CAST gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015280528.1	XP_015136014.1	calpastatin isoform X6
XM_015280524.1	XP_015136010.1	calpastatin isoform X2
XM_015280523.1	XP_015136009.1	calpastatin isoform X1
XM_015280526.1	XP_015136012.1	calpastatin isoform X4
XM_015280527.1	XP_015136013.1	calpastatin isoform X5
XM_025144706.1	XP_025000474.1	calpastatin isoform X1
XM_015280525.1	XP_015136011.1	calpastatin isoform X3
XM_015280529.1	XP_015136015.1	calpastatin isoform X7
XM_015280529.2	XP_015136015.1	calpastatin isoform X2
NM_001137650.1	NP_001131122.1	calpastatin

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa00493	NM_001137650.1 Latest version!	Gallus gallus calpastatin (CAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OGa152979	XM_025144706.1 Latest version!	Gallus gallus calpastatin (CAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa48193	XM_015280523.1 Latest version!	Gallus gallus calpastatin (CAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa48194	XM_015280524.1 Latest version!	Gallus gallus calpastatin (CAST), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa48195	XM_015280525.1 Latest version!	Gallus gallus calpastatin (CAST), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa48196	XM_015280526.1 Latest version!	Gallus gallus calpastatin (CAST), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa48197	XM_015280527.1 Latest version!	Gallus gallus calpastatin (CAST), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa48198	XM_015280528.1 Latest version!	Gallus gallus calpastatin (CAST), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa48199	XM_015280529.2 Latest version!	Gallus gallus calpastatin (CAST), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OGa48199	XM_015280529.1	Gallus gallus calpastatin (CAST), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

CAST ( NM_001137650.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001131122.1 Gallus gallus calpastatin (CAST), mRNA.

Price & Availability↑

CloneID	OGa00493
Clone ID Related Accession (Same CDS sequence)	NM_001137650.1
Accession Version	NM_001137650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	calpastatin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ232592.1. On Nov 14, 2008 this sequence version replaced XM_424713.2. ##Evidence-Data-START## Transcript exon combination :: FJ232592.1 [ECO:0000332] ##Evidence-Data-END##

ATGAATGGTG TCCCCTATGG GTCCAATGGT GTCCTATGGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT 
CCCTCTGCGG ATGCACCAAA ACCACCCACT ATGAAGCCAT CTGAAGCAAA GTCCACACCA 
ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT 
AATCAACCGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT 
TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG 
CGGCCTTCAC AGGCAACCTC TGCAGACACC GCACAGCCTA AGACTGAAAA GACATCCACA 
GCAGGCGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCACGA GTATGGTTGC TGCAGCTGAT 
AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA 
GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA 
AATATTTCTT CAGAATACTT GGAAGAGCTG GGAAAACGAG AAGGTAGCCT CCCTCCAGAG 
TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT 
GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT 
ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA 
GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG 
AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT 
GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG 
GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA 
GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA 
GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC 
AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG 
CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT 
TGCTCTGTGC AGTCTTCGGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA 
GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA 
CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA 
GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA 
CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA 
GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA 
AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA 
ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT 
GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC 
AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA 
GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT 
AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA 
GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT 
GCCACACCAC AGCACTCAAA GGAGAAAAGT GGAAAGGAAA CCGTCACCAC TAAAGCAACC 
CCAAAGGATG AAAGAAAACC CAAGGATCAT AAAAAGGCAA GGGGACAGTC CTCCAGCAGC 
AAATCTGAGA AGCAGAAAAC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131122.1
CDS	1..2307
Translation	MNGVPYGSNGVLWQGDKKASGTSSKSGEKKEATEKPAGANVSQLPKTQTSGVLPAAKKQSPSADAPKPPTMKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQPAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVTSMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKEKSGKETVTTKATPKDERKPKDHKKARGQSSSSKSEKQKTS

Target ORF information:

RefSeq Version	NM_001137650.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137650.1

ORF Insert Sequence:

ATGAATGGTG TCCCCTATGG GTCCAATGGT GTCCTATGGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT 
CCCTCTGCGG ATGCACCAAA ACCACCCACT ATGAAGCCAT CTGAAGCAAA GTCCACACCA 
ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT 
AATCAACCGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT 
TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG 
CGGCCTTCAC AGGCAACCTC TGCAGACACC GCACAGCCTA AGACTGAAAA GACATCCACA 
GCAGGCGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCACGA GTATGGTTGC TGCAGCTGAT 
AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA 
GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA 
AATATTTCTT CAGAATACTT GGAAGAGCTG GGAAAACGAG AAGGTAGCCT CCCTCCAGAG 
TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT 
GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT 
ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA 
GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG 
AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT 
GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG 
GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA 
GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA 
GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC 
AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG 
CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT 
TGCTCTGTGC AGTCTTCGGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA 
GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA 
CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA 
GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA 
CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA 
GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA 
AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA 
ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT 
GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC 
AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA 
GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT 
AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA 
GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT 
GCCACACCAC AGCACTCAAA GGAGAAAAGT GGAAAGGAAA CCGTCACCAC TAAAGCAACC 
CCAAAGGATG AAAGAAAACC CAAGGATCAT AAAAAGGCAA GGGGACAGTC CTCCAGCAGC 
AAATCTGAGA AGCAGAAAAC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_025144706.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025000474.1 Gallus gallus calpastatin (CAST), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa152979
Clone ID Related Accession (Same CDS sequence)	XM_025144706.1
Accession Version	XM_025144706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCCG ACACCCCCCC CCCCCCGTGC CCGGCCCGCA GTCCCGCCGG CACCGCCCTG 
CTGCGGGGCT CGGTGGCGGC TGGGGCGCGG CCGTGGCCAC TCCCGTGGCT GGTGCCGCCC 
TCAGAGGGAG GTGGCTGCTG GGGCGCGGCC CGTGCGTCTG TGTCCCTTGC CGTGCCGTGC 
CGTGCCGTGC CGTGCGGAGC AGCGAGCAGC GAGCAGCGCC ATGGCTTTCG CGCGCTGGTG 
CAGGGCGACA AGAAGGCAAG TGGAACTTCT TCCAAATCGG GAGAGAAAAA AGAAGCAACA 
GAGAAGCCAG CAGGTGCTAA TGTCAGTCAG CTTCCCAAAA CACAGACAAG TGGAGTACTG 
CCTGCTGCTA AGAAGCAGTC TCCCTCTGCG GATGCACCAA AACCACCCAT TATGAAGCCA 
TCTGAAGCAA AGTCCACACC AACAATCCAA ACAGAACCTG AGAAAACTGC GCTGTCAACA 
AAGCCATCAA ACTCTCAAAA TAATCAACTG GCTGAAGAGA AGCCAAAGGA ATCCAGTGGT 
GCAAAAAGCC CAATTGCACC TTCTGTGGTA ACAGCTTCTA AACCAAATAA TATGGGAAAG 
GAAACTACAA GTCAAGCTGA GCGGCCTTCA CAGGCAACCT CTGCAGACAC AGCACAGCCT 
AAGACTGAAA AGACATCCAC AGCAGGTGGT GCAGGTGCAG CAGGTGCAGG TGCTGTCGCG 
AGTATGGTTG CTGCAGCTGA TAAACCAAAT AGTGAGCCTG CTATGGATGA ATCAGCACTG 
GACAGCTTAA TAGATACGCT AGGTGGATCT GAAGAAGATG TGGCTACTAG ACCAGTTTAC 
ACTGGTCCGG AGATTACGGA AAATATTTCT TCAGAATACT TGGAAGAGCT GGGTAAACGA 
GAAGGTAGCC TCCCTCCAGA GTACCTTGCA TTATTGAAGA GCAAAGTGGA TGGCAAAGAT 
GGAGGCCTAC CAAAAGTAGA TGAACATTCT GAAAAACCGA TGACGGATGA TGAGCTTGCA 
GATGCCCTGT CATCTGACTT TACCTGTAGC ATTGCTGCTG CTGAAGAAAA GAAGACAACA 
GAGAAACAAA CTAAGGAAGG AGAAATCATA CAAGCACAAG TGACAAGTTC AGTGAAGACT 
TCAGTTCCTC CTAAAGAGAA GAAAACAAAA TCAAAAGAGG AAATCAAAGA AGATGCAATG 
GAAGCTCTCA TGGCTACTAT TGGTGGACCT GAACCTGAAC CCGAACCAAA AGATGTTAGC 
CCTATTGTAG AAGTGTCAGA GGCAAAAGCT AAAGAGAAAA AAGAAAAGAA GGCTGGAGAA 
CGTGATGATA CAATACCTCC AGAATACAGG CTAACTCCAG AGCTGGATAA AGATGGAAAA 
CCAGTATTGC CAAAGCCTGA AGAAAAACCT AAGCCTTTGA GTGAATCTGA CCTTGTTGAT 
GAATTTTCAA AAGACTTTGC CAGCCCTGCA CAGCCTGCAG TACAATCCAA ACCATCAAAG 
CCCAGCAACA CATCCAAAAA GCCTTCAGCT CCTGCATCTA CAAAAACTGC AAAGGATGAA 
GTAGTCCCTC GTGCCACAGC TTGCTCTGTG CAGTCTTCAG CTCCAACATC TGTGTCTTCT 
GTTGGTCACG TAGCTGATGA AGCAGTAGAA GCCTTGTCCA GTAGTCTGGG AGAAAGGGAG 
CCTGATCCAG AAGAAAAGAA ACCTGCTGTG GACAAAGTTA AGGAGAAAGC CAAACGGAAA 
CAACACAAAA AACTGGGTGA AGATGAAGAA ACAATTCCTC CAGAGTACAG ATTAACAGAT 
GCAAAAGATA AAGATGGAAG ACCACTGTTA ATAAAACCTG AAGAGGAGTC CCAGCCTATG 
AGTGAAAACG ATCTGTTAGA AGGTTTGACA AAGGGATTTT CTCCAGCCCA ATCTGCACCA 
TTACCTACAC AAACTGTAAA AAAGAAAACC AAAGGGGGTA AAAAAACTGC AGATTCTTCA 
GATGTTATCT CTGCTTCTAC AATTTCCTCA GTGCACTCAG CAGCTCCCCT AGCTTCTACG 
TCGGGAGGAA AAGAGATGGA TGATGCCTTG GATCTCTTGT CTGATTCCCT GGGACAGAGA 
GAACCTGACC CTGATGAGAA CAAACCAGTT GTGGATAAAG TAAAGGAAAA AGCTAAGTCT 
GAACACAGAG ACAAACTTGG AGAAAGAGAT GATACTATTC CACCTGACTA TCGAAAACTC 
CTGGAGTCAT CTGATGAGGG TAAACCTGTC AAGCCAACAC CAAAGGATGC TTCGAAAAAC 
AAAGAACAAA AGAAGCCTAC AGATGAGTCT GCTGCTATTG ATGCCCTGTC AGGCGACTTT 
GATACTAGTC CAAAGGCTCC TGCCACACCA CAGCACTCAA AGGAGAAAAG TGGAAAGGAA 
ACCGTCACCA CTAAAGCAAC CCCAAAGGAT GAAAGAAAAC CCAAGGATCA TAAAAAGGCA 
AGGGGACAGT CCTCCAGCAG CAAATCTGAG AAGCAGAAAA CAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025000474.1
CDS	16..2523
Translation	MDPDTPPPPCPARSPAGTALLRGSVAAGARPWPLPWLVPPSEGGGCWGAARASVSLAVPCRAVPCGAASSEQRHGFRALVQGDKKASGTSSKSGEKKEATEKPAGANVSQLPKTQTSGVLPAAKKQSPSADAPKPPIMKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKEKSGKETVTTKATPKDERKPKDHKKARGQSSSSKSEKQKTS

Target ORF information:

RefSeq Version	XM_025144706.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025144706.1

ORF Insert Sequence:

ATGGACCCCG ACACCCCCCC CCCCCCGTGC CCGGCCCGCA GTCCCGCCGG CACCGCCCTG 
CTGCGGGGCT CGGTGGCGGC TGGGGCGCGG CCGTGGCCAC TCCCGTGGCT GGTGCCGCCC 
TCAGAGGGAG GTGGCTGCTG GGGCGCGGCC CGTGCGTCTG TGTCCCTTGC CGTGCCGTGC 
CGTGCCGTGC CGTGCGGAGC AGCGAGCAGC GAGCAGCGCC ATGGCTTTCG CGCGCTGGTG 
CAGGGCGACA AGAAGGCAAG TGGAACTTCT TCCAAATCGG GAGAGAAAAA AGAAGCAACA 
GAGAAGCCAG CAGGTGCTAA TGTCAGTCAG CTTCCCAAAA CACAGACAAG TGGAGTACTG 
CCTGCTGCTA AGAAGCAGTC TCCCTCTGCG GATGCACCAA AACCACCCAT TATGAAGCCA 
TCTGAAGCAA AGTCCACACC AACAATCCAA ACAGAACCTG AGAAAACTGC GCTGTCAACA 
AAGCCATCAA ACTCTCAAAA TAATCAACTG GCTGAAGAGA AGCCAAAGGA ATCCAGTGGT 
GCAAAAAGCC CAATTGCACC TTCTGTGGTA ACAGCTTCTA AACCAAATAA TATGGGAAAG 
GAAACTACAA GTCAAGCTGA GCGGCCTTCA CAGGCAACCT CTGCAGACAC AGCACAGCCT 
AAGACTGAAA AGACATCCAC AGCAGGTGGT GCAGGTGCAG CAGGTGCAGG TGCTGTCGCG 
AGTATGGTTG CTGCAGCTGA TAAACCAAAT AGTGAGCCTG CTATGGATGA ATCAGCACTG 
GACAGCTTAA TAGATACGCT AGGTGGATCT GAAGAAGATG TGGCTACTAG ACCAGTTTAC 
ACTGGTCCGG AGATTACGGA AAATATTTCT TCAGAATACT TGGAAGAGCT GGGTAAACGA 
GAAGGTAGCC TCCCTCCAGA GTACCTTGCA TTATTGAAGA GCAAAGTGGA TGGCAAAGAT 
GGAGGCCTAC CAAAAGTAGA TGAACATTCT GAAAAACCGA TGACGGATGA TGAGCTTGCA 
GATGCCCTGT CATCTGACTT TACCTGTAGC ATTGCTGCTG CTGAAGAAAA GAAGACAACA 
GAGAAACAAA CTAAGGAAGG AGAAATCATA CAAGCACAAG TGACAAGTTC AGTGAAGACT 
TCAGTTCCTC CTAAAGAGAA GAAAACAAAA TCAAAAGAGG AAATCAAAGA AGATGCAATG 
GAAGCTCTCA TGGCTACTAT TGGTGGACCT GAACCTGAAC CCGAACCAAA AGATGTTAGC 
CCTATTGTAG AAGTGTCAGA GGCAAAAGCT AAAGAGAAAA AAGAAAAGAA GGCTGGAGAA 
CGTGATGATA CAATACCTCC AGAATACAGG CTAACTCCAG AGCTGGATAA AGATGGAAAA 
CCAGTATTGC CAAAGCCTGA AGAAAAACCT AAGCCTTTGA GTGAATCTGA CCTTGTTGAT 
GAATTTTCAA AAGACTTTGC CAGCCCTGCA CAGCCTGCAG TACAATCCAA ACCATCAAAG 
CCCAGCAACA CATCCAAAAA GCCTTCAGCT CCTGCATCTA CAAAAACTGC AAAGGATGAA 
GTAGTCCCTC GTGCCACAGC TTGCTCTGTG CAGTCTTCAG CTCCAACATC TGTGTCTTCT 
GTTGGTCACG TAGCTGATGA AGCAGTAGAA GCCTTGTCCA GTAGTCTGGG AGAAAGGGAG 
CCTGATCCAG AAGAAAAGAA ACCTGCTGTG GACAAAGTTA AGGAGAAAGC CAAACGGAAA 
CAACACAAAA AACTGGGTGA AGATGAAGAA ACAATTCCTC CAGAGTACAG ATTAACAGAT 
GCAAAAGATA AAGATGGAAG ACCACTGTTA ATAAAACCTG AAGAGGAGTC CCAGCCTATG 
AGTGAAAACG ATCTGTTAGA AGGTTTGACA AAGGGATTTT CTCCAGCCCA ATCTGCACCA 
TTACCTACAC AAACTGTAAA AAAGAAAACC AAAGGGGGTA AAAAAACTGC AGATTCTTCA 
GATGTTATCT CTGCTTCTAC AATTTCCTCA GTGCACTCAG CAGCTCCCCT AGCTTCTACG 
TCGGGAGGAA AAGAGATGGA TGATGCCTTG GATCTCTTGT CTGATTCCCT GGGACAGAGA 
GAACCTGACC CTGATGAGAA CAAACCAGTT GTGGATAAAG TAAAGGAAAA AGCTAAGTCT 
GAACACAGAG ACAAACTTGG AGAAAGAGAT GATACTATTC CACCTGACTA TCGAAAACTC 
CTGGAGTCAT CTGATGAGGG TAAACCTGTC AAGCCAACAC CAAAGGATGC TTCGAAAAAC 
AAAGAACAAA AGAAGCCTAC AGATGAGTCT GCTGCTATTG ATGCCCTGTC AGGCGACTTT 
GATACTAGTC CAAAGGCTCC TGCCACACCA CAGCACTCAA AGGAGAAAAG TGGAAAGGAA 
ACCGTCACCA CTAAAGCAAC CCCAAAGGAT GAAAGAAAAC CCAAGGATCA TAAAAAGGCA 
AGGGGACAGT CCTCCAGCAG CAAATCTGAG AAGCAGAAAA CAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_015280523.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136009.1 Gallus gallus calpastatin (CAST), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa48193
Clone ID Related Accession (Same CDS sequence)	XM_015280523.1
Accession Version	XM_015280523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT 
CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA 
ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT 
AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT 
TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG 
CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA 
GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT 
AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA 
GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA 
AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG 
TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT 
GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT 
ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA 
GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG 
AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT 
GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG 
GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA 
GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA 
GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC 
AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG 
CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT 
TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA 
GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA 
CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA 
GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA 
CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA 
GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA 
AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA 
ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT 
GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC 
AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA 
GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT 
AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA 
GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT 
GCCACACCAC AGCACTCAAA GTGTAGGAGA AAAGTGGAAA GGAAACCGTC ACCACTAAAG 
CAACCCCAAA GGATGAAAGA AAACCCAAGG ATCATAAAAA GGCAAGGGGA CAGTCCTCCA 
GCAGCAAATC TGAGAAGCAG AAAACAAGCT AAACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136009.1
CDS	95..2413
Translation	MSQPGSGPPGPAAQGDKKASGTSSKSGEKKEATEKPAGANVSQLPKTQTSGVLPAAKKQSPSADAPKPPIMKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKCRRKVERKPSPLKQPQRMKENPRIIKRQGDSPPAANLRSRKQAKR

Target ORF information:

RefSeq Version	XM_015280523.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280523.1

ORF Insert Sequence:

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT 
CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA 
ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT 
AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT 
TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG 
CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA 
GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT 
AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA 
GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA 
AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG 
TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT 
GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT 
ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA 
GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG 
AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT 
GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG 
GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA 
GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA 
GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC 
AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG 
CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT 
TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA 
GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA 
CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA 
GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA 
CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA 
GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA 
AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA 
ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT 
GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC 
AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA 
GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT 
AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA 
GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT 
GCCACACCAC AGCACTCAAA GTGTAGGAGA AAAGTGGAAA GGAAACCGTC ACCACTAAAG 
CAACCCCAAA GGATGAAAGA AAACCCAAGG ATCATAAAAA GGCAAGGGGA CAGTCCTCCA 
GCAGCAAATC TGAGAAGCAG AAAACAAGCT AAACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_015280524.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136010.1 Gallus gallus calpastatin (CAST), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa48194
Clone ID Related Accession (Same CDS sequence)	XM_015280524.1
Accession Version	XM_015280524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GCAGTCTCCC 
TCTGCGGATG CACCAAAACC ACCCATTATG AAGCCATCTG AAGCAAAGTC CACACCAACA 
ATCCAAACAG AACCTGAGAA AACTGCGCTG TCAACAAAGC CATCAAACTC TCAAAATAAT 
CAACTGGCTG AAGAGAAGCC AAAGGAATCC AGTGGTGCAA AAAGCCCAAT TGCACCTTCT 
GTGGTAACAG CTTCTAAACC AAATAATATG GGAAAGGAAA CTACAAGTCA AGCTGAGCGG 
CCTTCACAGG CAACCTCTGC AGACACAGCA CAGCCTAAGA CTGAAAAGAC ATCCACAGCA 
GGTGGTGCAG GTGCAGCAGG TGCAGGTGCT GTCGCGAGTA TGGTTGCTGC AGCTGATAAA 
CCAAATAGTG AGCCTGCTAT GGATGAATCA GCACTGGACA GCTTAATAGA TACGCTAGGT 
GGATCTGAAG AAGATGTGGC TACTAGACCA GTTTACACTG GTCCGGAGAT TACGGAAAAT 
ATTTCTTCAG AATACTTGGA AGAGCTGGGT AAACGAGAAG GTAGCCTCCC TCCAGAGTAC 
CTTGCATTAT TGAAGAGCAA AGTGGATGGC AAAGATGGAG GCCTACCAAA AGTAGATGAA 
CATTCTGAAA AACCGATGAC GGATGATGAG CTTGCAGATG CCCTGTCATC TGACTTTACC 
TGTAGCATTG CTGCTGCTGA AGAAAAGAAG ACAACAGAGA AACAAACTAA GGAAGGAGAA 
ATCATACAAG CACAAGTGAC AAGTTCAGTG AAGACTTCAG TTCCTCCTAA AGAGAAGAAA 
ACAAAATCAA AAGAGGAAAT CAAAGAAGAT GCAATGGAAG CTCTCATGGC TACTATTGGT 
GGACCTGAAC CTGAACCCGA ACCAAAAGAT GTTAGCCCTA TTGTAGAAGT GTCAGAGGCA 
AAAGCTAAAG AGAAAAAAGA AAAGAAGGCT GGAGAACGTG ATGATACAAT ACCTCCAGAA 
TACAGGCTAA CTCCAGAGCT GGATAAAGAT GGAAAACCAG TATTGCCAAA GCCTGAAGAA 
AAACCTAAGC CTTTGAGTGA ATCTGACCTT GTTGATGAAT TTTCAAAAGA CTTTGCCAGC 
CCTGCACAGC CTGCAGTACA ATCCAAACCA TCAAAGCCCA GCAACACATC CAAAAAGCCT 
TCAGCTCCTG CATCTACAAA AACTGCAAAG GATGAAGTAG TCCCTCGTGC CACAGCTTGC 
TCTGTGCAGT CTTCAGCTCC AACATCTGTG TCTTCTGTTG GTCACGTAGC TGATGAAGCA 
GTAGAAGCCT TGTCCAGTAG TCTGGGAGAA AGGGAGCCTG ATCCAGAAGA AAAGAAACCT 
GCTGTGGACA AAGTTAAGGA GAAAGCCAAA CGGAAACAAC ACAAAAAACT GGGTGAAGAT 
GAAGAAACAA TTCCTCCAGA GTACAGATTA ACAGATGCAA AAGATAAAGA TGGAAGACCA 
CTGTTAATAA AACCTGAAGA GGAGTCCCAG CCTATGAGTG AAAACGATCT GTTAGAAGGT 
TTGACAAAGG GATTTTCTCC AGCCCAATCT GCACCATTAC CTACACAAAC TGTAAAAAAG 
AAAACCAAAG GGGGTAAAAA AACTGCAGAT TCTTCAGATG TTATCTCTGC TTCTACAATT 
TCCTCAGTGC ACTCAGCAGC TCCCCTAGCT TCTACGTCGG GAGGAAAAGA GATGGATGAT 
GCCTTGGATC TCTTGTCTGA TTCCCTGGGA CAGAGAGAAC CTGACCCTGA TGAGAACAAA 
CCAGTTGTGG ATAAAGTAAA GGAAAAAGCT AAGTCTGAAC ACAGAGACAA ACTTGGAGAA 
AGAGATGATA CTATTCCACC TGACTATCGA AAACTCCTGG AGTCATCTGA TGAGGGTAAA 
CCTGTCAAGC CAACACCAAA GGATGCTTCG AAAAACAAAG AACAAAAGAA GCCTACAGAT 
GAGTCTGCTG CTATTGATGC CCTGTCAGGC GACTTTGATA CTAGTCCAAA GGCTCCTGCC 
ACACCACAGC ACTCAAAGTG TAGGAGAAAA GTGGAAAGGA AACCGTCACC ACTAAAGCAA 
CCCCAAAGGA TGAAAGAAAA CCCAAGGATC ATAAAAAGGC AAGGGGACAG TCCTCCAGCA 
GCAAATCTGA GAAGCAGAAA ACAAGCTAAA CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136010.1
CDS	95..2410
Translation	MSQPGSGPPGPAAQGDKKASGTSSKSGEKKEATEKPAGANVSQLPKTQTSGVLPAAKQSPSADAPKPPIMKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKCRRKVERKPSPLKQPQRMKENPRIIKRQGDSPPAANLRSRKQAKR

Target ORF information:

RefSeq Version	XM_015280524.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280524.1

ORF Insert Sequence:

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GCAGTCTCCC 
TCTGCGGATG CACCAAAACC ACCCATTATG AAGCCATCTG AAGCAAAGTC CACACCAACA 
ATCCAAACAG AACCTGAGAA AACTGCGCTG TCAACAAAGC CATCAAACTC TCAAAATAAT 
CAACTGGCTG AAGAGAAGCC AAAGGAATCC AGTGGTGCAA AAAGCCCAAT TGCACCTTCT 
GTGGTAACAG CTTCTAAACC AAATAATATG GGAAAGGAAA CTACAAGTCA AGCTGAGCGG 
CCTTCACAGG CAACCTCTGC AGACACAGCA CAGCCTAAGA CTGAAAAGAC ATCCACAGCA 
GGTGGTGCAG GTGCAGCAGG TGCAGGTGCT GTCGCGAGTA TGGTTGCTGC AGCTGATAAA 
CCAAATAGTG AGCCTGCTAT GGATGAATCA GCACTGGACA GCTTAATAGA TACGCTAGGT 
GGATCTGAAG AAGATGTGGC TACTAGACCA GTTTACACTG GTCCGGAGAT TACGGAAAAT 
ATTTCTTCAG AATACTTGGA AGAGCTGGGT AAACGAGAAG GTAGCCTCCC TCCAGAGTAC 
CTTGCATTAT TGAAGAGCAA AGTGGATGGC AAAGATGGAG GCCTACCAAA AGTAGATGAA 
CATTCTGAAA AACCGATGAC GGATGATGAG CTTGCAGATG CCCTGTCATC TGACTTTACC 
TGTAGCATTG CTGCTGCTGA AGAAAAGAAG ACAACAGAGA AACAAACTAA GGAAGGAGAA 
ATCATACAAG CACAAGTGAC AAGTTCAGTG AAGACTTCAG TTCCTCCTAA AGAGAAGAAA 
ACAAAATCAA AAGAGGAAAT CAAAGAAGAT GCAATGGAAG CTCTCATGGC TACTATTGGT 
GGACCTGAAC CTGAACCCGA ACCAAAAGAT GTTAGCCCTA TTGTAGAAGT GTCAGAGGCA 
AAAGCTAAAG AGAAAAAAGA AAAGAAGGCT GGAGAACGTG ATGATACAAT ACCTCCAGAA 
TACAGGCTAA CTCCAGAGCT GGATAAAGAT GGAAAACCAG TATTGCCAAA GCCTGAAGAA 
AAACCTAAGC CTTTGAGTGA ATCTGACCTT GTTGATGAAT TTTCAAAAGA CTTTGCCAGC 
CCTGCACAGC CTGCAGTACA ATCCAAACCA TCAAAGCCCA GCAACACATC CAAAAAGCCT 
TCAGCTCCTG CATCTACAAA AACTGCAAAG GATGAAGTAG TCCCTCGTGC CACAGCTTGC 
TCTGTGCAGT CTTCAGCTCC AACATCTGTG TCTTCTGTTG GTCACGTAGC TGATGAAGCA 
GTAGAAGCCT TGTCCAGTAG TCTGGGAGAA AGGGAGCCTG ATCCAGAAGA AAAGAAACCT 
GCTGTGGACA AAGTTAAGGA GAAAGCCAAA CGGAAACAAC ACAAAAAACT GGGTGAAGAT 
GAAGAAACAA TTCCTCCAGA GTACAGATTA ACAGATGCAA AAGATAAAGA TGGAAGACCA 
CTGTTAATAA AACCTGAAGA GGAGTCCCAG CCTATGAGTG AAAACGATCT GTTAGAAGGT 
TTGACAAAGG GATTTTCTCC AGCCCAATCT GCACCATTAC CTACACAAAC TGTAAAAAAG 
AAAACCAAAG GGGGTAAAAA AACTGCAGAT TCTTCAGATG TTATCTCTGC TTCTACAATT 
TCCTCAGTGC ACTCAGCAGC TCCCCTAGCT TCTACGTCGG GAGGAAAAGA GATGGATGAT 
GCCTTGGATC TCTTGTCTGA TTCCCTGGGA CAGAGAGAAC CTGACCCTGA TGAGAACAAA 
CCAGTTGTGG ATAAAGTAAA GGAAAAAGCT AAGTCTGAAC ACAGAGACAA ACTTGGAGAA 
AGAGATGATA CTATTCCACC TGACTATCGA AAACTCCTGG AGTCATCTGA TGAGGGTAAA 
CCTGTCAAGC CAACACCAAA GGATGCTTCG AAAAACAAAG AACAAAAGAA GCCTACAGAT 
GAGTCTGCTG CTATTGATGC CCTGTCAGGC GACTTTGATA CTAGTCCAAA GGCTCCTGCC 
ACACCACAGC ACTCAAAGTG TAGGAGAAAA GTGGAAAGGA AACCGTCACC ACTAAAGCAA 
CCCCAAAGGA TGAAAGAAAA CCCAAGGATC ATAAAAAGGC AAGGGGACAG TCCTCCAGCA 
GCAAATCTGA GAAGCAGAAA ACAAGCTAAA CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_015280525.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136011.1 Gallus gallus calpastatin (CAST), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa48195
Clone ID Related Accession (Same CDS sequence)	XM_015280525.1
Accession Version	XM_015280525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT 
CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA 
ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT 
AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT 
TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG 
CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA 
GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT 
AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA 
GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA 
AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG 
TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT 
GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT 
ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA 
GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG 
AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT 
GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG 
GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA 
GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA 
GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC 
AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG 
CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT 
TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA 
GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA 
CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA 
GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA 
CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA 
GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA 
AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA 
ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT 
GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC 
AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA 
GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT 
AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA 
GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT 
GCCACACCAC AGCACTCAAA GGAGAAAAGT GGAAAGGAAA CCGTCACCAC TAAAGCAACC 
CCAAAGGATG AAAGAAAACC CAAGGATCAT AAAAAGGCAA GGGGACAGTC CTCCAGCAGC 
AAATCTGAGA AGCAGAAAAC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136011.1
CDS	95..2401
Translation	MSQPGSGPPGPAAQGDKKASGTSSKSGEKKEATEKPAGANVSQLPKTQTSGVLPAAKKQSPSADAPKPPIMKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKEKSGKETVTTKATPKDERKPKDHKKARGQSSSSKSEKQKTS

Target ORF information:

RefSeq Version	XM_015280525.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280525.1

ORF Insert Sequence:

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT 
CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA 
ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT 
AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT 
TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG 
CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA 
GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT 
AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA 
GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA 
AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG 
TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT 
GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT 
ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA 
GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG 
AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT 
GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG 
GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA 
GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA 
GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC 
AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG 
CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT 
TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA 
GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA 
CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC AAACGGAAAC AACACAAAAA ACTGGGTGAA 
GATGAAGAAA CAATTCCTCC AGAGTACAGA TTAACAGATG CAAAAGATAA AGATGGAAGA 
CCACTGTTAA TAAAACCTGA AGAGGAGTCC CAGCCTATGA GTGAAAACGA TCTGTTAGAA 
GGTTTGACAA AGGGATTTTC TCCAGCCCAA TCTGCACCAT TACCTACACA AACTGTAAAA 
AAGAAAACCA AAGGGGGTAA AAAAACTGCA GATTCTTCAG ATGTTATCTC TGCTTCTACA 
ATTTCCTCAG TGCACTCAGC AGCTCCCCTA GCTTCTACGT CGGGAGGAAA AGAGATGGAT 
GATGCCTTGG ATCTCTTGTC TGATTCCCTG GGACAGAGAG AACCTGACCC TGATGAGAAC 
AAACCAGTTG TGGATAAAGT AAAGGAAAAA GCTAAGTCTG AACACAGAGA CAAACTTGGA 
GAAAGAGATG ATACTATTCC ACCTGACTAT CGAAAACTCC TGGAGTCATC TGATGAGGGT 
AAACCTGTCA AGCCAACACC AAAGGATGCT TCGAAAAACA AAGAACAAAA GAAGCCTACA 
GATGAGTCTG CTGCTATTGA TGCCCTGTCA GGCGACTTTG ATACTAGTCC AAAGGCTCCT 
GCCACACCAC AGCACTCAAA GGAGAAAAGT GGAAAGGAAA CCGTCACCAC TAAAGCAACC 
CCAAAGGATG AAAGAAAACC CAAGGATCAT AAAAAGGCAA GGGGACAGTC CTCCAGCAGC 
AAATCTGAGA AGCAGAAAAC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_015280526.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136012.1 Gallus gallus calpastatin (CAST), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa48196
Clone ID Related Accession (Same CDS sequence)	XM_015280526.1
Accession Version	XM_015280526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCAGTC TCCCTCTGCG 
GATGCACCAA AACCACCCAT TATGAAGCCA TCTGAAGCAA AGTCCACACC AACAATCCAA 
ACAGAACCTG AGAAAACTGC GCTGTCAACA AAGCCATCAA ACTCTCAAAA TAATCAACTG 
GCTGAAGAGA AGCCAAAGGA ATCCAGTGGT GCAAAAAGCC CAATTGCACC TTCTGTGGTA 
ACAGCTTCTA AACCAAATAA TATGGGAAAG GAAACTACAA GTCAAGCTGA GCGGCCTTCA 
CAGGCAACCT CTGCAGACAC AGCACAGCCT AAGACTGAAA AGACATCCAC AGCAGGTGGT 
GCAGGTGCAG CAGGTGCAGG TGCTGTCGCG AGTATGGTTG CTGCAGCTGA TAAACCAAAT 
AGTGAGCCTG CTATGGATGA ATCAGCACTG GACAGCTTAA TAGATACGCT AGGTGGATCT 
GAAGAAGATG TGGCTACTAG ACCAGTTTAC ACTGGTCCGG AGATTACGGA AAATATTTCT 
TCAGAATACT TGGAAGAGCT GGGTAAACGA GAAGGTAGCC TCCCTCCAGA GTACCTTGCA 
TTATTGAAGA GCAAAGTGGA TGGCAAAGAT GGAGGCCTAC CAAAAGTAGA TGAACATTCT 
GAAAAACCGA TGACGGATGA TGAGCTTGCA GATGCCCTGT CATCTGACTT TACCTGTAGC 
ATTGCTGCTG CTGAAGAAAA GAAGACAACA GAGAAACAAA CTAAGGAAGG AGAAATCATA 
CAAGCACAAG TGACAAGTTC AGTGAAGACT TCAGTTCCTC CTAAAGAGAA GAAAACAAAA 
TCAAAAGAGG AAATCAAAGA AGATGCAATG GAAGCTCTCA TGGCTACTAT TGGTGGACCT 
GAACCTGAAC CCGAACCAAA AGATGTTAGC CCTATTGTAG AAGTGTCAGA GGCAAAAGCT 
AAAGAGAAAA AAGAAAAGAA GGCTGGAGAA CGTGATGATA CAATACCTCC AGAATACAGG 
CTAACTCCAG AGCTGGATAA AGATGGAAAA CCAGTATTGC CAAAGCCTGA AGAAAAACCT 
AAGCCTTTGA GTGAATCTGA CCTTGTTGAT GAATTTTCAA AAGACTTTGC CAGCCCTGCA 
CAGCCTGCAG TACAATCCAA ACCATCAAAG CCCAGCAACA CATCCAAAAA GCCTTCAGCT 
CCTGCATCTA CAAAAACTGC AAAGGATGAA GTAGTCCCTC GTGCCACAGC TTGCTCTGTG 
CAGTCTTCAG CTCCAACATC TGTGTCTTCT GTTGGTCACG TAGCTGATGA AGCAGTAGAA 
GCCTTGTCCA GTAGTCTGGG AGAAAGGGAG CCTGATCCAG AAGAAAAGAA ACCTGCTGTG 
GACAAAGTTA AGGAGAAAGC CAAACGGAAA CAACACAAAA AACTGGGTGA AGATGAAGAA 
ACAATTCCTC CAGAGTACAG ATTAACAGAT GCAAAAGATA AAGATGGAAG ACCACTGTTA 
ATAAAACCTG AAGAGGAGTC CCAGCCTATG AGTGAAAACG ATCTGTTAGA AGGTTTGACA 
AAGGGATTTT CTCCAGCCCA ATCTGCACCA TTACCTACAC AAACTGTAAA AAAGAAAACC 
AAAGGGGGTA AAAAAACTGC AGATTCTTCA GATGTTATCT CTGCTTCTAC AATTTCCTCA 
GTGCACTCAG CAGCTCCCCT AGCTTCTACG TCGGGAGGAA AAGAGATGGA TGATGCCTTG 
GATCTCTTGT CTGATTCCCT GGGACAGAGA GAACCTGACC CTGATGAGAA CAAACCAGTT 
GTGGATAAAG TAAAGGAAAA AGCTAAGTCT GAACACAGAG ACAAACTTGG AGAAAGAGAT 
GATACTATTC CACCTGACTA TCGAAAACTC CTGGAGTCAT CTGATGAGGG TAAACCTGTC 
AAGCCAACAC CAAAGGATGC TTCGAAAAAC AAAGAACAAA AGAAGCCTAC AGATGAGTCT 
GCTGCTATTG ATGCCCTGTC AGGCGACTTT GATACTAGTC CAAAGGCTCC TGCCACACCA 
CAGCACTCAA AGTGTAGGAG AAAAGTGGAA AGGAAACCGT CACCACTAAA GCAACCCCAA 
AGGATGAAAG AAAACCCAAG GATCATAAAA AGGCAAGGGG ACAGTCCTCC AGCAGCAAAT 
CTGAGAAGCA GAAAACAAGC TAAACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136012.1
CDS	95..2344
Translation	MSQPGSGPPGPAAQGDKKASGTSSKSGEKKEATEKQSPSADAPKPPIMKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKCRRKVERKPSPLKQPQRMKENPRIIKRQGDSPPAANLRSRKQAKR

Target ORF information:

RefSeq Version	XM_015280526.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280526.1

ORF Insert Sequence:

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCAGTC TCCCTCTGCG 
GATGCACCAA AACCACCCAT TATGAAGCCA TCTGAAGCAA AGTCCACACC AACAATCCAA 
ACAGAACCTG AGAAAACTGC GCTGTCAACA AAGCCATCAA ACTCTCAAAA TAATCAACTG 
GCTGAAGAGA AGCCAAAGGA ATCCAGTGGT GCAAAAAGCC CAATTGCACC TTCTGTGGTA 
ACAGCTTCTA AACCAAATAA TATGGGAAAG GAAACTACAA GTCAAGCTGA GCGGCCTTCA 
CAGGCAACCT CTGCAGACAC AGCACAGCCT AAGACTGAAA AGACATCCAC AGCAGGTGGT 
GCAGGTGCAG CAGGTGCAGG TGCTGTCGCG AGTATGGTTG CTGCAGCTGA TAAACCAAAT 
AGTGAGCCTG CTATGGATGA ATCAGCACTG GACAGCTTAA TAGATACGCT AGGTGGATCT 
GAAGAAGATG TGGCTACTAG ACCAGTTTAC ACTGGTCCGG AGATTACGGA AAATATTTCT 
TCAGAATACT TGGAAGAGCT GGGTAAACGA GAAGGTAGCC TCCCTCCAGA GTACCTTGCA 
TTATTGAAGA GCAAAGTGGA TGGCAAAGAT GGAGGCCTAC CAAAAGTAGA TGAACATTCT 
GAAAAACCGA TGACGGATGA TGAGCTTGCA GATGCCCTGT CATCTGACTT TACCTGTAGC 
ATTGCTGCTG CTGAAGAAAA GAAGACAACA GAGAAACAAA CTAAGGAAGG AGAAATCATA 
CAAGCACAAG TGACAAGTTC AGTGAAGACT TCAGTTCCTC CTAAAGAGAA GAAAACAAAA 
TCAAAAGAGG AAATCAAAGA AGATGCAATG GAAGCTCTCA TGGCTACTAT TGGTGGACCT 
GAACCTGAAC CCGAACCAAA AGATGTTAGC CCTATTGTAG AAGTGTCAGA GGCAAAAGCT 
AAAGAGAAAA AAGAAAAGAA GGCTGGAGAA CGTGATGATA CAATACCTCC AGAATACAGG 
CTAACTCCAG AGCTGGATAA AGATGGAAAA CCAGTATTGC CAAAGCCTGA AGAAAAACCT 
AAGCCTTTGA GTGAATCTGA CCTTGTTGAT GAATTTTCAA AAGACTTTGC CAGCCCTGCA 
CAGCCTGCAG TACAATCCAA ACCATCAAAG CCCAGCAACA CATCCAAAAA GCCTTCAGCT 
CCTGCATCTA CAAAAACTGC AAAGGATGAA GTAGTCCCTC GTGCCACAGC TTGCTCTGTG 
CAGTCTTCAG CTCCAACATC TGTGTCTTCT GTTGGTCACG TAGCTGATGA AGCAGTAGAA 
GCCTTGTCCA GTAGTCTGGG AGAAAGGGAG CCTGATCCAG AAGAAAAGAA ACCTGCTGTG 
GACAAAGTTA AGGAGAAAGC CAAACGGAAA CAACACAAAA AACTGGGTGA AGATGAAGAA 
ACAATTCCTC CAGAGTACAG ATTAACAGAT GCAAAAGATA AAGATGGAAG ACCACTGTTA 
ATAAAACCTG AAGAGGAGTC CCAGCCTATG AGTGAAAACG ATCTGTTAGA AGGTTTGACA 
AAGGGATTTT CTCCAGCCCA ATCTGCACCA TTACCTACAC AAACTGTAAA AAAGAAAACC 
AAAGGGGGTA AAAAAACTGC AGATTCTTCA GATGTTATCT CTGCTTCTAC AATTTCCTCA 
GTGCACTCAG CAGCTCCCCT AGCTTCTACG TCGGGAGGAA AAGAGATGGA TGATGCCTTG 
GATCTCTTGT CTGATTCCCT GGGACAGAGA GAACCTGACC CTGATGAGAA CAAACCAGTT 
GTGGATAAAG TAAAGGAAAA AGCTAAGTCT GAACACAGAG ACAAACTTGG AGAAAGAGAT 
GATACTATTC CACCTGACTA TCGAAAACTC CTGGAGTCAT CTGATGAGGG TAAACCTGTC 
AAGCCAACAC CAAAGGATGC TTCGAAAAAC AAAGAACAAA AGAAGCCTAC AGATGAGTCT 
GCTGCTATTG ATGCCCTGTC AGGCGACTTT GATACTAGTC CAAAGGCTCC TGCCACACCA 
CAGCACTCAA AGTGTAGGAG AAAAGTGGAA AGGAAACCGT CACCACTAAA GCAACCCCAA 
AGGATGAAAG AAAACCCAAG GATCATAAAA AGGCAAGGGG ACAGTCCTCC AGCAGCAAAT 
CTGAGAAGCA GAAAACAAGC TAAACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_015280527.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136013.1 Gallus gallus calpastatin (CAST), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa48197
Clone ID Related Accession (Same CDS sequence)	XM_015280527.1
Accession Version	XM_015280527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGCAGTCTCC CTCTGCGGAT 
GCACCAAAAC CACCCATTAT GAAGCCATCT GAAGCAAAGT CCACACCAAC AATCCAAACA 
GAACCTGAGA AAACTGCGCT GTCAACAAAG CCATCAAACT CTCAAAATAA TCAACTGGCT 
GAAGAGAAGC CAAAGGAATC CAGTGGTGCA AAAAGCCCAA TTGCACCTTC TGTGGTAACA 
GCTTCTAAAC CAAATAATAT GGGAAAGGAA ACTACAAGTC AAGCTGAGCG GCCTTCACAG 
GCAACCTCTG CAGACACAGC ACAGCCTAAG ACTGAAAAGA CATCCACAGC AGGTGGTGCA 
GGTGCAGCAG GTGCAGGTGC TGTCGCGAGT ATGGTTGCTG CAGCTGATAA ACCAAATAGT 
GAGCCTGCTA TGGATGAATC AGCACTGGAC AGCTTAATAG ATACGCTAGG TGGATCTGAA 
GAAGATGTGG CTACTAGACC AGTTTACACT GGTCCGGAGA TTACGGAAAA TATTTCTTCA 
GAATACTTGG AAGAGCTGGG TAAACGAGAA GGTAGCCTCC CTCCAGAGTA CCTTGCATTA 
TTGAAGAGCA AAGTGGATGG CAAAGATGGA GGCCTACCAA AAGTAGATGA ACATTCTGAA 
AAACCGATGA CGGATGATGA GCTTGCAGAT GCCCTGTCAT CTGACTTTAC CTGTAGCATT 
GCTGCTGCTG AAGAAAAGAA GACAACAGAG AAACAAACTA AGGAAGGAGA AATCATACAA 
GCACAAGTGA CAAGTTCAGT GAAGACTTCA GTTCCTCCTA AAGAGAAGAA AACAAAATCA 
AAAGAGGAAA TCAAAGAAGA TGCAATGGAA GCTCTCATGG CTACTATTGG TGGACCTGAA 
CCTGAACCCG AACCAAAAGA TGTTAGCCCT ATTGTAGAAG TGTCAGAGGC AAAAGCTAAA 
GAGAAAAAAG AAAAGAAGGC TGGAGAACGT GATGATACAA TACCTCCAGA ATACAGGCTA 
ACTCCAGAGC TGGATAAAGA TGGAAAACCA GTATTGCCAA AGCCTGAAGA AAAACCTAAG 
CCTTTGAGTG AATCTGACCT TGTTGATGAA TTTTCAAAAG ACTTTGCCAG CCCTGCACAG 
CCTGCAGTAC AATCCAAACC ATCAAAGCCC AGCAACACAT CCAAAAAGCC TTCAGCTCCT 
GCATCTACAA AAACTGCAAA GGATGAAGTA GTCCCTCGTG CCACAGCTTG CTCTGTGCAG 
TCTTCAGCTC CAACATCTGT GTCTTCTGTT GGTCACGTAG CTGATGAAGC AGTAGAAGCC 
TTGTCCAGTA GTCTGGGAGA AAGGGAGCCT GATCCAGAAG AAAAGAAACC TGCTGTGGAC 
AAAGTTAAGG AGAAAGCCAA ACGGAAACAA CACAAAAAAC TGGGTGAAGA TGAAGAAACA 
ATTCCTCCAG AGTACAGATT AACAGATGCA AAAGATAAAG ATGGAAGACC ACTGTTAATA 
AAACCTGAAG AGGAGTCCCA GCCTATGAGT GAAAACGATC TGTTAGAAGG TTTGACAAAG 
GGATTTTCTC CAGCCCAATC TGCACCATTA CCTACACAAA CTGTAAAAAA GAAAACCAAA 
GGGGGTAAAA AAACTGCAGA TTCTTCAGAT GTTATCTCTG CTTCTACAAT TTCCTCAGTG 
CACTCAGCAG CTCCCCTAGC TTCTACGTCG GGAGGAAAAG AGATGGATGA TGCCTTGGAT 
CTCTTGTCTG ATTCCCTGGG ACAGAGAGAA CCTGACCCTG ATGAGAACAA ACCAGTTGTG 
GATAAAGTAA AGGAAAAAGC TAAGTCTGAA CACAGAGACA AACTTGGAGA AAGAGATGAT 
ACTATTCCAC CTGACTATCG AAAACTCCTG GAGTCATCTG ATGAGGGTAA ACCTGTCAAG 
CCAACACCAA AGGATGCTTC GAAAAACAAA GAACAAAAGA AGCCTACAGA TGAGTCTGCT 
GCTATTGATG CCCTGTCAGG CGACTTTGAT ACTAGTCCAA AGGCTCCTGC CACACCACAG 
CACTCAAAGT GTAGGAGAAA AGTGGAAAGG AAACCGTCAC CACTAAAGCA ACCCCAAAGG 
ATGAAAGAAA ACCCAAGGAT CATAAAAAGG CAAGGGGACA GTCCTCCAGC AGCAAATCTG 
AGAAGCAGAA AACAAGCTAA ACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136013.1
CDS	95..2341
Translation	MSQPGSGPPGPAAQGDKKASGTSSKSGEKKEATEQSPSADAPKPPIMKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKCRRKVERKPSPLKQPQRMKENPRIIKRQGDSPPAANLRSRKQAKR

Target ORF information:

RefSeq Version	XM_015280527.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280527.1

ORF Insert Sequence:

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGCAGTCTCC CTCTGCGGAT 
GCACCAAAAC CACCCATTAT GAAGCCATCT GAAGCAAAGT CCACACCAAC AATCCAAACA 
GAACCTGAGA AAACTGCGCT GTCAACAAAG CCATCAAACT CTCAAAATAA TCAACTGGCT 
GAAGAGAAGC CAAAGGAATC CAGTGGTGCA AAAAGCCCAA TTGCACCTTC TGTGGTAACA 
GCTTCTAAAC CAAATAATAT GGGAAAGGAA ACTACAAGTC AAGCTGAGCG GCCTTCACAG 
GCAACCTCTG CAGACACAGC ACAGCCTAAG ACTGAAAAGA CATCCACAGC AGGTGGTGCA 
GGTGCAGCAG GTGCAGGTGC TGTCGCGAGT ATGGTTGCTG CAGCTGATAA ACCAAATAGT 
GAGCCTGCTA TGGATGAATC AGCACTGGAC AGCTTAATAG ATACGCTAGG TGGATCTGAA 
GAAGATGTGG CTACTAGACC AGTTTACACT GGTCCGGAGA TTACGGAAAA TATTTCTTCA 
GAATACTTGG AAGAGCTGGG TAAACGAGAA GGTAGCCTCC CTCCAGAGTA CCTTGCATTA 
TTGAAGAGCA AAGTGGATGG CAAAGATGGA GGCCTACCAA AAGTAGATGA ACATTCTGAA 
AAACCGATGA CGGATGATGA GCTTGCAGAT GCCCTGTCAT CTGACTTTAC CTGTAGCATT 
GCTGCTGCTG AAGAAAAGAA GACAACAGAG AAACAAACTA AGGAAGGAGA AATCATACAA 
GCACAAGTGA CAAGTTCAGT GAAGACTTCA GTTCCTCCTA AAGAGAAGAA AACAAAATCA 
AAAGAGGAAA TCAAAGAAGA TGCAATGGAA GCTCTCATGG CTACTATTGG TGGACCTGAA 
CCTGAACCCG AACCAAAAGA TGTTAGCCCT ATTGTAGAAG TGTCAGAGGC AAAAGCTAAA 
GAGAAAAAAG AAAAGAAGGC TGGAGAACGT GATGATACAA TACCTCCAGA ATACAGGCTA 
ACTCCAGAGC TGGATAAAGA TGGAAAACCA GTATTGCCAA AGCCTGAAGA AAAACCTAAG 
CCTTTGAGTG AATCTGACCT TGTTGATGAA TTTTCAAAAG ACTTTGCCAG CCCTGCACAG 
CCTGCAGTAC AATCCAAACC ATCAAAGCCC AGCAACACAT CCAAAAAGCC TTCAGCTCCT 
GCATCTACAA AAACTGCAAA GGATGAAGTA GTCCCTCGTG CCACAGCTTG CTCTGTGCAG 
TCTTCAGCTC CAACATCTGT GTCTTCTGTT GGTCACGTAG CTGATGAAGC AGTAGAAGCC 
TTGTCCAGTA GTCTGGGAGA AAGGGAGCCT GATCCAGAAG AAAAGAAACC TGCTGTGGAC 
AAAGTTAAGG AGAAAGCCAA ACGGAAACAA CACAAAAAAC TGGGTGAAGA TGAAGAAACA 
ATTCCTCCAG AGTACAGATT AACAGATGCA AAAGATAAAG ATGGAAGACC ACTGTTAATA 
AAACCTGAAG AGGAGTCCCA GCCTATGAGT GAAAACGATC TGTTAGAAGG TTTGACAAAG 
GGATTTTCTC CAGCCCAATC TGCACCATTA CCTACACAAA CTGTAAAAAA GAAAACCAAA 
GGGGGTAAAA AAACTGCAGA TTCTTCAGAT GTTATCTCTG CTTCTACAAT TTCCTCAGTG 
CACTCAGCAG CTCCCCTAGC TTCTACGTCG GGAGGAAAAG AGATGGATGA TGCCTTGGAT 
CTCTTGTCTG ATTCCCTGGG ACAGAGAGAA CCTGACCCTG ATGAGAACAA ACCAGTTGTG 
GATAAAGTAA AGGAAAAAGC TAAGTCTGAA CACAGAGACA AACTTGGAGA AAGAGATGAT 
ACTATTCCAC CTGACTATCG AAAACTCCTG GAGTCATCTG ATGAGGGTAA ACCTGTCAAG 
CCAACACCAA AGGATGCTTC GAAAAACAAA GAACAAAAGA AGCCTACAGA TGAGTCTGCT 
GCTATTGATG CCCTGTCAGG CGACTTTGAT ACTAGTCCAA AGGCTCCTGC CACACCACAG 
CACTCAAAGT GTAGGAGAAA AGTGGAAAGG AAACCGTCAC CACTAAAGCA ACCCCAAAGG 
ATGAAAGAAA ACCCAAGGAT CATAAAAAGG CAAGGGGACA GTCCTCCAGC AGCAAATCTG 
AGAAGCAGAA AACAAGCTAA ACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_015280528.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136014.1 Gallus gallus calpastatin (CAST), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa48198
Clone ID Related Accession (Same CDS sequence)	XM_015280528.1
Accession Version	XM_015280528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT 
CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA 
ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT 
AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT 
TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG 
CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA 
GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT 
AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA 
GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA 
AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG 
TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT 
GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT 
ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA 
GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG 
AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT 
GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG 
GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA 
GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA 
GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC 
AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG 
CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT 
TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA 
GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA 
CCTGCTGTGG ACAAAGTTAA GGATAAAGAT GGAAGACCAC TGTTAATAAA ACCTGAAGAG 
GAGTCCCAGC CTATGAGTGA AAACGATCTG TTAGAAGGTT TGACAAAGGG ATTTTCTCCA 
GCCCAATCTG CACCATTACC TACACAAACT GTAAAAAAGA AAACCAAAGG GGGTAAAAAA 
ACTGCAGATT CTTCAGATGT TATCTCTGCT TCTACAATTT CCTCAGTGCA CTCAGCAGCT 
CCCCTAGCTT CTACGTCGGG AGGAAAAGAG ATGGATGATG CCTTGGATCT CTTGTCTGAT 
TCCCTGGGAC AGAGAGAACC TGACCCTGAT GAGAACAAAC CAGTTGTGGA TAAAGTAAAG 
GAAAAAGCTA AGTCTGAACA CAGAGACAAA CTTGGAGAAA GAGATGATAC TATTCCACCT 
GACTATCGAA AACTCCTGGA GTCATCTGAT GAGGGTAAAC CTGTCAAGCC AACACCAAAG 
GATGCTTCGA AAAACAAAGA ACAAAAGAAG CCTACAGATG AGTCTGCTGC TATTGATGCC 
CTGTCAGGCG ACTTTGATAC TAGTCCAAAG GCTCCTGCCA CACCACAGCA CTCAAAGTGT 
AGGAGAAAAG TGGAAAGGAA ACCGTCACCA CTAAAGCAAC CCCAAAGGAT GAAAGAAAAC 
CCAAGGATCA TAAAAAGGCA AGGGGACAGT CCTCCAGCAG CAAATCTGAG AAGCAGAAAA 
CAAGCTAAAC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136014.1
CDS	95..2329
Translation	MSQPGSGPPGPAAQGDKKASGTSSKSGEKKEATEKPAGANVSQLPKTQTSGVLPAAKKQSPSADAPKPPIMKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKCRRKVERKPSPLKQPQRMKENPRIIKRQGDSPPAANLRSRKQAKR

Target ORF information:

RefSeq Version	XM_015280528.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280528.1

ORF Insert Sequence:

ATGTCCCAGC CTGGCTCGGG GCCCCCCGGC CCCGCCGCGC AGGGCGACAA GAAGGCAAGT 
GGAACTTCTT CCAAATCGGG AGAGAAAAAA GAAGCAACAG AGAAGCCAGC AGGTGCTAAT 
GTCAGTCAGC TTCCCAAAAC ACAGACAAGT GGAGTACTGC CTGCTGCTAA GAAGCAGTCT 
CCCTCTGCGG ATGCACCAAA ACCACCCATT ATGAAGCCAT CTGAAGCAAA GTCCACACCA 
ACAATCCAAA CAGAACCTGA GAAAACTGCG CTGTCAACAA AGCCATCAAA CTCTCAAAAT 
AATCAACTGG CTGAAGAGAA GCCAAAGGAA TCCAGTGGTG CAAAAAGCCC AATTGCACCT 
TCTGTGGTAA CAGCTTCTAA ACCAAATAAT ATGGGAAAGG AAACTACAAG TCAAGCTGAG 
CGGCCTTCAC AGGCAACCTC TGCAGACACA GCACAGCCTA AGACTGAAAA GACATCCACA 
GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT GCTGTCGCGA GTATGGTTGC TGCAGCTGAT 
AAACCAAATA GTGAGCCTGC TATGGATGAA TCAGCACTGG ACAGCTTAAT AGATACGCTA 
GGTGGATCTG AAGAAGATGT GGCTACTAGA CCAGTTTACA CTGGTCCGGA GATTACGGAA 
AATATTTCTT CAGAATACTT GGAAGAGCTG GGTAAACGAG AAGGTAGCCT CCCTCCAGAG 
TACCTTGCAT TATTGAAGAG CAAAGTGGAT GGCAAAGATG GAGGCCTACC AAAAGTAGAT 
GAACATTCTG AAAAACCGAT GACGGATGAT GAGCTTGCAG ATGCCCTGTC ATCTGACTTT 
ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG AAGACAACAG AGAAACAAAC TAAGGAAGGA 
GAAATCATAC AAGCACAAGT GACAAGTTCA GTGAAGACTT CAGTTCCTCC TAAAGAGAAG 
AAAACAAAAT CAAAAGAGGA AATCAAAGAA GATGCAATGG AAGCTCTCAT GGCTACTATT 
GGTGGACCTG AACCTGAACC CGAACCAAAA GATGTTAGCC CTATTGTAGA AGTGTCAGAG 
GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG GCTGGAGAAC GTGATGATAC AATACCTCCA 
GAATACAGGC TAACTCCAGA GCTGGATAAA GATGGAAAAC CAGTATTGCC AAAGCCTGAA 
GAAAAACCTA AGCCTTTGAG TGAATCTGAC CTTGTTGATG AATTTTCAAA AGACTTTGCC 
AGCCCTGCAC AGCCTGCAGT ACAATCCAAA CCATCAAAGC CCAGCAACAC ATCCAAAAAG 
CCTTCAGCTC CTGCATCTAC AAAAACTGCA AAGGATGAAG TAGTCCCTCG TGCCACAGCT 
TGCTCTGTGC AGTCTTCAGC TCCAACATCT GTGTCTTCTG TTGGTCACGT AGCTGATGAA 
GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA GAAAGGGAGC CTGATCCAGA AGAAAAGAAA 
CCTGCTGTGG ACAAAGTTAA GGATAAAGAT GGAAGACCAC TGTTAATAAA ACCTGAAGAG 
GAGTCCCAGC CTATGAGTGA AAACGATCTG TTAGAAGGTT TGACAAAGGG ATTTTCTCCA 
GCCCAATCTG CACCATTACC TACACAAACT GTAAAAAAGA AAACCAAAGG GGGTAAAAAA 
ACTGCAGATT CTTCAGATGT TATCTCTGCT TCTACAATTT CCTCAGTGCA CTCAGCAGCT 
CCCCTAGCTT CTACGTCGGG AGGAAAAGAG ATGGATGATG CCTTGGATCT CTTGTCTGAT 
TCCCTGGGAC AGAGAGAACC TGACCCTGAT GAGAACAAAC CAGTTGTGGA TAAAGTAAAG 
GAAAAAGCTA AGTCTGAACA CAGAGACAAA CTTGGAGAAA GAGATGATAC TATTCCACCT 
GACTATCGAA AACTCCTGGA GTCATCTGAT GAGGGTAAAC CTGTCAAGCC AACACCAAAG 
GATGCTTCGA AAAACAAAGA ACAAAAGAAG CCTACAGATG AGTCTGCTGC TATTGATGCC 
CTGTCAGGCG ACTTTGATAC TAGTCCAAAG GCTCCTGCCA CACCACAGCA CTCAAAGTGT 
AGGAGAAAAG TGGAAAGGAA ACCGTCACCA CTAAAGCAAC CCCAAAGGAT GAAAGAAAAC 
CCAAGGATCA TAAAAAGGCA AGGGGACAGT CCTCCAGCAG CAAATCTGAG AAGCAGAAAA 
CAAGCTAAAC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_015280529.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136015.1 Gallus gallus calpastatin (CAST), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa48199
Clone ID Related Accession (Same CDS sequence)	XM_015280529.1 , XM_015280529.2
Accession Version	XM_015280529.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764320.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCAT CTGAAGCAAA GTCCACACCA ACAATCCAAA CAGAACCTGA GAAAACTGCG 
CTGTCAACAA AGCCATCAAA CTCTCAAAAT AATCAACTGG CTGAAGAGAA GCCAAAGGAA 
TCCAGTGGTG CAAAAAGCCC AATTGCACCT TCTGTGGTAA CAGCTTCTAA ACCAAATAAT 
ATGGGAAAGG AAACTACAAG TCAAGCTGAG CGGCCTTCAC AGGCAACCTC TGCAGACACA 
GCACAGCCTA AGACTGAAAA GACATCCACA GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT 
GCTGTCGCGA GTATGGTTGC TGCAGCTGAT AAACCAAATA GTGAGCCTGC TATGGATGAA 
TCAGCACTGG ACAGCTTAAT AGATACGCTA GGTGGATCTG AAGAAGATGT GGCTACTAGA 
CCAGTTTACA CTGGTCCGGA GATTACGGAA AATATTTCTT CAGAATACTT GGAAGAGCTG 
GGTAAACGAG AAGGTAGCCT CCCTCCAGAG TACCTTGCAT TATTGAAGAG CAAAGTGGAT 
GGCAAAGATG GAGGCCTACC AAAAGTAGAT GAACATTCTG AAAAACCGAT GACGGATGAT 
GAGCTTGCAG ATGCCCTGTC ATCTGACTTT ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG 
AAGACAACAG AGAAACAAAC TAAGGAAGGA GAAATCATAC AAGCACAAGT GACAAGTTCA 
GTGAAGACTT CAGTTCCTCC TAAAGAGAAG AAAACAAAAT CAAAAGAGGA AATCAAAGAA 
GATGCAATGG AAGCTCTCAT GGCTACTATT GGTGGACCTG AACCTGAACC CGAACCAAAA 
GATGTTAGCC CTATTGTAGA AGTGTCAGAG GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG 
GCTGGAGAAC GTGATGATAC AATACCTCCA GAATACAGGC TAACTCCAGA GCTGGATAAA 
GATGGAAAAC CAGTATTGCC AAAGCCTGAA GAAAAACCTA AGCCTTTGAG TGAATCTGAC 
CTTGTTGATG AATTTTCAAA AGACTTTGCC AGCCCTGCAC AGCCTGCAGT ACAATCCAAA 
CCATCAAAGC CCAGCAACAC ATCCAAAAAG CCTTCAGCTC CTGCATCTAC AAAAACTGCA 
AAGGATGAAG TAGTCCCTCG TGCCACAGCT TGCTCTGTGC AGTCTTCAGC TCCAACATCT 
GTGTCTTCTG TTGGTCACGT AGCTGATGAA GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA 
GAAAGGGAGC CTGATCCAGA AGAAAAGAAA CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC 
AAACGGAAAC AACACAAAAA ACTGGGTGAA GATGAAGAAA CAATTCCTCC AGAGTACAGA 
TTAACAGATG CAAAAGATAA AGATGGAAGA CCACTGTTAA TAAAACCTGA AGAGGAGTCC 
CAGCCTATGA GTGAAAACGA TCTGTTAGAA GGTTTGACAA AGGGATTTTC TCCAGCCCAA 
TCTGCACCAT TACCTACACA AACTGTAAAA AAGAAAACCA AAGGGGGTAA AAAAACTGCA 
GATTCTTCAG ATGTTATCTC TGCTTCTACA ATTTCCTCAG TGCACTCAGC AGCTCCCCTA 
GCTTCTACGT CGGGAGGAAA AGAGATGGAT GATGCCTTGG ATCTCTTGTC TGATTCCCTG 
GGACAGAGAG AACCTGACCC TGATGAGAAC AAACCAGTTG TGGATAAAGT AAAGGAAAAA 
GCTAAGTCTG AACACAGAGA CAAACTTGGA GAAAGAGATG ATACTATTCC ACCTGACTAT 
CGAAAACTCC TGGAGTCATC TGATGAGGGT AAACCTGTCA AGCCAACACC AAAGGATGCT 
TCGAAAAACA AAGAACAAAA GAAGCCTACA GATGAGTCTG CTGCTATTGA TGCCCTGTCA 
GGCGACTTTG ATACTAGTCC AAAGGCTCCT GCCACACCAC AGCACTCAAA GTGTAGGAGA 
AAAGTGGAAA GGAAACCGTC ACCACTAAAG CAACCCCAAA GGATGAAAGA AAACCCAAGG 
ATCATAAAAA GGCAAGGGGA CAGTCCTCCA GCAGCAAATC TGAGAAGCAG AAAACAAGCT 
AAACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136015.1
CDS	147..2255
Translation	MKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKCRRKVERKPSPLKQPQRMKENPRIIKRQGDSPPAANLRSRKQAKR

Target ORF information:

RefSeq Version	XM_015280529.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280529.1

ORF Insert Sequence:

ATGAAGCCAT CTGAAGCAAA GTCCACACCA ACAATCCAAA CAGAACCTGA GAAAACTGCG 
CTGTCAACAA AGCCATCAAA CTCTCAAAAT AATCAACTGG CTGAAGAGAA GCCAAAGGAA 
TCCAGTGGTG CAAAAAGCCC AATTGCACCT TCTGTGGTAA CAGCTTCTAA ACCAAATAAT 
ATGGGAAAGG AAACTACAAG TCAAGCTGAG CGGCCTTCAC AGGCAACCTC TGCAGACACA 
GCACAGCCTA AGACTGAAAA GACATCCACA GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT 
GCTGTCGCGA GTATGGTTGC TGCAGCTGAT AAACCAAATA GTGAGCCTGC TATGGATGAA 
TCAGCACTGG ACAGCTTAAT AGATACGCTA GGTGGATCTG AAGAAGATGT GGCTACTAGA 
CCAGTTTACA CTGGTCCGGA GATTACGGAA AATATTTCTT CAGAATACTT GGAAGAGCTG 
GGTAAACGAG AAGGTAGCCT CCCTCCAGAG TACCTTGCAT TATTGAAGAG CAAAGTGGAT 
GGCAAAGATG GAGGCCTACC AAAAGTAGAT GAACATTCTG AAAAACCGAT GACGGATGAT 
GAGCTTGCAG ATGCCCTGTC ATCTGACTTT ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG 
AAGACAACAG AGAAACAAAC TAAGGAAGGA GAAATCATAC AAGCACAAGT GACAAGTTCA 
GTGAAGACTT CAGTTCCTCC TAAAGAGAAG AAAACAAAAT CAAAAGAGGA AATCAAAGAA 
GATGCAATGG AAGCTCTCAT GGCTACTATT GGTGGACCTG AACCTGAACC CGAACCAAAA 
GATGTTAGCC CTATTGTAGA AGTGTCAGAG GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG 
GCTGGAGAAC GTGATGATAC AATACCTCCA GAATACAGGC TAACTCCAGA GCTGGATAAA 
GATGGAAAAC CAGTATTGCC AAAGCCTGAA GAAAAACCTA AGCCTTTGAG TGAATCTGAC 
CTTGTTGATG AATTTTCAAA AGACTTTGCC AGCCCTGCAC AGCCTGCAGT ACAATCCAAA 
CCATCAAAGC CCAGCAACAC ATCCAAAAAG CCTTCAGCTC CTGCATCTAC AAAAACTGCA 
AAGGATGAAG TAGTCCCTCG TGCCACAGCT TGCTCTGTGC AGTCTTCAGC TCCAACATCT 
GTGTCTTCTG TTGGTCACGT AGCTGATGAA GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA 
GAAAGGGAGC CTGATCCAGA AGAAAAGAAA CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC 
AAACGGAAAC AACACAAAAA ACTGGGTGAA GATGAAGAAA CAATTCCTCC AGAGTACAGA 
TTAACAGATG CAAAAGATAA AGATGGAAGA CCACTGTTAA TAAAACCTGA AGAGGAGTCC 
CAGCCTATGA GTGAAAACGA TCTGTTAGAA GGTTTGACAA AGGGATTTTC TCCAGCCCAA 
TCTGCACCAT TACCTACACA AACTGTAAAA AAGAAAACCA AAGGGGGTAA AAAAACTGCA 
GATTCTTCAG ATGTTATCTC TGCTTCTACA ATTTCCTCAG TGCACTCAGC AGCTCCCCTA 
GCTTCTACGT CGGGAGGAAA AGAGATGGAT GATGCCTTGG ATCTCTTGTC TGATTCCCTG 
GGACAGAGAG AACCTGACCC TGATGAGAAC AAACCAGTTG TGGATAAAGT AAAGGAAAAA 
GCTAAGTCTG AACACAGAGA CAAACTTGGA GAAAGAGATG ATACTATTCC ACCTGACTAT 
CGAAAACTCC TGGAGTCATC TGATGAGGGT AAACCTGTCA AGCCAACACC AAAGGATGCT 
TCGAAAAACA AAGAACAAAA GAAGCCTACA GATGAGTCTG CTGCTATTGA TGCCCTGTCA 
GGCGACTTTG ATACTAGTCC AAAGGCTCCT GCCACACCAC AGCACTCAAA GTGTAGGAGA 
AAAGTGGAAA GGAAACCGTC ACCACTAAAG CAACCCCAAA GGATGAAAGA AAACCCAAGG 
ATCATAAAAA GGCAAGGGGA CAGTCCTCCA GCAGCAAATC TGAGAAGCAG AAAACAAGCT 
AAACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_015280529.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136015.1 Gallus gallus calpastatin (CAST), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa48199
Clone ID Related Accession (Same CDS sequence)	XM_015280529.1 , XM_015280529.2
Accession Version	XM_015280529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCAT CTGAAGCAAA GTCCACACCA ACAATCCAAA CAGAACCTGA GAAAACTGCG 
CTGTCAACAA AGCCATCAAA CTCTCAAAAT AATCAACTGG CTGAAGAGAA GCCAAAGGAA 
TCCAGTGGTG CAAAAAGCCC AATTGCACCT TCTGTGGTAA CAGCTTCTAA ACCAAATAAT 
ATGGGAAAGG AAACTACAAG TCAAGCTGAG CGGCCTTCAC AGGCAACCTC TGCAGACACA 
GCACAGCCTA AGACTGAAAA GACATCCACA GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT 
GCTGTCGCGA GTATGGTTGC TGCAGCTGAT AAACCAAATA GTGAGCCTGC TATGGATGAA 
TCAGCACTGG ACAGCTTAAT AGATACGCTA GGTGGATCTG AAGAAGATGT GGCTACTAGA 
CCAGTTTACA CTGGTCCGGA GATTACGGAA AATATTTCTT CAGAATACTT GGAAGAGCTG 
GGTAAACGAG AAGGTAGCCT CCCTCCAGAG TACCTTGCAT TATTGAAGAG CAAAGTGGAT 
GGCAAAGATG GAGGCCTACC AAAAGTAGAT GAACATTCTG AAAAACCGAT GACGGATGAT 
GAGCTTGCAG ATGCCCTGTC ATCTGACTTT ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG 
AAGACAACAG AGAAACAAAC TAAGGAAGGA GAAATCATAC AAGCACAAGT GACAAGTTCA 
GTGAAGACTT CAGTTCCTCC TAAAGAGAAG AAAACAAAAT CAAAAGAGGA AATCAAAGAA 
GATGCAATGG AAGCTCTCAT GGCTACTATT GGTGGACCTG AACCTGAACC CGAACCAAAA 
GATGTTAGCC CTATTGTAGA AGTGTCAGAG GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG 
GCTGGAGAAC GTGATGATAC AATACCTCCA GAATACAGGC TAACTCCAGA GCTGGATAAA 
GATGGAAAAC CAGTATTGCC AAAGCCTGAA GAAAAACCTA AGCCTTTGAG TGAATCTGAC 
CTTGTTGATG AATTTTCAAA AGACTTTGCC AGCCCTGCAC AGCCTGCAGT ACAATCCAAA 
CCATCAAAGC CCAGCAACAC ATCCAAAAAG CCTTCAGCTC CTGCATCTAC AAAAACTGCA 
AAGGATGAAG TAGTCCCTCG TGCCACAGCT TGCTCTGTGC AGTCTTCAGC TCCAACATCT 
GTGTCTTCTG TTGGTCACGT AGCTGATGAA GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA 
GAAAGGGAGC CTGATCCAGA AGAAAAGAAA CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC 
AAACGGAAAC AACACAAAAA ACTGGGTGAA GATGAAGAAA CAATTCCTCC AGAGTACAGA 
TTAACAGATG CAAAAGATAA AGATGGAAGA CCACTGTTAA TAAAACCTGA AGAGGAGTCC 
CAGCCTATGA GTGAAAACGA TCTGTTAGAA GGTTTGACAA AGGGATTTTC TCCAGCCCAA 
TCTGCACCAT TACCTACACA AACTGTAAAA AAGAAAACCA AAGGGGGTAA AAAAACTGCA 
GATTCTTCAG ATGTTATCTC TGCTTCTACA ATTTCCTCAG TGCACTCAGC AGCTCCCCTA 
GCTTCTACGT CGGGAGGAAA AGAGATGGAT GATGCCTTGG ATCTCTTGTC TGATTCCCTG 
GGACAGAGAG AACCTGACCC TGATGAGAAC AAACCAGTTG TGGATAAAGT AAAGGAAAAA 
GCTAAGTCTG AACACAGAGA CAAACTTGGA GAAAGAGATG ATACTATTCC ACCTGACTAT 
CGAAAACTCC TGGAGTCATC TGATGAGGGT AAACCTGTCA AGCCAACACC AAAGGATGCT 
TCGAAAAACA AAGAACAAAA GAAGCCTACA GATGAGTCTG CTGCTATTGA TGCCCTGTCA 
GGCGACTTTG ATACTAGTCC AAAGGCTCCT GCCACACCAC AGCACTCAAA GTGTAGGAGA 
AAAGTGGAAA GGAAACCGTC ACCACTAAAG CAACCCCAAA GGATGAAAGA AAACCCAAGG 
ATCATAAAAA GGCAAGGGGA CAGTCCTCCA GCAGCAAATC TGAGAAGCAG AAAACAAGCT 
AAACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136015.1
CDS	165..2273
Translation	MKPSEAKSTPTIQTEPEKTALSTKPSNSQNNQLAEEKPKESSGAKSPIAPSVVTASKPNNMGKETTSQAERPSQATSADTAQPKTEKTSTAGGAGAAGAGAVASMVAAADKPNSEPAMDESALDSLIDTLGGSEEDVATRPVYTGPEITENISSEYLEELGKREGSLPPEYLALLKSKVDGKDGGLPKVDEHSEKPMTDDELADALSSDFTCSIAAAEEKKTTEKQTKEGEIIQAQVTSSVKTSVPPKEKKTKSKEEIKEDAMEALMATIGGPEPEPEPKDVSPIVEVSEAKAKEKKEKKAGERDDTIPPEYRLTPELDKDGKPVLPKPEEKPKPLSESDLVDEFSKDFASPAQPAVQSKPSKPSNTSKKPSAPASTKTAKDEVVPRATACSVQSSAPTSVSSVGHVADEAVEALSSSLGEREPDPEEKKPAVDKVKEKAKRKQHKKLGEDEETIPPEYRLTDAKDKDGRPLLIKPEEESQPMSENDLLEGLTKGFSPAQSAPLPTQTVKKKTKGGKKTADSSDVISASTISSVHSAAPLASTSGGKEMDDALDLLSDSLGQREPDPDENKPVVDKVKEKAKSEHRDKLGERDDTIPPDYRKLLESSDEGKPVKPTPKDASKNKEQKKPTDESAAIDALSGDFDTSPKAPATPQHSKCRRKVERKPSPLKQPQRMKENPRIIKRQGDSPPAANLRSRKQAKR

Target ORF information:

RefSeq Version	XM_015280529.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calpastatin (CAST), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280529.2

ORF Insert Sequence:

ATGAAGCCAT CTGAAGCAAA GTCCACACCA ACAATCCAAA CAGAACCTGA GAAAACTGCG 
CTGTCAACAA AGCCATCAAA CTCTCAAAAT AATCAACTGG CTGAAGAGAA GCCAAAGGAA 
TCCAGTGGTG CAAAAAGCCC AATTGCACCT TCTGTGGTAA CAGCTTCTAA ACCAAATAAT 
ATGGGAAAGG AAACTACAAG TCAAGCTGAG CGGCCTTCAC AGGCAACCTC TGCAGACACA 
GCACAGCCTA AGACTGAAAA GACATCCACA GCAGGTGGTG CAGGTGCAGC AGGTGCAGGT 
GCTGTCGCGA GTATGGTTGC TGCAGCTGAT AAACCAAATA GTGAGCCTGC TATGGATGAA 
TCAGCACTGG ACAGCTTAAT AGATACGCTA GGTGGATCTG AAGAAGATGT GGCTACTAGA 
CCAGTTTACA CTGGTCCGGA GATTACGGAA AATATTTCTT CAGAATACTT GGAAGAGCTG 
GGTAAACGAG AAGGTAGCCT CCCTCCAGAG TACCTTGCAT TATTGAAGAG CAAAGTGGAT 
GGCAAAGATG GAGGCCTACC AAAAGTAGAT GAACATTCTG AAAAACCGAT GACGGATGAT 
GAGCTTGCAG ATGCCCTGTC ATCTGACTTT ACCTGTAGCA TTGCTGCTGC TGAAGAAAAG 
AAGACAACAG AGAAACAAAC TAAGGAAGGA GAAATCATAC AAGCACAAGT GACAAGTTCA 
GTGAAGACTT CAGTTCCTCC TAAAGAGAAG AAAACAAAAT CAAAAGAGGA AATCAAAGAA 
GATGCAATGG AAGCTCTCAT GGCTACTATT GGTGGACCTG AACCTGAACC CGAACCAAAA 
GATGTTAGCC CTATTGTAGA AGTGTCAGAG GCAAAAGCTA AAGAGAAAAA AGAAAAGAAG 
GCTGGAGAAC GTGATGATAC AATACCTCCA GAATACAGGC TAACTCCAGA GCTGGATAAA 
GATGGAAAAC CAGTATTGCC AAAGCCTGAA GAAAAACCTA AGCCTTTGAG TGAATCTGAC 
CTTGTTGATG AATTTTCAAA AGACTTTGCC AGCCCTGCAC AGCCTGCAGT ACAATCCAAA 
CCATCAAAGC CCAGCAACAC ATCCAAAAAG CCTTCAGCTC CTGCATCTAC AAAAACTGCA 
AAGGATGAAG TAGTCCCTCG TGCCACAGCT TGCTCTGTGC AGTCTTCAGC TCCAACATCT 
GTGTCTTCTG TTGGTCACGT AGCTGATGAA GCAGTAGAAG CCTTGTCCAG TAGTCTGGGA 
GAAAGGGAGC CTGATCCAGA AGAAAAGAAA CCTGCTGTGG ACAAAGTTAA GGAGAAAGCC 
AAACGGAAAC AACACAAAAA ACTGGGTGAA GATGAAGAAA CAATTCCTCC AGAGTACAGA 
TTAACAGATG CAAAAGATAA AGATGGAAGA CCACTGTTAA TAAAACCTGA AGAGGAGTCC 
CAGCCTATGA GTGAAAACGA TCTGTTAGAA GGTTTGACAA AGGGATTTTC TCCAGCCCAA 
TCTGCACCAT TACCTACACA AACTGTAAAA AAGAAAACCA AAGGGGGTAA AAAAACTGCA 
GATTCTTCAG ATGTTATCTC TGCTTCTACA ATTTCCTCAG TGCACTCAGC AGCTCCCCTA 
GCTTCTACGT CGGGAGGAAA AGAGATGGAT GATGCCTTGG ATCTCTTGTC TGATTCCCTG 
GGACAGAGAG AACCTGACCC TGATGAGAAC AAACCAGTTG TGGATAAAGT AAAGGAAAAA 
GCTAAGTCTG AACACAGAGA CAAACTTGGA GAAAGAGATG ATACTATTCC ACCTGACTAT 
CGAAAACTCC TGGAGTCATC TGATGAGGGT AAACCTGTCA AGCCAACACC AAAGGATGCT 
TCGAAAAACA AAGAACAAAA GAAGCCTACA GATGAGTCTG CTGCTATTGA TGCCCTGTCA 
GGCGACTTTG ATACTAGTCC AAAGGCTCCT GCCACACCAC AGCACTCAAA GTGTAGGAGA 
AAAGTGGAAA GGAAACCGTC ACCACTAAAG CAACCCCAAA GGATGAAAGA AAACCCAAGG 
ATCATAAAAA GGCAAGGGGA CAGTCCTCCA GCAGCAAATC TGAGAAGCAG AAAACAAGCT 
AAACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CAST
Entrez Gene ID	427121
Full Name	calpastatin
General protein information	Preferred Names calpastatin Names calpastatin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	CAST	NP_001035905.1
	CAST	XP_001091928.2
Mus musculus (house mouse)	Cast	NP_033947.1
Rattus norvegicus (Norway rat)	Cast	NP_445747.2
Gallus gallus (chicken)	CAST	NP_001131122.1
Pan troglodytes (chimpanzee)	CAST	XP_518168.4
Canis lupus familiaris (dog)	CAST	XP_005618134.1
Bos taurus (cattle)	CAST	NP_001025489.1
Xenopus tropicalis (tropical clawed frog)	cast	NP_001120309.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CAST cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

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