAG 
AACAAGAGCT GGTACAGCAA TGCCAATCAA GCAGCTGCTA TGTTGGATTT CCGACTGCTT 
TCAGAGGATA TTGAGGGCTT CCAAGACTCC AATCGGATGC ATGCCATTAA TGGGTTTCTG 
TTCTCTAACC TGCCCAGGCT GGACATGTGC AAGGGTGACA CAGTGGCCTG GCACCTGCTC 
GGCCTGGGCA CAGAGACTGA TGTGCATGGA GTCATGTTCC AGGGCAACAC TGTGCAGCTT 
CAGGGCATGA GGAAGGGTGC AGCTATGCTC TTTCCTCATA CCTTTGTCAT GGCCATCATG 
CAGCCTGACA ACCTTGGGAC ATTTGAGATT TATTGCCAGG CAGGCAGCCA TCGAGAAGCA 
GGGATGAGGG CAATCTATAA TGTCTCCCAG TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA 
CGCTACCAAG CTGCAAGAAT CTACTATATC ATGGCAGAAG AAGTAGAGTG GGACTATTGC 
CCTGACCGGA GCTGGGAACG GGAATGGCAC AACCAGTCTG AGAAGGACAG TTATGGTTAC 
ATTTTCCTGA GCAACAAGGA TGGGCTCCTG GGTTCCAGAT ACAAGAAAGC TGTATTCAGG 
GAATACACTG ATGGTACATT CAGGATCCCT CGGCCAAGGA CTGGACCAGA AGAACACTTG 
GGAATCTTGG GTCCACTTAT CAAAGGTGAA GTTGGTGATA TCCTGACTGT GGTATTCAAG 
AATAATGCCA GCCGCCCCTA CTCTGTGCAT GCTCATGGAG TGCTAGAATC TACTACTGTC 
TGGCCACTGG CTGCTGAGCC TGGTGAGGTG GTCACTTATC AGTGGAACAT CCCAGAGAGG 
TCTGGCCCTG GGCCCAATGA CTCTGCTTGT GTTTCCTGGA TCTATTATTC TGCAGTGGAT 
CCCATCAAGG ACATGTATAG TGGCCTGGTG GGGCCCTTGG CTATCTGCCA AAAGGGCATC 
CTGGAGCCCC ATGGAGGACG GAGTGACATG GATCGGGAAT TTGCATTGTT GTTCTTGATT 
TTTGATGAAA ATAAGTCTTG GTATTTGGAG GAAAATGTGG CAACCCATGG GTCCCAGGAT 
CCAGGCAGTA TTAACCTACA GGATGAAACT TTCTTGGAGA GCAATAAAAT GCATGCAATC 
AATGGGAAAC TCTATGCCAA CCTTAGGGGT CTTACCATGT ACCAAGGAGA ACGAGTGGCC 
TGGTACATGC TGGCCATGGG CCAAGATGTG GATCTACACA CCATCCACTT TCATGCAGAG 
AGCTTCCTCT ATCGGAATGG CGAGAACTAC CGGGCAGATG TGGTGGATCT GTTCCCAGGG 
ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC AACCCTGGGA CATGGCTGAT GCACTGCCAT 
GTGACTGACC ATGTCCATGC TGGCATGGAG ACCCTCTTCA CTGTTTTTTC TCGAACAGAA 
CACTTAAGCC CTCTCACCGT CATCACCAAA GAGACTGAAA AAGCAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055614.1
CDS	859..3534
Translation	MHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_014799.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014799.4

ORF Insert Sequence:

ATGCATGCAA TCAATGGCTT TGTTTTTGGG AATTTACCTG AGCTGAACAT GTGTGCACAG 
AAACGTGTGG CCTGGCACTT GTTTGGCATG GGCAATGAAA TTGATGTCCA CACAGCATTT 
TTCCATGGAC AGATGCTGAC TACCCGTGGA CACCACACTG ATGTGGCTAA CATCTTTCCA 
GCCACCTTTG TGACTGCTGA GATGGTGCCC TGGGAACCTG GTACCTGGTT AATTAGCTGC 
CAAGTGAACA GTCACTTTCG AGATGGCATG CAGGCACTCT ACAAGGTCAA GTCTTGCTCC 
ATGGCCCCTC CTGTGGACCT GCTCACAGGC AAAGTTCGAC AGTACTTCAT TGAGGCCCAT 
GAGATTCAAT GGGACTATGG CCCGATGGGG CATGATGGGA GTACTGGGAA GAATTTGAGA 
GAGCCAGGCA GTATCTCAGA TAAGTTTTTC CAGAAGAGCT CCAGCCGAAT TGGGGGCACT 
TACTGGAAAG TGCGATATGA AGCCTTTCAA GATGAGACAT TCCAAGAGAA GATGCATTTG 
GAGGAAGATA GGCATCTTGG AATCCTGGGG CCAGTGATCC GGGCTGAGGT GGGTGACACC 
ATTCAGGTGG TCTTCTACAA CCGTGCCTCC CAGCCATTCA GCATGCAGCC CCATGGGGTC 
TTTTATGAGA AAGACTATGA AGGCACTGTG TACAATGATG GCTCATCTTA CCCTGGCTTG 
GTTGCCAAGC CCTTTGAGAA AGTAACATAC CGCTGGACAG TCCCCCCTCA TGCCGGTCCC 
ACTGCTCAGG ATCCTGCTTG TCTCACTTGG ATGTACTTCT CTGCTGCAGA TCCCATAAGA 
GACACAAATT CTGGCCTGGT GGGCCCGCTG CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA 
GATGGCAAGC AGAAAGGGGT GGATAAAGAA TTCTTTCTTC TCTTCACTGT GTTGGATGAG 
AACAAGAGCT GGTACAGCAA TGCCAATCAA GCAGCTGCTA TGTTGGATTT CCGACTGCTT 
TCAGAGGATA TTGAGGGCTT CCAAGACTCC AATCGGATGC ATGCCATTAA TGGGTTTCTG 
TTCTCTAACC TGCCCAGGCT GGACATGTGC AAGGGTGACA CAGTGGCCTG GCACCTGCTC 
GGCCTGGGCA CAGAGACTGA TGTGCATGGA GTCATGTTCC AGGGCAACAC TGTGCAGCTT 
CAGGGCATGA GGAAGGGTGC AGCTATGCTC TTTCCTCATA CCTTTGTCAT GGCCATCATG 
CAGCCTGACA ACCTTGGGAC ATTTGAGATT TATTGCCAGG CAGGCAGCCA TCGAGAAGCA 
GGGATGAGGG CAATCTATAA TGTCTCCCAG TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA 
CGCTACCAAG CTGCAAGAAT CTACTATATC ATGGCAGAAG AAGTAGAGTG GGACTATTGC 
CCTGACCGGA GCTGGGAACG GGAATGGCAC AACCAGTCTG AGAAGGACAG TTATGGTTAC 
ATTTTCCTGA GCAACAAGGA TGGGCTCCTG GGTTCCAGAT ACAAGAAAGC TGTATTCAGG 
GAATACACTG ATGGTACATT CAGGATCCCT CGGCCAAGGA CTGGACCAGA AGAACACTTG 
GGAATCTTGG GTCCACTTAT CAAAGGTGAA GTTGGTGATA TCCTGACTGT GGTATTCAAG 
AATAATGCCA GCCGCCCCTA CTCTGTGCAT GCTCATGGAG TGCTAGAATC TACTACTGTC 
TGGCCACTGG CTGCTGAGCC TGGTGAGGTG GTCACTTATC AGTGGAACAT CCCAGAGAGG 
TCTGGCCCTG GGCCCAATGA CTCTGCTTGT GTTTCCTGGA TCTATTATTC TGCAGTGGAT 
CCCATCAAGG ACATGTATAG TGGCCTGGTG GGGCCCTTGG CTATCTGCCA AAAGGGCATC 
CTGGAGCCCC ATGGAGGACG GAGTGACATG GATCGGGAAT TTGCATTGTT GTTCTTGATT 
TTTGATGAAA ATAAGTCTTG GTATTTGGAG GAAAATGTGG CAACCCATGG GTCCCAGGAT 
CCAGGCAGTA TTAACCTACA GGATGAAACT TTCTTGGAGA GCAATAAAAT GCATGCAATC 
AATGGGAAAC TCTATGCCAA CCTTAGGGGT CTTACCATGT ACCAAGGAGA ACGAGTGGCC 
TGGTACATGC TGGCCATGGG CCAAGATGTG GATCTACACA CCATCCACTT TCATGCAGAG 
AGCTTCCTCT ATCGGAATGG CGAGAACTAC CGGGCAGATG TGGTGGATCT GTTCCCAGGG 
ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC AACCCTGGGA CATGGCTGAT GCACTGCCAT 
GTGACTGACC ATGTCCATGC TGGCATGGAG ACCCTCTTCA CTGTTTTTTC TCGAACAGAA 
CACTTAAGCC CTCTCACCGT CATCACCAAA GAGACTGAAA AAGCAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001282141.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269070.1 Homo sapiens hephaestin (HEPH), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu11224
Clone ID Related Accession (Same CDS sequence)	NM_001282141.1 , NM_001282141.2
Accession Version	NM_001282141.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	hephaestin isoform d precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, CN361718.1, AY358990.1 and BC011561.1. On Aug 31, 2013 this sequence version replaced XM_005262316.1. Transcript Variant: This variant (4) contains an alternate 5'-most exon, lacks three consecutive exons in the central coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (d) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.129562.1, SRR1660809.61476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGGTGA GGTGGTCACT TATCAGTGGA ACATCCCAGA GAGGTCTGGC 
CCTGGGCCCA ATGACTCTGC TTGTGTTTCC TGGATCTATT ATTCTGCAGT GGATCCCATC 
AAGGACATGT ATAGTGGCCT GGTGGGGCCC TTGGCTATCT GCCAAAAGGG CATCCTGGAG 
CCCCATGGAG GACGGAGTGA CATGGATCGG GAATTTGCAT TGTTGTTCTT GATTTTTGAT 
GAAAATAAGT CTTGGTATTT GGAGGAAAAT GTGGCAACCC ATGGGTCCCA GGATCCAGGC 
AGTATTAACC TACAGGATGA AACTTTCTTG GAGAGCAATA AAATGCATGC AATCAATGGG 
AAACTCTATG CCAACCTTAG GGGTCTTACC ATGTACCAAG GAGAACGAGT GGCCTGGTAC 
ATGCTGGCCA TGGGCCAAGA TGTGGATCTA CACACCATCC ACTTTCATGC AGAGAGCTTC 
CTCTATCGGA ATGGCGAGAA CTACCGGGCA GATGTGGTGG ATCTGTTCCC AGGGACTTTT 
GAGGTTGTGG AGATGGTGGC CAGCAACCCT GGGACATGGC TGATGCACTG CCATGTGACT 
GACCATGTCC ATGCTGGCAT GGAGACCCTC TTCACTGTTT TTTCTCGAAC AGAACACTTA 
AGCCCTCTCA CCGTCATCAC CAAAGAGACT GAAAAAGCAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269070.1
CDS	246..3155
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001282141.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282141.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGGTGA GGTGGTCACT TATCAGTGGA ACATCCCAGA GAGGTCTGGC 
CCTGGGCCCA ATGACTCTGC TTGTGTTTCC TGGATCTATT ATTCTGCAGT GGATCCCATC 
AAGGACATGT ATAGTGGCCT GGTGGGGCCC TTGGCTATCT GCCAAAAGGG CATCCTGGAG 
CCCCATGGAG GACGGAGTGA CATGGATCGG GAATTTGCAT TGTTGTTCTT GATTTTTGAT 
GAAAATAAGT CTTGGTATTT GGAGGAAAAT GTGGCAACCC ATGGGTCCCA GGATCCAGGC 
AGTATTAACC TACAGGATGA AACTTTCTTG GAGAGCAATA AAATGCATGC AATCAATGGG 
AAACTCTATG CCAACCTTAG GGGTCTTACC ATGTACCAAG GAGAACGAGT GGCCTGGTAC 
ATGCTGGCCA TGGGCCAAGA TGTGGATCTA CACACCATCC ACTTTCATGC AGAGAGCTTC 
CTCTATCGGA ATGGCGAGAA CTACCGGGCA GATGTGGTGG ATCTGTTCCC AGGGACTTTT 
GAGGTTGTGG AGATGGTGGC CAGCAACCCT GGGACATGGC TGATGCACTG CCATGTGACT 
GACCATGTCC ATGCTGGCAT GGAGACCCTC TTCACTGTTT TTTCTCGAAC AGAACACTTA 
AGCCCTCTCA CCGTCATCAC CAAAGAGACT GAAAAAGCAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001282141.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269070.1 Homo sapiens hephaestin (HEPH), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu11224
Clone ID Related Accession (Same CDS sequence)	NM_001282141.1 , NM_001282141.2
Accession Version	NM_001282141.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	hephaestin isoform d precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN361718.1, AY358990.1, BC011561.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_001282141.1. Transcript Variant: This variant (4) contains an alternate 5'-most exon, lacks three consecutive exons in the central coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (d) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.61476.1, SRR1660807.129562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGGTGA GGTGGTCACT TATCAGTGGA ACATCCCAGA GAGGTCTGGC 
CCTGGGCCCA ATGACTCTGC TTGTGTTTCC TGGATCTATT ATTCTGCAGT GGATCCCATC 
AAGGACATGT ATAGTGGCCT GGTGGGGCCC TTGGCTATCT GCCAAAAGGG CATCCTGGAG 
CCCCATGGAG GACGGAGTGA CATGGATCGG GAATTTGCAT TGTTGTTCTT GATTTTTGAT 
GAAAATAAGT CTTGGTATTT GGAGGAAAAT GTGGCAACCC ATGGGTCCCA GGATCCAGGC 
AGTATTAACC TACAGGATGA AACTTTCTTG GAGAGCAATA AAATGCATGC AATCAATGGG 
AAACTCTATG CCAACCTTAG GGGTCTTACC ATGTACCAAG GAGAACGAGT GGCCTGGTAC 
ATGCTGGCCA TGGGCCAAGA TGTGGATCTA CACACCATCC ACTTTCATGC AGAGAGCTTC 
CTCTATCGGA ATGGCGAGAA CTACCGGGCA GATGTGGTGG ATCTGTTCCC AGGGACTTTT 
GAGGTTGTGG AGATGGTGGC CAGCAACCCT GGGACATGGC TGATGCACTG CCATGTGACT 
GACCATGTCC ATGCTGGCAT GGAGACCCTC TTCACTGTTT TTTCTCGAAC AGAACACTTA 
AGCCCTCTCA CCGTCATCAC CAAAGAGACT GAAAAAGCAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269070.1
CDS	229..3138
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001282141.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282141.2

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGGTGA GGTGGTCACT TATCAGTGGA ACATCCCAGA GAGGTCTGGC 
CCTGGGCCCA ATGACTCTGC TTGTGTTTCC TGGATCTATT ATTCTGCAGT GGATCCCATC 
AAGGACATGT ATAGTGGCCT GGTGGGGCCC TTGGCTATCT GCCAAAAGGG CATCCTGGAG 
CCCCATGGAG GACGGAGTGA CATGGATCGG GAATTTGCAT TGTTGTTCTT GATTTTTGAT 
GAAAATAAGT CTTGGTATTT GGAGGAAAAT GTGGCAACCC ATGGGTCCCA GGATCCAGGC 
AGTATTAACC TACAGGATGA AACTTTCTTG GAGAGCAATA AAATGCATGC AATCAATGGG 
AAACTCTATG CCAACCTTAG GGGTCTTACC ATGTACCAAG GAGAACGAGT GGCCTGGTAC 
ATGCTGGCCA TGGGCCAAGA TGTGGATCTA CACACCATCC ACTTTCATGC AGAGAGCTTC 
CTCTATCGGA ATGGCGAGAA CTACCGGGCA GATGTGGTGG ATCTGTTCCC AGGGACTTTT 
GAGGTTGTGG AGATGGTGGC CAGCAACCCT GGGACATGGC TGATGCACTG CCATGTGACT 
GACCATGTCC ATGCTGGCAT GGAGACCCTC TTCACTGTTT TTTCTCGAAC AGAACACTTA 
AGCCCTCTCA CCGTCATCAC CAAAGAGACT GAAAAAGCAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367243.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354172.1 Homo sapiens hephaestin (HEPH), transcript variant 14, mRNA.

Price & Availability↑

CloneID	OHu99554
Clone ID Related Accession (Same CDS sequence)	XM_017030003.1 , NM_001367243.1 , NM_001367243.2
Accession Version	NM_001367243.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	hephaestin isoform k precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Nov 14, 2018 this sequence version replaced XM_017030003.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.119629.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGCAAT CAATGGGAAA CTCTATGCCA ACCTTAGGGG TCTTACCATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG CTGGCCATGG GCCAAGATGT GGATCTACAC 
ACCATCCACT TTCATGCAGA GAGCTTCCTC TATCGGAATG GCGAGAACTA CCGGGCAGAT 
GTGGTGGATC TGTTCCCAGG GACTTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGA TGCACTGCCA TGTGACTGAC CATGTCCATG CTGGCATGGA GACCCTCTTC 
ACTGTTTTTT CTCGAACAGA ACACTTAAGC CCTCTCACCG TCATCACCAA AGAGACTGAA 
AAAGCAGTGC CCCCCAGAGA CATTGAAGAA GGCAATGTGA AGATGCTGGG CATGCAGATC 
CCCATAAAGA ATGTTGAGAT GCTGGCCTCT GTTTTGGTTG CCATTAGTGT CACCCTTCTG 
CTCGTTGTTC TGGCTCTTGG TGGAGTGGTT TGGTACCAAC ATCGACAGAG AAAGCTACGA 
CGCAATAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTTTCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354172.1
CDS	229..2805
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367243.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367243.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGCAAT CAATGGGAAA CTCTATGCCA ACCTTAGGGG TCTTACCATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG CTGGCCATGG GCCAAGATGT GGATCTACAC 
ACCATCCACT TTCATGCAGA GAGCTTCCTC TATCGGAATG GCGAGAACTA CCGGGCAGAT 
GTGGTGGATC TGTTCCCAGG GACTTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGA TGCACTGCCA TGTGACTGAC CATGTCCATG CTGGCATGGA GACCCTCTTC 
ACTGTTTTTT CTCGAACAGA ACACTTAAGC CCTCTCACCG TCATCACCAA AGAGACTGAA 
AAAGCAGTGC CCCCCAGAGA CATTGAAGAA GGCAATGTGA AGATGCTGGG CATGCAGATC 
CCCATAAAGA ATGTTGAGAT GCTGGCCTCT GTTTTGGTTG CCATTAGTGT CACCCTTCTG 
CTCGTTGTTC TGGCTCTTGG TGGAGTGGTT TGGTACCAAC ATCGACAGAG AAAGCTACGA 
CGCAATAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTTTCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367243.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354172.1 Homo sapiens hephaestin (HEPH), transcript variant 14, mRNA.

Price & Availability↑

CloneID	OHu99554
Clone ID Related Accession (Same CDS sequence)	XM_017030003.1 , NM_001367243.1 , NM_001367243.2
Accession Version	NM_001367243.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	hephaestin isoform k precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_001367243.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.119629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGCAAT CAATGGGAAA CTCTATGCCA ACCTTAGGGG TCTTACCATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG CTGGCCATGG GCCAAGATGT GGATCTACAC 
ACCATCCACT TTCATGCAGA GAGCTTCCTC TATCGGAATG GCGAGAACTA CCGGGCAGAT 
GTGGTGGATC TGTTCCCAGG GACTTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGA TGCACTGCCA TGTGACTGAC CATGTCCATG CTGGCATGGA GACCCTCTTC 
ACTGTTTTTT CTCGAACAGA ACACTTAAGC CCTCTCACCG TCATCACCAA AGAGACTGAA 
AAAGCAGTGC CCCCCAGAGA CATTGAAGAA GGCAATGTGA AGATGCTGGG CATGCAGATC 
CCCATAAAGA ATGTTGAGAT GCTGGCCTCT GTTTTGGTTG CCATTAGTGT CACCCTTCTG 
CTCGTTGTTC TGGCTCTTGG TGGAGTGGTT TGGTACCAAC ATCGACAGAG AAAGCTACGA 
CGCAATAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTTTCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354172.1
CDS	229..2805
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367243.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367243.2

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGCAAT CAATGGGAAA CTCTATGCCA ACCTTAGGGG TCTTACCATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG CTGGCCATGG GCCAAGATGT GGATCTACAC 
ACCATCCACT TTCATGCAGA GAGCTTCCTC TATCGGAATG GCGAGAACTA CCGGGCAGAT 
GTGGTGGATC TGTTCCCAGG GACTTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGA TGCACTGCCA TGTGACTGAC CATGTCCATG CTGGCATGGA GACCCTCTTC 
ACTGTTTTTT CTCGAACAGA ACACTTAAGC CCTCTCACCG TCATCACCAA AGAGACTGAA 
AAAGCAGTGC CCCCCAGAGA CATTGAAGAA GGCAATGTGA AGATGCTGGG CATGCAGATC 
CCCATAAAGA ATGTTGAGAT GCTGGCCTCT GTTTTGGTTG CCATTAGTGT CACCCTTCTG 
CTCGTTGTTC TGGCTCTTGG TGGAGTGGTT TGGTACCAAC ATCGACAGAG AAAGCTACGA 
CGCAATAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTTTCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_017030003.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885492.1 Homo sapiens hephaestin (HEPH), transcript variant X10, mRNA.

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CloneID	OHu99554
Clone ID Related Accession (Same CDS sequence)	XM_017030003.1 , NM_001367243.1 , NM_001367243.2
Accession Version	XM_017030003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGCAAT CAATGGGAAA CTCTATGCCA ACCTTAGGGG TCTTACCATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG CTGGCCATGG GCCAAGATGT GGATCTACAC 
ACCATCCACT TTCATGCAGA GAGCTTCCTC TATCGGAATG GCGAGAACTA CCGGGCAGAT 
GTGGTGGATC TGTTCCCAGG GACTTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGA TGCACTGCCA TGTGACTGAC CATGTCCATG CTGGCATGGA GACCCTCTTC 
ACTGTTTTTT CTCGAACAGA ACACTTAAGC CCTCTCACCG TCATCACCAA AGAGACTGAA 
AAAGCAGTGC CCCCCAGAGA CATTGAAGAA GGCAATGTGA AGATGCTGGG CATGCAGATC 
CCCATAAAGA ATGTTGAGAT GCTGGCCTCT GTTTTGGTTG CCATTAGTGT CACCCTTCTG 
CTCGTTGTTC TGGCTCTTGG TGGAGTGGTT TGGTACCAAC ATCGACAGAG AAAGCTACGA 
CGCAATAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTTTCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885492.1
CDS	103..2679
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_017030003.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030003.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GGACATTTGA GATTTATTGC CAGGCAGGCA GCCATCGAGA AGCAGGGATG 
AGGGCAATCT ATAATGTCTC CCAGTGTCCT GGCCACCAAG CCACCCCTCG CCAACGCTAC 
CAAGCTGCAA GAATCTACTA TATCATGGCA GAAGAAGTAG AGTGGGACTA TTGCCCTGAC 
CGGAGCTGGG AACGGGAATG GCACAACCAG TCTGAGAAGG ACAGTTATGG TTACATTTTC 
CTGAGCAACA AGGATGGGCT CCTGGGTTCC AGATACAAGA AAGCTGTATT CAGGGAATAC 
ACTGATGGTA CATTCAGGAT CCCTCGGCCA AGGACTGGAC CAGAAGAACA CTTGGGAATC 
TTGGGTCCAC TTATCAAAGG TGAAGTTGGT GATATCCTGA CTGTGGTATT CAAGAATAAT 
GCCAGCCGCC CCTACTCTGT GCATGCTCAT GGAGTGCTAG AATCTACTAC TGTCTGGCCA 
CTGGCTGCTG AGCCTGCAAT CAATGGGAAA CTCTATGCCA ACCTTAGGGG TCTTACCATG 
TACCAAGGAG AACGAGTGGC CTGGTACATG CTGGCCATGG GCCAAGATGT GGATCTACAC 
ACCATCCACT TTCATGCAGA GAGCTTCCTC TATCGGAATG GCGAGAACTA CCGGGCAGAT 
GTGGTGGATC TGTTCCCAGG GACTTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG 
ACATGGCTGA TGCACTGCCA TGTGACTGAC CATGTCCATG CTGGCATGGA GACCCTCTTC 
ACTGTTTTTT CTCGAACAGA ACACTTAAGC CCTCTCACCG TCATCACCAA AGAGACTGAA 
AAAGCAGTGC CCCCCAGAGA CATTGAAGAA GGCAATGTGA AGATGCTGGG CATGCAGATC 
CCCATAAAGA ATGTTGAGAT GCTGGCCTCT GTTTTGGTTG CCATTAGTGT CACCCTTCTG 
CTCGTTGTTC TGGCTCTTGG TGGAGTGGTT TGGTACCAAC ATCGACAGAG AAAGCTACGA 
CGCAATAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTTTCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367233.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354162.1 Homo sapiens hephaestin (HEPH), transcript variant 6, mRNA.

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CloneID	OHu54271
Clone ID Related Accession (Same CDS sequence)	NM_001367233.2 , XM_006724722.1 , NM_001367234.1 , NM_001367232.1 , NM_001367233.1 , NM_001367232.2
Accession Version	NM_001367233.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	hephaestin isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_001367233.1. Transcript Variant: This variant (6), as well as variants 5 and 7, encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.155480.1, SRR1803611.254440.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354162.1
CDS	229..3714
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367233.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367233.2

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367234.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354163.1 Homo sapiens hephaestin (HEPH), transcript variant 7, mRNA.

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CloneID	OHu54271
Clone ID Related Accession (Same CDS sequence)	NM_001367233.2 , XM_006724722.1 , NM_001367234.1 , NM_001367232.1 , NM_001367233.1 , NM_001367232.2
Accession Version	NM_001367234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	hephaestin isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. Transcript Variant: This variant (7), as well as variants 5 and 6, encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.114558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2156099 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354163.1
CDS	49..3534
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367234.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367234.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367232.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354161.1 Homo sapiens hephaestin (HEPH), transcript variant 5, mRNA.

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CloneID	OHu54271
Clone ID Related Accession (Same CDS sequence)	NM_001367233.2 , XM_006724722.1 , NM_001367234.1 , NM_001367232.1 , NM_001367233.1 , NM_001367232.2
Accession Version	NM_001367232.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	hephaestin isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Nov 14, 2018 this sequence version replaced XM_024452485.1. Transcript Variant: This variant (5), as well as variants 6 and 7, encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.150860.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354161.1
CDS	254..3739
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367232.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367232.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367233.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354162.1 Homo sapiens hephaestin (HEPH), transcript variant 6, mRNA.

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CloneID	OHu54271
Clone ID Related Accession (Same CDS sequence)	NM_001367233.2 , XM_006724722.1 , NM_001367234.1 , NM_001367232.1 , NM_001367233.1 , NM_001367232.2
Accession Version	NM_001367233.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	hephaestin isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Nov 14, 2018 this sequence version replaced XM_006724722.2. Transcript Variant: This variant (6), as well as variants 5 and 7, encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.156536.1, SRR1803615.1668.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354162.1
CDS	229..3714
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367233.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367233.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367232.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354161.1 Homo sapiens hephaestin (HEPH), transcript variant 5, mRNA.

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CloneID	OHu54271
Clone ID Related Accession (Same CDS sequence)	NM_001367233.2 , XM_006724722.1 , NM_001367234.1 , NM_001367232.1 , NM_001367233.1 , NM_001367232.2
Accession Version	NM_001367232.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-17
Organism	Homo sapiens(human)
Product	hephaestin isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_001367232.1. Transcript Variant: This variant (5), as well as variants 6 and 7, encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.150860.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354161.1
CDS	254..3739
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367232.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367232.2

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_006724722.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724785.1 Homo sapiens hephaestin (HEPH), transcript variant X2, mRNA.

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CloneID	OHu54271
Clone ID Related Accession (Same CDS sequence)	NM_001367233.2 , XM_006724722.1 , NM_001367234.1 , NM_001367232.1 , NM_001367233.1 , NM_001367232.2
Accession Version	XM_006724722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724785.1
CDS	178..3663
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_006724722.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724722.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367236.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354165.1 Homo sapiens hephaestin (HEPH), transcript variant 8, mRNA.

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CloneID	OHu107161
Clone ID Related Accession (Same CDS sequence)	NM_001367236.1 , NM_001367236.2
Accession Version	NM_001367236.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	hephaestin isoform f precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.221786.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGTG 
ATCCGGGCTG AGGTGGGTGA CACCATTCAG GTGGTCTTCT ACAACCGTGC CTCCCAGCCA 
TTCAGCATGC AGCCCCATGG GGTCTTTTAT GAGAAAGACT ATGAAGGCAC TGTGTACAAT 
GATGGCTCAT CTTACCCTGG CTTGGTTGCC AAGCCCTTTG AGAAAGTAAC ATACCGCTGG 
ACAGTCCCCC CTCATGCCGG TCCCACTGCT CAGGATCCTG CTTGTCTCAC TTGGATGTAC 
TTCTCTGCTG CAGATCCCAT AAGAGACACA AATTCTGGCC TGGTGGGCCC GCTGCTGGTG 
TGCAGGGCTG GTGCCTTGGG TGCAGATGGC AAGCAGAAAG GGGTGGATAA AGAATTCTTT 
CTTCTCTTCA CTGTGTTGGA TGAGAACAAG AGCTGGTACA GCAATGCCAA TCAAGCAGCT 
GCTATGTTGG ATTTCCGACT GCTTTCAGAG GATATTGAGG GCTTCCAAGA CTCCAATCGG 
ATGCATGCCA TTAATGGGTT TCTGTTCTCT AACCTGCCCA GGCTGGACAT GTGCAAGGGT 
GACACAGTGG CCTGGCACCT GCTCGGCCTG GGCACAGAGA CTGATGTGCA TGGAGTCATG 
TTCCAGGGCA ACACTGTGCA GCTTCAGGGC ATGAGGAAGG GTGCAGCTAT GCTCTTTCCT 
CATACCTTTG TCATGGCCAT CATGCAGCCT GACAACCTTG GGACATTTGA GATTTATTGC 
CAGGCAGGCA GCCATCGAGA AGCAGGGATG AGGGCAATCT ATAATGTCTC CCAGTGTCCT 
GGCCACCAAG CCACCCCTCG CCAACGCTAC CAAGCTGCAA GAATCTACTA TATCATGGCA 
GAAGAAGTAG AGTGGGACTA TTGCCCTGAC CGGAGCTGGG AACGGGAATG GCACAACCAG 
TCTGAGAAGG ACAGTTATGG TTACATTTTC CTGAGCAACA AGGATGGGCT CCTGGGTTCC 
AGATACAAGA AAGCTGTATT CAGGGAATAC ACTGATGGTA CATTCAGGAT CCCTCGGCCA 
AGGACTGGAC CAGAAGAACA CTTGGGAATC TTGGGTCCAC TTATCAAAGG TGAAGTTGGT 
GATATCCTGA CTGTGGTATT CAAGAATAAT GCCAGCCGCC CCTACTCTGT GCATGCTCAT 
GGAGTGCTAG AATCTACTAC TGTCTGGCCA CTGGCTGCTG AGCCTGGTGA GGTGGTCACT 
TATCAGTGGA ACATCCCAGA GAGGTCTGGC CCTGGGCCCA ATGACTCTGC TTGTGTTTCC 
TGGATCTATT ATTCTGCAGT GGATCCCATC AAGGACATGT ATAGTGGCCT GGTGGGGCCC 
TTGGCTATCT GCCAAAAGGG CATCCTGGAG CCCCATGGAG GACGGAGTGA CATGGATCGG 
GAATTTGCAT TGTTGTTCTT GATTTTTGAT GAAAATAAGT CTTGGTATTT GGAGGAAAAT 
GTGGCAACCC ATGGGTCCCA GGATCCAGGC AGTATTAACC TACAGGATGA AACTTTCTTG 
GAGAGCAATA AAATGCATGC AATCAATGGG AAACTCTATG CCAACCTTAG GGGTCTTACC 
ATGTACCAAG GAGAACGAGT GGCCTGGTAC ATGCTGGCCA TGGGCCAAGA TGTGGATCTA 
CACACCATCC ACTTTCATGC AGAGAGCTTC CTCTATCGGA ATGGCGAGAA CTACCGGGCA 
GATGTGGTGG ATCTGTTCCC AGGGACTTTT GAGGTTGTGG AGATGGTGGC CAGCAACCCT 
GGGACATGGC TGATGCACTG CCATGTGACT GACCATGTCC ATGCTGGCAT GGAGACCCTC 
TTCACTGTTT TTTCTCGAAC AGAACACTTA AGCCCTCTCA CCGTCATCAC CAAAGAGACT 
GAAAAAGCAG TGCCCCCCAG AGACATTGAA GAAGGCAATG TGAAGATGCT GGGCATGCAG 
ATCCCCATAA AGAATGTTGA GATGCTGGCC TCTGTTTTGG TTGCCATTAG TGTCACCCTT 
CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG GTTTGGTACC AACATCGACA GAGAAAGCTA 
CGACGCAATA GGAGGTCCAT CCTGGATGAC AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354165.1
CDS	229..3708
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367236.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367236.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGTG 
ATCCGGGCTG AGGTGGGTGA CACCATTCAG GTGGTCTTCT ACAACCGTGC CTCCCAGCCA 
TTCAGCATGC AGCCCCATGG GGTCTTTTAT GAGAAAGACT ATGAAGGCAC TGTGTACAAT 
GATGGCTCAT CTTACCCTGG CTTGGTTGCC AAGCCCTTTG AGAAAGTAAC ATACCGCTGG 
ACAGTCCCCC CTCATGCCGG TCCCACTGCT CAGGATCCTG CTTGTCTCAC TTGGATGTAC 
TTCTCTGCTG CAGATCCCAT AAGAGACACA AATTCTGGCC TGGTGGGCCC GCTGCTGGTG 
TGCAGGGCTG GTGCCTTGGG TGCAGATGGC AAGCAGAAAG GGGTGGATAA AGAATTCTTT 
CTTCTCTTCA CTGTGTTGGA TGAGAACAAG AGCTGGTACA GCAATGCCAA TCAAGCAGCT 
GCTATGTTGG ATTTCCGACT GCTTTCAGAG GATATTGAGG GCTTCCAAGA CTCCAATCGG 
ATGCATGCCA TTAATGGGTT TCTGTTCTCT AACCTGCCCA GGCTGGACAT GTGCAAGGGT 
GACACAGTGG CCTGGCACCT GCTCGGCCTG GGCACAGAGA CTGATGTGCA TGGAGTCATG 
TTCCAGGGCA ACACTGTGCA GCTTCAGGGC ATGAGGAAGG GTGCAGCTAT GCTCTTTCCT 
CATACCTTTG TCATGGCCAT CATGCAGCCT GACAACCTTG GGACATTTGA GATTTATTGC 
CAGGCAGGCA GCCATCGAGA AGCAGGGATG AGGGCAATCT ATAATGTCTC CCAGTGTCCT 
GGCCACCAAG CCACCCCTCG CCAACGCTAC CAAGCTGCAA GAATCTACTA TATCATGGCA 
GAAGAAGTAG AGTGGGACTA TTGCCCTGAC CGGAGCTGGG AACGGGAATG GCACAACCAG 
TCTGAGAAGG ACAGTTATGG TTACATTTTC CTGAGCAACA AGGATGGGCT CCTGGGTTCC 
AGATACAAGA AAGCTGTATT CAGGGAATAC ACTGATGGTA CATTCAGGAT CCCTCGGCCA 
AGGACTGGAC CAGAAGAACA CTTGGGAATC TTGGGTCCAC TTATCAAAGG TGAAGTTGGT 
GATATCCTGA CTGTGGTATT CAAGAATAAT GCCAGCCGCC CCTACTCTGT GCATGCTCAT 
GGAGTGCTAG AATCTACTAC TGTCTGGCCA CTGGCTGCTG AGCCTGGTGA GGTGGTCACT 
TATCAGTGGA ACATCCCAGA GAGGTCTGGC CCTGGGCCCA ATGACTCTGC TTGTGTTTCC 
TGGATCTATT ATTCTGCAGT GGATCCCATC AAGGACATGT ATAGTGGCCT GGTGGGGCCC 
TTGGCTATCT GCCAAAAGGG CATCCTGGAG CCCCATGGAG GACGGAGTGA CATGGATCGG 
GAATTTGCAT TGTTGTTCTT GATTTTTGAT GAAAATAAGT CTTGGTATTT GGAGGAAAAT 
GTGGCAACCC ATGGGTCCCA GGATCCAGGC AGTATTAACC TACAGGATGA AACTTTCTTG 
GAGAGCAATA AAATGCATGC AATCAATGGG AAACTCTATG CCAACCTTAG GGGTCTTACC 
ATGTACCAAG GAGAACGAGT GGCCTGGTAC ATGCTGGCCA TGGGCCAAGA TGTGGATCTA 
CACACCATCC ACTTTCATGC AGAGAGCTTC CTCTATCGGA ATGGCGAGAA CTACCGGGCA 
GATGTGGTGG ATCTGTTCCC AGGGACTTTT GAGGTTGTGG AGATGGTGGC CAGCAACCCT 
GGGACATGGC TGATGCACTG CCATGTGACT GACCATGTCC ATGCTGGCAT GGAGACCCTC 
TTCACTGTTT TTTCTCGAAC AGAACACTTA AGCCCTCTCA CCGTCATCAC CAAAGAGACT 
GAAAAAGCAG TGCCCCCCAG AGACATTGAA GAAGGCAATG TGAAGATGCT GGGCATGCAG 
ATCCCCATAA AGAATGTTGA GATGCTGGCC TCTGTTTTGG TTGCCATTAG TGTCACCCTT 
CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG GTTTGGTACC AACATCGACA GAGAAAGCTA 
CGACGCAATA GGAGGTCCAT CCTGGATGAC AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367236.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354165.1 Homo sapiens hephaestin (HEPH), transcript variant 8, mRNA.

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CloneID	OHu107161
Clone ID Related Accession (Same CDS sequence)	NM_001367236.1 , NM_001367236.2
Accession Version	NM_001367236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	hephaestin isoform f precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_001367236.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.221786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2156099 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGTG 
ATCCGGGCTG AGGTGGGTGA CACCATTCAG GTGGTCTTCT ACAACCGTGC CTCCCAGCCA 
TTCAGCATGC AGCCCCATGG GGTCTTTTAT GAGAAAGACT ATGAAGGCAC TGTGTACAAT 
GATGGCTCAT CTTACCCTGG CTTGGTTGCC AAGCCCTTTG AGAAAGTAAC ATACCGCTGG 
ACAGTCCCCC CTCATGCCGG TCCCACTGCT CAGGATCCTG CTTGTCTCAC TTGGATGTAC 
TTCTCTGCTG CAGATCCCAT AAGAGACACA AATTCTGGCC TGGTGGGCCC GCTGCTGGTG 
TGCAGGGCTG GTGCCTTGGG TGCAGATGGC AAGCAGAAAG GGGTGGATAA AGAATTCTTT 
CTTCTCTTCA CTGTGTTGGA TGAGAACAAG AGCTGGTACA GCAATGCCAA TCAAGCAGCT 
GCTATGTTGG ATTTCCGACT GCTTTCAGAG GATATTGAGG GCTTCCAAGA CTCCAATCGG 
ATGCATGCCA TTAATGGGTT TCTGTTCTCT AACCTGCCCA GGCTGGACAT GTGCAAGGGT 
GACACAGTGG CCTGGCACCT GCTCGGCCTG GGCACAGAGA CTGATGTGCA TGGAGTCATG 
TTCCAGGGCA ACACTGTGCA GCTTCAGGGC ATGAGGAAGG GTGCAGCTAT GCTCTTTCCT 
CATACCTTTG TCATGGCCAT CATGCAGCCT GACAACCTTG GGACATTTGA GATTTATTGC 
CAGGCAGGCA GCCATCGAGA AGCAGGGATG AGGGCAATCT ATAATGTCTC CCAGTGTCCT 
GGCCACCAAG CCACCCCTCG CCAACGCTAC CAAGCTGCAA GAATCTACTA TATCATGGCA 
GAAGAAGTAG AGTGGGACTA TTGCCCTGAC CGGAGCTGGG AACGGGAATG GCACAACCAG 
TCTGAGAAGG ACAGTTATGG TTACATTTTC CTGAGCAACA AGGATGGGCT CCTGGGTTCC 
AGATACAAGA AAGCTGTATT CAGGGAATAC ACTGATGGTA CATTCAGGAT CCCTCGGCCA 
AGGACTGGAC CAGAAGAACA CTTGGGAATC TTGGGTCCAC TTATCAAAGG TGAAGTTGGT 
GATATCCTGA CTGTGGTATT CAAGAATAAT GCCAGCCGCC CCTACTCTGT GCATGCTCAT 
GGAGTGCTAG AATCTACTAC TGTCTGGCCA CTGGCTGCTG AGCCTGGTGA GGTGGTCACT 
TATCAGTGGA ACATCCCAGA GAGGTCTGGC CCTGGGCCCA ATGACTCTGC TTGTGTTTCC 
TGGATCTATT ATTCTGCAGT GGATCCCATC AAGGACATGT ATAGTGGCCT GGTGGGGCCC 
TTGGCTATCT GCCAAAAGGG CATCCTGGAG CCCCATGGAG GACGGAGTGA CATGGATCGG 
GAATTTGCAT TGTTGTTCTT GATTTTTGAT GAAAATAAGT CTTGGTATTT GGAGGAAAAT 
GTGGCAACCC ATGGGTCCCA GGATCCAGGC AGTATTAACC TACAGGATGA AACTTTCTTG 
GAGAGCAATA AAATGCATGC AATCAATGGG AAACTCTATG CCAACCTTAG GGGTCTTACC 
ATGTACCAAG GAGAACGAGT GGCCTGGTAC ATGCTGGCCA TGGGCCAAGA TGTGGATCTA 
CACACCATCC ACTTTCATGC AGAGAGCTTC CTCTATCGGA ATGGCGAGAA CTACCGGGCA 
GATGTGGTGG ATCTGTTCCC AGGGACTTTT GAGGTTGTGG AGATGGTGGC CAGCAACCCT 
GGGACATGGC TGATGCACTG CCATGTGACT GACCATGTCC ATGCTGGCAT GGAGACCCTC 
TTCACTGTTT TTTCTCGAAC AGAACACTTA AGCCCTCTCA CCGTCATCAC CAAAGAGACT 
GAAAAAGCAG TGCCCCCCAG AGACATTGAA GAAGGCAATG TGAAGATGCT GGGCATGCAG 
ATCCCCATAA AGAATGTTGA GATGCTGGCC TCTGTTTTGG TTGCCATTAG TGTCACCCTT 
CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG GTTTGGTACC AACATCGACA GAGAAAGCTA 
CGACGCAATA GGAGGTCCAT CCTGGATGAC AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354165.1
CDS	229..3708
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367236.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367236.2

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGTG 
ATCCGGGCTG AGGTGGGTGA CACCATTCAG GTGGTCTTCT ACAACCGTGC CTCCCAGCCA 
TTCAGCATGC AGCCCCATGG GGTCTTTTAT GAGAAAGACT ATGAAGGCAC TGTGTACAAT 
GATGGCTCAT CTTACCCTGG CTTGGTTGCC AAGCCCTTTG AGAAAGTAAC ATACCGCTGG 
ACAGTCCCCC CTCATGCCGG TCCCACTGCT CAGGATCCTG CTTGTCTCAC TTGGATGTAC 
TTCTCTGCTG CAGATCCCAT AAGAGACACA AATTCTGGCC TGGTGGGCCC GCTGCTGGTG 
TGCAGGGCTG GTGCCTTGGG TGCAGATGGC AAGCAGAAAG GGGTGGATAA AGAATTCTTT 
CTTCTCTTCA CTGTGTTGGA TGAGAACAAG AGCTGGTACA GCAATGCCAA TCAAGCAGCT 
GCTATGTTGG ATTTCCGACT GCTTTCAGAG GATATTGAGG GCTTCCAAGA CTCCAATCGG 
ATGCATGCCA TTAATGGGTT TCTGTTCTCT AACCTGCCCA GGCTGGACAT GTGCAAGGGT 
GACACAGTGG CCTGGCACCT GCTCGGCCTG GGCACAGAGA CTGATGTGCA TGGAGTCATG 
TTCCAGGGCA ACACTGTGCA GCTTCAGGGC ATGAGGAAGG GTGCAGCTAT GCTCTTTCCT 
CATACCTTTG TCATGGCCAT CATGCAGCCT GACAACCTTG GGACATTTGA GATTTATTGC 
CAGGCAGGCA GCCATCGAGA AGCAGGGATG AGGGCAATCT ATAATGTCTC CCAGTGTCCT 
GGCCACCAAG CCACCCCTCG CCAACGCTAC CAAGCTGCAA GAATCTACTA TATCATGGCA 
GAAGAAGTAG AGTGGGACTA TTGCCCTGAC CGGAGCTGGG AACGGGAATG GCACAACCAG 
TCTGAGAAGG ACAGTTATGG TTACATTTTC CTGAGCAACA AGGATGGGCT CCTGGGTTCC 
AGATACAAGA AAGCTGTATT CAGGGAATAC ACTGATGGTA CATTCAGGAT CCCTCGGCCA 
AGGACTGGAC CAGAAGAACA CTTGGGAATC TTGGGTCCAC TTATCAAAGG TGAAGTTGGT 
GATATCCTGA CTGTGGTATT CAAGAATAAT GCCAGCCGCC CCTACTCTGT GCATGCTCAT 
GGAGTGCTAG AATCTACTAC TGTCTGGCCA CTGGCTGCTG AGCCTGGTGA GGTGGTCACT 
TATCAGTGGA ACATCCCAGA GAGGTCTGGC CCTGGGCCCA ATGACTCTGC TTGTGTTTCC 
TGGATCTATT ATTCTGCAGT GGATCCCATC AAGGACATGT ATAGTGGCCT GGTGGGGCCC 
TTGGCTATCT GCCAAAAGGG CATCCTGGAG CCCCATGGAG GACGGAGTGA CATGGATCGG 
GAATTTGCAT TGTTGTTCTT GATTTTTGAT GAAAATAAGT CTTGGTATTT GGAGGAAAAT 
GTGGCAACCC ATGGGTCCCA GGATCCAGGC AGTATTAACC TACAGGATGA AACTTTCTTG 
GAGAGCAATA AAATGCATGC AATCAATGGG AAACTCTATG CCAACCTTAG GGGTCTTACC 
ATGTACCAAG GAGAACGAGT GGCCTGGTAC ATGCTGGCCA TGGGCCAAGA TGTGGATCTA 
CACACCATCC ACTTTCATGC AGAGAGCTTC CTCTATCGGA ATGGCGAGAA CTACCGGGCA 
GATGTGGTGG ATCTGTTCCC AGGGACTTTT GAGGTTGTGG AGATGGTGGC CAGCAACCCT 
GGGACATGGC TGATGCACTG CCATGTGACT GACCATGTCC ATGCTGGCAT GGAGACCCTC 
TTCACTGTTT TTTCTCGAAC AGAACACTTA AGCCCTCTCA CCGTCATCAC CAAAGAGACT 
GAAAAAGCAG TGCCCCCCAG AGACATTGAA GAAGGCAATG TGAAGATGCT GGGCATGCAG 
ATCCCCATAA AGAATGTTGA GATGCTGGCC TCTGTTTTGG TTGCCATTAG TGTCACCCTT 
CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG GTTTGGTACC AACATCGACA GAGAAAGCTA 
CGACGCAATA GGAGGTCCAT CCTGGATGAC AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367238.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354167.1 Homo sapiens hephaestin (HEPH), transcript variant 9, mRNA.

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CloneID	OHu99550
Clone ID Related Accession (Same CDS sequence)	XM_017029999.1 , NM_001367238.1 , NM_001367238.2
Accession Version	NM_001367238.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	hephaestin isoform g precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Nov 14, 2018 this sequence version replaced XM_017029999.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.103201.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354167.1
CDS	229..3501
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367238.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367238.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367238.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354167.1 Homo sapiens hephaestin (HEPH), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu99550
Clone ID Related Accession (Same CDS sequence)	XM_017029999.1 , NM_001367238.1 , NM_001367238.2
Accession Version	NM_001367238.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	hephaestin isoform g precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_001367238.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.103201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354167.1
CDS	229..3501
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367238.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367238.2

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367239.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354168.1 Homo sapiens hephaestin (HEPH), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu99551
Clone ID Related Accession (Same CDS sequence)	NM_001367239.1 , XM_017030000.1
Accession Version	NM_001367239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	hephaestin isoform h precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Nov 14, 2018 this sequence version replaced XM_017030000.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.134561.1, SRR1803617.110190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354168.1
CDS	229..3498
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367239.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367240.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354169.1 Homo sapiens hephaestin (HEPH), transcript variant 11, mRNA.

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CloneID	OHu99552
Clone ID Related Accession (Same CDS sequence)	XM_017030001.1 , NM_001367240.2 , NM_001367240.1
Accession Version	NM_001367240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	hephaestin isoform i precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Jun 2, 2019 this sequence version replaced NM_001367240.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.120473.1, SRR1660803.73793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354169.1
CDS	229..3381
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367240.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367240.2

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367240.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354169.1 Homo sapiens hephaestin (HEPH), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu99552
Clone ID Related Accession (Same CDS sequence)	XM_017030001.1 , NM_001367240.2 , NM_001367240.1
Accession Version	NM_001367240.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	hephaestin isoform i precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Nov 14, 2018 this sequence version replaced XM_017030001.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.73793.1, SRR1803614.83979.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354169.1
CDS	229..3381
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367240.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367240.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001367241.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354170.1 Homo sapiens hephaestin (HEPH), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu99553
Clone ID Related Accession (Same CDS sequence)	XM_017030002.1 , NM_001367241.1
Accession Version	NM_001367241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	hephaestin isoform j precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, KF459144.1 and AL157698.8. On Nov 14, 2018 this sequence version replaced XM_017030002.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.262012.1, SRR1660809.252280.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354170.1
CDS	229..3378
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001367241.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367241.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_017029998.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885487.1 Homo sapiens hephaestin (HEPH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu99549
Clone ID Related Accession (Same CDS sequence)	XM_017029998.1 , XM_017029998.2
Accession Version	XM_017029998.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGTGCCCCC CAGAGACATT GAAGAAGGCA ATGTGAAGAT GCTGGGCATG 
CAGATCCCCA TAAAGAATGT TGAGATGCTG GCCTCTGTTT TGGTTGCCAT TAGTGTCACC 
CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA GTGGTTTGGT ACCAACATCG ACAGAGAAAG 
CTACGACGCA ATAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TTTCAAACAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885487.1
CDS	203..3505
Translation	MTQTLPYHLSLLNVLFPGPCSRHFKFRRGKCAQPAWRKVSAPSQDLLITKVMWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_017029998.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029998.1

ORF Insert Sequence:

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGTGCCCCC CAGAGACATT GAAGAAGGCA ATGTGAAGAT GCTGGGCATG 
CAGATCCCCA TAAAGAATGT TGAGATGCTG GCCTCTGTTT TGGTTGCCAT TAGTGTCACC 
CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA GTGGTTTGGT ACCAACATCG ACAGAGAAAG 
CTACGACGCA ATAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TTTCAAACAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_017029998.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885487.1 Homo sapiens hephaestin (HEPH), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu99549
Clone ID Related Accession (Same CDS sequence)	XM_017029998.1 , XM_017029998.2
Accession Version	XM_017029998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGTGCCCCC CAGAGACATT GAAGAAGGCA ATGTGAAGAT GCTGGGCATG 
CAGATCCCCA TAAAGAATGT TGAGATGCTG GCCTCTGTTT TGGTTGCCAT TAGTGTCACC 
CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA GTGGTTTGGT ACCAACATCG ACAGAGAAAG 
CTACGACGCA ATAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TTTCAAACAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885487.1
CDS	203..3505
Translation	MTQTLPYHLSLLNVLFPGPCSRHFKFRRGKCAQPAWRKVSAPSQDLLITKVMWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_017029998.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029998.2

ORF Insert Sequence:

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGTGCCCCC CAGAGACATT GAAGAAGGCA ATGTGAAGAT GCTGGGCATG 
CAGATCCCCA TAAAGAATGT TGAGATGCTG GCCTCTGTTT TGGTTGCCAT TAGTGTCACC 
CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA GTGGTTTGGT ACCAACATCG ACAGAGAAAG 
CTACGACGCA ATAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TTTCAAACAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_017029999.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885488.1 Homo sapiens hephaestin (HEPH), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu99550
Clone ID Related Accession (Same CDS sequence)	XM_017029999.1 , NM_001367238.1 , NM_001367238.2
Accession Version	XM_017029999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885488.1
CDS	103..3375
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_017029999.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029999.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_017030000.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885489.1 Homo sapiens hephaestin (HEPH), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu99551
Clone ID Related Accession (Same CDS sequence)	NM_001367239.1 , XM_017030000.1
Accession Version	XM_017030000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885489.1
CDS	103..3372
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_017030000.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030000.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGGGACATT TGAGATTTAT TGCCAGGCAG GCAGCCATCG AGAAGCAGGG 
ATGAGGGCAA TCTATAATGT CTCCCAGTGT CCTGGCCACC AAGCCACCCC TCGCCAACGC 
TACCAAGCTG CAAGAATCTA CTATATCATG GCAGAAGAAG TAGAGTGGGA CTATTGCCCT 
GACCGGAGCT GGGAACGGGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTTACATT 
TTCCTGAGCA ACAAGGATGG GCTCCTGGGT TCCAGATACA AGAAAGCTGT ATTCAGGGAA 
TACACTGATG GTACATTCAG GATCCCTCGG CCAAGGACTG GACCAGAAGA ACACTTGGGA 
ATCTTGGGTC CACTTATCAA AGGTGAAGTT GGTGATATCC TGACTGTGGT ATTCAAGAAT 
AATGCCAGCC GCCCCTACTC TGTGCATGCT CATGGAGTGC TAGAATCTAC TACTGTCTGG 
CCACTGGCTG CTGAGCCTGG TGAGGTGGTC ACTTATCAGT GGAACATCCC AGAGAGGTCT 
GGCCCTGGGC CCAATGACTC TGCTTGTGTT TCCTGGATCT ATTATTCTGC AGTGGATCCC 
ATCAAGGACA TGTATAGTGG CCTGGTGGGG CCCTTGGCTA TCTGCCAAAA GGGCATCCTG 
GAGCCCCATG GAGGACGGAG TGACATGGAT CGGGAATTTG CATTGTTGTT CTTGATTTTT 
GATGAAAATA AGTCTTGGTA TTTGGAGGAA AATGTGGCAA CCCATGGGTC CCAGGATCCA 
GGCAGTATTA ACCTACAGGA TGAAACTTTC TTGGAGAGCA ATAAAATGCA TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_017030001.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885490.1 Homo sapiens hephaestin (HEPH), transcript variant X8, mRNA.

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CloneID	OHu99552
Clone ID Related Accession (Same CDS sequence)	XM_017030001.1 , NM_001367240.2 , NM_001367240.1
Accession Version	XM_017030001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885490.1
CDS	103..3255
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_017030001.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030001.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG CAGTGCCCCC CAGAGACATT 
GAAGAAGGCA ATGTGAAGAT GCTGGGCATG CAGATCCCCA TAAAGAATGT TGAGATGCTG 
GCCTCTGTTT TGGTTGCCAT TAGTGTCACC CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA 
GTGGTTTGGT ACCAACATCG ACAGAGAAAG CTACGACGCA ATAGGAGGTC CATCCTGGAT 
GACAGCTTCA AGCTTCTGTC TTTCAAACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_017030002.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885491.1 Homo sapiens hephaestin (HEPH), transcript variant X9, mRNA.

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CloneID	OHu99553
Clone ID Related Accession (Same CDS sequence)	XM_017030002.1 , NM_001367241.1
Accession Version	XM_017030002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885491.1
CDS	103..3252
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_017030002.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030002.1

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGCAATCAAT 
GGGAAACTCT ATGCCAACCT TAGGGGTCTT ACCATGTACC AAGGAGAACG AGTGGCCTGG 
TACATGCTGG CCATGGGCCA AGATGTGGAT CTACACACCA TCCACTTTCA TGCAGAGAGC 
TTCCTCTATC GGAATGGCGA GAACTACCGG GCAGATGTGG TGGATCTGTT CCCAGGGACT 
TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGATGCA CTGCCATGTG 
ACTGACCATG TCCATGCTGG CATGGAGACC CTCTTCACTG TTTTTTCTCG AACAGAACAC 
TTAAGCCCTC TCACCGTCAT CACCAAAGAG ACTGAAAAAG TGCCCCCCAG AGACATTGAA 
GAAGGCAATG TGAAGATGCT GGGCATGCAG ATCCCCATAA AGAATGTTGA GATGCTGGCC 
TCTGTTTTGG TTGCCATTAG TGTCACCCTT CTGCTCGTTG TTCTGGCTCT TGGTGGAGTG 
GTTTGGTACC AACATCGACA GAGAAAGCTA CGACGCAATA GGAGGTCCAT CCTGGATGAC 
AGCTTCAAGC TTCTGTCTTT CAAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001130860.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124332.1 Homo sapiens hephaestin (HEPH), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11051
Clone ID Related Accession (Same CDS sequence)	NM_001130860.4 , NM_001130860.3
Accession Version	NM_001130860.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	hephaestin isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN361718.1, AY358990.1 and BC011561.1. On Jun 2, 2019 this sequence version replaced NM_001130860.3. Transcript Variant: This variant (3) contains an alternate 5'-most exon, uses an alternate splice site in the 3' coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (c) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358990.1, SRR1803615.226168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGTGCCCCC CAGAGACATT GAAGAAGGCA ATGTGAAGAT GCTGGGCATG 
CAGATCCCCA TAAAGAATGT TGAGATGCTG GCCTCTGTTT TGGTTGCCAT TAGTGTCACC 
CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA GTGGTTTGGT ACCAACATCG ACAGAGAAAG 
CTACGACGCA ATAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TTTCAAACAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124332.1
CDS	229..3711
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001130860.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130860.4

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGTGCCCCC CAGAGACATT GAAGAAGGCA ATGTGAAGAT GCTGGGCATG 
CAGATCCCCA TAAAGAATGT TGAGATGCTG GCCTCTGTTT TGGTTGCCAT TAGTGTCACC 
CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA GTGGTTTGGT ACCAACATCG ACAGAGAAAG 
CTACGACGCA ATAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TTTCAAACAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_001130860.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124332.1 Homo sapiens hephaestin (HEPH), transcript variant 3, mRNA.

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CloneID	OHu11051
Clone ID Related Accession (Same CDS sequence)	NM_001130860.4 , NM_001130860.3
Accession Version	NM_001130860.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	hephaestin isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL030998.1, CN361718.1, AY358990.1 and BC011561.1. On Aug 20, 2013 this sequence version replaced NM_001130860.2. Transcript Variant: This variant (3) contains an alternate 5'-most exon, uses an alternate splice site in the 3' coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (c) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358990.1, SRR1803611.150330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGTGCCCCC CAGAGACATT GAAGAAGGCA ATGTGAAGAT GCTGGGCATG 
CAGATCCCCA TAAAGAATGT TGAGATGCTG GCCTCTGTTT TGGTTGCCAT TAGTGTCACC 
CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA GTGGTTTGGT ACCAACATCG ACAGAGAAAG 
CTACGACGCA ATAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TTTCAAACAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124332.1
CDS	246..3728
Translation	MWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_001130860.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130860.3

ORF Insert Sequence:

ATGTGGGCCA TGGAGTCAGG CCACCTCCTC TGGGCTCTGC TGTTCATGCA GTCCTTGTGG 
CCTCAACTGA CTGATGGAGC CACTCGAGTC TACTACCTGG GCATCCGGGA TGTGCAGTGG 
AACTATGCTC CCAAGGGAAG AAATGTCATC ACGAACCAGC CTCTGGACAG TGACATAGTG 
GCTTCCAGCT TCTTAAAGTC TGACAAGAAC CGGATAGGGG GAACCTACAA GAAGACCATC 
TATAAAGAAT ACAAGGATGA CTCATACACA GATGAAGTGG CCCAGCCTGC CTGGTTGGGC 
TTCCTGGGGC CAGTGTTGCA GGCTGAAGTG GGGGATGTCA TTCTTATTCA CCTGAAGAAT 
TTTGCCACTC GTCCCTATAC CATCCACCCT CATGGTGTCT TCTACGAGAA GGACTCTGAA 
GGTTCCCTAT ACCCAGATGG CTCCTCTGGG CCACTGAAAG CTGATGACTC TGTTCCCCCG 
GGGGGCAGCC ATATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC CGATGCTGAC 
CCAGCGTGCC TCACCTGGAT CTACCATTCT CATGTAGATG CTCCACGAGA CATTGCAACT 
GGCCTAATTG GGCCTCTCAT CACCTGTAAA AGAGGAGCCC TGGATGGGAA CTCCCCTCCT 
CAACGCCAGG ATGTAGACCA TGATTTCTTC CTCCTCTTCA GTGTGGTAGA TGAGAACCTC 
AGCTGGCATC TCAATGAGAA CATTGCCACT TACTGCTCAG ATCCTGCTTC AGTGGACAAA 
GAAGATGAGA CATTTCAGGA GAGCAATAGG ATGCATGCAA TCAATGGCTT TGTTTTTGGG 
AATTTACCTG AGCTGAACAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG 
GGCAATGAAA TTGATGTCCA CACAGCATTT TTCCATGGAC AGATGCTGAC TACCCGTGGA 
CACCACACTG ATGTGGCTAA CATCTTTCCA GCCACCTTTG TGACTGCTGA GATGGTGCCC 
TGGGAACCTG GTACCTGGTT AATTAGCTGC CAAGTGAACA GTCACTTTCG AGATGGCATG 
CAGGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCCCCTC CTGTGGACCT GCTCACAGGC 
AAAGTTCGAC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCGATGGGG 
CATGATGGGA GTACTGGGAA GAATTTGAGA GAGCCAGGCA GTATCTCAGA TAAGTTTTTC 
CAGAAGAGCT CCAGCCGAAT TGGGGGCACT TACTGGAAAG TGCGATATGA AGCCTTTCAA 
GATGAGACAT TCCAAGAGAA GATGCATTTG GAGGAAGATA GGCATCTTGG AATCCTGGGG 
CCAGTGATCC GGGCTGAGGT GGGTGACACC ATTCAGGTGG TCTTCTACAA CCGTGCCTCC 
CAGCCATTCA GCATGCAGCC CCATGGGGTC TTTTATGAGA AAGACTATGA AGGCACTGTG 
TACAATGATG GCTCATCTTA CCCTGGCTTG GTTGCCAAGC CCTTTGAGAA AGTAACATAC 
CGCTGGACAG TCCCCCCTCA TGCCGGTCCC ACTGCTCAGG ATCCTGCTTG TCTCACTTGG 
ATGTACTTCT CTGCTGCAGA TCCCATAAGA GACACAAATT CTGGCCTGGT GGGCCCGCTG 
CTGGTGTGCA GGGCTGGTGC CTTGGGTGCA GATGGCAAGC AGAAAGGGGT GGATAAAGAA 
TTCTTTCTTC TCTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA 
GCAGCTGCTA TGTTGGATTT CCGACTGCTT TCAGAGGATA TTGAGGGCTT CCAAGACTCC 
AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAACC TGCCCAGGCT GGACATGTGC 
AAGGGTGACA CAGTGGCCTG GCACCTGCTC GGCCTGGGCA CAGAGACTGA TGTGCATGGA 
GTCATGTTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGGGTGC AGCTATGCTC 
TTTCCTCATA CCTTTGTCAT GGCCATCATG CAGCCTGACA ACCTTGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGTGCCCCC CAGAGACATT GAAGAAGGCA ATGTGAAGAT GCTGGGCATG 
CAGATCCCCA TAAAGAATGT TGAGATGCTG GCCTCTGTTT TGGTTGCCAT TAGTGTCACC 
CTTCTGCTCG TTGTTCTGGC TCTTGGTGGA GTGGTTTGGT ACCAACATCG ACAGAGAAAG 
CTACGACGCA ATAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TTTCAAACAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( NM_138737.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_620074.3 Homo sapiens hephaestin (HEPH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12228
Clone ID Related Accession (Same CDS sequence)	NM_138737.4 , NM_138737.5
Accession Version	NM_138737.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	hephaestin isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP282214.1, AL030998.1 and BC011561.1. On Aug 20, 2013 this sequence version replaced NM_138737.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGGTGAG GTGGTCACTT ATCAGTGGAA CATCCCAGAG 
AGGTCTGGCC CTGGGCCCAA TGACTCTGCT TGTGTTTCCT GGATCTATTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA TAGTGGCCTG GTGGGGCCCT TGGCTATCTG CCAAAAGGGC 
ATCCTGGAGC CCCATGGAGG ACGGAGTGAC ATGGATCGGG AATTTGCATT GTTGTTCTTG 
ATTTTTGATG AAAATAAGTC TTGGTATTTG GAGGAAAATG TGGCAACCCA TGGGTCCCAG 
GATCCAGGCA GTATTAACCT ACAGGATGAA ACTTTCTTGG AGAGCAATAA AATGCATGCA 
ATCAATGGGA AACTCTATGC CAACCTTAGG GGTCTTACCA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGCTGGCCAT GGGCCAAGAT GTGGATCTAC ACACCATCCA CTTTCATGCA 
GAGAGCTTCC TCTATCGGAA TGGCGAGAAC TACCGGGCAG ATGTGGTGGA TCTGTTCCCA 
GGGACTTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GATGCACTGC 
CATGTGACTG ACCATGTCCA TGCTGGCATG GAGACCCTCT TCACTGTTTT TTCTCGAACA 
GAACACTTAA GCCCTCTCAC CGTCATCACC AAAGAGACTG AAAAAGCAGT GCCCCCCAGA 
GACATTGAAG AAGGCAATGT GAAGATGCTG GGCATGCAGA TCCCCATAAA GAATGTTGAG 
ATGCTGGCCT CTGTTTTGGT TGCCATTAGT GTCACCCTTC TGCTCGTTGT TCTGGCTCTT 
GGTGGAGTGG TTTGGTACCA ACATCGACAG AGAAAGCTAC GACGCAATAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTTTC AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620074.3
CDS	180..3818
Translation	MTQTLPYHLSLLNVLFPGPCSRHFKFRRGKCAQPAWRKVSAPSQDLLITKVMWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	NM_138737.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138737.4

ORF Insert Sequence:

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGGTGAG GTGGTCACTT ATCAGTGGAA CATCCCAGAG 
AGGTCTGGCC CTGGGCCCAA TGACTCTGCT TGTGTTTCCT GGATCTATTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA TAGTGGCCTG GTGGGGCCCT TGGCTATCTG CCAAAAGGGC 
ATCCTGGAGC CCCATGGAGG ACGGAGTGAC ATGGATCGGG AATTTGCATT GTTGTTCTTG 
ATTTTTGATG AAAATAAGTC TTGGTATTTG GAGGAAAATG TGGCAACCCA TGGGTCCCAG 
GATCCAGGCA GTATTAACCT ACAGGATGAA ACTTTCTTGG AGAGCAATAA AATGCATGCA 
ATCAATGGGA AACTCTATGC CAACCTTAGG GGTCTTACCA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGCTGGCCAT GGGCCAAGAT GTGGATCTAC ACACCATCCA CTTTCATGCA 
GAGAGCTTCC TCTATCGGAA TGGCGAGAAC TACCGGGCAG ATGTGGTGGA TCTGTTCCCA 
GGGACTTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GATGCACTGC 
CATGTGACTG ACCATGTCCA TGCTGGCATG GAGACCCTCT TCACTGTTTT TTCTCGAACA 
GAACACTTAA GCCCTCTCAC CGTCATCACC AAAGAGACTG AAAAAGCAGT GCCCCCCAGA 
GACATTGAAG AAGGCAATGT GAAGATGCTG GGCATGCAGA TCCCCATAAA GAATGTTGAG 
ATGCTGGCCT CTGTTTTGGT TGCCATTAGT GTCACCCTTC TGCTCGTTGT TCTGGCTCTT 
GGTGGAGTGG TTTGGTACCA ACATCGACAG AGAAAGCTAC GACGCAATAG GAGGTCCATC 
CTGGATGACA GCTTCAAGCT TCTGTCTTTC AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_011531073.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529375.1 Homo sapiens hephaestin (HEPH), transcript variant X1, mRNA.

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CloneID	OHu63368
Clone ID Related Accession (Same CDS sequence)	XM_011531073.2 , XM_011531073.3
Accession Version	XM_011531073.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGGTGAG GTGGTCACTT ATCAGTGGAA CATCCCAGAG 
AGGTCTGGCC CTGGGCCCAA TGACTCTGCT TGTGTTTCCT GGATCTATTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA TAGTGGCCTG GTGGGGCCCT TGGCTATCTG CCAAAAGGGC 
ATCCTGGAGC CCCATGGAGG ACGGAGTGAC ATGGATCGGG AATTTGCATT GTTGTTCTTG 
ATTTTTGATG AAAATAAGTC TTGGTATTTG GAGGAAAATG TGGCAACCCA TGGGTCCCAG 
GATCCAGGCA GTATTAACCT ACAGGATGAA ACTTTCTTGG AGAGCAATAA AATGCATGCA 
ATCAATGGGA AACTCTATGC CAACCTTAGG GGTCTTACCA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGCTGGCCAT GGGCCAAGAT GTGGATCTAC ACACCATCCA CTTTCATGCA 
GAGAGCTTCC TCTATCGGAA TGGCGAGAAC TACCGGGCAG ATGTGGTGGA TCTGTTCCCA 
GGGACTTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GATGCACTGC 
CATGTGACTG ACCATGTCCA TGCTGGCATG GAGACCCTCT TCACTGTTTT TTCTCGAACA 
GAACACTTAA GCCCTCTCAC CGTCATCACC AAAGAGACTG AAAAAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529375.1
CDS	202..3837
Translation	MTQTLPYHLSLLNVLFPGPCSRHFKFRRGKCAQPAWRKVSAPSQDLLITKVMWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_011531073.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531073.2

ORF Insert Sequence:

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGGTGAG GTGGTCACTT ATCAGTGGAA CATCCCAGAG 
AGGTCTGGCC CTGGGCCCAA TGACTCTGCT TGTGTTTCCT GGATCTATTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA TAGTGGCCTG GTGGGGCCCT TGGCTATCTG CCAAAAGGGC 
ATCCTGGAGC CCCATGGAGG ACGGAGTGAC ATGGATCGGG AATTTGCATT GTTGTTCTTG 
ATTTTTGATG AAAATAAGTC TTGGTATTTG GAGGAAAATG TGGCAACCCA TGGGTCCCAG 
GATCCAGGCA GTATTAACCT ACAGGATGAA ACTTTCTTGG AGAGCAATAA AATGCATGCA 
ATCAATGGGA AACTCTATGC CAACCTTAGG GGTCTTACCA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGCTGGCCAT GGGCCAAGAT GTGGATCTAC ACACCATCCA CTTTCATGCA 
GAGAGCTTCC TCTATCGGAA TGGCGAGAAC TACCGGGCAG ATGTGGTGGA TCTGTTCCCA 
GGGACTTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GATGCACTGC 
CATGTGACTG ACCATGTCCA TGCTGGCATG GAGACCCTCT TCACTGTTTT TTCTCGAACA 
GAACACTTAA GCCCTCTCAC CGTCATCACC AAAGAGACTG AAAAAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_011531073.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529375.1 Homo sapiens hephaestin (HEPH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu63368
Clone ID Related Accession (Same CDS sequence)	XM_011531073.2 , XM_011531073.3
Accession Version	XM_011531073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011531073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGGTGAG GTGGTCACTT ATCAGTGGAA CATCCCAGAG 
AGGTCTGGCC CTGGGCCCAA TGACTCTGCT TGTGTTTCCT GGATCTATTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA TAGTGGCCTG GTGGGGCCCT TGGCTATCTG CCAAAAGGGC 
ATCCTGGAGC CCCATGGAGG ACGGAGTGAC ATGGATCGGG AATTTGCATT GTTGTTCTTG 
ATTTTTGATG AAAATAAGTC TTGGTATTTG GAGGAAAATG TGGCAACCCA TGGGTCCCAG 
GATCCAGGCA GTATTAACCT ACAGGATGAA ACTTTCTTGG AGAGCAATAA AATGCATGCA 
ATCAATGGGA AACTCTATGC CAACCTTAGG GGTCTTACCA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGCTGGCCAT GGGCCAAGAT GTGGATCTAC ACACCATCCA CTTTCATGCA 
GAGAGCTTCC TCTATCGGAA TGGCGAGAAC TACCGGGCAG ATGTGGTGGA TCTGTTCCCA 
GGGACTTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GATGCACTGC 
CATGTGACTG ACCATGTCCA TGCTGGCATG GAGACCCTCT TCACTGTTTT TTCTCGAACA 
GAACACTTAA GCCCTCTCAC CGTCATCACC AAAGAGACTG AAAAAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529375.1
CDS	203..3838
Translation	MTQTLPYHLSLLNVLFPGPCSRHFKFRRGKCAQPAWRKVSAPSQDLLITKVMWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_011531073.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531073.3

ORF Insert Sequence:

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGGTGAG GTGGTCACTT ATCAGTGGAA CATCCCAGAG 
AGGTCTGGCC CTGGGCCCAA TGACTCTGCT TGTGTTTCCT GGATCTATTA TTCTGCAGTG 
GATCCCATCA AGGACATGTA TAGTGGCCTG GTGGGGCCCT TGGCTATCTG CCAAAAGGGC 
ATCCTGGAGC CCCATGGAGG ACGGAGTGAC ATGGATCGGG AATTTGCATT GTTGTTCTTG 
ATTTTTGATG AAAATAAGTC TTGGTATTTG GAGGAAAATG TGGCAACCCA TGGGTCCCAG 
GATCCAGGCA GTATTAACCT ACAGGATGAA ACTTTCTTGG AGAGCAATAA AATGCATGCA 
ATCAATGGGA AACTCTATGC CAACCTTAGG GGTCTTACCA TGTACCAAGG AGAACGAGTG 
GCCTGGTACA TGCTGGCCAT GGGCCAAGAT GTGGATCTAC ACACCATCCA CTTTCATGCA 
GAGAGCTTCC TCTATCGGAA TGGCGAGAAC TACCGGGCAG ATGTGGTGGA TCTGTTCCCA 
GGGACTTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GATGCACTGC 
CATGTGACTG ACCATGTCCA TGCTGGCATG GAGACCCTCT TCACTGTTTT TTCTCGAACA 
GAACACTTAA GCCCTCTCAC CGTCATCACC AAAGAGACTG AAAAAGTGCC CCCCAGAGAC 
ATTGAAGAAG GCAATGTGAA GATGCTGGGC ATGCAGATCC CCATAAAGAA TGTTGAGATG 
CTGGCCTCTG TTTTGGTTGC CATTAGTGTC ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT 
GGAGTGGTTT GGTACCAACA TCGACAGAGA AAGCTACGAC GCAATAGGAG GTCCATCCTG 
GATGACAGCT TCAAGCTTCT GTCTTTCAAA CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_011531074.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529376.1 Homo sapiens hephaestin (HEPH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu63369
Clone ID Related Accession (Same CDS sequence)	XM_011531074.2
Accession Version	XM_011531074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529376.1
CDS	202..3627
Translation	MTQTLPYHLSLLNVLFPGPCSRHFKFRRGKCAQPAWRKVSAPSQDLLITKVMWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_011531074.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531074.2

ORF Insert Sequence:

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGGGAC ATTTGAGATT 
TATTGCCAGG CAGGCAGCCA TCGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCAG 
TGTCCTGGCC ACCAAGCCAC CCCTCGCCAA CGCTACCAAG CTGCAAGAAT CTACTATATC 
ATGGCAGAAG AAGTAGAGTG GGACTATTGC CCTGACCGGA GCTGGGAACG GGAATGGCAC 
AACCAGTCTG AGAAGGACAG TTATGGTTAC ATTTTCCTGA GCAACAAGGA TGGGCTCCTG 
GGTTCCAGAT ACAAGAAAGC TGTATTCAGG GAATACACTG ATGGTACATT CAGGATCCCT 
CGGCCAAGGA CTGGACCAGA AGAACACTTG GGAATCTTGG GTCCACTTAT CAAAGGTGAA 
GTTGGTGATA TCCTGACTGT GGTATTCAAG AATAATGCCA GCCGCCCCTA CTCTGTGCAT 
GCTCATGGAG TGCTAGAATC TACTACTGTC TGGCCACTGG CTGCTGAGCC TGGTGAGGTG 
GTCACTTATC AGTGGAACAT CCCAGAGAGG TCTGGCCCTG GGCCCAATGA CTCTGCTTGT 
GTTTCCTGGA TCTATTATTC TGCAGTGGAT CCCATCAAGG ACATGTATAG TGGCCTGGTG 
GGGCCCTTGG CTATCTGCCA AAAGGGCATC CTGGAGCCCC ATGGAGGACG GAGTGACATG 
GATCGGGAAT TTGCATTGTT GTTCTTGATT TTTGATGAAA ATAAGTCTTG GTATTTGGAG 
GAAAATGTGG CAACCCATGG GTCCCAGGAT CCAGGCAGTA TTAACCTACA GGATGAAACT 
TTCTTGGAGA GCAATAAAAT GCATGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPH ( XM_011531075.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529377.1 Homo sapiens hephaestin (HEPH), transcript variant X5, mRNA.

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CloneID	OHu63370
Clone ID Related Accession (Same CDS sequence)	XM_011531075.2
Accession Version	XM_011531075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hephaestin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529377.1
CDS	202..3507
Translation	MTQTLPYHLSLLNVLFPGPCSRHFKFRRGKCAQPAWRKVSAPSQDLLITKVMWAMESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ

Target ORF information:

RefSeq Version	XM_011531075.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hephaestin (HEPH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531075.2

ORF Insert Sequence:

ATGACCCAGA CTTTGCCCTA CCACCTCAGT CTTCTGAATG TTCTCTTCCC TGGACCCTGC 
TCCAGACACT TTAAATTCAG AAGAGGAAAA TGTGCCCAGC CTGCCTGGAG AAAAGTGTCT 
GCTCCTAGCC AAGATCTCCT CATCACAAAA GTAATGTGGG CCATGGAGTC AGGCCACCTC 
CTCTGGGCTC TGCTGTTCAT GCAGTCCTTG TGGCCTCAAC TGACTGATGG AGCCACTCGA 
GTCTACTACC TGGGCATCCG GGATGTGCAG TGGAACTATG CTCCCAAGGG AAGAAATGTC 
ATCACGAACC AGCCTCTGGA CAGTGACATA GTGGCTTCCA GCTTCTTAAA GTCTGACAAG 
AACCGGATAG GGGGAACCTA CAAGAAGACC ATCTATAAAG AATACAAGGA TGACTCATAC 
ACAGATGAAG TGGCCCAGCC TGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GCAGGCTGAA 
GTGGGGGATG TCATTCTTAT TCACCTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAC 
CCTCATGGTG TCTTCTACGA GAAGGACTCT GAAGGTTCCC TATACCCAGA TGGCTCCTCT 
GGGCCACTGA AAGCTGATGA CTCTGTTCCC CCGGGGGGCA GCCATATCTA CAACTGGACC 
ATTCCAGAAG GCCATGCACC CACCGATGCT GACCCAGCGT GCCTCACCTG GATCTACCAT 
TCTCATGTAG ATGCTCCACG AGACATTGCA ACTGGCCTAA TTGGGCCTCT CATCACCTGT 
AAAAGAGGAG CCCTGGATGG GAACTCCCCT CCTCAACGCC AGGATGTAGA CCATGATTTC 
TTCCTCCTCT TCAGTGTGGT AGATGAGAAC CTCAGCTGGC ATCTCAATGA GAACATTGCC 
ACTTACTGCT CAGATCCTGC TTCAGTGGAC AAAGAAGATG AGACATTTCA GGAGAGCAAT 
AGGATGCATG CAATCAATGG CTTTGTTTTT GGGAATTTAC CTGAGCTGAA CATGTGTGCA 
CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AAATTGATGT CCACACAGCA 
TTTTTCCATG GACAGATGCT GACTACCCGT GGACACCACA CTGATGTGGC TAACATCTTT 
CCAGCCACCT TTGTGACTGC TGAGATGGTG CCCTGGGAAC CTGGTACCTG GTTAATTAGC 
TGCCAAGTGA ACAGTCACTT TCGAGATGGC ATGCAGGCAC TCTACAAGGT CAAGTCTTGC 
TCCATGGCCC CTCCTGTGGA CCTGCTCACA GGCAAAGTTC GACAGTACTT CATTGAGGCC 
CATGAGATTC AATGGGACTA TGGCCCGATG GGGCATGATG GGAGTACTGG GAAGAATTTG 
AGAGAGCCAG GCAGTATCTC AGATAAGTTT TTCCAGAAGA GCTCCAGCCG AATTGGGGGC 
ACTTACTGGA AAGTGCGATA TGAAGCCTTT CAAGATGAGA CATTCCAAGA GAAGATGCAT 
TTGGAGGAAG ATAGGCATCT TGGAATCCTG GGGCCAGTGA TCCGGGCTGA GGTGGGTGAC 
ACCATTCAGG TGGTCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATGCA GCCCCATGGG 
GTCTTTTATG AGAAAGACTA TGAAGGCACT GTGTACAATG ATGGCTCATC TTACCCTGGC 
TTGGTTGCCA AGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCCCC TCATGCCGGT 
CCCACTGCTC AGGATCCTGC TTGTCTCACT TGGATGTACT TCTCTGCTGC AGATCCCATA 
AGAGACACAA ATTCTGGCCT GGTGGGCCCG CTGCTGGTGT GCAGGGCTGG TGCCTTGGGT 
GCAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TTCTCTTCAC TGTGTTGGAT 
GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTTCCGACTG 
CTTTCAGAGG ATATTGAGGG CTTCCAAGAC TCCAATCGGA TGCATGCCAT TAATGGGTTT 
CTGTTCTCTA ACCTGCCCAG GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG 
CTCGGCCTGG GCACAGAGAC TGATGTGCAT GGAGTCATGT TCCAGGGCAA CACTGTGCAG 
CTTCAGGGCA TGAGGAAGGG TGCAGCTATG CTCTTTCCTC ATACCTTTGT CATGGCCATC 
ATGCAGCCTG ACAACCTTGG GACATTTGAG ATTTATTGCC AGGCAGGCAG CCATCGAGAA 
GCAGGGATGA GGGCAATCTA TAATGTCTCC CAGTGTCCTG GCCACCAAGC CACCCCTCGC 
CAACGCTACC AAGCTGCAAG AATCTACTAT ATCATGGCAG AAGAAGTAGA GTGGGACTAT 
TGCCCTGACC GGAGCTGGGA ACGGGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT 
TACATTTTCC TGAGCAACAA GGATGGGCTC CTGGGTTCCA GATACAAGAA AGCTGTATTC 
AGGGAATACA CTGATGGTAC ATTCAGGATC CCTCGGCCAA GGACTGGACC AGAAGAACAC 
TTGGGAATCT TGGGTCCACT TATCAAAGGT GAAGTTGGTG ATATCCTGAC TGTGGTATTC 
AAGAATAATG CCAGCCGCCC CTACTCTGTG CATGCTCATG GAGTGCTAGA ATCTACTACT 
GTCTGGCCAC TGGCTGCTGA GCCTGCAATC AATGGGAAAC TCTATGCCAA CCTTAGGGGT 
CTTACCATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGC TGGCCATGGG CCAAGATGTG 
GATCTACACA CCATCCACTT TCATGCAGAG AGCTTCCTCT ATCGGAATGG CGAGAACTAC 
CGGGCAGATG TGGTGGATCT GTTCCCAGGG ACTTTTGAGG TTGTGGAGAT GGTGGCCAGC 
AACCCTGGGA CATGGCTGAT GCACTGCCAT GTGACTGACC ATGTCCATGC TGGCATGGAG 
ACCCTCTTCA CTGTTTTTTC TCGAACAGAA CACTTAAGCC CTCTCACCGT CATCACCAAA 
GAGACTGAAA AAGCAGTGCC CCCCAGAGAC ATTGAAGAAG GCAATGTGAA GATGCTGGGC 
ATGCAGATCC CCATAAAGAA TGTTGAGATG CTGGCCTCTG TTTTGGTTGC CATTAGTGTC 
ACCCTTCTGC TCGTTGTTCT GGCTCTTGGT GGAGTGGTTT GGTACCAACA TCGACAGAGA 
AAGCTACGAC GCAATAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTTTCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Ceruloplasmin, hephaestin and zyklopen: the three multicopper oxidases important for human iron metabolism].
Postepy higieny i medycyny doswiadczalnej (Online)68912-24(2014)
Wierzbicka D,Gromadzka G

Ferroportin and exocytoplasmic ferroxidase activity are required for brain microvascular endothelial cell iron efflux.
The Journal of biological chemistry288(24)17932-40(2013 Jun)
McCarthy RC,Kosman DJ

Functional role of the putative iron ligands in the ferroxidase activity of recombinant human hephaestin.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry17(8)1187-95(2012 Dec)
Vashchenko G,Macgillivray RT

An X chromosome association scan of the Norfolk Island genetic isolate provides evidence for a novel migraine susceptibility locus at Xq12.
PloS one7(5)e37903(2012)
Maher BH,Lea RA,Benton M,Cox HC,Bellis C,Carless M,Dyer TD,Curran J,Charlesworth JC,Buring JE,Kurth T,Chasman DI,Ridker PM,Sch?rks M,Blangero J,Griffiths LR

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HEPH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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