DIO3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIO3
Entrez Gene ID	494549
Full Name	iodothyronine deiodinase 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. It is highly expressed in placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It thus plays a critical role in mammalian development by regulating circulating fetal thyroid hormone concentration. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001010993.3	NP_001010993.2	thyroxine 5-deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO3	NP_001353.4
Rattus norvegicus (Norway rat)	Dio3	NP_058906.3
Gallus gallus (chicken)	DIO3	NP_001116120.1
Xenopus tropicalis (tropical clawed frog)	dio3	NP_001107139.2
	DIO3	NP_001116121.1
Canis lupus familiaris (dog)	DIO3	NP_001157660.1
Danio rerio (zebrafish)	dio3a	NP_001242932.1
Bos taurus (cattle)	DIO3	NP_001010993.2
Mus musculus (house mouse)	Dio3	NP_742117.2
Danio rerio (zebrafish)	dio3b	NP_001171406.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression and imprinting of DIO3 and DIO3OS genes in Holstein cattle.
Yang W, Li D, Wang G, Wu X, Zhang M, Zhang C, Cui Y, Li S
Journal of genetics96(2)333-339(2017 Jun)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Molecular cloning, expression and radiation hybrid mapping of the bovine deiodinase type II (DIO2) and deiodinase type III (DIO3) genes.
Connor EE, Laiakis EC, Fernandes VM, Williams JL, Capuco AV
Animal genetics36(3)240-3(2005 Jun)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DIO3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIO3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01500	NM_001010993.3 Latest version!	Bos taurus iodothyronine deiodinase 3 (DIO3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIO3 ( NM_001010993.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001010993.2 Bos taurus iodothyronine deiodinase 3 (DIO3), mRNA.

Price & Availability↑

CloneID	OBa01500
Clone ID Related Accession (Same CDS sequence)	NM_001010993.3
Accession Version	NM_001010993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Bos taurus(cattle)
Product	thyroxine 5-deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from NKLS02000021.1, AW481205.1 and AY858552.1. On Mar 7, 2012 this sequence version replaced NM_001010993.2. ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGCC AGGCTGCCCC GCGGTGGGTG GTGGGGGAAG GTAGGGGGAC TTTGGGGGGC 
GCCGCCACCA TGCTGCGCTC CCTGCTGCTT CACTCCCTGA GGCTCTGCTC CCAGACGGCC 
TCGTGCCTCG TGCTCTTCCC GCGCTTCCTG GGCACGGCCT TCATGCTCTG GCTGCTAGAC 
TTCCTGTGCA TCCGTAAGCA TTTACTGGGC CGTCGACGCC GGGGTCAGCC GGAAATCGAA 
GTGGAGCTCA ACAGCGATGG CGAGGAGGTG CCCCCCGACG ACCCGCCTGT CTGCGTGTCC 
GACGACAACC GCCTGTGCAC CTTGGCGTCG CTGAGGGCCG TCTGGCACGG CCAGAAGTTG 
GATTTCTTCA AGCAGGCGCA TGAAGGCGGG CCGGCGCCCA ACTCTGAGGT GGTCCTGCCC 
GACGGCTTCC AGAACCAGCA CATCCTCGAC TACGCCCGAG GAAACCGTCC GCTGGTGCTC 
AACTTCGGCA GCTGCACCTG ACCACCGTTC ATGGCGCGCA TGAGCGCCTT CCAGCGCCTG 
GTCACCAAGT ATCAGCGCGA CGTCGACTTC CTCATCATCT ACATCGAGGA AGCTCACCCT 
TCCGACGGCT GGGTCACCAC AGACTCTCCC TACAGCATCC CGCAGCACCG AAGCCTGGAG 
GACCGGGTCA GCGCCGCGCG CGTACTGCAG CAAGGGGCTC CAGAATGCGC TCTCGTGCTC 
GACACGATGA CCAACTCCAG CAGCTCGGCC TACGGTGCCT ACTTCGAGCG CCTCTACATC 
ATCCAGAGTG GCACCATTAT GTACCAGGGC GGCCGCGGCC CCGACGGCTA CCAGGTCTCC 
GAGGTGCGCA CCTGGCTGGA GCGCTATGAT GAGCAGCTGC ACGGCCCACA GCCCCGTCGA 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010993.2
CDS	141..1046
Translation	MSRQAAPRWVVGEGRGTLGGAATMLRSLLLHSLRLCSQTASCLVLFPRFLGTAFMLWLLDFLCIRKHLLGRRRRGQPEIEVELNSDGEEVPPDDPPVCVSDDNRLCTLASLRAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQNQHILDYARGNRPLVLNFGSCT*PPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYSIPQHRSLEDRVSAARVLQQGAPECALVLDTMTNSSSSAYGAYFERLYIIQSGTIMYQGGRGPDGYQVSEVRTWLERYDEQLHGPQPRRV

Target ORF information:

RefSeq Version	NM_001010993.3
Organism	Bos taurus(cattle)
Definition	Bos taurus iodothyronine deiodinase 3 (DIO3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010993.3

ORF Insert Sequence:

ATGTCTCGCC AGGCTGCCCC GCGGTGGGTG GTGGGGGAAG GTAGGGGGAC TTTGGGGGGC 
GCCGCCACCA TGCTGCGCTC CCTGCTGCTT CACTCCCTGA GGCTCTGCTC CCAGACGGCC 
TCGTGCCTCG TGCTCTTCCC GCGCTTCCTG GGCACGGCCT TCATGCTCTG GCTGCTAGAC 
TTCCTGTGCA TCCGTAAGCA TTTACTGGGC CGTCGACGCC GGGGTCAGCC GGAAATCGAA 
GTGGAGCTCA ACAGCGATGG CGAGGAGGTG CCCCCCGACG ACCCGCCTGT CTGCGTGTCC 
GACGACAACC GCCTGTGCAC CTTGGCGTCG CTGAGGGCCG TCTGGCACGG CCAGAAGTTG 
GATTTCTTCA AGCAGGCGCA TGAAGGCGGG CCGGCGCCCA ACTCTGAGGT GGTCCTGCCC 
GACGGCTTCC AGAACCAGCA CATCCTCGAC TACGCCCGAG GAAACCGTCC GCTGGTGCTC 
AACTTCGGCA GCTGCACCTG ACCACCGTTC ATGGCGCGCA TGAGCGCCTT CCAGCGCCTG 
GTCACCAAGT ATCAGCGCGA CGTCGACTTC CTCATCATCT ACATCGAGGA AGCTCACCCT 
TCCGACGGCT GGGTCACCAC AGACTCTCCC TACAGCATCC CGCAGCACCG AAGCCTGGAG 
GACCGGGTCA GCGCCGCGCG CGTACTGCAG CAAGGGGCTC CAGAATGCGC TCTCGTGCTC 
GACACGATGA CCAACTCCAG CAGCTCGGCC TACGGTGCCT ACTTCGAGCG CCTCTACATC 
ATCCAGAGTG GCACCATTAT GTACCAGGGC GGCCGCGGCC CCGACGGCTA CCAGGTCTCC 
GAGGTGCGCA CCTGGCTGGA GCGCTATGAT GAGCAGCTGC ACGGCCCACA GCCCCGTCGA 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and imprinting of DIO3 and DIO3OS genes in Holstein cattle.
Journal of genetics96(2)333-339(2017 Jun)
Yang W,Li D,Wang G,Wu X,Zhang M,Zhang C,Cui Y,Li S

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Molecular cloning, expression and radiation hybrid mapping of the bovine deiodinase type II (DIO2) and deiodinase type III (DIO3) genes.
Animal genetics36(3)240-3(2005 Jun)
Connor EE,Laiakis EC,Fernandes VM,Williams JL,Capuco AV

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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DIO3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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