RHO cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHO
Entrez Gene ID	493763
Full Name	rhodopsin
Synonyms	RHO1
General protein information	Preferred Names rhodopsin Names rhodopsin opsin rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) rod opsin
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005632035.2	XP_005632092.1	rhodopsin isoform X1
XM_005632035.3	XP_005632092.1	rhodopsin isoform X2
XM_022406252.1	XP_022261960.1	rhodopsin isoform X1
NM_001008276.1	NP_001008277.1	rhodopsin

Pathways from BioSystems

Homologies

	RHO	XP_001094250.1
Canis lupus familiaris (dog)	RHO	NP_001008277.1
Mus musculus (house mouse)	Rho	NP_663358.1
Xenopus tropicalis (tropical clawed frog)	rho	NP_001090803.1
Homo sapiens (human)	RHO	NP_000530.1
Pan troglodytes (chimpanzee)	RHO	XP_516740.2
Gallus gallus (chicken)	RHO	NP_001025777.1
Bos taurus (cattle)	RHO	NP_001014890.1
Rattus norvegicus (Norway rat)	Rho	NP_254276.1
Danio rerio (zebrafish)	exorh	NP_571287.1

Gene Ontology
Bibliography

Related articles in PubMed

In vivo dynamics of retinal injury and repair in the rhodopsin mutant dog model of human retinitis pigmentosa.
Cideciyan AV, Jacobson SG, Aleman TS, Gu D, Pearce-Kelling SE, Sumaroka A, Acland GM, Aguirre GD
Proceedings of the National Academy of Sciences of the United States of America102(14)5233-8(2005 Apr)

Naturally occurring rhodopsin mutation in the dog causes retinal dysfunction and degeneration mimicking human dominant retinitis pigmentosa.
Kijas JW, Cideciyan AV, Aleman TS, Pianta MJ, Pearce-Kelling SE, Miller BJ, Jacobson SG, Aguirre GD, Acland GM
Proceedings of the National Academy of Sciences of the United States of America99(9)6328-33(2002 Apr)

The introns of the canine rod opsin gene show higher sequence homology to the human than to the rodent introns.
Kylm? T, Roos C, Paulin L, Kommonen B
DNA sequence : the journal of DNA sequencing and mapping8(1-2)99-104(1997)

Nucleotide sequence of the canine rod-opsin-encoding gene.
Petersen-Jones SM, Sohal AK, Sargan DR
Gene143(2)281-4(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mechanisms of retinal neuronal injury, repair, compensation, and degeneration in the rhodopsin mutant dog
Title: In vivo dynamics of retinal injury and repair in the rhodopsin mutant dog model of human retinitis pigmentosa.

The following RHO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe34296	NM_001008276.1 Latest version!	Canis lupus familiaris rhodopsin (RHO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe21123	XM_005632035.3 Latest version!	Canis lupus familiaris rhodopsin (RHO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe295203	XM_022406252.1 Latest version!	Canis lupus familiaris rhodopsin (RHO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OCe21123	XM_005632035.2	Canis lupus familiaris rhodopsin (RHO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHO ( NM_001008276.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001008277.1 Canis lupus familiaris rhodopsin (RHO), mRNA.

Price & Availability↑

CloneID	OCe34296
Clone ID Related Accession (Same CDS sequence)	NM_001008276.1
Accession Version	NM_001008276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Canis lupus familiaris(dog)
Product	rhodopsin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y09004.1. On Feb 9, 2005 this sequence version replaced XM_533733.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA2240903 [ECO:0000350] ##Evidence-Data-END##

ATGAACGGGA CGGAGGGCCC GAACTTCTAC GTGCCCTTCT CCAACAAGAC GGGTGTGGTG 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCTGCCTACA TGTTTCTGCT GATCGTGCTC GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACAGTCC AGCACAAGAA GCTGCGTACA CCTCTCAACT ACATCCTGCT CAACCTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGTGGC TTCACCACCA CCCTCTACAC CTCTCTGCAT 
GGATACTTTG TCTTCGGGCC CACAGGATGC AATGTGGAGG GCTTCTTTGC CACACTGGGC 
GGTGAAATTG CCCTGTGGTC TTTGGTGGTC CTGGCCATTG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG TTTTGGGGAG AACCATGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CACTGGCCTG TGCAGCACCC CCCCTCGCTG GCTGGTCCAG TCTCCTGTCT 
CACTCCCCTC TGGTGCTCAG GTACATCCCA GAGGGCATGC AGTGCTCATG TGGGATCGAC 
TACTACACAC TCAAGCCAGA AATCAACAAT GAGTCCTTCG TCATCTACAT GTTCGTGGTC 
CACTTCGCCA TCCCCATGAT TGTCATATTC TTCTGCTATG GACAGCTCGT CTTCACAGTC 
AAGGAGGCAG CTGCCCAGCA GCAGGAATCG GCCACCACCC AGAAGGCTGA AAAGGAGGTC 
ACCCGCATGG TCATCATCAT GGTCATCGCT TTCCTGATCT GCTGGGTGCC CTATGCCAGT 
GTGGCATTCT ACATCTTCAC CCACCAGGGC TCCGACTTTG GCCCCATCTT CATGACCCTC 
CCAGCGTTCT TCGCCAAGTC CTCCTCCATC TACAACCCTG TCATCTATAT CATGATGAAC 
AAGCAGTTCC GGAACTGCAT GATCACCACC CTCTGCTGTG GCAAGAACCC ACTGGGTGAC 
GACGAGGCCT CTGCCAGCGC CTCCAAGACG GAGACCAGCC AGGTGGCACC GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008277.1
CDS	1..1077
Translation	MNGTEGPNFYVPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNVEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSSLLSHSPLVLRYIPEGMQCSCGIDYYTLKPEINNESFVIYMFVVHFAIPMIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSDFGPIFMTLPAFFAKSSSIYNPVIYIMMNKQFRNCMITTLCCGKNPLGDDEASASASKTETSQVAPA

Target ORF information:

RefSeq Version	NM_001008276.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhodopsin (RHO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008276.1

ORF Insert Sequence:

ATGAACGGGA CGGAGGGCCC GAACTTCTAC GTGCCCTTCT CCAACAAGAC GGGTGTGGTG 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCTGCCTACA TGTTTCTGCT GATCGTGCTC GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACAGTCC AGCACAAGAA GCTGCGTACA CCTCTCAACT ACATCCTGCT CAACCTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGTGGC TTCACCACCA CCCTCTACAC CTCTCTGCAT 
GGATACTTTG TCTTCGGGCC CACAGGATGC AATGTGGAGG GCTTCTTTGC CACACTGGGC 
GGTGAAATTG CCCTGTGGTC TTTGGTGGTC CTGGCCATTG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG TTTTGGGGAG AACCATGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CACTGGCCTG TGCAGCACCC CCCCTCGCTG GCTGGTCCAG TCTCCTGTCT 
CACTCCCCTC TGGTGCTCAG GTACATCCCA GAGGGCATGC AGTGCTCATG TGGGATCGAC 
TACTACACAC TCAAGCCAGA AATCAACAAT GAGTCCTTCG TCATCTACAT GTTCGTGGTC 
CACTTCGCCA TCCCCATGAT TGTCATATTC TTCTGCTATG GACAGCTCGT CTTCACAGTC 
AAGGAGGCAG CTGCCCAGCA GCAGGAATCG GCCACCACCC AGAAGGCTGA AAAGGAGGTC 
ACCCGCATGG TCATCATCAT GGTCATCGCT TTCCTGATCT GCTGGGTGCC CTATGCCAGT 
GTGGCATTCT ACATCTTCAC CCACCAGGGC TCCGACTTTG GCCCCATCTT CATGACCCTC 
CCAGCGTTCT TCGCCAAGTC CTCCTCCATC TACAACCCTG TCATCTATAT CATGATGAAC 
AAGCAGTTCC GGAACTGCAT GATCACCACC CTCTGCTGTG GCAAGAACCC ACTGGGTGAC 
GACGAGGCCT CTGCCAGCGC CTCCAAGACG GAGACCAGCC AGGTGGCACC GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHO ( XM_005632035.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632092.1 Canis lupus familiaris rhodopsin (RHO), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe21123
Clone ID Related Accession (Same CDS sequence)	XM_005632035.2 , XM_005632035.3
Accession Version	XM_005632035.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rhodopsin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726095.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005632035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGGA CGGAGGGCCC GAACTTCTAC GTGCCCTTCT CCAACAAGAC GGGTGTGGTG 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCTGCCTACA TGTTTCTGCT GATCGTGCTC GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACAGTCC AGCACAAGAA GCTGCGTACA CCTCTCAACT ACATCCTGCT CAACCTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGTGGC TTCACCACCA CCCTCTACAC CTCTCTGCAT 
GGATACTTTG TCTTCGGGCC CACAGGATGC AATGTGGAGG GCTTCTTTGC CACACTGGGC 
GGTGAAATTG CCCTGTGGTC TTTGGTGGTC CTGGCCATTG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG TTTTGGGGAG AACCATGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CGCTGGCCTG TGCGGCACCC CCCCTCGCTG GCTGGTCCAG GTACATCCCA 
GAGGGCATGC AGTGCTCATG TGGGATCGAC TACTACACAC TCAAGCCAGA AATCAACAAT 
GAGTCCTTCG TCATCTACAT GTTCGTGGTC CACTTCGCCA TCCCCATGAT TGTCATATTC 
TTCTGCTATG GACAGCTCGT CTTCACAGTC AAGGAGGCAG CTGCCCAGCA GCAGGAATCG 
GCCACCACCC AGAAGGCTGA AAAGGAGGTC ACCCGCATGG TCATCATCAT GGTCATCGCT 
TTCCTGATCT GCTGGGTGCC CTATGCCAGT GTGGCATTCT ACATCTTCAC CCACCAGGGC 
TCCGACTTTG GCCCCATCTT CATGACCCTC CCAGCGTTCT TCGCCAAGTC CTCCTCCATC 
TACAACCCTG TCATCTATAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GATCACCACC 
CTCTGCTGTG GCAAGAACCC ACTGGGTGAC GACGAGGCCT CTGCCAGCGC CTCCAAGACG 
GAGACCAGCC AGGTGGCACC GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632092.1
CDS	1..1047
Translation	MNGTEGPNFYVPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNVEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGMQCSCGIDYYTLKPEINNESFVIYMFVVHFAIPMIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSDFGPIFMTLPAFFAKSSSIYNPVIYIMMNKQFRNCMITTLCCGKNPLGDDEASASASKTETSQVAPA

Target ORF information:

RefSeq Version	XM_005632035.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rhodopsin (RHO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632035.2

ORF Insert Sequence:

ATGAACGGGA CGGAGGGCCC GAACTTCTAC GTGCCCTTCT CCAACAAGAC GGGTGTGGTG 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCTGCCTACA TGTTTCTGCT GATCGTGCTC GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACAGTCC AGCACAAGAA GCTGCGTACA CCTCTCAACT ACATCCTGCT CAACCTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGTGGC TTCACCACCA CCCTCTACAC CTCTCTGCAT 
GGATACTTTG TCTTCGGGCC CACAGGATGC AATGTGGAGG GCTTCTTTGC CACACTGGGC 
GGTGAAATTG CCCTGTGGTC TTTGGTGGTC CTGGCCATTG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG TTTTGGGGAG AACCATGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CGCTGGCCTG TGCGGCACCC CCCCTCGCTG GCTGGTCCAG GTACATCCCA 
GAGGGCATGC AGTGCTCATG TGGGATCGAC TACTACACAC TCAAGCCAGA AATCAACAAT 
GAGTCCTTCG TCATCTACAT GTTCGTGGTC CACTTCGCCA TCCCCATGAT TGTCATATTC 
TTCTGCTATG GACAGCTCGT CTTCACAGTC AAGGAGGCAG CTGCCCAGCA GCAGGAATCG 
GCCACCACCC AGAAGGCTGA AAAGGAGGTC ACCCGCATGG TCATCATCAT GGTCATCGCT 
TTCCTGATCT GCTGGGTGCC CTATGCCAGT GTGGCATTCT ACATCTTCAC CCACCAGGGC 
TCCGACTTTG GCCCCATCTT CATGACCCTC CCAGCGTTCT TCGCCAAGTC CTCCTCCATC 
TACAACCCTG TCATCTATAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GATCACCACC 
CTCTGCTGTG GCAAGAACCC ACTGGGTGAC GACGAGGCCT CTGCCAGCGC CTCCAAGACG 
GAGACCAGCC AGGTGGCACC GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHO ( XM_005632035.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632092.1 Canis lupus familiaris rhodopsin (RHO), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe21123
Clone ID Related Accession (Same CDS sequence)	XM_005632035.2 , XM_005632035.3
Accession Version	XM_005632035.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rhodopsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005632035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGGA CGGAGGGCCC GAACTTCTAC GTGCCCTTCT CCAACAAGAC GGGTGTGGTG 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCTGCCTACA TGTTTCTGCT GATCGTGCTC GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACAGTCC AGCACAAGAA GCTGCGTACA CCTCTCAACT ACATCCTGCT CAACCTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGTGGC TTCACCACCA CCCTCTACAC CTCTCTGCAT 
GGATACTTTG TCTTCGGGCC CACAGGATGC AATGTGGAGG GCTTCTTTGC CACACTGGGC 
GGTGAAATTG CCCTGTGGTC TTTGGTGGTC CTGGCCATTG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG TTTTGGGGAG AACCATGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CGCTGGCCTG TGCGGCACCC CCCCTCGCTG GCTGGTCCAG GTACATCCCA 
GAGGGCATGC AGTGCTCATG TGGGATCGAC TACTACACAC TCAAGCCAGA AATCAACAAT 
GAGTCCTTCG TCATCTACAT GTTCGTGGTC CACTTCGCCA TCCCCATGAT TGTCATATTC 
TTCTGCTATG GACAGCTCGT CTTCACAGTC AAGGAGGCAG CTGCCCAGCA GCAGGAATCG 
GCCACCACCC AGAAGGCTGA AAAGGAGGTC ACCCGCATGG TCATCATCAT GGTCATCGCT 
TTCCTGATCT GCTGGGTGCC CTATGCCAGT GTGGCATTCT ACATCTTCAC CCACCAGGGC 
TCCGACTTTG GCCCCATCTT CATGACCCTC CCAGCGTTCT TCGCCAAGTC CTCCTCCATC 
TACAACCCTG TCATCTATAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GATCACCACC 
CTCTGCTGTG GCAAGAACCC ACTGGGTGAC GACGAGGCCT CTGCCAGCGC CTCCAAGACG 
GAGACCAGCC AGGTGGCACC GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632092.1
CDS	1503..2549
Translation	MNGTEGPNFYVPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNVEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGMQCSCGIDYYTLKPEINNESFVIYMFVVHFAIPMIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSDFGPIFMTLPAFFAKSSSIYNPVIYIMMNKQFRNCMITTLCCGKNPLGDDEASASASKTETSQVAPA

Target ORF information:

RefSeq Version	XM_005632035.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhodopsin (RHO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632035.3

ORF Insert Sequence:

ATGAACGGGA CGGAGGGCCC GAACTTCTAC GTGCCCTTCT CCAACAAGAC GGGTGTGGTG 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCTGCCTACA TGTTTCTGCT GATCGTGCTC GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACAGTCC AGCACAAGAA GCTGCGTACA CCTCTCAACT ACATCCTGCT CAACCTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGTGGC TTCACCACCA CCCTCTACAC CTCTCTGCAT 
GGATACTTTG TCTTCGGGCC CACAGGATGC AATGTGGAGG GCTTCTTTGC CACACTGGGC 
GGTGAAATTG CCCTGTGGTC TTTGGTGGTC CTGGCCATTG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG TTTTGGGGAG AACCATGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CGCTGGCCTG TGCGGCACCC CCCCTCGCTG GCTGGTCCAG GTACATCCCA 
GAGGGCATGC AGTGCTCATG TGGGATCGAC TACTACACAC TCAAGCCAGA AATCAACAAT 
GAGTCCTTCG TCATCTACAT GTTCGTGGTC CACTTCGCCA TCCCCATGAT TGTCATATTC 
TTCTGCTATG GACAGCTCGT CTTCACAGTC AAGGAGGCAG CTGCCCAGCA GCAGGAATCG 
GCCACCACCC AGAAGGCTGA AAAGGAGGTC ACCCGCATGG TCATCATCAT GGTCATCGCT 
TTCCTGATCT GCTGGGTGCC CTATGCCAGT GTGGCATTCT ACATCTTCAC CCACCAGGGC 
TCCGACTTTG GCCCCATCTT CATGACCCTC CCAGCGTTCT TCGCCAAGTC CTCCTCCATC 
TACAACCCTG TCATCTATAT CATGATGAAC AAGCAGTTCC GGAACTGCAT GATCACCACC 
CTCTGCTGTG GCAAGAACCC ACTGGGTGAC GACGAGGCCT CTGCCAGCGC CTCCAAGACG 
GAGACCAGCC AGGTGGCACC GGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHO ( XM_022406252.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022261960.1 Canis lupus familiaris rhodopsin (RHO), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe295203
Clone ID Related Accession (Same CDS sequence)	XM_022406252.1
Accession Version	XM_022406252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rhodopsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGGA CGGAGGGCCC GAACTTCTAC GTGCCCTTCT CCAACAAGAC GGGTGTGGTG 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCTGCCTACA TGTTTCTGCT GATCGTGCTC GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACAGTCC AGCACAAGAA GCTGCGTACA CCTCTCAACT ACATCCTGCT CAACCTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGTGGC TTCACCACCA CCCTCTACAC CTCTCTGCAT 
GGATACTTTG TCTTCGGGCC CACAGGATGC AATGTGGAGG GCTTCTTTGC CACACTGGGC 
GGTGAAATTG CCCTGTGGTC TTTGGTGGTC CTGGCCATTG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG TTTTGGGGAG AACCATGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CGCTGGCCTG TGCGGCACCC CCCCTCGCTG GCTGGTCCAG GTACATCCCA 
GAGGGCATGC AGTGCTCATG TGGGATCGAC TACTACACAC TCAAGCCAGA AATCAACAAT 
GAGTCCTTCG TCATCTACAT GTTCGTGGTC CACTTCGCCA TCCCCATGAT TGTCATATTC 
TTCTGCTATG GACAGCTCGT CTTCACAGTC AAGGAGGCAG CTGCCCAGCA GCAGGAATCG 
GCCACCACCC AGAAGGCTGA AAAGGAGGTC ACCCGCATGG TCATCATCAT GGTCATCGCT 
TTCCTGATCT GCTGGGTGCC CTATGCCAGT GTGGCATTCT ACATCTTCAC CCACCAGGGC 
TCCGACTTTG GCCCCATCTT CATGACCCTC CCAGCGTTCT TCGCCAAGTC CTCCTCCATC 
TACAACCCTG TCATCTATAT CATGATGAAC AAGCAGGTGC CTGCTGGCGG GACAGGCGGG 
TCCAGGGGCC CCAGGCTTCA GGCACTGCCT GTGGAGGACA AGCCGTGTCC TCAGTCATTC 
CACTGGCAAA ACCAGGCAGC GAAACTGGCA TCCCCCAGGG CATCGGTCAT ATGGGAGAAA 
TGCAGATTCC TGCTGAATCA GAAGCTCGCG GTAGGCCCAG GAATTTGCAT TTCTAACAAG 
CCCTCCAGGT GTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022261960.1
CDS	1503..2660
Translation	MNGTEGPNFYVPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNVEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGMQCSCGIDYYTLKPEINNESFVIYMFVVHFAIPMIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSDFGPIFMTLPAFFAKSSSIYNPVIYIMMNKQVPAGGTGGSRGPRLQALPVEDKPCPQSFHWQNQAAKLASPRASVIWEKCRFLLNQKLAVGPGICISNKPSRCL

Target ORF information:

RefSeq Version	XM_022406252.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhodopsin (RHO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406252.1

ORF Insert Sequence:

ATGAACGGGA CGGAGGGCCC GAACTTCTAC GTGCCCTTCT CCAACAAGAC GGGTGTGGTG 
CGCAGCCCCT TCGAGTACCC ACAGTACTAC CTGGCTGAGC CATGGCAGTT CTCCATGCTG 
GCTGCCTACA TGTTTCTGCT GATCGTGCTC GGCTTCCCCA TCAACTTCCT CACGCTCTAC 
GTCACAGTCC AGCACAAGAA GCTGCGTACA CCTCTCAACT ACATCCTGCT CAACCTGGCT 
GTGGCTGACC TCTTCATGGT CTTCGGTGGC TTCACCACCA CCCTCTACAC CTCTCTGCAT 
GGATACTTTG TCTTCGGGCC CACAGGATGC AATGTGGAGG GCTTCTTTGC CACACTGGGC 
GGTGAAATTG CCCTGTGGTC TTTGGTGGTC CTGGCCATTG AGCGGTACGT GGTGGTGTGT 
AAGCCCATGA GCAACTTCCG TTTTGGGGAG AACCATGCCA TCATGGGCGT CGCCTTCACC 
TGGGTCATGG CGCTGGCCTG TGCGGCACCC CCCCTCGCTG GCTGGTCCAG GTACATCCCA 
GAGGGCATGC AGTGCTCATG TGGGATCGAC TACTACACAC TCAAGCCAGA AATCAACAAT 
GAGTCCTTCG TCATCTACAT GTTCGTGGTC CACTTCGCCA TCCCCATGAT TGTCATATTC 
TTCTGCTATG GACAGCTCGT CTTCACAGTC AAGGAGGCAG CTGCCCAGCA GCAGGAATCG 
GCCACCACCC AGAAGGCTGA AAAGGAGGTC ACCCGCATGG TCATCATCAT GGTCATCGCT 
TTCCTGATCT GCTGGGTGCC CTATGCCAGT GTGGCATTCT ACATCTTCAC CCACCAGGGC 
TCCGACTTTG GCCCCATCTT CATGACCCTC CCAGCGTTCT TCGCCAAGTC CTCCTCCATC 
TACAACCCTG TCATCTATAT CATGATGAAC AAGCAGGTGC CTGCTGGCGG GACAGGCGGG 
TCCAGGGGCC CCAGGCTTCA GGCACTGCCT GTGGAGGACA AGCCGTGTCC TCAGTCATTC 
CACTGGCAAA ACCAGGCAGC GAAACTGGCA TCCCCCAGGG CATCGGTCAT ATGGGAGAAA 
TGCAGATTCC TGCTGAATCA GAAGCTCGCG GTAGGCCCAG GAATTTGCAT TTCTAACAAG 
CCCTCCAGGT GTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In vivo dynamics of retinal injury and repair in the rhodopsin mutant dog model of human retinitis pigmentosa.
Proceedings of the National Academy of Sciences of the United States of America102(14)5233-8(2005 Apr)
Cideciyan AV,Jacobson SG,Aleman TS,Gu D,Pearce-Kelling SE,Sumaroka A,Acland GM,Aguirre GD

Naturally occurring rhodopsin mutation in the dog causes retinal dysfunction and degeneration mimicking human dominant retinitis pigmentosa.
Proceedings of the National Academy of Sciences of the United States of America99(9)6328-33(2002 Apr)
Kijas JW,Cideciyan AV,Aleman TS,Pianta MJ,Pearce-Kelling SE,Miller BJ,Jacobson SG,Aguirre GD,Acland GM

The introns of the canine rod opsin gene show higher sequence homology to the human than to the rodent introns.
DNA sequence : the journal of DNA sequencing and mapping8(1-2)99-104(1997)
Kylm? T,Roos C,Paulin L,Kommonen B

Nucleotide sequence of the canine rod-opsin-encoding gene.
Gene143(2)281-4(1994 Jun)
Petersen-Jones SM,Sohal AK,Sargan DR

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RHO cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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