dio3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dio3b
Entrez Gene ID	798872
Full Name	iodothyronine deiodinase 3b
Synonyms	dio3,si:ch211-156m19.1
General protein information	Preferred Names iodothyronine deiodinase 3b Names type IIIb iodothyronine deiodinase D3 deiodinase 3 deiodinase, iodothyronine type IIIb deiodinase, iodothyronine, type III iodothyronine type IIIb type III iodothyronine deiodinase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. Two paralogs of this gene have been identified in zebrafish on chromosomes 17 and 20. Both encode enzymatically active proteins; however, their gene structure and tissue expression patterns are markedly different. This gene is unique in that it has 2 exons compared to orthologs in all other vertebrate species studied to date, which are intronless. Knockdown of this gene causes severe microsomia, indicating that this gene is critical for the determination fetal size during early embryonic development. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001177935.3	NP_001171406.2	type IIIb iodothyronine deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO3	NP_001353.4
Rattus norvegicus (Norway rat)	Dio3	NP_058906.3
Gallus gallus (chicken)	DIO3	NP_001116120.1
Xenopus tropicalis (tropical clawed frog)	dio3	NP_001107139.2
	DIO3	NP_001116121.1
Canis lupus familiaris (dog)	DIO3	NP_001157660.1
Danio rerio (zebrafish)	dio3a	NP_001242932.1
Bos taurus (cattle)	DIO3	NP_001010993.2
Mus musculus (house mouse)	Dio3	NP_742117.2
Danio rerio (zebrafish)	dio3b	NP_001171406.2

Gene Ontology
Bibliography

Related articles in PubMed

Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size.
Guo C, Chen X, Song H, Maynard MA, Zhou Y, Lobanov AV, Gladyshev VN, Ganis JJ, Wiley D, Jugo RH, Lee NY, Castroneves LA, Zon LI, Scanlan TS, Feldman HA, Huang SA
Endocrinology155(10)4069-80(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of D1+D2 (D1D2MO) and knockdown of D3 (D3MO) both resulted in transcriptional regulation of energy metabolism and (muscle) development in abdomen and tail
Title: Deiodinase knockdown during early zebrafish development affects growth, development, energy metabolism, motility and phototransduction.

Dio3 expression controls zebrafish embryo size.
Title: Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size.

D3 is essential for normal zebrafish embryonic and early larval development
Title: Knockdown of type 3 iodothyronine deiodinase severely perturbs both embryonic and early larval development in zebrafish.

This study investigated deiodinase 1 (Dio1), deiodinase 2 (Dio2), and deiodinase 3 (Dio3) mRNA expression at the several zebrafish life stages and found life stage specific expression of these genes that were highly localized.
Title: Using whole mount in situ hybridization to examine thyroid hormone deiodinase expression in embryonic and larval zebrafish: a tool for examining OH-BDE toxicity to early life stages.

The following dio3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dio3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02049	NM_001177935.3 Latest version!	Danio rerio iodothyronine deiodinase 3b (dio3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dio3b ( NM_001177935.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001171406.2 Danio rerio iodothyronine deiodinase 3b (dio3b), mRNA.

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CloneID	ODa02049
Clone ID Related Accession (Same CDS sequence)	NM_001177935.3
Accession Version	NM_001177935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	type IIIb iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KM281667.1. On Jul 1, 2016 this sequence version replaced NM_001177935.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KM281666.1, KM281667.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 25004091 protein contains selenocysteine :: PMID: 25004091 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGATGC TGCAGGGCTC CGCAGGTGTG CAGTCTGCGC TGAAGAACGC GGCGGTGTGT 
GTGCTGCTGC TCCCGCGCTT TCTGCTCGCC GCGCTGATGC TCTGCCTGCT GGACTTCCTG 
TGCATCCGCC GGAAGCTGCT GCTGAAGATG CAGGAGGGCG CGTTCAGCAG CCCCGACGAC 
CCTCCGCTGC GCGTGTCCGA CAGCAACAAG ATGTTCACGC TGGAGTCGCT GCGCGCGGTC 
TGGTACGGCC AGAAGCTGGA CTTCTTCAAG AGCGCGCGGC TCGGCGGAGC GGCGCCCAAC 
ACCGAGGTGT TCCCGCTGGA CGGAGACGCG CGCGCGGCAG AGCGCATCCT GGATTACGCG 
CGCGGCCGGA GACCGCTGAT CCTCAACTTC GGCAGCTGCT CCTGACCGCC CTTCATGACC 
CGTCTGAGCG CCTTCCAGCG CGTCGCGCGG CAGTACGCGG ACATCGCGGA CTCTCTGCTG 
GTGTACATCG AGGAGGCGCA CCCGTCCGAC GGCTGGGTGA GCTCCGACGC GCCCGTGCAG 
ATCCCGCGGC ACCGCTGCCT GGAGGACCGG CTCCGCGCCG CGCAGATGCT GCACCGGGAG 
GCGGCGGGCA GCGCGGTGGT CGTGGACTCC ATGCAGAACT CCTGCAACGC GGCGTACGGC 
GCGTATTTCG AGCGGCTGTA CATCGTAAAG GACGCGACGG TGGTGTACCA AGGCGGCCGC 
GGACCCGAGG GGTACCGGAT CGCGGAGCTC AGAGACTGGC TGGAGCGCTA CAGGAGCGGC 
CTGCAGGAGT CCGCGGTGCT GCACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171406.2
CDS	94..903
Translation	MEMLQGSAGVQSALKNAAVCVLLLPRFLLAALMLCLLDFLCIRRKLLLKMQEGAFSSPDDPPLRVSDSNKMFTLESLRAVWYGQKLDFFKSARLGGAAPNTEVFPLDGDARAAERILDYARGRRPLILNFGSCS*PPFMTRLSAFQRVARQYADIADSLLVYIEEAHPSDGWVSSDAPVQIPRHRCLEDRLRAAQMLHREAAGSAVVVDSMQNSCNAAYGAYFERLYIVKDATVVYQGGRGPEGYRIAELRDWLERYRSGLQESAVLHV

Target ORF information:

RefSeq Version	NM_001177935.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio iodothyronine deiodinase 3b (dio3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177935.3

ORF Insert Sequence:

ATGGAGATGC TGCAGGGCTC CGCAGGTGTG CAGTCTGCGC TGAAGAACGC GGCGGTGTGT 
GTGCTGCTGC TCCCGCGCTT TCTGCTCGCC GCGCTGATGC TCTGCCTGCT GGACTTCCTG 
TGCATCCGCC GGAAGCTGCT GCTGAAGATG CAGGAGGGCG CGTTCAGCAG CCCCGACGAC 
CCTCCGCTGC GCGTGTCCGA CAGCAACAAG ATGTTCACGC TGGAGTCGCT GCGCGCGGTC 
TGGTACGGCC AGAAGCTGGA CTTCTTCAAG AGCGCGCGGC TCGGCGGAGC GGCGCCCAAC 
ACCGAGGTGT TCCCGCTGGA CGGAGACGCG CGCGCGGCAG AGCGCATCCT GGATTACGCG 
CGCGGCCGGA GACCGCTGAT CCTCAACTTC GGCAGCTGCT CCTGACCGCC CTTCATGACC 
CGTCTGAGCG CCTTCCAGCG CGTCGCGCGG CAGTACGCGG ACATCGCGGA CTCTCTGCTG 
GTGTACATCG AGGAGGCGCA CCCGTCCGAC GGCTGGGTGA GCTCCGACGC GCCCGTGCAG 
ATCCCGCGGC ACCGCTGCCT GGAGGACCGG CTCCGCGCCG CGCAGATGCT GCACCGGGAG 
GCGGCGGGCA GCGCGGTGGT CGTGGACTCC ATGCAGAACT CCTGCAACGC GGCGTACGGC 
GCGTATTTCG AGCGGCTGTA CATCGTAAAG GACGCGACGG TGGTGTACCA AGGCGGCCGC 
GGACCCGAGG GGTACCGGAT CGCGGAGCTC AGAGACTGGC TGGAGCGCTA CAGGAGCGGC 
CTGCAGGAGT CCGCGGTGCT GCACGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size.
Endocrinology155(10)4069-80(2014 Oct)
Guo C,Chen X,Song H,Maynard MA,Zhou Y,Lobanov AV,Gladyshev VN,Ganis JJ,Wiley D,Jugo RH,Lee NY,Castroneves LA,Zon LI,Scanlan TS,Feldman HA,Huang SA

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dio3b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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