RHO cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHO
Entrez Gene ID	6010
Full Name	rhodopsin
Synonyms	CSNBAD1,OPN2,RP4
General protein information	Preferred Names rhodopsin Names rhodopsin opsin 2, rod pigment opsin-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q22.1
Summary	The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness. [provided by RefSeq, Aug 2017].
Disorder MIM:	180380
Disorder Html:	Retinitis pigmentosa 4, autosomal dominant or recessive, 613731 (3); Night blindness, congenital stationary, autosomal dominant 1, 610445 (3); Retinitis punctata albescens, 136880 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000539.3	NP_000530.1	rhodopsin

Pathways from BioSystems

Homologies

	RHO	XP_001094250.1
Canis lupus familiaris (dog)	RHO	NP_001008277.1
Mus musculus (house mouse)	Rho	NP_663358.1
Xenopus tropicalis (tropical clawed frog)	rho	NP_001090803.1
Homo sapiens (human)	RHO	NP_000530.1
Pan troglodytes (chimpanzee)	RHO	XP_516740.2
Gallus gallus (chicken)	RHO	NP_001025777.1
Bos taurus (cattle)	RHO	NP_001014890.1
Rattus norvegicus (Norway rat)	Rho	NP_254276.1
Danio rerio (zebrafish)	exorh	NP_571287.1

Gene Ontology
Bibliography

Related articles in PubMed

oxLDL induces endothelial cell proliferation via Rho/ROCK/Akt/p27
Zhang C, Adamos C, Oh MJ, Baruah J, Ayee MAA, Mehta D, Wary KK, Levitan I
American journal of physiology. Cell physiology313(3)C340-C351(2017 Sep)

Identification of Phosphorylation Codes for Arrestin Recruitment by G Protein-Coupled Receptors.
Zhou XE, He Y, de Waal PW, Gao X, Kang Y, Van Eps N, Yin Y, Pal K, Goswami D, White TA, Barty A, Latorraca NR, Chapman HN, Hubbell WL, Dror RO, Stevens RC, Cherezov V, Gurevich VV, Griffin PR, Ernst OP, Melcher K, Xu HE
Cell170(3)457-469.e13(2017 Jul)

Effects of Pathogenic Variations in the Human Rhodopsin Gene (hRHO) on the Predicted Accessibility for a Lead Candidate Ribozyme.
Froebel BR, Trujillo AJ, Sullivan JM
Investigative ophthalmology & visual science58(9)3576-3591(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data provide the first evidence that T17M rhodopsin mutant disrupts C3 secretion via the induction of ROS and the suppression of TWIST1.
Title: Rhodopsin T17M Mutant Inhibits Complement C3 Secretion in Retinal Pigment Epithelium via ROS Induced Downregulation of TWIST1.

These findings provide a new understanding of the effects of oxLDL on endothelial proliferation, which is essential for developing new treatments against neovascularization and progression of atherosclerosis.
Title: oxLDL induces endothelial cell proliferation via Rho/ROCK/Akt/p27

Study reports an X-ray free electron laser crystal structure of the rhodopsin-arrestin complex, in which the phosphorylated C terminus of rhodopsin forms an extended intermolecular beta sheet with the N-terminal beta strands of arrestin. Phosphorylation was detected at rhodopsin C-terminal tail residues T336 and S338.
Title: Identification of Phosphorylation Codes for Arrestin Recruitment by G Protein-Coupled Receptors.

A mutation-independent strategy appears viable in this specific context but certain mutations could significantly influence ribozyme or RNAi efficacy through impact on accessibility at the target annealing site/region.
Title: Effects of Pathogenic Variations in the Human Rhodopsin Gene (hRHO) on the Predicted Accessibility for a Lead Candidate Ribozyme.

Data suggest that retinitis pigmentosa-associated mutation G51A behaves differently in human rhodopsin compared to bovine rhodopsin; human rhodopsin is more thermally stable than ancestral ancestrally reconstructed mammalian rhodopsin.
Title: An experimental comparison of human and bovine rhodopsin provides insight into the molecular basis of retinal disease.

The following RHO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu27011	NM_000539.3 Latest version!	Homo sapiens rhodopsin (RHO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHO ( NM_000539.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000530.1 Homo sapiens rhodopsin (RHO), mRNA.

Price & Availability↑

CloneID	OHu27011
Clone ID Related Accession (Same CDS sequence)	NM_000539.3
Accession Version	NM_000539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	rhodopsin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC080007.26. This sequence is a reference standard in the RefSeqGene project. On Mar 13, 2008 this sequence version replaced NM_000539.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537381.1, BC112104.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2142680, SAMEA2144333 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000296271.4/ ENSP00000296271.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAATGGCA CAGAAGGCCC TAACTTCTAC GTGCCCTTCT CCAATGCGAC GGGTGTGGTA 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCCGCCTACA TGTTTCTGCT GATCGTGCTG GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACCGTCC AGCACAAGAA GCTGCGCACG CCTCTCAACT ACATCCTGCT CAACCTAGCC 
GTGGCTGACC TCTTCATGGT CCTAGGTGGC TTCACCAGCA CCCTCTACAC CTCTCTGCAT 
GGATACTTCG TCTTCGGGCC CACAGGATGC AATTTGGAGG GCTTCTTTGC CACCCTGGGC 
GGTGAAATTG CCCTGTGGTC CTTGGTGGTC CTGGCCATCG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG CTTCGGGGAG AACCATGCCA TCATGGGCGT TGCCTTCACC 
TGGGTCATGG CGCTGGCCTG CGCCGCACCC CCACTCGCCG GCTGGTCCAG GTACATCCCC 
GAGGGCCTGC AGTGCTCGTG TGGAATCGAC TACTACACGC TCAAGCCGGA GGTCAACAAC 
GAGTCTTTTG TCATCTACAT GTTCGTGGTC CACTTCACCA TCCCCATGAT TATCATCTTT 
TTCTGCTATG GGCAGCTCGT CTTCACCGTC AAGGAGGCCG CTGCCCAGCA GCAGGAGTCA 
GCCACCACAC AGAAGGCAGA GAAGGAGGTC ACCCGCATGG TCATCATCAT GGTCATCGCT 
TTCCTGATCT GCTGGGTGCC CTACGCCAGC GTGGCATTCT ACATCTTCAC CCACCAGGGC 
TCCAACTTCG GTCCCATCTT CATGACCATC CCAGCGTTCT TTGCCAAGAG CGCCGCCATC 
TACAACCCTG TCATCTATAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GCTCACCACC 
ATCTGCTGCG GCAAGAACCC ACTGGGTGAC GATGAGGCCT CTGCTACCGT GTCCAAGACG 
GAGACGAGCC AGGTGGCCCC GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000530.1
CDS	96..1142
Translation	MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA

Target ORF information:

RefSeq Version	NM_000539.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rhodopsin (RHO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000539.3

ORF Insert Sequence:

ATGAATGGCA CAGAAGGCCC TAACTTCTAC GTGCCCTTCT CCAATGCGAC GGGTGTGGTA 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCCGCCTACA TGTTTCTGCT GATCGTGCTG GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACCGTCC AGCACAAGAA GCTGCGCACG CCTCTCAACT ACATCCTGCT CAACCTAGCC 
GTGGCTGACC TCTTCATGGT CCTAGGTGGC TTCACCAGCA CCCTCTACAC CTCTCTGCAT 
GGATACTTCG TCTTCGGGCC CACAGGATGC AATTTGGAGG GCTTCTTTGC CACCCTGGGC 
GGTGAAATTG CCCTGTGGTC CTTGGTGGTC CTGGCCATCG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG CTTCGGGGAG AACCATGCCA TCATGGGCGT TGCCTTCACC 
TGGGTCATGG CGCTGGCCTG CGCCGCACCC CCACTCGCCG GCTGGTCCAG GTACATCCCC 
GAGGGCCTGC AGTGCTCGTG TGGAATCGAC TACTACACGC TCAAGCCGGA GGTCAACAAC 
GAGTCTTTTG TCATCTACAT GTTCGTGGTC CACTTCACCA TCCCCATGAT TATCATCTTT 
TTCTGCTATG GGCAGCTCGT CTTCACCGTC AAGGAGGCCG CTGCCCAGCA GCAGGAGTCA 
GCCACCACAC AGAAGGCAGA GAAGGAGGTC ACCCGCATGG TCATCATCAT GGTCATCGCT 
TTCCTGATCT GCTGGGTGCC CTACGCCAGC GTGGCATTCT ACATCTTCAC CCACCAGGGC 
TCCAACTTCG GTCCCATCTT CATGACCATC CCAGCGTTCT TTGCCAAGAG CGCCGCCATC 
TACAACCCTG TCATCTATAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GCTCACCACC 
ATCTGCTGCG GCAAGAACCC ACTGGGTGAC GATGAGGCCT CTGCTACCGT GTCCAAGACG 
GAGACGAGCC AGGTGGCCCC GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

oxLDL induces endothelial cell proliferation via Rho/ROCK/Akt/p27
American journal of physiology. Cell physiology313(3)C340-C351(2017 Sep)
Zhang C,Adamos C,Oh MJ,Baruah J,Ayee MAA,Mehta D,Wary KK,Levitan I

Identification of Phosphorylation Codes for Arrestin Recruitment by G Protein-Coupled Receptors.
Cell170(3)457-469.e13(2017 Jul)
Zhou XE,He Y,de Waal PW,Gao X,Kang Y,Van Eps N,Yin Y,Pal K,Goswami D,White TA,Barty A,Latorraca NR,Chapman HN,Hubbell WL,Dror RO,Stevens RC,Cherezov V,Gurevich VV,Griffin PR,Ernst OP,Melcher K,Xu HE

Effects of Pathogenic Variations in the Human Rhodopsin Gene (hRHO) on the Predicted Accessibility for a Lead Candidate Ribozyme.
Investigative ophthalmology & visual science58(9)3576-3591(2017 Jul)
Froebel BR,Trujillo AJ,Sullivan JM

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RHO cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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