RHO cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHO
Entrez Gene ID	509933
Full Name	rhodopsin
General protein information	Preferred Names rhodopsin Names rhodopsin rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 22 Map Location: 22q23-q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014890.2	NP_001014890.1	rhodopsin

Pathways from BioSystems

Homologies

	RHO	XP_001094250.1
Canis lupus familiaris (dog)	RHO	NP_001008277.1
Mus musculus (house mouse)	Rho	NP_663358.1
Xenopus tropicalis (tropical clawed frog)	rho	NP_001090803.1
Homo sapiens (human)	RHO	NP_000530.1
Pan troglodytes (chimpanzee)	RHO	XP_516740.2
Gallus gallus (chicken)	RHO	NP_001025777.1
Bos taurus (cattle)	RHO	NP_001014890.1
Rattus norvegicus (Norway rat)	Rho	NP_254276.1
Danio rerio (zebrafish)	exorh	NP_571287.1

Gene Ontology
Bibliography

Related articles in PubMed

How Rhodopsin Tunes the Equilibrium between Protonated and Deprotonated Forms of the Retinal Chromophore.
van Keulen SC, Solano A, Rothlisberger U
Journal of chemical theory and computation13(9)4524-4534(2017 Sep)

Isolation and structure-function characterization of a signaling-active rhodopsin-G protein complex.
Gao Y, Westfield G, Erickson JW, Cerione RA, Skiniotis G, Ramachandran S
The Journal of biological chemistry292(34)14280-14289(2017 08)

The Energetics of Chromophore Binding in the Visual Photoreceptor Rhodopsin.
Tian H, Sakmar TP, Huber T
Biophysical journal113(1)60-72(2017 Jul)

An experimental comparison of human and bovine rhodopsin provides insight into the molecular basis of retinal disease.
Morrow JM, Castiglione GM, Dungan SZ, Tang PL, Bhattacharyya N, Hauser FE, Chang BSW
FEBS letters591(12)1720-1731(2017 Jun)

Water permeation through the internal water pathway in activated GPCR rhodopsin.
Tomobe K, Yamamoto E, Kholmurodov K, Yasuoka K
PloS one12(5)e0176876(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that a hetero-multimer complex forms between light-activated rhodopsin and light-activated heterotrimeric transducin (T-alpha-1, Gnb1, Gngt1); the stoichiometry is 1:1 rhodopsin:transducin. The complex appears to form on native rod outer segment membranes upon light activation.
Title: Isolation and structure-function characterization of a signaling-active rhodopsin-G protein complex.

Study presents a comprehensive analysis of the kinetics and thermodynamics of the recombination reaction between opsin and 11-cis-retinal (11CR) to form the mature visual pigment, Rho; and found that the lipid bilayer environment is important for ligand binding in Rho.
Title: The Energetics of Chromophore Binding in the Visual Photoreceptor Rhodopsin.

Data suggest that retinitis pigmentosa-associated mutation G51A behaves differently in human rhodopsin compared to bovine rhodopsin; human rhodopsin is more thermally stable than ancestral ancestrally reconstructed mammalian rhodopsin.
Title: An experimental comparison of human and bovine rhodopsin provides insight into the molecular basis of retinal disease.

These findings revealed a total water flux between the bulk and the protein inside in the Meta II state, and suggested that these pathways provide water molecules to the crucial sites of the activated rhodopsin.
Title: Water permeation through the internal water pathway in activated GPCR rhodopsin.

rhodopsin can tolerate a second Lys in the retinal binding pocket and suggest that an evolutionary intermediate with two Lys could allow migration of the Schiff base Lys to a position other than the observed, highly conserved location in the seventh TM helix
Title: Relocating the Active-Site Lysine in Rhodopsin: 2. Evolutionary Intermediates.

The following RHO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa95411	NM_001014890.2 Latest version!	Bos taurus rhodopsin (RHO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHO ( NM_001014890.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001014890.1 Bos taurus rhodopsin (RHO), mRNA.

Price & Availability↑

CloneID	OBa95411
Clone ID Related Accession (Same CDS sequence)	NM_001014890.2
Accession Version	NM_001014890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	rhodopsin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02054571.1. On Apr 15, 2016 this sequence version replaced NM_001014890.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M21606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145448 [ECO:0000348] ##Evidence-Data-END##

ATGAACGGGA CCGAGGGCCC AAACTTCTAC GTGCCTTTCT CCAACAAGAC GGGCGTGGTG 
CGCAGCCCCT TCGAGGCCCC GCAGTACTAC CTGGCGGAGC CATGGCAGTT CTCCATGCTG 
GCCGCCTACA TGTTCCTGCT GATCATGCTT GGCTTCCCCA TCAACTTCCT CACGCTGTAC 
GTCACAGTCC AGCACAAGAA GCTGCGCACA CCCCTCAACT ACATCCTGCT CAACCTGGCC 
GTGGCCGACC TCTTCATGGT CTTCGGGGGC TTCACCACCA CCCTCTACAC CTCTCTGCAC 
GGATACTTCG TCTTTGGGCC CACGGGCTGC AACCTGGAGG GCTTCTTTGC CACCCTGGGC 
GGTGAAATTG CACTGTGGTC CTTGGTGGTC CTGGCCATCG AGCGGTACGT GGTGGTGTGC 
AAGCCCATGA GCAACTTCCG CTTCGGGGAG AACCACGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CTCTGGCCTG TGCCGCGCCC CCCCTCGTCG GCTGGTCCAG GTACATCCCG 
GAGGGCATGC AGTGCTCGTG CGGGATTGAC TACTACACGC CCCACGAGGA GACCAACAAT 
GAGTCGTTCG TCATCTACAT GTTCGTGGTC CACTTCATCA TCCCCCTGAT TGTCATATTC 
TTCTGCTACG GGCAGCTGGT GTTCACCGTC AAGGAGGCGG CTGCCCAGCA GCAGGAGTCG 
GCCACCACTC AGAAGGCCGA GAAGGAGGTC ACCCGCATGG TGATCATCAT GGTCATCGCT 
TTCCTAATCT GCTGGCTGCC CTACGCTGGG GTGGCGTTCT ACATCTTCAC CCATCAGGGC 
TCTGACTTTG GCCCCATCTT CATGACCATC CCGGCTTTCT TTGCCAAGAC TTCTGCCGTC 
TACAACCCCG TCATCTACAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GGTCACCACT 
CTCTGCTGTG GCAAGAACCC GCTGGGTGAC GACGAGGCCT CCACCACCGT CTCCAAGACA 
GAGACCAGCC AGGTGGCGCC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014890.1
CDS	1..1047
Translation	MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA

Target ORF information:

RefSeq Version	NM_001014890.2
Organism	Bos taurus(cattle)
Definition	Bos taurus rhodopsin (RHO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014890.2

ORF Insert Sequence:

ATGAACGGGA CCGAGGGCCC AAACTTCTAC GTGCCTTTCT CCAACAAGAC GGGCGTGGTG 
CGCAGCCCCT TCGAGGCCCC GCAGTACTAC CTGGCGGAGC CATGGCAGTT CTCCATGCTG 
GCCGCCTACA TGTTCCTGCT GATCATGCTT GGCTTCCCCA TCAACTTCCT CACGCTGTAC 
GTCACAGTCC AGCACAAGAA GCTGCGCACA CCCCTCAACT ACATCCTGCT CAACCTGGCC 
GTGGCCGACC TCTTCATGGT CTTCGGGGGC TTCACCACCA CCCTCTACAC CTCTCTGCAC 
GGATACTTCG TCTTTGGGCC CACGGGCTGC AACCTGGAGG GCTTCTTTGC CACCCTGGGC 
GGTGAAATTG CACTGTGGTC CTTGGTGGTC CTGGCCATCG AGCGGTACGT GGTGGTGTGC 
AAGCCCATGA GCAACTTCCG CTTCGGGGAG AACCACGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CTCTGGCCTG TGCCGCGCCC CCCCTCGTCG GCTGGTCCAG GTACATCCCG 
GAGGGCATGC AGTGCTCGTG CGGGATTGAC TACTACACGC CCCACGAGGA GACCAACAAT 
GAGTCGTTCG TCATCTACAT GTTCGTGGTC CACTTCATCA TCCCCCTGAT TGTCATATTC 
TTCTGCTACG GGCAGCTGGT GTTCACCGTC AAGGAGGCGG CTGCCCAGCA GCAGGAGTCG 
GCCACCACTC AGAAGGCCGA GAAGGAGGTC ACCCGCATGG TGATCATCAT GGTCATCGCT 
TTCCTAATCT GCTGGCTGCC CTACGCTGGG GTGGCGTTCT ACATCTTCAC CCATCAGGGC 
TCTGACTTTG GCCCCATCTT CATGACCATC CCGGCTTTCT TTGCCAAGAC TTCTGCCGTC 
TACAACCCCG TCATCTACAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GGTCACCACT 
CTCTGCTGTG GCAAGAACCC GCTGGGTGAC GACGAGGCCT CCACCACCGT CTCCAAGACA 
GAGACCAGCC AGGTGGCGCC TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

How Rhodopsin Tunes the Equilibrium between Protonated and Deprotonated Forms of the Retinal Chromophore.
Journal of chemical theory and computation13(9)4524-4534(2017 Sep)
van Keulen SC,Solano A,Rothlisberger U

Isolation and structure-function characterization of a signaling-active rhodopsin-G protein complex.
The Journal of biological chemistry292(34)14280-14289(2017 08)
Gao Y,Westfield G,Erickson JW,Cerione RA,Skiniotis G,Ramachandran S

The Energetics of Chromophore Binding in the Visual Photoreceptor Rhodopsin.
Biophysical journal113(1)60-72(2017 Jul)
Tian H,Sakmar TP,Huber T

An experimental comparison of human and bovine rhodopsin provides insight into the molecular basis of retinal disease.
FEBS letters591(12)1720-1731(2017 Jun)
Morrow JM,Castiglione GM,Dungan SZ,Tang PL,Bhattacharyya N,Hauser FE,Chang BSW

Water permeation through the internal water pathway in activated GPCR rhodopsin.
PloS one12(5)e0176876(2017)
Tomobe K,Yamamoto E,Kholmurodov K,Yasuoka K

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

RHO cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報