CAST cDNA ORF clone, Gallus gallus(chicken)

The following CAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa00493 NM_001137650.1
Latest version!
Gallus gallus calpastatin (CAST), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OGa152979 XM_025144706.1
Latest version!
Gallus gallus calpastatin (CAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa48193 XM_015280523.1
Latest version!
Gallus gallus calpastatin (CAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa48194 XM_015280524.1
Latest version!
Gallus gallus calpastatin (CAST), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa48195 XM_015280525.1
Latest version!
Gallus gallus calpastatin (CAST), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa48196 XM_015280526.1
Latest version!
Gallus gallus calpastatin (CAST), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa48197 XM_015280527.1
Latest version!
Gallus gallus calpastatin (CAST), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa48198 XM_015280528.1
Latest version!
Gallus gallus calpastatin (CAST), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa48199 XM_015280529.2
Latest version!
Gallus gallus calpastatin (CAST), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OGa48199 XM_015280529.1 Gallus gallus calpastatin (CAST), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa00493
    Clone ID Related Accession (Same CDS sequence) NM_001137650.1
    Accession Version NM_001137650.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-20
    Organism Gallus gallus(chicken)
    Product calpastatin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ232592.1. On Nov 14, 2008 this sequence version replaced XM_424713.2. ##Evidence-Data-START## Transcript exon combination :: FJ232592.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAATGGTG TCCCCTATGG GTCCAATGGT GTCCTATGGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT
    CCCTCTGCGG ATGCACCAAA ACCACCCACT ATGAAGCCAT CTGAAGCAAA GTCCACACCA
    ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT
    AATCAACCGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT
    TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG
    CGGCCTTCAC AGGCAACCTC TGCAGACACC GCACAGCCTA AGACTGAAAA GACATCCACA
    GCAGGCGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCACGA GTATGGTTGC TGCAGCTGAT
    AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA
    GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA
    AATATTTCTT CAGAATACTT GGAAGAGCTG GGAAAACGAG AAGGTAGCCT CCCTCCAGAG
    TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT
    GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT
    ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA
    GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG
    AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT
    GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG
    GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA
    GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA
    GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC
    AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG
    CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT
    TGCTCTGTGC AGTCTTCGGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA
    GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA
    CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA
    GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA
    CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA
    GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA
    AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA
    ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT
    GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC
    AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA
    GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT
    AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA
    GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT
    GCCACACCAC AGCACTCAAA GGAGAAAAGT GGAAAGGAAA CCGTCACCAC TAAAGCAACC
    CCAAAGGATG AAAGAAAACC CAAGGATCAT AAAAAGGCAA GGGGACAGTC CTCCAGCAGC
    AAATCTGAGA AGCAGAAAAC AAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001131122.1
    CDS1..2307
    Translation

    Target ORF information:

    RefSeq Version NM_001137650.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001137650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAATGGTG TCCCCTATGG GTCCAATGGT GTCCTATGGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT
    CCCTCTGCGG ATGCACCAAA ACCACCCACT ATGAAGCCAT CTGAAGCAAA GTCCACACCA
    ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT
    AATCAACCGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT
    TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG
    CGGCCTTCAC AGGCAACCTC TGCAGACACC GCACAGCCTA AGACTGAAAA GACATCCACA
    GCAGGCGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCACGA GTATGGTTGC TGCAGCTGAT
    AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA
    GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA
    AATATTTCTT CAGAATACTT GGAAGAGCTG GGAAAACGAG AAGGTAGCCT CCCTCCAGAG
    TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT
    GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT
    ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA
    GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG
    AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT
    GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG
    GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA
    GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA
    GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC
    AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG
    CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT
    TGCTCTGTGC AGTCTTCGGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA
    GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA
    CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA
    GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA
    CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA
    GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA
    AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA
    ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT
    GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC
    AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA
    GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT
    AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA
    GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT
    GCCACACCAC AGCACTCAAA GGAGAAAAGT GGAAAGGAAA CCGTCACCAC TAAAGCAACC
    CCAAAGGATG AAAGAAAACC CAAGGATCAT AAAAAGGCAA GGGGACAGTC CTCCAGCAGC
    AAATCTGAGA AGCAGAAAAC AAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152979
    Clone ID Related Accession (Same CDS sequence) XM_025144706.1
    Accession Version XM_025144706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGACCCCG ACACCCCCCC CCCCCCGTGC CCGGCCCGCA GTCCCGCCGG CACCGCCCTG 
    CTGCGGGGCT CGGTGGCGGC TGGGGCGCGG CCGTGGCCAC TCCCGTGGCT GGTGCCGCCC
    TCAGAGGGAG GTGGCTGCTG GGGCGCGGCC CGTGCGTCTG TGTCCCTTGC CGTGCCGTGC
    CGTGCCGTGC CGTGCGGAGC AGCGAGCAGC GAGCAGCGCC ATGGCTTTCG CGCGCTGGTG
    CAGGGCGACA AGAAGGCAAG TGGAACTTCT TCCAAATCGG GAGAGAAAAA AGAAGCAACA
    GAGAAGCCAG CAGGTGCTAA TGTCAGTCAG CTTCCCAAAA CACAGACAAG TGGAGTACTG
    CCTGCTGCTA AGAAGCAGTC TCCCTCTGCG GATGCACCAA AACCACCCAT TATGAAGCCA
    TCTGAAGCAA AGTCCACACC AACAATCCAA ACAGAACCTG AGAAAACTGC GCTGTCAACA
    AAGCCATCAA ACTCTCAAAA TAATCAACTG GCTGAAGAGA AGCCAAAGGA ATCCAGTGGT
    GCAAAAAGCC CAATTGCACC TTCTGTGGTA ACAGCTTCTA AACCAAATAA TATGGGAAAG
    GAAACTACAA GTCAAGCTGA GCGGCCTTCA CAGGCAACCT CTGCAGACAC AGCACAGCCT
    AAGACTGAAA AGACATCCAC AGCAGGTGGT GCAGGTGCAG CAGGTGCAGG TGCTGTCGCG
    AGTATGGTTG CTGCAGCTGA TAAACCAAAT AGTGAGCCTG CTATGGATGA ATCAGCACTG
    GACAGCTTAA TAGATACGCT AGGTGGATCT GAAGAAGATG TGGCTACTAG ACCAGTTTAC
    ACTGGTCCGG AGATTACGGA AAATATTTCT TCAGAATACT TGGAAGAGCT GGGTAAACGA
    GAAGGTAGCC TCCCTCCAGA GTACCTTGCA TTATTGAAGA GCAAAGTGGA TGGCAAAGAT
    GGAGGCCTAC CAAAAGTAGA TGAACATTCT GAAAAACCGA TGACGGATGA TGAGCTTGCA
    GATGCCCTGT CATCTGACTT TACCTGTAGC ATTGCTGCTG CTGAAGAAAA GAAGACAACA
    GAGAAACAAA CTAAGGAAGG AGAAATCATA CAAGCACAAG TGACAAGTTC AGTGAAGACT
    TCAGTTCCTC CTAAAGAGAA GAAAACAAAA TCAAAAGAGG AAATCAAAGA AGATGCAATG
    GAAGCTCTCA TGGCTACTAT TGGTGGACCT GAACCTGAAC CCGAACCAAA AGATGTTAGC
    CCTATTGTAG AAGTGTCAGA GGCAAAAGCT AAAGAGAAAA AAGAAAAGAA GGCTGGAGAA
    CGTGATGATA CAATACCTCC AGAATACAGG CTAACTCCAG AGCTGGATAA AGATGGAAAA
    CCAGTATTGC CAAAGCCTGA AGAAAAACCT AAGCCTTTGA GTGAATCTGA CCTTGTTGAT
    GAATTTTCAA AAGACTTTGC CAGCCCTGCA CAGCCTGCAG TACAATCCAA ACCATCAAAG
    CCCAGCAACA CATCCAAAAA GCCTTCAGCT CCTGCATCTA CAAAAACTGC AAAGGATGAA
    GTAGTCCCTC GTGCCACAGC TTGCTCTGTG CAGTCTTCAG CTCCAACATC TGTGTCTTCT
    GTTGGTCACG TAGCTGATGA AGCAGTAGAA GCCTTGTCCA GTAGTCTGGG AGAAAGGGAG
    CCTGATCCAG AAGAAAAGAA ACCTGCTGTG GACAAAGTTA AGGAGAAAGC CAAACGGAAA
    CAACACAAAA AACTGGGTGA AGATGAAGAA ACAATTCCTC CAGAGTACAG ATTAACAGAT
    GCAAAAGATA AAGATGGAAG ACCACTGTTA ATAAAACCTG AAGAGGAGTC CCAGCCTATG
    AGTGAAAACG ATCTGTTAGA AGGTTTGACA AAGGGATTTT CTCCAGCCCA ATCTGCACCA
    TTACCTACAC AAACTGTAAA AAAGAAAACC AAAGGGGGTA AAAAAACTGC AGATTCTTCA
    GATGTTATCT CTGCTTCTAC AATTTCCTCA GTGCACTCAG CAGCTCCCCT AGCTTCTACG
    TCGGGAGGAA AAGAGATGGA TGATGCCTTG GATCTCTTGT CTGATTCCCT GGGACAGAGA
    GAACCTGACC CTGATGAGAA CAAACCAGTT GTGGATAAAG TAAAGGAAAA AGCTAAGTCT
    GAACACAGAG ACAAACTTGG AGAAAGAGAT GATACTATTC CACCTGACTA TCGAAAACTC
    CTGGAGTCAT CTGATGAGGG TAAACCTGTC AAGCCAACAC CAAAGGATGC TTCGAAAAAC
    AAAGAACAAA AGAAGCCTAC AGATGAGTCT GCTGCTATTG ATGCCCTGTC AGGCGACTTT
    GATACTAGTC CAAAGGCTCC TGCCACACCA CAGCACTCAA AGGAGAAAAG TGGAAAGGAA
    ACCGTCACCA CTAAAGCAAC CCCAAAGGAT GAAAGAAAAC CCAAGGATCA TAAAAAGGCA
    AGGGGACAGT CCTCCAGCAG CAAATCTGAG AAGCAGAAAA CAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000474.1
    CDS16..2523
    Translation

    Target ORF information:

    RefSeq Version XM_025144706.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGACCCCG ACACCCCCCC CCCCCCGTGC CCGGCCCGCA GTCCCGCCGG CACCGCCCTG 
    CTGCGGGGCT CGGTGGCGGC TGGGGCGCGG CCGTGGCCAC TCCCGTGGCT GGTGCCGCCC
    TCAGAGGGAG GTGGCTGCTG GGGCGCGGCC CGTGCGTCTG TGTCCCTTGC CGTGCCGTGC
    CGTGCCGTGC CGTGCGGAGC AGCGAGCAGC GAGCAGCGCC ATGGCTTTCG CGCGCTGGTG
    CAGGGCGACA AGAAGGCAAG TGGAACTTCT TCCAAATCGG GAGAGAAAAA AGAAGCAACA
    GAGAAGCCAG CAGGTGCTAA TGTCAGTCAG CTTCCCAAAA CACAGACAAG TGGAGTACTG
    CCTGCTGCTA AGAAGCAGTC TCCCTCTGCG GATGCACCAA AACCACCCAT TATGAAGCCA
    TCTGAAGCAA AGTCCACACC AACAATCCAA ACAGAACCTG AGAAAACTGC GCTGTCAACA
    AAGCCATCAA ACTCTCAAAA TAATCAACTG GCTGAAGAGA AGCCAAAGGA ATCCAGTGGT
    GCAAAAAGCC CAATTGCACC TTCTGTGGTA ACAGCTTCTA AACCAAATAA TATGGGAAAG
    GAAACTACAA GTCAAGCTGA GCGGCCTTCA CAGGCAACCT CTGCAGACAC AGCACAGCCT
    AAGACTGAAA AGACATCCAC AGCAGGTGGT GCAGGTGCAG CAGGTGCAGG TGCTGTCGCG
    AGTATGGTTG CTGCAGCTGA TAAACCAAAT AGTGAGCCTG CTATGGATGA ATCAGCACTG
    GACAGCTTAA TAGATACGCT AGGTGGATCT GAAGAAGATG TGGCTACTAG ACCAGTTTAC
    ACTGGTCCGG AGATTACGGA AAATATTTCT TCAGAATACT TGGAAGAGCT GGGTAAACGA
    GAAGGTAGCC TCCCTCCAGA GTACCTTGCA TTATTGAAGA GCAAAGTGGA TGGCAAAGAT
    GGAGGCCTAC CAAAAGTAGA TGAACATTCT GAAAAACCGA TGACGGATGA TGAGCTTGCA
    GATGCCCTGT CATCTGACTT TACCTGTAGC ATTGCTGCTG CTGAAGAAAA GAAGACAACA
    GAGAAACAAA CTAAGGAAGG AGAAATCATA CAAGCACAAG TGACAAGTTC AGTGAAGACT
    TCAGTTCCTC CTAAAGAGAA GAAAACAAAA TCAAAAGAGG AAATCAAAGA AGATGCAATG
    GAAGCTCTCA TGGCTACTAT TGGTGGACCT GAACCTGAAC CCGAACCAAA AGATGTTAGC
    CCTATTGTAG AAGTGTCAGA GGCAAAAGCT AAAGAGAAAA AAGAAAAGAA GGCTGGAGAA
    CGTGATGATA CAATACCTCC AGAATACAGG CTAACTCCAG AGCTGGATAA AGATGGAAAA
    CCAGTATTGC CAAAGCCTGA AGAAAAACCT AAGCCTTTGA GTGAATCTGA CCTTGTTGAT
    GAATTTTCAA AAGACTTTGC CAGCCCTGCA CAGCCTGCAG TACAATCCAA ACCATCAAAG
    CCCAGCAACA CATCCAAAAA GCCTTCAGCT CCTGCATCTA CAAAAACTGC AAAGGATGAA
    GTAGTCCCTC GTGCCACAGC TTGCTCTGTG CAGTCTTCAG CTCCAACATC TGTGTCTTCT
    GTTGGTCACG TAGCTGATGA AGCAGTAGAA GCCTTGTCCA GTAGTCTGGG AGAAAGGGAG
    CCTGATCCAG AAGAAAAGAA ACCTGCTGTG GACAAAGTTA AGGAGAAAGC CAAACGGAAA
    CAACACAAAA AACTGGGTGA AGATGAAGAA ACAATTCCTC CAGAGTACAG ATTAACAGAT
    GCAAAAGATA AAGATGGAAG ACCACTGTTA ATAAAACCTG AAGAGGAGTC CCAGCCTATG
    AGTGAAAACG ATCTGTTAGA AGGTTTGACA AAGGGATTTT CTCCAGCCCA ATCTGCACCA
    TTACCTACAC AAACTGTAAA AAAGAAAACC AAAGGGGGTA AAAAAACTGC AGATTCTTCA
    GATGTTATCT CTGCTTCTAC AATTTCCTCA GTGCACTCAG CAGCTCCCCT AGCTTCTACG
    TCGGGAGGAA AAGAGATGGA TGATGCCTTG GATCTCTTGT CTGATTCCCT GGGACAGAGA
    GAACCTGACC CTGATGAGAA CAAACCAGTT GTGGATAAAG TAAAGGAAAA AGCTAAGTCT
    GAACACAGAG ACAAACTTGG AGAAAGAGAT GATACTATTC CACCTGACTA TCGAAAACTC
    CTGGAGTCAT CTGATGAGGG TAAACCTGTC AAGCCAACAC CAAAGGATGC TTCGAAAAAC
    AAAGAACAAA AGAAGCCTAC AGATGAGTCT GCTGCTATTG ATGCCCTGTC AGGCGACTTT
    GATACTAGTC CAAAGGCTCC TGCCACACCA CAGCACTCAA AGGAGAAAAG TGGAAAGGAA
    ACCGTCACCA CTAAAGCAAC CCCAAAGGAT GAAAGAAAAC CCAAGGATCA TAAAAAGGCA
    AGGGGACAGT CCTCCAGCAG CAAATCTGAG AAGCAGAAAA CAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48193
    Clone ID Related Accession (Same CDS sequence) XM_015280523.1
    Accession Version XM_015280523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT
    CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA
    ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT
    AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT
    TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG
    CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA
    GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT
    AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA
    GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA
    AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG
    TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT
    GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT
    ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA
    GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG
    AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT
    GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG
    GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA
    GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA
    GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC
    AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG
    CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT
    TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA
    GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA
    CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA
    GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA
    CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA
    GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA
    AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA
    ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT
    GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC
    AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA
    GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT
    AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA
    GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT
    GCCACACCAC AGCACTCAAA GTGTAGGAGA AAAGTGGAAA GGAAACCGTC ACCACTAAAG
    CAACCCCAAA GGATGAAAGA AAACCCAAGG ATCATAAAAA GGCAAGGGGA CAGTCCTCCA
    GCAGCAAATC TGAGAAGCAG AAAACAAGCT AAACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136009.1
    CDS95..2413
    Translation

    Target ORF information:

    RefSeq Version XM_015280523.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT
    CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA
    ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT
    AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT
    TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG
    CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA
    GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT
    AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA
    GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA
    AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG
    TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT
    GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT
    ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA
    GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG
    AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT
    GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG
    GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA
    GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA
    GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC
    AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG
    CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT
    TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA
    GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA
    CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA
    GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA
    CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA
    GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA
    AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA
    ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT
    GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC
    AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA
    GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT
    AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA
    GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT
    GCCACACCAC AGCACTCAAA GTGTAGGAGA AAAGTGGAAA GGAAACCGTC ACCACTAAAG
    CAACCCCAAA GGATGAAAGA AAACCCAAGG ATCATAAAAA GGCAAGGGGA CAGTCCTCCA
    GCAGCAAATC TGAGAAGCAG AAAACAAGCT AAACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48194
    Clone ID Related Accession (Same CDS sequence) XM_015280524.1
    Accession Version XM_015280524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GCAGTCTCCC
    TCTGCGGATG CACCAAAACC ACCCATTATG AAGCCATCTG AAGCAAAGTC CACACCAACA
    ATCCAAACAG AACCTGAGAA AACTGCGCTG TCAACAAAGC CATCAAACTC TCAAAATAAT
    CAACTGGCTG AAGAGAAGCC AAAGGAATCC AGTGGTGCAA AAAGCCCAAT TGCACCTTCT
    GTGGTAACAG CTTCTAAACC AAATAATATG GGAAAGGAAA CTACAAGTCA AGCTGAGCGG
    CCTTCACAGG CAACCTCTGC AGACACAGCA CAGCCTAAGA CTGAAAAGAC ATCCACAGCA
    GGTGGTGCAG GTGCAGCAGG TGCAGGTGCT GTCGCGAGTA TGGTTGCTGC AGCTGATAAA
    CCAAATAGTG AGCCTGCTAT GGATGAATCA GCACTGGACA GCTTAATAGA TACGCTAGGT
    GGATCTGAAG AAGATGTGGC TACTAGACCA GTTTACACTG GTCCGGAGAT TACGGAAAAT
    ATTTCTTCAG AATACTTGGA AGAGCTGGGT AAACGAGAAG GTAGCCTCCC TCCAGAGTAC
    CTTGCATTAT TGAAGAGCAA AGTGGATGGC AAAGATGGAG GCCTACCAAA AGTAGATGAA
    CATTCTGAAA AACCGATGAC GGATGATGAG CTTGCAGATG CCCTGTCATC TGACTTTACC
    TGTAGCATTG CTGCTGCTGA AGAAAAGAAG ACAACAGAGA AACAAACTAA GGAAGGAGAA
    ATCATACAAG CACAAGTGAC AAGTTCAGTG AAGACTTCAG TTCCTCCTAA AGAGAAGAAA
    ACAAAATCAA AAGAGGAAAT CAAAGAAGAT GCAATGGAAG CTCTCATGGC TACTATTGGT
    GGACCTGAAC CTGAACCCGA ACCAAAAGAT GTTAGCCCTA TTGTAGAAGT GTCAGAGGCA
    AAAGCTAAAG AGAAAAAAGA AAAGAAGGCT GGAGAACGTG ATGATACAAT ACCTCCAGAA
    TACAGGCTAA CTCCAGAGCT GGATAAAGAT GGAAAACCAG TATTGCCAAA GCCTGAAGAA
    AAACCTAAGC CTTTGAGTGA ATCTGACCTT GTTGATGAAT TTTCAAAAGA CTTTGCCAGC
    CCTGCACAGC CTGCAGTACA ATCCAAACCA TCAAAGCCCA GCAACACATC CAAAAAGCCT
    TCAGCTCCTG CATCTACAAA AACTGCAAAG GATGAAGTAG TCCCTCGTGC CACAGCTTGC
    TCTGTGCAGT CTTCAGCTCC AACATCTGTG TCTTCTGTTG GTCACGTAGC TGATGAAGCA
    GTAGAAGCCT TGTCCAGTAG TCTGGGAGAA AGGGAGCCTG ATCCAGAAGA AAAGAAACCT
    GCTGTGGACA AAGTTAAGGA GAAAGCCAAA CGGAAACAAC ACAAAAAACT GGGTGAAGAT
    GAAGAAACAA TTCCTCCAGA GTACAGATTA ACAGATGCAA AAGATAAAGA TGGAAGACCA
    CTGTTAATAA AACCTGAAGA GGAGTCCCAG CCTATGAGTG AAAACGATCT GTTAGAAGGT
    TTGACAAAGG GATTTTCTCC AGCCCAATCT GCACCATTAC CTACACAAAC TGTAAAAAAG
    AAAACCAAAG GGGGTAAAAA AACTGCAGAT TCTTCAGATG TTATCTCTGC TTCTACAATT
    TCCTCAGTGC ACTCAGCAGC TCCCCTAGCT TCTACGTCGG GAGGAAAAGA GATGGATGAT
    GCCTTGGATC TCTTGTCTGA TTCCCTGGGA CAGAGAGAAC CTGACCCTGA TGAGAACAAA
    CCAGTTGTGG ATAAAGTAAA GGAAAAAGCT AAGTCTGAAC ACAGAGACAA ACTTGGAGAA
    AGAGATGATA CTATTCCACC TGACTATCGA AAACTCCTGG AGTCATCTGA TGAGGGTAAA
    CCTGTCAAGC CAACACCAAA GGATGCTTCG AAAAACAAAG AACAAAAGAA GCCTACAGAT
    GAGTCTGCTG CTATTGATGC CCTGTCAGGC GACTTTGATA CTAGTCCAAA GGCTCCTGCC
    ACACCACAGC ACTCAAAGTG TAGGAGAAAA GTGGAAAGGA AACCGTCACC ACTAAAGCAA
    CCCCAAAGGA TGAAAGAAAA CCCAAGGATC ATAAAAAGGC AAGGGGACAG TCCTCCAGCA
    GCAAATCTGA GAAGCAGAAA ACAAGCTAAA CGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136010.1
    CDS95..2410
    Translation

    Target ORF information:

    RefSeq Version XM_015280524.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GCAGTCTCCC
    TCTGCGGATG CACCAAAACC ACCCATTATG AAGCCATCTG AAGCAAAGTC CACACCAACA
    ATCCAAACAG AACCTGAGAA AACTGCGCTG TCAACAAAGC CATCAAACTC TCAAAATAAT
    CAACTGGCTG AAGAGAAGCC AAAGGAATCC AGTGGTGCAA AAAGCCCAAT TGCACCTTCT
    GTGGTAACAG CTTCTAAACC AAATAATATG GGAAAGGAAA CTACAAGTCA AGCTGAGCGG
    CCTTCACAGG CAACCTCTGC AGACACAGCA CAGCCTAAGA CTGAAAAGAC ATCCACAGCA
    GGTGGTGCAG GTGCAGCAGG TGCAGGTGCT GTCGCGAGTA TGGTTGCTGC AGCTGATAAA
    CCAAATAGTG AGCCTGCTAT GGATGAATCA GCACTGGACA GCTTAATAGA TACGCTAGGT
    GGATCTGAAG AAGATGTGGC TACTAGACCA GTTTACACTG GTCCGGAGAT TACGGAAAAT
    ATTTCTTCAG AATACTTGGA AGAGCTGGGT AAACGAGAAG GTAGCCTCCC TCCAGAGTAC
    CTTGCATTAT TGAAGAGCAA AGTGGATGGC AAAGATGGAG GCCTACCAAA AGTAGATGAA
    CATTCTGAAA AACCGATGAC GGATGATGAG CTTGCAGATG CCCTGTCATC TGACTTTACC
    TGTAGCATTG CTGCTGCTGA AGAAAAGAAG ACAACAGAGA AACAAACTAA GGAAGGAGAA
    ATCATACAAG CACAAGTGAC AAGTTCAGTG AAGACTTCAG TTCCTCCTAA AGAGAAGAAA
    ACAAAATCAA AAGAGGAAAT CAAAGAAGAT GCAATGGAAG CTCTCATGGC TACTATTGGT
    GGACCTGAAC CTGAACCCGA ACCAAAAGAT GTTAGCCCTA TTGTAGAAGT GTCAGAGGCA
    AAAGCTAAAG AGAAAAAAGA AAAGAAGGCT GGAGAACGTG ATGATACAAT ACCTCCAGAA
    TACAGGCTAA CTCCAGAGCT GGATAAAGAT GGAAAACCAG TATTGCCAAA GCCTGAAGAA
    AAACCTAAGC CTTTGAGTGA ATCTGACCTT GTTGATGAAT TTTCAAAAGA CTTTGCCAGC
    CCTGCACAGC CTGCAGTACA ATCCAAACCA TCAAAGCCCA GCAACACATC CAAAAAGCCT
    TCAGCTCCTG CATCTACAAA AACTGCAAAG GATGAAGTAG TCCCTCGTGC CACAGCTTGC
    TCTGTGCAGT CTTCAGCTCC AACATCTGTG TCTTCTGTTG GTCACGTAGC TGATGAAGCA
    GTAGAAGCCT TGTCCAGTAG TCTGGGAGAA AGGGAGCCTG ATCCAGAAGA AAAGAAACCT
    GCTGTGGACA AAGTTAAGGA GAAAGCCAAA CGGAAACAAC ACAAAAAACT GGGTGAAGAT
    GAAGAAACAA TTCCTCCAGA GTACAGATTA ACAGATGCAA AAGATAAAGA TGGAAGACCA
    CTGTTAATAA AACCTGAAGA GGAGTCCCAG CCTATGAGTG AAAACGATCT GTTAGAAGGT
    TTGACAAAGG GATTTTCTCC AGCCCAATCT GCACCATTAC CTACACAAAC TGTAAAAAAG
    AAAACCAAAG GGGGTAAAAA AACTGCAGAT TCTTCAGATG TTATCTCTGC TTCTACAATT
    TCCTCAGTGC ACTCAGCAGC TCCCCTAGCT TCTACGTCGG GAGGAAAAGA GATGGATGAT
    GCCTTGGATC TCTTGTCTGA TTCCCTGGGA CAGAGAGAAC CTGACCCTGA TGAGAACAAA
    CCAGTTGTGG ATAAAGTAAA GGAAAAAGCT AAGTCTGAAC ACAGAGACAA ACTTGGAGAA
    AGAGATGATA CTATTCCACC TGACTATCGA AAACTCCTGG AGTCATCTGA TGAGGGTAAA
    CCTGTCAAGC CAACACCAAA GGATGCTTCG AAAAACAAAG AACAAAAGAA GCCTACAGAT
    GAGTCTGCTG CTATTGATGC CCTGTCAGGC GACTTTGATA CTAGTCCAAA GGCTCCTGCC
    ACACCACAGC ACTCAAAGTG TAGGAGAAAA GTGGAAAGGA AACCGTCACC ACTAAAGCAA
    CCCCAAAGGA TGAAAGAAAA CCCAAGGATC ATAAAAAGGC AAGGGGACAG TCCTCCAGCA
    GCAAATCTGA GAAGCAGAAA ACAAGCTAAA CGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48195
    Clone ID Related Accession (Same CDS sequence) XM_015280525.1
    Accession Version XM_015280525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT
    CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA
    ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT
    AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT
    TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG
    CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA
    GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT
    AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA
    GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA
    AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG
    TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT
    GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT
    ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA
    GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG
    AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT
    GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG
    GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA
    GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA
    GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC
    AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG
    CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT
    TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA
    GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA
    CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA
    GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA
    CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA
    GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA
    AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA
    ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT
    GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC
    AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA
    GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT
    AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA
    GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT
    GCCACACCAC AGCACTCAAA GGAGAAAAGT GGAAAGGAAA CCGTCACCAC TAAAGCAACC
    CCAAAGGATG AAAGAAAACC CAAGGATCAT AAAAAGGCAA GGGGACAGTC CTCCAGCAGC
    AAATCTGAGA AGCAGAAAAC AAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136011.1
    CDS95..2401
    Translation

    Target ORF information:

    RefSeq Version XM_015280525.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT
    CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA
    ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT
    AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT
    TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG
    CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA
    GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT
    AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA
    GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA
    AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG
    TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT
    GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT
    ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA
    GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG
    AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT
    GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG
    GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA
    GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA
    GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC
    AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG
    CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT
    TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA
    GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA
    CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA
    GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA
    CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA
    GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA
    AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA
    ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT
    GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC
    AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA
    GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT
    AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA
    GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT
    GCCACACCAC AGCACTCAAA GGAGAAAAGT GGAAAGGAAA CCGTCACCAC TAAAGCAACC
    CCAAAGGATG AAAGAAAACC CAAGGATCAT AAAAAGGCAA GGGGACAGTC CTCCAGCAGC
    AAATCTGAGA AGCAGAAAAC AAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48196
    Clone ID Related Accession (Same CDS sequence) XM_015280526.1
    Accession Version XM_015280526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCAGTC TCCCTCTGCG
    GATGCACCAA AACCACCCAT TATGAAGCCA TCTGAAGCAA AGTCCACACC AACAATCCAA
    ACAGAACCTG AGAAAACTGC GCTGTCAACA AAGCCATCAA ACTCTCAAAA TAATCAACTG
    GCTGAAGAGA AGCCAAAGGA ATCCAGTGGT GCAAAAAGCC CAATTGCACC TTCTGTGGTA
    ACAGCTTCTA AACCAAATAA TATGGGAAAG GAAACTACAA GTCAAGCTGA GCGGCCTTCA
    CAGGCAACCT CTGCAGACAC AGCACAGCCT AAGACTGAAA AGACATCCAC AGCAGGTGGT
    GCAGGTGCAG CAGGTGCAGG TGCTGTCGCG AGTATGGTTG CTGCAGCTGA TAAACCAAAT
    AGTGAGCCTG CTATGGATGA ATCAGCACTG GACAGCTTAA TAGATACGCT AGGTGGATCT
    GAAGAAGATG TGGCTACTAG ACCAGTTTAC ACTGGTCCGG AGATTACGGA AAATATTTCT
    TCAGAATACT TGGAAGAGCT GGGTAAACGA GAAGGTAGCC TCCCTCCAGA GTACCTTGCA
    TTATTGAAGA GCAAAGTGGA TGGCAAAGAT GGAGGCCTAC CAAAAGTAGA TGAACATTCT
    GAAAAACCGA TGACGGATGA TGAGCTTGCA GATGCCCTGT CATCTGACTT TACCTGTAGC
    ATTGCTGCTG CTGAAGAAAA GAAGACAACA GAGAAACAAA CTAAGGAAGG AGAAATCATA
    CAAGCACAAG TGACAAGTTC AGTGAAGACT TCAGTTCCTC CTAAAGAGAA GAAAACAAAA
    TCAAAAGAGG AAATCAAAGA AGATGCAATG GAAGCTCTCA TGGCTACTAT TGGTGGACCT
    GAACCTGAAC CCGAACCAAA AGATGTTAGC CCTATTGTAG AAGTGTCAGA GGCAAAAGCT
    AAAGAGAAAA AAGAAAAGAA GGCTGGAGAA CGTGATGATA CAATACCTCC AGAATACAGG
    CTAACTCCAG AGCTGGATAA AGATGGAAAA CCAGTATTGC CAAAGCCTGA AGAAAAACCT
    AAGCCTTTGA GTGAATCTGA CCTTGTTGAT GAATTTTCAA AAGACTTTGC CAGCCCTGCA
    CAGCCTGCAG TACAATCCAA ACCATCAAAG CCCAGCAACA CATCCAAAAA GCCTTCAGCT
    CCTGCATCTA CAAAAACTGC AAAGGATGAA GTAGTCCCTC GTGCCACAGC TTGCTCTGTG
    CAGTCTTCAG CTCCAACATC TGTGTCTTCT GTTGGTCACG TAGCTGATGA AGCAGTAGAA
    GCCTTGTCCA GTAGTCTGGG AGAAAGGGAG CCTGATCCAG AAGAAAAGAA ACCTGCTGTG
    GACAAAGTTA AGGAGAAAGC CAAACGGAAA CAACACAAAA AACTGGGTGA AGATGAAGAA
    ACAATTCCTC CAGAGTACAG ATTAACAGAT GCAAAAGATA AAGATGGAAG ACCACTGTTA
    ATAAAACCTG AAGAGGAGTC CCAGCCTATG AGTGAAAACG ATCTGTTAGA AGGTTTGACA
    AAGGGATTTT CTCCAGCCCA ATCTGCACCA TTACCTACAC AAACTGTAAA AAAGAAAACC
    AAAGGGGGTA AAAAAACTGC AGATTCTTCA GATGTTATCT CTGCTTCTAC AATTTCCTCA
    GTGCACTCAG CAGCTCCCCT AGCTTCTACG TCGGGAGGAA AAGAGATGGA TGATGCCTTG
    GATCTCTTGT CTGATTCCCT GGGACAGAGA GAACCTGACC CTGATGAGAA CAAACCAGTT
    GTGGATAAAG TAAAGGAAAA AGCTAAGTCT GAACACAGAG ACAAACTTGG AGAAAGAGAT
    GATACTATTC CACCTGACTA TCGAAAACTC CTGGAGTCAT CTGATGAGGG TAAACCTGTC
    AAGCCAACAC CAAAGGATGC TTCGAAAAAC AAAGAACAAA AGAAGCCTAC AGATGAGTCT
    GCTGCTATTG ATGCCCTGTC AGGCGACTTT GATACTAGTC CAAAGGCTCC TGCCACACCA
    CAGCACTCAA AGTGTAGGAG AAAAGTGGAA AGGAAACCGT CACCACTAAA GCAACCCCAA
    AGGATGAAAG AAAACCCAAG GATCATAAAA AGGCAAGGGG ACAGTCCTCC AGCAGCAAAT
    CTGAGAAGCA GAAAACAAGC TAAACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136012.1
    CDS95..2344
    Translation

    Target ORF information:

    RefSeq Version XM_015280526.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCAGTC TCCCTCTGCG
    GATGCACCAA AACCACCCAT TATGAAGCCA TCTGAAGCAA AGTCCACACC AACAATCCAA
    ACAGAACCTG AGAAAACTGC GCTGTCAACA AAGCCATCAA ACTCTCAAAA TAATCAACTG
    GCTGAAGAGA AGCCAAAGGA ATCCAGTGGT GCAAAAAGCC CAATTGCACC TTCTGTGGTA
    ACAGCTTCTA AACCAAATAA TATGGGAAAG GAAACTACAA GTCAAGCTGA GCGGCCTTCA
    CAGGCAACCT CTGCAGACAC AGCACAGCCT AAGACTGAAA AGACATCCAC AGCAGGTGGT
    GCAGGTGCAG CAGGTGCAGG TGCTGTCGCG AGTATGGTTG CTGCAGCTGA TAAACCAAAT
    AGTGAGCCTG CTATGGATGA ATCAGCACTG GACAGCTTAA TAGATACGCT AGGTGGATCT
    GAAGAAGATG TGGCTACTAG ACCAGTTTAC ACTGGTCCGG AGATTACGGA AAATATTTCT
    TCAGAATACT TGGAAGAGCT GGGTAAACGA GAAGGTAGCC TCCCTCCAGA GTACCTTGCA
    TTATTGAAGA GCAAAGTGGA TGGCAAAGAT GGAGGCCTAC CAAAAGTAGA TGAACATTCT
    GAAAAACCGA TGACGGATGA TGAGCTTGCA GATGCCCTGT CATCTGACTT TACCTGTAGC
    ATTGCTGCTG CTGAAGAAAA GAAGACAACA GAGAAACAAA CTAAGGAAGG AGAAATCATA
    CAAGCACAAG TGACAAGTTC AGTGAAGACT TCAGTTCCTC CTAAAGAGAA GAAAACAAAA
    TCAAAAGAGG AAATCAAAGA AGATGCAATG GAAGCTCTCA TGGCTACTAT TGGTGGACCT
    GAACCTGAAC CCGAACCAAA AGATGTTAGC CCTATTGTAG AAGTGTCAGA GGCAAAAGCT
    AAAGAGAAAA AAGAAAAGAA GGCTGGAGAA CGTGATGATA CAATACCTCC AGAATACAGG
    CTAACTCCAG AGCTGGATAA AGATGGAAAA CCAGTATTGC CAAAGCCTGA AGAAAAACCT
    AAGCCTTTGA GTGAATCTGA CCTTGTTGAT GAATTTTCAA AAGACTTTGC CAGCCCTGCA
    CAGCCTGCAG TACAATCCAA ACCATCAAAG CCCAGCAACA CATCCAAAAA GCCTTCAGCT
    CCTGCATCTA CAAAAACTGC AAAGGATGAA GTAGTCCCTC GTGCCACAGC TTGCTCTGTG
    CAGTCTTCAG CTCCAACATC TGTGTCTTCT GTTGGTCACG TAGCTGATGA AGCAGTAGAA
    GCCTTGTCCA GTAGTCTGGG AGAAAGGGAG CCTGATCCAG AAGAAAAGAA ACCTGCTGTG
    GACAAAGTTA AGGAGAAAGC CAAACGGAAA CAACACAAAA AACTGGGTGA AGATGAAGAA
    ACAATTCCTC CAGAGTACAG ATTAACAGAT GCAAAAGATA AAGATGGAAG ACCACTGTTA
    ATAAAACCTG AAGAGGAGTC CCAGCCTATG AGTGAAAACG ATCTGTTAGA AGGTTTGACA
    AAGGGATTTT CTCCAGCCCA ATCTGCACCA TTACCTACAC AAACTGTAAA AAAGAAAACC
    AAAGGGGGTA AAAAAACTGC AGATTCTTCA GATGTTATCT CTGCTTCTAC AATTTCCTCA
    GTGCACTCAG CAGCTCCCCT AGCTTCTACG TCGGGAGGAA AAGAGATGGA TGATGCCTTG
    GATCTCTTGT CTGATTCCCT GGGACAGAGA GAACCTGACC CTGATGAGAA CAAACCAGTT
    GTGGATAAAG TAAAGGAAAA AGCTAAGTCT GAACACAGAG ACAAACTTGG AGAAAGAGAT
    GATACTATTC CACCTGACTA TCGAAAACTC CTGGAGTCAT CTGATGAGGG TAAACCTGTC
    AAGCCAACAC CAAAGGATGC TTCGAAAAAC AAAGAACAAA AGAAGCCTAC AGATGAGTCT
    GCTGCTATTG ATGCCCTGTC AGGCGACTTT GATACTAGTC CAAAGGCTCC TGCCACACCA
    CAGCACTCAA AGTGTAGGAG AAAAGTGGAA AGGAAACCGT CACCACTAAA GCAACCCCAA
    AGGATGAAAG AAAACCCAAG GATCATAAAA AGGCAAGGGG ACAGTCCTCC AGCAGCAAAT
    CTGAGAAGCA GAAAACAAGC TAAACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48197
    Clone ID Related Accession (Same CDS sequence) XM_015280527.1
    Accession Version XM_015280527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGCAGTCTCC CTCTGCGGAT
    GCACCAAAAC CACCCATTAT GAAGCCATCT GAAGCAAAGT CCACACCAAC AATCCAAACA
    GAACCTGAGA AAACTGCGCT GTCAACAAAG CCATCAAACT CTCAAAATAA TCAACTGGCT
    GAAGAGAAGC CAAAGGAATC CAGTGGTGCA AAAAGCCCAA TTGCACCTTC TGTGGTAACA
    GCTTCTAAAC CAAATAATAT GGGAAAGGAA ACTACAAGTC AAGCTGAGCG GCCTTCACAG
    GCAACCTCTG CAGACACAGC ACAGCCTAAG ACTGAAAAGA CATCCACAGC AGGTGGTGCA
    GGTGCAGCAG GTGCAGGTGC TGTCGCGAGT ATGGTTGCTG CAGCTGATAA ACCAAATAGT
    GAGCCTGCTA TGGATGAATC AGCACTGGAC AGCTTAATAG ATACGCTAGG TGGATCTGAA
    GAAGATGTGG CTACTAGACC AGTTTACACT GGTCCGGAGA TTACGGAAAA TATTTCTTCA
    GAATACTTGG AAGAGCTGGG TAAACGAGAA GGTAGCCTCC CTCCAGAGTA CCTTGCATTA
    TTGAAGAGCA AAGTGGATGG CAAAGATGGA GGCCTACCAA AAGTAGATGA ACATTCTGAA
    AAACCGATGA CGGATGATGA GCTTGCAGAT GCCCTGTCAT CTGACTTTAC CTGTAGCATT
    GCTGCTGCTG AAGAAAAGAA GACAACAGAG AAACAAACTA AGGAAGGAGA AATCATACAA
    GCACAAGTGA CAAGTTCAGT GAAGACTTCA GTTCCTCCTA AAGAGAAGAA AACAAAATCA
    AAAGAGGAAA TCAAAGAAGA TGCAATGGAA GCTCTCATGG CTACTATTGG TGGACCTGAA
    CCTGAACCCG AACCAAAAGA TGTTAGCCCT ATTGTAGAAG TGTCAGAGGC AAAAGCTAAA
    GAGAAAAAAG AAAAGAAGGC TGGAGAACGT GATGATACAA TACCTCCAGA ATACAGGCTA
    ACTCCAGAGC TGGATAAAGA TGGAAAACCA GTATTGCCAA AGCCTGAAGA AAAACCTAAG
    CCTTTGAGTG AATCTGACCT TGTTGATGAA TTTTCAAAAG ACTTTGCCAG CCCTGCACAG
    CCTGCAGTAC AATCCAAACC ATCAAAGCCC AGCAACACAT CCAAAAAGCC TTCAGCTCCT
    GCATCTACAA AAACTGCAAA GGATGAAGTA GTCCCTCGTG CCACAGCTTG CTCTGTGCAG
    TCTTCAGCTC CAACATCTGT GTCTTCTGTT GGTCACGTAG CTGATGAAGC AGTAGAAGCC
    TTGTCCAGTA GTCTGGGAGA AAGGGAGCCT GATCCAGAAG AAAAGAAACC TGCTGTGGAC
    AAAGTTAAGG AGAAAGCCAA ACGGAAACAA CACAAAAAAC TGGGTGAAGA TGAAGAAACA
    ATTCCTCCAG AGTACAGATT AACAGATGCA AAAGATAAAG ATGGAAGACC ACTGTTAATA
    AAACCTGAAG AGGAGTCCCA GCCTATGAGT GAAAACGATC TGTTAGAAGG TTTGACAAAG
    GGATTTTCTC CAGCCCAATC TGCACCATTA CCTACACAAA CTGTAAAAAA GAAAACCAAA
    GGGGGTAAAA AAACTGCAGA TTCTTCAGAT GTTATCTCTG CTTCTACAAT TTCCTCAGTG
    CACTCAGCAG CTCCCCTAGC TTCTACGTCG GGAGGAAAAG AGATGGATGA TGCCTTGGAT
    CTCTTGTCTG ATTCCCTGGG ACAGAGAGAA CCTGACCCTG ATGAGAACAA ACCAGTTGTG
    GATAAAGTAA AGGAAAAAGC TAAGTCTGAA CACAGAGACA AACTTGGAGA AAGAGATGAT
    ACTATTCCAC CTGACTATCG AAAACTCCTG GAGTCATCTG ATGAGGGTAA ACCTGTCAAG
    CCAACACCAA AGGATGCTTC GAAAAACAAA GAACAAAAGA AGCCTACAGA TGAGTCTGCT
    GCTATTGATG CCCTGTCAGG CGACTTTGAT ACTAGTCCAA AGGCTCCTGC CACACCACAG
    CACTCAAAGT GTAGGAGAAA AGTGGAAAGG AAACCGTCAC CACTAAAGCA ACCCCAAAGG
    ATGAAAGAAA ACCCAAGGAT CATAAAAAGG CAAGGGGACA GTCCTCCAGC AGCAAATCTG
    AGAAGCAGAA AACAAGCTAA ACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136013.1
    CDS95..2341
    Translation

    Target ORF information:

    RefSeq Version XM_015280527.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGCAGTCTCC CTCTGCGGAT
    GCACCAAAAC CACCCATTAT GAAGCCATCT GAAGCAAAGT CCACACCAAC AATCCAAACA
    GAACCTGAGA AAACTGCGCT GTCAACAAAG CCATCAAACT CTCAAAATAA TCAACTGGCT
    GAAGAGAAGC CAAAGGAATC CAGTGGTGCA AAAAGCCCAA TTGCACCTTC TGTGGTAACA
    GCTTCTAAAC CAAATAATAT GGGAAAGGAA ACTACAAGTC AAGCTGAGCG GCCTTCACAG
    GCAACCTCTG CAGACACAGC ACAGCCTAAG ACTGAAAAGA CATCCACAGC AGGTGGTGCA
    GGTGCAGCAG GTGCAGGTGC TGTCGCGAGT ATGGTTGCTG CAGCTGATAA ACCAAATAGT
    GAGCCTGCTA TGGATGAATC AGCACTGGAC AGCTTAATAG ATACGCTAGG TGGATCTGAA
    GAAGATGTGG CTACTAGACC AGTTTACACT GGTCCGGAGA TTACGGAAAA TATTTCTTCA
    GAATACTTGG AAGAGCTGGG TAAACGAGAA GGTAGCCTCC CTCCAGAGTA CCTTGCATTA
    TTGAAGAGCA AAGTGGATGG CAAAGATGGA GGCCTACCAA AAGTAGATGA ACATTCTGAA
    AAACCGATGA CGGATGATGA GCTTGCAGAT GCCCTGTCAT CTGACTTTAC CTGTAGCATT
    GCTGCTGCTG AAGAAAAGAA GACAACAGAG AAACAAACTA AGGAAGGAGA AATCATACAA
    GCACAAGTGA CAAGTTCAGT GAAGACTTCA GTTCCTCCTA AAGAGAAGAA AACAAAATCA
    AAAGAGGAAA TCAAAGAAGA TGCAATGGAA GCTCTCATGG CTACTATTGG TGGACCTGAA
    CCTGAACCCG AACCAAAAGA TGTTAGCCCT ATTGTAGAAG TGTCAGAGGC AAAAGCTAAA
    GAGAAAAAAG AAAAGAAGGC TGGAGAACGT GATGATACAA TACCTCCAGA ATACAGGCTA
    ACTCCAGAGC TGGATAAAGA TGGAAAACCA GTATTGCCAA AGCCTGAAGA AAAACCTAAG
    CCTTTGAGTG AATCTGACCT TGTTGATGAA TTTTCAAAAG ACTTTGCCAG CCCTGCACAG
    CCTGCAGTAC AATCCAAACC ATCAAAGCCC AGCAACACAT CCAAAAAGCC TTCAGCTCCT
    GCATCTACAA AAACTGCAAA GGATGAAGTA GTCCCTCGTG CCACAGCTTG CTCTGTGCAG
    TCTTCAGCTC CAACATCTGT GTCTTCTGTT GGTCACGTAG CTGATGAAGC AGTAGAAGCC
    TTGTCCAGTA GTCTGGGAGA AAGGGAGCCT GATCCAGAAG AAAAGAAACC TGCTGTGGAC
    AAAGTTAAGG AGAAAGCCAA ACGGAAACAA CACAAAAAAC TGGGTGAAGA TGAAGAAACA
    ATTCCTCCAG AGTACAGATT AACAGATGCA AAAGATAAAG ATGGAAGACC ACTGTTAATA
    AAACCTGAAG AGGAGTCCCA GCCTATGAGT GAAAACGATC TGTTAGAAGG TTTGACAAAG
    GGATTTTCTC CAGCCCAATC TGCACCATTA CCTACACAAA CTGTAAAAAA GAAAACCAAA
    GGGGGTAAAA AAACTGCAGA TTCTTCAGAT GTTATCTCTG CTTCTACAAT TTCCTCAGTG
    CACTCAGCAG CTCCCCTAGC TTCTACGTCG GGAGGAAAAG AGATGGATGA TGCCTTGGAT
    CTCTTGTCTG ATTCCCTGGG ACAGAGAGAA CCTGACCCTG ATGAGAACAA ACCAGTTGTG
    GATAAAGTAA AGGAAAAAGC TAAGTCTGAA CACAGAGACA AACTTGGAGA AAGAGATGAT
    ACTATTCCAC CTGACTATCG AAAACTCCTG GAGTCATCTG ATGAGGGTAA ACCTGTCAAG
    CCAACACCAA AGGATGCTTC GAAAAACAAA GAACAAAAGA AGCCTACAGA TGAGTCTGCT
    GCTATTGATG CCCTGTCAGG CGACTTTGAT ACTAGTCCAA AGGCTCCTGC CACACCACAG
    CACTCAAAGT GTAGGAGAAA AGTGGAAAGG AAACCGTCAC CACTAAAGCA ACCCCAAAGG
    ATGAAAGAAA ACCCAAGGAT CATAAAAAGG CAAGGGGACA GTCCTCCAGC AGCAAATCTG
    AGAAGCAGAA AACAAGCTAA ACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48198
    Clone ID Related Accession (Same CDS sequence) XM_015280528.1
    Accession Version XM_015280528.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT
    CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA
    ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT
    AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT
    TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG
    CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA
    GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT
    AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA
    GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA
    AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG
    TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT
    GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT
    ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA
    GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG
    AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT
    GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG
    GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA
    GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA
    GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC
    AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG
    CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT
    TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA
    GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA
    CCTGCTGTGG ACAAAGTTAA GGATAAAGAT GGAAGACCAC TGTTAATAAA ACCTGAAGAG
    GAGTCCCAGC CTATGAGTGA AAACGATCTG TTAGAAGGTT TGACAAAGGG ATTTTCTCCA
    GCCCAATCTG CACCATTACC TACACAAACT GTAAAAAAGA AAACCAAAGG GGGTAAAAAA
    ACTGCAGATT CTTCAGATGT TATCTCTGCT TCTACAATTT CCTCAGTGCA CTCAGCAGCT
    CCCCTAGCTT CTACGTCGGG AGGAAAAGAG ATGGATGATG CCTTGGATCT CTTGTCTGAT
    TCCCTGGGAC AGAGAGAACC TGACCCTGAT GAGAACAAAC CAGTTGTGGA TAAAGTAAAG
    GAAAAAGCTA AGTCTGAACA CAGAGACAAA CTTGGAGAAA GAGATGATAC TATTCCACCT
    GACTATCGAA AACTCCTGGA GTCATCTGAT GAGGGTAAAC CTGTCAAGCC AACACCAAAG
    GATGCTTCGA AAAACAAAGA ACAAAAGAAG CCTACAGATG AGTCTGCTGC TATTGATGCC
    CTGTCAGGCG ACTTTGATAC TAGTCCAAAG GCTCCTGCCA CACCACAGCA CTCAAAGTGT
    AGGAGAAAAG TGGAAAGGAA ACCGTCACCA CTAAAGCAAC CCCAAAGGAT GAAAGAAAAC
    CCAAGGATCA TAAAAAGGCA AGGGGACAGT CCTCCAGCAG CAAATCTGAG AAGCAGAAAA
    CAAGCTAAAC GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136014.1
    CDS95..2329
    Translation

    Target ORF information:

    RefSeq Version XM_015280528.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
    GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT
    GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT
    CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA
    ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT
    AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT
    TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG
    CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA
    GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT
    AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA
    GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA
    AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG
    TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT
    GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT
    ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA
    GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG
    AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT
    GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG
    GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA
    GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA
    GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC
    AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG
    CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT
    TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA
    GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA
    CCTGCTGTGG ACAAAGTTAA GGATAAAGAT GGAAGACCAC TGTTAATAAA ACCTGAAGAG
    GAGTCCCAGC CTATGAGTGA AAACGATCTG TTAGAAGGTT TGACAAAGGG ATTTTCTCCA
    GCCCAATCTG CACCATTACC TACACAAACT GTAAAAAAGA AAACCAAAGG GGGTAAAAAA
    ACTGCAGATT CTTCAGATGT TATCTCTGCT TCTACAATTT CCTCAGTGCA CTCAGCAGCT
    CCCCTAGCTT CTACGTCGGG AGGAAAAGAG ATGGATGATG CCTTGGATCT CTTGTCTGAT
    TCCCTGGGAC AGAGAGAACC TGACCCTGAT GAGAACAAAC CAGTTGTGGA TAAAGTAAAG
    GAAAAAGCTA AGTCTGAACA CAGAGACAAA CTTGGAGAAA GAGATGATAC TATTCCACCT
    GACTATCGAA AACTCCTGGA GTCATCTGAT GAGGGTAAAC CTGTCAAGCC AACACCAAAG
    GATGCTTCGA AAAACAAAGA ACAAAAGAAG CCTACAGATG AGTCTGCTGC TATTGATGCC
    CTGTCAGGCG ACTTTGATAC TAGTCCAAAG GCTCCTGCCA CACCACAGCA CTCAAAGTGT
    AGGAGAAAAG TGGAAAGGAA ACCGTCACCA CTAAAGCAAC CCCAAAGGAT GAAAGAAAAC
    CCAAGGATCA TAAAAAGGCA AGGGGACAGT CCTCCAGCAG CAAATCTGAG AAGCAGAAAA
    CAAGCTAAAC GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48199
    Clone ID Related Accession (Same CDS sequence) XM_015280529.1 , XM_015280529.2
    Accession Version XM_015280529.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAGCCAT CTGAAGCAAA GTCCACACCA ACAATCCAAA CAGAACCTGA GAAAACTGCG 
    CTGTCAACAA AGCCATCAAA CTCTCAAAAT AATCAACTGG CTGAAGAGAA GCCAAAGGAA
    TCCAGTGGTG CAAAAAGCCC AATTGCACCT TCTGTGGTAA CAGCTTCTAA ACCAAATAAT
    ATGGGAAAGG AAACTACAAG TCAAGCTGAG CGGCCTTCAC AGGCAACCTC TGCAGACACA
    GCACAGCCTA AGACTGAAAA GACATCCACA GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT
    GCTGTCGCGA GTATGGTTGC TGCAGCTGAT AAACCAAATA GTGAGCCTGC TATGGATGAA
    TCAGCACTGG ACAGCTTAAT AGATACGCTA GGTGGATCTG AAGAAGATGT GGCTACTAGA
    CCAGTTTACA CTGGTCCGGA GATTACGGAA AATATTTCTT CAGAATACTT GGAAGAGCTG
    GGTAAACGAG AAGGTAGCCT CCCTCCAGAG TACCTTGCAT TATTGAAGAG CAAAGTGGAT
    GGCAAAGATG GAGGCCTACC AAAAGTAGAT GAACATTCTG AAAAACCGAT GACGGATGAT
    GAGCTTGCAG ATGCCCTGTC ATCTGACTTT ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG
    AAGACAACAG AGAAACAAAC TAAGGAAGGA GAAATCATAC AAGCACAAGT GACAAGTTCA
    GTGAAGACTT CAGTTCCTCC TAAAGAGAAG AAAACAAAAT CAAAAGAGGA AATCAAAGAA
    GATGCAATGG AAGCTCTCAT GGCTACTATT GGTGGACCTG AACCTGAACC CGAACCAAAA
    GATGTTAGCC CTATTGTAGA AGTGTCAGAG GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG
    GCTGGAGAAC GTGATGATAC AATACCTCCA GAATACAGGC TAACTCCAGA GCTGGATAAA
    GATGGAAAAC CAGTATTGCC AAAGCCTGAA GAAAAACCTA AGCCTTTGAG TGAATCTGAC
    CTTGTTGATG AATTTTCAAA AGACTTTGCC AGCCCTGCAC AGCCTGCAGT ACAATCCAAA
    CCATCAAAGC CCAGCAACAC ATCCAAAAAG CCTTCAGCTC CTGCATCTAC AAAAACTGCA
    AAGGATGAAG TAGTCCCTCG TGCCACAGCT TGCTCTGTGC AGTCTTCAGC TCCAACATCT
    GTGTCTTCTG TTGGTCACGT AGCTGATGAA GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA
    GAAAGGGAGC CTGATCCAGA AGAAAAGAAA CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC
    AAACGGAAAC AACACAAAAA ACTGGGTGAA GATGAAGAAA CAATTCCTCC AGAGTACAGA
    TTAACAGATG CAAAAGATAA AGATGGAAGA CCACTGTTAA TAAAACCTGA AGAGGAGTCC
    CAGCCTATGA GTGAAAACGA TCTGTTAGAA GGTTTGACAA AGGGATTTTC TCCAGCCCAA
    TCTGCACCAT TACCTACACA AACTGTAAAA AAGAAAACCA AAGGGGGTAA AAAAACTGCA
    GATTCTTCAG ATGTTATCTC TGCTTCTACA ATTTCCTCAG TGCACTCAGC AGCTCCCCTA
    GCTTCTACGT CGGGAGGAAA AGAGATGGAT GATGCCTTGG ATCTCTTGTC TGATTCCCTG
    GGACAGAGAG AACCTGACCC TGATGAGAAC AAACCAGTTG TGGATAAAGT AAAGGAAAAA
    GCTAAGTCTG AACACAGAGA CAAACTTGGA GAAAGAGATG ATACTATTCC ACCTGACTAT
    CGAAAACTCC TGGAGTCATC TGATGAGGGT AAACCTGTCA AGCCAACACC AAAGGATGCT
    TCGAAAAACA AAGAACAAAA GAAGCCTACA GATGAGTCTG CTGCTATTGA TGCCCTGTCA
    GGCGACTTTG ATACTAGTCC AAAGGCTCCT GCCACACCAC AGCACTCAAA GTGTAGGAGA
    AAAGTGGAAA GGAAACCGTC ACCACTAAAG CAACCCCAAA GGATGAAAGA AAACCCAAGG
    ATCATAAAAA GGCAAGGGGA CAGTCCTCCA GCAGCAAATC TGAGAAGCAG AAAACAAGCT
    AAACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136015.1
    CDS147..2255
    Translation

    Target ORF information:

    RefSeq Version XM_015280529.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAGCCAT CTGAAGCAAA GTCCACACCA ACAATCCAAA CAGAACCTGA GAAAACTGCG 
    CTGTCAACAA AGCCATCAAA CTCTCAAAAT AATCAACTGG CTGAAGAGAA GCCAAAGGAA
    TCCAGTGGTG CAAAAAGCCC AATTGCACCT TCTGTGGTAA CAGCTTCTAA ACCAAATAAT
    ATGGGAAAGG AAACTACAAG TCAAGCTGAG CGGCCTTCAC AGGCAACCTC TGCAGACACA
    GCACAGCCTA AGACTGAAAA GACATCCACA GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT
    GCTGTCGCGA GTATGGTTGC TGCAGCTGAT AAACCAAATA GTGAGCCTGC TATGGATGAA
    TCAGCACTGG ACAGCTTAAT AGATACGCTA GGTGGATCTG AAGAAGATGT GGCTACTAGA
    CCAGTTTACA CTGGTCCGGA GATTACGGAA AATATTTCTT CAGAATACTT GGAAGAGCTG
    GGTAAACGAG AAGGTAGCCT CCCTCCAGAG TACCTTGCAT TATTGAAGAG CAAAGTGGAT
    GGCAAAGATG GAGGCCTACC AAAAGTAGAT GAACATTCTG AAAAACCGAT GACGGATGAT
    GAGCTTGCAG ATGCCCTGTC ATCTGACTTT ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG
    AAGACAACAG AGAAACAAAC TAAGGAAGGA GAAATCATAC AAGCACAAGT GACAAGTTCA
    GTGAAGACTT CAGTTCCTCC TAAAGAGAAG AAAACAAAAT CAAAAGAGGA AATCAAAGAA
    GATGCAATGG AAGCTCTCAT GGCTACTATT GGTGGACCTG AACCTGAACC CGAACCAAAA
    GATGTTAGCC CTATTGTAGA AGTGTCAGAG GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG
    GCTGGAGAAC GTGATGATAC AATACCTCCA GAATACAGGC TAACTCCAGA GCTGGATAAA
    GATGGAAAAC CAGTATTGCC AAAGCCTGAA GAAAAACCTA AGCCTTTGAG TGAATCTGAC
    CTTGTTGATG AATTTTCAAA AGACTTTGCC AGCCCTGCAC AGCCTGCAGT ACAATCCAAA
    CCATCAAAGC CCAGCAACAC ATCCAAAAAG CCTTCAGCTC CTGCATCTAC AAAAACTGCA
    AAGGATGAAG TAGTCCCTCG TGCCACAGCT TGCTCTGTGC AGTCTTCAGC TCCAACATCT
    GTGTCTTCTG TTGGTCACGT AGCTGATGAA GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA
    GAAAGGGAGC CTGATCCAGA AGAAAAGAAA CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC
    AAACGGAAAC AACACAAAAA ACTGGGTGAA GATGAAGAAA CAATTCCTCC AGAGTACAGA
    TTAACAGATG CAAAAGATAA AGATGGAAGA CCACTGTTAA TAAAACCTGA AGAGGAGTCC
    CAGCCTATGA GTGAAAACGA TCTGTTAGAA GGTTTGACAA AGGGATTTTC TCCAGCCCAA
    TCTGCACCAT TACCTACACA AACTGTAAAA AAGAAAACCA AAGGGGGTAA AAAAACTGCA
    GATTCTTCAG ATGTTATCTC TGCTTCTACA ATTTCCTCAG TGCACTCAGC AGCTCCCCTA
    GCTTCTACGT CGGGAGGAAA AGAGATGGAT GATGCCTTGG ATCTCTTGTC TGATTCCCTG
    GGACAGAGAG AACCTGACCC TGATGAGAAC AAACCAGTTG TGGATAAAGT AAAGGAAAAA
    GCTAAGTCTG AACACAGAGA CAAACTTGGA GAAAGAGATG ATACTATTCC ACCTGACTAT
    CGAAAACTCC TGGAGTCATC TGATGAGGGT AAACCTGTCA AGCCAACACC AAAGGATGCT
    TCGAAAAACA AAGAACAAAA GAAGCCTACA GATGAGTCTG CTGCTATTGA TGCCCTGTCA
    GGCGACTTTG ATACTAGTCC AAAGGCTCCT GCCACACCAC AGCACTCAAA GTGTAGGAGA
    AAAGTGGAAA GGAAACCGTC ACCACTAAAG CAACCCCAAA GGATGAAAGA AAACCCAAGG
    ATCATAAAAA GGCAAGGGGA CAGTCCTCCA GCAGCAAATC TGAGAAGCAG AAAACAAGCT
    AAACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48199
    Clone ID Related Accession (Same CDS sequence) XM_015280529.1 , XM_015280529.2
    Accession Version XM_015280529.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product calpastatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAGCCAT CTGAAGCAAA GTCCACACCA ACAATCCAAA CAGAACCTGA GAAAACTGCG 
    CTGTCAACAA AGCCATCAAA CTCTCAAAAT AATCAACTGG CTGAAGAGAA GCCAAAGGAA
    TCCAGTGGTG CAAAAAGCCC AATTGCACCT TCTGTGGTAA CAGCTTCTAA ACCAAATAAT
    ATGGGAAAGG AAACTACAAG TCAAGCTGAG CGGCCTTCAC AGGCAACCTC TGCAGACACA
    GCACAGCCTA AGACTGAAAA GACATCCACA GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT
    GCTGTCGCGA GTATGGTTGC TGCAGCTGAT AAACCAAATA GTGAGCCTGC TATGGATGAA
    TCAGCACTGG ACAGCTTAAT AGATACGCTA GGTGGATCTG AAGAAGATGT GGCTACTAGA
    CCAGTTTACA CTGGTCCGGA GATTACGGAA AATATTTCTT CAGAATACTT GGAAGAGCTG
    GGTAAACGAG AAGGTAGCCT CCCTCCAGAG TACCTTGCAT TATTGAAGAG CAAAGTGGAT
    GGCAAAGATG GAGGCCTACC AAAAGTAGAT GAACATTCTG AAAAACCGAT GACGGATGAT
    GAGCTTGCAG ATGCCCTGTC ATCTGACTTT ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG
    AAGACAACAG AGAAACAAAC TAAGGAAGGA GAAATCATAC AAGCACAAGT GACAAGTTCA
    GTGAAGACTT CAGTTCCTCC TAAAGAGAAG AAAACAAAAT CAAAAGAGGA AATCAAAGAA
    GATGCAATGG AAGCTCTCAT GGCTACTATT GGTGGACCTG AACCTGAACC CGAACCAAAA
    GATGTTAGCC CTATTGTAGA AGTGTCAGAG GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG
    GCTGGAGAAC GTGATGATAC AATACCTCCA GAATACAGGC TAACTCCAGA GCTGGATAAA
    GATGGAAAAC CAGTATTGCC AAAGCCTGAA GAAAAACCTA AGCCTTTGAG TGAATCTGAC
    CTTGTTGATG AATTTTCAAA AGACTTTGCC AGCCCTGCAC AGCCTGCAGT ACAATCCAAA
    CCATCAAAGC CCAGCAACAC ATCCAAAAAG CCTTCAGCTC CTGCATCTAC AAAAACTGCA
    AAGGATGAAG TAGTCCCTCG TGCCACAGCT TGCTCTGTGC AGTCTTCAGC TCCAACATCT
    GTGTCTTCTG TTGGTCACGT AGCTGATGAA GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA
    GAAAGGGAGC CTGATCCAGA AGAAAAGAAA CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC
    AAACGGAAAC AACACAAAAA ACTGGGTGAA GATGAAGAAA CAATTCCTCC AGAGTACAGA
    TTAACAGATG CAAAAGATAA AGATGGAAGA CCACTGTTAA TAAAACCTGA AGAGGAGTCC
    CAGCCTATGA GTGAAAACGA TCTGTTAGAA GGTTTGACAA AGGGATTTTC TCCAGCCCAA
    TCTGCACCAT TACCTACACA AACTGTAAAA AAGAAAACCA AAGGGGGTAA AAAAACTGCA
    GATTCTTCAG ATGTTATCTC TGCTTCTACA ATTTCCTCAG TGCACTCAGC AGCTCCCCTA
    GCTTCTACGT CGGGAGGAAA AGAGATGGAT GATGCCTTGG ATCTCTTGTC TGATTCCCTG
    GGACAGAGAG AACCTGACCC TGATGAGAAC AAACCAGTTG TGGATAAAGT AAAGGAAAAA
    GCTAAGTCTG AACACAGAGA CAAACTTGGA GAAAGAGATG ATACTATTCC ACCTGACTAT
    CGAAAACTCC TGGAGTCATC TGATGAGGGT AAACCTGTCA AGCCAACACC AAAGGATGCT
    TCGAAAAACA AAGAACAAAA GAAGCCTACA GATGAGTCTG CTGCTATTGA TGCCCTGTCA
    GGCGACTTTG ATACTAGTCC AAAGGCTCCT GCCACACCAC AGCACTCAAA GTGTAGGAGA
    AAAGTGGAAA GGAAACCGTC ACCACTAAAG CAACCCCAAA GGATGAAAGA AAACCCAAGG
    ATCATAAAAA GGCAAGGGGA CAGTCCTCCA GCAGCAAATC TGAGAAGCAG AAAACAAGCT
    AAACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136015.1
    CDS165..2273
    Translation

    Target ORF information:

    RefSeq Version XM_015280529.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calpastatin (CAST), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280529.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAGCCAT CTGAAGCAAA GTCCACACCA ACAATCCAAA CAGAACCTGA GAAAACTGCG 
    CTGTCAACAA AGCCATCAAA CTCTCAAAAT AATCAACTGG CTGAAGAGAA GCCAAAGGAA
    TCCAGTGGTG CAAAAAGCCC AATTGCACCT TCTGTGGTAA CAGCTTCTAA ACCAAATAAT
    ATGGGAAAGG AAACTACAAG TCAAGCTGAG CGGCCTTCAC AGGCAACCTC TGCAGACACA
    GCACAGCCTA AGACTGAAAA GACATCCACA GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT
    GCTGTCGCGA GTATGGTTGC TGCAGCTGAT AAACCAAATA GTGAGCCTGC TATGGATGAA
    TCAGCACTGG ACAGCTTAAT AGATACGCTA GGTGGATCTG AAGAAGATGT GGCTACTAGA
    CCAGTTTACA CTGGTCCGGA GATTACGGAA AATATTTCTT CAGAATACTT GGAAGAGCTG
    GGTAAACGAG AAGGTAGCCT CCCTCCAGAG TACCTTGCAT TATTGAAGAG CAAAGTGGAT
    GGCAAAGATG GAGGCCTACC AAAAGTAGAT GAACATTCTG AAAAACCGAT GACGGATGAT
    GAGCTTGCAG ATGCCCTGTC ATCTGACTTT ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG
    AAGACAACAG AGAAACAAAC TAAGGAAGGA GAAATCATAC AAGCACAAGT GACAAGTTCA
    GTGAAGACTT CAGTTCCTCC TAAAGAGAAG AAAACAAAAT CAAAAGAGGA AATCAAAGAA
    GATGCAATGG AAGCTCTCAT GGCTACTATT GGTGGACCTG AACCTGAACC CGAACCAAAA
    GATGTTAGCC CTATTGTAGA AGTGTCAGAG GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG
    GCTGGAGAAC GTGATGATAC AATACCTCCA GAATACAGGC TAACTCCAGA GCTGGATAAA
    GATGGAAAAC CAGTATTGCC AAAGCCTGAA GAAAAACCTA AGCCTTTGAG TGAATCTGAC
    CTTGTTGATG AATTTTCAAA AGACTTTGCC AGCCCTGCAC AGCCTGCAGT ACAATCCAAA
    CCATCAAAGC CCAGCAACAC ATCCAAAAAG CCTTCAGCTC CTGCATCTAC AAAAACTGCA
    AAGGATGAAG TAGTCCCTCG TGCCACAGCT TGCTCTGTGC AGTCTTCAGC TCCAACATCT
    GTGTCTTCTG TTGGTCACGT AGCTGATGAA GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA
    GAAAGGGAGC CTGATCCAGA AGAAAAGAAA CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC
    AAACGGAAAC AACACAAAAA ACTGGGTGAA GATGAAGAAA CAATTCCTCC AGAGTACAGA
    TTAACAGATG CAAAAGATAA AGATGGAAGA CCACTGTTAA TAAAACCTGA AGAGGAGTCC
    CAGCCTATGA GTGAAAACGA TCTGTTAGAA GGTTTGACAA AGGGATTTTC TCCAGCCCAA
    TCTGCACCAT TACCTACACA AACTGTAAAA AAGAAAACCA AAGGGGGTAA AAAAACTGCA
    GATTCTTCAG ATGTTATCTC TGCTTCTACA ATTTCCTCAG TGCACTCAGC AGCTCCCCTA
    GCTTCTACGT CGGGAGGAAA AGAGATGGAT GATGCCTTGG ATCTCTTGTC TGATTCCCTG
    GGACAGAGAG AACCTGACCC TGATGAGAAC AAACCAGTTG TGGATAAAGT AAAGGAAAAA
    GCTAAGTCTG AACACAGAGA CAAACTTGGA GAAAGAGATG ATACTATTCC ACCTGACTAT
    CGAAAACTCC TGGAGTCATC TGATGAGGGT AAACCTGTCA AGCCAACACC AAAGGATGCT
    TCGAAAAACA AAGAACAAAA GAAGCCTACA GATGAGTCTG CTGCTATTGA TGCCCTGTCA
    GGCGACTTTG ATACTAGTCC AAAGGCTCCT GCCACACCAC AGCACTCAAA GTGTAGGAGA
    AAAGTGGAAA GGAAACCGTC ACCACTAAAG CAACCCCAAA GGATGAAAGA AAACCCAAGG
    ATCATAAAAA GGCAAGGGGA CAGTCCTCCA GCAGCAAATC TGAGAAGCAG AAAACAAGCT
    AAACGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.