CAST cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAST
Entrez Gene ID	831
Full Name	calpastatin
Synonyms	BS-17,PLACK
General protein information	Preferred Names calpastatin Names calpastatin calpain inhibitor sperm BS-17 component
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q15
Summary	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010].
Disorder MIM:	114090
Disorder Html:	Peeling skin with leukonychia, acral punctate keratoses, cheilitis, and knuckle pads, 616295 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017009919.1	XP_016865408.1	calpastatin isoform X17
XM_017009923.1	XP_016865412.1	calpastatin isoform X23
XM_017009920.1	XP_016865409.1	calpastatin isoform X20
XM_006714713.3	XP_006714776.1	calpastatin isoform X42
NM_001330629.1	NP_001317558.1	calpastatin isoform s
NM_173060.3	NP_775083.1	calpastatin isoform b
NM_001042442.3	NP_001035907.1	calpastatin isoform h
NM_001330631.1	NP_001317560.1	calpastatin isoform m
NM_001330629.2	NP_001317558.1	calpastatin isoform s
NM_001750.6	NP_001741.4	calpastatin isoform a
XM_011543658.2	XP_011541960.1	calpastatin isoform X39
XM_006714714.3	XP_006714777.1	calpastatin isoform X43
XM_011543654.2	XP_011541956.1	calpastatin isoform X10
XM_006714703.3	XP_006714766.1	calpastatin isoform X15
XM_006714697.3	XP_006714760.1	calpastatin isoform X2
XM_006714701.3	XP_006714764.1	calpastatin isoform X8
XM_006714708.2	XP_006714771.1	calpastatin isoform X1
XM_017009924.1	XP_016865413.1	calpastatin isoform X25
NM_001042440.3	NP_001035905.1	calpastatin isoform f
NM_001330627.1	NP_001317556.1	calpastatin isoform q
NM_001042444.2	NP_001035909.1	calpastatin isoform j
NM_001330634.1	NP_001317563.1	calpastatin isoform w
NM_001042446.2	NP_001035911.1	calpastatin isoform l
NM_001042441.3	NP_001035906.1	calpastatin isoform g
NM_001330628.1	NP_001317557.1	calpastatin isoform r
XM_017009925.1	XP_016865414.1	calpastatin isoform X27
XM_017009926.1	XP_016865415.1	calpastatin isoform X28
XM_017009927.1	XP_016865416.1	calpastatin isoform X30
XM_006714715.3	XP_006714778.1	calpastatin isoform X44
XM_017009913.1	XP_016865402.1	calpastatin isoform X6
XM_017009916.1	XP_016865405.1	calpastatin isoform X9
XM_017009922.1	XP_016865411.1	calpastatin isoform X22
NM_173060.4	NP_775083.1	calpastatin isoform b
NM_001330627.2	NP_001317556.1	calpastatin isoform q
NM_001190442.1	NP_001177371.1	calpastatin isoform m
XM_017009921.1	XP_016865410.1	calpastatin isoform X21
XM_017009931.1	XP_016865420.1	calpastatin isoform X37
XM_006714696.3	XP_006714759.1	calpastatin isoform X1
XM_006714699.3	XP_006714762.1	calpastatin isoform X5
NM_001042440.4	NP_001035905.1	calpastatin isoform f
XM_017009928.1	XP_016865417.1	calpastatin isoform X32
NM_001330626.2	NP_001317555.1	calpastatin isoform p
NM_001330628.2	NP_001317557.1	calpastatin isoform r
NM_001042445.2	NP_001035910.1	calpastatin isoform k
NM_001042440.5	NP_001035905.1	calpastatin isoform f
NM_001330632.1	NP_001317561.1	calpastatin isoform u
NM_001330630.1	NP_001317559.1	calpastatin isoform t
NM_001750.7	NP_001741.4	calpastatin isoform a
NM_001284213.3	NP_001271142.1	calpastatin isoform o
NM_001330633.1	NP_001317562.1	calpastatin isoform v
NM_001330626.1	NP_001317555.1	calpastatin isoform p
NM_001042441.2	NP_001035906.1	calpastatin isoform g
NM_001042442.2	NP_001035907.1	calpastatin isoform h
XM_017009911.1	XP_016865400.1	calpastatin isoform X4
XM_017009912.1	XP_016865401.1	calpastatin isoform X4
XM_017009914.1	XP_016865403.1	calpastatin isoform X7
NM_001284212.1	NP_001271141.1	calpastatin isoform n
XM_011543655.2	XP_011541957.1	calpastatin isoform X12
XM_006714702.3	XP_006714765.1	calpastatin isoform X14
XM_006714704.3	XP_006714767.1	calpastatin isoform X16
XM_011543656.2	XP_011541958.1	calpastatin isoform X19
XM_006714698.3	XP_006714761.1	calpastatin isoform X3
XM_011543657.2	XP_011541959.1	calpastatin isoform X26
NM_001284212.3	NP_001271141.1	calpastatin isoform n
NM_001042443.2	NP_001035908.1	calpastatin isoform i
NM_001375317.1	NP_001362246.1	calpastatin isoform x

Pathways from BioSystems

Homologies

Homo sapiens (human)	CAST	NP_001035905.1
	CAST	XP_001091928.2
Mus musculus (house mouse)	Cast	NP_033947.1
Rattus norvegicus (Norway rat)	Cast	NP_445747.2
Gallus gallus (chicken)	CAST	NP_001131122.1
Pan troglodytes (chimpanzee)	CAST	XP_518168.4
Canis lupus familiaris (dog)	CAST	XP_005618134.1
Bos taurus (cattle)	CAST	NP_001025489.1
Xenopus tropicalis (tropical clawed frog)	cast	NP_001120309.1

Gene Ontology
Bibliography

Related articles in PubMed

KSHV reduces autophagy in THP-1 cells and in differentiating monocytes by decreasing CAST/calpastatin and ATG5 expression.
Santarelli R, Granato M, Pentassuglia G, Lacconi V, Gilardini Montani MS, Gonnella R, Tafani M, Torrisi MR, Faggioni A, Cirone M
Autophagy12(12)2311-2325(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kaposi sarcoma-associated herpesvirus reduces CAST (calpastatin) and consequently decreases ATG5 expression in both THP-1 monocytoid cells and primary monocytes.
Title: KSHV reduces autophagy in THP-1 cells and in differentiating monocytes by decreasing CAST/calpastatin and ATG5 expression.

calpains and calpastatin in patients with idiopathic pulmonary arterial hypertension (PAH) and mice with hypoxic or spontaneous (SM22-5HTT(+) strain) PH, were investigated.
Title: Extracellular Calpain/Calpastatin Balance Is Involved in the Progression of Pulmonary Hypertension.

We describe PLACK syndrome, as a clinical entity of defective epidermal adhesion, caused by loss-of-function mutations in calpastatin.
Title: Loss-of-function mutations in CAST cause peeling skin, leukonychia, acral punctate keratoses, cheilitis, and knuckle pads.

Loss of CAST in angiogenic ECs facilitates mu-calpain-induced SOCS3 degradation, which amplifies pathological angiogenesis through interleukin-6/STAT3/VEGF-C axis.
Title: Calpastatin counteracts pathological angiogenesis by inhibiting suppressor of cytokine signaling 3 degradation in vascular endothelial cells.

Calpastatin binds with Cav1.2 amino acid motifs in a calcium dependent manner.
Title: The Ca(2+)-dependent interaction of calpastatin domain L with the C-terminal tail of the Cav1.2 channel.

The following CAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16872	NM_001284212.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu20713	NM_001042440.5 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu21528	NM_173060.4 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu39740	NM_001042444.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu39744	XM_006714715.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu21399	NM_001284213.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu39741	NM_001042446.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu39742	XM_006714713.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu39743	XM_006714714.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu56646	XM_011543658.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu21220	NM_001330631.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu21220	NM_001190442.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39736	NM_001330628.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39737	NM_001042443.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39738	NM_001330633.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39739	NM_001042445.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu21220	XM_006714708.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39729	XM_006714699.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39732	XM_006714702.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39734	XM_006714704.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu56645	XM_011543657.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu102190	NM_001330630.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu102283	NM_001330632.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102294	NM_001330634.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102380	NM_001330629.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu39731	NM_001042442.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu39734	NM_001375317.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96116	XM_017009920.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96117	XM_017009921.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96121	XM_017009925.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96122	XM_017009926.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96123	XM_017009927.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96124	XM_017009928.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96125	XM_017009931.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102305	NM_001330626.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39726	NM_001750.7 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39730	NM_001042441.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39735	NM_001330627.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39726	XM_006714696.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39727	XM_006714697.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39728	XM_006714698.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39730	XM_017009913.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39731	XM_006714701.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39733	XM_006714703.3 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56642	XM_011543654.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56643	XM_011543655.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56644	XM_011543656.2 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu96112	XM_017009911.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu96112	XM_017009912.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu96113	XM_017009914.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu96114	XM_017009916.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu96115	XM_017009919.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu96118	XM_017009922.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu96119	XM_017009923.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu96120	XM_017009924.1 Latest version!	Homo sapiens calpastatin (CAST), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu102380	NM_001330629.1	Homo sapiens calpastatin (CAST), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu21528	NM_173060.3	Homo sapiens calpastatin (CAST), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu39735	NM_001330627.1	Homo sapiens calpastatin (CAST), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu20713	NM_001042440.3	Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu39736	NM_001330628.1	Homo sapiens calpastatin (CAST), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu20713	NM_001042440.4	Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu39726	NM_001750.6	Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu102305	NM_001330626.1	Homo sapiens calpastatin (CAST), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39730	NM_001042441.2	Homo sapiens calpastatin (CAST), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu39731	NM_001042442.2	Homo sapiens calpastatin (CAST), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu16872	NM_001284212.1	Homo sapiens calpastatin (CAST), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAST ( NM_001284212.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271141.1 Homo sapiens calpastatin (CAST), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu16872
Clone ID Related Accession (Same CDS sequence)	NM_001284212.1 , NM_001284212.3
Accession Version	NM_001284212.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-12
Organism	Homo sapiens(Human)
Product	calpastatin isoform n
Comment

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC 
TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT 
GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA 
GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG 
GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG 
CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA 
GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA 
AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG 
CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT 
AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT 
CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG 
CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG 
GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG 
GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT 
AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA 
CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT 
CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA 
GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001271141.1

Translation

MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001284212.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284212.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC 
TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT 
GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA 
GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG 
GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG 
CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA 
GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA 
AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG 
CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT 
AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT 
CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG 
CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG 
GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG 
GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT 
AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA 
CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT 
CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA 
GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001284212.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271141.1 Homo sapiens calpastatin (CAST), transcript variant 13, mRNA.

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CloneID	OHu16872
Clone ID Related Accession (Same CDS sequence)	NM_001284212.1 , NM_001284212.3
Accession Version	NM_001284212.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform n
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, BX648397.1, U31345.1 and AC008906.5. On Aug 30, 2016 this sequence version replaced NM_001284212.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.23360.1, SRR1803611.111838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC 
TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT 
GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA 
GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG 
GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG 
CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA 
GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA 
AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG 
CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT 
AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT 
CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG 
CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG 
GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG 
GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT 
AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA 
CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT 
CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA 
GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271141.1
CDS	296..2296
Translation	MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001284212.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284212.3

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC 
TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT 
GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA 
GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG 
GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG 
CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA 
GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA 
AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG 
CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT 
AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT 
CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG 
CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG 
GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG 
GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT 
AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA 
CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT 
CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA 
GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042440.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035905.1 Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu20713
Clone ID Related Accession (Same CDS sequence)	NM_001042440.3 , NM_001042440.4 , NM_001042440.5
Accession Version	NM_001042440.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-12
Organism	Homo sapiens(Human)
Product	calpastatin isoform f
Comment

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001035905.1

Translation

MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042440.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042440.3

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042440.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035905.1 Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu20713
Clone ID Related Accession (Same CDS sequence)	NM_001042440.3 , NM_001042440.4 , NM_001042440.5
Accession Version	NM_001042440.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	calpastatin isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced NM_001042440.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.47151.1, SRR1803613.226150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035905.1
CDS	267..2519
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042440.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042440.4

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042440.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035905.1 Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu20713
Clone ID Related Accession (Same CDS sequence)	NM_001042440.3 , NM_001042440.4 , NM_001042440.5
Accession Version	NM_001042440.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001042440.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.226150.1, SRR1803613.6171.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035905.1
CDS	48..2300
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042440.5
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042440.5

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_173060.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_775083.1 Homo sapiens calpastatin (CAST), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21528
Clone ID Related Accession (Same CDS sequence)	NM_173060.3 , NM_173060.4
Accession Version	NM_173060.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-12
Organism	Homo sapiens(Human)
Product	calpastatin isoform b
Comment

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT 
TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG 
TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT 
CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG 
AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA 
GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA 
GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA 
CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT 
CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA 
GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT 
GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG 
GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG 
AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC 
AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG 
CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA 
CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGCAG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_775083.1

Translation

MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_173060.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173060.3

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT 
TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG 
TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT 
CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG 
AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA 
GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA 
GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA 
CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT 
CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA 
GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT 
GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG 
GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG 
AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC 
AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG 
CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA 
CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGCAG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_173060.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_775083.1 Homo sapiens calpastatin (CAST), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21528
Clone ID Related Accession (Same CDS sequence)	NM_173060.3 , NM_173060.4
Accession Version	NM_173060.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AL832349.1, AL832174.1, AY189741.1 and AC008906.5. On Aug 30, 2016 this sequence version replaced NM_173060.3. Transcript Variant: This variant (2) represents use of an alternate promoter compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832349.1, SRR1803617.153476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT 
TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG 
TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT 
CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG 
AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA 
GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA 
GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA 
CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT 
CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA 
GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT 
GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG 
GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG 
AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC 
AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG 
CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA 
CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGCAG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775083.1
CDS	296..2356
Translation	MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_173060.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173060.4

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT 
TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG 
TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT 
CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG 
AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA 
GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA 
GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA 
CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT 
CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA 
GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT 
GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG 
GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG 
AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC 
AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG 
CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA 
CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGCAG 
AGTGACAAAG ACCTCGATGA TGCCTTGGAT AAACTCTCTG ACAGTCTAGG ACAAAGGCAG 
CCTGACCCAG ATGAGAACAA ACCAATGGAA GATAAAGTAA AGGAAAAAGC TAAAGCTGAA 
CATAGAGACA AGCTTGGAGA AAGAGATGAC ACTATCCCAC CTGAATACAG ACATCTCCTG 
GATGATAATG GACAGGACAA ACCAGTGAAG CCACCTACAA AGAAATCAGA GGATTCAAAG 
AAACCTGCAG ATGACCAAGA CCCCATTGAT GCTCTCTCAG GAGATCTGGA CAGCTGTCCC 
TCCACTACAG AAACCTCACA GAACACAGCA AAGGATAAGT GCAAGAAGGC TGCTTCCAGC 
TCCAAAGCAC CTAAGAATGG AGGTAAAGCG AAGGATTCAG CAAAGACAAC AGAGGAAACT 
TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042444.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035909.1 Homo sapiens calpastatin (CAST), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu39740
Clone ID Related Accession (Same CDS sequence)	NM_001042444.2
Accession Version	NM_001042444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714711.3, NM_001042444.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.8993.1, SRR1803612.218850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC 
TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT 
GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA 
GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG 
GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG 
CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA 
GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA 
AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG 
CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT 
AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT 
CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG 
CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG 
GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG 
GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT 
AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA 
CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT 
CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA 
GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG 
CAGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG 
CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT 
GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC 
CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA 
AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT 
CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC 
AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA 
ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035909.1
CDS	296..2299
Translation	MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042444.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042444.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC 
TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT 
GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA 
GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG 
GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG 
CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA 
GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA 
AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG 
CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT 
AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT 
CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG 
CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG 
GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG 
GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT 
AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA 
CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT 
CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA 
GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG 
CAGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG 
CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT 
GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC 
CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA 
AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT 
CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC 
AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA 
ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714715.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714778.1 Homo sapiens calpastatin (CAST), transcript variant X47, mRNA.

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CloneID	OHu39744
Clone ID Related Accession (Same CDS sequence)	XM_006714715.3
Accession Version	XM_006714715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X44
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT 
GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA 
GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG 
GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA 
TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA 
TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG 
GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG 
AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA 
GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA 
GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC 
ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT 
TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA 
GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA 
GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC 
AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT 
GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA 
GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA 
GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA 
CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA 
AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714778.1
CDS	838..2109
Translation	MSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714715.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714715.3

ORF Insert Sequence:

ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT 
GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA 
GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG 
GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA 
TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA 
TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG 
GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG 
AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA 
GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA 
GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC 
ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT 
TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA 
GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA 
GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC 
AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT 
GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA 
GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA 
GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA 
CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA 
AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001284213.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271142.1 Homo sapiens calpastatin (CAST), transcript variant 14, mRNA.

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CloneID	OHu21399
Clone ID Related Accession (Same CDS sequence)	NM_001284213.3
Accession Version	NM_001284213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	calpastatin isoform o
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AK300624.1, KC877037.1, BQ940454.1, CA775469.1 and BM676369.1. On Aug 30, 2016 this sequence version replaced NM_001284213.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300624.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGTCAGGC 
ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA 
GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG 
GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAAAAG 
GAAGGGATCA CAGGGCCTCC TGCAGACTCT TCGAAACCCA TAGGGCCAGA TGATGCTATA 
GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA 
AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT 
CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG 
GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT 
AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT 
GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA 
TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT 
TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA 
GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG 
TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA 
CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG 
CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG 
GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT 
GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA 
GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271142.1
CDS	296..2206
Translation	MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001284213.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284213.3

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGTCAGGC 
ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA 
GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG 
GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAAAAG 
GAAGGGATCA CAGGGCCTCC TGCAGACTCT TCGAAACCCA TAGGGCCAGA TGATGCTATA 
GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA 
AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT 
CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG 
GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT 
AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT 
GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA 
TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT 
TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA 
GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG 
TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA 
CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG 
CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG 
GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT 
GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA 
GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042446.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035911.1 Homo sapiens calpastatin (CAST), transcript variant 12, mRNA.

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CloneID	OHu39741
Clone ID Related Accession (Same CDS sequence)	NM_001042446.2
Accession Version	NM_001042446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	calpastatin isoform l
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714712.3, NM_001042446.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.66674.1, SRR1803614.37703.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA CCCATAGGGC CAGATGATGC TATAGACGCC 
TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG 
GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC 
CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG 
TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA 
GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA 
ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA 
GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA 
TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT 
ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG 
ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC 
CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT 
TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA 
CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA 
GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC 
TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA 
GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC 
CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT 
ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC 
TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT 
AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT 
TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035911.1
CDS	296..2260
Translation	MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042446.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042446.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA CCCATAGGGC CAGATGATGC TATAGACGCC 
TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG 
GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC 
CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG 
TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA 
GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA 
ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA 
GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA 
TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT 
ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG 
ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC 
CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT 
TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA 
CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA 
GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC 
TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA 
GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC 
CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT 
ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC 
TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT 
AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT 
TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714713.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714776.1 Homo sapiens calpastatin (CAST), transcript variant X45, mRNA.

Price & Availability↑

CloneID	OHu39742
Clone ID Related Accession (Same CDS sequence)	XM_006714713.3
Accession Version	XM_006714713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X42
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGT TTCTATCTTC GACTTTCTTG GAGGGCTCAC CGGCCACAGT GTGGCACGAT 
AAGCTTTGTG ACGGTGAACG CAGAGGAGCA AGAGAAGCAG TTCGTATCTT CCAGGACCAA 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714776.1
CDS	88..1383
Translation	MGQFLSSTFLEGSPATVWHDKLCDGERRGAREAVRIFQDQAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714713.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714713.3

ORF Insert Sequence:

ATGGGCCAGT TTCTATCTTC GACTTTCTTG GAGGGCTCAC CGGCCACAGT GTGGCACGAT 
AAGCTTTGTG ACGGTGAACG CAGAGGAGCA AGAGAAGCAG TTCGTATCTT CCAGGACCAA 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714714.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714777.1 Homo sapiens calpastatin (CAST), transcript variant X46, mRNA.

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CloneID	OHu39743
Clone ID Related Accession (Same CDS sequence)	XM_006714714.3
Accession Version	XM_006714714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X43
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAGT TTCTATCTTC GACTTTCTTG GAGGGCTCAC CGGCCACAGT GTGGCACGAT 
AAGCTTTGTG ACGGTGAACG CAGAGGAGCA AGAGAAGCAG TTCGTATCTT CCAGGACCAA 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714777.1
CDS	88..1380
Translation	MGQFLSSTFLEGSPATVWHDKLCDGERRGAREAVRIFQDQAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714714.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714714.3

ORF Insert Sequence:

ATGGGCCAGT TTCTATCTTC GACTTTCTTG GAGGGCTCAC CGGCCACAGT GTGGCACGAT 
AAGCTTTGTG ACGGTGAACG CAGAGGAGCA AGAGAAGCAG TTCGTATCTT CCAGGACCAA 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_011543658.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541960.1 Homo sapiens calpastatin (CAST), transcript variant X42, mRNA.

Price & Availability↑

CloneID	OHu56646
Clone ID Related Accession (Same CDS sequence)	XM_011543658.2
Accession Version	XM_011543658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGAAAAAA 
GAAAAGAAAT CATTAACCCC AGCTGTGCCA GTTGAATCTA AACCGGATAA ACCATCGGGA 
AAGTCAGGCA TGGATGCTGC TTTGGATGAC TTAATAGATA CTTTAGGAGG ACCTGAAGAA 
ACTGAAGAAG AAAATACAAC GTATACTGGA CCAGAAGTTT CAGATCCAAT GAGTTCCACC 
TACATAGAGG AATTGGGTAA AAGAGAAGTC ACAATTCCTC CAAAATATAG GGAACTATTG 
GCTAAAAAGG AAGGGATCAC AGGGCCTCCT GCAGACTCTT CGAAACCCAT AGGGCCAGAT 
GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG 
AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA 
AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA 
GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC 
CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT 
GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA 
AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT 
GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT 
GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG 
ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA 
GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC 
TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA 
TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA 
ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT 
GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA 
CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA 
AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA 
CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC 
CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG 
AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA 
GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541960.1
CDS	169..2148
Translation	MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_011543658.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543658.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGAAAAAA 
GAAAAGAAAT CATTAACCCC AGCTGTGCCA GTTGAATCTA AACCGGATAA ACCATCGGGA 
AAGTCAGGCA TGGATGCTGC TTTGGATGAC TTAATAGATA CTTTAGGAGG ACCTGAAGAA 
ACTGAAGAAG AAAATACAAC GTATACTGGA CCAGAAGTTT CAGATCCAAT GAGTTCCACC 
TACATAGAGG AATTGGGTAA AAGAGAAGTC ACAATTCCTC CAAAATATAG GGAACTATTG 
GCTAAAAAGG AAGGGATCAC AGGGCCTCCT GCAGACTCTT CGAAACCCAT AGGGCCAGAT 
GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG 
AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA 
AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA 
GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC 
CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT 
GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA 
AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT 
GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT 
GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG 
ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA 
GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC 
TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA 
TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA 
ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT 
GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA 
CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA 
AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA 
CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC 
CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG 
AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA 
GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330631.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317560.1 Homo sapiens calpastatin (CAST), transcript variant 22, mRNA.

Price & Availability↑

CloneID	OHu21220
Clone ID Related Accession (Same CDS sequence)	NM_001330631.1 , XM_006714708.2 , NM_001190442.1
Accession Version	NM_001330631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	calpastatin isoform m
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_006714708.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.23395.1, SRR1803611.253714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317560.1
CDS	296..2383
Translation	MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330631.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330631.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001190442.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177371.1 Homo sapiens calpastatin (CAST), transcript variant 16, mRNA.

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CloneID	OHu21220
Clone ID Related Accession (Same CDS sequence)	NM_001330631.1 , XM_006714708.2 , NM_001190442.1
Accession Version	NM_001190442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	calpastatin isoform m
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB450697.1, AC020900.5, AB208816.1, AC008906.5, AL832349.1 and AY189741.1. This sequence is a reference standard in the RefSeqGene project. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177371.1
CDS	304..2391
Translation	MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001190442.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190442.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330628.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317557.1 Homo sapiens calpastatin (CAST), transcript variant 19, mRNA.

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CloneID	OHu39736
Clone ID Related Accession (Same CDS sequence)	NM_001330628.1 , NM_001330628.2
Accession Version	NM_001330628.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	calpastatin isoform r
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_006714706.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.44671.1, SRR1803614.155067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA 
GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA 
GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT 
GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA 
TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG 
GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA 
AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA 
TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAAAAGGAA 
GGGATCACAG GGCCTCCTGC AGACTCTTCG AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317557.1
CDS	159..2366
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330628.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330628.1

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA 
GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA 
GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT 
GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA 
TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG 
GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA 
AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA 
TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAAAAGGAA 
GGGATCACAG GGCCTCCTGC AGACTCTTCG AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330628.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317557.1 Homo sapiens calpastatin (CAST), transcript variant 19, mRNA.

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CloneID	OHu39736
Clone ID Related Accession (Same CDS sequence)	NM_001330628.1 , NM_001330628.2
Accession Version	NM_001330628.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform r
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001330628.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.44671.1, SRR1803614.155067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA 
GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA 
GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT 
GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA 
TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG 
GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA 
AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA 
TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAAAAGGAA 
GGGATCACAG GGCCTCCTGC AGACTCTTCG AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317557.1
CDS	113..2320
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330628.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330628.2

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA 
GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA 
GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT 
GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA 
TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG 
GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA 
AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA 
TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAAAAGGAA 
GGGATCACAG GGCCTCCTGC AGACTCTTCG AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042443.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035908.1 Homo sapiens calpastatin (CAST), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu39737
Clone ID Related Accession (Same CDS sequence)	NM_001042443.2
Accession Version	NM_001042443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714707.3, NM_001042443.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.140040.1, SRR1803612.274671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAAAAGGAAG GGATCACAGG GCCTCCTGCA 
GACTCTTCGA AACCCATAGG GCCAGATGAT GCTATAGACG CCTTGTCATC TGACTTCACC 
TGTGGGTCGC CTACAGCTGC TGGAAAGAAA ACTGAAAAAG AGGAATCTAC AGAAGTTTTA 
AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG 
GTGGAGAAGG ATACAATGAG TGATCAAGCA CTCGAGGCTC TGTCGGCTTC ACTGGGCACC 
CGGCAAGCAG AACCTGAGCT CGACCTCCGC TCAATTAAGG AAGTCGATGA GGCAAAAGCT 
AAAGAAGAAA AACTAGAGAA GTGTGGTGAG GATGATGAAA CAATCCCATC TGAGTACAGA 
TTAAAACCAG CCACGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAAAACCC 
AAGCCTCGGA GTGAATCAGA ACTCATTGAT GAACTTTCAG AAGATTTTGA CCGGTCTGAA 
TGTAAAGAGA AACCATCTAA GCCAACTGAA AAGACAGAAG AATCTAAGGC CGCTGCTCCA 
GCTCCTGTGT CGGAGGCTGT GTGTCGGACC TCCATGTGTA GTATACAGTC AGCACCCCCT 
GAGCCGGCTA CCTTGAAGGG CACAGTGCCA GATGATGCTG TAGAAGCCTT GGCTGATAGC 
CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT GGAAAACCTG TGATGGATAA AGTCAAGGAG 
AAGGCCAAAG AAGAAGACCG TGAAAAGCTT GGTGAAAAAG AAGAAACAAT TCCTCCTGAT 
TATAGATTAG AAGAGGTCAA GGATAAAGAT GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG 
GAACAGCTTC CACCCATGAG TGAAGACTTC CTTCTGGATG CTTTGTCTGA GGACTTCTCT 
GGTCCACAAA ATGCTTCATC TCTTAAATTT GAAGATGCTA AACTTGCTGC TGCCATCTCT 
GAAGTGGTTT CCCAAACCCC AGCTTCAACG ACCCAAGCTG GAGCCCCACC CCGTGATACC 
TCGCAGAGTG ACAAAGACCT CGATGATGCC TTGGATAAAC TCTCTGACAG TCTAGGACAA 
AGGCAGCCTG ACCCAGATGA GAACAAACCA ATGGAAGATA AAGTAAAGGA AAAAGCTAAA 
GCTGAACATA GAGACAAGCT TGGAGAAAGA GATGACACTA TCCCACCTGA ATACAGACAT 
CTCCTGGATG ATAATGGACA GGACAAACCA GTGAAGCCAC CTACAAAGAA ATCAGAGGAT 
TCAAAGAAAC CTGCAGATGA CCAAGACCCC ATTGATGCTC TCTCAGGAGA TCTGGACAGC 
TGTCCCTCCA CTACAGAAAC CTCACAGAAC ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT 
TCCAGCTCCA AAGCACCTAA GAATGGAGGT AAAGCGAAGG ATTCAGCAAA GACAACAGAG 
GAAACTTCCA AGCCAAAAGA TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035908.1
CDS	296..2422
Translation	MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042443.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042443.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAAAAGGAAG GGATCACAGG GCCTCCTGCA 
GACTCTTCGA AACCCATAGG GCCAGATGAT GCTATAGACG CCTTGTCATC TGACTTCACC 
TGTGGGTCGC CTACAGCTGC TGGAAAGAAA ACTGAAAAAG AGGAATCTAC AGAAGTTTTA 
AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG 
GTGGAGAAGG ATACAATGAG TGATCAAGCA CTCGAGGCTC TGTCGGCTTC ACTGGGCACC 
CGGCAAGCAG AACCTGAGCT CGACCTCCGC TCAATTAAGG AAGTCGATGA GGCAAAAGCT 
AAAGAAGAAA AACTAGAGAA GTGTGGTGAG GATGATGAAA CAATCCCATC TGAGTACAGA 
TTAAAACCAG CCACGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAAAACCC 
AAGCCTCGGA GTGAATCAGA ACTCATTGAT GAACTTTCAG AAGATTTTGA CCGGTCTGAA 
TGTAAAGAGA AACCATCTAA GCCAACTGAA AAGACAGAAG AATCTAAGGC CGCTGCTCCA 
GCTCCTGTGT CGGAGGCTGT GTGTCGGACC TCCATGTGTA GTATACAGTC AGCACCCCCT 
GAGCCGGCTA CCTTGAAGGG CACAGTGCCA GATGATGCTG TAGAAGCCTT GGCTGATAGC 
CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT GGAAAACCTG TGATGGATAA AGTCAAGGAG 
AAGGCCAAAG AAGAAGACCG TGAAAAGCTT GGTGAAAAAG AAGAAACAAT TCCTCCTGAT 
TATAGATTAG AAGAGGTCAA GGATAAAGAT GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG 
GAACAGCTTC CACCCATGAG TGAAGACTTC CTTCTGGATG CTTTGTCTGA GGACTTCTCT 
GGTCCACAAA ATGCTTCATC TCTTAAATTT GAAGATGCTA AACTTGCTGC TGCCATCTCT 
GAAGTGGTTT CCCAAACCCC AGCTTCAACG ACCCAAGCTG GAGCCCCACC CCGTGATACC 
TCGCAGAGTG ACAAAGACCT CGATGATGCC TTGGATAAAC TCTCTGACAG TCTAGGACAA 
AGGCAGCCTG ACCCAGATGA GAACAAACCA ATGGAAGATA AAGTAAAGGA AAAAGCTAAA 
GCTGAACATA GAGACAAGCT TGGAGAAAGA GATGACACTA TCCCACCTGA ATACAGACAT 
CTCCTGGATG ATAATGGACA GGACAAACCA GTGAAGCCAC CTACAAAGAA ATCAGAGGAT 
TCAAAGAAAC CTGCAGATGA CCAAGACCCC ATTGATGCTC TCTCAGGAGA TCTGGACAGC 
TGTCCCTCCA CTACAGAAAC CTCACAGAAC ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT 
TCCAGCTCCA AAGCACCTAA GAATGGAGGT AAAGCGAAGG ATTCAGCAAA GACAACAGAG 
GAAACTTCCA AGCCAAAAGA TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330633.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317562.1 Homo sapiens calpastatin (CAST), transcript variant 24, mRNA.

Price & Availability↑

CloneID	OHu39738
Clone ID Related Accession (Same CDS sequence)	NM_001330633.1
Accession Version	NM_001330633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	calpastatin isoform v
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_006714709.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.166555.1, SRR1660809.94270.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317562.1
CDS	296..2365
Translation	MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330633.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330633.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042445.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035910.1 Homo sapiens calpastatin (CAST), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu39739
Clone ID Related Accession (Same CDS sequence)	NM_001042445.2
Accession Version	NM_001042445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform k
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714710.3, NM_001042445.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.102275.1, SRR1660803.123392.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAACCC ATAGGGCCAG ATGATGCTAT AGACGCCTTG 
TCATCTGACT TCACCTGTGG GTCGCCTACA GCTGCTGGAA AGAAAACTGA AAAAGAGGAA 
TCTACAGAAG TTTTAAAAGC TCAGTCAGCA GGGACAGTCA GAAGTGCTGC TCCACCCCAA 
GAGAAGAAAA GAAAGGTGGA GAAGGATACA ATGAGTGATC AAGCACTCGA GGCTCTGTCG 
GCTTCACTGG GCACCCGGCA AGCAGAACCT GAGCTCGACC TCCGCTCAAT TAAGGAAGTC 
GATGAGGCAA AAGCTAAAGA AGAAAAACTA GAGAAGTGTG GTGAGGATGA TGAAACAATC 
CCATCTGAGT ACAGATTAAA ACCAGCCACG GATAAAGATG GAAAACCACT ATTGCCAGAG 
CCTGAAGAAA AACCCAAGCC TCGGAGTGAA TCAGAACTCA TTGATGAACT TTCAGAAGAT 
TTTGACCGGT CTGAATGTAA AGAGAAACCA TCTAAGCCAA CTGAAAAGAC AGAAGAATCT 
AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG GCTGTGTGTC GGACCTCCAT GTGTAGTATA 
CAGTCAGCAC CCCCTGAGCC GGCTACCTTG AAGGGCACAG TGCCAGATGA TGCTGTAGAA 
GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA GCAGATCCAG AAGATGGAAA ACCTGTGATG 
GATAAAGTCA AGGAGAAGGC CAAAGAAGAA GACCGTGAAA AGCTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA GCCACTCCTG 
CCAAAAGAGT CTAAGGAACA GCTTCCACCC ATGAGTGAAG ACTTCCTTCT GGATGCTTTG 
TCTGAGGACT TCTCTGGTCC ACAAAATGCT TCATCTCTTA AATTTGAAGA TGCTAAACTT 
GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTT CAACGACCCA AGCTGGAGCC 
CCACCCCGTG ATACCTCGCA GAGTGACAAA GACCTCGATG ATGCCTTGGA TAAACTCTCT 
GACAGTCTAG GACAAAGGCA GCCTGACCCA GATGAGAACA AACCAATGGA AGATAAAGTA 
AAGGAAAAAG CTAAAGCTGA ACATAGAGAC AAGCTTGGAG AAAGAGATGA CACTATCCCA 
CCTGAATACA GACATCTCCT GGATGATAAT GGACAGGACA AACCAGTGAA GCCACCTACA 
AAGAAATCAG AGGATTCAAA GAAACCTGCA GATGACCAAG ACCCCATTGA TGCTCTCTCA 
GGAGATCTGG ACAGCTGTCC CTCCACTACA GAAACCTCAC AGAACACAGC AAAGGATAAG 
TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA CCTAAGAATG GAGGTAAAGC GAAGGATTCA 
GCAAAGACAA CAGAGGAAAC TTCCAAGCCA AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035910.1
CDS	296..2317
Translation	MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042445.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042445.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAACCC ATAGGGCCAG ATGATGCTAT AGACGCCTTG 
TCATCTGACT TCACCTGTGG GTCGCCTACA GCTGCTGGAA AGAAAACTGA AAAAGAGGAA 
TCTACAGAAG TTTTAAAAGC TCAGTCAGCA GGGACAGTCA GAAGTGCTGC TCCACCCCAA 
GAGAAGAAAA GAAAGGTGGA GAAGGATACA ATGAGTGATC AAGCACTCGA GGCTCTGTCG 
GCTTCACTGG GCACCCGGCA AGCAGAACCT GAGCTCGACC TCCGCTCAAT TAAGGAAGTC 
GATGAGGCAA AAGCTAAAGA AGAAAAACTA GAGAAGTGTG GTGAGGATGA TGAAACAATC 
CCATCTGAGT ACAGATTAAA ACCAGCCACG GATAAAGATG GAAAACCACT ATTGCCAGAG 
CCTGAAGAAA AACCCAAGCC TCGGAGTGAA TCAGAACTCA TTGATGAACT TTCAGAAGAT 
TTTGACCGGT CTGAATGTAA AGAGAAACCA TCTAAGCCAA CTGAAAAGAC AGAAGAATCT 
AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG GCTGTGTGTC GGACCTCCAT GTGTAGTATA 
CAGTCAGCAC CCCCTGAGCC GGCTACCTTG AAGGGCACAG TGCCAGATGA TGCTGTAGAA 
GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA GCAGATCCAG AAGATGGAAA ACCTGTGATG 
GATAAAGTCA AGGAGAAGGC CAAAGAAGAA GACCGTGAAA AGCTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA GCCACTCCTG 
CCAAAAGAGT CTAAGGAACA GCTTCCACCC ATGAGTGAAG ACTTCCTTCT GGATGCTTTG 
TCTGAGGACT TCTCTGGTCC ACAAAATGCT TCATCTCTTA AATTTGAAGA TGCTAAACTT 
GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTT CAACGACCCA AGCTGGAGCC 
CCACCCCGTG ATACCTCGCA GAGTGACAAA GACCTCGATG ATGCCTTGGA TAAACTCTCT 
GACAGTCTAG GACAAAGGCA GCCTGACCCA GATGAGAACA AACCAATGGA AGATAAAGTA 
AAGGAAAAAG CTAAAGCTGA ACATAGAGAC AAGCTTGGAG AAAGAGATGA CACTATCCCA 
CCTGAATACA GACATCTCCT GGATGATAAT GGACAGGACA AACCAGTGAA GCCACCTACA 
AAGAAATCAG AGGATTCAAA GAAACCTGCA GATGACCAAG ACCCCATTGA TGCTCTCTCA 
GGAGATCTGG ACAGCTGTCC CTCCACTACA GAAACCTCAC AGAACACAGC AAAGGATAAG 
TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA CCTAAGAATG GAGGTAAAGC GAAGGATTCA 
GCAAAGACAA CAGAGGAAAC TTCCAAGCCA AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714708.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714771.1 Homo sapiens calpastatin (CAST), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu21220
Clone ID Related Accession (Same CDS sequence)	NM_001330631.1 , XM_006714708.2 , NM_001190442.1
Accession Version	XM_006714708.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	calpastatin isoform X1
Comment

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006714771.1

Translation

MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714708.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714708.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGCAGAGT GACAAAGACC TCGATGATGC CTTGGATAAA 
CTCTCTGACA GTCTAGGACA AAGGCAGCCT GACCCAGATG AGAACAAACC AATGGAAGAT 
AAAGTAAAGG AAAAAGCTAA AGCTGAACAT AGAGACAAGC TTGGAGAAAG AGATGACACT 
ATCCCACCTG AATACAGACA TCTCCTGGAT GATAATGGAC AGGACAAACC AGTGAAGCCA 
CCTACAAAGA AATCAGAGGA TTCAAAGAAA CCTGCAGATG ACCAAGACCC CATTGATGCT 
CTCTCAGGAG ATCTGGACAG CTGTCCCTCC ACTACAGAAA CCTCACAGAA CACAGCAAAG 
GATAAGTGCA AGAAGGCTGC TTCCAGCTCC AAAGCACCTA AGAATGGAGG TAAAGCGAAG 
GATTCAGCAA AGACAACAGA GGAAACTTCC AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714699.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714762.1 Homo sapiens calpastatin (CAST), transcript variant X6, mRNA.

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CloneID	OHu39729
Clone ID Related Accession (Same CDS sequence)	XM_006714699.3
Accession Version	XM_006714699.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC 
CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA 
ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC 
CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT 
ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA 
TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG 
AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA 
TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC 
ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA 
GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG 
GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAAAAG 
GAAGGGATCA CAGGGCCTCC TGCAGACTCT TCGAAACCCA TAGGGCCAGA TGATGCTATA 
GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA 
AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT 
CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG 
GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT 
AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT 
GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA 
TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT 
TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA 
GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG 
TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA 
CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG 
CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG 
GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT 
GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA 
GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714762.1
CDS	144..2474
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714699.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714699.3

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC 
CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA 
ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC 
CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT 
ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA 
TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG 
AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA 
TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC 
ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA 
GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG 
GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAAAAG 
GAAGGGATCA CAGGGCCTCC TGCAGACTCT TCGAAACCCA TAGGGCCAGA TGATGCTATA 
GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA 
AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT 
CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG 
GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT 
AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT 
GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA 
TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT 
TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA 
GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG 
TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA 
CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG 
CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG 
GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT 
GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA 
GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714702.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714765.1 Homo sapiens calpastatin (CAST), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu39732
Clone ID Related Accession (Same CDS sequence)	XM_006714702.3
Accession Version	XM_006714702.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714702.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC 
CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA 
ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCCAAAAA GCCTACCCAA GCAGGCATCA 
GATACAGGAA GTAACGATGC TCACAATAAA AAAGCAGTTT CCAGATCAGC TGAACAGCAG 
CCATCAGAGA AATCAACAGA ACCAAAGACT AAACCACAAG ACATGATTTC TGCTGGTGGA 
GAGAGTGTTG CTGGTATCAC TGCAATATCT GGCAAGCCGG GTGACAAGAA AAAAGAAAAG 
AAATCATTAA CCCCAGCTGT GCCAGTTGAA TCTAAACCGG ATAAACCATC GGGAAAGTCA 
GGCATGGATG CTGCTTTGGA TGACTTAATA GATACTTTAG GAGGACCTGA AGAAACTGAA 
GAAGAAAATA CAACGTATAC TGGACCAGAA GTTTCAGATC CAATGAGTTC CACCTACATA 
GAGGAATTGG GTAAAAGAGA AGTCACAATT CCTCCAAAAT ATAGGGAACT ATTGGCTAAA 
AAGGAAGGGA TCACAGGGCC TCCTGCAGAC TCTTCGAAAC CCATAGGGCC AGATGATGCT 
ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT 
GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT 
GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC 
GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA 
ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT 
GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA 
CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA 
CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG 
ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC 
ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA 
AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT 
CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA 
GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC 
CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA TGATGCCTTG 
GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG 
GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT 
GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG 
AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT 
GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA 
GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA 
GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714765.1
CDS	112..2385
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714702.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714702.3

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC 
CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA 
ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCCAAAAA GCCTACCCAA GCAGGCATCA 
GATACAGGAA GTAACGATGC TCACAATAAA AAAGCAGTTT CCAGATCAGC TGAACAGCAG 
CCATCAGAGA AATCAACAGA ACCAAAGACT AAACCACAAG ACATGATTTC TGCTGGTGGA 
GAGAGTGTTG CTGGTATCAC TGCAATATCT GGCAAGCCGG GTGACAAGAA AAAAGAAAAG 
AAATCATTAA CCCCAGCTGT GCCAGTTGAA TCTAAACCGG ATAAACCATC GGGAAAGTCA 
GGCATGGATG CTGCTTTGGA TGACTTAATA GATACTTTAG GAGGACCTGA AGAAACTGAA 
GAAGAAAATA CAACGTATAC TGGACCAGAA GTTTCAGATC CAATGAGTTC CACCTACATA 
GAGGAATTGG GTAAAAGAGA AGTCACAATT CCTCCAAAAT ATAGGGAACT ATTGGCTAAA 
AAGGAAGGGA TCACAGGGCC TCCTGCAGAC TCTTCGAAAC CCATAGGGCC AGATGATGCT 
ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT 
GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT 
GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC 
GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA 
ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT 
GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA 
CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA 
CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG 
ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC 
ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA 
AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT 
CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA 
GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC 
CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA TGATGCCTTG 
GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG 
GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT 
GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG 
AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT 
GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA 
GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA 
GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714704.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714767.1 Homo sapiens calpastatin (CAST), transcript variant X19, mRNA.

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CloneID	OHu39734
Clone ID Related Accession (Same CDS sequence)	XM_006714704.3 , NM_001375317.1
Accession Version	XM_006714704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT 
GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA 
ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT 
GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT 
ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG 
GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA AAAGGAAGGG 
ATCACAGGGC CTCCTGCAGA CTCTTCGAAA CCCATAGGGC CAGATGATGC TATAGACGCC 
TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG 
GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC 
CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG 
TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA 
GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA 
ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA 
GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA 
TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT 
ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG 
ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC 
CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT 
TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA 
CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA 
GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC 
TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA 
GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC 
CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT 
ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC 
TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT 
AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT 
TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714767.1
CDS	154..2418
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714704.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714704.3

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT 
GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA 
ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT 
GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT 
ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG 
GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA AAAGGAAGGG 
ATCACAGGGC CTCCTGCAGA CTCTTCGAAA CCCATAGGGC CAGATGATGC TATAGACGCC 
TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG 
GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC 
CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG 
TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA 
GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA 
ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA 
GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA 
TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT 
ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG 
ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC 
CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT 
TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA 
CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA 
GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC 
TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA 
GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC 
CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT 
ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC 
TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT 
AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT 
TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_011543657.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541959.1 Homo sapiens calpastatin (CAST), transcript variant X29, mRNA.

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CloneID	OHu56645
Clone ID Related Accession (Same CDS sequence)	XM_011543657.2
Accession Version	XM_011543657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC 
TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT 
GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA 
GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG 
GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG 
CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA 
GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA 
AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG 
CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT 
AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT 
CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG 
CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG 
GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG 
GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT 
AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA 
CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT 
CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA 
GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG 
CAGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG 
CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT 
GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC 
CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA 
AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT 
CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC 
AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA 
ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541959.1
CDS	112..2295
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_011543657.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543657.2

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA AAGGAAGGGA TCACAGGGCC TCCTGCAGAC 
TCTTCGAAAC CCATAGGGCC AGATGATGCT ATAGACGCCT TGTCATCTGA CTTCACCTGT 
GGGTCGCCTA CAGCTGCTGG AAAGAAAACT GAAAAAGAGG AATCTACAGA AGTTTTAAAA 
GCTCAGTCAG CAGGGACAGT CAGAAGTGCT GCTCCACCCC AAGAGAAGAA AAGAAAGGTG 
GAGAAGGATA CAATGAGTGA TCAAGCACTC GAGGCTCTGT CGGCTTCACT GGGCACCCGG 
CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA ATTAAGGAAG TCGATGAGGC AAAAGCTAAA 
GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT GATGAAACAA TCCCATCTGA GTACAGATTA 
AAACCAGCCA CGGATAAAGA TGGAAAACCA CTATTGCCAG AGCCTGAAGA AAAACCCAAG 
CCTCGGAGTG AATCAGAACT CATTGATGAA CTTTCAGAAG ATTTTGACCG GTCTGAATGT 
AAAGAGAAAC CATCTAAGCC AACTGAAAAG ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT 
CCTGTGTCGG AGGCTGTGTG TCGGACCTCC ATGTGTAGTA TACAGTCAGC ACCCCCTGAG 
CCGGCTACCT TGAAGGGCAC AGTGCCAGAT GATGCTGTAG AAGCCTTGGC TGATAGCCTG 
GGGAAAAAGG AAGCAGATCC AGAAGATGGA AAACCTGTGA TGGATAAAGT CAAGGAGAAG 
GCCAAAGAAG AAGACCGTGA AAAGCTTGGT GAAAAAGAAG AAACAATTCC TCCTGATTAT 
AGATTAGAAG AGGTCAAGGA TAAAGATGGA AAGCCACTCC TGCCAAAAGA GTCTAAGGAA 
CAGCTTCCAC CCATGAGTGA AGACTTCCTT CTGGATGCTT TGTCTGAGGA CTTCTCTGGT 
CCACAAAATG CTTCATCTCT TAAATTTGAA GATGCTAAAC TTGCTGCTGC CATCTCTGAA 
GTGGTTTCCC AAACCCCAGC TTCAACGACC CAAGCTGGAG CCCCACCCCG TGATACCTCG 
CAGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG 
CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT 
GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC 
CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA 
AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT 
CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC 
AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA 
ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330630.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317559.1 Homo sapiens calpastatin (CAST), transcript variant 21, mRNA.

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CloneID	OHu102190
Clone ID Related Accession (Same CDS sequence)	NM_001330630.1
Accession Version	NM_001330630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform t
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009932.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.205463.1, SRR1803616.10936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA CCCATAGGGC CAGATGATGC TATAGACGCC 
TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG 
GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC 
CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG 
TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA 
GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA 
ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA 
GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA 
TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT 
ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG 
ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC 
CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT 
TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA 
CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA 
GCCCCACCCC GTGATACCTC GAGTGACAAA GACCTCGATG ATGCCTTGGA TAAACTCTCT 
GACAGTCTAG GACAAAGGCA GCCTGACCCA GATGAGAACA AACCAATGGA AGATAAAGTA 
AAGGAAAAAG CTAAAGCTGA ACATAGAGAC AAGCTTGGAG AAAGAGATGA CACTATCCCA 
CCTGAATACA GACATCTCCT GGATGATAAT GGACAGGACA AACCAGTGAA GCCACCTACA 
AAGAAATCAG AGGATTCAAA GAAACCTGCA GATGACCAAG ACCCCATTGA TGCTCTCTCA 
GGAGATCTGG ACAGCTGTCC CTCCACTACA GAAACCTCAC AGAACACAGC AAAGGATAAG 
TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA CCTAAGAATG GAGGTAAAGC GAAGGATTCA 
GCAAAGACAA CAGAGGAAAC TTCCAAGCCA AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317559.1
CDS	296..2257
Translation	MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330630.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330630.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCCAAAAA GCCTACCCAA GCAGGCATCA GATACAGGAA GTAACGATGC TCACAATAAA 
AAAGCAGTTT CCAGATCAGC TGAACAGCAG CCATCAGAGA AATCAACAGA ACCAAAGACT 
AAACCACAAG ACATGATTTC TGCTGGTGGA GAGAGTGTTG CTGGTATCAC TGCAATATCT 
GGCAAGCCGG GTGACAAGAA AAAAGAAAAG AAATCATTAA CCCCAGCTGT GCCAGTTGAA 
TCTAAACCGG ATAAACCATC GGGAAAGTCA GGCATGGATG CTGCTTTGGA TGACTTAATA 
GATACTTTAG GAGGACCTGA AGAAACTGAA GAAGAAAATA CAACGTATAC TGGACCAGAA 
GTTTCAGATC CAATGAGTTC CACCTACATA GAGGAATTGG GTAAAAGAGA AGTCACAATT 
CCTCCAAAAT ATAGGGAACT ATTGGCTAAA CCCATAGGGC CAGATGATGC TATAGACGCC 
TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG 
GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC 
CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG 
TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA 
GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA 
ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA 
GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA 
TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT 
ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG 
ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC 
CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT 
TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA 
CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA 
GCCCCACCCC GTGATACCTC GAGTGACAAA GACCTCGATG ATGCCTTGGA TAAACTCTCT 
GACAGTCTAG GACAAAGGCA GCCTGACCCA GATGAGAACA AACCAATGGA AGATAAAGTA 
AAGGAAAAAG CTAAAGCTGA ACATAGAGAC AAGCTTGGAG AAAGAGATGA CACTATCCCA 
CCTGAATACA GACATCTCCT GGATGATAAT GGACAGGACA AACCAGTGAA GCCACCTACA 
AAGAAATCAG AGGATTCAAA GAAACCTGCA GATGACCAAG ACCCCATTGA TGCTCTCTCA 
GGAGATCTGG ACAGCTGTCC CTCCACTACA GAAACCTCAC AGAACACAGC AAAGGATAAG 
TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA CCTAAGAATG GAGGTAAAGC GAAGGATTCA 
GCAAAGACAA CAGAGGAAAC TTCCAAGCCA AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330632.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317561.1 Homo sapiens calpastatin (CAST), transcript variant 23, mRNA.

Price & Availability↑

CloneID	OHu102283
Clone ID Related Accession (Same CDS sequence)	NM_001330632.1
Accession Version	NM_001330632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform u
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009929.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.176286.1, SRR1660803.69084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT 
TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG 
TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT 
CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG 
AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA 
GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA 
GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA 
CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT 
CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA 
GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT 
GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG 
GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG 
AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC 
AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG 
CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA 
CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGAGT 
GACAAAGACC TCGATGATGC CTTGGATAAA CTCTCTGACA GTCTAGGACA AAGGCAGCCT 
GACCCAGATG AGAACAAACC AATGGAAGAT AAAGTAAAGG AAAAAGCTAA AGCTGAACAT 
AGAGACAAGC TTGGAGAAAG AGATGACACT ATCCCACCTG AATACAGACA TCTCCTGGAT 
GATAATGGAC AGGACAAACC AGTGAAGCCA CCTACAAAGA AATCAGAGGA TTCAAAGAAA 
CCTGCAGATG ACCAAGACCC CATTGATGCT CTCTCAGGAG ATCTGGACAG CTGTCCCTCC 
ACTACAGAAA CCTCACAGAA CACAGCAAAG GATAAGTGCA AGAAGGCTGC TTCCAGCTCC 
AAAGCACCTA AGAATGGAGG TAAAGCGAAG GATTCAGCAA AGACAACAGA GGAAACTTCC 
AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317561.1
CDS	296..2353
Translation	MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330632.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330632.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAAAAG GAAGGGATCA CAGGGCCTCC TGCAGACTCT 
TCGAAACCCA TAGGGCCAGA TGATGCTATA GACGCCTTGT CATCTGACTT CACCTGTGGG 
TCGCCTACAG CTGCTGGAAA GAAAACTGAA AAAGAGGAAT CTACAGAAGT TTTAAAAGCT 
CAGTCAGCAG GGACAGTCAG AAGTGCTGCT CCACCCCAAG AGAAGAAAAG AAAGGTGGAG 
AAGGATACAA TGAGTGATCA AGCACTCGAG GCTCTGTCGG CTTCACTGGG CACCCGGCAA 
GCAGAACCTG AGCTCGACCT CCGCTCAATT AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA 
GAAAAACTAG AGAAGTGTGG TGAGGATGAT GAAACAATCC CATCTGAGTA CAGATTAAAA 
CCAGCCACGG ATAAAGATGG AAAACCACTA TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT 
CGGAGTGAAT CAGAACTCAT TGATGAACTT TCAGAAGATT TTGACCGGTC TGAATGTAAA 
GAGAAACCAT CTAAGCCAAC TGAAAAGACA GAAGAATCTA AGGCCGCTGC TCCAGCTCCT 
GTGTCGGAGG CTGTGTGTCG GACCTCCATG TGTAGTATAC AGTCAGCACC CCCTGAGCCG 
GCTACCTTGA AGGGCACAGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG 
AAAAAGGAAG CAGATCCAGA AGATGGAAAA CCTGTGATGG ATAAAGTCAA GGAGAAGGCC 
AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAG CCACTCCTGC CAAAAGAGTC TAAGGAACAG 
CTTCCACCCA TGAGTGAAGA CTTCCTTCTG GATGCTTTGT CTGAGGACTT CTCTGGTCCA 
CAAAATGCTT CATCTCTTAA ATTTGAAGAT GCTAAACTTG CTGCTGCCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTTC AACGACCCAA GCTGGAGCCC CACCCCGTGA TACCTCGAGT 
GACAAAGACC TCGATGATGC CTTGGATAAA CTCTCTGACA GTCTAGGACA AAGGCAGCCT 
GACCCAGATG AGAACAAACC AATGGAAGAT AAAGTAAAGG AAAAAGCTAA AGCTGAACAT 
AGAGACAAGC TTGGAGAAAG AGATGACACT ATCCCACCTG AATACAGACA TCTCCTGGAT 
GATAATGGAC AGGACAAACC AGTGAAGCCA CCTACAAAGA AATCAGAGGA TTCAAAGAAA 
CCTGCAGATG ACCAAGACCC CATTGATGCT CTCTCAGGAG ATCTGGACAG CTGTCCCTCC 
ACTACAGAAA CCTCACAGAA CACAGCAAAG GATAAGTGCA AGAAGGCTGC TTCCAGCTCC 
AAAGCACCTA AGAATGGAGG TAAAGCGAAG GATTCAGCAA AGACAACAGA GGAAACTTCC 
AAGCCAAAAG ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330634.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317563.1 Homo sapiens calpastatin (CAST), transcript variant 25, mRNA.

Price & Availability↑

CloneID	OHu102294
Clone ID Related Accession (Same CDS sequence)	NM_001330634.1
Accession Version	NM_001330634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform w
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009930.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.44180.1, SRR1803614.182278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAACCCA TAGGGCCAGA TGATGCTATA 
GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA 
AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT 
CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG 
GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT 
AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT 
GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA 
TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT 
TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA 
GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG 
TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA 
CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG 
CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG 
GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT 
GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA 
GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317563.1
CDS	296..2326
Translation	MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330634.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330634.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAACCCA TAGGGCCAGA TGATGCTATA 
GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA 
AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT 
CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG 
GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT 
AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT 
GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA 
TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT 
TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA 
GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG 
TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA 
CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG 
CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG 
GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT 
GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA 
GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330629.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317558.1 Homo sapiens calpastatin (CAST), transcript variant 20, mRNA.

Price & Availability↑

CloneID	OHu102380
Clone ID Related Accession (Same CDS sequence)	NM_001330629.1 , NM_001330629.2
Accession Version	NM_001330629.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	calpastatin isoform s
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009917.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.191419.1, SRR1803615.231733.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC 
CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA 
ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC 
CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT 
ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA 
TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG 
AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA 
TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC 
ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA 
GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG 
GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAACCC 
ATAGGGCCAG ATGATGCTAT AGACGCCTTG TCATCTGACT TCACCTGTGG GTCGCCTACA 
GCTGCTGGAA AGAAAACTGA AAAAGAGGAA TCTACAGAAG TTTTAAAAGC TCAGTCAGCA 
GGGACAGTCA GAAGTGCTGC TCCACCCCAA GAGAAGAAAA GAAAGGTGGA GAAGGATACA 
ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT 
GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA 
GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG 
GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA 
TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA 
TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG 
GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG 
AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA 
GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA 
GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC 
ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT 
TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA 
GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA 
GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC 
AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT 
GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA 
GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA 
GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA 
CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA 
AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317558.1
CDS	159..2450
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330629.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330629.1

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC 
CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA 
ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC 
CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT 
ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA 
TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG 
AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA 
TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC 
ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA 
GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG 
GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAACCC 
ATAGGGCCAG ATGATGCTAT AGACGCCTTG TCATCTGACT TCACCTGTGG GTCGCCTACA 
GCTGCTGGAA AGAAAACTGA AAAAGAGGAA TCTACAGAAG TTTTAAAAGC TCAGTCAGCA 
GGGACAGTCA GAAGTGCTGC TCCACCCCAA GAGAAGAAAA GAAAGGTGGA GAAGGATACA 
ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT 
GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA 
GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG 
GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA 
TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA 
TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG 
GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG 
AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA 
GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA 
GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC 
ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT 
TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA 
GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA 
GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC 
AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT 
GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA 
GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA 
GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA 
CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA 
AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330629.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317558.1 Homo sapiens calpastatin (CAST), transcript variant 20, mRNA.

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CloneID	OHu102380
Clone ID Related Accession (Same CDS sequence)	NM_001330629.1 , NM_001330629.2
Accession Version	NM_001330629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	calpastatin isoform s
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001330629.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.231733.1, SRR1803611.191419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC 
CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA 
ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC 
CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT 
ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA 
TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG 
AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA 
TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC 
ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA 
GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG 
GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAACCC 
ATAGGGCCAG ATGATGCTAT AGACGCCTTG TCATCTGACT TCACCTGTGG GTCGCCTACA 
GCTGCTGGAA AGAAAACTGA AAAAGAGGAA TCTACAGAAG TTTTAAAAGC TCAGTCAGCA 
GGGACAGTCA GAAGTGCTGC TCCACCCCAA GAGAAGAAAA GAAAGGTGGA GAAGGATACA 
ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT 
GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA 
GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG 
GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA 
TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA 
TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG 
GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG 
AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA 
GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA 
GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC 
ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT 
TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA 
GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA 
GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC 
AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT 
GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA 
GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA 
GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA 
CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA 
AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317558.1
CDS	113..2404
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330629.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330629.2

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCCAT TCCAGTCAGC 
CAACAGATGG AAGGACCACA TCTTCCTAAC AAGAAAAAAC ACAAAAAACA GGCTGTAAAA 
ACAGAACCTG AGAAGAAGTC ACAGTCAACC AAGCTGTCTG TGGTTCATGA GAAAAAATCC 
CAAGAAGGAA AGCCAAAAGA ACACACAGAG CCAAAAAGCC TACCCAAGCA GGCATCAGAT 
ACAGGAAGTA ACGATGCTCA CAATAAAAAA GCAGTTTCCA GATCAGCTGA ACAGCAGCCA 
TCAGAGAAAT CAACAGAACC AAAGACTAAA CCACAAGACA TGATTTCTGC TGGTGGAGAG 
AGTGTTGCTG GTATCACTGC AATATCTGGC AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA 
TCATTAACCC CAGCTGTGCC AGTTGAATCT AAACCGGATA AACCATCGGG AAAGTCAGGC 
ATGGATGCTG CTTTGGATGA CTTAATAGAT ACTTTAGGAG GACCTGAAGA AACTGAAGAA 
GAAAATACAA CGTATACTGG ACCAGAAGTT TCAGATCCAA TGAGTTCCAC CTACATAGAG 
GAATTGGGTA AAAGAGAAGT CACAATTCCT CCAAAATATA GGGAACTATT GGCTAAACCC 
ATAGGGCCAG ATGATGCTAT AGACGCCTTG TCATCTGACT TCACCTGTGG GTCGCCTACA 
GCTGCTGGAA AGAAAACTGA AAAAGAGGAA TCTACAGAAG TTTTAAAAGC TCAGTCAGCA 
GGGACAGTCA GAAGTGCTGC TCCACCCCAA GAGAAGAAAA GAAAGGTGGA GAAGGATACA 
ATGAGTGATC AAGCACTCGA GGCTCTGTCG GCTTCACTGG GCACCCGGCA AGCAGAACCT 
GAGCTCGACC TCCGCTCAAT TAAGGAAGTC GATGAGGCAA AAGCTAAAGA AGAAAAACTA 
GAGAAGTGTG GTGAGGATGA TGAAACAATC CCATCTGAGT ACAGATTAAA ACCAGCCACG 
GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA 
TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA 
TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG 
GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG 
AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA 
GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA 
GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC 
ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT 
TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA 
GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA 
GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC 
AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT 
GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA 
GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA 
GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA 
CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA 
AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042442.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035907.1 Homo sapiens calpastatin (CAST), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu39731
Clone ID Related Accession (Same CDS sequence)	NM_001042442.3 , XM_006714701.3 , NM_001042442.2
Accession Version	NM_001042442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Homo sapiens(human)
Product	calpastatin isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Oct 22, 2019 this sequence version replaced NM_001042442.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.62328.1, SRR1803613.68618.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035907.1
CDS	48..2357
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042442.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042442.3

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001375317.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362246.1 Homo sapiens calpastatin (CAST), transcript variant 26, mRNA.

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CloneID	OHu39734
Clone ID Related Accession (Same CDS sequence)	XM_006714704.3 , NM_001375317.1
Accession Version	NM_001375317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296919.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT 
GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA 
ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT 
GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT 
ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG 
GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA AAAGGAAGGG 
ATCACAGGGC CTCCTGCAGA CTCTTCGAAA CCCATAGGGC CAGATGATGC TATAGACGCC 
TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG 
GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC 
CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG 
TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA 
GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA 
ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA 
GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA 
TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT 
ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG 
ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC 
CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT 
TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA 
CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA 
GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC 
TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA 
GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC 
CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT 
ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC 
TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT 
AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT 
TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362246.1
CDS	113..2377
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001375317.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375317.1

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT 
GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA 
ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT 
GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT 
ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG 
GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA AAAGGAAGGG 
ATCACAGGGC CTCCTGCAGA CTCTTCGAAA CCCATAGGGC CAGATGATGC TATAGACGCC 
TTGTCATCTG ACTTCACCTG TGGGTCGCCT ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG 
GAATCTACAG AAGTTTTAAA AGCTCAGTCA GCAGGGACAG TCAGAAGTGC TGCTCCACCC 
CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT ACAATGAGTG ATCAAGCACT CGAGGCTCTG 
TCGGCTTCAC TGGGCACCCG GCAAGCAGAA CCTGAGCTCG ACCTCCGCTC AATTAAGGAA 
GTCGATGAGG CAAAAGCTAA AGAAGAAAAA CTAGAGAAGT GTGGTGAGGA TGATGAAACA 
ATCCCATCTG AGTACAGATT AAAACCAGCC ACGGATAAAG ATGGAAAACC ACTATTGCCA 
GAGCCTGAAG AAAAACCCAA GCCTCGGAGT GAATCAGAAC TCATTGATGA ACTTTCAGAA 
GATTTTGACC GGTCTGAATG TAAAGAGAAA CCATCTAAGC CAACTGAAAA GACAGAAGAA 
TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG GAGGCTGTGT GTCGGACCTC CATGTGTAGT 
ATACAGTCAG CACCCCCTGA GCCGGCTACC TTGAAGGGCA CAGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGATAGCCT GGGGAAAAAG GAAGCAGATC CAGAAGATGG AAAACCTGTG 
ATGGATAAAG TCAAGGAGAA GGCCAAAGAA GAAGACCGTG AAAAGCTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAGCCACTC 
CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA CCCATGAGTG AAGACTTCCT TCTGGATGCT 
TTGTCTGAGG ACTTCTCTGG TCCACAAAAT GCTTCATCTC TTAAATTTGA AGATGCTAAA 
CTTGCTGCTG CCATCTCTGA AGTGGTTTCC CAAACCCCAG CTTCAACGAC CCAAGCTGGA 
GCCCCACCCC GTGATACCTC GCAGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC 
TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA 
GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC 
CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT 
ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC 
TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT 
AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT 
TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009920.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865409.1 Homo sapiens calpastatin (CAST), transcript variant X23, mRNA.

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CloneID	OHu96116
Clone ID Related Accession (Same CDS sequence)	XM_017009920.1
Accession Version	XM_017009920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT 
GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA 
ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT 
GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT 
ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG 
GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA ACCCATAGGG 
CCAGATGATG CTATAGACGC CTTGTCATCT GACTTCACCT GTGGGTCGCC TACAGCTGCT 
GGAAAGAAAA CTGAAAAAGA GGAATCTACA GAAGTTTTAA AAGCTCAGTC AGCAGGGACA 
GTCAGAAGTG CTGCTCCACC CCAAGAGAAG AAAAGAAAGG TGGAGAAGGA TACAATGAGT 
GATCAAGCAC TCGAGGCTCT GTCGGCTTCA CTGGGCACCC GGCAAGCAGA ACCTGAGCTC 
GACCTCCGCT CAATTAAGGA AGTCGATGAG GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG 
TGTGGTGAGG ATGATGAAAC AATCCCATCT GAGTACAGAT TAAAACCAGC CACGGATAAA 
GATGGAAAAC CACTATTGCC AGAGCCTGAA GAAAAACCCA AGCCTCGGAG TGAATCAGAA 
CTCATTGATG AACTTTCAGA AGATTTTGAC CGGTCTGAAT GTAAAGAGAA ACCATCTAAG 
CCAACTGAAA AGACAGAAGA ATCTAAGGCC GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG 
TGTCGGACCT CCATGTGTAG TATACAGTCA GCACCCCCTG AGCCGGCTAC CTTGAAGGGC 
ACAGTGCCAG ATGATGCTGT AGAAGCCTTG GCTGATAGCC TGGGGAAAAA GGAAGCAGAT 
CCAGAAGATG GAAAACCTGT GATGGATAAA GTCAAGGAGA AGGCCAAAGA AGAAGACCGT 
GAAAAGCTTG GTGAAAAAGA AGAAACAATT CCTCCTGATT ATAGATTAGA AGAGGTCAAG 
GATAAAGATG GAAAGCCACT CCTGCCAAAA GAGTCTAAGG AACAGCTTCC ACCCATGAGT 
GAAGACTTCC TTCTGGATGC TTTGTCTGAG GACTTCTCTG GTCCACAAAA TGCTTCATCT 
CTTAAATTTG AAGATGCTAA ACTTGCTGCT GCCATCTCTG AAGTGGTTTC CCAAACCCCA 
GCTTCAACGA CCCAAGCTGG AGCCCCACCC CGTGATACCT CGCAGAGTGA CAAAGACCTC 
GATGATGCCT TGGATAAACT CTCTGACAGT CTAGGACAAA GGCAGCCTGA CCCAGATGAG 
AACAAACCAA TGGAAGATAA AGTAAAGGAA AAAGCTAAAG CTGAACATAG AGACAAGCTT 
GGAGAAAGAG ATGACACTAT CCCACCTGAA TACAGACATC TCCTGGATGA TAATGGACAG 
GACAAACCAG TGAAGCCACC TACAAAGAAA TCAGAGGATT CAAAGAAACC TGCAGATGAC 
CAAGACCCCA TTGATGCTCT CTCAGGAGAT CTGGACAGCT GTCCCTCCAC TACAGAAACC 
TCACAGAACA CAGCAAAGGA TAAGTGCAAG AAGGCTGCTT CCAGCTCCAA AGCACCTAAG 
AATGGAGGTA AAGCGAAGGA TTCAGCAAAG ACAACAGAGG AAACTTCCAA GCCAAAAGAT 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865409.1
CDS	112..2337
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009920.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009920.1

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT 
GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA 
ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT 
GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT 
ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG 
GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA ACCCATAGGG 
CCAGATGATG CTATAGACGC CTTGTCATCT GACTTCACCT GTGGGTCGCC TACAGCTGCT 
GGAAAGAAAA CTGAAAAAGA GGAATCTACA GAAGTTTTAA AAGCTCAGTC AGCAGGGACA 
GTCAGAAGTG CTGCTCCACC CCAAGAGAAG AAAAGAAAGG TGGAGAAGGA TACAATGAGT 
GATCAAGCAC TCGAGGCTCT GTCGGCTTCA CTGGGCACCC GGCAAGCAGA ACCTGAGCTC 
GACCTCCGCT CAATTAAGGA AGTCGATGAG GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG 
TGTGGTGAGG ATGATGAAAC AATCCCATCT GAGTACAGAT TAAAACCAGC CACGGATAAA 
GATGGAAAAC CACTATTGCC AGAGCCTGAA GAAAAACCCA AGCCTCGGAG TGAATCAGAA 
CTCATTGATG AACTTTCAGA AGATTTTGAC CGGTCTGAAT GTAAAGAGAA ACCATCTAAG 
CCAACTGAAA AGACAGAAGA ATCTAAGGCC GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG 
TGTCGGACCT CCATGTGTAG TATACAGTCA GCACCCCCTG AGCCGGCTAC CTTGAAGGGC 
ACAGTGCCAG ATGATGCTGT AGAAGCCTTG GCTGATAGCC TGGGGAAAAA GGAAGCAGAT 
CCAGAAGATG GAAAACCTGT GATGGATAAA GTCAAGGAGA AGGCCAAAGA AGAAGACCGT 
GAAAAGCTTG GTGAAAAAGA AGAAACAATT CCTCCTGATT ATAGATTAGA AGAGGTCAAG 
GATAAAGATG GAAAGCCACT CCTGCCAAAA GAGTCTAAGG AACAGCTTCC ACCCATGAGT 
GAAGACTTCC TTCTGGATGC TTTGTCTGAG GACTTCTCTG GTCCACAAAA TGCTTCATCT 
CTTAAATTTG AAGATGCTAA ACTTGCTGCT GCCATCTCTG AAGTGGTTTC CCAAACCCCA 
GCTTCAACGA CCCAAGCTGG AGCCCCACCC CGTGATACCT CGCAGAGTGA CAAAGACCTC 
GATGATGCCT TGGATAAACT CTCTGACAGT CTAGGACAAA GGCAGCCTGA CCCAGATGAG 
AACAAACCAA TGGAAGATAA AGTAAAGGAA AAAGCTAAAG CTGAACATAG AGACAAGCTT 
GGAGAAAGAG ATGACACTAT CCCACCTGAA TACAGACATC TCCTGGATGA TAATGGACAG 
GACAAACCAG TGAAGCCACC TACAAAGAAA TCAGAGGATT CAAAGAAACC TGCAGATGAC 
CAAGACCCCA TTGATGCTCT CTCAGGAGAT CTGGACAGCT GTCCCTCCAC TACAGAAACC 
TCACAGAACA CAGCAAAGGA TAAGTGCAAG AAGGCTGCTT CCAGCTCCAA AGCACCTAAG 
AATGGAGGTA AAGCGAAGGA TTCAGCAAAG ACAACAGAGG AAACTTCCAA GCCAAAAGAT 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009921.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865410.1 Homo sapiens calpastatin (CAST), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu96117
Clone ID Related Accession (Same CDS sequence)	XM_017009921.1
Accession Version	XM_017009921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT 
GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA 
ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT 
GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT 
ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG 
GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA ACCCATAGGG 
CCAGATGATG CTATAGACGC CTTGTCATCT GACTTCACCT GTGGGTCGCC TACAGCTGCT 
GGAAAGAAAA CTGAAAAAGA GGAATCTACA GAAGTTTTAA AAGCTCAGTC AGCAGGGACA 
GTCAGAAGTG CTGCTCCACC CCAAGAGAAG AAAAGAAAGG TGGAGAAGGA TACAATGAGT 
GATCAAGCAC TCGAGGCTCT GTCGGCTTCA CTGGGCACCC GGCAAGCAGA ACCTGAGCTC 
GACCTCCGCT CAATTAAGGA AGTCGATGAG GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG 
TGTGGTGAGG ATGATGAAAC AATCCCATCT GAGTACAGAT TAAAACCAGC CACGGATAAA 
GATGGAAAAC CACTATTGCC AGAGCCTGAA GAAAAACCCA AGCCTCGGAG TGAATCAGAA 
CTCATTGATG AACTTTCAGA AGATTTTGAC CGGTCTGAAT GTAAAGAGAA ACCATCTAAG 
CCAACTGAAA AGACAGAAGA ATCTAAGGCC GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG 
TGTCGGACCT CCATGTGTAG TATACAGTCA GCACCCCCTG AGCCGGCTAC CTTGAAGGGC 
ACAGTGCCAG ATGATGCTGT AGAAGCCTTG GCTGATAGCC TGGGGAAAAA GGAAGCAGAT 
CCAGAAGATG GAAAACCTGT GATGGATAAA GTCAAGGAGA AGGCCAAAGA AGAAGACCGT 
GAAAAGCTTG GTGAAAAAGA AGAAACAATT CCTCCTGATT ATAGATTAGA AGAGGTCAAG 
GATAAAGATG GAAAGCCACT CCTGCCAAAA GAGTCTAAGG AACAGCTTCC ACCCATGAGT 
GAAGACTTCC TTCTGGATGC TTTGTCTGAG GACTTCTCTG GTCCACAAAA TGCTTCATCT 
CTTAAATTTG AAGATGCTAA ACTTGCTGCT GCCATCTCTG AAGTGGTTTC CCAAACCCCA 
GCTTCAACGA CCCAAGCTGG AGCCCCACCC CGTGATACCT CGAGTGACAA AGACCTCGAT 
GATGCCTTGG ATAAACTCTC TGACAGTCTA GGACAAAGGC AGCCTGACCC AGATGAGAAC 
AAACCAATGG AAGATAAAGT AAAGGAAAAA GCTAAAGCTG AACATAGAGA CAAGCTTGGA 
GAAAGAGATG ACACTATCCC ACCTGAATAC AGACATCTCC TGGATGATAA TGGACAGGAC 
AAACCAGTGA AGCCACCTAC AAAGAAATCA GAGGATTCAA AGAAACCTGC AGATGACCAA 
GACCCCATTG ATGCTCTCTC AGGAGATCTG GACAGCTGTC CCTCCACTAC AGAAACCTCA 
CAGAACACAG CAAAGGATAA GTGCAAGAAG GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT 
GGAGGTAAAG CGAAGGATTC AGCAAAGACA ACAGAGGAAA CTTCCAAGCC AAAAGATGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865410.1
CDS	129..2351
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009921.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009921.1

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCTG TCTGTGGTTC ATGAGAAAAA ATCCCAAGAA 
GGAAAGCCAA AAGAACACAC AGAGCCAAAA AGCCTACCCA AGCAGGCATC AGATACAGGA 
AGTAACGATG CTCACAATAA AAAAGCAGTT TCCAGATCAG CTGAACAGCA GCCATCAGAG 
AAATCAACAG AACCAAAGAC TAAACCACAA GACATGATTT CTGCTGGTGG AGAGAGTGTT 
GCTGGTATCA CTGCAATATC TGGCAAGCCG GGTGACAAGA AAAAAGAAAA GAAATCATTA 
ACCCCAGCTG TGCCAGTTGA ATCTAAACCG GATAAACCAT CGGGAAAGTC AGGCATGGAT 
GCTGCTTTGG ATGACTTAAT AGATACTTTA GGAGGACCTG AAGAAACTGA AGAAGAAAAT 
ACAACGTATA CTGGACCAGA AGTTTCAGAT CCAATGAGTT CCACCTACAT AGAGGAATTG 
GGTAAAAGAG AAGTCACAAT TCCTCCAAAA TATAGGGAAC TATTGGCTAA ACCCATAGGG 
CCAGATGATG CTATAGACGC CTTGTCATCT GACTTCACCT GTGGGTCGCC TACAGCTGCT 
GGAAAGAAAA CTGAAAAAGA GGAATCTACA GAAGTTTTAA AAGCTCAGTC AGCAGGGACA 
GTCAGAAGTG CTGCTCCACC CCAAGAGAAG AAAAGAAAGG TGGAGAAGGA TACAATGAGT 
GATCAAGCAC TCGAGGCTCT GTCGGCTTCA CTGGGCACCC GGCAAGCAGA ACCTGAGCTC 
GACCTCCGCT CAATTAAGGA AGTCGATGAG GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG 
TGTGGTGAGG ATGATGAAAC AATCCCATCT GAGTACAGAT TAAAACCAGC CACGGATAAA 
GATGGAAAAC CACTATTGCC AGAGCCTGAA GAAAAACCCA AGCCTCGGAG TGAATCAGAA 
CTCATTGATG AACTTTCAGA AGATTTTGAC CGGTCTGAAT GTAAAGAGAA ACCATCTAAG 
CCAACTGAAA AGACAGAAGA ATCTAAGGCC GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG 
TGTCGGACCT CCATGTGTAG TATACAGTCA GCACCCCCTG AGCCGGCTAC CTTGAAGGGC 
ACAGTGCCAG ATGATGCTGT AGAAGCCTTG GCTGATAGCC TGGGGAAAAA GGAAGCAGAT 
CCAGAAGATG GAAAACCTGT GATGGATAAA GTCAAGGAGA AGGCCAAAGA AGAAGACCGT 
GAAAAGCTTG GTGAAAAAGA AGAAACAATT CCTCCTGATT ATAGATTAGA AGAGGTCAAG 
GATAAAGATG GAAAGCCACT CCTGCCAAAA GAGTCTAAGG AACAGCTTCC ACCCATGAGT 
GAAGACTTCC TTCTGGATGC TTTGTCTGAG GACTTCTCTG GTCCACAAAA TGCTTCATCT 
CTTAAATTTG AAGATGCTAA ACTTGCTGCT GCCATCTCTG AAGTGGTTTC CCAAACCCCA 
GCTTCAACGA CCCAAGCTGG AGCCCCACCC CGTGATACCT CGAGTGACAA AGACCTCGAT 
GATGCCTTGG ATAAACTCTC TGACAGTCTA GGACAAAGGC AGCCTGACCC AGATGAGAAC 
AAACCAATGG AAGATAAAGT AAAGGAAAAA GCTAAAGCTG AACATAGAGA CAAGCTTGGA 
GAAAGAGATG ACACTATCCC ACCTGAATAC AGACATCTCC TGGATGATAA TGGACAGGAC 
AAACCAGTGA AGCCACCTAC AAAGAAATCA GAGGATTCAA AGAAACCTGC AGATGACCAA 
GACCCCATTG ATGCTCTCTC AGGAGATCTG GACAGCTGTC CCTCCACTAC AGAAACCTCA 
CAGAACACAG CAAAGGATAA GTGCAAGAAG GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT 
GGAGGTAAAG CGAAGGATTC AGCAAAGACA ACAGAGGAAA CTTCCAAGCC AAAAGATGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009925.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865414.1 Homo sapiens calpastatin (CAST), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OHu96121
Clone ID Related Accession (Same CDS sequence)	XM_017009925.1
Accession Version	XM_017009925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA 
GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA 
GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT 
GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA 
TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG 
GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA 
AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA 
TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAACCCATA 
GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT 
GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG 
ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG 
AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG 
CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG 
AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT 
AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA 
GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT 
AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT 
GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG 
GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA 
GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC 
CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC 
AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG 
AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA 
TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC 
CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGCAGAG TGACAAAGAC 
CTCGATGATG CCTTGGATAA ACTCTCTGAC AGTCTAGGAC AAAGGCAGCC TGACCCAGAT 
GAGAACAAAC CAATGGAAGA TAAAGTAAAG GAAAAAGCTA AAGCTGAACA TAGAGACAAG 
CTTGGAGAAA GAGATGACAC TATCCCACCT GAATACAGAC ATCTCCTGGA TGATAATGGA 
CAGGACAAAC CAGTGAAGCC ACCTACAAAG AAATCAGAGG ATTCAAAGAA ACCTGCAGAT 
GACCAAGACC CCATTGATGC TCTCTCAGGA GATCTGGACA GCTGTCCCTC CACTACAGAA 
ACCTCACAGA ACACAGCAAA GGATAAGTGC AAGAAGGCTG CTTCCAGCTC CAAAGCACCT 
AAGAATGGAG GTAAAGCGAA GGATTCAGCA AAGACAACAG AGGAAACTTC CAAGCCAAAA 
GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865414.1
CDS	138..2306
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009925.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009925.1

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA 
GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA 
GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT 
GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA 
TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG 
GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA 
AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA 
TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAACCCATA 
GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT 
GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG 
ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG 
AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG 
CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG 
AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT 
AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA 
GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT 
AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT 
GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG 
GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA 
GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC 
CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC 
AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG 
AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA 
TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC 
CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGCAGAG TGACAAAGAC 
CTCGATGATG CCTTGGATAA ACTCTCTGAC AGTCTAGGAC AAAGGCAGCC TGACCCAGAT 
GAGAACAAAC CAATGGAAGA TAAAGTAAAG GAAAAAGCTA AAGCTGAACA TAGAGACAAG 
CTTGGAGAAA GAGATGACAC TATCCCACCT GAATACAGAC ATCTCCTGGA TGATAATGGA 
CAGGACAAAC CAGTGAAGCC ACCTACAAAG AAATCAGAGG ATTCAAAGAA ACCTGCAGAT 
GACCAAGACC CCATTGATGC TCTCTCAGGA GATCTGGACA GCTGTCCCTC CACTACAGAA 
ACCTCACAGA ACACAGCAAA GGATAAGTGC AAGAAGGCTG CTTCCAGCTC CAAAGCACCT 
AAGAATGGAG GTAAAGCGAA GGATTCAGCA AAGACAACAG AGGAAACTTC CAAGCCAAAA 
GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009926.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865415.1 Homo sapiens calpastatin (CAST), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OHu96122
Clone ID Related Accession (Same CDS sequence)	XM_017009926.1
Accession Version	XM_017009926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA 
GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA 
GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT 
GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA 
TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG 
GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA 
AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA 
TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAACCCATA 
GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT 
GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG 
ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG 
AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG 
CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG 
AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT 
AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA 
GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT 
AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT 
GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG 
GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA 
GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC 
CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC 
AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG 
AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA 
TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC 
CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGAGTGA CAAAGACCTC 
GATGATGCCT TGGATAAACT CTCTGACAGT CTAGGACAAA GGCAGCCTGA CCCAGATGAG 
AACAAACCAA TGGAAGATAA AGTAAAGGAA AAAGCTAAAG CTGAACATAG AGACAAGCTT 
GGAGAAAGAG ATGACACTAT CCCACCTGAA TACAGACATC TCCTGGATGA TAATGGACAG 
GACAAACCAG TGAAGCCACC TACAAAGAAA TCAGAGGATT CAAAGAAACC TGCAGATGAC 
CAAGACCCCA TTGATGCTCT CTCAGGAGAT CTGGACAGCT GTCCCTCCAC TACAGAAACC 
TCACAGAACA CAGCAAAGGA TAAGTGCAAG AAGGCTGCTT CCAGCTCCAA AGCACCTAAG 
AATGGAGGTA AAGCGAAGGA TTCAGCAAAG ACAACAGAGG AAACTTCCAA GCCAAAAGAT 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865415.1
CDS	138..2303
Translation	MAFASWWYKTHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009926.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009926.1

ORF Insert Sequence:

ATGGCATTCG CCAGCTGGTG GTACAAGACG CATGTCAGTG AAAAAACCAG TGAATCGCCT 
TCCAAACCAG GAGAAAAGAA AGGATCAGAT GAGAAAAAAG CAGCAAGCCT CGGCAGCAGT 
CAATCCTCCA GAACCTATGC TGGTGGAACA GCCTCGGCCA CCAAGGTGTC AGCTTCCTCT 
GGTGCAACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTGT AAAAACAGAA 
CCTGAGAAGA AGTCACAGTC AACCAAGCCA AAAAGCCTAC CCAAGCAGGC ATCAGATACA 
GGAAGTAACG ATGCTCACAA TAAAAAAGCA GTTTCCAGAT CAGCTGAACA GCAGCCATCA 
GAGAAATCAA CAGAACCAAA GACTAAACCA CAAGACATGA TTTCTGCTGG TGGAGAGAGT 
GTTGCTGGTA TCACTGCAAT ATCTGGCAAG CCGGGTGACA AGAAAAAAGA AAAGAAATCA 
TTAACCCCAG CTGTGCCAGT TGAATCTAAA CCGGATAAAC CATCGGGAAA GTCAGGCATG 
GATGCTGCTT TGGATGACTT AATAGATACT TTAGGAGGAC CTGAAGAAAC TGAAGAAGAA 
AATACAACGT ATACTGGACC AGAAGTTTCA GATCCAATGA GTTCCACCTA CATAGAGGAA 
TTGGGTAAAA GAGAAGTCAC AATTCCTCCA AAATATAGGG AACTATTGGC TAAACCCATA 
GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT 
GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG 
ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG 
AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG 
CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG 
AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT 
AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA 
GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT 
AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT 
GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG 
GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA 
GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC 
CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC 
AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG 
AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA 
TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC 
CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGAGTGA CAAAGACCTC 
GATGATGCCT TGGATAAACT CTCTGACAGT CTAGGACAAA GGCAGCCTGA CCCAGATGAG 
AACAAACCAA TGGAAGATAA AGTAAAGGAA AAAGCTAAAG CTGAACATAG AGACAAGCTT 
GGAGAAAGAG ATGACACTAT CCCACCTGAA TACAGACATC TCCTGGATGA TAATGGACAG 
GACAAACCAG TGAAGCCACC TACAAAGAAA TCAGAGGATT CAAAGAAACC TGCAGATGAC 
CAAGACCCCA TTGATGCTCT CTCAGGAGAT CTGGACAGCT GTCCCTCCAC TACAGAAACC 
TCACAGAACA CAGCAAAGGA TAAGTGCAAG AAGGCTGCTT CCAGCTCCAA AGCACCTAAG 
AATGGAGGTA AAGCGAAGGA TTCAGCAAAG ACAACAGAGG AAACTTCCAA GCCAAAAGAT 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009927.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865416.1 Homo sapiens calpastatin (CAST), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OHu96123
Clone ID Related Accession (Same CDS sequence)	XM_017009927.1
Accession Version	XM_017009927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAAAAGGAAG GGATCACAGG GCCTCCTGCA 
GACTCTTCGA AACCCATAGG GCCAGATGAT GCTATAGACG CCTTGTCATC TGACTTCACC 
TGTGGGTCGC CTACAGCTGC TGGAAAGAAA ACTGAAAAAG AGGAATCTAC AGAAGTTTTA 
AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG 
GTGGAGAAGG ATACAATGAG TGATCAAGCA CTCGAGGCTC TGTCGGCTTC ACTGGGCACC 
CGGCAAGCAG AACCTGAGCT CGACCTCCGC TCAATTAAGG AAGTCGATGA GGCAAAAGCT 
AAAGAAGAAA AACTAGAGAA GTGTGGTGAG GATGATGAAA CAATCCCATC TGAGTACAGA 
TTAAAACCAG CCACGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAAAACCC 
AAGCCTCGGA GTGAATCAGA ACTCATTGAT GAACTTTCAG AAGATTTTGA CCGGTCTGAA 
TGTAAAGAGA AACCATCTAA GCCAACTGAA AAGACAGAAG AATCTAAGGC CGCTGCTCCA 
GCTCCTGTGT CGGAGGCTGT GTGTCGGACC TCCATGTGTA GTATACAGTC AGCACCCCCT 
GAGCCGGCTA CCTTGAAGGG CACAGTGCCA GATGATGCTG TAGAAGCCTT GGCTGATAGC 
CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT GGAAAACCTG TGATGGATAA AGTCAAGGAG 
AAGGCCAAAG AAGAAGACCG TGAAAAGCTT GGTGAAAAAG AAGAAACAAT TCCTCCTGAT 
TATAGATTAG AAGAGGTCAA GGATAAAGAT GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG 
GAACAGCTTC CACCCATGAG TGAAGACTTC CTTCTGGATG CTTTGTCTGA GGACTTCTCT 
GGTCCACAAA ATGCTTCATC TCTTAAATTT GAAGATGCTA AACTTGCTGC TGCCATCTCT 
GAAGTGGTTT CCCAAACCCC AGCTTCAACG ACCCAAGCTG GAGCCCCACC CCGTGATACC 
TCGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG 
CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT 
GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC 
CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA 
AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT 
CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC 
AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA 
ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865416.1
CDS	169..2292
Translation	MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009927.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009927.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAAAAGGAAG GGATCACAGG GCCTCCTGCA 
GACTCTTCGA AACCCATAGG GCCAGATGAT GCTATAGACG CCTTGTCATC TGACTTCACC 
TGTGGGTCGC CTACAGCTGC TGGAAAGAAA ACTGAAAAAG AGGAATCTAC AGAAGTTTTA 
AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG 
GTGGAGAAGG ATACAATGAG TGATCAAGCA CTCGAGGCTC TGTCGGCTTC ACTGGGCACC 
CGGCAAGCAG AACCTGAGCT CGACCTCCGC TCAATTAAGG AAGTCGATGA GGCAAAAGCT 
AAAGAAGAAA AACTAGAGAA GTGTGGTGAG GATGATGAAA CAATCCCATC TGAGTACAGA 
TTAAAACCAG CCACGGATAA AGATGGAAAA CCACTATTGC CAGAGCCTGA AGAAAAACCC 
AAGCCTCGGA GTGAATCAGA ACTCATTGAT GAACTTTCAG AAGATTTTGA CCGGTCTGAA 
TGTAAAGAGA AACCATCTAA GCCAACTGAA AAGACAGAAG AATCTAAGGC CGCTGCTCCA 
GCTCCTGTGT CGGAGGCTGT GTGTCGGACC TCCATGTGTA GTATACAGTC AGCACCCCCT 
GAGCCGGCTA CCTTGAAGGG CACAGTGCCA GATGATGCTG TAGAAGCCTT GGCTGATAGC 
CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT GGAAAACCTG TGATGGATAA AGTCAAGGAG 
AAGGCCAAAG AAGAAGACCG TGAAAAGCTT GGTGAAAAAG AAGAAACAAT TCCTCCTGAT 
TATAGATTAG AAGAGGTCAA GGATAAAGAT GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG 
GAACAGCTTC CACCCATGAG TGAAGACTTC CTTCTGGATG CTTTGTCTGA GGACTTCTCT 
GGTCCACAAA ATGCTTCATC TCTTAAATTT GAAGATGCTA AACTTGCTGC TGCCATCTCT 
GAAGTGGTTT CCCAAACCCC AGCTTCAACG ACCCAAGCTG GAGCCCCACC CCGTGATACC 
TCGAGTGACA AAGACCTCGA TGATGCCTTG GATAAACTCT CTGACAGTCT AGGACAAAGG 
CAGCCTGACC CAGATGAGAA CAAACCAATG GAAGATAAAG TAAAGGAAAA AGCTAAAGCT 
GAACATAGAG ACAAGCTTGG AGAAAGAGAT GACACTATCC CACCTGAATA CAGACATCTC 
CTGGATGATA ATGGACAGGA CAAACCAGTG AAGCCACCTA CAAAGAAATC AGAGGATTCA 
AAGAAACCTG CAGATGACCA AGACCCCATT GATGCTCTCT CAGGAGATCT GGACAGCTGT 
CCCTCCACTA CAGAAACCTC ACAGAACACA GCAAAGGATA AGTGCAAGAA GGCTGCTTCC 
AGCTCCAAAG CACCTAAGAA TGGAGGTAAA GCGAAGGATT CAGCAAAGAC AACAGAGGAA 
ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009928.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865417.1 Homo sapiens calpastatin (CAST), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OHu96124
Clone ID Related Accession (Same CDS sequence)	XM_017009928.1
Accession Version	XM_017009928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC 
TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA 
GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC 
CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT 
ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC 
TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT 
AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT 
TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865417.1
CDS	207..2291
Translation	MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009928.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009928.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCCATTCCA GTCAGCCAAC AGATGGAAGG ACCACATCTT 
CCTAACAAGA AAAAACACAA AAAACAGGCT GTAAAAACAG AACCTGAGAA GAAGTCACAG 
TCAACCAAGC TGTCTGTGGT TCATGAGAAA AAATCCCAAG AAGGAAAGCC AAAAGAACAC 
ACAGAGCCAA AAAGCCTACC CAAGCAGGCA TCAGATACAG GAAGTAACGA TGCTCACAAT 
AAAAAAGCAG TTTCCAGATC AGCTGAACAG CAGCCATCAG AGAAATCAAC AGAACCAAAG 
ACTAAACCAC AAGACATGAT TTCTGCTGGT GGAGAGAGTG TTGCTGGTAT CACTGCAATA 
TCTGGCAAGC CGGGTGACAA GAAAAAAGAA AAGAAATCAT TAACCCCAGC TGTGCCAGTT 
GAATCTAAAC CGGATAAACC ATCGGGAAAG TCAGGCATGG ATGCTGCTTT GGATGACTTA 
ATAGATACTT TAGGAGGACC TGAAGAAACT GAAGAAGAAA ATACAACGTA TACTGGACCA 
GAAGTTTCAG ATCCAATGAG TTCCACCTAC ATAGAGGAAT TGGGTAAAAG AGAAGTCACA 
ATTCCTCCAA AATATAGGGA ACTATTGGCT AAACCCATAG GGCCAGATGA TGCTATAGAC 
GCCTTGTCAT CTGACTTCAC CTGTGGGTCG CCTACAGCTG CTGGAAAGAA AACTGAAAAA 
GAGGAATCTA CAGAAGTTTT AAAAGCTCAG TCAGCAGGGA CAGTCAGAAG TGCTGCTCCA 
CCCCAAGAGA AGAAAAGAAA GGTGGAGAAG GATACAATGA GTGATCAAGC ACTCGAGGCT 
CTGTCGGCTT CACTGGGCAC CCGGCAAGCA GAACCTGAGC TCGACCTCCG CTCAATTAAG 
GAAGTCGATG AGGCAAAAGC TAAAGAAGAA AAACTAGAGA AGTGTGGTGA GGATGATGAA 
ACAATCCCAT CTGAGTACAG ATTAAAACCA GCCACGGATA AAGATGGAAA ACCACTATTG 
CCAGAGCCTG AAGAAAAACC CAAGCCTCGG AGTGAATCAG AACTCATTGA TGAACTTTCA 
GAAGATTTTG ACCGGTCTGA ATGTAAAGAG AAACCATCTA AGCCAACTGA AAAGACAGAA 
GAATCTAAGG CCGCTGCTCC AGCTCCTGTG TCGGAGGCTG TGTGTCGGAC CTCCATGTGT 
AGTATACAGT CAGCACCCCC TGAGCCGGCT ACCTTGAAGG GCACAGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGATAG CCTGGGGAAA AAGGAAGCAG ATCCAGAAGA TGGAAAACCT 
GTGATGGATA AAGTCAAGGA GAAGGCCAAA GAAGAAGACC GTGAAAAGCT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAGCCA 
CTCCTGCCAA AAGAGTCTAA GGAACAGCTT CCACCCATGA GTGAAGACTT CCTTCTGGAT 
GCTTTGTCTG AGGACTTCTC TGGTCCACAA AATGCTTCAT CTCTTAAATT TGAAGATGCT 
AAACTTGCTG CTGCCATCTC TGAAGTGGTT TCCCAAACCC CAGCTTCAAC GACCCAAGCT 
GGAGCCCCAC CCCGTGATAC CTCGAGTGAC AAAGACCTCG ATGATGCCTT GGATAAACTC 
TCTGACAGTC TAGGACAAAG GCAGCCTGAC CCAGATGAGA ACAAACCAAT GGAAGATAAA 
GTAAAGGAAA AAGCTAAAGC TGAACATAGA GACAAGCTTG GAGAAAGAGA TGACACTATC 
CCACCTGAAT ACAGACATCT CCTGGATGAT AATGGACAGG ACAAACCAGT GAAGCCACCT 
ACAAAGAAAT CAGAGGATTC AAAGAAACCT GCAGATGACC AAGACCCCAT TGATGCTCTC 
TCAGGAGATC TGGACAGCTG TCCCTCCACT ACAGAAACCT CACAGAACAC AGCAAAGGAT 
AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA GCACCTAAGA ATGGAGGTAA AGCGAAGGAT 
TCAGCAAAGA CAACAGAGGA AACTTCCAAG CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009931.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865420.1 Homo sapiens calpastatin (CAST), transcript variant X40, mRNA.

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CloneID	OHu96125
Clone ID Related Accession (Same CDS sequence)	XM_017009931.1
Accession Version	XM_017009931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAACCC ATAGGGCCAG ATGATGCTAT AGACGCCTTG 
TCATCTGACT TCACCTGTGG GTCGCCTACA GCTGCTGGAA AGAAAACTGA AAAAGAGGAA 
TCTACAGAAG TTTTAAAAGC TCAGTCAGCA GGGACAGTCA GAAGTGCTGC TCCACCCCAA 
GAGAAGAAAA GAAAGGTGGA GAAGGATACA ATGAGTGATC AAGCACTCGA GGCTCTGTCG 
GCTTCACTGG GCACCCGGCA AGCAGAACCT GAGCTCGACC TCCGCTCAAT TAAGGAAGTC 
GATGAGGCAA AAGCTAAAGA AGAAAAACTA GAGAAGTGTG GTGAGGATGA TGAAACAATC 
CCATCTGAGT ACAGATTAAA ACCAGCCACG GATAAAGATG GAAAACCACT ATTGCCAGAG 
CCTGAAGAAA AACCCAAGCC TCGGAGTGAA TCAGAACTCA TTGATGAACT TTCAGAAGAT 
TTTGACCGGT CTGAATGTAA AGAGAAACCA TCTAAGCCAA CTGAAAAGAC AGAAGAATCT 
AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG GCTGTGTGTC GGACCTCCAT GTGTAGTATA 
CAGTCAGCAC CCCCTGAGCC GGCTACCTTG AAGGGCACAG TGCCAGATGA TGCTGTAGAA 
GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA GCAGATCCAG AAGATGGAAA ACCTGTGATG 
GATAAAGTCA AGGAGAAGGC CAAAGAAGAA GACCGTGAAA AGCTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA GCCACTCCTG 
CCAAAAGAGT CTAAGGAACA GCTTCCACCC ATGAGTGAAG ACTTCCTTCT GGATGCTTTG 
TCTGAGGACT TCTCTGGTCC ACAAAATGCT TCATCTCTTA AATTTGAAGA TGCTAAACTT 
GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTT CAACGACCCA AGCTGGAGCC 
CCACCCCGTG ATACCTCGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC 
AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG 
GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT 
GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG 
AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA 
GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC 
AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA 
AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865420.1
CDS	153..2171
Translation	MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009931.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009931.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGTAAAA ACAGAACCTG AGAAGAAGTC ACAGTCAACC 
AAGCTGTCTG TGGTTCATGA GAAAAAATCC CAAGAAGGAA AGCCAAAAGA ACACACAGAG 
CCAAAAAGCC TACCCAAGCA GGCATCAGAT ACAGGAAGTA ACGATGCTCA CAATAAAAAA 
GCAGTTTCCA GATCAGCTGA ACAGCAGCCA TCAGAGAAAT CAACAGAACC AAAGACTAAA 
CCACAAGACA TGATTTCTGC TGGTGGAGAG AGTGTTGCTG GTATCACTGC AATATCTGGC 
AAGCCGGGTG ACAAGAAAAA AGAAAAGAAA TCATTAACCC CAGCTGTGCC AGTTGAATCT 
AAACCGGATA AACCATCGGG AAAGTCAGGC ATGGATGCTG CTTTGGATGA CTTAATAGAT 
ACTTTAGGAG GACCTGAAGA AACTGAAGAA GAAAATACAA CGTATACTGG ACCAGAAGTT 
TCAGATCCAA TGAGTTCCAC CTACATAGAG GAATTGGGTA AAAGAGAAGT CACAATTCCT 
CCAAAATATA GGGAACTATT GGCTAAACCC ATAGGGCCAG ATGATGCTAT AGACGCCTTG 
TCATCTGACT TCACCTGTGG GTCGCCTACA GCTGCTGGAA AGAAAACTGA AAAAGAGGAA 
TCTACAGAAG TTTTAAAAGC TCAGTCAGCA GGGACAGTCA GAAGTGCTGC TCCACCCCAA 
GAGAAGAAAA GAAAGGTGGA GAAGGATACA ATGAGTGATC AAGCACTCGA GGCTCTGTCG 
GCTTCACTGG GCACCCGGCA AGCAGAACCT GAGCTCGACC TCCGCTCAAT TAAGGAAGTC 
GATGAGGCAA AAGCTAAAGA AGAAAAACTA GAGAAGTGTG GTGAGGATGA TGAAACAATC 
CCATCTGAGT ACAGATTAAA ACCAGCCACG GATAAAGATG GAAAACCACT ATTGCCAGAG 
CCTGAAGAAA AACCCAAGCC TCGGAGTGAA TCAGAACTCA TTGATGAACT TTCAGAAGAT 
TTTGACCGGT CTGAATGTAA AGAGAAACCA TCTAAGCCAA CTGAAAAGAC AGAAGAATCT 
AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG GCTGTGTGTC GGACCTCCAT GTGTAGTATA 
CAGTCAGCAC CCCCTGAGCC GGCTACCTTG AAGGGCACAG TGCCAGATGA TGCTGTAGAA 
GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA GCAGATCCAG AAGATGGAAA ACCTGTGATG 
GATAAAGTCA AGGAGAAGGC CAAAGAAGAA GACCGTGAAA AGCTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA GCCACTCCTG 
CCAAAAGAGT CTAAGGAACA GCTTCCACCC ATGAGTGAAG ACTTCCTTCT GGATGCTTTG 
TCTGAGGACT TCTCTGGTCC ACAAAATGCT TCATCTCTTA AATTTGAAGA TGCTAAACTT 
GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTT CAACGACCCA AGCTGGAGCC 
CCACCCCGTG ATACCTCGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC 
AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG 
GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT 
GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG 
AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA 
GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC 
AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA 
AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330626.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317555.1 Homo sapiens calpastatin (CAST), transcript variant 17, mRNA.

Price & Availability↑

CloneID	OHu102305
Clone ID Related Accession (Same CDS sequence)	NM_001330626.2 , NM_001330626.1
Accession Version	NM_001330626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	calpastatin isoform p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001330626.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.3952.1, SRR1660805.145931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT 
GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG 
AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA 
AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA 
GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC 
CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT 
GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA 
AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT 
GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT 
GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG 
ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA 
GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC 
TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA 
TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA 
ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT 
GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA 
CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA 
AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA 
CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC 
CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG 
AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA 
GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317555.1
CDS	48..2327
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330626.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330626.2

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT 
GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG 
AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA 
AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA 
GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC 
CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT 
GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA 
AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT 
GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT 
GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG 
ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA 
GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC 
TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA 
TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA 
ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT 
GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA 
CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA 
AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA 
CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC 
CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG 
AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA 
GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330626.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317555.1 Homo sapiens calpastatin (CAST), transcript variant 17, mRNA.

Price & Availability↑

CloneID	OHu102305
Clone ID Related Accession (Same CDS sequence)	NM_001330626.2 , NM_001330626.1
Accession Version	NM_001330626.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	calpastatin isoform p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_017009918.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.3952.1, SRR1660805.145931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT 
GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG 
AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA 
AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA 
GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC 
CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT 
GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA 
AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT 
GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT 
GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG 
ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA 
GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC 
TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA 
TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA 
ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT 
GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA 
CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA 
AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA 
CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC 
CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG 
AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA 
GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317555.1
CDS	267..2546
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330626.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330626.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT 
GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG 
AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA 
AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA 
GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC 
CGCTCAATTA AGGAAGTCGA TGAGGCAAAA GCTAAAGAAG AAAAACTAGA GAAGTGTGGT 
GAGGATGATG AAACAATCCC ATCTGAGTAC AGATTAAAAC CAGCCACGGA TAAAGATGGA 
AAACCACTAT TGCCAGAGCC TGAAGAAAAA CCCAAGCCTC GGAGTGAATC AGAACTCATT 
GATGAACTTT CAGAAGATTT TGACCGGTCT GAATGTAAAG AGAAACCATC TAAGCCAACT 
GAAAAGACAG AAGAATCTAA GGCCGCTGCT CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG 
ACCTCCATGT GTAGTATACA GTCAGCACCC CCTGAGCCGG CTACCTTGAA GGGCACAGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGAT AGCCTGGGGA AAAAGGAAGC AGATCCAGAA 
GATGGAAAAC CTGTGATGGA TAAAGTCAAG GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAGC CACTCCTGCC AAAAGAGTCT AAGGAACAGC TTCCACCCAT GAGTGAAGAC 
TTCCTTCTGG ATGCTTTGTC TGAGGACTTC TCTGGTCCAC AAAATGCTTC ATCTCTTAAA 
TTTGAAGATG CTAAACTTGC TGCTGCCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA 
ACGACCCAAG CTGGAGCCCC ACCCCGTGAT ACCTCGCAGA GTGACAAAGA CCTCGATGAT 
GCCTTGGATA AACTCTCTGA CAGTCTAGGA CAAAGGCAGC CTGACCCAGA TGAGAACAAA 
CCAATGGAAG ATAAAGTAAA GGAAAAAGCT AAAGCTGAAC ATAGAGACAA GCTTGGAGAA 
AGAGATGACA CTATCCCACC TGAATACAGA CATCTCCTGG ATGATAATGG ACAGGACAAA 
CCAGTGAAGC CACCTACAAA GAAATCAGAG GATTCAAAGA AACCTGCAGA TGACCAAGAC 
CCCATTGATG CTCTCTCAGG AGATCTGGAC AGCTGTCCCT CCACTACAGA AACCTCACAG 
AACACAGCAA AGGATAAGTG CAAGAAGGCT GCTTCCAGCT CCAAAGCACC TAAGAATGGA 
GGTAAAGCGA AGGATTCAGC AAAGACAACA GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001750.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001741.4 Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu39726
Clone ID Related Accession (Same CDS sequence)	NM_001750.6 , XM_006714696.3 , NM_001750.7
Accession Version	NM_001750.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	calpastatin isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced NM_001750.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.113174.1, SRR1660803.78729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001741.4
CDS	267..2642
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001750.6
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001750.6

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001750.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_001741.4 Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu39726
Clone ID Related Accession (Same CDS sequence)	NM_001750.6 , XM_006714696.3 , NM_001750.7
Accession Version	NM_001750.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Homo sapiens(human)
Product	calpastatin isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001750.6. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.113174.1, SRR1660803.78729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001741.4
CDS	48..2423
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001750.7
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001750.7

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042441.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035906.1 Homo sapiens calpastatin (CAST), transcript variant 7, mRNA.

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CloneID	OHu39730
Clone ID Related Accession (Same CDS sequence)	NM_001042441.3 , XM_017009913.1 , NM_001042441.2
Accession Version	NM_001042441.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	calpastatin isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001042441.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.137982.1, SRR1660805.114181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA 
GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA 
TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT 
ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG 
AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT 
TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT 
GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA 
TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT 
GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT 
GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG 
GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG 
TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC 
TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT 
AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA 
AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT 
GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT 
GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA 
CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC 
AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG 
GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT 
GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG 
AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA 
GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC 
AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA 
AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035906.1
CDS	48..2366
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042441.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042441.3

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA 
GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA 
TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT 
ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG 
AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT 
TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT 
GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA 
TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT 
GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT 
GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG 
GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG 
TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC 
TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT 
AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA 
AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT 
GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT 
GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA 
CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC 
AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG 
GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT 
GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG 
AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA 
GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC 
AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA 
AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042441.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035906.1 Homo sapiens calpastatin (CAST), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu39730
Clone ID Related Accession (Same CDS sequence)	NM_001042441.3 , XM_017009913.1 , NM_001042441.2
Accession Version	NM_001042441.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	calpastatin isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 30, 2016 this sequence version replaced XM_006714700.3, NM_001042441.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.114181.1, SRR1803612.137982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA 
GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA 
TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT 
ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG 
AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT 
TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT 
GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA 
TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT 
GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT 
GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG 
GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG 
TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC 
TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT 
AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA 
AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT 
GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT 
GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA 
CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC 
AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG 
GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT 
GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG 
AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA 
GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC 
AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA 
AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035906.1
CDS	267..2585
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042441.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042441.2

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA 
GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA 
TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT 
ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG 
AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT 
TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT 
GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA 
TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT 
GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT 
GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG 
GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG 
TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC 
TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT 
AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA 
AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT 
GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT 
GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA 
CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC 
AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG 
GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT 
GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG 
AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA 
GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC 
AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA 
AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001042442.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035907.1 Homo sapiens calpastatin (CAST), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu39731
Clone ID Related Accession (Same CDS sequence)	NM_001042442.3 , XM_006714701.3 , NM_001042442.2
Accession Version	NM_001042442.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	calpastatin isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On or before Aug 10, 2016 this sequence version replaced NM_001329966.1, NM_001042442.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.163156.1, SRR1660807.241791.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035907.1
CDS	201..2510
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001042442.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042442.2

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330627.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317556.1 Homo sapiens calpastatin (CAST), transcript variant 18, mRNA.

Price & Availability↑

CloneID	OHu39735
Clone ID Related Accession (Same CDS sequence)	NM_001330627.1 , NM_001330627.2
Accession Version	NM_001330627.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	calpastatin isoform q
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Aug 30, 2016 this sequence version replaced XM_006714705.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.75185.1, SRR1660807.111876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317556.1
CDS	267..2516
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330627.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330627.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( NM_001330627.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317556.1 Homo sapiens calpastatin (CAST), transcript variant 18, mRNA.

Price & Availability↑

CloneID	OHu39735
Clone ID Related Accession (Same CDS sequence)	NM_001330627.1 , NM_001330627.2
Accession Version	NM_001330627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	calpastatin isoform q
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020900.5, AC008906.5 and KC877037.1. On Jun 2, 2019 this sequence version replaced NM_001330627.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.201569.1, SRR1803616.75185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317556.1
CDS	48..2297
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	NM_001330627.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330627.2

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAA AGGAAGGGAT CACAGGGCCT 
CCTGCAGACT CTTCGAAACC CATAGGGCCA GATGATGCTA TAGACGCCTT GTCATCTGAC 
TTCACCTGTG GGTCGCCTAC AGCTGCTGGA AAGAAAACTG AAAAAGAGGA ATCTACAGAA 
GTTTTAAAAG CTCAGTCAGC AGGGACAGTC AGAAGTGCTG CTCCACCCCA AGAGAAGAAA 
AGAAAGGTGG AGAAGGATAC AATGAGTGAT CAAGCACTCG AGGCTCTGTC GGCTTCACTG 
GGCACCCGGC AAGCAGAACC TGAGCTCGAC CTCCGCTCAA TTAAGGAAGT CGATGAGGCA 
AAAGCTAAAG AAGAAAAACT AGAGAAGTGT GGTGAGGATG ATGAAACAAT CCCATCTGAG 
TACAGATTAA AACCAGCCAC GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714696.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714759.1 Homo sapiens calpastatin (CAST), transcript variant X1, mRNA.

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CloneID	OHu39726
Clone ID Related Accession (Same CDS sequence)	NM_001750.6 , XM_006714696.3 , NM_001750.7
Accession Version	XM_006714696.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714759.1
CDS	158..2533
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714696.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714696.3

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGCAGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714697.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714760.1 Homo sapiens calpastatin (CAST), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu39727
Clone ID Related Accession (Same CDS sequence)	XM_006714697.3
Accession Version	XM_006714697.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714760.1
CDS	175..2547
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714697.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714697.3

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GCAAAAGCTA AAGAAGAAAA ACTAGAGAAG TGTGGTGAGG ATGATGAAAC AATCCCATCT 
GAGTACAGAT TAAAACCAGC CACGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714698.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714761.1 Homo sapiens calpastatin (CAST), transcript variant X3, mRNA.

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CloneID	OHu39728
Clone ID Related Accession (Same CDS sequence)	XM_006714698.3
Accession Version	XM_006714698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT 
GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA 
ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT 
GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA 
CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC 
TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG 
GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA 
CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT 
GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA 
AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC 
TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT 
GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC 
CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT 
GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG 
ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGCAGAGTG ACAAAGACCT CGATGATGCC 
TTGGATAAAC TCTCTGACAG TCTAGGACAA AGGCAGCCTG ACCCAGATGA GAACAAACCA 
ATGGAAGATA AAGTAAAGGA AAAAGCTAAA GCTGAACATA GAGACAAGCT TGGAGAAAGA 
GATGACACTA TCCCACCTGA ATACAGACAT CTCCTGGATG ATAATGGACA GGACAAACCA 
GTGAAGCCAC CTACAAAGAA ATCAGAGGAT TCAAAGAAAC CTGCAGATGA CCAAGACCCC 
ATTGATGCTC TCTCAGGAGA TCTGGACAGC TGTCCCTCCA CTACAGAAAC CTCACAGAAC 
ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT TCCAGCTCCA AAGCACCTAA GAATGGAGGT 
AAAGCGAAGG ATTCAGCAAA GACAACAGAG GAAACTTCCA AGCCAAAAGA TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714761.1
CDS	175..2511
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714698.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714698.3

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT 
GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA 
ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT 
GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA 
CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC 
TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG 
GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA 
CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT 
GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA 
AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC 
TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT 
GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC 
CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT 
GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG 
ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGCAGAGTG ACAAAGACCT CGATGATGCC 
TTGGATAAAC TCTCTGACAG TCTAGGACAA AGGCAGCCTG ACCCAGATGA GAACAAACCA 
ATGGAAGATA AAGTAAAGGA AAAAGCTAAA GCTGAACATA GAGACAAGCT TGGAGAAAGA 
GATGACACTA TCCCACCTGA ATACAGACAT CTCCTGGATG ATAATGGACA GGACAAACCA 
GTGAAGCCAC CTACAAAGAA ATCAGAGGAT TCAAAGAAAC CTGCAGATGA CCAAGACCCC 
ATTGATGCTC TCTCAGGAGA TCTGGACAGC TGTCCCTCCA CTACAGAAAC CTCACAGAAC 
ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT TCCAGCTCCA AAGCACCTAA GAATGGAGGT 
AAAGCGAAGG ATTCAGCAAA GACAACAGAG GAAACTTCCA AGCCAAAAGA TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009913.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865402.1 Homo sapiens calpastatin (CAST), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu39730
Clone ID Related Accession (Same CDS sequence)	NM_001042441.3 , XM_017009913.1 , NM_001042441.2
Accession Version	XM_017009913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA 
GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA 
TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT 
ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG 
AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT 
TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT 
GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA 
TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT 
GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT 
GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG 
GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG 
TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC 
TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT 
AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA 
AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT 
GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT 
GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA 
CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC 
AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG 
GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT 
GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG 
AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA 
GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC 
AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA 
AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865402.1
CDS	173..2491
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009913.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009913.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA 
GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA 
TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT 
ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG 
AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT 
TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT 
GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA 
TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT 
GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT 
GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG 
GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG 
TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC 
TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT 
AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA 
AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT 
GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT 
GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA 
CCCCGTGATA CCTCGCAGAG TGACAAAGAC CTCGATGATG CCTTGGATAA ACTCTCTGAC 
AGTCTAGGAC AAAGGCAGCC TGACCCAGAT GAGAACAAAC CAATGGAAGA TAAAGTAAAG 
GAAAAAGCTA AAGCTGAACA TAGAGACAAG CTTGGAGAAA GAGATGACAC TATCCCACCT 
GAATACAGAC ATCTCCTGGA TGATAATGGA CAGGACAAAC CAGTGAAGCC ACCTACAAAG 
AAATCAGAGG ATTCAAAGAA ACCTGCAGAT GACCAAGACC CCATTGATGC TCTCTCAGGA 
GATCTGGACA GCTGTCCCTC CACTACAGAA ACCTCACAGA ACACAGCAAA GGATAAGTGC 
AAGAAGGCTG CTTCCAGCTC CAAAGCACCT AAGAATGGAG GTAAAGCGAA GGATTCAGCA 
AAGACAACAG AGGAAACTTC CAAGCCAAAA GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714701.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714764.1 Homo sapiens calpastatin (CAST), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu39731
Clone ID Related Accession (Same CDS sequence)	NM_001042442.3 , XM_006714701.3 , NM_001042442.2
Accession Version	XM_006714701.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714764.1
CDS	200..2509
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714701.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714701.3

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGCAGA GTGACAAAGA CCTCGATGAT GCCTTGGATA AACTCTCTGA CAGTCTAGGA 
CAAAGGCAGC CTGACCCAGA TGAGAACAAA CCAATGGAAG ATAAAGTAAA GGAAAAAGCT 
AAAGCTGAAC ATAGAGACAA GCTTGGAGAA AGAGATGACA CTATCCCACC TGAATACAGA 
CATCTCCTGG ATGATAATGG ACAGGACAAA CCAGTGAAGC CACCTACAAA GAAATCAGAG 
GATTCAAAGA AACCTGCAGA TGACCAAGAC CCCATTGATG CTCTCTCAGG AGATCTGGAC 
AGCTGTCCCT CCACTACAGA AACCTCACAG AACACAGCAA AGGATAAGTG CAAGAAGGCT 
GCTTCCAGCT CCAAAGCACC TAAGAATGGA GGTAAAGCGA AGGATTCAGC AAAGACAACA 
GAGGAAACTT CCAAGCCAAA AGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_006714703.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714766.1 Homo sapiens calpastatin (CAST), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu39733
Clone ID Related Accession (Same CDS sequence)	XM_006714703.3
Accession Version	XM_006714703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAACCCA TAGGGCCAGA TGATGCTATA 
GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA 
AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT 
CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG 
GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT 
AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT 
GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA 
TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT 
TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA 
GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG 
TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA 
CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG 
CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG 
GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT 
GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA 
GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714766.1
CDS	200..2470
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_006714703.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714703.3

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAACCCA TAGGGCCAGA TGATGCTATA 
GACGCCTTGT CATCTGACTT CACCTGTGGG TCGCCTACAG CTGCTGGAAA GAAAACTGAA 
AAAGAGGAAT CTACAGAAGT TTTAAAAGCT CAGTCAGCAG GGACAGTCAG AAGTGCTGCT 
CCACCCCAAG AGAAGAAAAG AAAGGTGGAG AAGGATACAA TGAGTGATCA AGCACTCGAG 
GCTCTGTCGG CTTCACTGGG CACCCGGCAA GCAGAACCTG AGCTCGACCT CCGCTCAATT 
AAGGAAGTCG ATGAGGCAAA AGCTAAAGAA GAAAAACTAG AGAAGTGTGG TGAGGATGAT 
GAAACAATCC CATCTGAGTA CAGATTAAAA CCAGCCACGG ATAAAGATGG AAAACCACTA 
TTGCCAGAGC CTGAAGAAAA ACCCAAGCCT CGGAGTGAAT CAGAACTCAT TGATGAACTT 
TCAGAAGATT TTGACCGGTC TGAATGTAAA GAGAAACCAT CTAAGCCAAC TGAAAAGACA 
GAAGAATCTA AGGCCGCTGC TCCAGCTCCT GTGTCGGAGG CTGTGTGTCG GACCTCCATG 
TGTAGTATAC AGTCAGCACC CCCTGAGCCG GCTACCTTGA AGGGCACAGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGA TAGCCTGGGG AAAAAGGAAG CAGATCCAGA AGATGGAAAA 
CCTGTGATGG ATAAAGTCAA GGAGAAGGCC AAAGAAGAAG ACCGTGAAAA GCTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAG 
CCACTCCTGC CAAAAGAGTC TAAGGAACAG CTTCCACCCA TGAGTGAAGA CTTCCTTCTG 
GATGCTTTGT CTGAGGACTT CTCTGGTCCA CAAAATGCTT CATCTCTTAA ATTTGAAGAT 
GCTAAACTTG CTGCTGCCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTTC AACGACCCAA 
GCTGGAGCCC CACCCCGTGA TACCTCGCAG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_011543654.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541956.1 Homo sapiens calpastatin (CAST), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu56642
Clone ID Related Accession (Same CDS sequence)	XM_011543654.2
Accession Version	XM_011543654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA AAAAAGAAAA GAAATCATTA ACCCCAGCTG TGCCAGTTGA ATCTAAACCG 
GATAAACCAT CGGGAAAGTC AGGCATGGAT GCTGCTTTGG ATGACTTAAT AGATACTTTA 
GGAGGACCTG AAGAAACTGA AGAAGAAAAT ACAACGTATA CTGGACCAGA AGTTTCAGAT 
CCAATGAGTT CCACCTACAT AGAGGAATTG GGTAAAAGAG AAGTCACAAT TCCTCCAAAA 
TATAGGGAAC TATTGGCTAA AAAGGAAGGG ATCACAGGGC CTCCTGCAGA CTCTTCGAAA 
CCCATAGGGC CAGATGATGC TATAGACGCC TTGTCATCTG ACTTCACCTG TGGGTCGCCT 
ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG GAATCTACAG AAGTTTTAAA AGCTCAGTCA 
GCAGGGACAG TCAGAAGTGC TGCTCCACCC CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT 
ACAATGAGTG ATCAAGCACT CGAGGCTCTG TCGGCTTCAC TGGGCACCCG GCAAGCAGAA 
CCTGAGCTCG ACCTCCGCTC AATTAAGGAA GTCGATGAGG CAAAAGCTAA AGAAGAAAAA 
CTAGAGAAGT GTGGTGAGGA TGATGAAACA ATCCCATCTG AGTACAGATT AAAACCAGCC 
ACGGATAAAG ATGGAAAACC ACTATTGCCA GAGCCTGAAG AAAAACCCAA GCCTCGGAGT 
GAATCAGAAC TCATTGATGA ACTTTCAGAA GATTTTGACC GGTCTGAATG TAAAGAGAAA 
CCATCTAAGC CAACTGAAAA GACAGAAGAA TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG 
GAGGCTGTGT GTCGGACCTC CATGTGTAGT ATACAGTCAG CACCCCCTGA GCCGGCTACC 
TTGAAGGGCA CAGTGCCAGA TGATGCTGTA GAAGCCTTGG CTGATAGCCT GGGGAAAAAG 
GAAGCAGATC CAGAAGATGG AAAACCTGTG ATGGATAAAG TCAAGGAGAA GGCCAAAGAA 
GAAGACCGTG AAAAGCTTGG TGAAAAAGAA GAAACAATTC CTCCTGATTA TAGATTAGAA 
GAGGTCAAGG ATAAAGATGG AAAGCCACTC CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA 
CCCATGAGTG AAGACTTCCT TCTGGATGCT TTGTCTGAGG ACTTCTCTGG TCCACAAAAT 
GCTTCATCTC TTAAATTTGA AGATGCTAAA CTTGCTGCTG CCATCTCTGA AGTGGTTTCC 
CAAACCCCAG CTTCAACGAC CCAAGCTGGA GCCCCACCCC GTGATACCTC GCAGAGTGAC 
AAAGACCTCG ATGATGCCTT GGATAAACTC TCTGACAGTC TAGGACAAAG GCAGCCTGAC 
CCAGATGAGA ACAAACCAAT GGAAGATAAA GTAAAGGAAA AAGCTAAAGC TGAACATAGA 
GACAAGCTTG GAGAAAGAGA TGACACTATC CCACCTGAAT ACAGACATCT CCTGGATGAT 
AATGGACAGG ACAAACCAGT GAAGCCACCT ACAAAGAAAT CAGAGGATTC AAAGAAACCT 
GCAGATGACC AAGACCCCAT TGATGCTCTC TCAGGAGATC TGGACAGCTG TCCCTCCACT 
ACAGAAACCT CACAGAACAC AGCAAAGGAT AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA 
GCACCTAAGA ATGGAGGTAA AGCGAAGGAT TCAGCAAAGA CAACAGAGGA AACTTCCAAG 
CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541956.1
CDS	175..2469
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_011543654.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543654.2

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA AAAAAGAAAA GAAATCATTA ACCCCAGCTG TGCCAGTTGA ATCTAAACCG 
GATAAACCAT CGGGAAAGTC AGGCATGGAT GCTGCTTTGG ATGACTTAAT AGATACTTTA 
GGAGGACCTG AAGAAACTGA AGAAGAAAAT ACAACGTATA CTGGACCAGA AGTTTCAGAT 
CCAATGAGTT CCACCTACAT AGAGGAATTG GGTAAAAGAG AAGTCACAAT TCCTCCAAAA 
TATAGGGAAC TATTGGCTAA AAAGGAAGGG ATCACAGGGC CTCCTGCAGA CTCTTCGAAA 
CCCATAGGGC CAGATGATGC TATAGACGCC TTGTCATCTG ACTTCACCTG TGGGTCGCCT 
ACAGCTGCTG GAAAGAAAAC TGAAAAAGAG GAATCTACAG AAGTTTTAAA AGCTCAGTCA 
GCAGGGACAG TCAGAAGTGC TGCTCCACCC CAAGAGAAGA AAAGAAAGGT GGAGAAGGAT 
ACAATGAGTG ATCAAGCACT CGAGGCTCTG TCGGCTTCAC TGGGCACCCG GCAAGCAGAA 
CCTGAGCTCG ACCTCCGCTC AATTAAGGAA GTCGATGAGG CAAAAGCTAA AGAAGAAAAA 
CTAGAGAAGT GTGGTGAGGA TGATGAAACA ATCCCATCTG AGTACAGATT AAAACCAGCC 
ACGGATAAAG ATGGAAAACC ACTATTGCCA GAGCCTGAAG AAAAACCCAA GCCTCGGAGT 
GAATCAGAAC TCATTGATGA ACTTTCAGAA GATTTTGACC GGTCTGAATG TAAAGAGAAA 
CCATCTAAGC CAACTGAAAA GACAGAAGAA TCTAAGGCCG CTGCTCCAGC TCCTGTGTCG 
GAGGCTGTGT GTCGGACCTC CATGTGTAGT ATACAGTCAG CACCCCCTGA GCCGGCTACC 
TTGAAGGGCA CAGTGCCAGA TGATGCTGTA GAAGCCTTGG CTGATAGCCT GGGGAAAAAG 
GAAGCAGATC CAGAAGATGG AAAACCTGTG ATGGATAAAG TCAAGGAGAA GGCCAAAGAA 
GAAGACCGTG AAAAGCTTGG TGAAAAAGAA GAAACAATTC CTCCTGATTA TAGATTAGAA 
GAGGTCAAGG ATAAAGATGG AAAGCCACTC CTGCCAAAAG AGTCTAAGGA ACAGCTTCCA 
CCCATGAGTG AAGACTTCCT TCTGGATGCT TTGTCTGAGG ACTTCTCTGG TCCACAAAAT 
GCTTCATCTC TTAAATTTGA AGATGCTAAA CTTGCTGCTG CCATCTCTGA AGTGGTTTCC 
CAAACCCCAG CTTCAACGAC CCAAGCTGGA GCCCCACCCC GTGATACCTC GCAGAGTGAC 
AAAGACCTCG ATGATGCCTT GGATAAACTC TCTGACAGTC TAGGACAAAG GCAGCCTGAC 
CCAGATGAGA ACAAACCAAT GGAAGATAAA GTAAAGGAAA AAGCTAAAGC TGAACATAGA 
GACAAGCTTG GAGAAAGAGA TGACACTATC CCACCTGAAT ACAGACATCT CCTGGATGAT 
AATGGACAGG ACAAACCAGT GAAGCCACCT ACAAAGAAAT CAGAGGATTC AAAGAAACCT 
GCAGATGACC AAGACCCCAT TGATGCTCTC TCAGGAGATC TGGACAGCTG TCCCTCCACT 
ACAGAAACCT CACAGAACAC AGCAAAGGAT AAGTGCAAGA AGGCTGCTTC CAGCTCCAAA 
GCACCTAAGA ATGGAGGTAA AGCGAAGGAT TCAGCAAAGA CAACAGAGGA AACTTCCAAG 
CCAAAAGATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_011543655.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541957.1 Homo sapiens calpastatin (CAST), transcript variant X15, mRNA.

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CloneID	OHu56643
Clone ID Related Accession (Same CDS sequence)	XM_011543655.2
Accession Version	XM_011543655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA 
TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA 
TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG 
GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG 
AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA 
GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA 
GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC 
ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT 
TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA 
GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA 
GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC 
AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT 
GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA 
GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA 
GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA 
CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA 
AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541957.1
CDS	175..2466
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_011543655.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543655.2

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AAAAGGAAGG GATCACAGGG 
CCTCCTGCAG ACTCTTCGAA ACCCATAGGG CCAGATGATG CTATAGACGC CTTGTCATCT 
GACTTCACCT GTGGGTCGCC TACAGCTGCT GGAAAGAAAA CTGAAAAAGA GGAATCTACA 
GAAGTTTTAA AAGCTCAGTC AGCAGGGACA GTCAGAAGTG CTGCTCCACC CCAAGAGAAG 
AAAAGAAAGG TGGAGAAGGA TACAATGAGT GATCAAGCAC TCGAGGCTCT GTCGGCTTCA 
CTGGGCACCC GGCAAGCAGA ACCTGAGCTC GACCTCCGCT CAATTAAGGA AGTCGATGAG 
GATAAAGATG GAAAACCACT ATTGCCAGAG CCTGAAGAAA AACCCAAGCC TCGGAGTGAA 
TCAGAACTCA TTGATGAACT TTCAGAAGAT TTTGACCGGT CTGAATGTAA AGAGAAACCA 
TCTAAGCCAA CTGAAAAGAC AGAAGAATCT AAGGCCGCTG CTCCAGCTCC TGTGTCGGAG 
GCTGTGTGTC GGACCTCCAT GTGTAGTATA CAGTCAGCAC CCCCTGAGCC GGCTACCTTG 
AAGGGCACAG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG ATAGCCTGGG GAAAAAGGAA 
GCAGATCCAG AAGATGGAAA ACCTGTGATG GATAAAGTCA AGGAGAAGGC CAAAGAAGAA 
GACCGTGAAA AGCTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA GCCACTCCTG CCAAAAGAGT CTAAGGAACA GCTTCCACCC 
ATGAGTGAAG ACTTCCTTCT GGATGCTTTG TCTGAGGACT TCTCTGGTCC ACAAAATGCT 
TCATCTCTTA AATTTGAAGA TGCTAAACTT GCTGCTGCCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTT CAACGACCCA AGCTGGAGCC CCACCCCGTG ATACCTCGCA GAGTGACAAA 
GACCTCGATG ATGCCTTGGA TAAACTCTCT GACAGTCTAG GACAAAGGCA GCCTGACCCA 
GATGAGAACA AACCAATGGA AGATAAAGTA AAGGAAAAAG CTAAAGCTGA ACATAGAGAC 
AAGCTTGGAG AAAGAGATGA CACTATCCCA CCTGAATACA GACATCTCCT GGATGATAAT 
GGACAGGACA AACCAGTGAA GCCACCTACA AAGAAATCAG AGGATTCAAA GAAACCTGCA 
GATGACCAAG ACCCCATTGA TGCTCTCTCA GGAGATCTGG ACAGCTGTCC CTCCACTACA 
GAAACCTCAC AGAACACAGC AAAGGATAAG TGCAAGAAGG CTGCTTCCAG CTCCAAAGCA 
CCTAAGAATG GAGGTAAAGC GAAGGATTCA GCAAAGACAA CAGAGGAAAC TTCCAAGCCA 
AAAGATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_011543656.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541958.1 Homo sapiens calpastatin (CAST), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu56644
Clone ID Related Accession (Same CDS sequence)	XM_011543656.2
Accession Version	XM_011543656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGAAAAAAG AAAAGAAATC ATTAACCCCA GCTGTGCCAG TTGAATCTAA ACCGGATAAA 
CCATCGGGAA AGTCAGGCAT GGATGCTGCT TTGGATGACT TAATAGATAC TTTAGGAGGA 
CCTGAAGAAA CTGAAGAAGA AAATACAACG TATACTGGAC CAGAAGTTTC AGATCCAATG 
AGTTCCACCT ACATAGAGGA ATTGGGTAAA AGAGAAGTCA CAATTCCTCC AAAATATAGG 
GAACTATTGG CTAAAAAGGA AGGGATCACA GGGCCTCCTG CAGACTCTTC GAAACCCATA 
GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT 
GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG 
ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG 
AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG 
CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG 
AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT 
AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA 
GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT 
AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT 
GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG 
GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA 
GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC 
CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC 
AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG 
AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA 
TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC 
CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGCAGAG TGACAAAGAC 
CTCGATGATG CCTTGGATAA ACTCTCTGAC AGTCTAGGAC AAAGGCAGCC TGACCCAGAT 
GAGAACAAAC CAATGGAAGA TAAAGTAAAG GAAAAAGCTA AAGCTGAACA TAGAGACAAG 
CTTGGAGAAA GAGATGACAC TATCCCACCT GAATACAGAC ATCTCCTGGA TGATAATGGA 
CAGGACAAAC CAGTGAAGCC ACCTACAAAG AAATCAGAGG ATTCAAAGAA ACCTGCAGAT 
GACCAAGACC CCATTGATGC TCTCTCAGGA GATCTGGACA GCTGTCCCTC CACTACAGAA 
ACCTCACAGA ACACAGCAAA GGATAAGTGC AAGAAGGCTG CTTCCAGCTC CAAAGCACCT 
AAGAATGGAG GTAAAGCGAA GGATTCAGCA AAGACAACAG AGGAAACTTC CAAGCCAAAA 
GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541958.1
CDS	185..2413
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_011543656.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543656.2

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGAAAAAAG AAAAGAAATC ATTAACCCCA GCTGTGCCAG TTGAATCTAA ACCGGATAAA 
CCATCGGGAA AGTCAGGCAT GGATGCTGCT TTGGATGACT TAATAGATAC TTTAGGAGGA 
CCTGAAGAAA CTGAAGAAGA AAATACAACG TATACTGGAC CAGAAGTTTC AGATCCAATG 
AGTTCCACCT ACATAGAGGA ATTGGGTAAA AGAGAAGTCA CAATTCCTCC AAAATATAGG 
GAACTATTGG CTAAAAAGGA AGGGATCACA GGGCCTCCTG CAGACTCTTC GAAACCCATA 
GGGCCAGATG ATGCTATAGA CGCCTTGTCA TCTGACTTCA CCTGTGGGTC GCCTACAGCT 
GCTGGAAAGA AAACTGAAAA AGAGGAATCT ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG 
ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG AAGAAAAGAA AGGTGGAGAA GGATACAATG 
AGTGATCAAG CACTCGAGGC TCTGTCGGCT TCACTGGGCA CCCGGCAAGC AGAACCTGAG 
CTCGACCTCC GCTCAATTAA GGAAGTCGAT GAGGCAAAAG CTAAAGAAGA AAAACTAGAG 
AAGTGTGGTG AGGATGATGA AACAATCCCA TCTGAGTACA GATTAAAACC AGCCACGGAT 
AAAGATGGAA AACCACTATT GCCAGAGCCT GAAGAAAAAC CCAAGCCTCG GAGTGAATCA 
GAACTCATTG ATGAACTTTC AGAAGATTTT GACCGGTCTG AATGTAAAGA GAAACCATCT 
AAGCCAACTG AAAAGACAGA AGAATCTAAG GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT 
GTGTGTCGGA CCTCCATGTG TAGTATACAG TCAGCACCCC CTGAGCCGGC TACCTTGAAG 
GGCACAGTGC CAGATGATGC TGTAGAAGCC TTGGCTGATA GCCTGGGGAA AAAGGAAGCA 
GATCCAGAAG ATGGAAAACC TGTGATGGAT AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC 
CGTGAAAAGC TTGGTGAAAA AGAAGAAACA ATTCCTCCTG ATTATAGATT AGAAGAGGTC 
AAGGATAAAG ATGGAAAGCC ACTCCTGCCA AAAGAGTCTA AGGAACAGCT TCCACCCATG 
AGTGAAGACT TCCTTCTGGA TGCTTTGTCT GAGGACTTCT CTGGTCCACA AAATGCTTCA 
TCTCTTAAAT TTGAAGATGC TAAACTTGCT GCTGCCATCT CTGAAGTGGT TTCCCAAACC 
CCAGCTTCAA CGACCCAAGC TGGAGCCCCA CCCCGTGATA CCTCGCAGAG TGACAAAGAC 
CTCGATGATG CCTTGGATAA ACTCTCTGAC AGTCTAGGAC AAAGGCAGCC TGACCCAGAT 
GAGAACAAAC CAATGGAAGA TAAAGTAAAG GAAAAAGCTA AAGCTGAACA TAGAGACAAG 
CTTGGAGAAA GAGATGACAC TATCCCACCT GAATACAGAC ATCTCCTGGA TGATAATGGA 
CAGGACAAAC CAGTGAAGCC ACCTACAAAG AAATCAGAGG ATTCAAAGAA ACCTGCAGAT 
GACCAAGACC CCATTGATGC TCTCTCAGGA GATCTGGACA GCTGTCCCTC CACTACAGAA 
ACCTCACAGA ACACAGCAAA GGATAAGTGC AAGAAGGCTG CTTCCAGCTC CAAAGCACCT 
AAGAATGGAG GTAAAGCGAA GGATTCAGCA AAGACAACAG AGGAAACTTC CAAGCCAAAA 
GATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009911.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865400.1 Homo sapiens calpastatin (CAST), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu96112
Clone ID Related Accession (Same CDS sequence)	XM_017009911.1 , XM_017009912.1
Accession Version	XM_017009911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT 
GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA 
ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT 
GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA 
CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC 
TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG 
GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA 
CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT 
GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA 
AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC 
TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT 
GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC 
CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT 
GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG 
ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGAGTGACA AAGACCTCGA TGATGCCTTG 
GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG 
GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT 
GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG 
AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT 
GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA 
GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA 
GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865400.1
CDS	175..2508
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009911.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009911.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT 
GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA 
ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT 
GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA 
CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC 
TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG 
GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA 
CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT 
GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA 
AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC 
TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT 
GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC 
CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT 
GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG 
ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGAGTGACA AAGACCTCGA TGATGCCTTG 
GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG 
GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT 
GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG 
AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT 
GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA 
GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA 
GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009912.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865401.1 Homo sapiens calpastatin (CAST), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu96112
Clone ID Related Accession (Same CDS sequence)	XM_017009911.1 , XM_017009912.1
Accession Version	XM_017009912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT 
GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA 
ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT 
GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA 
CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC 
TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG 
GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA 
CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT 
GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA 
AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC 
TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT 
GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC 
CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT 
GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG 
ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGAGTGACA AAGACCTCGA TGATGCCTTG 
GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG 
GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT 
GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG 
AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT 
GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA 
GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA 
GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865401.1
CDS	186..2519
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009912.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009912.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT 
GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA 
ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT 
GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA 
CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC 
TCAATTAAGG AAGTCGATGA GGCAAAAGCT AAAGAAGAAA AACTAGAGAA GTGTGGTGAG 
GATGATGAAA CAATCCCATC TGAGTACAGA TTAAAACCAG CCACGGATAA AGATGGAAAA 
CCACTATTGC CAGAGCCTGA AGAAAAACCC AAGCCTCGGA GTGAATCAGA ACTCATTGAT 
GAACTTTCAG AAGATTTTGA CCGGTCTGAA TGTAAAGAGA AACCATCTAA GCCAACTGAA 
AAGACAGAAG AATCTAAGGC CGCTGCTCCA GCTCCTGTGT CGGAGGCTGT GTGTCGGACC 
TCCATGTGTA GTATACAGTC AGCACCCCCT GAGCCGGCTA CCTTGAAGGG CACAGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGATAGC CTGGGGAAAA AGGAAGCAGA TCCAGAAGAT 
GGAAAACCTG TGATGGATAA AGTCAAGGAG AAGGCCAAAG AAGAAGACCG TGAAAAGCTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAGCCAC TCCTGCCAAA AGAGTCTAAG GAACAGCTTC CACCCATGAG TGAAGACTTC 
CTTCTGGATG CTTTGTCTGA GGACTTCTCT GGTCCACAAA ATGCTTCATC TCTTAAATTT 
GAAGATGCTA AACTTGCTGC TGCCATCTCT GAAGTGGTTT CCCAAACCCC AGCTTCAACG 
ACCCAAGCTG GAGCCCCACC CCGTGATACC TCGAGTGACA AAGACCTCGA TGATGCCTTG 
GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG 
GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT 
GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG 
AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT 
GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA 
GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA 
GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009914.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865403.1 Homo sapiens calpastatin (CAST), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu96113
Clone ID Related Accession (Same CDS sequence)	XM_017009914.1
Accession Version	XM_017009914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA 
GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA 
TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT 
ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG 
AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT 
TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT 
GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA 
TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT 
GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT 
GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG 
GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG 
TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC 
TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT 
AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA 
AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT 
GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT 
GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA 
CCCCGTGATA CCTCGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865403.1
CDS	175..2490
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009914.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009914.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAAAAGGA AGGGATCACA 
GGGCCTCCTG CAGACTCTTC GAAACCCATA GGGCCAGATG ATGCTATAGA CGCCTTGTCA 
TCTGACTTCA CCTGTGGGTC GCCTACAGCT GCTGGAAAGA AAACTGAAAA AGAGGAATCT 
ACAGAAGTTT TAAAAGCTCA GTCAGCAGGG ACAGTCAGAA GTGCTGCTCC ACCCCAAGAG 
AAGAAAAGAA AGGTGGAGAA GGATACAATG AGTGATCAAG CACTCGAGGC TCTGTCGGCT 
TCACTGGGCA CCCGGCAAGC AGAACCTGAG CTCGACCTCC GCTCAATTAA GGAAGTCGAT 
GAGGCAAAAG CTAAAGAAGA AAAACTAGAG AAGTGTGGTG AGGATGATGA AACAATCCCA 
TCTGAGTACA GATTAAAACC AGCCACGGAT AAAGATGGAA AACCACTATT GCCAGAGCCT 
GAAGAAAAAC CCAAGCCTCG GAGTGAATCA GAACTCATTG ATGAACTTTC AGAAGATTTT 
GACCGGTCTG AATGTAAAGA GAAACCATCT AAGCCAACTG AAAAGACAGA AGAATCTAAG 
GCCGCTGCTC CAGCTCCTGT GTCGGAGGCT GTGTGTCGGA CCTCCATGTG TAGTATACAG 
TCAGCACCCC CTGAGCCGGC TACCTTGAAG GGCACAGTGC CAGATGATGC TGTAGAAGCC 
TTGGCTGATA GCCTGGGGAA AAAGGAAGCA GATCCAGAAG ATGGAAAACC TGTGATGGAT 
AAAGTCAAGG AGAAGGCCAA AGAAGAAGAC CGTGAAAAGC TTGGTGAAAA AGAAGAAACA 
ATTCCTCCTG ATTATAGATT AGAAGAGGTC AAGGATAAAG ATGGAAAGCC ACTCCTGCCA 
AAAGAGTCTA AGGAACAGCT TCCACCCATG AGTGAAGACT TCCTTCTGGA TGCTTTGTCT 
GAGGACTTCT CTGGTCCACA AAATGCTTCA TCTCTTAAAT TTGAAGATGC TAAACTTGCT 
GCTGCCATCT CTGAAGTGGT TTCCCAAACC CCAGCTTCAA CGACCCAAGC TGGAGCCCCA 
CCCCGTGATA CCTCGAGTGA CAAAGACCTC GATGATGCCT TGGATAAACT CTCTGACAGT 
CTAGGACAAA GGCAGCCTGA CCCAGATGAG AACAAACCAA TGGAAGATAA AGTAAAGGAA 
AAAGCTAAAG CTGAACATAG AGACAAGCTT GGAGAAAGAG ATGACACTAT CCCACCTGAA 
TACAGACATC TCCTGGATGA TAATGGACAG GACAAACCAG TGAAGCCACC TACAAAGAAA 
TCAGAGGATT CAAAGAAACC TGCAGATGAC CAAGACCCCA TTGATGCTCT CTCAGGAGAT 
CTGGACAGCT GTCCCTCCAC TACAGAAACC TCACAGAACA CAGCAAAGGA TAAGTGCAAG 
AAGGCTGCTT CCAGCTCCAA AGCACCTAAG AATGGAGGTA AAGCGAAGGA TTCAGCAAAG 
ACAACAGAGG AAACTTCCAA GCCAAAAGAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009916.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865405.1 Homo sapiens calpastatin (CAST), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu96114
Clone ID Related Accession (Same CDS sequence)	XM_017009916.1
Accession Version	XM_017009916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGAGTG ACAAAGACCT CGATGATGCC TTGGATAAAC TCTCTGACAG TCTAGGACAA 
AGGCAGCCTG ACCCAGATGA GAACAAACCA ATGGAAGATA AAGTAAAGGA AAAAGCTAAA 
GCTGAACATA GAGACAAGCT TGGAGAAAGA GATGACACTA TCCCACCTGA ATACAGACAT 
CTCCTGGATG ATAATGGACA GGACAAACCA GTGAAGCCAC CTACAAAGAA ATCAGAGGAT 
TCAAAGAAAC CTGCAGATGA CCAAGACCCC ATTGATGCTC TCTCAGGAGA TCTGGACAGC 
TGTCCCTCCA CTACAGAAAC CTCACAGAAC ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT 
TCCAGCTCCA AAGCACCTAA GAATGGAGGT AAAGCGAAGG ATTCAGCAAA GACAACAGAG 
GAAACTTCCA AGCCAAAAGA TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865405.1
CDS	200..2506
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009916.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009916.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCTGTCTGT GGTTCATGAG AAAAAATCCC AAGAAGGAAA GCCAAAAGAA 
CACACAGAGC CAAAAAGCCT ACCCAAGCAG GCATCAGATA CAGGAAGTAA CGATGCTCAC 
AATAAAAAAG CAGTTTCCAG ATCAGCTGAA CAGCAGCCAT CAGAGAAATC AACAGAACCA 
AAGACTAAAC CACAAGACAT GATTTCTGCT GGTGGAGAGA GTGTTGCTGG TATCACTGCA 
ATATCTGGCA AGCCGGGTGA CAAGAAAAAA GAAAAGAAAT CATTAACCCC AGCTGTGCCA 
GTTGAATCTA AACCGGATAA ACCATCGGGA AAGTCAGGCA TGGATGCTGC TTTGGATGAC 
TTAATAGATA CTTTAGGAGG ACCTGAAGAA ACTGAAGAAG AAAATACAAC GTATACTGGA 
CCAGAAGTTT CAGATCCAAT GAGTTCCACC TACATAGAGG AATTGGGTAA AAGAGAAGTC 
ACAATTCCTC CAAAATATAG GGAACTATTG GCTAAAAAGG AAGGGATCAC AGGGCCTCCT 
GCAGACTCTT CGAAACCCAT AGGGCCAGAT GATGCTATAG ACGCCTTGTC ATCTGACTTC 
ACCTGTGGGT CGCCTACAGC TGCTGGAAAG AAAACTGAAA AAGAGGAATC TACAGAAGTT 
TTAAAAGCTC AGTCAGCAGG GACAGTCAGA AGTGCTGCTC CACCCCAAGA GAAGAAAAGA 
AAGGTGGAGA AGGATACAAT GAGTGATCAA GCACTCGAGG CTCTGTCGGC TTCACTGGGC 
ACCCGGCAAG CAGAACCTGA GCTCGACCTC CGCTCAATTA AGGAAGTCGA TGAGGCAAAA 
GCTAAAGAAG AAAAACTAGA GAAGTGTGGT GAGGATGATG AAACAATCCC ATCTGAGTAC 
AGATTAAAAC CAGCCACGGA TAAAGATGGA AAACCACTAT TGCCAGAGCC TGAAGAAAAA 
CCCAAGCCTC GGAGTGAATC AGAACTCATT GATGAACTTT CAGAAGATTT TGACCGGTCT 
GAATGTAAAG AGAAACCATC TAAGCCAACT GAAAAGACAG AAGAATCTAA GGCCGCTGCT 
CCAGCTCCTG TGTCGGAGGC TGTGTGTCGG ACCTCCATGT GTAGTATACA GTCAGCACCC 
CCTGAGCCGG CTACCTTGAA GGGCACAGTG CCAGATGATG CTGTAGAAGC CTTGGCTGAT 
AGCCTGGGGA AAAAGGAAGC AGATCCAGAA GATGGAAAAC CTGTGATGGA TAAAGTCAAG 
GAGAAGGCCA AAGAAGAAGA CCGTGAAAAG CTTGGTGAAA AAGAAGAAAC AATTCCTCCT 
GATTATAGAT TAGAAGAGGT CAAGGATAAA GATGGAAAGC CACTCCTGCC AAAAGAGTCT 
AAGGAACAGC TTCCACCCAT GAGTGAAGAC TTCCTTCTGG ATGCTTTGTC TGAGGACTTC 
TCTGGTCCAC AAAATGCTTC ATCTCTTAAA TTTGAAGATG CTAAACTTGC TGCTGCCATC 
TCTGAAGTGG TTTCCCAAAC CCCAGCTTCA ACGACCCAAG CTGGAGCCCC ACCCCGTGAT 
ACCTCGAGTG ACAAAGACCT CGATGATGCC TTGGATAAAC TCTCTGACAG TCTAGGACAA 
AGGCAGCCTG ACCCAGATGA GAACAAACCA ATGGAAGATA AAGTAAAGGA AAAAGCTAAA 
GCTGAACATA GAGACAAGCT TGGAGAAAGA GATGACACTA TCCCACCTGA ATACAGACAT 
CTCCTGGATG ATAATGGACA GGACAAACCA GTGAAGCCAC CTACAAAGAA ATCAGAGGAT 
TCAAAGAAAC CTGCAGATGA CCAAGACCCC ATTGATGCTC TCTCAGGAGA TCTGGACAGC 
TGTCCCTCCA CTACAGAAAC CTCACAGAAC ACAGCAAAGG ATAAGTGCAA GAAGGCTGCT 
TCCAGCTCCA AAGCACCTAA GAATGGAGGT AAAGCGAAGG ATTCAGCAAA GACAACAGAG 
GAAACTTCCA AGCCAAAAGA TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009919.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865408.1 Homo sapiens calpastatin (CAST), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu96115
Clone ID Related Accession (Same CDS sequence)	XM_017009919.1
Accession Version	XM_017009919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT 
GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA 
ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT 
GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA 
CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC 
TCAATTAAGG AAGTCGATGA GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865408.1
CDS	175..2427
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009919.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009919.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCT GTCTGTGGTT CATGAGAAAA AATCCCAAGA AGGAAAGCCA 
AAAGAACACA CAGAGCCAAA AAGCCTACCC AAGCAGGCAT CAGATACAGG AAGTAACGAT 
GCTCACAATA AAAAAGCAGT TTCCAGATCA GCTGAACAGC AGCCATCAGA GAAATCAACA 
GAACCAAAGA CTAAACCACA AGACATGATT TCTGCTGGTG GAGAGAGTGT TGCTGGTATC 
ACTGCAATAT CTGGCAAGCC GGGTGACAAG AAAAAAGAAA AGAAATCATT AACCCCAGCT 
GTGCCAGTTG AATCTAAACC GGATAAACCA TCGGGAAAGT CAGGCATGGA TGCTGCTTTG 
GATGACTTAA TAGATACTTT AGGAGGACCT GAAGAAACTG AAGAAGAAAA TACAACGTAT 
ACTGGACCAG AAGTTTCAGA TCCAATGAGT TCCACCTACA TAGAGGAATT GGGTAAAAGA 
GAAGTCACAA TTCCTCCAAA ATATAGGGAA CTATTGGCTA AACCCATAGG GCCAGATGAT 
GCTATAGACG CCTTGTCATC TGACTTCACC TGTGGGTCGC CTACAGCTGC TGGAAAGAAA 
ACTGAAAAAG AGGAATCTAC AGAAGTTTTA AAAGCTCAGT CAGCAGGGAC AGTCAGAAGT 
GCTGCTCCAC CCCAAGAGAA GAAAAGAAAG GTGGAGAAGG ATACAATGAG TGATCAAGCA 
CTCGAGGCTC TGTCGGCTTC ACTGGGCACC CGGCAAGCAG AACCTGAGCT CGACCTCCGC 
TCAATTAAGG AAGTCGATGA GGATAAAGAT GGAAAACCAC TATTGCCAGA GCCTGAAGAA 
AAACCCAAGC CTCGGAGTGA ATCAGAACTC ATTGATGAAC TTTCAGAAGA TTTTGACCGG 
TCTGAATGTA AAGAGAAACC ATCTAAGCCA ACTGAAAAGA CAGAAGAATC TAAGGCCGCT 
GCTCCAGCTC CTGTGTCGGA GGCTGTGTGT CGGACCTCCA TGTGTAGTAT ACAGTCAGCA 
CCCCCTGAGC CGGCTACCTT GAAGGGCACA GTGCCAGATG ATGCTGTAGA AGCCTTGGCT 
GATAGCCTGG GGAAAAAGGA AGCAGATCCA GAAGATGGAA AACCTGTGAT GGATAAAGTC 
AAGGAGAAGG CCAAAGAAGA AGACCGTGAA AAGCTTGGTG AAAAAGAAGA AACAATTCCT 
CCTGATTATA GATTAGAAGA GGTCAAGGAT AAAGATGGAA AGCCACTCCT GCCAAAAGAG 
TCTAAGGAAC AGCTTCCACC CATGAGTGAA GACTTCCTTC TGGATGCTTT GTCTGAGGAC 
TTCTCTGGTC CACAAAATGC TTCATCTCTT AAATTTGAAG ATGCTAAACT TGCTGCTGCC 
ATCTCTGAAG TGGTTTCCCA AACCCCAGCT TCAACGACCC AAGCTGGAGC CCCACCCCGT 
GATACCTCGC AGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009922.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865411.1 Homo sapiens calpastatin (CAST), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OHu96118
Clone ID Related Accession (Same CDS sequence)	XM_017009922.1
Accession Version	XM_017009922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAC CCATAGGGCC AGATGATGCT 
ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT 
GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT 
GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC 
GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA 
ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT 
GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA 
CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA 
CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG 
ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC 
ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA 
AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT 
CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA 
GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC 
CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA TGATGCCTTG 
GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG 
GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT 
GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG 
AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT 
GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA 
GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA 
GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865411.1
CDS	175..2388
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009922.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009922.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAC CCATAGGGCC AGATGATGCT 
ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT 
GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT 
GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC 
GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA 
ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT 
GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA 
CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA 
CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG 
ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC 
ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA 
AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT 
CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA 
GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC 
CAAGCTGGAG CCCCACCCCG TGATACCTCG CAGAGTGACA AAGACCTCGA TGATGCCTTG 
GATAAACTCT CTGACAGTCT AGGACAAAGG CAGCCTGACC CAGATGAGAA CAAACCAATG 
GAAGATAAAG TAAAGGAAAA AGCTAAAGCT GAACATAGAG ACAAGCTTGG AGAAAGAGAT 
GACACTATCC CACCTGAATA CAGACATCTC CTGGATGATA ATGGACAGGA CAAACCAGTG 
AAGCCACCTA CAAAGAAATC AGAGGATTCA AAGAAACCTG CAGATGACCA AGACCCCATT 
GATGCTCTCT CAGGAGATCT GGACAGCTGT CCCTCCACTA CAGAAACCTC ACAGAACACA 
GCAAAGGATA AGTGCAAGAA GGCTGCTTCC AGCTCCAAAG CACCTAAGAA TGGAGGTAAA 
GCGAAGGATT CAGCAAAGAC AACAGAGGAA ACTTCCAAGC CAAAAGATGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009923.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865412.1 Homo sapiens calpastatin (CAST), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu96119
Clone ID Related Accession (Same CDS sequence)	XM_017009923.1
Accession Version	XM_017009923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAC CCATAGGGCC AGATGATGCT 
ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT 
GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT 
GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC 
GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA 
ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT 
GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA 
CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA 
CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG 
ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC 
ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA 
AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT 
CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA 
GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC 
CAAGCTGGAG CCCCACCCCG TGATACCTCG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865412.1
CDS	175..2385
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009923.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009923.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGTAAAAA CAGAACCTGA GAAGAAGTCA 
CAGTCAACCA AGCCAAAAAG CCTACCCAAG CAGGCATCAG ATACAGGAAG TAACGATGCT 
CACAATAAAA AAGCAGTTTC CAGATCAGCT GAACAGCAGC CATCAGAGAA ATCAACAGAA 
CCAAAGACTA AACCACAAGA CATGATTTCT GCTGGTGGAG AGAGTGTTGC TGGTATCACT 
GCAATATCTG GCAAGCCGGG TGACAAGAAA AAAGAAAAGA AATCATTAAC CCCAGCTGTG 
CCAGTTGAAT CTAAACCGGA TAAACCATCG GGAAAGTCAG GCATGGATGC TGCTTTGGAT 
GACTTAATAG ATACTTTAGG AGGACCTGAA GAAACTGAAG AAGAAAATAC AACGTATACT 
GGACCAGAAG TTTCAGATCC AATGAGTTCC ACCTACATAG AGGAATTGGG TAAAAGAGAA 
GTCACAATTC CTCCAAAATA TAGGGAACTA TTGGCTAAAC CCATAGGGCC AGATGATGCT 
ATAGACGCCT TGTCATCTGA CTTCACCTGT GGGTCGCCTA CAGCTGCTGG AAAGAAAACT 
GAAAAAGAGG AATCTACAGA AGTTTTAAAA GCTCAGTCAG CAGGGACAGT CAGAAGTGCT 
GCTCCACCCC AAGAGAAGAA AAGAAAGGTG GAGAAGGATA CAATGAGTGA TCAAGCACTC 
GAGGCTCTGT CGGCTTCACT GGGCACCCGG CAAGCAGAAC CTGAGCTCGA CCTCCGCTCA 
ATTAAGGAAG TCGATGAGGC AAAAGCTAAA GAAGAAAAAC TAGAGAAGTG TGGTGAGGAT 
GATGAAACAA TCCCATCTGA GTACAGATTA AAACCAGCCA CGGATAAAGA TGGAAAACCA 
CTATTGCCAG AGCCTGAAGA AAAACCCAAG CCTCGGAGTG AATCAGAACT CATTGATGAA 
CTTTCAGAAG ATTTTGACCG GTCTGAATGT AAAGAGAAAC CATCTAAGCC AACTGAAAAG 
ACAGAAGAAT CTAAGGCCGC TGCTCCAGCT CCTGTGTCGG AGGCTGTGTG TCGGACCTCC 
ATGTGTAGTA TACAGTCAGC ACCCCCTGAG CCGGCTACCT TGAAGGGCAC AGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGATAGCCTG GGGAAAAAGG AAGCAGATCC AGAAGATGGA 
AAACCTGTGA TGGATAAAGT CAAGGAGAAG GCCAAAGAAG AAGACCGTGA AAAGCTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAGCCACTCC TGCCAAAAGA GTCTAAGGAA CAGCTTCCAC CCATGAGTGA AGACTTCCTT 
CTGGATGCTT TGTCTGAGGA CTTCTCTGGT CCACAAAATG CTTCATCTCT TAAATTTGAA 
GATGCTAAAC TTGCTGCTGC CATCTCTGAA GTGGTTTCCC AAACCCCAGC TTCAACGACC 
CAAGCTGGAG CCCCACCCCG TGATACCTCG AGTGACAAAG ACCTCGATGA TGCCTTGGAT 
AAACTCTCTG ACAGTCTAGG ACAAAGGCAG CCTGACCCAG ATGAGAACAA ACCAATGGAA 
GATAAAGTAA AGGAAAAAGC TAAAGCTGAA CATAGAGACA AGCTTGGAGA AAGAGATGAC 
ACTATCCCAC CTGAATACAG ACATCTCCTG GATGATAATG GACAGGACAA ACCAGTGAAG 
CCACCTACAA AGAAATCAGA GGATTCAAAG AAACCTGCAG ATGACCAAGA CCCCATTGAT 
GCTCTCTCAG GAGATCTGGA CAGCTGTCCC TCCACTACAG AAACCTCACA GAACACAGCA 
AAGGATAAGT GCAAGAAGGC TGCTTCCAGC TCCAAAGCAC CTAAGAATGG AGGTAAAGCG 
AAGGATTCAG CAAAGACAAC AGAGGAAACT TCCAAGCCAA AAGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_017009924.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865413.1 Homo sapiens calpastatin (CAST), transcript variant X28, mRNA.

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CloneID	OHu96120
Clone ID Related Accession (Same CDS sequence)	XM_017009924.1
Accession Version	XM_017009924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	calpastatin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT 
GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG 
AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA 
AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA 
GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC 
CGCTCAATTA AGGAAGTCGA TGAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865413.1
CDS	175..2367
Translation	MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Target ORF information:

RefSeq Version	XM_017009924.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calpastatin (CAST), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009924.1

ORF Insert Sequence:

ATGTCCCAGC CCGGCCAGAA GCCCGCCGCC TCCCCGCGGC CCCGGCGAGC AGCCGCCGCC 
CGCCGCACCC ATGAGCATGT CAGTGAAAAA ACCAGTGAAT CGCCTTCCAA ACCAGGAGAA 
AAGAAAGGAT CAGATGAGAA AAAAGCAGCA AGCCTCGGCA GCAGTCAATC CTCCAGAACC 
TATGCTGGTG GAACAGCCTC GGCCACCAAG GTGTCAGCTT CCTCTGGTGC AACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCCATTCCAG TCAGCCAACA GATGGAAGGA 
CCACATCTTC CTAACAAGAA AAAACACAAA AAACAGGCTG TAAAAACAGA ACCTGAGAAG 
AAGTCACAGT CAACCAAGCC AAAAAGCCTA CCCAAGCAGG CATCAGATAC AGGAAGTAAC 
GATGCTCACA ATAAAAAAGC AGTTTCCAGA TCAGCTGAAC AGCAGCCATC AGAGAAATCA 
ACAGAACCAA AGACTAAACC ACAAGACATG ATTTCTGCTG GTGGAGAGAG TGTTGCTGGT 
ATCACTGCAA TATCTGGCAA GCCGGGTGAC AAGAAAAAAG AAAAGAAATC ATTAACCCCA 
GCTGTGCCAG TTGAATCTAA ACCGGATAAA CCATCGGGAA AGTCAGGCAT GGATGCTGCT 
TTGGATGACT TAATAGATAC TTTAGGAGGA CCTGAAGAAA CTGAAGAAGA AAATACAACG 
TATACTGGAC CAGAAGTTTC AGATCCAATG AGTTCCACCT ACATAGAGGA ATTGGGTAAA 
AGAGAAGTCA CAATTCCTCC AAAATATAGG GAACTATTGG CTAAACCCAT AGGGCCAGAT 
GATGCTATAG ACGCCTTGTC ATCTGACTTC ACCTGTGGGT CGCCTACAGC TGCTGGAAAG 
AAAACTGAAA AAGAGGAATC TACAGAAGTT TTAAAAGCTC AGTCAGCAGG GACAGTCAGA 
AGTGCTGCTC CACCCCAAGA GAAGAAAAGA AAGGTGGAGA AGGATACAAT GAGTGATCAA 
GCACTCGAGG CTCTGTCGGC TTCACTGGGC ACCCGGCAAG CAGAACCTGA GCTCGACCTC 
CGCTCAATTA AGGAAGTCGA TGAGGATAAA GATGGAAAAC CACTATTGCC AGAGCCTGAA 
GAAAAACCCA AGCCTCGGAG TGAATCAGAA CTCATTGATG AACTTTCAGA AGATTTTGAC 
CGGTCTGAAT GTAAAGAGAA ACCATCTAAG CCAACTGAAA AGACAGAAGA ATCTAAGGCC 
GCTGCTCCAG CTCCTGTGTC GGAGGCTGTG TGTCGGACCT CCATGTGTAG TATACAGTCA 
GCACCCCCTG AGCCGGCTAC CTTGAAGGGC ACAGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGATAGCC TGGGGAAAAA GGAAGCAGAT CCAGAAGATG GAAAACCTGT GATGGATAAA 
GTCAAGGAGA AGGCCAAAGA AGAAGACCGT GAAAAGCTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAGCCACT CCTGCCAAAA 
GAGTCTAAGG AACAGCTTCC ACCCATGAGT GAAGACTTCC TTCTGGATGC TTTGTCTGAG 
GACTTCTCTG GTCCACAAAA TGCTTCATCT CTTAAATTTG AAGATGCTAA ACTTGCTGCT 
GCCATCTCTG AAGTGGTTTC CCAAACCCCA GCTTCAACGA CCCAAGCTGG AGCCCCACCC 
CGTGATACCT CGAGTGACAA AGACCTCGAT GATGCCTTGG ATAAACTCTC TGACAGTCTA 
GGACAAAGGC AGCCTGACCC AGATGAGAAC AAACCAATGG AAGATAAAGT AAAGGAAAAA 
GCTAAAGCTG AACATAGAGA CAAGCTTGGA GAAAGAGATG ACACTATCCC ACCTGAATAC 
AGACATCTCC TGGATGATAA TGGACAGGAC AAACCAGTGA AGCCACCTAC AAAGAAATCA 
GAGGATTCAA AGAAACCTGC AGATGACCAA GACCCCATTG ATGCTCTCTC AGGAGATCTG 
GACAGCTGTC CCTCCACTAC AGAAACCTCA CAGAACACAG CAAAGGATAA GTGCAAGAAG 
GCTGCTTCCA GCTCCAAAGC ACCTAAGAAT GGAGGTAAAG CGAAGGATTC AGCAAAGACA 
ACAGAGGAAA CTTCCAAGCC AAAAGATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KSHV reduces autophagy in THP-1 cells and in differentiating monocytes by decreasing CAST/calpastatin and ATG5 expression.
Autophagy12(12)2311-2325(2016 Dec)
Santarelli R,Granato M,Pentassuglia G,Lacconi V,Gilardini Montani MS,Gonnella R,Tafani M,Torrisi MR,Faggioni A,Cirone M

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CAST cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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