Heph cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Heph
Entrez Gene ID	117240
Full Name	hephaestin
General protein information	Preferred Names hephaestin Names hephaestin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq22
Summary	may play a role in intestinal iron absorption [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008773248.1	XP_008771470.1	hephaestin isoform X1
NM_133304.1	NP_579838.1	hephaestin precursor
XM_006257046.3	XP_006257108.1	hephaestin isoform X1
XM_006257048.3	XP_006257110.1	hephaestin isoform X1
XM_008773249.2	XP_008771471.1	hephaestin isoform X1
XM_008773250.2	XP_008771472.1	hephaestin isoform X1
XM_006257049.3	XP_006257111.1	hephaestin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEPH	NP_620074.3
Pan troglodytes (chimpanzee)	HEPH	XP_003954062.1
	HEPH	XP_001099571.2
Bos taurus (cattle)	HEPH	XP_005228323.1
Canis lupus familiaris (dog)	HEPH	XP_005641482.1
Mus musculus (house mouse)	Heph	NP_001153100.1
Rattus norvegicus (Norway rat)	Heph	NP_579838.1
Gallus gallus (chicken)	HEPH	XP_420165.4

Gene Ontology
Bibliography

Related articles in PubMed

Ironing out Ferroportin.
Drakesmith H, Nemeth E, Ganz T
Cell metabolism22(5)777-87(2015 Nov)

Age-dependent expression of duodenal cytochrome b, divalent metal transporter 1, ferroportin 1, and hephaestin in the duodenum of rats.
Kong WN, Wu Q, Shen D, Zhao SE, Guo P, Duan XL, Chang YZ
Journal of gastroenterology and hepatology30(3)513-20(2015 Mar)

Functional role of the putative iron ligands in the ferroxidase activity of recombinant human hephaestin.
Vashchenko G, Macgillivray RT
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry17(8)1187-95(2012 Dec)

Immunoreactive hephaestin and ferroxidase activity are present in the cytosolic fraction of rat enterocytes.
Ranganathan PN, Lu Y, Fuqua BK, Collins JF
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine25(4)687-95(2012 Aug)

Interactions between ferroportin and hephaestin in rat enterocytes are reduced after iron ingestion.
Yeh KY, Yeh M, Glass J
Gastroenterology141(1)292-9, 299.e1(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hepcidin or enterocyte iron levels may be involved in the regulation of the age-dependent expression in the duodenum.
Title: Age-dependent expression of duodenal cytochrome b, divalent metal transporter 1, ferroportin 1, and hephaestin in the duodenum of rats.

Cytosolic Heph increased upon iron-deprivation but more important, decreased significantly upon copper-deprivation, mimicking the response of membrane-bound Heph.
Title: Immunoreactive hephaestin and ferroxidase activity are present in the cytosolic fraction of rat enterocytes.

Reduced interactions between Fpn and Heph after iron ingestion indicate that this is a regulatory mechanism for limiting further iron absorption.
Title: Interactions between ferroportin and hephaestin in rat enterocytes are reduced after iron ingestion.

Results suggest that FPN1 and/or HP might directly contribute to iron efflux process from neurons in conditions of overexpression, thus prevent cellular iron accumulation and eventually protect cells from iron-induced oxidative stress.
Title: Ferroportin1 and hephaestin overexpression attenuate iron-induced oxidative stress in MES23.5 dopaminergic cells.

Iron feeding induces ferroportin 1 and hephaestin migration and interaction in rat duodenal epithelium.
Title: Iron feeding induces ferroportin 1 and hephaestin migration and interaction in rat duodenal epithelium.

The following Heph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Heph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00575	NM_133304.1 Latest version!	Rattus norvegicus hephaestin (Heph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa00575	XM_006257049.3 Latest version!	Rattus norvegicus hephaestin (Heph), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa00575	XM_008773249.2 Latest version!	Rattus norvegicus hephaestin (Heph), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa00575	XM_008773248.1 Latest version!	Rattus norvegicus hephaestin (Heph), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa00575	XM_008773250.2 Latest version!	Rattus norvegicus hephaestin (Heph), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa00575	XM_006257046.3 Latest version!	Rattus norvegicus hephaestin (Heph), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa00575	XM_006257048.3 Latest version!	Rattus norvegicus hephaestin (Heph), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Heph ( NM_133304.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_579838.1 Rattus norvegicus hephaestin (Heph), mRNA.

Price & Availability↑

CloneID	ORa00575
Clone ID Related Accession (Same CDS sequence)	XM_008773248.1 , NM_133304.1 , XM_006257046.3 , XM_006257048.3 , XM_008773249.2 , XM_008773250.2 , XM_006257049.3
Accession Version	NM_133304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	hephaestin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF246120.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF246120.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579838.1
CDS	82..3555
Translation	MKAGHLLWALLLMHSLCSLPTDGAIRNYYLGIQDIQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGGTYKKTVYKEYSDGTYTNEIAKPAWLGFLGPLLKAEMGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPEGHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGPFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQTLSIRGHRTDVAHIFPATFVTAEMVPQKSGTWLISCVVNSHLKSGMQAFYKVDSCSMDPPVEQLTGKVRQYFIQAHEIQWDYGPIGHDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERLHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKSSEGTVYNDGTSYPKVAKSFEKVTYYWTVPPHAGPTAEDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGEDGKQKGVDKEFFLLFTIFDENESWYNNANQAAGMLDSRLLSEDVEGFEDSNRMHAINGFLFSNLPRLDICKGDTVAWHLLGLGTENDVHGVMFEGNTLQLQGMRKSAAMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREAGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWYNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPQPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESSTGWPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQETSHVNLQDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGHSYRADVVDLFPGTFEVVEMVASNPGAWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILQNIGEGNVKMLGMNIPVKNVEILSSALIAICVVLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	NM_133304.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hephaestin (Heph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133304.1

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( XM_008773248.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771470.1 Rattus norvegicus hephaestin (Heph), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa00575
Clone ID Related Accession (Same CDS sequence)	XM_008773248.1 , NM_133304.1 , XM_006257046.3 , XM_006257048.3 , XM_008773249.2 , XM_008773250.2 , XM_006257049.3
Accession Version	XM_008773248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771470.1
CDS	1015..4488
Translation	MKAGHLLWALLLMHSLCSLPTDGAIRNYYLGIQDIQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGGTYKKTVYKEYSDGTYTNEIAKPAWLGFLGPLLKAEMGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPEGHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGPFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQTLSIRGHRTDVAHIFPATFVTAEMVPQKSGTWLISCVVNSHLKSGMQAFYKVDSCSMDPPVEQLTGKVRQYFIQAHEIQWDYGPIGHDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERLHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKSSEGTVYNDGTSYPKVAKSFEKVTYYWTVPPHAGPTAEDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGEDGKQKGVDKEFFLLFTIFDENESWYNNANQAAGMLDSRLLSEDVEGFEDSNRMHAINGFLFSNLPRLDICKGDTVAWHLLGLGTENDVHGVMFEGNTLQLQGMRKSAAMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREAGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWYNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPQPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESSTGWPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQETSHVNLQDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGHSYRADVVDLFPGTFEVVEMVASNPGAWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILQNIGEGNVKMLGMNIPVKNVEILSSALIAICVVLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_008773248.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hephaestin (Heph), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773248.1

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( XM_006257046.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257108.1 Rattus norvegicus hephaestin (Heph), transcript variant X1, mRNA.

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CloneID	ORa00575
Clone ID Related Accession (Same CDS sequence)	XM_008773248.1 , NM_133304.1 , XM_006257046.3 , XM_006257048.3 , XM_008773249.2 , XM_008773250.2 , XM_006257049.3
Accession Version	XM_006257046.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257108.1
CDS	459..3932
Translation	MKAGHLLWALLLMHSLCSLPTDGAIRNYYLGIQDIQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGGTYKKTVYKEYSDGTYTNEIAKPAWLGFLGPLLKAEMGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPEGHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGPFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQTLSIRGHRTDVAHIFPATFVTAEMVPQKSGTWLISCVVNSHLKSGMQAFYKVDSCSMDPPVEQLTGKVRQYFIQAHEIQWDYGPIGHDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERLHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKSSEGTVYNDGTSYPKVAKSFEKVTYYWTVPPHAGPTAEDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGEDGKQKGVDKEFFLLFTIFDENESWYNNANQAAGMLDSRLLSEDVEGFEDSNRMHAINGFLFSNLPRLDICKGDTVAWHLLGLGTENDVHGVMFEGNTLQLQGMRKSAAMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREAGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWYNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPQPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESSTGWPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQETSHVNLQDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGHSYRADVVDLFPGTFEVVEMVASNPGAWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILQNIGEGNVKMLGMNIPVKNVEILSSALIAICVVLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_006257046.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hephaestin (Heph), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257046.3

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( XM_006257048.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257110.1 Rattus norvegicus hephaestin (Heph), transcript variant X5, mRNA.

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CloneID	ORa00575
Clone ID Related Accession (Same CDS sequence)	XM_008773248.1 , NM_133304.1 , XM_006257046.3 , XM_006257048.3 , XM_008773249.2 , XM_008773250.2 , XM_006257049.3
Accession Version	XM_006257048.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257110.1
CDS	598..4071
Translation	MKAGHLLWALLLMHSLCSLPTDGAIRNYYLGIQDIQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGGTYKKTVYKEYSDGTYTNEIAKPAWLGFLGPLLKAEMGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPEGHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGPFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQTLSIRGHRTDVAHIFPATFVTAEMVPQKSGTWLISCVVNSHLKSGMQAFYKVDSCSMDPPVEQLTGKVRQYFIQAHEIQWDYGPIGHDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERLHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKSSEGTVYNDGTSYPKVAKSFEKVTYYWTVPPHAGPTAEDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGEDGKQKGVDKEFFLLFTIFDENESWYNNANQAAGMLDSRLLSEDVEGFEDSNRMHAINGFLFSNLPRLDICKGDTVAWHLLGLGTENDVHGVMFEGNTLQLQGMRKSAAMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREAGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWYNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPQPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESSTGWPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQETSHVNLQDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGHSYRADVVDLFPGTFEVVEMVASNPGAWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILQNIGEGNVKMLGMNIPVKNVEILSSALIAICVVLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_006257048.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hephaestin (Heph), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257048.3

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( XM_008773249.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771471.1 Rattus norvegicus hephaestin (Heph), transcript variant X3, mRNA.

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CloneID	ORa00575
Clone ID Related Accession (Same CDS sequence)	XM_008773248.1 , NM_133304.1 , XM_006257046.3 , XM_006257048.3 , XM_008773249.2 , XM_008773250.2 , XM_006257049.3
Accession Version	XM_008773249.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771471.1
CDS	454..3927
Translation	MKAGHLLWALLLMHSLCSLPTDGAIRNYYLGIQDIQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGGTYKKTVYKEYSDGTYTNEIAKPAWLGFLGPLLKAEMGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPEGHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGPFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQTLSIRGHRTDVAHIFPATFVTAEMVPQKSGTWLISCVVNSHLKSGMQAFYKVDSCSMDPPVEQLTGKVRQYFIQAHEIQWDYGPIGHDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERLHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKSSEGTVYNDGTSYPKVAKSFEKVTYYWTVPPHAGPTAEDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGEDGKQKGVDKEFFLLFTIFDENESWYNNANQAAGMLDSRLLSEDVEGFEDSNRMHAINGFLFSNLPRLDICKGDTVAWHLLGLGTENDVHGVMFEGNTLQLQGMRKSAAMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREAGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWYNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPQPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESSTGWPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQETSHVNLQDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGHSYRADVVDLFPGTFEVVEMVASNPGAWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILQNIGEGNVKMLGMNIPVKNVEILSSALIAICVVLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_008773249.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hephaestin (Heph), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773249.2

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( XM_008773250.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771472.1 Rattus norvegicus hephaestin (Heph), transcript variant X4, mRNA.

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CloneID	ORa00575
Clone ID Related Accession (Same CDS sequence)	XM_008773248.1 , NM_133304.1 , XM_006257046.3 , XM_006257048.3 , XM_008773249.2 , XM_008773250.2 , XM_006257049.3
Accession Version	XM_008773250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771472.1
CDS	462..3935
Translation	MKAGHLLWALLLMHSLCSLPTDGAIRNYYLGIQDIQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGGTYKKTVYKEYSDGTYTNEIAKPAWLGFLGPLLKAEMGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPEGHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGPFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQTLSIRGHRTDVAHIFPATFVTAEMVPQKSGTWLISCVVNSHLKSGMQAFYKVDSCSMDPPVEQLTGKVRQYFIQAHEIQWDYGPIGHDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERLHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKSSEGTVYNDGTSYPKVAKSFEKVTYYWTVPPHAGPTAEDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGEDGKQKGVDKEFFLLFTIFDENESWYNNANQAAGMLDSRLLSEDVEGFEDSNRMHAINGFLFSNLPRLDICKGDTVAWHLLGLGTENDVHGVMFEGNTLQLQGMRKSAAMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREAGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWYNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPQPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESSTGWPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQETSHVNLQDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGHSYRADVVDLFPGTFEVVEMVASNPGAWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILQNIGEGNVKMLGMNIPVKNVEILSSALIAICVVLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_008773250.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hephaestin (Heph), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773250.2

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( XM_006257049.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257111.1 Rattus norvegicus hephaestin (Heph), transcript variant X6, mRNA.

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CloneID	ORa00575
Clone ID Related Accession (Same CDS sequence)	XM_008773248.1 , NM_133304.1 , XM_006257046.3 , XM_006257048.3 , XM_008773249.2 , XM_008773250.2 , XM_006257049.3
Accession Version	XM_006257049.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hephaestin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257111.1
CDS	398..3871
Translation	MKAGHLLWALLLMHSLCSLPTDGAIRNYYLGIQDIQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGGTYKKTVYKEYSDGTYTNEIAKPAWLGFLGPLLKAEMGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPEGHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGPFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQTLSIRGHRTDVAHIFPATFVTAEMVPQKSGTWLISCVVNSHLKSGMQAFYKVDSCSMDPPVEQLTGKVRQYFIQAHEIQWDYGPIGHDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERLHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKSSEGTVYNDGTSYPKVAKSFEKVTYYWTVPPHAGPTAEDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGEDGKQKGVDKEFFLLFTIFDENESWYNNANQAAGMLDSRLLSEDVEGFEDSNRMHAINGFLFSNLPRLDICKGDTVAWHLLGLGTENDVHGVMFEGNTLQLQGMRKSAAMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREAGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWYNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPQPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESSTGWPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQETSHVNLQDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGHSYRADVVDLFPGTFEVVEMVASNPGAWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILQNIGEGNVKMLGMNIPVKNVEILSSALIAICVVLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	XM_006257049.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hephaestin (Heph), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257049.3

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG TTCTCTACCA 
ACTGACGGGG CCATTCGAAA CTACTACCTG GGCATCCAGG ATATACAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTGAACA ATGACACAGT GGCTTCCAGC 
TTCCTAAAGT CTGGCAAAAA CAGGATAGGG GGTACTTACA AGAAGACTGT CTATAAGGAA 
TACAGTGATG GCACATACAC CAATGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAA AGGCTGAGAT GGGGGATGTC ATCCTGATCC ACCTGAAGAA TTTTGCCAGC 
CGGCCTTATA CCATTCACCC TCACGGTGTT TTCTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GATCTTCTGG TTATCTGAAA GCTGATGATT CTGTTCCCCC TGGGGGAAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAGGT CATGCACCCA CTGAAGCAGA CCCAGCCTGC 
CTCACCTGGA TTTACCACTC TCATGTAGAT GCTCCACGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACT CTGGATGGTA ACTCCCCACC TCAAAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CAGTGGACAA AGAAGATGGA 
CCATTTCAAG ACAGCAATAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCC 
GAGTTGAGCA TGTGTGCGCA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAG 
ATAGATGTCC ATACAGCTTT CTTCCACGGA CAGACACTGA GTATCCGTGG ACACCGCACT 
GATGTTGCTC ACATTTTTCC AGCCACCTTT GTGACTGCTG AGATGGTGCC CCAGAAGTCT 
GGAACCTGGT TAATTAGCTG TGTGGTGAAC AGTCACTTGA AAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ATTCTTGTTC CATGGACCCA CCCGTGGAGC AGCTCACAGG CAAAGTTCGT 
CAGTACTTCA TTCAAGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GCATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAGA GGTTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAAC CCCATGGCGT CTTTTATGAG AAAAGCTCTG AGGGCACCGT GTACAATGAT 
GGTACTTCTT ATCCCAAAGT TGCCAAGTCG TTTGAAAAAG TAACATACTA CTGGACAGTT 
CCTCCCCATG CTGGGCCCAC CGCTGAGGAT CCTGCCTGTC TGACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTGCT GGTGTGCAAG 
GCTGGGGCCT TGGGTGAAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTATAT TTGATGAGAA CGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCG AAGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATATGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGAATGATG TACATGGGGT TATGTTTGAG 
GGCAACACTC TACAGCTTCA GGGCATGAGG AAAAGTGCAG CCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGGATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACA GAGAAGCAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCCAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCG TCAAGAGTCT ACTATATCAT GGCAGAGGAG 
ATAGAATGGG ATTATTGCCC TGACAGAAGC TGGGAGCTGG AATGGTATAA CACGTCTGAG 
AAGGACAGCT ATGGCCATGT TTTCTTGAGC AACAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCGG TATTCAGAGA ATACACTGAT GGTACATTCA GAATACCTCA ACCGAGGTCT 
GGACCAGAGG AGCACTTAGG AATTCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTGG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTC 
CTAGAATCTA GTACTGGTTG GCCACAAGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATTC CAGAAAGATC TGGTCCTGGT CCCAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATAAAGGAC ATGTATAGTG GCCTGGTAGG ACCCTTAGTC 
ATCTGCCGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAAAAC CAATCATGGT ATCTGAAAGA GAATATTGCA 
ACATATGGAC CCCAAGAGAC AAGTCATGTT AACTTGCAGG ATGCAACTTT CCTGGAGAGC 
AATAAAATGC ATGCCATCAA TGGGAAACTC TATGCTAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCGTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTGCACTTTC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC ACAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGAGCA 
TGGCTGATGC ACTGCCACGT TACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACT 
GTGTTGTCTC ATGAAGAACA TTTCAGTACT ATGACCACCA TTACTAAAGA GATTGGAAAA 
GCTGTGATTC TGCAGAACAT TGGAGAAGGC AATGTGAAGA TGCTGGGCAT GAATATTCCC 
GTAAAGAACG TTGAGATTTT GTCTTCTGCT TTGATTGCCA TATGTGTGGT TCTACTGCTC 
ATTGCTCTGG CCCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGTCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTGT CTCTCAAGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ironing out Ferroportin.
Cell metabolism22(5)777-87(2015 Nov)
Drakesmith H,Nemeth E,Ganz T

Age-dependent expression of duodenal cytochrome b, divalent metal transporter 1, ferroportin 1, and hephaestin in the duodenum of rats.
Journal of gastroenterology and hepatology30(3)513-20(2015 Mar)
Kong WN,Wu Q,Shen D,Zhao SE,Guo P,Duan XL,Chang YZ

Functional role of the putative iron ligands in the ferroxidase activity of recombinant human hephaestin.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry17(8)1187-95(2012 Dec)
Vashchenko G,Macgillivray RT

Immunoreactive hephaestin and ferroxidase activity are present in the cytosolic fraction of rat enterocytes.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine25(4)687-95(2012 Aug)
Ranganathan PN,Lu Y,Fuqua BK,Collins JF

Interactions between ferroportin and hephaestin in rat enterocytes are reduced after iron ingestion.
Gastroenterology141(1)292-9, 299.e1(2011 Jul)
Yeh KY,Yeh M,Glass J

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Heph cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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