DYSF cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	DYSF
Entrez Gene ID	459315
Full Name	dysferlin
General protein information	Preferred Names dysferlin Names dysferlin dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016948730.1	XP_016804219.1	dysferlin isoform X2
XM_016948731.1	XP_016804220.1	dysferlin isoform X3
XM_016948732.1	XP_016804221.1	dysferlin isoform X4
XM_016948735.1	XP_016804224.1	dysferlin isoform X7
XM_016948736.1	XP_016804225.1	dysferlin isoform X8
XM_016948738.1	XP_016804227.1	dysferlin isoform X10
XM_016948740.1	XP_016804229.1	dysferlin isoform X12
XM_016948748.1	XP_016804237.1	dysferlin isoform X20
XM_016948750.1	XP_016804239.1	dysferlin isoform X22
XM_016948754.1	XP_016804243.1	dysferlin isoform X26
XM_016948734.2	XP_016804223.1	dysferlin isoform X6
XM_016948737.2	XP_016804226.1	dysferlin isoform X9
XM_016948738.2	XP_016804227.1	dysferlin isoform X10
XM_016948739.2	XP_016804228.1	dysferlin isoform X11
XM_016948750.2	XP_016804239.1	dysferlin isoform X23
XM_016948745.1	XP_016804234.1	dysferlin isoform X17
XM_016948747.1	XP_016804236.1	dysferlin isoform X19
XM_016948752.1	XP_016804241.1	dysferlin isoform X24
XM_016948733.2	XP_016804222.1	dysferlin isoform X5
XM_016948743.2	XP_016804232.1	dysferlin isoform X15
XM_016948745.2	XP_016804234.1	dysferlin isoform X17
XM_016948729.1	XP_016804218.1	dysferlin isoform X1
XM_016948734.1	XP_016804223.1	dysferlin isoform X6
XM_016948739.1	XP_016804228.1	dysferlin isoform X11
XM_016948741.1	XP_016804230.1	dysferlin isoform X13
XM_016948742.1	XP_016804231.1	dysferlin isoform X14
XM_016948743.1	XP_016804232.1	dysferlin isoform X15
XM_016948744.1	XP_016804233.1	dysferlin isoform X16
XM_016948753.1	XP_016804242.1	dysferlin isoform X25
XM_016948730.2	XP_016804219.1	dysferlin isoform X2
XM_016948731.2	XP_016804220.1	dysferlin isoform X3
XM_016948736.2	XP_016804225.1	dysferlin isoform X8
XM_016948740.2	XP_016804229.1	dysferlin isoform X12
XM_016948744.2	XP_016804233.1	dysferlin isoform X16
XM_016948748.2	XP_016804237.1	dysferlin isoform X20
XM_016948749.2	XP_016804238.1	dysferlin isoform X21
XM_024354432.1	XP_024210200.1	dysferlin isoform X22
XM_016948737.1	XP_016804226.1	dysferlin isoform X9
XM_016948729.2	XP_016804218.1	dysferlin isoform X1
XM_016948741.2	XP_016804230.1	dysferlin isoform X13
XM_016948746.2	XP_016804235.1	dysferlin isoform X18
XM_016948751.2	XP_016804240.1	dysferlin isoform X24
XM_016948752.2	XP_016804241.1	dysferlin isoform X25
XM_016948733.1	XP_016804222.1	dysferlin isoform X5
XM_016948746.1	XP_016804235.1	dysferlin isoform X18
XM_016948749.1	XP_016804238.1	dysferlin isoform X21
XM_016948751.1	XP_016804240.1	dysferlin isoform X23
XM_016948732.2	XP_016804221.1	dysferlin isoform X4
XM_016948735.2	XP_016804224.1	dysferlin isoform X7
XM_016948742.2	XP_016804231.1	dysferlin isoform X14
XM_016948747.2	XP_016804236.1	dysferlin isoform X19
XM_016948753.2	XP_016804242.1	dysferlin isoform X26
XM_016948754.2	XP_016804243.1	dysferlin isoform X27

Pathways from BioSystems

Homologies

Homo sapiens (human)	DYSF	NP_001124454.1
	DYSF	XP_002799336.1
Caenorhabditis elegans (roundworm)	fer-1	NP_492337.1
Caenorhabditis elegans (roundworm)	ferl-1	NP_491602.2
Rattus norvegicus (Norway rat)	Dysf	NP_001101339.1
Danio rerio (zebrafish)	dysf	NP_001244122.1
Mus musculus (house mouse)	Dysf	NP_067444.2
Canis lupus familiaris (dog)	DYSF	XP_003432278.1
Bos taurus (cattle)	DYSF	NP_001095960.1
Pan troglodytes (chimpanzee)	DYSF	XP_003309136.1
Gallus gallus (chicken)	DYSF	XP_423126.4

Gene Ontology

The following DYSF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DYSF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc103416	XM_016948729.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103417	XM_016948730.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103418	XM_016948731.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103419	XM_016948732.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103420	XM_016948733.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103421	XM_016948734.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103422	XM_016948735.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103423	XM_016948736.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103424	XM_016948737.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103425	XM_016948738.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103426	XM_016948739.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103427	XM_016948740.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103428	XM_016948741.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103429	XM_016948742.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103430	XM_016948743.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103431	XM_016948744.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103432	XM_016948745.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103433	XM_016948746.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103434	XM_016948747.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103435	XM_016948748.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103436	XM_016948749.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103437	XM_024354432.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103438	XM_016948750.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103439	XM_016948751.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103440	XM_016948752.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103441	XM_016948753.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc103442	XM_016948754.2 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPc79268	XM_016948729.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79269	XM_016948730.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79270	XM_016948731.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79271	XM_016948732.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79272	XM_016948733.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79273	XM_016948734.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79274	XM_016948735.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79275	XM_016948736.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79276	XM_016948737.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79277	XM_016948738.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79278	XM_016948739.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79279	XM_016948740.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79280	XM_016948741.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79281	XM_016948742.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79282	XM_016948743.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79283	XM_016948744.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79284	XM_016948745.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79285	XM_016948746.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79286	XM_016948747.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79287	XM_016948748.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79288	XM_016948749.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79289	XM_016948750.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79290	XM_016948751.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79291	XM_016948752.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79292	XM_016948753.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OPc79293	XM_016948754.1 Latest version!	Pan troglodytes dysferlin (DYSF), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DYSF ( XM_016948729.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804218.1 Pan troglodytes dysferlin (DYSF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc103416
Clone ID Related Accession (Same CDS sequence)	XM_016948729.2
Accession Version	XM_016948729.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TAAGACCCTC 
CGGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCATCGACCT GGAAAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804218.1
CDS	157..6612
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948729.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948729.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TAAGACCCTC 
CGGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCATCGACCT GGAAAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948730.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804219.1 Pan troglodytes dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OPc103417
Clone ID Related Accession (Same CDS sequence)	XM_016948730.2
Accession Version	XM_016948730.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG AGACATCAAG GATCCTGGAT GAGCTGTGTG CTCCTGCCTT CTTCCTGCAG 
GGCCTTCTCC AAACGTCTGA GGACACAGAC CTGCCCTACC CACCACCCCA GAGGGAGGCC 
AACATCTACA TGGTTCCTCA GAACATCAAG CCAGCGCTCC AGCGTACCGC CATTGAGATC 
CTGGCATGGG GCCTGCGGAA CATGAAGAGT TACCAGCTGG CCAACATCTC CTCCCCCAGC 
CTCGTGGTAG AGTGTCGGGG CCAGACGGTG CAGTCCTGTG TCATCAGGAA CCTCCGGAAG 
AACCCCAACT TTGACATCTG CACCCTCTTC ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC 
TACTGCCCCC CCATCACCGT CAAGGTCATC GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG CTCCCTGGAG AGCTTCCTGT GTGACCCCTA CTCGGCGGAG 
AGTCCATCCC CACAGGGTGG CCCAGACGAT GTGAGCCTAC TCAGTCCTGG GGAAGACGTG 
CTCATCGACA TTGATGACAA GGAGCCCCTC ATCCCCATCC AGCTTGCAGA CGGTCTGTCG 
AGCTTGGCCC CCACTAACAC GGCTTCTCCT CCATCCAGTC CTCATAAGAC CCTCCGGGAG 
GAAGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC 
GGCTCCTACT TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTGTA CCGGGGCAAG 
ACGCAGGAGG AGACAGAAGA TCCATCTGTC ATTGGTGAAT TTAAGGGCCT CTTCAAAATT 
TATCCCCTCC CAGAAGACCC AGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTGGCC 
GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT ATCTACATTG TCCGAGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCCTGC ACGCTGGAGC CCGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTAAAGA TCACTCTCTA TGACTATGAC 
CTCCTCTCCA AGGACGAAAA GATCGGTGAG ACGGTCATCG ACCTGGAAAA CAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG 
TGGCGGGACC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCATAGAGTC 
AAGGCACCTG TGTACCGGAC AGACCGTGTA ATGTTTCAGG ATAAAGAATA TTCCATTGAA 
GAGATAGAGG CTGGCAGGAT CCCAAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCT 
CTGCATGTGC TTCAGCAGCA GGGCCTGGTC CCGGAGCACG TGGAGTCACG GCCCCTCTAC 
AGCCCCCTGC AGCCAGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTATTTCCG 
AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAATACCAGA GATGTGATCC TGGATGACCT GAGCCTCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGA TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ACGTGCATTA TCGTTCCCTG GGAGGTGAAG GCAACTTCAA CTGGAGGTTC 
ATTTTCCCCT TCGACTACCT GCCAGCTGAG CAAGTCTGTA CCATTGCCAA GAAGGATGCC 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTGTT CCAGATCTGG 
GACAATGACA AGTTCTCCTT TGATGATTTT CTGGGCTCCC TGCAGCTCGA TCTCAACCGC 
ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG TGCTCCTTGG ACCAGCTGGA TGATGCTTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTAGCAGAAG AGGGTGAGAA GAAAATACTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA GCCCAACATG 
AACCCTAAGC TTGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT TACCTCCCCA 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GGGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC ATCTTCATCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804219.1
CDS	157..6606
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948730.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948730.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG AGACATCAAG GATCCTGGAT GAGCTGTGTG CTCCTGCCTT CTTCCTGCAG 
GGCCTTCTCC AAACGTCTGA GGACACAGAC CTGCCCTACC CACCACCCCA GAGGGAGGCC 
AACATCTACA TGGTTCCTCA GAACATCAAG CCAGCGCTCC AGCGTACCGC CATTGAGATC 
CTGGCATGGG GCCTGCGGAA CATGAAGAGT TACCAGCTGG CCAACATCTC CTCCCCCAGC 
CTCGTGGTAG AGTGTCGGGG CCAGACGGTG CAGTCCTGTG TCATCAGGAA CCTCCGGAAG 
AACCCCAACT TTGACATCTG CACCCTCTTC ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC 
TACTGCCCCC CCATCACCGT CAAGGTCATC GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG CTCCCTGGAG AGCTTCCTGT GTGACCCCTA CTCGGCGGAG 
AGTCCATCCC CACAGGGTGG CCCAGACGAT GTGAGCCTAC TCAGTCCTGG GGAAGACGTG 
CTCATCGACA TTGATGACAA GGAGCCCCTC ATCCCCATCC AGCTTGCAGA CGGTCTGTCG 
AGCTTGGCCC CCACTAACAC GGCTTCTCCT CCATCCAGTC CTCATAAGAC CCTCCGGGAG 
GAAGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC 
GGCTCCTACT TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTGTA CCGGGGCAAG 
ACGCAGGAGG AGACAGAAGA TCCATCTGTC ATTGGTGAAT TTAAGGGCCT CTTCAAAATT 
TATCCCCTCC CAGAAGACCC AGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTGGCC 
GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT ATCTACATTG TCCGAGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCCTGC ACGCTGGAGC CCGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTAAAGA TCACTCTCTA TGACTATGAC 
CTCCTCTCCA AGGACGAAAA GATCGGTGAG ACGGTCATCG ACCTGGAAAA CAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG 
TGGCGGGACC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCATAGAGTC 
AAGGCACCTG TGTACCGGAC AGACCGTGTA ATGTTTCAGG ATAAAGAATA TTCCATTGAA 
GAGATAGAGG CTGGCAGGAT CCCAAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCT 
CTGCATGTGC TTCAGCAGCA GGGCCTGGTC CCGGAGCACG TGGAGTCACG GCCCCTCTAC 
AGCCCCCTGC AGCCAGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTATTTCCG 
AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAATACCAGA GATGTGATCC TGGATGACCT GAGCCTCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGA TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ACGTGCATTA TCGTTCCCTG GGAGGTGAAG GCAACTTCAA CTGGAGGTTC 
ATTTTCCCCT TCGACTACCT GCCAGCTGAG CAAGTCTGTA CCATTGCCAA GAAGGATGCC 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTGTT CCAGATCTGG 
GACAATGACA AGTTCTCCTT TGATGATTTT CTGGGCTCCC TGCAGCTCGA TCTCAACCGC 
ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG TGCTCCTTGG ACCAGCTGGA TGATGCTTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTAGCAGAAG AGGGTGAGAA GAAAATACTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA GCCCAACATG 
AACCCTAAGC TTGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT TACCTCCCCA 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GGGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC ATCTTCATCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948731.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804220.1 Pan troglodytes dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OPc103418
Clone ID Related Accession (Same CDS sequence)	XM_016948731.2
Accession Version	XM_016948731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804220.1
CDS	156..6599
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948731.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948731.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948732.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804221.1 Pan troglodytes dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OPc103419
Clone ID Related Accession (Same CDS sequence)	XM_016948732.2
Accession Version	XM_016948732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATA AGACCCTCCG GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804221.1
CDS	155..6568
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948732.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948732.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATA AGACCCTCCG GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948733.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804222.1 Pan troglodytes dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OPc103420
Clone ID Related Accession (Same CDS sequence)	XM_016948733.2
Accession Version	XM_016948733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGCTG 
TGTGCTCCTG CCTTCTTCCT GCAGGGCCTT CTCCAAACGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATA AGACCCTCCG GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804222.1
CDS	156..6569
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948733.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948733.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGCTG 
TGTGCTCCTG CCTTCTTCCT GCAGGGCCTT CTCCAAACGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATA AGACCCTCCG GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948734.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804223.1 Pan troglodytes dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OPc103421
Clone ID Related Accession (Same CDS sequence)	XM_016948734.2
Accession Version	XM_016948734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG AGACATCAAG GATCCTGGAT GAGTCTGAGG ACACAGACCT GCCCTACCCA 
CCACCCCAGA GGGAGGCCAA CATCTACATG GTTCCTCAGA ACATCAAGCC AGCGCTCCAG 
CGTACCGCCA TTGAGATCCT GGCATGGGGC CTGCGGAACA TGAAGAGTTA CCAGCTGGCC 
AACATCTCCT CCCCCAGCCT CGTGGTAGAG TGTCGGGGCC AGACGGTGCA GTCCTGTGTC 
ATCAGGAACC TCCGGAAGAA CCCCAACTTT GACATCTGCA CCCTCTTCAT GGAAGTGATG 
CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC ATCACCGTCA AGGTCATCGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGCT CCCTGGAGAG CTTCCTGTGT 
GACCCCTACT CGGCGGAGAG TCCATCCCCA CAGGGTGGCC CAGACGATGT GAGCCTACTC 
AGTCCTGGGG AAGACGTGCT CATCGACATT GATGACAAGG AGCCCCTCAT CCCCATCCAG 
CTTGCAGACG GTCTGTCGAG CTTGGCCCCC ACTAACACGG CTTCTCCTCC ATCCAGTCCT 
CATAAGACCC TCCGGGAGGA AGAGTTCATC GATTGGTGGA GCAAATTCTT TGCCTCCATA 
GGGGAGAGGG AAAAGTGCGG CTCCTACTTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTGTACC GGGGCAAGAC GCAGGAGGAG ACAGAAGATC CATCTGTCAT TGGTGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCAG CCATCCCCAT GCCCCCAAGA 
CAGTTCCACC AGCTGGCCGC CCAGGGACCC CAGGAGTGCT TGGTCCGTAT CTACATTGTC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCCTGCAC GCTGGAGCCC 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTAAAGATC 
ACTCTCTATG ACTATGACCT CCTCTCCAAG GACGAAAAGA TCGGTGAGAC GGTCATCGAC 
CTGGAAAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CGAACCAGTG GCGGGACCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC ATAGAGTCAA GGCACCTGTG TACCGGACAG ACCGTGTAAT GTTTCAGGAT 
AAAGAATATT CCATTGAAGA GATAGAGGCT GGCAGGATCC CAAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCTCT GCATGTGCTT CAGCAGCAGG GCCTGGTCCC GGAGCACGTG 
GAGTCACGGC CCCTCTACAG CCCCCTGCAG CCAGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TATTTCCGAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ATACCAGAGA TGTGATCCTG 
GATGACCTGA GCCTCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGATT 
GGCTTTGAAG AACACAAGCA AAAGACAGAC GTGCATTATC GTTCCCTGGG AGGTGAAGGC 
AACTTCAACT GGAGGTTCAT TTTCCCCTTC GACTACCTGC CAGCTGAGCA AGTCTGTACC 
ATTGCCAAGA AGGATGCCTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGGTGTTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTTCT GGGCTCCCTG 
CAGCTCGATC TCAACCGCAT GCCCAAGCCA GCCAAGACAG CCGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ATGCTTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT AGCAGAAGAG GGTGAGAAGA AAATACTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATTGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC 
CGGGATGAGC CCAACATGAA CCCTAAGCTT GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTTA CCTCCCCATA CAAGACCATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGG 
GCCATCATCC TCTTCATCAT CCTCTTCATC CTGCTGCTGT TCCTGGCCAT CTTCATCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804223.1
CDS	156..6563
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948734.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948734.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG AGACATCAAG GATCCTGGAT GAGTCTGAGG ACACAGACCT GCCCTACCCA 
CCACCCCAGA GGGAGGCCAA CATCTACATG GTTCCTCAGA ACATCAAGCC AGCGCTCCAG 
CGTACCGCCA TTGAGATCCT GGCATGGGGC CTGCGGAACA TGAAGAGTTA CCAGCTGGCC 
AACATCTCCT CCCCCAGCCT CGTGGTAGAG TGTCGGGGCC AGACGGTGCA GTCCTGTGTC 
ATCAGGAACC TCCGGAAGAA CCCCAACTTT GACATCTGCA CCCTCTTCAT GGAAGTGATG 
CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC ATCACCGTCA AGGTCATCGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGCT CCCTGGAGAG CTTCCTGTGT 
GACCCCTACT CGGCGGAGAG TCCATCCCCA CAGGGTGGCC CAGACGATGT GAGCCTACTC 
AGTCCTGGGG AAGACGTGCT CATCGACATT GATGACAAGG AGCCCCTCAT CCCCATCCAG 
CTTGCAGACG GTCTGTCGAG CTTGGCCCCC ACTAACACGG CTTCTCCTCC ATCCAGTCCT 
CATAAGACCC TCCGGGAGGA AGAGTTCATC GATTGGTGGA GCAAATTCTT TGCCTCCATA 
GGGGAGAGGG AAAAGTGCGG CTCCTACTTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTGTACC GGGGCAAGAC GCAGGAGGAG ACAGAAGATC CATCTGTCAT TGGTGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCAG CCATCCCCAT GCCCCCAAGA 
CAGTTCCACC AGCTGGCCGC CCAGGGACCC CAGGAGTGCT TGGTCCGTAT CTACATTGTC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCCTGCAC GCTGGAGCCC 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTAAAGATC 
ACTCTCTATG ACTATGACCT CCTCTCCAAG GACGAAAAGA TCGGTGAGAC GGTCATCGAC 
CTGGAAAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CGAACCAGTG GCGGGACCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC ATAGAGTCAA GGCACCTGTG TACCGGACAG ACCGTGTAAT GTTTCAGGAT 
AAAGAATATT CCATTGAAGA GATAGAGGCT GGCAGGATCC CAAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCTCT GCATGTGCTT CAGCAGCAGG GCCTGGTCCC GGAGCACGTG 
GAGTCACGGC CCCTCTACAG CCCCCTGCAG CCAGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TATTTCCGAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ATACCAGAGA TGTGATCCTG 
GATGACCTGA GCCTCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGATT 
GGCTTTGAAG AACACAAGCA AAAGACAGAC GTGCATTATC GTTCCCTGGG AGGTGAAGGC 
AACTTCAACT GGAGGTTCAT TTTCCCCTTC GACTACCTGC CAGCTGAGCA AGTCTGTACC 
ATTGCCAAGA AGGATGCCTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGGTGTTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTTCT GGGCTCCCTG 
CAGCTCGATC TCAACCGCAT GCCCAAGCCA GCCAAGACAG CCGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ATGCTTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT AGCAGAAGAG GGTGAGAAGA AAATACTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATTGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC 
CGGGATGAGC CCAACATGAA CCCTAAGCTT GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTTA CCTCCCCATA CAAGACCATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGG 
GCCATCATCC TCTTCATCAT CCTCTTCATC CTGCTGCTGT TCCTGGCCAT CTTCATCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948735.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804224.1 Pan troglodytes dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OPc103422
Clone ID Related Accession (Same CDS sequence)	XM_016948735.2
Accession Version	XM_016948735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGTGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCCCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGCTGGA GCCCGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTAAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAGGACGAA AAGATCGGTG AGACGGTCAT CGACCTGGAA 
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCATAGAG TCAAGGCACC TGTGTACCGG ACAGACCGTG TAATGTTTCA GGATAAAGAA 
TATTCCATTG AAGAGATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCAGCAG CAGGGCCTGG TCCCGGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTATTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGTTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCAGCTG AGCAAGTCTG TACCATTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTG 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGATGCTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAAATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTTACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804224.1
CDS	155..6556
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948735.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948735.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGTGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCCCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGCTGGA GCCCGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTAAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAGGACGAA AAGATCGGTG AGACGGTCAT CGACCTGGAA 
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCATAGAG TCAAGGCACC TGTGTACCGG ACAGACCGTG TAATGTTTCA GGATAAAGAA 
TATTCCATTG AAGAGATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCAGCAG CAGGGCCTGG TCCCGGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTATTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGTTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCAGCTG AGCAAGTCTG TACCATTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTG 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGATGCTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAAATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTTACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948736.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804225.1 Pan troglodytes dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OPc103423
Clone ID Related Accession (Same CDS sequence)	XM_016948736.2
Accession Version	XM_016948736.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948736.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG GCCTTCTCCA AACGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATAAGACC 
CTCCGGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCATCGA CCTGGAAAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804225.1
CDS	156..6554
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948736.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948736.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG GCCTTCTCCA AACGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATAAGACC 
CTCCGGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCATCGA CCTGGAAAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948737.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804226.1 Pan troglodytes dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OPc103424
Clone ID Related Accession (Same CDS sequence)	XM_016948737.2
Accession Version	XM_016948737.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCATC GACCTGGAAA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804226.1
CDS	156..6536
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948737.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948737.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCATC GACCTGGAAA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948738.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804227.1 Pan troglodytes dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OPc103425
Clone ID Related Accession (Same CDS sequence)	XM_016948738.2
Accession Version	XM_016948738.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGCTGT GTGCTCCTGC CTTCTTCCTG CAGGGCCTTC TCCAAACGTC TGAGGACACA 
GACCTGCCCT ACCCACCACC CCAGAGGGAG GCCAACATCT ACATGGTTCC TCAGAACATC 
AAGCCAGCGC TCCAGCGTAC CGCCATTGAG ATCCTGGCAT GGGGCCTGCG GAACATGAAG 
AGTTACCAGC TGGCCAACAT CTCCTCCCCC AGCCTCGTGG TAGAGTGTCG GGGCCAGACG 
GTGCAGTCCT GTGTCATCAG GAACCTCCGG AAGAACCCCA ACTTTGACAT CTGCACCCTC 
TTCATGGAAG TGATGCTGCC CAGGGAGGAG CTCTACTGCC CCCCCATCAC CGTCAAGGTC 
ATCGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGCTCCCTG 
GAGAGCTTCC TGTGTGACCC CTACTCGGCG GAGAGTCCAT CCCCACAGGG TGGCCCAGAC 
GATGTGAGCC TACTCAGTCC TGGGGAAGAC GTGCTCATCG ACATTGATGA CAAGGAGCCC 
CTCATCCCCA TCCAGCTTGC AGACGGTCTG TCGAGCTTGG CCCCCACTAA CACGGCTTCT 
CCTCCATCCA GTCCTCATAA GACCCTCCGG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCA TCGACCTGGA AAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804227.1
CDS	156..6518
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948738.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948738.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGCTGT GTGCTCCTGC CTTCTTCCTG CAGGGCCTTC TCCAAACGTC TGAGGACACA 
GACCTGCCCT ACCCACCACC CCAGAGGGAG GCCAACATCT ACATGGTTCC TCAGAACATC 
AAGCCAGCGC TCCAGCGTAC CGCCATTGAG ATCCTGGCAT GGGGCCTGCG GAACATGAAG 
AGTTACCAGC TGGCCAACAT CTCCTCCCCC AGCCTCGTGG TAGAGTGTCG GGGCCAGACG 
GTGCAGTCCT GTGTCATCAG GAACCTCCGG AAGAACCCCA ACTTTGACAT CTGCACCCTC 
TTCATGGAAG TGATGCTGCC CAGGGAGGAG CTCTACTGCC CCCCCATCAC CGTCAAGGTC 
ATCGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGCTCCCTG 
GAGAGCTTCC TGTGTGACCC CTACTCGGCG GAGAGTCCAT CCCCACAGGG TGGCCCAGAC 
GATGTGAGCC TACTCAGTCC TGGGGAAGAC GTGCTCATCG ACATTGATGA CAAGGAGCCC 
CTCATCCCCA TCCAGCTTGC AGACGGTCTG TCGAGCTTGG CCCCCACTAA CACGGCTTCT 
CCTCCATCCA GTCCTCATAA GACCCTCCGG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCA TCGACCTGGA AAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948739.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804228.1 Pan troglodytes dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OPc103426
Clone ID Related Accession (Same CDS sequence)	XM_016948739.2
Accession Version	XM_016948739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTCGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCATCG ACCTGGAAAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804228.1
CDS	155..6514
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948739.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948739.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTCGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCATCG ACCTGGAAAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948740.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804229.1 Pan troglodytes dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OPc103427
Clone ID Related Accession (Same CDS sequence)	XM_016948740.2
Accession Version	XM_016948740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCATCGA CCTGGAAAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804229.1
CDS	155..6493
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948740.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948740.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCATCGA CCTGGAAAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948741.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804230.1 Pan troglodytes dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OPc103428
Clone ID Related Accession (Same CDS sequence)	XM_016948741.2
Accession Version	XM_016948741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTCGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG 
ATCGGTGAGA CGGTCATCGA CCTGGAAAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA 
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804230.1
CDS	155..6463
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948741.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948741.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTCGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG 
ATCGGTGAGA CGGTCATCGA CCTGGAAAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA 
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948742.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804231.1 Pan troglodytes dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OPc103429
Clone ID Related Accession (Same CDS sequence)	XM_016948742.2
Accession Version	XM_016948742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTCGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCATCGACC TGGAAAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804231.1
CDS	155..6451
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948742.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948742.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTCGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCATCGACC TGGAAAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948743.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804232.1 Pan troglodytes dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	OPc103430
Clone ID Related Accession (Same CDS sequence)	XM_016948743.2
Accession Version	XM_016948743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948743.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAT GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCGTC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TCCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACCA AGTTGGCCCT GGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGAGAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA AGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCC 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCATCGACC TGGAAAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG 
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804232.1
CDS	154..6420
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948743.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948743.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAT GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCGTC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TCCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACCA AGTTGGCCCT GGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGAGAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA AGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCC 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCATCGACC TGGAAAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG 
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948744.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804233.1 Pan troglodytes dysferlin (DYSF), transcript variant X16, mRNA.

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CloneID	OPc103431
Clone ID Related Accession (Same CDS sequence)	XM_016948744.2
Accession Version	XM_016948744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948744.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTCGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCATCGACCT GGAAAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804233.1
CDS	154..6399
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948744.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948744.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTCGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCATCGACCT GGAAAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948745.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804234.1 Pan troglodytes dysferlin (DYSF), transcript variant X17, mRNA.

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CloneID	OPc103432
Clone ID Related Accession (Same CDS sequence)	XM_016948745.2
Accession Version	XM_016948745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAT GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCGTC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TCCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACCA AGTTGGCCCT GGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGAGAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA AGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCC 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804234.1
CDS	154..6357
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948745.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948745.2

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAT GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCGTC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TCCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACCA AGTTGGCCCT GGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGAGAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA AGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCC 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948746.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804235.1 Pan troglodytes dysferlin (DYSF), transcript variant X18, mRNA.

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CloneID	OPc103433
Clone ID Related Accession (Same CDS sequence)	XM_016948746.2
Accession Version	XM_016948746.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ATGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCG TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC CAAGTTGGCC 
CTGGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGAGA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAAGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CCCGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC ATCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGCTGT GTGCTCCTGC CTTCTTCCTG 
CAGGGCCTTC TCCAAACGTC TGAGGACACA GACCTGCCCT ACCCACCACC CCAGAGGGAG 
GCCAACATCT ACATGGTTCC TCAGAACATC AAGCCAGCGC TCCAGCGTAC CGCCATTGAG 
ATCCTGGCAT GGGGCCTGCG GAACATGAAG AGTTACCAGC TGGCCAACAT CTCCTCCCCC 
AGCCTCGTGG TAGAGTGTCG GGGCCAGACG GTGCAGTCCT GTGTCATCAG GAACCTCCGG 
AAGAACCCCA ACTTTGACAT CTGCACCCTC TTCATGGAAG TGATGCTGCC CAGGGAGGAG 
CTCTACTGCC CCCCCATCAC CGTCAAGGTC ATCGATAACC GCCAGTTTGG CCGCCGGCCT 
GTGGTGGGCC AGTGTACCAT CCGCTCCCTG GAGAGCTTCC TGTGTGACCC CTACTCGGCG 
GAGAGTCCAT CCCCACAGGG TGGCCCAGAC GATGTGAGCC TACTCAGTCC TGGGGAAGAC 
GTGCTCATCG ACATTGATGA CAAGGAGCCC CTCATCCCCA TCCAGCTTGC AGACGGTCTG 
TCGAGCTTGG CCCCCACTAA CACGGCTTCT CCTCCATCCA GTCCTCATAA GACCCTCCGG 
GAGGAAGAGT TCATCGATTG GTGGAGCAAA TTCTTTGCCT CCATAGGGGA GAGGGAAAAG 
TGCGGCTCCT ACTTGGAGAA GGATTTTGAC ACCCTGAAGG TCTATGACAC ACAGCTGGAG 
AATGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT GTACCGGGGC 
AAGACGCAGG AGGAGACAGA AGATCCATCT GTCATTGGTG AATTTAAGGG CCTCTTCAAA 
ATTTATCCCC TCCCAGAAGA CCCAGCCATC CCCATGCCCC CAAGACAGTT CCACCAGCTG 
GCCGCCCAGG GACCCCAGGA GTGCTTGGTC CGTATCTACA TTGTCCGAGC ATTTGGCCTG 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATCCCC TGCACGCTGG AGCCCGTATT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTAA AGATCACTCT CTATGACTAT 
GACCTCCTCT CCAAGGACGA AAAGATCGGT GAGACGGTCA TCGACCTGGA AAACAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGTGGACTC CCACAGACCT ACTGTGTCTC TGGACCGAAC 
CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCATAGA 
GTCAAGGCAC CTGTGTACCG GACAGACCGT GTAATGTTTC AGGATAAAGA ATATTCCATT 
GAAGAGATAG AGGCTGGCAG GATCCCAAAC CCACACCTGG GCCCAGTGGA GGAGCGTCTG 
GCTCTGCATG TGCTTCAGCA GCAGGGCCTG GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC 
TACAGCCCCC TGCAGCCAGA CATCGAGCAG GGGAAGCTGC AGATGTGGGT CGACCTATTT 
CCGAAGGCCC TGGGGCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAATACC AGAGATGTGA TCCTGGATGA CCTGAGCCTC 
ACGGGGGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGATTGGCTT TGAAGAACAC 
AAGCAAAAGA CAGACGTGCA TTATCGTTCC CTGGGAGGTG AAGGCAACTT CAACTGGAGG 
TTCATTTTCC CCTTCGACTA CCTGCCAGCT GAGCAAGTCT GTACCATTGC CAAGAAGGAT 
GCCTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT GTTCCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGATGAT TTTCTGGGCT CCCTGCAGCT CGATCTCAAC 
CGCATGCCCA AGCCAGCCAA GACAGCCGAG AAGTGCTCCT TGGACCAGCT GGATGATGCT 
TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC 
TGTGTAGCAG AAGAGGGTGA GAAGAAAATA CTGGCGGGCA AGCTGGAAAT GACCTTGGAG 
ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC 
ATGAACCCTA AGCTTGAGGA CCCAAGGCGC CCCGACACCT CCTTCCTGTG GTTTACCTCC 
CCATACAAGA CCATGAAGTT CATCCTGTGG CGGCGTTTCC GGTGGGCCAT CATCCTCTTC 
ATCATCCTCT TCATCCTGCT GCTGTTCCTG GCCATCTTCA TCTACGCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804235.1
CDS	891..7343
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948746.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948746.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ATGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCG TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC CAAGTTGGCC 
CTGGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGAGA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAAGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CCCGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC ATCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGCTGT GTGCTCCTGC CTTCTTCCTG 
CAGGGCCTTC TCCAAACGTC TGAGGACACA GACCTGCCCT ACCCACCACC CCAGAGGGAG 
GCCAACATCT ACATGGTTCC TCAGAACATC AAGCCAGCGC TCCAGCGTAC CGCCATTGAG 
ATCCTGGCAT GGGGCCTGCG GAACATGAAG AGTTACCAGC TGGCCAACAT CTCCTCCCCC 
AGCCTCGTGG TAGAGTGTCG GGGCCAGACG GTGCAGTCCT GTGTCATCAG GAACCTCCGG 
AAGAACCCCA ACTTTGACAT CTGCACCCTC TTCATGGAAG TGATGCTGCC CAGGGAGGAG 
CTCTACTGCC CCCCCATCAC CGTCAAGGTC ATCGATAACC GCCAGTTTGG CCGCCGGCCT 
GTGGTGGGCC AGTGTACCAT CCGCTCCCTG GAGAGCTTCC TGTGTGACCC CTACTCGGCG 
GAGAGTCCAT CCCCACAGGG TGGCCCAGAC GATGTGAGCC TACTCAGTCC TGGGGAAGAC 
GTGCTCATCG ACATTGATGA CAAGGAGCCC CTCATCCCCA TCCAGCTTGC AGACGGTCTG 
TCGAGCTTGG CCCCCACTAA CACGGCTTCT CCTCCATCCA GTCCTCATAA GACCCTCCGG 
GAGGAAGAGT TCATCGATTG GTGGAGCAAA TTCTTTGCCT CCATAGGGGA GAGGGAAAAG 
TGCGGCTCCT ACTTGGAGAA GGATTTTGAC ACCCTGAAGG TCTATGACAC ACAGCTGGAG 
AATGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT GTACCGGGGC 
AAGACGCAGG AGGAGACAGA AGATCCATCT GTCATTGGTG AATTTAAGGG CCTCTTCAAA 
ATTTATCCCC TCCCAGAAGA CCCAGCCATC CCCATGCCCC CAAGACAGTT CCACCAGCTG 
GCCGCCCAGG GACCCCAGGA GTGCTTGGTC CGTATCTACA TTGTCCGAGC ATTTGGCCTG 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATCCCC TGCACGCTGG AGCCCGTATT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTAA AGATCACTCT CTATGACTAT 
GACCTCCTCT CCAAGGACGA AAAGATCGGT GAGACGGTCA TCGACCTGGA AAACAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGTGGACTC CCACAGACCT ACTGTGTCTC TGGACCGAAC 
CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCATAGA 
GTCAAGGCAC CTGTGTACCG GACAGACCGT GTAATGTTTC AGGATAAAGA ATATTCCATT 
GAAGAGATAG AGGCTGGCAG GATCCCAAAC CCACACCTGG GCCCAGTGGA GGAGCGTCTG 
GCTCTGCATG TGCTTCAGCA GCAGGGCCTG GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC 
TACAGCCCCC TGCAGCCAGA CATCGAGCAG GGGAAGCTGC AGATGTGGGT CGACCTATTT 
CCGAAGGCCC TGGGGCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAATACC AGAGATGTGA TCCTGGATGA CCTGAGCCTC 
ACGGGGGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGATTGGCTT TGAAGAACAC 
AAGCAAAAGA CAGACGTGCA TTATCGTTCC CTGGGAGGTG AAGGCAACTT CAACTGGAGG 
TTCATTTTCC CCTTCGACTA CCTGCCAGCT GAGCAAGTCT GTACCATTGC CAAGAAGGAT 
GCCTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT GTTCCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGATGAT TTTCTGGGCT CCCTGCAGCT CGATCTCAAC 
CGCATGCCCA AGCCAGCCAA GACAGCCGAG AAGTGCTCCT TGGACCAGCT GGATGATGCT 
TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC 
TGTGTAGCAG AAGAGGGTGA GAAGAAAATA CTGGCGGGCA AGCTGGAAAT GACCTTGGAG 
ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC 
ATGAACCCTA AGCTTGAGGA CCCAAGGCGC CCCGACACCT CCTTCCTGTG GTTTACCTCC 
CCATACAAGA CCATGAAGTT CATCCTGTGG CGGCGTTTCC GGTGGGCCAT CATCCTCTTC 
ATCATCCTCT TCATCCTGCT GCTGTTCCTG GCCATCTTCA TCTACGCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948747.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804236.1 Pan troglodytes dysferlin (DYSF), transcript variant X19, mRNA.

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CloneID	OPc103434
Clone ID Related Accession (Same CDS sequence)	XM_016948747.2
Accession Version	XM_016948747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGCTGTGTG CTCCTGCCTT CTTCCTGCAG GGCCTTCTCC AAACGTCTGA GGACACAGAC 
CTGCCCTACC CACCACCCCA GAGGGAGGCC AACATCTACA TGGTTCCTCA GAACATCAAG 
CCAGCGCTCC AGCGTACCGC CATTGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT 
TACCAGCTGG CCAACATCTC CTCCCCCAGC CTCGTGGTAG AGTGTCGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TTGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGCCCCC CCATCACCGT CAAGGTCATC 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCCTA CTCGGCGGAG AGTCCATCCC CACAGGGTGG CCCAGACGAT 
GTGAGCCTAC TCAGTCCTGG GGAAGACGTG CTCATCGACA TTGATGACAA GGAGCCCCTC 
ATCCCCATCC AGCTTGCAGA CGGTCTGTCG AGCTTGGCCC CCACTAACAC GGCTTCTCCT 
CCATCCAGTC CTCATAAGAC CCTCCGGGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCATCG ACCTGGAAAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804236.1
CDS	891..7250
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948747.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948747.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGCTGTGTG CTCCTGCCTT CTTCCTGCAG GGCCTTCTCC AAACGTCTGA GGACACAGAC 
CTGCCCTACC CACCACCCCA GAGGGAGGCC AACATCTACA TGGTTCCTCA GAACATCAAG 
CCAGCGCTCC AGCGTACCGC CATTGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT 
TACCAGCTGG CCAACATCTC CTCCCCCAGC CTCGTGGTAG AGTGTCGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TTGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGCCCCC CCATCACCGT CAAGGTCATC 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCCTA CTCGGCGGAG AGTCCATCCC CACAGGGTGG CCCAGACGAT 
GTGAGCCTAC TCAGTCCTGG GGAAGACGTG CTCATCGACA TTGATGACAA GGAGCCCCTC 
ATCCCCATCC AGCTTGCAGA CGGTCTGTCG AGCTTGGCCC CCACTAACAC GGCTTCTCCT 
CCATCCAGTC CTCATAAGAC CCTCCGGGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCATCG ACCTGGAAAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948748.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804237.1 Pan troglodytes dysferlin (DYSF), transcript variant X20, mRNA.

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CloneID	OPc103435
Clone ID Related Accession (Same CDS sequence)	XM_016948748.2
Accession Version	XM_016948748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAT 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGTC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TCCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACCA AGTTGGCCCT GGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGAGAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA AGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCC CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCATCGACC TGGAAAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804237.1
CDS	890..7246
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948748.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948748.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAT 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGTC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TCCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACCA AGTTGGCCCT GGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGAGAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA AGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCC CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCATCGACC TGGAAAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948749.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804238.1 Pan troglodytes dysferlin (DYSF), transcript variant X21, mRNA.

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CloneID	OPc103436
Clone ID Related Accession (Same CDS sequence)	XM_016948749.2
Accession Version	XM_016948749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ATGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCG TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC CAAGTTGGCC 
CTGGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGAGA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAAGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CCCGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC ATCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTCGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCATCGACCT GGAAAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804238.1
CDS	890..7225
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948749.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948749.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ATGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCG TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC CAAGTTGGCC 
CTGGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGAGA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAAGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CCCGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC ATCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTCGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCATCGACCT GGAAAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_024354432.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024210200.1 Pan troglodytes dysferlin (DYSF), transcript variant X22, mRNA.

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CloneID	OPc103437
Clone ID Related Accession (Same CDS sequence)	XM_024354432.1
Accession Version	XM_024354432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATAAGACCC TCCGGGAGGA AGAGTTCATC 
GATTGGTGGA GCAAATTCTT TGCCTCCATA GGGGAGAGGG AAAAGTGCGG CTCCTACTTG 
GAGAAGGATT TTGACACCCT GAAGGTCTAT GACACACAGC TGGAGAATGT GGAGGCCTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTGTACC GGGGCAAGAC GCAGGAGGAG 
ACAGAAGATC CATCTGTCAT TGGTGAATTT AAGGGCCTCT TCAAAATTTA TCCCCTCCCA 
GAAGACCCAG CCATCCCCAT GCCCCCAAGA CAGTTCCACC AGCTGGCCGC CCAGGGACCC 
CAGGAGTGCT TGGTCCGTAT CTACATTGTC CGAGCATTTG GCCTGCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG 
GATAACTACA TCCCCTGCAC GCTGGAGCCC GTATTTGGAA AGATGTTCGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTAAAGATC ACTCTCTATG ACTATGACCT CCTCTCCAAG 
GACGAAAAGA TCGGTGAGAC GGTCATCGAC CTGGAAAACA GGCTGCTGTC CAAGTTTGGG 
GCTCGCTGTG GACTCCCACA GACCTACTGT GTCTCTGGAC CGAACCAGTG GCGGGACCAG 
CTCCGCCCCT CCCAGCTCCT CCACCTCTTC TGCCAGCAGC ATAGAGTCAA GGCACCTGTG 
TACCGGACAG ACCGTGTAAT GTTTCAGGAT AAAGAATATT CCATTGAAGA GATAGAGGCT 
GGCAGGATCC CAAACCCACA CCTGGGCCCA GTGGAGGAGC GTCTGGCTCT GCATGTGCTT 
CAGCAGCAGG GCCTGGTCCC GGAGCACGTG GAGTCACGGC CCCTCTACAG CCCCCTGCAG 
CCAGACATCG AGCAGGGGAA GCTGCAGATG TGGGTCGACC TATTTCCGAA GGCCCTGGGG 
CGGCCTGGAC CTCCCTTCAA CATCACCCCA CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT 
ATTATCTGGA ATACCAGAGA TGTGATCCTG GATGACCTGA GCCTCACGGG GGAGAAGATG 
AGCGACATTT ATGTGAAAGG TTGGATGATT GGCTTTGAAG AACACAAGCA AAAGACAGAC 
GTGCATTATC GTTCCCTGGG AGGTGAAGGC AACTTCAACT GGAGGTTCAT TTTCCCCTTC 
GACTACCTGC CAGCTGAGCA AGTCTGTACC ATTGCCAAGA AGGATGCCTT CTGGAGGCTG 
GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTGTTCC AGATCTGGGA CAATGACAAG 
TTCTCCTTTG ATGATTTTCT GGGCTCCCTG CAGCTCGATC TCAACCGCAT GCCCAAGCCA 
GCCAAGACAG CCGAGAAGTG CTCCTTGGAC CAGCTGGATG ATGCTTTCCA CCCAGAATGG 
TTTGTGTCCC TTTTTGAGCA GAAAACAGTG AAGGGCTGGT GGCCCTGTGT AGCAGAAGAG 
GGTGAGAAGA AAATACTGGC GGGCAAGCTG GAAATGACCT TGGAGATTGT AGCAGAGAGT 
GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAGC CCAACATGAA CCCTAAGCTT 
GAGGACCCAA GGCGCCCCGA CACCTCCTTC CTGTGGTTTA CCTCCCCATA CAAGACCATG 
AAGTTCATCC TGTGGCGGCG TTTCCGGTGG GCCATCATCC TCTTCATCAT CCTCTTCATC 
CTGCTGCTGT TCCTGGCCAT CTTCATCTAC GCCTTCCCGA ACTATGCTGC CATGAAGCTG 
GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024210200.1
CDS	890..7207
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_024354432.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024354432.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATAAGACCC TCCGGGAGGA AGAGTTCATC 
GATTGGTGGA GCAAATTCTT TGCCTCCATA GGGGAGAGGG AAAAGTGCGG CTCCTACTTG 
GAGAAGGATT TTGACACCCT GAAGGTCTAT GACACACAGC TGGAGAATGT GGAGGCCTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTGTACC GGGGCAAGAC GCAGGAGGAG 
ACAGAAGATC CATCTGTCAT TGGTGAATTT AAGGGCCTCT TCAAAATTTA TCCCCTCCCA 
GAAGACCCAG CCATCCCCAT GCCCCCAAGA CAGTTCCACC AGCTGGCCGC CCAGGGACCC 
CAGGAGTGCT TGGTCCGTAT CTACATTGTC CGAGCATTTG GCCTGCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG 
GATAACTACA TCCCCTGCAC GCTGGAGCCC GTATTTGGAA AGATGTTCGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTAAAGATC ACTCTCTATG ACTATGACCT CCTCTCCAAG 
GACGAAAAGA TCGGTGAGAC GGTCATCGAC CTGGAAAACA GGCTGCTGTC CAAGTTTGGG 
GCTCGCTGTG GACTCCCACA GACCTACTGT GTCTCTGGAC CGAACCAGTG GCGGGACCAG 
CTCCGCCCCT CCCAGCTCCT CCACCTCTTC TGCCAGCAGC ATAGAGTCAA GGCACCTGTG 
TACCGGACAG ACCGTGTAAT GTTTCAGGAT AAAGAATATT CCATTGAAGA GATAGAGGCT 
GGCAGGATCC CAAACCCACA CCTGGGCCCA GTGGAGGAGC GTCTGGCTCT GCATGTGCTT 
CAGCAGCAGG GCCTGGTCCC GGAGCACGTG GAGTCACGGC CCCTCTACAG CCCCCTGCAG 
CCAGACATCG AGCAGGGGAA GCTGCAGATG TGGGTCGACC TATTTCCGAA GGCCCTGGGG 
CGGCCTGGAC CTCCCTTCAA CATCACCCCA CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT 
ATTATCTGGA ATACCAGAGA TGTGATCCTG GATGACCTGA GCCTCACGGG GGAGAAGATG 
AGCGACATTT ATGTGAAAGG TTGGATGATT GGCTTTGAAG AACACAAGCA AAAGACAGAC 
GTGCATTATC GTTCCCTGGG AGGTGAAGGC AACTTCAACT GGAGGTTCAT TTTCCCCTTC 
GACTACCTGC CAGCTGAGCA AGTCTGTACC ATTGCCAAGA AGGATGCCTT CTGGAGGCTG 
GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTGTTCC AGATCTGGGA CAATGACAAG 
TTCTCCTTTG ATGATTTTCT GGGCTCCCTG CAGCTCGATC TCAACCGCAT GCCCAAGCCA 
GCCAAGACAG CCGAGAAGTG CTCCTTGGAC CAGCTGGATG ATGCTTTCCA CCCAGAATGG 
TTTGTGTCCC TTTTTGAGCA GAAAACAGTG AAGGGCTGGT GGCCCTGTGT AGCAGAAGAG 
GGTGAGAAGA AAATACTGGC GGGCAAGCTG GAAATGACCT TGGAGATTGT AGCAGAGAGT 
GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAGC CCAACATGAA CCCTAAGCTT 
GAGGACCCAA GGCGCCCCGA CACCTCCTTC CTGTGGTTTA CCTCCCCATA CAAGACCATG 
AAGTTCATCC TGTGGCGGCG TTTCCGGTGG GCCATCATCC TCTTCATCAT CCTCTTCATC 
CTGCTGCTGT TCCTGGCCAT CTTCATCTAC GCCTTCCCGA ACTATGCTGC CATGAAGCTG 
GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948750.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804239.1 Pan troglodytes dysferlin (DYSF), transcript variant X23, mRNA.

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CloneID	OPc103438
Clone ID Related Accession (Same CDS sequence)	XM_016948750.2
Accession Version	XM_016948750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCATCGACCT GGAAAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804239.1
CDS	890..7195
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948750.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948750.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCATCGACCT GGAAAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948751.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804240.1 Pan troglodytes dysferlin (DYSF), transcript variant X24, mRNA.

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CloneID	OPc103439
Clone ID Related Accession (Same CDS sequence)	XM_016948751.2
Accession Version	XM_016948751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAT 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGTC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TCCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACCA AGTTGGCCCT GGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGAGAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA AGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCC CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804240.1
CDS	890..7183
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948751.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948751.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAT 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGTC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TCCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACCA AGTTGGCCCT GGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGAGAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA AGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCC CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948752.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804241.1 Pan troglodytes dysferlin (DYSF), transcript variant X25, mRNA.

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CloneID	OPc103440
Clone ID Related Accession (Same CDS sequence)	XM_016948752.2
Accession Version	XM_016948752.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGATGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCGTCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTCC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACCAAGT TGGCCCTGGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGAGAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAAGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCCCGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCATCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804241.1
CDS	889..7152
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948752.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948752.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGATGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCGTCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTCC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACCAAGT TGGCCCTGGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGAGAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAAGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCCCGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCATCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948753.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804242.1 Pan troglodytes dysferlin (DYSF), transcript variant X26, mRNA.

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CloneID	OPc103441
Clone ID Related Accession (Same CDS sequence)	XM_016948753.2
Accession Version	XM_016948753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCA TCGACCTGGA AAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804242.1
CDS	889..7131
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948753.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948753.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCA TCGACCTGGA AAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948754.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804243.1 Pan troglodytes dysferlin (DYSF), transcript variant X27, mRNA.

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CloneID	OPc103442
Clone ID Related Accession (Same CDS sequence)	XM_016948754.2
Accession Version	XM_016948754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016948754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGATGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCGTCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTCC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACCAAGT TGGCCCTGGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGAGAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAAGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCCCGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCATCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCATC GACCTGGAAA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804243.1
CDS	889..7089
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948754.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948754.2

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGATGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCGTCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTCC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACCAAGT TGGCCCTGGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGAGAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAAGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCCCGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCATCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCATC GACCTGGAAA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948729.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804218.1 Pan troglodytes dysferlin (DYSF), transcript variant X1, mRNA.

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CloneID	OPc79268
Clone ID Related Accession (Same CDS sequence)	XM_016948729.1
Accession Version	XM_016948729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TAAGACCCTC 
CGGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCATCGACCT GGAAAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804218.1
CDS	161..6616
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948729.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948729.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TAAGACCCTC 
CGGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCATCGACCT GGAAAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948730.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804219.1 Pan troglodytes dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OPc79269
Clone ID Related Accession (Same CDS sequence)	XM_016948730.1
Accession Version	XM_016948730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG AGACATCAAG GATCCTGGAT GAGCTGTGTG CTCCTGCCTT CTTCCTGCAG 
GGCCTTCTCC AAACGTCTGA GGACACAGAC CTGCCCTACC CACCACCCCA GAGGGAGGCC 
AACATCTACA TGGTTCCTCA GAACATCAAG CCAGCGCTCC AGCGTACCGC CATTGAGATC 
CTGGCATGGG GCCTGCGGAA CATGAAGAGT TACCAGCTGG CCAACATCTC CTCCCCCAGC 
CTCGTGGTAG AGTGTCGGGG CCAGACGGTG CAGTCCTGTG TCATCAGGAA CCTCCGGAAG 
AACCCCAACT TTGACATCTG CACCCTCTTC ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC 
TACTGCCCCC CCATCACCGT CAAGGTCATC GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG CTCCCTGGAG AGCTTCCTGT GTGACCCCTA CTCGGCGGAG 
AGTCCATCCC CACAGGGTGG CCCAGACGAT GTGAGCCTAC TCAGTCCTGG GGAAGACGTG 
CTCATCGACA TTGATGACAA GGAGCCCCTC ATCCCCATCC AGCTTGCAGA CGGTCTGTCG 
AGCTTGGCCC CCACTAACAC GGCTTCTCCT CCATCCAGTC CTCATAAGAC CCTCCGGGAG 
GAAGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC 
GGCTCCTACT TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTGTA CCGGGGCAAG 
ACGCAGGAGG AGACAGAAGA TCCATCTGTC ATTGGTGAAT TTAAGGGCCT CTTCAAAATT 
TATCCCCTCC CAGAAGACCC AGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTGGCC 
GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT ATCTACATTG TCCGAGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCCTGC ACGCTGGAGC CCGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTAAAGA TCACTCTCTA TGACTATGAC 
CTCCTCTCCA AGGACGAAAA GATCGGTGAG ACGGTCATCG ACCTGGAAAA CAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG 
TGGCGGGACC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCATAGAGTC 
AAGGCACCTG TGTACCGGAC AGACCGTGTA ATGTTTCAGG ATAAAGAATA TTCCATTGAA 
GAGATAGAGG CTGGCAGGAT CCCAAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCT 
CTGCATGTGC TTCAGCAGCA GGGCCTGGTC CCGGAGCACG TGGAGTCACG GCCCCTCTAC 
AGCCCCCTGC AGCCAGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTATTTCCG 
AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAATACCAGA GATGTGATCC TGGATGACCT GAGCCTCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGA TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ACGTGCATTA TCGTTCCCTG GGAGGTGAAG GCAACTTCAA CTGGAGGTTC 
ATTTTCCCCT TCGACTACCT GCCAGCTGAG CAAGTCTGTA CCATTGCCAA GAAGGATGCC 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTGTT CCAGATCTGG 
GACAATGACA AGTTCTCCTT TGATGATTTT CTGGGCTCCC TGCAGCTCGA TCTCAACCGC 
ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG TGCTCCTTGG ACCAGCTGGA TGATGCTTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTAGCAGAAG AGGGTGAGAA GAAAATACTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA GCCCAACATG 
AACCCTAAGC TTGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT TACCTCCCCA 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GGGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC ATCTTCATCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804219.1
CDS	161..6610
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948730.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948730.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG AGACATCAAG GATCCTGGAT GAGCTGTGTG CTCCTGCCTT CTTCCTGCAG 
GGCCTTCTCC AAACGTCTGA GGACACAGAC CTGCCCTACC CACCACCCCA GAGGGAGGCC 
AACATCTACA TGGTTCCTCA GAACATCAAG CCAGCGCTCC AGCGTACCGC CATTGAGATC 
CTGGCATGGG GCCTGCGGAA CATGAAGAGT TACCAGCTGG CCAACATCTC CTCCCCCAGC 
CTCGTGGTAG AGTGTCGGGG CCAGACGGTG CAGTCCTGTG TCATCAGGAA CCTCCGGAAG 
AACCCCAACT TTGACATCTG CACCCTCTTC ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC 
TACTGCCCCC CCATCACCGT CAAGGTCATC GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG CTCCCTGGAG AGCTTCCTGT GTGACCCCTA CTCGGCGGAG 
AGTCCATCCC CACAGGGTGG CCCAGACGAT GTGAGCCTAC TCAGTCCTGG GGAAGACGTG 
CTCATCGACA TTGATGACAA GGAGCCCCTC ATCCCCATCC AGCTTGCAGA CGGTCTGTCG 
AGCTTGGCCC CCACTAACAC GGCTTCTCCT CCATCCAGTC CTCATAAGAC CCTCCGGGAG 
GAAGAGTTCA TCGATTGGTG GAGCAAATTC TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC 
GGCTCCTACT TGGAGAAGGA TTTTGACACC CTGAAGGTCT ATGACACACA GCTGGAGAAT 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTGTA CCGGGGCAAG 
ACGCAGGAGG AGACAGAAGA TCCATCTGTC ATTGGTGAAT TTAAGGGCCT CTTCAAAATT 
TATCCCCTCC CAGAAGACCC AGCCATCCCC ATGCCCCCAA GACAGTTCCA CCAGCTGGCC 
GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT ATCTACATTG TCCGAGCATT TGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATCCCCTGC ACGCTGGAGC CCGTATTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTAAAGA TCACTCTCTA TGACTATGAC 
CTCCTCTCCA AGGACGAAAA GATCGGTGAG ACGGTCATCG ACCTGGAAAA CAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG TGGACTCCCA CAGACCTACT GTGTCTCTGG ACCGAACCAG 
TGGCGGGACC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCATAGAGTC 
AAGGCACCTG TGTACCGGAC AGACCGTGTA ATGTTTCAGG ATAAAGAATA TTCCATTGAA 
GAGATAGAGG CTGGCAGGAT CCCAAACCCA CACCTGGGCC CAGTGGAGGA GCGTCTGGCT 
CTGCATGTGC TTCAGCAGCA GGGCCTGGTC CCGGAGCACG TGGAGTCACG GCCCCTCTAC 
AGCCCCCTGC AGCCAGACAT CGAGCAGGGG AAGCTGCAGA TGTGGGTCGA CCTATTTCCG 
AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATTATCTG GAATACCAGA GATGTGATCC TGGATGACCT GAGCCTCACG 
GGGGAGAAGA TGAGCGACAT TTATGTGAAA GGTTGGATGA TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ACGTGCATTA TCGTTCCCTG GGAGGTGAAG GCAACTTCAA CTGGAGGTTC 
ATTTTCCCCT TCGACTACCT GCCAGCTGAG CAAGTCTGTA CCATTGCCAA GAAGGATGCC 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTGTT CCAGATCTGG 
GACAATGACA AGTTCTCCTT TGATGATTTT CTGGGCTCCC TGCAGCTCGA TCTCAACCGC 
ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG TGCTCCTTGG ACCAGCTGGA TGATGCTTTC 
CACCCAGAAT GGTTTGTGTC CCTTTTTGAG CAGAAAACAG TGAAGGGCTG GTGGCCCTGT 
GTAGCAGAAG AGGGTGAGAA GAAAATACTG GCGGGCAAGC TGGAAATGAC CTTGGAGATT 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA GCCCAACATG 
AACCCTAAGC TTGAGGACCC AAGGCGCCCC GACACCTCCT TCCTGTGGTT TACCTCCCCA 
TACAAGACCA TGAAGTTCAT CCTGTGGCGG CGTTTCCGGT GGGCCATCAT CCTCTTCATC 
ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC ATCTTCATCT ACGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948731.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804220.1 Pan troglodytes dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OPc79270
Clone ID Related Accession (Same CDS sequence)	XM_016948731.1
Accession Version	XM_016948731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804220.1
CDS	160..6603
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948731.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948731.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948732.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804221.1 Pan troglodytes dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OPc79271
Clone ID Related Accession (Same CDS sequence)	XM_016948732.1
Accession Version	XM_016948732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATA AGACCCTCCG GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804221.1
CDS	160..6573
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948732.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948732.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATA AGACCCTCCG GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948733.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804222.1 Pan troglodytes dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OPc79272
Clone ID Related Accession (Same CDS sequence)	XM_016948733.1
Accession Version	XM_016948733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGCTG 
TGTGCTCCTG CCTTCTTCCT GCAGGGCCTT CTCCAAACGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATA AGACCCTCCG GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804222.1
CDS	160..6573
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948733.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948733.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGCTG 
TGTGCTCCTG CCTTCTTCCT GCAGGGCCTT CTCCAAACGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATA AGACCCTCCG GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948734.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804223.1 Pan troglodytes dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OPc79273
Clone ID Related Accession (Same CDS sequence)	XM_016948734.1
Accession Version	XM_016948734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG AGACATCAAG GATCCTGGAT GAGTCTGAGG ACACAGACCT GCCCTACCCA 
CCACCCCAGA GGGAGGCCAA CATCTACATG GTTCCTCAGA ACATCAAGCC AGCGCTCCAG 
CGTACCGCCA TTGAGATCCT GGCATGGGGC CTGCGGAACA TGAAGAGTTA CCAGCTGGCC 
AACATCTCCT CCCCCAGCCT CGTGGTAGAG TGTCGGGGCC AGACGGTGCA GTCCTGTGTC 
ATCAGGAACC TCCGGAAGAA CCCCAACTTT GACATCTGCA CCCTCTTCAT GGAAGTGATG 
CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC ATCACCGTCA AGGTCATCGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGCT CCCTGGAGAG CTTCCTGTGT 
GACCCCTACT CGGCGGAGAG TCCATCCCCA CAGGGTGGCC CAGACGATGT GAGCCTACTC 
AGTCCTGGGG AAGACGTGCT CATCGACATT GATGACAAGG AGCCCCTCAT CCCCATCCAG 
CTTGCAGACG GTCTGTCGAG CTTGGCCCCC ACTAACACGG CTTCTCCTCC ATCCAGTCCT 
CATAAGACCC TCCGGGAGGA AGAGTTCATC GATTGGTGGA GCAAATTCTT TGCCTCCATA 
GGGGAGAGGG AAAAGTGCGG CTCCTACTTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTGTACC GGGGCAAGAC GCAGGAGGAG ACAGAAGATC CATCTGTCAT TGGTGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCAG CCATCCCCAT GCCCCCAAGA 
CAGTTCCACC AGCTGGCCGC CCAGGGACCC CAGGAGTGCT TGGTCCGTAT CTACATTGTC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCCTGCAC GCTGGAGCCC 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTAAAGATC 
ACTCTCTATG ACTATGACCT CCTCTCCAAG GACGAAAAGA TCGGTGAGAC GGTCATCGAC 
CTGGAAAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CGAACCAGTG GCGGGACCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC ATAGAGTCAA GGCACCTGTG TACCGGACAG ACCGTGTAAT GTTTCAGGAT 
AAAGAATATT CCATTGAAGA GATAGAGGCT GGCAGGATCC CAAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCTCT GCATGTGCTT CAGCAGCAGG GCCTGGTCCC GGAGCACGTG 
GAGTCACGGC CCCTCTACAG CCCCCTGCAG CCAGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TATTTCCGAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ATACCAGAGA TGTGATCCTG 
GATGACCTGA GCCTCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGATT 
GGCTTTGAAG AACACAAGCA AAAGACAGAC GTGCATTATC GTTCCCTGGG AGGTGAAGGC 
AACTTCAACT GGAGGTTCAT TTTCCCCTTC GACTACCTGC CAGCTGAGCA AGTCTGTACC 
ATTGCCAAGA AGGATGCCTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGGTGTTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTTCT GGGCTCCCTG 
CAGCTCGATC TCAACCGCAT GCCCAAGCCA GCCAAGACAG CCGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ATGCTTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT AGCAGAAGAG GGTGAGAAGA AAATACTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATTGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC 
CGGGATGAGC CCAACATGAA CCCTAAGCTT GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTTA CCTCCCCATA CAAGACCATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGG 
GCCATCATCC TCTTCATCAT CCTCTTCATC CTGCTGCTGT TCCTGGCCAT CTTCATCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804223.1
CDS	160..6567
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948734.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948734.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG AGACATCAAG GATCCTGGAT GAGTCTGAGG ACACAGACCT GCCCTACCCA 
CCACCCCAGA GGGAGGCCAA CATCTACATG GTTCCTCAGA ACATCAAGCC AGCGCTCCAG 
CGTACCGCCA TTGAGATCCT GGCATGGGGC CTGCGGAACA TGAAGAGTTA CCAGCTGGCC 
AACATCTCCT CCCCCAGCCT CGTGGTAGAG TGTCGGGGCC AGACGGTGCA GTCCTGTGTC 
ATCAGGAACC TCCGGAAGAA CCCCAACTTT GACATCTGCA CCCTCTTCAT GGAAGTGATG 
CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC ATCACCGTCA AGGTCATCGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGCT CCCTGGAGAG CTTCCTGTGT 
GACCCCTACT CGGCGGAGAG TCCATCCCCA CAGGGTGGCC CAGACGATGT GAGCCTACTC 
AGTCCTGGGG AAGACGTGCT CATCGACATT GATGACAAGG AGCCCCTCAT CCCCATCCAG 
CTTGCAGACG GTCTGTCGAG CTTGGCCCCC ACTAACACGG CTTCTCCTCC ATCCAGTCCT 
CATAAGACCC TCCGGGAGGA AGAGTTCATC GATTGGTGGA GCAAATTCTT TGCCTCCATA 
GGGGAGAGGG AAAAGTGCGG CTCCTACTTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTGTACC GGGGCAAGAC GCAGGAGGAG ACAGAAGATC CATCTGTCAT TGGTGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCAG CCATCCCCAT GCCCCCAAGA 
CAGTTCCACC AGCTGGCCGC CCAGGGACCC CAGGAGTGCT TGGTCCGTAT CTACATTGTC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCCTGCAC GCTGGAGCCC 
GTATTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTAAAGATC 
ACTCTCTATG ACTATGACCT CCTCTCCAAG GACGAAAAGA TCGGTGAGAC GGTCATCGAC 
CTGGAAAACA GGCTGCTGTC CAAGTTTGGG GCTCGCTGTG GACTCCCACA GACCTACTGT 
GTCTCTGGAC CGAACCAGTG GCGGGACCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC ATAGAGTCAA GGCACCTGTG TACCGGACAG ACCGTGTAAT GTTTCAGGAT 
AAAGAATATT CCATTGAAGA GATAGAGGCT GGCAGGATCC CAAACCCACA CCTGGGCCCA 
GTGGAGGAGC GTCTGGCTCT GCATGTGCTT CAGCAGCAGG GCCTGGTCCC GGAGCACGTG 
GAGTCACGGC CCCTCTACAG CCCCCTGCAG CCAGACATCG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TATTTCCGAA GGCCCTGGGG CGGCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATTATCTGGA ATACCAGAGA TGTGATCCTG 
GATGACCTGA GCCTCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGATT 
GGCTTTGAAG AACACAAGCA AAAGACAGAC GTGCATTATC GTTCCCTGGG AGGTGAAGGC 
AACTTCAACT GGAGGTTCAT TTTCCCCTTC GACTACCTGC CAGCTGAGCA AGTCTGTACC 
ATTGCCAAGA AGGATGCCTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGGTGTTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTTCT GGGCTCCCTG 
CAGCTCGATC TCAACCGCAT GCCCAAGCCA GCCAAGACAG CCGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ATGCTTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAAACAGTG 
AAGGGCTGGT GGCCCTGTGT AGCAGAAGAG GGTGAGAAGA AAATACTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATTGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC 
CGGGATGAGC CCAACATGAA CCCTAAGCTT GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTTA CCTCCCCATA CAAGACCATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGG 
GCCATCATCC TCTTCATCAT CCTCTTCATC CTGCTGCTGT TCCTGGCCAT CTTCATCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948735.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804224.1 Pan troglodytes dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OPc79274
Clone ID Related Accession (Same CDS sequence)	XM_016948735.1
Accession Version	XM_016948735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGTGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCCCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGCTGGA GCCCGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTAAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAGGACGAA AAGATCGGTG AGACGGTCAT CGACCTGGAA 
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCATAGAG TCAAGGCACC TGTGTACCGG ACAGACCGTG TAATGTTTCA GGATAAAGAA 
TATTCCATTG AAGAGATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCAGCAG CAGGGCCTGG TCCCGGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTATTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGTTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCAGCTG AGCAAGTCTG TACCATTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTG 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGATGCTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAAATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTTACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804224.1
CDS	159..6560
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948735.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948735.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGTGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCCCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGCTGGA GCCCGTATTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTAAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAGGACGAA AAGATCGGTG AGACGGTCAT CGACCTGGAA 
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCATAGAG TCAAGGCACC TGTGTACCGG ACAGACCGTG TAATGTTTCA GGATAAAGAA 
TATTCCATTG AAGAGATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCAGTGGAG 
GAGCGTCTGG CTCTGCATGT GCTTCAGCAG CAGGGCCTGG TCCCGGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTATTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGTTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCAGCTG AGCAAGTCTG TACCATTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTG 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGATGCTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAAATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTTACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948736.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804225.1 Pan troglodytes dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OPc79275
Clone ID Related Accession (Same CDS sequence)	XM_016948736.1
Accession Version	XM_016948736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG GCCTTCTCCA AACGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATAAGACC 
CTCCGGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCATCGA CCTGGAAAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804225.1
CDS	160..6558
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948736.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948736.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG GCCTTCTCCA AACGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATAAGACC 
CTCCGGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCATCGA CCTGGAAAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948737.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804226.1 Pan troglodytes dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OPc79276
Clone ID Related Accession (Same CDS sequence)	XM_016948737.1
Accession Version	XM_016948737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCATC GACCTGGAAA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804226.1
CDS	160..6540
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948737.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948737.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTGCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCATC GACCTGGAAA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948738.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804227.1 Pan troglodytes dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OPc79277
Clone ID Related Accession (Same CDS sequence)	XM_016948738.1
Accession Version	XM_016948738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGCTGT GTGCTCCTGC CTTCTTCCTG CAGGGCCTTC TCCAAACGTC TGAGGACACA 
GACCTGCCCT ACCCACCACC CCAGAGGGAG GCCAACATCT ACATGGTTCC TCAGAACATC 
AAGCCAGCGC TCCAGCGTAC CGCCATTGAG ATCCTGGCAT GGGGCCTGCG GAACATGAAG 
AGTTACCAGC TGGCCAACAT CTCCTCCCCC AGCCTCGTGG TAGAGTGTCG GGGCCAGACG 
GTGCAGTCCT GTGTCATCAG GAACCTCCGG AAGAACCCCA ACTTTGACAT CTGCACCCTC 
TTCATGGAAG TGATGCTGCC CAGGGAGGAG CTCTACTGCC CCCCCATCAC CGTCAAGGTC 
ATCGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGCTCCCTG 
GAGAGCTTCC TGTGTGACCC CTACTCGGCG GAGAGTCCAT CCCCACAGGG TGGCCCAGAC 
GATGTGAGCC TACTCAGTCC TGGGGAAGAC GTGCTCATCG ACATTGATGA CAAGGAGCCC 
CTCATCCCCA TCCAGCTTGC AGACGGTCTG TCGAGCTTGG CCCCCACTAA CACGGCTTCT 
CCTCCATCCA GTCCTCATAA GACCCTCCGG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCA TCGACCTGGA AAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804227.1
CDS	160..6522
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948738.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948738.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGCTGT GTGCTCCTGC CTTCTTCCTG CAGGGCCTTC TCCAAACGTC TGAGGACACA 
GACCTGCCCT ACCCACCACC CCAGAGGGAG GCCAACATCT ACATGGTTCC TCAGAACATC 
AAGCCAGCGC TCCAGCGTAC CGCCATTGAG ATCCTGGCAT GGGGCCTGCG GAACATGAAG 
AGTTACCAGC TGGCCAACAT CTCCTCCCCC AGCCTCGTGG TAGAGTGTCG GGGCCAGACG 
GTGCAGTCCT GTGTCATCAG GAACCTCCGG AAGAACCCCA ACTTTGACAT CTGCACCCTC 
TTCATGGAAG TGATGCTGCC CAGGGAGGAG CTCTACTGCC CCCCCATCAC CGTCAAGGTC 
ATCGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGCTCCCTG 
GAGAGCTTCC TGTGTGACCC CTACTCGGCG GAGAGTCCAT CCCCACAGGG TGGCCCAGAC 
GATGTGAGCC TACTCAGTCC TGGGGAAGAC GTGCTCATCG ACATTGATGA CAAGGAGCCC 
CTCATCCCCA TCCAGCTTGC AGACGGTCTG TCGAGCTTGG CCCCCACTAA CACGGCTTCT 
CCTCCATCCA GTCCTCATAA GACCCTCCGG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCA TCGACCTGGA AAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948739.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804228.1 Pan troglodytes dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OPc79278
Clone ID Related Accession (Same CDS sequence)	XM_016948739.1
Accession Version	XM_016948739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTCGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCATCG ACCTGGAAAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804228.1
CDS	159..6518
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948739.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948739.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTCGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCATCG ACCTGGAAAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948740.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804229.1 Pan troglodytes dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OPc79279
Clone ID Related Accession (Same CDS sequence)	XM_016948740.1
Accession Version	XM_016948740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCATCGA CCTGGAAAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804229.1
CDS	159..6497
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948740.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948740.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG 
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGATGGGTG CCACTACTAC 
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG 
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT 
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC 
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGCTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTCCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACCAAGTTG 
GCCCTGGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACG 
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG AGAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAAGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT 
CTGGAACGGA TGCCCCGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCATCGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATTGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTC GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGTGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCATCGA CCTGGAAAAC 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT 
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG 
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948741.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804230.1 Pan troglodytes dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OPc79280
Clone ID Related Accession (Same CDS sequence)	XM_016948741.1
Accession Version	XM_016948741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTCGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG 
ATCGGTGAGA CGGTCATCGA CCTGGAAAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA 
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804230.1
CDS	159..6467
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948741.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948741.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTCGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG 
ATCGGTGAGA CGGTCATCGA CCTGGAAAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA 
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG 
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948742.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804231.1 Pan troglodytes dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OPc79281
Clone ID Related Accession (Same CDS sequence)	XM_016948742.1
Accession Version	XM_016948742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTCGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCATCGACC TGGAAAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804231.1
CDS	159..6455
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948742.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948742.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG 
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTCATCAC GGTGGTAGAC 
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC 
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGACCGC 
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCACCT 
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC CAGACCCCTA CACAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT 
GATGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG 
CTGCGCACCC GTCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC 
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG 
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGCTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTCCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACGAGA CCAAGTTGGC CCTGGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG 
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAG 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACGATT 
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAAGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC 
TGCATCTGTC AACCGAGTCT GGAACGGATG CCCCGGCTGG CCTGGTTCCC ACTGACGAGG 
GGCAGCCAGC CATCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATTGAGA TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTCGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCATCGACC TGGAAAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948743.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804232.1 Pan troglodytes dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	OPc79282
Clone ID Related Accession (Same CDS sequence)	XM_016948743.1
Accession Version	XM_016948743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAT GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCGTC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TCCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACCA AGTTGGCCCT GGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGAGAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA AGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTT ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCC 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCATCGACC TGGAAAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG 
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804232.1
CDS	159..6425
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948743.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948743.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAT GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCGTC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TCCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACCA AGTTGGCCCT GGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGAGAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA AGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTT ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCC 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCATCGACC TGGAAAACAG GCTGCTGTCC 
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG 
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG 
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG 
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA 
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948744.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804233.1 Pan troglodytes dysferlin (DYSF), transcript variant X16, mRNA.

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CloneID	OPc79283
Clone ID Related Accession (Same CDS sequence)	XM_016948744.1
Accession Version	XM_016948744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTCGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCATCGACCT GGAAAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804233.1
CDS	158..6403
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948744.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948744.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT 
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCC AGACCCCTAC 
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCAGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC 
CTGTACCAGC TGCGCACCCG TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG 
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACGAGAC CAAGTTGGCC CTGGTTGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC 
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG 
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG 
ATGGATGAGA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAAGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG 
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT 
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CCCGGCTGGC CTGGTTCCCA 
CTGACGAGGG GCAGCCAGCC ATCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATTGAGAT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA 
GAGTGTCGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCATCGACCT GGAAAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948745.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804234.1 Pan troglodytes dysferlin (DYSF), transcript variant X17, mRNA.

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CloneID	OPc79284
Clone ID Related Accession (Same CDS sequence)	XM_016948745.1
Accession Version	XM_016948745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAT GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCGTC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TCCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACCA AGTTGGCCCT GGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGAGAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA AGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTT ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCC 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804234.1
CDS	158..6361
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948745.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948745.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCCTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT 
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT 
GAGCCCATCT TCATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA 
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC 
CTGAGTATGT CGAAAATCTC TGCACCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ACAGCCGTGC AGTCTTTGAT GGGTGCCACT ACTACTACCT ACCCTGGGGT 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAC ACCCTCTGCC 
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCGTC ACCTGAGCCA AATCACTGAG 
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC 
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TCCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACCA AGTTGGCCCT GGTTGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACGGGCAA GATCAAGCTA 
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG 
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG 
GGGGCCCTGG GCGGCGTGAT GGATGAGAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA AGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTT ATCTTCTACG AGATCGAGAT CTTTGGCGAG 
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCC 
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATTGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC 
TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948746.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804235.1 Pan troglodytes dysferlin (DYSF), transcript variant X18, mRNA.

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CloneID	OPc79285
Clone ID Related Accession (Same CDS sequence)	XM_016948746.1
Accession Version	XM_016948746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ATGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCG TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC CAAGTTGGCC 
CTGGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGAGA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAAGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TTATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CCCGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC ATCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGCTGT GTGCTCCTGC CTTCTTCCTG 
CAGGGCCTTC TCCAAACGTC TGAGGACACA GACCTGCCCT ACCCACCACC CCAGAGGGAG 
GCCAACATCT ACATGGTTCC TCAGAACATC AAGCCAGCGC TCCAGCGTAC CGCCATTGAG 
ATCCTGGCAT GGGGCCTGCG GAACATGAAG AGTTACCAGC TGGCCAACAT CTCCTCCCCC 
AGCCTCGTGG TAGAGTGTCG GGGCCAGACG GTGCAGTCCT GTGTCATCAG GAACCTCCGG 
AAGAACCCCA ACTTTGACAT CTGCACCCTC TTCATGGAAG TGATGCTGCC CAGGGAGGAG 
CTCTACTGCC CCCCCATCAC CGTCAAGGTC ATCGATAACC GCCAGTTTGG CCGCCGGCCT 
GTGGTGGGCC AGTGTACCAT CCGCTCCCTG GAGAGCTTCC TGTGTGACCC CTACTCGGCG 
GAGAGTCCAT CCCCACAGGG TGGCCCAGAC GATGTGAGCC TACTCAGTCC TGGGGAAGAC 
GTGCTCATCG ACATTGATGA CAAGGAGCCC CTCATCCCCA TCCAGCTTGC AGACGGTCTG 
TCGAGCTTGG CCCCCACTAA CACGGCTTCT CCTCCATCCA GTCCTCATAA GACCCTCCGG 
GAGGAAGAGT TCATCGATTG GTGGAGCAAA TTCTTTGCCT CCATAGGGGA GAGGGAAAAG 
TGCGGCTCCT ACTTGGAGAA GGATTTTGAC ACCCTGAAGG TCTATGACAC ACAGCTGGAG 
AATGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT GTACCGGGGC 
AAGACGCAGG AGGAGACAGA AGATCCATCT GTCATTGGTG AATTTAAGGG CCTCTTCAAA 
ATTTATCCCC TCCCAGAAGA CCCAGCCATC CCCATGCCCC CAAGACAGTT CCACCAGCTG 
GCCGCCCAGG GACCCCAGGA GTGCTTGGTC CGTATCTACA TTGTCCGAGC ATTTGGCCTG 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATCCCC TGCACGCTGG AGCCCGTATT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTAA AGATCACTCT CTATGACTAT 
GACCTCCTCT CCAAGGACGA AAAGATCGGT GAGACGGTCA TCGACCTGGA AAACAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGTGGACTC CCACAGACCT ACTGTGTCTC TGGACCGAAC 
CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCATAGA 
GTCAAGGCAC CTGTGTACCG GACAGACCGT GTAATGTTTC AGGATAAAGA ATATTCCATT 
GAAGAGATAG AGGCTGGCAG GATCCCAAAC CCACACCTGG GCCCAGTGGA GGAGCGTCTG 
GCTCTGCATG TGCTTCAGCA GCAGGGCCTG GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC 
TACAGCCCCC TGCAGCCAGA CATCGAGCAG GGGAAGCTGC AGATGTGGGT CGACCTATTT 
CCGAAGGCCC TGGGGCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAATACC AGAGATGTGA TCCTGGATGA CCTGAGCCTC 
ACGGGGGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGATTGGCTT TGAAGAACAC 
AAGCAAAAGA CAGACGTGCA TTATCGTTCC CTGGGAGGTG AAGGCAACTT CAACTGGAGG 
TTCATTTTCC CCTTCGACTA CCTGCCAGCT GAGCAAGTCT GTACCATTGC CAAGAAGGAT 
GCCTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT GTTCCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGATGAT TTTCTGGGCT CCCTGCAGCT CGATCTCAAC 
CGCATGCCCA AGCCAGCCAA GACAGCCGAG AAGTGCTCCT TGGACCAGCT GGATGATGCT 
TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC 
TGTGTAGCAG AAGAGGGTGA GAAGAAAATA CTGGCGGGCA AGCTGGAAAT GACCTTGGAG 
ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC 
ATGAACCCTA AGCTTGAGGA CCCAAGGCGC CCCGACACCT CCTTCCTGTG GTTTACCTCC 
CCATACAAGA CCATGAAGTT CATCCTGTGG CGGCGTTTCC GGTGGGCCAT CATCCTCTTC 
ATCATCCTCT TCATCCTGCT GCTGTTCCTG GCCATCTTCA TCTACGCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804235.1
CDS	893..7345
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948746.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948746.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ATGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCG TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC CAAGTTGGCC 
CTGGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGAGA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAAGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TTATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CCCGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC ATCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGCTGT GTGCTCCTGC CTTCTTCCTG 
CAGGGCCTTC TCCAAACGTC TGAGGACACA GACCTGCCCT ACCCACCACC CCAGAGGGAG 
GCCAACATCT ACATGGTTCC TCAGAACATC AAGCCAGCGC TCCAGCGTAC CGCCATTGAG 
ATCCTGGCAT GGGGCCTGCG GAACATGAAG AGTTACCAGC TGGCCAACAT CTCCTCCCCC 
AGCCTCGTGG TAGAGTGTCG GGGCCAGACG GTGCAGTCCT GTGTCATCAG GAACCTCCGG 
AAGAACCCCA ACTTTGACAT CTGCACCCTC TTCATGGAAG TGATGCTGCC CAGGGAGGAG 
CTCTACTGCC CCCCCATCAC CGTCAAGGTC ATCGATAACC GCCAGTTTGG CCGCCGGCCT 
GTGGTGGGCC AGTGTACCAT CCGCTCCCTG GAGAGCTTCC TGTGTGACCC CTACTCGGCG 
GAGAGTCCAT CCCCACAGGG TGGCCCAGAC GATGTGAGCC TACTCAGTCC TGGGGAAGAC 
GTGCTCATCG ACATTGATGA CAAGGAGCCC CTCATCCCCA TCCAGCTTGC AGACGGTCTG 
TCGAGCTTGG CCCCCACTAA CACGGCTTCT CCTCCATCCA GTCCTCATAA GACCCTCCGG 
GAGGAAGAGT TCATCGATTG GTGGAGCAAA TTCTTTGCCT CCATAGGGGA GAGGGAAAAG 
TGCGGCTCCT ACTTGGAGAA GGATTTTGAC ACCCTGAAGG TCTATGACAC ACAGCTGGAG 
AATGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT GTACCGGGGC 
AAGACGCAGG AGGAGACAGA AGATCCATCT GTCATTGGTG AATTTAAGGG CCTCTTCAAA 
ATTTATCCCC TCCCAGAAGA CCCAGCCATC CCCATGCCCC CAAGACAGTT CCACCAGCTG 
GCCGCCCAGG GACCCCAGGA GTGCTTGGTC CGTATCTACA TTGTCCGAGC ATTTGGCCTG 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATCCCC TGCACGCTGG AGCCCGTATT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTAA AGATCACTCT CTATGACTAT 
GACCTCCTCT CCAAGGACGA AAAGATCGGT GAGACGGTCA TCGACCTGGA AAACAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGTGGACTC CCACAGACCT ACTGTGTCTC TGGACCGAAC 
CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG CTCCTCCACC TCTTCTGCCA GCAGCATAGA 
GTCAAGGCAC CTGTGTACCG GACAGACCGT GTAATGTTTC AGGATAAAGA ATATTCCATT 
GAAGAGATAG AGGCTGGCAG GATCCCAAAC CCACACCTGG GCCCAGTGGA GGAGCGTCTG 
GCTCTGCATG TGCTTCAGCA GCAGGGCCTG GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC 
TACAGCCCCC TGCAGCCAGA CATCGAGCAG GGGAAGCTGC AGATGTGGGT CGACCTATTT 
CCGAAGGCCC TGGGGCGGCC TGGACCTCCC TTCAACATCA CCCCACGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATTAT CTGGAATACC AGAGATGTGA TCCTGGATGA CCTGAGCCTC 
ACGGGGGAGA AGATGAGCGA CATTTATGTG AAAGGTTGGA TGATTGGCTT TGAAGAACAC 
AAGCAAAAGA CAGACGTGCA TTATCGTTCC CTGGGAGGTG AAGGCAACTT CAACTGGAGG 
TTCATTTTCC CCTTCGACTA CCTGCCAGCT GAGCAAGTCT GTACCATTGC CAAGAAGGAT 
GCCTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT GTTCCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGATGAT TTTCTGGGCT CCCTGCAGCT CGATCTCAAC 
CGCATGCCCA AGCCAGCCAA GACAGCCGAG AAGTGCTCCT TGGACCAGCT GGATGATGCT 
TTCCACCCAG AATGGTTTGT GTCCCTTTTT GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC 
TGTGTAGCAG AAGAGGGTGA GAAGAAAATA CTGGCGGGCA AGCTGGAAAT GACCTTGGAG 
ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC 
ATGAACCCTA AGCTTGAGGA CCCAAGGCGC CCCGACACCT CCTTCCTGTG GTTTACCTCC 
CCATACAAGA CCATGAAGTT CATCCTGTGG CGGCGTTTCC GGTGGGCCAT CATCCTCTTC 
ATCATCCTCT TCATCCTGCT GCTGTTCCTG GCCATCTTCA TCTACGCCTT CCCGAACTAT 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948747.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804236.1 Pan troglodytes dysferlin (DYSF), transcript variant X19, mRNA.

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CloneID	OPc79286
Clone ID Related Accession (Same CDS sequence)	XM_016948747.1
Accession Version	XM_016948747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGCTGTGTG CTCCTGCCTT CTTCCTGCAG GGCCTTCTCC AAACGTCTGA GGACACAGAC 
CTGCCCTACC CACCACCCCA GAGGGAGGCC AACATCTACA TGGTTCCTCA GAACATCAAG 
CCAGCGCTCC AGCGTACCGC CATTGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT 
TACCAGCTGG CCAACATCTC CTCCCCCAGC CTCGTGGTAG AGTGTCGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TTGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGCCCCC CCATCACCGT CAAGGTCATC 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCCTA CTCGGCGGAG AGTCCATCCC CACAGGGTGG CCCAGACGAT 
GTGAGCCTAC TCAGTCCTGG GGAAGACGTG CTCATCGACA TTGATGACAA GGAGCCCCTC 
ATCCCCATCC AGCTTGCAGA CGGTCTGTCG AGCTTGGCCC CCACTAACAC GGCTTCTCCT 
CCATCCAGTC CTCATAAGAC CCTCCGGGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCATCG ACCTGGAAAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804236.1
CDS	893..7252
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKTLREEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948747.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948747.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGCTGTGTG CTCCTGCCTT CTTCCTGCAG GGCCTTCTCC AAACGTCTGA GGACACAGAC 
CTGCCCTACC CACCACCCCA GAGGGAGGCC AACATCTACA TGGTTCCTCA GAACATCAAG 
CCAGCGCTCC AGCGTACCGC CATTGAGATC CTGGCATGGG GCCTGCGGAA CATGAAGAGT 
TACCAGCTGG CCAACATCTC CTCCCCCAGC CTCGTGGTAG AGTGTCGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TTGACATCTG CACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGCCCCC CCATCACCGT CAAGGTCATC 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCCTA CTCGGCGGAG AGTCCATCCC CACAGGGTGG CCCAGACGAT 
GTGAGCCTAC TCAGTCCTGG GGAAGACGTG CTCATCGACA TTGATGACAA GGAGCCCCTC 
ATCCCCATCC AGCTTGCAGA CGGTCTGTCG AGCTTGGCCC CCACTAACAC GGCTTCTCCT 
CCATCCAGTC CTCATAAGAC CCTCCGGGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG 
ACGGTCATCG ACCTGGAAAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA 
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG 
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948748.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804237.1 Pan troglodytes dysferlin (DYSF), transcript variant X20, mRNA.

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CloneID	OPc79287
Clone ID Related Accession (Same CDS sequence)	XM_016948748.1
Accession Version	XM_016948748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAT 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGTC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TCCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACCA AGTTGGCCCT GGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGAGAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA AGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCC CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCATCGACC TGGAAAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804237.1
CDS	892..7248
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948748.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948748.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAT 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGTC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TCCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACCA AGTTGGCCCT GGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGAGAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA AGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCC CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG 
GTCATCGACC TGGAAAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG 
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA 
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948749.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804238.1 Pan troglodytes dysferlin (DYSF), transcript variant X21, mRNA.

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CloneID	OPc79288
Clone ID Related Accession (Same CDS sequence)	XM_016948749.1
Accession Version	XM_016948749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ATGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCG TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC CAAGTTGGCC 
CTGGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGAGA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAAGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TTATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CCCGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC ATCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTCGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCATCGACCT GGAAAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804238.1
CDS	892..7227
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948749.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948749.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT 
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ATGGGTGCCA CTACTACTAC 
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG 
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCG TCACCTGAGC 
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG 
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC 
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGCTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTCCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC CAAGTTGGCC 
CTGGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACGGGC 
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC 
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGAGA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAAGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TTATCTTCTA CGAGATCGAG 
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG 
GAACGGATGC CCCGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC ATCGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATTGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTCGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GTGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC 
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCATCGACCT GGAAAACAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT 
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC 
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT 
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA 
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAT 
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948750.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804239.1 Pan troglodytes dysferlin (DYSF), transcript variant X22, mRNA.

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CloneID	OPc79289
Clone ID Related Accession (Same CDS sequence)	XM_016948750.1
Accession Version	XM_016948750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCATCGACCT GGAAAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804239.1
CDS	892..7197
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948750.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948750.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC 
GGTGAGACGG TCATCGACCT GGAAAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC 
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA 
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948751.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804240.1 Pan troglodytes dysferlin (DYSF), transcript variant X23, mRNA.

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CloneID	OPc79290
Clone ID Related Accession (Same CDS sequence)	XM_016948751.1
Accession Version	XM_016948751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAT 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGTC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TCCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACCA AGTTGGCCCT GGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGAGAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA AGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCC CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804240.1
CDS	892..7185
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPTDTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948751.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948751.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC 
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TCATCACGGT GGTAGACTCT 
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA 
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGACCGCCTG 
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCACCTGGA 
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCCAG ACCCCTACAC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAT 
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG 
CGCACCCGTC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT 
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA 
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GCTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TCCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACGAGACCA AGTTGGCCCT GGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG 
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGAGAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACGATTTCC 
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA AGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC 
ATCTGTCAAC CGAGTCTGGA ACGGATGCCC CGGCTGGCCT GGTTCCCACT GACGAGGGGC 
AGCCAGCCAT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATTGAGATCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTCGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGT 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG 
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA 
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC 
ATCGACCTGG AAAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT 
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC 
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948752.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804241.1 Pan troglodytes dysferlin (DYSF), transcript variant X24, mRNA.

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CloneID	OPc79291
Clone ID Related Accession (Same CDS sequence)	XM_016948752.1
Accession Version	XM_016948752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGATGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCGTCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTCC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACCAAGT TGGCCCTGGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGAGAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAAGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTTATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCCCGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCATCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804241.1
CDS	892..7155
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948752.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948752.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGATGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCGTCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTCC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACCAAGT TGGCCCTGGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGAGAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAAGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTTATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCCCGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCATCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAGGACG AAAAGATCGG TGAGACGGTC ATCGACCTGG AAAACAGGCT GCTGTCCAAG 
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA 
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC 
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA 
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA 
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948753.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804242.1 Pan troglodytes dysferlin (DYSF), transcript variant X25, mRNA.

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CloneID	OPc79292
Clone ID Related Accession (Same CDS sequence)	XM_016948753.1
Accession Version	XM_016948753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCA TCGACCTGGA AAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804242.1
CDS	891..7133
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGAVKPSKASDLDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948753.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948753.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC 
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCCAGA CCCCTACACA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA 
GTCTTTGATG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG 
TACCAGCTGC GCACCCGTCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC 
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT CCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACGAGACCAA GTTGGCCCTG GTTGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG 
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG 
GATGAGAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAA 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG 
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCCC GGCTGGCCTG GTTCCCACTG 
ACGAGGGGCA GCCAGCCATC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TTGAGATCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG 
TGTCGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT 
GAGACGGTCA TCGACCTGGA AAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT 
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT 
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_016948754.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016804243.1 Pan troglodytes dysferlin (DYSF), transcript variant X26, mRNA.

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CloneID	OPc79293
Clone ID Related Accession (Same CDS sequence)	XM_016948754.1
Accession Version	XM_016948754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	dysferlin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006469.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGATGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCGTCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTCC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACCAAGT TGGCCCTGGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGAGAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAAGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTTATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCCCGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCATCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCATC GACCTGGAAA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016804243.1
CDS	891..7091
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDSPGELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKDPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDYLGFLPTFGPCYINLYGSPREFTGFPDPYTELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQLTDELIAGCSQPLGDIHETPSATHLDQYLYQLRTRHLSQITEAALALKLGHSELPAALEQAEDWLLRLRALAEEPQNSLPDIVIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFPEGKLSVFAETYENETKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDEKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECRGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDAFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_016948754.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes dysferlin (DYSF), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016948754.1

ORF Insert Sequence:

ATGCTAAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC 
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG 
CCCATCTTCA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC 
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG 
AGTATGTCGA AAATCTCTGC ACCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTACA GCCGTGCAGT CTTTGATGGG TGCCACTACT ACTACCTACC CTGGGGTAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGACACC CTCTGCCACC 
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCGTCACC TGAGCCAAAT CACTGAGGCT 
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTCC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACCAAGT TGGCCCTGGT TGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CGGGCAAGAT CAAGCTACCC 
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA 
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG 
GCCCTGGGCG GCGTGATGGA TGAGAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAAGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTTATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG 
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT 
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCCCGG 
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCATCGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATT 
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC 
CCCAGCCTCG TGGTAGAGTG TCGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC 
AAGGACGAAA AGATCGGTGA GACGGTCATC GACCTGGAAA ACAGGCTGCT GTCCAAGTTT 
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT 
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG 
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA 
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG 
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
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オンラインフォーム

オンライン注文

DYSF cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: