Heph cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Heph
Entrez Gene ID	15203
Full Name	hephaestin
Synonyms	C130006F04Rik,Cpl,mKIAA0698,sla
General protein information	Preferred Names hephaestin Names hephaestin haphaestin sex linked anemia
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X C3\|X 42.69 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_181273.4	NP_851790.1	hephaestin isoform 2 precursor
NM_001159628.1	NP_001153100.1	hephaestin isoform 3 precursor
NM_010417.2	NP_034547.2	hephaestin isoform 1 precursor
NM_001159627.1	NP_001153099.1	hephaestin isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEPH	NP_620074.3
Pan troglodytes (chimpanzee)	HEPH	XP_003954062.1
	HEPH	XP_001099571.2
Bos taurus (cattle)	HEPH	XP_005228323.1
Canis lupus familiaris (dog)	HEPH	XP_005641482.1
Mus musculus (house mouse)	Heph	NP_001153100.1
Rattus norvegicus (Norway rat)	Heph	NP_579838.1
Gallus gallus (chicken)	HEPH	XP_420165.4

Gene Ontology
Bibliography

Related articles in PubMed

Ablation of Hephaestin and Ceruloplasmin Results in Iron Accumulation in Adipocytes and Type 2 Diabetes.
Zheng J, Chen M, Liu G, Xu E, Chen H
FEBS letters(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In mice with mutation of Cp and Heph, iron accumulates in glia, while neurons have low iron levels. Both neurons and glia degenerate and mice become ataxic unless given an iron chelator.
Title: Cp/Heph mutant mice have iron-induced neurodegeneration diminished by deferiprone.

Data (including data from studies in knockout mice) suggest that hephaestin and ceruloplasmin play distinct roles in regulation of gene expression in various regions of the brain and are involved in iron homeostasis.
Title: Hephaestin and ceruloplasmin play distinct but interrelated roles in iron homeostasis in mouse brain.

The multicopper ferroxidase hephaestin enhances intestinal iron absorption in mice.
Title: The multicopper ferroxidase hephaestin enhances intestinal iron absorption in mice.

Cp and Heph are necessary for iron export from the retina but are not essential for iron import into the retina.
Title: Ferroxidase hephaestin's cell-autonomous role in the retinal pigment epithelium.

results show that Heph is expressed by oligodendrocytes and plays an important role in iron efflux from these cells.
Title: Iron efflux from oligodendrocytes is differentially regulated in gray and white matter.

The following Heph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Heph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07224	NM_181273.4 Latest version!	Mus musculus hephaestin (Heph), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00
OMu06138	NM_001159627.1 Latest version!	Mus musculus hephaestin (Heph), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu06138	NM_010417.2 Latest version!	Mus musculus hephaestin (Heph), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu07655	NM_001159628.1 Latest version!	Mus musculus hephaestin (Heph), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Heph ( NM_181273.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_851790.1 Mus musculus hephaestin (Heph), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07224
Clone ID Related Accession (Same CDS sequence)	NM_181273.4
Accession Version	NM_181273.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	hephaestin isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ053588.1 and AK081330.1. On Apr 25, 2009 this sequence version replaced NM_181273.3. Transcript Variant: This variant (2) represents use of an alternate promoter and uses an alternate 3' splice pattern, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK081330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT 
TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA 
TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC 
CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC 
CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA 
GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA 
GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA 
ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT 
GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT 
GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT 
CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT 
GATCCCACAA GAGATACAAA TTCTGGCCTG GTGGGCCCTC TACTGGTGTG CAAGGCTGGG 
GCCTTGGGTG CAGATGGCAA GCAGAAAGGA GTGGATAAAG AATTTTTTCT CCTCTTCACT 
GTGTTTGATG AGAATGAGAG CTGGTACAAC AATGCCAATC AGGCAGCTGG TATGTTGGAT 
TCCCGACTGC TCTCAGAGGA TGTCGAGGGC TTCCAGGACT CCAATCGAAT GCATGCTATT 
AATGGATTTC TGTTCTCTAA CCTGCCCAGG CTGGACATGT GCAAGGGTGA TACTGTGGCC 
TGGCACCTGC TTGGCCTGGG CACAGAGACT GATGTACATG GGGTAATGTT CGAGGGCAAC 
ACTGTGCAGC TTCAGGGCAT GAGGAAAGGT GCAGTCATGC TCTTTCCTCA CACCTTTGTG 
ACGGCCATCA TGCAGCCTGA CAATCCTGGA ATATTTGAAA TCTACTGCCA AGCAGGCAGC 
CACCGAGAGG AAGGGATGCA GGCAATTTAT AATGTCTCTC AGTGTTCTAG TCATCAAGAC 
AGCCCACGCC AACACTACCA AGCTTCAAGA GTCTACTATA TCATGGCAGA AGAGATAGAG 
TGGGATTACT GCCCTGATAG AAGCTGGGAA CTGGAATGGC ATAACACATC TGAGAAAGAC 
AGCTATGGCC ATGTTTTCCT GAGCAATAAA GATGGGCTCC TGGGTTCCAA ATATAAGAAA 
GCTGTATTCA GGGAATACAC TGATGGTACT TTCAGAATAC CTCGGCCAAG GTCTGGACCA 
GAGGAGCACT TGGGAATCCT GGGTCCACTT ATCAGAGGAG AGGTTGGTGA TATCTTGACT 
GTAGTGTTCA AGAATAAGGC CAGTCGACCA TATTCTATAC ATGCCCATGG AGTTCTAGAA 
TCTAACACTG GCGGGCCACA GGCTGCTGAG CCTGGTGAGT TGGAGCACTT AATGAAGAGA 
CAAAGGCTTT ACAATCCTTT TACTTTAGTC TTCTGGTTTA TTCCTTTCAA CATATTACAT 
TCTTGGGCTG GGCAGGTCAC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851790.1
CDS	500..3043
Translation	MKAGHLLWALLLMHSLWSIPTDGAIRNYYLGIQDMQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGSSYKKTVYKEYSDGTYTEEIAKPAWLGFLGPLLQAEVGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPESHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGAFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQMLSIRGHHTDVANIFPATFVTAEMVPQKSGTWLISCEVNSHLRSGMQAFYKVDSCSMDPPVDQLTGKVRQYFIQAHEIQWDYGPIGYDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERVHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKNSEGTVYNDDPTRDTNSGLVGPLLVCKAGALGADGKQKGVDKEFFLLFTVFDENESWYNNANQAAGMLDSRLLSEDVEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFEGNTVQLQGMRKGAVMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREEGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWHNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPRPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESNTGGPQAAEPGELEHLMKRQRLYNPFTLVFWFIPFNILHSWAGQVT

Target ORF information:

RefSeq Version	NM_181273.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hephaestin (Heph), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181273.4

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT 
TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA 
TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC 
CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC 
CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA 
GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA 
GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA 
ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT 
GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT 
GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT 
CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT 
GATCCCACAA GAGATACAAA TTCTGGCCTG GTGGGCCCTC TACTGGTGTG CAAGGCTGGG 
GCCTTGGGTG CAGATGGCAA GCAGAAAGGA GTGGATAAAG AATTTTTTCT CCTCTTCACT 
GTGTTTGATG AGAATGAGAG CTGGTACAAC AATGCCAATC AGGCAGCTGG TATGTTGGAT 
TCCCGACTGC TCTCAGAGGA TGTCGAGGGC TTCCAGGACT CCAATCGAAT GCATGCTATT 
AATGGATTTC TGTTCTCTAA CCTGCCCAGG CTGGACATGT GCAAGGGTGA TACTGTGGCC 
TGGCACCTGC TTGGCCTGGG CACAGAGACT GATGTACATG GGGTAATGTT CGAGGGCAAC 
ACTGTGCAGC TTCAGGGCAT GAGGAAAGGT GCAGTCATGC TCTTTCCTCA CACCTTTGTG 
ACGGCCATCA TGCAGCCTGA CAATCCTGGA ATATTTGAAA TCTACTGCCA AGCAGGCAGC 
CACCGAGAGG AAGGGATGCA GGCAATTTAT AATGTCTCTC AGTGTTCTAG TCATCAAGAC 
AGCCCACGCC AACACTACCA AGCTTCAAGA GTCTACTATA TCATGGCAGA AGAGATAGAG 
TGGGATTACT GCCCTGATAG AAGCTGGGAA CTGGAATGGC ATAACACATC TGAGAAAGAC 
AGCTATGGCC ATGTTTTCCT GAGCAATAAA GATGGGCTCC TGGGTTCCAA ATATAAGAAA 
GCTGTATTCA GGGAATACAC TGATGGTACT TTCAGAATAC CTCGGCCAAG GTCTGGACCA 
GAGGAGCACT TGGGAATCCT GGGTCCACTT ATCAGAGGAG AGGTTGGTGA TATCTTGACT 
GTAGTGTTCA AGAATAAGGC CAGTCGACCA TATTCTATAC ATGCCCATGG AGTTCTAGAA 
TCTAACACTG GCGGGCCACA GGCTGCTGAG CCTGGTGAGT TGGAGCACTT AATGAAGAGA 
CAAAGGCTTT ACAATCCTTT TACTTTAGTC TTCTGGTTTA TTCCTTTCAA CATATTACAT 
TCTTGGGCTG GGCAGGTCAC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( NM_010417.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034547.2 Mus musculus hephaestin (Heph), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06138
Clone ID Related Accession (Same CDS sequence)	NM_010417.2 , NM_001159627.1
Accession Version	NM_010417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	hephaestin isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ067085.1, AF082567.1 and AK129194.1. On Apr 25, 2009 this sequence version replaced NM_010417.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF082567.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT 
TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA 
TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC 
CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC 
CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA 
GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA 
GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA 
ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT 
GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT 
GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT 
CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT 
GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT 
CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG 
GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG 
GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG 
ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG 
AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT 
GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT 
CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC 
ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA 
ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC 
AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA 
TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG 
GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA 
GCAGTGATTC TAAGGGACAT TGGAGGTGAC AATGTGAAGA TGCTGGGCAT GAACATTCCC 
ATAAAGGATG TAGAGATTCT GTCTTCTGCT TTGATTGCCA TATGTGTGCT TCTGTTGCTC 
ATTGCTCTGG CTCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGGCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTCT CTCTCAAGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034547.2
CDS	508..3981
Translation	MKAGHLLWALLLMHSLWSIPTDGAIRNYYLGIQDMQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGSSYKKTVYKEYSDGTYTEEIAKPAWLGFLGPLLQAEVGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPESHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGAFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQMLSIRGHHTDVANIFPATFVTAEMVPQKSGTWLISCEVNSHLRSGMQAFYKVDSCSMDPPVDQLTGKVRQYFIQAHEIQWDYGPIGYDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERVHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKNSEGTVYNDGTSHPKVAKSFEKVTYYWTVPPHAGPTAQDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGADGKQKGVDKEFFLLFTVFDENESWYNNANQAAGMLDSRLLSEDVEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFEGNTVQLQGMRKGAVMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREEGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWHNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPRPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESNTGGPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQESSHVNLKDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGQSYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILRDIGGDNVKMLGMNIPIKDVEILSSALIAICVLLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	NM_010417.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hephaestin (Heph), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010417.2

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT 
TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA 
TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC 
CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC 
CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA 
GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA 
GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA 
ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT 
GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT 
GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT 
CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT 
GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT 
CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG 
GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG 
GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG 
ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG 
AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT 
GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT 
CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC 
ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA 
ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC 
AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA 
TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG 
GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA 
GCAGTGATTC TAAGGGACAT TGGAGGTGAC AATGTGAAGA TGCTGGGCAT GAACATTCCC 
ATAAAGGATG TAGAGATTCT GTCTTCTGCT TTGATTGCCA TATGTGTGCT TCTGTTGCTC 
ATTGCTCTGG CTCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGGCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTCT CTCTCAAGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( NM_001159627.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153099.1 Mus musculus hephaestin (Heph), transcript variant 3, mRNA.

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CloneID	OMu06138
Clone ID Related Accession (Same CDS sequence)	NM_010417.2 , NM_001159627.1
Accession Version	NM_001159627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	hephaestin isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ053588.1, AK129194.1 and AF082567.1. Transcript Variant: This variant (3) represents use of an alternate promoter, compared to variant 1, and enocdes the longest isoform (1). Both variants 1 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129194.1, SRR1660821.102849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT 
TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA 
TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC 
CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC 
CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA 
GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA 
GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA 
ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT 
GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT 
GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT 
CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT 
GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT 
CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG 
GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG 
GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG 
ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG 
AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT 
GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT 
CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC 
ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA 
ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC 
AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA 
TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG 
GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA 
GCAGTGATTC TAAGGGACAT TGGAGGTGAC AATGTGAAGA TGCTGGGCAT GAACATTCCC 
ATAAAGGATG TAGAGATTCT GTCTTCTGCT TTGATTGCCA TATGTGTGCT TCTGTTGCTC 
ATTGCTCTGG CTCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGGCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTCT CTCTCAAGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153099.1
CDS	500..3973
Translation	MKAGHLLWALLLMHSLWSIPTDGAIRNYYLGIQDMQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGSSYKKTVYKEYSDGTYTEEIAKPAWLGFLGPLLQAEVGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPESHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGAFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQMLSIRGHHTDVANIFPATFVTAEMVPQKSGTWLISCEVNSHLRSGMQAFYKVDSCSMDPPVDQLTGKVRQYFIQAHEIQWDYGPIGYDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERVHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKNSEGTVYNDGTSHPKVAKSFEKVTYYWTVPPHAGPTAQDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGADGKQKGVDKEFFLLFTVFDENESWYNNANQAAGMLDSRLLSEDVEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFEGNTVQLQGMRKGAVMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREEGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWHNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPRPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESNTGGPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQESSHVNLKDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGQSYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKAVILRDIGGDNVKMLGMNIPIKDVEILSSALIAICVLLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	NM_001159627.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hephaestin (Heph), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159627.1

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT 
TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA 
TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC 
CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC 
CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA 
GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA 
GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA 
ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT 
GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT 
GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT 
CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT 
GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT 
CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG 
GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG 
GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG 
ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG 
AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT 
GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT 
CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC 
ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA 
ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC 
AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA 
TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG 
GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA 
GCAGTGATTC TAAGGGACAT TGGAGGTGAC AATGTGAAGA TGCTGGGCAT GAACATTCCC 
ATAAAGGATG TAGAGATTCT GTCTTCTGCT TTGATTGCCA TATGTGTGCT TCTGTTGCTC 
ATTGCTCTGG CTCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGGCGC 
AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTCT CTCTCAAGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Heph ( NM_001159628.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153100.1 Mus musculus hephaestin (Heph), transcript variant 4, mRNA.

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CloneID	OMu07655
Clone ID Related Accession (Same CDS sequence)	NM_001159628.1
Accession Version	NM_001159628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	hephaestin isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732365.17. Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting isoform (3) lacks one internal amino acid near the C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT 
TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA 
TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC 
CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC 
CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA 
GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA 
GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA 
ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT 
GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT 
GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT 
CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT 
GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT 
CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG 
GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG 
GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG 
ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG 
AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT 
GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT 
CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC 
ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA 
ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC 
AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA 
TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG 
GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA 
GTGATTCTAA GGGACATTGG AGGTGACAAT GTGAAGATGC TGGGCATGAA CATTCCCATA 
AAGGATGTAG AGATTCTGTC TTCTGCTTTG ATTGCCATAT GTGTGCTTCT GTTGCTCATT 
GCTCTGGCTC TTGGTGGTGT AGTCTGGTAC CAGCATCGAC AAAGAAAGCT TCGGCGCAAC 
AGGAGGTCCA TTCTTGATGA TAGCTTCAAG CTTCTCTCTC TCAAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153100.1
CDS	508..3978
Translation	MKAGHLLWALLLMHSLWSIPTDGAIRNYYLGIQDMQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIGSSYKKTVYKEYSDGTYTEEIAKPAWLGFLGPLLQAEVGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSLYPDGSSGYLKADDSVPPGGSHVYNWSIPESHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGTLDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGAFQDSNRMHAINGFVFGNLPELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQMLSIRGHHTDVANIFPATFVTAEMVPQKSGTWLISCEVNSHLRSGMQAFYKVDSCSMDPPVDQLTGKVRQYFIQAHEIQWDYGPIGYDGRTGKSLREPGSGPDKYFQKSSSRIGGTYWKVRYEAFQDETFQERVHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYEKNSEGTVYNDGTSHPKVAKSFEKVTYYWTVPPHAGPTAQDPACLTWMYFSAADPTRDTNSGLVGPLLVCKAGALGADGKQKGVDKEFFLLFTVFDENESWYNNANQAAGMLDSRLLSEDVEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFEGNTVQLQGMRKGAVMLFPHTFVTAIMQPDNPGIFEIYCQAGSHREEGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWHNTSEKDSYGHVFLSNKDGLLGSKYKKAVFREYTDGTFRIPRPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGVLESNTGGPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPNGGRNDMDREFALLFLIFDENQSWYLKENIATYGPQESSHVNLKDATFLESNKMHAINGKLYANLRGLTVYQGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGQSYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETIFTVLSHEEHFSTMTTITKEIGKVILRDIGGDNVKMLGMNIPIKDVEILSSALIAICVLLLLIALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

Target ORF information:

RefSeq Version	NM_001159628.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hephaestin (Heph), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159628.1

ORF Insert Sequence:

ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT 
CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT 
TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA 
TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA 
CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC 
CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA 
TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC 
CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC 
CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT 
GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG 
GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC 
CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA 
GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA 
GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA 
ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT 
GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT 
GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC 
TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT 
CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC 
AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC 
TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA 
TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA 
AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC 
AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT 
GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT 
CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT 
GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG 
GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC 
TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG 
TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT 
GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT 
GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG 
GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC 
TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA 
GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT 
CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG 
ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG 
AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT 
AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT 
GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC 
TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT 
CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG 
TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT 
TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC 
ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT 
GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA 
ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC 
AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC 
CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT 
GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG 
GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA 
TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG 
GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA 
GTGATTCTAA GGGACATTGG AGGTGACAAT GTGAAGATGC TGGGCATGAA CATTCCCATA 
AAGGATGTAG AGATTCTGTC TTCTGCTTTG ATTGCCATAT GTGTGCTTCT GTTGCTCATT 
GCTCTGGCTC TTGGTGGTGT AGTCTGGTAC CAGCATCGAC AAAGAAAGCT TCGGCGCAAC 
AGGAGGTCCA TTCTTGATGA TAGCTTCAAG CTTCTCTCTC TCAAGCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ablation of Hephaestin and Ceruloplasmin Results in Iron Accumulation in Adipocytes and Type 2 Diabetes.
FEBS letters(2018 Jan)
Zheng J,Chen M,Liu G,Xu E,Chen H

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Heph cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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