ORF » Species Summary » Bos taurus » CAST cDNA ORF clone

CAST cDNA ORF clone, Bos taurus(cattle)

The following CAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa95429 NM_001030320.3
Latest version!		 Bos taurus calpastatin (CAST), transcript variant IV, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa21777 XM_005209751.2
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $426.30
$609.00
OBa21780 XM_005209754.3
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $426.30
$609.00
OBa21778 NM_001030319.4
Latest version!		 Bos taurus calpastatin (CAST), transcript variant III, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21778 XM_010807619.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21778 XM_005209752.2
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21778 XM_024993826.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21779 XM_010807620.2
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21779 XM_005209753.2
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21779 XM_024993828.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa41352 NM_174003.2
Latest version!		 Bos taurus calpastatin (CAST), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa235758 XM_024993825.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa235759 XM_024993827.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa21775 NM_001030318.3
Latest version!		 Bos taurus calpastatin (CAST), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa21775 XM_005209749.3
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa21775 XM_024993823.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa21775 XM_024993824.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa21776 XM_005209750.3
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa45055 XM_010807616.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa45056 XM_010807617.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa45057 XM_010807618.1
Latest version!		 Bos taurus calpastatin (CAST), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
View Old Accession Versions >>
OBa21775 XM_005209749.2 Bos taurus calpastatin (CAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa21776 XM_005209750.2 Bos taurus calpastatin (CAST), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa21777 XM_005209751.1 Bos taurus calpastatin (CAST), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $426.30
$609.00
OBa21779 XM_005209753.1 Bos taurus calpastatin (CAST), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa21778 XM_005209752.1 Bos taurus calpastatin (CAST), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CAST ( NM_001030320.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001025491.2 Bos taurus calpastatin (CAST), transcript variant IV, mRNA.
    Price & Availability↑
    CloneID OBa95429
    Clone ID Related Accession (Same CDS sequence) NM_001030320.3
    Accession Version NM_001030320.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Bos taurus(cattle)
    Product calpastatin isoform IV
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On Jul 2, 2016 this sequence version replaced NM_001030320.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY834764.1, SRR5571257.32766.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145491, SAMN03145497 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCAGT TTCTATCGTC AACCTTTTTG GAGGACTCAC TGGGTGCCGT ATGGCACGAA 
    AGGCTTTACG AAGGCGAAGG CAAGGGAGCG GGAGAAGCCG CCTTTGTCCT CCAGGACCAA 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001025491.2
    CDS1..1317
    Translation

    Target ORF information:

    RefSeq Version NM_001030320.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant IV, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001030320.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCAGT TTCTATCGTC AACCTTTTTG GAGGACTCAC TGGGTGCCGT ATGGCACGAA 
    AGGCTTTACG AAGGCGAAGG CAAGGGAGCG GGAGAAGCCG CCTTTGTCCT CCAGGACCAA 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209751.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209808.1 Bos taurus calpastatin (CAST), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OBa21777
    Clone ID Related Accession (Same CDS sequence) XM_005209751.1 , XM_005209751.2
    Accession Version XM_005209751.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product calpastatin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104062.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGATGAAAAA GCCCTGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTGT AAAAACAGAA 
    CCTGAGAAGA AGCCACAATC ATCTAAGCCA TCTGTGGTTC ATGAGAAAAA AACCCAAGAA 
    GTAAAGCCAA AGGAACACAC AGAGCCAAAA AGCCTACCCA AGCACTCATC AGATACAGGA 
    AGCAAGCATG CTCCTAAGGA AAAAGCCGTT TCCAAATCAA GTGAGCAGCC ACCATCAGAG 
    AAATCAACAA AACCAAAGAC CAAGTCACAG GACAAGATCT CCGGTGGTGG AAAGAGCACT 
    GTTCCTGCTG CTGCTGCTGC AGCATCTGCC GAACCAGCTG ACAAGAATAA AGAAAATAAA 
    TTGTTAACAT CGGCCGTACC AGCTGAATCT AAACCAAGTA AACCATCTGG AAAGTCAGAC 
    ATGGACACTG CTCTGGATGA CTTAATAGAC ACTTTAGGAG AACCTGAAGA GATGAAAGAA 
    GATAACACAA CATATACCGG ACCGGAAGTG TCGGATCCAA TGAGTTCTAC CTACATAGAG 
    GAACTGGGTA AAAGAGAATC CACACTTCCT CCAAAATATA AGGAACTTCT GAATAAAGAA 
    GAAGGGATCG CAGGGCCTCC TCCAGACTCC TTGAAACCCC TGGGGCCCAA TGATGCCATC 
    GATGCCTTGT CATCCGACTT CACCTGCAGT TCCCCTACAG CTGATGCAAA GAAAACTGAG 
    AAAGAGAAAT CTACAGAAGA GGCTTTAAAA GCTCAGTCAG CTGGGGTGAT CAGAAGTGCT 
    GCTCCACCCC AAGAGAAAAA AAGGAAAGTG GAAAAGGATG CCATGACTGA GCACGCCCTG 
    GAGGCCCTGT CAGCCTCCCT GGGCACCCGG AAGCCGGAGC CGGAGCTCGA CCCCAGCTCC 
    ATTAAGGAGG TCGATGAGGC AAAAGCCAAA GAAGAGAAAG TAAAGAAATG TGGTGAAGAT 
    GAGGAAACAG TCCCATCGGA GTACAGATTA AAACCGGCCA CAGATAAAGA TGGAAAACCA 
    CTCTTGCCAG AGGCTGAAGA AAAACCCAAG CCCCTGAGTG AATCAGAACT CATCGATGAA 
    CTCTCAGAAG ATTTTGACCA GTCTAAGTGT AAAGAAAAAC AATCTAAGCC AACTGAAAAA 
    ACAGAGGCAT CCCCGGCCGC TGCCCCTGTA CCCGTGGCAG AGGACGTGCC TCGGACCTCT 
    ATGTGTTCCG TGCAGTCGGC TCCGCCCACA GCAGCTCCAG TGAAGGGCAT GGTGCCAGAC 
    GATGCTGTTG AAGCCTTGGC TGGAAGCCTG GGCAAAAAGG AAGCAGATCC AGAAGACGGA 
    AAGCCTGTGG AGGATAAAGT CAAGGAAAAA GCCAAAGAAG AGGATCGTGA GAAACTTGGT 
    GAAAAAGAAG AAACGATTCC TCCTGATTAC AGATTAGAAG AAGCCAAGGA TAAAGACGGA 
    AAACCACTGC TGCCAAAAGA GGTCAAGGAA CCGCTCCCAC CCTTGAGTGA AGACGTCCTC 
    CTCGATGCTC TGTCCAAGGA CTTCACTGTC CCCTCAGACA CATCATCGCC TCAATTTGAA 
    GATGCTAAAC TTTCAGCTGT CGTCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACC 
    CAGGCAGCCG GTCCACCCCC CAGCACTGCG CAGCGTGACA ACAAAGAACT TGACGATGCC 
    CTGGATCAAC TTTCTGACAG TCTCGGGCAA AGACAGCCTG ATCCAGATGA GAATAAACCC 
    GTAGAGGATA AAGTCAAGGA AAAAGCCAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
    GATGACACCA TCCCACCTAA ATACCAACAT CTTTTGGATG ACAACAAGGA GGGCACACCC 
    GGGAAGCCAA AGGCATCAGA GAAGCCCAAG GCATCAGAGA AACCTGCAGG TGCCCAGGAC 
    CCCATTGATG CCCTCTCAGG GGACTTTGAC AGCTGTCCCT CGACTACAGA AACCTCGACA 
    GACACACCAA AGGACAAAGA CAAGAAGCCT GCTTCCAGTG CCGAAGCACC TAGGAATGGC 
    GGGAAAGCAA AGGATTCCAC AAAGGCAAAG GAGGAAACTT CCAAGCCAAA AGCTGATGGA 
    AAAAGTACAA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209808.1
    CDS126..2420
    Translation

    Target ORF information:

    RefSeq Version XM_005209751.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGATGAAAAA GCCCTGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTGT AAAAACAGAA 
    CCTGAGAAGA AGCCACAATC ATCTAAGCCA TCTGTGGTTC ATGAGAAAAA AACCCAAGAA 
    GTAAAGCCAA AGGAACACAC AGAGCCAAAA AGCCTACCCA AGCACTCATC AGATACAGGA 
    AGCAAGCATG CTCCTAAGGA AAAAGCCGTT TCCAAATCAA GTGAGCAGCC ACCATCAGAG 
    AAATCAACAA AACCAAAGAC CAAGTCACAG GACAAGATCT CCGGTGGTGG AAAGAGCACT 
    GTTCCTGCTG CTGCTGCTGC AGCATCTGCC GAACCAGCTG ACAAGAATAA AGAAAATAAA 
    TTGTTAACAT CGGCCGTACC AGCTGAATCT AAACCAAGTA AACCATCTGG AAAGTCAGAC 
    ATGGACACTG CTCTGGATGA CTTAATAGAC ACTTTAGGAG AACCTGAAGA GATGAAAGAA 
    GATAACACAA CATATACCGG ACCGGAAGTG TCGGATCCAA TGAGTTCTAC CTACATAGAG 
    GAACTGGGTA AAAGAGAATC CACACTTCCT CCAAAATATA AGGAACTTCT GAATAAAGAA 
    GAAGGGATCG CAGGGCCTCC TCCAGACTCC TTGAAACCCC TGGGGCCCAA TGATGCCATC 
    GATGCCTTGT CATCCGACTT CACCTGCAGT TCCCCTACAG CTGATGCAAA GAAAACTGAG 
    AAAGAGAAAT CTACAGAAGA GGCTTTAAAA GCTCAGTCAG CTGGGGTGAT CAGAAGTGCT 
    GCTCCACCCC AAGAGAAAAA AAGGAAAGTG GAAAAGGATG CCATGACTGA GCACGCCCTG 
    GAGGCCCTGT CAGCCTCCCT GGGCACCCGG AAGCCGGAGC CGGAGCTCGA CCCCAGCTCC 
    ATTAAGGAGG TCGATGAGGC AAAAGCCAAA GAAGAGAAAG TAAAGAAATG TGGTGAAGAT 
    GAGGAAACAG TCCCATCGGA GTACAGATTA AAACCGGCCA CAGATAAAGA TGGAAAACCA 
    CTCTTGCCAG AGGCTGAAGA AAAACCCAAG CCCCTGAGTG AATCAGAACT CATCGATGAA 
    CTCTCAGAAG ATTTTGACCA GTCTAAGTGT AAAGAAAAAC AATCTAAGCC AACTGAAAAA 
    ACAGAGGCAT CCCCGGCCGC TGCCCCTGTA CCCGTGGCAG AGGACGTGCC TCGGACCTCT 
    ATGTGTTCCG TGCAGTCGGC TCCGCCCACA GCAGCTCCAG TGAAGGGCAT GGTGCCAGAC 
    GATGCTGTTG AAGCCTTGGC TGGAAGCCTG GGCAAAAAGG AAGCAGATCC AGAAGACGGA 
    AAGCCTGTGG AGGATAAAGT CAAGGAAAAA GCCAAAGAAG AGGATCGTGA GAAACTTGGT 
    GAAAAAGAAG AAACGATTCC TCCTGATTAC AGATTAGAAG AAGCCAAGGA TAAAGACGGA 
    AAACCACTGC TGCCAAAAGA GGTCAAGGAA CCGCTCCCAC CCTTGAGTGA AGACGTCCTC 
    CTCGATGCTC TGTCCAAGGA CTTCACTGTC CCCTCAGACA CATCATCGCC TCAATTTGAA 
    GATGCTAAAC TTTCAGCTGT CGTCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACC 
    CAGGCAGCCG GTCCACCCCC CAGCACTGCG CAGCGTGACA ACAAAGAACT TGACGATGCC 
    CTGGATCAAC TTTCTGACAG TCTCGGGCAA AGACAGCCTG ATCCAGATGA GAATAAACCC 
    GTAGAGGATA AAGTCAAGGA AAAAGCCAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
    GATGACACCA TCCCACCTAA ATACCAACAT CTTTTGGATG ACAACAAGGA GGGCACACCC 
    GGGAAGCCAA AGGCATCAGA GAAGCCCAAG GCATCAGAGA AACCTGCAGG TGCCCAGGAC 
    CCCATTGATG CCCTCTCAGG GGACTTTGAC AGCTGTCCCT CGACTACAGA AACCTCGACA 
    GACACACCAA AGGACAAAGA CAAGAAGCCT GCTTCCAGTG CCGAAGCACC TAGGAATGGC 
    GGGAAAGCAA AGGATTCCAC AAAGGCAAAG GAGGAAACTT CCAAGCCAAA AGCTGATGGA 
    AAAAGTACAA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209751.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209808.1 Bos taurus calpastatin (CAST), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OBa21777
    Clone ID Related Accession (Same CDS sequence) XM_005209751.1 , XM_005209751.2
    Accession Version XM_005209751.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGATGAAAAA GCCCTGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTGT AAAAACAGAA 
    CCTGAGAAGA AGCCACAATC ATCTAAGCCA TCTGTGGTTC ATGAGAAAAA AACCCAAGAA 
    GTAAAGCCAA AGGAACACAC AGAGCCAAAA AGCCTACCCA AGCACTCATC AGATACAGGA 
    AGCAAGCATG CTCCTAAGGA AAAAGCCGTT TCCAAATCAA GTGAGCAGCC ACCATCAGAG 
    AAATCAACAA AACCAAAGAC CAAGTCACAG GACAAGATCT CCGGTGGTGG AAAGAGCACT 
    GTTCCTGCTG CTGCTGCTGC AGCATCTGCC GAACCAGCTG ACAAGAATAA AGAAAATAAA 
    TTGTTAACAT CGGCCGTACC AGCTGAATCT AAACCAAGTA AACCATCTGG AAAGTCAGAC 
    ATGGACACTG CTCTGGATGA CTTAATAGAC ACTTTAGGAG AACCTGAAGA GATGAAAGAA 
    GATAACACAA CATATACCGG ACCGGAAGTG TCGGATCCAA TGAGTTCTAC CTACATAGAG 
    GAACTGGGTA AAAGAGAATC CACACTTCCT CCAAAATATA AGGAACTTCT GAATAAAGAA 
    GAAGGGATCG CAGGGCCTCC TCCAGACTCC TTGAAACCCC TGGGGCCCAA TGATGCCATC 
    GATGCCTTGT CATCCGACTT CACCTGCAGT TCCCCTACAG CTGATGCAAA GAAAACTGAG 
    AAAGAGAAAT CTACAGAAGA GGCTTTAAAA GCTCAGTCAG CTGGGGTGAT CAGAAGTGCT 
    GCTCCACCCC AAGAGAAAAA AAGGAAAGTG GAAAAGGATG CCATGACTGA GCACGCCCTG 
    GAGGCCCTGT CAGCCTCCCT GGGCACCCGG AAGCCGGAGC CGGAGCTCGA CCCCAGCTCC 
    ATTAAGGAGG TCGATGAGGC AAAAGCCAAA GAAGAGAAAG TAAAGAAATG TGGTGAAGAT 
    GAGGAAACAG TCCCATCGGA GTACAGATTA AAACCGGCCA CAGATAAAGA TGGAAAACCA 
    CTCTTGCCAG AGGCTGAAGA AAAACCCAAG CCCCTGAGTG AATCAGAACT CATCGATGAA 
    CTCTCAGAAG ATTTTGACCA GTCTAAGTGT AAAGAAAAAC AATCTAAGCC AACTGAAAAA 
    ACAGAGGCAT CCCCGGCCGC TGCCCCTGTA CCCGTGGCAG AGGACGTGCC TCGGACCTCT 
    ATGTGTTCCG TGCAGTCGGC TCCGCCCACA GCAGCTCCAG TGAAGGGCAT GGTGCCAGAC 
    GATGCTGTTG AAGCCTTGGC TGGAAGCCTG GGCAAAAAGG AAGCAGATCC AGAAGACGGA 
    AAGCCTGTGG AGGATAAAGT CAAGGAAAAA GCCAAAGAAG AGGATCGTGA GAAACTTGGT 
    GAAAAAGAAG AAACGATTCC TCCTGATTAC AGATTAGAAG AAGCCAAGGA TAAAGACGGA 
    AAACCACTGC TGCCAAAAGA GGTCAAGGAA CCGCTCCCAC CCTTGAGTGA AGACGTCCTC 
    CTCGATGCTC TGTCCAAGGA CTTCACTGTC CCCTCAGACA CATCATCGCC TCAATTTGAA 
    GATGCTAAAC TTTCAGCTGT CGTCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACC 
    CAGGCAGCCG GTCCACCCCC CAGCACTGCG CAGCGTGACA ACAAAGAACT TGACGATGCC 
    CTGGATCAAC TTTCTGACAG TCTCGGGCAA AGACAGCCTG ATCCAGATGA GAATAAACCC 
    GTAGAGGATA AAGTCAAGGA AAAAGCCAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
    GATGACACCA TCCCACCTAA ATACCAACAT CTTTTGGATG ACAACAAGGA GGGCACACCC 
    GGGAAGCCAA AGGCATCAGA GAAGCCCAAG GCATCAGAGA AACCTGCAGG TGCCCAGGAC 
    CCCATTGATG CCCTCTCAGG GGACTTTGAC AGCTGTCCCT CGACTACAGA AACCTCGACA 
    GACACACCAA AGGACAAAGA CAAGAAGCCT GCTTCCAGTG CCGAAGCACC TAGGAATGGC 
    GGGAAAGCAA AGGATTCCAC AAAGGCAAAG GAGGAAACTT CCAAGCCAAA AGCTGATGGA 
    AAAAGTACAA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209808.1
    CDS134..2428
    Translation

    Target ORF information:

    RefSeq Version XM_005209751.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209751.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGATGAAAAA GCCCTGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTGT AAAAACAGAA 
    CCTGAGAAGA AGCCACAATC ATCTAAGCCA TCTGTGGTTC ATGAGAAAAA AACCCAAGAA 
    GTAAAGCCAA AGGAACACAC AGAGCCAAAA AGCCTACCCA AGCACTCATC AGATACAGGA 
    AGCAAGCATG CTCCTAAGGA AAAAGCCGTT TCCAAATCAA GTGAGCAGCC ACCATCAGAG 
    AAATCAACAA AACCAAAGAC CAAGTCACAG GACAAGATCT CCGGTGGTGG AAAGAGCACT 
    GTTCCTGCTG CTGCTGCTGC AGCATCTGCC GAACCAGCTG ACAAGAATAA AGAAAATAAA 
    TTGTTAACAT CGGCCGTACC AGCTGAATCT AAACCAAGTA AACCATCTGG AAAGTCAGAC 
    ATGGACACTG CTCTGGATGA CTTAATAGAC ACTTTAGGAG AACCTGAAGA GATGAAAGAA 
    GATAACACAA CATATACCGG ACCGGAAGTG TCGGATCCAA TGAGTTCTAC CTACATAGAG 
    GAACTGGGTA AAAGAGAATC CACACTTCCT CCAAAATATA AGGAACTTCT GAATAAAGAA 
    GAAGGGATCG CAGGGCCTCC TCCAGACTCC TTGAAACCCC TGGGGCCCAA TGATGCCATC 
    GATGCCTTGT CATCCGACTT CACCTGCAGT TCCCCTACAG CTGATGCAAA GAAAACTGAG 
    AAAGAGAAAT CTACAGAAGA GGCTTTAAAA GCTCAGTCAG CTGGGGTGAT CAGAAGTGCT 
    GCTCCACCCC AAGAGAAAAA AAGGAAAGTG GAAAAGGATG CCATGACTGA GCACGCCCTG 
    GAGGCCCTGT CAGCCTCCCT GGGCACCCGG AAGCCGGAGC CGGAGCTCGA CCCCAGCTCC 
    ATTAAGGAGG TCGATGAGGC AAAAGCCAAA GAAGAGAAAG TAAAGAAATG TGGTGAAGAT 
    GAGGAAACAG TCCCATCGGA GTACAGATTA AAACCGGCCA CAGATAAAGA TGGAAAACCA 
    CTCTTGCCAG AGGCTGAAGA AAAACCCAAG CCCCTGAGTG AATCAGAACT CATCGATGAA 
    CTCTCAGAAG ATTTTGACCA GTCTAAGTGT AAAGAAAAAC AATCTAAGCC AACTGAAAAA 
    ACAGAGGCAT CCCCGGCCGC TGCCCCTGTA CCCGTGGCAG AGGACGTGCC TCGGACCTCT 
    ATGTGTTCCG TGCAGTCGGC TCCGCCCACA GCAGCTCCAG TGAAGGGCAT GGTGCCAGAC 
    GATGCTGTTG AAGCCTTGGC TGGAAGCCTG GGCAAAAAGG AAGCAGATCC AGAAGACGGA 
    AAGCCTGTGG AGGATAAAGT CAAGGAAAAA GCCAAAGAAG AGGATCGTGA GAAACTTGGT 
    GAAAAAGAAG AAACGATTCC TCCTGATTAC AGATTAGAAG AAGCCAAGGA TAAAGACGGA 
    AAACCACTGC TGCCAAAAGA GGTCAAGGAA CCGCTCCCAC CCTTGAGTGA AGACGTCCTC 
    CTCGATGCTC TGTCCAAGGA CTTCACTGTC CCCTCAGACA CATCATCGCC TCAATTTGAA 
    GATGCTAAAC TTTCAGCTGT CGTCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACC 
    CAGGCAGCCG GTCCACCCCC CAGCACTGCG CAGCGTGACA ACAAAGAACT TGACGATGCC 
    CTGGATCAAC TTTCTGACAG TCTCGGGCAA AGACAGCCTG ATCCAGATGA GAATAAACCC 
    GTAGAGGATA AAGTCAAGGA AAAAGCCAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
    GATGACACCA TCCCACCTAA ATACCAACAT CTTTTGGATG ACAACAAGGA GGGCACACCC 
    GGGAAGCCAA AGGCATCAGA GAAGCCCAAG GCATCAGAGA AACCTGCAGG TGCCCAGGAC 
    CCCATTGATG CCCTCTCAGG GGACTTTGAC AGCTGTCCCT CGACTACAGA AACCTCGACA 
    GACACACCAA AGGACAAAGA CAAGAAGCCT GCTTCCAGTG CCGAAGCACC TAGGAATGGC 
    GGGAAAGCAA AGGATTCCAC AAAGGCAAAG GAGGAAACTT CCAAGCCAAA AGCTGATGGA 
    AAAAGTACAA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209754.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209811.1 Bos taurus calpastatin (CAST), transcript variant X16, mRNA.
    Price & Availability↑
    CloneID OBa21780
    Clone ID Related Accession (Same CDS sequence) XM_005209754.3
    Accession Version XM_005209754.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGATGAAAAA GCCCTGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTAT TCCAGGCAGC 
    AAACAGCTGG AAGGACCGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGTAAAA 
    ACAGAACCTG AGAAGAAGCC ACAATCATCT AAGCCATCTG TGGTTCATGA GAAAAAAACC 
    CAAGAAGTAA AGCCAAAGGA ACACACAGAG CCAAAAAGCC TACCCAAGCA CTCATCAGAT 
    ACAGGAAGCA AGCATGCTCC TAAGGAAAAA GCCGTTTCCA AATCAAGTGA GCAGCCACCA 
    TCAGAGAAAT CAACAAAACC AAAGACCAAG TCACAGGACA AGATCTCCGG TGGTGGAAAG 
    AGCACTGTTC CTGCTGCTGC TGCTGCAGCA TCTGCCGAAC CAGCTGACAA GTCAGACATG 
    GACACTGCTC TGGATGACTT AATAGACACT TTAGGAGAAC CTGAAGAGAT GAAAGAAGAT 
    AACACAACAT ATACCGGACC GGAAGTGTCG GATCCAATGA GTTCTACCTA CATAGAGGAA 
    CTGGGTAAAA GAGAATCCAC ACTTCCTCCA AAATATAAGG AACTTCTGAA TAAAGAAGAA 
    GGGATCGCAG GGCCTCCTCC AGACTCCTTG AAACCCCTGG GGCCCAATGA TGCCATCGAT 
    GCCTTGTCAT CCGACTTCAC CTGCAGTTCC CCTACAGCTG ATGCAAAGAA AACTGAGAAA 
    GAGAAATCTA CAGAAGAGGC TTTAAAAGCT CAGTCAGCTG GGGTGATCAG AAGTGCTGCT 
    CCACCCCAAG AGAAAAAAAG GAAAGTGGAA AAGGATGCCA TGACTGAGCA CGCCCTGGAG 
    GCCCTGTCAG CCTCCCTGGG CACCCGGAAG CCGGAGCCGG AGCTCGACCC CAGCTCCATT 
    AAGGAGGTCG ATGAGGCAAA AGCCAAAGAA GAGAAAGTAA AGAAATGTGG TGAAGATGAG 
    GAAACAGTCC CATCGGAGTA CAGATTAAAA CCGGCCACAG ATAAAGATGG AAAACCACTC 
    TTGCCAGAGG CTGAAGAAAA ACCCAAGCCC CTGAGTGAAT CAGAACTCAT CGATGAACTC 
    TCAGAAGATT TTGACCAGTC TAAGTGTAAA GAAAAACAAT CTAAGCCAAC TGAAAAAACA 
    GAGGCATCCC CGGCCGCTGC CCCTGTACCC GTGGCAGAGG ACGTGCCTCG GACCTCTATG 
    TGTTCCGTGC AGTCGGCTCC GCCCACAGCA GCTCCAGTGA AGGGCATGGT GCCAGACGAT 
    GCTGTTGAAG CCTTGGCTGG AAGCCTGGGC AAAAAGGAAG CAGATCCAGA AGACGGAAAG 
    CCTGTGGAGG ATAAAGTCAA GGAAAAAGCC AAAGAAGAGG ATCGTGAGAA ACTTGGTGAA 
    AAAGAAGAAA CGATTCCTCC TGATTACAGA TTAGAAGAAG CCAAGGATAA AGACGGAAAA 
    CCACTGCTGC CAAAAGAGGT CAAGGAACCG CTCCCACCCT TGAGTGAAGA CGTCCTCCTC 
    GATGCTCTGT CCAAGGACTT CACTGTCCCC TCAGACACAT CATCGCCTCA ATTTGAAGAT 
    GCTAAACTTT CAGCTGTCGT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACCCAG 
    GCAGCCGGTC CACCCCCCAG CACTGCGCAG CGTGACAACA AAGAACTTGA CGATGCCCTG 
    GATCAACTTT CTGACAGTCT CGGGCAAAGA CAGCCTGATC CAGATGAGAA TAAACCCGTA 
    GAGGATAAAG TCAAGGAAAA AGCCAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAT 
    GACACCATCC CACCTAAATA CCAACATCTT TTGGATGACA ACAAGGAGGG CACACCCGGG 
    AAGCCAAAGG CATCAGAGAA GCCCAAGGCA TCAGAGAAAC CTGCAGGTGC CCAGGACCCC 
    ATTGATGCCC TCTCAGGGGA CTTTGACAGC TGTCCCTCGA CTACAGAAAC CTCGACAGAC 
    ACACCAAAGG ACAAAGACAA GAAGCCTGCT TCCAGTGCCG AAGCACCTAG GAATGGCGGG 
    AAAGCAAAGG ATTCCACAAA GGCAAAGGAG GAAACTTCCA AGCCAAAAGC TGATGGAAAA 
    AGTACAAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209811.1
    CDS131..2422
    Translation

    Target ORF information:

    RefSeq Version XM_005209754.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209754.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGATGAAAAA GCCCTGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTAT TCCAGGCAGC 
    AAACAGCTGG AAGGACCGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGTAAAA 
    ACAGAACCTG AGAAGAAGCC ACAATCATCT AAGCCATCTG TGGTTCATGA GAAAAAAACC 
    CAAGAAGTAA AGCCAAAGGA ACACACAGAG CCAAAAAGCC TACCCAAGCA CTCATCAGAT 
    ACAGGAAGCA AGCATGCTCC TAAGGAAAAA GCCGTTTCCA AATCAAGTGA GCAGCCACCA 
    TCAGAGAAAT CAACAAAACC AAAGACCAAG TCACAGGACA AGATCTCCGG TGGTGGAAAG 
    AGCACTGTTC CTGCTGCTGC TGCTGCAGCA TCTGCCGAAC CAGCTGACAA GTCAGACATG 
    GACACTGCTC TGGATGACTT AATAGACACT TTAGGAGAAC CTGAAGAGAT GAAAGAAGAT 
    AACACAACAT ATACCGGACC GGAAGTGTCG GATCCAATGA GTTCTACCTA CATAGAGGAA 
    CTGGGTAAAA GAGAATCCAC ACTTCCTCCA AAATATAAGG AACTTCTGAA TAAAGAAGAA 
    GGGATCGCAG GGCCTCCTCC AGACTCCTTG AAACCCCTGG GGCCCAATGA TGCCATCGAT 
    GCCTTGTCAT CCGACTTCAC CTGCAGTTCC CCTACAGCTG ATGCAAAGAA AACTGAGAAA 
    GAGAAATCTA CAGAAGAGGC TTTAAAAGCT CAGTCAGCTG GGGTGATCAG AAGTGCTGCT 
    CCACCCCAAG AGAAAAAAAG GAAAGTGGAA AAGGATGCCA TGACTGAGCA CGCCCTGGAG 
    GCCCTGTCAG CCTCCCTGGG CACCCGGAAG CCGGAGCCGG AGCTCGACCC CAGCTCCATT 
    AAGGAGGTCG ATGAGGCAAA AGCCAAAGAA GAGAAAGTAA AGAAATGTGG TGAAGATGAG 
    GAAACAGTCC CATCGGAGTA CAGATTAAAA CCGGCCACAG ATAAAGATGG AAAACCACTC 
    TTGCCAGAGG CTGAAGAAAA ACCCAAGCCC CTGAGTGAAT CAGAACTCAT CGATGAACTC 
    TCAGAAGATT TTGACCAGTC TAAGTGTAAA GAAAAACAAT CTAAGCCAAC TGAAAAAACA 
    GAGGCATCCC CGGCCGCTGC CCCTGTACCC GTGGCAGAGG ACGTGCCTCG GACCTCTATG 
    TGTTCCGTGC AGTCGGCTCC GCCCACAGCA GCTCCAGTGA AGGGCATGGT GCCAGACGAT 
    GCTGTTGAAG CCTTGGCTGG AAGCCTGGGC AAAAAGGAAG CAGATCCAGA AGACGGAAAG 
    CCTGTGGAGG ATAAAGTCAA GGAAAAAGCC AAAGAAGAGG ATCGTGAGAA ACTTGGTGAA 
    AAAGAAGAAA CGATTCCTCC TGATTACAGA TTAGAAGAAG CCAAGGATAA AGACGGAAAA 
    CCACTGCTGC CAAAAGAGGT CAAGGAACCG CTCCCACCCT TGAGTGAAGA CGTCCTCCTC 
    GATGCTCTGT CCAAGGACTT CACTGTCCCC TCAGACACAT CATCGCCTCA ATTTGAAGAT 
    GCTAAACTTT CAGCTGTCGT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACCCAG 
    GCAGCCGGTC CACCCCCCAG CACTGCGCAG CGTGACAACA AAGAACTTGA CGATGCCCTG 
    GATCAACTTT CTGACAGTCT CGGGCAAAGA CAGCCTGATC CAGATGAGAA TAAACCCGTA 
    GAGGATAAAG TCAAGGAAAA AGCCAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAT 
    GACACCATCC CACCTAAATA CCAACATCTT TTGGATGACA ACAAGGAGGG CACACCCGGG 
    AAGCCAAAGG CATCAGAGAA GCCCAAGGCA TCAGAGAAAC CTGCAGGTGC CCAGGACCCC 
    ATTGATGCCC TCTCAGGGGA CTTTGACAGC TGTCCCTCGA CTACAGAAAC CTCGACAGAC 
    ACACCAAAGG ACAAAGACAA GAAGCCTGCT TCCAGTGCCG AAGCACCTAG GAATGGCGGG 
    AAAGCAAAGG ATTCCACAAA GGCAAAGGAG GAAACTTCCA AGCCAAAAGC TGATGGAAAA 
    AGTACAAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( NM_001030319.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001025490.2 Bos taurus calpastatin (CAST), transcript variant III, mRNA.
    Price & Availability↑
    CloneID OBa21778
    Clone ID Related Accession (Same CDS sequence) XM_024993826.1 , XM_010807619.1 , XM_005209752.2 , NM_001030319.4 , XM_005209752.1
    Accession Version NM_001030319.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Bos taurus(cattle)
    Product calpastatin isoform III
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On Jul 2, 2016 this sequence version replaced NM_001030319.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR5571262.10239.1, SRR5571262.18632.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001025490.2
    CDS1..2157
    Translation

    Target ORF information:

    RefSeq Version NM_001030319.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant III, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001030319.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_024993826.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849594.1 Bos taurus calpastatin (CAST), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OBa21778
    Clone ID Related Accession (Same CDS sequence) XM_024993826.1 , XM_010807619.1 , XM_005209752.2 , NM_001030319.4 , XM_005209752.1
    Accession Version XM_024993826.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849594.1
    CDS302..2458
    Translation

    Target ORF information:

    RefSeq Version XM_024993826.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024993826.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_010807619.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805921.1 Bos taurus calpastatin (CAST), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OBa21778
    Clone ID Related Accession (Same CDS sequence) XM_024993826.1 , XM_010807619.1 , XM_005209752.2 , NM_001030319.4 , XM_005209752.1
    Accession Version XM_010807619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product calpastatin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104062.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805921.1
    CDS45..2201
    Translation

    Target ORF information:

    RefSeq Version XM_010807619.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010807619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209752.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209809.1 Bos taurus calpastatin (CAST), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OBa21778
    Clone ID Related Accession (Same CDS sequence) XM_024993826.1 , XM_010807619.1 , XM_005209752.2 , NM_001030319.4 , XM_005209752.1
    Accession Version XM_005209752.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209809.1
    CDS169..2325
    Translation

    Target ORF information:

    RefSeq Version XM_005209752.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209752.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209752.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209809.1 Bos taurus calpastatin (CAST), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OBa21778
    Clone ID Related Accession (Same CDS sequence) XM_024993826.1 , XM_010807619.1 , XM_005209752.2 , NM_001030319.4 , XM_005209752.1
    Accession Version XM_005209752.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product calpastatin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104062.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209809.1
    CDS353..2509
    Translation

    Target ORF information:

    RefSeq Version XM_005209752.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCAGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209753.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209810.1 Bos taurus calpastatin (CAST), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OBa21779
    Clone ID Related Accession (Same CDS sequence) XM_005209753.1 , XM_024993828.1 , XM_005209753.2 , XM_010807620.2
    Accession Version XM_005209753.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product calpastatin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104062.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAGCCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGCC ACAATCATCT 
    AAGCCATCTG TGGTTCATGA GAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACACAGAG 
    CCAAAAAGCC TACCCAAGCA CTCATCAGAT ACAGGAAGCA AGCATGCTCC TAAGGAAAAA 
    GCCGTTTCCA AATCAAGTGA GCAGCCACCA TCAGAGAAAT CAACAAAACC AAAGACCAAG 
    TCACAGGACA AGATCTCCGG TGGTGGAAAG AGCACTGTTC CTGCTGCTGC TGCTGCAGCA 
    TCTGCCGAAC CAGCTGACAA GAATAAAGAA AATAAATTGT TAACATCGGC CGTACCAGCT 
    GAATCTAAAC CAAGTAAACC ATCTGGAAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209810.1
    CDS353..2443
    Translation

    Target ORF information:

    RefSeq Version XM_005209753.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAGCCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGCC ACAATCATCT 
    AAGCCATCTG TGGTTCATGA GAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACACAGAG 
    CCAAAAAGCC TACCCAAGCA CTCATCAGAT ACAGGAAGCA AGCATGCTCC TAAGGAAAAA 
    GCCGTTTCCA AATCAAGTGA GCAGCCACCA TCAGAGAAAT CAACAAAACC AAAGACCAAG 
    TCACAGGACA AGATCTCCGG TGGTGGAAAG AGCACTGTTC CTGCTGCTGC TGCTGCAGCA 
    TCTGCCGAAC CAGCTGACAA GAATAAAGAA AATAAATTGT TAACATCGGC CGTACCAGCT 
    GAATCTAAAC CAAGTAAACC ATCTGGAAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_024993828.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849596.1 Bos taurus calpastatin (CAST), transcript variant X13, mRNA.
    Price & Availability↑
    CloneID OBa21779
    Clone ID Related Accession (Same CDS sequence) XM_005209753.1 , XM_024993828.1 , XM_005209753.2 , XM_010807620.2
    Accession Version XM_024993828.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAGCCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGCC ACAATCATCT 
    AAGCCATCTG TGGTTCATGA GAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACACAGAG 
    CCAAAAAGCC TACCCAAGCA CTCATCAGAT ACAGGAAGCA AGCATGCTCC TAAGGAAAAA 
    GCCGTTTCCA AATCAAGTGA GCAGCCACCA TCAGAGAAAT CAACAAAACC AAAGACCAAG 
    TCACAGGACA AGATCTCCGG TGGTGGAAAG AGCACTGTTC CTGCTGCTGC TGCTGCAGCA 
    TCTGCCGAAC CAGCTGACAA GAATAAAGAA AATAAATTGT TAACATCGGC CGTACCAGCT 
    GAATCTAAAC CAAGTAAACC ATCTGGAAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849596.1
    CDS302..2392
    Translation

    Target ORF information:

    RefSeq Version XM_024993828.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024993828.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAGCCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGCC ACAATCATCT 
    AAGCCATCTG TGGTTCATGA GAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACACAGAG 
    CCAAAAAGCC TACCCAAGCA CTCATCAGAT ACAGGAAGCA AGCATGCTCC TAAGGAAAAA 
    GCCGTTTCCA AATCAAGTGA GCAGCCACCA TCAGAGAAAT CAACAAAACC AAAGACCAAG 
    TCACAGGACA AGATCTCCGG TGGTGGAAAG AGCACTGTTC CTGCTGCTGC TGCTGCAGCA 
    TCTGCCGAAC CAGCTGACAA GAATAAAGAA AATAAATTGT TAACATCGGC CGTACCAGCT 
    GAATCTAAAC CAAGTAAACC ATCTGGAAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209753.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209810.1 Bos taurus calpastatin (CAST), transcript variant X14, mRNA.
    Price & Availability↑
    CloneID OBa21779
    Clone ID Related Accession (Same CDS sequence) XM_005209753.1 , XM_024993828.1 , XM_005209753.2 , XM_010807620.2
    Accession Version XM_005209753.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAGCCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGCC ACAATCATCT 
    AAGCCATCTG TGGTTCATGA GAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACACAGAG 
    CCAAAAAGCC TACCCAAGCA CTCATCAGAT ACAGGAAGCA AGCATGCTCC TAAGGAAAAA 
    GCCGTTTCCA AATCAAGTGA GCAGCCACCA TCAGAGAAAT CAACAAAACC AAAGACCAAG 
    TCACAGGACA AGATCTCCGG TGGTGGAAAG AGCACTGTTC CTGCTGCTGC TGCTGCAGCA 
    TCTGCCGAAC CAGCTGACAA GAATAAAGAA AATAAATTGT TAACATCGGC CGTACCAGCT 
    GAATCTAAAC CAAGTAAACC ATCTGGAAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209810.1
    CDS202..2292
    Translation

    Target ORF information:

    RefSeq Version XM_005209753.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209753.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAGCCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGCC ACAATCATCT 
    AAGCCATCTG TGGTTCATGA GAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACACAGAG 
    CCAAAAAGCC TACCCAAGCA CTCATCAGAT ACAGGAAGCA AGCATGCTCC TAAGGAAAAA 
    GCCGTTTCCA AATCAAGTGA GCAGCCACCA TCAGAGAAAT CAACAAAACC AAAGACCAAG 
    TCACAGGACA AGATCTCCGG TGGTGGAAAG AGCACTGTTC CTGCTGCTGC TGCTGCAGCA 
    TCTGCCGAAC CAGCTGACAA GAATAAAGAA AATAAATTGT TAACATCGGC CGTACCAGCT 
    GAATCTAAAC CAAGTAAACC ATCTGGAAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_010807620.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805922.1 Bos taurus calpastatin (CAST), transcript variant X15, mRNA.
    Price & Availability↑
    CloneID OBa21779
    Clone ID Related Accession (Same CDS sequence) XM_005209753.1 , XM_024993828.1 , XM_005209753.2 , XM_010807620.2
    Accession Version XM_010807620.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807620.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAGCCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGCC ACAATCATCT 
    AAGCCATCTG TGGTTCATGA GAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACACAGAG 
    CCAAAAAGCC TACCCAAGCA CTCATCAGAT ACAGGAAGCA AGCATGCTCC TAAGGAAAAA 
    GCCGTTTCCA AATCAAGTGA GCAGCCACCA TCAGAGAAAT CAACAAAACC AAAGACCAAG 
    TCACAGGACA AGATCTCCGG TGGTGGAAAG AGCACTGTTC CTGCTGCTGC TGCTGCAGCA 
    TCTGCCGAAC CAGCTGACAA GAATAAAGAA AATAAATTGT TAACATCGGC CGTACCAGCT 
    GAATCTAAAC CAAGTAAACC ATCTGGAAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805922.1
    CDS45..2135
    Translation

    Target ORF information:

    RefSeq Version XM_010807620.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010807620.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAGCCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGCC ACAATCATCT 
    AAGCCATCTG TGGTTCATGA GAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACACAGAG 
    CCAAAAAGCC TACCCAAGCA CTCATCAGAT ACAGGAAGCA AGCATGCTCC TAAGGAAAAA 
    GCCGTTTCCA AATCAAGTGA GCAGCCACCA TCAGAGAAAT CAACAAAACC AAAGACCAAG 
    TCACAGGACA AGATCTCCGG TGGTGGAAAG AGCACTGTTC CTGCTGCTGC TGCTGCAGCA 
    TCTGCCGAAC CAGCTGACAA GAATAAAGAA AATAAATTGT TAACATCGGC CGTACCAGCT 
    GAATCTAAAC CAAGTAAACC ATCTGGAAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( NM_174003.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776428.1 Bos taurus calpastatin (CAST), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OBa41352
    Clone ID Related Accession (Same CDS sequence) NM_174003.2
    Accession Version NM_174003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2361bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Bos taurus(cattle)
    Product calpastatin isoform II
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF159246.1. On or before Mar 29, 2005 this sequence version replaced XM_611833.1, NM_174003.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF159246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGGTGAAAAA GCCCCGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTAT TCCAGGCAGC 
    AAACAGCTGG AAGGACCGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGTAAAA 
    ACAGAACCTG AGAAGAAGCC ACAATCATCT AAGCCATCTG TGGTTCATGA GAAAAAAACC 
    CAAGAAGTAA AGCCAAAGGA ACACACAGAG CCAAAAAGCC TACCCAAGCA CTCATCAGAT 
    ACAGGAAGCA AGCATGCTCC TAAGGAAAAA GCCGTTTCCA AATCAAGTGA GCAGCCACCA 
    TCAGAGAAAT CAACAATACC AAAGACCAAT TCCCAGGACA AGATCTCCGG TGGTGGAAAG 
    AGCACTGTTC CTGCTGCTGC TGCTGCAGCA TCTGCCGAAC CAGCTGACAA GAATAAAGAA 
    AATAAATTGT TAACATCGGC CGTACCAGCT GAATCTAAAC CAAGTAAACC ATCTGGAAAG 
    TCAGACATGG ACACTGCTCT GGATGACTTA ATAGACACTT TAGGAGAACC TGAAGAGATG 
    AAAGAAGATA ACACAACATA TACCGGACCG GAAGTGTCGG ATCCAATGAG TTCTACCTAC 
    ATAGAGGAAC TGGGTAAAAG AGAATCCACA CTTCCTCCAA AATATAAGGA ACTTCTGAAT 
    AAAGAAGAAG GGATCGCGGG GCCTCCTCCA GACTCCTTGA AACCCCTGGG GCCCAATGAT 
    GCCATCGATG CCTTGTCATC CGACTTCACC TGCAGTTCCC CTACAGCTGA TGCAAAGAAA 
    ACTGAGAAAG AGAAATCTAC AGAAGAGGCT TTAAAAGCTC AGTCAGCTGG GGTGATCAGA 
    AGTGCTGCTC CACCCCAAGA GAAAAAAAGG AAAGTGGAAA AGGATGCCAT GACTGAGCAC 
    GCCCTGGAGG CCCTGTCTGC CTCCCTGGGC ACCCGGAAGC CGGAGCCGGA GCTCGACCCC 
    AGCTCCATTA AGGAGGTCGA TGAGGCAAAA GCCAAAGAAG AGAAAGTAAA GAAATGTGGT 
    GAAGATGAGG AAACAGTCCC ATCGGAGTAC AGATTAAAAC CGGCCACAGA TAAAGATGGA 
    AAACCACTCT TGCCAGAGGC TGAAGAAAAA CCCAAGCCCC TGAGTGAATC AGAACTCATC 
    GATGAACTCT CAGAAGATTT TGACCAGTCT AAGTGTAAAG AAAAACAATC TAAGCCAACT 
    GAAAAAACAG AGGCATCCCC GGCCGCTGCC CCCGTGCCCG TGGCAGAGGA CGTGCCTCGG 
    ACCTCTATGT GTTCCGTGCA GTCGGCTCCG CCCACAGCAG CTCCAGCGAA GGGCATGGTG 
    CCAGACGATG CTGTCGAAGC CTTGGCTGGA AGCCTGGGCA AAAAGGAAGC AGATCCAGAA 
    GACGGAAAGC CTGTGGAGGA TAAAGTCAAG GAGAAAGCCA AAGAAGAGGA TCGTGAGAAA 
    CTTGGTGAAA AAGAAGAAAC GATTCCTCCT GATTACAGAT TAGAAGAAGC CAAGGATAAA 
    GACGGAAAAC CACTGCTGCC AAAAGAGGTC AAGGAACCGC TCCCACCCTT GAGTGAAGAC 
    GTCCTCCTCG ATGCTCTGTC CAAGGACTTC ACTGTCCCCT CAGACACATC ATCGCCTCAA 
    TTTGAAGATG CTAAACTTTC AGCTGTCGTC TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA 
    ACCACCCAGG CAGCCGGTCC ACCCCCCAGC ACTGCGCAGC GTGACAACAA AGAACTTGAC 
    GATGCCCTGG ATCAACTTTC TGACAGTCTC GGGCAAAGAC AGCCTGATCC AGATGAGAAT 
    AAACCCGTAG AGGATAAAGT CAAGGAAAAA GCCAAAGCTG AACACAGAGA CAAGCTGGGA 
    GAAAGAGATG ACACCATCCC ACCTAAATAC CAACATCTTT TGGATGACAA CAAGGAGGGC 
    ACACCCGGGA AGCCAAAGGC ATCAGAGAAG CCCAAGGCAT CAGAGAAACC TGCAGGTGCC 
    CAGGACCCCA TTGATGCCCT CTCAGGGGAC TTTGACAGCT GTCCCTCGAC TACAGAAACC 
    TCGACAGACA CACCAAAGGA CAAAGACAAG AAGCCTGCTT CCAGTGCCGA AGCACCTAGG 
    AATGGCGGGA AAGCAAAGGA TTCCACAAAG GCAAAGGAGG AAACTTCCAA GCCAAAAGCT 
    GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776428.1
    CDS129..2489
    Translation

    Target ORF information:

    RefSeq Version NM_174003.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_174003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGGTGAAAAA GCCCCGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTAT TCCAGGCAGC 
    AAACAGCTGG AAGGACCGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGTAAAA 
    ACAGAACCTG AGAAGAAGCC ACAATCATCT AAGCCATCTG TGGTTCATGA GAAAAAAACC 
    CAAGAAGTAA AGCCAAAGGA ACACACAGAG CCAAAAAGCC TACCCAAGCA CTCATCAGAT 
    ACAGGAAGCA AGCATGCTCC TAAGGAAAAA GCCGTTTCCA AATCAAGTGA GCAGCCACCA 
    TCAGAGAAAT CAACAATACC AAAGACCAAT TCCCAGGACA AGATCTCCGG TGGTGGAAAG 
    AGCACTGTTC CTGCTGCTGC TGCTGCAGCA TCTGCCGAAC CAGCTGACAA GAATAAAGAA 
    AATAAATTGT TAACATCGGC CGTACCAGCT GAATCTAAAC CAAGTAAACC ATCTGGAAAG 
    TCAGACATGG ACACTGCTCT GGATGACTTA ATAGACACTT TAGGAGAACC TGAAGAGATG 
    AAAGAAGATA ACACAACATA TACCGGACCG GAAGTGTCGG ATCCAATGAG TTCTACCTAC 
    ATAGAGGAAC TGGGTAAAAG AGAATCCACA CTTCCTCCAA AATATAAGGA ACTTCTGAAT 
    AAAGAAGAAG GGATCGCGGG GCCTCCTCCA GACTCCTTGA AACCCCTGGG GCCCAATGAT 
    GCCATCGATG CCTTGTCATC CGACTTCACC TGCAGTTCCC CTACAGCTGA TGCAAAGAAA 
    ACTGAGAAAG AGAAATCTAC AGAAGAGGCT TTAAAAGCTC AGTCAGCTGG GGTGATCAGA 
    AGTGCTGCTC CACCCCAAGA GAAAAAAAGG AAAGTGGAAA AGGATGCCAT GACTGAGCAC 
    GCCCTGGAGG CCCTGTCTGC CTCCCTGGGC ACCCGGAAGC CGGAGCCGGA GCTCGACCCC 
    AGCTCCATTA AGGAGGTCGA TGAGGCAAAA GCCAAAGAAG AGAAAGTAAA GAAATGTGGT 
    GAAGATGAGG AAACAGTCCC ATCGGAGTAC AGATTAAAAC CGGCCACAGA TAAAGATGGA 
    AAACCACTCT TGCCAGAGGC TGAAGAAAAA CCCAAGCCCC TGAGTGAATC AGAACTCATC 
    GATGAACTCT CAGAAGATTT TGACCAGTCT AAGTGTAAAG AAAAACAATC TAAGCCAACT 
    GAAAAAACAG AGGCATCCCC GGCCGCTGCC CCCGTGCCCG TGGCAGAGGA CGTGCCTCGG 
    ACCTCTATGT GTTCCGTGCA GTCGGCTCCG CCCACAGCAG CTCCAGCGAA GGGCATGGTG 
    CCAGACGATG CTGTCGAAGC CTTGGCTGGA AGCCTGGGCA AAAAGGAAGC AGATCCAGAA 
    GACGGAAAGC CTGTGGAGGA TAAAGTCAAG GAGAAAGCCA AAGAAGAGGA TCGTGAGAAA 
    CTTGGTGAAA AAGAAGAAAC GATTCCTCCT GATTACAGAT TAGAAGAAGC CAAGGATAAA 
    GACGGAAAAC CACTGCTGCC AAAAGAGGTC AAGGAACCGC TCCCACCCTT GAGTGAAGAC 
    GTCCTCCTCG ATGCTCTGTC CAAGGACTTC ACTGTCCCCT CAGACACATC ATCGCCTCAA 
    TTTGAAGATG CTAAACTTTC AGCTGTCGTC TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA 
    ACCACCCAGG CAGCCGGTCC ACCCCCCAGC ACTGCGCAGC GTGACAACAA AGAACTTGAC 
    GATGCCCTGG ATCAACTTTC TGACAGTCTC GGGCAAAGAC AGCCTGATCC AGATGAGAAT 
    AAACCCGTAG AGGATAAAGT CAAGGAAAAA GCCAAAGCTG AACACAGAGA CAAGCTGGGA 
    GAAAGAGATG ACACCATCCC ACCTAAATAC CAACATCTTT TGGATGACAA CAAGGAGGGC 
    ACACCCGGGA AGCCAAAGGC ATCAGAGAAG CCCAAGGCAT CAGAGAAACC TGCAGGTGCC 
    CAGGACCCCA TTGATGCCCT CTCAGGGGAC TTTGACAGCT GTCCCTCGAC TACAGAAACC 
    TCGACAGACA CACCAAAGGA CAAAGACAAG AAGCCTGCTT CCAGTGCCGA AGCACCTAGG 
    AATGGCGGGA AAGCAAAGGA TTCCACAAAG GCAAAGGAGG AAACTTCCAA GCCAAAAGCT 
    GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_024993825.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849593.1 Bos taurus calpastatin (CAST), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OBa235758
    Clone ID Related Accession (Same CDS sequence) XM_024993825.1
    Accession Version XM_024993825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGATGAAAAA GCCCTGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTAT TCCAGGCAGC 
    AAACAGCTGG AAGGACCGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGTAAAA 
    ACAGAACCTG AGAAGAAGCC ACAATCATCT AAGCCATCTG TGGTTCATGA GAAAAAAACC 
    CAAGAAGTAA AGCCAAAGGA ACACACAGAG CCAAAAAGCC TACCCAAGCA CTCATCAGAT 
    ACAGGAAGCA AGCATGCTCC TAAGGAAAAA GCCGTTTCCA AATCAAGTGA GCAGCCACCA 
    TCAGAGAAAT CAACAAAACC AAAGACCAAG TCACAGGACA AGATCTCCGG TGGTGGAAAG 
    AGCACTGTTC CTGCTGCTGC TGCTGCAGCA TCTGCCGAAC CAGCTGACAA GAATAAAGAA 
    AATAAATTGT TAACATCGGC CGTACCAGCT GAATCTAAAC CAAGTAAACC ATCTGGAAAG 
    TCAGACATGG ACACTGCTCT GGATGACTTA ATAGACACTT TAGGAGAACC TGAAGAGATG 
    AAAGAAGATA ACACAACATA TACCGGACCG GAAGTGTCGG ATCCAATGAG TTCTACCTAC 
    ATAGAGGAAC TGGGTAAAAG AGAATCCACA CTTCCTCCAA AATATAAGGA ACTTCTGAAT 
    AAAGAAGAAG GGATCGCAGG GCCTCCTCCA GACTCCTTGA AACCCCTGGG GCCCAATGAT 
    GCCATCGATG CCTTGTCATC CGACTTCACC TGCAGTTCCC CTACAGCTGA TGCAAAGAAA 
    ACTGAGAAAG AGAAATCTAC AGAAGAGGCT TTAAAAGCTC AGTCAGCTGG GGTGATCAGA 
    AGTGCTGCTC CACCCCAAGA GAAAAAAAGG AAAGTGGAAA AGGATGCCAT GACTGAGCAC 
    GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC ACCCGGAAGC CGGAGCCGGA GCTCGACCCC 
    AGCTCCATTA AGGAGGTCGA TGAGGCAAAA GCCAAAGAAG AGAAAGTAAA GAAATGTGGT 
    GAAGATGAGG AAACAGTCCC ATCGGAGTAC AGATTAAAAC CGGCCACAGA TAAAGATGGA 
    AAACCACTCT TGCCAGAGGC TGAAGAAAAA CCCAAGCCCC TGAGTGAATC AGAACTCATC 
    GATGAACTCT CAGAAGATTT TGACCAGTCT AAGTGTAAAG AAAAACAATC TAAGCCAACT 
    GAAAAAACAG AGGCATCCCC GGCCGCTGCC CCTGTACCCG TGGCAGAGGA CGTGCCTCGG 
    ACCTCTATGT GTTCCGTGCA GTCGGCTCCG CCCACAGCAG CTCCAGTGAA GGGCATGGTG 
    CCAGACGATG CTGTTGAAGC CTTGGCTGGA AGCCTGGGCA AAAAGGAAGC AGATCCAGAA 
    GACGGAAAGC CTGTGGAGGA TAAAGTCAAG GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA 
    CTTGGTGAAA AAGAAGAAAC GATTCCTCCT GATTACAGAT TAGAAGAAGC CAAGGATAAA 
    GACGGAAAAC CACTGCTGCC AAAAGAGGTC AAGGAACCGC TCCCACCCTT GAGTGAAGAC 
    GTCCTCCTCG ATGCTCTGTC CAAGGACTTC ACTGTCCCCT CAGACACATC ATCGCCTCAA 
    TTTGAAGATG CTAAACTTTC AGCTGTCGTC TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA 
    ACCACCCAGG CAGCCGGTCC ACCCCCCAGC ACTGCGCGTG ACAACAAAGA ACTTGACGAT 
    GCCCTGGATC AACTTTCTGA CAGTCTCGGG CAAAGACAGC CTGATCCAGA TGAGAATAAA 
    CCCGTAGAGG ATAAAGTCAA GGAAAAAGCC AAAGCTGAAC ACAGAGACAA GCTGGGAGAA 
    AGAGATGACA CCATCCCACC TAAATACCAA CATCTTTTGG ATGACAACAA GGAGGGCACA 
    CCCGGGAAGC CAAAGGCATC AGAGAAGCCC AAGGCATCAG AGAAACCTGC AGGTGCCCAG 
    GACCCCATTG ATGCCCTCTC AGGGGACTTT GACAGCTGTC CCTCGACTAC AGAAACCTCG 
    ACAGACACAC CAAAGGACAA AGACAAGAAG CCTGCTTCCA GTGCCGAAGC ACCTAGGAAT 
    GGCGGGAAAG CAAAGGATTC CACAAAGGCA AAGGAGGAAA CTTCCAAGCC AAAAGCTGAT 
    GGAAAAAGTA CAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849593.1
    CDS130..2487
    Translation

    Target ORF information:

    RefSeq Version XM_024993825.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024993825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCATTTG CAAGCTGGTG GTACAAGACG CATGTCAGTA GAAAAACCAG TGGATCGCCT 
    TCCAAGTCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAACAAGCCT TGGGAGCAGT 
    CAGCTCTCCA GAACTCAGGC TGATGAAAAA GCCCTGGTCC CCAAGGTAAC TACTTCCTCT 
    GCGTCAGCCA GCAAGTCTTC CAGTATGAAT CCCACAGAAG CCAAGGCTAT TCCAGGCAGC 
    AAACAGCTGG AAGGACCGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGTAAAA 
    ACAGAACCTG AGAAGAAGCC ACAATCATCT AAGCCATCTG TGGTTCATGA GAAAAAAACC 
    CAAGAAGTAA AGCCAAAGGA ACACACAGAG CCAAAAAGCC TACCCAAGCA CTCATCAGAT 
    ACAGGAAGCA AGCATGCTCC TAAGGAAAAA GCCGTTTCCA AATCAAGTGA GCAGCCACCA 
    TCAGAGAAAT CAACAAAACC AAAGACCAAG TCACAGGACA AGATCTCCGG TGGTGGAAAG 
    AGCACTGTTC CTGCTGCTGC TGCTGCAGCA TCTGCCGAAC CAGCTGACAA GAATAAAGAA 
    AATAAATTGT TAACATCGGC CGTACCAGCT GAATCTAAAC CAAGTAAACC ATCTGGAAAG 
    TCAGACATGG ACACTGCTCT GGATGACTTA ATAGACACTT TAGGAGAACC TGAAGAGATG 
    AAAGAAGATA ACACAACATA TACCGGACCG GAAGTGTCGG ATCCAATGAG TTCTACCTAC 
    ATAGAGGAAC TGGGTAAAAG AGAATCCACA CTTCCTCCAA AATATAAGGA ACTTCTGAAT 
    AAAGAAGAAG GGATCGCAGG GCCTCCTCCA GACTCCTTGA AACCCCTGGG GCCCAATGAT 
    GCCATCGATG CCTTGTCATC CGACTTCACC TGCAGTTCCC CTACAGCTGA TGCAAAGAAA 
    ACTGAGAAAG AGAAATCTAC AGAAGAGGCT TTAAAAGCTC AGTCAGCTGG GGTGATCAGA 
    AGTGCTGCTC CACCCCAAGA GAAAAAAAGG AAAGTGGAAA AGGATGCCAT GACTGAGCAC 
    GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC ACCCGGAAGC CGGAGCCGGA GCTCGACCCC 
    AGCTCCATTA AGGAGGTCGA TGAGGCAAAA GCCAAAGAAG AGAAAGTAAA GAAATGTGGT 
    GAAGATGAGG AAACAGTCCC ATCGGAGTAC AGATTAAAAC CGGCCACAGA TAAAGATGGA 
    AAACCACTCT TGCCAGAGGC TGAAGAAAAA CCCAAGCCCC TGAGTGAATC AGAACTCATC 
    GATGAACTCT CAGAAGATTT TGACCAGTCT AAGTGTAAAG AAAAACAATC TAAGCCAACT 
    GAAAAAACAG AGGCATCCCC GGCCGCTGCC CCTGTACCCG TGGCAGAGGA CGTGCCTCGG 
    ACCTCTATGT GTTCCGTGCA GTCGGCTCCG CCCACAGCAG CTCCAGTGAA GGGCATGGTG 
    CCAGACGATG CTGTTGAAGC CTTGGCTGGA AGCCTGGGCA AAAAGGAAGC AGATCCAGAA 
    GACGGAAAGC CTGTGGAGGA TAAAGTCAAG GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA 
    CTTGGTGAAA AAGAAGAAAC GATTCCTCCT GATTACAGAT TAGAAGAAGC CAAGGATAAA 
    GACGGAAAAC CACTGCTGCC AAAAGAGGTC AAGGAACCGC TCCCACCCTT GAGTGAAGAC 
    GTCCTCCTCG ATGCTCTGTC CAAGGACTTC ACTGTCCCCT CAGACACATC ATCGCCTCAA 
    TTTGAAGATG CTAAACTTTC AGCTGTCGTC TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA 
    ACCACCCAGG CAGCCGGTCC ACCCCCCAGC ACTGCGCGTG ACAACAAAGA ACTTGACGAT 
    GCCCTGGATC AACTTTCTGA CAGTCTCGGG CAAAGACAGC CTGATCCAGA TGAGAATAAA 
    CCCGTAGAGG ATAAAGTCAA GGAAAAAGCC AAAGCTGAAC ACAGAGACAA GCTGGGAGAA 
    AGAGATGACA CCATCCCACC TAAATACCAA CATCTTTTGG ATGACAACAA GGAGGGCACA 
    CCCGGGAAGC CAAAGGCATC AGAGAAGCCC AAGGCATCAG AGAAACCTGC AGGTGCCCAG 
    GACCCCATTG ATGCCCTCTC AGGGGACTTT GACAGCTGTC CCTCGACTAC AGAAACCTCG 
    ACAGACACAC CAAAGGACAA AGACAAGAAG CCTGCTTCCA GTGCCGAAGC ACCTAGGAAT 
    GGCGGGAAAG CAAAGGATTC CACAAAGGCA AAGGAGGAAA CTTCCAAGCC AAAAGCTGAT 
    GGAAAAAGTA CAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_024993827.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849595.1 Bos taurus calpastatin (CAST), transcript variant X12, mRNA.
    Price & Availability↑
    CloneID OBa235759
    Clone ID Related Accession (Same CDS sequence) XM_024993827.1
    Accession Version XM_024993827.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCGTGACAA CAAAGAACTT GACGATGCCC TGGATCAACT TTCTGACAGT 
    CTCGGGCAAA GACAGCCTGA TCCAGATGAG AATAAACCCG TAGAGGATAA AGTCAAGGAA 
    AAAGCCAAAG CTGAACACAG AGACAAGCTG GGAGAAAGAG ATGACACCAT CCCACCTAAA 
    TACCAACATC TTTTGGATGA CAACAAGGAG GGCACACCCG GGAAGCCAAA GGCATCAGAG 
    AAGCCCAAGG CATCAGAGAA ACCTGCAGGT GCCCAGGACC CCATTGATGC CCTCTCAGGG 
    GACTTTGACA GCTGTCCCTC GACTACAGAA ACCTCGACAG ACACACCAAA GGACAAAGAC 
    AAGAAGCCTG CTTCCAGTGC CGAAGCACCT AGGAATGGCG GGAAAGCAAA GGATTCCACA 
    AAGGCAAAGG AGGAAACTTC CAAGCCAAAA GCTGATGGAA AAAGTACAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849595.1
    CDS302..2455
    Translation

    Target ORF information:

    RefSeq Version XM_024993827.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024993827.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAGCCAA GGCTATTCCA GGCAGCAAAC AGCTGGAAGG ACCGCATTCT 
    CCTAACAAGA AAAGACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGCCACAA 
    TCATCTAAGC CATCTGTGGT TCATGAGAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
    ACAGAGCCAA AAAGCCTACC CAAGCACTCA TCAGATACAG GAAGCAAGCA TGCTCCTAAG 
    GAAAAAGCCG TTTCCAAATC AAGTGAGCAG CCACCATCAG AGAAATCAAC AAAACCAAAG 
    ACCAAGTCAC AGGACAAGAT CTCCGGTGGT GGAAAGAGCA CTGTTCCTGC TGCTGCTGCT 
    GCAGCATCTG CCGAACCAGC TGACAAGAAT AAAGAAAATA AATTGTTAAC ATCGGCCGTA 
    CCAGCTGAAT CTAAACCAAG TAAACCATCT GGAAAGTCAG ACATGGACAC TGCTCTGGAT 
    GACTTAATAG ACACTTTAGG AGAACCTGAA GAGATGAAAG AAGATAACAC AACATATACC 
    GGACCGGAAG TGTCGGATCC AATGAGTTCT ACCTACATAG AGGAACTGGG TAAAAGAGAA 
    TCCACACTTC CTCCAAAATA TAAGGAACTT CTGAATAAAG AAGAAGGGAT CGCAGGGCCT 
    CCTCCAGACT CCTTGAAACC CCTGGGGCCC AATGATGCCA TCGATGCCTT GTCATCCGAC 
    TTCACCTGCA GTTCCCCTAC AGCTGATGCA AAGAAAACTG AGAAAGAGAA ATCTACAGAA 
    GAGGCTTTAA AAGCTCAGTC AGCTGGGGTG ATCAGAAGTG CTGCTCCACC CCAAGAGAAA 
    AAAAGGAAAG TGGAAAAGGA TGCCATGACT GAGCACGCCC TGGAGGCCCT GTCAGCCTCC 
    CTGGGCACCC GGAAGCCGGA GCCGGAGCTC GACCCCAGCT CCATTAAGGA GGTCGATGAG 
    GCAAAAGCCA AAGAAGAGAA AGTAAAGAAA TGTGGTGAAG ATGAGGAAAC AGTCCCATCG 
    GAGTACAGAT TAAAACCGGC CACAGATAAA GATGGAAAAC CACTCTTGCC AGAGGCTGAA 
    GAAAAACCCA AGCCCCTGAG TGAATCAGAA CTCATCGATG AACTCTCAGA AGATTTTGAC 
    CAGTCTAAGT GTAAAGAAAA ACAATCTAAG CCAACTGAAA AAACAGAGGC ATCCCCGGCC 
    GCTGCCCCTG TACCCGTGGC AGAGGACGTG CCTCGGACCT CTATGTGTTC CGTGCAGTCG 
    GCTCCGCCCA CAGCAGCTCC AGTGAAGGGC ATGGTGCCAG ACGATGCTGT TGAAGCCTTG 
    GCTGGAAGCC TGGGCAAAAA GGAAGCAGAT CCAGAAGACG GAAAGCCTGT GGAGGATAAA 
    GTCAAGGAAA AAGCCAAAGA AGAGGATCGT GAGAAACTTG GTGAAAAAGA AGAAACGATT 
    CCTCCTGATT ACAGATTAGA AGAAGCCAAG GATAAAGACG GAAAACCACT GCTGCCAAAA 
    GAGGTCAAGG AACCGCTCCC ACCCTTGAGT GAAGACGTCC TCCTCGATGC TCTGTCCAAG 
    GACTTCACTG TCCCCTCAGA CACATCATCG CCTCAATTTG AAGATGCTAA ACTTTCAGCT 
    GTCGTCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CCCAGGCAGC CGGTCCACCC 
    CCCAGCACTG CGCGTGACAA CAAAGAACTT GACGATGCCC TGGATCAACT TTCTGACAGT 
    CTCGGGCAAA GACAGCCTGA TCCAGATGAG AATAAACCCG TAGAGGATAA AGTCAAGGAA 
    AAAGCCAAAG CTGAACACAG AGACAAGCTG GGAGAAAGAG ATGACACCAT CCCACCTAAA 
    TACCAACATC TTTTGGATGA CAACAAGGAG GGCACACCCG GGAAGCCAAA GGCATCAGAG 
    AAGCCCAAGG CATCAGAGAA ACCTGCAGGT GCCCAGGACC CCATTGATGC CCTCTCAGGG 
    GACTTTGACA GCTGTCCCTC GACTACAGAA ACCTCGACAG ACACACCAAA GGACAAAGAC 
    AAGAAGCCTG CTTCCAGTGC CGAAGCACCT AGGAATGGCG GGAAAGCAAA GGATTCCACA 
    AAGGCAAAGG AGGAAACTTC CAAGCCAAAA GCTGATGGAA AAAGTACAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( NM_001030318.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001025489.2 Bos taurus calpastatin (CAST), transcript variant I, mRNA.
    Price & Availability↑
    CloneID OBa21775
    Clone ID Related Accession (Same CDS sequence) XM_005209749.2 , XM_005209749.3 , XM_024993823.1 , XM_024993824.1 , NM_001030318.3
    Accession Version NM_001030318.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Bos taurus(cattle)
    Product calpastatin isoform I
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On Jul 2, 2016 this sequence version replaced NM_001030318.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR5571262.30862.1, SRR5765709.1262.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001025489.2
    CDS159..2558
    Translation

    Target ORF information:

    RefSeq Version NM_001030318.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001030318.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209749.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209806.1 Bos taurus calpastatin (CAST), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa21775
    Clone ID Related Accession (Same CDS sequence) XM_005209749.2 , XM_005209749.3 , XM_024993823.1 , XM_024993824.1 , NM_001030318.3
    Accession Version XM_005209749.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104062.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005209749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209806.1
    CDS154..2553
    Translation

    Target ORF information:

    RefSeq Version XM_005209749.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209749.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209749.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209806.1 Bos taurus calpastatin (CAST), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa21775
    Clone ID Related Accession (Same CDS sequence) XM_005209749.2 , XM_005209749.3 , XM_024993823.1 , XM_024993824.1 , NM_001030318.3
    Accession Version XM_005209749.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209806.1
    CDS493..2892
    Translation

    Target ORF information:

    RefSeq Version XM_005209749.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209749.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_024993823.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849591.1 Bos taurus calpastatin (CAST), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBa21775
    Clone ID Related Accession (Same CDS sequence) XM_005209749.2 , XM_005209749.3 , XM_024993823.1 , XM_024993824.1 , NM_001030318.3
    Accession Version XM_024993823.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849591.1
    CDS154..2553
    Translation

    Target ORF information:

    RefSeq Version XM_024993823.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024993823.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_024993824.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849592.1 Bos taurus calpastatin (CAST), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBa21775
    Clone ID Related Accession (Same CDS sequence) XM_005209749.2 , XM_005209749.3 , XM_024993823.1 , XM_024993824.1 , NM_001030318.3
    Accession Version XM_024993824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849592.1
    CDS154..2553
    Translation

    Target ORF information:

    RefSeq Version XM_024993824.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024993824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCAGCG TGACAACAAA GAACTTGACG ATGCCCTGGA TCAACTTTCT 
    GACAGTCTCG GGCAAAGACA GCCTGATCCA GATGAGAATA AACCCGTAGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGCTGA ACACAGAGAC AAGCTGGGAG AAAGAGATGA CACCATCCCA 
    CCTAAATACC AACATCTTTT GGATGACAAC AAGGAGGGCA CACCCGGGAA GCCAAAGGCA 
    TCAGAGAAGC CCAAGGCATC AGAGAAACCT GCAGGTGCCC AGGACCCCAT TGATGCCCTC 
    TCAGGGGACT TTGACAGCTG TCCCTCGACT ACAGAAACCT CGACAGACAC ACCAAAGGAC 
    AAAGACAAGA AGCCTGCTTC CAGTGCCGAA GCACCTAGGA ATGGCGGGAA AGCAAAGGAT 
    TCCACAAAGG CAAAGGAGGA AACTTCCAAG CCAAAAGCTG ATGGAAAAAG TACAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209750.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209807.1 Bos taurus calpastatin (CAST), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OBa21776
    Clone ID Related Accession (Same CDS sequence) XM_005209750.3 , XM_005209750.2
    Accession Version XM_005209750.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209750.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTGTA AAAACAGAAC CTGAGAAGAA GCCACAATCA 
    TCTAAGCCAT CTGTGGTTCA TGAGAAAAAA ACCCAAGAAG TAAAGCCAAA GGAACACACA 
    GAGCCAAAAA GCCTACCCAA GCACTCATCA GATACAGGAA GCAAGCATGC TCCTAAGGAA 
    AAAGCCGTTT CCAAATCAAG TGAGCAGCCA CCATCAGAGA AATCAACAAA ACCAAAGACC 
    AAGTCACAGG ACAAGATCTC CGGTGGTGGA AAGAGCACTG TTCCTGCTGC TGCTGCTGCA 
    GCATCTGCCG AACCAGCTGA CAAGAATAAA GAAAATAAAT TGTTAACATC GGCCGTACCA 
    GCTGAATCTA AACCAAGTAA ACCATCTGGA AAGTCAGACA TGGACACTGC TCTGGATGAC 
    TTAATAGACA CTTTAGGAGA ACCTGAAGAG ATGAAAGAAG ATAACACAAC ATATACCGGA 
    CCGGAAGTGT CGGATCCAAT GAGTTCTACC TACATAGAGG AACTGGGTAA AAGAGAATCC 
    ACACTTCCTC CAAAATATAA GGAACTTCTG AATAAAGAAG AAGGGATCGC AGGGCCTCCT 
    CCAGACTCCT TGAAACCCCT GGGGCCCAAT GATGCCATCG ATGCCTTGTC ATCCGACTTC 
    ACCTGCAGTT CCCCTACAGC TGATGCAAAG AAAACTGAGA AAGAGAAATC TACAGAAGAG 
    GCTTTAAAAG CTCAGTCAGC TGGGGTGATC AGAAGTGCTG CTCCACCCCA AGAGAAAAAA 
    AGGAAAGTGG AAAAGGATGC CATGACTGAG CACGCCCTGG AGGCCCTGTC AGCCTCCCTG 
    GGCACCCGGA AGCCGGAGCC GGAGCTCGAC CCCAGCTCCA TTAAGGAGGT CGATGAGGCA 
    AAAGCCAAAG AAGAGAAAGT AAAGAAATGT GGTGAAGATG AGGAAACAGT CCCATCGGAG 
    TACAGATTAA AACCGGCCAC AGATAAAGAT GGAAAACCAC TCTTGCCAGA GGCTGAAGAA 
    AAACCCAAGC CCCTGAGTGA ATCAGAACTC ATCGATGAAC TCTCAGAAGA TTTTGACCAG 
    TCTAAGTGTA AAGAAAAACA ATCTAAGCCA ACTGAAAAAA CAGAGGCATC CCCGGCCGCT 
    GCCCCTGTAC CCGTGGCAGA GGACGTGCCT CGGACCTCTA TGTGTTCCGT GCAGTCGGCT 
    CCGCCCACAG CAGCTCCAGT GAAGGGCATG GTGCCAGACG ATGCTGTTGA AGCCTTGGCT 
    GGAAGCCTGG GCAAAAAGGA AGCAGATCCA GAAGACGGAA AGCCTGTGGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGAAGA GGATCGTGAG AAACTTGGTG AAAAAGAAGA AACGATTCCT 
    CCTGATTACA GATTAGAAGA AGCCAAGGAT AAAGACGGAA AACCACTGCT GCCAAAAGAG 
    GTCAAGGAAC CGCTCCCACC CTTGAGTGAA GACGTCCTCC TCGATGCTCT GTCCAAGGAC 
    TTCACTGTCC CCTCAGACAC ATCATCGCCT CAATTTGAAG ATGCTAAACT TTCAGCTGTC 
    GTCTCTGAAG TGGTTTCCCA AACCCCAGCT CCAACCACCC AGGCAGCCGG TCCACCCCCC 
    AGCACTGCGC AGCGTGACAA CAAAGAACTT GACGATGCCC TGGATCAACT TTCTGACAGT 
    CTCGGGCAAA GACAGCCTGA TCCAGATGAG AATAAACCCG TAGAGGATAA AGTCAAGGAA 
    AAAGCCAAAG CTGAACACAG AGACAAGCTG GGAGAAAGAG ATGACACCAT CCCACCTAAA 
    TACCAACATC TTTTGGATGA CAACAAGGAG GGCACACCCG GGAAGCCAAA GGCATCAGAG 
    AAGCCCAAGG CATCAGAGAA ACCTGCAGGT GCCCAGGACC CCATTGATGC CCTCTCAGGG 
    GACTTTGACA GCTGTCCCTC GACTACAGAA ACCTCGACAG ACACACCAAA GGACAAAGAC 
    AAGAAGCCTG CTTCCAGTGC CGAAGCACCT AGGAATGGCG GGAAAGCAAA GGATTCCACA 
    AAGGCAAAGG AGGAAACTTC CAAGCCAAAA GCTGATGGAA AAAGTACAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209807.1
    CDS304..2637
    Translation

    Target ORF information:

    RefSeq Version XM_005209750.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209750.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTGTA AAAACAGAAC CTGAGAAGAA GCCACAATCA 
    TCTAAGCCAT CTGTGGTTCA TGAGAAAAAA ACCCAAGAAG TAAAGCCAAA GGAACACACA 
    GAGCCAAAAA GCCTACCCAA GCACTCATCA GATACAGGAA GCAAGCATGC TCCTAAGGAA 
    AAAGCCGTTT CCAAATCAAG TGAGCAGCCA CCATCAGAGA AATCAACAAA ACCAAAGACC 
    AAGTCACAGG ACAAGATCTC CGGTGGTGGA AAGAGCACTG TTCCTGCTGC TGCTGCTGCA 
    GCATCTGCCG AACCAGCTGA CAAGAATAAA GAAAATAAAT TGTTAACATC GGCCGTACCA 
    GCTGAATCTA AACCAAGTAA ACCATCTGGA AAGTCAGACA TGGACACTGC TCTGGATGAC 
    TTAATAGACA CTTTAGGAGA ACCTGAAGAG ATGAAAGAAG ATAACACAAC ATATACCGGA 
    CCGGAAGTGT CGGATCCAAT GAGTTCTACC TACATAGAGG AACTGGGTAA AAGAGAATCC 
    ACACTTCCTC CAAAATATAA GGAACTTCTG AATAAAGAAG AAGGGATCGC AGGGCCTCCT 
    CCAGACTCCT TGAAACCCCT GGGGCCCAAT GATGCCATCG ATGCCTTGTC ATCCGACTTC 
    ACCTGCAGTT CCCCTACAGC TGATGCAAAG AAAACTGAGA AAGAGAAATC TACAGAAGAG 
    GCTTTAAAAG CTCAGTCAGC TGGGGTGATC AGAAGTGCTG CTCCACCCCA AGAGAAAAAA 
    AGGAAAGTGG AAAAGGATGC CATGACTGAG CACGCCCTGG AGGCCCTGTC AGCCTCCCTG 
    GGCACCCGGA AGCCGGAGCC GGAGCTCGAC CCCAGCTCCA TTAAGGAGGT CGATGAGGCA 
    AAAGCCAAAG AAGAGAAAGT AAAGAAATGT GGTGAAGATG AGGAAACAGT CCCATCGGAG 
    TACAGATTAA AACCGGCCAC AGATAAAGAT GGAAAACCAC TCTTGCCAGA GGCTGAAGAA 
    AAACCCAAGC CCCTGAGTGA ATCAGAACTC ATCGATGAAC TCTCAGAAGA TTTTGACCAG 
    TCTAAGTGTA AAGAAAAACA ATCTAAGCCA ACTGAAAAAA CAGAGGCATC CCCGGCCGCT 
    GCCCCTGTAC CCGTGGCAGA GGACGTGCCT CGGACCTCTA TGTGTTCCGT GCAGTCGGCT 
    CCGCCCACAG CAGCTCCAGT GAAGGGCATG GTGCCAGACG ATGCTGTTGA AGCCTTGGCT 
    GGAAGCCTGG GCAAAAAGGA AGCAGATCCA GAAGACGGAA AGCCTGTGGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGAAGA GGATCGTGAG AAACTTGGTG AAAAAGAAGA AACGATTCCT 
    CCTGATTACA GATTAGAAGA AGCCAAGGAT AAAGACGGAA AACCACTGCT GCCAAAAGAG 
    GTCAAGGAAC CGCTCCCACC CTTGAGTGAA GACGTCCTCC TCGATGCTCT GTCCAAGGAC 
    TTCACTGTCC CCTCAGACAC ATCATCGCCT CAATTTGAAG ATGCTAAACT TTCAGCTGTC 
    GTCTCTGAAG TGGTTTCCCA AACCCCAGCT CCAACCACCC AGGCAGCCGG TCCACCCCCC 
    AGCACTGCGC AGCGTGACAA CAAAGAACTT GACGATGCCC TGGATCAACT TTCTGACAGT 
    CTCGGGCAAA GACAGCCTGA TCCAGATGAG AATAAACCCG TAGAGGATAA AGTCAAGGAA 
    AAAGCCAAAG CTGAACACAG AGACAAGCTG GGAGAAAGAG ATGACACCAT CCCACCTAAA 
    TACCAACATC TTTTGGATGA CAACAAGGAG GGCACACCCG GGAAGCCAAA GGCATCAGAG 
    AAGCCCAAGG CATCAGAGAA ACCTGCAGGT GCCCAGGACC CCATTGATGC CCTCTCAGGG 
    GACTTTGACA GCTGTCCCTC GACTACAGAA ACCTCGACAG ACACACCAAA GGACAAAGAC 
    AAGAAGCCTG CTTCCAGTGC CGAAGCACCT AGGAATGGCG GGAAAGCAAA GGATTCCACA 
    AAGGCAAAGG AGGAAACTTC CAAGCCAAAA GCTGATGGAA AAAGTACAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_005209750.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209807.1 Bos taurus calpastatin (CAST), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBa21776
    Clone ID Related Accession (Same CDS sequence) XM_005209750.3 , XM_005209750.2
    Accession Version XM_005209750.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product calpastatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104062.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005209750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTGTA AAAACAGAAC CTGAGAAGAA GCCACAATCA 
    TCTAAGCCAT CTGTGGTTCA TGAGAAAAAA ACCCAAGAAG TAAAGCCAAA GGAACACACA 
    GAGCCAAAAA GCCTACCCAA GCACTCATCA GATACAGGAA GCAAGCATGC TCCTAAGGAA 
    AAAGCCGTTT CCAAATCAAG TGAGCAGCCA CCATCAGAGA AATCAACAAA ACCAAAGACC 
    AAGTCACAGG ACAAGATCTC CGGTGGTGGA AAGAGCACTG TTCCTGCTGC TGCTGCTGCA 
    GCATCTGCCG AACCAGCTGA CAAGAATAAA GAAAATAAAT TGTTAACATC GGCCGTACCA 
    GCTGAATCTA AACCAAGTAA ACCATCTGGA AAGTCAGACA TGGACACTGC TCTGGATGAC 
    TTAATAGACA CTTTAGGAGA ACCTGAAGAG ATGAAAGAAG ATAACACAAC ATATACCGGA 
    CCGGAAGTGT CGGATCCAAT GAGTTCTACC TACATAGAGG AACTGGGTAA AAGAGAATCC 
    ACACTTCCTC CAAAATATAA GGAACTTCTG AATAAAGAAG AAGGGATCGC AGGGCCTCCT 
    CCAGACTCCT TGAAACCCCT GGGGCCCAAT GATGCCATCG ATGCCTTGTC ATCCGACTTC 
    ACCTGCAGTT CCCCTACAGC TGATGCAAAG AAAACTGAGA AAGAGAAATC TACAGAAGAG 
    GCTTTAAAAG CTCAGTCAGC TGGGGTGATC AGAAGTGCTG CTCCACCCCA AGAGAAAAAA 
    AGGAAAGTGG AAAAGGATGC CATGACTGAG CACGCCCTGG AGGCCCTGTC AGCCTCCCTG 
    GGCACCCGGA AGCCGGAGCC GGAGCTCGAC CCCAGCTCCA TTAAGGAGGT CGATGAGGCA 
    AAAGCCAAAG AAGAGAAAGT AAAGAAATGT GGTGAAGATG AGGAAACAGT CCCATCGGAG 
    TACAGATTAA AACCGGCCAC AGATAAAGAT GGAAAACCAC TCTTGCCAGA GGCTGAAGAA 
    AAACCCAAGC CCCTGAGTGA ATCAGAACTC ATCGATGAAC TCTCAGAAGA TTTTGACCAG 
    TCTAAGTGTA AAGAAAAACA ATCTAAGCCA ACTGAAAAAA CAGAGGCATC CCCGGCCGCT 
    GCCCCTGTAC CCGTGGCAGA GGACGTGCCT CGGACCTCTA TGTGTTCCGT GCAGTCGGCT 
    CCGCCCACAG CAGCTCCAGT GAAGGGCATG GTGCCAGACG ATGCTGTTGA AGCCTTGGCT 
    GGAAGCCTGG GCAAAAAGGA AGCAGATCCA GAAGACGGAA AGCCTGTGGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGAAGA GGATCGTGAG AAACTTGGTG AAAAAGAAGA AACGATTCCT 
    CCTGATTACA GATTAGAAGA AGCCAAGGAT AAAGACGGAA AACCACTGCT GCCAAAAGAG 
    GTCAAGGAAC CGCTCCCACC CTTGAGTGAA GACGTCCTCC TCGATGCTCT GTCCAAGGAC 
    TTCACTGTCC CCTCAGACAC ATCATCGCCT CAATTTGAAG ATGCTAAACT TTCAGCTGTC 
    GTCTCTGAAG TGGTTTCCCA AACCCCAGCT CCAACCACCC AGGCAGCCGG TCCACCCCCC 
    AGCACTGCGC AGCGTGACAA CAAAGAACTT GACGATGCCC TGGATCAACT TTCTGACAGT 
    CTCGGGCAAA GACAGCCTGA TCCAGATGAG AATAAACCCG TAGAGGATAA AGTCAAGGAA 
    AAAGCCAAAG CTGAACACAG AGACAAGCTG GGAGAAAGAG ATGACACCAT CCCACCTAAA 
    TACCAACATC TTTTGGATGA CAACAAGGAG GGCACACCCG GGAAGCCAAA GGCATCAGAG 
    AAGCCCAAGG CATCAGAGAA ACCTGCAGGT GCCCAGGACC CCATTGATGC CCTCTCAGGG 
    GACTTTGACA GCTGTCCCTC GACTACAGAA ACCTCGACAG ACACACCAAA GGACAAAGAC 
    AAGAAGCCTG CTTCCAGTGC CGAAGCACCT AGGAATGGCG GGAAAGCAAA GGATTCCACA 
    AAGGCAAAGG AGGAAACTTC CAAGCCAAAA GCTGATGGAA AAAGTACAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209807.1
    CDS154..2487
    Translation

    Target ORF information:

    RefSeq Version XM_005209750.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005209750.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTGTA AAAACAGAAC CTGAGAAGAA GCCACAATCA 
    TCTAAGCCAT CTGTGGTTCA TGAGAAAAAA ACCCAAGAAG TAAAGCCAAA GGAACACACA 
    GAGCCAAAAA GCCTACCCAA GCACTCATCA GATACAGGAA GCAAGCATGC TCCTAAGGAA 
    AAAGCCGTTT CCAAATCAAG TGAGCAGCCA CCATCAGAGA AATCAACAAA ACCAAAGACC 
    AAGTCACAGG ACAAGATCTC CGGTGGTGGA AAGAGCACTG TTCCTGCTGC TGCTGCTGCA 
    GCATCTGCCG AACCAGCTGA CAAGAATAAA GAAAATAAAT TGTTAACATC GGCCGTACCA 
    GCTGAATCTA AACCAAGTAA ACCATCTGGA AAGTCAGACA TGGACACTGC TCTGGATGAC 
    TTAATAGACA CTTTAGGAGA ACCTGAAGAG ATGAAAGAAG ATAACACAAC ATATACCGGA 
    CCGGAAGTGT CGGATCCAAT GAGTTCTACC TACATAGAGG AACTGGGTAA AAGAGAATCC 
    ACACTTCCTC CAAAATATAA GGAACTTCTG AATAAAGAAG AAGGGATCGC AGGGCCTCCT 
    CCAGACTCCT TGAAACCCCT GGGGCCCAAT GATGCCATCG ATGCCTTGTC ATCCGACTTC 
    ACCTGCAGTT CCCCTACAGC TGATGCAAAG AAAACTGAGA AAGAGAAATC TACAGAAGAG 
    GCTTTAAAAG CTCAGTCAGC TGGGGTGATC AGAAGTGCTG CTCCACCCCA AGAGAAAAAA 
    AGGAAAGTGG AAAAGGATGC CATGACTGAG CACGCCCTGG AGGCCCTGTC AGCCTCCCTG 
    GGCACCCGGA AGCCGGAGCC GGAGCTCGAC CCCAGCTCCA TTAAGGAGGT CGATGAGGCA 
    AAAGCCAAAG AAGAGAAAGT AAAGAAATGT GGTGAAGATG AGGAAACAGT CCCATCGGAG 
    TACAGATTAA AACCGGCCAC AGATAAAGAT GGAAAACCAC TCTTGCCAGA GGCTGAAGAA 
    AAACCCAAGC CCCTGAGTGA ATCAGAACTC ATCGATGAAC TCTCAGAAGA TTTTGACCAG 
    TCTAAGTGTA AAGAAAAACA ATCTAAGCCA ACTGAAAAAA CAGAGGCATC CCCGGCCGCT 
    GCCCCTGTAC CCGTGGCAGA GGACGTGCCT CGGACCTCTA TGTGTTCCGT GCAGTCGGCT 
    CCGCCCACAG CAGCTCCAGT GAAGGGCATG GTGCCAGACG ATGCTGTTGA AGCCTTGGCT 
    GGAAGCCTGG GCAAAAAGGA AGCAGATCCA GAAGACGGAA AGCCTGTGGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGAAGA GGATCGTGAG AAACTTGGTG AAAAAGAAGA AACGATTCCT 
    CCTGATTACA GATTAGAAGA AGCCAAGGAT AAAGACGGAA AACCACTGCT GCCAAAAGAG 
    GTCAAGGAAC CGCTCCCACC CTTGAGTGAA GACGTCCTCC TCGATGCTCT GTCCAAGGAC 
    TTCACTGTCC CCTCAGACAC ATCATCGCCT CAATTTGAAG ATGCTAAACT TTCAGCTGTC 
    GTCTCTGAAG TGGTTTCCCA AACCCCAGCT CCAACCACCC AGGCAGCCGG TCCACCCCCC 
    AGCACTGCGC AGCGTGACAA CAAAGAACTT GACGATGCCC TGGATCAACT TTCTGACAGT 
    CTCGGGCAAA GACAGCCTGA TCCAGATGAG AATAAACCCG TAGAGGATAA AGTCAAGGAA 
    AAAGCCAAAG CTGAACACAG AGACAAGCTG GGAGAAAGAG ATGACACCAT CCCACCTAAA 
    TACCAACATC TTTTGGATGA CAACAAGGAG GGCACACCCG GGAAGCCAAA GGCATCAGAG 
    AAGCCCAAGG CATCAGAGAA ACCTGCAGGT GCCCAGGACC CCATTGATGC CCTCTCAGGG 
    GACTTTGACA GCTGTCCCTC GACTACAGAA ACCTCGACAG ACACACCAAA GGACAAAGAC 
    AAGAAGCCTG CTTCCAGTGC CGAAGCACCT AGGAATGGCG GGAAAGCAAA GGATTCCACA 
    AAGGCAAAGG AGGAAACTTC CAAGCCAAAA GCTGATGGAA AAAGTACAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_010807616.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805918.1 Bos taurus calpastatin (CAST), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OBa45055
    Clone ID Related Accession (Same CDS sequence) XM_010807616.1
    Accession Version XM_010807616.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805918.1
    CDS154..2550
    Translation

    Target ORF information:

    RefSeq Version XM_010807616.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010807616.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG AATAAAGAAA ATAAATTGTT AACATCGGCC 
    GTACCAGCTG AATCTAAACC AAGTAAACCA TCTGGAAAGT CAGACATGGA CACTGCTCTG 
    GATGACTTAA TAGACACTTT AGGAGAACCT GAAGAGATGA AAGAAGATAA CACAACATAT 
    ACCGGACCGG AAGTGTCGGA TCCAATGAGT TCTACCTACA TAGAGGAACT GGGTAAAAGA 
    GAATCCACAC TTCCTCCAAA ATATAAGGAA CTTCTGAATA AAGAAGAAGG GATCGCAGGG 
    CCTCCTCCAG ACTCCTTGAA ACCCCTGGGG CCCAATGATG CCATCGATGC CTTGTCATCC 
    GACTTCACCT GCAGTTCCCC TACAGCTGAT GCAAAGAAAA CTGAGAAAGA GAAATCTACA 
    GAAGAGGCTT TAAAAGCTCA GTCAGCTGGG GTGATCAGAA GTGCTGCTCC ACCCCAAGAG 
    AAAAAAAGGA AAGTGGAAAA GGATGCCATG ACTGAGCACG CCCTGGAGGC CCTGTCAGCC 
    TCCCTGGGCA CCCGGAAGCC GGAGCCGGAG CTCGACCCCA GCTCCATTAA GGAGGTCGAT 
    GAGGCAAAAG CCAAAGAAGA GAAAGTAAAG AAATGTGGTG AAGATGAGGA AACAGTCCCA 
    TCGGAGTACA GATTAAAACC GGCCACAGAT AAAGATGGAA AACCACTCTT GCCAGAGGCT 
    GAAGAAAAAC CCAAGCCCCT GAGTGAATCA GAACTCATCG ATGAACTCTC AGAAGATTTT 
    GACCAGTCTA AGTGTAAAGA AAAACAATCT AAGCCAACTG AAAAAACAGA GGCATCCCCG 
    GCCGCTGCCC CTGTACCCGT GGCAGAGGAC GTGCCTCGGA CCTCTATGTG TTCCGTGCAG 
    TCGGCTCCGC CCACAGCAGC TCCAGTGAAG GGCATGGTGC CAGACGATGC TGTTGAAGCC 
    TTGGCTGGAA GCCTGGGCAA AAAGGAAGCA GATCCAGAAG ACGGAAAGCC TGTGGAGGAT 
    AAAGTCAAGG AAAAAGCCAA AGAAGAGGAT CGTGAGAAAC TTGGTGAAAA AGAAGAAACG 
    ATTCCTCCTG ATTACAGATT AGAAGAAGCC AAGGATAAAG ACGGAAAACC ACTGCTGCCA 
    AAAGAGGTCA AGGAACCGCT CCCACCCTTG AGTGAAGACG TCCTCCTCGA TGCTCTGTCC 
    AAGGACTTCA CTGTCCCCTC AGACACATCA TCGCCTCAAT TTGAAGATGC TAAACTTTCA 
    GCTGTCGTCT CTGAAGTGGT TTCCCAAACC CCAGCTCCAA CCACCCAGGC AGCCGGTCCA 
    CCCCCCAGCA CTGCGCGTGA CAACAAAGAA CTTGACGATG CCCTGGATCA ACTTTCTGAC 
    AGTCTCGGGC AAAGACAGCC TGATCCAGAT GAGAATAAAC CCGTAGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGATGACAC CATCCCACCT 
    AAATACCAAC ATCTTTTGGA TGACAACAAG GAGGGCACAC CCGGGAAGCC AAAGGCATCA 
    GAGAAGCCCA AGGCATCAGA GAAACCTGCA GGTGCCCAGG ACCCCATTGA TGCCCTCTCA 
    GGGGACTTTG ACAGCTGTCC CTCGACTACA GAAACCTCGA CAGACACACC AAAGGACAAA 
    GACAAGAAGC CTGCTTCCAG TGCCGAAGCA CCTAGGAATG GCGGGAAAGC AAAGGATTCC 
    ACAAAGGCAA AGGAGGAAAC TTCCAAGCCA AAAGCTGATG GAAAAAGTAC AAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_010807617.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805919.1 Bos taurus calpastatin (CAST), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OBa45056
    Clone ID Related Accession (Same CDS sequence) XM_010807617.1
    Accession Version XM_010807617.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805919.1
    CDS152..2482
    Translation

    Target ORF information:

    RefSeq Version XM_010807617.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010807617.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTATT CCAGGCAGCA AACAGCTGGA AGGACCGCAT 
    TCTCCTAACA AGAAAAGACA CAAAAAACAG GCTGTAAAAA CAGAACCTGA GAAGAAGCCA 
    CAATCATCTA AGCCATCTGT GGTTCATGAG AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
    CACACAGAGC CAAAAAGCCT ACCCAAGCAC TCATCAGATA CAGGAAGCAA GCATGCTCCT 
    AAGGAAAAAG CCGTTTCCAA ATCAAGTGAG CAGCCACCAT CAGAGAAATC AACAAAACCA 
    AAGACCAAGT CACAGGACAA GATCTCCGGT GGTGGAAAGA GCACTGTTCC TGCTGCTGCT 
    GCTGCAGCAT CTGCCGAACC AGCTGACAAG TCAGACATGG ACACTGCTCT GGATGACTTA 
    ATAGACACTT TAGGAGAACC TGAAGAGATG AAAGAAGATA ACACAACATA TACCGGACCG 
    GAAGTGTCGG ATCCAATGAG TTCTACCTAC ATAGAGGAAC TGGGTAAAAG AGAATCCACA 
    CTTCCTCCAA AATATAAGGA ACTTCTGAAT AAAGAAGAAG GGATCGCAGG GCCTCCTCCA 
    GACTCCTTGA AACCCCTGGG GCCCAATGAT GCCATCGATG CCTTGTCATC CGACTTCACC 
    TGCAGTTCCC CTACAGCTGA TGCAAAGAAA ACTGAGAAAG AGAAATCTAC AGAAGAGGCT 
    TTAAAAGCTC AGTCAGCTGG GGTGATCAGA AGTGCTGCTC CACCCCAAGA GAAAAAAAGG 
    AAAGTGGAAA AGGATGCCAT GACTGAGCAC GCCCTGGAGG CCCTGTCAGC CTCCCTGGGC 
    ACCCGGAAGC CGGAGCCGGA GCTCGACCCC AGCTCCATTA AGGAGGTCGA TGAGGCAAAA 
    GCCAAAGAAG AGAAAGTAAA GAAATGTGGT GAAGATGAGG AAACAGTCCC ATCGGAGTAC 
    AGATTAAAAC CGGCCACAGA TAAAGATGGA AAACCACTCT TGCCAGAGGC TGAAGAAAAA 
    CCCAAGCCCC TGAGTGAATC AGAACTCATC GATGAACTCT CAGAAGATTT TGACCAGTCT 
    AAGTGTAAAG AAAAACAATC TAAGCCAACT GAAAAAACAG AGGCATCCCC GGCCGCTGCC 
    CCTGTACCCG TGGCAGAGGA CGTGCCTCGG ACCTCTATGT GTTCCGTGCA GTCGGCTCCG 
    CCCACAGCAG CTCCAGTGAA GGGCATGGTG CCAGACGATG CTGTTGAAGC CTTGGCTGGA 
    AGCCTGGGCA AAAAGGAAGC AGATCCAGAA GACGGAAAGC CTGTGGAGGA TAAAGTCAAG 
    GAAAAAGCCA AAGAAGAGGA TCGTGAGAAA CTTGGTGAAA AAGAAGAAAC GATTCCTCCT 
    GATTACAGAT TAGAAGAAGC CAAGGATAAA GACGGAAAAC CACTGCTGCC AAAAGAGGTC 
    AAGGAACCGC TCCCACCCTT GAGTGAAGAC GTCCTCCTCG ATGCTCTGTC CAAGGACTTC 
    ACTGTCCCCT CAGACACATC ATCGCCTCAA TTTGAAGATG CTAAACTTTC AGCTGTCGTC 
    TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA ACCACCCAGG CAGCCGGTCC ACCCCCCAGC 
    ACTGCGCAGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CAST ( XM_010807618.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805920.1 Bos taurus calpastatin (CAST), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OBa45057
    Clone ID Related Accession (Same CDS sequence) XM_010807618.1
    Accession Version XM_010807618.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product calpastatin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTGTA AAAACAGAAC CTGAGAAGAA GCCACAATCA 
    TCTAAGCCAT CTGTGGTTCA TGAGAAAAAA ACCCAAGAAG TAAAGCCAAA GGAACACACA 
    GAGCCAAAAA GCCTACCCAA GCACTCATCA GATACAGGAA GCAAGCATGC TCCTAAGGAA 
    AAAGCCGTTT CCAAATCAAG TGAGCAGCCA CCATCAGAGA AATCAACAAA ACCAAAGACC 
    AAGTCACAGG ACAAGATCTC CGGTGGTGGA AAGAGCACTG TTCCTGCTGC TGCTGCTGCA 
    GCATCTGCCG AACCAGCTGA CAAGAATAAA GAAAATAAAT TGTTAACATC GGCCGTACCA 
    GCTGAATCTA AACCAAGTAA ACCATCTGGA AAGTCAGACA TGGACACTGC TCTGGATGAC 
    TTAATAGACA CTTTAGGAGA ACCTGAAGAG ATGAAAGAAG ATAACACAAC ATATACCGGA 
    CCGGAAGTGT CGGATCCAAT GAGTTCTACC TACATAGAGG AACTGGGTAA AAGAGAATCC 
    ACACTTCCTC CAAAATATAA GGAACTTCTG AATAAAGAAG AAGGGATCGC AGGGCCTCCT 
    CCAGACTCCT TGAAACCCCT GGGGCCCAAT GATGCCATCG ATGCCTTGTC ATCCGACTTC 
    ACCTGCAGTT CCCCTACAGC TGATGCAAAG AAAACTGAGA AAGAGAAATC TACAGAAGAG 
    GCTTTAAAAG CTCAGTCAGC TGGGGTGATC AGAAGTGCTG CTCCACCCCA AGAGAAAAAA 
    AGGAAAGTGG AAAAGGATGC CATGACTGAG CACGCCCTGG AGGCCCTGTC AGCCTCCCTG 
    GGCACCCGGA AGCCGGAGCC GGAGCTCGAC CCCAGCTCCA TTAAGGAGGT CGATGAGGCA 
    AAAGCCAAAG AAGAGAAAGT AAAGAAATGT GGTGAAGATG AGGAAACAGT CCCATCGGAG 
    TACAGATTAA AACCGGCCAC AGATAAAGAT GGAAAACCAC TCTTGCCAGA GGCTGAAGAA 
    AAACCCAAGC CCCTGAGTGA ATCAGAACTC ATCGATGAAC TCTCAGAAGA TTTTGACCAG 
    TCTAAGTGTA AAGAAAAACA ATCTAAGCCA ACTGAAAAAA CAGAGGCATC CCCGGCCGCT 
    GCCCCTGTAC CCGTGGCAGA GGACGTGCCT CGGACCTCTA TGTGTTCCGT GCAGTCGGCT 
    CCGCCCACAG CAGCTCCAGT GAAGGGCATG GTGCCAGACG ATGCTGTTGA AGCCTTGGCT 
    GGAAGCCTGG GCAAAAAGGA AGCAGATCCA GAAGACGGAA AGCCTGTGGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGAAGA GGATCGTGAG AAACTTGGTG AAAAAGAAGA AACGATTCCT 
    CCTGATTACA GATTAGAAGA AGCCAAGGAT AAAGACGGAA AACCACTGCT GCCAAAAGAG 
    GTCAAGGAAC CGCTCCCACC CTTGAGTGAA GACGTCCTCC TCGATGCTCT GTCCAAGGAC 
    TTCACTGTCC CCTCAGACAC ATCATCGCCT CAATTTGAAG ATGCTAAACT TTCAGCTGTC 
    GTCTCTGAAG TGGTTTCCCA AACCCCAGCT CCAACCACCC AGGCAGCCGG TCCACCCCCC 
    AGCACTGCGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805920.1
    CDS154..2484
    Translation

    Target ORF information:

    RefSeq Version XM_010807618.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calpastatin (CAST), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010807618.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CGGGCCCGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGCGC TGCCCGCCAC 
    ACCCAGGAGC ATGTCAGTAG AAAAACCAGT GGATCGCCTT CCAAGTCAGG AGAAAAGAAA 
    GGATCAGATG AGAAAAAAGC AACAAGCCTT GGGAGCAGTC AGCTCTCCAG AACTCAGGCT 
    GATGAAAAAG CCCTGGTCCC CAAGGTAACT ACTTCCTCTG CGTCAGCCAG CAAGTCTTCC 
    AGTATGAATC CCACAGAAGC CAAGGCTGTA AAAACAGAAC CTGAGAAGAA GCCACAATCA 
    TCTAAGCCAT CTGTGGTTCA TGAGAAAAAA ACCCAAGAAG TAAAGCCAAA GGAACACACA 
    GAGCCAAAAA GCCTACCCAA GCACTCATCA GATACAGGAA GCAAGCATGC TCCTAAGGAA 
    AAAGCCGTTT CCAAATCAAG TGAGCAGCCA CCATCAGAGA AATCAACAAA ACCAAAGACC 
    AAGTCACAGG ACAAGATCTC CGGTGGTGGA AAGAGCACTG TTCCTGCTGC TGCTGCTGCA 
    GCATCTGCCG AACCAGCTGA CAAGAATAAA GAAAATAAAT TGTTAACATC GGCCGTACCA 
    GCTGAATCTA AACCAAGTAA ACCATCTGGA AAGTCAGACA TGGACACTGC TCTGGATGAC 
    TTAATAGACA CTTTAGGAGA ACCTGAAGAG ATGAAAGAAG ATAACACAAC ATATACCGGA 
    CCGGAAGTGT CGGATCCAAT GAGTTCTACC TACATAGAGG AACTGGGTAA AAGAGAATCC 
    ACACTTCCTC CAAAATATAA GGAACTTCTG AATAAAGAAG AAGGGATCGC AGGGCCTCCT 
    CCAGACTCCT TGAAACCCCT GGGGCCCAAT GATGCCATCG ATGCCTTGTC ATCCGACTTC 
    ACCTGCAGTT CCCCTACAGC TGATGCAAAG AAAACTGAGA AAGAGAAATC TACAGAAGAG 
    GCTTTAAAAG CTCAGTCAGC TGGGGTGATC AGAAGTGCTG CTCCACCCCA AGAGAAAAAA 
    AGGAAAGTGG AAAAGGATGC CATGACTGAG CACGCCCTGG AGGCCCTGTC AGCCTCCCTG 
    GGCACCCGGA AGCCGGAGCC GGAGCTCGAC CCCAGCTCCA TTAAGGAGGT CGATGAGGCA 
    AAAGCCAAAG AAGAGAAAGT AAAGAAATGT GGTGAAGATG AGGAAACAGT CCCATCGGAG 
    TACAGATTAA AACCGGCCAC AGATAAAGAT GGAAAACCAC TCTTGCCAGA GGCTGAAGAA 
    AAACCCAAGC CCCTGAGTGA ATCAGAACTC ATCGATGAAC TCTCAGAAGA TTTTGACCAG 
    TCTAAGTGTA AAGAAAAACA ATCTAAGCCA ACTGAAAAAA CAGAGGCATC CCCGGCCGCT 
    GCCCCTGTAC CCGTGGCAGA GGACGTGCCT CGGACCTCTA TGTGTTCCGT GCAGTCGGCT 
    CCGCCCACAG CAGCTCCAGT GAAGGGCATG GTGCCAGACG ATGCTGTTGA AGCCTTGGCT 
    GGAAGCCTGG GCAAAAAGGA AGCAGATCCA GAAGACGGAA AGCCTGTGGA GGATAAAGTC 
    AAGGAAAAAG CCAAAGAAGA GGATCGTGAG AAACTTGGTG AAAAAGAAGA AACGATTCCT 
    CCTGATTACA GATTAGAAGA AGCCAAGGAT AAAGACGGAA AACCACTGCT GCCAAAAGAG 
    GTCAAGGAAC CGCTCCCACC CTTGAGTGAA GACGTCCTCC TCGATGCTCT GTCCAAGGAC 
    TTCACTGTCC CCTCAGACAC ATCATCGCCT CAATTTGAAG ATGCTAAACT TTCAGCTGTC 
    GTCTCTGAAG TGGTTTCCCA AACCCCAGCT CCAACCACCC AGGCAGCCGG TCCACCCCCC 
    AGCACTGCGC GTGACAACAA AGAACTTGAC GATGCCCTGG ATCAACTTTC TGACAGTCTC 
    GGGCAAAGAC AGCCTGATCC AGATGAGAAT AAACCCGTAG AGGATAAAGT CAAGGAAAAA 
    GCCAAAGCTG AACACAGAGA CAAGCTGGGA GAAAGAGATG ACACCATCCC ACCTAAATAC 
    CAACATCTTT TGGATGACAA CAAGGAGGGC ACACCCGGGA AGCCAAAGGC ATCAGAGAAG 
    CCCAAGGCAT CAGAGAAACC TGCAGGTGCC CAGGACCCCA TTGATGCCCT CTCAGGGGAC 
    TTTGACAGCT GTCCCTCGAC TACAGAAACC TCGACAGACA CACCAAAGGA CAAAGACAAG 
    AAGCCTGCTT CCAGTGCCGA AGCACCTAGG AATGGCGGGA AAGCAAAGGA TTCCACAAAG 
    GCAAAGGAGG AAACTTCCAA GCCAAAAGCT GATGGAAAAA GTACAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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