HEPH cDNA ORF clone, Bos taurus(cattle)

The following HEPH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEPH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa35673 XM_005228270.4
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa35673 XM_015470504.1
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47792 XM_015470505.1
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47792 XM_010822045.3
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa35668 XM_005228266.4
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35668 XM_015470498.1
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35669 XM_015470499.1
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35669 XM_002700167.6
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35671 XM_015470500.1
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa35671 XM_015470501.1
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa35671 XM_015461727.2
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa35671 XM_010822042.3
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa35672 XM_015470503.1
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa35672 XM_005228269.4
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47790 XM_010822043.3
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47790 XM_015470502.1
Latest version!
Bos taurus hephaestin (HEPH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa35668 XM_005228266.3 Bos taurus hephaestin (HEPH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35669 XM_002700167.5 Bos taurus hephaestin (HEPH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa35672 XM_005228269.3 Bos taurus hephaestin (HEPH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa35671 XM_015461727.1 Bos taurus hephaestin (HEPH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa47790 XM_010822043.2 Bos taurus hephaestin (HEPH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa35673 XM_005228270.3 Bos taurus hephaestin (HEPH), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47792 XM_010822045.2 Bos taurus hephaestin (HEPH), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa35671 XM_010822042.2 Bos taurus hephaestin (HEPH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa35673
    Clone ID Related Accession (Same CDS sequence) XM_015470504.1 , XM_005228270.3 , XM_005228270.4
    Accession Version XM_015470504.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA
    GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT
    TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG
    GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC
    AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325990.1
    CDS268..3177
    Translation

    Target ORF information:

    RefSeq Version XM_015470504.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA
    GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT
    TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG
    GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC
    AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35673
    Clone ID Related Accession (Same CDS sequence) XM_015470504.1 , XM_005228270.3 , XM_005228270.4
    Accession Version XM_005228270.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228270.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA
    GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT
    TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG
    GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC
    AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228327.1
    CDS268..3177
    Translation

    Target ORF information:

    RefSeq Version XM_005228270.3
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228270.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA
    GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT
    TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG
    GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC
    AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35673
    Clone ID Related Accession (Same CDS sequence) XM_015470504.1 , XM_005228270.3 , XM_005228270.4
    Accession Version XM_005228270.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hephaestin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228270.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA
    GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT
    TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG
    GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC
    AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228327.1
    CDS271..3180
    Translation

    Target ORF information:

    RefSeq Version XM_005228270.4
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228270.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGTAG CAATTCCCAG AAACATTGGA
    GAAGGCAGTG TGAAGATGCT GGGCATGCAA ATCCCTATAA AGGATATTGA GATGCTGACT
    TCTGTCTTGA TTGCCATGGG TGTCATTCTC CTGCTCATTG CTCTGGTTCT TGGAGGTGTG
    GTGTGGTACC AGCATCGTCA GAGGAAGTTA CGGCGCAACA GGAGGTCCAT CCTGGATGAC
    AGCTTCAAGC TTCTGTCTCT CAAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47792
    Clone ID Related Accession (Same CDS sequence) XM_010822045.3 , XM_015470505.1 , XM_010822045.2
    Accession Version XM_010822045.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hephaestin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010822045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA
    GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT
    GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG
    TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC
    TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820347.1
    CDS271..3177
    Translation

    Target ORF information:

    RefSeq Version XM_010822045.3
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822045.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA
    GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT
    GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG
    TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC
    TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47792
    Clone ID Related Accession (Same CDS sequence) XM_010822045.3 , XM_015470505.1 , XM_010822045.2
    Accession Version XM_015470505.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA
    GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT
    GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG
    TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC
    TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325991.1
    CDS269..3175
    Translation

    Target ORF information:

    RefSeq Version XM_015470505.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470505.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA
    GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT
    GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG
    TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC
    TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47792
    Clone ID Related Accession (Same CDS sequence) XM_010822045.3 , XM_015470505.1 , XM_010822045.2
    Accession Version XM_010822045.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA
    GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT
    GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG
    TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC
    TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820347.1
    CDS269..3175
    Translation

    Target ORF information:

    RefSeq Version XM_010822045.2
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822045.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGAACACTTA
    AGCACTACCA CCACCATCCC TGAAGAGATT GGAAAAGCAA TTCCCAGAAA CATTGGAGAA
    GGCAGTGTGA AGATGCTGGG CATGCAAATC CCTATAAAGG ATATTGAGAT GCTGACTTCT
    GTCTTGATTG CCATGGGTGT CATTCTCCTG CTCATTGCTC TGGTTCTTGG AGGTGTGGTG
    TGGTACCAGC ATCGTCAGAG GAAGTTACGG CGCAACAGGA GGTCCATCCT GGATGACAGC
    TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35668
    Clone ID Related Accession (Same CDS sequence) XM_005228266.3 , XM_005228266.4 , XM_015470498.1
    Accession Version XM_005228266.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228266.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC
    ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC
    ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG
    AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228323.1
    CDS265..3810
    Translation

    Target ORF information:

    RefSeq Version XM_005228266.3
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228266.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC
    ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC
    ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG
    AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35668
    Clone ID Related Accession (Same CDS sequence) XM_005228266.3 , XM_005228266.4 , XM_015470498.1
    Accession Version XM_005228266.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hephaestin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228266.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC
    ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC
    ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG
    AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228323.1
    CDS263..3808
    Translation

    Target ORF information:

    RefSeq Version XM_005228266.4
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228266.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC
    ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC
    ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG
    AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35668
    Clone ID Related Accession (Same CDS sequence) XM_005228266.3 , XM_005228266.4 , XM_015470498.1
    Accession Version XM_015470498.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC
    ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC
    ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG
    AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325984.1
    CDS265..3810
    Translation

    Target ORF information:

    RefSeq Version XM_015470498.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470498.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGTAGCAAT TCCCAGAAAC ATTGGAGAAG GCAGTGTGAA GATGCTGGGC
    ATGCAAATCC CTATAAAGGA TATTGAGATG CTGACTTCTG TCTTGATTGC CATGGGTGTC
    ATTCTCCTGC TCATTGCTCT GGTTCTTGGA GGTGTGGTGT GGTACCAGCA TCGTCAGAGG
    AAGTTACGGC GCAACAGGAG GTCCATCCTG GATGACAGCT TCAAGCTTCT GTCTCTCAAG
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35669
    Clone ID Related Accession (Same CDS sequence) XM_002700167.5 , XM_015470499.1 , XM_002700167.6
    Accession Version XM_002700167.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700167.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG
    CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT
    CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG
    TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002700213.3
    CDS265..3807
    Translation

    Target ORF information:

    RefSeq Version XM_002700167.5
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002700167.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG
    CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT
    CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG
    TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35669
    Clone ID Related Accession (Same CDS sequence) XM_002700167.5 , XM_015470499.1 , XM_002700167.6
    Accession Version XM_015470499.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG
    CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT
    CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG
    TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325985.1
    CDS265..3807
    Translation

    Target ORF information:

    RefSeq Version XM_015470499.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470499.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG
    CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT
    CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG
    TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35669
    Clone ID Related Accession (Same CDS sequence) XM_002700167.5 , XM_015470499.1 , XM_002700167.6
    Accession Version XM_002700167.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hephaestin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002700167.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG
    CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT
    CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG
    TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002700213.3
    CDS268..3810
    Translation

    Target ORF information:

    RefSeq Version XM_002700167.6
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002700167.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGCCATTAA TGGGTTTCTG TTCTCTAATC TGCCCAAGCT GGACATGTGC
    AAGGGTGACA CAGTGGCCTG GCACCTGCTT GGCCTGGGCA CAGAGACTGA TGTGCACGGG
    GTCACATTCC AGGGCAACAC TGTGCAGCTT CAGGGCATGA GGAAGAGTGC AGCCACGCTC
    TTTCCTCACA CCTTTGTTAT GGCCATCATG CAGCCTGACA ACCCTGGGAC ATTTGAAATT
    TACTGCCAAG CAGGCAGCCA TAGAGAAGCA GGGATGAGGG CAATCTATAA TGTCTCCCTT
    TGTCCTGGAC ACCAAGCCAA TCCTCATCAA CGTTACCAAG CTGCAAGGAT CTACTACATC
    ATGGCAGAAG AGATGGAGTG GGACTATTGC CCAGACAGGA GCTGGGAACT AGAATGGCAC
    AACCAGTCTG AGAAGGACAG TTATGGTCAC ATCTTTCTGA GCAACAAAGA TGGACTCCTG
    GGTTCAAGAT ACAAGAAAGC TGTATTCAGA GAGTATACTG ATGGCACATT CAGGGTCCAA
    CGGCCAAGGA CTGGATCAGA GGAACACTTG GGAATCTTAG GTCCACTTAT CAGAGGAGAG
    GTTGGTGATA TTCTAACTGT GGTGTTCAAG AACAATGCCA GCCGCCCCTA CTCTGTGCAT
    GCCCATGGAG TGCTAGAATC CAGCACTGGT TGGCCACTGG CTGCTGAGCC TGGTGAGGTT
    CTCACTTACC AATGGAACAT TCCAGAGAGA TCTGGCCCTG GTCTCAATGA TTCTGCTTGT
    GTTTCTTGGA TCTACTATTC TGCAGTGGAT CCCATCAAGG ACATGTACAG TGGCCTGGTG
    GGACCCTTGG CCATCTGCCG AAAAGGCATC CTGGAGCCCT ACGGAGGACG ACGTGACATG
    AACCGAGAAT TTGCCTTGTT ATTCTTGATT TTTGATGAGA ACAAGTCTTG GTATCTGGAG
    GAGAATGTGG AAACTTATGG GTCCCAGGAT CCAGGCCGTG TTAACCTACA GGATGAGACT
    TTCTTGGAGA GCAATAAAAT GCATGCCATT AATGGAAAGC TCTATGCCAA CCTCATGGGC
    CTTACAATGT ACCAAGGAGA ACGAGTGGCC TGGTACATGA TGGCAATGGG ACAAGATATT
    GACCTACACA CTGTCCATTT CCATGCAGAA AGCTTTCTCT ACCGGAGTGG AGAGAGCTAC
    CGGGCAGATG TAGTGGATTT GTTTCCAGGG ACCTTTGAGG TTGTGGAGAT GGTGGCCAGC
    AACCCTGGGA CATGGCTGCT TCACTGCCAT GTTACTGACC ATGTCCATGC TGGCATGGAG
    ACAGTCTTTA CGGTCTTGTC CCAAAGAGAA CACTTAAGCA CTACCACCAC CATCCCTGAA
    GAGATTGGAA AAGCAATTCC CAGAAACATT GGAGAAGGCA GTGTGAAGAT GCTGGGCATG
    CAAATCCCTA TAAAGGATAT TGAGATGCTG ACTTCTGTCT TGATTGCCAT GGGTGTCATT
    CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT GTGGTGTGGT ACCAGCATCG TCAGAGGAAG
    TTACGGCGCA ACAGGAGGTC CATCCTGGAT GACAGCTTCA AGCTTCTGTC TCTCAAGCAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35671
    Clone ID Related Accession (Same CDS sequence) XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
    Accession Version XM_015461727.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hephaestin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317213.1
    CDS89..3565
    Translation

    Target ORF information:

    RefSeq Version XM_015461727.2
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461727.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35671
    Clone ID Related Accession (Same CDS sequence) XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
    Accession Version XM_015461727.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317213.1
    CDS101..3577
    Translation

    Target ORF information:

    RefSeq Version XM_015461727.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35671
    Clone ID Related Accession (Same CDS sequence) XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
    Accession Version XM_010822042.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820344.1
    CDS308..3784
    Translation

    Target ORF information:

    RefSeq Version XM_010822042.2
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822042.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35671
    Clone ID Related Accession (Same CDS sequence) XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
    Accession Version XM_015470500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325986.1
    CDS101..3577
    Translation

    Target ORF information:

    RefSeq Version XM_015470500.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35671
    Clone ID Related Accession (Same CDS sequence) XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
    Accession Version XM_015470501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325987.1
    CDS308..3784
    Translation

    Target ORF information:

    RefSeq Version XM_015470501.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35671
    Clone ID Related Accession (Same CDS sequence) XM_015461727.2 , XM_015461727.1 , XM_010822042.2 , XM_015470500.1 , XM_015470501.1 , XM_010822042.3
    Accession Version XM_010822042.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hephaestin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010822042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820344.1
    CDS310..3786
    Translation

    Target ORF information:

    RefSeq Version XM_010822042.3
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822042.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGTCAG GCCATCTCCT CTGGGCTCTG CTGTTCATGC AACCTCTGTG GCCCCAACTG 
    ACTGATGGGA CTACTAGAGT CTACTACCTG GGGATCCAGG ATGTGCGGTG GAACTATGCT
    CCTAAGGGAA GGAATATCAT CACGAACCAG CCTCTGGAAA GTGACATAGT AGCTGCTAGC
    TTCTTGAAGT CTGACAAGAA CCGAATAGGG AACAGCTACA AGAAGACCAT CTACAAGGAA
    TACAAGGATG GCTCATACAT GGATGAAATG GTCCAGCCAG CCTGGTTGGG CTTCCTGGGG
    CCAGTGTTGA GGGCTGAGGT GGGAGATGTC ATCCTTATCC ACTTGAAGAA TTTTGCCACT
    CGTCCCTATA CCATCCATCC CCATGGTGTA TTCTATGAAA AGGACTCAGA AGGTTCCTTG
    TATCCAGATG GTTCCTCTGG TTGGTTGAAA GCTGATGATT CTGTACCCCC AGGAGGCAGC
    CACATCTACA ACTGGACCAT TCCAGAAGGC CATGCACCCA CTGATGCTGA CCCAGCATGC
    CTCACCTGGG TCTACCATTC TCATGTAGAT GCACCACGAG ATATTGCTAC TGGTCTCATT
    GGACCCCTTA TAACCTGTAA AAGAGGAACC CTGGATGGGA GCTTCCCTCC TCGGCGGCAG
    GATGTGGACA ATGATTTCTT CCTCCTTTTT AGTGTGATAG ATGAGAACCT TAGCTGGCAT
    ATCAATGAGA ACATTGCCAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGAG
    GACTTTCAGA AGAGCAATAG GATGCACGCA ATAAATGGCT TTGTATTTGG AAACTTGCCA
    GATCTAAGTA TGTGTGCACA GAAACGTGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG
    GTAGATGTCC ACACAGTTTT CTTCCATGGA CAGATGCTGA CCATCCGAGG TCATAGAACT
    GATGTGGCTC ATATCTTTCC AGCCACTTTT GTGAGTGCTG AGATGGTGCC CCAGGAACCT
    GGCACCTGGT TAATCAGCTG CCAAGTGAAT AGTCATTTGC AAGATGGCAT GCAAGCACTC
    TACAAGGTCA AGTCTTGCTC CATGGCACCT CCCATGAACT TACTCACAGG CAAAGTCCGG
    CAGTACTTCA TTGAGGCCCA TGAGATTCAA TGGGACTATG GCCCTATGGG GCATGATGGG
    AGTACGGGAA AGAACTTGAG GGAGCCAGGC AGTGGCTCAG ATAAATTCTT CCAGAAGAGC
    TCCATCCGAA TTGGGGGCAC TTACTGGAAA GTTCGATATG AAGCCTTCCA AGATGAGACA
    TTCCAGGAAA AGGTGCAGCT GGAGGAGGAT AAGCATCTTG GAATTCTGGG ACCAGTGATC
    CGGGCTGAGG TGGGTGACAC CATCCTGGTG ATCTTCTACA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CTCATGGAGT CTTTTATGAG AAGGACTCTG AGGGAACTGT GTACAATGAT
    GACTCTTCCC ACTCTGGCTT AGTGGCTAGG CCCTTTGAGA AAGTAACATA CCGCTGGACA
    GTCCCTCCCC ATGCTGGTCC CACTGTTCAG GATCCTGCCT GTCTCACCTG GACATACTTC
    TCTGCTGCAG ATCCCATAAG GGATACCAAT TCTGGTCTGG TGGGCCCCCT GCTGGTGTGC
    AAGGCTGGCA CCTTGGGTAC AGATGGCAAG CAGAAAGGGG TGGATAAAGA ATTCTTTCTC
    CTTTTCACTG TGTTGGATGA GAACAAGAGC TGGTACAGCA ATGCCAATCA AGCAGCTGCT
    ATGTTGGATT CCCGACTGCT CTCAGAGGAT GCCAAGGGTT TTCAAGACTC TAATCGGATG
    CATGCCATTA ATGGGTTTCT GTTCTCTAAT CTGCCCAAGC TGGACATGTG CAAGGGTGAC
    ACAGTGGCCT GGCACCTGCT TGGCCTGGGC ACAGAGACTG ATGTGCACGG GGTCACATTC
    CAGGGCAACA CTGTGCAGCT TCAGGGCATG AGGAAGAGTG CAGCCACGCT CTTTCCTCAC
    ACCTTTGTTA TGGCCATCAT GCAGCCTGAC AACCCTGGGA CATTTGAAAT TTACTGCCAA
    GCAGGCAGCC ATAGAGAAGC AGGGATGAGG GCAATCTATA ATGTCTCCCT TTGTCCTGGA
    CACCAAGCCA ATCCTCATCA ACGTTACCAA GCTGCAAGGA TCTACTACAT CATGGCAGAA
    GAGATGGAGT GGGACTATTG CCCAGACAGG AGCTGGGAAC TAGAATGGCA CAACCAGTCT
    GAGAAGGACA GTTATGGTCA CATCTTTCTG AGCAACAAAG ATGGACTCCT GGGTTCAAGA
    TACAAGAAAG CTGTATTCAG AGAGTATACT GATGGCACAT TCAGGGTCCA ACGGCCAAGG
    ACTGGATCAG AGGAACACTT GGGAATCTTA GGTCCACTTA TCAGAGGAGA GGTTGGTGAT
    ATTCTAACTG TGGTGTTCAA GAACAATGCC AGCCGCCCCT ACTCTGTGCA TGCCCATGGA
    GTGCTAGAAT CCAGCACTGG TTGGCCACTG GCTGCTGAGC CTGGTGAGGT TCTCACTTAC
    CAATGGAACA TTCCAGAGAG ATCTGGCCCT GGTCTCAATG ATTCTGCTTG TGTTTCTTGG
    ATCTACTATT CTGCAGTGGA TCCCATCAAG GACATGTACA GTGGCCTGGT GGGACCCTTG
    GCCATCTGCC GAAAAGGCAT CCTGGAGCCC TACGGAGGAC GACGTGACAT GAACCGAGAA
    TTTGCCTTGT TATTCTTGAT TTTTGATGAG AACAAGTCTT GGTATCTGGA GGAGAATGTG
    GAAACTTATG GGTCCCAGGA TCCAGGCCGT GTTAACCTAC AGGATGAGAC TTTCTTGGAG
    AGCAATAAAA TGCATGCCAT TAATGGAAAG CTCTATGCCA ACCTCATGGG CCTTACAATG
    TACCAAGGAG AACGAGTGGC CTGGTACATG ATGGCAATGG GACAAGATAT TGACCTACAC
    ACTGTCCATT TCCATGCAGA AAGCTTTCTC TACCGGAGTG GAGAGAGCTA CCGGGCAGAT
    GTAGTGGATT TGTTTCCAGG GACCTTTGAG GTTGTGGAGA TGGTGGCCAG CAACCCTGGG
    ACATGGCTGC TTCACTGCCA TGTTACTGAC CATGTCCATG CTGGCATGGA GACAGTCTTT
    ACGGTCTTGT CCCAAAGAGA ACACTTAAGC ACTACCACCA CCATCCCTGA AGAGATTGGA
    AAAGTAGCAA TTCCCAGAAA CATTGGAGAA GGCAGTGTGA AGATGCTGGG CATGCAAATC
    CCTATAAAGG ATATTGAGAT GCTGACTTCT GTCTTGATTG CCATGGGTGT CATTCTCCTG
    CTCATTGCTC TGGTTCTTGG AGGTGTGGTG TGGTACCAGC ATCGTCAGAG GAAGTTACGG
    CGCAACAGGA GGTCCATCCT GGATGACAGC TTCAAGCTTC TGTCTCTCAA GCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35672
    Clone ID Related Accession (Same CDS sequence) XM_005228269.4 , XM_005228269.3 , XM_015470503.1
    Accession Version XM_005228269.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hephaestin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228269.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
    GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT
    CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT
    GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC
    ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT
    TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT
    AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC
    TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC
    ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT
    AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA
    CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT
    TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT
    CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC
    TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC
    TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC
    CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG
    AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC
    ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG
    CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC
    ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA
    GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC
    TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT
    CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA
    AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT
    ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT
    GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG
    CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT
    CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG
    CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG
    CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC
    ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA
    GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT
    CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT
    TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT
    CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC
    AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC
    TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC
    AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT
    GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG
    AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG
    GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC
    ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG
    ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG
    GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC
    ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG
    GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA
    GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA
    GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC
    CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA
    GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA
    AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG
    ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT
    GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC
    CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228326.1
    CDS387..3605
    Translation

    Target ORF information:

    RefSeq Version XM_005228269.4
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228269.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
    GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT
    CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT
    GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC
    ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT
    TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT
    AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC
    TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC
    ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT
    AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA
    CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT
    TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT
    CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC
    TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC
    TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC
    CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG
    AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC
    ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG
    CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC
    ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA
    GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC
    TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT
    CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA
    AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT
    ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT
    GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG
    CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT
    CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG
    CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG
    CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC
    ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA
    GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT
    CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT
    TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT
    CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC
    AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC
    TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC
    AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT
    GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG
    AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG
    GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC
    ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG
    ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG
    GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC
    ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG
    GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA
    GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA
    GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC
    CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA
    GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA
    AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG
    ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT
    GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC
    CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35672
    Clone ID Related Accession (Same CDS sequence) XM_005228269.4 , XM_005228269.3 , XM_015470503.1
    Accession Version XM_005228269.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228269.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
    GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT
    CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT
    GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC
    ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT
    TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT
    AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC
    TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC
    ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT
    AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA
    CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT
    TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT
    CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC
    TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC
    TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC
    CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG
    AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC
    ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG
    CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC
    ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA
    GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC
    TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT
    CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA
    AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT
    ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT
    GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG
    CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT
    CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG
    CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG
    CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC
    ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA
    GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT
    CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT
    TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT
    CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC
    AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC
    TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC
    AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT
    GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG
    AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG
    GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC
    ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG
    ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG
    GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC
    ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG
    GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA
    GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA
    GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC
    CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA
    GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA
    AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG
    ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT
    GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC
    CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228326.1
    CDS385..3603
    Translation

    Target ORF information:

    RefSeq Version XM_005228269.3
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228269.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
    GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT
    CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT
    GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC
    ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT
    TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT
    AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC
    TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC
    ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT
    AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA
    CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT
    TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT
    CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC
    TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC
    TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC
    CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG
    AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC
    ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG
    CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC
    ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA
    GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC
    TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT
    CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA
    AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT
    ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT
    GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG
    CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT
    CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG
    CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG
    CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC
    ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA
    GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT
    CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT
    TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT
    CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC
    AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC
    TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC
    AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT
    GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG
    AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG
    GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC
    ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG
    ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG
    GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC
    ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG
    GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA
    GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA
    GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC
    CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA
    GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA
    AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG
    ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT
    GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC
    CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35672
    Clone ID Related Accession (Same CDS sequence) XM_005228269.4 , XM_005228269.3 , XM_015470503.1
    Accession Version XM_015470503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
    GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT
    CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT
    GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC
    ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT
    TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT
    AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC
    TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC
    ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT
    AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA
    CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT
    TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT
    CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC
    TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC
    TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC
    CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG
    AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC
    ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG
    CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC
    ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA
    GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC
    TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT
    CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA
    AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT
    ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT
    GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG
    CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT
    CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG
    CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG
    CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC
    ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA
    GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT
    CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT
    TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT
    CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC
    AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC
    TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC
    AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT
    GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG
    AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG
    GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC
    ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG
    ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG
    GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC
    ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG
    GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA
    GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA
    GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC
    CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA
    GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA
    AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG
    ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT
    GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC
    CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325989.1
    CDS385..3603
    Translation

    Target ORF information:

    RefSeq Version XM_015470503.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATGAAA TGGTCCAGCC AGCCTGGTTG GGCTTCCTGG GGCCAGTGTT GAGGGCTGAG 
    GTGGGAGATG TCATCCTTAT CCACTTGAAG AATTTTGCCA CTCGTCCCTA TACCATCCAT
    CCCCATGGTG TATTCTATGA AAAGGACTCA GAAGGTTCCT TGTATCCAGA TGGTTCCTCT
    GGTTGGTTGA AAGCTGATGA TTCTGTACCC CCAGGAGGCA GCCACATCTA CAACTGGACC
    ATTCCAGAAG GCCATGCACC CACTGATGCT GACCCAGCAT GCCTCACCTG GGTCTACCAT
    TCTCATGTAG ATGCACCACG AGATATTGCT ACTGGTCTCA TTGGACCCCT TATAACCTGT
    AAAAGAGGAA CCCTGGATGG GAGCTTCCCT CCTCGGCGGC AGGATGTGGA CAATGATTTC
    TTCCTCCTTT TTAGTGTGAT AGATGAGAAC CTTAGCTGGC ATATCAATGA GAACATTGCC
    ACTTACTGCT CAGACCCTGC CTCAGTGGAC AAAGAAGATG AGGACTTTCA GAAGAGCAAT
    AGGATGCACG CAATAAATGG CTTTGTATTT GGAAACTTGC CAGATCTAAG TATGTGTGCA
    CAGAAACGTG TGGCCTGGCA CTTGTTTGGC ATGGGCAATG AGGTAGATGT CCACACAGTT
    TTCTTCCATG GACAGATGCT GACCATCCGA GGTCATAGAA CTGATGTGGC TCATATCTTT
    CCAGCCACTT TTGTGAGTGC TGAGATGGTG CCCCAGGAAC CTGGCACCTG GTTAATCAGC
    TGCCAAGTGA ATAGTCATTT GCAAGATGGC ATGCAAGCAC TCTACAAGGT CAAGTCTTGC
    TCCATGGCAC CTCCCATGAA CTTACTCACA GGCAAAGTCC GGCAGTACTT CATTGAGGCC
    CATGAGATTC AATGGGACTA TGGCCCTATG GGGCATGATG GGAGTACGGG AAAGAACTTG
    AGGGAGCCAG GCAGTGGCTC AGATAAATTC TTCCAGAAGA GCTCCATCCG AATTGGGGGC
    ACTTACTGGA AAGTTCGATA TGAAGCCTTC CAAGATGAGA CATTCCAGGA AAAGGTGCAG
    CTGGAGGAGG ATAAGCATCT TGGAATTCTG GGACCAGTGA TCCGGGCTGA GGTGGGTGAC
    ACCATCCTGG TGATCTTCTA CAACCGTGCC TCCCAGCCAT TCAGCATACA GCCTCATGGA
    GTCTTTTATG AGAAGGACTC TGAGGGAACT GTGTACAATG ATGACTCTTC CCACTCTGGC
    TTAGTGGCTA GGCCCTTTGA GAAAGTAACA TACCGCTGGA CAGTCCCTCC CCATGCTGGT
    CCCACTGTTC AGGATCCTGC CTGTCTCACC TGGACATACT TCTCTGCTGC AGATCCCATA
    AGGGATACCA ATTCTGGTCT GGTGGGCCCC CTGCTGGTGT GCAAGGCTGG CACCTTGGGT
    ACAGATGGCA AGCAGAAAGG GGTGGATAAA GAATTCTTTC TCCTTTTCAC TGTGTTGGAT
    GAGAACAAGA GCTGGTACAG CAATGCCAAT CAAGCAGCTG CTATGTTGGA TTCCCGACTG
    CTCTCAGAGG ATGCCAAGGG TTTTCAAGAC TCTAATCGGA TGCATGCCAT TAATGGGTTT
    CTGTTCTCTA ATCTGCCCAA GCTGGACATG TGCAAGGGTG ACACAGTGGC CTGGCACCTG
    CTTGGCCTGG GCACAGAGAC TGATGTGCAC GGGGTCACAT TCCAGGGCAA CACTGTGCAG
    CTTCAGGGCA TGAGGAAGAG TGCAGCCACG CTCTTTCCTC ACACCTTTGT TATGGCCATC
    ATGCAGCCTG ACAACCCTGG GACATTTGAA ATTTACTGCC AAGCAGGCAG CCATAGAGAA
    GCAGGGATGA GGGCAATCTA TAATGTCTCC CTTTGTCCTG GACACCAAGC CAATCCTCAT
    CAACGTTACC AAGCTGCAAG GATCTACTAC ATCATGGCAG AAGAGATGGA GTGGGACTAT
    TGCCCAGACA GGAGCTGGGA ACTAGAATGG CACAACCAGT CTGAGAAGGA CAGTTATGGT
    CACATCTTTC TGAGCAACAA AGATGGACTC CTGGGTTCAA GATACAAGAA AGCTGTATTC
    AGAGAGTATA CTGATGGCAC ATTCAGGGTC CAACGGCCAA GGACTGGATC AGAGGAACAC
    TTGGGAATCT TAGGTCCACT TATCAGAGGA GAGGTTGGTG ATATTCTAAC TGTGGTGTTC
    AAGAACAATG CCAGCCGCCC CTACTCTGTG CATGCCCATG GAGTGCTAGA ATCCAGCACT
    GGTTGGCCAC TGGCTGCTGA GCCTGGTGAG GTTCTCACTT ACCAATGGAA CATTCCAGAG
    AGATCTGGCC CTGGTCTCAA TGATTCTGCT TGTGTTTCTT GGATCTACTA TTCTGCAGTG
    GATCCCATCA AGGACATGTA CAGTGGCCTG GTGGGACCCT TGGCCATCTG CCGAAAAGGC
    ATCCTGGAGC CCTACGGAGG ACGACGTGAC ATGAACCGAG AATTTGCCTT GTTATTCTTG
    ATTTTTGATG AGAACAAGTC TTGGTATCTG GAGGAGAATG TGGAAACTTA TGGGTCCCAG
    GATCCAGGCC GTGTTAACCT ACAGGATGAG ACTTTCTTGG AGAGCAATAA AATGCATGCC
    ATTAATGGAA AGCTCTATGC CAACCTCATG GGCCTTACAA TGTACCAAGG AGAACGAGTG
    GCCTGGTACA TGATGGCAAT GGGACAAGAT ATTGACCTAC ACACTGTCCA TTTCCATGCA
    GAAAGCTTTC TCTACCGGAG TGGAGAGAGC TACCGGGCAG ATGTAGTGGA TTTGTTTCCA
    GGGACCTTTG AGGTTGTGGA GATGGTGGCC AGCAACCCTG GGACATGGCT GCTTCACTGC
    CATGTTACTG ACCATGTCCA TGCTGGCATG GAGACAGTCT TTACGGTCTT GTCCCAAAGA
    GAACACTTAA GCACTACCAC CACCATCCCT GAAGAGATTG GAAAAGTAGC AATTCCCAGA
    AACATTGGAG AAGGCAGTGT GAAGATGCTG GGCATGCAAA TCCCTATAAA GGATATTGAG
    ATGCTGACTT CTGTCTTGAT TGCCATGGGT GTCATTCTCC TGCTCATTGC TCTGGTTCTT
    GGAGGTGTGG TGTGGTACCA GCATCGTCAG AGGAAGTTAC GGCGCAACAG GAGGTCCATC
    CTGGATGACA GCTTCAAGCT TCTGTCTCTC AAGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47790
    Clone ID Related Accession (Same CDS sequence) XM_010822043.2 , XM_015470502.1 , XM_010822043.3
    Accession Version XM_010822043.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104741.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG
    ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT
    TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA
    GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC
    TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG
    TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA
    ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC
    AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG
    CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT
    GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC
    ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG
    GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT
    GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA
    GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT
    GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG
    TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC
    TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC
    TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT
    ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC
    TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT
    GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG
    ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT
    GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT
    GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820345.1
    CDS265..3597
    Translation

    Target ORF information:

    RefSeq Version XM_010822043.2
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822043.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG
    ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT
    TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA
    GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC
    TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG
    TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA
    ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC
    AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG
    CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT
    GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC
    ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG
    GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT
    GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA
    GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT
    GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG
    TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC
    TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC
    TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT
    ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC
    TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT
    GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG
    ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT
    GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT
    GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47790
    Clone ID Related Accession (Same CDS sequence) XM_010822043.2 , XM_015470502.1 , XM_010822043.3
    Accession Version XM_015470502.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product hephaestin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG
    ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT
    TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA
    GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC
    TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG
    TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA
    ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC
    AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG
    CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT
    GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC
    ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG
    GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT
    GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA
    GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT
    GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG
    TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC
    TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC
    TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT
    ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC
    TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT
    GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG
    ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT
    GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT
    GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325988.1
    CDS265..3597
    Translation

    Target ORF information:

    RefSeq Version XM_015470502.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG
    ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT
    TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA
    GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC
    TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG
    TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA
    ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC
    AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG
    CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT
    GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC
    ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG
    GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT
    GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA
    GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT
    GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG
    TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC
    TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC
    TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT
    ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC
    TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT
    GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG
    ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT
    GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT
    GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47790
    Clone ID Related Accession (Same CDS sequence) XM_010822043.2 , XM_015470502.1 , XM_010822043.3
    Accession Version XM_010822043.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hephaestin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010822043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG
    ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT
    TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA
    GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC
    TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG
    TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA
    ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC
    AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG
    CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT
    GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC
    ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG
    GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT
    GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA
    GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT
    GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG
    TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC
    TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC
    TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT
    ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC
    TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT
    GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG
    ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT
    GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT
    GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820345.1
    CDS268..3600
    Translation

    Target ORF information:

    RefSeq Version XM_010822043.3
    Organism Bos taurus(cattle)
    Definition Bos taurus hephaestin (HEPH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822043.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATATGG AAGGATTTTC TCCAGCAAAG AAGTTATTCA TTTGCCTGTT TCCCAGAGTA 
    ACGTGGGCCA TGGAGTCAGG CCATCTCCTC TGGGCTCTGC TGTTCATGCA ACCTCTGTGG
    CCCCAACTGA CTGATGGGAC TACTAGAGTC TACTACCTGG GGATCCAGGA TGTGCGGTGG
    AACTATGCTC CTAAGGGAAG GAATATCATC ACGAACCAGC CTCTGGAAAG TGACATAGTA
    GCTGCTAGCT TCTTGAAGTC TGACAAGAAC CGAATAGGGA ACAGCTACAA GAAGACCATC
    TACAAGGAAT ACAAGGATGG CTCATACATG GATGAAATGG TCCAGCCAGC CTGGTTGGGC
    TTCCTGGGGC CAGTGTTGAG GGCTGAGGTG GGAGATGTCA TCCTTATCCA CTTGAAGAAT
    TTTGCCACTC GTCCCTATAC CATCCATCCC CATGGTGTAT TCTATGAAAA GGACTCAGAA
    GGTTCCTTGT ATCCAGATGG TTCCTCTGGT TGGTTGAAAG CTGATGATTC TGTACCCCCA
    GGAGGCAGCC ACATCTACAA CTGGACCATT CCAGAAGGCC ATGCACCCAC TGATGCTGAC
    CCAGCATGCC TCACCTGGGT CTACCATTCT CATGTAGATG CACCACGAGA TATTGCTACT
    GGTCTCATTG GACCCCTTAT AACCTGTAAA AGAGGAACCC TGGATGGGAG CTTCCCTCCT
    CGGCGGCAGG ATGTGGACAA TGATTTCTTC CTCCTTTTTA GTGTGATAGA TGAGAACCTT
    AGCTGGCATA TCAATGAGAA CATTGCCACT TACTGCTCAG ACCCTGCCTC AGTGGACAAA
    GAAGATGAGG ACTTTCAGAA GAGCAATAGG ATGCACGCAA TAAATGGCTT TGTATTTGGA
    AACTTGCCAG ATCTAAGTAT GTGTGCACAG AAACGTGTGG CCTGGCACTT GTTTGGCATG
    GGCAATGAGG TAGATGTCCA CACAGTTTTC TTCCATGGAC AGATGCTGAC CATCCGAGGT
    CATAGAACTG ATGTGGCTCA TATCTTTCCA GCCACTTTTG TGAGTGCTGA GATGGTGCCC
    CAGGAACCTG GCACCTGGTT AATCAGCTGC CAAGTGAATA GTCATTTGCA AGATGGCATG
    CAAGCACTCT ACAAGGTCAA GTCTTGCTCC ATGGCACCTC CCATGAACTT ACTCACAGGC
    AAAGTCCGGC AGTACTTCAT TGAGGCCCAT GAGATTCAAT GGGACTATGG CCCTATGGGG
    CATGATGGGA GTACGGGAAA GAACTTGAGG GAGCCAGGCA GTGGCTCAGA TAAATTCTTC
    CAGAAGAGCT CCATCCGAAT TGGGGGCACT TACTGGAAAG TTCGATATGA AGCCTTCCAA
    GATGAGACAT TCCAGGAAAA GGTGCAGCTG GAGGAGGATA AGCATCTTGG AATTCTGGGA
    CCAGTGATCC GGGCTGAGGT GGGTGACACC ATCCTGGTGA TCTTCTACAA CCGTGCCTCC
    CAGCCATTCA GCATACAGCC TCATGGAGTC TTTTATGAGA AGGACTCTGA GGGAACTGTG
    TACAATGATG ACTCTTCCCA CTCTGGCTTA GTGGCTAGGC CCTTTGAGAA AGTAACATAC
    CGCTGGACAG TCCCTCCCCA TGCTGGTCCC ACTGTTCAGG ATCCTGCCTG TCTCACCTGG
    ACATACTTCT CTGCTGCAGA TCCCATAAGG GATACCAATT CTGGTCTGGT GGGCCCCCTG
    CTGGTGTGCA AGGCTGGCAC CTTGGGTACA GATGGCAAGC AGAAAGGGGT GGATAAAGAA
    TTCTTTCTCC TTTTCACTGT GTTGGATGAG AACAAGAGCT GGTACAGCAA TGCCAATCAA
    GCAGCTGCTA TGTTGGATTC CCGACTGCTC TCAGAGGATG CCAAGGGTTT TCAAGACTCT
    AATCGGATGC ATGGGACATT TGAAATTTAC TGCCAAGCAG GCAGCCATAG AGAAGCAGGG
    ATGAGGGCAA TCTATAATGT CTCCCTTTGT CCTGGACACC AAGCCAATCC TCATCAACGT
    TACCAAGCTG CAAGGATCTA CTACATCATG GCAGAAGAGA TGGAGTGGGA CTATTGCCCA
    GACAGGAGCT GGGAACTAGA ATGGCACAAC CAGTCTGAGA AGGACAGTTA TGGTCACATC
    TTTCTGAGCA ACAAAGATGG ACTCCTGGGT TCAAGATACA AGAAAGCTGT ATTCAGAGAG
    TATACTGATG GCACATTCAG GGTCCAACGG CCAAGGACTG GATCAGAGGA ACACTTGGGA
    ATCTTAGGTC CACTTATCAG AGGAGAGGTT GGTGATATTC TAACTGTGGT GTTCAAGAAC
    AATGCCAGCC GCCCCTACTC TGTGCATGCC CATGGAGTGC TAGAATCCAG CACTGGTTGG
    CCACTGGCTG CTGAGCCTGG TGAGGTTCTC ACTTACCAAT GGAACATTCC AGAGAGATCT
    GGCCCTGGTC TCAATGATTC TGCTTGTGTT TCTTGGATCT ACTATTCTGC AGTGGATCCC
    ATCAAGGACA TGTACAGTGG CCTGGTGGGA CCCTTGGCCA TCTGCCGAAA AGGCATCCTG
    GAGCCCTACG GAGGACGACG TGACATGAAC CGAGAATTTG CCTTGTTATT CTTGATTTTT
    GATGAGAACA AGTCTTGGTA TCTGGAGGAG AATGTGGAAA CTTATGGGTC CCAGGATCCA
    GGCCGTGTTA ACCTACAGGA TGAGACTTTC TTGGAGAGCA ATAAAATGCA TGCCATTAAT
    GGAAAGCTCT ATGCCAACCT CATGGGCCTT ACAATGTACC AAGGAGAACG AGTGGCCTGG
    TACATGATGG CAATGGGACA AGATATTGAC CTACACACTG TCCATTTCCA TGCAGAAAGC
    TTTCTCTACC GGAGTGGAGA GAGCTACCGG GCAGATGTAG TGGATTTGTT TCCAGGGACC
    TTTGAGGTTG TGGAGATGGT GGCCAGCAAC CCTGGGACAT GGCTGCTTCA CTGCCATGTT
    ACTGACCATG TCCATGCTGG CATGGAGACA GTCTTTACGG TCTTGTCCCA AAGAGAACAC
    TTAAGCACTA CCACCACCAT CCCTGAAGAG ATTGGAAAAG TAGCAATTCC CAGAAACATT
    GGAGAAGGCA GTGTGAAGAT GCTGGGCATG CAAATCCCTA TAAAGGATAT TGAGATGCTG
    ACTTCTGTCT TGATTGCCAT GGGTGTCATT CTCCTGCTCA TTGCTCTGGT TCTTGGAGGT
    GTGGTGTGGT ACCAGCATCG TCAGAGGAAG TTACGGCGCA ACAGGAGGTC CATCCTGGAT
    GACAGCTTCA AGCTTCTGTC TCTCAAGCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.