dio3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dio3a
Entrez Gene ID	100538339
Full Name	iodothyronine deiodinase 3a
General protein information	Preferred Names iodothyronine deiodinase 3a Names type IIIa iodothyronine deiodinase deiodinase, iodothyronine, type IIIa
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. Two paralogs of this gene have been identified in zebrafish on chromosomes 17 and 20. Both encode enzymatically active proteins; however, their gene structure and tissue expression patterns are markedly different. This gene is intronless, like orthologs in all other vertebrate species studied to date, but unlike the paralog on chromosome 20, which is 2-exon. Knockdown of this gene indicates that it is essential for normal zebrafish embryonic and early larval development. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256003.1	NP_001242932.1	type IIIa iodothyronine deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO3	NP_001353.4
Rattus norvegicus (Norway rat)	Dio3	NP_058906.3
Gallus gallus (chicken)	DIO3	NP_001116120.1
Xenopus tropicalis (tropical clawed frog)	dio3	NP_001107139.2
	DIO3	NP_001116121.1
Canis lupus familiaris (dog)	DIO3	NP_001157660.1
Danio rerio (zebrafish)	dio3a	NP_001242932.1
Bos taurus (cattle)	DIO3	NP_001010993.2
Mus musculus (house mouse)	Dio3	NP_742117.2
Danio rerio (zebrafish)	dio3b	NP_001171406.2

Gene Ontology
Bibliography

Related articles in PubMed

The Thyroid Hormone Inactivating Type 3 Deiodinase Is Essential for Optimal Neutrophil Function: Observations From Three Species.
van der Spek AH, Jim KK, Karaczyn A, van Beeren HC, Ackermans MT, Darras VM, Vandenbroucke-Grauls CMJE, Hernandez A, Brouwer MC, Fliers E, van de Beek D, Boelen A
Endocrinology159(2)826-835(2018 Feb)

In vivo Functional Consequences of Human THRA Variants Expressed in the Zebrafish.
Marelli F, Carra S, Rurale G, Cotelli F, Persani L
Thyroid : official journal of the American Thyroid Association27(2)279-291(2017 Feb)

Permanent Deiodinase Type 2 Deficiency Strongly Perturbs Zebrafish Development, Growth, and Fertility.
Houbrechts AM, Delarue J, Gabri?ls IJ, Sourbron J, Darras VM
Endocrinology157(9)3668-81(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dio3 expression controls zebrafish embryo size.
Title: Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size.

The following dio3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dio3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01560	NM_001256003.1 Latest version!	Danio rerio iodothyronine deiodinase 3a (dio3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dio3a ( NM_001256003.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001242932.1 Danio rerio iodothyronine deiodinase 3a (dio3a), mRNA.

Price & Availability↑

CloneID	ODa01560
Clone ID Related Accession (Same CDS sequence)	NM_001256003.1
Accession Version	NM_001256003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Danio rerio(zebrafish)
Product	type IIIa iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE557694.1, EH432430.1 and EH458785.1. ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACACCG GCAGGGCGCT GAAGAACGCG CTCGTGTGTC TGCTCATTCT CCCGCGGTTT 
CTGGTCGCCG CCTTCATGCT GTGGTGTCTG GATTTCCTGT GCGTGCGGAA GAGAGTTCTG 
GTCCACCTGC AGGAGCGCGC GGAGGAGGAA GAGGAGGACG CGGAGGAGGA AGAGGAGCCG 
CTCTGCATCT CCGACAGCAG CCGCATGTTC AGCTGGGAGT CTCTGAAAGC CGTGTTCCAC 
GGACACAAGC TGGACTACAT GAAGAGCGCG CGCCTCGGAC ACGCGGCGCC CGACAGCGAG 
GTGTTCCCGC TGGCGGAGCC GCGGCGCGGG CGCGTGCTGG AGTTTGCGCG CGGTCACCGG 
CCGCTGGTGC TGAGCTTCGG GAGCTGCAGC TGACCGCCGT TCATGAGGCG TCTGAAGGCG 
TTCCGGAGGC TCGTGCTGCG CTACGCGGAC GTGGCGGACG CGCTGCTGAT CTACATCGAG 
GAGGCGCACC CGAGCGACGG CTGGCGGAGC TCCGACGCGC CGCATCAGAT CCGCCGCCAC 
CGCAGTCTGG AGGAGCGGCT GAGCGCGGCG CGGCTGATGG AGCGCGAGGC GCCGGGCTGC 
GCGGTGGTGG CGGACGGCAT GGAGAACGCG GCCAACAGCG CGTACGGAGC TTACTTCGAC 
AGACTGTACA TCGTGCAGGA CGGGAGGGTG GTGTACCAGG GCGGCCGCGG ACCCGAGGGA 
TTCCGCATCT CCGAGCTCCG ACACTGGCTG GACCGGTACC GCGAGCGGCT GCGCGACGCC 
GTCATCCCGG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001242932.1
CDS	155..949
Translation	MNTGRALKNALVCLLILPRFLVAAFMLWCLDFLCVRKRVLVHLQERAEEEEEDAEEEEEPLCISDSSRMFSWESLKAVFHGHKLDYMKSARLGHAAPDSEVFPLAEPRRGRVLEFARGHRPLVLSFGSCS*PPFMRRLKAFRRLVLRYADVADALLIYIEEAHPSDGWRSSDAPHQIRRHRSLEERLSAARLMEREAPGCAVVADGMENAANSAYGAYFDRLYIVQDGRVVYQGGRGPEGFRISELRHWLDRYRERLRDAVIPV

Target ORF information:

RefSeq Version	NM_001256003.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio iodothyronine deiodinase 3a (dio3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256003.1

ORF Insert Sequence:

ATGAACACCG GCAGGGCGCT GAAGAACGCG CTCGTGTGTC TGCTCATTCT CCCGCGGTTT 
CTGGTCGCCG CCTTCATGCT GTGGTGTCTG GATTTCCTGT GCGTGCGGAA GAGAGTTCTG 
GTCCACCTGC AGGAGCGCGC GGAGGAGGAA GAGGAGGACG CGGAGGAGGA AGAGGAGCCG 
CTCTGCATCT CCGACAGCAG CCGCATGTTC AGCTGGGAGT CTCTGAAAGC CGTGTTCCAC 
GGACACAAGC TGGACTACAT GAAGAGCGCG CGCCTCGGAC ACGCGGCGCC CGACAGCGAG 
GTGTTCCCGC TGGCGGAGCC GCGGCGCGGG CGCGTGCTGG AGTTTGCGCG CGGTCACCGG 
CCGCTGGTGC TGAGCTTCGG GAGCTGCAGC TGACCGCCGT TCATGAGGCG TCTGAAGGCG 
TTCCGGAGGC TCGTGCTGCG CTACGCGGAC GTGGCGGACG CGCTGCTGAT CTACATCGAG 
GAGGCGCACC CGAGCGACGG CTGGCGGAGC TCCGACGCGC CGCATCAGAT CCGCCGCCAC 
CGCAGTCTGG AGGAGCGGCT GAGCGCGGCG CGGCTGATGG AGCGCGAGGC GCCGGGCTGC 
GCGGTGGTGG CGGACGGCAT GGAGAACGCG GCCAACAGCG CGTACGGAGC TTACTTCGAC 
AGACTGTACA TCGTGCAGGA CGGGAGGGTG GTGTACCAGG GCGGCCGCGG ACCCGAGGGA 
TTCCGCATCT CCGAGCTCCG ACACTGGCTG GACCGGTACC GCGAGCGGCT GCGCGACGCC 
GTCATCCCGG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Thyroid Hormone Inactivating Type 3 Deiodinase Is Essential for Optimal Neutrophil Function: Observations From Three Species.
Endocrinology159(2)826-835(2018 Feb)
van der Spek AH,Jim KK,Karaczyn A,van Beeren HC,Ackermans MT,Darras VM,Vandenbroucke-Grauls CMJE,Hernandez A,Brouwer MC,Fliers E,van de Beek D,Boelen A

In vivo Functional Consequences of Human THRA Variants Expressed in the Zebrafish.
Thyroid : official journal of the American Thyroid Association27(2)279-291(2017 Feb)
Marelli F,Carra S,Rurale G,Cotelli F,Persani L

Permanent Deiodinase Type 2 Deficiency Strongly Perturbs Zebrafish Development, Growth, and Fertility.
Endocrinology157(9)3668-81(2016 Sep)
Houbrechts AM,Delarue J,Gabri?ls IJ,Sourbron J,Darras VM

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

dio3a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報