DIO3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIO3
Entrez Gene ID	395939
Full Name	iodothyronine deiodinase 3
General protein information	Preferred Names iodothyronine deiodinase 3 Names thyroxine 5-deiodinase 5DIII DIOIII deiodinase, iodothyronine type III type 3 DI type III iodothyronine deiodinase type-III 5'-deiodinase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)
Summary	The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. As in mammals, studies in chicken show that this enzyme is involved in embryonic development. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001122648.2	NP_001116120.1	thyroxine 5-deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO3	NP_001353.4
Rattus norvegicus (Norway rat)	Dio3	NP_058906.3
Gallus gallus (chicken)	DIO3	NP_001116120.1
Xenopus tropicalis (tropical clawed frog)	dio3	NP_001107139.2
	DIO3	NP_001116121.1
Canis lupus familiaris (dog)	DIO3	NP_001157660.1
Danio rerio (zebrafish)	dio3a	NP_001242932.1
Bos taurus (cattle)	DIO3	NP_001010993.2
Mus musculus (house mouse)	Dio3	NP_742117.2
Danio rerio (zebrafish)	dio3b	NP_001171406.2

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic mRNA distribution pattern of thyroid hormone transporters and deiodinases during early embryonic chicken brain development.
Geysens S, Ferran JL, Van Herck SL, Tylzanowski P, Puelles L, Darras VM
Neuroscience22169-85(2012 Sep)

Gene expression profiling of chicken primordial germ cell ESTs.
Han JY, Park TS, Kim JN, Kim MA, Lim D, Lim JM, Kim H
BMC genomics7220(2006 Aug)

A comprehensive collection of chicken cDNAs.
Boardman PE, Sanz-Ezquerro J, Overton IM, Burt DW, Bosch E, Fong WT, Tickle C, Brown WR, Wilson SA, Hubbard SJ
Current biology : CB12(22)1965-9(2002 Nov)

Expression of chicken hepatic type I and type III iodothyronine deiodinases during embryonic development.
Van der Geyten S, Sanders JP, Kaptein E, Darras VM, K?hn ER, Leonard JL, Visser TJ
Endocrinology138(12)5144-52(1997 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

D3 are expressed in the choroid plexus and several sensory brain centers
Title: Dynamic mRNA distribution pattern of thyroid hormone transporters and deiodinases during early embryonic chicken brain development.

The following DIO3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIO3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28228	NM_001122648.2 Latest version!	Gallus gallus deiodinase, iodothyronine type III (DIO3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIO3 ( NM_001122648.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001116120.1 Gallus gallus deiodinase, iodothyronine type III (DIO3), mRNA.

Price & Availability↑

CloneID	OGa28228
Clone ID Related Accession (Same CDS sequence)	NM_001122648.2
Accession Version	NM_001122648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Gallus gallus(chicken)
Product	thyroxine 5-deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000303.1, Y11273.1, DR413250.1 and CK608350.1. On Jun 28, 2016 this sequence version replaced NM_001122648.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCCACT CGCTGGGCGC TCACACCTTG CAGCTGCTCA CCCAGGCGGC CGCCTGCATC 
CTCCTCTTTC CCCGCTTCCT GCTCACCGCT GTGATGCTCT GGCTCCTGGA TTTTCTGTGC 
ATTCGCAAGA AGATGCTGAC GATGCCCACG GCGGAGGAGG CGGCCGGAGC CGGCGAGGGG 
CCGCCCCCCG ACGACCCTCC GGTCTGCGTG TCCGACTCCA ACCGCATGTT CACGCTGGAG 
TCGCTCAAGG CCGTGTGGCA CGGGCAGAAG CTGGACTTCT TCAAGTCGGC GCACGTGGGC 
TCGCCTGCCC CCAACCCCGA GGTGATCCAG CTGGACGGGC AGAAGAGGCT CCGCATCCTC 
GACTTCGCCC GCGGCAAGAG GCCCCTCATC CTCAACTTCG GCAGCTGCAC CTGACCCCCG 
TTCATGGCCC GCCTGAGGTC CTTCCGGCGC CTGGCCGCGC ACTTCGTGGA CATTGCCGAC 
TTCCTGCTGG TGTACATCGA AGAAGCGCAC CCCTCCGACG GCTGGGTCAG CTCGGACGCT 
GCCTACAGCA TCCCCAAGCA CCAGTGCCTC CAGGACAGGC TGCGGGCGGC GCAGCTGATG 
CGGGAAGGGG CGCCCGATTG CCCCCTGGCC GTGGACACCA TGGACAACGC TTCCAGCGCT 
GCCTACGGCG CCTACTTCGA GCGGCTCTAC GTCATCCAGG AGGAGAAGGT GATGTACCAG 
GGCGGCCGAG GACCGGAGGG CTACAAGATC TCGGAGCTGC GGAGCTGGCT AGACCAGTAC 
AAAACCCGGC TCCAGAGCCC CGGCGCGGTG GTCATCCAAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116120.1
CDS	100..924
Translation	MLHSLGAHTLQLLTQAAACILLFPRFLLTAVMLWLLDFLCIRKKMLTMPTAEEAAGAGEGPPPDDPPVCVSDSNRMFTLESLKAVWHGQKLDFFKSAHVGSPAPNPEVIQLDGQKRLRILDFARGKRPLILNFGSCT*PPFMARLRSFRRLAAHFVDIADFLLVYIEEAHPSDGWVSSDAAYSIPKHQCLQDRLRAAQLMREGAPDCPLAVDTMDNASSAAYGAYFERLYVIQEEKVMYQGGRGPEGYKISELRSWLDQYKTRLQSPGAVVIQV

Target ORF information:

RefSeq Version	NM_001122648.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus deiodinase, iodothyronine type III (DIO3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122648.2

ORF Insert Sequence:

ATGCTCCACT CGCTGGGCGC TCACACCTTG CAGCTGCTCA CCCAGGCGGC CGCCTGCATC 
CTCCTCTTTC CCCGCTTCCT GCTCACCGCT GTGATGCTCT GGCTCCTGGA TTTTCTGTGC 
ATTCGCAAGA AGATGCTGAC GATGCCCACG GCGGAGGAGG CGGCCGGAGC CGGCGAGGGG 
CCGCCCCCCG ACGACCCTCC GGTCTGCGTG TCCGACTCCA ACCGCATGTT CACGCTGGAG 
TCGCTCAAGG CCGTGTGGCA CGGGCAGAAG CTGGACTTCT TCAAGTCGGC GCACGTGGGC 
TCGCCTGCCC CCAACCCCGA GGTGATCCAG CTGGACGGGC AGAAGAGGCT CCGCATCCTC 
GACTTCGCCC GCGGCAAGAG GCCCCTCATC CTCAACTTCG GCAGCTGCAC CTGACCCCCG 
TTCATGGCCC GCCTGAGGTC CTTCCGGCGC CTGGCCGCGC ACTTCGTGGA CATTGCCGAC 
TTCCTGCTGG TGTACATCGA AGAAGCGCAC CCCTCCGACG GCTGGGTCAG CTCGGACGCT 
GCCTACAGCA TCCCCAAGCA CCAGTGCCTC CAGGACAGGC TGCGGGCGGC GCAGCTGATG 
CGGGAAGGGG CGCCCGATTG CCCCCTGGCC GTGGACACCA TGGACAACGC TTCCAGCGCT 
GCCTACGGCG CCTACTTCGA GCGGCTCTAC GTCATCCAGG AGGAGAAGGT GATGTACCAG 
GGCGGCCGAG GACCGGAGGG CTACAAGATC TCGGAGCTGC GGAGCTGGCT AGACCAGTAC 
AAAACCCGGC TCCAGAGCCC CGGCGCGGTG GTCATCCAAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic mRNA distribution pattern of thyroid hormone transporters and deiodinases during early embryonic chicken brain development.
Neuroscience22169-85(2012 Sep)
Geysens S,Ferran JL,Van Herck SL,Tylzanowski P,Puelles L,Darras VM

Gene expression profiling of chicken primordial germ cell ESTs.
BMC genomics7220(2006 Aug)
Han JY,Park TS,Kim JN,Kim MA,Lim D,Lim JM,Kim H

A comprehensive collection of chicken cDNAs.
Current biology : CB12(22)1965-9(2002 Nov)
Boardman PE,Sanz-Ezquerro J,Overton IM,Burt DW,Bosch E,Fong WT,Tickle C,Brown WR,Wilson SA,Hubbard SJ

Expression of chicken hepatic type I and type III iodothyronine deiodinases during embryonic development.
Endocrinology138(12)5144-52(1997 Dec)
Van der Geyten S,Sanders JP,Kaptein E,Darras VM,K?hn ER,Leonard JL,Visser TJ

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DIO3 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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