cast cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following cast gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cast cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa31736 XM_012956101.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $510.30
$729.00
OXa31732 XM_012956074.2
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31733 XM_012956081.2
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31738 XM_012956113.2
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47520 XM_031895384.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47521 XM_031895388.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47522 XM_031895397.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47523 XM_031895399.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47524 XM_031895403.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47525 XM_031895407.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47526 XM_031895410.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47527 XM_031895413.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47528 XM_031895421.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa47529 XM_031895424.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31725 XM_012956017.2
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47517 XM_031895367.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47517 XM_031895362.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47517 XM_031895374.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47517 XM_031895373.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47518 XM_031895377.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa47519 XM_031895380.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa21874 NM_001126837.1
Latest version!
Xenopus tropicalis calpastatin (cast), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31725 XM_012956023.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OXa31725 XM_012956026.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OXa31726 XM_012956032.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OXa31727 XM_012956040.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OXa31728 XM_012956046.2
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31729 XM_012956054.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31731 XM_012956066.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31734 XM_012956088.2
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31735 XM_012956093.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31737 XM_012956106.2
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31739 XM_012956118.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31740 XM_012956125.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31741 XM_012956133.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa41680 XM_018090942.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa41681 XM_018090946.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa41682 XM_018090966.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa41683 XM_018090967.1
Latest version!
Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
View Old Accession Versions >>
OXa31733 XM_012956081.1 Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31738 XM_012956113.1 Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $510.30
$729.00
OXa31732 XM_012956074.1 Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OXa31725 XM_012956017.1 Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa31736
    Clone ID Related Accession (Same CDS sequence) XM_012956101.1
    Accession Version XM_012956101.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2904bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT
    CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA
    GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG
    CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT
    CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG
    GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA
    CTAGATGGGA AAGTAGATGG AAAAGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG
    ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA
    ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA
    GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA
    GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA
    CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA
    GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG
    GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG
    GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC
    CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT
    AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC
    GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA
    AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG
    GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT
    AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT
    GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT
    GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG
    TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT
    GTGCCAAAGG CTGGCAAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811555.1
    CDS206..3109
    Translation

    Target ORF information:

    RefSeq Version XM_012956101.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956101.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT
    CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA
    GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG
    CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT
    CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG
    GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA
    CTAGATGGGA AAGTAGATGG AAAAGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG
    ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA
    ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA
    GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA
    GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA
    CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA
    GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG
    GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG
    GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC
    CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT
    AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC
    GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA
    AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG
    GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT
    AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT
    GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT
    GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG
    TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT
    GTGCCAAAGG CTGGCAAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31738
    Clone ID Related Accession (Same CDS sequence) XM_012956113.2 , XM_012956113.1
    Accession Version XM_012956113.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAGAAAC TCCTCAAGGT
    AACAAAACGA AACCTGAAAT TGCGGCATCA TCTGCATCCA CTGTAAAAGC AGCAGCGCCC
    TCCGGTGGTA TGGACAGTGC ATTAGATGAA TTATTGGGAA CACTGGAAGG TCCTGATCCA
    ACAGCCCCTG AATCACCTGT GTACACTGGG CCAGAGGTGA CGGAAACAGA GACAGCGGTA
    TACAGAGAGG GACTTGGGAT AAGAGACAGC AGCATCCCTC CAGCATACAG GCACTTACTA
    GATGGGAAAG TAGATGGAAA AGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC
    GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA
    CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA
    GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA
    CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG
    AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA
    AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT
    AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC
    GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA
    CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA
    GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC
    GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC
    AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG
    GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG
    GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC
    GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA
    CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG
    CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG
    CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811567.1
    CDS206..3046
    Translation

    Target ORF information:

    RefSeq Version XM_012956113.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956113.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAGAAAC TCCTCAAGGT
    AACAAAACGA AACCTGAAAT TGCGGCATCA TCTGCATCCA CTGTAAAAGC AGCAGCGCCC
    TCCGGTGGTA TGGACAGTGC ATTAGATGAA TTATTGGGAA CACTGGAAGG TCCTGATCCA
    ACAGCCCCTG AATCACCTGT GTACACTGGG CCAGAGGTGA CGGAAACAGA GACAGCGGTA
    TACAGAGAGG GACTTGGGAT AAGAGACAGC AGCATCCCTC CAGCATACAG GCACTTACTA
    GATGGGAAAG TAGATGGAAA AGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC
    GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA
    CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA
    GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA
    CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG
    AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA
    AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT
    AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC
    GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA
    CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA
    GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC
    GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC
    AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG
    GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG
    GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC
    GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA
    CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG
    CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG
    CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31732
    Clone ID Related Accession (Same CDS sequence) XM_012956074.2 , XM_012956074.1
    Accession Version XM_012956074.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811528.1
    CDS205..3150
    Translation

    Target ORF information:

    RefSeq Version XM_012956074.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956074.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31733
    Clone ID Related Accession (Same CDS sequence) XM_012956081.1 , XM_012956081.2
    Accession Version XM_012956081.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811535.1
    CDS205..3147
    Translation

    Target ORF information:

    RefSeq Version XM_012956081.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31738
    Clone ID Related Accession (Same CDS sequence) XM_012956113.2 , XM_012956113.1
    Accession Version XM_012956113.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAGAAAC TCCTCAAGGT
    AACAAAACGA AACCTGAAAT TGCGGCATCA TCTGCATCCA CTGTAAAAGC AGCAGCGCCC
    TCCGGTGGTA TGGACAGTGC ATTAGATGAA TTATTGGGAA CACTGGAAGG TCCTGATCCA
    ACAGCCCCTG AATCACCTGT GTACACTGGG CCAGAGGTGA CGGAAACAGA GACAGCGGTA
    TACAGAGAGG GACTTGGGAT AAGAGACAGC AGCATCCCTC CAGCATACAG GCACTTACTA
    GATGGGAAAG TAGATGGAAA AGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC
    GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA
    CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA
    GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA
    CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG
    AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA
    AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT
    AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC
    GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA
    CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA
    GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC
    GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC
    AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG
    GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG
    GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC
    GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA
    CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG
    CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG
    CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811567.1
    CDS205..3045
    Translation

    Target ORF information:

    RefSeq Version XM_012956113.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956113.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAGAAAC TCCTCAAGGT
    AACAAAACGA AACCTGAAAT TGCGGCATCA TCTGCATCCA CTGTAAAAGC AGCAGCGCCC
    TCCGGTGGTA TGGACAGTGC ATTAGATGAA TTATTGGGAA CACTGGAAGG TCCTGATCCA
    ACAGCCCCTG AATCACCTGT GTACACTGGG CCAGAGGTGA CGGAAACAGA GACAGCGGTA
    TACAGAGAGG GACTTGGGAT AAGAGACAGC AGCATCCCTC CAGCATACAG GCACTTACTA
    GATGGGAAAG TAGATGGAAA AGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC
    GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA
    CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA
    GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA
    CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG
    AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA
    AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT
    AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC
    GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA
    CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA
    GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC
    GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC
    AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG
    GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG
    GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC
    GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA
    CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG
    CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG
    CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47520
    Clone ID Related Accession (Same CDS sequence) XM_031895384.1
    Accession Version XM_031895384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
    CCATCAGAAG GAGCAGAGAA GAAAACTGGT GCCAGTGGCC CTTCAGGGGC CCAAGCAAGT
    GGGACCAGCA AGACTCCTAG TGCTTCAGGA AAAACTATGA ACCCGGCGGA TACACAGGCT
    AAACAAACAC CCAAGACTGA ACCAGCCAAA TCTACAAAGC CAACAAAGCC AAAAGAAGCA
    ACAACAGCAT CTTCAGCAGC CCTGGCGGCT GCATCCAAAC CAACAGCAGC ACCTTCCTCA
    GCCCCAGTAA CAGCAACCAA ACCAACAAAA ACAAAGCCAA GTGCAGCAAA ATCGCCTCCC
    AAACAAGATA TAAAGCCTAT CCCTGGGAAG CAGCAAAAAA CAGACAGCAA GAGTAAAGAT
    GAAAAGAAAA GTGGTGATAA AGTTTCTCCA GCTGTGCTAC CTAAGATGAC ACCTAGTTCT
    GCTGGTAAAG TATCTGCAGT TGCCACTGGA GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA
    GTCACTGCTG TGCAGTCAAC AAAGCCGAAA GATGAGAGTA AAGAGGAAAA GAAAGCCATT
    ACTGGGGATA AGGTTTCTTC TGGGCCACCG GCCAAGACAG ACGCAGATGC AGCCTTGGAC
    GAACTACTTG GAACGTTAGA GGGCCCAGCA GATATTCCAG AGTCTCCACA ATTTACTGGA
    CCAGAAATCC AGGACACAGT TGTTACATCT GAGTATTTGG AGGAGCTAGG AAAACGGGAT
    TTCACCATCC CACCTGAATA CAGGCACCTG CTGGATGGTA AAGGTGAAAA GATGGCACCC
    CCTACCCCAC CCGAGGAACC CGAAGAGCAC ATGGATGATG ATGAATTAGT TGCAGCCCTG
    TCTTCTGGTT TTGAGAGCAG TCAGGCATCC CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG
    AAAATGGAAG TGAAACCAAA ATCAGCAGCA GCAGTGGCCA CACAGCCAGA TGTGCAGAAA
    AAAGAAGTAA AAGTCGCAGC AGCAGAAACT TCCAAACCAA GCACTCAGAA AAAAGAGGAA
    AAAGCAAAAA TAGCAGAAGT GTCTAAACCA AGCACTCAGA AAAAAGAGGA AAAACTGGCA
    GCAGCAGCAG ACACATCCAA ACCAAGCACT CAGAAAAAAG AGGTAAAAGC CACAGCAAAA
    GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC
    GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGGGTCTCC AGTATTTCAG
    GGTCCAGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT
    AGTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG
    TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CAGAGCTTGT AGATGTGTTT
    TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC
    AAAAGGAACA CTGAAGATCT CCACGAGTGC TATTGCTCAA AGTCAGTGAG CCAACTTTCA
    TCATCTGAAA CTCCTCAAGG TAACAAAACG AAACCTGAAA TTGCGGCATC ATCTGCATCC
    ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT ATGGACAGTG CATTAGATGA ATTATTGGGA
    ACACTGGAAG GTCCTGATCC AACAGCCCCT GAATCACCTG TGTACACTGG GCCAGAGGTG
    ACGGAAACAG AGACAGCGGT ATACAGAGAG GGACTTGGGA TAAGAGACAG CAGCATCCCT
    CCAGCATACA GGCACTTACT AGATGATTCT GCTGGAGGAG CACTATTAAC TGATGCACCC
    CCACCACCGC CAGAGGAAAA GCCTCTGACC GATGATCAGC TGATTGATAA GTTTTCTATG
    GACTTTGATT GTTCTGAATC TCCTGCAACA CAACAGGCTC ATCCTGTCGA ACCTAAAGTT
    TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA GTAGTTGCTG CCTCCTCTGC TACTGTTAAA
    GCTGCACCCA CTCCTTCTAA AGCACCAGCA CCTCCAGCTG ATCCACTCGA TAGCCTGTCT
    GGTACCTTAG GTGTTCGAAA GGAAGAACCG AGTGACAAGA AGCCTCCAGT GGATAAAGTA
    AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA AAGCTTGGAG AAGATGAAAA AACAATACAT
    CCTGACCGCC GGCTAAAGGA AGTGAAGGAT AAAGACGGAA AGCCCTTGCT GCCAAAACTT
    GAACAAAGCC CTAAGCCAAT GAGTGAGGCC GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT
    GTGACTTCCC CTGCCACTCC TGATGCCCCA CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT
    GGATCAGAAG ATGTTATATC TTGTTCTAAA GCTTCCGCCG TCAAGTCTGG AGCTCCACAG
    GACGCTGCTT TAGATATACA GATTCCCGAC GATGCACTAG ATTTACTTTC TGGCTCCCTG
    GGACAAAGAG AGGTGGATCC TAATGAAAAC AAACCGGTGG TGGATGTAGT TAAGGAAAAA
    GCTAAAGCTG AGCATATAGA CAAACTGGGG GACAGAGATG ATACAATCCC ACCGGAATAC
    AGAAAGCTGC TGGATGGTAA AGATGATAAG GGACAACCTG CAAAACCACC AGCGAAAGCG
    GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC GATGCAGCGA TCGATGCTCT TTCAAGCGGA
    TTTGCATCAT GTGACACAAA ATCTACTGAA CAACCAAAGC AGCCTTCAAC GGACAAGACT
    GAGAAACAAT GTGCTAGCGC TCCAGTGTCG CAGCCTTCAG GAAAGGCACC TGATGCTGCA
    AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751244.1
    CDS152..3142
    Translation

    Target ORF information:

    RefSeq Version XM_031895384.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
    CCATCAGAAG GAGCAGAGAA GAAAACTGGT GCCAGTGGCC CTTCAGGGGC CCAAGCAAGT
    GGGACCAGCA AGACTCCTAG TGCTTCAGGA AAAACTATGA ACCCGGCGGA TACACAGGCT
    AAACAAACAC CCAAGACTGA ACCAGCCAAA TCTACAAAGC CAACAAAGCC AAAAGAAGCA
    ACAACAGCAT CTTCAGCAGC CCTGGCGGCT GCATCCAAAC CAACAGCAGC ACCTTCCTCA
    GCCCCAGTAA CAGCAACCAA ACCAACAAAA ACAAAGCCAA GTGCAGCAAA ATCGCCTCCC
    AAACAAGATA TAAAGCCTAT CCCTGGGAAG CAGCAAAAAA CAGACAGCAA GAGTAAAGAT
    GAAAAGAAAA GTGGTGATAA AGTTTCTCCA GCTGTGCTAC CTAAGATGAC ACCTAGTTCT
    GCTGGTAAAG TATCTGCAGT TGCCACTGGA GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA
    GTCACTGCTG TGCAGTCAAC AAAGCCGAAA GATGAGAGTA AAGAGGAAAA GAAAGCCATT
    ACTGGGGATA AGGTTTCTTC TGGGCCACCG GCCAAGACAG ACGCAGATGC AGCCTTGGAC
    GAACTACTTG GAACGTTAGA GGGCCCAGCA GATATTCCAG AGTCTCCACA ATTTACTGGA
    CCAGAAATCC AGGACACAGT TGTTACATCT GAGTATTTGG AGGAGCTAGG AAAACGGGAT
    TTCACCATCC CACCTGAATA CAGGCACCTG CTGGATGGTA AAGGTGAAAA GATGGCACCC
    CCTACCCCAC CCGAGGAACC CGAAGAGCAC ATGGATGATG ATGAATTAGT TGCAGCCCTG
    TCTTCTGGTT TTGAGAGCAG TCAGGCATCC CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG
    AAAATGGAAG TGAAACCAAA ATCAGCAGCA GCAGTGGCCA CACAGCCAGA TGTGCAGAAA
    AAAGAAGTAA AAGTCGCAGC AGCAGAAACT TCCAAACCAA GCACTCAGAA AAAAGAGGAA
    AAAGCAAAAA TAGCAGAAGT GTCTAAACCA AGCACTCAGA AAAAAGAGGA AAAACTGGCA
    GCAGCAGCAG ACACATCCAA ACCAAGCACT CAGAAAAAAG AGGTAAAAGC CACAGCAAAA
    GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC
    GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGGGTCTCC AGTATTTCAG
    GGTCCAGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT
    AGTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG
    TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CAGAGCTTGT AGATGTGTTT
    TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC
    AAAAGGAACA CTGAAGATCT CCACGAGTGC TATTGCTCAA AGTCAGTGAG CCAACTTTCA
    TCATCTGAAA CTCCTCAAGG TAACAAAACG AAACCTGAAA TTGCGGCATC ATCTGCATCC
    ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT ATGGACAGTG CATTAGATGA ATTATTGGGA
    ACACTGGAAG GTCCTGATCC AACAGCCCCT GAATCACCTG TGTACACTGG GCCAGAGGTG
    ACGGAAACAG AGACAGCGGT ATACAGAGAG GGACTTGGGA TAAGAGACAG CAGCATCCCT
    CCAGCATACA GGCACTTACT AGATGATTCT GCTGGAGGAG CACTATTAAC TGATGCACCC
    CCACCACCGC CAGAGGAAAA GCCTCTGACC GATGATCAGC TGATTGATAA GTTTTCTATG
    GACTTTGATT GTTCTGAATC TCCTGCAACA CAACAGGCTC ATCCTGTCGA ACCTAAAGTT
    TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA GTAGTTGCTG CCTCCTCTGC TACTGTTAAA
    GCTGCACCCA CTCCTTCTAA AGCACCAGCA CCTCCAGCTG ATCCACTCGA TAGCCTGTCT
    GGTACCTTAG GTGTTCGAAA GGAAGAACCG AGTGACAAGA AGCCTCCAGT GGATAAAGTA
    AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA AAGCTTGGAG AAGATGAAAA AACAATACAT
    CCTGACCGCC GGCTAAAGGA AGTGAAGGAT AAAGACGGAA AGCCCTTGCT GCCAAAACTT
    GAACAAAGCC CTAAGCCAAT GAGTGAGGCC GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT
    GTGACTTCCC CTGCCACTCC TGATGCCCCA CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT
    GGATCAGAAG ATGTTATATC TTGTTCTAAA GCTTCCGCCG TCAAGTCTGG AGCTCCACAG
    GACGCTGCTT TAGATATACA GATTCCCGAC GATGCACTAG ATTTACTTTC TGGCTCCCTG
    GGACAAAGAG AGGTGGATCC TAATGAAAAC AAACCGGTGG TGGATGTAGT TAAGGAAAAA
    GCTAAAGCTG AGCATATAGA CAAACTGGGG GACAGAGATG ATACAATCCC ACCGGAATAC
    AGAAAGCTGC TGGATGGTAA AGATGATAAG GGACAACCTG CAAAACCACC AGCGAAAGCG
    GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC GATGCAGCGA TCGATGCTCT TTCAAGCGGA
    TTTGCATCAT GTGACACAAA ATCTACTGAA CAACCAAAGC AGCCTTCAAC GGACAAGACT
    GAGAAACAAT GTGCTAGCGC TCCAGTGTCG CAGCCTTCAG GAAAGGCACC TGATGCTGCA
    AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47521
    Clone ID Related Accession (Same CDS sequence) XM_031895388.1
    Accession Version XM_031895388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA
    AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA
    CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA
    CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT
    ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA
    AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA
    CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT
    TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC
    GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC
    AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC
    GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA
    GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC
    AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT
    GATTCTGCTG GAGGAGCACT ATTAACTGAT GCACCCCCAC CACCGCCAGA GGAAAAGCCT
    CTGACCGATG ATCAGCTGAT TGATAAGTTT TCTATGGACT TTGATTGTTC TGAATCTCCT
    GCAACACAAC AGGCTCATCC TGTCGAACCT AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA
    AAAGAAGTAG TTGCTGCCTC CTCTGCTACT GTTAAAGCTG CACCCACTCC TTCTAAAGCA
    CCAGCACCTC CAGCTGATCC ACTCGATAGC CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA
    GAACCGAGTG ACAAGAAGCC TCCAGTGGAT AAAGTAAAGG AGGGAACAGG CAAAGAGAAG
    AAAGAAAAGC TTGGAGAAGA TGAAAAAACA ATACATCCTG ACCGCCGGCT AAAGGAAGTG
    AAGGATAAAG ACGGAAAGCC CTTGCTGCCA AAACTTGAAC AAAGCCCTAA GCCAATGAGT
    GAGGCCGAGC TGCTGGATGC TTTGACTGAA GGCTTTGTGA CTTCCCCTGC CACTCCTGAT
    GCCCCACTGC AGGGCTCTTT GAAAAAAAAA GGTTCTGGAT CAGAAGATGT TATATCTTGT
    TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT CCACAGGACG CTGCTTTAGA TATACAGATT
    CCCGACGATG CACTAGATTT ACTTTCTGGC TCCCTGGGAC AAAGAGAGGT GGATCCTAAT
    GAAAACAAAC CGGTGGTGGA TGTAGTTAAG GAAAAAGCTA AAGCTGAGCA TATAGACAAA
    CTGGGGGACA GAGATGATAC AATCCCACCG GAATACAGAA AGCTGCTGGA TGGTAAAGAT
    GATAAGGGAC AACCTGCAAA ACCACCAGCG AAAGCGGAGG AGAAACCAAA GAAGCCTTTA
    AGTGACGATG CAGCGATCGA TGCTCTTTCA AGCGGATTTG CATCATGTGA CACAAAATCT
    ACTGAACAAC CAAAGCAGCC TTCAACGGAC AAGACTGAGA AACAATGTGC TAGCGCTCCA
    GTGTCGCAGC CTTCAGGAAA GGCACCTGAT GCTGCAAAGC AGGCAAACTC AGCGATACCA
    AGTGTGCCAA AGGCTGGCAA AAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751248.1
    CDS205..3171
    Translation

    Target ORF information:

    RefSeq Version XM_031895388.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA
    AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA
    CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA
    CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT
    ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA
    AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA
    CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT
    TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC
    GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC
    AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC
    GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA
    GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC
    AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT
    GATTCTGCTG GAGGAGCACT ATTAACTGAT GCACCCCCAC CACCGCCAGA GGAAAAGCCT
    CTGACCGATG ATCAGCTGAT TGATAAGTTT TCTATGGACT TTGATTGTTC TGAATCTCCT
    GCAACACAAC AGGCTCATCC TGTCGAACCT AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA
    AAAGAAGTAG TTGCTGCCTC CTCTGCTACT GTTAAAGCTG CACCCACTCC TTCTAAAGCA
    CCAGCACCTC CAGCTGATCC ACTCGATAGC CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA
    GAACCGAGTG ACAAGAAGCC TCCAGTGGAT AAAGTAAAGG AGGGAACAGG CAAAGAGAAG
    AAAGAAAAGC TTGGAGAAGA TGAAAAAACA ATACATCCTG ACCGCCGGCT AAAGGAAGTG
    AAGGATAAAG ACGGAAAGCC CTTGCTGCCA AAACTTGAAC AAAGCCCTAA GCCAATGAGT
    GAGGCCGAGC TGCTGGATGC TTTGACTGAA GGCTTTGTGA CTTCCCCTGC CACTCCTGAT
    GCCCCACTGC AGGGCTCTTT GAAAAAAAAA GGTTCTGGAT CAGAAGATGT TATATCTTGT
    TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT CCACAGGACG CTGCTTTAGA TATACAGATT
    CCCGACGATG CACTAGATTT ACTTTCTGGC TCCCTGGGAC AAAGAGAGGT GGATCCTAAT
    GAAAACAAAC CGGTGGTGGA TGTAGTTAAG GAAAAAGCTA AAGCTGAGCA TATAGACAAA
    CTGGGGGACA GAGATGATAC AATCCCACCG GAATACAGAA AGCTGCTGGA TGGTAAAGAT
    GATAAGGGAC AACCTGCAAA ACCACCAGCG AAAGCGGAGG AGAAACCAAA GAAGCCTTTA
    AGTGACGATG CAGCGATCGA TGCTCTTTCA AGCGGATTTG CATCATGTGA CACAAAATCT
    ACTGAACAAC CAAAGCAGCC TTCAACGGAC AAGACTGAGA AACAATGTGC TAGCGCTCCA
    GTGTCGCAGC CTTCAGGAAA GGCACCTGAT GCTGCAAAGC AGGCAAACTC AGCGATACCA
    AGTGTGCCAA AGGCTGGCAA AAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47522
    Clone ID Related Accession (Same CDS sequence) XM_031895397.1
    Accession Version XM_031895397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC
    AAATCTACAA AGCCAACAAA GCCAAAAGAA GCAACAACAG CATCTTCAGC AGCCCTGGCG
    GCTGCATCCA AACCAACAGC AGCACCTTCC TCAGCCCCAG TAACAGCAAC CAAACCAACA
    AAAACAAAGC CAAGTGCAGC AAAATCGCCT CCCAAACAAG ATATAAAGCC TATCCCTGGG
    AAGCAGCAAA AAACAGACAG CAAGAGTAAA GATGAAAAGA AAAGTGGTGA TAAAGTTTCT
    CCAGCTGTGC TACCTAAGAT GACACCTAGT TCTGCTGGTA AAGTATCTGC AGTTGCCACT
    GGAGCTCTAG CAGCTGCTGG TGCCGCCGCT GCAGTCACTG CTGTGCAGTC AACAAAGCCG
    AAAGATGAGA GTAAAGAGGA AAAGAAAGCC ATTACTGGGG ATAAGGTTTC TTCTGGGCCA
    CCGGCCAAGA CAGACGCAGA TGCAGCCTTG GACGAACTAC TTGGAACGTT AGAGGGCCCA
    GCAGATATTC CAGAGTCTCC ACAATTTACT GGACCAGAAA TCCAGGACAC AGTTGTTACA
    TCTGAGTATT TGGAGGAGCT AGGAAAACGG GATTTCACCA TCCCACCTGA ATACAGGCAC
    CTGCTGGATG GTAAAGGTGA AAAGATGGCA CCCCCTACCC CACCCGAGGA ACCCGAAGAG
    CACATGGATG ATGATGAATT AGTTGCAGCC CTGTCTTCTG GTTTTGAGAG CAGTCAGGCA
    TCCCCCGAAG TAAAGAAGCC AAAGCTGGAA GAGAAAATGG AAGTGAAACC AAAATCAGCA
    GCAGCAGTGG CCACACAGCC AGATGTGCAG AAAAAAGAAG TAAAAGTCGC AGCAGCAGAA
    ACTTCCAAAC CAAGCACTCA GAAAAAAGAG GAAAAAGCAA AAATAGCAGA AGTGTCTAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAACTG GCAGCAGCAG CAGACACATC CAAACCAAGC
    ACTCAGAAAA AAGAGGTAAA AGCCACAGCA AAAGCCACAG CTGCCGAAGC TTCCAAACAA
    AGCACTGAGT CCATACCATC AGAAGCAATA GACGAGTTGT TGGGAACACT TGAAGGACCC
    CCAGAAAATG TTCCAGGGTC TCCAGTATTT CAGGGTCCAG AAGTTACTGA GAACATTACA
    GCAACATACT TAGAAGAAAC TGGAAAAAGA GATAGTACGA TCCCTCCAGA ATACAGAAAA
    TTATTAGATG GAAAGGATCA TGGGAAAGAT GTGTGGCCTT CAGCACCTGA ACCAGAACCG
    TCTATGACCG ACACAGAGCT TGTAGATGTG TTTTCCAAAG ACTTTGAGCT TTCATGTTCA
    CCTACTGTTC AGACCACGCC AGCCATTAAA CCCAAAAGGA ACACTGAAGA TCTCCACGAG
    TGCTATTGCT CAAAGTCAGT GAGCCAACTT TCATCATCTG AAACTCCTCA AGGTAACAAA
    ACGAAACCTG AAATTGCGGC ATCATCTGCA TCCACTGTAA AAGCAGCAGC GCCCTCCGGT
    GGTATGGACA GTGCATTAGA TGAATTATTG GGAACACTGG AAGGTCCTGA TCCAACAGCC
    CCTGAATCAC CTGTGTACAC TGGGCCAGAG GTGACGGAAA CAGAGACAGC GGTATACAGA
    GAGGGACTTG GGATAAGAGA CAGCAGCATC CCTCCAGCAT ACAGGCACTT ACTAGATGAT
    TCTGCTGGAG GAGCACTATT AACTGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG
    ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA
    ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA
    GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA
    GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA
    CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA
    GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG
    GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG
    GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC
    CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT
    AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC
    GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA
    AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG
    GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT
    AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT
    GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT
    GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG
    TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT
    GTGCCAAAGG CTGGCAAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751257.1
    CDS205..3168
    Translation

    Target ORF information:

    RefSeq Version XM_031895397.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC
    AAATCTACAA AGCCAACAAA GCCAAAAGAA GCAACAACAG CATCTTCAGC AGCCCTGGCG
    GCTGCATCCA AACCAACAGC AGCACCTTCC TCAGCCCCAG TAACAGCAAC CAAACCAACA
    AAAACAAAGC CAAGTGCAGC AAAATCGCCT CCCAAACAAG ATATAAAGCC TATCCCTGGG
    AAGCAGCAAA AAACAGACAG CAAGAGTAAA GATGAAAAGA AAAGTGGTGA TAAAGTTTCT
    CCAGCTGTGC TACCTAAGAT GACACCTAGT TCTGCTGGTA AAGTATCTGC AGTTGCCACT
    GGAGCTCTAG CAGCTGCTGG TGCCGCCGCT GCAGTCACTG CTGTGCAGTC AACAAAGCCG
    AAAGATGAGA GTAAAGAGGA AAAGAAAGCC ATTACTGGGG ATAAGGTTTC TTCTGGGCCA
    CCGGCCAAGA CAGACGCAGA TGCAGCCTTG GACGAACTAC TTGGAACGTT AGAGGGCCCA
    GCAGATATTC CAGAGTCTCC ACAATTTACT GGACCAGAAA TCCAGGACAC AGTTGTTACA
    TCTGAGTATT TGGAGGAGCT AGGAAAACGG GATTTCACCA TCCCACCTGA ATACAGGCAC
    CTGCTGGATG GTAAAGGTGA AAAGATGGCA CCCCCTACCC CACCCGAGGA ACCCGAAGAG
    CACATGGATG ATGATGAATT AGTTGCAGCC CTGTCTTCTG GTTTTGAGAG CAGTCAGGCA
    TCCCCCGAAG TAAAGAAGCC AAAGCTGGAA GAGAAAATGG AAGTGAAACC AAAATCAGCA
    GCAGCAGTGG CCACACAGCC AGATGTGCAG AAAAAAGAAG TAAAAGTCGC AGCAGCAGAA
    ACTTCCAAAC CAAGCACTCA GAAAAAAGAG GAAAAAGCAA AAATAGCAGA AGTGTCTAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAACTG GCAGCAGCAG CAGACACATC CAAACCAAGC
    ACTCAGAAAA AAGAGGTAAA AGCCACAGCA AAAGCCACAG CTGCCGAAGC TTCCAAACAA
    AGCACTGAGT CCATACCATC AGAAGCAATA GACGAGTTGT TGGGAACACT TGAAGGACCC
    CCAGAAAATG TTCCAGGGTC TCCAGTATTT CAGGGTCCAG AAGTTACTGA GAACATTACA
    GCAACATACT TAGAAGAAAC TGGAAAAAGA GATAGTACGA TCCCTCCAGA ATACAGAAAA
    TTATTAGATG GAAAGGATCA TGGGAAAGAT GTGTGGCCTT CAGCACCTGA ACCAGAACCG
    TCTATGACCG ACACAGAGCT TGTAGATGTG TTTTCCAAAG ACTTTGAGCT TTCATGTTCA
    CCTACTGTTC AGACCACGCC AGCCATTAAA CCCAAAAGGA ACACTGAAGA TCTCCACGAG
    TGCTATTGCT CAAAGTCAGT GAGCCAACTT TCATCATCTG AAACTCCTCA AGGTAACAAA
    ACGAAACCTG AAATTGCGGC ATCATCTGCA TCCACTGTAA AAGCAGCAGC GCCCTCCGGT
    GGTATGGACA GTGCATTAGA TGAATTATTG GGAACACTGG AAGGTCCTGA TCCAACAGCC
    CCTGAATCAC CTGTGTACAC TGGGCCAGAG GTGACGGAAA CAGAGACAGC GGTATACAGA
    GAGGGACTTG GGATAAGAGA CAGCAGCATC CCTCCAGCAT ACAGGCACTT ACTAGATGAT
    TCTGCTGGAG GAGCACTATT AACTGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG
    ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA
    ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA
    GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA
    GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA
    CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA
    GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG
    GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG
    GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC
    CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT
    AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC
    GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA
    AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG
    GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT
    AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT
    GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT
    GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG
    TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT
    GTGCCAAAGG CTGGCAAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47523
    Clone ID Related Accession (Same CDS sequence) XM_031895399.1
    Accession Version XM_031895399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGA TTCTGCTGGA
    GGAGCACTAT TAACTGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT
    CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG
    GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT
    GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA
    GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC
    AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT
    GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC
    GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG
    CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG
    GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC
    GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA
    CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG
    GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA
    GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA
    CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA
    GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA
    AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT
    TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG
    GCTGGCAAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751259.1
    CDS205..3159
    Translation

    Target ORF information:

    RefSeq Version XM_031895399.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGA TTCTGCTGGA
    GGAGCACTAT TAACTGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT
    CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG
    GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT
    GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA
    GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC
    AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT
    GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC
    GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG
    CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG
    GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC
    GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA
    CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG
    GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA
    GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA
    CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA
    GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA
    AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT
    TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG
    GCTGGCAAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47524
    Clone ID Related Accession (Same CDS sequence) XM_031895403.1
    Accession Version XM_031895403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
    CCATCAGAAG GAGCAGAGAC TCCTAGTGCT TCAGGAAAAA CTATGAACCC GGCGGATACA
    CAGGCTAAAC AAACACCCAA GACTGAACCA GCCAAATCTA CAAAGCCAAC AAAGCCAAAA
    GAAGCAACAA CAGCATCTTC AGCAGCCCTG GCGGCTGCAT CCAAACCAAC AGCAGCACCT
    TCCTCAGCCC CAGTAACAGC AACCAAACCA ACAAAAACAA AGCCAAGTGC AGCAAAATCG
    CCTCCCAAAC AAGATATAAA GCCTATCCCT GGGAAGCAGC AAAAAACAGA CAGCAAGAGT
    AAAGATGAAA AGAAAAGTGG TGATAAAGTT TCTCCAGCTG TGCTACCTAA GATGACACCT
    AGTTCTGCTG GTAAAGTATC TGCAGTTGCC ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC
    GCTGCAGTCA CTGCTGTGCA GTCAACAAAG CCGAAAGATG AGAGTAAAGA GGAAAAGAAA
    GCCATTACTG GGGATAAGGT TTCTTCTGGG CCACCGGCCA AGACAGACGC AGATGCAGCC
    TTGGACGAAC TACTTGGAAC GTTAGAGGGC CCAGCAGATA TTCCAGAGTC TCCACAATTT
    ACTGGACCAG AAATCCAGGA CACAGTTGTT ACATCTGAGT ATTTGGAGGA GCTAGGAAAA
    CGGGATTTCA CCATCCCACC TGAATACAGG CACCTGCTGG ATGGTAAAGG TGAAAAGATG
    GCACCCCCTA CCCCACCCGA GGAACCCGAA GAGCACATGG ATGATGATGA ATTAGTTGCA
    GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG GCATCCCCCG AAGTAAAGAA GCCAAAGCTG
    GAAGAGAAAA TGGAAGTGAA ACCAAAATCA GCAGCAGCAG TGGCCACACA GCCAGATGTG
    CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA GAAACTTCCA AACCAAGCAC TCAGAAAAAA
    GAGGAAAAAG CAAAAATAGC AGAAGTGTCT AAACCAAGCA CTCAGAAAAA AGAGGAAAAA
    CTGGCAGCAG CAGCAGACAC ATCCAAACCA AGCACTCAGA AAAAAGAGGT AAAAGCCACA
    GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA CAAAGCACTG AGTCCATACC ATCAGAAGCA
    ATAGACGAGT TGTTGGGAAC ACTTGAAGGA CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA
    TTTCAGGGTC CAGAAGTTAC TGAGAACATT ACAGCAACAT ACTTAGAAGA AACTGGAAAA
    AGAGATAGTA CGATCCCTCC AGAATACAGA AAATTATTAG ATGGAAAGGA TCATGGGAAA
    GATGTGTGGC CTTCAGCACC TGAACCAGAA CCGTCTATGA CCGACACAGA GCTTGTAGAT
    GTGTTTTCCA AAGACTTTGA GCTTTCATGT TCACCTACTG TTCAGACCAC GCCAGCCATT
    AAACCCAAAA GGAACACTGA AGATCTCCAC GAGTGCTATT GCTCAAAGTC AGTGAGCCAA
    CTTTCATCAT CTGAAACTCC TCAAGGTAAC AAAACGAAAC CTGAAATTGC GGCATCATCT
    GCATCCACTG TAAAAGCAGC AGCGCCCTCC GGTGGTATGG ACAGTGCATT AGATGAATTA
    TTGGGAACAC TGGAAGGTCC TGATCCAACA GCCCCTGAAT CACCTGTGTA CACTGGGCCA
    GAGGTGACGG AAACAGAGAC AGCGGTATAC AGAGAGGGAC TTGGGATAAG AGACAGCAGC
    ATCCCTCCAG CATACAGGCA CTTACTAGAT GATTCTGCTG GAGGAGCACT ATTAACTGAT
    GCACCCCCAC CACCGCCAGA GGAAAAGCCT CTGACCGATG ATCAGCTGAT TGATAAGTTT
    TCTATGGACT TTGATTGTTC TGAATCTCCT GCAACACAAC AGGCTCATCC TGTCGAACCT
    AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA AAAGAAGTAG TTGCTGCCTC CTCTGCTACT
    GTTAAAGCTG CACCCACTCC TTCTAAAGCA CCAGCACCTC CAGCTGATCC ACTCGATAGC
    CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA GAACCGAGTG ACAAGAAGCC TCCAGTGGAT
    AAAGTAAAGG AGGGAACAGG CAAAGAGAAG AAAGAAAAGC TTGGAGAAGA TGAAAAAACA
    ATACATCCTG ACCGCCGGCT AAAGGAAGTG AAGGATAAAG ACGGAAAGCC CTTGCTGCCA
    AAACTTGAAC AAAGCCCTAA GCCAATGAGT GAGGCCGAGC TGCTGGATGC TTTGACTGAA
    GGCTTTGTGA CTTCCCCTGC CACTCCTGAT GCCCCACTGC AGGGCTCTTT GAAAAAAAAA
    GGTTCTGGAT CAGAAGATGT TATATCTTGT TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT
    CCACAGGACG CTGCTTTAGA TATACAGATT CCCGACGATG CACTAGATTT ACTTTCTGGC
    TCCCTGGGAC AAAGAGAGGT GGATCCTAAT GAAAACAAAC CGGTGGTGGA TGTAGTTAAG
    GAAAAAGCTA AAGCTGAGCA TATAGACAAA CTGGGGGACA GAGATGATAC AATCCCACCG
    GAATACAGAA AGCTGCTGGA TGGTAAAGAT GATAAGGGAC AACCTGCAAA ACCACCAGCG
    AAAGCGGAGG AGAAACCAAA GAAGCCTTTA AGTGACGATG CAGCGATCGA TGCTCTTTCA
    AGCGGATTTG CATCATGTGA CACAAAATCT ACTGAACAAC CAAAGCAGCC TTCAACGGAC
    AAGACTGAGA AACAATGTGC TAGCGCTCCA GTGTCGCAGC CTTCAGGAAA GGCACCTGAT
    GCTGCAAAGC AGGCAAACTC AGCGATACCA AGTGTGCCAA AGGCTGGCAA AAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751263.1
    CDS146..3082
    Translation

    Target ORF information:

    RefSeq Version XM_031895403.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
    CCATCAGAAG GAGCAGAGAC TCCTAGTGCT TCAGGAAAAA CTATGAACCC GGCGGATACA
    CAGGCTAAAC AAACACCCAA GACTGAACCA GCCAAATCTA CAAAGCCAAC AAAGCCAAAA
    GAAGCAACAA CAGCATCTTC AGCAGCCCTG GCGGCTGCAT CCAAACCAAC AGCAGCACCT
    TCCTCAGCCC CAGTAACAGC AACCAAACCA ACAAAAACAA AGCCAAGTGC AGCAAAATCG
    CCTCCCAAAC AAGATATAAA GCCTATCCCT GGGAAGCAGC AAAAAACAGA CAGCAAGAGT
    AAAGATGAAA AGAAAAGTGG TGATAAAGTT TCTCCAGCTG TGCTACCTAA GATGACACCT
    AGTTCTGCTG GTAAAGTATC TGCAGTTGCC ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC
    GCTGCAGTCA CTGCTGTGCA GTCAACAAAG CCGAAAGATG AGAGTAAAGA GGAAAAGAAA
    GCCATTACTG GGGATAAGGT TTCTTCTGGG CCACCGGCCA AGACAGACGC AGATGCAGCC
    TTGGACGAAC TACTTGGAAC GTTAGAGGGC CCAGCAGATA TTCCAGAGTC TCCACAATTT
    ACTGGACCAG AAATCCAGGA CACAGTTGTT ACATCTGAGT ATTTGGAGGA GCTAGGAAAA
    CGGGATTTCA CCATCCCACC TGAATACAGG CACCTGCTGG ATGGTAAAGG TGAAAAGATG
    GCACCCCCTA CCCCACCCGA GGAACCCGAA GAGCACATGG ATGATGATGA ATTAGTTGCA
    GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG GCATCCCCCG AAGTAAAGAA GCCAAAGCTG
    GAAGAGAAAA TGGAAGTGAA ACCAAAATCA GCAGCAGCAG TGGCCACACA GCCAGATGTG
    CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA GAAACTTCCA AACCAAGCAC TCAGAAAAAA
    GAGGAAAAAG CAAAAATAGC AGAAGTGTCT AAACCAAGCA CTCAGAAAAA AGAGGAAAAA
    CTGGCAGCAG CAGCAGACAC ATCCAAACCA AGCACTCAGA AAAAAGAGGT AAAAGCCACA
    GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA CAAAGCACTG AGTCCATACC ATCAGAAGCA
    ATAGACGAGT TGTTGGGAAC ACTTGAAGGA CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA
    TTTCAGGGTC CAGAAGTTAC TGAGAACATT ACAGCAACAT ACTTAGAAGA AACTGGAAAA
    AGAGATAGTA CGATCCCTCC AGAATACAGA AAATTATTAG ATGGAAAGGA TCATGGGAAA
    GATGTGTGGC CTTCAGCACC TGAACCAGAA CCGTCTATGA CCGACACAGA GCTTGTAGAT
    GTGTTTTCCA AAGACTTTGA GCTTTCATGT TCACCTACTG TTCAGACCAC GCCAGCCATT
    AAACCCAAAA GGAACACTGA AGATCTCCAC GAGTGCTATT GCTCAAAGTC AGTGAGCCAA
    CTTTCATCAT CTGAAACTCC TCAAGGTAAC AAAACGAAAC CTGAAATTGC GGCATCATCT
    GCATCCACTG TAAAAGCAGC AGCGCCCTCC GGTGGTATGG ACAGTGCATT AGATGAATTA
    TTGGGAACAC TGGAAGGTCC TGATCCAACA GCCCCTGAAT CACCTGTGTA CACTGGGCCA
    GAGGTGACGG AAACAGAGAC AGCGGTATAC AGAGAGGGAC TTGGGATAAG AGACAGCAGC
    ATCCCTCCAG CATACAGGCA CTTACTAGAT GATTCTGCTG GAGGAGCACT ATTAACTGAT
    GCACCCCCAC CACCGCCAGA GGAAAAGCCT CTGACCGATG ATCAGCTGAT TGATAAGTTT
    TCTATGGACT TTGATTGTTC TGAATCTCCT GCAACACAAC AGGCTCATCC TGTCGAACCT
    AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA AAAGAAGTAG TTGCTGCCTC CTCTGCTACT
    GTTAAAGCTG CACCCACTCC TTCTAAAGCA CCAGCACCTC CAGCTGATCC ACTCGATAGC
    CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA GAACCGAGTG ACAAGAAGCC TCCAGTGGAT
    AAAGTAAAGG AGGGAACAGG CAAAGAGAAG AAAGAAAAGC TTGGAGAAGA TGAAAAAACA
    ATACATCCTG ACCGCCGGCT AAAGGAAGTG AAGGATAAAG ACGGAAAGCC CTTGCTGCCA
    AAACTTGAAC AAAGCCCTAA GCCAATGAGT GAGGCCGAGC TGCTGGATGC TTTGACTGAA
    GGCTTTGTGA CTTCCCCTGC CACTCCTGAT GCCCCACTGC AGGGCTCTTT GAAAAAAAAA
    GGTTCTGGAT CAGAAGATGT TATATCTTGT TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT
    CCACAGGACG CTGCTTTAGA TATACAGATT CCCGACGATG CACTAGATTT ACTTTCTGGC
    TCCCTGGGAC AAAGAGAGGT GGATCCTAAT GAAAACAAAC CGGTGGTGGA TGTAGTTAAG
    GAAAAAGCTA AAGCTGAGCA TATAGACAAA CTGGGGGACA GAGATGATAC AATCCCACCG
    GAATACAGAA AGCTGCTGGA TGGTAAAGAT GATAAGGGAC AACCTGCAAA ACCACCAGCG
    AAAGCGGAGG AGAAACCAAA GAAGCCTTTA AGTGACGATG CAGCGATCGA TGCTCTTTCA
    AGCGGATTTG CATCATGTGA CACAAAATCT ACTGAACAAC CAAAGCAGCC TTCAACGGAC
    AAGACTGAGA AACAATGTGC TAGCGCTCCA GTGTCGCAGC CTTCAGGAAA GGCACCTGAT
    GCTGCAAAGC AGGCAAACTC AGCGATACCA AGTGTGCCAA AGGCTGGCAA AAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47525
    Clone ID Related Accession (Same CDS sequence) XM_031895407.1
    Accession Version XM_031895407.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
    CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGGCTAAACA AACACCCAAG
    ACTGAACCAG CCAAATCTAC AAAGCCAACA AAGCCAAAAG AAGCAACAAC AGCATCTTCA
    GCAGCCCTGG CGGCTGCATC CAAACCAACA GCAGCACCTT CCTCAGCCCC AGTAACAGCA
    ACCAAACCAA CAAAAACAAA GCCAAGTGCA GCAAAATCGC CTCCCAAACA AGATATAAAG
    CCTATCCCTG GGAAGCAGCA AAAAACAGAC AGCAAGAGTA AAGATGAAAA GAAAAGTGGT
    GATAAAGTTT CTCCAGCTGT GCTACCTAAG ATGACACCTA GTTCTGCTGG TAAAGTATCT
    GCAGTTGCCA CTGGAGCTCT AGCAGCTGCT GGTGCCGCCG CTGCAGTCAC TGCTGTGCAG
    TCAACAAAGC CGAAAGATGA GAGTAAAGAG GAAAAGAAAG CCATTACTGG GGATAAGGTT
    TCTTCTGGGC CACCGGCCAA GACAGACGCA GATGCAGCCT TGGACGAACT ACTTGGAACG
    TTAGAGGGCC CAGCAGATAT TCCAGAGTCT CCACAATTTA CTGGACCAGA AATCCAGGAC
    ACAGTTGTTA CATCTGAGTA TTTGGAGGAG CTAGGAAAAC GGGATTTCAC CATCCCACCT
    GAATACAGGC ACCTGCTGGA TGGTAAAGGT GAAAAGATGG CACCCCCTAC CCCACCCGAG
    GAACCCGAAG AGCACATGGA TGATGATGAA TTAGTTGCAG CCCTGTCTTC TGGTTTTGAG
    AGCAGTCAGG CATCCCCCGA AGTAAAGAAG CCAAAGCTGG AAGAGAAAAT GGAAGTGAAA
    CCAAAATCAG CAGCAGCAGT GGCCACACAG CCAGATGTGC AGAAAAAAGA AGTAAAAGTC
    GCAGCAGCAG AAACTTCCAA ACCAAGCACT CAGAAAAAAG AGGAAAAAGC AAAAATAGCA
    GAAGTGTCTA AACCAAGCAC TCAGAAAAAA GAGGAAAAAC TGGCAGCAGC AGCAGACACA
    TCCAAACCAA GCACTCAGAA AAAAGAGGTA AAAGCCACAG CAAAAGCCAC AGCTGCCGAA
    GCTTCCAAAC AAAGCACTGA GTCCATACCA TCAGAAGCAA TAGACGAGTT GTTGGGAACA
    CTTGAAGGAC CCCCAGAAAA TGTTCCAGGG TCTCCAGTAT TTCAGGGTCC AGAAGTTACT
    GAGAACATTA CAGCAACATA CTTAGAAGAA ACTGGAAAAA GAGATAGTAC GATCCCTCCA
    GAATACAGAA AATTATTAGA TGGAAAGGAT CATGGGAAAG ATGTGTGGCC TTCAGCACCT
    GAACCAGAAC CGTCTATGAC CGACACAGAG CTTGTAGATG TGTTTTCCAA AGACTTTGAG
    CTTTCATGTT CACCTACTGT TCAGACCACG CCAGCCATTA AACCCAAAAG GAACACTGAA
    GATCTCCACG AGTGCTATTG CTCAAAGTCA GTGAGCCAAC TTTCATCATC TGAAACTCCT
    CAAGGTAACA AAACGAAACC TGAAATTGCG GCATCATCTG CATCCACTGT AAAAGCAGCA
    GCGCCCTCCG GTGGTATGGA CAGTGCATTA GATGAATTAT TGGGAACACT GGAAGGTCCT
    GATCCAACAG CCCCTGAATC ACCTGTGTAC ACTGGGCCAG AGGTGACGGA AACAGAGACA
    GCGGTATACA GAGAGGGACT TGGGATAAGA GACAGCAGCA TCCCTCCAGC ATACAGGCAC
    TTACTAGATG ATTCTGCTGG AGGAGCACTA TTAACTGATG CACCCCCACC ACCGCCAGAG
    GAAAAGCCTC TGACCGATGA TCAGCTGATT GATAAGTTTT CTATGGACTT TGATTGTTCT
    GAATCTCCTG CAACACAACA GGCTCATCCT GTCGAACCTA AAGTTTCCTC GCAAGAGAAA
    AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC TCTGCTACTG TTAAAGCTGC ACCCACTCCT
    TCTAAAGCAC CAGCACCTCC AGCTGATCCA CTCGATAGCC TGTCTGGTAC CTTAGGTGTT
    CGAAAGGAAG AACCGAGTGA CAAGAAGCCT CCAGTGGATA AAGTAAAGGA GGGAACAGGC
    AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT GAAAAAACAA TACATCCTGA CCGCCGGCTA
    AAGGAAGTGA AGGATAAAGA CGGAAAGCCC TTGCTGCCAA AACTTGAACA AAGCCCTAAG
    CCAATGAGTG AGGCCGAGCT GCTGGATGCT TTGACTGAAG GCTTTGTGAC TTCCCCTGCC
    ACTCCTGATG CCCCACTGCA GGGCTCTTTG AAAAAAAAAG GTTCTGGATC AGAAGATGTT
    ATATCTTGTT CTAAAGCTTC CGCCGTCAAG TCTGGAGCTC CACAGGACGC TGCTTTAGAT
    ATACAGATTC CCGACGATGC ACTAGATTTA CTTTCTGGCT CCCTGGGACA AAGAGAGGTG
    GATCCTAATG AAAACAAACC GGTGGTGGAT GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT
    ATAGACAAAC TGGGGGACAG AGATGATACA ATCCCACCGG AATACAGAAA GCTGCTGGAT
    GGTAAAGATG ATAAGGGACA ACCTGCAAAA CCACCAGCGA AAGCGGAGGA GAAACCAAAG
    AAGCCTTTAA GTGACGATGC AGCGATCGAT GCTCTTTCAA GCGGATTTGC ATCATGTGAC
    ACAAAATCTA CTGAACAACC AAAGCAGCCT TCAACGGACA AGACTGAGAA ACAATGTGCT
    AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG GCACCTGATG CTGCAAAGCA GGCAAACTCA
    GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751267.1
    CDS205..3120
    Translation

    Target ORF information:

    RefSeq Version XM_031895407.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895407.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
    CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGGCTAAACA AACACCCAAG
    ACTGAACCAG CCAAATCTAC AAAGCCAACA AAGCCAAAAG AAGCAACAAC AGCATCTTCA
    GCAGCCCTGG CGGCTGCATC CAAACCAACA GCAGCACCTT CCTCAGCCCC AGTAACAGCA
    ACCAAACCAA CAAAAACAAA GCCAAGTGCA GCAAAATCGC CTCCCAAACA AGATATAAAG
    CCTATCCCTG GGAAGCAGCA AAAAACAGAC AGCAAGAGTA AAGATGAAAA GAAAAGTGGT
    GATAAAGTTT CTCCAGCTGT GCTACCTAAG ATGACACCTA GTTCTGCTGG TAAAGTATCT
    GCAGTTGCCA CTGGAGCTCT AGCAGCTGCT GGTGCCGCCG CTGCAGTCAC TGCTGTGCAG
    TCAACAAAGC CGAAAGATGA GAGTAAAGAG GAAAAGAAAG CCATTACTGG GGATAAGGTT
    TCTTCTGGGC CACCGGCCAA GACAGACGCA GATGCAGCCT TGGACGAACT ACTTGGAACG
    TTAGAGGGCC CAGCAGATAT TCCAGAGTCT CCACAATTTA CTGGACCAGA AATCCAGGAC
    ACAGTTGTTA CATCTGAGTA TTTGGAGGAG CTAGGAAAAC GGGATTTCAC CATCCCACCT
    GAATACAGGC ACCTGCTGGA TGGTAAAGGT GAAAAGATGG CACCCCCTAC CCCACCCGAG
    GAACCCGAAG AGCACATGGA TGATGATGAA TTAGTTGCAG CCCTGTCTTC TGGTTTTGAG
    AGCAGTCAGG CATCCCCCGA AGTAAAGAAG CCAAAGCTGG AAGAGAAAAT GGAAGTGAAA
    CCAAAATCAG CAGCAGCAGT GGCCACACAG CCAGATGTGC AGAAAAAAGA AGTAAAAGTC
    GCAGCAGCAG AAACTTCCAA ACCAAGCACT CAGAAAAAAG AGGAAAAAGC AAAAATAGCA
    GAAGTGTCTA AACCAAGCAC TCAGAAAAAA GAGGAAAAAC TGGCAGCAGC AGCAGACACA
    TCCAAACCAA GCACTCAGAA AAAAGAGGTA AAAGCCACAG CAAAAGCCAC AGCTGCCGAA
    GCTTCCAAAC AAAGCACTGA GTCCATACCA TCAGAAGCAA TAGACGAGTT GTTGGGAACA
    CTTGAAGGAC CCCCAGAAAA TGTTCCAGGG TCTCCAGTAT TTCAGGGTCC AGAAGTTACT
    GAGAACATTA CAGCAACATA CTTAGAAGAA ACTGGAAAAA GAGATAGTAC GATCCCTCCA
    GAATACAGAA AATTATTAGA TGGAAAGGAT CATGGGAAAG ATGTGTGGCC TTCAGCACCT
    GAACCAGAAC CGTCTATGAC CGACACAGAG CTTGTAGATG TGTTTTCCAA AGACTTTGAG
    CTTTCATGTT CACCTACTGT TCAGACCACG CCAGCCATTA AACCCAAAAG GAACACTGAA
    GATCTCCACG AGTGCTATTG CTCAAAGTCA GTGAGCCAAC TTTCATCATC TGAAACTCCT
    CAAGGTAACA AAACGAAACC TGAAATTGCG GCATCATCTG CATCCACTGT AAAAGCAGCA
    GCGCCCTCCG GTGGTATGGA CAGTGCATTA GATGAATTAT TGGGAACACT GGAAGGTCCT
    GATCCAACAG CCCCTGAATC ACCTGTGTAC ACTGGGCCAG AGGTGACGGA AACAGAGACA
    GCGGTATACA GAGAGGGACT TGGGATAAGA GACAGCAGCA TCCCTCCAGC ATACAGGCAC
    TTACTAGATG ATTCTGCTGG AGGAGCACTA TTAACTGATG CACCCCCACC ACCGCCAGAG
    GAAAAGCCTC TGACCGATGA TCAGCTGATT GATAAGTTTT CTATGGACTT TGATTGTTCT
    GAATCTCCTG CAACACAACA GGCTCATCCT GTCGAACCTA AAGTTTCCTC GCAAGAGAAA
    AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC TCTGCTACTG TTAAAGCTGC ACCCACTCCT
    TCTAAAGCAC CAGCACCTCC AGCTGATCCA CTCGATAGCC TGTCTGGTAC CTTAGGTGTT
    CGAAAGGAAG AACCGAGTGA CAAGAAGCCT CCAGTGGATA AAGTAAAGGA GGGAACAGGC
    AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT GAAAAAACAA TACATCCTGA CCGCCGGCTA
    AAGGAAGTGA AGGATAAAGA CGGAAAGCCC TTGCTGCCAA AACTTGAACA AAGCCCTAAG
    CCAATGAGTG AGGCCGAGCT GCTGGATGCT TTGACTGAAG GCTTTGTGAC TTCCCCTGCC
    ACTCCTGATG CCCCACTGCA GGGCTCTTTG AAAAAAAAAG GTTCTGGATC AGAAGATGTT
    ATATCTTGTT CTAAAGCTTC CGCCGTCAAG TCTGGAGCTC CACAGGACGC TGCTTTAGAT
    ATACAGATTC CCGACGATGC ACTAGATTTA CTTTCTGGCT CCCTGGGACA AAGAGAGGTG
    GATCCTAATG AAAACAAACC GGTGGTGGAT GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT
    ATAGACAAAC TGGGGGACAG AGATGATACA ATCCCACCGG AATACAGAAA GCTGCTGGAT
    GGTAAAGATG ATAAGGGACA ACCTGCAAAA CCACCAGCGA AAGCGGAGGA GAAACCAAAG
    AAGCCTTTAA GTGACGATGC AGCGATCGAT GCTCTTTCAA GCGGATTTGC ATCATGTGAC
    ACAAAATCTA CTGAACAACC AAAGCAGCCT TCAACGGACA AGACTGAGAA ACAATGTGCT
    AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG GCACCTGATG CTGCAAAGCA GGCAAACTCA
    GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47526
    Clone ID Related Accession (Same CDS sequence) XM_031895410.1
    Accession Version XM_031895410.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT
    CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA
    GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG
    CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT
    CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG
    GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA
    CTAGATGATT CTGCTGGAGG AGCACTATTA ACTGATGCAC CCCCACCACC GCCAGAGGAA
    AAGCCTCTGA CCGATGATCA GCTGATTGAT AAGTTTTCTA TGGACTTTGA TTGTTCTGAA
    TCTCCTGCAA CACAACAGGC TCATCCTGTC GAACCTAAAG TTTCCTCGCA AGAGAAAAAG
    GCAGAAAAAG AAGTAGTTGC TGCCTCCTCT GCTACTGTTA AAGCTGCACC CACTCCTTCT
    AAAGCACCAG CACCTCCAGC TGATCCACTC GATAGCCTGT CTGGTACCTT AGGTGTTCGA
    AAGGAAGAAC CGAGTGACAA GAAGCCTCCA GTGGATAAAG TAAAGGAGGG AACAGGCAAA
    GAGAAGAAAG AAAAGCTTGG AGAAGATGAA AAAACAATAC ATCCTGACCG CCGGCTAAAG
    GAAGTGAAGG ATAAAGACGG AAAGCCCTTG CTGCCAAAAC TTGAACAAAG CCCTAAGCCA
    ATGAGTGAGG CCGAGCTGCT GGATGCTTTG ACTGAAGGCT TTGTGACTTC CCCTGCCACT
    CCTGATGCCC CACTGCAGGG CTCTTTGAAA AAAAAAGGTT CTGGATCAGA AGATGTTATA
    TCTTGTTCTA AAGCTTCCGC CGTCAAGTCT GGAGCTCCAC AGGACGCTGC TTTAGATATA
    CAGATTCCCG ACGATGCACT AGATTTACTT TCTGGCTCCC TGGGACAAAG AGAGGTGGAT
    CCTAATGAAA ACAAACCGGT GGTGGATGTA GTTAAGGAAA AAGCTAAAGC TGAGCATATA
    GACAAACTGG GGGACAGAGA TGATACAATC CCACCGGAAT ACAGAAAGCT GCTGGATGGT
    AAAGATGATA AGGGACAACC TGCAAAACCA CCAGCGAAAG CGGAGGAGAA ACCAAAGAAG
    CCTTTAAGTG ACGATGCAGC GATCGATGCT CTTTCAAGCG GATTTGCATC ATGTGACACA
    AAATCTACTG AACAACCAAA GCAGCCTTCA ACGGACAAGA CTGAGAAACA ATGTGCTAGC
    GCTCCAGTGT CGCAGCCTTC AGGAAAGGCA CCTGATGCTG CAAAGCAGGC AAACTCAGCG
    ATACCAAGTG TGCCAAAGGC TGGCAAAAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751270.1
    CDS205..3117
    Translation

    Target ORF information:

    RefSeq Version XM_031895410.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895410.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT
    CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA
    GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG
    CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT
    CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG
    GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA
    CTAGATGATT CTGCTGGAGG AGCACTATTA ACTGATGCAC CCCCACCACC GCCAGAGGAA
    AAGCCTCTGA CCGATGATCA GCTGATTGAT AAGTTTTCTA TGGACTTTGA TTGTTCTGAA
    TCTCCTGCAA CACAACAGGC TCATCCTGTC GAACCTAAAG TTTCCTCGCA AGAGAAAAAG
    GCAGAAAAAG AAGTAGTTGC TGCCTCCTCT GCTACTGTTA AAGCTGCACC CACTCCTTCT
    AAAGCACCAG CACCTCCAGC TGATCCACTC GATAGCCTGT CTGGTACCTT AGGTGTTCGA
    AAGGAAGAAC CGAGTGACAA GAAGCCTCCA GTGGATAAAG TAAAGGAGGG AACAGGCAAA
    GAGAAGAAAG AAAAGCTTGG AGAAGATGAA AAAACAATAC ATCCTGACCG CCGGCTAAAG
    GAAGTGAAGG ATAAAGACGG AAAGCCCTTG CTGCCAAAAC TTGAACAAAG CCCTAAGCCA
    ATGAGTGAGG CCGAGCTGCT GGATGCTTTG ACTGAAGGCT TTGTGACTTC CCCTGCCACT
    CCTGATGCCC CACTGCAGGG CTCTTTGAAA AAAAAAGGTT CTGGATCAGA AGATGTTATA
    TCTTGTTCTA AAGCTTCCGC CGTCAAGTCT GGAGCTCCAC AGGACGCTGC TTTAGATATA
    CAGATTCCCG ACGATGCACT AGATTTACTT TCTGGCTCCC TGGGACAAAG AGAGGTGGAT
    CCTAATGAAA ACAAACCGGT GGTGGATGTA GTTAAGGAAA AAGCTAAAGC TGAGCATATA
    GACAAACTGG GGGACAGAGA TGATACAATC CCACCGGAAT ACAGAAAGCT GCTGGATGGT
    AAAGATGATA AGGGACAACC TGCAAAACCA CCAGCGAAAG CGGAGGAGAA ACCAAAGAAG
    CCTTTAAGTG ACGATGCAGC GATCGATGCT CTTTCAAGCG GATTTGCATC ATGTGACACA
    AAATCTACTG AACAACCAAA GCAGCCTTCA ACGGACAAGA CTGAGAAACA ATGTGCTAGC
    GCTCCAGTGT CGCAGCCTTC AGGAAAGGCA CCTGATGCTG CAAAGCAGGC AAACTCAGCG
    ATACCAAGTG TGCCAAAGGC TGGCAAAAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47527
    Clone ID Related Accession (Same CDS sequence) XM_031895413.1
    Accession Version XM_031895413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
    GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG
    CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCTGGCGG CTGCATCCAA ACCAACAGCA
    GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA
    AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC
    AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGATG
    ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT
    GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA
    AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT
    GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA
    CAATTTACTG GACCAGAAAT CCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA
    GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA
    AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA
    GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA
    AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA
    GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG
    AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG
    GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA
    GCCACAGCAA AAGCCACAGC TGCCGAAGCT TCCAAACAAA GCACTGAGTC CATACCATCA
    GAAGCAATAG ACGAGTTGTT GGGAACACTT GAAGGACCCC CAGAAAATGT TCCAGGGTCT
    CCAGTATTTC AGGGTCCAGA AGTTACTGAG AACATTACAG CAACATACTT AGAAGAAACT
    GGAAAAAGAG ATAGTACGAT CCCTCCAGAA TACAGAAAAT TATTAGATGG AAAGGATCAT
    GGGAAAGATG TGTGGCCTTC AGCACCTGAA CCAGAACCGT CTATGACCGA CACAGAGCTT
    GTAGATGTGT TTTCCAAAGA CTTTGAGCTT TCATGTTCAC CTACTGTTCA GACCACGCCA
    GCCATTAAAC CCAAAAGGAA CACTGAAGAT CTCCACGAGT GCTATTGCTC AAAGTCAGTG
    AGCCAACTTT CATCATCTGA AACTCCTCAA GGTAACAAAA CGAAACCTGA AATTGCGGCA
    TCATCTGCAT CCACTGTAAA AGCAGCAGCG CCCTCCGGTG GTATGGACAG TGCATTAGAT
    GAATTATTGG GAACACTGGA AGGTCCTGAT CCAACAGCCC CTGAATCACC TGTGTACACT
    GGGCCAGAGG TGACGGAAAC AGAGACAGCG GTATACAGAG AGGGACTTGG GATAAGAGAC
    AGCAGCATCC CTCCAGCATA CAGGCACTTA CTAGATGATT CTGCTGGAGG AGCACTATTA
    ACTGATGCAC CCCCACCACC GCCAGAGGAA AAGCCTCTGA CCGATGATCA GCTGATTGAT
    AAGTTTTCTA TGGACTTTGA TTGTTCTGAA TCTCCTGCAA CACAACAGGC TCATCCTGTC
    GAACCTAAAG TTTCCTCGCA AGAGAAAAAG GCAGAAAAAG AAGTAGTTGC TGCCTCCTCT
    GCTACTGTTA AAGCTGCACC CACTCCTTCT AAAGCACCAG CACCTCCAGC TGATCCACTC
    GATAGCCTGT CTGGTACCTT AGGTGTTCGA AAGGAAGAAC CGAGTGACAA GAAGCCTCCA
    GTGGATAAAG TAAAGGAGGG AACAGGCAAA GAGAAGAAAG AAAAGCTTGG AGAAGATGAA
    AAAACAATAC ATCCTGACCG CCGGCTAAAG GAAGTGAAGG ATAAAGACGG AAAGCCCTTG
    CTGCCAAAAC TTGAACAAAG CCCTAAGCCA ATGAGTGAGG CCGAGCTGCT GGATGCTTTG
    ACTGAAGGCT TTGTGACTTC CCCTGCCACT CCTGATGCCC CACTGCAGGG CTCTTTGAAA
    AAAAAAGGTT CTGGATCAGA AGATGTTATA TCTTGTTCTA AAGCTTCCGC CGTCAAGTCT
    GGAGCTCCAC AGGACGCTGC TTTAGATATA CAGATTCCCG ACGATGCACT AGATTTACTT
    TCTGGCTCCC TGGGACAAAG AGAGGTGGAT CCTAATGAAA ACAAACCGGT GGTGGATGTA
    GTTAAGGAAA AAGCTAAAGC TGAGCATATA GACAAACTGG GGGACAGAGA TGATACAATC
    CCACCGGAAT ACAGAAAGCT GCTGGATGGT AAAGATGATA AGGGACAACC TGCAAAACCA
    CCAGCGAAAG CGGAGGAGAA ACCAAAGAAG CCTTTAAGTG ACGATGCAGC GATCGATGCT
    CTTTCAAGCG GATTTGCATC ATGTGACACA AAATCTACTG AACAACCAAA GCAGCCTTCA
    ACGGACAAGA CTGAGAAACA ATGTGCTAGC GCTCCAGTGT CGCAGCCTTC AGGAAAGGCA
    CCTGATGCTG CAAAGCAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751273.1
    CDS145..3027
    Translation

    Target ORF information:

    RefSeq Version XM_031895413.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
    GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG
    CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCTGGCGG CTGCATCCAA ACCAACAGCA
    GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA
    AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC
    AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGATG
    ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT
    GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA
    AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT
    GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA
    CAATTTACTG GACCAGAAAT CCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA
    GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA
    AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA
    GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA
    AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA
    GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG
    AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG
    GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA
    GCCACAGCAA AAGCCACAGC TGCCGAAGCT TCCAAACAAA GCACTGAGTC CATACCATCA
    GAAGCAATAG ACGAGTTGTT GGGAACACTT GAAGGACCCC CAGAAAATGT TCCAGGGTCT
    CCAGTATTTC AGGGTCCAGA AGTTACTGAG AACATTACAG CAACATACTT AGAAGAAACT
    GGAAAAAGAG ATAGTACGAT CCCTCCAGAA TACAGAAAAT TATTAGATGG AAAGGATCAT
    GGGAAAGATG TGTGGCCTTC AGCACCTGAA CCAGAACCGT CTATGACCGA CACAGAGCTT
    GTAGATGTGT TTTCCAAAGA CTTTGAGCTT TCATGTTCAC CTACTGTTCA GACCACGCCA
    GCCATTAAAC CCAAAAGGAA CACTGAAGAT CTCCACGAGT GCTATTGCTC AAAGTCAGTG
    AGCCAACTTT CATCATCTGA AACTCCTCAA GGTAACAAAA CGAAACCTGA AATTGCGGCA
    TCATCTGCAT CCACTGTAAA AGCAGCAGCG CCCTCCGGTG GTATGGACAG TGCATTAGAT
    GAATTATTGG GAACACTGGA AGGTCCTGAT CCAACAGCCC CTGAATCACC TGTGTACACT
    GGGCCAGAGG TGACGGAAAC AGAGACAGCG GTATACAGAG AGGGACTTGG GATAAGAGAC
    AGCAGCATCC CTCCAGCATA CAGGCACTTA CTAGATGATT CTGCTGGAGG AGCACTATTA
    ACTGATGCAC CCCCACCACC GCCAGAGGAA AAGCCTCTGA CCGATGATCA GCTGATTGAT
    AAGTTTTCTA TGGACTTTGA TTGTTCTGAA TCTCCTGCAA CACAACAGGC TCATCCTGTC
    GAACCTAAAG TTTCCTCGCA AGAGAAAAAG GCAGAAAAAG AAGTAGTTGC TGCCTCCTCT
    GCTACTGTTA AAGCTGCACC CACTCCTTCT AAAGCACCAG CACCTCCAGC TGATCCACTC
    GATAGCCTGT CTGGTACCTT AGGTGTTCGA AAGGAAGAAC CGAGTGACAA GAAGCCTCCA
    GTGGATAAAG TAAAGGAGGG AACAGGCAAA GAGAAGAAAG AAAAGCTTGG AGAAGATGAA
    AAAACAATAC ATCCTGACCG CCGGCTAAAG GAAGTGAAGG ATAAAGACGG AAAGCCCTTG
    CTGCCAAAAC TTGAACAAAG CCCTAAGCCA ATGAGTGAGG CCGAGCTGCT GGATGCTTTG
    ACTGAAGGCT TTGTGACTTC CCCTGCCACT CCTGATGCCC CACTGCAGGG CTCTTTGAAA
    AAAAAAGGTT CTGGATCAGA AGATGTTATA TCTTGTTCTA AAGCTTCCGC CGTCAAGTCT
    GGAGCTCCAC AGGACGCTGC TTTAGATATA CAGATTCCCG ACGATGCACT AGATTTACTT
    TCTGGCTCCC TGGGACAAAG AGAGGTGGAT CCTAATGAAA ACAAACCGGT GGTGGATGTA
    GTTAAGGAAA AAGCTAAAGC TGAGCATATA GACAAACTGG GGGACAGAGA TGATACAATC
    CCACCGGAAT ACAGAAAGCT GCTGGATGGT AAAGATGATA AGGGACAACC TGCAAAACCA
    CCAGCGAAAG CGGAGGAGAA ACCAAAGAAG CCTTTAAGTG ACGATGCAGC GATCGATGCT
    CTTTCAAGCG GATTTGCATC ATGTGACACA AAATCTACTG AACAACCAAA GCAGCCTTCA
    ACGGACAAGA CTGAGAAACA ATGTGCTAGC GCTCCAGTGT CGCAGCCTTC AGGAAAGGCA
    CCTGATGCTG CAAAGCAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47528
    Clone ID Related Accession (Same CDS sequence) XM_031895421.1
    Accession Version XM_031895421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2835bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG
    CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA
    GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG
    AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG
    ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT
    CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT
    TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT
    GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT
    GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA
    GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG
    GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT
    CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA
    AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG
    TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT
    GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC
    TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT
    GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC
    GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT
    CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC
    TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGA TTCTGCTGGA
    GGAGCACTAT TAACTGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT
    CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG
    GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT
    GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA
    GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC
    AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT
    GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC
    GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG
    CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG
    GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC
    GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA
    CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG
    GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA
    GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA
    CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA
    GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA
    AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT
    TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG
    GCTGGCAAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751281.1
    CDS205..3039
    Translation

    Target ORF information:

    RefSeq Version XM_031895421.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG
    CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA
    GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG
    AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG
    ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT
    CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT
    TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT
    GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT
    GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA
    GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG
    GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT
    CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA
    AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG
    TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT
    GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC
    TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT
    GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC
    GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT
    CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC
    TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGA TTCTGCTGGA
    GGAGCACTAT TAACTGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT
    CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG
    GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT
    GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA
    GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC
    AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT
    GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC
    GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG
    CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG
    GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC
    GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA
    CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG
    GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA
    GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA
    CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA
    GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA
    AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT
    TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG
    GCTGGCAAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47529
    Clone ID Related Accession (Same CDS sequence) XM_031895424.1
    Accession Version XM_031895424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC
    AAATCTACAA AGCCAACAAA GAGTAAAGAT GAAAAGAAAA GTGGTGATAA AGTTTCTCCA
    GCTGTGCTAC CTAAGATGAC ACCTAGTTCT GCTGGTAAAG TATCTGCAGT TGCCACTGGA
    GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA GTCACTGCTG TGCAGTCAAC AAAGCCGAAA
    GATGAGAGTA AAGAGGAAAA GAAAGCCATT ACTGGGGATA AGGTTTCTTC TGGGCCACCG
    GCCAAGACAG ACGCAGATGC AGCCTTGGAC GAACTACTTG GAACGTTAGA GGGCCCAGCA
    GATATTCCAG AGTCTCCACA ATTTACTGGA CCAGAAATCC AGGACACAGT TGTTACATCT
    GAGTATTTGG AGGAGCTAGG AAAACGGGAT TTCACCATCC CACCTGAATA CAGGCACCTG
    CTGGATGGTA AAGGTGAAAA GATGGCACCC CCTACCCCAC CCGAGGAACC CGAAGAGCAC
    ATGGATGATG ATGAATTAGT TGCAGCCCTG TCTTCTGGTT TTGAGAGCAG TCAGGCATCC
    CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG AAAATGGAAG TGAAACCAAA ATCAGCAGCA
    GCAGTGGCCA CACAGCCAGA TGTGCAGAAA AAAGAAGTAA AAGTCGCAGC AGCAGAAACT
    TCCAAACCAA GCACTCAGAA AAAAGAGGAA AAAGCAAAAA TAGCAGAAGT GTCTAAACCA
    AGCACTCAGA AAAAAGAGGA AAAACTGGCA GCAGCAGCAG ACACATCCAA ACCAAGCACT
    CAGAAAAAAG AGGTAAAAGC CACAGCAAAA GCCACAGCTG CCGAAGCTTC CAAACAAAGC
    ACTGAGTCCA TACCATCAGA AGCAATAGAC GAGTTGTTGG GAACACTTGA AGGACCCCCA
    GAAAATGTTC CAGGGTCTCC AGTATTTCAG GGTCCAGAAG TTACTGAGAA CATTACAGCA
    ACATACTTAG AAGAAACTGG AAAAAGAGAT AGTACGATCC CTCCAGAATA CAGAAAATTA
    TTAGATGGAA AGGATCATGG GAAAGATGTG TGGCCTTCAG CACCTGAACC AGAACCGTCT
    ATGACCGACA CAGAGCTTGT AGATGTGTTT TCCAAAGACT TTGAGCTTTC ATGTTCACCT
    ACTGTTCAGA CCACGCCAGC CATTAAACCC AAAAGGAACA CTGAAGATCT CCACGAGTGC
    TATTGCTCAA AGTCAGTGAG CCAACTTTCA TCATCTGAAA CTCCTCAAGG TAACAAAACG
    AAACCTGAAA TTGCGGCATC ATCTGCATCC ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT
    ATGGACAGTG CATTAGATGA ATTATTGGGA ACACTGGAAG GTCCTGATCC AACAGCCCCT
    GAATCACCTG TGTACACTGG GCCAGAGGTG ACGGAAACAG AGACAGCGGT ATACAGAGAG
    GGACTTGGGA TAAGAGACAG CAGCATCCCT CCAGCATACA GGCACTTACT AGATGATTCT
    GCTGGAGGAG CACTATTAAC TGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC
    GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA
    CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA
    GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA
    CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG
    AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA
    AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT
    AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC
    GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA
    CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA
    GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC
    GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC
    AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG
    GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG
    GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC
    GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA
    CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG
    CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG
    CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751284.1
    CDS229..3009
    Translation

    Target ORF information:

    RefSeq Version XM_031895424.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC
    AAATCTACAA AGCCAACAAA GAGTAAAGAT GAAAAGAAAA GTGGTGATAA AGTTTCTCCA
    GCTGTGCTAC CTAAGATGAC ACCTAGTTCT GCTGGTAAAG TATCTGCAGT TGCCACTGGA
    GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA GTCACTGCTG TGCAGTCAAC AAAGCCGAAA
    GATGAGAGTA AAGAGGAAAA GAAAGCCATT ACTGGGGATA AGGTTTCTTC TGGGCCACCG
    GCCAAGACAG ACGCAGATGC AGCCTTGGAC GAACTACTTG GAACGTTAGA GGGCCCAGCA
    GATATTCCAG AGTCTCCACA ATTTACTGGA CCAGAAATCC AGGACACAGT TGTTACATCT
    GAGTATTTGG AGGAGCTAGG AAAACGGGAT TTCACCATCC CACCTGAATA CAGGCACCTG
    CTGGATGGTA AAGGTGAAAA GATGGCACCC CCTACCCCAC CCGAGGAACC CGAAGAGCAC
    ATGGATGATG ATGAATTAGT TGCAGCCCTG TCTTCTGGTT TTGAGAGCAG TCAGGCATCC
    CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG AAAATGGAAG TGAAACCAAA ATCAGCAGCA
    GCAGTGGCCA CACAGCCAGA TGTGCAGAAA AAAGAAGTAA AAGTCGCAGC AGCAGAAACT
    TCCAAACCAA GCACTCAGAA AAAAGAGGAA AAAGCAAAAA TAGCAGAAGT GTCTAAACCA
    AGCACTCAGA AAAAAGAGGA AAAACTGGCA GCAGCAGCAG ACACATCCAA ACCAAGCACT
    CAGAAAAAAG AGGTAAAAGC CACAGCAAAA GCCACAGCTG CCGAAGCTTC CAAACAAAGC
    ACTGAGTCCA TACCATCAGA AGCAATAGAC GAGTTGTTGG GAACACTTGA AGGACCCCCA
    GAAAATGTTC CAGGGTCTCC AGTATTTCAG GGTCCAGAAG TTACTGAGAA CATTACAGCA
    ACATACTTAG AAGAAACTGG AAAAAGAGAT AGTACGATCC CTCCAGAATA CAGAAAATTA
    TTAGATGGAA AGGATCATGG GAAAGATGTG TGGCCTTCAG CACCTGAACC AGAACCGTCT
    ATGACCGACA CAGAGCTTGT AGATGTGTTT TCCAAAGACT TTGAGCTTTC ATGTTCACCT
    ACTGTTCAGA CCACGCCAGC CATTAAACCC AAAAGGAACA CTGAAGATCT CCACGAGTGC
    TATTGCTCAA AGTCAGTGAG CCAACTTTCA TCATCTGAAA CTCCTCAAGG TAACAAAACG
    AAACCTGAAA TTGCGGCATC ATCTGCATCC ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT
    ATGGACAGTG CATTAGATGA ATTATTGGGA ACACTGGAAG GTCCTGATCC AACAGCCCCT
    GAATCACCTG TGTACACTGG GCCAGAGGTG ACGGAAACAG AGACAGCGGT ATACAGAGAG
    GGACTTGGGA TAAGAGACAG CAGCATCCCT CCAGCATACA GGCACTTACT AGATGATTCT
    GCTGGAGGAG CACTATTAAC TGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC
    GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA
    CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA
    GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA
    CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG
    AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA
    AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT
    AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC
    GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA
    CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA
    GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC
    GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC
    AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG
    GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG
    GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC
    GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA
    CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG
    CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG
    CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31725
    Clone ID Related Accession (Same CDS sequence) XM_012956026.1 , XM_012956023.1 , XM_012956017.1 , XM_012956017.2
    Accession Version XM_012956017.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC
    AAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811471.1
    CDS205..3213
    Translation

    Target ORF information:

    RefSeq Version XM_012956017.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956017.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC
    AAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47517
    Clone ID Related Accession (Same CDS sequence) XM_031895362.1 , XM_031895367.1 , XM_031895374.1 , XM_031895373.1
    Accession Version XM_031895362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751222.1
    CDS205..3222
    Translation

    Target ORF information:

    RefSeq Version XM_031895362.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47517
    Clone ID Related Accession (Same CDS sequence) XM_031895362.1 , XM_031895367.1 , XM_031895374.1 , XM_031895373.1
    Accession Version XM_031895367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751227.1
    CDS209..3226
    Translation

    Target ORF information:

    RefSeq Version XM_031895367.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47517
    Clone ID Related Accession (Same CDS sequence) XM_031895362.1 , XM_031895367.1 , XM_031895374.1 , XM_031895373.1
    Accession Version XM_031895374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751234.1
    CDS212..3229
    Translation

    Target ORF information:

    RefSeq Version XM_031895374.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47517
    Clone ID Related Accession (Same CDS sequence) XM_031895362.1 , XM_031895367.1 , XM_031895374.1 , XM_031895373.1
    Accession Version XM_031895373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751233.1
    CDS134..3151
    Translation

    Target ORF information:

    RefSeq Version XM_031895373.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47518
    Clone ID Related Accession (Same CDS sequence) XM_031895377.1
    Accession Version XM_031895377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG GCAAACTCAG CGATACCAAG TGTGCCAAAG
    GCTGGCAAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751237.1
    CDS205..3219
    Translation

    Target ORF information:

    RefSeq Version XM_031895377.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG GCAAACTCAG CGATACCAAG TGTGCCAAAG
    GCTGGCAAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47519
    Clone ID Related Accession (Same CDS sequence) XM_031895380.1
    Accession Version XM_031895380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCAGTGCT TTTTAAACCT
    TCCTTGCACC ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751240.1
    CDS205..3219
    Translation

    Target ORF information:

    RefSeq Version XM_031895380.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT
    GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCAGTGCT TTTTAAACCT
    TCCTTGCACC ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa21874
    Clone ID Related Accession (Same CDS sequence) NM_001126837.1
    Accession Version NM_001126837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC160635.1. ##Evidence-Data-START## Transcript exon combination :: BC160635.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
    GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG
    CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCCAGCGG CTGCAACCAA ACCAACAGCA
    GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA
    AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC
    AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGGTG
    ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT
    GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA
    AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT
    GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA
    CAATTTACTG GACCAGAAAT TCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA
    GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA
    AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA
    GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA
    AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA
    GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG
    AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG
    GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA
    GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC
    GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGAGTCTCC AGTATTTCAT
    GGTCCTGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT
    ATTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG
    TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CACAGCTTGT AGATGAGTTT
    TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC
    AAAGAAACTC CTCAAGGTAA CAAAACGAAA CCTGAAATTG CAGCATCATC TGCATCCACT
    GTAAAAGCAG CAGCGCCCTC CGGTGGTATG GACAGTGCAT TAGATGAATT ATTGGGAACA
    CTGGAAGGTC CTGATCCAAC AGCCCCTGAA TCACCTGTGT ACACTGGGCC AGAGGTGACG
    GAAACAGAGA CAGCGGTATA CAGAGAGGGA CTTGGGATAA GAGACAGCAG CATCCCTCCA
    GCATACAGGC ACTTACTAGA TGGGAAAGTA GATGGAAAAG ATGCACCCCC ACCACCGCCA
    GAGGAAAAGC CTCTGACCGA TGATCAGCTG ATTGATGAGT TTTCTATGGA CTTTGATTGT
    TCTGAATCTC CTGCAACACA ACAGGCTCAT CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG
    AAAAAGGCAG AAAAAGAAGT AGTCGCTGCC TCCTCTGCTA CTGTTAAAGC TGCACCCACT
    CCTTCTAAAG CACCAGCACC TCCAGCTGAT CCACTCGATA GCCTGTCTGG TACCTTAGGT
    TTTCGAAAGG AAGAACCGAG TGACAAGAAG CCTCCAGTGG ATAAAGTAAA GGAGGGAACA
    GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA GATGAAAAAA CAATACATCC TGACCGCCGG
    CTAAAGGAAG TGAAGGATAA AGATGGAAAG CCCTTGCTGC CAAAACTTGA ACAAAGCCCT
    AAGCCAATGA GTGAGGCCGA GCTGCTGGAT GCTTTGACTG AAGGCTTTGT GACTTCCCCT
    GCCACTCCTG ATGCCCCACT GCAGGGCTCT CTGAAAAAAA AAGGTTCTGG ATCAGAAGAT
    GTTATATCTT GTTCTAAAGC TTCCGCCGTC AAGTCTGGAG CTCCACAGGA CGCTGCTTTA
    GATATACAGA TTCCCGACGA TGCACTAGAT TTACTTTCTG GCTCCCTGGG ACAAAGAGAG
    GTGGATCCTA ATGAAAACAA ACCGGTGGTG GATGTAGTTA AGGAAAAAGC TAAAGCTGAG
    CATATAGACA AACTGGGGGA CAGAGATGAT ACAATCCCAC CGGAATACAG AAAGCTGCTG
    GATGGTAAAG ATGATAAGGG ACAACCTGCA AAACCACCAG CGAAAGCGGA GGAGAAACCA
    AAGAAGCCTT TAAGTGACGA TGCAGCGATC GATGCTCTTT CAAGCGGATT TGCATCATGT
    GACACAAAAT CTACTGAACA ACCAAAGCAG CCTTCAACGG ACAAGACTGA GAAACAATGT
    GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA AAGGCACCTG ATGCTGCAAA GGCAAACTCA
    GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120309.1
    CDS32..2827
    Translation

    Target ORF information:

    RefSeq Version NM_001126837.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001126837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
    GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG
    CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCCAGCGG CTGCAACCAA ACCAACAGCA
    GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA
    AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC
    AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGGTG
    ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT
    GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA
    AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT
    GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA
    CAATTTACTG GACCAGAAAT TCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA
    GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA
    AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA
    GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA
    AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA
    GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG
    AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG
    GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA
    GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC
    GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGAGTCTCC AGTATTTCAT
    GGTCCTGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT
    ATTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG
    TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CACAGCTTGT AGATGAGTTT
    TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC
    AAAGAAACTC CTCAAGGTAA CAAAACGAAA CCTGAAATTG CAGCATCATC TGCATCCACT
    GTAAAAGCAG CAGCGCCCTC CGGTGGTATG GACAGTGCAT TAGATGAATT ATTGGGAACA
    CTGGAAGGTC CTGATCCAAC AGCCCCTGAA TCACCTGTGT ACACTGGGCC AGAGGTGACG
    GAAACAGAGA CAGCGGTATA CAGAGAGGGA CTTGGGATAA GAGACAGCAG CATCCCTCCA
    GCATACAGGC ACTTACTAGA TGGGAAAGTA GATGGAAAAG ATGCACCCCC ACCACCGCCA
    GAGGAAAAGC CTCTGACCGA TGATCAGCTG ATTGATGAGT TTTCTATGGA CTTTGATTGT
    TCTGAATCTC CTGCAACACA ACAGGCTCAT CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG
    AAAAAGGCAG AAAAAGAAGT AGTCGCTGCC TCCTCTGCTA CTGTTAAAGC TGCACCCACT
    CCTTCTAAAG CACCAGCACC TCCAGCTGAT CCACTCGATA GCCTGTCTGG TACCTTAGGT
    TTTCGAAAGG AAGAACCGAG TGACAAGAAG CCTCCAGTGG ATAAAGTAAA GGAGGGAACA
    GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA GATGAAAAAA CAATACATCC TGACCGCCGG
    CTAAAGGAAG TGAAGGATAA AGATGGAAAG CCCTTGCTGC CAAAACTTGA ACAAAGCCCT
    AAGCCAATGA GTGAGGCCGA GCTGCTGGAT GCTTTGACTG AAGGCTTTGT GACTTCCCCT
    GCCACTCCTG ATGCCCCACT GCAGGGCTCT CTGAAAAAAA AAGGTTCTGG ATCAGAAGAT
    GTTATATCTT GTTCTAAAGC TTCCGCCGTC AAGTCTGGAG CTCCACAGGA CGCTGCTTTA
    GATATACAGA TTCCCGACGA TGCACTAGAT TTACTTTCTG GCTCCCTGGG ACAAAGAGAG
    GTGGATCCTA ATGAAAACAA ACCGGTGGTG GATGTAGTTA AGGAAAAAGC TAAAGCTGAG
    CATATAGACA AACTGGGGGA CAGAGATGAT ACAATCCCAC CGGAATACAG AAAGCTGCTG
    GATGGTAAAG ATGATAAGGG ACAACCTGCA AAACCACCAG CGAAAGCGGA GGAGAAACCA
    AAGAAGCCTT TAAGTGACGA TGCAGCGATC GATGCTCTTT CAAGCGGATT TGCATCATGT
    GACACAAAAT CTACTGAACA ACCAAAGCAG CCTTCAACGG ACAAGACTGA GAAACAATGT
    GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA AAGGCACCTG ATGCTGCAAA GGCAAACTCA
    GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31725
    Clone ID Related Accession (Same CDS sequence) XM_012956026.1 , XM_012956023.1 , XM_012956017.1 , XM_012956017.2
    Accession Version XM_012956026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC
    AAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811480.1
    CDS212..3220
    Translation

    Target ORF information:

    RefSeq Version XM_012956026.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC
    AAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31725
    Clone ID Related Accession (Same CDS sequence) XM_012956026.1 , XM_012956023.1 , XM_012956017.1 , XM_012956017.2
    Accession Version XM_012956023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC
    AAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811477.1
    CDS103..3111
    Translation

    Target ORF information:

    RefSeq Version XM_012956023.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC
    AAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31725
    Clone ID Related Accession (Same CDS sequence) XM_012956026.1 , XM_012956023.1 , XM_012956017.1 , XM_012956017.2
    Accession Version XM_012956017.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC
    AAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811471.1
    CDS206..3214
    Translation

    Target ORF information:

    RefSeq Version XM_012956017.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC
    AAAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31726
    Clone ID Related Accession (Same CDS sequence) XM_012956032.1
    Accession Version XM_012956032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811486.1
    CDS206..3211
    Translation

    Target ORF information:

    RefSeq Version XM_012956032.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31727
    Clone ID Related Accession (Same CDS sequence) XM_012956040.1
    Accession Version XM_012956040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCAGTGC TTTTTAAACC TTCCTTGCAC
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811494.1
    CDS206..3211
    Translation

    Target ORF information:

    RefSeq Version XM_012956040.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG
    CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT
    GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT
    TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC
    AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT
    TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG
    GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT
    ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA
    AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC
    GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG
    CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA
    AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCAGTGC TTTTTAAACC TTCCTTGCAC
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31728
    Clone ID Related Accession (Same CDS sequence) XM_012956046.2
    Accession Version XM_012956046.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
    CCATCAGAAG GAGCAGAGAA GAAAACTGGT GCCAGTGGCC CTTCAGGGGC CCAAGCAAGT
    GGGACCAGCA AGACTCCTAG TGCTTCAGGA AAAACTATGA ACCCGGCGGA TACACAGGCT
    AAACAAACAC CCAAGACTGA ACCAGCCAAA TCTACAAAGC CAACAAAGCC AAAAGAAGCA
    ACAACAGCAT CTTCAGCAGC CCTGGCGGCT GCATCCAAAC CAACAGCAGC ACCTTCCTCA
    GCCCCAGTAA CAGCAACCAA ACCAACAAAA ACAAAGCCAA GTGCAGCAAA ATCGCCTCCC
    AAACAAGATA TAAAGCCTAT CCCTGGGAAG CAGCAAAAAA CAGACAGCAA GAGTAAAGAT
    GAAAAGAAAA GTGGTGATAA AGTTTCTCCA GCTGTGCTAC CTAAGATGAC ACCTAGTTCT
    GCTGGTAAAG TATCTGCAGT TGCCACTGGA GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA
    GTCACTGCTG TGCAGTCAAC AAAGCCGAAA GATGAGAGTA AAGAGGAAAA GAAAGCCATT
    ACTGGGGATA AGGTTTCTTC TGGGCCACCG GCCAAGACAG ACGCAGATGC AGCCTTGGAC
    GAACTACTTG GAACGTTAGA GGGCCCAGCA GATATTCCAG AGTCTCCACA ATTTACTGGA
    CCAGAAATCC AGGACACAGT TGTTACATCT GAGTATTTGG AGGAGCTAGG AAAACGGGAT
    TTCACCATCC CACCTGAATA CAGGCACCTG CTGGATGGTA AAGGTGAAAA GATGGCACCC
    CCTACCCCAC CCGAGGAACC CGAAGAGCAC ATGGATGATG ATGAATTAGT TGCAGCCCTG
    TCTTCTGGTT TTGAGAGCAG TCAGGCATCC CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG
    AAAATGGAAG TGAAACCAAA ATCAGCAGCA GCAGTGGCCA CACAGCCAGA TGTGCAGAAA
    AAAGAAGTAA AAGTCGCAGC AGCAGAAACT TCCAAACCAA GCACTCAGAA AAAAGAGGAA
    AAAGCAAAAA TAGCAGAAGT GTCTAAACCA AGCACTCAGA AAAAAGAGGA AAAACTGGCA
    GCAGCAGCAG ACACATCCAA ACCAAGCACT CAGAAAAAAG AGGTAAAAGC CACAGCAAAA
    GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC
    GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGGGTCTCC AGTATTTCAG
    GGTCCAGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT
    AGTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG
    TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CAGAGCTTGT AGATGTGTTT
    TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC
    AAAAGGAACA CTGAAGATCT CCACGAGTGC TATTGCTCAA AGTCAGTGAG CCAACTTTCA
    TCATCTGAAA CTCCTCAAGG TAACAAAACG AAACCTGAAA TTGCGGCATC ATCTGCATCC
    ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT ATGGACAGTG CATTAGATGA ATTATTGGGA
    ACACTGGAAG GTCCTGATCC AACAGCCCCT GAATCACCTG TGTACACTGG GCCAGAGGTG
    ACGGAAACAG AGACAGCGGT ATACAGAGAG GGACTTGGGA TAAGAGACAG CAGCATCCCT
    CCAGCATACA GGCACTTACT AGATGGGAAA GTAGATGGAA AAGATGCACC CCCACCACCG
    CCAGAGGAAA AGCCTCTGAC CGATGATCAG CTGATTGATA AGTTTTCTAT GGACTTTGAT
    TGTTCTGAAT CTCCTGCAAC ACAACAGGCT CATCCTGTCG AACCTAAAGT TTCCTCGCAA
    GAGAAAAAGG CAGAAAAAGA AGTAGTTGCT GCCTCCTCTG CTACTGTTAA AGCTGCACCC
    ACTCCTTCTA AAGCACCAGC ACCTCCAGCT GATCCACTCG ATAGCCTGTC TGGTACCTTA
    GGTGTTCGAA AGGAAGAACC GAGTGACAAG AAGCCTCCAG TGGATAAAGT AAAGGAGGGA
    ACAGGCAAAG AGAAGAAAGA AAAGCTTGGA GAAGATGAAA AAACAATACA TCCTGACCGC
    CGGCTAAAGG AAGTGAAGGA TAAAGACGGA AAGCCCTTGC TGCCAAAACT TGAACAAAGC
    CCTAAGCCAA TGAGTGAGGC CGAGCTGCTG GATGCTTTGA CTGAAGGCTT TGTGACTTCC
    CCTGCCACTC CTGATGCCCC ACTGCAGGGC TCTTTGAAAA AAAAAGGTTC TGGATCAGAA
    GATGTTATAT CTTGTTCTAA AGCTTCCGCC GTCAAGTCTG GAGCTCCACA GGACGCTGCT
    TTAGATATAC AGATTCCCGA CGATGCACTA GATTTACTTT CTGGCTCCCT GGGACAAAGA
    GAGGTGGATC CTAATGAAAA CAAACCGGTG GTGGATGTAG TTAAGGAAAA AGCTAAAGCT
    GAGCATATAG ACAAACTGGG GGACAGAGAT GATACAATCC CACCGGAATA CAGAAAGCTG
    CTGGATGGTA AAGATGATAA GGGACAACCT GCAAAACCAC CAGCGAAAGC GGAGGAGAAA
    CCAAAGAAGC CTTTAAGTGA CGATGCAGCG ATCGATGCTC TTTCAAGCGG ATTTGCATCA
    TGTGACACAA AATCTACTGA ACAACCAAAG CAGCCTTCAA CGGACAAGAC TGAGAAACAA
    TGTGCTAGCG CTCCAGTGTC GCAGCCTTCA GGAAAGGCAC CTGATGCTGC AAAGCAGGCA
    AACTCAGCGA TACCAAGTGT GCCAAAGGCT GGCAAAAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811500.1
    CDS210..3191
    Translation

    Target ORF information:

    RefSeq Version XM_012956046.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956046.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
    CCATCAGAAG GAGCAGAGAA GAAAACTGGT GCCAGTGGCC CTTCAGGGGC CCAAGCAAGT
    GGGACCAGCA AGACTCCTAG TGCTTCAGGA AAAACTATGA ACCCGGCGGA TACACAGGCT
    AAACAAACAC CCAAGACTGA ACCAGCCAAA TCTACAAAGC CAACAAAGCC AAAAGAAGCA
    ACAACAGCAT CTTCAGCAGC CCTGGCGGCT GCATCCAAAC CAACAGCAGC ACCTTCCTCA
    GCCCCAGTAA CAGCAACCAA ACCAACAAAA ACAAAGCCAA GTGCAGCAAA ATCGCCTCCC
    AAACAAGATA TAAAGCCTAT CCCTGGGAAG CAGCAAAAAA CAGACAGCAA GAGTAAAGAT
    GAAAAGAAAA GTGGTGATAA AGTTTCTCCA GCTGTGCTAC CTAAGATGAC ACCTAGTTCT
    GCTGGTAAAG TATCTGCAGT TGCCACTGGA GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA
    GTCACTGCTG TGCAGTCAAC AAAGCCGAAA GATGAGAGTA AAGAGGAAAA GAAAGCCATT
    ACTGGGGATA AGGTTTCTTC TGGGCCACCG GCCAAGACAG ACGCAGATGC AGCCTTGGAC
    GAACTACTTG GAACGTTAGA GGGCCCAGCA GATATTCCAG AGTCTCCACA ATTTACTGGA
    CCAGAAATCC AGGACACAGT TGTTACATCT GAGTATTTGG AGGAGCTAGG AAAACGGGAT
    TTCACCATCC CACCTGAATA CAGGCACCTG CTGGATGGTA AAGGTGAAAA GATGGCACCC
    CCTACCCCAC CCGAGGAACC CGAAGAGCAC ATGGATGATG ATGAATTAGT TGCAGCCCTG
    TCTTCTGGTT TTGAGAGCAG TCAGGCATCC CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG
    AAAATGGAAG TGAAACCAAA ATCAGCAGCA GCAGTGGCCA CACAGCCAGA TGTGCAGAAA
    AAAGAAGTAA AAGTCGCAGC AGCAGAAACT TCCAAACCAA GCACTCAGAA AAAAGAGGAA
    AAAGCAAAAA TAGCAGAAGT GTCTAAACCA AGCACTCAGA AAAAAGAGGA AAAACTGGCA
    GCAGCAGCAG ACACATCCAA ACCAAGCACT CAGAAAAAAG AGGTAAAAGC CACAGCAAAA
    GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC
    GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGGGTCTCC AGTATTTCAG
    GGTCCAGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT
    AGTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG
    TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CAGAGCTTGT AGATGTGTTT
    TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC
    AAAAGGAACA CTGAAGATCT CCACGAGTGC TATTGCTCAA AGTCAGTGAG CCAACTTTCA
    TCATCTGAAA CTCCTCAAGG TAACAAAACG AAACCTGAAA TTGCGGCATC ATCTGCATCC
    ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT ATGGACAGTG CATTAGATGA ATTATTGGGA
    ACACTGGAAG GTCCTGATCC AACAGCCCCT GAATCACCTG TGTACACTGG GCCAGAGGTG
    ACGGAAACAG AGACAGCGGT ATACAGAGAG GGACTTGGGA TAAGAGACAG CAGCATCCCT
    CCAGCATACA GGCACTTACT AGATGGGAAA GTAGATGGAA AAGATGCACC CCCACCACCG
    CCAGAGGAAA AGCCTCTGAC CGATGATCAG CTGATTGATA AGTTTTCTAT GGACTTTGAT
    TGTTCTGAAT CTCCTGCAAC ACAACAGGCT CATCCTGTCG AACCTAAAGT TTCCTCGCAA
    GAGAAAAAGG CAGAAAAAGA AGTAGTTGCT GCCTCCTCTG CTACTGTTAA AGCTGCACCC
    ACTCCTTCTA AAGCACCAGC ACCTCCAGCT GATCCACTCG ATAGCCTGTC TGGTACCTTA
    GGTGTTCGAA AGGAAGAACC GAGTGACAAG AAGCCTCCAG TGGATAAAGT AAAGGAGGGA
    ACAGGCAAAG AGAAGAAAGA AAAGCTTGGA GAAGATGAAA AAACAATACA TCCTGACCGC
    CGGCTAAAGG AAGTGAAGGA TAAAGACGGA AAGCCCTTGC TGCCAAAACT TGAACAAAGC
    CCTAAGCCAA TGAGTGAGGC CGAGCTGCTG GATGCTTTGA CTGAAGGCTT TGTGACTTCC
    CCTGCCACTC CTGATGCCCC ACTGCAGGGC TCTTTGAAAA AAAAAGGTTC TGGATCAGAA
    GATGTTATAT CTTGTTCTAA AGCTTCCGCC GTCAAGTCTG GAGCTCCACA GGACGCTGCT
    TTAGATATAC AGATTCCCGA CGATGCACTA GATTTACTTT CTGGCTCCCT GGGACAAAGA
    GAGGTGGATC CTAATGAAAA CAAACCGGTG GTGGATGTAG TTAAGGAAAA AGCTAAAGCT
    GAGCATATAG ACAAACTGGG GGACAGAGAT GATACAATCC CACCGGAATA CAGAAAGCTG
    CTGGATGGTA AAGATGATAA GGGACAACCT GCAAAACCAC CAGCGAAAGC GGAGGAGAAA
    CCAAAGAAGC CTTTAAGTGA CGATGCAGCG ATCGATGCTC TTTCAAGCGG ATTTGCATCA
    TGTGACACAA AATCTACTGA ACAACCAAAG CAGCCTTCAA CGGACAAGAC TGAGAAACAA
    TGTGCTAGCG CTCCAGTGTC GCAGCCTTCA GGAAAGGCAC CTGATGCTGC AAAGCAGGCA
    AACTCAGCGA TACCAAGTGT GCCAAAGGCT GGCAAAAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31729
    Clone ID Related Accession (Same CDS sequence) XM_012956054.1
    Accession Version XM_012956054.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA
    AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA
    CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA
    CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT
    ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA
    AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA
    CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT
    TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC
    GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC
    AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC
    GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA
    GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC
    AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT
    GGGAAAGTAG ATGGAAAAGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811508.1
    CDS206..3163
    Translation

    Target ORF information:

    RefSeq Version XM_012956054.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956054.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA
    AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA
    CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA
    CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT
    ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA
    AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA
    CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT
    TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC
    GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC
    AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC
    GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA
    GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC
    AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT
    GGGAAAGTAG ATGGAAAAGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31731
    Clone ID Related Accession (Same CDS sequence) XM_012956066.1
    Accession Version XM_012956066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC
    AAATCTACAA AGCCAACAAA GCCAAAAGAA GCAACAACAG CATCTTCAGC AGCCCTGGCG
    GCTGCATCCA AACCAACAGC AGCACCTTCC TCAGCCCCAG TAACAGCAAC CAAACCAACA
    AAAACAAAGC CAAGTGCAGC AAAATCGCCT CCCAAACAAG ATATAAAGCC TATCCCTGGG
    AAGCAGCAAA AAACAGACAG CAAGAGTAAA GATGAAAAGA AAAGTGGTGA TAAAGTTTCT
    CCAGCTGTGC TACCTAAGAT GACACCTAGT TCTGCTGGTA AAGTATCTGC AGTTGCCACT
    GGAGCTCTAG CAGCTGCTGG TGCCGCCGCT GCAGTCACTG CTGTGCAGTC AACAAAGCCG
    AAAGATGAGA GTAAAGAGGA AAAGAAAGCC ATTACTGGGG ATAAGGTTTC TTCTGGGCCA
    CCGGCCAAGA CAGACGCAGA TGCAGCCTTG GACGAACTAC TTGGAACGTT AGAGGGCCCA
    GCAGATATTC CAGAGTCTCC ACAATTTACT GGACCAGAAA TCCAGGACAC AGTTGTTACA
    TCTGAGTATT TGGAGGAGCT AGGAAAACGG GATTTCACCA TCCCACCTGA ATACAGGCAC
    CTGCTGGATG GTAAAGGTGA AAAGATGGCA CCCCCTACCC CACCCGAGGA ACCCGAAGAG
    CACATGGATG ATGATGAATT AGTTGCAGCC CTGTCTTCTG GTTTTGAGAG CAGTCAGGCA
    TCCCCCGAAG TAAAGAAGCC AAAGCTGGAA GAGAAAATGG AAGTGAAACC AAAATCAGCA
    GCAGCAGTGG CCACACAGCC AGATGTGCAG AAAAAAGAAG TAAAAGTCGC AGCAGCAGAA
    ACTTCCAAAC CAAGCACTCA GAAAAAAGAG GAAAAAGCAA AAATAGCAGA AGTGTCTAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAACTG GCAGCAGCAG CAGACACATC CAAACCAAGC
    ACTCAGAAAA AAGAGGTAAA AGCCACAGCA AAAGCCACAG CTGCCGAAGC TTCCAAACAA
    AGCACTGAGT CCATACCATC AGAAGCAATA GACGAGTTGT TGGGAACACT TGAAGGACCC
    CCAGAAAATG TTCCAGGGTC TCCAGTATTT CAGGGTCCAG AAGTTACTGA GAACATTACA
    GCAACATACT TAGAAGAAAC TGGAAAAAGA GATAGTACGA TCCCTCCAGA ATACAGAAAA
    TTATTAGATG GAAAGGATCA TGGGAAAGAT GTGTGGCCTT CAGCACCTGA ACCAGAACCG
    TCTATGACCG ACACAGAGCT TGTAGATGTG TTTTCCAAAG ACTTTGAGCT TTCATGTTCA
    CCTACTGTTC AGACCACGCC AGCCATTAAA CCCAAAAGGA ACACTGAAGA TCTCCACGAG
    TGCTATTGCT CAAAGTCAGT GAGCCAACTT TCATCATCTG AAACTCCTCA AGGTAACAAA
    ACGAAACCTG AAATTGCGGC ATCATCTGCA TCCACTGTAA AAGCAGCAGC GCCCTCCGGT
    GGTATGGACA GTGCATTAGA TGAATTATTG GGAACACTGG AAGGTCCTGA TCCAACAGCC
    CCTGAATCAC CTGTGTACAC TGGGCCAGAG GTGACGGAAA CAGAGACAGC GGTATACAGA
    GAGGGACTTG GGATAAGAGA CAGCAGCATC CCTCCAGCAT ACAGGCACTT ACTAGATGGG
    AAAGTAGATG GAAAAGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT
    CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG
    GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT
    GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA
    GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC
    AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT
    GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC
    GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG
    CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG
    GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC
    GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA
    CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG
    GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA
    GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA
    CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA
    GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA
    AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT
    TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG
    GCTGGCAAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811520.1
    CDS206..3160
    Translation

    Target ORF information:

    RefSeq Version XM_012956066.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC
    AAATCTACAA AGCCAACAAA GCCAAAAGAA GCAACAACAG CATCTTCAGC AGCCCTGGCG
    GCTGCATCCA AACCAACAGC AGCACCTTCC TCAGCCCCAG TAACAGCAAC CAAACCAACA
    AAAACAAAGC CAAGTGCAGC AAAATCGCCT CCCAAACAAG ATATAAAGCC TATCCCTGGG
    AAGCAGCAAA AAACAGACAG CAAGAGTAAA GATGAAAAGA AAAGTGGTGA TAAAGTTTCT
    CCAGCTGTGC TACCTAAGAT GACACCTAGT TCTGCTGGTA AAGTATCTGC AGTTGCCACT
    GGAGCTCTAG CAGCTGCTGG TGCCGCCGCT GCAGTCACTG CTGTGCAGTC AACAAAGCCG
    AAAGATGAGA GTAAAGAGGA AAAGAAAGCC ATTACTGGGG ATAAGGTTTC TTCTGGGCCA
    CCGGCCAAGA CAGACGCAGA TGCAGCCTTG GACGAACTAC TTGGAACGTT AGAGGGCCCA
    GCAGATATTC CAGAGTCTCC ACAATTTACT GGACCAGAAA TCCAGGACAC AGTTGTTACA
    TCTGAGTATT TGGAGGAGCT AGGAAAACGG GATTTCACCA TCCCACCTGA ATACAGGCAC
    CTGCTGGATG GTAAAGGTGA AAAGATGGCA CCCCCTACCC CACCCGAGGA ACCCGAAGAG
    CACATGGATG ATGATGAATT AGTTGCAGCC CTGTCTTCTG GTTTTGAGAG CAGTCAGGCA
    TCCCCCGAAG TAAAGAAGCC AAAGCTGGAA GAGAAAATGG AAGTGAAACC AAAATCAGCA
    GCAGCAGTGG CCACACAGCC AGATGTGCAG AAAAAAGAAG TAAAAGTCGC AGCAGCAGAA
    ACTTCCAAAC CAAGCACTCA GAAAAAAGAG GAAAAAGCAA AAATAGCAGA AGTGTCTAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAACTG GCAGCAGCAG CAGACACATC CAAACCAAGC
    ACTCAGAAAA AAGAGGTAAA AGCCACAGCA AAAGCCACAG CTGCCGAAGC TTCCAAACAA
    AGCACTGAGT CCATACCATC AGAAGCAATA GACGAGTTGT TGGGAACACT TGAAGGACCC
    CCAGAAAATG TTCCAGGGTC TCCAGTATTT CAGGGTCCAG AAGTTACTGA GAACATTACA
    GCAACATACT TAGAAGAAAC TGGAAAAAGA GATAGTACGA TCCCTCCAGA ATACAGAAAA
    TTATTAGATG GAAAGGATCA TGGGAAAGAT GTGTGGCCTT CAGCACCTGA ACCAGAACCG
    TCTATGACCG ACACAGAGCT TGTAGATGTG TTTTCCAAAG ACTTTGAGCT TTCATGTTCA
    CCTACTGTTC AGACCACGCC AGCCATTAAA CCCAAAAGGA ACACTGAAGA TCTCCACGAG
    TGCTATTGCT CAAAGTCAGT GAGCCAACTT TCATCATCTG AAACTCCTCA AGGTAACAAA
    ACGAAACCTG AAATTGCGGC ATCATCTGCA TCCACTGTAA AAGCAGCAGC GCCCTCCGGT
    GGTATGGACA GTGCATTAGA TGAATTATTG GGAACACTGG AAGGTCCTGA TCCAACAGCC
    CCTGAATCAC CTGTGTACAC TGGGCCAGAG GTGACGGAAA CAGAGACAGC GGTATACAGA
    GAGGGACTTG GGATAAGAGA CAGCAGCATC CCTCCAGCAT ACAGGCACTT ACTAGATGGG
    AAAGTAGATG GAAAAGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT
    CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG
    GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT
    GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA
    GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC
    AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT
    GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC
    GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG
    CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG
    GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC
    GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA
    CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG
    GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA
    GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA
    CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA
    GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA
    AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT
    TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG
    GCTGGCAAAA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31732
    Clone ID Related Accession (Same CDS sequence) XM_012956074.2 , XM_012956074.1
    Accession Version XM_012956074.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811528.1
    CDS206..3151
    Translation

    Target ORF information:

    RefSeq Version XM_012956074.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956074.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31733
    Clone ID Related Accession (Same CDS sequence) XM_012956081.1 , XM_012956081.2
    Accession Version XM_012956081.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811535.1
    CDS206..3148
    Translation

    Target ORF information:

    RefSeq Version XM_012956081.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31734
    Clone ID Related Accession (Same CDS sequence) XM_012956088.2
    Accession Version XM_012956088.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
    CCATCAGAAG GAGCAGAGAC TCCTAGTGCT TCAGGAAAAA CTATGAACCC GGCGGATACA
    CAGGCTAAAC AAACACCCAA GACTGAACCA GCCAAATCTA CAAAGCCAAC AAAGCCAAAA
    GAAGCAACAA CAGCATCTTC AGCAGCCCTG GCGGCTGCAT CCAAACCAAC AGCAGCACCT
    TCCTCAGCCC CAGTAACAGC AACCAAACCA ACAAAAACAA AGCCAAGTGC AGCAAAATCG
    CCTCCCAAAC AAGATATAAA GCCTATCCCT GGGAAGCAGC AAAAAACAGA CAGCAAGAGT
    AAAGATGAAA AGAAAAGTGG TGATAAAGTT TCTCCAGCTG TGCTACCTAA GATGACACCT
    AGTTCTGCTG GTAAAGTATC TGCAGTTGCC ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC
    GCTGCAGTCA CTGCTGTGCA GTCAACAAAG CCGAAAGATG AGAGTAAAGA GGAAAAGAAA
    GCCATTACTG GGGATAAGGT TTCTTCTGGG CCACCGGCCA AGACAGACGC AGATGCAGCC
    TTGGACGAAC TACTTGGAAC GTTAGAGGGC CCAGCAGATA TTCCAGAGTC TCCACAATTT
    ACTGGACCAG AAATCCAGGA CACAGTTGTT ACATCTGAGT ATTTGGAGGA GCTAGGAAAA
    CGGGATTTCA CCATCCCACC TGAATACAGG CACCTGCTGG ATGGTAAAGG TGAAAAGATG
    GCACCCCCTA CCCCACCCGA GGAACCCGAA GAGCACATGG ATGATGATGA ATTAGTTGCA
    GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG GCATCCCCCG AAGTAAAGAA GCCAAAGCTG
    GAAGAGAAAA TGGAAGTGAA ACCAAAATCA GCAGCAGCAG TGGCCACACA GCCAGATGTG
    CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA GAAACTTCCA AACCAAGCAC TCAGAAAAAA
    GAGGAAAAAG CAAAAATAGC AGAAGTGTCT AAACCAAGCA CTCAGAAAAA AGAGGAAAAA
    CTGGCAGCAG CAGCAGACAC ATCCAAACCA AGCACTCAGA AAAAAGAGGT AAAAGCCACA
    GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA CAAAGCACTG AGTCCATACC ATCAGAAGCA
    ATAGACGAGT TGTTGGGAAC ACTTGAAGGA CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA
    TTTCAGGGTC CAGAAGTTAC TGAGAACATT ACAGCAACAT ACTTAGAAGA AACTGGAAAA
    AGAGATAGTA CGATCCCTCC AGAATACAGA AAATTATTAG ATGGAAAGGA TCATGGGAAA
    GATGTGTGGC CTTCAGCACC TGAACCAGAA CCGTCTATGA CCGACACAGA GCTTGTAGAT
    GTGTTTTCCA AAGACTTTGA GCTTTCATGT TCACCTACTG TTCAGACCAC GCCAGCCATT
    AAACCCAAAA GGAACACTGA AGATCTCCAC GAGTGCTATT GCTCAAAGTC AGTGAGCCAA
    CTTTCATCAT CTGAAACTCC TCAAGGTAAC AAAACGAAAC CTGAAATTGC GGCATCATCT
    GCATCCACTG TAAAAGCAGC AGCGCCCTCC GGTGGTATGG ACAGTGCATT AGATGAATTA
    TTGGGAACAC TGGAAGGTCC TGATCCAACA GCCCCTGAAT CACCTGTGTA CACTGGGCCA
    GAGGTGACGG AAACAGAGAC AGCGGTATAC AGAGAGGGAC TTGGGATAAG AGACAGCAGC
    ATCCCTCCAG CATACAGGCA CTTACTAGAT GGGAAAGTAG ATGGAAAAGA TGCACCCCCA
    CCACCGCCAG AGGAAAAGCC TCTGACCGAT GATCAGCTGA TTGATAAGTT TTCTATGGAC
    TTTGATTGTT CTGAATCTCC TGCAACACAA CAGGCTCATC CTGTCGAACC TAAAGTTTCC
    TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT
    GCACCCACTC CTTCTAAAGC ACCAGCACCT CCAGCTGATC CACTCGATAG CCTGTCTGGT
    ACCTTAGGTG TTCGAAAGGA AGAACCGAGT GACAAGAAGC CTCCAGTGGA TAAAGTAAAG
    GAGGGAACAG GCAAAGAGAA GAAAGAAAAG CTTGGAGAAG ATGAAAAAAC AATACATCCT
    GACCGCCGGC TAAAGGAAGT GAAGGATAAA GACGGAAAGC CCTTGCTGCC AAAACTTGAA
    CAAAGCCCTA AGCCAATGAG TGAGGCCGAG CTGCTGGATG CTTTGACTGA AGGCTTTGTG
    ACTTCCCCTG CCACTCCTGA TGCCCCACTG CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA
    TCAGAAGATG TTATATCTTG TTCTAAAGCT TCCGCCGTCA AGTCTGGAGC TCCACAGGAC
    GCTGCTTTAG ATATACAGAT TCCCGACGAT GCACTAGATT TACTTTCTGG CTCCCTGGGA
    CAAAGAGAGG TGGATCCTAA TGAAAACAAA CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT
    AAAGCTGAGC ATATAGACAA ACTGGGGGAC AGAGATGATA CAATCCCACC GGAATACAGA
    AAGCTGCTGG ATGGTAAAGA TGATAAGGGA CAACCTGCAA AACCACCAGC GAAAGCGGAG
    GAGAAACCAA AGAAGCCTTT AAGTGACGAT GCAGCGATCG ATGCTCTTTC AAGCGGATTT
    GCATCATGTG ACACAAAATC TACTGAACAA CCAAAGCAGC CTTCAACGGA CAAGACTGAG
    AAACAATGTG CTAGCGCTCC AGTGTCGCAG CCTTCAGGAA AGGCACCTGA TGCTGCAAAG
    CAGGCAAACT CAGCGATACC AAGTGTGCCA AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811542.1
    CDS210..3137
    Translation

    Target ORF information:

    RefSeq Version XM_012956088.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956088.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
    CCATCAGAAG GAGCAGAGAC TCCTAGTGCT TCAGGAAAAA CTATGAACCC GGCGGATACA
    CAGGCTAAAC AAACACCCAA GACTGAACCA GCCAAATCTA CAAAGCCAAC AAAGCCAAAA
    GAAGCAACAA CAGCATCTTC AGCAGCCCTG GCGGCTGCAT CCAAACCAAC AGCAGCACCT
    TCCTCAGCCC CAGTAACAGC AACCAAACCA ACAAAAACAA AGCCAAGTGC AGCAAAATCG
    CCTCCCAAAC AAGATATAAA GCCTATCCCT GGGAAGCAGC AAAAAACAGA CAGCAAGAGT
    AAAGATGAAA AGAAAAGTGG TGATAAAGTT TCTCCAGCTG TGCTACCTAA GATGACACCT
    AGTTCTGCTG GTAAAGTATC TGCAGTTGCC ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC
    GCTGCAGTCA CTGCTGTGCA GTCAACAAAG CCGAAAGATG AGAGTAAAGA GGAAAAGAAA
    GCCATTACTG GGGATAAGGT TTCTTCTGGG CCACCGGCCA AGACAGACGC AGATGCAGCC
    TTGGACGAAC TACTTGGAAC GTTAGAGGGC CCAGCAGATA TTCCAGAGTC TCCACAATTT
    ACTGGACCAG AAATCCAGGA CACAGTTGTT ACATCTGAGT ATTTGGAGGA GCTAGGAAAA
    CGGGATTTCA CCATCCCACC TGAATACAGG CACCTGCTGG ATGGTAAAGG TGAAAAGATG
    GCACCCCCTA CCCCACCCGA GGAACCCGAA GAGCACATGG ATGATGATGA ATTAGTTGCA
    GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG GCATCCCCCG AAGTAAAGAA GCCAAAGCTG
    GAAGAGAAAA TGGAAGTGAA ACCAAAATCA GCAGCAGCAG TGGCCACACA GCCAGATGTG
    CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA GAAACTTCCA AACCAAGCAC TCAGAAAAAA
    GAGGAAAAAG CAAAAATAGC AGAAGTGTCT AAACCAAGCA CTCAGAAAAA AGAGGAAAAA
    CTGGCAGCAG CAGCAGACAC ATCCAAACCA AGCACTCAGA AAAAAGAGGT AAAAGCCACA
    GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA CAAAGCACTG AGTCCATACC ATCAGAAGCA
    ATAGACGAGT TGTTGGGAAC ACTTGAAGGA CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA
    TTTCAGGGTC CAGAAGTTAC TGAGAACATT ACAGCAACAT ACTTAGAAGA AACTGGAAAA
    AGAGATAGTA CGATCCCTCC AGAATACAGA AAATTATTAG ATGGAAAGGA TCATGGGAAA
    GATGTGTGGC CTTCAGCACC TGAACCAGAA CCGTCTATGA CCGACACAGA GCTTGTAGAT
    GTGTTTTCCA AAGACTTTGA GCTTTCATGT TCACCTACTG TTCAGACCAC GCCAGCCATT
    AAACCCAAAA GGAACACTGA AGATCTCCAC GAGTGCTATT GCTCAAAGTC AGTGAGCCAA
    CTTTCATCAT CTGAAACTCC TCAAGGTAAC AAAACGAAAC CTGAAATTGC GGCATCATCT
    GCATCCACTG TAAAAGCAGC AGCGCCCTCC GGTGGTATGG ACAGTGCATT AGATGAATTA
    TTGGGAACAC TGGAAGGTCC TGATCCAACA GCCCCTGAAT CACCTGTGTA CACTGGGCCA
    GAGGTGACGG AAACAGAGAC AGCGGTATAC AGAGAGGGAC TTGGGATAAG AGACAGCAGC
    ATCCCTCCAG CATACAGGCA CTTACTAGAT GGGAAAGTAG ATGGAAAAGA TGCACCCCCA
    CCACCGCCAG AGGAAAAGCC TCTGACCGAT GATCAGCTGA TTGATAAGTT TTCTATGGAC
    TTTGATTGTT CTGAATCTCC TGCAACACAA CAGGCTCATC CTGTCGAACC TAAAGTTTCC
    TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT
    GCACCCACTC CTTCTAAAGC ACCAGCACCT CCAGCTGATC CACTCGATAG CCTGTCTGGT
    ACCTTAGGTG TTCGAAAGGA AGAACCGAGT GACAAGAAGC CTCCAGTGGA TAAAGTAAAG
    GAGGGAACAG GCAAAGAGAA GAAAGAAAAG CTTGGAGAAG ATGAAAAAAC AATACATCCT
    GACCGCCGGC TAAAGGAAGT GAAGGATAAA GACGGAAAGC CCTTGCTGCC AAAACTTGAA
    CAAAGCCCTA AGCCAATGAG TGAGGCCGAG CTGCTGGATG CTTTGACTGA AGGCTTTGTG
    ACTTCCCCTG CCACTCCTGA TGCCCCACTG CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA
    TCAGAAGATG TTATATCTTG TTCTAAAGCT TCCGCCGTCA AGTCTGGAGC TCCACAGGAC
    GCTGCTTTAG ATATACAGAT TCCCGACGAT GCACTAGATT TACTTTCTGG CTCCCTGGGA
    CAAAGAGAGG TGGATCCTAA TGAAAACAAA CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT
    AAAGCTGAGC ATATAGACAA ACTGGGGGAC AGAGATGATA CAATCCCACC GGAATACAGA
    AAGCTGCTGG ATGGTAAAGA TGATAAGGGA CAACCTGCAA AACCACCAGC GAAAGCGGAG
    GAGAAACCAA AGAAGCCTTT AAGTGACGAT GCAGCGATCG ATGCTCTTTC AAGCGGATTT
    GCATCATGTG ACACAAAATC TACTGAACAA CCAAAGCAGC CTTCAACGGA CAAGACTGAG
    AAACAATGTG CTAGCGCTCC AGTGTCGCAG CCTTCAGGAA AGGCACCTGA TGCTGCAAAG
    CAGGCAAACT CAGCGATACC AAGTGTGCCA AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31735
    Clone ID Related Accession (Same CDS sequence) XM_012956093.1
    Accession Version XM_012956093.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
    CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGGCTAAACA AACACCCAAG
    ACTGAACCAG CCAAATCTAC AAAGCCAACA AAGCCAAAAG AAGCAACAAC AGCATCTTCA
    GCAGCCCTGG CGGCTGCATC CAAACCAACA GCAGCACCTT CCTCAGCCCC AGTAACAGCA
    ACCAAACCAA CAAAAACAAA GCCAAGTGCA GCAAAATCGC CTCCCAAACA AGATATAAAG
    CCTATCCCTG GGAAGCAGCA AAAAACAGAC AGCAAGAGTA AAGATGAAAA GAAAAGTGGT
    GATAAAGTTT CTCCAGCTGT GCTACCTAAG ATGACACCTA GTTCTGCTGG TAAAGTATCT
    GCAGTTGCCA CTGGAGCTCT AGCAGCTGCT GGTGCCGCCG CTGCAGTCAC TGCTGTGCAG
    TCAACAAAGC CGAAAGATGA GAGTAAAGAG GAAAAGAAAG CCATTACTGG GGATAAGGTT
    TCTTCTGGGC CACCGGCCAA GACAGACGCA GATGCAGCCT TGGACGAACT ACTTGGAACG
    TTAGAGGGCC CAGCAGATAT TCCAGAGTCT CCACAATTTA CTGGACCAGA AATCCAGGAC
    ACAGTTGTTA CATCTGAGTA TTTGGAGGAG CTAGGAAAAC GGGATTTCAC CATCCCACCT
    GAATACAGGC ACCTGCTGGA TGGTAAAGGT GAAAAGATGG CACCCCCTAC CCCACCCGAG
    GAACCCGAAG AGCACATGGA TGATGATGAA TTAGTTGCAG CCCTGTCTTC TGGTTTTGAG
    AGCAGTCAGG CATCCCCCGA AGTAAAGAAG CCAAAGCTGG AAGAGAAAAT GGAAGTGAAA
    CCAAAATCAG CAGCAGCAGT GGCCACACAG CCAGATGTGC AGAAAAAAGA AGTAAAAGTC
    GCAGCAGCAG AAACTTCCAA ACCAAGCACT CAGAAAAAAG AGGAAAAAGC AAAAATAGCA
    GAAGTGTCTA AACCAAGCAC TCAGAAAAAA GAGGAAAAAC TGGCAGCAGC AGCAGACACA
    TCCAAACCAA GCACTCAGAA AAAAGAGGTA AAAGCCACAG CAAAAGCCAC AGCTGCCGAA
    GCTTCCAAAC AAAGCACTGA GTCCATACCA TCAGAAGCAA TAGACGAGTT GTTGGGAACA
    CTTGAAGGAC CCCCAGAAAA TGTTCCAGGG TCTCCAGTAT TTCAGGGTCC AGAAGTTACT
    GAGAACATTA CAGCAACATA CTTAGAAGAA ACTGGAAAAA GAGATAGTAC GATCCCTCCA
    GAATACAGAA AATTATTAGA TGGAAAGGAT CATGGGAAAG ATGTGTGGCC TTCAGCACCT
    GAACCAGAAC CGTCTATGAC CGACACAGAG CTTGTAGATG TGTTTTCCAA AGACTTTGAG
    CTTTCATGTT CACCTACTGT TCAGACCACG CCAGCCATTA AACCCAAAAG GAACACTGAA
    GATCTCCACG AGTGCTATTG CTCAAAGTCA GTGAGCCAAC TTTCATCATC TGAAACTCCT
    CAAGGTAACA AAACGAAACC TGAAATTGCG GCATCATCTG CATCCACTGT AAAAGCAGCA
    GCGCCCTCCG GTGGTATGGA CAGTGCATTA GATGAATTAT TGGGAACACT GGAAGGTCCT
    GATCCAACAG CCCCTGAATC ACCTGTGTAC ACTGGGCCAG AGGTGACGGA AACAGAGACA
    GCGGTATACA GAGAGGGACT TGGGATAAGA GACAGCAGCA TCCCTCCAGC ATACAGGCAC
    TTACTAGATG GGAAAGTAGA TGGAAAAGAT GCACCCCCAC CACCGCCAGA GGAAAAGCCT
    CTGACCGATG ATCAGCTGAT TGATAAGTTT TCTATGGACT TTGATTGTTC TGAATCTCCT
    GCAACACAAC AGGCTCATCC TGTCGAACCT AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA
    AAAGAAGTAG TTGCTGCCTC CTCTGCTACT GTTAAAGCTG CACCCACTCC TTCTAAAGCA
    CCAGCACCTC CAGCTGATCC ACTCGATAGC CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA
    GAACCGAGTG ACAAGAAGCC TCCAGTGGAT AAAGTAAAGG AGGGAACAGG CAAAGAGAAG
    AAAGAAAAGC TTGGAGAAGA TGAAAAAACA ATACATCCTG ACCGCCGGCT AAAGGAAGTG
    AAGGATAAAG ACGGAAAGCC CTTGCTGCCA AAACTTGAAC AAAGCCCTAA GCCAATGAGT
    GAGGCCGAGC TGCTGGATGC TTTGACTGAA GGCTTTGTGA CTTCCCCTGC CACTCCTGAT
    GCCCCACTGC AGGGCTCTTT GAAAAAAAAA GGTTCTGGAT CAGAAGATGT TATATCTTGT
    TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT CCACAGGACG CTGCTTTAGA TATACAGATT
    CCCGACGATG CACTAGATTT ACTTTCTGGC TCCCTGGGAC AAAGAGAGGT GGATCCTAAT
    GAAAACAAAC CGGTGGTGGA TGTAGTTAAG GAAAAAGCTA AAGCTGAGCA TATAGACAAA
    CTGGGGGACA GAGATGATAC AATCCCACCG GAATACAGAA AGCTGCTGGA TGGTAAAGAT
    GATAAGGGAC AACCTGCAAA ACCACCAGCG AAAGCGGAGG AGAAACCAAA GAAGCCTTTA
    AGTGACGATG CAGCGATCGA TGCTCTTTCA AGCGGATTTG CATCATGTGA CACAAAATCT
    ACTGAACAAC CAAAGCAGCC TTCAACGGAC AAGACTGAGA AACAATGTGC TAGCGCTCCA
    GTGTCGCAGC CTTCAGGAAA GGCACCTGAT GCTGCAAAGC AGGCAAACTC AGCGATACCA
    AGTGTGCCAA AGGCTGGCAA AAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811547.1
    CDS206..3112
    Translation

    Target ORF information:

    RefSeq Version XM_012956093.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956093.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
    CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGGCTAAACA AACACCCAAG
    ACTGAACCAG CCAAATCTAC AAAGCCAACA AAGCCAAAAG AAGCAACAAC AGCATCTTCA
    GCAGCCCTGG CGGCTGCATC CAAACCAACA GCAGCACCTT CCTCAGCCCC AGTAACAGCA
    ACCAAACCAA CAAAAACAAA GCCAAGTGCA GCAAAATCGC CTCCCAAACA AGATATAAAG
    CCTATCCCTG GGAAGCAGCA AAAAACAGAC AGCAAGAGTA AAGATGAAAA GAAAAGTGGT
    GATAAAGTTT CTCCAGCTGT GCTACCTAAG ATGACACCTA GTTCTGCTGG TAAAGTATCT
    GCAGTTGCCA CTGGAGCTCT AGCAGCTGCT GGTGCCGCCG CTGCAGTCAC TGCTGTGCAG
    TCAACAAAGC CGAAAGATGA GAGTAAAGAG GAAAAGAAAG CCATTACTGG GGATAAGGTT
    TCTTCTGGGC CACCGGCCAA GACAGACGCA GATGCAGCCT TGGACGAACT ACTTGGAACG
    TTAGAGGGCC CAGCAGATAT TCCAGAGTCT CCACAATTTA CTGGACCAGA AATCCAGGAC
    ACAGTTGTTA CATCTGAGTA TTTGGAGGAG CTAGGAAAAC GGGATTTCAC CATCCCACCT
    GAATACAGGC ACCTGCTGGA TGGTAAAGGT GAAAAGATGG CACCCCCTAC CCCACCCGAG
    GAACCCGAAG AGCACATGGA TGATGATGAA TTAGTTGCAG CCCTGTCTTC TGGTTTTGAG
    AGCAGTCAGG CATCCCCCGA AGTAAAGAAG CCAAAGCTGG AAGAGAAAAT GGAAGTGAAA
    CCAAAATCAG CAGCAGCAGT GGCCACACAG CCAGATGTGC AGAAAAAAGA AGTAAAAGTC
    GCAGCAGCAG AAACTTCCAA ACCAAGCACT CAGAAAAAAG AGGAAAAAGC AAAAATAGCA
    GAAGTGTCTA AACCAAGCAC TCAGAAAAAA GAGGAAAAAC TGGCAGCAGC AGCAGACACA
    TCCAAACCAA GCACTCAGAA AAAAGAGGTA AAAGCCACAG CAAAAGCCAC AGCTGCCGAA
    GCTTCCAAAC AAAGCACTGA GTCCATACCA TCAGAAGCAA TAGACGAGTT GTTGGGAACA
    CTTGAAGGAC CCCCAGAAAA TGTTCCAGGG TCTCCAGTAT TTCAGGGTCC AGAAGTTACT
    GAGAACATTA CAGCAACATA CTTAGAAGAA ACTGGAAAAA GAGATAGTAC GATCCCTCCA
    GAATACAGAA AATTATTAGA TGGAAAGGAT CATGGGAAAG ATGTGTGGCC TTCAGCACCT
    GAACCAGAAC CGTCTATGAC CGACACAGAG CTTGTAGATG TGTTTTCCAA AGACTTTGAG
    CTTTCATGTT CACCTACTGT TCAGACCACG CCAGCCATTA AACCCAAAAG GAACACTGAA
    GATCTCCACG AGTGCTATTG CTCAAAGTCA GTGAGCCAAC TTTCATCATC TGAAACTCCT
    CAAGGTAACA AAACGAAACC TGAAATTGCG GCATCATCTG CATCCACTGT AAAAGCAGCA
    GCGCCCTCCG GTGGTATGGA CAGTGCATTA GATGAATTAT TGGGAACACT GGAAGGTCCT
    GATCCAACAG CCCCTGAATC ACCTGTGTAC ACTGGGCCAG AGGTGACGGA AACAGAGACA
    GCGGTATACA GAGAGGGACT TGGGATAAGA GACAGCAGCA TCCCTCCAGC ATACAGGCAC
    TTACTAGATG GGAAAGTAGA TGGAAAAGAT GCACCCCCAC CACCGCCAGA GGAAAAGCCT
    CTGACCGATG ATCAGCTGAT TGATAAGTTT TCTATGGACT TTGATTGTTC TGAATCTCCT
    GCAACACAAC AGGCTCATCC TGTCGAACCT AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA
    AAAGAAGTAG TTGCTGCCTC CTCTGCTACT GTTAAAGCTG CACCCACTCC TTCTAAAGCA
    CCAGCACCTC CAGCTGATCC ACTCGATAGC CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA
    GAACCGAGTG ACAAGAAGCC TCCAGTGGAT AAAGTAAAGG AGGGAACAGG CAAAGAGAAG
    AAAGAAAAGC TTGGAGAAGA TGAAAAAACA ATACATCCTG ACCGCCGGCT AAAGGAAGTG
    AAGGATAAAG ACGGAAAGCC CTTGCTGCCA AAACTTGAAC AAAGCCCTAA GCCAATGAGT
    GAGGCCGAGC TGCTGGATGC TTTGACTGAA GGCTTTGTGA CTTCCCCTGC CACTCCTGAT
    GCCCCACTGC AGGGCTCTTT GAAAAAAAAA GGTTCTGGAT CAGAAGATGT TATATCTTGT
    TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT CCACAGGACG CTGCTTTAGA TATACAGATT
    CCCGACGATG CACTAGATTT ACTTTCTGGC TCCCTGGGAC AAAGAGAGGT GGATCCTAAT
    GAAAACAAAC CGGTGGTGGA TGTAGTTAAG GAAAAAGCTA AAGCTGAGCA TATAGACAAA
    CTGGGGGACA GAGATGATAC AATCCCACCG GAATACAGAA AGCTGCTGGA TGGTAAAGAT
    GATAAGGGAC AACCTGCAAA ACCACCAGCG AAAGCGGAGG AGAAACCAAA GAAGCCTTTA
    AGTGACGATG CAGCGATCGA TGCTCTTTCA AGCGGATTTG CATCATGTGA CACAAAATCT
    ACTGAACAAC CAAAGCAGCC TTCAACGGAC AAGACTGAGA AACAATGTGC TAGCGCTCCA
    GTGTCGCAGC CTTCAGGAAA GGCACCTGAT GCTGCAAAGC AGGCAAACTC AGCGATACCA
    AGTGTGCCAA AGGCTGGCAA AAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31737
    Clone ID Related Accession (Same CDS sequence) XM_012956106.2
    Accession Version XM_012956106.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
    GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG
    CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCTGGCGG CTGCATCCAA ACCAACAGCA
    GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA
    AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC
    AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGATG
    ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT
    GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA
    AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT
    GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA
    CAATTTACTG GACCAGAAAT CCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA
    GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA
    AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA
    GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA
    AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA
    GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG
    AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG
    GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA
    GCCACAGCAA AAGCCACAGC TGCCGAAGCT TCCAAACAAA GCACTGAGTC CATACCATCA
    GAAGCAATAG ACGAGTTGTT GGGAACACTT GAAGGACCCC CAGAAAATGT TCCAGGGTCT
    CCAGTATTTC AGGGTCCAGA AGTTACTGAG AACATTACAG CAACATACTT AGAAGAAACT
    GGAAAAAGAG ATAGTACGAT CCCTCCAGAA TACAGAAAAT TATTAGATGG AAAGGATCAT
    GGGAAAGATG TGTGGCCTTC AGCACCTGAA CCAGAACCGT CTATGACCGA CACAGAGCTT
    GTAGATGTGT TTTCCAAAGA CTTTGAGCTT TCATGTTCAC CTACTGTTCA GACCACGCCA
    GCCATTAAAC CCAAAAGGAA CACTGAAGAT CTCCACGAGT GCTATTGCTC AAAGTCAGTG
    AGCCAACTTT CATCATCTGA AACTCCTCAA GGTAACAAAA CGAAACCTGA AATTGCGGCA
    TCATCTGCAT CCACTGTAAA AGCAGCAGCG CCCTCCGGTG GTATGGACAG TGCATTAGAT
    GAATTATTGG GAACACTGGA AGGTCCTGAT CCAACAGCCC CTGAATCACC TGTGTACACT
    GGGCCAGAGG TGACGGAAAC AGAGACAGCG GTATACAGAG AGGGACTTGG GATAAGAGAC
    AGCAGCATCC CTCCAGCATA CAGGCACTTA CTAGATGGGA AAGTAGATGG AAAAGATGCA
    CCCCCACCAC CGCCAGAGGA AAAGCCTCTG ACCGATGATC AGCTGATTGA TAAGTTTTCT
    ATGGACTTTG ATTGTTCTGA ATCTCCTGCA ACACAACAGG CTCATCCTGT CGAACCTAAA
    GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA GAAGTAGTTG CTGCCTCCTC TGCTACTGTT
    AAAGCTGCAC CCACTCCTTC TAAAGCACCA GCACCTCCAG CTGATCCACT CGATAGCCTG
    TCTGGTACCT TAGGTGTTCG AAAGGAAGAA CCGAGTGACA AGAAGCCTCC AGTGGATAAA
    GTAAAGGAGG GAACAGGCAA AGAGAAGAAA GAAAAGCTTG GAGAAGATGA AAAAACAATA
    CATCCTGACC GCCGGCTAAA GGAAGTGAAG GATAAAGACG GAAAGCCCTT GCTGCCAAAA
    CTTGAACAAA GCCCTAAGCC AATGAGTGAG GCCGAGCTGC TGGATGCTTT GACTGAAGGC
    TTTGTGACTT CCCCTGCCAC TCCTGATGCC CCACTGCAGG GCTCTTTGAA AAAAAAAGGT
    TCTGGATCAG AAGATGTTAT ATCTTGTTCT AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA
    CAGGACGCTG CTTTAGATAT ACAGATTCCC GACGATGCAC TAGATTTACT TTCTGGCTCC
    CTGGGACAAA GAGAGGTGGA TCCTAATGAA AACAAACCGG TGGTGGATGT AGTTAAGGAA
    AAAGCTAAAG CTGAGCATAT AGACAAACTG GGGGACAGAG ATGATACAAT CCCACCGGAA
    TACAGAAAGC TGCTGGATGG TAAAGATGAT AAGGGACAAC CTGCAAAACC ACCAGCGAAA
    GCGGAGGAGA AACCAAAGAA GCCTTTAAGT GACGATGCAG CGATCGATGC TCTTTCAAGC
    GGATTTGCAT CATGTGACAC AAAATCTACT GAACAACCAA AGCAGCCTTC AACGGACAAG
    ACTGAGAAAC AATGTGCTAG CGCTCCAGTG TCGCAGCCTT CAGGAAAGGC ACCTGATGCT
    GCAAAGCAGG CAAACTCAGC GATACCAAGT GTGCCAAAGG CTGGCAAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811560.1
    CDS154..3027
    Translation

    Target ORF information:

    RefSeq Version XM_012956106.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
    GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG
    CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCTGGCGG CTGCATCCAA ACCAACAGCA
    GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA
    AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC
    AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGATG
    ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT
    GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA
    AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT
    GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA
    CAATTTACTG GACCAGAAAT CCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA
    GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA
    AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA
    GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA
    AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA
    GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG
    AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG
    GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA
    GCCACAGCAA AAGCCACAGC TGCCGAAGCT TCCAAACAAA GCACTGAGTC CATACCATCA
    GAAGCAATAG ACGAGTTGTT GGGAACACTT GAAGGACCCC CAGAAAATGT TCCAGGGTCT
    CCAGTATTTC AGGGTCCAGA AGTTACTGAG AACATTACAG CAACATACTT AGAAGAAACT
    GGAAAAAGAG ATAGTACGAT CCCTCCAGAA TACAGAAAAT TATTAGATGG AAAGGATCAT
    GGGAAAGATG TGTGGCCTTC AGCACCTGAA CCAGAACCGT CTATGACCGA CACAGAGCTT
    GTAGATGTGT TTTCCAAAGA CTTTGAGCTT TCATGTTCAC CTACTGTTCA GACCACGCCA
    GCCATTAAAC CCAAAAGGAA CACTGAAGAT CTCCACGAGT GCTATTGCTC AAAGTCAGTG
    AGCCAACTTT CATCATCTGA AACTCCTCAA GGTAACAAAA CGAAACCTGA AATTGCGGCA
    TCATCTGCAT CCACTGTAAA AGCAGCAGCG CCCTCCGGTG GTATGGACAG TGCATTAGAT
    GAATTATTGG GAACACTGGA AGGTCCTGAT CCAACAGCCC CTGAATCACC TGTGTACACT
    GGGCCAGAGG TGACGGAAAC AGAGACAGCG GTATACAGAG AGGGACTTGG GATAAGAGAC
    AGCAGCATCC CTCCAGCATA CAGGCACTTA CTAGATGGGA AAGTAGATGG AAAAGATGCA
    CCCCCACCAC CGCCAGAGGA AAAGCCTCTG ACCGATGATC AGCTGATTGA TAAGTTTTCT
    ATGGACTTTG ATTGTTCTGA ATCTCCTGCA ACACAACAGG CTCATCCTGT CGAACCTAAA
    GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA GAAGTAGTTG CTGCCTCCTC TGCTACTGTT
    AAAGCTGCAC CCACTCCTTC TAAAGCACCA GCACCTCCAG CTGATCCACT CGATAGCCTG
    TCTGGTACCT TAGGTGTTCG AAAGGAAGAA CCGAGTGACA AGAAGCCTCC AGTGGATAAA
    GTAAAGGAGG GAACAGGCAA AGAGAAGAAA GAAAAGCTTG GAGAAGATGA AAAAACAATA
    CATCCTGACC GCCGGCTAAA GGAAGTGAAG GATAAAGACG GAAAGCCCTT GCTGCCAAAA
    CTTGAACAAA GCCCTAAGCC AATGAGTGAG GCCGAGCTGC TGGATGCTTT GACTGAAGGC
    TTTGTGACTT CCCCTGCCAC TCCTGATGCC CCACTGCAGG GCTCTTTGAA AAAAAAAGGT
    TCTGGATCAG AAGATGTTAT ATCTTGTTCT AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA
    CAGGACGCTG CTTTAGATAT ACAGATTCCC GACGATGCAC TAGATTTACT TTCTGGCTCC
    CTGGGACAAA GAGAGGTGGA TCCTAATGAA AACAAACCGG TGGTGGATGT AGTTAAGGAA
    AAAGCTAAAG CTGAGCATAT AGACAAACTG GGGGACAGAG ATGATACAAT CCCACCGGAA
    TACAGAAAGC TGCTGGATGG TAAAGATGAT AAGGGACAAC CTGCAAAACC ACCAGCGAAA
    GCGGAGGAGA AACCAAAGAA GCCTTTAAGT GACGATGCAG CGATCGATGC TCTTTCAAGC
    GGATTTGCAT CATGTGACAC AAAATCTACT GAACAACCAA AGCAGCCTTC AACGGACAAG
    ACTGAGAAAC AATGTGCTAG CGCTCCAGTG TCGCAGCCTT CAGGAAAGGC ACCTGATGCT
    GCAAAGCAGG CAAACTCAGC GATACCAAGT GTGCCAAAGG CTGGCAAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31739
    Clone ID Related Accession (Same CDS sequence) XM_012956118.1
    Accession Version XM_012956118.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG
    CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA
    GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG
    AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG
    ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT
    CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT
    TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT
    GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT
    GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA
    GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG
    GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT
    CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA
    AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG
    TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT
    GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC
    TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT
    GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC
    GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT
    CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC
    TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811572.1
    CDS206..3031
    Translation

    Target ORF information:

    RefSeq Version XM_012956118.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956118.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG
    CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA
    GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG
    AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG
    ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT
    CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT
    TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT
    GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT
    GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA
    GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG
    GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT
    CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA
    AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG
    TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT
    GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC
    TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT
    GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC
    GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT
    CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC
    TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31740
    Clone ID Related Accession (Same CDS sequence) XM_012956125.1
    Accession Version XM_012956125.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC
    AAATCTACAA AGCCAACAAA GAGTAAAGAT GAAAAGAAAA GTGGTGATAA AGTTTCTCCA
    GCTGTGCTAC CTAAGATGAC ACCTAGTTCT GCTGGTAAAG TATCTGCAGT TGCCACTGGA
    GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA GTCACTGCTG TGCAGTCAAC AAAGCCGAAA
    GATGAGAGTA AAGAGGAAAA GAAAGCCATT ACTGGGGATA AGGTTTCTTC TGGGCCACCG
    GCCAAGACAG ACGCAGATGC AGCCTTGGAC GAACTACTTG GAACGTTAGA GGGCCCAGCA
    GATATTCCAG AGTCTCCACA ATTTACTGGA CCAGAAATCC AGGACACAGT TGTTACATCT
    GAGTATTTGG AGGAGCTAGG AAAACGGGAT TTCACCATCC CACCTGAATA CAGGCACCTG
    CTGGATGGTA AAGGTGAAAA GATGGCACCC CCTACCCCAC CCGAGGAACC CGAAGAGCAC
    ATGGATGATG ATGAATTAGT TGCAGCCCTG TCTTCTGGTT TTGAGAGCAG TCAGGCATCC
    CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG AAAATGGAAG TGAAACCAAA ATCAGCAGCA
    GCAGTGGCCA CACAGCCAGA TGTGCAGAAA AAAGAAGTAA AAGTCGCAGC AGCAGAAACT
    TCCAAACCAA GCACTCAGAA AAAAGAGGAA AAAGCAAAAA TAGCAGAAGT GTCTAAACCA
    AGCACTCAGA AAAAAGAGGA AAAACTGGCA GCAGCAGCAG ACACATCCAA ACCAAGCACT
    CAGAAAAAAG AGGTAAAAGC CACAGCAAAA GCCACAGCTG CCGAAGCTTC CAAACAAAGC
    ACTGAGTCCA TACCATCAGA AGCAATAGAC GAGTTGTTGG GAACACTTGA AGGACCCCCA
    GAAAATGTTC CAGGGTCTCC AGTATTTCAG GGTCCAGAAG TTACTGAGAA CATTACAGCA
    ACATACTTAG AAGAAACTGG AAAAAGAGAT AGTACGATCC CTCCAGAATA CAGAAAATTA
    TTAGATGGAA AGGATCATGG GAAAGATGTG TGGCCTTCAG CACCTGAACC AGAACCGTCT
    ATGACCGACA CAGAGCTTGT AGATGTGTTT TCCAAAGACT TTGAGCTTTC ATGTTCACCT
    ACTGTTCAGA CCACGCCAGC CATTAAACCC AAAAGGAACA CTGAAGATCT CCACGAGTGC
    TATTGCTCAA AGTCAGTGAG CCAACTTTCA TCATCTGAAA CTCCTCAAGG TAACAAAACG
    AAACCTGAAA TTGCGGCATC ATCTGCATCC ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT
    ATGGACAGTG CATTAGATGA ATTATTGGGA ACACTGGAAG GTCCTGATCC AACAGCCCCT
    GAATCACCTG TGTACACTGG GCCAGAGGTG ACGGAAACAG AGACAGCGGT ATACAGAGAG
    GGACTTGGGA TAAGAGACAG CAGCATCCCT CCAGCATACA GGCACTTACT AGATGGGAAA
    GTAGATGGAA AAGATGCACC CCCACCACCG CCAGAGGAAA AGCCTCTGAC CGATGATCAG
    CTGATTGATA AGTTTTCTAT GGACTTTGAT TGTTCTGAAT CTCCTGCAAC ACAACAGGCT
    CATCCTGTCG AACCTAAAGT TTCCTCGCAA GAGAAAAAGG CAGAAAAAGA AGTAGTTGCT
    GCCTCCTCTG CTACTGTTAA AGCTGCACCC ACTCCTTCTA AAGCACCAGC ACCTCCAGCT
    GATCCACTCG ATAGCCTGTC TGGTACCTTA GGTGTTCGAA AGGAAGAACC GAGTGACAAG
    AAGCCTCCAG TGGATAAAGT AAAGGAGGGA ACAGGCAAAG AGAAGAAAGA AAAGCTTGGA
    GAAGATGAAA AAACAATACA TCCTGACCGC CGGCTAAAGG AAGTGAAGGA TAAAGACGGA
    AAGCCCTTGC TGCCAAAACT TGAACAAAGC CCTAAGCCAA TGAGTGAGGC CGAGCTGCTG
    GATGCTTTGA CTGAAGGCTT TGTGACTTCC CCTGCCACTC CTGATGCCCC ACTGCAGGGC
    TCTTTGAAAA AAAAAGGTTC TGGATCAGAA GATGTTATAT CTTGTTCTAA AGCTTCCGCC
    GTCAAGTCTG GAGCTCCACA GGACGCTGCT TTAGATATAC AGATTCCCGA CGATGCACTA
    GATTTACTTT CTGGCTCCCT GGGACAAAGA GAGGTGGATC CTAATGAAAA CAAACCGGTG
    GTGGATGTAG TTAAGGAAAA AGCTAAAGCT GAGCATATAG ACAAACTGGG GGACAGAGAT
    GATACAATCC CACCGGAATA CAGAAAGCTG CTGGATGGTA AAGATGATAA GGGACAACCT
    GCAAAACCAC CAGCGAAAGC GGAGGAGAAA CCAAAGAAGC CTTTAAGTGA CGATGCAGCG
    ATCGATGCTC TTTCAAGCGG ATTTGCATCA TGTGACACAA AATCTACTGA ACAACCAAAG
    CAGCCTTCAA CGGACAAGAC TGAGAAACAA TGTGCTAGCG CTCCAGTGTC GCAGCCTTCA
    GGAAAGGCAC CTGATGCTGC AAAGCAGGCA AACTCAGCGA TACCAAGTGT GCCAAAGGCT
    GGCAAAAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811579.1
    CDS229..3000
    Translation

    Target ORF information:

    RefSeq Version XM_012956125.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956125.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC
    AAATCTACAA AGCCAACAAA GAGTAAAGAT GAAAAGAAAA GTGGTGATAA AGTTTCTCCA
    GCTGTGCTAC CTAAGATGAC ACCTAGTTCT GCTGGTAAAG TATCTGCAGT TGCCACTGGA
    GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA GTCACTGCTG TGCAGTCAAC AAAGCCGAAA
    GATGAGAGTA AAGAGGAAAA GAAAGCCATT ACTGGGGATA AGGTTTCTTC TGGGCCACCG
    GCCAAGACAG ACGCAGATGC AGCCTTGGAC GAACTACTTG GAACGTTAGA GGGCCCAGCA
    GATATTCCAG AGTCTCCACA ATTTACTGGA CCAGAAATCC AGGACACAGT TGTTACATCT
    GAGTATTTGG AGGAGCTAGG AAAACGGGAT TTCACCATCC CACCTGAATA CAGGCACCTG
    CTGGATGGTA AAGGTGAAAA GATGGCACCC CCTACCCCAC CCGAGGAACC CGAAGAGCAC
    ATGGATGATG ATGAATTAGT TGCAGCCCTG TCTTCTGGTT TTGAGAGCAG TCAGGCATCC
    CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG AAAATGGAAG TGAAACCAAA ATCAGCAGCA
    GCAGTGGCCA CACAGCCAGA TGTGCAGAAA AAAGAAGTAA AAGTCGCAGC AGCAGAAACT
    TCCAAACCAA GCACTCAGAA AAAAGAGGAA AAAGCAAAAA TAGCAGAAGT GTCTAAACCA
    AGCACTCAGA AAAAAGAGGA AAAACTGGCA GCAGCAGCAG ACACATCCAA ACCAAGCACT
    CAGAAAAAAG AGGTAAAAGC CACAGCAAAA GCCACAGCTG CCGAAGCTTC CAAACAAAGC
    ACTGAGTCCA TACCATCAGA AGCAATAGAC GAGTTGTTGG GAACACTTGA AGGACCCCCA
    GAAAATGTTC CAGGGTCTCC AGTATTTCAG GGTCCAGAAG TTACTGAGAA CATTACAGCA
    ACATACTTAG AAGAAACTGG AAAAAGAGAT AGTACGATCC CTCCAGAATA CAGAAAATTA
    TTAGATGGAA AGGATCATGG GAAAGATGTG TGGCCTTCAG CACCTGAACC AGAACCGTCT
    ATGACCGACA CAGAGCTTGT AGATGTGTTT TCCAAAGACT TTGAGCTTTC ATGTTCACCT
    ACTGTTCAGA CCACGCCAGC CATTAAACCC AAAAGGAACA CTGAAGATCT CCACGAGTGC
    TATTGCTCAA AGTCAGTGAG CCAACTTTCA TCATCTGAAA CTCCTCAAGG TAACAAAACG
    AAACCTGAAA TTGCGGCATC ATCTGCATCC ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT
    ATGGACAGTG CATTAGATGA ATTATTGGGA ACACTGGAAG GTCCTGATCC AACAGCCCCT
    GAATCACCTG TGTACACTGG GCCAGAGGTG ACGGAAACAG AGACAGCGGT ATACAGAGAG
    GGACTTGGGA TAAGAGACAG CAGCATCCCT CCAGCATACA GGCACTTACT AGATGGGAAA
    GTAGATGGAA AAGATGCACC CCCACCACCG CCAGAGGAAA AGCCTCTGAC CGATGATCAG
    CTGATTGATA AGTTTTCTAT GGACTTTGAT TGTTCTGAAT CTCCTGCAAC ACAACAGGCT
    CATCCTGTCG AACCTAAAGT TTCCTCGCAA GAGAAAAAGG CAGAAAAAGA AGTAGTTGCT
    GCCTCCTCTG CTACTGTTAA AGCTGCACCC ACTCCTTCTA AAGCACCAGC ACCTCCAGCT
    GATCCACTCG ATAGCCTGTC TGGTACCTTA GGTGTTCGAA AGGAAGAACC GAGTGACAAG
    AAGCCTCCAG TGGATAAAGT AAAGGAGGGA ACAGGCAAAG AGAAGAAAGA AAAGCTTGGA
    GAAGATGAAA AAACAATACA TCCTGACCGC CGGCTAAAGG AAGTGAAGGA TAAAGACGGA
    AAGCCCTTGC TGCCAAAACT TGAACAAAGC CCTAAGCCAA TGAGTGAGGC CGAGCTGCTG
    GATGCTTTGA CTGAAGGCTT TGTGACTTCC CCTGCCACTC CTGATGCCCC ACTGCAGGGC
    TCTTTGAAAA AAAAAGGTTC TGGATCAGAA GATGTTATAT CTTGTTCTAA AGCTTCCGCC
    GTCAAGTCTG GAGCTCCACA GGACGCTGCT TTAGATATAC AGATTCCCGA CGATGCACTA
    GATTTACTTT CTGGCTCCCT GGGACAAAGA GAGGTGGATC CTAATGAAAA CAAACCGGTG
    GTGGATGTAG TTAAGGAAAA AGCTAAAGCT GAGCATATAG ACAAACTGGG GGACAGAGAT
    GATACAATCC CACCGGAATA CAGAAAGCTG CTGGATGGTA AAGATGATAA GGGACAACCT
    GCAAAACCAC CAGCGAAAGC GGAGGAGAAA CCAAAGAAGC CTTTAAGTGA CGATGCAGCG
    ATCGATGCTC TTTCAAGCGG ATTTGCATCA TGTGACACAA AATCTACTGA ACAACCAAAG
    CAGCCTTCAA CGGACAAGAC TGAGAAACAA TGTGCTAGCG CTCCAGTGTC GCAGCCTTCA
    GGAAAGGCAC CTGATGCTGC AAAGCAGGCA AACTCAGCGA TACCAAGTGT GCCAAAGGCT
    GGCAAAAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31741
    Clone ID Related Accession (Same CDS sequence) XM_012956133.1
    Accession Version XM_012956133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAACCCGG CGGATACACA GGCTAAACAA ACACCCAAGA CTGAACCAGC CAAATCTACA 
    AAGCCAACAA AGCCAAAAGA AGCAACAACA GCATCTTCAG CAGCCCTGGC GGCTGCATCC
    AAACCAACAG CAGCACCTTC CTCAGCCCCA GTAACAGCAA CCAAACCAAC AAAAACAAAG
    CCAAGTGCAG CAAAATCGCC TCCCAAACAA GATATAAAGC CTATCCCTGG GAAGCAGCAA
    AAAACAGACA GCAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG
    CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA
    GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG
    AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG
    ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT
    CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT
    TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT
    GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT
    GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA
    GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG
    GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT
    CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA
    AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG
    TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT
    GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC
    TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT
    GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC
    GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT
    CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC
    TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811587.1
    CDS167..2992
    Translation

    Target ORF information:

    RefSeq Version XM_012956133.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAACCCGG CGGATACACA GGCTAAACAA ACACCCAAGA CTGAACCAGC CAAATCTACA 
    AAGCCAACAA AGCCAAAAGA AGCAACAACA GCATCTTCAG CAGCCCTGGC GGCTGCATCC
    AAACCAACAG CAGCACCTTC CTCAGCCCCA GTAACAGCAA CCAAACCAAC AAAAACAAAG
    CCAAGTGCAG CAAAATCGCC TCCCAAACAA GATATAAAGC CTATCCCTGG GAAGCAGCAA
    AAAACAGACA GCAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG
    CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA
    GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG
    AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG
    ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT
    CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT
    TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT
    GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT
    GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA
    GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG
    GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA
    CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT
    CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA
    AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG
    TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT
    GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC
    TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT
    GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC
    GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT
    CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC
    TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT
    GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC
    AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA
    CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT
    GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT
    GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT
    GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT
    GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC
    TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA
    CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT
    CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT
    GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC
    TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT
    TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG
    AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG
    TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA
    CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT
    GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA
    ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA
    CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT
    GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT
    TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG
    GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41680
    Clone ID Related Accession (Same CDS sequence) XM_018090942.1
    Accession Version XM_018090942.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGCCAAT GAGTGAGGCC
    GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA
    CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA
    GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC
    GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC
    AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG
    GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG
    GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC
    GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA
    CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG
    CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG
    CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946431.1
    CDS206..3166
    Translation

    Target ORF information:

    RefSeq Version XM_018090942.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090942.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT
    ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA
    TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA
    AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG
    CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT
    GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT
    CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT
    GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC
    AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT
    ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG
    TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG
    GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG
    GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC
    GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA
    GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC
    ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG
    AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT
    GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA
    AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA
    TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA
    GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG
    ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT
    GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT
    TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA
    CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG
    GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA
    TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA
    CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA
    GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG
    ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT
    CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC
    TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT
    CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG
    CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA
    GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGCCAAT GAGTGAGGCC
    GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA
    CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA
    GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC
    GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC
    AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG
    GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG
    GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC
    GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA
    CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG
    CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG
    CCAAAGGCTG GCAAAAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41681
    Clone ID Related Accession (Same CDS sequence) XM_018090946.1
    Accession Version XM_018090946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGCCAAAA GAAGCAACAA CAGCATCTTC AGCAGCCCTG
    GCGGCTGCAT CCAAACCAAC AGCAGCACCT TCCTCAGCCC CAGTAACAGC AACCAAACCA
    ACAAAAACAA AGCCAAGTGC AGCAAAATCG CCTCCCAAAC AAGATATAAA GCCTATCCCT
    GGGAAGCAGC AAAAAACAGA CAGCAAGAGT AAAGATGAAA AGAAAAGTGG TGATAAAGTT
    TCTCCAGCTG TGCTACCTAA GATGACACCT AGTTCTGCTG GTAAAGTATC TGCAGTTGCC
    ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC GCTGCAGTCA CTGCTGTGCA GTCAACAAAG
    CCGAAAGATG AGAGTAAAGA GGAAAAGAAA GCCATTACTG GGGATAAGGT TTCTTCTGGG
    CCACCGGCCA AGACAGACGC AGATGCAGCC TTGGACGAAC TACTTGGAAC GTTAGAGGGC
    CCAGCAGATA TTCCAGAGTC TCCACAATTT ACTGGACCAG AAATCCAGGA CACAGTTGTT
    ACATCTGAGT ATTTGGAGGA GCTAGGAAAA CGGGATTTCA CCATCCCACC TGAATACAGG
    CACCTGCTGG ATGGTAAAGG TGAAAAGATG GCACCCCCTA CCCCACCCGA GGAACCCGAA
    GAGCACATGG ATGATGATGA ATTAGTTGCA GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG
    GCATCCCCCG AAGTAAAGAA GCCAAAGCTG GAAGAGAAAA TGGAAGTGAA ACCAAAATCA
    GCAGCAGCAG TGGCCACACA GCCAGATGTG CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA
    GAAACTTCCA AACCAAGCAC TCAGAAAAAA GAGGAAAAAG CAAAAATAGC AGAAGTGTCT
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA
    AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA
    CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA
    CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT
    ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA
    AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA
    CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT
    TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC
    GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC
    AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC
    GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA
    GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC
    AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT
    GGGAAAGTAG ATGGAAAAGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946435.1
    CDS206..3163
    Translation

    Target ORF information:

    RefSeq Version XM_018090946.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC
    AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA
    ACTATGAACC CGGCGGATAC ACAGCCAAAA GAAGCAACAA CAGCATCTTC AGCAGCCCTG
    GCGGCTGCAT CCAAACCAAC AGCAGCACCT TCCTCAGCCC CAGTAACAGC AACCAAACCA
    ACAAAAACAA AGCCAAGTGC AGCAAAATCG CCTCCCAAAC AAGATATAAA GCCTATCCCT
    GGGAAGCAGC AAAAAACAGA CAGCAAGAGT AAAGATGAAA AGAAAAGTGG TGATAAAGTT
    TCTCCAGCTG TGCTACCTAA GATGACACCT AGTTCTGCTG GTAAAGTATC TGCAGTTGCC
    ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC GCTGCAGTCA CTGCTGTGCA GTCAACAAAG
    CCGAAAGATG AGAGTAAAGA GGAAAAGAAA GCCATTACTG GGGATAAGGT TTCTTCTGGG
    CCACCGGCCA AGACAGACGC AGATGCAGCC TTGGACGAAC TACTTGGAAC GTTAGAGGGC
    CCAGCAGATA TTCCAGAGTC TCCACAATTT ACTGGACCAG AAATCCAGGA CACAGTTGTT
    ACATCTGAGT ATTTGGAGGA GCTAGGAAAA CGGGATTTCA CCATCCCACC TGAATACAGG
    CACCTGCTGG ATGGTAAAGG TGAAAAGATG GCACCCCCTA CCCCACCCGA GGAACCCGAA
    GAGCACATGG ATGATGATGA ATTAGTTGCA GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG
    GCATCCCCCG AAGTAAAGAA GCCAAAGCTG GAAGAGAAAA TGGAAGTGAA ACCAAAATCA
    GCAGCAGCAG TGGCCACACA GCCAGATGTG CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA
    GAAACTTCCA AACCAAGCAC TCAGAAAAAA GAGGAAAAAG CAAAAATAGC AGAAGTGTCT
    AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA
    AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA
    CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA
    CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT
    ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA
    AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA
    CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT
    TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC
    GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC
    AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC
    GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA
    GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC
    AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT
    GGGAAAGTAG ATGGAAAAGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT
    GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA
    CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA
    GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT
    CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT
    GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG
    CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA
    GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG
    CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG
    CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT
    TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT
    GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA
    CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC
    AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA
    CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT
    GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA
    CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG
    CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA
    AAGGCTGGCA AAAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41682
    Clone ID Related Accession (Same CDS sequence) XM_018090966.1
    Accession Version XM_018090966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2904bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG CCAAAAGAAG CAACAACAGC ATCTTCAGCA
    GCCCTGGCGG CTGCATCCAA ACCAACAGCA GCACCTTCCT CAGCCCCAGT AACAGCAACC
    AAACCAACAA AAACAAAGCC AAGTGCAGCA AAATCGCCTC CCAAACAAGA TATAAAGCCT
    ATCCCTGGGA AGCAGCAAAA AACAGACAGC AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT
    AAAGTTTCTC CAGCTGTGCT ACCTAAGATG ACACCTAGTT CTGCTGGTAA AGTATCTGCA
    GTTGCCACTG GAGCTCTAGC AGCTGCTGGT GCCGCCGCTG CAGTCACTGC TGTGCAGTCA
    ACAAAGCCGA AAGATGAGAG TAAAGAGGAA AAGAAAGCCA TTACTGGGGA TAAGGTTTCT
    TCTGGGCCAC CGGCCAAGAC AGACGCAGAT GCAGCCTTGG ACGAACTACT TGGAACGTTA
    GAGGGCCCAG CAGATATTCC AGAGTCTCCA CAATTTACTG GACCAGAAAT CCAGGACACA
    GTTGTTACAT CTGAGTATTT GGAGGAGCTA GGAAAACGGG ATTTCACCAT CCCACCTGAA
    TACAGGCACC TGCTGGATGG TAAAGGTGAA AAGATGGCAC CCCCTACCCC ACCCGAGGAA
    CCCGAAGAGC ACATGGATGA TGATGAATTA GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC
    AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA AAGCTGGAAG AGAAAATGGA AGTGAAACCA
    AAATCAGCAG CAGCAGTGGC CACACAGCCA GATGTGCAGA AAAAAGAAGT AAAAGTCGCA
    GCAGCAGAAA CTTCCAAACC AAGCACTCAG AAAAAAGAGG AAAAAGCAAA AATAGCAGAA
    GTGTCTAAAC CAAGCACTCA GAAAAAAGAG GAAAAACTGG CAGCAGCAGC AGACACATCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT
    CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA
    GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG
    CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT
    CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG
    GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA
    CTAGATGGGA AAGTAGATGG AAAAGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG
    ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA
    ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA
    GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA
    GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA
    CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA
    GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG
    GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG
    GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC
    CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT
    AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC
    GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA
    AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG
    GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT
    AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT
    GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT
    GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG
    TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT
    GTGCCAAAGG CTGGCAAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946455.1
    CDS206..3109
    Translation

    Target ORF information:

    RefSeq Version XM_018090966.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
    TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA
    GGAAAAACTA TGAACCCGGC GGATACACAG CCAAAAGAAG CAACAACAGC ATCTTCAGCA
    GCCCTGGCGG CTGCATCCAA ACCAACAGCA GCACCTTCCT CAGCCCCAGT AACAGCAACC
    AAACCAACAA AAACAAAGCC AAGTGCAGCA AAATCGCCTC CCAAACAAGA TATAAAGCCT
    ATCCCTGGGA AGCAGCAAAA AACAGACAGC AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT
    AAAGTTTCTC CAGCTGTGCT ACCTAAGATG ACACCTAGTT CTGCTGGTAA AGTATCTGCA
    GTTGCCACTG GAGCTCTAGC AGCTGCTGGT GCCGCCGCTG CAGTCACTGC TGTGCAGTCA
    ACAAAGCCGA AAGATGAGAG TAAAGAGGAA AAGAAAGCCA TTACTGGGGA TAAGGTTTCT
    TCTGGGCCAC CGGCCAAGAC AGACGCAGAT GCAGCCTTGG ACGAACTACT TGGAACGTTA
    GAGGGCCCAG CAGATATTCC AGAGTCTCCA CAATTTACTG GACCAGAAAT CCAGGACACA
    GTTGTTACAT CTGAGTATTT GGAGGAGCTA GGAAAACGGG ATTTCACCAT CCCACCTGAA
    TACAGGCACC TGCTGGATGG TAAAGGTGAA AAGATGGCAC CCCCTACCCC ACCCGAGGAA
    CCCGAAGAGC ACATGGATGA TGATGAATTA GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC
    AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA AAGCTGGAAG AGAAAATGGA AGTGAAACCA
    AAATCAGCAG CAGCAGTGGC CACACAGCCA GATGTGCAGA AAAAAGAAGT AAAAGTCGCA
    GCAGCAGAAA CTTCCAAACC AAGCACTCAG AAAAAAGAGG AAAAAGCAAA AATAGCAGAA
    GTGTCTAAAC CAAGCACTCA GAAAAAAGAG GAAAAACTGG CAGCAGCAGC AGACACATCC
    AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT
    TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT
    GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG
    AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA
    TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA
    CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT
    TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT
    CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA
    GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG
    CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT
    CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG
    GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA
    CTAGATGGGA AAGTAGATGG AAAAGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG
    ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA
    ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA
    GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA
    GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA
    CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA
    GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG
    GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG
    GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC
    CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT
    AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC
    GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA
    AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG
    GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT
    AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT
    GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT
    GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG
    TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT
    GTGCCAAAGG CTGGCAAAAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa41683
    Clone ID Related Accession (Same CDS sequence) XM_018090967.1
    Accession Version XM_018090967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calpastatin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
    CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGCCAAAAGA AGCAACAACA
    GCATCTTCAG CAGCCCTGGC GGCTGCATCC AAACCAACAG CAGCACCTTC CTCAGCCCCA
    GTAACAGCAA CCAAACCAAC AAAAACAAAG CCAAGTGCAG CAAAATCGCC TCCCAAACAA
    GATATAAAGC CTATCCCTGG GAAGCAGCAA AAAACAGACA GCAAGAGTAA AGATGAAAAG
    AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG CTACCTAAGA TGACACCTAG TTCTGCTGGT
    AAAGTATCTG CAGTTGCCAC TGGAGCTCTA GCAGCTGCTG GTGCCGCCGC TGCAGTCACT
    GCTGTGCAGT CAACAAAGCC GAAAGATGAG AGTAAAGAGG AAAAGAAAGC CATTACTGGG
    GATAAGGTTT CTTCTGGGCC ACCGGCCAAG ACAGACGCAG ATGCAGCCTT GGACGAACTA
    CTTGGAACGT TAGAGGGCCC AGCAGATATT CCAGAGTCTC CACAATTTAC TGGACCAGAA
    ATCCAGGACA CAGTTGTTAC ATCTGAGTAT TTGGAGGAGC TAGGAAAACG GGATTTCACC
    ATCCCACCTG AATACAGGCA CCTGCTGGAT GGTAAAGGTG AAAAGATGGC ACCCCCTACC
    CCACCCGAGG AACCCGAAGA GCACATGGAT GATGATGAAT TAGTTGCAGC CCTGTCTTCT
    GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA GTAAAGAAGC CAAAGCTGGA AGAGAAAATG
    GAAGTGAAAC CAAAATCAGC AGCAGCAGTG GCCACACAGC CAGATGTGCA GAAAAAAGAA
    GTAAAAGTCG CAGCAGCAGA AACTTCCAAA CCAAGCACTC AGAAAAAAGA GGAAAAAGCA
    AAAATAGCAG AAGTGTCTAA ACCAAGCACT CAGAAAAAAG AGGAAAAACT GGCAGCAGCA
    GCAGACACAT CCAAACCAAG CACTCAGAAA AAAGAGGTAA AAGCCACAGC AAAAGCCACA
    GCTGCCGAAG CTTCCAAACA AAGCACTGAG TCCATACCAT CAGAAGCAAT AGACGAGTTG
    TTGGGAACAC TTGAAGGACC CCCAGAAAAT GTTCCAGGGT CTCCAGTATT TCAGGGTCCA
    GAAGTTACTG AGAACATTAC AGCAACATAC TTAGAAGAAA CTGGAAAAAG AGATAGTACG
    ATCCCTCCAG AATACAGAAA ATTATTAGAT GGAAAGGATC ATGGGAAAGA TGTGTGGCCT
    TCAGCACCTG AACCAGAACC GTCTATGACC GACACAGAGC TTGTAGATGT GTTTTCCAAA
    GACTTTGAGC TTTCATGTTC ACCTACTGTT CAGACCACGC CAGCCATTAA ACCCAAAAGG
    AACACTGAAG ATCTCCACGA GTGCTATTGC TCAAAGTCAG TGAGCCAACT TTCATCATCT
    GAAACTCCTC AAGGTAACAA AACGAAACCT GAAATTGCGG CATCATCTGC ATCCACTGTA
    AAAGCAGCAG CGCCCTCCGG TGGTATGGAC AGTGCATTAG ATGAATTATT GGGAACACTG
    GAAGGTCCTG ATCCAACAGC CCCTGAATCA CCTGTGTACA CTGGGCCAGA GGTGACGGAA
    ACAGAGACAG CGGTATACAG AGAGGGACTT GGGATAAGAG ACAGCAGCAT CCCTCCAGCA
    TACAGGCACT TACTAGATGG GAAAGTAGAT GGAAAAGATG CACCCCCACC ACCGCCAGAG
    GAAAAGCCTC TGACCGATGA TCAGCTGATT GATAAGTTTT CTATGGACTT TGATTGTTCT
    GAATCTCCTG CAACACAACA GGCTCATCCT GTCGAACCTA AAGTTTCCTC GCAAGAGAAA
    AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC TCTGCTACTG TTAAAGCTGC ACCCACTCCT
    TCTAAAGCAC CAGCACCTCC AGCTGATCCA CTCGATAGCC TGTCTGGTAC CTTAGGTGTT
    CGAAAGGAAG AACCGAGTGA CAAGAAGCCT CCAGTGGATA AAGTAAAGGA GGGAACAGGC
    AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT GAAAAAACAA TACATCCTGA CCGCCGGCTA
    AAGGAAGTGA AGGATAAAGA CGGAAAGCCC TTGCTGCCAA AACTTGAACA AAGCCCTAAG
    CCAATGAGTG AGGCCGAGCT GCTGGATGCT TTGACTGAAG GCTTTGTGAC TTCCCCTGCC
    ACTCCTGATG CCCCACTGCA GGGCTCTTTG AAAAAAAAAG GTTCTGGATC AGAAGATGTT
    ATATCTTGTT CTAAAGCTTC CGCCGTCAAG TCTGGAGCTC CACAGGACGC TGCTTTAGAT
    ATACAGATTC CCGACGATGC ACTAGATTTA CTTTCTGGCT CCCTGGGACA AAGAGAGGTG
    GATCCTAATG AAAACAAACC GGTGGTGGAT GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT
    ATAGACAAAC TGGGGGACAG AGATGATACA ATCCCACCGG AATACAGAAA GCTGCTGGAT
    GGTAAAGATG ATAAGGGACA ACCTGCAAAA CCACCAGCGA AAGCGGAGGA GAAACCAAAG
    AAGCCTTTAA GTGACGATGC AGCGATCGAT GCTCTTTCAA GCGGATTTGC ATCATGTGAC
    ACAAAATCTA CTGAACAACC AAAGCAGCCT TCAACGGACA AGACTGAGAA ACAATGTGCT
    AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG GCACCTGATG CTGCAAAGCA GGCAAACTCA
    GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946456.1
    CDS206..3061
    Translation

    Target ORF information:

    RefSeq Version XM_018090967.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
    CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGCCAAAAGA AGCAACAACA
    GCATCTTCAG CAGCCCTGGC GGCTGCATCC AAACCAACAG CAGCACCTTC CTCAGCCCCA
    GTAACAGCAA CCAAACCAAC AAAAACAAAG CCAAGTGCAG CAAAATCGCC TCCCAAACAA
    GATATAAAGC CTATCCCTGG GAAGCAGCAA AAAACAGACA GCAAGAGTAA AGATGAAAAG
    AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG CTACCTAAGA TGACACCTAG TTCTGCTGGT
    AAAGTATCTG CAGTTGCCAC TGGAGCTCTA GCAGCTGCTG GTGCCGCCGC TGCAGTCACT
    GCTGTGCAGT CAACAAAGCC GAAAGATGAG AGTAAAGAGG AAAAGAAAGC CATTACTGGG
    GATAAGGTTT CTTCTGGGCC ACCGGCCAAG ACAGACGCAG ATGCAGCCTT GGACGAACTA
    CTTGGAACGT TAGAGGGCCC AGCAGATATT CCAGAGTCTC CACAATTTAC TGGACCAGAA
    ATCCAGGACA CAGTTGTTAC ATCTGAGTAT TTGGAGGAGC TAGGAAAACG GGATTTCACC
    ATCCCACCTG AATACAGGCA CCTGCTGGAT GGTAAAGGTG AAAAGATGGC ACCCCCTACC
    CCACCCGAGG AACCCGAAGA GCACATGGAT GATGATGAAT TAGTTGCAGC CCTGTCTTCT
    GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA GTAAAGAAGC CAAAGCTGGA AGAGAAAATG
    GAAGTGAAAC CAAAATCAGC AGCAGCAGTG GCCACACAGC CAGATGTGCA GAAAAAAGAA
    GTAAAAGTCG CAGCAGCAGA AACTTCCAAA CCAAGCACTC AGAAAAAAGA GGAAAAAGCA
    AAAATAGCAG AAGTGTCTAA ACCAAGCACT CAGAAAAAAG AGGAAAAACT GGCAGCAGCA
    GCAGACACAT CCAAACCAAG CACTCAGAAA AAAGAGGTAA AAGCCACAGC AAAAGCCACA
    GCTGCCGAAG CTTCCAAACA AAGCACTGAG TCCATACCAT CAGAAGCAAT AGACGAGTTG
    TTGGGAACAC TTGAAGGACC CCCAGAAAAT GTTCCAGGGT CTCCAGTATT TCAGGGTCCA
    GAAGTTACTG AGAACATTAC AGCAACATAC TTAGAAGAAA CTGGAAAAAG AGATAGTACG
    ATCCCTCCAG AATACAGAAA ATTATTAGAT GGAAAGGATC ATGGGAAAGA TGTGTGGCCT
    TCAGCACCTG AACCAGAACC GTCTATGACC GACACAGAGC TTGTAGATGT GTTTTCCAAA
    GACTTTGAGC TTTCATGTTC ACCTACTGTT CAGACCACGC CAGCCATTAA ACCCAAAAGG
    AACACTGAAG ATCTCCACGA GTGCTATTGC TCAAAGTCAG TGAGCCAACT TTCATCATCT
    GAAACTCCTC AAGGTAACAA AACGAAACCT GAAATTGCGG CATCATCTGC ATCCACTGTA
    AAAGCAGCAG CGCCCTCCGG TGGTATGGAC AGTGCATTAG ATGAATTATT GGGAACACTG
    GAAGGTCCTG ATCCAACAGC CCCTGAATCA CCTGTGTACA CTGGGCCAGA GGTGACGGAA
    ACAGAGACAG CGGTATACAG AGAGGGACTT GGGATAAGAG ACAGCAGCAT CCCTCCAGCA
    TACAGGCACT TACTAGATGG GAAAGTAGAT GGAAAAGATG CACCCCCACC ACCGCCAGAG
    GAAAAGCCTC TGACCGATGA TCAGCTGATT GATAAGTTTT CTATGGACTT TGATTGTTCT
    GAATCTCCTG CAACACAACA GGCTCATCCT GTCGAACCTA AAGTTTCCTC GCAAGAGAAA
    AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC TCTGCTACTG TTAAAGCTGC ACCCACTCCT
    TCTAAAGCAC CAGCACCTCC AGCTGATCCA CTCGATAGCC TGTCTGGTAC CTTAGGTGTT
    CGAAAGGAAG AACCGAGTGA CAAGAAGCCT CCAGTGGATA AAGTAAAGGA GGGAACAGGC
    AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT GAAAAAACAA TACATCCTGA CCGCCGGCTA
    AAGGAAGTGA AGGATAAAGA CGGAAAGCCC TTGCTGCCAA AACTTGAACA AAGCCCTAAG
    CCAATGAGTG AGGCCGAGCT GCTGGATGCT TTGACTGAAG GCTTTGTGAC TTCCCCTGCC
    ACTCCTGATG CCCCACTGCA GGGCTCTTTG AAAAAAAAAG GTTCTGGATC AGAAGATGTT
    ATATCTTGTT CTAAAGCTTC CGCCGTCAAG TCTGGAGCTC CACAGGACGC TGCTTTAGAT
    ATACAGATTC CCGACGATGC ACTAGATTTA CTTTCTGGCT CCCTGGGACA AAGAGAGGTG
    GATCCTAATG AAAACAAACC GGTGGTGGAT GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT
    ATAGACAAAC TGGGGGACAG AGATGATACA ATCCCACCGG AATACAGAAA GCTGCTGGAT
    GGTAAAGATG ATAAGGGACA ACCTGCAAAA CCACCAGCGA AAGCGGAGGA GAAACCAAAG
    AAGCCTTTAA GTGACGATGC AGCGATCGAT GCTCTTTCAA GCGGATTTGC ATCATGTGAC
    ACAAAATCTA CTGAACAACC AAAGCAGCCT TCAACGGACA AGACTGAGAA ACAATGTGCT
    AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG GCACCTGATG CTGCAAAGCA GGCAAACTCA
    GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.