Cast cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cast
Entrez Gene ID	25403
Full Name	calpastatin
General protein information	Preferred Names calpastatin Names calpastatin calpain inhibitor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q11
Summary	inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017590646.1	XP_017446135.1	calpastatin isoform X3
XM_017590647.1	XP_017446136.1	calpastatin isoform X4
NM_001033715.1	NP_001028887.1	calpastatin isoform b
NM_001033716.1	NP_001028888.1	calpastatin isoform c
XM_006231699.3	XP_006231761.1	calpastatin isoform X2
NM_053295.2	NP_445747.2	calpastatin isoform a
XM_006231698.3	XP_006231760.1	calpastatin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CAST	NP_001035905.1
	CAST	XP_001091928.2
Mus musculus (house mouse)	Cast	NP_033947.1
Rattus norvegicus (Norway rat)	Cast	NP_445747.2
Gallus gallus (chicken)	CAST	NP_001131122.1
Pan troglodytes (chimpanzee)	CAST	XP_518168.4
Canis lupus familiaris (dog)	CAST	XP_005618134.1
Bos taurus (cattle)	CAST	NP_001025489.1
Xenopus tropicalis (tropical clawed frog)	cast	NP_001120309.1

Gene Ontology
Bibliography

Related articles in PubMed

SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.
Mochida S, Hida Y, Tanifuji S, Hagiwara A, Hamada S, Abe M, Ma H, Yasumura M, Kitajima I, Sakimura K, Ohtsuka T
Cell reports16(11)2901-2913(2016 09)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages.
Huang Z, Hoffmann FW, Norton RL, Hashimoto AC, Hoffmann PR
The Journal of biological chemistry286(40)34830-8(2011 Oct)

Calpain activation promotes BACE1 expression, amyloid precursor protein processing, and amyloid plaque formation in a transgenic mouse model of Alzheimer disease.
Liang B, Duan BY, Zhou XP, Gong JX, Luo ZG
The Journal of biological chemistry285(36)27737-44(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

calpastatin protein and mRNA levels were low at early developmental time points and increased dramatically by postnatal day 30
Title: Calpain 1 and Calpastatin expression is developmentally regulated in rat brain.

Over-expression of calpastatin inhibits calpain activation and improves myocardial function in endotoxaemia.
Title: Over-expression of calpastatin inhibits calpain activation and attenuates myocardial dysfunction during endotoxaemia.

Role of the calpain-calpastatin system in the density-dependent growth arrest.
Title: Role of the calpain-calpastatin system in the density-dependent growth arrest.

3.0 A structure of Ca(2+)-bound m-calpain in complex with the first calpastatin repeat, both from rat, revealing the mechanism of exclusive specificity
Title: Concerted multi-pronged attack by calpastatin to occlude the catalytic cleft of heterodimeric calpains.

2.4-A-resolution crystal structure of the calcium-bound calpain 2 heterodimer bound by one of the four inhibitory domains of calpastatin
Title: Calcium-bound structure of calpain and its mechanism of inhibition by calpastatin.

The following Cast gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cast cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa59633	XM_017590647.1 Latest version!	Rattus norvegicus calpastatin (Cast), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa24286	XM_006231698.3 Latest version!	Rattus norvegicus calpastatin (Cast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa59632	XM_017590646.1 Latest version!	Rattus norvegicus calpastatin (Cast), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa08652	NM_001033715.1 Latest version!	Rattus norvegicus calpastatin (Cast), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa08657	NM_001033716.1 Latest version!	Rattus norvegicus calpastatin (Cast), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa09202	NM_053295.2 Latest version!	Rattus norvegicus calpastatin (Cast), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa24287	XM_006231699.3 Latest version!	Rattus norvegicus calpastatin (Cast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cast ( XM_017590647.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446136.1 Rattus norvegicus calpastatin (Cast), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa59633
Clone ID Related Accession (Same CDS sequence)	XM_017590647.1
Accession Version	XM_017590647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGT TTCTGTCTAC TACTTTCTGG GAAGGCTCAC CAACTGCAGT GTGGCATGAG 
AAGCTTCATG AGGGTGAACA TAAGGGAGCT GGAGAAACAA TCTCTGTCTT CCAGGACCAT 
GTGATATGCT TAGAAGAAAG GGAACATGGG TCCAAACATC ATGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAATGTGG TGAAGATGAG GACACAGTAC CTGCTGAGTA CAGGTTAAAA 
CCAGCCAAGG ATAAAGATGG AAAACCACTA TTGCCAGAAC CTGAAGAAAC ATCTAAGTGC 
CTGAGTGAGT CAGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGTCCAACC TACATATCAA 
GAAAAACCAT CTATGCCAGC TGCAAAAATA AAGAAGGGCG TAGTGCCAGA TGATGCTGTA 
GAAACCTTGG CTAGAAGCCT GGGGACAAGG AAAGAAGATC CAGAAGATGA AAAATCTCTG 
GTGGATAAAG TCAAGGAGAA AGCTAAAGAA GAAGATCATG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TCGACTAGAA ATAGTCAAGG ATAAGGATGG AAAACCGCTC 
CTGCCAAAAG AAGCCGAGGA ACAACTTCCA CCCTTAAGCG ATGACTTCCT TCTTGATGCA 
TTGTCTCAGG ACTTCTCCAG TCCTGCAAAC ATATTGTCTC TTGGTTTTGA AGATGCCAAA 
CTTTCTGCTG CTGTTTCTGA AACAGTTTCC CAGGTGCCTG CTCCAAGCAA CCACACAGCA 
GCCCCACCGC CTGGCACTGA GAGGAGAGAC AAAGAACTCG ATGATGCCTT GGATGAACTT 
TCTGACAGTC TTGGACAAAG ACAGCCTGAT CCAGATGAGA ACAAACCACT AGATGATAAA 
GTAAAGGAAA AAATCAAAGC AGAGCATAGC GAGAAACTGG GAGAAAGAGA CGACACCATC 
CCCCCTGAAT ATAGGCATCT CCTGGATAAT GATGGGAAGG ACAAACCAGA GAAGCCACTG 
ACAAAGAACA CAGAGAAGCC TGGTCAGGAC CAGGACCCCA TTGATGCCCT CTCAGAAGAC 
TTGGATAGCT GCCCCCCAAC TACAGAGACT TCACAGAATA CAACAAAGGA GAAGGGTAAG 
AAGACTTCAA GTTCCAAAGC ATCCAAGAAT GAAGAGAAAA CAAAGGATTC CTCCAAGAAG 
ACAGAGGAAG TGCCCAAGCC AAAGGTGGAT GAAGATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446136.1
CDS	1043..2287
Translation	MGQFLSTTFWEGSPTAVWHEKLHEGEHKGAGETISVFQDHVICLEEREHGSKHHEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT

Target ORF information:

RefSeq Version	XM_017590647.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpastatin (Cast), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590647.1

ORF Insert Sequence:

ATGGGCCAGT TTCTGTCTAC TACTTTCTGG GAAGGCTCAC CAACTGCAGT GTGGCATGAG 
AAGCTTCATG AGGGTGAACA TAAGGGAGCT GGAGAAACAA TCTCTGTCTT CCAGGACCAT 
GTGATATGCT TAGAAGAAAG GGAACATGGG TCCAAACATC ATGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAATGTGG TGAAGATGAG GACACAGTAC CTGCTGAGTA CAGGTTAAAA 
CCAGCCAAGG ATAAAGATGG AAAACCACTA TTGCCAGAAC CTGAAGAAAC ATCTAAGTGC 
CTGAGTGAGT CAGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGTCCAACC TACATATCAA 
GAAAAACCAT CTATGCCAGC TGCAAAAATA AAGAAGGGCG TAGTGCCAGA TGATGCTGTA 
GAAACCTTGG CTAGAAGCCT GGGGACAAGG AAAGAAGATC CAGAAGATGA AAAATCTCTG 
GTGGATAAAG TCAAGGAGAA AGCTAAAGAA GAAGATCATG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TCGACTAGAA ATAGTCAAGG ATAAGGATGG AAAACCGCTC 
CTGCCAAAAG AAGCCGAGGA ACAACTTCCA CCCTTAAGCG ATGACTTCCT TCTTGATGCA 
TTGTCTCAGG ACTTCTCCAG TCCTGCAAAC ATATTGTCTC TTGGTTTTGA AGATGCCAAA 
CTTTCTGCTG CTGTTTCTGA AACAGTTTCC CAGGTGCCTG CTCCAAGCAA CCACACAGCA 
GCCCCACCGC CTGGCACTGA GAGGAGAGAC AAAGAACTCG ATGATGCCTT GGATGAACTT 
TCTGACAGTC TTGGACAAAG ACAGCCTGAT CCAGATGAGA ACAAACCACT AGATGATAAA 
GTAAAGGAAA AAATCAAAGC AGAGCATAGC GAGAAACTGG GAGAAAGAGA CGACACCATC 
CCCCCTGAAT ATAGGCATCT CCTGGATAAT GATGGGAAGG ACAAACCAGA GAAGCCACTG 
ACAAAGAACA CAGAGAAGCC TGGTCAGGAC CAGGACCCCA TTGATGCCCT CTCAGAAGAC 
TTGGATAGCT GCCCCCCAAC TACAGAGACT TCACAGAATA CAACAAAGGA GAAGGGTAAG 
AAGACTTCAA GTTCCAAAGC ATCCAAGAAT GAAGAGAAAA CAAAGGATTC CTCCAAGAAG 
ACAGAGGAAG TGCCCAAGCC AAAGGTGGAT GAAGATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_006231698.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231760.1 Rattus norvegicus calpastatin (Cast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24286
Clone ID Related Accession (Same CDS sequence)	XM_006231698.3
Accession Version	XM_006231698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CAAACTGGTG GTACAAGACG CATGTTAATG AAAAGAATAT TGGATCATCT 
TCTAAACCAG CAGAAAAGAA AGGATCTGAT GAGGTGACAG CTTCCTCTGC TGCCACTGGC 
ACATCTCCCA GAATGAGTAC CACAGGAGCT AAGGCTGTGA AAATAGAGTC TGAGAAGTCA 
CAATCAAGTG AGCCACCTGT GATTCATGAG AAAAAGCCAA AAGGAAAGCC AAAGGAAGGG 
TCAGAGCCCC AAACTCTTCC TAAGCATGCA TCAGATACAG GAAGTAAGCA TGCTCACAAA 
GAAAAAGCAC TTTCCAGATC AAATGAGCAG ATAGTGTCAG AGAAATCATC AGAATCAAAG 
ACAAAATTTC AAGATGCTCC TTCTGCTGAT GGAGAGAGTG TGGCTGGAGG TGTGACTGTG 
GCCACTGCCT CAGACAAGGT GGTTGTTAAG AAAAAAGAGA AGAAATCATT AACACCTACT 
TTACCTATGG AGTCCACACT AAACAAGCTA TCTGACAAGT CAGGCGTGAA TGCTGCTTTG 
GATGACCTGA TAGACACCTT AGGTGAATGT GAAGATACTA ACAAAGATGA TCCACCATAT 
ACTGGACCAG TAGTTCTGGA CCCAATGGAT TCTACCTACC TTGAGGCACT GGGTATAAAA 
GAAGGGACTA TTCCTCCAGA GTATAGGAAA CTTCTGGAGA AAAATGAAGC TATCACAGGA 
CCTCTTCCAG ATTCTCCTAA ACCTATGGGA ATCGACCATG CTATAGATGC CTTGTCATCT 
GATTTCACCT GTAGCTCTCC AACTGGAAAG CAAACTGAGA AAGAGAAGTC TACTGGGGAA 
AGTTCGAAAG CTCAGTCTGC AGGAGTGACC AGAAGTGCTG TTCCTCCCCA GGAGAAGAAA 
AGGAAAGTAG AAGAGGAGGT CATGAATGAT CAAGCCCTGC AGGCTCTGTC AGATTCACTG 
GGCACCCGGC AGCCAGACCC TCAGAGCCAT CTTAGGCAAG CCAAACAAGT CAAAGAGGCA 
AAAGCAAAAG AAGAAAGGCA GGAGAAATGT GGTGAAGATG AGGACACAGT ACCTGCTGAG 
TACAGGTTAA AACCAGCCAA GGATAAAGAT GGAAAACCAC TATTGCCAGA ACCTGAAGAA 
ACATCTAAGT GCCTGAGTGA GTCAGAGCTG ATTGGGGAGC TTTCAGCAGA TTTTGTCCAA 
CCTACATATC AAGAAAAACC ATCTATGCCA GCTGCAAAAA TAAAGAAGGG CGTAGTGCCA 
GATGATGCTG TAGAAACCTT GGCTAGAAGC CTGGGGACAA GGAAAGAAGA TCCAGAAGAT 
GAAAAATCTC TGGTGGATAA AGTCAAGGAG AAAGCTAAAG AAGAAGATCA TGAAAAACTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATCGACTAG AAATAGTCAA GGATAAGGAT 
GGAAAACCGC TCCTGCCAAA AGAAGCCGAG GAACAACTTC CACCCTTAAG CGATGACTTC 
CTTCTTGATG CATTGTCTCA GGACTTCTCC AGTCCTGCAA ACATATTGTC TCTTGGTTTT 
GAAGATGCCA AACTTTCTGC TGCTGTTTCT GAAACAGTTT CCCAGGTGCC TGCTCCAAGC 
AACCACACAG CAGCCCCACC GCCTGGCACT GAGAGGAGAG ACAAAGAACT CGATGATGCC 
TTGGATGAAC TTTCTGACAG TCTTGGACAA AGACAGCCTG ATCCAGATGA GAACAAACCA 
CTAGATGATA AAGTAAAGGA AAAAATCAAA GCAGAGCATA GCGAGAAACT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGGCAT CTCCTGGATA ATGATGGGAA GGACAAACCA 
GAGAAGCCAC TGACAAAGAA CACAGAGAAG CCTGGTCAGG ACCAGGACCC CATTGATGCC 
CTCTCAGAAG ACTTGGATAG CTGCCCCCCA ACTACAGAGA CTTCACAGAA TACAACAAAG 
GAGAAGGGTA AGAAGACTTC AAGTTCCAAA GCATCCAAGA ATGAAGAGAA AACAAAGGAT 
TCCTCCAAGA AGACAGAGGA AGTGCCCAAG CCAAAGGTGG ATGAAGATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231760.1
CDS	595..2691
Translation	MAFANWWYKTHVNEKNIGSSSKPAEKKGSDEVTASSAATGTSPRMSTTGAKAVKIESEKSQSSEPPVIHEKKPKGKPKEGSEPQTLPKHASDTGSKHAHKEKALSRSNEQIVSEKSSESKTKFQDAPSADGESVAGGVTVATASDKVVVKKKEKKSLTPTLPMESTLNKLSDKSGVNAALDDLIDTLGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAIDALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGTRQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT

Target ORF information:

RefSeq Version	XM_006231698.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpastatin (Cast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231698.3

ORF Insert Sequence:

ATGGCATTCG CAAACTGGTG GTACAAGACG CATGTTAATG AAAAGAATAT TGGATCATCT 
TCTAAACCAG CAGAAAAGAA AGGATCTGAT GAGGTGACAG CTTCCTCTGC TGCCACTGGC 
ACATCTCCCA GAATGAGTAC CACAGGAGCT AAGGCTGTGA AAATAGAGTC TGAGAAGTCA 
CAATCAAGTG AGCCACCTGT GATTCATGAG AAAAAGCCAA AAGGAAAGCC AAAGGAAGGG 
TCAGAGCCCC AAACTCTTCC TAAGCATGCA TCAGATACAG GAAGTAAGCA TGCTCACAAA 
GAAAAAGCAC TTTCCAGATC AAATGAGCAG ATAGTGTCAG AGAAATCATC AGAATCAAAG 
ACAAAATTTC AAGATGCTCC TTCTGCTGAT GGAGAGAGTG TGGCTGGAGG TGTGACTGTG 
GCCACTGCCT CAGACAAGGT GGTTGTTAAG AAAAAAGAGA AGAAATCATT AACACCTACT 
TTACCTATGG AGTCCACACT AAACAAGCTA TCTGACAAGT CAGGCGTGAA TGCTGCTTTG 
GATGACCTGA TAGACACCTT AGGTGAATGT GAAGATACTA ACAAAGATGA TCCACCATAT 
ACTGGACCAG TAGTTCTGGA CCCAATGGAT TCTACCTACC TTGAGGCACT GGGTATAAAA 
GAAGGGACTA TTCCTCCAGA GTATAGGAAA CTTCTGGAGA AAAATGAAGC TATCACAGGA 
CCTCTTCCAG ATTCTCCTAA ACCTATGGGA ATCGACCATG CTATAGATGC CTTGTCATCT 
GATTTCACCT GTAGCTCTCC AACTGGAAAG CAAACTGAGA AAGAGAAGTC TACTGGGGAA 
AGTTCGAAAG CTCAGTCTGC AGGAGTGACC AGAAGTGCTG TTCCTCCCCA GGAGAAGAAA 
AGGAAAGTAG AAGAGGAGGT CATGAATGAT CAAGCCCTGC AGGCTCTGTC AGATTCACTG 
GGCACCCGGC AGCCAGACCC TCAGAGCCAT CTTAGGCAAG CCAAACAAGT CAAAGAGGCA 
AAAGCAAAAG AAGAAAGGCA GGAGAAATGT GGTGAAGATG AGGACACAGT ACCTGCTGAG 
TACAGGTTAA AACCAGCCAA GGATAAAGAT GGAAAACCAC TATTGCCAGA ACCTGAAGAA 
ACATCTAAGT GCCTGAGTGA GTCAGAGCTG ATTGGGGAGC TTTCAGCAGA TTTTGTCCAA 
CCTACATATC AAGAAAAACC ATCTATGCCA GCTGCAAAAA TAAAGAAGGG CGTAGTGCCA 
GATGATGCTG TAGAAACCTT GGCTAGAAGC CTGGGGACAA GGAAAGAAGA TCCAGAAGAT 
GAAAAATCTC TGGTGGATAA AGTCAAGGAG AAAGCTAAAG AAGAAGATCA TGAAAAACTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATCGACTAG AAATAGTCAA GGATAAGGAT 
GGAAAACCGC TCCTGCCAAA AGAAGCCGAG GAACAACTTC CACCCTTAAG CGATGACTTC 
CTTCTTGATG CATTGTCTCA GGACTTCTCC AGTCCTGCAA ACATATTGTC TCTTGGTTTT 
GAAGATGCCA AACTTTCTGC TGCTGTTTCT GAAACAGTTT CCCAGGTGCC TGCTCCAAGC 
AACCACACAG CAGCCCCACC GCCTGGCACT GAGAGGAGAG ACAAAGAACT CGATGATGCC 
TTGGATGAAC TTTCTGACAG TCTTGGACAA AGACAGCCTG ATCCAGATGA GAACAAACCA 
CTAGATGATA AAGTAAAGGA AAAAATCAAA GCAGAGCATA GCGAGAAACT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGGCAT CTCCTGGATA ATGATGGGAA GGACAAACCA 
GAGAAGCCAC TGACAAAGAA CACAGAGAAG CCTGGTCAGG ACCAGGACCC CATTGATGCC 
CTCTCAGAAG ACTTGGATAG CTGCCCCCCA ACTACAGAGA CTTCACAGAA TACAACAAAG 
GAGAAGGGTA AGAAGACTTC AAGTTCCAAA GCATCCAAGA ATGAAGAGAA AACAAAGGAT 
TCCTCCAAGA AGACAGAGGA AGTGCCCAAG CCAAAGGTGG ATGAAGATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_017590646.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446135.1 Rattus norvegicus calpastatin (Cast), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa59632
Clone ID Related Accession (Same CDS sequence)	XM_017590646.1
Accession Version	XM_017590646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpastatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACCA CAGGAGCTAA GGCTGTGAAA ATAGAGTCTG AGAAGTCACA ATCAAGTGAG 
CCACCTGTGA TTCATGAGAA AAAGCCAAAA GGAAAGCCAA AGGAAGGGTC AGAGCCCCAA 
ACTCTTCCTA AGCATGCATC AGATACAGGA AGTAAGCATG CTCACAAAGA AAAAGCACTT 
TCCAGATCAA ATGAGCAGAT AGTGTCAGAG AAATCATCAG AATCAAAGAC AAAATTTCAA 
GATGCTCCTT CTGCTGATGG AGAGAGTGTG GCTGGAGGTG TGACTGTGGC CACTGCCTCA 
GACAAGGTGG TTGTTAAGAA AAAAGAGAAG AAATCATTAA CACCTACTTT ACCTATGGAG 
TCCACACTAA ACAAGCTATC TGACAAGTCA GGCGTGAATG CTGCTTTGGA TGACCTGATA 
GACACCTTAG GTGAATGTGA AGATACTAAC AAAGATGATC CACCATATAC TGGACCAGTA 
GTTCTGGACC CAATGGATTC TACCTACCTT GAGGCACTGG GTATAAAAGA AGGGACTATT 
CCTCCAGAGT ATAGGAAACT TCTGGAGAAA AATGAAGCTA TCACAGGACC TCTTCCAGAT 
TCTCCTAAAC CTATGGGAAT CGACCATGCT ATAGATGCCT TGTCATCTGA TTTCACCTGT 
AGCTCTCCAA CTGGAAAGCA AACTGAGAAA GAGAAGTCTA CTGGGGAAAG TTCGAAAGCT 
CAGTCTGCAG GAGTGACCAG AAGTGCTGTT CCTCCCCAGG AGAAGAAAAG GAAAGTAGAA 
GAGGAGGTCA TGAATGATCA AGCCCTGCAG GCTCTGTCAG ATTCACTGGG CACCCGGCAG 
CCAGACCCTC AGAGCCATCT TAGGCAAGCC AAACAAGTCA AAGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAATGTGG TGAAGATGAG GACACAGTAC CTGCTGAGTA CAGGTTAAAA 
CCAGCCAAGG ATAAAGATGG AAAACCACTA TTGCCAGAAC CTGAAGAAAC ATCTAAGTGC 
CTGAGTGAGT CAGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGTCCAACC TACATATCAA 
GAAAAACCAT CTATGCCAGC TGCAAAAATA AAGAAGGGCG TAGTGCCAGA TGATGCTGTA 
GAAACCTTGG CTAGAAGCCT GGGGACAAGG AAAGAAGATC CAGAAGATGA AAAATCTCTG 
GTGGATAAAG TCAAGGAGAA AGCTAAAGAA GAAGATCATG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TCGACTAGAA ATAGTCAAGG ATAAGGATGG AAAACCGCTC 
CTGCCAAAAG AAGCCGAGGA ACAACTTCCA CCCTTAAGCG ATGACTTCCT TCTTGATGCA 
TTGTCTCAGG ACTTCTCCAG TCCTGCAAAC ATATTGTCTC TTGGTTTTGA AGATGCCAAA 
CTTTCTGCTG CTGTTTCTGA AACAGTTTCC CAGGTGCCTG CTCCAAGCAA CCACACAGCA 
GCCCCACCGC CTGGCACTGA GAGGAGAGAC AAAGAACTCG ATGATGCCTT GGATGAACTT 
TCTGACAGTC TTGGACAAAG ACAGCCTGAT CCAGATGAGA ACAAACCACT AGATGATAAA 
GTAAAGGAAA AAATCAAAGC AGAGCATAGC GAGAAACTGG GAGAAAGAGA CGACACCATC 
CCCCCTGAAT ATAGGCATCT CCTGGATAAT GATGGGAAGG ACAAACCAGA GAAGCCACTG 
ACAAAGAACA CAGAGAAGCC TGGTCAGGAC CAGGACCCCA TTGATGCCCT CTCAGAAGAC 
TTGGATAGCT GCCCCCCAAC TACAGAGACT TCACAGAATA CAACAAAGGA GAAGGGTAAG 
AAGACTTCAA GTTCCAAAGC ATCCAAGAAT GAAGAGAAAA CAAAGGATTC CTCCAAGAAG 
ACAGAGGAAG TGCCCAAGCC AAAGGTGGAT GAAGATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446135.1
CDS	1654..3618
Translation	MSTTGAKAVKIESEKSQSSEPPVIHEKKPKGKPKEGSEPQTLPKHASDTGSKHAHKEKALSRSNEQIVSEKSSESKTKFQDAPSADGESVAGGVTVATASDKVVVKKKEKKSLTPTLPMESTLNKLSDKSGVNAALDDLIDTLGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAIDALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGTRQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT

Target ORF information:

RefSeq Version	XM_017590646.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpastatin (Cast), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590646.1

ORF Insert Sequence:

ATGAGTACCA CAGGAGCTAA GGCTGTGAAA ATAGAGTCTG AGAAGTCACA ATCAAGTGAG 
CCACCTGTGA TTCATGAGAA AAAGCCAAAA GGAAAGCCAA AGGAAGGGTC AGAGCCCCAA 
ACTCTTCCTA AGCATGCATC AGATACAGGA AGTAAGCATG CTCACAAAGA AAAAGCACTT 
TCCAGATCAA ATGAGCAGAT AGTGTCAGAG AAATCATCAG AATCAAAGAC AAAATTTCAA 
GATGCTCCTT CTGCTGATGG AGAGAGTGTG GCTGGAGGTG TGACTGTGGC CACTGCCTCA 
GACAAGGTGG TTGTTAAGAA AAAAGAGAAG AAATCATTAA CACCTACTTT ACCTATGGAG 
TCCACACTAA ACAAGCTATC TGACAAGTCA GGCGTGAATG CTGCTTTGGA TGACCTGATA 
GACACCTTAG GTGAATGTGA AGATACTAAC AAAGATGATC CACCATATAC TGGACCAGTA 
GTTCTGGACC CAATGGATTC TACCTACCTT GAGGCACTGG GTATAAAAGA AGGGACTATT 
CCTCCAGAGT ATAGGAAACT TCTGGAGAAA AATGAAGCTA TCACAGGACC TCTTCCAGAT 
TCTCCTAAAC CTATGGGAAT CGACCATGCT ATAGATGCCT TGTCATCTGA TTTCACCTGT 
AGCTCTCCAA CTGGAAAGCA AACTGAGAAA GAGAAGTCTA CTGGGGAAAG TTCGAAAGCT 
CAGTCTGCAG GAGTGACCAG AAGTGCTGTT CCTCCCCAGG AGAAGAAAAG GAAAGTAGAA 
GAGGAGGTCA TGAATGATCA AGCCCTGCAG GCTCTGTCAG ATTCACTGGG CACCCGGCAG 
CCAGACCCTC AGAGCCATCT TAGGCAAGCC AAACAAGTCA AAGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAATGTGG TGAAGATGAG GACACAGTAC CTGCTGAGTA CAGGTTAAAA 
CCAGCCAAGG ATAAAGATGG AAAACCACTA TTGCCAGAAC CTGAAGAAAC ATCTAAGTGC 
CTGAGTGAGT CAGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGTCCAACC TACATATCAA 
GAAAAACCAT CTATGCCAGC TGCAAAAATA AAGAAGGGCG TAGTGCCAGA TGATGCTGTA 
GAAACCTTGG CTAGAAGCCT GGGGACAAGG AAAGAAGATC CAGAAGATGA AAAATCTCTG 
GTGGATAAAG TCAAGGAGAA AGCTAAAGAA GAAGATCATG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TCGACTAGAA ATAGTCAAGG ATAAGGATGG AAAACCGCTC 
CTGCCAAAAG AAGCCGAGGA ACAACTTCCA CCCTTAAGCG ATGACTTCCT TCTTGATGCA 
TTGTCTCAGG ACTTCTCCAG TCCTGCAAAC ATATTGTCTC TTGGTTTTGA AGATGCCAAA 
CTTTCTGCTG CTGTTTCTGA AACAGTTTCC CAGGTGCCTG CTCCAAGCAA CCACACAGCA 
GCCCCACCGC CTGGCACTGA GAGGAGAGAC AAAGAACTCG ATGATGCCTT GGATGAACTT 
TCTGACAGTC TTGGACAAAG ACAGCCTGAT CCAGATGAGA ACAAACCACT AGATGATAAA 
GTAAAGGAAA AAATCAAAGC AGAGCATAGC GAGAAACTGG GAGAAAGAGA CGACACCATC 
CCCCCTGAAT ATAGGCATCT CCTGGATAAT GATGGGAAGG ACAAACCAGA GAAGCCACTG 
ACAAAGAACA CAGAGAAGCC TGGTCAGGAC CAGGACCCCA TTGATGCCCT CTCAGAAGAC 
TTGGATAGCT GCCCCCCAAC TACAGAGACT TCACAGAATA CAACAAAGGA GAAGGGTAAG 
AAGACTTCAA GTTCCAAAGC ATCCAAGAAT GAAGAGAAAA CAAAGGATTC CTCCAAGAAG 
ACAGAGGAAG TGCCCAAGCC AAAGGTGGAT GAAGATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_001033715.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001028887.1 Rattus norvegicus calpastatin (Cast), transcript variant 2, mRNA.

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CloneID	ORa08652
Clone ID Related Accession (Same CDS sequence)	NM_001033715.1
Accession Version	NM_001033715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	calpastatin isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC091239.1. Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091239.1, DQ186627.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCCGGC CCGGCCCGAA GCCCGCCGCC TCTTCCAGGC CCCGGCGCGG GGCGGCAGCT 
AGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGCAGAA 
AAGAAAGGAT CTGATGAGGT GACAGCTTCC TCTGCTGCCA CTGGCACATC TCCCAGAATG 
AGTACCACAG GAGCTAAGGC TGTGAAAATA GAGTCTGAGA AGTCACAATC AAGTGAGCCA 
CCTGTGATTC ATGAGAAAAA GCCAAAAGGA AAGCCAAAGG AAGGGTCAGA GACAAAATTT 
CAAGATGCTC CTTCTGCTGA TGGAGAGAGT GTGGCTGGAG GTGTGACTGT GGCCACTGCC 
TCAGACAAGG TGGTTGTTAA GAAAAAAGAG AAGAAATCAT TAACACCTAC TTTACCTATG 
GAGTCCACAC TAAACAAGCT ATCTGACAAG TCAGGCGTGA ATGCTGCTTT GGATGACCTG 
ATAGACACCT TAGGTGAATG TGAAGATACT AACAAAGATG ATCCACCATA TACTGGACCA 
GTAGTTCTGG ACCCAATGGA TTCTACCTAC CTTGAGGCAC TGGGTATAAA AGAAGGGACT 
ATTCCTCCAG AGTATAGGAA ACTTCTGGAG AAAAATGAAG CTATCACAGG ACCTCTTCCA 
GATTCTCCTA AACCTATGGG AATCGACCAT GCTATAGATG CCTTGTCATC TGATTTCACC 
TGTAGCTCTC CAACTGGAAA GCAAACTGAG AAAGAGAAGT CTACTGGGGA AAGTTCGAAA 
GCTCAGTCTG CAGGAGTGAC CAGAAGTGCT GTTCCTCCCC AGGAGAAGAA AAGGAAAGTA 
GAAGAGGAGG TCATGAATGA TCAAGCCCTG CAGGCTCTGT CAGATTCACT GGGCACCCGG 
CAGCCAGACC CTCAGAGCCA TCTTAGGCAA GCCAAACAAG TCAAAGAGGC AAAAGCAAAA 
GAAGAAAGGC AGGAGAAATG TGGTGAAGAT GAGGACACAG TACCTGCTGA GTACAGGTTA 
AAACCAGCCA AGGATAAAGA TGGAAAACCA CTATTGCCAG AACCTGAAGA AACATCTAAG 
TGCCTGAGTG AGTCAGAGCT GATTGGGGAG CTTTCAGCAG ATTTTGTCCA ACCTACATAT 
CAAGAAAAAC CATCTATGCC AGCTGCAAAA ATAAAGAAGG GCGTAGTGCC AGATGATGCT 
GTAGAAACCT TGGCTAGAAG CCTGGGGACA AGGAAAGAAG ATCCAGAAGA TGAAAAATCT 
CTGGTGGATA AAGTCAAGGA GAAAGCTAAA GAAGAAGATC ATGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATCGACTA GAAATAGTCA AGGATAAGGA TGGAAAACCG 
CTCCTGCCAA AAGAAGCCGA GGAACAACTT CCACCCTTAA GCGATGACTT CCTTCTTGAT 
GCATTGTCTC AGGACTTCTC CAGTCCTGCA AACATATTGT CTCTTGGTTT TGAAGATGCC 
AAACTTTCTG CTGCTGTTTC TGAAACAGTT TCCCAGGTGC CTGCTCCAAG CAACCACACA 
GCAGCCCCAC CGCCTGGCAC TGAGAGGAGA GACAAAGAAC TCGATGATGC CTTGGATGAA 
CTTTCTGACA GTCTTGGACA AAGACAGCCT GATCCAGATG AGAACAAACC ACTAGATGAT 
AAAGTAAAGG AAAAAATCAA AGCAGAGCAT AGCGAGAAAC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGGCA TCTCCTGGAT AATGATGGGA AGGACAAACC AGAGAAGCCA 
CTGACAAAGA ACACAGAGAA GCCTGGTCAG GACCAGGACC CCATTGATGC CCTCTCAGAA 
GACTTGGATA GCTGCCCCCC AACTACAGAG ACTTCACAGA ATACAACAAA GGAGAAGGGT 
AAGAAGACTT CAAGTTCCAA AGCATCCAAG AATGAAGAGA AAACAAAGGA TTCCTCCAAG 
AAGACAGAGG AAGTGCCCAA GCCAAAGGTG GATGAAGATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028887.1
CDS	148..2175
Translation	MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGSDEVTASSAATGTSPRMSTTGAKAVKIESEKSQSSEPPVIHEKKPKGKPKEGSETKFQDAPSADGESVAGGVTVATASDKVVVKKKEKKSLTPTLPMESTLNKLSDKSGVNAALDDLIDTLGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAIDALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGTRQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT

Target ORF information:

RefSeq Version	NM_001033715.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpastatin (Cast), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033715.1

ORF Insert Sequence:

ATGTCCCGGC CCGGCCCGAA GCCCGCCGCC TCTTCCAGGC CCCGGCGCGG GGCGGCAGCT 
AGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGCAGAA 
AAGAAAGGAT CTGATGAGGT GACAGCTTCC TCTGCTGCCA CTGGCACATC TCCCAGAATG 
AGTACCACAG GAGCTAAGGC TGTGAAAATA GAGTCTGAGA AGTCACAATC AAGTGAGCCA 
CCTGTGATTC ATGAGAAAAA GCCAAAAGGA AAGCCAAAGG AAGGGTCAGA GACAAAATTT 
CAAGATGCTC CTTCTGCTGA TGGAGAGAGT GTGGCTGGAG GTGTGACTGT GGCCACTGCC 
TCAGACAAGG TGGTTGTTAA GAAAAAAGAG AAGAAATCAT TAACACCTAC TTTACCTATG 
GAGTCCACAC TAAACAAGCT ATCTGACAAG TCAGGCGTGA ATGCTGCTTT GGATGACCTG 
ATAGACACCT TAGGTGAATG TGAAGATACT AACAAAGATG ATCCACCATA TACTGGACCA 
GTAGTTCTGG ACCCAATGGA TTCTACCTAC CTTGAGGCAC TGGGTATAAA AGAAGGGACT 
ATTCCTCCAG AGTATAGGAA ACTTCTGGAG AAAAATGAAG CTATCACAGG ACCTCTTCCA 
GATTCTCCTA AACCTATGGG AATCGACCAT GCTATAGATG CCTTGTCATC TGATTTCACC 
TGTAGCTCTC CAACTGGAAA GCAAACTGAG AAAGAGAAGT CTACTGGGGA AAGTTCGAAA 
GCTCAGTCTG CAGGAGTGAC CAGAAGTGCT GTTCCTCCCC AGGAGAAGAA AAGGAAAGTA 
GAAGAGGAGG TCATGAATGA TCAAGCCCTG CAGGCTCTGT CAGATTCACT GGGCACCCGG 
CAGCCAGACC CTCAGAGCCA TCTTAGGCAA GCCAAACAAG TCAAAGAGGC AAAAGCAAAA 
GAAGAAAGGC AGGAGAAATG TGGTGAAGAT GAGGACACAG TACCTGCTGA GTACAGGTTA 
AAACCAGCCA AGGATAAAGA TGGAAAACCA CTATTGCCAG AACCTGAAGA AACATCTAAG 
TGCCTGAGTG AGTCAGAGCT GATTGGGGAG CTTTCAGCAG ATTTTGTCCA ACCTACATAT 
CAAGAAAAAC CATCTATGCC AGCTGCAAAA ATAAAGAAGG GCGTAGTGCC AGATGATGCT 
GTAGAAACCT TGGCTAGAAG CCTGGGGACA AGGAAAGAAG ATCCAGAAGA TGAAAAATCT 
CTGGTGGATA AAGTCAAGGA GAAAGCTAAA GAAGAAGATC ATGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATCGACTA GAAATAGTCA AGGATAAGGA TGGAAAACCG 
CTCCTGCCAA AAGAAGCCGA GGAACAACTT CCACCCTTAA GCGATGACTT CCTTCTTGAT 
GCATTGTCTC AGGACTTCTC CAGTCCTGCA AACATATTGT CTCTTGGTTT TGAAGATGCC 
AAACTTTCTG CTGCTGTTTC TGAAACAGTT TCCCAGGTGC CTGCTCCAAG CAACCACACA 
GCAGCCCCAC CGCCTGGCAC TGAGAGGAGA GACAAAGAAC TCGATGATGC CTTGGATGAA 
CTTTCTGACA GTCTTGGACA AAGACAGCCT GATCCAGATG AGAACAAACC ACTAGATGAT 
AAAGTAAAGG AAAAAATCAA AGCAGAGCAT AGCGAGAAAC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGGCA TCTCCTGGAT AATGATGGGA AGGACAAACC AGAGAAGCCA 
CTGACAAAGA ACACAGAGAA GCCTGGTCAG GACCAGGACC CCATTGATGC CCTCTCAGAA 
GACTTGGATA GCTGCCCCCC AACTACAGAG ACTTCACAGA ATACAACAAA GGAGAAGGGT 
AAGAAGACTT CAAGTTCCAA AGCATCCAAG AATGAAGAGA AAACAAAGGA TTCCTCCAAG 
AAGACAGAGG AAGTGCCCAA GCCAAAGGTG GATGAAGATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_001033716.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001028888.1 Rattus norvegicus calpastatin (Cast), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa08657
Clone ID Related Accession (Same CDS sequence)	NM_001033716.1
Accession Version	NM_001033716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	calpastatin isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC091239.1, AF346597.1 and Y13589.1. Transcript Variant: This variant (3) lacks two in-frame exons compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ186625.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCCGGC CCGGCCCGAA GCCCGCCGCC TCTTCCAGGC CCCGGCGCGG GGCGGCAGCT 
AGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGCAGAA 
AAGAAAGGAT CTGATGAGGT GACAGCTTCC TCTGCTGCCA CTGGCACATC TCCCAGAATG 
AGTACCACAG GAGCTAAGGC TGTGAAAATA GAGTCTGAGA AGTCACAATC AAGTGAGCCA 
CCTGTGATTC ATGAGAAAAA GCCAAAAGGA AAGCCAAAGG AAGGGTCAGA GACAAAATTT 
CAAGATGCTC CTTCTGCTGA TGGAGAGAGT GTGGCTGGAG GTGTGACTGT GGCCACTGCC 
TCAGACAAGG TGGTTGTTAA GTCAGGCGTG AATGCTGCTT TGGATGACCT GATAGACACC 
TTAGGTGAAT GTGAAGATAC TAACAAAGAT GATCCACCAT ATACTGGACC AGTAGTTCTG 
GACCCAATGG ATTCTACCTA CCTTGAGGCA CTGGGTATAA AAGAAGGGAC TATTCCTCCA 
GAGTATAGGA AACTTCTGGA GAAAAATGAA GCTATCACAG GACCTCTTCC AGATTCTCCT 
AAACCTATGG GAATCGACCA TGCTATAGAT GCCTTGTCAT CTGATTTCAC CTGTAGCTCT 
CCAACTGGAA AGCAAACTGA GAAAGAGAAG TCTACTGGGG AAAGTTCGAA AGCTCAGTCT 
GCAGGAGTGA CCAGAAGTGC TGTTCCTCCC CAGGAGAAGA AAAGGAAAGT AGAAGAGGAG 
GTCATGAATG ATCAAGCCCT GCAGGCTCTG TCAGATTCAC TGGGCACCCG GCAGCCAGAC 
CCTCAGAGCC ATCTTAGGCA AGCCAAACAA GTCAAAGAGG CAAAAGCAAA AGAAGAAAGG 
CAGGAGAAAT GTGGTGAAGA TGAGGACACA GTACCTGCTG AGTACAGGTT AAAACCAGCC 
AAGGATAAAG ATGGAAAACC ACTATTGCCA GAACCTGAAG AAACATCTAA GTGCCTGAGT 
GAGTCAGAGC TGATTGGGGA GCTTTCAGCA GATTTTGTCC AACCTACATA TCAAGAAAAA 
CCATCTATGC CAGCTGCAAA AATAAAGAAG GGCGTAGTGC CAGATGATGC TGTAGAAACC 
TTGGCTAGAA GCCTGGGGAC AAGGAAAGAA GATCCAGAAG ATGAAAAATC TCTGGTGGAT 
AAAGTCAAGG AGAAAGCTAA AGAAGAAGAT CATGAAAAAC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATCGACT AGAAATAGTC AAGGATAAGG ATGGAAAACC GCTCCTGCCA 
AAAGAAGCCG AGGAACAACT TCCACCCTTA AGCGATGACT TCCTTCTTGA TGCATTGTCT 
CAGGACTTCT CCAGTCCTGC AAACATATTG TCTCTTGGTT TTGAAGATGC CAAACTTTCT 
GCTGCTGTTT CTGAAACAGT TTCCCAGGTG CCTGCTCCAA GCAACCACAC AGCAGCCCCA 
CCGCCTGGCA CTGAGAGGAG AGACAAAGAA CTCGATGATG CCTTGGATGA ACTTTCTGAC 
AGTCTTGGAC AAAGACAGCC TGATCCAGAT GAGAACAAAC CACTAGATGA TAAAGTAAAG 
GAAAAAATCA AAGCAGAGCA TAGCGAGAAA CTGGGAGAAA GAGACGACAC CATCCCCCCT 
GAATATAGGC ATCTCCTGGA TAATGATGGG AAGGACAAAC CAGAGAAGCC ACTGACAAAG 
AACACAGAGA AGCCTGGTCA GGACCAGGAC CCCATTGATG CCCTCTCAGA AGACTTGGAT 
AGCTGCCCCC CAACTACAGA GACTTCACAG AATACAACAA AGGAGAAGGG TAAGAAGACT 
TCAAGTTCCA AAGCATCCAA GAATGAAGAG AAAACAAAGG ATTCCTCCAA GAAGACAGAG 
GAAGTGCCCA AGCCAAAGGT GGATGAAGAT GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028888.1
CDS	148..2106
Translation	MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGSDEVTASSAATGTSPRMSTTGAKAVKIESEKSQSSEPPVIHEKKPKGKPKEGSETKFQDAPSADGESVAGGVTVATASDKVVVKSGVNAALDDLIDTLGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAIDALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGTRQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT

Target ORF information:

RefSeq Version	NM_001033716.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpastatin (Cast), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033716.1

ORF Insert Sequence:

ATGTCCCGGC CCGGCCCGAA GCCCGCCGCC TCTTCCAGGC CCCGGCGCGG GGCGGCAGCT 
AGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGCAGAA 
AAGAAAGGAT CTGATGAGGT GACAGCTTCC TCTGCTGCCA CTGGCACATC TCCCAGAATG 
AGTACCACAG GAGCTAAGGC TGTGAAAATA GAGTCTGAGA AGTCACAATC AAGTGAGCCA 
CCTGTGATTC ATGAGAAAAA GCCAAAAGGA AAGCCAAAGG AAGGGTCAGA GACAAAATTT 
CAAGATGCTC CTTCTGCTGA TGGAGAGAGT GTGGCTGGAG GTGTGACTGT GGCCACTGCC 
TCAGACAAGG TGGTTGTTAA GTCAGGCGTG AATGCTGCTT TGGATGACCT GATAGACACC 
TTAGGTGAAT GTGAAGATAC TAACAAAGAT GATCCACCAT ATACTGGACC AGTAGTTCTG 
GACCCAATGG ATTCTACCTA CCTTGAGGCA CTGGGTATAA AAGAAGGGAC TATTCCTCCA 
GAGTATAGGA AACTTCTGGA GAAAAATGAA GCTATCACAG GACCTCTTCC AGATTCTCCT 
AAACCTATGG GAATCGACCA TGCTATAGAT GCCTTGTCAT CTGATTTCAC CTGTAGCTCT 
CCAACTGGAA AGCAAACTGA GAAAGAGAAG TCTACTGGGG AAAGTTCGAA AGCTCAGTCT 
GCAGGAGTGA CCAGAAGTGC TGTTCCTCCC CAGGAGAAGA AAAGGAAAGT AGAAGAGGAG 
GTCATGAATG ATCAAGCCCT GCAGGCTCTG TCAGATTCAC TGGGCACCCG GCAGCCAGAC 
CCTCAGAGCC ATCTTAGGCA AGCCAAACAA GTCAAAGAGG CAAAAGCAAA AGAAGAAAGG 
CAGGAGAAAT GTGGTGAAGA TGAGGACACA GTACCTGCTG AGTACAGGTT AAAACCAGCC 
AAGGATAAAG ATGGAAAACC ACTATTGCCA GAACCTGAAG AAACATCTAA GTGCCTGAGT 
GAGTCAGAGC TGATTGGGGA GCTTTCAGCA GATTTTGTCC AACCTACATA TCAAGAAAAA 
CCATCTATGC CAGCTGCAAA AATAAAGAAG GGCGTAGTGC CAGATGATGC TGTAGAAACC 
TTGGCTAGAA GCCTGGGGAC AAGGAAAGAA GATCCAGAAG ATGAAAAATC TCTGGTGGAT 
AAAGTCAAGG AGAAAGCTAA AGAAGAAGAT CATGAAAAAC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATCGACT AGAAATAGTC AAGGATAAGG ATGGAAAACC GCTCCTGCCA 
AAAGAAGCCG AGGAACAACT TCCACCCTTA AGCGATGACT TCCTTCTTGA TGCATTGTCT 
CAGGACTTCT CCAGTCCTGC AAACATATTG TCTCTTGGTT TTGAAGATGC CAAACTTTCT 
GCTGCTGTTT CTGAAACAGT TTCCCAGGTG CCTGCTCCAA GCAACCACAC AGCAGCCCCA 
CCGCCTGGCA CTGAGAGGAG AGACAAAGAA CTCGATGATG CCTTGGATGA ACTTTCTGAC 
AGTCTTGGAC AAAGACAGCC TGATCCAGAT GAGAACAAAC CACTAGATGA TAAAGTAAAG 
GAAAAAATCA AAGCAGAGCA TAGCGAGAAA CTGGGAGAAA GAGACGACAC CATCCCCCCT 
GAATATAGGC ATCTCCTGGA TAATGATGGG AAGGACAAAC CAGAGAAGCC ACTGACAAAG 
AACACAGAGA AGCCTGGTCA GGACCAGGAC CCCATTGATG CCCTCTCAGA AGACTTGGAT 
AGCTGCCCCC CAACTACAGA GACTTCACAG AATACAACAA AGGAGAAGGG TAAGAAGACT 
TCAAGTTCCA AAGCATCCAA GAATGAAGAG AAAACAAAGG ATTCCTCCAA GAAGACAGAG 
GAAGTGCCCA AGCCAAAGGT GGATGAAGAT GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_053295.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445747.2 Rattus norvegicus calpastatin (Cast), transcript variant 1, mRNA.

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CloneID	ORa09202
Clone ID Related Accession (Same CDS sequence)	NM_053295.2
Accession Version	NM_053295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	calpastatin isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC091239.1 and Y13588.1. On Sep 23, 2005 this sequence version replaced NM_053295.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ186624.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCCGGC CCGGCCCGAA GCCCGCCGCC TCTTCCAGGC CCCGGCGCGG GGCGGCAGCT 
AGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGCAGAA 
AAGAAAGGAT CTGATGAGGT GACAGCTTCC TCTGCTGCCA CTGGCACATC TCCCAGAATG 
AGTACCACAG GAGCTAAGGC TGTGAAAATA GAGTCTGAGA AGTCACAATC AAGTGAGCCA 
CCTGTGATTC ATGAGAAAAA GCCAAAAGGA AAGCCAAAGG AAGGGTCAGA GCCCCAAACT 
CTTCCTAAGC ATGCATCAGA TACAGGAAGT AAGCATGCTC ACAAAGAAAA AGCACTTTCC 
AGATCAAATG AGCAGATAGT GTCAGAGAAA TCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GCTCCTTCTG CTGATGGAGA GAGTGTGGCT GGAGGTGTGA CTGTGGCCAC TGCCTCAGAC 
AAGGTGGTTG TTAAGAAAAA AGAGAAGAAA TCATTAACAC CTACTTTACC TATGGAGTCC 
ACACTAAACA AGCTATCTGA CAAGTCAGGC GTGAATGCTG CTTTGGATGA CCTGATAGAC 
ACCTTAGGTG AATGTGAAGA TACTAACAAA GATGATCCAC CATATACTGG ACCAGTAGTT 
CTGGACCCAA TGGATTCTAC CTACCTTGAG GCACTGGGTA TAAAAGAAGG GACTATTCCT 
CCAGAGTATA GGAAACTTCT GGAGAAAAAT GAAGCTATCA CAGGACCTCT TCCAGATTCT 
CCTAAACCTA TGGGAATCGA CCATGCTATA GATGCCTTGT CATCTGATTT CACCTGTAGC 
TCTCCAACTG GAAAGCAAAC TGAGAAAGAG AAGTCTACTG GGGAAAGTTC GAAAGCTCAG 
TCTGCAGGAG TGACCAGAAG TGCTGTTCCT CCCCAGGAGA AGAAAAGGAA AGTAGAAGAG 
GAGGTCATGA ATGATCAAGC CCTGCAGGCT CTGTCAGATT CACTGGGCAC CCGGCAGCCA 
GACCCTCAGA GCCATCTTAG GCAAGCCAAA CAAGTCAAAG AGGCAAAAGC AAAAGAAGAA 
AGGCAGGAGA AATGTGGTGA AGATGAGGAC ACAGTACCTG CTGAGTACAG GTTAAAACCA 
GCCAAGGATA AAGATGGAAA ACCACTATTG CCAGAACCTG AAGAAACATC TAAGTGCCTG 
AGTGAGTCAG AGCTGATTGG GGAGCTTTCA GCAGATTTTG TCCAACCTAC ATATCAAGAA 
AAACCATCTA TGCCAGCTGC AAAAATAAAG AAGGGCGTAG TGCCAGATGA TGCTGTAGAA 
ACCTTGGCTA GAAGCCTGGG GACAAGGAAA GAAGATCCAG AAGATGAAAA ATCTCTGGTG 
GATAAAGTCA AGGAGAAAGC TAAAGAAGAA GATCATGAAA AACTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATCG ACTAGAAATA GTCAAGGATA AGGATGGAAA ACCGCTCCTG 
CCAAAAGAAG CCGAGGAACA ACTTCCACCC TTAAGCGATG ACTTCCTTCT TGATGCATTG 
TCTCAGGACT TCTCCAGTCC TGCAAACATA TTGTCTCTTG GTTTTGAAGA TGCCAAACTT 
TCTGCTGCTG TTTCTGAAAC AGTTTCCCAG GTGCCTGCTC CAAGCAACCA CACAGCAGCC 
CCACCGCCTG GCACTGAGAG GAGAGACAAA GAACTCGATG ATGCCTTGGA TGAACTTTCT 
GACAGTCTTG GACAAAGACA GCCTGATCCA GATGAGAACA AACCACTAGA TGATAAAGTA 
AAGGAAAAAA TCAAAGCAGA GCATAGCGAG AAACTGGGAG AAAGAGACGA CACCATCCCC 
CCTGAATATA GGCATCTCCT GGATAATGAT GGGAAGGACA AACCAGAGAA GCCACTGACA 
AAGAACACAG AGAAGCCTGG TCAGGACCAG GACCCCATTG ATGCCCTCTC AGAAGACTTG 
GATAGCTGCC CCCCAACTAC AGAGACTTCA CAGAATACAA CAAAGGAGAA GGGTAAGAAG 
ACTTCAAGTT CCAAAGCATC CAAGAATGAA GAGAAAACAA AGGATTCCTC CAAGAAGACA 
GAGGAAGTGC CCAAGCCAAA GGTGGATGAA GATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445747.2
CDS	148..2289
Translation	MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGSDEVTASSAATGTSPRMSTTGAKAVKIESEKSQSSEPPVIHEKKPKGKPKEGSEPQTLPKHASDTGSKHAHKEKALSRSNEQIVSEKSSESKTKFQDAPSADGESVAGGVTVATASDKVVVKKKEKKSLTPTLPMESTLNKLSDKSGVNAALDDLIDTLGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAIDALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGTRQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT

Target ORF information:

RefSeq Version	NM_053295.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpastatin (Cast), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053295.2

ORF Insert Sequence:

ATGTCCCGGC CCGGCCCGAA GCCCGCCGCC TCTTCCAGGC CCCGGCGCGG GGCGGCAGCT 
AGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGCAGAA 
AAGAAAGGAT CTGATGAGGT GACAGCTTCC TCTGCTGCCA CTGGCACATC TCCCAGAATG 
AGTACCACAG GAGCTAAGGC TGTGAAAATA GAGTCTGAGA AGTCACAATC AAGTGAGCCA 
CCTGTGATTC ATGAGAAAAA GCCAAAAGGA AAGCCAAAGG AAGGGTCAGA GCCCCAAACT 
CTTCCTAAGC ATGCATCAGA TACAGGAAGT AAGCATGCTC ACAAAGAAAA AGCACTTTCC 
AGATCAAATG AGCAGATAGT GTCAGAGAAA TCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GCTCCTTCTG CTGATGGAGA GAGTGTGGCT GGAGGTGTGA CTGTGGCCAC TGCCTCAGAC 
AAGGTGGTTG TTAAGAAAAA AGAGAAGAAA TCATTAACAC CTACTTTACC TATGGAGTCC 
ACACTAAACA AGCTATCTGA CAAGTCAGGC GTGAATGCTG CTTTGGATGA CCTGATAGAC 
ACCTTAGGTG AATGTGAAGA TACTAACAAA GATGATCCAC CATATACTGG ACCAGTAGTT 
CTGGACCCAA TGGATTCTAC CTACCTTGAG GCACTGGGTA TAAAAGAAGG GACTATTCCT 
CCAGAGTATA GGAAACTTCT GGAGAAAAAT GAAGCTATCA CAGGACCTCT TCCAGATTCT 
CCTAAACCTA TGGGAATCGA CCATGCTATA GATGCCTTGT CATCTGATTT CACCTGTAGC 
TCTCCAACTG GAAAGCAAAC TGAGAAAGAG AAGTCTACTG GGGAAAGTTC GAAAGCTCAG 
TCTGCAGGAG TGACCAGAAG TGCTGTTCCT CCCCAGGAGA AGAAAAGGAA AGTAGAAGAG 
GAGGTCATGA ATGATCAAGC CCTGCAGGCT CTGTCAGATT CACTGGGCAC CCGGCAGCCA 
GACCCTCAGA GCCATCTTAG GCAAGCCAAA CAAGTCAAAG AGGCAAAAGC AAAAGAAGAA 
AGGCAGGAGA AATGTGGTGA AGATGAGGAC ACAGTACCTG CTGAGTACAG GTTAAAACCA 
GCCAAGGATA AAGATGGAAA ACCACTATTG CCAGAACCTG AAGAAACATC TAAGTGCCTG 
AGTGAGTCAG AGCTGATTGG GGAGCTTTCA GCAGATTTTG TCCAACCTAC ATATCAAGAA 
AAACCATCTA TGCCAGCTGC AAAAATAAAG AAGGGCGTAG TGCCAGATGA TGCTGTAGAA 
ACCTTGGCTA GAAGCCTGGG GACAAGGAAA GAAGATCCAG AAGATGAAAA ATCTCTGGTG 
GATAAAGTCA AGGAGAAAGC TAAAGAAGAA GATCATGAAA AACTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATCG ACTAGAAATA GTCAAGGATA AGGATGGAAA ACCGCTCCTG 
CCAAAAGAAG CCGAGGAACA ACTTCCACCC TTAAGCGATG ACTTCCTTCT TGATGCATTG 
TCTCAGGACT TCTCCAGTCC TGCAAACATA TTGTCTCTTG GTTTTGAAGA TGCCAAACTT 
TCTGCTGCTG TTTCTGAAAC AGTTTCCCAG GTGCCTGCTC CAAGCAACCA CACAGCAGCC 
CCACCGCCTG GCACTGAGAG GAGAGACAAA GAACTCGATG ATGCCTTGGA TGAACTTTCT 
GACAGTCTTG GACAAAGACA GCCTGATCCA GATGAGAACA AACCACTAGA TGATAAAGTA 
AAGGAAAAAA TCAAAGCAGA GCATAGCGAG AAACTGGGAG AAAGAGACGA CACCATCCCC 
CCTGAATATA GGCATCTCCT GGATAATGAT GGGAAGGACA AACCAGAGAA GCCACTGACA 
AAGAACACAG AGAAGCCTGG TCAGGACCAG GACCCCATTG ATGCCCTCTC AGAAGACTTG 
GATAGCTGCC CCCCAACTAC AGAGACTTCA CAGAATACAA CAAAGGAGAA GGGTAAGAAG 
ACTTCAAGTT CCAAAGCATC CAAGAATGAA GAGAAAACAA AGGATTCCTC CAAGAAGACA 
GAGGAAGTGC CCAAGCCAAA GGTGGATGAA GATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_006231699.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231761.1 Rattus norvegicus calpastatin (Cast), transcript variant X2, mRNA.

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CloneID	ORa24287
Clone ID Related Accession (Same CDS sequence)	XM_006231699.3
Accession Version	XM_006231699.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGC CCGGCCCGAA GCCCGCCGCC TCTTCCAGGC CCCGGCGCGG GGCGGCAGCT 
AGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGCAGAA 
AAGAAAGGAT CTGATGAGGT GACAGCTTCC TCTGCTGCCA CTGGCACATC TCCCAGAATG 
AGTACCACAG GAGCTAAGGC TGTGAAAATA GAGTCTGAGA AGTCACAATC AAGTGAGCCA 
CCTGTGATTC ATGAGAAAAA GCCAAAAGGA AAGCCAAAGG AAGGGTCAGA GCCCCAAACT 
CTTCCTAAGC ATGCATCAGA TACAGGAAGT AAGCATGCTC ACAAAGAAAA AGCACTTTCC 
AGATCAAATG AGCAGATAGT GTCAGAGAAA TCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GCTCCTTCTG CTGATGGAGA GAGTGTGGCT GGAGGTGTGA CTGTGGCCAC TGCCTCAGAC 
AAGGTGGTTG TTAAGTCAGG CGTGAATGCT GCTTTGGATG ACCTGATAGA CACCTTAGGT 
GAATGTGAAG ATACTAACAA AGATGATCCA CCATATACTG GACCAGTAGT TCTGGACCCA 
ATGGATTCTA CCTACCTTGA GGCACTGGGT ATAAAAGAAG GGACTATTCC TCCAGAGTAT 
AGGAAACTTC TGGAGAAAAA TGAAGCTATC ACAGGACCTC TTCCAGATTC TCCTAAACCT 
ATGGGAATCG ACCATGCTAT AGATGCCTTG TCATCTGATT TCACCTGTAG CTCTCCAACT 
GGAAAGCAAA CTGAGAAAGA GAAGTCTACT GGGGAAAGTT CGAAAGCTCA GTCTGCAGGA 
GTGACCAGAA GTGCTGTTCC TCCCCAGGAG AAGAAAAGGA AAGTAGAAGA GGAGGTCATG 
AATGATCAAG CCCTGCAGGC TCTGTCAGAT TCACTGGGCA CCCGGCAGCC AGACCCTCAG 
AGCCATCTTA GGCAAGCCAA ACAAGTCAAA GAGGCAAAAG CAAAAGAAGA AAGGCAGGAG 
AAATGTGGTG AAGATGAGGA CACAGTACCT GCTGAGTACA GGTTAAAACC AGCCAAGGAT 
AAAGATGGAA AACCACTATT GCCAGAACCT GAAGAAACAT CTAAGTGCCT GAGTGAGTCA 
GAGCTGATTG GGGAGCTTTC AGCAGATTTT GTCCAACCTA CATATCAAGA AAAACCATCT 
ATGCCAGCTG CAAAAATAAA GAAGGGCGTA GTGCCAGATG ATGCTGTAGA AACCTTGGCT 
AGAAGCCTGG GGACAAGGAA AGAAGATCCA GAAGATGAAA AATCTCTGGT GGATAAAGTC 
AAGGAGAAAG CTAAAGAAGA AGATCATGAA AAACTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATC GACTAGAAAT AGTCAAGGAT AAGGATGGAA AACCGCTCCT GCCAAAAGAA 
GCCGAGGAAC AACTTCCACC CTTAAGCGAT GACTTCCTTC TTGATGCATT GTCTCAGGAC 
TTCTCCAGTC CTGCAAACAT ATTGTCTCTT GGTTTTGAAG ATGCCAAACT TTCTGCTGCT 
GTTTCTGAAA CAGTTTCCCA GGTGCCTGCT CCAAGCAACC ACACAGCAGC CCCACCGCCT 
GGCACTGAGA GGAGAGACAA AGAACTCGAT GATGCCTTGG ATGAACTTTC TGACAGTCTT 
GGACAAAGAC AGCCTGATCC AGATGAGAAC AAACCACTAG ATGATAAAGT AAAGGAAAAA 
ATCAAAGCAG AGCATAGCGA GAAACTGGGA GAAAGAGACG ACACCATCCC CCCTGAATAT 
AGGCATCTCC TGGATAATGA TGGGAAGGAC AAACCAGAGA AGCCACTGAC AAAGAACACA 
GAGAAGCCTG GTCAGGACCA GGACCCCATT GATGCCCTCT CAGAAGACTT GGATAGCTGC 
CCCCCAACTA CAGAGACTTC ACAGAATACA ACAAAGGAGA AGGGTAAGAA GACTTCAAGT 
TCCAAAGCAT CCAAGAATGA AGAGAAAACA AAGGATTCCT CCAAGAAGAC AGAGGAAGTG 
CCCAAGCCAA AGGTGGATGA AGATGCAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231761.1
CDS	353..2425
Translation	MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGSDEVTASSAATGTSPRMSTTGAKAVKIESEKSQSSEPPVIHEKKPKGKPKEGSEPQTLPKHASDTGSKHAHKEKALSRSNEQIVSEKSSESKTKFQDAPSADGESVAGGVTVATASDKVVVKSGVNAALDDLIDTLGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAIDALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGTRQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT

Target ORF information:

RefSeq Version	XM_006231699.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpastatin (Cast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231699.3

ORF Insert Sequence:

ATGTCCCGGC CCGGCCCGAA GCCCGCCGCC TCTTCCAGGC CCCGGCGCGG GGCGGCAGCT 
AGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGCAGAA 
AAGAAAGGAT CTGATGAGGT GACAGCTTCC TCTGCTGCCA CTGGCACATC TCCCAGAATG 
AGTACCACAG GAGCTAAGGC TGTGAAAATA GAGTCTGAGA AGTCACAATC AAGTGAGCCA 
CCTGTGATTC ATGAGAAAAA GCCAAAAGGA AAGCCAAAGG AAGGGTCAGA GCCCCAAACT 
CTTCCTAAGC ATGCATCAGA TACAGGAAGT AAGCATGCTC ACAAAGAAAA AGCACTTTCC 
AGATCAAATG AGCAGATAGT GTCAGAGAAA TCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GCTCCTTCTG CTGATGGAGA GAGTGTGGCT GGAGGTGTGA CTGTGGCCAC TGCCTCAGAC 
AAGGTGGTTG TTAAGTCAGG CGTGAATGCT GCTTTGGATG ACCTGATAGA CACCTTAGGT 
GAATGTGAAG ATACTAACAA AGATGATCCA CCATATACTG GACCAGTAGT TCTGGACCCA 
ATGGATTCTA CCTACCTTGA GGCACTGGGT ATAAAAGAAG GGACTATTCC TCCAGAGTAT 
AGGAAACTTC TGGAGAAAAA TGAAGCTATC ACAGGACCTC TTCCAGATTC TCCTAAACCT 
ATGGGAATCG ACCATGCTAT AGATGCCTTG TCATCTGATT TCACCTGTAG CTCTCCAACT 
GGAAAGCAAA CTGAGAAAGA GAAGTCTACT GGGGAAAGTT CGAAAGCTCA GTCTGCAGGA 
GTGACCAGAA GTGCTGTTCC TCCCCAGGAG AAGAAAAGGA AAGTAGAAGA GGAGGTCATG 
AATGATCAAG CCCTGCAGGC TCTGTCAGAT TCACTGGGCA CCCGGCAGCC AGACCCTCAG 
AGCCATCTTA GGCAAGCCAA ACAAGTCAAA GAGGCAAAAG CAAAAGAAGA AAGGCAGGAG 
AAATGTGGTG AAGATGAGGA CACAGTACCT GCTGAGTACA GGTTAAAACC AGCCAAGGAT 
AAAGATGGAA AACCACTATT GCCAGAACCT GAAGAAACAT CTAAGTGCCT GAGTGAGTCA 
GAGCTGATTG GGGAGCTTTC AGCAGATTTT GTCCAACCTA CATATCAAGA AAAACCATCT 
ATGCCAGCTG CAAAAATAAA GAAGGGCGTA GTGCCAGATG ATGCTGTAGA AACCTTGGCT 
AGAAGCCTGG GGACAAGGAA AGAAGATCCA GAAGATGAAA AATCTCTGGT GGATAAAGTC 
AAGGAGAAAG CTAAAGAAGA AGATCATGAA AAACTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATC GACTAGAAAT AGTCAAGGAT AAGGATGGAA AACCGCTCCT GCCAAAAGAA 
GCCGAGGAAC AACTTCCACC CTTAAGCGAT GACTTCCTTC TTGATGCATT GTCTCAGGAC 
TTCTCCAGTC CTGCAAACAT ATTGTCTCTT GGTTTTGAAG ATGCCAAACT TTCTGCTGCT 
GTTTCTGAAA CAGTTTCCCA GGTGCCTGCT CCAAGCAACC ACACAGCAGC CCCACCGCCT 
GGCACTGAGA GGAGAGACAA AGAACTCGAT GATGCCTTGG ATGAACTTTC TGACAGTCTT 
GGACAAAGAC AGCCTGATCC AGATGAGAAC AAACCACTAG ATGATAAAGT AAAGGAAAAA 
ATCAAAGCAG AGCATAGCGA GAAACTGGGA GAAAGAGACG ACACCATCCC CCCTGAATAT 
AGGCATCTCC TGGATAATGA TGGGAAGGAC AAACCAGAGA AGCCACTGAC AAAGAACACA 
GAGAAGCCTG GTCAGGACCA GGACCCCATT GATGCCCTCT CAGAAGACTT GGATAGCTGC 
CCCCCAACTA CAGAGACTTC ACAGAATACA ACAAAGGAGA AGGGTAAGAA GACTTCAAGT 
TCCAAAGCAT CCAAGAATGA AGAGAAAACA AAGGATTCCT CCAAGAAGAC AGAGGAAGTG 
CCCAAGCCAA AGGTGGATGA AGATGCAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.
Cell reports16(11)2901-2913(2016 09)
Mochida S,Hida Y,Tanifuji S,Hagiwara A,Hamada S,Abe M,Ma H,Yasumura M,Kitajima I,Sakimura K,Ohtsuka T

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages.
The Journal of biological chemistry286(40)34830-8(2011 Oct)
Huang Z,Hoffmann FW,Norton RL,Hashimoto AC,Hoffmann PR

Calpain activation promotes BACE1 expression, amyloid precursor protein processing, and amyloid plaque formation in a transgenic mouse model of Alzheimer disease.
The Journal of biological chemistry285(36)27737-44(2010 Sep)
Liang B,Duan BY,Zhou XP,Gong JX,Luo ZG

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Cast cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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