dio3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dio3
Entrez Gene ID	100038242
Full Name	deiodinase, iodothyronine, type 3
Synonyms	5DIII,D3,DI-3,dioiii,txdi3
General protein information	Preferred Names deiodinase, iodothyronine, type 3 Names thyroxine 5-deiodinase gene 15 iodothyronine 5-deiodinase type III type 3 deiodinase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)
Summary	The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. As in other verterbrates, studies in xenopus show that this enzyme is involved in early embryonic development. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113667.2	NP_001107139.2	thyroxine 5-deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO3	NP_001353.4
Rattus norvegicus (Norway rat)	Dio3	NP_058906.3
Gallus gallus (chicken)	DIO3	NP_001116120.1
Xenopus tropicalis (tropical clawed frog)	dio3	NP_001107139.2
	DIO3	NP_001116121.1
Canis lupus familiaris (dog)	DIO3	NP_001157660.1
Danio rerio (zebrafish)	dio3a	NP_001242932.1
Bos taurus (cattle)	DIO3	NP_001010993.2
Mus musculus (house mouse)	Dio3	NP_742117.2
Danio rerio (zebrafish)	dio3b	NP_001171406.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of enzymes involved in thyroid hormone metabolism during the early development of Xenopus tropicalis.
Tindall AJ, Morris ID, Pownall ME, Isaacs HV
Biology of the cell99(3)151-63(2007 Mar)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dio3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dio3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa01412	NM_001113667.2 Latest version!	Xenopus tropicalis deiodinase, iodothyronine, type 3 (dio3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dio3 ( NM_001113667.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001107139.2 Xenopus tropicalis deiodinase, iodothyronine, type 3 (dio3), mRNA.

Price & Availability↑

CloneID	OXa01412
Clone ID Related Accession (Same CDS sequence)	NM_001113667.2
Accession Version	NM_001113667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-08
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thyroxine 5-deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from ES676781.1, BC157248.1 and DN068725.1. On Mar 7, 2012 this sequence version replaced NM_001113667.1. ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGCACT GTGCGGGACC CCACACCAGC AAACTTCTCA AACAGGTGGC CGCCTGCTGC 
CTGCTGCTGC CCCGCTTCCT GCTCACGGCG CTGATGCTGT GGCTGCTGGA TTTCCAGTGC 
ATCAGGAGGA GGGTCCTGGT AACCGCCAGG GAGGAGAGCA GCCCGGAGCT GGAGGACCCC 
CCGCTGTGCG TGTCCGACTC CAACCGTATG TGCACAGTGG AGTCGCTGCG AGCGGTCTGG 
CACGGACAGA AGCTGGACTA CTTCAAGTCT GCGCACCTGG GCAGCTCTGC CCCCAACACT 
GAGGTGGTGA TGCTGGAAGG GCGCAGGCTG TGCAGGATCC TGGATTTCTC CCAGGGCAAG 
AGACCGCTGG TTGTCAATTT CGGCAGCTGC ACCTGACCCC CATTCATGGC TCGCCTGCAA 
GCCTACCGCC GCCTGGCAGC CCAACACGTC GGCACGGCAG ATTTCCTGCT GGTCTATATA 
GAGGAAGCGC ACCCGTCCGA CGGCTGGGTC AGCACCGACG CCTCCTACCA AATCCCCCAG 
CACCAGTGCC TGCAGGACCG CTTGGCGGCG GCTCAGCTCA TGCTCCAAGG TGTGCCGGGC 
TGCAGGGTGG TGGCGGACAC CATGGACAAT TCATCCAACG CCGCCTACGG TGCCTACTTT 
GAGAGACTTT ACATTATCCT GGAGGGCAAG GTGGTGTACC AGGGAGGGCG GGGGCCGGAA 
GGCTACAAGA TCTCGGAACT GAGGATGTGG TTGGAGCAGT ACCAGCAGGG CACGATTGGT 
ACCTCGGGCA CCGGCCATGT GGTCATCCAA GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107139.2
CDS	32..847
Translation	MLHCAGPHTSKLLKQVAACCLLLPRFLLTALMLWLLDFQCIRRRVLVTAREESSPELEDPPLCVSDSNRMCTVESLRAVWHGQKLDYFKSAHLGSSAPNTEVVMLEGRRLCRILDFSQGKRPLVVNFGSCT*PPFMARLQAYRRLAAQHVGTADFLLVYIEEAHPSDGWVSTDASYQIPQHQCLQDRLAAAQLMLQGVPGCRVVADTMDNSSNAAYGAYFERLYIILEGKVVYQGGRGPEGYKISELRMWLEQYQQGTIGTSGTGHVVIQV

Target ORF information:

RefSeq Version	NM_001113667.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis deiodinase, iodothyronine, type 3 (dio3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113667.2

ORF Insert Sequence:

ATGTTGCACT GTGCGGGACC CCACACCAGC AAACTTCTCA AACAGGTGGC CGCCTGCTGC 
CTGCTGCTGC CCCGCTTCCT GCTCACGGCG CTGATGCTGT GGCTGCTGGA TTTCCAGTGC 
ATCAGGAGGA GGGTCCTGGT AACCGCCAGG GAGGAGAGCA GCCCGGAGCT GGAGGACCCC 
CCGCTGTGCG TGTCCGACTC CAACCGTATG TGCACAGTGG AGTCGCTGCG AGCGGTCTGG 
CACGGACAGA AGCTGGACTA CTTCAAGTCT GCGCACCTGG GCAGCTCTGC CCCCAACACT 
GAGGTGGTGA TGCTGGAAGG GCGCAGGCTG TGCAGGATCC TGGATTTCTC CCAGGGCAAG 
AGACCGCTGG TTGTCAATTT CGGCAGCTGC ACCTGACCCC CATTCATGGC TCGCCTGCAA 
GCCTACCGCC GCCTGGCAGC CCAACACGTC GGCACGGCAG ATTTCCTGCT GGTCTATATA 
GAGGAAGCGC ACCCGTCCGA CGGCTGGGTC AGCACCGACG CCTCCTACCA AATCCCCCAG 
CACCAGTGCC TGCAGGACCG CTTGGCGGCG GCTCAGCTCA TGCTCCAAGG TGTGCCGGGC 
TGCAGGGTGG TGGCGGACAC CATGGACAAT TCATCCAACG CCGCCTACGG TGCCTACTTT 
GAGAGACTTT ACATTATCCT GGAGGGCAAG GTGGTGTACC AGGGAGGGCG GGGGCCGGAA 
GGCTACAAGA TCTCGGAACT GAGGATGTGG TTGGAGCAGT ACCAGCAGGG CACGATTGGT 
ACCTCGGGCA CCGGCCATGT GGTCATCCAA GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of enzymes involved in thyroid hormone metabolism during the early development of Xenopus tropicalis.
Biology of the cell99(3)151-63(2007 Mar)
Tindall AJ,Morris ID,Pownall ME,Isaacs HV

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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dio3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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