CAST cDNA ORF clone, Homo sapiens(human)

The following CAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16872 NM_001284212.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 13, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu20713 NM_001042440.5
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu21528 NM_173060.4
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu39740 NM_001042444.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu39744 XM_006714715.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X47, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu21399 NM_001284213.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu39741 NM_001042446.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu39742 XM_006714713.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X45, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu39743 XM_006714714.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X46, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu56646 XM_011543658.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu21220 NM_001330631.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu21220 NM_001190442.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39736 NM_001330628.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39737 NM_001042443.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39738 NM_001330633.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39739 NM_001042445.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu21220 XM_006714708.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39729 XM_006714699.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39732 XM_006714702.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu39734 XM_006714704.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu56645 XM_011543657.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu102190 NM_001330630.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu102283 NM_001330632.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu102294 NM_001330634.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu102380 NM_001330629.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu39731 NM_001042442.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu39734 NM_001375317.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96116 XM_017009920.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96117 XM_017009921.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96121 XM_017009925.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96122 XM_017009926.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96123 XM_017009927.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96124 XM_017009928.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96125 XM_017009931.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu102305 NM_001330626.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39726 NM_001750.7
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39730 NM_001042441.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39735 NM_001330627.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant 18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39726 XM_006714696.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39727 XM_006714697.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39728 XM_006714698.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39730 XM_017009913.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39731 XM_006714701.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39733 XM_006714703.3
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu56642 XM_011543654.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu56643 XM_011543655.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu56644 XM_011543656.2
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96112 XM_017009911.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96112 XM_017009912.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96113 XM_017009914.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96114 XM_017009916.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96115 XM_017009919.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96118 XM_017009922.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96119 XM_017009923.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu96120 XM_017009924.1
Latest version!
Homo sapiens calpastatin (CAST), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
View Old Accession Versions >>
OHu102380 NM_001330629.1 Homo sapiens calpastatin (CAST), transcript variant 20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu21528 NM_173060.3 Homo sapiens calpastatin (CAST), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu39735 NM_001330627.1 Homo sapiens calpastatin (CAST), transcript variant 18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu20713 NM_001042440.3 Homo sapiens calpastatin (CAST), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu39736 NM_001330628.1 Homo sapiens calpastatin (CAST), transcript variant 19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu20713 NM_001042440.4 Homo sapiens calpastatin (CAST), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu39726 NM_001750.6 Homo sapiens calpastatin (CAST), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu102305 NM_001330626.1 Homo sapiens calpastatin (CAST), transcript variant 17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39730 NM_001042441.2 Homo sapiens calpastatin (CAST), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu39731 NM_001042442.2 Homo sapiens calpastatin (CAST), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu16872 NM_001284212.1 Homo sapiens calpastatin (CAST), transcript variant 13, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16872
    Clone ID Related Accession (Same CDS sequence) NM_001284212.1 , NM_001284212.3
    Accession Version NM_001284212.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-12
    Organism Homo sapiens(Human)
    Product calpastatin isoform n
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC
    TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT
    GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA
    GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG
    GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG
    CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA
    GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA
    AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG
    CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT
    AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT
    CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG
    CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG
    GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG
    GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT
    AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA
    CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT
    CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA
    GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG
    AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG
    CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA
    CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG
    GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG
    AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC
    TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC
    TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT
    TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001271141.1
    Translation

    Target ORF information:

    RefSeq Version NM_001284212.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001284212.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC
    TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT
    GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA
    GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG
    GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG
    CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA
    GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA
    AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG
    CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT
    AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT
    CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG
    CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG
    GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG
    GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT
    AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA
    CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT
    CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA
    GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG
    AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG
    CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA
    CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG
    GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG
    AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC
    TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC
    TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT
    TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16872
    Clone ID Related Accession (Same CDS sequence) NM_001284212.1 , NM_001284212.3
    Accession Version NM_001284212.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform n
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, BX648397.1, U31345.1 and AC008906.5. On Aug 30, 2016 this sequence version replaced NM_001284212.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.23360.1, SRR1803611.111838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC
    TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT
    GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA
    GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG
    GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG
    CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA
    GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA
    AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG
    CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT
    AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT
    CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG
    CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG
    GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG
    GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT
    AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA
    CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT
    CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA
    GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG
    AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG
    CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA
    CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG
    GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG
    AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC
    TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC
    TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT
    TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001271141.1
    CDS296..2296
    Translation

    Target ORF information:

    RefSeq Version NM_001284212.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001284212.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC
    TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT
    GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA
    GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG
    GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG
    CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA
    GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA
    AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG
    CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT
    AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT
    CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG
    CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG
    GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG
    GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT
    AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA
    CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT
    CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA
    GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG
    AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG
    CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA
    CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG
    GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG
    AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC
    TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC
    TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT
    TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20713
    Clone ID Related Accession (Same CDS sequence) NM_001042440.3 , NM_001042440.4 , NM_001042440.5
    Accession Version NM_001042440.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-12
    Organism Homo sapiens(Human)
    Product calpastatin isoform f
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035905.1
    Translation

    Target ORF information:

    RefSeq Version NM_001042440.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042440.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20713
    Clone ID Related Accession (Same CDS sequence) NM_001042440.3 , NM_001042440.4 , NM_001042440.5
    Accession Version NM_001042440.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product calpastatin isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced NM_001042440.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.47151.1, SRR1803613.226150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035905.1
    CDS267..2519
    Translation

    Target ORF information:

    RefSeq Version NM_001042440.4
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042440.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20713
    Clone ID Related Accession (Same CDS sequence) NM_001042440.3 , NM_001042440.4 , NM_001042440.5
    Accession Version NM_001042440.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001042440.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.226150.1, SRR1803613.6171.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035905.1
    CDS48..2300
    Translation

    Target ORF information:

    RefSeq Version NM_001042440.5
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042440.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21528
    Clone ID Related Accession (Same CDS sequence) NM_173060.3 , NM_173060.4
    Accession Version NM_173060.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-12
    Organism Homo sapiens(Human)
    Product calpastatin isoform b
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT
    TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG
    TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT
    CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG
    AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA
    GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA
    GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA
    CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT
    CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA
    GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT
    GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG
    GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG
    AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC
    AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA
    TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG
    CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA
    CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG
    GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGCAG
    AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG
    CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA
    CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG
    GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG
    AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC
    TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC
    TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT
    TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_775083.1
    Translation

    Target ORF information:

    RefSeq Version NM_173060.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_173060.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT
    TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG
    TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT
    CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG
    AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA
    GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA
    GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA
    CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT
    CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA
    GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT
    GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG
    GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG
    AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC
    AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA
    TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG
    CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA
    CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG
    GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGCAG
    AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG
    CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA
    CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG
    GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG
    AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC
    TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC
    TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT
    TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21528
    Clone ID Related Accession (Same CDS sequence) NM_173060.3 , NM_173060.4
    Accession Version NM_173060.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AL832349.1, AL832174.1, AY189741.1 and AC008906.5. On Aug 30, 2016 this sequence version replaced NM_173060.3. Transcript Variant: This variant (2) represents use of an alternate promoter compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832349.1, SRR1803617.153476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT
    TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG
    TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT
    CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG
    AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA
    GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA
    GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA
    CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT
    CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA
    GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT
    GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG
    GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG
    AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC
    AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA
    TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG
    CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA
    CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG
    GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGCAG
    AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG
    CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA
    CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG
    GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG
    AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC
    TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC
    TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT
    TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_775083.1
    CDS296..2356
    Translation

    Target ORF information:

    RefSeq Version NM_173060.4
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_173060.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT
    TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG
    TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT
    CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG
    AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA
    GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA
    GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA
    CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT
    CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA
    GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT
    GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG
    GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG
    AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC
    AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA
    TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG
    CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA
    CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG
    GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGCAG
    AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG
    CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA
    CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG
    GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG
    AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC
    TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC
    TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT
    TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39740
    Clone ID Related Accession (Same CDS sequence) NM_001042444.2
    Accession Version NM_001042444.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform j
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714711.3, NM_001042444.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.8993.1, SRR1803612.218850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC
    TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT
    GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA
    GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG
    GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG
    CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA
    GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA
    AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG
    CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT
    AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT
    CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG
    CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG
    GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG
    GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT
    AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA
    CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT
    CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA
    GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG
    CAGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG
    CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT
    GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC
    CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA
    AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT
    CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC
    AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA
    ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035909.1
    CDS296..2299
    Translation

    Target ORF information:

    RefSeq Version NM_001042444.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042444.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC
    TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT
    GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA
    GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG
    GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG
    CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA
    GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA
    AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG
    CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT
    AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT
    CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG
    CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG
    GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG
    GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT
    AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA
    CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT
    CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA
    GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG
    CAGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG
    CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT
    GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC
    CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA
    AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT
    CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC
    AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA
    ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39744
    Clone ID Related Accession (Same CDS sequence) XM_006714715.3
    Accession Version XM_006714715.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X44
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT 
    GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA
    GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG
    GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA
    TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA
    TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG
    GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG
    AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA
    GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA
    GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG
    GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC
    ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT
    TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA
    ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA
    GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA
    GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC
    AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT
    GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA
    GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA
    GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA
    CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA
    AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714778.1
    CDS838..2109
    Translation

    Target ORF information:

    RefSeq Version XM_006714715.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X47, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714715.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT 
    GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA
    GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG
    GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA
    TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA
    TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG
    GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG
    AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA
    GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA
    GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG
    GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC
    ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT
    TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA
    ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA
    GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA
    GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC
    AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT
    GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA
    GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA
    GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA
    CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA
    AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21399
    Clone ID Related Accession (Same CDS sequence) NM_001284213.3
    Accession Version NM_001284213.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product calpastatin isoform o
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AK300624.1, KC877037.1, BQ940454.1, CA775469.1 and BM676369.1. On Aug 30, 2016 this sequence version replaced NM_001284213.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300624.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGTCAGGC
    ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA
    GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG
    GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAAAAG
    GAAGGGATCA CAGGGCCTCC TGCAGACTCT TCGAAACCCA TAGGGCCAGA TGATGCTATA
    GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA
    AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT
    CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG
    GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT
    AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT
    GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA
    TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT
    TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA
    GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG
    TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT
    GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA
    CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA
    AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG
    CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG
    GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT
    GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA
    GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001271142.1
    CDS296..2206
    Translation

    Target ORF information:

    RefSeq Version NM_001284213.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001284213.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGTCAGGC
    ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA
    GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG
    GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAAAAG
    GAAGGGATCA CAGGGCCTCC TGCAGACTCT TCGAAACCCA TAGGGCCAGA TGATGCTATA
    GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA
    AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT
    CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG
    GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT
    AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT
    GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA
    TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT
    TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA
    GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG
    TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT
    GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA
    CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA
    AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG
    CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG
    GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT
    GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA
    GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39741
    Clone ID Related Accession (Same CDS sequence) NM_001042446.2
    Accession Version NM_001042446.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product calpastatin isoform l
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714712.3, NM_001042446.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.66674.1, SRR1803614.37703.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA CCCATAGGGC CAGATGATGC TATAGACGCC
    TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG
    GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC
    CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG
    TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA
    GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA
    ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA
    GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA
    GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA
    TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT
    ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA
    GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG
    ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA
    GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC
    CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT
    TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA
    CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA
    GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC
    TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA
    GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC
    CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT
    ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC
    TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT
    AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT
    TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035911.1
    CDS296..2260
    Translation

    Target ORF information:

    RefSeq Version NM_001042446.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042446.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA CCCATAGGGC CAGATGATGC TATAGACGCC
    TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG
    GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC
    CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG
    TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA
    GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA
    ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA
    GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA
    GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA
    TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT
    ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA
    GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG
    ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA
    GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC
    CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT
    TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA
    CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA
    GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC
    TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA
    GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC
    CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT
    ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC
    TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT
    AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT
    TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39742
    Clone ID Related Accession (Same CDS sequence) XM_006714713.3
    Accession Version XM_006714713.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X42
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCAGT TTCTATCTTC GACTTTCTTG GAGGGCTCAC CGGCCACAGT GTGGCACGAT 
    AAGCTTTGTG ACGGTGAACG CAGAGGAGCA AGAGAAGCAG TTCGTATCTT CCAGGACCAA
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714776.1
    CDS88..1383
    Translation

    Target ORF information:

    RefSeq Version XM_006714713.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X45, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714713.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCAGT TTCTATCTTC GACTTTCTTG GAGGGCTCAC CGGCCACAGT GTGGCACGAT 
    AAGCTTTGTG ACGGTGAACG CAGAGGAGCA AGAGAAGCAG TTCGTATCTT CCAGGACCAA
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39743
    Clone ID Related Accession (Same CDS sequence) XM_006714714.3
    Accession Version XM_006714714.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1293bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X43
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCAGT TTCTATCTTC GACTTTCTTG GAGGGCTCAC CGGCCACAGT GTGGCACGAT 
    AAGCTTTGTG ACGGTGAACG CAGAGGAGCA AGAGAAGCAG TTCGTATCTT CCAGGACCAA
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714777.1
    CDS88..1380
    Translation

    Target ORF information:

    RefSeq Version XM_006714714.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X46, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714714.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGCCAGT TTCTATCTTC GACTTTCTTG GAGGGCTCAC CGGCCACAGT GTGGCACGAT 
    AAGCTTTGTG ACGGTGAACG CAGAGGAGCA AGAGAAGCAG TTCGTATCTT CCAGGACCAA
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56646
    Clone ID Related Accession (Same CDS sequence) XM_011543658.2
    Accession Version XM_011543658.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X39
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGAAAAAA
    GAAAAGAAAT CATTAACCCC AGCTGTGCCA GTTGAATCTA AACCGGATAA ACCATCGGGA
    AAGTCAGGCA TGGATGCTGC TTTGGATGAC TTAATAGATA CTTTAGGAGG ACCTGAAGAA
    ACTGAAGAAG AAAATACAAC GTATACTGGA CCAGAAGTTT CAGATCCAAT GAGTTCCACC
    TACATAGAGG AATTGGGTAA AAGAGAAGTC ACAATTCCTC CAAAATATAG GGAACTATTG
    GCTAAAAAGG AAGGGATCAC AGGGCCTCCT GCAGACTCTT CGAAACCCAT AGGGCCAGAT
    GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG
    AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA
    AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA
    GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC
    CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT
    GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA
    AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT
    GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT
    GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG
    ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG
    CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA
    GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG
    CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA
    GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC
    TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA
    TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA
    ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT
    GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA
    CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA
    AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA
    CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC
    CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG
    AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA
    GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541960.1
    CDS169..2148
    Translation

    Target ORF information:

    RefSeq Version XM_011543658.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011543658.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGAAAAAA
    GAAAAGAAAT CATTAACCCC AGCTGTGCCA GTTGAATCTA AACCGGATAA ACCATCGGGA
    AAGTCAGGCA TGGATGCTGC TTTGGATGAC TTAATAGATA CTTTAGGAGG ACCTGAAGAA
    ACTGAAGAAG AAAATACAAC GTATACTGGA CCAGAAGTTT CAGATCCAAT GAGTTCCACC
    TACATAGAGG AATTGGGTAA AAGAGAAGTC ACAATTCCTC CAAAATATAG GGAACTATTG
    GCTAAAAAGG AAGGGATCAC AGGGCCTCCT GCAGACTCTT CGAAACCCAT AGGGCCAGAT
    GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG
    AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA
    AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA
    GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC
    CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT
    GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA
    AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT
    GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT
    GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG
    ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG
    CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA
    GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG
    CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA
    GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC
    TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA
    TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA
    ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT
    GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA
    CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA
    AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA
    CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC
    CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG
    AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA
    GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21220
    Clone ID Related Accession (Same CDS sequence) NM_001330631.1 , XM_006714708.2 , NM_001190442.1
    Accession Version NM_001330631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product calpastatin isoform m
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_006714708.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.23395.1, SRR1803611.253714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317560.1
    CDS296..2383
    Translation

    Target ORF information:

    RefSeq Version NM_001330631.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21220
    Clone ID Related Accession (Same CDS sequence) NM_001330631.1 , XM_006714708.2 , NM_001190442.1
    Accession Version NM_001190442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product calpastatin isoform m
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB450697.1, AC020900.5, AB208816.1, AC008906.5, AL832349.1 and AY189741.1. This sequence is a reference standard in the RefSeqGene project. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177371.1
    CDS304..2391
    Translation

    Target ORF information:

    RefSeq Version NM_001190442.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39736
    Clone ID Related Accession (Same CDS sequence) NM_001330628.1 , NM_001330628.2
    Accession Version NM_001330628.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product calpastatin isoform r
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_006714706.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.44671.1, SRR1803614.155067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA
    GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA
    GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT
    GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA
    TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG
    GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA
    AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA
    TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAAAAGGAA
    GGGATCACAG GGCCTCCTGC AGACTCTTCG AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317557.1
    CDS159..2366
    Translation

    Target ORF information:

    RefSeq Version NM_001330628.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA
    GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA
    GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT
    GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA
    TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG
    GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA
    AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA
    TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAAAAGGAA
    GGGATCACAG GGCCTCCTGC AGACTCTTCG AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39736
    Clone ID Related Accession (Same CDS sequence) NM_001330628.1 , NM_001330628.2
    Accession Version NM_001330628.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform r
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001330628.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.44671.1, SRR1803614.155067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA
    GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA
    GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT
    GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA
    TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG
    GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA
    AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA
    TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAAAAGGAA
    GGGATCACAG GGCCTCCTGC AGACTCTTCG AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317557.1
    CDS113..2320
    Translation

    Target ORF information:

    RefSeq Version NM_001330628.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330628.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA
    GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA
    GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT
    GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA
    TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG
    GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA
    AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA
    TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAAAAGGAA
    GGGATCACAG GGCCTCCTGC AGACTCTTCG AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39737
    Clone ID Related Accession (Same CDS sequence) NM_001042443.2
    Accession Version NM_001042443.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714707.3, NM_001042443.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.140040.1, SRR1803612.274671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAAAAGGAAG GGATCACAGG GCCTCCTGCA
    GACTCTTCGA AACCCATAGG GCCAGATGAT GCTATAGACG CCTTGTCATC TGACTTCACC
    TGTGGGTCGC CTACAGCTGC TGGAAAGAAA ACTGAAAAAG AGGAATCTAC AGAAGTTTTA
    AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG
    GTGGAGAAGG ATACAATGAG TGATCAAGCA CTCGAGGCTC TGTCGGCTTC ACTGGGCACC
    CGGCAAGCAG AACCTGAGCT CGACCTCCGC TCAATTAAGG AAGTCGATGA GGCAAAAGCT
    AAAGAAGAAA AACTAGAGAA GTGTGGTGAG GATGATGAAA CAATCCCATC TGAGTACAGA
    TTAAAACCAG CCACGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAAAACCC
    AAGCCTCGGA GTGAATCAGA ACTCATTGAT GAACTTTCAG AAGATTTTGA CCGGTCTGAA
    TGTAAAGAGA AACCATCTAA GCCAACTGAA AAGACAGAAG AATCTAAGGC CGCTGCTCCA
    GCTCCTGTGT CGGAGGCTGT GTGTCGGACC TCCATGTGTA GTATACAGTC AGCACCCCCT
    GAGCCGGCTA CCTTGAAGGG CACAGTGCCA GATGATGCTG TAGAAGCCTT GGCTGATAGC
    CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT GGAAAACCTG TGATGGATAA AGTCAAGGAG
    AAGGCCAAAG AAGAAGACCG TGAAAAGCTT GGTGAAAAAG AAGAAACAAT TCCTCCTGAT
    TATAGATTAG AAGAGGTCAA GGATAAAGAT GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG
    GAACAGCTTC CACCCATGAG TGAAGACTTC CTTCTGGATG CTTTGTCTGA GGACTTCTCT
    GGTCCACAAA ATGCTTCATC TCTTAAATTT GAAGATGCTA AACTTGCTGC TGCCATCTCT
    GAAGTGGTTT CCCAAACCCC AGCTTCAACG ACCCAAGCTG GAGCCCCACC CCGTGATACC
    TCGCAGAGTG ACAAAGACCT CGATGATGCC TTGGATAAAC TCTCTGACAG TCTAGGACAA
    AGGCAGCCTG ACCCAGATGA GAACAAACCA ATGGAAGATA AAGTAAAGGA AAAAGCTAAA
    GCTGAACATA GAGACAAGCT TGGAGAAAGA GATGACACTA TCCCACCTGA ATACAGACAT
    CTCCTGGATG ATAATGGACA GGACAAACCA GTGAAGCCAC CTACAAAGAA ATCAGAGGAT
    TCAAAGAAAC CTGCAGATGA CCAAGACCCC ATTGATGCTC TCTCAGGAGA TCTGGACAGC
    TGTCCCTCCA CTACAGAAAC CTCACAGAAC ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT
    TCCAGCTCCA AAGCACCTAA GAATGGAGGT AAAGCGAAGG ATTCAGCAAA GACAACAGAG
    GAAACTTCCA AGCCAAAAGA TGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035908.1
    CDS296..2422
    Translation

    Target ORF information:

    RefSeq Version NM_001042443.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042443.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAAAAGGAAG GGATCACAGG GCCTCCTGCA
    GACTCTTCGA AACCCATAGG GCCAGATGAT GCTATAGACG CCTTGTCATC TGACTTCACC
    TGTGGGTCGC CTACAGCTGC TGGAAAGAAA ACTGAAAAAG AGGAATCTAC AGAAGTTTTA
    AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG
    GTGGAGAAGG ATACAATGAG TGATCAAGCA CTCGAGGCTC TGTCGGCTTC ACTGGGCACC
    CGGCAAGCAG AACCTGAGCT CGACCTCCGC TCAATTAAGG AAGTCGATGA GGCAAAAGCT
    AAAGAAGAAA AACTAGAGAA GTGTGGTGAG GATGATGAAA CAATCCCATC TGAGTACAGA
    TTAAAACCAG CCACGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAAAACCC
    AAGCCTCGGA GTGAATCAGA ACTCATTGAT GAACTTTCAG AAGATTTTGA CCGGTCTGAA
    TGTAAAGAGA AACCATCTAA GCCAACTGAA AAGACAGAAG AATCTAAGGC CGCTGCTCCA
    GCTCCTGTGT CGGAGGCTGT GTGTCGGACC TCCATGTGTA GTATACAGTC AGCACCCCCT
    GAGCCGGCTA CCTTGAAGGG CACAGTGCCA GATGATGCTG TAGAAGCCTT GGCTGATAGC
    CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT GGAAAACCTG TGATGGATAA AGTCAAGGAG
    AAGGCCAAAG AAGAAGACCG TGAAAAGCTT GGTGAAAAAG AAGAAACAAT TCCTCCTGAT
    TATAGATTAG AAGAGGTCAA GGATAAAGAT GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG
    GAACAGCTTC CACCCATGAG TGAAGACTTC CTTCTGGATG CTTTGTCTGA GGACTTCTCT
    GGTCCACAAA ATGCTTCATC TCTTAAATTT GAAGATGCTA AACTTGCTGC TGCCATCTCT
    GAAGTGGTTT CCCAAACCCC AGCTTCAACG ACCCAAGCTG GAGCCCCACC CCGTGATACC
    TCGCAGAGTG ACAAAGACCT CGATGATGCC TTGGATAAAC TCTCTGACAG TCTAGGACAA
    AGGCAGCCTG ACCCAGATGA GAACAAACCA ATGGAAGATA AAGTAAAGGA AAAAGCTAAA
    GCTGAACATA GAGACAAGCT TGGAGAAAGA GATGACACTA TCCCACCTGA ATACAGACAT
    CTCCTGGATG ATAATGGACA GGACAAACCA GTGAAGCCAC CTACAAAGAA ATCAGAGGAT
    TCAAAGAAAC CTGCAGATGA CCAAGACCCC ATTGATGCTC TCTCAGGAGA TCTGGACAGC
    TGTCCCTCCA CTACAGAAAC CTCACAGAAC ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT
    TCCAGCTCCA AAGCACCTAA GAATGGAGGT AAAGCGAAGG ATTCAGCAAA GACAACAGAG
    GAAACTTCCA AGCCAAAAGA TGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39738
    Clone ID Related Accession (Same CDS sequence) NM_001330633.1
    Accession Version NM_001330633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product calpastatin isoform v
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_006714709.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.166555.1, SRR1660809.94270.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317562.1
    CDS296..2365
    Translation

    Target ORF information:

    RefSeq Version NM_001330633.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39739
    Clone ID Related Accession (Same CDS sequence) NM_001042445.2
    Accession Version NM_001042445.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2022bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform k
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714710.3, NM_001042445.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.102275.1, SRR1660803.123392.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAACCC ATAGGGCCAG ATGATGCTAT AGACGCCTTG
    TCATCTGACT TCACCTGTGG GTCGCCTACA GCTGCTGGAA AGAAAACTGA AAAAGAGGAA
    TCTACAGAAG TTTTAAAAGC TCAGTCAGCA GGGACAGTCA GAAGTGCTGC TCCACCCCAA
    GAGAAGAAAA GAAAGGTGGA GAAGGATACA ATGAGTGATC AAGCACTCGA GGCTCTGTCG
    GCTTCACTGG GCACCCGGCA AGCAGAACCT GAGCTCGACC TCCGCTCAAT TAAGGAAGTC
    GATGAGGCAA AAGCTAAAGA AGAAAAACTA GAGAAGTGTG GTGAGGATGA TGAAACAATC
    CCATCTGAGT ACAGATTAAA ACCAGCCACG GATAAAGATG GAAAACCACT ATTGCCAGAG
    CCTGAAGAAA AACCCAAGCC TCGGAGTGAA TCAGAACTCA TTGATGAACT TTCAGAAGAT
    TTTGACCGGT CTGAATGTAA AGAGAAACCA TCTAAGCCAA CTGAAAAGAC AGAAGAATCT
    AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG GCTGTGTGTC GGACCTCCAT GTGTAGTATA
    CAGTCAGCAC CCCCTGAGCC GGCTACCTTG AAGGGCACAG TGCCAGATGA TGCTGTAGAA
    GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA GCAGATCCAG AAGATGGAAA ACCTGTGATG
    GATAAAGTCA AGGAGAAGGC CAAAGAAGAA GACCGTGAAA AGCTTGGTGA AAAAGAAGAA
    ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA GCCACTCCTG
    CCAAAAGAGT CTAAGGAACA GCTTCCACCC ATGAGTGAAG ACTTCCTTCT GGATGCTTTG
    TCTGAGGACT TCTCTGGTCC ACAAAATGCT TCATCTCTTA AATTTGAAGA TGCTAAACTT
    GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTT CAACGACCCA AGCTGGAGCC
    CCACCCCGTG ATACCTCGCA GAGTGACAAA GACCTCGATG ATGCCTTGGA TAAACTCTCT
    GACAGTCTAG GACAAAGGCA GCCTGACCCA GATGAGAACA AACCAATGGA AGATAAAGTA
    AAGGAAAAAG CTAAAGCTGA ACATAGAGAC AAGCTTGGAG AAAGAGATGA CACTATCCCA
    CCTGAATACA GACATCTCCT GGATGATAAT GGACAGGACA AACCAGTGAA GCCACCTACA
    AAGAAATCAG AGGATTCAAA GAAACCTGCA GATGACCAAG ACCCCATTGA TGCTCTCTCA
    GGAGATCTGG ACAGCTGTCC CTCCACTACA GAAACCTCAC AGAACACAGC AAAGGATAAG
    TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA CCTAAGAATG GAGGTAAAGC GAAGGATTCA
    GCAAAGACAA CAGAGGAAAC TTCCAAGCCA AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035910.1
    CDS296..2317
    Translation

    Target ORF information:

    RefSeq Version NM_001042445.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042445.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAACCC ATAGGGCCAG ATGATGCTAT AGACGCCTTG
    TCATCTGACT TCACCTGTGG GTCGCCTACA GCTGCTGGAA AGAAAACTGA AAAAGAGGAA
    TCTACAGAAG TTTTAAAAGC TCAGTCAGCA GGGACAGTCA GAAGTGCTGC TCCACCCCAA
    GAGAAGAAAA GAAAGGTGGA GAAGGATACA ATGAGTGATC AAGCACTCGA GGCTCTGTCG
    GCTTCACTGG GCACCCGGCA AGCAGAACCT GAGCTCGACC TCCGCTCAAT TAAGGAAGTC
    GATGAGGCAA AAGCTAAAGA AGAAAAACTA GAGAAGTGTG GTGAGGATGA TGAAACAATC
    CCATCTGAGT ACAGATTAAA ACCAGCCACG GATAAAGATG GAAAACCACT ATTGCCAGAG
    CCTGAAGAAA AACCCAAGCC TCGGAGTGAA TCAGAACTCA TTGATGAACT TTCAGAAGAT
    TTTGACCGGT CTGAATGTAA AGAGAAACCA TCTAAGCCAA CTGAAAAGAC AGAAGAATCT
    AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG GCTGTGTGTC GGACCTCCAT GTGTAGTATA
    CAGTCAGCAC CCCCTGAGCC GGCTACCTTG AAGGGCACAG TGCCAGATGA TGCTGTAGAA
    GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA GCAGATCCAG AAGATGGAAA ACCTGTGATG
    GATAAAGTCA AGGAGAAGGC CAAAGAAGAA GACCGTGAAA AGCTTGGTGA AAAAGAAGAA
    ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA GCCACTCCTG
    CCAAAAGAGT CTAAGGAACA GCTTCCACCC ATGAGTGAAG ACTTCCTTCT GGATGCTTTG
    TCTGAGGACT TCTCTGGTCC ACAAAATGCT TCATCTCTTA AATTTGAAGA TGCTAAACTT
    GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTT CAACGACCCA AGCTGGAGCC
    CCACCCCGTG ATACCTCGCA GAGTGACAAA GACCTCGATG ATGCCTTGGA TAAACTCTCT
    GACAGTCTAG GACAAAGGCA GCCTGACCCA GATGAGAACA AACCAATGGA AGATAAAGTA
    AAGGAAAAAG CTAAAGCTGA ACATAGAGAC AAGCTTGGAG AAAGAGATGA CACTATCCCA
    CCTGAATACA GACATCTCCT GGATGATAAT GGACAGGACA AACCAGTGAA GCCACCTACA
    AAGAAATCAG AGGATTCAAA GAAACCTGCA GATGACCAAG ACCCCATTGA TGCTCTCTCA
    GGAGATCTGG ACAGCTGTCC CTCCACTACA GAAACCTCAC AGAACACAGC AAAGGATAAG
    TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA CCTAAGAATG GAGGTAAAGC GAAGGATTCA
    GCAAAGACAA CAGAGGAAAC TTCCAAGCCA AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21220
    Clone ID Related Accession (Same CDS sequence) NM_001330631.1 , XM_006714708.2 , NM_001190442.1
    Accession Version XM_006714708.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product calpastatin isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714771.1
    Translation

    Target ORF information:

    RefSeq Version XM_006714708.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714708.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA
    CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT
    AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT
    ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA
    CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT
    CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG
    GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG
    GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39729
    Clone ID Related Accession (Same CDS sequence) XM_006714699.3
    Accession Version XM_006714699.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC
    CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA
    ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC
    CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT
    ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA
    TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG
    AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA
    TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC
    ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA
    GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG
    GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAAAAG
    GAAGGGATCA CAGGGCCTCC TGCAGACTCT TCGAAACCCA TAGGGCCAGA TGATGCTATA
    GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA
    AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT
    CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG
    GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT
    AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT
    GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA
    TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT
    TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA
    GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG
    TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT
    GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA
    CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA
    AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG
    CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG
    GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT
    GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA
    GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714762.1
    CDS144..2474
    Translation

    Target ORF information:

    RefSeq Version XM_006714699.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714699.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC
    CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA
    ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC
    CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT
    ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA
    TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG
    AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA
    TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC
    ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA
    GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG
    GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAAAAG
    GAAGGGATCA CAGGGCCTCC TGCAGACTCT TCGAAACCCA TAGGGCCAGA TGATGCTATA
    GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA
    AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT
    CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG
    GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT
    AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT
    GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA
    TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT
    TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA
    GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG
    TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT
    GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA
    CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA
    AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG
    CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG
    GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT
    GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA
    GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39732
    Clone ID Related Accession (Same CDS sequence) XM_006714702.3
    Accession Version XM_006714702.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714702.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC
    CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA
    ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCCAAAAA GCCTACCCAA GCAGGCATCA
    GATACAGGAA GTAACGATGC TCACAATAAA AAAGCAGTTT CCAGATCAGC TGAACAGCAG
    CCATCAGAGA AATCAACAGA ACCAAAGACT AAACCACAAG ACATGATTTC TGCTGGTGGA
    GAGAGTGTTG CTGGTATCAC TGCAATATCT GGCAAGCCGG GTGACAAGAA AAAAGAAAAG
    AAATCATTAA CCCCAGCTGT GCCAGTTGAA TCTAAACCGG ATAAACCATC GGGAAAGTCA
    GGCATGGATG CTGCTTTGGA TGACTTAATA GATACTTTAG GAGGACCTGA AGAAACTGAA
    GAAGAAAATA CAACGTATAC TGGACCAGAA GTTTCAGATC CAATGAGTTC CACCTACATA
    GAGGAATTGG GTAAAAGAGA AGTCACAATT CCTCCAAAAT ATAGGGAACT ATTGGCTAAA
    AAGGAAGGGA TCACAGGGCC TCCTGCAGAC TCTTCGAAAC CCATAGGGCC AGATGATGCT
    ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT
    GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT
    GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC
    GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA
    ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT
    GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA
    CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA
    CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG
    ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC
    ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT
    GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA
    AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT
    GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA
    AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT
    CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA
    GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC
    CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA TGATGCCTTG
    GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG
    GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT
    GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG
    AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT
    GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA
    GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA
    GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714765.1
    CDS112..2385
    Translation

    Target ORF information:

    RefSeq Version XM_006714702.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714702.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC
    CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA
    ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCCAAAAA GCCTACCCAA GCAGGCATCA
    GATACAGGAA GTAACGATGC TCACAATAAA AAAGCAGTTT CCAGATCAGC TGAACAGCAG
    CCATCAGAGA AATCAACAGA ACCAAAGACT AAACCACAAG ACATGATTTC TGCTGGTGGA
    GAGAGTGTTG CTGGTATCAC TGCAATATCT GGCAAGCCGG GTGACAAGAA AAAAGAAAAG
    AAATCATTAA CCCCAGCTGT GCCAGTTGAA TCTAAACCGG ATAAACCATC GGGAAAGTCA
    GGCATGGATG CTGCTTTGGA TGACTTAATA GATACTTTAG GAGGACCTGA AGAAACTGAA
    GAAGAAAATA CAACGTATAC TGGACCAGAA GTTTCAGATC CAATGAGTTC CACCTACATA
    GAGGAATTGG GTAAAAGAGA AGTCACAATT CCTCCAAAAT ATAGGGAACT ATTGGCTAAA
    AAGGAAGGGA TCACAGGGCC TCCTGCAGAC TCTTCGAAAC CCATAGGGCC AGATGATGCT
    ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT
    GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT
    GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC
    GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA
    ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT
    GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA
    CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA
    CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG
    ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC
    ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT
    GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA
    AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT
    GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA
    AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT
    CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA
    GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC
    CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA TGATGCCTTG
    GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG
    GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT
    GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG
    AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT
    GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA
    GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA
    GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39734
    Clone ID Related Accession (Same CDS sequence) XM_006714704.3 , NM_001375317.1
    Accession Version XM_006714704.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT
    GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA
    ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT
    GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT
    ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG
    GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA AAAGGAAGGG
    ATCACAGGGC CTCCTGCAGA CTCTTCGAAA CCCATAGGGC CAGATGATGC TATAGACGCC
    TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG
    GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC
    CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG
    TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA
    GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA
    ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA
    GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA
    GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA
    TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT
    ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA
    GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG
    ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA
    GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC
    CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT
    TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA
    CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA
    GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC
    TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA
    GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC
    CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT
    ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC
    TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT
    AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT
    TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714767.1
    CDS154..2418
    Translation

    Target ORF information:

    RefSeq Version XM_006714704.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714704.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT
    GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA
    ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT
    GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT
    ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG
    GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA AAAGGAAGGG
    ATCACAGGGC CTCCTGCAGA CTCTTCGAAA CCCATAGGGC CAGATGATGC TATAGACGCC
    TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG
    GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC
    CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG
    TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA
    GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA
    ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA
    GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA
    GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA
    TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT
    ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA
    GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG
    ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA
    GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC
    CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT
    TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA
    CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA
    GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC
    TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA
    GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC
    CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT
    ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC
    TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT
    AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT
    TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56645
    Clone ID Related Accession (Same CDS sequence) XM_011543657.2
    Accession Version XM_011543657.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC
    TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT
    GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA
    GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG
    GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG
    CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA
    GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA
    AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG
    CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT
    AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT
    CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG
    CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG
    GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG
    GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT
    AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA
    CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT
    CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA
    GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG
    CAGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG
    CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT
    GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC
    CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA
    AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT
    CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC
    AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA
    ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541959.1
    CDS112..2295
    Translation

    Target ORF information:

    RefSeq Version XM_011543657.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011543657.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC
    TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT
    GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA
    GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG
    GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG
    CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA
    GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA
    AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG
    CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT
    AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT
    CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG
    CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG
    GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG
    GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT
    AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA
    CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT
    CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA
    GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG
    CAGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG
    CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT
    GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC
    CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA
    AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT
    CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC
    AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA
    ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102190
    Clone ID Related Accession (Same CDS sequence) NM_001330630.1
    Accession Version NM_001330630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform t
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009932.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.205463.1, SRR1803616.10936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA CCCATAGGGC CAGATGATGC TATAGACGCC
    TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG
    GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC
    CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG
    TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA
    GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA
    ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA
    GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA
    GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA
    TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT
    ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA
    GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG
    ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA
    GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC
    CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT
    TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA
    CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA
    GCCCCACCCC GTGATACCTC GAGTGACAAA GACCTCGATG ATGCCTTGGA TAAACTCTCT
    GACAGTCTAG GACAAAGGCA GCCTGACCCA GATGAGAACA AACCAATGGA AGATAAAGTA
    AAGGAAAAAG CTAAAGCTGA ACATAGAGAC AAGCTTGGAG AAAGAGATGA CACTATCCCA
    CCTGAATACA GACATCTCCT GGATGATAAT GGACAGGACA AACCAGTGAA GCCACCTACA
    AAGAAATCAG AGGATTCAAA GAAACCTGCA GATGACCAAG ACCCCATTGA TGCTCTCTCA
    GGAGATCTGG ACAGCTGTCC CTCCACTACA GAAACCTCAC AGAACACAGC AAAGGATAAG
    TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA CCTAAGAATG GAGGTAAAGC GAAGGATTCA
    GCAAAGACAA CAGAGGAAAC TTCCAAGCCA AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317559.1
    CDS296..2257
    Translation

    Target ORF information:

    RefSeq Version NM_001330630.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA
    AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT
    AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT
    GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA
    TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA
    GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA
    GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT
    CCTCCAAAAT ATAGGGAACT ATTGGCTAAA CCCATAGGGC CAGATGATGC TATAGACGCC
    TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG
    GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC
    CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG
    TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA
    GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA
    ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA
    GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA
    GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA
    TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT
    ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA
    GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG
    ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA
    GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC
    CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT
    TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA
    CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA
    GCCCCACCCC GTGATACCTC GAGTGACAAA GACCTCGATG ATGCCTTGGA TAAACTCTCT
    GACAGTCTAG GACAAAGGCA GCCTGACCCA GATGAGAACA AACCAATGGA AGATAAAGTA
    AAGGAAAAAG CTAAAGCTGA ACATAGAGAC AAGCTTGGAG AAAGAGATGA CACTATCCCA
    CCTGAATACA GACATCTCCT GGATGATAAT GGACAGGACA AACCAGTGAA GCCACCTACA
    AAGAAATCAG AGGATTCAAA GAAACCTGCA GATGACCAAG ACCCCATTGA TGCTCTCTCA
    GGAGATCTGG ACAGCTGTCC CTCCACTACA GAAACCTCAC AGAACACAGC AAAGGATAAG
    TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA CCTAAGAATG GAGGTAAAGC GAAGGATTCA
    GCAAAGACAA CAGAGGAAAC TTCCAAGCCA AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102283
    Clone ID Related Accession (Same CDS sequence) NM_001330632.1
    Accession Version NM_001330632.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform u
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009929.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.176286.1, SRR1660803.69084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT
    TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG
    TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT
    CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG
    AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA
    GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA
    GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA
    CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT
    CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA
    GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT
    GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG
    GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG
    AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC
    AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA
    TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG
    CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA
    CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG
    GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGAGT
    GACAAAGACC TCGATGATGC CTTGGATAAA CTCTCTGACA GTCTAGGACA AAGGCAGCCT
    GACCCAGATG AGAACAAACC AATGGAAGAT AAAGTAAAGG AAAAAGCTAA AGCTGAACAT
    AGAGACAAGC TTGGAGAAAG AGATGACACT ATCCCACCTG AATACAGACA TCTCCTGGAT
    GATAATGGAC AGGACAAACC AGTGAAGCCA CCTACAAAGA AATCAGAGGA TTCAAAGAAA
    CCTGCAGATG ACCAAGACCC CATTGATGCT CTCTCAGGAG ATCTGGACAG CTGTCCCTCC
    ACTACAGAAA CCTCACAGAA CACAGCAAAG GATAAGTGCA AGAAGGCTGC TTCCAGCTCC
    AAAGCACCTA AGAATGGAGG TAAAGCGAAG GATTCAGCAA AGACAACAGA GGAAACTTCC
    AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317561.1
    CDS296..2353
    Translation

    Target ORF information:

    RefSeq Version NM_001330632.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330632.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT
    TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG
    TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT
    CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG
    AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA
    GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA
    GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA
    CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT
    CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA
    GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT
    GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG
    GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG
    AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC
    AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA
    TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG
    CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA
    CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG
    GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGAGT
    GACAAAGACC TCGATGATGC CTTGGATAAA CTCTCTGACA GTCTAGGACA AAGGCAGCCT
    GACCCAGATG AGAACAAACC AATGGAAGAT AAAGTAAAGG AAAAAGCTAA AGCTGAACAT
    AGAGACAAGC TTGGAGAAAG AGATGACACT ATCCCACCTG AATACAGACA TCTCCTGGAT
    GATAATGGAC AGGACAAACC AGTGAAGCCA CCTACAAAGA AATCAGAGGA TTCAAAGAAA
    CCTGCAGATG ACCAAGACCC CATTGATGCT CTCTCAGGAG ATCTGGACAG CTGTCCCTCC
    ACTACAGAAA CCTCACAGAA CACAGCAAAG GATAAGTGCA AGAAGGCTGC TTCCAGCTCC
    AAAGCACCTA AGAATGGAGG TAAAGCGAAG GATTCAGCAA AGACAACAGA GGAAACTTCC
    AAGCCAAAAG ATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102294
    Clone ID Related Accession (Same CDS sequence) NM_001330634.1
    Accession Version NM_001330634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform w
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009930.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.44180.1, SRR1803614.182278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAACCCA TAGGGCCAGA TGATGCTATA
    GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA
    AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT
    CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG
    GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT
    AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT
    GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA
    TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT
    TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA
    GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG
    TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT
    GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA
    CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA
    AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG
    CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG
    GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT
    GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA
    GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317563.1
    CDS296..2326
    Translation

    Target ORF information:

    RefSeq Version NM_001330634.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAACCCA TAGGGCCAGA TGATGCTATA
    GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA
    AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT
    CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG
    GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT
    AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT
    GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA
    TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT
    TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA
    GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG
    TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT
    GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA
    CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA
    AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG
    CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG
    GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT
    GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA
    GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102380
    Clone ID Related Accession (Same CDS sequence) NM_001330629.1 , NM_001330629.2
    Accession Version NM_001330629.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product calpastatin isoform s
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009917.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.191419.1, SRR1803615.231733.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC
    CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA
    ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC
    CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT
    ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA
    TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG
    AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA
    TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC
    ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA
    GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG
    GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAACCC
    ATAGGGCCAG ATGATGCTAT AGACGCCTTG TCATCTGACT TCACCTGTGG GTCGCCTACA
    GCTGCTGGAA AGAAAACTGA AAAAGAGGAA TCTACAGAAG TTTTAAAAGC TCAGTCAGCA
    GGGACAGTCA GAAGTGCTGC TCCACCCCAA GAGAAGAAAA GAAAGGTGGA GAAGGATACA
    ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT
    GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA
    GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG
    GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA
    TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA
    TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG
    GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG
    AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA
    GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA
    GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG
    GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC
    ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT
    TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA
    ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA
    GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA
    GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC
    AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT
    GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA
    GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA
    GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA
    CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA
    AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317558.1
    CDS159..2450
    Translation

    Target ORF information:

    RefSeq Version NM_001330629.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC
    CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA
    ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC
    CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT
    ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA
    TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG
    AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA
    TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC
    ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA
    GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG
    GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAACCC
    ATAGGGCCAG ATGATGCTAT AGACGCCTTG TCATCTGACT TCACCTGTGG GTCGCCTACA
    GCTGCTGGAA AGAAAACTGA AAAAGAGGAA TCTACAGAAG TTTTAAAAGC TCAGTCAGCA
    GGGACAGTCA GAAGTGCTGC TCCACCCCAA GAGAAGAAAA GAAAGGTGGA GAAGGATACA
    ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT
    GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA
    GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG
    GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA
    TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA
    TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG
    GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG
    AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA
    GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA
    GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG
    GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC
    ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT
    TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA
    ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA
    GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA
    GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC
    AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT
    GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA
    GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA
    GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA
    CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA
    AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102380
    Clone ID Related Accession (Same CDS sequence) NM_001330629.1 , NM_001330629.2
    Accession Version NM_001330629.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product calpastatin isoform s
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001330629.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.231733.1, SRR1803611.191419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC
    CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA
    ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC
    CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT
    ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA
    TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG
    AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA
    TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC
    ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA
    GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG
    GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAACCC
    ATAGGGCCAG ATGATGCTAT AGACGCCTTG TCATCTGACT TCACCTGTGG GTCGCCTACA
    GCTGCTGGAA AGAAAACTGA AAAAGAGGAA TCTACAGAAG TTTTAAAAGC TCAGTCAGCA
    GGGACAGTCA GAAGTGCTGC TCCACCCCAA GAGAAGAAAA GAAAGGTGGA GAAGGATACA
    ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT
    GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA
    GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG
    GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA
    TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA
    TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG
    GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG
    AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA
    GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA
    GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG
    GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC
    ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT
    TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA
    ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA
    GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA
    GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC
    AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT
    GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA
    GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA
    GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA
    CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA
    AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317558.1
    CDS113..2404
    Translation

    Target ORF information:

    RefSeq Version NM_001330629.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330629.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC
    CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA
    ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC
    CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT
    ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA
    TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG
    AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA
    TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC
    ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA
    GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG
    GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAACCC
    ATAGGGCCAG ATGATGCTAT AGACGCCTTG TCATCTGACT TCACCTGTGG GTCGCCTACA
    GCTGCTGGAA AGAAAACTGA AAAAGAGGAA TCTACAGAAG TTTTAAAAGC TCAGTCAGCA
    GGGACAGTCA GAAGTGCTGC TCCACCCCAA GAGAAGAAAA GAAAGGTGGA GAAGGATACA
    ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT
    GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA
    GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG
    GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA
    TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA
    TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG
    GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG
    AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA
    GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA
    GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG
    GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC
    ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT
    TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA
    ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA
    GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA
    GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC
    AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT
    GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA
    GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA
    GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA
    CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA
    AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39731
    Clone ID Related Accession (Same CDS sequence) NM_001042442.3 , XM_006714701.3 , NM_001042442.2
    Accession Version NM_001042442.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Homo sapiens(human)
    Product calpastatin isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Oct 22, 2019 this sequence version replaced NM_001042442.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.62328.1, SRR1803613.68618.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035907.1
    CDS48..2357
    Translation

    Target ORF information:

    RefSeq Version NM_001042442.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042442.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39734
    Clone ID Related Accession (Same CDS sequence) XM_006714704.3 , NM_001375317.1
    Accession Version NM_001375317.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform x
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296919.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT
    GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA
    ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT
    GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT
    ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG
    GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA AAAGGAAGGG
    ATCACAGGGC CTCCTGCAGA CTCTTCGAAA CCCATAGGGC CAGATGATGC TATAGACGCC
    TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG
    GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC
    CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG
    TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA
    GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA
    ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA
    GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA
    GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA
    TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT
    ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA
    GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG
    ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA
    GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC
    CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT
    TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA
    CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA
    GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC
    TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA
    GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC
    CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT
    ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC
    TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT
    AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT
    TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362246.1
    CDS113..2377
    Translation

    Target ORF information:

    RefSeq Version NM_001375317.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375317.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT
    GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA
    ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT
    GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT
    ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG
    GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA AAAGGAAGGG
    ATCACAGGGC CTCCTGCAGA CTCTTCGAAA CCCATAGGGC CAGATGATGC TATAGACGCC
    TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG
    GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC
    CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG
    TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA
    GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA
    ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA
    GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA
    GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA
    TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT
    ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA
    GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG
    ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA
    GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC
    CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT
    TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA
    CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA
    GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC
    TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA
    GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC
    CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT
    ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC
    TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT
    AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT
    TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96116
    Clone ID Related Accession (Same CDS sequence) XM_017009920.1
    Accession Version XM_017009920.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT
    GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA
    ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT
    GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT
    ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG
    GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA ACCCATAGGG
    CCAGATGATG CTATAGACGC CTTGTCATCT GACTTCACCT GTGGGTCGCC TACAGCTGCT
    GGAAAGAAAA CTGAAAAAGA GGAATCTACA GAAGTTTTAA AAGCTCAGTC AGCAGGGACA
    GTCAGAAGTG CTGCTCCACC CCAAGAGAAG AAAAGAAAGG TGGAGAAGGA TACAATGAGT
    GATCAAGCAC TCGAGGCTCT GTCGGCTTCA CTGGGCACCC GGCAAGCAGA ACCTGAGCTC
    GACCTCCGCT CAATTAAGGA AGTCGATGAG GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG
    TGTGGTGAGG ATGATGAAAC AATCCCATCT GAGTACAGAT TAAAACCAGC CACGGATAAA
    GATGGAAAAC CACTATTGCC AGAGCCTGAA GAAAAACCCA AGCCTCGGAG TGAATCAGAA
    CTCATTGATG AACTTTCAGA AGATTTTGAC CGGTCTGAAT GTAAAGAGAA ACCATCTAAG
    CCAACTGAAA AGACAGAAGA ATCTAAGGCC GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG
    TGTCGGACCT CCATGTGTAG TATACAGTCA GCACCCCCTG AGCCGGCTAC CTTGAAGGGC
    ACAGTGCCAG ATGATGCTGT AGAAGCCTTG GCTGATAGCC TGGGGAAAAA GGAAGCAGAT
    CCAGAAGATG GAAAACCTGT GATGGATAAA GTCAAGGAGA AGGCCAAAGA AGAAGACCGT
    GAAAAGCTTG GTGAAAAAGA AGAAACAATT CCTCCTGATT ATAGATTAGA AGAGGTCAAG
    GATAAAGATG GAAAGCCACT CCTGCCAAAA GAGTCTAAGG AACAGCTTCC ACCCATGAGT
    GAAGACTTCC TTCTGGATGC TTTGTCTGAG GACTTCTCTG GTCCACAAAA TGCTTCATCT
    CTTAAATTTG AAGATGCTAA ACTTGCTGCT GCCATCTCTG AAGTGGTTTC CCAAACCCCA
    GCTTCAACGA CCCAAGCTGG AGCCCCACCC CGTGATACCT CGCAGAGTGA CAAAGACCTC
    GATGATGCCT TGGATAAACT CTCTGACAGT CTAGGACAAA GGCAGCCTGA CCCAGATGAG
    AACAAACCAA TGGAAGATAA AGTAAAGGAA AAAGCTAAAG CTGAACATAG AGACAAGCTT
    GGAGAAAGAG ATGACACTAT CCCACCTGAA TACAGACATC TCCTGGATGA TAATGGACAG
    GACAAACCAG TGAAGCCACC TACAAAGAAA TCAGAGGATT CAAAGAAACC TGCAGATGAC
    CAAGACCCCA TTGATGCTCT CTCAGGAGAT CTGGACAGCT GTCCCTCCAC TACAGAAACC
    TCACAGAACA CAGCAAAGGA TAAGTGCAAG AAGGCTGCTT CCAGCTCCAA AGCACCTAAG
    AATGGAGGTA AAGCGAAGGA TTCAGCAAAG ACAACAGAGG AAACTTCCAA GCCAAAAGAT
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865409.1
    CDS112..2337
    Translation

    Target ORF information:

    RefSeq Version XM_017009920.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009920.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT
    GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA
    ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT
    GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT
    ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG
    GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA ACCCATAGGG
    CCAGATGATG CTATAGACGC CTTGTCATCT GACTTCACCT GTGGGTCGCC TACAGCTGCT
    GGAAAGAAAA CTGAAAAAGA GGAATCTACA GAAGTTTTAA AAGCTCAGTC AGCAGGGACA
    GTCAGAAGTG CTGCTCCACC CCAAGAGAAG AAAAGAAAGG TGGAGAAGGA TACAATGAGT
    GATCAAGCAC TCGAGGCTCT GTCGGCTTCA CTGGGCACCC GGCAAGCAGA ACCTGAGCTC
    GACCTCCGCT CAATTAAGGA AGTCGATGAG GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG
    TGTGGTGAGG ATGATGAAAC AATCCCATCT GAGTACAGAT TAAAACCAGC CACGGATAAA
    GATGGAAAAC CACTATTGCC AGAGCCTGAA GAAAAACCCA AGCCTCGGAG TGAATCAGAA
    CTCATTGATG AACTTTCAGA AGATTTTGAC CGGTCTGAAT GTAAAGAGAA ACCATCTAAG
    CCAACTGAAA AGACAGAAGA ATCTAAGGCC GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG
    TGTCGGACCT CCATGTGTAG TATACAGTCA GCACCCCCTG AGCCGGCTAC CTTGAAGGGC
    ACAGTGCCAG ATGATGCTGT AGAAGCCTTG GCTGATAGCC TGGGGAAAAA GGAAGCAGAT
    CCAGAAGATG GAAAACCTGT GATGGATAAA GTCAAGGAGA AGGCCAAAGA AGAAGACCGT
    GAAAAGCTTG GTGAAAAAGA AGAAACAATT CCTCCTGATT ATAGATTAGA AGAGGTCAAG
    GATAAAGATG GAAAGCCACT CCTGCCAAAA GAGTCTAAGG AACAGCTTCC ACCCATGAGT
    GAAGACTTCC TTCTGGATGC TTTGTCTGAG GACTTCTCTG GTCCACAAAA TGCTTCATCT
    CTTAAATTTG AAGATGCTAA ACTTGCTGCT GCCATCTCTG AAGTGGTTTC CCAAACCCCA
    GCTTCAACGA CCCAAGCTGG AGCCCCACCC CGTGATACCT CGCAGAGTGA CAAAGACCTC
    GATGATGCCT TGGATAAACT CTCTGACAGT CTAGGACAAA GGCAGCCTGA CCCAGATGAG
    AACAAACCAA TGGAAGATAA AGTAAAGGAA AAAGCTAAAG CTGAACATAG AGACAAGCTT
    GGAGAAAGAG ATGACACTAT CCCACCTGAA TACAGACATC TCCTGGATGA TAATGGACAG
    GACAAACCAG TGAAGCCACC TACAAAGAAA TCAGAGGATT CAAAGAAACC TGCAGATGAC
    CAAGACCCCA TTGATGCTCT CTCAGGAGAT CTGGACAGCT GTCCCTCCAC TACAGAAACC
    TCACAGAACA CAGCAAAGGA TAAGTGCAAG AAGGCTGCTT CCAGCTCCAA AGCACCTAAG
    AATGGAGGTA AAGCGAAGGA TTCAGCAAAG ACAACAGAGG AAACTTCCAA GCCAAAAGAT
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96117
    Clone ID Related Accession (Same CDS sequence) XM_017009921.1
    Accession Version XM_017009921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT
    GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA
    ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT
    GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT
    ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG
    GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA ACCCATAGGG
    CCAGATGATG CTATAGACGC CTTGTCATCT GACTTCACCT GTGGGTCGCC TACAGCTGCT
    GGAAAGAAAA CTGAAAAAGA GGAATCTACA GAAGTTTTAA AAGCTCAGTC AGCAGGGACA
    GTCAGAAGTG CTGCTCCACC CCAAGAGAAG AAAAGAAAGG TGGAGAAGGA TACAATGAGT
    GATCAAGCAC TCGAGGCTCT GTCGGCTTCA CTGGGCACCC GGCAAGCAGA ACCTGAGCTC
    GACCTCCGCT CAATTAAGGA AGTCGATGAG GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG
    TGTGGTGAGG ATGATGAAAC AATCCCATCT GAGTACAGAT TAAAACCAGC CACGGATAAA
    GATGGAAAAC CACTATTGCC AGAGCCTGAA GAAAAACCCA AGCCTCGGAG TGAATCAGAA
    CTCATTGATG AACTTTCAGA AGATTTTGAC CGGTCTGAAT GTAAAGAGAA ACCATCTAAG
    CCAACTGAAA AGACAGAAGA ATCTAAGGCC GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG
    TGTCGGACCT CCATGTGTAG TATACAGTCA GCACCCCCTG AGCCGGCTAC CTTGAAGGGC
    ACAGTGCCAG ATGATGCTGT AGAAGCCTTG GCTGATAGCC TGGGGAAAAA GGAAGCAGAT
    CCAGAAGATG GAAAACCTGT GATGGATAAA GTCAAGGAGA AGGCCAAAGA AGAAGACCGT
    GAAAAGCTTG GTGAAAAAGA AGAAACAATT CCTCCTGATT ATAGATTAGA AGAGGTCAAG
    GATAAAGATG GAAAGCCACT CCTGCCAAAA GAGTCTAAGG AACAGCTTCC ACCCATGAGT
    GAAGACTTCC TTCTGGATGC TTTGTCTGAG GACTTCTCTG GTCCACAAAA TGCTTCATCT
    CTTAAATTTG AAGATGCTAA ACTTGCTGCT GCCATCTCTG AAGTGGTTTC CCAAACCCCA
    GCTTCAACGA CCCAAGCTGG AGCCCCACCC CGTGATACCT CGAGTGACAA AGACCTCGAT
    GATGCCTTGG ATAAACTCTC TGACAGTCTA GGACAAAGGC AGCCTGACCC AGATGAGAAC
    AAACCAATGG AAGATAAAGT AAAGGAAAAA GCTAAAGCTG AACATAGAGA CAAGCTTGGA
    GAAAGAGATG ACACTATCCC ACCTGAATAC AGACATCTCC TGGATGATAA TGGACAGGAC
    AAACCAGTGA AGCCACCTAC AAAGAAATCA GAGGATTCAA AGAAACCTGC AGATGACCAA
    GACCCCATTG ATGCTCTCTC AGGAGATCTG GACAGCTGTC CCTCCACTAC AGAAACCTCA
    CAGAACACAG CAAAGGATAA GTGCAAGAAG GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT
    GGAGGTAAAG CGAAGGATTC AGCAAAGACA ACAGAGGAAA CTTCCAAGCC AAAAGATGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865410.1
    CDS129..2351
    Translation

    Target ORF information:

    RefSeq Version XM_017009921.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA
    GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA
    AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG
    AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT
    GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA
    ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT
    GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT
    ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG
    GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA ACCCATAGGG
    CCAGATGATG CTATAGACGC CTTGTCATCT GACTTCACCT GTGGGTCGCC TACAGCTGCT
    GGAAAGAAAA CTGAAAAAGA GGAATCTACA GAAGTTTTAA AAGCTCAGTC AGCAGGGACA
    GTCAGAAGTG CTGCTCCACC CCAAGAGAAG AAAAGAAAGG TGGAGAAGGA TACAATGAGT
    GATCAAGCAC TCGAGGCTCT GTCGGCTTCA CTGGGCACCC GGCAAGCAGA ACCTGAGCTC
    GACCTCCGCT CAATTAAGGA AGTCGATGAG GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG
    TGTGGTGAGG ATGATGAAAC AATCCCATCT GAGTACAGAT TAAAACCAGC CACGGATAAA
    GATGGAAAAC CACTATTGCC AGAGCCTGAA GAAAAACCCA AGCCTCGGAG TGAATCAGAA
    CTCATTGATG AACTTTCAGA AGATTTTGAC CGGTCTGAAT GTAAAGAGAA ACCATCTAAG
    CCAACTGAAA AGACAGAAGA ATCTAAGGCC GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG
    TGTCGGACCT CCATGTGTAG TATACAGTCA GCACCCCCTG AGCCGGCTAC CTTGAAGGGC
    ACAGTGCCAG ATGATGCTGT AGAAGCCTTG GCTGATAGCC TGGGGAAAAA GGAAGCAGAT
    CCAGAAGATG GAAAACCTGT GATGGATAAA GTCAAGGAGA AGGCCAAAGA AGAAGACCGT
    GAAAAGCTTG GTGAAAAAGA AGAAACAATT CCTCCTGATT ATAGATTAGA AGAGGTCAAG
    GATAAAGATG GAAAGCCACT CCTGCCAAAA GAGTCTAAGG AACAGCTTCC ACCCATGAGT
    GAAGACTTCC TTCTGGATGC TTTGTCTGAG GACTTCTCTG GTCCACAAAA TGCTTCATCT
    CTTAAATTTG AAGATGCTAA ACTTGCTGCT GCCATCTCTG AAGTGGTTTC CCAAACCCCA
    GCTTCAACGA CCCAAGCTGG AGCCCCACCC CGTGATACCT CGAGTGACAA AGACCTCGAT
    GATGCCTTGG ATAAACTCTC TGACAGTCTA GGACAAAGGC AGCCTGACCC AGATGAGAAC
    AAACCAATGG AAGATAAAGT AAAGGAAAAA GCTAAAGCTG AACATAGAGA CAAGCTTGGA
    GAAAGAGATG ACACTATCCC ACCTGAATAC AGACATCTCC TGGATGATAA TGGACAGGAC
    AAACCAGTGA AGCCACCTAC AAAGAAATCA GAGGATTCAA AGAAACCTGC AGATGACCAA
    GACCCCATTG ATGCTCTCTC AGGAGATCTG GACAGCTGTC CCTCCACTAC AGAAACCTCA
    CAGAACACAG CAAAGGATAA GTGCAAGAAG GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT
    GGAGGTAAAG CGAAGGATTC AGCAAAGACA ACAGAGGAAA CTTCCAAGCC AAAAGATGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96121
    Clone ID Related Accession (Same CDS sequence) XM_017009925.1
    Accession Version XM_017009925.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA
    GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA
    GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT
    GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA
    TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG
    GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA
    AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA
    TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAACCCATA
    GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT
    GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG
    ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG
    AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG
    CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG
    AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT
    AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA
    GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT
    AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT
    GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG
    GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA
    GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC
    CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC
    AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG
    AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA
    TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC
    CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGCAGAG TGACAAAGAC
    CTCGATGATG CCTTGGATAA ACTCTCTGAC AGTCTAGGAC AAAGGCAGCC TGACCCAGAT
    GAGAACAAAC CAATGGAAGA TAAAGTAAAG GAAAAAGCTA AAGCTGAACA TAGAGACAAG
    CTTGGAGAAA GAGATGACAC TATCCCACCT GAATACAGAC ATCTCCTGGA TGATAATGGA
    CAGGACAAAC CAGTGAAGCC ACCTACAAAG AAATCAGAGG ATTCAAAGAA ACCTGCAGAT
    GACCAAGACC CCATTGATGC TCTCTCAGGA GATCTGGACA GCTGTCCCTC CACTACAGAA
    ACCTCACAGA ACACAGCAAA GGATAAGTGC AAGAAGGCTG CTTCCAGCTC CAAAGCACCT
    AAGAATGGAG GTAAAGCGAA GGATTCAGCA AAGACAACAG AGGAAACTTC CAAGCCAAAA
    GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865414.1
    CDS138..2306
    Translation

    Target ORF information:

    RefSeq Version XM_017009925.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009925.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA
    GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA
    GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT
    GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA
    TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG
    GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA
    AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA
    TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAACCCATA
    GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT
    GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG
    ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG
    AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG
    CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG
    AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT
    AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA
    GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT
    AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT
    GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG
    GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA
    GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC
    CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC
    AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG
    AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA
    TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC
    CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGCAGAG TGACAAAGAC
    CTCGATGATG CCTTGGATAA ACTCTCTGAC AGTCTAGGAC AAAGGCAGCC TGACCCAGAT
    GAGAACAAAC CAATGGAAGA TAAAGTAAAG GAAAAAGCTA AAGCTGAACA TAGAGACAAG
    CTTGGAGAAA GAGATGACAC TATCCCACCT GAATACAGAC ATCTCCTGGA TGATAATGGA
    CAGGACAAAC CAGTGAAGCC ACCTACAAAG AAATCAGAGG ATTCAAAGAA ACCTGCAGAT
    GACCAAGACC CCATTGATGC TCTCTCAGGA GATCTGGACA GCTGTCCCTC CACTACAGAA
    ACCTCACAGA ACACAGCAAA GGATAAGTGC AAGAAGGCTG CTTCCAGCTC CAAAGCACCT
    AAGAATGGAG GTAAAGCGAA GGATTCAGCA AAGACAACAG AGGAAACTTC CAAGCCAAAA
    GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96122
    Clone ID Related Accession (Same CDS sequence) XM_017009926.1
    Accession Version XM_017009926.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA
    GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA
    GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT
    GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA
    TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG
    GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA
    AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA
    TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAACCCATA
    GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT
    GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG
    ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG
    AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG
    CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG
    AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT
    AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA
    GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT
    AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT
    GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG
    GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA
    GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC
    CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC
    AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG
    AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA
    TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC
    CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGAGTGA CAAAGACCTC
    GATGATGCCT TGGATAAACT CTCTGACAGT CTAGGACAAA GGCAGCCTGA CCCAGATGAG
    AACAAACCAA TGGAAGATAA AGTAAAGGAA AAAGCTAAAG CTGAACATAG AGACAAGCTT
    GGAGAAAGAG ATGACACTAT CCCACCTGAA TACAGACATC TCCTGGATGA TAATGGACAG
    GACAAACCAG TGAAGCCACC TACAAAGAAA TCAGAGGATT CAAAGAAACC TGCAGATGAC
    CAAGACCCCA TTGATGCTCT CTCAGGAGAT CTGGACAGCT GTCCCTCCAC TACAGAAACC
    TCACAGAACA CAGCAAAGGA TAAGTGCAAG AAGGCTGCTT CCAGCTCCAA AGCACCTAAG
    AATGGAGGTA AAGCGAAGGA TTCAGCAAAG ACAACAGAGG AAACTTCCAA GCCAAAAGAT
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865415.1
    CDS138..2303
    Translation

    Target ORF information:

    RefSeq Version XM_017009926.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
    TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT
    CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT
    GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA
    CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA
    GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA
    GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT
    GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA
    TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG
    GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA
    AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA
    TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAACCCATA
    GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT
    GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG
    ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG
    AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG
    CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG
    AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT
    AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA
    GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT
    AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT
    GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG
    GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA
    GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC
    CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC
    AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG
    AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA
    TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC
    CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGAGTGA CAAAGACCTC
    GATGATGCCT TGGATAAACT CTCTGACAGT CTAGGACAAA GGCAGCCTGA CCCAGATGAG
    AACAAACCAA TGGAAGATAA AGTAAAGGAA AAAGCTAAAG CTGAACATAG AGACAAGCTT
    GGAGAAAGAG ATGACACTAT CCCACCTGAA TACAGACATC TCCTGGATGA TAATGGACAG
    GACAAACCAG TGAAGCCACC TACAAAGAAA TCAGAGGATT CAAAGAAACC TGCAGATGAC
    CAAGACCCCA TTGATGCTCT CTCAGGAGAT CTGGACAGCT GTCCCTCCAC TACAGAAACC
    TCACAGAACA CAGCAAAGGA TAAGTGCAAG AAGGCTGCTT CCAGCTCCAA AGCACCTAAG
    AATGGAGGTA AAGCGAAGGA TTCAGCAAAG ACAACAGAGG AAACTTCCAA GCCAAAAGAT
    GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96123
    Clone ID Related Accession (Same CDS sequence) XM_017009927.1
    Accession Version XM_017009927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAAAAGGAAG GGATCACAGG GCCTCCTGCA
    GACTCTTCGA AACCCATAGG GCCAGATGAT GCTATAGACG CCTTGTCATC TGACTTCACC
    TGTGGGTCGC CTACAGCTGC TGGAAAGAAA ACTGAAAAAG AGGAATCTAC AGAAGTTTTA
    AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG
    GTGGAGAAGG ATACAATGAG TGATCAAGCA CTCGAGGCTC TGTCGGCTTC ACTGGGCACC
    CGGCAAGCAG AACCTGAGCT CGACCTCCGC TCAATTAAGG AAGTCGATGA GGCAAAAGCT
    AAAGAAGAAA AACTAGAGAA GTGTGGTGAG GATGATGAAA CAATCCCATC TGAGTACAGA
    TTAAAACCAG CCACGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAAAACCC
    AAGCCTCGGA GTGAATCAGA ACTCATTGAT GAACTTTCAG AAGATTTTGA CCGGTCTGAA
    TGTAAAGAGA AACCATCTAA GCCAACTGAA AAGACAGAAG AATCTAAGGC CGCTGCTCCA
    GCTCCTGTGT CGGAGGCTGT GTGTCGGACC TCCATGTGTA GTATACAGTC AGCACCCCCT
    GAGCCGGCTA CCTTGAAGGG CACAGTGCCA GATGATGCTG TAGAAGCCTT GGCTGATAGC
    CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT GGAAAACCTG TGATGGATAA AGTCAAGGAG
    AAGGCCAAAG AAGAAGACCG TGAAAAGCTT GGTGAAAAAG AAGAAACAAT TCCTCCTGAT
    TATAGATTAG AAGAGGTCAA GGATAAAGAT GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG
    GAACAGCTTC CACCCATGAG TGAAGACTTC CTTCTGGATG CTTTGTCTGA GGACTTCTCT
    GGTCCACAAA ATGCTTCATC TCTTAAATTT GAAGATGCTA AACTTGCTGC TGCCATCTCT
    GAAGTGGTTT CCCAAACCCC AGCTTCAACG ACCCAAGCTG GAGCCCCACC CCGTGATACC
    TCGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG
    CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT
    GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC
    CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA
    AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT
    CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC
    AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA
    ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865416.1
    CDS169..2292
    Translation

    Target ORF information:

    RefSeq Version XM_017009927.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAAAAGGAAG GGATCACAGG GCCTCCTGCA
    GACTCTTCGA AACCCATAGG GCCAGATGAT GCTATAGACG CCTTGTCATC TGACTTCACC
    TGTGGGTCGC CTACAGCTGC TGGAAAGAAA ACTGAAAAAG AGGAATCTAC AGAAGTTTTA
    AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG
    GTGGAGAAGG ATACAATGAG TGATCAAGCA CTCGAGGCTC TGTCGGCTTC ACTGGGCACC
    CGGCAAGCAG AACCTGAGCT CGACCTCCGC TCAATTAAGG AAGTCGATGA GGCAAAAGCT
    AAAGAAGAAA AACTAGAGAA GTGTGGTGAG GATGATGAAA CAATCCCATC TGAGTACAGA
    TTAAAACCAG CCACGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAAAACCC
    AAGCCTCGGA GTGAATCAGA ACTCATTGAT GAACTTTCAG AAGATTTTGA CCGGTCTGAA
    TGTAAAGAGA AACCATCTAA GCCAACTGAA AAGACAGAAG AATCTAAGGC CGCTGCTCCA
    GCTCCTGTGT CGGAGGCTGT GTGTCGGACC TCCATGTGTA GTATACAGTC AGCACCCCCT
    GAGCCGGCTA CCTTGAAGGG CACAGTGCCA GATGATGCTG TAGAAGCCTT GGCTGATAGC
    CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT GGAAAACCTG TGATGGATAA AGTCAAGGAG
    AAGGCCAAAG AAGAAGACCG TGAAAAGCTT GGTGAAAAAG AAGAAACAAT TCCTCCTGAT
    TATAGATTAG AAGAGGTCAA GGATAAAGAT GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG
    GAACAGCTTC CACCCATGAG TGAAGACTTC CTTCTGGATG CTTTGTCTGA GGACTTCTCT
    GGTCCACAAA ATGCTTCATC TCTTAAATTT GAAGATGCTA AACTTGCTGC TGCCATCTCT
    GAAGTGGTTT CCCAAACCCC AGCTTCAACG ACCCAAGCTG GAGCCCCACC CCGTGATACC
    TCGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG
    CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT
    GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC
    CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA
    AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT
    CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC
    AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA
    ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96124
    Clone ID Related Accession (Same CDS sequence) XM_017009928.1
    Accession Version XM_017009928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC
    TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA
    GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC
    CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT
    ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC
    TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT
    AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT
    TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865417.1
    CDS207..2291
    Translation

    Target ORF information:

    RefSeq Version XM_017009928.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
    CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG
    TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC
    ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT
    AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG
    ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA
    TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT
    GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA
    ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA
    GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA
    ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC
    GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA
    GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA
    CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT
    CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG
    GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA
    ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG
    CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA
    GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA
    GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT
    AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT
    GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT
    GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA
    GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA
    CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT
    GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT
    AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT
    GGAGCCCCAC CCCGTGATAC CTCGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC
    TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA
    GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC
    CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT
    ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC
    TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT
    AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT
    TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96125
    Clone ID Related Accession (Same CDS sequence) XM_017009931.1
    Accession Version XM_017009931.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAACCC ATAGGGCCAG ATGATGCTAT AGACGCCTTG
    TCATCTGACT TCACCTGTGG GTCGCCTACA GCTGCTGGAA AGAAAACTGA AAAAGAGGAA
    TCTACAGAAG TTTTAAAAGC TCAGTCAGCA GGGACAGTCA GAAGTGCTGC TCCACCCCAA
    GAGAAGAAAA GAAAGGTGGA GAAGGATACA ATGAGTGATC AAGCACTCGA GGCTCTGTCG
    GCTTCACTGG GCACCCGGCA AGCAGAACCT GAGCTCGACC TCCGCTCAAT TAAGGAAGTC
    GATGAGGCAA AAGCTAAAGA AGAAAAACTA GAGAAGTGTG GTGAGGATGA TGAAACAATC
    CCATCTGAGT ACAGATTAAA ACCAGCCACG GATAAAGATG GAAAACCACT ATTGCCAGAG
    CCTGAAGAAA AACCCAAGCC TCGGAGTGAA TCAGAACTCA TTGATGAACT TTCAGAAGAT
    TTTGACCGGT CTGAATGTAA AGAGAAACCA TCTAAGCCAA CTGAAAAGAC AGAAGAATCT
    AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG GCTGTGTGTC GGACCTCCAT GTGTAGTATA
    CAGTCAGCAC CCCCTGAGCC GGCTACCTTG AAGGGCACAG TGCCAGATGA TGCTGTAGAA
    GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA GCAGATCCAG AAGATGGAAA ACCTGTGATG
    GATAAAGTCA AGGAGAAGGC CAAAGAAGAA GACCGTGAAA AGCTTGGTGA AAAAGAAGAA
    ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA GCCACTCCTG
    CCAAAAGAGT CTAAGGAACA GCTTCCACCC ATGAGTGAAG ACTTCCTTCT GGATGCTTTG
    TCTGAGGACT TCTCTGGTCC ACAAAATGCT TCATCTCTTA AATTTGAAGA TGCTAAACTT
    GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTT CAACGACCCA AGCTGGAGCC
    CCACCCCGTG ATACCTCGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC
    AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG
    GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT
    GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG
    AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA
    GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC
    AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA
    AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865420.1
    CDS153..2171
    Translation

    Target ORF information:

    RefSeq Version XM_017009931.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009931.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
    AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG
    CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA
    GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA
    CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC
    AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT
    AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT
    ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT
    TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT
    CCAAAATATA GGGAACTATT GGCTAAACCC ATAGGGCCAG ATGATGCTAT AGACGCCTTG
    TCATCTGACT TCACCTGTGG GTCGCCTACA GCTGCTGGAA AGAAAACTGA AAAAGAGGAA
    TCTACAGAAG TTTTAAAAGC TCAGTCAGCA GGGACAGTCA GAAGTGCTGC TCCACCCCAA
    GAGAAGAAAA GAAAGGTGGA GAAGGATACA ATGAGTGATC AAGCACTCGA GGCTCTGTCG
    GCTTCACTGG GCACCCGGCA AGCAGAACCT GAGCTCGACC TCCGCTCAAT TAAGGAAGTC
    GATGAGGCAA AAGCTAAAGA AGAAAAACTA GAGAAGTGTG GTGAGGATGA TGAAACAATC
    CCATCTGAGT ACAGATTAAA ACCAGCCACG GATAAAGATG GAAAACCACT ATTGCCAGAG
    CCTGAAGAAA AACCCAAGCC TCGGAGTGAA TCAGAACTCA TTGATGAACT TTCAGAAGAT
    TTTGACCGGT CTGAATGTAA AGAGAAACCA TCTAAGCCAA CTGAAAAGAC AGAAGAATCT
    AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG GCTGTGTGTC GGACCTCCAT GTGTAGTATA
    CAGTCAGCAC CCCCTGAGCC GGCTACCTTG AAGGGCACAG TGCCAGATGA TGCTGTAGAA
    GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA GCAGATCCAG AAGATGGAAA ACCTGTGATG
    GATAAAGTCA AGGAGAAGGC CAAAGAAGAA GACCGTGAAA AGCTTGGTGA AAAAGAAGAA
    ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA GCCACTCCTG
    CCAAAAGAGT CTAAGGAACA GCTTCCACCC ATGAGTGAAG ACTTCCTTCT GGATGCTTTG
    TCTGAGGACT TCTCTGGTCC ACAAAATGCT TCATCTCTTA AATTTGAAGA TGCTAAACTT
    GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTT CAACGACCCA AGCTGGAGCC
    CCACCCCGTG ATACCTCGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC
    AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG
    GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT
    GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG
    AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA
    GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC
    AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA
    AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102305
    Clone ID Related Accession (Same CDS sequence) NM_001330626.2 , NM_001330626.1
    Accession Version NM_001330626.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product calpastatin isoform p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001330626.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.3952.1, SRR1660805.145931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT
    GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG
    AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA
    AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA
    GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC
    CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT
    GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA
    AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT
    GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT
    GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG
    ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG
    CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA
    GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG
    CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA
    GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC
    TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA
    TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA
    ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT
    GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA
    CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA
    AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA
    CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC
    CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG
    AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA
    GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317555.1
    CDS48..2327
    Translation

    Target ORF information:

    RefSeq Version NM_001330626.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330626.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT
    GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG
    AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA
    AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA
    GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC
    CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT
    GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA
    AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT
    GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT
    GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG
    ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG
    CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA
    GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG
    CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA
    GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC
    TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA
    TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA
    ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT
    GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA
    CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA
    AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA
    CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC
    CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG
    AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA
    GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102305
    Clone ID Related Accession (Same CDS sequence) NM_001330626.2 , NM_001330626.1
    Accession Version NM_001330626.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product calpastatin isoform p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009918.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.3952.1, SRR1660805.145931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT
    GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG
    AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA
    AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA
    GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC
    CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT
    GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA
    AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT
    GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT
    GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG
    ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG
    CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA
    GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG
    CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA
    GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC
    TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA
    TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA
    ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT
    GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA
    CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA
    AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA
    CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC
    CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG
    AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA
    GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317555.1
    CDS267..2546
    Translation

    Target ORF information:

    RefSeq Version NM_001330626.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT
    GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG
    AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA
    AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA
    GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC
    CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT
    GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA
    AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT
    GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT
    GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG
    ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG
    CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA
    GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG
    CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA
    GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC
    TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA
    TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA
    ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT
    GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA
    CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA
    AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA
    CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC
    CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG
    AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA
    GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39726
    Clone ID Related Accession (Same CDS sequence) NM_001750.6 , XM_006714696.3 , NM_001750.7
    Accession Version NM_001750.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product calpastatin isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced NM_001750.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.113174.1, SRR1660803.78729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001741.4
    CDS267..2642
    Translation

    Target ORF information:

    RefSeq Version NM_001750.6
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001750.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39726
    Clone ID Related Accession (Same CDS sequence) NM_001750.6 , XM_006714696.3 , NM_001750.7
    Accession Version NM_001750.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Homo sapiens(human)
    Product calpastatin isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001750.6. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.113174.1, SRR1660803.78729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001741.4
    CDS48..2423
    Translation

    Target ORF information:

    RefSeq Version NM_001750.7
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001750.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39730
    Clone ID Related Accession (Same CDS sequence) NM_001042441.3 , XM_017009913.1 , NM_001042441.2
    Accession Version NM_001042441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product calpastatin isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001042441.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.137982.1, SRR1660805.114181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA
    GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA
    TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT
    ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG
    AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT
    TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT
    GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA
    TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT
    GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT
    GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG
    GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG
    TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC
    TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT
    AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA
    ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA
    AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT
    GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT
    GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA
    CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC
    AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG
    GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT
    GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG
    AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA
    GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC
    AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA
    AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035906.1
    CDS48..2366
    Translation

    Target ORF information:

    RefSeq Version NM_001042441.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA
    GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA
    TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT
    ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG
    AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT
    TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT
    GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA
    TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT
    GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT
    GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG
    GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG
    TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC
    TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT
    AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA
    ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA
    AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT
    GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT
    GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA
    CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC
    AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG
    GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT
    GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG
    AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA
    GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC
    AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA
    AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39730
    Clone ID Related Accession (Same CDS sequence) NM_001042441.3 , XM_017009913.1 , NM_001042441.2
    Accession Version NM_001042441.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product calpastatin isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714700.3, NM_001042441.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.114181.1, SRR1803612.137982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA
    GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA
    TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT
    ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG
    AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT
    TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT
    GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA
    TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT
    GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT
    GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG
    GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG
    TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC
    TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT
    AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA
    ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA
    AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT
    GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT
    GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA
    CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC
    AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG
    GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT
    GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG
    AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA
    GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC
    AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA
    AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035906.1
    CDS267..2585
    Translation

    Target ORF information:

    RefSeq Version NM_001042441.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA
    GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA
    TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT
    ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG
    AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT
    TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT
    GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA
    TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT
    GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT
    GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG
    GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG
    TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC
    TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT
    AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA
    ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA
    AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT
    GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT
    GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA
    CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC
    AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG
    GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT
    GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG
    AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA
    GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC
    AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA
    AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39731
    Clone ID Related Accession (Same CDS sequence) NM_001042442.3 , XM_006714701.3 , NM_001042442.2
    Accession Version NM_001042442.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Homo sapiens(human)
    Product calpastatin isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 10, 2016 this sequence version replaced NM_001329966.1, NM_001042442.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.163156.1, SRR1660807.241791.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035907.1
    CDS201..2510
    Translation

    Target ORF information:

    RefSeq Version NM_001042442.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39735
    Clone ID Related Accession (Same CDS sequence) NM_001330627.1 , NM_001330627.2
    Accession Version NM_001330627.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product calpastatin isoform q
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_006714705.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.75185.1, SRR1660807.111876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317556.1
    CDS267..2516
    Translation

    Target ORF information:

    RefSeq Version NM_001330627.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39735
    Clone ID Related Accession (Same CDS sequence) NM_001330627.1 , NM_001330627.2
    Accession Version NM_001330627.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product calpastatin isoform q
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001330627.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.201569.1, SRR1803616.75185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317556.1
    CDS48..2297
    Translation

    Target ORF information:

    RefSeq Version NM_001330627.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant 18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330627.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT
    CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC
    TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA
    GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA
    AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG
    GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA
    AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG
    TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39726
    Clone ID Related Accession (Same CDS sequence) NM_001750.6 , XM_006714696.3 , NM_001750.7
    Accession Version XM_006714696.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714759.1
    CDS158..2533
    Translation

    Target ORF information:

    RefSeq Version XM_006714696.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714696.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39727
    Clone ID Related Accession (Same CDS sequence) XM_006714697.3
    Accession Version XM_006714697.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714760.1
    CDS175..2547
    Translation

    Target ORF information:

    RefSeq Version XM_006714697.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714697.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT
    GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39728
    Clone ID Related Accession (Same CDS sequence) XM_006714698.3
    Accession Version XM_006714698.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT
    GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA
    ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT
    GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA
    CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC
    TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG
    GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA
    CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT
    GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA
    AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC
    TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA
    GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT
    GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT
    GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT
    GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC
    CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT
    GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG
    ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGCAGAGTG ACAAAGACCT CGATGATGCC
    TTGGATAAAC TCTCTGACAG TCTAGGACAA AGGCAGCCTG ACCCAGATGA GAACAAACCA
    ATGGAAGATA AAGTAAAGGA AAAAGCTAAA GCTGAACATA GAGACAAGCT TGGAGAAAGA
    GATGACACTA TCCCACCTGA ATACAGACAT CTCCTGGATG ATAATGGACA GGACAAACCA
    GTGAAGCCAC CTACAAAGAA ATCAGAGGAT TCAAAGAAAC CTGCAGATGA CCAAGACCCC
    ATTGATGCTC TCTCAGGAGA TCTGGACAGC TGTCCCTCCA CTACAGAAAC CTCACAGAAC
    ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT TCCAGCTCCA AAGCACCTAA GAATGGAGGT
    AAAGCGAAGG ATTCAGCAAA GACAACAGAG GAAACTTCCA AGCCAAAAGA TGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714761.1
    CDS175..2511
    Translation

    Target ORF information:

    RefSeq Version XM_006714698.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714698.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT
    GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA
    ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT
    GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA
    CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC
    TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG
    GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA
    CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT
    GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA
    AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC
    TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA
    GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT
    GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT
    GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT
    GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC
    CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT
    GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG
    ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGCAGAGTG ACAAAGACCT CGATGATGCC
    TTGGATAAAC TCTCTGACAG TCTAGGACAA AGGCAGCCTG ACCCAGATGA GAACAAACCA
    ATGGAAGATA AAGTAAAGGA AAAAGCTAAA GCTGAACATA GAGACAAGCT TGGAGAAAGA
    GATGACACTA TCCCACCTGA ATACAGACAT CTCCTGGATG ATAATGGACA GGACAAACCA
    GTGAAGCCAC CTACAAAGAA ATCAGAGGAT TCAAAGAAAC CTGCAGATGA CCAAGACCCC
    ATTGATGCTC TCTCAGGAGA TCTGGACAGC TGTCCCTCCA CTACAGAAAC CTCACAGAAC
    ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT TCCAGCTCCA AAGCACCTAA GAATGGAGGT
    AAAGCGAAGG ATTCAGCAAA GACAACAGAG GAAACTTCCA AGCCAAAAGA TGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39730
    Clone ID Related Accession (Same CDS sequence) NM_001042441.3 , XM_017009913.1 , NM_001042441.2
    Accession Version XM_017009913.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA
    GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA
    TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT
    ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG
    AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT
    TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT
    GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA
    TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT
    GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT
    GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG
    GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG
    TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC
    TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT
    AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA
    ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA
    AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT
    GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT
    GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA
    CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC
    AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG
    GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT
    GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG
    AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA
    GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC
    AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA
    AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865402.1
    CDS173..2491
    Translation

    Target ORF information:

    RefSeq Version XM_017009913.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009913.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA
    GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA
    TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT
    ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG
    AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT
    TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT
    GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA
    TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT
    GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT
    GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG
    GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG
    TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC
    TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT
    AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA
    ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA
    AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT
    GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT
    GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA
    CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC
    AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG
    GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT
    GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG
    AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA
    GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC
    AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA
    AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39731
    Clone ID Related Accession (Same CDS sequence) NM_001042442.3 , XM_006714701.3 , NM_001042442.2
    Accession Version XM_006714701.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714764.1
    CDS200..2509
    Translation

    Target ORF information:

    RefSeq Version XM_006714701.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714701.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA
    CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT
    AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA
    CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG
    GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC
    AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT
    GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA
    GAGGAAACTT CCAAGCCAAA AGATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39733
    Clone ID Related Accession (Same CDS sequence) XM_006714703.3
    Accession Version XM_006714703.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAACCCA TAGGGCCAGA TGATGCTATA
    GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA
    AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT
    CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG
    GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT
    AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT
    GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA
    TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT
    TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA
    GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG
    TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT
    GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA
    CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA
    AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG
    CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG
    GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT
    GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA
    GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714766.1
    CDS200..2470
    Translation

    Target ORF information:

    RefSeq Version XM_006714703.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714703.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAACCCA TAGGGCCAGA TGATGCTATA
    GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA
    AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT
    CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG
    GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT
    AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT
    GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA
    TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT
    TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA
    GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG
    TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT
    GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA
    CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA
    AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG
    CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG
    GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT
    GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA
    GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56642
    Clone ID Related Accession (Same CDS sequence) XM_011543654.2
    Accession Version XM_011543654.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA AAAAAGAAAA GAAATCATTA ACCCCAGCTG TGCCAGTTGA ATCTAAACCG
    GATAAACCAT CGGGAAAGTC AGGCATGGAT GCTGCTTTGG ATGACTTAAT AGATACTTTA
    GGAGGACCTG AAGAAACTGA AGAAGAAAAT ACAACGTATA CTGGACCAGA AGTTTCAGAT
    CCAATGAGTT CCACCTACAT AGAGGAATTG GGTAAAAGAG AAGTCACAAT TCCTCCAAAA
    TATAGGGAAC TATTGGCTAA AAAGGAAGGG ATCACAGGGC CTCCTGCAGA CTCTTCGAAA
    CCCATAGGGC CAGATGATGC TATAGACGCC TTGTCATCTG ACTTCACCTG TGGGTCGCCT
    ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG GAATCTACAG AAGTTTTAAA AGCTCAGTCA
    GCAGGGACAG TCAGAAGTGC TGCTCCACCC CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT
    ACAATGAGTG ATCAAGCACT CGAGGCTCTG TCGGCTTCAC TGGGCACCCG GCAAGCAGAA
    CCTGAGCTCG ACCTCCGCTC AATTAAGGAA GTCGATGAGG CAAAAGCTAA AGAAGAAAAA
    CTAGAGAAGT GTGGTGAGGA TGATGAAACA ATCCCATCTG AGTACAGATT AAAACCAGCC
    ACGGATAAAG ATGGAAAACC ACTATTGCCA GAGCCTGAAG AAAAACCCAA GCCTCGGAGT
    GAATCAGAAC TCATTGATGA ACTTTCAGAA GATTTTGACC GGTCTGAATG TAAAGAGAAA
    CCATCTAAGC CAACTGAAAA GACAGAAGAA TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG
    GAGGCTGTGT GTCGGACCTC CATGTGTAGT ATACAGTCAG CACCCCCTGA GCCGGCTACC
    TTGAAGGGCA CAGTGCCAGA TGATGCTGTA GAAGCCTTGG CTGATAGCCT GGGGAAAAAG
    GAAGCAGATC CAGAAGATGG AAAACCTGTG ATGGATAAAG TCAAGGAGAA GGCCAAAGAA
    GAAGACCGTG AAAAGCTTGG TGAAAAAGAA GAAACAATTC CTCCTGATTA TAGATTAGAA
    GAGGTCAAGG ATAAAGATGG AAAGCCACTC CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA
    CCCATGAGTG AAGACTTCCT TCTGGATGCT TTGTCTGAGG ACTTCTCTGG TCCACAAAAT
    GCTTCATCTC TTAAATTTGA AGATGCTAAA CTTGCTGCTG CCATCTCTGA AGTGGTTTCC
    CAAACCCCAG CTTCAACGAC CCAAGCTGGA GCCCCACCCC GTGATACCTC GCAGAGTGAC
    AAAGACCTCG ATGATGCCTT GGATAAACTC TCTGACAGTC TAGGACAAAG GCAGCCTGAC
    CCAGATGAGA ACAAACCAAT GGAAGATAAA GTAAAGGAAA AAGCTAAAGC TGAACATAGA
    GACAAGCTTG GAGAAAGAGA TGACACTATC CCACCTGAAT ACAGACATCT CCTGGATGAT
    AATGGACAGG ACAAACCAGT GAAGCCACCT ACAAAGAAAT CAGAGGATTC AAAGAAACCT
    GCAGATGACC AAGACCCCAT TGATGCTCTC TCAGGAGATC TGGACAGCTG TCCCTCCACT
    ACAGAAACCT CACAGAACAC AGCAAAGGAT AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA
    GCACCTAAGA ATGGAGGTAA AGCGAAGGAT TCAGCAAAGA CAACAGAGGA AACTTCCAAG
    CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541956.1
    CDS175..2469
    Translation

    Target ORF information:

    RefSeq Version XM_011543654.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011543654.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA AAAAAGAAAA GAAATCATTA ACCCCAGCTG TGCCAGTTGA ATCTAAACCG
    GATAAACCAT CGGGAAAGTC AGGCATGGAT GCTGCTTTGG ATGACTTAAT AGATACTTTA
    GGAGGACCTG AAGAAACTGA AGAAGAAAAT ACAACGTATA CTGGACCAGA AGTTTCAGAT
    CCAATGAGTT CCACCTACAT AGAGGAATTG GGTAAAAGAG AAGTCACAAT TCCTCCAAAA
    TATAGGGAAC TATTGGCTAA AAAGGAAGGG ATCACAGGGC CTCCTGCAGA CTCTTCGAAA
    CCCATAGGGC CAGATGATGC TATAGACGCC TTGTCATCTG ACTTCACCTG TGGGTCGCCT
    ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG GAATCTACAG AAGTTTTAAA AGCTCAGTCA
    GCAGGGACAG TCAGAAGTGC TGCTCCACCC CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT
    ACAATGAGTG ATCAAGCACT CGAGGCTCTG TCGGCTTCAC TGGGCACCCG GCAAGCAGAA
    CCTGAGCTCG ACCTCCGCTC AATTAAGGAA GTCGATGAGG CAAAAGCTAA AGAAGAAAAA
    CTAGAGAAGT GTGGTGAGGA TGATGAAACA ATCCCATCTG AGTACAGATT AAAACCAGCC
    ACGGATAAAG ATGGAAAACC ACTATTGCCA GAGCCTGAAG AAAAACCCAA GCCTCGGAGT
    GAATCAGAAC TCATTGATGA ACTTTCAGAA GATTTTGACC GGTCTGAATG TAAAGAGAAA
    CCATCTAAGC CAACTGAAAA GACAGAAGAA TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG
    GAGGCTGTGT GTCGGACCTC CATGTGTAGT ATACAGTCAG CACCCCCTGA GCCGGCTACC
    TTGAAGGGCA CAGTGCCAGA TGATGCTGTA GAAGCCTTGG CTGATAGCCT GGGGAAAAAG
    GAAGCAGATC CAGAAGATGG AAAACCTGTG ATGGATAAAG TCAAGGAGAA GGCCAAAGAA
    GAAGACCGTG AAAAGCTTGG TGAAAAAGAA GAAACAATTC CTCCTGATTA TAGATTAGAA
    GAGGTCAAGG ATAAAGATGG AAAGCCACTC CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA
    CCCATGAGTG AAGACTTCCT TCTGGATGCT TTGTCTGAGG ACTTCTCTGG TCCACAAAAT
    GCTTCATCTC TTAAATTTGA AGATGCTAAA CTTGCTGCTG CCATCTCTGA AGTGGTTTCC
    CAAACCCCAG CTTCAACGAC CCAAGCTGGA GCCCCACCCC GTGATACCTC GCAGAGTGAC
    AAAGACCTCG ATGATGCCTT GGATAAACTC TCTGACAGTC TAGGACAAAG GCAGCCTGAC
    CCAGATGAGA ACAAACCAAT GGAAGATAAA GTAAAGGAAA AAGCTAAAGC TGAACATAGA
    GACAAGCTTG GAGAAAGAGA TGACACTATC CCACCTGAAT ACAGACATCT CCTGGATGAT
    AATGGACAGG ACAAACCAGT GAAGCCACCT ACAAAGAAAT CAGAGGATTC AAAGAAACCT
    GCAGATGACC AAGACCCCAT TGATGCTCTC TCAGGAGATC TGGACAGCTG TCCCTCCACT
    ACAGAAACCT CACAGAACAC AGCAAAGGAT AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA
    GCACCTAAGA ATGGAGGTAA AGCGAAGGAT TCAGCAAAGA CAACAGAGGA AACTTCCAAG
    CCAAAAGATG ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56643
    Clone ID Related Accession (Same CDS sequence) XM_011543655.2
    Accession Version XM_011543655.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA
    TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA
    TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG
    GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG
    AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA
    GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA
    GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG
    GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC
    ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT
    TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA
    ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA
    GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA
    GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC
    AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT
    GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA
    GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA
    GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA
    CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA
    AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541957.1
    CDS175..2466
    Translation

    Target ORF information:

    RefSeq Version XM_011543655.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011543655.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG
    CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT
    GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA
    GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG
    AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA
    CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG
    GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA
    TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA
    TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG
    GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG
    AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA
    GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA
    GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG
    GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC
    ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT
    TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA
    ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA
    GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA
    GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC
    AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT
    GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA
    GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA
    GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA
    CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA
    AAAGATGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56644
    Clone ID Related Accession (Same CDS sequence) XM_011543656.2
    Accession Version XM_011543656.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGAAAAAAG AAAAGAAATC ATTAACCCCA GCTGTGCCAG TTGAATCTAA ACCGGATAAA
    CCATCGGGAA AGTCAGGCAT GGATGCTGCT TTGGATGACT TAATAGATAC TTTAGGAGGA
    CCTGAAGAAA CTGAAGAAGA AAATACAACG TATACTGGAC CAGAAGTTTC AGATCCAATG
    AGTTCCACCT ACATAGAGGA ATTGGGTAAA AGAGAAGTCA CAATTCCTCC AAAATATAGG
    GAACTATTGG CTAAAAAGGA AGGGATCACA GGGCCTCCTG CAGACTCTTC GAAACCCATA
    GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT
    GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG
    ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG
    AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG
    CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG
    AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT
    AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA
    GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT
    AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT
    GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG
    GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA
    GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC
    CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC
    AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG
    AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA
    TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC
    CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGCAGAG TGACAAAGAC
    CTCGATGATG CCTTGGATAA ACTCTCTGAC AGTCTAGGAC AAAGGCAGCC TGACCCAGAT
    GAGAACAAAC CAATGGAAGA TAAAGTAAAG GAAAAAGCTA AAGCTGAACA TAGAGACAAG
    CTTGGAGAAA GAGATGACAC TATCCCACCT GAATACAGAC ATCTCCTGGA TGATAATGGA
    CAGGACAAAC CAGTGAAGCC ACCTACAAAG AAATCAGAGG ATTCAAAGAA ACCTGCAGAT
    GACCAAGACC CCATTGATGC TCTCTCAGGA GATCTGGACA GCTGTCCCTC CACTACAGAA
    ACCTCACAGA ACACAGCAAA GGATAAGTGC AAGAAGGCTG CTTCCAGCTC CAAAGCACCT
    AAGAATGGAG GTAAAGCGAA GGATTCAGCA AAGACAACAG AGGAAACTTC CAAGCCAAAA
    GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541958.1
    CDS185..2413
    Translation

    Target ORF information:

    RefSeq Version XM_011543656.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011543656.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGAAAAAAG AAAAGAAATC ATTAACCCCA GCTGTGCCAG TTGAATCTAA ACCGGATAAA
    CCATCGGGAA AGTCAGGCAT GGATGCTGCT TTGGATGACT TAATAGATAC TTTAGGAGGA
    CCTGAAGAAA CTGAAGAAGA AAATACAACG TATACTGGAC CAGAAGTTTC AGATCCAATG
    AGTTCCACCT ACATAGAGGA ATTGGGTAAA AGAGAAGTCA CAATTCCTCC AAAATATAGG
    GAACTATTGG CTAAAAAGGA AGGGATCACA GGGCCTCCTG CAGACTCTTC GAAACCCATA
    GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT
    GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG
    ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG
    AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG
    CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG
    AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT
    AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA
    GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT
    AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT
    GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG
    GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA
    GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC
    CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC
    AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG
    AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA
    TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC
    CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGCAGAG TGACAAAGAC
    CTCGATGATG CCTTGGATAA ACTCTCTGAC AGTCTAGGAC AAAGGCAGCC TGACCCAGAT
    GAGAACAAAC CAATGGAAGA TAAAGTAAAG GAAAAAGCTA AAGCTGAACA TAGAGACAAG
    CTTGGAGAAA GAGATGACAC TATCCCACCT GAATACAGAC ATCTCCTGGA TGATAATGGA
    CAGGACAAAC CAGTGAAGCC ACCTACAAAG AAATCAGAGG ATTCAAAGAA ACCTGCAGAT
    GACCAAGACC CCATTGATGC TCTCTCAGGA GATCTGGACA GCTGTCCCTC CACTACAGAA
    ACCTCACAGA ACACAGCAAA GGATAAGTGC AAGAAGGCTG CTTCCAGCTC CAAAGCACCT
    AAGAATGGAG GTAAAGCGAA GGATTCAGCA AAGACAACAG AGGAAACTTC CAAGCCAAAA
    GATGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96112
    Clone ID Related Accession (Same CDS sequence) XM_017009911.1 , XM_017009912.1
    Accession Version XM_017009911.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT
    GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA
    ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT
    GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA
    CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC
    TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG
    GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA
    CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT
    GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA
    AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC
    TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA
    GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT
    GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT
    GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT
    GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC
    CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT
    GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG
    ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGAGTGACA AAGACCTCGA TGATGCCTTG
    GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG
    GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT
    GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG
    AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT
    GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA
    GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA
    GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865400.1
    CDS175..2508
    Translation

    Target ORF information:

    RefSeq Version XM_017009911.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT
    GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA
    ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT
    GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA
    CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC
    TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG
    GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA
    CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT
    GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA
    AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC
    TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA
    GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT
    GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT
    GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT
    GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC
    CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT
    GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG
    ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGAGTGACA AAGACCTCGA TGATGCCTTG
    GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG
    GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT
    GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG
    AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT
    GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA
    GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA
    GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96112
    Clone ID Related Accession (Same CDS sequence) XM_017009911.1 , XM_017009912.1
    Accession Version XM_017009912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2334bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT
    GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA
    ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT
    GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA
    CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC
    TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG
    GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA
    CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT
    GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA
    AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC
    TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA
    GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT
    GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT
    GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT
    GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC
    CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT
    GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG
    ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGAGTGACA AAGACCTCGA TGATGCCTTG
    GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG
    GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT
    GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG
    AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT
    GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA
    GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA
    GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865401.1
    CDS186..2519
    Translation

    Target ORF information:

    RefSeq Version XM_017009912.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT
    GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA
    ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT
    GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA
    CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC
    TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG
    GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA
    CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT
    GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA
    AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC
    TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA
    GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT
    GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT
    GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT
    GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC
    CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT
    GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG
    ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGAGTGACA AAGACCTCGA TGATGCCTTG
    GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG
    GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT
    GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG
    AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT
    GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA
    GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA
    GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96113
    Clone ID Related Accession (Same CDS sequence) XM_017009914.1
    Accession Version XM_017009914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA
    GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA
    TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT
    ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG
    AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT
    TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT
    GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA
    TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT
    GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT
    GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG
    GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG
    TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC
    TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT
    AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA
    ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA
    AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT
    GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT
    GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA
    CCCCGTGATA CCTCGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865403.1
    CDS175..2490
    Translation

    Target ORF information:

    RefSeq Version XM_017009914.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA
    GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA
    TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT
    ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG
    AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT
    TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT
    GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA
    TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT
    GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT
    GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG
    GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG
    TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC
    TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT
    AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA
    ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA
    AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT
    GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT
    GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA
    CCCCGTGATA CCTCGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT
    CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA
    AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA
    TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA
    TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT
    CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG
    AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG
    ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96114
    Clone ID Related Accession (Same CDS sequence) XM_017009916.1
    Accession Version XM_017009916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGAGTG ACAAAGACCT CGATGATGCC TTGGATAAAC TCTCTGACAG TCTAGGACAA
    AGGCAGCCTG ACCCAGATGA GAACAAACCA ATGGAAGATA AAGTAAAGGA AAAAGCTAAA
    GCTGAACATA GAGACAAGCT TGGAGAAAGA GATGACACTA TCCCACCTGA ATACAGACAT
    CTCCTGGATG ATAATGGACA GGACAAACCA GTGAAGCCAC CTACAAAGAA ATCAGAGGAT
    TCAAAGAAAC CTGCAGATGA CCAAGACCCC ATTGATGCTC TCTCAGGAGA TCTGGACAGC
    TGTCCCTCCA CTACAGAAAC CTCACAGAAC ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT
    TCCAGCTCCA AAGCACCTAA GAATGGAGGT AAAGCGAAGG ATTCAGCAAA GACAACAGAG
    GAAACTTCCA AGCCAAAAGA TGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865405.1
    CDS200..2506
    Translation

    Target ORF information:

    RefSeq Version XM_017009916.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA
    CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC
    AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA
    AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA
    ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA
    GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC
    TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA
    CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC
    ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT
    GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC
    ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT
    TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA
    AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC
    ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA
    GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC
    AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA
    CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT
    GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT
    CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC
    CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT
    AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG
    GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT
    GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT
    AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC
    TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC
    TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT
    ACCTCGAGTG ACAAAGACCT CGATGATGCC TTGGATAAAC TCTCTGACAG TCTAGGACAA
    AGGCAGCCTG ACCCAGATGA GAACAAACCA ATGGAAGATA AAGTAAAGGA AAAAGCTAAA
    GCTGAACATA GAGACAAGCT TGGAGAAAGA GATGACACTA TCCCACCTGA ATACAGACAT
    CTCCTGGATG ATAATGGACA GGACAAACCA GTGAAGCCAC CTACAAAGAA ATCAGAGGAT
    TCAAAGAAAC CTGCAGATGA CCAAGACCCC ATTGATGCTC TCTCAGGAGA TCTGGACAGC
    TGTCCCTCCA CTACAGAAAC CTCACAGAAC ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT
    TCCAGCTCCA AAGCACCTAA GAATGGAGGT AAAGCGAAGG ATTCAGCAAA GACAACAGAG
    GAAACTTCCA AGCCAAAAGA TGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96115
    Clone ID Related Accession (Same CDS sequence) XM_017009919.1
    Accession Version XM_017009919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT
    GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA
    ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT
    GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA
    CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC
    TCAATTAAGG AAGTCGATGA GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865408.1
    CDS175..2427
    Translation

    Target ORF information:

    RefSeq Version XM_017009919.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA
    AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT
    GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA
    GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC
    ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT
    GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG
    GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT
    ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA
    GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT
    GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA
    ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT
    GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA
    CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC
    TCAATTAAGG AAGTCGATGA GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA
    AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG
    TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT
    GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA
    CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT
    GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC
    AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT
    CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG
    TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC
    TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC
    ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT
    GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96118
    Clone ID Related Accession (Same CDS sequence) XM_017009922.1
    Accession Version XM_017009922.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAC CCATAGGGCC AGATGATGCT
    ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT
    GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT
    GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC
    GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA
    ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT
    GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA
    CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA
    CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG
    ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC
    ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT
    GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA
    AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT
    GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA
    AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT
    CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA
    GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC
    CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA TGATGCCTTG
    GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG
    GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT
    GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG
    AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT
    GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA
    GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA
    GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865411.1
    CDS175..2388
    Translation

    Target ORF information:

    RefSeq Version XM_017009922.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009922.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAC CCATAGGGCC AGATGATGCT
    ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT
    GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT
    GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC
    GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA
    ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT
    GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA
    CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA
    CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG
    ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC
    ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT
    GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA
    AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT
    GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA
    AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT
    CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA
    GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC
    CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA TGATGCCTTG
    GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG
    GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT
    GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG
    AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT
    GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA
    GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA
    GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96119
    Clone ID Related Accession (Same CDS sequence) XM_017009923.1
    Accession Version XM_017009923.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAC CCATAGGGCC AGATGATGCT
    ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT
    GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT
    GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC
    GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA
    ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT
    GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA
    CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA
    CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG
    ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC
    ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT
    GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA
    AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT
    GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA
    AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT
    CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA
    GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC
    CAAGCTGGAG CCCCACCCCG TGATACCTCG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865412.1
    CDS175..2385
    Translation

    Target ORF information:

    RefSeq Version XM_017009923.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009923.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA
    CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT
    CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA
    CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT
    GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG
    CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT
    GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT
    GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA
    GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAC CCATAGGGCC AGATGATGCT
    ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT
    GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT
    GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC
    GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA
    ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT
    GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA
    CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA
    CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG
    ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC
    ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT
    GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA
    AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT
    GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA
    AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT
    CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA
    GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC
    CAAGCTGGAG CCCCACCCCG TGATACCTCG AGTGACAAAG ACCTCGATGA TGCCTTGGAT
    AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA
    GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC
    ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG
    CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT
    GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA
    AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG
    AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96120
    Clone ID Related Accession (Same CDS sequence) XM_017009924.1
    Accession Version XM_017009924.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product calpastatin isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT
    GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG
    AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA
    AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA
    GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC
    CGCTCAATTA AGGAAGTCGA TGAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865413.1
    CDS175..2367
    Translation

    Target ORF information:

    RefSeq Version XM_017009924.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calpastatin (CAST), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
    CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA
    AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC
    TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG
    TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA
    CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG
    AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC
    GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA
    ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT
    ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA
    GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT
    TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG
    TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA
    AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT
    GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG
    AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA
    AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA
    GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC
    CGCTCAATTA AGGAAGTCGA TGAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA
    GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC
    CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC
    GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA
    GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG
    GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA
    GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT
    CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA
    GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG
    GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT
    GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC
    CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA
    GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA
    GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC
    AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA
    GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG
    GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG
    GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA
    ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.