Rho cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rho
Entrez Gene ID	24717
Full Name	rhodopsin
General protein information	Preferred Names rhodopsin Names rhodopsin Rhodopsin (retinitis pigmentosa 4, autosomal dominant)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q42
Summary	visual pigment protein of rod photoreceptors involved in G-protein coupled receptor mediated signaling and phototransduction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_033441.1	NP_254276.1	rhodopsin

Pathways from BioSystems

Homologies

	RHO	XP_001094250.1
Canis lupus familiaris (dog)	RHO	NP_001008277.1
Mus musculus (house mouse)	Rho	NP_663358.1
Xenopus tropicalis (tropical clawed frog)	rho	NP_001090803.1
Homo sapiens (human)	RHO	NP_000530.1
Pan troglodytes (chimpanzee)	RHO	XP_516740.2
Gallus gallus (chicken)	RHO	NP_001025777.1
Bos taurus (cattle)	RHO	NP_001014890.1
Rattus norvegicus (Norway rat)	Rho	NP_254276.1
Danio rerio (zebrafish)	exorh	NP_571287.1

Gene Ontology
Bibliography

Related articles in PubMed

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.
Malechka VV, Moiseyev G, Takahashi Y, Shin Y, Ma JX
The American journal of pathology187(10)2222-2231(2017 Oct)

Allele-Specific Inhibition of Rhodopsin With an Antisense Oligonucleotide Slows Photoreceptor Cell Degeneration.
Murray SF, Jazayeri A, Matthes MT, Yasumura D, Yang H, Peralta R, Watt A, Freier S, Hung G, Adamson PS, Guo S, Monia BP, LaVail MM, McCaleb ML
Investigative ophthalmology & visual science56(11)6362-75(2015 Oct)

Epidermal growth factor induces Ca(2+) sensitization through Rho-kinase-dependent phosphorylation of myosin phosphatase target subunit 1 in vascular smooth muscle.
Sasahara T, Okamoto H, Ohkura N, Kobe A, Yayama K
European journal of pharmacology76289-95(2015 Sep)

Ultrastructural visualization of trans-ciliary rhodopsin cargoes in mammalian rods.
Chuang JZ, Hsu YC, Sung CH
Cilia44(2015)

Photoreceptor degeneration, structural remodeling and glial activation: a morphological study on a genetic mouse model for pericyte deficiency.
Genov? G, Mollick T, Johansson K
Neuroscience279269-84(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results suggest that retinoid metabolism in the eye is impaired in type 1 diabetes, which leads to deficient generation of visual pigments and neural retinal dysfunction in early diabetes.
Title: Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.

Allele-specific ASO-mediated knockdown of mutant P23H rhodopsin expression slowed the rate of photoreceptor degeneration and preserved the function of photoreceptor cells in eyes of the P23H rhodopsin transgenic rat.
Title: Allele-Specific Inhibition of Rhodopsin With an Antisense Oligonucleotide Slows Photoreceptor Cell Degeneration.

EGF induces Ca(2+) sensitization in vascular smooth muscle by Rho-kinase-dependent inactivation of MLCP mediated by the EGF receptor/MEK/Erk1/2 pathway
Title: Epidermal growth factor induces Ca(2+) sensitization through Rho-kinase-dependent phosphorylation of myosin phosphatase target subunit 1 in vascular smooth muscle.

Phosphorylation of the Rho/ROCK signaling target cofilin.
Title: Adiponectin stimulates Rho-mediated actin cytoskeleton remodeling and glucose uptake via APPL1 in primary cardiomyocytes.

We examine and compare the contribution of endoplasmic reticulum stress to retinal degeneration in several vertebrate models of retinitis pigementosa generated through expression of mutant rhodopsins.
Title: Endoplasmic reticulum stress in vertebrate mutant rhodopsin models of retinal degeneration.

The following Rho gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rho cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13158	NM_033441.1 Latest version!	Rattus norvegicus rhodopsin (Rho), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rho ( NM_033441.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_254276.1 Rattus norvegicus rhodopsin (Rho), mRNA.

Price & Availability↑

CloneID	ORa13158
Clone ID Related Accession (Same CDS sequence)	NM_033441.1
Accession Version	NM_033441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	rhodopsin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z46957.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z46957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAACGGCA CAGAGGGCCC CAATTTTTAT GTGCCCTTCT CCAACATCAC GGGCGTGGTG 
CGCAGCCCCT TTGAGCAGCC GCAGTACTAC CTGGCGGAGC CATGGCAGTT CTCCATGCTG 
GCAGCCTACA TGTTCCTGCT CATCGTGCTG GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACCGTAC AGCACAAGAA GCTGCGCACA CCACTCAACT ACATCCTGCT CAACTTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGAGGA TTCACCACCA CCCTCTACAC CTCACTGCAT 
GGCTACTTTG TCTTTGGGCC CACAGGCTGC AACCTTGAGG GCTTCTTTGC CACCCTTGGA 
GGTGAAATCG GCCTGTGGTC CCTGGTAGTC CTGGCCATTG AGCGCTACGT GGTGGTCTGC 
AAGCCCATGA GCAACTTCCG CTTTGGGGAG AATCATGCCA TTATGGGTGT GGCCTTCACC 
TGGGTCATGG CGTTGGCCTG TGCTGCTCCC CCACTGGTTG GCTGGTCCAG GTACATCCCC 
GAGGGCATGC AGTGTTCATG TGGGATTGAC TACTATACAC TCAAGCCTGA GGTCAACAAT 
GAGTCCTTCG TCATCTACAT GTTCGTGGTC CACTTCACCA TCCCCATGAT CGTCATCTTC 
TTCTGCTACG GGCAGCTGGT CTTCACCGTC AAGGAGGCCG CCGCCCAGCA ACAGGAGTCG 
GCTACCACTC AGAAGGCAGA GAAGGAAGTC ACGCGCATGG TCATCATCAT GGTCATCTTC 
TTCCTGATCT GCTGGCTTCC CTATGCCAGT GTGGCCATGT ACATCTTTAC CCACCAGGGC 
TCCAACTTCG GCCCCATCTT CATGACCCTT CCCGCTTTCT TTGCTAAGAC CGCCTCCATC 
TACAACCCAA TCATCTACAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GCTCACCACG 
CTCTGCTGCG GCAAGAATCC ACTGGGAGAT GATGAGGCCT CTGCCACTGC CTCCAAGACG 
GAGACCAGCC AGGTGGCTCC AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_254276.1
CDS	84..1130
Translation	MNGTEGPNFYVPFSNITGVVRSPFEQPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIGLWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIFFLICWLPYASVAMYIFTHQGSNFGPIFMTLPAFFAKTASIYNPIIYIMMNKQFRNCMLTTLCCGKNPLGDDEASATASKTETSQVAPA

Target ORF information:

RefSeq Version	NM_033441.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus rhodopsin (Rho), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033441.1

ORF Insert Sequence:

ATGAACGGCA CAGAGGGCCC CAATTTTTAT GTGCCCTTCT CCAACATCAC GGGCGTGGTG 
CGCAGCCCCT TTGAGCAGCC GCAGTACTAC CTGGCGGAGC CATGGCAGTT CTCCATGCTG 
GCAGCCTACA TGTTCCTGCT CATCGTGCTG GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACCGTAC AGCACAAGAA GCTGCGCACA CCACTCAACT ACATCCTGCT CAACTTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGAGGA TTCACCACCA CCCTCTACAC CTCACTGCAT 
GGCTACTTTG TCTTTGGGCC CACAGGCTGC AACCTTGAGG GCTTCTTTGC CACCCTTGGA 
GGTGAAATCG GCCTGTGGTC CCTGGTAGTC CTGGCCATTG AGCGCTACGT GGTGGTCTGC 
AAGCCCATGA GCAACTTCCG CTTTGGGGAG AATCATGCCA TTATGGGTGT GGCCTTCACC 
TGGGTCATGG CGTTGGCCTG TGCTGCTCCC CCACTGGTTG GCTGGTCCAG GTACATCCCC 
GAGGGCATGC AGTGTTCATG TGGGATTGAC TACTATACAC TCAAGCCTGA GGTCAACAAT 
GAGTCCTTCG TCATCTACAT GTTCGTGGTC CACTTCACCA TCCCCATGAT CGTCATCTTC 
TTCTGCTACG GGCAGCTGGT CTTCACCGTC AAGGAGGCCG CCGCCCAGCA ACAGGAGTCG 
GCTACCACTC AGAAGGCAGA GAAGGAAGTC ACGCGCATGG TCATCATCAT GGTCATCTTC 
TTCCTGATCT GCTGGCTTCC CTATGCCAGT GTGGCCATGT ACATCTTTAC CCACCAGGGC 
TCCAACTTCG GCCCCATCTT CATGACCCTT CCCGCTTTCT TTGCTAAGAC CGCCTCCATC 
TACAACCCAA TCATCTACAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GCTCACCACG 
CTCTGCTGCG GCAAGAATCC ACTGGGAGAT GATGAGGCCT CTGCCACTGC CTCCAAGACG 
GAGACCAGCC AGGTGGCTCC AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.
The American journal of pathology187(10)2222-2231(2017 Oct)
Malechka VV,Moiseyev G,Takahashi Y,Shin Y,Ma JX

Allele-Specific Inhibition of Rhodopsin With an Antisense Oligonucleotide Slows Photoreceptor Cell Degeneration.
Investigative ophthalmology & visual science56(11)6362-75(2015 Oct)
Murray SF,Jazayeri A,Matthes MT,Yasumura D,Yang H,Peralta R,Watt A,Freier S,Hung G,Adamson PS,Guo S,Monia BP,LaVail MM,McCaleb ML

Epidermal growth factor induces Ca(2+) sensitization through Rho-kinase-dependent phosphorylation of myosin phosphatase target subunit 1 in vascular smooth muscle.
European journal of pharmacology76289-95(2015 Sep)
Sasahara T,Okamoto H,Ohkura N,Kobe A,Yayama K

Ultrastructural visualization of trans-ciliary rhodopsin cargoes in mammalian rods.
Cilia44(2015)
Chuang JZ,Hsu YC,Sung CH

Photoreceptor degeneration, structural remodeling and glial activation: a morphological study on a genetic mouse model for pericyte deficiency.
Neuroscience279269-84(2014 Oct)
Genov? G,Mollick T,Johansson K

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Rho cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報