Cast gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_011244467.2	XP_011242769.1	calpastatin isoform X4
NM_001301153.1	NP_001288082.1	calpastatin isoform 1
NM_001301158.1	NP_001288087.1	calpastatin isoform 6
XM_006517059.1	XP_006517122.1	calpastatin isoform X3
XM_011244467.3	XP_011242769.1	calpastatin isoform X4
XM_011244469.3	XP_011242771.1	calpastatin isoform X6
NM_001301181.1	NP_001288110.1	calpastatin isoform 8
NM_001301155.1	NP_001288084.1	calpastatin isoform 3
NM_009817.4	NP_033947.1	calpastatin isoform 2
XM_011244466.2	XP_011242768.1	calpastatin isoform X2
XM_011244469.2	XP_011242771.1	calpastatin isoform X6
XM_017315374.1	XP_017170863.1	calpastatin isoform X5
NM_001301160.1	NP_001288089.1	calpastatin isoform 7
NM_001301156.1	NP_001288085.1	calpastatin isoform 4
NM_001301157.1	NP_001288086.1	calpastatin isoform 5
XM_006517056.2	XP_006517119.1	calpastatin isoform X1
XM_011244466.3	XP_011242768.1	calpastatin isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67314	NM_001301157.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu67315	NM_001301160.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu67316	NM_001301158.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu67317	NM_001301181.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu67312	XM_011244469.3 Latest version!	Mus musculus calpastatin (Cast), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu15612	NM_009817.4 Latest version!	Mus musculus calpastatin (Cast), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$447.30 $639.00
OMu67313	NM_001301155.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu67318	NM_001301156.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu67319	NM_001301153.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu48635	XM_006517056.2 Latest version!	Mus musculus calpastatin (Cast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu48638	XM_006517059.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu67309	XM_011244466.3 Latest version!	Mus musculus calpastatin (Cast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu67310	XM_011244467.3 Latest version!	Mus musculus calpastatin (Cast), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu78421	XM_017315374.1 Latest version!	Mus musculus calpastatin (Cast), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu67310	XM_011244467.2	Mus musculus calpastatin (Cast), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu67312	XM_011244469.2	Mus musculus calpastatin (Cast), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu67309	XM_011244466.2	Mus musculus calpastatin (Cast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

Cast ( NM_001301157.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288086.1 Mus musculus calpastatin (Cast), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu67314
Clone ID Related Accession (Same CDS sequence)	NM_001301157.1
Accession Version	NM_001301157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	calpastatin isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ072297.1, BC010603.1, AK083395.1 and AK037098.1. Transcript Variant: This variant (5) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (5) with a shorter N-terminus. This variant also lacks two internal exons relative to variant 1. The resulting protein (isoform 5) has a shorter N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTACCA CAGAGACTAA GGCTGTGAAA ACAGAGTCTA AGAAACCACA ATCAAGTGAG 
CAACCTGTGG TTCACGAGAA AAAGTCAAAA GGAGGGCCAA AGGAAGGGTC AGAGCCCAAA 
AATCTTCCGA AGCACACATC AAGTACAGGA AGCAAGCATG CTCATAAAGA AAAAGCACTT 
TCCAGATCAA ATGAACAGAT GGTGTCAGAG AAACCATCAG AATCAAAGAC AAAATTTCAA 
GATGTTCCTT CAGCTGGTGG AGAGAGTGTG GCTGGAGGTG GGACTGTGGC CACTGCCTTG 
GACAAAGTGG TTGGTAAGAA AAAAGAACAG AAACCATTCA CACCAGCTTC ACCTGTGCAG 
TCCACACCGA GCAAGCCATC TGACAAGTCA GGCATGGATG CTGCCTTGGA TGACCTGATA 
GACACCTTAG GTGGACATGA AGATACTAAC AGAGATGATC CACCATATAC TGGACCAGTA 
GTTTTGGACC CAATGTATTC TACCTACCTT GAGGCACTGG GTATAAAAGA AGGGACTATT 
CCTCCAGAGT ATAGGAAACT TTTGGAAAAA AATGAAGGGA TCACACAACC TCTTCCAGAC 
TCTCCTAAAC CTATGGGGAC TGACCAGGCT ATAGATGCCT TGTCATCTGA TTTCACCTGT 
AGTTCTCCAA CTGGAAAGCA AAGTGAGAAA GAGAAGTCTA CTGGGGAAAT TTTCAAAGCT 
CAGTCTGCAG GAGTGACCAG AAGTTCTGTT CCCCCCAAGG AGAAGAAAAG GAAGGTGGAA 
GAGGAGGTCA TAAATGATCA AGCACTTCAG GCTCTGTCAG ATTCACTGGG CACCCGGCAG 
CCAGATCCTC CGAGCCACGT TAGCCAAGCT GAACAAGTCA AAGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GCAGAAAGAC 
AAAGAACTTG ATGATGCCTT GGATGAACTT TCTGACAGTC TTGGACAAAG GCCGCCTGAT 
CCAGATGAGA ACAAACCACT GGATGACAAA GTGAAGGAGA AAATCAAACC AGAGCATAGC 
GAGAAACTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ACAGGCATCT CTTGGATAAT 
GATGGGAAGA AACCTGATCA GGACCGGGAC CCCATTGATG CCCTCTCAGA AGATTTGGAT 
AGCTGCCCCT CAACTACAGA GACCTCAAAG AATACAGCAA AGGGGAAGAG CAAGAAGACT 
TCAAGTTCCA AAGCATCCAA GGACGGAGAG AAAACAAAGG ACTCTTCCAA GAAGACAGAG 
GAAGTGTCCA AGCCAAAGGC TAAAGAAGAT GCAAGACACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288086.1
CDS	231..2255
Translation	MSTTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	NM_001301157.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301157.1

ORF Insert Sequence:

ATGAGTACCA CAGAGACTAA GGCTGTGAAA ACAGAGTCTA AGAAACCACA ATCAAGTGAG 
CAACCTGTGG TTCACGAGAA AAAGTCAAAA GGAGGGCCAA AGGAAGGGTC AGAGCCCAAA 
AATCTTCCGA AGCACACATC AAGTACAGGA AGCAAGCATG CTCATAAAGA AAAAGCACTT 
TCCAGATCAA ATGAACAGAT GGTGTCAGAG AAACCATCAG AATCAAAGAC AAAATTTCAA 
GATGTTCCTT CAGCTGGTGG AGAGAGTGTG GCTGGAGGTG GGACTGTGGC CACTGCCTTG 
GACAAAGTGG TTGGTAAGAA AAAAGAACAG AAACCATTCA CACCAGCTTC ACCTGTGCAG 
TCCACACCGA GCAAGCCATC TGACAAGTCA GGCATGGATG CTGCCTTGGA TGACCTGATA 
GACACCTTAG GTGGACATGA AGATACTAAC AGAGATGATC CACCATATAC TGGACCAGTA 
GTTTTGGACC CAATGTATTC TACCTACCTT GAGGCACTGG GTATAAAAGA AGGGACTATT 
CCTCCAGAGT ATAGGAAACT TTTGGAAAAA AATGAAGGGA TCACACAACC TCTTCCAGAC 
TCTCCTAAAC CTATGGGGAC TGACCAGGCT ATAGATGCCT TGTCATCTGA TTTCACCTGT 
AGTTCTCCAA CTGGAAAGCA AAGTGAGAAA GAGAAGTCTA CTGGGGAAAT TTTCAAAGCT 
CAGTCTGCAG GAGTGACCAG AAGTTCTGTT CCCCCCAAGG AGAAGAAAAG GAAGGTGGAA 
GAGGAGGTCA TAAATGATCA AGCACTTCAG GCTCTGTCAG ATTCACTGGG CACCCGGCAG 
CCAGATCCTC CGAGCCACGT TAGCCAAGCT GAACAAGTCA AAGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GCAGAAAGAC 
AAAGAACTTG ATGATGCCTT GGATGAACTT TCTGACAGTC TTGGACAAAG GCCGCCTGAT 
CCAGATGAGA ACAAACCACT GGATGACAAA GTGAAGGAGA AAATCAAACC AGAGCATAGC 
GAGAAACTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ACAGGCATCT CTTGGATAAT 
GATGGGAAGA AACCTGATCA GGACCGGGAC CCCATTGATG CCCTCTCAGA AGATTTGGAT 
AGCTGCCCCT CAACTACAGA GACCTCAAAG AATACAGCAA AGGGGAAGAG CAAGAAGACT 
TCAAGTTCCA AAGCATCCAA GGACGGAGAG AAAACAAAGG ACTCTTCCAA GAAGACAGAG 
GAAGTGTCCA AGCCAAAGGC TAAAGAAGAT GCAAGACACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_001301160.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288089.1 Mus musculus calpastatin (Cast), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OMu67315
Clone ID Related Accession (Same CDS sequence)	NM_001301160.1
Accession Version	NM_001301160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	calpastatin isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK006253.1, AK161284.1, AK029293.1, AK083395.1 and AK037098.1. On Jul 26, 2014 this sequence version replaced XM_006517070.1. Transcript Variant: This variant (7) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate start codon and result in an isoform (7) with a shorter N-terminus. The resulting protein (isoform 7) has a shorter and distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK161284.1, AF190151.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GCAAAAGCAA AAGAAGAAAG GCAGGAGAAG TGTGGTGAAG ATGAGGACAC AGTCCCAGCT 
GAGTACAGGT TAAAACCAGC AAAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAACATCTA AGAGCCTGAG TGAGTCGGAG CTGATTGGGG AGCTTTCAGC AGATTTTGAC 
CGATCTACAT ATCAAGACAA ACCATCTACG CCAGCTGAAA AGAAATCCAA TGACACATCC 
CAAACTCCTC CGGGGGAGAC TGTGCCTCGG GCCTCCATGT GCAGTATACG GTCAGCGCCA 
CCCAAACTAG CATCCTTGAA GGGCGTGGTA CCAGAAGATG CTGTTGAAAC CTTGGCTGGA 
AGCCTGGGGA CAAGGGAAGC AGATCCAGAA CATGAAAAAA CTGTGGAGGA TAAAGTCAAG 
GAGAAAGCTA AAGAAGAAGA GCATGAAAAA CTTGGTGAAA AAGAAGAAAC AGTGCCTCCT 
GATTATCGAC TAGAAGAAGT CAAGGATAAG GATGGAAAAC CACTCCTGCC CAAAGAATCC 
CAGGAACAAC TTGCACCCTT AAGCGATGAC TTCCTTCTTG ATGCCTTGTC TCAGGACTTC 
TCCAGTCCGG CAAACATATC GTCTCTTGAA TTTGAAGATG CCAAACTTTC TGCTGCCATT 
TCTGAAGTAG TTTCTCAGAC ACCTGCTCCA AGCACCCATG CAGCAGCTCC ACTGCCTGGC 
ACTGAGCAGA AAGACAAAGA ACTTGATGAT GCCTTGGATG AACTTTCTGA CAGTCTTGGA 
CAAAGGCCGC CTGATCCAGA TGAGAACAAA CCACTGGATG ACAAAGTGAA GGAGAAAATC 
AAACCAGAGC ATAGCGAGAA ACTGGGAGAA AGAGACGACA CCATCCCCCC TGAATACAGG 
CATCTCTTGG ATAATGATGG GAAGGACAAA CCAGAGAAGC CACCGACTAA GAAAACAGAG 
AAACCTGATC AGGACCGGGA CCCCATTGAT GCCCTCTCAG AAGATTTGGA TAGCTGCCCC 
TCAACTACAG AGACCTCAAA GAATACAGCA AAGGGGAAGA GCAAGAAGAC TTCAAGTTCC 
AAAGCATCCA AGGACGGAGA GAAAACAAAG GACTCTTCCA AGAAGACAGA GGAAGTGTCC 
AAGCCAAAGG CTAAAGAAGA TGCAAGACAC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288089.1
CDS	85..1380
Translation	MGQFLSSTFWEGSPAAVWQEKLREGERKGAGETIPILQDHAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	NM_001301160.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301160.1

ORF Insert Sequence:

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GCAAAAGCAA AAGAAGAAAG GCAGGAGAAG TGTGGTGAAG ATGAGGACAC AGTCCCAGCT 
GAGTACAGGT TAAAACCAGC AAAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAACATCTA AGAGCCTGAG TGAGTCGGAG CTGATTGGGG AGCTTTCAGC AGATTTTGAC 
CGATCTACAT ATCAAGACAA ACCATCTACG CCAGCTGAAA AGAAATCCAA TGACACATCC 
CAAACTCCTC CGGGGGAGAC TGTGCCTCGG GCCTCCATGT GCAGTATACG GTCAGCGCCA 
CCCAAACTAG CATCCTTGAA GGGCGTGGTA CCAGAAGATG CTGTTGAAAC CTTGGCTGGA 
AGCCTGGGGA CAAGGGAAGC AGATCCAGAA CATGAAAAAA CTGTGGAGGA TAAAGTCAAG 
GAGAAAGCTA AAGAAGAAGA GCATGAAAAA CTTGGTGAAA AAGAAGAAAC AGTGCCTCCT 
GATTATCGAC TAGAAGAAGT CAAGGATAAG GATGGAAAAC CACTCCTGCC CAAAGAATCC 
CAGGAACAAC TTGCACCCTT AAGCGATGAC TTCCTTCTTG ATGCCTTGTC TCAGGACTTC 
TCCAGTCCGG CAAACATATC GTCTCTTGAA TTTGAAGATG CCAAACTTTC TGCTGCCATT 
TCTGAAGTAG TTTCTCAGAC ACCTGCTCCA AGCACCCATG CAGCAGCTCC ACTGCCTGGC 
ACTGAGCAGA AAGACAAAGA ACTTGATGAT GCCTTGGATG AACTTTCTGA CAGTCTTGGA 
CAAAGGCCGC CTGATCCAGA TGAGAACAAA CCACTGGATG ACAAAGTGAA GGAGAAAATC 
AAACCAGAGC ATAGCGAGAA ACTGGGAGAA AGAGACGACA CCATCCCCCC TGAATACAGG 
CATCTCTTGG ATAATGATGG GAAGGACAAA CCAGAGAAGC CACCGACTAA GAAAACAGAG 
AAACCTGATC AGGACCGGGA CCCCATTGAT GCCCTCTCAG AAGATTTGGA TAGCTGCCCC 
TCAACTACAG AGACCTCAAA GAATACAGCA AAGGGGAAGA GCAAGAAGAC TTCAAGTTCC 
AAAGCATCCA AGGACGGAGA GAAAACAAAG GACTCTTCCA AGAAGACAGA GGAAGTGTCC 
AAGCCAAAGG CTAAAGAAGA TGCAAGACAC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_001301158.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288087.1 Mus musculus calpastatin (Cast), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OMu67316
Clone ID Related Accession (Same CDS sequence)	NM_001301158.1
Accession Version	NM_001301158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	calpastatin isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK006253.1, BY086784.1, AK029293.1, AK083395.1 and AK037098.1. On Jul 26, 2014 this sequence version replaced XM_006517068.1. Transcript Variant: This variant (6) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate start codon and result in an isoform (6) with a shorter and distinct N-terminus. This variant (6) also uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting protein (isoform 6) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK006253.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GTAATATGCT CAGAAGAAAG GGAACATGGG TCCAAACACC ATGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GAAAGACAAA 
GAACTTGATG ATGCCTTGGA TGAACTTTCT GACAGTCTTG GACAAAGGCC GCCTGATCCA 
GATGAGAACA AACCACTGGA TGACAAAGTG AAGGAGAAAA TCAAACCAGA GCATAGCGAG 
AAACTGGGAG AAAGAGACGA CACCATCCCC CCTGAATACA GGCATCTCTT GGATAATGAT 
GGGAAGGACA AACCAGAGAA GCCACCGACT AAGAAAACAG AGAAACCTGA TCAGGACCGG 
GACCCCATTG ATGCCCTCTC AGAAGATTTG GATAGCTGCC CCTCAACTAC AGAGACCTCA 
AAGAATACAG CAAAGGGGAA GAGCAAGAAG ACTTCAAGTT CCAAAGCATC CAAGGACGGA 
GAGAAAACAA AGGACTCTTC CAAGAAGACA GAGGAAGTGT CCAAGCCAAA GGCTAAAGAA 
GATGCAAGAC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288087.1
CDS	85..1422
Translation	MGQFLSSTFWEGSPAAVWQEKLREGERKGAGETIPILQDHVICSEEREHGSKHHEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	NM_001301158.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301158.1

ORF Insert Sequence:

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GTAATATGCT CAGAAGAAAG GGAACATGGG TCCAAACACC ATGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GAAAGACAAA 
GAACTTGATG ATGCCTTGGA TGAACTTTCT GACAGTCTTG GACAAAGGCC GCCTGATCCA 
GATGAGAACA AACCACTGGA TGACAAAGTG AAGGAGAAAA TCAAACCAGA GCATAGCGAG 
AAACTGGGAG AAAGAGACGA CACCATCCCC CCTGAATACA GGCATCTCTT GGATAATGAT 
GGGAAGGACA AACCAGAGAA GCCACCGACT AAGAAAACAG AGAAACCTGA TCAGGACCGG 
GACCCCATTG ATGCCCTCTC AGAAGATTTG GATAGCTGCC CCTCAACTAC AGAGACCTCA 
AAGAATACAG CAAAGGGGAA GAGCAAGAAG ACTTCAAGTT CCAAAGCATC CAAGGACGGA 
GAGAAAACAA AGGACTCTTC CAAGAAGACA GAGGAAGTGT CCAAGCCAAA GGCTAAAGAA 
GATGCAAGAC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_001301181.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288110.1 Mus musculus calpastatin (Cast), transcript variant 8, mRNA.

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CloneID	OMu67317
Clone ID Related Accession (Same CDS sequence)	NM_001301181.1
Accession Version	NM_001301181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	calpastatin isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK006253.1, BY086784.1, AK161284.1, AK083395.1 and AK037098.1. On Jul 26, 2014 this sequence version replaced XM_006517067.1. Transcript Variant: This variant (8) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate start codon and result in an isoform (8) with a shorter and distinct N-terminus. The resulting protein (isoform 8) has a distinct and shorter N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF190152.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GTAATATGCT CAGAAGAAAG GGAACATGGG TCCAAACACC ATGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GCAGAAAGAC 
AAAGAACTTG ATGATGCCTT GGATGAACTT TCTGACAGTC TTGGACAAAG GCCGCCTGAT 
CCAGATGAGA ACAAACCACT GGATGACAAA GTGAAGGAGA AAATCAAACC AGAGCATAGC 
GAGAAACTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ACAGGCATCT CTTGGATAAT 
GATGGGAAGG ACAAACCAGA GAAGCCACCG ACTAAGAAAA CAGAGAAACC TGATCAGGAC 
CGGGACCCCA TTGATGCCCT CTCAGAAGAT TTGGATAGCT GCCCCTCAAC TACAGAGACC 
TCAAAGAATA CAGCAAAGGG GAAGAGCAAG AAGACTTCAA GTTCCAAAGC ATCCAAGGAC 
GGAGAGAAAA CAAAGGACTC TTCCAAGAAG ACAGAGGAAG TGTCCAAGCC AAAGGCTAAA 
GAAGATGCAA GACACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288110.1
CDS	85..1425
Translation	MGQFLSSTFWEGSPAAVWQEKLREGERKGAGETIPILQDHVICSEEREHGSKHHEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	NM_001301181.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301181.1

ORF Insert Sequence:

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GTAATATGCT CAGAAGAAAG GGAACATGGG TCCAAACACC ATGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GCAGAAAGAC 
AAAGAACTTG ATGATGCCTT GGATGAACTT TCTGACAGTC TTGGACAAAG GCCGCCTGAT 
CCAGATGAGA ACAAACCACT GGATGACAAA GTGAAGGAGA AAATCAAACC AGAGCATAGC 
GAGAAACTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ACAGGCATCT CTTGGATAAT 
GATGGGAAGG ACAAACCAGA GAAGCCACCG ACTAAGAAAA CAGAGAAACC TGATCAGGAC 
CGGGACCCCA TTGATGCCCT CTCAGAAGAT TTGGATAGCT GCCCCTCAAC TACAGAGACC 
TCAAAGAATA CAGCAAAGGG GAAGAGCAAG AAGACTTCAA GTTCCAAAGC ATCCAAGGAC 
GGAGAGAAAA CAAAGGACTC TTCCAAGAAG ACAGAGGAAG TGTCCAAGCC AAAGGCTAAA 
GAAGATGCAA GACACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_011244469.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242771.1 Mus musculus calpastatin (Cast), transcript variant X6, mRNA.

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CloneID	OMu67312
Clone ID Related Accession (Same CDS sequence)	XM_011244469.3 , XM_011244469.2
Accession Version	XM_011244469.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GCAAAAGCAA AAGAAGAAAG GCAGGAGAAG TGTGGTGAAG ATGAGGACAC AGTCCCAGCT 
GAGTACAGGT TAAAACCAGC AAAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAACATCTA AGAGCCTGAG TGAGTCGGAG CTGATTGGGG AGCTTTCAGC AGATTTTGAC 
CGATCTACAT ATCAAGACAA ACCATCTACG CCAGCTGAAA AGAAATCCAA TGACACATCC 
CAAACTCCTC CGGGGGAGAC TGTGCCTCGG GCCTCCATGT GCAGTATACG GTCAGCGCCA 
CCCAAACTAG CATCCTTGAA GGGCGTGGTA CCAGAAGATG CTGTTGAAAC CTTGGCTGGA 
AGCCTGGGGA CAAGGGAAGC AGATCCAGAA CATGAAAAAA CTGTGGAGGA TAAAGTCAAG 
GAGAAAGCTA AAGAAGAAGA GCATGAAAAA CTTGGTGAAA AAGAAGAAAC AGTGCCTCCT 
GATTATCGAC TAGAAGAAGT CAAGGATAAG GATGGAAAAC CACTCCTGCC CAAAGAATCC 
CAGGAACAAC TTGCACCCTT AAGCGATGAC TTCCTTCTTG ATGCCTTGTC TCAGGACTTC 
TCCAGTCCGG CAAACATATC GTCTCTTGAA TTTGAAGATG CCAAACTTTC TGCTGCCATT 
TCTGAAGTAG TTTCTCAGAC ACCTGCTCCA AGCACCCATG CAGCAGCTCC ACTGCCTGGC 
ACTGAGAAAG ACAAAGAACT TGATGATGCC TTGGATGAAC TTTCTGACAG TCTTGGACAA 
AGGCCGCCTG ATCCAGATGA GAACAAACCA CTGGATGACA AAGTGAAGGA GAAAATCAAA 
CCAGAGCATA GCGAGAAACT GGGAGAAAGA GACGACACCA TCCCCCCTGA ATACAGGCAT 
CTCTTGGATA ATGATGGGAA GGACAAACCA GAGAAGCCAC CGACTAAGAA AACAGAGAAA 
CCTGATCAGG ACCGGGACCC CATTGATGCC CTCTCAGAAG ATTTGGATAG CTGCCCCTCA 
ACTACAGAGA CCTCAAAGAA TACAGCAAAG GGGAAGAGCA AGAAGACTTC AAGTTCCAAA 
GCATCCAAGG ACGGAGAGAA AACAAAGGAC TCTTCCAAGA AGACAGAGGA AGTGTCCAAG 
CCAAAGGCTA AAGAAGATGC AAGACACAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242771.1
CDS	81..1373
Translation	MGQFLSSTFWEGSPAAVWQEKLREGERKGAGETIPILQDHAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_011244469.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244469.2

ORF Insert Sequence:

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GCAAAAGCAA AAGAAGAAAG GCAGGAGAAG TGTGGTGAAG ATGAGGACAC AGTCCCAGCT 
GAGTACAGGT TAAAACCAGC AAAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAACATCTA AGAGCCTGAG TGAGTCGGAG CTGATTGGGG AGCTTTCAGC AGATTTTGAC 
CGATCTACAT ATCAAGACAA ACCATCTACG CCAGCTGAAA AGAAATCCAA TGACACATCC 
CAAACTCCTC CGGGGGAGAC TGTGCCTCGG GCCTCCATGT GCAGTATACG GTCAGCGCCA 
CCCAAACTAG CATCCTTGAA GGGCGTGGTA CCAGAAGATG CTGTTGAAAC CTTGGCTGGA 
AGCCTGGGGA CAAGGGAAGC AGATCCAGAA CATGAAAAAA CTGTGGAGGA TAAAGTCAAG 
GAGAAAGCTA AAGAAGAAGA GCATGAAAAA CTTGGTGAAA AAGAAGAAAC AGTGCCTCCT 
GATTATCGAC TAGAAGAAGT CAAGGATAAG GATGGAAAAC CACTCCTGCC CAAAGAATCC 
CAGGAACAAC TTGCACCCTT AAGCGATGAC TTCCTTCTTG ATGCCTTGTC TCAGGACTTC 
TCCAGTCCGG CAAACATATC GTCTCTTGAA TTTGAAGATG CCAAACTTTC TGCTGCCATT 
TCTGAAGTAG TTTCTCAGAC ACCTGCTCCA AGCACCCATG CAGCAGCTCC ACTGCCTGGC 
ACTGAGAAAG ACAAAGAACT TGATGATGCC TTGGATGAAC TTTCTGACAG TCTTGGACAA 
AGGCCGCCTG ATCCAGATGA GAACAAACCA CTGGATGACA AAGTGAAGGA GAAAATCAAA 
CCAGAGCATA GCGAGAAACT GGGAGAAAGA GACGACACCA TCCCCCCTGA ATACAGGCAT 
CTCTTGGATA ATGATGGGAA GGACAAACCA GAGAAGCCAC CGACTAAGAA AACAGAGAAA 
CCTGATCAGG ACCGGGACCC CATTGATGCC CTCTCAGAAG ATTTGGATAG CTGCCCCTCA 
ACTACAGAGA CCTCAAAGAA TACAGCAAAG GGGAAGAGCA AGAAGACTTC AAGTTCCAAA 
GCATCCAAGG ACGGAGAGAA AACAAAGGAC TCTTCCAAGA AGACAGAGGA AGTGTCCAAG 
CCAAAGGCTA AAGAAGATGC AAGACACAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_011244469.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242771.1 Mus musculus calpastatin (Cast), transcript variant X6, mRNA.

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CloneID	OMu67312
Clone ID Related Accession (Same CDS sequence)	XM_011244469.3 , XM_011244469.2
Accession Version	XM_011244469.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244469.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GCAAAAGCAA AAGAAGAAAG GCAGGAGAAG TGTGGTGAAG ATGAGGACAC AGTCCCAGCT 
GAGTACAGGT TAAAACCAGC AAAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAACATCTA AGAGCCTGAG TGAGTCGGAG CTGATTGGGG AGCTTTCAGC AGATTTTGAC 
CGATCTACAT ATCAAGACAA ACCATCTACG CCAGCTGAAA AGAAATCCAA TGACACATCC 
CAAACTCCTC CGGGGGAGAC TGTGCCTCGG GCCTCCATGT GCAGTATACG GTCAGCGCCA 
CCCAAACTAG CATCCTTGAA GGGCGTGGTA CCAGAAGATG CTGTTGAAAC CTTGGCTGGA 
AGCCTGGGGA CAAGGGAAGC AGATCCAGAA CATGAAAAAA CTGTGGAGGA TAAAGTCAAG 
GAGAAAGCTA AAGAAGAAGA GCATGAAAAA CTTGGTGAAA AAGAAGAAAC AGTGCCTCCT 
GATTATCGAC TAGAAGAAGT CAAGGATAAG GATGGAAAAC CACTCCTGCC CAAAGAATCC 
CAGGAACAAC TTGCACCCTT AAGCGATGAC TTCCTTCTTG ATGCCTTGTC TCAGGACTTC 
TCCAGTCCGG CAAACATATC GTCTCTTGAA TTTGAAGATG CCAAACTTTC TGCTGCCATT 
TCTGAAGTAG TTTCTCAGAC ACCTGCTCCA AGCACCCATG CAGCAGCTCC ACTGCCTGGC 
ACTGAGAAAG ACAAAGAACT TGATGATGCC TTGGATGAAC TTTCTGACAG TCTTGGACAA 
AGGCCGCCTG ATCCAGATGA GAACAAACCA CTGGATGACA AAGTGAAGGA GAAAATCAAA 
CCAGAGCATA GCGAGAAACT GGGAGAAAGA GACGACACCA TCCCCCCTGA ATACAGGCAT 
CTCTTGGATA ATGATGGGAA GGACAAACCA GAGAAGCCAC CGACTAAGAA AACAGAGAAA 
CCTGATCAGG ACCGGGACCC CATTGATGCC CTCTCAGAAG ATTTGGATAG CTGCCCCTCA 
ACTACAGAGA CCTCAAAGAA TACAGCAAAG GGGAAGAGCA AGAAGACTTC AAGTTCCAAA 
GCATCCAAGG ACGGAGAGAA AACAAAGGAC TCTTCCAAGA AGACAGAGGA AGTGTCCAAG 
CCAAAGGCTA AAGAAGATGC AAGACACAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242771.1
CDS	80..1372
Translation	MGQFLSSTFWEGSPAAVWQEKLREGERKGAGETIPILQDHAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_011244469.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244469.3

ORF Insert Sequence:

ATGGGCCAGT TTCTGTCTTC TACTTTCTGG GAGGGCTCAC CTGCTGCAGT GTGGCAAGAA 
AAGCTTCGTG AGGGTGAACG AAAGGGAGCT GGAGAAACCA TCCCTATCCT CCAGGACCAT 
GCAAAAGCAA AAGAAGAAAG GCAGGAGAAG TGTGGTGAAG ATGAGGACAC AGTCCCAGCT 
GAGTACAGGT TAAAACCAGC AAAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAACATCTA AGAGCCTGAG TGAGTCGGAG CTGATTGGGG AGCTTTCAGC AGATTTTGAC 
CGATCTACAT ATCAAGACAA ACCATCTACG CCAGCTGAAA AGAAATCCAA TGACACATCC 
CAAACTCCTC CGGGGGAGAC TGTGCCTCGG GCCTCCATGT GCAGTATACG GTCAGCGCCA 
CCCAAACTAG CATCCTTGAA GGGCGTGGTA CCAGAAGATG CTGTTGAAAC CTTGGCTGGA 
AGCCTGGGGA CAAGGGAAGC AGATCCAGAA CATGAAAAAA CTGTGGAGGA TAAAGTCAAG 
GAGAAAGCTA AAGAAGAAGA GCATGAAAAA CTTGGTGAAA AAGAAGAAAC AGTGCCTCCT 
GATTATCGAC TAGAAGAAGT CAAGGATAAG GATGGAAAAC CACTCCTGCC CAAAGAATCC 
CAGGAACAAC TTGCACCCTT AAGCGATGAC TTCCTTCTTG ATGCCTTGTC TCAGGACTTC 
TCCAGTCCGG CAAACATATC GTCTCTTGAA TTTGAAGATG CCAAACTTTC TGCTGCCATT 
TCTGAAGTAG TTTCTCAGAC ACCTGCTCCA AGCACCCATG CAGCAGCTCC ACTGCCTGGC 
ACTGAGAAAG ACAAAGAACT TGATGATGCC TTGGATGAAC TTTCTGACAG TCTTGGACAA 
AGGCCGCCTG ATCCAGATGA GAACAAACCA CTGGATGACA AAGTGAAGGA GAAAATCAAA 
CCAGAGCATA GCGAGAAACT GGGAGAAAGA GACGACACCA TCCCCCCTGA ATACAGGCAT 
CTCTTGGATA ATGATGGGAA GGACAAACCA GAGAAGCCAC CGACTAAGAA AACAGAGAAA 
CCTGATCAGG ACCGGGACCC CATTGATGCC CTCTCAGAAG ATTTGGATAG CTGCCCCTCA 
ACTACAGAGA CCTCAAAGAA TACAGCAAAG GGGAAGAGCA AGAAGACTTC AAGTTCCAAA 
GCATCCAAGG ACGGAGAGAA AACAAAGGAC TCTTCCAAGA AGACAGAGGA AGTGTCCAAG 
CCAAAGGCTA AAGAAGATGC AAGACACAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_009817.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033947.1 Mus musculus calpastatin (Cast), transcript variant 2, mRNA.

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CloneID	OMu15612
Clone ID Related Accession (Same CDS sequence)	NM_009817.4
Accession Version	NM_009817.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	calpastatin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD550676.1, AK028843.1, AK083395.1 and AK037098.1. On Jul 26, 2014 this sequence version replaced NM_009817.3. Transcript Variant: This variant (2) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate start codon and result in an isoform (2) with a shorter and distinct N-terminus. This variant also lacks one internal exons relative to variant 1. The resulting protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028843.1, AK028841.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATTCG CAAGTTGGTG GTACAAGACG CATGTTAATG AAAAGAATAT TGGATCATCT 
TCTAAACCAG GAGAAAAGAA AGGATCCGAT GAGAAAAAAG CAGCAAGTTT GGGGAGCAGT 
CAGCCTTCCA GACCACATGT TGGTGAAGCT GCCACAGCCA CCAAGGTGAC AGCTTCCTCT 
GCTGCCACCA GCAAGTCCCC CAGCATGAGT ACCACAGAGA CTAAGGCTGT GAAAACAGAG 
TCTAAGAAAC CACAATCAAG TGAGCAACCT GTGGTTCACG AGAAAAAGTC AAAAGGAGGG 
CCAAAGGAAG GGTCAGAGCC CAAAAATCTT CCGAAGCACA CATCAAGTAC AGGAAGCAAG 
CATGCTCATA AAGAAAAAGC ACTTTCCAGA TCAAATGAAC AGATGGTGTC AGAGAAACCA 
TCAGAATCAA AGACAAAATT TCAAGATGTT CCTTCAGCTG GTGGAGAGAG TGTGGCTGGA 
GGTGGGACTG TGGCCACTGC CTTGGACAAA GTGGTTGGTA AGAAAAAAGA ACAGAAACCA 
TTCACACCAG CTTCACCTGT GCAGTCCACA CCGAGCAAGC CATCTGACAA GTCAGGCATG 
GATGCTGCCT TGGATGACCT GATAGACACC TTAGGTGGAC ATGAAGATAC TAACAGAGAT 
GATCCACCAT ATACTGGACC AGTAGTTTTG GACCCAATGT ATTCTACCTA CCTTGAGGCA 
CTGGGTATAA AAGAAGGGAC TATTCCTCCA GAGTATAGGA AACTTTTGGA AAAAAATGAA 
GGGATCACAC AACCTCTTCC AGACTCTCCT AAACCTATGG GGACTGACCA GGCTATAGAT 
GCCTTGTCAT CTGATTTCAC CTGTAGTTCT CCAACTGGAA AGCAAAGTGA GAAAGAGAAG 
TCTACTGGGG AAATTTTCAA AGCTCAGTCT GCAGGAGTGA CCAGAAGTTC TGTTCCCCCC 
AAGGAGAAGA AAAGGAAGGT GGAAGAGGAG GTCATAAATG ATCAAGCACT TCAGGCTCTG 
TCAGATTCAC TGGGCACCCG GCAGCCAGAT CCTCCGAGCC ACGTTAGCCA AGCTGAACAA 
GTCAAAGAGG CAAAAGCAAA AGAAGAAAGG CAGGAGAAGT GTGGTGAAGA TGAGGACACA 
GTCCCAGCTG AGTACAGGTT AAAACCAGCA AAGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAACATCTAA GAGCCTGAGT GAGTCGGAGC TGATTGGGGA GCTTTCAGCA 
GATTTTGACC GATCTACATA TCAAGACAAA CCATCTACGC CAGCTGAAAA GAAATCCAAT 
GACACATCCC AAACTCCTCC GGGGGAGACT GTGCCTCGGG CCTCCATGTG CAGTATACGG 
TCAGCGCCAC CCAAACTAGC ATCCTTGAAG GGCGTGGTAC CAGAAGATGC TGTTGAAACC 
TTGGCTGGAA GCCTGGGGAC AAGGGAAGCA GATCCAGAAC ATGAAAAAAC TGTGGAGGAT 
AAAGTCAAGG AGAAAGCTAA AGAAGAAGAG CATGAAAAAC TTGGTGAAAA AGAAGAAACA 
GTGCCTCCTG ATTATCGACT AGAAGAAGTC AAGGATAAGG ATGGAAAACC ACTCCTGCCC 
AAAGAATCCC AGGAACAACT TGCACCCTTA AGCGATGACT TCCTTCTTGA TGCCTTGTCT 
CAGGACTTCT CCAGTCCGGC AAACATATCG TCTCTTGAAT TTGAAGATGC CAAACTTTCT 
GCTGCCATTT CTGAAGTAGT TTCTCAGACA CCTGCTCCAA GCACCCATGC AGCAGCTCCA 
CTGCCTGGCA CTGAGCAGAA AGACAAAGAA CTTGATGATG CCTTGGATGA ACTTTCTGAC 
AGTCTTGGAC AAAGGCCGCC TGATCCAGAT GAGAACAAAC CACTGGATGA CAAAGTGAAG 
GAGAAAATCA AACCAGAGCA TAGCGAGAAA CTGGGAGAAA GAGACGACAC CATCCCCCCT 
GAATACAGGC ATCTCTTGGA TAATGATGGG AAGGACAAAC CAGAGAAGCC ACCGACTAAG 
AAAACAGAGA AACCTGATCA GGACCGGGAC CCCATTGATG CCCTCTCAGA AGATTTGGAT 
AGCTGCCCCT CAACTACAGA GACCTCAAAG AATACAGCAA AGGGGAAGAG CAAGAAGACT 
TCAAGTTCCA AAGCATCCAA GGACGGAGAG AAAACAAAGG ACTCTTCCAA GAAGACAGAG 
GAAGTGTCCA AGCCAAAGGC TAAAGAAGAT GCAAGACACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033947.1
CDS	113..2377
Translation	MAFASWWYKTHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSRPHVGEAATATKVTASSAATSKSPSMSTTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	NM_009817.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009817.4

ORF Insert Sequence:

ATGGCATTCG CAAGTTGGTG GTACAAGACG CATGTTAATG AAAAGAATAT TGGATCATCT 
TCTAAACCAG GAGAAAAGAA AGGATCCGAT GAGAAAAAAG CAGCAAGTTT GGGGAGCAGT 
CAGCCTTCCA GACCACATGT TGGTGAAGCT GCCACAGCCA CCAAGGTGAC AGCTTCCTCT 
GCTGCCACCA GCAAGTCCCC CAGCATGAGT ACCACAGAGA CTAAGGCTGT GAAAACAGAG 
TCTAAGAAAC CACAATCAAG TGAGCAACCT GTGGTTCACG AGAAAAAGTC AAAAGGAGGG 
CCAAAGGAAG GGTCAGAGCC CAAAAATCTT CCGAAGCACA CATCAAGTAC AGGAAGCAAG 
CATGCTCATA AAGAAAAAGC ACTTTCCAGA TCAAATGAAC AGATGGTGTC AGAGAAACCA 
TCAGAATCAA AGACAAAATT TCAAGATGTT CCTTCAGCTG GTGGAGAGAG TGTGGCTGGA 
GGTGGGACTG TGGCCACTGC CTTGGACAAA GTGGTTGGTA AGAAAAAAGA ACAGAAACCA 
TTCACACCAG CTTCACCTGT GCAGTCCACA CCGAGCAAGC CATCTGACAA GTCAGGCATG 
GATGCTGCCT TGGATGACCT GATAGACACC TTAGGTGGAC ATGAAGATAC TAACAGAGAT 
GATCCACCAT ATACTGGACC AGTAGTTTTG GACCCAATGT ATTCTACCTA CCTTGAGGCA 
CTGGGTATAA AAGAAGGGAC TATTCCTCCA GAGTATAGGA AACTTTTGGA AAAAAATGAA 
GGGATCACAC AACCTCTTCC AGACTCTCCT AAACCTATGG GGACTGACCA GGCTATAGAT 
GCCTTGTCAT CTGATTTCAC CTGTAGTTCT CCAACTGGAA AGCAAAGTGA GAAAGAGAAG 
TCTACTGGGG AAATTTTCAA AGCTCAGTCT GCAGGAGTGA CCAGAAGTTC TGTTCCCCCC 
AAGGAGAAGA AAAGGAAGGT GGAAGAGGAG GTCATAAATG ATCAAGCACT TCAGGCTCTG 
TCAGATTCAC TGGGCACCCG GCAGCCAGAT CCTCCGAGCC ACGTTAGCCA AGCTGAACAA 
GTCAAAGAGG CAAAAGCAAA AGAAGAAAGG CAGGAGAAGT GTGGTGAAGA TGAGGACACA 
GTCCCAGCTG AGTACAGGTT AAAACCAGCA AAGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAACATCTAA GAGCCTGAGT GAGTCGGAGC TGATTGGGGA GCTTTCAGCA 
GATTTTGACC GATCTACATA TCAAGACAAA CCATCTACGC CAGCTGAAAA GAAATCCAAT 
GACACATCCC AAACTCCTCC GGGGGAGACT GTGCCTCGGG CCTCCATGTG CAGTATACGG 
TCAGCGCCAC CCAAACTAGC ATCCTTGAAG GGCGTGGTAC CAGAAGATGC TGTTGAAACC 
TTGGCTGGAA GCCTGGGGAC AAGGGAAGCA GATCCAGAAC ATGAAAAAAC TGTGGAGGAT 
AAAGTCAAGG AGAAAGCTAA AGAAGAAGAG CATGAAAAAC TTGGTGAAAA AGAAGAAACA 
GTGCCTCCTG ATTATCGACT AGAAGAAGTC AAGGATAAGG ATGGAAAACC ACTCCTGCCC 
AAAGAATCCC AGGAACAACT TGCACCCTTA AGCGATGACT TCCTTCTTGA TGCCTTGTCT 
CAGGACTTCT CCAGTCCGGC AAACATATCG TCTCTTGAAT TTGAAGATGC CAAACTTTCT 
GCTGCCATTT CTGAAGTAGT TTCTCAGACA CCTGCTCCAA GCACCCATGC AGCAGCTCCA 
CTGCCTGGCA CTGAGCAGAA AGACAAAGAA CTTGATGATG CCTTGGATGA ACTTTCTGAC 
AGTCTTGGAC AAAGGCCGCC TGATCCAGAT GAGAACAAAC CACTGGATGA CAAAGTGAAG 
GAGAAAATCA AACCAGAGCA TAGCGAGAAA CTGGGAGAAA GAGACGACAC CATCCCCCCT 
GAATACAGGC ATCTCTTGGA TAATGATGGG AAGGACAAAC CAGAGAAGCC ACCGACTAAG 
AAAACAGAGA AACCTGATCA GGACCGGGAC CCCATTGATG CCCTCTCAGA AGATTTGGAT 
AGCTGCCCCT CAACTACAGA GACCTCAAAG AATACAGCAA AGGGGAAGAG CAAGAAGACT 
TCAAGTTCCA AAGCATCCAA GGACGGAGAG AAAACAAAGG ACTCTTCCAA GAAGACAGAG 
GAAGTGTCCA AGCCAAAGGC TAAAGAAGAT GCAAGACACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_001301155.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288084.1 Mus musculus calpastatin (Cast), transcript variant 3, mRNA.

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CloneID	OMu67313
Clone ID Related Accession (Same CDS sequence)	NM_001301155.1
Accession Version	NM_001301155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	calpastatin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ072297.1, AK029293.1, AK083395.1 and AK037098.1. On Jul 26, 2014 this sequence version replaced XM_006517065.1. Transcript Variant: This variant (3) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (3) with a shorter N-terminus. This variant (3) also uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029293.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTACCA CAGAGACTAA GGCAATTCCA GTAAACAAAC AGTTGGAAGG ACCTGACCAG 
AAAAGACCCA GAGAACAGGC TGTGAAAACA GAGTCTAAGA AACCACAATC AAGTGAGCAA 
CCTGTGGTTC ACGAGAAAAA GTCAAAAGGA GGGCCAAAGG AAGGGTCAGA GCCCAAAAAT 
CTTCCGAAGC ACACATCAAG TACAGGAAGC AAGCATGCTC ATAAAGAAAA AGCACTTTCC 
AGATCAAATG AACAGATGGT GTCAGAGAAA CCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GTTCCTTCAG CTGGTGGAGA GAGTGTGGCT GGAGGTGGGA CTGTGGCCAC TGCCTTGGAC 
AAAGTGGTTG GTAAGAAAAA AGAACAGAAA CCATTCACAC CAGCTTCACC TGTGCAGTCC 
ACACCGAGCA AGCCATCTGA CAAGTCAGGC ATGGATGCTG CCTTGGATGA CCTGATAGAC 
ACCTTAGGTG GACATGAAGA TACTAACAGA GATGATCCAC CATATACTGG ACCAGTAGTT 
TTGGACCCAA TGTATTCTAC CTACCTTGAG GCACTGGGTA TAAAAGAAGG GACTATTCCT 
CCAGAGTATA GGAAACTTTT GGAAAAAAAT GAAGGGATCA CACAACCTCT TCCAGACTCT 
CCTAAACCTA TGGGGACTGA CCAGGCTATA GATGCCTTGT CATCTGATTT CACCTGTAGT 
TCTCCAACTG GAAAGCAAAG TGAGAAAGAG AAGTCTACTG GGGAAATTTT CAAAGCTCAG 
TCTGCAGGAG TGACCAGAAG TTCTGTTCCC CCCAAGGAGA AGAAAAGGAA GGTGGAAGAG 
GAGGTCATAA ATGATCAAGC ACTTCAGGCT CTGTCAGATT CACTGGGCAC CCGGCAGCCA 
GATCCTCCGA GCCACGTTAG CCAAGCTGAA CAAGTCAAAG AGGCAAAAGC AAAAGAAGAA 
AGGCAGGAGA AGTGTGGTGA AGATGAGGAC ACAGTCCCAG CTGAGTACAG GTTAAAACCA 
GCAAAGGATA AAGATGGAAA ACCACTATTG CCAGAGCCTG AAGAAACATC TAAGAGCCTG 
AGTGAGTCGG AGCTGATTGG GGAGCTTTCA GCAGATTTTG ACCGATCTAC ATATCAAGAC 
AAACCATCTA CGCCAGCTGA AAAGAAATCC AATGACACAT CCCAAACTCC TCCGGGGGAG 
ACTGTGCCTC GGGCCTCCAT GTGCAGTATA CGGTCAGCGC CACCCAAACT AGCATCCTTG 
AAGGGCGTGG TACCAGAAGA TGCTGTTGAA ACCTTGGCTG GAAGCCTGGG GACAAGGGAA 
GCAGATCCAG AACATGAAAA AACTGTGGAG GATAAAGTCA AGGAGAAAGC TAAAGAAGAA 
GAGCATGAAA AACTTGGTGA AAAAGAAGAA ACAGTGCCTC CTGATTATCG ACTAGAAGAA 
GTCAAGGATA AGGATGGAAA ACCACTCCTG CCCAAAGAAT CCCAGGAACA ACTTGCACCC 
TTAAGCGATG ACTTCCTTCT TGATGCCTTG TCTCAGGACT TCTCCAGTCC GGCAAACATA 
TCGTCTCTTG AATTTGAAGA TGCCAAACTT TCTGCTGCCA TTTCTGAAGT AGTTTCTCAG 
ACACCTGCTC CAAGCACCCA TGCAGCAGCT CCACTGCCTG GCACTGAGAA AGACAAAGAA 
CTTGATGATG CCTTGGATGA ACTTTCTGAC AGTCTTGGAC AAAGGCCGCC TGATCCAGAT 
GAGAACAAAC CACTGGATGA CAAAGTGAAG GAGAAAATCA AACCAGAGCA TAGCGAGAAA 
CTGGGAGAAA GAGACGACAC CATCCCCCCT GAATACAGGC ATCTCTTGGA TAATGATGGG 
AAGGACAAAC CAGAGAAGCC ACCGACTAAG AAAACAGAGA AACCTGATCA GGACCGGGAC 
CCCATTGATG CCCTCTCAGA AGATTTGGAT AGCTGCCCCT CAACTACAGA GACCTCAAAG 
AATACAGCAA AGGGGAAGAG CAAGAAGACT TCAAGTTCCA AAGCATCCAA GGACGGAGAG 
AAAACAAAGG ACTCTTCCAA GAAGACAGAG GAAGTGTCCA AGCCAAAGGC TAAAGAAGAT 
GCAAGACACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288084.1
CDS	231..2345
Translation	MSTTETKAIPVNKQLEGPDQKRPREQAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	NM_001301155.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301155.1

ORF Insert Sequence:

ATGAGTACCA CAGAGACTAA GGCAATTCCA GTAAACAAAC AGTTGGAAGG ACCTGACCAG 
AAAAGACCCA GAGAACAGGC TGTGAAAACA GAGTCTAAGA AACCACAATC AAGTGAGCAA 
CCTGTGGTTC ACGAGAAAAA GTCAAAAGGA GGGCCAAAGG AAGGGTCAGA GCCCAAAAAT 
CTTCCGAAGC ACACATCAAG TACAGGAAGC AAGCATGCTC ATAAAGAAAA AGCACTTTCC 
AGATCAAATG AACAGATGGT GTCAGAGAAA CCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GTTCCTTCAG CTGGTGGAGA GAGTGTGGCT GGAGGTGGGA CTGTGGCCAC TGCCTTGGAC 
AAAGTGGTTG GTAAGAAAAA AGAACAGAAA CCATTCACAC CAGCTTCACC TGTGCAGTCC 
ACACCGAGCA AGCCATCTGA CAAGTCAGGC ATGGATGCTG CCTTGGATGA CCTGATAGAC 
ACCTTAGGTG GACATGAAGA TACTAACAGA GATGATCCAC CATATACTGG ACCAGTAGTT 
TTGGACCCAA TGTATTCTAC CTACCTTGAG GCACTGGGTA TAAAAGAAGG GACTATTCCT 
CCAGAGTATA GGAAACTTTT GGAAAAAAAT GAAGGGATCA CACAACCTCT TCCAGACTCT 
CCTAAACCTA TGGGGACTGA CCAGGCTATA GATGCCTTGT CATCTGATTT CACCTGTAGT 
TCTCCAACTG GAAAGCAAAG TGAGAAAGAG AAGTCTACTG GGGAAATTTT CAAAGCTCAG 
TCTGCAGGAG TGACCAGAAG TTCTGTTCCC CCCAAGGAGA AGAAAAGGAA GGTGGAAGAG 
GAGGTCATAA ATGATCAAGC ACTTCAGGCT CTGTCAGATT CACTGGGCAC CCGGCAGCCA 
GATCCTCCGA GCCACGTTAG CCAAGCTGAA CAAGTCAAAG AGGCAAAAGC AAAAGAAGAA 
AGGCAGGAGA AGTGTGGTGA AGATGAGGAC ACAGTCCCAG CTGAGTACAG GTTAAAACCA 
GCAAAGGATA AAGATGGAAA ACCACTATTG CCAGAGCCTG AAGAAACATC TAAGAGCCTG 
AGTGAGTCGG AGCTGATTGG GGAGCTTTCA GCAGATTTTG ACCGATCTAC ATATCAAGAC 
AAACCATCTA CGCCAGCTGA AAAGAAATCC AATGACACAT CCCAAACTCC TCCGGGGGAG 
ACTGTGCCTC GGGCCTCCAT GTGCAGTATA CGGTCAGCGC CACCCAAACT AGCATCCTTG 
AAGGGCGTGG TACCAGAAGA TGCTGTTGAA ACCTTGGCTG GAAGCCTGGG GACAAGGGAA 
GCAGATCCAG AACATGAAAA AACTGTGGAG GATAAAGTCA AGGAGAAAGC TAAAGAAGAA 
GAGCATGAAA AACTTGGTGA AAAAGAAGAA ACAGTGCCTC CTGATTATCG ACTAGAAGAA 
GTCAAGGATA AGGATGGAAA ACCACTCCTG CCCAAAGAAT CCCAGGAACA ACTTGCACCC 
TTAAGCGATG ACTTCCTTCT TGATGCCTTG TCTCAGGACT TCTCCAGTCC GGCAAACATA 
TCGTCTCTTG AATTTGAAGA TGCCAAACTT TCTGCTGCCA TTTCTGAAGT AGTTTCTCAG 
ACACCTGCTC CAAGCACCCA TGCAGCAGCT CCACTGCCTG GCACTGAGAA AGACAAAGAA 
CTTGATGATG CCTTGGATGA ACTTTCTGAC AGTCTTGGAC AAAGGCCGCC TGATCCAGAT 
GAGAACAAAC CACTGGATGA CAAAGTGAAG GAGAAAATCA AACCAGAGCA TAGCGAGAAA 
CTGGGAGAAA GAGACGACAC CATCCCCCCT GAATACAGGC ATCTCTTGGA TAATGATGGG 
AAGGACAAAC CAGAGAAGCC ACCGACTAAG AAAACAGAGA AACCTGATCA GGACCGGGAC 
CCCATTGATG CCCTCTCAGA AGATTTGGAT AGCTGCCCCT CAACTACAGA GACCTCAAAG 
AATACAGCAA AGGGGAAGAG CAAGAAGACT TCAAGTTCCA AAGCATCCAA GGACGGAGAG 
AAAACAAAGG ACTCTTCCAA GAAGACAGAG GAAGTGTCCA AGCCAAAGGC TAAAGAAGAT 
GCAAGACACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_001301156.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288085.1 Mus musculus calpastatin (Cast), transcript variant 4, mRNA.

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CloneID	OMu67318
Clone ID Related Accession (Same CDS sequence)	NM_001301156.1
Accession Version	NM_001301156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	calpastatin isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ072297.1, AK083310.1, AK029293.1, AK037098.1 and AK083395.1. On Jul 26, 2014 this sequence version replaced XM_006517066.1. Transcript Variant: This variant (4) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (4) with a shorter N-terminus. This variant (4) also lacks an internal exon in the coding region. The resulting protein (isoform 4) has a shorter N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK083395.1, AK089108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTACCA CAGAGACTAA GGCTGTGAAA ACAGAGTCTA AGAAACCACA ATCAAGTGAG 
CAACCTGTGG TTCACGAGAA AAAGTCAAAA GGAGGGCCAA AGGAAGGGTC AGAGCCCAAA 
AATCTTCCGA AGCACACATC AAGTACAGGA AGCAAGCATG CTCATAAAGA AAAAGCACTT 
TCCAGATCAA ATGAACAGAT GGTGTCAGAG AAACCATCAG AATCAAAGAC AAAATTTCAA 
GATGTTCCTT CAGCTGGTGG AGAGAGTGTG GCTGGAGGTG GGACTGTGGC CACTGCCTTG 
GACAAAGTGG TTGGTAAGAA AAAAGAACAG AAACCATTCA CACCAGCTTC ACCTGTGCAG 
TCCACACCGA GCAAGCCATC TGACAAGTCA GGCATGGATG CTGCCTTGGA TGACCTGATA 
GACACCTTAG GTGGACATGA AGATACTAAC AGAGATGATC CACCATATAC TGGACCAGTA 
GTTTTGGACC CAATGTATTC TACCTACCTT GAGGCACTGG GTATAAAAGA AGGGACTATT 
CCTCCAGAGT ATAGGAAACT TTTGGAAAAA AATGAAGGGA TCACACAACC TCTTCCAGAC 
TCTCCTAAAC CTATGGGGAC TGACCAGGCT ATAGATGCCT TGTCATCTGA TTTCACCTGT 
AGTTCTCCAA CTGGAAAGCA AAGTGAGAAA GAGAAGTCTA CTGGGGAAAT TTTCAAAGCT 
CAGTCTGCAG GAGTGACCAG AAGTTCTGTT CCCCCCAAGG AGAAGAAAAG GAAGGTGGAA 
GAGGAGGTCA TAAATGATCA AGCACTTCAG GCTCTGTCAG ATTCACTGGG CACCCGGCAG 
CCAGATCCTC CGAGCCACGT TAGCCAAGCT GAACAAGTCA AAGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GCAGAAAGAC 
AAAGAACTTG ATGATGCCTT GGATGAACTT TCTGACAGTC TTGGACAAAG GCCGCCTGAT 
CCAGATGAGA ACAAACCACT GGATGACAAA GTGAAGGAGA AAATCAAACC AGAGCATAGC 
GAGAAACTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ACAGGCATCT CTTGGATAAT 
GATGGGAAGG ACAAACCAGA GAAGCCACCG ACTAAGAAAA CAGAGAAACC TGATCAGGAC 
CGGGACCCCA TTGATGCCCT CTCAGAAGAT TTGGATAGCT GCCCCTCAAC TACAGAGACC 
TCAAAGAATA CAGCAAAGGG GAAGAGCAAG AAGACTTCAA GTTCCAAAGC ATCCAAGGAC 
GGAGAGAAAA CAAAGGACTC TTCCAAGAAG ACAGAGGAAG TGTCCAAGCC AAAGGCTAAA 
GAAGATGCAA GACACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288085.1
CDS	231..2291
Translation	MSTTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	NM_001301156.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301156.1

ORF Insert Sequence:

ATGAGTACCA CAGAGACTAA GGCTGTGAAA ACAGAGTCTA AGAAACCACA ATCAAGTGAG 
CAACCTGTGG TTCACGAGAA AAAGTCAAAA GGAGGGCCAA AGGAAGGGTC AGAGCCCAAA 
AATCTTCCGA AGCACACATC AAGTACAGGA AGCAAGCATG CTCATAAAGA AAAAGCACTT 
TCCAGATCAA ATGAACAGAT GGTGTCAGAG AAACCATCAG AATCAAAGAC AAAATTTCAA 
GATGTTCCTT CAGCTGGTGG AGAGAGTGTG GCTGGAGGTG GGACTGTGGC CACTGCCTTG 
GACAAAGTGG TTGGTAAGAA AAAAGAACAG AAACCATTCA CACCAGCTTC ACCTGTGCAG 
TCCACACCGA GCAAGCCATC TGACAAGTCA GGCATGGATG CTGCCTTGGA TGACCTGATA 
GACACCTTAG GTGGACATGA AGATACTAAC AGAGATGATC CACCATATAC TGGACCAGTA 
GTTTTGGACC CAATGTATTC TACCTACCTT GAGGCACTGG GTATAAAAGA AGGGACTATT 
CCTCCAGAGT ATAGGAAACT TTTGGAAAAA AATGAAGGGA TCACACAACC TCTTCCAGAC 
TCTCCTAAAC CTATGGGGAC TGACCAGGCT ATAGATGCCT TGTCATCTGA TTTCACCTGT 
AGTTCTCCAA CTGGAAAGCA AAGTGAGAAA GAGAAGTCTA CTGGGGAAAT TTTCAAAGCT 
CAGTCTGCAG GAGTGACCAG AAGTTCTGTT CCCCCCAAGG AGAAGAAAAG GAAGGTGGAA 
GAGGAGGTCA TAAATGATCA AGCACTTCAG GCTCTGTCAG ATTCACTGGG CACCCGGCAG 
CCAGATCCTC CGAGCCACGT TAGCCAAGCT GAACAAGTCA AAGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GCAGAAAGAC 
AAAGAACTTG ATGATGCCTT GGATGAACTT TCTGACAGTC TTGGACAAAG GCCGCCTGAT 
CCAGATGAGA ACAAACCACT GGATGACAAA GTGAAGGAGA AAATCAAACC AGAGCATAGC 
GAGAAACTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ACAGGCATCT CTTGGATAAT 
GATGGGAAGG ACAAACCAGA GAAGCCACCG ACTAAGAAAA CAGAGAAACC TGATCAGGAC 
CGGGACCCCA TTGATGCCCT CTCAGAAGAT TTGGATAGCT GCCCCTCAAC TACAGAGACC 
TCAAAGAATA CAGCAAAGGG GAAGAGCAAG AAGACTTCAA GTTCCAAAGC ATCCAAGGAC 
GGAGAGAAAA CAAAGGACTC TTCCAAGAAG ACAGAGGAAG TGTCCAAGCC AAAGGCTAAA 
GAAGATGCAA GACACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( NM_001301153.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288082.1 Mus musculus calpastatin (Cast), transcript variant 1, mRNA.

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CloneID	OMu67319
Clone ID Related Accession (Same CDS sequence)	NM_001301153.1
Accession Version	NM_001301153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	calpastatin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY771015.1, AA033475.1, CF164079.1, CN529927.1, AK083395.1, AK036576.1 and AK037098.1. On Jul 26, 2014 this sequence version replaced XM_006517055.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB026997.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCCGAA GCCTGCCGCC TCTCCCAGGC CCAGTCGCGG CGCGGCAGCT 
CGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGGAGAA 
AAGAAAGGAT CCGATGAGAA AAAAGCAGCA AGTTTGGGGA GCAGTCAGCC TTCCAGACCA 
CATGTTGGTG AAGCTGCCAC AGCCACCAAG GTGACAGCTT CCTCTGCTGC CACCAGCAAG 
TCCCCCAGCA TGAGTACCAC AGAGACTAAG GCAATTCCAG TAAACAAACA GTTGGAAGGA 
CCTGACCAGA AAAGACCCAG AGAACAGGCT GTGAAAACAG AGTCTAAGAA ACCACAATCA 
AGTGAGCAAC CTGTGGTTCA CGAGAAAAAG TCAAAAGGAG GGCCAAAGGA AGGGTCAGAG 
CCCAAAAATC TTCCGAAGCA CACATCAAGT ACAGGAAGCA AGCATGCTCA TAAAGAAAAA 
GCACTTTCCA GATCAAATGA ACAGATGGTG TCAGAGAAAC CATCAGAATC AAAGACAAAA 
TTTCAAGATG TTCCTTCAGC TGGTGGAGAG AGTGTGGCTG GAGGTGGGAC TGTGGCCACT 
GCCTTGGACA AAGTGGTTGG TAAGAAAAAA GAACAGAAAC CATTCACACC AGCTTCACCT 
GTGCAGTCCA CACCGAGCAA GCCATCTGAC AAGTCAGGCA TGGATGCTGC CTTGGATGAC 
CTGATAGACA CCTTAGGTGG ACATGAAGAT ACTAACAGAG ATGATCCACC ATATACTGGA 
CCAGTAGTTT TGGACCCAAT GTATTCTACC TACCTTGAGG CACTGGGTAT AAAAGAAGGG 
ACTATTCCTC CAGAGTATAG GAAACTTTTG GAAAAAAATG AAGGGATCAC ACAACCTCTT 
CCAGACTCTC CTAAACCTAT GGGGACTGAC CAGGCTATAG ATGCCTTGTC ATCTGATTTC 
ACCTGTAGTT CTCCAACTGG AAAGCAAAGT GAGAAAGAGA AGTCTACTGG GGAAATTTTC 
AAAGCTCAGT CTGCAGGAGT GACCAGAAGT TCTGTTCCCC CCAAGGAGAA GAAAAGGAAG 
GTGGAAGAGG AGGTCATAAA TGATCAAGCA CTTCAGGCTC TGTCAGATTC ACTGGGCACC 
CGGCAGCCAG ATCCTCCGAG CCACGTTAGC CAAGCTGAAC AAGTCAAAGA GGCAAAAGCA 
AAAGAAGAAA GGCAGGAGAA GTGTGGTGAA GATGAGGACA CAGTCCCAGC TGAGTACAGG 
TTAAAACCAG CAAAGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAACATCT 
AAGAGCCTGA GTGAGTCGGA GCTGATTGGG GAGCTTTCAG CAGATTTTGA CCGATCTACA 
TATCAAGACA AACCATCTAC GCCAGCTGAA AAGAAATCCA ATGACACATC CCAAACTCCT 
CCGGGGGAGA CTGTGCCTCG GGCCTCCATG TGCAGTATAC GGTCAGCGCC ACCCAAACTA 
GCATCCTTGA AGGGCGTGGT ACCAGAAGAT GCTGTTGAAA CCTTGGCTGG AAGCCTGGGG 
ACAAGGGAAG CAGATCCAGA ACATGAAAAA ACTGTGGAGG ATAAAGTCAA GGAGAAAGCT 
AAAGAAGAAG AGCATGAAAA ACTTGGTGAA AAAGAAGAAA CAGTGCCTCC TGATTATCGA 
CTAGAAGAAG TCAAGGATAA GGATGGAAAA CCACTCCTGC CCAAAGAATC CCAGGAACAA 
CTTGCACCCT TAAGCGATGA CTTCCTTCTT GATGCCTTGT CTCAGGACTT CTCCAGTCCG 
GCAAACATAT CGTCTCTTGA ATTTGAAGAT GCCAAACTTT CTGCTGCCAT TTCTGAAGTA 
GTTTCTCAGA CACCTGCTCC AAGCACCCAT GCAGCAGCTC CACTGCCTGG CACTGAGCAG 
AAAGACAAAG AACTTGATGA TGCCTTGGAT GAACTTTCTG ACAGTCTTGG ACAAAGGCCG 
CCTGATCCAG ATGAGAACAA ACCACTGGAT GACAAAGTGA AGGAGAAAAT CAAACCAGAG 
CATAGCGAGA AACTGGGAGA AAGAGACGAC ACCATCCCCC CTGAATACAG GCATCTCTTG 
GATAATGATG GGAAGGACAA ACCAGAGAAG CCACCGACTA AGAAAACAGA GAAACCTGAT 
CAGGACCGGG ACCCCATTGA TGCCCTCTCA GAAGATTTGG ATAGCTGCCC CTCAACTACA 
GAGACCTCAA AGAATACAGC AAAGGGGAAG AGCAAGAAGA CTTCAAGTTC CAAAGCATCC 
AAGGACGGAG AGAAAACAAA GGACTCTTCC AAGAAGACAG AGGAAGTGTC CAAGCCAAAG 
GCTAAAGAAG ATGCAAGACA CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288082.1
CDS	251..2617
Translation	MSQPGPKPAASPRPSRGAAARHTQEHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSRPHVGEAATATKVTASSAATSKSPSMSTTETKAIPVNKQLEGPDQKRPREQAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	NM_001301153.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301153.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCCGAA GCCTGCCGCC TCTCCCAGGC CCAGTCGCGG CGCGGCAGCT 
CGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGGAGAA 
AAGAAAGGAT CCGATGAGAA AAAAGCAGCA AGTTTGGGGA GCAGTCAGCC TTCCAGACCA 
CATGTTGGTG AAGCTGCCAC AGCCACCAAG GTGACAGCTT CCTCTGCTGC CACCAGCAAG 
TCCCCCAGCA TGAGTACCAC AGAGACTAAG GCAATTCCAG TAAACAAACA GTTGGAAGGA 
CCTGACCAGA AAAGACCCAG AGAACAGGCT GTGAAAACAG AGTCTAAGAA ACCACAATCA 
AGTGAGCAAC CTGTGGTTCA CGAGAAAAAG TCAAAAGGAG GGCCAAAGGA AGGGTCAGAG 
CCCAAAAATC TTCCGAAGCA CACATCAAGT ACAGGAAGCA AGCATGCTCA TAAAGAAAAA 
GCACTTTCCA GATCAAATGA ACAGATGGTG TCAGAGAAAC CATCAGAATC AAAGACAAAA 
TTTCAAGATG TTCCTTCAGC TGGTGGAGAG AGTGTGGCTG GAGGTGGGAC TGTGGCCACT 
GCCTTGGACA AAGTGGTTGG TAAGAAAAAA GAACAGAAAC CATTCACACC AGCTTCACCT 
GTGCAGTCCA CACCGAGCAA GCCATCTGAC AAGTCAGGCA TGGATGCTGC CTTGGATGAC 
CTGATAGACA CCTTAGGTGG ACATGAAGAT ACTAACAGAG ATGATCCACC ATATACTGGA 
CCAGTAGTTT TGGACCCAAT GTATTCTACC TACCTTGAGG CACTGGGTAT AAAAGAAGGG 
ACTATTCCTC CAGAGTATAG GAAACTTTTG GAAAAAAATG AAGGGATCAC ACAACCTCTT 
CCAGACTCTC CTAAACCTAT GGGGACTGAC CAGGCTATAG ATGCCTTGTC ATCTGATTTC 
ACCTGTAGTT CTCCAACTGG AAAGCAAAGT GAGAAAGAGA AGTCTACTGG GGAAATTTTC 
AAAGCTCAGT CTGCAGGAGT GACCAGAAGT TCTGTTCCCC CCAAGGAGAA GAAAAGGAAG 
GTGGAAGAGG AGGTCATAAA TGATCAAGCA CTTCAGGCTC TGTCAGATTC ACTGGGCACC 
CGGCAGCCAG ATCCTCCGAG CCACGTTAGC CAAGCTGAAC AAGTCAAAGA GGCAAAAGCA 
AAAGAAGAAA GGCAGGAGAA GTGTGGTGAA GATGAGGACA CAGTCCCAGC TGAGTACAGG 
TTAAAACCAG CAAAGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAACATCT 
AAGAGCCTGA GTGAGTCGGA GCTGATTGGG GAGCTTTCAG CAGATTTTGA CCGATCTACA 
TATCAAGACA AACCATCTAC GCCAGCTGAA AAGAAATCCA ATGACACATC CCAAACTCCT 
CCGGGGGAGA CTGTGCCTCG GGCCTCCATG TGCAGTATAC GGTCAGCGCC ACCCAAACTA 
GCATCCTTGA AGGGCGTGGT ACCAGAAGAT GCTGTTGAAA CCTTGGCTGG AAGCCTGGGG 
ACAAGGGAAG CAGATCCAGA ACATGAAAAA ACTGTGGAGG ATAAAGTCAA GGAGAAAGCT 
AAAGAAGAAG AGCATGAAAA ACTTGGTGAA AAAGAAGAAA CAGTGCCTCC TGATTATCGA 
CTAGAAGAAG TCAAGGATAA GGATGGAAAA CCACTCCTGC CCAAAGAATC CCAGGAACAA 
CTTGCACCCT TAAGCGATGA CTTCCTTCTT GATGCCTTGT CTCAGGACTT CTCCAGTCCG 
GCAAACATAT CGTCTCTTGA ATTTGAAGAT GCCAAACTTT CTGCTGCCAT TTCTGAAGTA 
GTTTCTCAGA CACCTGCTCC AAGCACCCAT GCAGCAGCTC CACTGCCTGG CACTGAGCAG 
AAAGACAAAG AACTTGATGA TGCCTTGGAT GAACTTTCTG ACAGTCTTGG ACAAAGGCCG 
CCTGATCCAG ATGAGAACAA ACCACTGGAT GACAAAGTGA AGGAGAAAAT CAAACCAGAG 
CATAGCGAGA AACTGGGAGA AAGAGACGAC ACCATCCCCC CTGAATACAG GCATCTCTTG 
GATAATGATG GGAAGGACAA ACCAGAGAAG CCACCGACTA AGAAAACAGA GAAACCTGAT 
CAGGACCGGG ACCCCATTGA TGCCCTCTCA GAAGATTTGG ATAGCTGCCC CTCAACTACA 
GAGACCTCAA AGAATACAGC AAAGGGGAAG AGCAAGAAGA CTTCAAGTTC CAAAGCATCC 
AAGGACGGAG AGAAAACAAA GGACTCTTCC AAGAAGACAG AGGAAGTGTC CAAGCCAAAG 
GCTAAAGAAG ATGCAAGACA CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_006517056.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517119.1 Mus musculus calpastatin (Cast), transcript variant X1, mRNA.

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CloneID	OMu48635
Clone ID Related Accession (Same CDS sequence)	XM_006517056.2
Accession Version	XM_006517056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006517056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCCGAA GCCTGCCGCC TCTCCCAGGC CCAGTCGCGG CGCGGCAGCT 
CGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGGAGAA 
AAGAAAGGAT CCGATGAGAA AAAAGCAGCA AGTTTGGGGA GCAGTCAGCC TTCCAGACCA 
CATGTTGGTG AAGCTGCCAC AGCCACCAAG GTGACAGCTT CCTCTGCTGC CACCAGCAAG 
TCCCCCAGCA TGAGTACCAC AGAGACTAAG GCAATTCCAG TAAACAAACA GTTGGAAGGA 
CCTGACCAGA AAAGACCCAG AGAACAGGCT GTGAAAACAG AGTCTAAGAA ACCACAATCA 
AGTGAGCAAC CTGTGGTTCA CGAGAAAAAG TCAAAAGGAG GGCCAAAGGA AGGGTCAGAG 
CCCAAAAATC TTCCGAAGCA CACATCAAGT ACAGGAAGCA AGCATGCTCA TAAAGAAAAA 
GCACTTTCCA GATCAAATGA ACAGATGGTG TCAGAGAAAC CATCAGAATC AAAGACAAAA 
TTTCAAGATG TTCCTTCAGC TGGTGGAGAG AGTGTGGCTG GAGGTGGGAC TGTGGCCACT 
GCCTTGGACA AAGTGGTTGG TAAGAAAAAA GAACAGAAAC CATTCACACC AGCTTCACCT 
GTGCAGTCCA CACCGAGCAA GCCATCTGAC AAGTCAGGCA TGGATGCTGC CTTGGATGAC 
CTGATAGACA CCTTAGGTGG ACATGAAGAT ACTAACAGAG ATGATCCACC ATATACTGGA 
CCAGTAGTTT TGGACCCAAT GTATTCTACC TACCTTGAGG CACTGGGTAT AAAAGAAGGG 
ACTATTCCTC CAGAGTATAG GAAACTTTTG GAAAAAAATG AAGGGATCAC ACAACCTCTT 
CCAGACTCTC CTAAACCTAT GGGGACTGAC CAGGCTATAG ATGCCTTGTC ATCTGATTTC 
ACCTGTAGTT CTCCAACTGG AAAGCAAAGT GAGAAAGAGA AGTCTACTGG GGAAATTTTC 
AAAGCTCAGT CTGCAGGAGT GACCAGAAGT TCTGTTCCCC CCAAGGAGAA GAAAAGGAAG 
GTGGAAGAGG AGGTCATAAA TGATCAAGCA CTTCAGGCTC TGTCAGATTC ACTGGGCACC 
CGGCAGCCAG ATCCTCCGAG CCACGTTAGC CAAGCTGAAC AAGTCAAAGA GGCAAAAGCA 
AAAGAAGAAA GGCAGGAGAA GTGTGGTGAA GATGAGGACA CAGTCCCAGC TGAGTACAGG 
TTAAAACCAG CAAAGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAACATCT 
AAGAGCCTGA GTGAGTCGGA GCTGATTGGG GAGCTTTCAG CAGATTTTGA CCGATCTACA 
TATCAAGACA AACCATCTAC GCCAGCTGAA AAGAAATCCA ATGACACATC CCAAACTCCT 
CCGGGGGAGA CTGTGCCTCG GGCCTCCATG TGCAGTATAC GGTCAGCGCC ACCCAAACTA 
GCATCCTTGA AGGGCGTGGT ACCAGAAGAT GCTGTTGAAA CCTTGGCTGG AAGCCTGGGG 
ACAAGGGAAG CAGATCCAGA ACATGAAAAA ACTGTGGAGG ATAAAGTCAA GGAGAAAGCT 
AAAGAAGAAG AGCATGAAAA ACTTGGTGAA AAAGAAGAAA CAGTGCCTCC TGATTATCGA 
CTAGAAGAAG TCAAGGATAA GGATGGAAAA CCACTCCTGC CCAAAGAATC CCAGGAACAA 
CTTGCACCCT TAAGCGATGA CTTCCTTCTT GATGCCTTGT CTCAGGACTT CTCCAGTCCG 
GCAAACATAT CGTCTCTTGA ATTTGAAGAT GCCAAACTTT CTGCTGCCAT TTCTGAAGTA 
GTTTCTCAGA CACCTGCTCC AAGCACCCAT GCAGCAGCTC CACTGCCTGG CACTGAGAAA 
GACAAAGAAC TTGATGATGC CTTGGATGAA CTTTCTGACA GTCTTGGACA AAGGCCGCCT 
GATCCAGATG AGAACAAACC ACTGGATGAC AAAGTGAAGG AGAAAATCAA ACCAGAGCAT 
AGCGAGAAAC TGGGAGAAAG AGACGACACC ATCCCCCCTG AATACAGGCA TCTCTTGGAT 
AATGATGGGA AGGACAAACC AGAGAAGCCA CCGACTAAGA AAACAGAGAA ACCTGATCAG 
GACCGGGACC CCATTGATGC CCTCTCAGAA GATTTGGATA GCTGCCCCTC AACTACAGAG 
ACCTCAAAGA ATACAGCAAA GGGGAAGAGC AAGAAGACTT CAAGTTCCAA AGCATCCAAG 
GACGGAGAGA AAACAAAGGA CTCTTCCAAG AAGACAGAGG AAGTGTCCAA GCCAAAGGCT 
AAAGAAGATG CAAGACACAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517119.1
CDS	365..2728
Translation	MSQPGPKPAASPRPSRGAAARHTQEHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSRPHVGEAATATKVTASSAATSKSPSMSTTETKAIPVNKQLEGPDQKRPREQAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_006517056.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517056.2

ORF Insert Sequence:

ATGTCCCAGC CCGGCCCGAA GCCTGCCGCC TCTCCCAGGC CCAGTCGCGG CGCGGCAGCT 
CGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGGAGAA 
AAGAAAGGAT CCGATGAGAA AAAAGCAGCA AGTTTGGGGA GCAGTCAGCC TTCCAGACCA 
CATGTTGGTG AAGCTGCCAC AGCCACCAAG GTGACAGCTT CCTCTGCTGC CACCAGCAAG 
TCCCCCAGCA TGAGTACCAC AGAGACTAAG GCAATTCCAG TAAACAAACA GTTGGAAGGA 
CCTGACCAGA AAAGACCCAG AGAACAGGCT GTGAAAACAG AGTCTAAGAA ACCACAATCA 
AGTGAGCAAC CTGTGGTTCA CGAGAAAAAG TCAAAAGGAG GGCCAAAGGA AGGGTCAGAG 
CCCAAAAATC TTCCGAAGCA CACATCAAGT ACAGGAAGCA AGCATGCTCA TAAAGAAAAA 
GCACTTTCCA GATCAAATGA ACAGATGGTG TCAGAGAAAC CATCAGAATC AAAGACAAAA 
TTTCAAGATG TTCCTTCAGC TGGTGGAGAG AGTGTGGCTG GAGGTGGGAC TGTGGCCACT 
GCCTTGGACA AAGTGGTTGG TAAGAAAAAA GAACAGAAAC CATTCACACC AGCTTCACCT 
GTGCAGTCCA CACCGAGCAA GCCATCTGAC AAGTCAGGCA TGGATGCTGC CTTGGATGAC 
CTGATAGACA CCTTAGGTGG ACATGAAGAT ACTAACAGAG ATGATCCACC ATATACTGGA 
CCAGTAGTTT TGGACCCAAT GTATTCTACC TACCTTGAGG CACTGGGTAT AAAAGAAGGG 
ACTATTCCTC CAGAGTATAG GAAACTTTTG GAAAAAAATG AAGGGATCAC ACAACCTCTT 
CCAGACTCTC CTAAACCTAT GGGGACTGAC CAGGCTATAG ATGCCTTGTC ATCTGATTTC 
ACCTGTAGTT CTCCAACTGG AAAGCAAAGT GAGAAAGAGA AGTCTACTGG GGAAATTTTC 
AAAGCTCAGT CTGCAGGAGT GACCAGAAGT TCTGTTCCCC CCAAGGAGAA GAAAAGGAAG 
GTGGAAGAGG AGGTCATAAA TGATCAAGCA CTTCAGGCTC TGTCAGATTC ACTGGGCACC 
CGGCAGCCAG ATCCTCCGAG CCACGTTAGC CAAGCTGAAC AAGTCAAAGA GGCAAAAGCA 
AAAGAAGAAA GGCAGGAGAA GTGTGGTGAA GATGAGGACA CAGTCCCAGC TGAGTACAGG 
TTAAAACCAG CAAAGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAACATCT 
AAGAGCCTGA GTGAGTCGGA GCTGATTGGG GAGCTTTCAG CAGATTTTGA CCGATCTACA 
TATCAAGACA AACCATCTAC GCCAGCTGAA AAGAAATCCA ATGACACATC CCAAACTCCT 
CCGGGGGAGA CTGTGCCTCG GGCCTCCATG TGCAGTATAC GGTCAGCGCC ACCCAAACTA 
GCATCCTTGA AGGGCGTGGT ACCAGAAGAT GCTGTTGAAA CCTTGGCTGG AAGCCTGGGG 
ACAAGGGAAG CAGATCCAGA ACATGAAAAA ACTGTGGAGG ATAAAGTCAA GGAGAAAGCT 
AAAGAAGAAG AGCATGAAAA ACTTGGTGAA AAAGAAGAAA CAGTGCCTCC TGATTATCGA 
CTAGAAGAAG TCAAGGATAA GGATGGAAAA CCACTCCTGC CCAAAGAATC CCAGGAACAA 
CTTGCACCCT TAAGCGATGA CTTCCTTCTT GATGCCTTGT CTCAGGACTT CTCCAGTCCG 
GCAAACATAT CGTCTCTTGA ATTTGAAGAT GCCAAACTTT CTGCTGCCAT TTCTGAAGTA 
GTTTCTCAGA CACCTGCTCC AAGCACCCAT GCAGCAGCTC CACTGCCTGG CACTGAGAAA 
GACAAAGAAC TTGATGATGC CTTGGATGAA CTTTCTGACA GTCTTGGACA AAGGCCGCCT 
GATCCAGATG AGAACAAACC ACTGGATGAC AAAGTGAAGG AGAAAATCAA ACCAGAGCAT 
AGCGAGAAAC TGGGAGAAAG AGACGACACC ATCCCCCCTG AATACAGGCA TCTCTTGGAT 
AATGATGGGA AGGACAAACC AGAGAAGCCA CCGACTAAGA AAACAGAGAA ACCTGATCAG 
GACCGGGACC CCATTGATGC CCTCTCAGAA GATTTGGATA GCTGCCCCTC AACTACAGAG 
ACCTCAAAGA ATACAGCAAA GGGGAAGAGC AAGAAGACTT CAAGTTCCAA AGCATCCAAG 
GACGGAGAGA AAACAAAGGA CTCTTCCAAG AAGACAGAGG AAGTGTCCAA GCCAAAGGCT 
AAAGAAGATG CAAGACACAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_006517059.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517122.1 Mus musculus calpastatin (Cast), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu48638
Clone ID Related Accession (Same CDS sequence)	XM_006517059.1
Accession Version	XM_006517059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCCGAA GCCTGCCGCC TCTCCCAGGC CCAGTCGCGG CGCGGCAGCT 
CGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGGAGAA 
AAGAAAGGAT CCGATGAGAA AAAAGCAGCA AGTTTGGGGA GCAGTCAGCC TTCCAGACCA 
CATGTTGGTG AAGCTGCCAC AGCCACCAAG GTGACAGCTT CCTCTGCTGC CACCAGCAAG 
TCCCCCAGCA TGAGTACCAC AGAGACTAAG GCTGTGAAAA CAGAGTCTAA GAAACCACAA 
TCAAGTGAGC AACCTGTGGT TCACGAGAAA AAGTCAAAAG GAGGGCCAAA GGAAGGGTCA 
GAGCCCAAAA ATCTTCCGAA GCACACATCA AGTACAGGAA GCAAGCATGC TCATAAAGAA 
AAAGCACTTT CCAGATCAAA TGAACAGATG GTGTCAGAGA AACCATCAGA ATCAAAGACA 
AAATTTCAAG ATGTTCCTTC AGCTGGTGGA GAGAGTGTGG CTGGAGGTGG GACTGTGGCC 
ACTGCCTTGG ACAAAGTGGT TGGTAAGAAA AAAGAACAGA AACCATTCAC ACCAGCTTCA 
CCTGTGCAGT CCACACCGAG CAAGCCATCT GACAAGTCAG GCATGGATGC TGCCTTGGAT 
GACCTGATAG ACACCTTAGG TGGACATGAA GATACTAACA GAGATGATCC ACCATATACT 
GGACCAGTAG TTTTGGACCC AATGTATTCT ACCTACCTTG AGGCACTGGG TATAAAAGAA 
GGGACTATTC CTCCAGAGTA TAGGAAACTT TTGGAAAAAA ATGAAGGGAT CACACAACCT 
CTTCCAGACT CTCCTAAACC TATGGGGACT GACCAGGCTA TAGATGCCTT GTCATCTGAT 
TTCACCTGTA GTTCTCCAAC TGGAAAGCAA AGTGAGAAAG AGAAGTCTAC TGGGGAAATT 
TTCAAAGCTC AGTCTGCAGG AGTGACCAGA AGTTCTGTTC CCCCCAAGGA GAAGAAAAGG 
AAGGTGGAAG AGGAGGTCAT AAATGATCAA GCACTTCAGG CTCTGTCAGA TTCACTGGGC 
ACCCGGCAGC CAGATCCTCC GAGCCACGTT AGCCAAGCTG AACAAGTCAA AGAGGCAAAA 
GCAAAAGAAG AAAGGCAGGA GAAGTGTGGT GAAGATGAGG ACACAGTCCC AGCTGAGTAC 
AGGTTAAAAC CAGCAAAGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAACA 
TCTAAGAGCC TGAGTGAGTC GGAGCTGATT GGGGAGCTTT CAGCAGATTT TGACCGATCT 
ACATATCAAG ACAAACCATC TACGCCAGCT GAAAAGAAAT CCAATGACAC ATCCCAAACT 
CCTCCGGGGG AGACTGTGCC TCGGGCCTCC ATGTGCAGTA TACGGTCAGC GCCACCCAAA 
CTAGCATCCT TGAAGGGCGT GGTACCAGAA GATGCTGTTG AAACCTTGGC TGGAAGCCTG 
GGGACAAGGG AAGCAGATCC AGAACATGAA AAAACTGTGG AGGATAAAGT CAAGGAGAAA 
GCTAAAGAAG AAGAGCATGA AAAACTTGGT GAAAAAGAAG AAACAGTGCC TCCTGATTAT 
CGACTAGAAG AAGTCAAGGA TAAGGATGGA AAACCACTCC TGCCCAAAGA ATCCCAGGAA 
CAACTTGCAC CCTTAAGCGA TGACTTCCTT CTTGATGCCT TGTCTCAGGA CTTCTCCAGT 
CCGGCAAACA TATCGTCTCT TGAATTTGAA GATGCCAAAC TTTCTGCTGC CATTTCTGAA 
GTAGTTTCTC AGACACCTGC TCCAAGCACC CATGCAGCAG CTCCACTGCC TGGCACTGAG 
CAGAAAGACA AAGAACTTGA TGATGCCTTG GATGAACTTT CTGACAGTCT TGGACAAAGG 
CCGCCTGATC CAGATGAGAA CAAACCACTG GATGACAAAG TGAAGGAGAA AATCAAACCA 
GAGCATAGCG AGAAACTGGG AGAAAGAGAC GACACCATCC CCCCTGAATA CAGGCATCTC 
TTGGATAATG ATGGGAAGGA CAAACCAGAG AAGCCACCGA CTAAGAAAAC AGAGAAACCT 
GATCAGGACC GGGACCCCAT TGATGCCCTC TCAGAAGATT TGGATAGCTG CCCCTCAACT 
ACAGAGACCT CAAAGAATAC AGCAAAGGGG AAGAGCAAGA AGACTTCAAG TTCCAAAGCA 
TCCAAGGACG GAGAGAAAAC AAAGGACTCT TCCAAGAAGA CAGAGGAAGT GTCCAAGCCA 
AAGGCTAAAG AAGATGCAAG ACACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517122.1
CDS	365..2674
Translation	MSQPGPKPAASPRPSRGAAARHTQEHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSRPHVGEAATATKVTASSAATSKSPSMSTTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_006517059.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517059.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCCGAA GCCTGCCGCC TCTCCCAGGC CCAGTCGCGG CGCGGCAGCT 
CGCCACACCC AGGAGCATGT TAATGAAAAG AATATTGGAT CATCTTCTAA ACCAGGAGAA 
AAGAAAGGAT CCGATGAGAA AAAAGCAGCA AGTTTGGGGA GCAGTCAGCC TTCCAGACCA 
CATGTTGGTG AAGCTGCCAC AGCCACCAAG GTGACAGCTT CCTCTGCTGC CACCAGCAAG 
TCCCCCAGCA TGAGTACCAC AGAGACTAAG GCTGTGAAAA CAGAGTCTAA GAAACCACAA 
TCAAGTGAGC AACCTGTGGT TCACGAGAAA AAGTCAAAAG GAGGGCCAAA GGAAGGGTCA 
GAGCCCAAAA ATCTTCCGAA GCACACATCA AGTACAGGAA GCAAGCATGC TCATAAAGAA 
AAAGCACTTT CCAGATCAAA TGAACAGATG GTGTCAGAGA AACCATCAGA ATCAAAGACA 
AAATTTCAAG ATGTTCCTTC AGCTGGTGGA GAGAGTGTGG CTGGAGGTGG GACTGTGGCC 
ACTGCCTTGG ACAAAGTGGT TGGTAAGAAA AAAGAACAGA AACCATTCAC ACCAGCTTCA 
CCTGTGCAGT CCACACCGAG CAAGCCATCT GACAAGTCAG GCATGGATGC TGCCTTGGAT 
GACCTGATAG ACACCTTAGG TGGACATGAA GATACTAACA GAGATGATCC ACCATATACT 
GGACCAGTAG TTTTGGACCC AATGTATTCT ACCTACCTTG AGGCACTGGG TATAAAAGAA 
GGGACTATTC CTCCAGAGTA TAGGAAACTT TTGGAAAAAA ATGAAGGGAT CACACAACCT 
CTTCCAGACT CTCCTAAACC TATGGGGACT GACCAGGCTA TAGATGCCTT GTCATCTGAT 
TTCACCTGTA GTTCTCCAAC TGGAAAGCAA AGTGAGAAAG AGAAGTCTAC TGGGGAAATT 
TTCAAAGCTC AGTCTGCAGG AGTGACCAGA AGTTCTGTTC CCCCCAAGGA GAAGAAAAGG 
AAGGTGGAAG AGGAGGTCAT AAATGATCAA GCACTTCAGG CTCTGTCAGA TTCACTGGGC 
ACCCGGCAGC CAGATCCTCC GAGCCACGTT AGCCAAGCTG AACAAGTCAA AGAGGCAAAA 
GCAAAAGAAG AAAGGCAGGA GAAGTGTGGT GAAGATGAGG ACACAGTCCC AGCTGAGTAC 
AGGTTAAAAC CAGCAAAGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAACA 
TCTAAGAGCC TGAGTGAGTC GGAGCTGATT GGGGAGCTTT CAGCAGATTT TGACCGATCT 
ACATATCAAG ACAAACCATC TACGCCAGCT GAAAAGAAAT CCAATGACAC ATCCCAAACT 
CCTCCGGGGG AGACTGTGCC TCGGGCCTCC ATGTGCAGTA TACGGTCAGC GCCACCCAAA 
CTAGCATCCT TGAAGGGCGT GGTACCAGAA GATGCTGTTG AAACCTTGGC TGGAAGCCTG 
GGGACAAGGG AAGCAGATCC AGAACATGAA AAAACTGTGG AGGATAAAGT CAAGGAGAAA 
GCTAAAGAAG AAGAGCATGA AAAACTTGGT GAAAAAGAAG AAACAGTGCC TCCTGATTAT 
CGACTAGAAG AAGTCAAGGA TAAGGATGGA AAACCACTCC TGCCCAAAGA ATCCCAGGAA 
CAACTTGCAC CCTTAAGCGA TGACTTCCTT CTTGATGCCT TGTCTCAGGA CTTCTCCAGT 
CCGGCAAACA TATCGTCTCT TGAATTTGAA GATGCCAAAC TTTCTGCTGC CATTTCTGAA 
GTAGTTTCTC AGACACCTGC TCCAAGCACC CATGCAGCAG CTCCACTGCC TGGCACTGAG 
CAGAAAGACA AAGAACTTGA TGATGCCTTG GATGAACTTT CTGACAGTCT TGGACAAAGG 
CCGCCTGATC CAGATGAGAA CAAACCACTG GATGACAAAG TGAAGGAGAA AATCAAACCA 
GAGCATAGCG AGAAACTGGG AGAAAGAGAC GACACCATCC CCCCTGAATA CAGGCATCTC 
TTGGATAATG ATGGGAAGGA CAAACCAGAG AAGCCACCGA CTAAGAAAAC AGAGAAACCT 
GATCAGGACC GGGACCCCAT TGATGCCCTC TCAGAAGATT TGGATAGCTG CCCCTCAACT 
ACAGAGACCT CAAAGAATAC AGCAAAGGGG AAGAGCAAGA AGACTTCAAG TTCCAAAGCA 
TCCAAGGACG GAGAGAAAAC AAAGGACTCT TCCAAGAAGA CAGAGGAAGT GTCCAAGCCA 
AAGGCTAAAG AAGATGCAAG ACACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_011244466.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242768.1 Mus musculus calpastatin (Cast), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67309
Clone ID Related Accession (Same CDS sequence)	XM_011244466.2 , XM_011244466.3
Accession Version	XM_011244466.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CAAGTTGGTG GTACAAGACG CATGTTAATG AAAAGAATAT TGGATCATCT 
TCTAAACCAG GAGAAAAGAA AGGATCCGAT GAGAAAAAAG CAGCAAGTTT GGGGAGCAGT 
CAGCCTTCCA GACCACATGT TGGTGAAGCT GCCACAGCCA CCAAGGTGAC AGCTTCCTCT 
GCTGCCACCA GCAAGTCCCC CAGCATGAGT ACCACAGAGA CTAAGGCAAT TCCAGTAAAC 
AAACAGTTGG AAGGACCTGA CCAGAAAAGA CCCAGAGAAC AGGCTGTGAA AACAGAGTCT 
AAGAAACCAC AATCAAGTGA GCAACCTGTG GTTCACGAGA AAAAGTCAAA AGGAGGGCCA 
AAGGAAGGGT CAGAGCCCAA AAATCTTCCG AAGCACACAT CAAGTACAGG AAGCAAGCAT 
GCTCATAAAG AAAAAGCACT TTCCAGATCA AATGAACAGA TGGTGTCAGA GAAACCATCA 
GAATCAAAGA CAAAATTTCA AGATGTTCCT TCAGCTGGTG GAGAGAGTGT GGCTGGAGGT 
GGGACTGTGG CCACTGCCTT GGACAAAGTG GTTGGTAAGA AAAAAGAACA GAAACCATTC 
ACACCAGCTT CACCTGTGCA GTCCACACCG AGCAAGCCAT CTGACAAGTC AGGCATGGAT 
GCTGCCTTGG ATGACCTGAT AGACACCTTA GGTGGACATG AAGATACTAA CAGAGATGAT 
CCACCATATA CTGGACCAGT AGTTTTGGAC CCAATGTATT CTACCTACCT TGAGGCACTG 
GGTATAAAAG AAGGGACTAT TCCTCCAGAG TATAGGAAAC TTTTGGAAAA AAATGAAGGG 
ATCACACAAC CTCTTCCAGA CTCTCCTAAA CCTATGGGGA CTGACCAGGC TATAGATGCC 
TTGTCATCTG ATTTCACCTG TAGTTCTCCA ACTGGAAAGC AAAGTGAGAA AGAGAAGTCT 
ACTGGGGAAA TTTTCAAAGC TCAGTCTGCA GGAGTGACCA GAAGTTCTGT TCCCCCCAAG 
GAGAAGAAAA GGAAGGTGGA AGAGGAGGTC ATAAATGATC AAGCACTTCA GGCTCTGTCA 
GATTCACTGG GCACCCGGCA GCCAGATCCT CCGAGCCACG TTAGCCAAGC TGAACAAGTC 
AAAGAGGCAA AAGCAAAAGA AGAAAGGCAG GAGAAGTGTG GTGAAGATGA GGACACAGTC 
CCAGCTGAGT ACAGGTTAAA ACCAGCAAAG GATAAAGATG GAAAACCACT ATTGCCAGAG 
CCTGAAGAAA CATCTAAGAG CCTGAGTGAG TCGGAGCTGA TTGGGGAGCT TTCAGCAGAT 
TTTGACCGAT CTACATATCA AGACAAACCA TCTACGCCAG CTGAAAAGAA ATCCAATGAC 
ACATCCCAAA CTCCTCCGGG GGAGACTGTG CCTCGGGCCT CCATGTGCAG TATACGGTCA 
GCGCCACCCA AACTAGCATC CTTGAAGGGC GTGGTACCAG AAGATGCTGT TGAAACCTTG 
GCTGGAAGCC TGGGGACAAG GGAAGCAGAT CCAGAACATG AAAAAACTGT GGAGGATAAA 
GTCAAGGAGA AAGCTAAAGA AGAAGAGCAT GAAAAACTTG GTGAAAAAGA AGAAACAGTG 
CCTCCTGATT ATCGACTAGA AGAAGTCAAG GATAAGGATG GAAAACCACT CCTGCCCAAA 
GAATCCCAGG AACAACTTGC ACCCTTAAGC GATGACTTCC TTCTTGATGC CTTGTCTCAG 
GACTTCTCCA GTCCGGCAAA CATATCGTCT CTTGAATTTG AAGATGCCAA ACTTTCTGCT 
GCCATTTCTG AAGTAGTTTC TCAGACACCT GCTCCAAGCA CCCATGCAGC AGCTCCACTG 
CCTGGCACTG AGCAGAAAGA CAAAGAACTT GATGATGCCT TGGATGAACT TTCTGACAGT 
CTTGGACAAA GGCCGCCTGA TCCAGATGAG AACAAACCAC TGGATGACAA AGTGAAGGAG 
AAAATCAAAC CAGAGCATAG CGAGAAACTG GGAGAAAGAG ACGACACCAT CCCCCCTGAA 
TACAGGCATC TCTTGGATAA TGATGGGAAG GACAAACCAG AGAAGCCACC GACTAAGAAA 
ACAGAGAAAC CTGATCAGGA CCGGGACCCC ATTGATGCCC TCTCAGAAGA TTTGGATAGC 
TGCCCCTCAA CTACAGAGAC CTCAAAGAAT ACAGCAAAGG GGAAGAGCAA GAAGACTTCA 
AGTTCCAAAG CATCCAAGGA CGGAGAGAAA ACAAAGGACT CTTCCAAGAA GACAGAGGAA 
GTGTCCAAGC CAAAGGCTAA AGAAGATGCA AGACACAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242768.1
CDS	154..2475
Translation	MAFASWWYKTHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSRPHVGEAATATKVTASSAATSKSPSMSTTETKAIPVNKQLEGPDQKRPREQAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_011244466.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244466.2

ORF Insert Sequence:

ATGGCATTCG CAAGTTGGTG GTACAAGACG CATGTTAATG AAAAGAATAT TGGATCATCT 
TCTAAACCAG GAGAAAAGAA AGGATCCGAT GAGAAAAAAG CAGCAAGTTT GGGGAGCAGT 
CAGCCTTCCA GACCACATGT TGGTGAAGCT GCCACAGCCA CCAAGGTGAC AGCTTCCTCT 
GCTGCCACCA GCAAGTCCCC CAGCATGAGT ACCACAGAGA CTAAGGCAAT TCCAGTAAAC 
AAACAGTTGG AAGGACCTGA CCAGAAAAGA CCCAGAGAAC AGGCTGTGAA AACAGAGTCT 
AAGAAACCAC AATCAAGTGA GCAACCTGTG GTTCACGAGA AAAAGTCAAA AGGAGGGCCA 
AAGGAAGGGT CAGAGCCCAA AAATCTTCCG AAGCACACAT CAAGTACAGG AAGCAAGCAT 
GCTCATAAAG AAAAAGCACT TTCCAGATCA AATGAACAGA TGGTGTCAGA GAAACCATCA 
GAATCAAAGA CAAAATTTCA AGATGTTCCT TCAGCTGGTG GAGAGAGTGT GGCTGGAGGT 
GGGACTGTGG CCACTGCCTT GGACAAAGTG GTTGGTAAGA AAAAAGAACA GAAACCATTC 
ACACCAGCTT CACCTGTGCA GTCCACACCG AGCAAGCCAT CTGACAAGTC AGGCATGGAT 
GCTGCCTTGG ATGACCTGAT AGACACCTTA GGTGGACATG AAGATACTAA CAGAGATGAT 
CCACCATATA CTGGACCAGT AGTTTTGGAC CCAATGTATT CTACCTACCT TGAGGCACTG 
GGTATAAAAG AAGGGACTAT TCCTCCAGAG TATAGGAAAC TTTTGGAAAA AAATGAAGGG 
ATCACACAAC CTCTTCCAGA CTCTCCTAAA CCTATGGGGA CTGACCAGGC TATAGATGCC 
TTGTCATCTG ATTTCACCTG TAGTTCTCCA ACTGGAAAGC AAAGTGAGAA AGAGAAGTCT 
ACTGGGGAAA TTTTCAAAGC TCAGTCTGCA GGAGTGACCA GAAGTTCTGT TCCCCCCAAG 
GAGAAGAAAA GGAAGGTGGA AGAGGAGGTC ATAAATGATC AAGCACTTCA GGCTCTGTCA 
GATTCACTGG GCACCCGGCA GCCAGATCCT CCGAGCCACG TTAGCCAAGC TGAACAAGTC 
AAAGAGGCAA AAGCAAAAGA AGAAAGGCAG GAGAAGTGTG GTGAAGATGA GGACACAGTC 
CCAGCTGAGT ACAGGTTAAA ACCAGCAAAG GATAAAGATG GAAAACCACT ATTGCCAGAG 
CCTGAAGAAA CATCTAAGAG CCTGAGTGAG TCGGAGCTGA TTGGGGAGCT TTCAGCAGAT 
TTTGACCGAT CTACATATCA AGACAAACCA TCTACGCCAG CTGAAAAGAA ATCCAATGAC 
ACATCCCAAA CTCCTCCGGG GGAGACTGTG CCTCGGGCCT CCATGTGCAG TATACGGTCA 
GCGCCACCCA AACTAGCATC CTTGAAGGGC GTGGTACCAG AAGATGCTGT TGAAACCTTG 
GCTGGAAGCC TGGGGACAAG GGAAGCAGAT CCAGAACATG AAAAAACTGT GGAGGATAAA 
GTCAAGGAGA AAGCTAAAGA AGAAGAGCAT GAAAAACTTG GTGAAAAAGA AGAAACAGTG 
CCTCCTGATT ATCGACTAGA AGAAGTCAAG GATAAGGATG GAAAACCACT CCTGCCCAAA 
GAATCCCAGG AACAACTTGC ACCCTTAAGC GATGACTTCC TTCTTGATGC CTTGTCTCAG 
GACTTCTCCA GTCCGGCAAA CATATCGTCT CTTGAATTTG AAGATGCCAA ACTTTCTGCT 
GCCATTTCTG AAGTAGTTTC TCAGACACCT GCTCCAAGCA CCCATGCAGC AGCTCCACTG 
CCTGGCACTG AGCAGAAAGA CAAAGAACTT GATGATGCCT TGGATGAACT TTCTGACAGT 
CTTGGACAAA GGCCGCCTGA TCCAGATGAG AACAAACCAC TGGATGACAA AGTGAAGGAG 
AAAATCAAAC CAGAGCATAG CGAGAAACTG GGAGAAAGAG ACGACACCAT CCCCCCTGAA 
TACAGGCATC TCTTGGATAA TGATGGGAAG GACAAACCAG AGAAGCCACC GACTAAGAAA 
ACAGAGAAAC CTGATCAGGA CCGGGACCCC ATTGATGCCC TCTCAGAAGA TTTGGATAGC 
TGCCCCTCAA CTACAGAGAC CTCAAAGAAT ACAGCAAAGG GGAAGAGCAA GAAGACTTCA 
AGTTCCAAAG CATCCAAGGA CGGAGAGAAA ACAAAGGACT CTTCCAAGAA GACAGAGGAA 
GTGTCCAAGC CAAAGGCTAA AGAAGATGCA AGACACAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_011244467.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242769.1 Mus musculus calpastatin (Cast), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu67310
Clone ID Related Accession (Same CDS sequence)	XM_011244467.2 , XM_011244467.3
Accession Version	XM_011244467.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACCA CAGAGACTAA GGCAATTCCA GTAAACAAAC AGTTGGAAGG ACCTGACCAG 
AAAAGACCCA GAGAACAGGC TGTGAAAACA GAGTCTAAGA AACCACAATC AAGTGAGCAA 
CCTGTGGTTC ACGAGAAAAA GTCAAAAGGA GGGCCAAAGG AAGGGTCAGA GCCCAAAAAT 
CTTCCGAAGC ACACATCAAG TACAGGAAGC AAGCATGCTC ATAAAGAAAA AGCACTTTCC 
AGATCAAATG AACAGATGGT GTCAGAGAAA CCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GTTCCTTCAG CTGGTGGAGA GAGTGTGGCT GGAGGTGGGA CTGTGGCCAC TGCCTTGGAC 
AAAGTGGTTG GTAAGAAAAA AGAACAGAAA CCATTCACAC CAGCTTCACC TGTGCAGTCC 
ACACCGAGCA AGCCATCTGA CAAGTCAGGC ATGGATGCTG CCTTGGATGA CCTGATAGAC 
ACCTTAGGTG GACATGAAGA TACTAACAGA GATGATCCAC CATATACTGG ACCAGTAGTT 
TTGGACCCAA TGTATTCTAC CTACCTTGAG GCACTGGGTA TAAAAGAAGG GACTATTCCT 
CCAGAGTATA GGAAACTTTT GGAAAAAAAT GAAGGGATCA CACAACCTCT TCCAGACTCT 
CCTAAACCTA TGGGGACTGA CCAGGCTATA GATGCCTTGT CATCTGATTT CACCTGTAGT 
TCTCCAACTG GAAAGCAAAG TGAGAAAGAG AAGTCTACTG GGGAAATTTT CAAAGCTCAG 
TCTGCAGGAG TGACCAGAAG TTCTGTTCCC CCCAAGGAGA AGAAAAGGAA GGTGGAAGAG 
GAGGTCATAA ATGATCAAGC ACTTCAGGCT CTGTCAGATT CACTGGGCAC CCGGCAGCCA 
GATCCTCCGA GCCACGTTAG CCAAGCTGAA CAAGTCAAAG AGGCAAAAGC AAAAGAAGAA 
AGGCAGGAGA AGTGTGGTGA AGATGAGGAC ACAGTCCCAG CTGAGTACAG GTTAAAACCA 
GCAAAGGATA AAGATGGAAA ACCACTATTG CCAGAGCCTG AAGAAACATC TAAGAGCCTG 
AGTGAGTCGG AGCTGATTGG GGAGCTTTCA GCAGATTTTG ACCGATCTAC ATATCAAGAC 
AAACCATCTA CGCCAGCTGA AAAGAAATCC AATGACACAT CCCAAACTCC TCCGGGGGAG 
ACTGTGCCTC GGGCCTCCAT GTGCAGTATA CGGTCAGCGC CACCCAAACT AGCATCCTTG 
AAGGGCGTGG TACCAGAAGA TGCTGTTGAA ACCTTGGCTG GAAGCCTGGG GACAAGGGAA 
GCAGATCCAG AACATGAAAA AACTGTGGAG GATAAAGTCA AGGAGAAAGC TAAAGAAGAA 
GAGCATGAAA AACTTGGTGA AAAAGAAGAA ACAGTGCCTC CTGATTATCG ACTAGAAGAA 
GTCAAGGATA AGGATGGAAA ACCACTCCTG CCCAAAGAAT CCCAGGAACA ACTTGCACCC 
TTAAGCGATG ACTTCCTTCT TGATGCCTTG TCTCAGGACT TCTCCAGTCC GGCAAACATA 
TCGTCTCTTG AATTTGAAGA TGCCAAACTT TCTGCTGCCA TTTCTGAAGT AGTTTCTCAG 
ACACCTGCTC CAAGCACCCA TGCAGCAGCT CCACTGCCTG GCACTGAGCA GAAAGACAAA 
GAACTTGATG ATGCCTTGGA TGAACTTTCT GACAGTCTTG GACAAAGGCC GCCTGATCCA 
GATGAGAACA AACCACTGGA TGACAAAGTG AAGGAGAAAA TCAAACCAGA GCATAGCGAG 
AAACTGGGAG AAAGAGACGA CACCATCCCC CCTGAATACA GGCATCTCTT GGATAATGAT 
GGGAAGGACA AACCAGAGAA GCCACCGACT AAGAAAACAG AGAAACCTGA TCAGGACCGG 
GACCCCATTG ATGCCCTCTC AGAAGATTTG GATAGCTGCC CCTCAACTAC AGAGACCTCA 
AAGAATACAG CAAAGGGGAA GAGCAAGAAG ACTTCAAGTT CCAAAGCATC CAAGGACGGA 
GAGAAAACAA AGGACTCTTC CAAGAAGACA GAGGAAGTGT CCAAGCCAAA GGCTAAAGAA 
GATGCAAGAC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242769.1
CDS	225..2342
Translation	MSTTETKAIPVNKQLEGPDQKRPREQAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_011244467.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244467.2

ORF Insert Sequence:

ATGAGTACCA CAGAGACTAA GGCAATTCCA GTAAACAAAC AGTTGGAAGG ACCTGACCAG 
AAAAGACCCA GAGAACAGGC TGTGAAAACA GAGTCTAAGA AACCACAATC AAGTGAGCAA 
CCTGTGGTTC ACGAGAAAAA GTCAAAAGGA GGGCCAAAGG AAGGGTCAGA GCCCAAAAAT 
CTTCCGAAGC ACACATCAAG TACAGGAAGC AAGCATGCTC ATAAAGAAAA AGCACTTTCC 
AGATCAAATG AACAGATGGT GTCAGAGAAA CCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GTTCCTTCAG CTGGTGGAGA GAGTGTGGCT GGAGGTGGGA CTGTGGCCAC TGCCTTGGAC 
AAAGTGGTTG GTAAGAAAAA AGAACAGAAA CCATTCACAC CAGCTTCACC TGTGCAGTCC 
ACACCGAGCA AGCCATCTGA CAAGTCAGGC ATGGATGCTG CCTTGGATGA CCTGATAGAC 
ACCTTAGGTG GACATGAAGA TACTAACAGA GATGATCCAC CATATACTGG ACCAGTAGTT 
TTGGACCCAA TGTATTCTAC CTACCTTGAG GCACTGGGTA TAAAAGAAGG GACTATTCCT 
CCAGAGTATA GGAAACTTTT GGAAAAAAAT GAAGGGATCA CACAACCTCT TCCAGACTCT 
CCTAAACCTA TGGGGACTGA CCAGGCTATA GATGCCTTGT CATCTGATTT CACCTGTAGT 
TCTCCAACTG GAAAGCAAAG TGAGAAAGAG AAGTCTACTG GGGAAATTTT CAAAGCTCAG 
TCTGCAGGAG TGACCAGAAG TTCTGTTCCC CCCAAGGAGA AGAAAAGGAA GGTGGAAGAG 
GAGGTCATAA ATGATCAAGC ACTTCAGGCT CTGTCAGATT CACTGGGCAC CCGGCAGCCA 
GATCCTCCGA GCCACGTTAG CCAAGCTGAA CAAGTCAAAG AGGCAAAAGC AAAAGAAGAA 
AGGCAGGAGA AGTGTGGTGA AGATGAGGAC ACAGTCCCAG CTGAGTACAG GTTAAAACCA 
GCAAAGGATA AAGATGGAAA ACCACTATTG CCAGAGCCTG AAGAAACATC TAAGAGCCTG 
AGTGAGTCGG AGCTGATTGG GGAGCTTTCA GCAGATTTTG ACCGATCTAC ATATCAAGAC 
AAACCATCTA CGCCAGCTGA AAAGAAATCC AATGACACAT CCCAAACTCC TCCGGGGGAG 
ACTGTGCCTC GGGCCTCCAT GTGCAGTATA CGGTCAGCGC CACCCAAACT AGCATCCTTG 
AAGGGCGTGG TACCAGAAGA TGCTGTTGAA ACCTTGGCTG GAAGCCTGGG GACAAGGGAA 
GCAGATCCAG AACATGAAAA AACTGTGGAG GATAAAGTCA AGGAGAAAGC TAAAGAAGAA 
GAGCATGAAA AACTTGGTGA AAAAGAAGAA ACAGTGCCTC CTGATTATCG ACTAGAAGAA 
GTCAAGGATA AGGATGGAAA ACCACTCCTG CCCAAAGAAT CCCAGGAACA ACTTGCACCC 
TTAAGCGATG ACTTCCTTCT TGATGCCTTG TCTCAGGACT TCTCCAGTCC GGCAAACATA 
TCGTCTCTTG AATTTGAAGA TGCCAAACTT TCTGCTGCCA TTTCTGAAGT AGTTTCTCAG 
ACACCTGCTC CAAGCACCCA TGCAGCAGCT CCACTGCCTG GCACTGAGCA GAAAGACAAA 
GAACTTGATG ATGCCTTGGA TGAACTTTCT GACAGTCTTG GACAAAGGCC GCCTGATCCA 
GATGAGAACA AACCACTGGA TGACAAAGTG AAGGAGAAAA TCAAACCAGA GCATAGCGAG 
AAACTGGGAG AAAGAGACGA CACCATCCCC CCTGAATACA GGCATCTCTT GGATAATGAT 
GGGAAGGACA AACCAGAGAA GCCACCGACT AAGAAAACAG AGAAACCTGA TCAGGACCGG 
GACCCCATTG ATGCCCTCTC AGAAGATTTG GATAGCTGCC CCTCAACTAC AGAGACCTCA 
AAGAATACAG CAAAGGGGAA GAGCAAGAAG ACTTCAAGTT CCAAAGCATC CAAGGACGGA 
GAGAAAACAA AGGACTCTTC CAAGAAGACA GAGGAAGTGT CCAAGCCAAA GGCTAAAGAA 
GATGCAAGAC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_011244466.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242768.1 Mus musculus calpastatin (Cast), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67309
Clone ID Related Accession (Same CDS sequence)	XM_011244466.2 , XM_011244466.3
Accession Version	XM_011244466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244466.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CAAGTTGGTG GTACAAGACG CATGTTAATG AAAAGAATAT TGGATCATCT 
TCTAAACCAG GAGAAAAGAA AGGATCCGAT GAGAAAAAAG CAGCAAGTTT GGGGAGCAGT 
CAGCCTTCCA GACCACATGT TGGTGAAGCT GCCACAGCCA CCAAGGTGAC AGCTTCCTCT 
GCTGCCACCA GCAAGTCCCC CAGCATGAGT ACCACAGAGA CTAAGGCAAT TCCAGTAAAC 
AAACAGTTGG AAGGACCTGA CCAGAAAAGA CCCAGAGAAC AGGCTGTGAA AACAGAGTCT 
AAGAAACCAC AATCAAGTGA GCAACCTGTG GTTCACGAGA AAAAGTCAAA AGGAGGGCCA 
AAGGAAGGGT CAGAGCCCAA AAATCTTCCG AAGCACACAT CAAGTACAGG AAGCAAGCAT 
GCTCATAAAG AAAAAGCACT TTCCAGATCA AATGAACAGA TGGTGTCAGA GAAACCATCA 
GAATCAAAGA CAAAATTTCA AGATGTTCCT TCAGCTGGTG GAGAGAGTGT GGCTGGAGGT 
GGGACTGTGG CCACTGCCTT GGACAAAGTG GTTGGTAAGA AAAAAGAACA GAAACCATTC 
ACACCAGCTT CACCTGTGCA GTCCACACCG AGCAAGCCAT CTGACAAGTC AGGCATGGAT 
GCTGCCTTGG ATGACCTGAT AGACACCTTA GGTGGACATG AAGATACTAA CAGAGATGAT 
CCACCATATA CTGGACCAGT AGTTTTGGAC CCAATGTATT CTACCTACCT TGAGGCACTG 
GGTATAAAAG AAGGGACTAT TCCTCCAGAG TATAGGAAAC TTTTGGAAAA AAATGAAGGG 
ATCACACAAC CTCTTCCAGA CTCTCCTAAA CCTATGGGGA CTGACCAGGC TATAGATGCC 
TTGTCATCTG ATTTCACCTG TAGTTCTCCA ACTGGAAAGC AAAGTGAGAA AGAGAAGTCT 
ACTGGGGAAA TTTTCAAAGC TCAGTCTGCA GGAGTGACCA GAAGTTCTGT TCCCCCCAAG 
GAGAAGAAAA GGAAGGTGGA AGAGGAGGTC ATAAATGATC AAGCACTTCA GGCTCTGTCA 
GATTCACTGG GCACCCGGCA GCCAGATCCT CCGAGCCACG TTAGCCAAGC TGAACAAGTC 
AAAGAGGCAA AAGCAAAAGA AGAAAGGCAG GAGAAGTGTG GTGAAGATGA GGACACAGTC 
CCAGCTGAGT ACAGGTTAAA ACCAGCAAAG GATAAAGATG GAAAACCACT ATTGCCAGAG 
CCTGAAGAAA CATCTAAGAG CCTGAGTGAG TCGGAGCTGA TTGGGGAGCT TTCAGCAGAT 
TTTGACCGAT CTACATATCA AGACAAACCA TCTACGCCAG CTGAAAAGAA ATCCAATGAC 
ACATCCCAAA CTCCTCCGGG GGAGACTGTG CCTCGGGCCT CCATGTGCAG TATACGGTCA 
GCGCCACCCA AACTAGCATC CTTGAAGGGC GTGGTACCAG AAGATGCTGT TGAAACCTTG 
GCTGGAAGCC TGGGGACAAG GGAAGCAGAT CCAGAACATG AAAAAACTGT GGAGGATAAA 
GTCAAGGAGA AAGCTAAAGA AGAAGAGCAT GAAAAACTTG GTGAAAAAGA AGAAACAGTG 
CCTCCTGATT ATCGACTAGA AGAAGTCAAG GATAAGGATG GAAAACCACT CCTGCCCAAA 
GAATCCCAGG AACAACTTGC ACCCTTAAGC GATGACTTCC TTCTTGATGC CTTGTCTCAG 
GACTTCTCCA GTCCGGCAAA CATATCGTCT CTTGAATTTG AAGATGCCAA ACTTTCTGCT 
GCCATTTCTG AAGTAGTTTC TCAGACACCT GCTCCAAGCA CCCATGCAGC AGCTCCACTG 
CCTGGCACTG AGCAGAAAGA CAAAGAACTT GATGATGCCT TGGATGAACT TTCTGACAGT 
CTTGGACAAA GGCCGCCTGA TCCAGATGAG AACAAACCAC TGGATGACAA AGTGAAGGAG 
AAAATCAAAC CAGAGCATAG CGAGAAACTG GGAGAAAGAG ACGACACCAT CCCCCCTGAA 
TACAGGCATC TCTTGGATAA TGATGGGAAG GACAAACCAG AGAAGCCACC GACTAAGAAA 
ACAGAGAAAC CTGATCAGGA CCGGGACCCC ATTGATGCCC TCTCAGAAGA TTTGGATAGC 
TGCCCCTCAA CTACAGAGAC CTCAAAGAAT ACAGCAAAGG GGAAGAGCAA GAAGACTTCA 
AGTTCCAAAG CATCCAAGGA CGGAGAGAAA ACAAAGGACT CTTCCAAGAA GACAGAGGAA 
GTGTCCAAGC CAAAGGCTAA AGAAGATGCA AGACACAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242768.1
CDS	144..2465
Translation	MAFASWWYKTHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSRPHVGEAATATKVTASSAATSKSPSMSTTETKAIPVNKQLEGPDQKRPREQAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_011244466.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244466.3

ORF Insert Sequence:

ATGGCATTCG CAAGTTGGTG GTACAAGACG CATGTTAATG AAAAGAATAT TGGATCATCT 
TCTAAACCAG GAGAAAAGAA AGGATCCGAT GAGAAAAAAG CAGCAAGTTT GGGGAGCAGT 
CAGCCTTCCA GACCACATGT TGGTGAAGCT GCCACAGCCA CCAAGGTGAC AGCTTCCTCT 
GCTGCCACCA GCAAGTCCCC CAGCATGAGT ACCACAGAGA CTAAGGCAAT TCCAGTAAAC 
AAACAGTTGG AAGGACCTGA CCAGAAAAGA CCCAGAGAAC AGGCTGTGAA AACAGAGTCT 
AAGAAACCAC AATCAAGTGA GCAACCTGTG GTTCACGAGA AAAAGTCAAA AGGAGGGCCA 
AAGGAAGGGT CAGAGCCCAA AAATCTTCCG AAGCACACAT CAAGTACAGG AAGCAAGCAT 
GCTCATAAAG AAAAAGCACT TTCCAGATCA AATGAACAGA TGGTGTCAGA GAAACCATCA 
GAATCAAAGA CAAAATTTCA AGATGTTCCT TCAGCTGGTG GAGAGAGTGT GGCTGGAGGT 
GGGACTGTGG CCACTGCCTT GGACAAAGTG GTTGGTAAGA AAAAAGAACA GAAACCATTC 
ACACCAGCTT CACCTGTGCA GTCCACACCG AGCAAGCCAT CTGACAAGTC AGGCATGGAT 
GCTGCCTTGG ATGACCTGAT AGACACCTTA GGTGGACATG AAGATACTAA CAGAGATGAT 
CCACCATATA CTGGACCAGT AGTTTTGGAC CCAATGTATT CTACCTACCT TGAGGCACTG 
GGTATAAAAG AAGGGACTAT TCCTCCAGAG TATAGGAAAC TTTTGGAAAA AAATGAAGGG 
ATCACACAAC CTCTTCCAGA CTCTCCTAAA CCTATGGGGA CTGACCAGGC TATAGATGCC 
TTGTCATCTG ATTTCACCTG TAGTTCTCCA ACTGGAAAGC AAAGTGAGAA AGAGAAGTCT 
ACTGGGGAAA TTTTCAAAGC TCAGTCTGCA GGAGTGACCA GAAGTTCTGT TCCCCCCAAG 
GAGAAGAAAA GGAAGGTGGA AGAGGAGGTC ATAAATGATC AAGCACTTCA GGCTCTGTCA 
GATTCACTGG GCACCCGGCA GCCAGATCCT CCGAGCCACG TTAGCCAAGC TGAACAAGTC 
AAAGAGGCAA AAGCAAAAGA AGAAAGGCAG GAGAAGTGTG GTGAAGATGA GGACACAGTC 
CCAGCTGAGT ACAGGTTAAA ACCAGCAAAG GATAAAGATG GAAAACCACT ATTGCCAGAG 
CCTGAAGAAA CATCTAAGAG CCTGAGTGAG TCGGAGCTGA TTGGGGAGCT TTCAGCAGAT 
TTTGACCGAT CTACATATCA AGACAAACCA TCTACGCCAG CTGAAAAGAA ATCCAATGAC 
ACATCCCAAA CTCCTCCGGG GGAGACTGTG CCTCGGGCCT CCATGTGCAG TATACGGTCA 
GCGCCACCCA AACTAGCATC CTTGAAGGGC GTGGTACCAG AAGATGCTGT TGAAACCTTG 
GCTGGAAGCC TGGGGACAAG GGAAGCAGAT CCAGAACATG AAAAAACTGT GGAGGATAAA 
GTCAAGGAGA AAGCTAAAGA AGAAGAGCAT GAAAAACTTG GTGAAAAAGA AGAAACAGTG 
CCTCCTGATT ATCGACTAGA AGAAGTCAAG GATAAGGATG GAAAACCACT CCTGCCCAAA 
GAATCCCAGG AACAACTTGC ACCCTTAAGC GATGACTTCC TTCTTGATGC CTTGTCTCAG 
GACTTCTCCA GTCCGGCAAA CATATCGTCT CTTGAATTTG AAGATGCCAA ACTTTCTGCT 
GCCATTTCTG AAGTAGTTTC TCAGACACCT GCTCCAAGCA CCCATGCAGC AGCTCCACTG 
CCTGGCACTG AGCAGAAAGA CAAAGAACTT GATGATGCCT TGGATGAACT TTCTGACAGT 
CTTGGACAAA GGCCGCCTGA TCCAGATGAG AACAAACCAC TGGATGACAA AGTGAAGGAG 
AAAATCAAAC CAGAGCATAG CGAGAAACTG GGAGAAAGAG ACGACACCAT CCCCCCTGAA 
TACAGGCATC TCTTGGATAA TGATGGGAAG GACAAACCAG AGAAGCCACC GACTAAGAAA 
ACAGAGAAAC CTGATCAGGA CCGGGACCCC ATTGATGCCC TCTCAGAAGA TTTGGATAGC 
TGCCCCTCAA CTACAGAGAC CTCAAAGAAT ACAGCAAAGG GGAAGAGCAA GAAGACTTCA 
AGTTCCAAAG CATCCAAGGA CGGAGAGAAA ACAAAGGACT CTTCCAAGAA GACAGAGGAA 
GTGTCCAAGC CAAAGGCTAA AGAAGATGCA AGACACAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_011244467.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242769.1 Mus musculus calpastatin (Cast), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu67310
Clone ID Related Accession (Same CDS sequence)	XM_011244467.2 , XM_011244467.3
Accession Version	XM_011244467.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244467.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACCA CAGAGACTAA GGCAATTCCA GTAAACAAAC AGTTGGAAGG ACCTGACCAG 
AAAAGACCCA GAGAACAGGC TGTGAAAACA GAGTCTAAGA AACCACAATC AAGTGAGCAA 
CCTGTGGTTC ACGAGAAAAA GTCAAAAGGA GGGCCAAAGG AAGGGTCAGA GCCCAAAAAT 
CTTCCGAAGC ACACATCAAG TACAGGAAGC AAGCATGCTC ATAAAGAAAA AGCACTTTCC 
AGATCAAATG AACAGATGGT GTCAGAGAAA CCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GTTCCTTCAG CTGGTGGAGA GAGTGTGGCT GGAGGTGGGA CTGTGGCCAC TGCCTTGGAC 
AAAGTGGTTG GTAAGAAAAA AGAACAGAAA CCATTCACAC CAGCTTCACC TGTGCAGTCC 
ACACCGAGCA AGCCATCTGA CAAGTCAGGC ATGGATGCTG CCTTGGATGA CCTGATAGAC 
ACCTTAGGTG GACATGAAGA TACTAACAGA GATGATCCAC CATATACTGG ACCAGTAGTT 
TTGGACCCAA TGTATTCTAC CTACCTTGAG GCACTGGGTA TAAAAGAAGG GACTATTCCT 
CCAGAGTATA GGAAACTTTT GGAAAAAAAT GAAGGGATCA CACAACCTCT TCCAGACTCT 
CCTAAACCTA TGGGGACTGA CCAGGCTATA GATGCCTTGT CATCTGATTT CACCTGTAGT 
TCTCCAACTG GAAAGCAAAG TGAGAAAGAG AAGTCTACTG GGGAAATTTT CAAAGCTCAG 
TCTGCAGGAG TGACCAGAAG TTCTGTTCCC CCCAAGGAGA AGAAAAGGAA GGTGGAAGAG 
GAGGTCATAA ATGATCAAGC ACTTCAGGCT CTGTCAGATT CACTGGGCAC CCGGCAGCCA 
GATCCTCCGA GCCACGTTAG CCAAGCTGAA CAAGTCAAAG AGGCAAAAGC AAAAGAAGAA 
AGGCAGGAGA AGTGTGGTGA AGATGAGGAC ACAGTCCCAG CTGAGTACAG GTTAAAACCA 
GCAAAGGATA AAGATGGAAA ACCACTATTG CCAGAGCCTG AAGAAACATC TAAGAGCCTG 
AGTGAGTCGG AGCTGATTGG GGAGCTTTCA GCAGATTTTG ACCGATCTAC ATATCAAGAC 
AAACCATCTA CGCCAGCTGA AAAGAAATCC AATGACACAT CCCAAACTCC TCCGGGGGAG 
ACTGTGCCTC GGGCCTCCAT GTGCAGTATA CGGTCAGCGC CACCCAAACT AGCATCCTTG 
AAGGGCGTGG TACCAGAAGA TGCTGTTGAA ACCTTGGCTG GAAGCCTGGG GACAAGGGAA 
GCAGATCCAG AACATGAAAA AACTGTGGAG GATAAAGTCA AGGAGAAAGC TAAAGAAGAA 
GAGCATGAAA AACTTGGTGA AAAAGAAGAA ACAGTGCCTC CTGATTATCG ACTAGAAGAA 
GTCAAGGATA AGGATGGAAA ACCACTCCTG CCCAAAGAAT CCCAGGAACA ACTTGCACCC 
TTAAGCGATG ACTTCCTTCT TGATGCCTTG TCTCAGGACT TCTCCAGTCC GGCAAACATA 
TCGTCTCTTG AATTTGAAGA TGCCAAACTT TCTGCTGCCA TTTCTGAAGT AGTTTCTCAG 
ACACCTGCTC CAAGCACCCA TGCAGCAGCT CCACTGCCTG GCACTGAGCA GAAAGACAAA 
GAACTTGATG ATGCCTTGGA TGAACTTTCT GACAGTCTTG GACAAAGGCC GCCTGATCCA 
GATGAGAACA AACCACTGGA TGACAAAGTG AAGGAGAAAA TCAAACCAGA GCATAGCGAG 
AAACTGGGAG AAAGAGACGA CACCATCCCC CCTGAATACA GGCATCTCTT GGATAATGAT 
GGGAAGGACA AACCAGAGAA GCCACCGACT AAGAAAACAG AGAAACCTGA TCAGGACCGG 
GACCCCATTG ATGCCCTCTC AGAAGATTTG GATAGCTGCC CCTCAACTAC AGAGACCTCA 
AAGAATACAG CAAAGGGGAA GAGCAAGAAG ACTTCAAGTT CCAAAGCATC CAAGGACGGA 
GAGAAAACAA AGGACTCTTC CAAGAAGACA GAGGAAGTGT CCAAGCCAAA GGCTAAAGAA 
GATGCAAGAC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242769.1
CDS	224..2341
Translation	MSTTETKAIPVNKQLEGPDQKRPREQAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_011244467.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244467.3

ORF Insert Sequence:

ATGAGTACCA CAGAGACTAA GGCAATTCCA GTAAACAAAC AGTTGGAAGG ACCTGACCAG 
AAAAGACCCA GAGAACAGGC TGTGAAAACA GAGTCTAAGA AACCACAATC AAGTGAGCAA 
CCTGTGGTTC ACGAGAAAAA GTCAAAAGGA GGGCCAAAGG AAGGGTCAGA GCCCAAAAAT 
CTTCCGAAGC ACACATCAAG TACAGGAAGC AAGCATGCTC ATAAAGAAAA AGCACTTTCC 
AGATCAAATG AACAGATGGT GTCAGAGAAA CCATCAGAAT CAAAGACAAA ATTTCAAGAT 
GTTCCTTCAG CTGGTGGAGA GAGTGTGGCT GGAGGTGGGA CTGTGGCCAC TGCCTTGGAC 
AAAGTGGTTG GTAAGAAAAA AGAACAGAAA CCATTCACAC CAGCTTCACC TGTGCAGTCC 
ACACCGAGCA AGCCATCTGA CAAGTCAGGC ATGGATGCTG CCTTGGATGA CCTGATAGAC 
ACCTTAGGTG GACATGAAGA TACTAACAGA GATGATCCAC CATATACTGG ACCAGTAGTT 
TTGGACCCAA TGTATTCTAC CTACCTTGAG GCACTGGGTA TAAAAGAAGG GACTATTCCT 
CCAGAGTATA GGAAACTTTT GGAAAAAAAT GAAGGGATCA CACAACCTCT TCCAGACTCT 
CCTAAACCTA TGGGGACTGA CCAGGCTATA GATGCCTTGT CATCTGATTT CACCTGTAGT 
TCTCCAACTG GAAAGCAAAG TGAGAAAGAG AAGTCTACTG GGGAAATTTT CAAAGCTCAG 
TCTGCAGGAG TGACCAGAAG TTCTGTTCCC CCCAAGGAGA AGAAAAGGAA GGTGGAAGAG 
GAGGTCATAA ATGATCAAGC ACTTCAGGCT CTGTCAGATT CACTGGGCAC CCGGCAGCCA 
GATCCTCCGA GCCACGTTAG CCAAGCTGAA CAAGTCAAAG AGGCAAAAGC AAAAGAAGAA 
AGGCAGGAGA AGTGTGGTGA AGATGAGGAC ACAGTCCCAG CTGAGTACAG GTTAAAACCA 
GCAAAGGATA AAGATGGAAA ACCACTATTG CCAGAGCCTG AAGAAACATC TAAGAGCCTG 
AGTGAGTCGG AGCTGATTGG GGAGCTTTCA GCAGATTTTG ACCGATCTAC ATATCAAGAC 
AAACCATCTA CGCCAGCTGA AAAGAAATCC AATGACACAT CCCAAACTCC TCCGGGGGAG 
ACTGTGCCTC GGGCCTCCAT GTGCAGTATA CGGTCAGCGC CACCCAAACT AGCATCCTTG 
AAGGGCGTGG TACCAGAAGA TGCTGTTGAA ACCTTGGCTG GAAGCCTGGG GACAAGGGAA 
GCAGATCCAG AACATGAAAA AACTGTGGAG GATAAAGTCA AGGAGAAAGC TAAAGAAGAA 
GAGCATGAAA AACTTGGTGA AAAAGAAGAA ACAGTGCCTC CTGATTATCG ACTAGAAGAA 
GTCAAGGATA AGGATGGAAA ACCACTCCTG CCCAAAGAAT CCCAGGAACA ACTTGCACCC 
TTAAGCGATG ACTTCCTTCT TGATGCCTTG TCTCAGGACT TCTCCAGTCC GGCAAACATA 
TCGTCTCTTG AATTTGAAGA TGCCAAACTT TCTGCTGCCA TTTCTGAAGT AGTTTCTCAG 
ACACCTGCTC CAAGCACCCA TGCAGCAGCT CCACTGCCTG GCACTGAGCA GAAAGACAAA 
GAACTTGATG ATGCCTTGGA TGAACTTTCT GACAGTCTTG GACAAAGGCC GCCTGATCCA 
GATGAGAACA AACCACTGGA TGACAAAGTG AAGGAGAAAA TCAAACCAGA GCATAGCGAG 
AAACTGGGAG AAAGAGACGA CACCATCCCC CCTGAATACA GGCATCTCTT GGATAATGAT 
GGGAAGGACA AACCAGAGAA GCCACCGACT AAGAAAACAG AGAAACCTGA TCAGGACCGG 
GACCCCATTG ATGCCCTCTC AGAAGATTTG GATAGCTGCC CCTCAACTAC AGAGACCTCA 
AAGAATACAG CAAAGGGGAA GAGCAAGAAG ACTTCAAGTT CCAAAGCATC CAAGGACGGA 
GAGAAAACAA AGGACTCTTC CAAGAAGACA GAGGAAGTGT CCAAGCCAAA GGCTAAAGAA 
GATGCAAGAC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cast ( XM_017315374.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170863.1 Mus musculus calpastatin (Cast), transcript variant X5, mRNA.

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CloneID	OMu78421
Clone ID Related Accession (Same CDS sequence)	XM_017315374.1
Accession Version	XM_017315374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpastatin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACCA CAGAGACTAA GGCTGTGAAA ACAGAGTCTA AGAAACCACA ATCAAGTGAG 
CAACCTGTGG TTCACGAGAA AAAGTCAAAA GGAGGGCCAA AGGAAGGGTC AGAGCCCAAA 
AATCTTCCGA AGCACACATC AAGTACAGGA AGCAAGCATG CTCATAAAGA AAAAGCACTT 
TCCAGATCAA ATGAACAGAT GGTGTCAGAG AAACCATCAG AATCAAAGAC AAAATTTCAA 
GATGTTCCTT CAGCTGGTGG AGAGAGTGTG GCTGGAGGTG GGACTGTGGC CACTGCCTTG 
GACAAAGTGG TTGGTAAGAA AAAAGAACAG AAACCATTCA CACCAGCTTC ACCTGTGCAG 
TCCACACCGA GCAAGCCATC TGACAAGTCA GGCATGGATG CTGCCTTGGA TGACCTGATA 
GACACCTTAG GTGGACATGA AGATACTAAC AGAGATGATC CACCATATAC TGGACCAGTA 
GTTTTGGACC CAATGTATTC TACCTACCTT GAGGCACTGG GTATAAAAGA AGGGACTATT 
CCTCCAGAGT ATAGGAAACT TTTGGAAAAA AATGAAGGGA TCACACAACC TCTTCCAGAC 
TCTCCTAAAC CTATGGGGAC TGACCAGGCT ATAGATGCCT TGTCATCTGA TTTCACCTGT 
AGTTCTCCAA CTGGAAAGCA AAGTGAGAAA GAGAAGTCTA CTGGGGAAAT TTTCAAAGCT 
CAGTCTGCAG GAGTGACCAG AAGTTCTGTT CCCCCCAAGG AGAAGAAAAG GAAGGTGGAA 
GAGGAGGTCA TAAATGATCA AGCACTTCAG GCTCTGTCAG ATTCACTGGG CACCCGGCAG 
CCAGATCCTC CGAGCCACGT TAGCCAAGCT GAACAAGTCA AAGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GAAAGACAAA 
GAACTTGATG ATGCCTTGGA TGAACTTTCT GACAGTCTTG GACAAAGGCC GCCTGATCCA 
GATGAGAACA AACCACTGGA TGACAAAGTG AAGGAGAAAA TCAAACCAGA GCATAGCGAG 
AAACTGGGAG AAAGAGACGA CACCATCCCC CCTGAATACA GGCATCTCTT GGATAATGAT 
GGGAAGGACA AACCAGAGAA GCCACCGACT AAGAAAACAG AGAAACCTGA TCAGGACCGG 
GACCCCATTG ATGCCCTCTC AGAAGATTTG GATAGCTGCC CCTCAACTAC AGAGACCTCA 
AAGAATACAG CAAAGGGGAA GAGCAAGAAG ACTTCAAGTT CCAAAGCATC CAAGGACGGA 
GAGAAAACAA AGGACTCTTC CAAGAAGACA GAGGAAGTGT CCAAGCCAAA GGCTAAAGAA 
GATGCAAGAC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170863.1
CDS	226..2283
Translation	MSTTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEKDKELDDALDELSDSLGQRPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

Target ORF information:

RefSeq Version	XM_017315374.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpastatin (Cast), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315374.1

ORF Insert Sequence:

ATGAGTACCA CAGAGACTAA GGCTGTGAAA ACAGAGTCTA AGAAACCACA ATCAAGTGAG 
CAACCTGTGG TTCACGAGAA AAAGTCAAAA GGAGGGCCAA AGGAAGGGTC AGAGCCCAAA 
AATCTTCCGA AGCACACATC AAGTACAGGA AGCAAGCATG CTCATAAAGA AAAAGCACTT 
TCCAGATCAA ATGAACAGAT GGTGTCAGAG AAACCATCAG AATCAAAGAC AAAATTTCAA 
GATGTTCCTT CAGCTGGTGG AGAGAGTGTG GCTGGAGGTG GGACTGTGGC CACTGCCTTG 
GACAAAGTGG TTGGTAAGAA AAAAGAACAG AAACCATTCA CACCAGCTTC ACCTGTGCAG 
TCCACACCGA GCAAGCCATC TGACAAGTCA GGCATGGATG CTGCCTTGGA TGACCTGATA 
GACACCTTAG GTGGACATGA AGATACTAAC AGAGATGATC CACCATATAC TGGACCAGTA 
GTTTTGGACC CAATGTATTC TACCTACCTT GAGGCACTGG GTATAAAAGA AGGGACTATT 
CCTCCAGAGT ATAGGAAACT TTTGGAAAAA AATGAAGGGA TCACACAACC TCTTCCAGAC 
TCTCCTAAAC CTATGGGGAC TGACCAGGCT ATAGATGCCT TGTCATCTGA TTTCACCTGT 
AGTTCTCCAA CTGGAAAGCA AAGTGAGAAA GAGAAGTCTA CTGGGGAAAT TTTCAAAGCT 
CAGTCTGCAG GAGTGACCAG AAGTTCTGTT CCCCCCAAGG AGAAGAAAAG GAAGGTGGAA 
GAGGAGGTCA TAAATGATCA AGCACTTCAG GCTCTGTCAG ATTCACTGGG CACCCGGCAG 
CCAGATCCTC CGAGCCACGT TAGCCAAGCT GAACAAGTCA AAGAGGCAAA AGCAAAAGAA 
GAAAGGCAGG AGAAGTGTGG TGAAGATGAG GACACAGTCC CAGCTGAGTA CAGGTTAAAA 
CCAGCAAAGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAC ATCTAAGAGC 
CTGAGTGAGT CGGAGCTGAT TGGGGAGCTT TCAGCAGATT TTGACCGATC TACATATCAA 
GACAAACCAT CTACGCCAGC TGAAAAGAAA TCCAATGACA CATCCCAAAC TCCTCCGGGG 
GAGACTGTGC CTCGGGCCTC CATGTGCAGT ATACGGTCAG CGCCACCCAA ACTAGCATCC 
TTGAAGGGCG TGGTACCAGA AGATGCTGTT GAAACCTTGG CTGGAAGCCT GGGGACAAGG 
GAAGCAGATC CAGAACATGA AAAAACTGTG GAGGATAAAG TCAAGGAGAA AGCTAAAGAA 
GAAGAGCATG AAAAACTTGG TGAAAAAGAA GAAACAGTGC CTCCTGATTA TCGACTAGAA 
GAAGTCAAGG ATAAGGATGG AAAACCACTC CTGCCCAAAG AATCCCAGGA ACAACTTGCA 
CCCTTAAGCG ATGACTTCCT TCTTGATGCC TTGTCTCAGG ACTTCTCCAG TCCGGCAAAC 
ATATCGTCTC TTGAATTTGA AGATGCCAAA CTTTCTGCTG CCATTTCTGA AGTAGTTTCT 
CAGACACCTG CTCCAAGCAC CCATGCAGCA GCTCCACTGC CTGGCACTGA GAAAGACAAA 
GAACTTGATG ATGCCTTGGA TGAACTTTCT GACAGTCTTG GACAAAGGCC GCCTGATCCA 
GATGAGAACA AACCACTGGA TGACAAAGTG AAGGAGAAAA TCAAACCAGA GCATAGCGAG 
AAACTGGGAG AAAGAGACGA CACCATCCCC CCTGAATACA GGCATCTCTT GGATAATGAT 
GGGAAGGACA AACCAGAGAA GCCACCGACT AAGAAAACAG AGAAACCTGA TCAGGACCGG 
GACCCCATTG ATGCCCTCTC AGAAGATTTG GATAGCTGCC CCTCAACTAC AGAGACCTCA 
AAGAATACAG CAAAGGGGAA GAGCAAGAAG ACTTCAAGTT CCAAAGCATC CAAGGACGGA 
GAGAAAACAA AGGACTCTTC CAAGAAGACA GAGGAAGTGT CCAAGCCAAA GGCTAAAGAA 
GATGCAAGAC ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Cast
Entrez Gene ID	12380
Full Name	calpastatin
General protein information	Preferred Names calpastatin Names calpastatin calpain inhibitor calpastatin type III
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 C1
Summary	This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. [provided by RefSeq, Jul 2014].

Homo sapiens (human)	CAST	NP_001035905.1
	CAST	XP_001091928.2
Mus musculus (house mouse)	Cast	NP_033947.1
Rattus norvegicus (Norway rat)	Cast	NP_445747.2
Gallus gallus (chicken)	CAST	NP_001131122.1
Pan troglodytes (chimpanzee)	CAST	XP_518168.4
Canis lupus familiaris (dog)	CAST	XP_005618134.1
Bos taurus (cattle)	CAST	NP_001025489.1
Xenopus tropicalis (tropical clawed frog)	cast	NP_001120309.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Cast cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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