TSC2 cDNA ORF clone, Gallus gallus(chicken)

The following TSC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa42372 XM_015294446.2
Latest version!
Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42373 XM_015294447.2
Latest version!
Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42374 XM_015294448.2
Latest version!
Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42375 XM_015294449.2
Latest version!
Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42376 XM_015294450.2
Latest version!
Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42377 XM_015294451.2
Latest version!
Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42378 XM_015294452.2
Latest version!
Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
OGa42376 XM_015294450.1 Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42372 XM_015294446.1 Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42377 XM_015294451.1 Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42373 XM_015294447.1 Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42375 XM_015294449.1 Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42374 XM_015294448.1 Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa42378 XM_015294452.1 Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa42372
    Clone ID Related Accession (Same CDS sequence) XM_015294446.1 , XM_015294446.2
    Accession Version XM_015294446.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tuberin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TAGTTTAAGA CTAGCCAAAA ATGCAAAGCA AGGCTTGAAT
    AACTCTCCTC CAGTGAAAGA GCTGAAAGAA CCCTCTGCAG TTGATGCCTT CCGATCCCGC
    AGCATCAGTG TGTCTGAACA TGTGGTCCGC AGTCGGATAC AGACATCCAT CACTAGTTCC
    AGCTTGGGCT CTGCAGATGA AAATTCAATG GCTCAGGCTG ATGACAACTT AAAAAATCTC
    CATCTGGAAC TCACTGAGAC TTGTCTGGAT ATGATGGCCC GATACGTCTT TTCCAACTTC
    ACTGCGGTGC CTAAGAGGTC TCCTGTGGGT GAGTTTCTGT TGGCTGGAGG AAGGACAAAG
    ACATGGCTGG TTGGCAACAA GTTGGTGACC ATCACTACAA GTGTCGGAAC AGGGACAAGG
    TCCTTGCTTG GCCTGGACTC TGGAGATCTC CAAAGCAGCA CAGAATCAGG CTCAGACCCG
    GTTTTGCAAG TGAGACAAAC TAAAGAAGCT CCAGCAAAGC TCGAATCTCA AGCTGGGCAG
    CAGGTTTGCA GAAGCTCTCG CAACAGAGTC AGATCCATGT CTGGTGGTCA TGCCTTACGT
    GTTGGTGCCT TAGACAGCAC AGCCTCCCAC TTCCCTGGTG GCTCGGCTTC ACAAGGGACA
    CAGAGTCCTC CTGCTCCTGC ACCTCGGTCA GAGAAGACTA ATCCTGCTCC ACAGACACCT
    CTGCAGAAAG AAAAAGCGAA CTTAGCTGCA TATGTCCCAC TGCTGACACA GGGCTGGGCA
    GAAATCCTTG TGCGCAGACC CACAGGTAAC ACAAGCTGGC TAATGAGTCT GGAGAACCCG
    CTCAGTCCCT TTTCTTCAGA CATTAACAAT ATGCCCCTGC AAGAGCTGTC CAACGCACTC
    ATGGCTGCAG AGCGTTTTAA AGAGCACCGA GAAACAGCTC TCTACAAATC CCTGTCTGTC
    CCTTCCTCCA GCTTGGCCAC TGGGACAGCC AAGCCTTCAC TTCTCCAGCG TTCCAACACA
    GTGGCCTCTT TCTCTTCCAT GTACCAGTCC AGTTGTCAAG GGAAGTTGCA CAGGAGCATA
    TCCTGGGCAG AGTCTGCGGT CGTGCTGGAG GAAGGGAGCC CCTTGGAAAT GGAGCTGAAG
    GAAACTGAGT CCCCAGCTGA GTCTCCAGAG AGTGAAGATT TTGAGACGTC GTGTTCTGAG
    CAAGTCCTGA GCGAGGAAAG GTTTGGTAAA GCCCAGGAGT TTTACAGCAG GTCGTCCTCG
    ACCTCTAGCC AAGATGAAAA ATCTCTGAAG TCGGACGAGC TGGTGGAGGG CGGTATTCCC
    ATCGGGAGAG TGCTGCCAGC AGAGGATGGC CGGGCACTGG AGGAGCTCTC CTTCCAGCCC
    TCCCAGCCGC TCAGCAAATC GAGCTCTTCC CCCGAGCTGC AGACTTTGCA GGAGATCCTC
    AAGGATGCAA ATGGCAGAGA AGTAACAAGG AGGCTGAGCA CGGAAGTGAA GTCCAAATCT
    CAGTCTGGGA ACCTGGAAGG GGAAGGACTG GGCAGTTGGC TGAACAAGGG TGAGGATGCC
    AGAGCAGCAG GCTCGGGGGG GTTGGATGGC AGTGGCCCCA CCACCTCGCC ACGCTCCCCA
    TCTGGGCACC GGCCCCGGGG GTACACCATC TCTGACTCTG CCCCATCACG GAGAGGGAAG
    AGGATGGAGA GAGATGCCTT CAAGAGCAGA ACAGCAGCCA CCAATGCTGA GAAAGTACCT
    GGAATAAACC CAAGCTTTGT GTTTTTACAA TTGTACCATT CCCCGTTCTT TGGTGATGAA
    AACAACAAGC CCCTCCTGTT GCCAAATGAG ACGTTCGAAA AATCGGTGCA GCTCCTGGAT
    CAAATTCCTT CGTATGACAC CCACAAGATC GCAGTGCTGT ACGTTGGAGA GGGCCAGAGC
    AACAATGAAA TCGCTATTCT GTCCAATGAA CACGGCTCCT ATCGCTACAC CGAGTTCCTG
    ACAGGCTTAG GGAAGCTCAT TGAGCTCAAA GACTGCCAGC CAGATAAAAT CTACCTCGGT
    GGCCTGGACG TGTGTGGGGA GGATGGACAG TTTACCTACT GCTGGCACGA TGACATCATG
    CAAGCTATTT TCCACATTGC TACACTGATG CCCACAAAGG ATTTGGATAA ATATCGCTGT
    GACAAGAAGA GGCACCTTGG GAATGATTTT GTTTCTATTG TTTACAATGA TTCTGGTGAG
    GACTTCAAAC TGGGAACAAT AAAGGGTCAG TTCAATTTTG TGCATGTTAT CATAACCCCA
    CTTGACTATG ACTGCAACCT GGTCACACTG CAGTGTCGAA AAGACATGGA GGGCCTTGTA
    GACACGAGTG TTGCTAAGAT CATCTCTGAC AAGAACCTGC CATTCGTGGC CCGTCAGATG
    GCCCTGCATG CTAACATGGC TTCCCAGGTG CACCACAGTC GATCCAACCC TACTGACACC
    TACCCATCCA AATGGATCGC TCGGCTGCGG CACATCAAAC GGCTGAGGCA CCGGCTACGT
    GAAGAAACCC AGTACCAAAC TCCTGGCCTT CCCCTGCAGA TGCACCCGTC TGCTCCAACA
    AAGCCCTCTC CCCAGATGGC TCAGGACCCA CCGCCGGCCT ACGAGACCGG TCAGAGGAAG
    CGGCTGATCT CCTCGGTGGA TGACTTCACT GAGTTTGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149932.1
    CDS58..5559
    Translation

    Target ORF information:

    RefSeq Version XM_015294446.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TAGTTTAAGA CTAGCCAAAA ATGCAAAGCA AGGCTTGAAT
    AACTCTCCTC CAGTGAAAGA GCTGAAAGAA CCCTCTGCAG TTGATGCCTT CCGATCCCGC
    AGCATCAGTG TGTCTGAACA TGTGGTCCGC AGTCGGATAC AGACATCCAT CACTAGTTCC
    AGCTTGGGCT CTGCAGATGA AAATTCAATG GCTCAGGCTG ATGACAACTT AAAAAATCTC
    CATCTGGAAC TCACTGAGAC TTGTCTGGAT ATGATGGCCC GATACGTCTT TTCCAACTTC
    ACTGCGGTGC CTAAGAGGTC TCCTGTGGGT GAGTTTCTGT TGGCTGGAGG AAGGACAAAG
    ACATGGCTGG TTGGCAACAA GTTGGTGACC ATCACTACAA GTGTCGGAAC AGGGACAAGG
    TCCTTGCTTG GCCTGGACTC TGGAGATCTC CAAAGCAGCA CAGAATCAGG CTCAGACCCG
    GTTTTGCAAG TGAGACAAAC TAAAGAAGCT CCAGCAAAGC TCGAATCTCA AGCTGGGCAG
    CAGGTTTGCA GAAGCTCTCG CAACAGAGTC AGATCCATGT CTGGTGGTCA TGCCTTACGT
    GTTGGTGCCT TAGACAGCAC AGCCTCCCAC TTCCCTGGTG GCTCGGCTTC ACAAGGGACA
    CAGAGTCCTC CTGCTCCTGC ACCTCGGTCA GAGAAGACTA ATCCTGCTCC ACAGACACCT
    CTGCAGAAAG AAAAAGCGAA CTTAGCTGCA TATGTCCCAC TGCTGACACA GGGCTGGGCA
    GAAATCCTTG TGCGCAGACC CACAGGTAAC ACAAGCTGGC TAATGAGTCT GGAGAACCCG
    CTCAGTCCCT TTTCTTCAGA CATTAACAAT ATGCCCCTGC AAGAGCTGTC CAACGCACTC
    ATGGCTGCAG AGCGTTTTAA AGAGCACCGA GAAACAGCTC TCTACAAATC CCTGTCTGTC
    CCTTCCTCCA GCTTGGCCAC TGGGACAGCC AAGCCTTCAC TTCTCCAGCG TTCCAACACA
    GTGGCCTCTT TCTCTTCCAT GTACCAGTCC AGTTGTCAAG GGAAGTTGCA CAGGAGCATA
    TCCTGGGCAG AGTCTGCGGT CGTGCTGGAG GAAGGGAGCC CCTTGGAAAT GGAGCTGAAG
    GAAACTGAGT CCCCAGCTGA GTCTCCAGAG AGTGAAGATT TTGAGACGTC GTGTTCTGAG
    CAAGTCCTGA GCGAGGAAAG GTTTGGTAAA GCCCAGGAGT TTTACAGCAG GTCGTCCTCG
    ACCTCTAGCC AAGATGAAAA ATCTCTGAAG TCGGACGAGC TGGTGGAGGG CGGTATTCCC
    ATCGGGAGAG TGCTGCCAGC AGAGGATGGC CGGGCACTGG AGGAGCTCTC CTTCCAGCCC
    TCCCAGCCGC TCAGCAAATC GAGCTCTTCC CCCGAGCTGC AGACTTTGCA GGAGATCCTC
    AAGGATGCAA ATGGCAGAGA AGTAACAAGG AGGCTGAGCA CGGAAGTGAA GTCCAAATCT
    CAGTCTGGGA ACCTGGAAGG GGAAGGACTG GGCAGTTGGC TGAACAAGGG TGAGGATGCC
    AGAGCAGCAG GCTCGGGGGG GTTGGATGGC AGTGGCCCCA CCACCTCGCC ACGCTCCCCA
    TCTGGGCACC GGCCCCGGGG GTACACCATC TCTGACTCTG CCCCATCACG GAGAGGGAAG
    AGGATGGAGA GAGATGCCTT CAAGAGCAGA ACAGCAGCCA CCAATGCTGA GAAAGTACCT
    GGAATAAACC CAAGCTTTGT GTTTTTACAA TTGTACCATT CCCCGTTCTT TGGTGATGAA
    AACAACAAGC CCCTCCTGTT GCCAAATGAG ACGTTCGAAA AATCGGTGCA GCTCCTGGAT
    CAAATTCCTT CGTATGACAC CCACAAGATC GCAGTGCTGT ACGTTGGAGA GGGCCAGAGC
    AACAATGAAA TCGCTATTCT GTCCAATGAA CACGGCTCCT ATCGCTACAC CGAGTTCCTG
    ACAGGCTTAG GGAAGCTCAT TGAGCTCAAA GACTGCCAGC CAGATAAAAT CTACCTCGGT
    GGCCTGGACG TGTGTGGGGA GGATGGACAG TTTACCTACT GCTGGCACGA TGACATCATG
    CAAGCTATTT TCCACATTGC TACACTGATG CCCACAAAGG ATTTGGATAA ATATCGCTGT
    GACAAGAAGA GGCACCTTGG GAATGATTTT GTTTCTATTG TTTACAATGA TTCTGGTGAG
    GACTTCAAAC TGGGAACAAT AAAGGGTCAG TTCAATTTTG TGCATGTTAT CATAACCCCA
    CTTGACTATG ACTGCAACCT GGTCACACTG CAGTGTCGAA AAGACATGGA GGGCCTTGTA
    GACACGAGTG TTGCTAAGAT CATCTCTGAC AAGAACCTGC CATTCGTGGC CCGTCAGATG
    GCCCTGCATG CTAACATGGC TTCCCAGGTG CACCACAGTC GATCCAACCC TACTGACACC
    TACCCATCCA AATGGATCGC TCGGCTGCGG CACATCAAAC GGCTGAGGCA CCGGCTACGT
    GAAGAAACCC AGTACCAAAC TCCTGGCCTT CCCCTGCAGA TGCACCCGTC TGCTCCAACA
    AAGCCCTCTC CCCAGATGGC TCAGGACCCA CCGCCGGCCT ACGAGACCGG TCAGAGGAAG
    CGGCTGATCT CCTCGGTGGA TGACTTCACT GAGTTTGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42372
    Clone ID Related Accession (Same CDS sequence) XM_015294446.1 , XM_015294446.2
    Accession Version XM_015294446.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tuberin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TAGTTTAAGA CTAGCCAAAA ATGCAAAGCA AGGCTTGAAT
    AACTCTCCTC CAGTGAAAGA GCTGAAAGAA CCCTCTGCAG TTGATGCCTT CCGATCCCGC
    AGCATCAGTG TGTCTGAACA TGTGGTCCGC AGTCGGATAC AGACATCCAT CACTAGTTCC
    AGCTTGGGCT CTGCAGATGA AAATTCAATG GCTCAGGCTG ATGACAACTT AAAAAATCTC
    CATCTGGAAC TCACTGAGAC TTGTCTGGAT ATGATGGCCC GATACGTCTT TTCCAACTTC
    ACTGCGGTGC CTAAGAGGTC TCCTGTGGGT GAGTTTCTGT TGGCTGGAGG AAGGACAAAG
    ACATGGCTGG TTGGCAACAA GTTGGTGACC ATCACTACAA GTGTCGGAAC AGGGACAAGG
    TCCTTGCTTG GCCTGGACTC TGGAGATCTC CAAAGCAGCA CAGAATCAGG CTCAGACCCG
    GTTTTGCAAG TGAGACAAAC TAAAGAAGCT CCAGCAAAGC TCGAATCTCA AGCTGGGCAG
    CAGGTTTGCA GAAGCTCTCG CAACAGAGTC AGATCCATGT CTGGTGGTCA TGCCTTACGT
    GTTGGTGCCT TAGACAGCAC AGCCTCCCAC TTCCCTGGTG GCTCGGCTTC ACAAGGGACA
    CAGAGTCCTC CTGCTCCTGC ACCTCGGTCA GAGAAGACTA ATCCTGCTCC ACAGACACCT
    CTGCAGAAAG AAAAAGCGAA CTTAGCTGCA TATGTCCCAC TGCTGACACA GGGCTGGGCA
    GAAATCCTTG TGCGCAGACC CACAGGTAAC ACAAGCTGGC TAATGAGTCT GGAGAACCCG
    CTCAGTCCCT TTTCTTCAGA CATTAACAAT ATGCCCCTGC AAGAGCTGTC CAACGCACTC
    ATGGCTGCAG AGCGTTTTAA AGAGCACCGA GAAACAGCTC TCTACAAATC CCTGTCTGTC
    CCTTCCTCCA GCTTGGCCAC TGGGACAGCC AAGCCTTCAC TTCTCCAGCG TTCCAACACA
    GTGGCCTCTT TCTCTTCCAT GTACCAGTCC AGTTGTCAAG GGAAGTTGCA CAGGAGCATA
    TCCTGGGCAG AGTCTGCGGT CGTGCTGGAG GAAGGGAGCC CCTTGGAAAT GGAGCTGAAG
    GAAACTGAGT CCCCAGCTGA GTCTCCAGAG AGTGAAGATT TTGAGACGTC GTGTTCTGAG
    CAAGTCCTGA GCGAGGAAAG GTTTGGTAAA GCCCAGGAGT TTTACAGCAG GTCGTCCTCG
    ACCTCTAGCC AAGATGAAAA ATCTCTGAAG TCGGACGAGC TGGTGGAGGG CGGTATTCCC
    ATCGGGAGAG TGCTGCCAGC AGAGGATGGC CGGGCACTGG AGGAGCTCTC CTTCCAGCCC
    TCCCAGCCGC TCAGCAAATC GAGCTCTTCC CCCGAGCTGC AGACTTTGCA GGAGATCCTC
    AAGGATGCAA ATGGCAGAGA AGTAACAAGG AGGCTGAGCA CGGAAGTGAA GTCCAAATCT
    CAGTCTGGGA ACCTGGAAGG GGAAGGACTG GGCAGTTGGC TGAACAAGGG TGAGGATGCC
    AGAGCAGCAG GCTCGGGGGG GTTGGATGGC AGTGGCCCCA CCACCTCGCC ACGCTCCCCA
    TCTGGGCACC GGCCCCGGGG GTACACCATC TCTGACTCTG CCCCATCACG GAGAGGGAAG
    AGGATGGAGA GAGATGCCTT CAAGAGCAGA ACAGCAGCCA CCAATGCTGA GAAAGTACCT
    GGAATAAACC CAAGCTTTGT GTTTTTACAA TTGTACCATT CCCCGTTCTT TGGTGATGAA
    AACAACAAGC CCCTCCTGTT GCCAAATGAG ACGTTCGAAA AATCGGTGCA GCTCCTGGAT
    CAAATTCCTT CGTATGACAC CCACAAGATC GCAGTGCTGT ACGTTGGAGA GGGCCAGAGC
    AACAATGAAA TCGCTATTCT GTCCAATGAA CACGGCTCCT ATCGCTACAC CGAGTTCCTG
    ACAGGCTTAG GGAAGCTCAT TGAGCTCAAA GACTGCCAGC CAGATAAAAT CTACCTCGGT
    GGCCTGGACG TGTGTGGGGA GGATGGACAG TTTACCTACT GCTGGCACGA TGACATCATG
    CAAGCTATTT TCCACATTGC TACACTGATG CCCACAAAGG ATTTGGATAA ATATCGCTGT
    GACAAGAAGA GGCACCTTGG GAATGATTTT GTTTCTATTG TTTACAATGA TTCTGGTGAG
    GACTTCAAAC TGGGAACAAT AAAGGGTCAG TTCAATTTTG TGCATGTTAT CATAACCCCA
    CTTGACTATG ACTGCAACCT GGTCACACTG CAGTGTCGAA AAGACATGGA GGGCCTTGTA
    GACACGAGTG TTGCTAAGAT CATCTCTGAC AAGAACCTGC CATTCGTGGC CCGTCAGATG
    GCCCTGCATG CTAACATGGC TTCCCAGGTG CACCACAGTC GATCCAACCC TACTGACACC
    TACCCATCCA AATGGATCGC TCGGCTGCGG CACATCAAAC GGCTGAGGCA CCGGCTACGT
    GAAGAAACCC AGTACCAAAC TCCTGGCCTT CCCCTGCAGA TGCACCCGTC TGCTCCAACA
    AAGCCCTCTC CCCAGATGGC TCAGGACCCA CCGCCGGCCT ACGAGACCGG TCAGAGGAAG
    CGGCTGATCT CCTCGGTGGA TGACTTCACT GAGTTTGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149932.1
    CDS59..5560
    Translation

    Target ORF information:

    RefSeq Version XM_015294446.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294446.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TAGTTTAAGA CTAGCCAAAA ATGCAAAGCA AGGCTTGAAT
    AACTCTCCTC CAGTGAAAGA GCTGAAAGAA CCCTCTGCAG TTGATGCCTT CCGATCCCGC
    AGCATCAGTG TGTCTGAACA TGTGGTCCGC AGTCGGATAC AGACATCCAT CACTAGTTCC
    AGCTTGGGCT CTGCAGATGA AAATTCAATG GCTCAGGCTG ATGACAACTT AAAAAATCTC
    CATCTGGAAC TCACTGAGAC TTGTCTGGAT ATGATGGCCC GATACGTCTT TTCCAACTTC
    ACTGCGGTGC CTAAGAGGTC TCCTGTGGGT GAGTTTCTGT TGGCTGGAGG AAGGACAAAG
    ACATGGCTGG TTGGCAACAA GTTGGTGACC ATCACTACAA GTGTCGGAAC AGGGACAAGG
    TCCTTGCTTG GCCTGGACTC TGGAGATCTC CAAAGCAGCA CAGAATCAGG CTCAGACCCG
    GTTTTGCAAG TGAGACAAAC TAAAGAAGCT CCAGCAAAGC TCGAATCTCA AGCTGGGCAG
    CAGGTTTGCA GAAGCTCTCG CAACAGAGTC AGATCCATGT CTGGTGGTCA TGCCTTACGT
    GTTGGTGCCT TAGACAGCAC AGCCTCCCAC TTCCCTGGTG GCTCGGCTTC ACAAGGGACA
    CAGAGTCCTC CTGCTCCTGC ACCTCGGTCA GAGAAGACTA ATCCTGCTCC ACAGACACCT
    CTGCAGAAAG AAAAAGCGAA CTTAGCTGCA TATGTCCCAC TGCTGACACA GGGCTGGGCA
    GAAATCCTTG TGCGCAGACC CACAGGTAAC ACAAGCTGGC TAATGAGTCT GGAGAACCCG
    CTCAGTCCCT TTTCTTCAGA CATTAACAAT ATGCCCCTGC AAGAGCTGTC CAACGCACTC
    ATGGCTGCAG AGCGTTTTAA AGAGCACCGA GAAACAGCTC TCTACAAATC CCTGTCTGTC
    CCTTCCTCCA GCTTGGCCAC TGGGACAGCC AAGCCTTCAC TTCTCCAGCG TTCCAACACA
    GTGGCCTCTT TCTCTTCCAT GTACCAGTCC AGTTGTCAAG GGAAGTTGCA CAGGAGCATA
    TCCTGGGCAG AGTCTGCGGT CGTGCTGGAG GAAGGGAGCC CCTTGGAAAT GGAGCTGAAG
    GAAACTGAGT CCCCAGCTGA GTCTCCAGAG AGTGAAGATT TTGAGACGTC GTGTTCTGAG
    CAAGTCCTGA GCGAGGAAAG GTTTGGTAAA GCCCAGGAGT TTTACAGCAG GTCGTCCTCG
    ACCTCTAGCC AAGATGAAAA ATCTCTGAAG TCGGACGAGC TGGTGGAGGG CGGTATTCCC
    ATCGGGAGAG TGCTGCCAGC AGAGGATGGC CGGGCACTGG AGGAGCTCTC CTTCCAGCCC
    TCCCAGCCGC TCAGCAAATC GAGCTCTTCC CCCGAGCTGC AGACTTTGCA GGAGATCCTC
    AAGGATGCAA ATGGCAGAGA AGTAACAAGG AGGCTGAGCA CGGAAGTGAA GTCCAAATCT
    CAGTCTGGGA ACCTGGAAGG GGAAGGACTG GGCAGTTGGC TGAACAAGGG TGAGGATGCC
    AGAGCAGCAG GCTCGGGGGG GTTGGATGGC AGTGGCCCCA CCACCTCGCC ACGCTCCCCA
    TCTGGGCACC GGCCCCGGGG GTACACCATC TCTGACTCTG CCCCATCACG GAGAGGGAAG
    AGGATGGAGA GAGATGCCTT CAAGAGCAGA ACAGCAGCCA CCAATGCTGA GAAAGTACCT
    GGAATAAACC CAAGCTTTGT GTTTTTACAA TTGTACCATT CCCCGTTCTT TGGTGATGAA
    AACAACAAGC CCCTCCTGTT GCCAAATGAG ACGTTCGAAA AATCGGTGCA GCTCCTGGAT
    CAAATTCCTT CGTATGACAC CCACAAGATC GCAGTGCTGT ACGTTGGAGA GGGCCAGAGC
    AACAATGAAA TCGCTATTCT GTCCAATGAA CACGGCTCCT ATCGCTACAC CGAGTTCCTG
    ACAGGCTTAG GGAAGCTCAT TGAGCTCAAA GACTGCCAGC CAGATAAAAT CTACCTCGGT
    GGCCTGGACG TGTGTGGGGA GGATGGACAG TTTACCTACT GCTGGCACGA TGACATCATG
    CAAGCTATTT TCCACATTGC TACACTGATG CCCACAAAGG ATTTGGATAA ATATCGCTGT
    GACAAGAAGA GGCACCTTGG GAATGATTTT GTTTCTATTG TTTACAATGA TTCTGGTGAG
    GACTTCAAAC TGGGAACAAT AAAGGGTCAG TTCAATTTTG TGCATGTTAT CATAACCCCA
    CTTGACTATG ACTGCAACCT GGTCACACTG CAGTGTCGAA AAGACATGGA GGGCCTTGTA
    GACACGAGTG TTGCTAAGAT CATCTCTGAC AAGAACCTGC CATTCGTGGC CCGTCAGATG
    GCCCTGCATG CTAACATGGC TTCCCAGGTG CACCACAGTC GATCCAACCC TACTGACACC
    TACCCATCCA AATGGATCGC TCGGCTGCGG CACATCAAAC GGCTGAGGCA CCGGCTACGT
    GAAGAAACCC AGTACCAAAC TCCTGGCCTT CCCCTGCAGA TGCACCCGTC TGCTCCAACA
    AAGCCCTCTC CCCAGATGGC TCAGGACCCA CCGCCGGCCT ACGAGACCGG TCAGAGGAAG
    CGGCTGATCT CCTCGGTGGA TGACTTCACT GAGTTTGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42373
    Clone ID Related Accession (Same CDS sequence) XM_015294447.2 , XM_015294447.1
    Accession Version XM_015294447.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tuberin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TTTAAGACTA GCCAAAAATG CAAAGCAAGG CTTGAATAAC
    TCTCCTCCAG TGAAAGAGCT GAAAGAACCC TCTGCAGTTG ATGCCTTCCG ATCCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGT CGGATACAGA CATCCATCAC TAGTTCCAGC
    TTGGGCTCTG CAGATGAAAA TTCAATGGCT CAGGCTGATG ACAACTTAAA AAATCTCCAT
    CTGGAACTCA CTGAGACTTG TCTGGATATG ATGGCCCGAT ACGTCTTTTC CAACTTCACT
    GCGGTGCCTA AGAGGTCTCC TGTGGGTGAG TTTCTGTTGG CTGGAGGAAG GACAAAGACA
    TGGCTGGTTG GCAACAAGTT GGTGACCATC ACTACAAGTG TCGGAACAGG GACAAGGTCC
    TTGCTTGGCC TGGACTCTGG AGATCTCCAA AGCAGCACAG AATCAGGCTC AGACCCGGTT
    TTGCAAGTGA GACAAACTAA AGAAGCTCCA GCAAAGCTCG AATCTCAAGC TGGGCAGCAG
    GTTTGCAGAA GCTCTCGCAA CAGAGTCAGA TCCATGTCTG GTGGTCATGC CTTACGTGTT
    GGTGCCTTAG ACAGCACAGC CTCCCACTTC CCTGGTGGCT CGGCTTCACA AGGGACACAG
    AGTCCTCCTG CTCCTGCACC TCGGTCAGAG AAGACTAATC CTGCTCCACA GACACCTCTG
    CAGAAAGAAA AAGCGAACTT AGCTGCATAT GTCCCACTGC TGACACAGGG CTGGGCAGAA
    ATCCTTGTGC GCAGACCCAC AGGTAACACA AGCTGGCTAA TGAGTCTGGA GAACCCGCTC
    AGTCCCTTTT CTTCAGACAT TAACAATATG CCCCTGCAAG AGCTGTCCAA CGCACTCATG
    GCTGCAGAGC GTTTTAAAGA GCACCGAGAA ACAGCTCTCT ACAAATCCCT GTCTGTCCCT
    TCCTCCAGCT TGGCCACTGG GACAGCCAAG CCTTCACTTC TCCAGCGTTC CAACACAGTG
    GCCTCTTTCT CTTCCATGTA CCAGTCCAGT TGTCAAGGGA AGTTGCACAG GAGCATATCC
    TGGGCAGAGT CTGCGGTCGT GCTGGAGGAA GGGAGCCCCT TGGAAATGGA GCTGAAGGAA
    ACTGAGTCCC CAGCTGAGTC TCCAGAGAGT GAAGATTTTG AGACGTCGTG TTCTGAGCAA
    GTCCTGAGCG AGGAAAGGTT TGGTAAAGCC CAGGAGTTTT ACAGCAGGTC GTCCTCGACC
    TCTAGCCAAG ATGAAAAATC TCTGAAGTCG GACGAGCTGG TGGAGGGCGG TATTCCCATC
    GGGAGAGTGC TGCCAGCAGA GGATGGCCGG GCACTGGAGG AGCTCTCCTT CCAGCCCTCC
    CAGCCGCTCA GCAAATCGAG CTCTTCCCCC GAGCTGCAGA CTTTGCAGGA GATCCTCAAG
    GATGCAAATG GCAGAGAAGT AACAAGGAGG CTGAGCACGG AAGTGAAGTC CAAATCTCAG
    TCTGGGAACC TGGAAGGGGA AGGACTGGGC AGTTGGCTGA ACAAGGGTGA GGATGCCAGA
    GCAGCAGGCT CGGGGGGGTT GGATGGCAGT GGCCCCACCA CCTCGCCACG CTCCCCATCT
    GGGCACCGGC CCCGGGGGTA CACCATCTCT GACTCTGCCC CATCACGGAG AGGGAAGAGG
    ATGGAGAGAG ATGCCTTCAA GAGCAGAACA GCAGCCACCA ATGCTGAGAA AGTACCTGGA
    ATAAACCCAA GCTTTGTGTT TTTACAATTG TACCATTCCC CGTTCTTTGG TGATGAAAAC
    AACAAGCCCC TCCTGTTGCC AAATGAGACG TTCGAAAAAT CGGTGCAGCT CCTGGATCAA
    ATTCCTTCGT ATGACACCCA CAAGATCGCA GTGCTGTACG TTGGAGAGGG CCAGAGCAAC
    AATGAAATCG CTATTCTGTC CAATGAACAC GGCTCCTATC GCTACACCGA GTTCCTGACA
    GGCTTAGGGA AGCTCATTGA GCTCAAAGAC TGCCAGCCAG ATAAAATCTA CCTCGGTGGC
    CTGGACGTGT GTGGGGAGGA TGGACAGTTT ACCTACTGCT GGCACGATGA CATCATGCAA
    GCTATTTTCC ACATTGCTAC ACTGATGCCC ACAAAGGATT TGGATAAATA TCGCTGTGAC
    AAGAAGAGGC ACCTTGGGAA TGATTTTGTT TCTATTGTTT ACAATGATTC TGGTGAGGAC
    TTCAAACTGG GAACAATAAA GGGTCAGTTC AATTTTGTGC ATGTTATCAT AACCCCACTT
    GACTATGACT GCAACCTGGT CACACTGCAG TGTCGAAAAG ACATGGAGGG CCTTGTAGAC
    ACGAGTGTTG CTAAGATCAT CTCTGACAAG AACCTGCCAT TCGTGGCCCG TCAGATGGCC
    CTGCATGCTA ACATGGCTTC CCAGGTGCAC CACAGTCGAT CCAACCCTAC TGACACCTAC
    CCATCCAAAT GGATCGCTCG GCTGCGGCAC ATCAAACGGC TGAGGCACCG GCTACGTGAA
    GAAACCCAGT ACCAAACTCC TGGCCTTCCC CTGCAGATGC ACCCGTCTGC TCCAACAAAG
    CCCTCTCCCC AGATGGCTCA GGACCCACCG CCGGCCTACG AGACCGGTCA GAGGAAGCGG
    CTGATCTCCT CGGTGGATGA CTTCACTGAG TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149933.1
    CDS59..5557
    Translation

    Target ORF information:

    RefSeq Version XM_015294447.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294447.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TTTAAGACTA GCCAAAAATG CAAAGCAAGG CTTGAATAAC
    TCTCCTCCAG TGAAAGAGCT GAAAGAACCC TCTGCAGTTG ATGCCTTCCG ATCCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGT CGGATACAGA CATCCATCAC TAGTTCCAGC
    TTGGGCTCTG CAGATGAAAA TTCAATGGCT CAGGCTGATG ACAACTTAAA AAATCTCCAT
    CTGGAACTCA CTGAGACTTG TCTGGATATG ATGGCCCGAT ACGTCTTTTC CAACTTCACT
    GCGGTGCCTA AGAGGTCTCC TGTGGGTGAG TTTCTGTTGG CTGGAGGAAG GACAAAGACA
    TGGCTGGTTG GCAACAAGTT GGTGACCATC ACTACAAGTG TCGGAACAGG GACAAGGTCC
    TTGCTTGGCC TGGACTCTGG AGATCTCCAA AGCAGCACAG AATCAGGCTC AGACCCGGTT
    TTGCAAGTGA GACAAACTAA AGAAGCTCCA GCAAAGCTCG AATCTCAAGC TGGGCAGCAG
    GTTTGCAGAA GCTCTCGCAA CAGAGTCAGA TCCATGTCTG GTGGTCATGC CTTACGTGTT
    GGTGCCTTAG ACAGCACAGC CTCCCACTTC CCTGGTGGCT CGGCTTCACA AGGGACACAG
    AGTCCTCCTG CTCCTGCACC TCGGTCAGAG AAGACTAATC CTGCTCCACA GACACCTCTG
    CAGAAAGAAA AAGCGAACTT AGCTGCATAT GTCCCACTGC TGACACAGGG CTGGGCAGAA
    ATCCTTGTGC GCAGACCCAC AGGTAACACA AGCTGGCTAA TGAGTCTGGA GAACCCGCTC
    AGTCCCTTTT CTTCAGACAT TAACAATATG CCCCTGCAAG AGCTGTCCAA CGCACTCATG
    GCTGCAGAGC GTTTTAAAGA GCACCGAGAA ACAGCTCTCT ACAAATCCCT GTCTGTCCCT
    TCCTCCAGCT TGGCCACTGG GACAGCCAAG CCTTCACTTC TCCAGCGTTC CAACACAGTG
    GCCTCTTTCT CTTCCATGTA CCAGTCCAGT TGTCAAGGGA AGTTGCACAG GAGCATATCC
    TGGGCAGAGT CTGCGGTCGT GCTGGAGGAA GGGAGCCCCT TGGAAATGGA GCTGAAGGAA
    ACTGAGTCCC CAGCTGAGTC TCCAGAGAGT GAAGATTTTG AGACGTCGTG TTCTGAGCAA
    GTCCTGAGCG AGGAAAGGTT TGGTAAAGCC CAGGAGTTTT ACAGCAGGTC GTCCTCGACC
    TCTAGCCAAG ATGAAAAATC TCTGAAGTCG GACGAGCTGG TGGAGGGCGG TATTCCCATC
    GGGAGAGTGC TGCCAGCAGA GGATGGCCGG GCACTGGAGG AGCTCTCCTT CCAGCCCTCC
    CAGCCGCTCA GCAAATCGAG CTCTTCCCCC GAGCTGCAGA CTTTGCAGGA GATCCTCAAG
    GATGCAAATG GCAGAGAAGT AACAAGGAGG CTGAGCACGG AAGTGAAGTC CAAATCTCAG
    TCTGGGAACC TGGAAGGGGA AGGACTGGGC AGTTGGCTGA ACAAGGGTGA GGATGCCAGA
    GCAGCAGGCT CGGGGGGGTT GGATGGCAGT GGCCCCACCA CCTCGCCACG CTCCCCATCT
    GGGCACCGGC CCCGGGGGTA CACCATCTCT GACTCTGCCC CATCACGGAG AGGGAAGAGG
    ATGGAGAGAG ATGCCTTCAA GAGCAGAACA GCAGCCACCA ATGCTGAGAA AGTACCTGGA
    ATAAACCCAA GCTTTGTGTT TTTACAATTG TACCATTCCC CGTTCTTTGG TGATGAAAAC
    AACAAGCCCC TCCTGTTGCC AAATGAGACG TTCGAAAAAT CGGTGCAGCT CCTGGATCAA
    ATTCCTTCGT ATGACACCCA CAAGATCGCA GTGCTGTACG TTGGAGAGGG CCAGAGCAAC
    AATGAAATCG CTATTCTGTC CAATGAACAC GGCTCCTATC GCTACACCGA GTTCCTGACA
    GGCTTAGGGA AGCTCATTGA GCTCAAAGAC TGCCAGCCAG ATAAAATCTA CCTCGGTGGC
    CTGGACGTGT GTGGGGAGGA TGGACAGTTT ACCTACTGCT GGCACGATGA CATCATGCAA
    GCTATTTTCC ACATTGCTAC ACTGATGCCC ACAAAGGATT TGGATAAATA TCGCTGTGAC
    AAGAAGAGGC ACCTTGGGAA TGATTTTGTT TCTATTGTTT ACAATGATTC TGGTGAGGAC
    TTCAAACTGG GAACAATAAA GGGTCAGTTC AATTTTGTGC ATGTTATCAT AACCCCACTT
    GACTATGACT GCAACCTGGT CACACTGCAG TGTCGAAAAG ACATGGAGGG CCTTGTAGAC
    ACGAGTGTTG CTAAGATCAT CTCTGACAAG AACCTGCCAT TCGTGGCCCG TCAGATGGCC
    CTGCATGCTA ACATGGCTTC CCAGGTGCAC CACAGTCGAT CCAACCCTAC TGACACCTAC
    CCATCCAAAT GGATCGCTCG GCTGCGGCAC ATCAAACGGC TGAGGCACCG GCTACGTGAA
    GAAACCCAGT ACCAAACTCC TGGCCTTCCC CTGCAGATGC ACCCGTCTGC TCCAACAAAG
    CCCTCTCCCC AGATGGCTCA GGACCCACCG CCGGCCTACG AGACCGGTCA GAGGAAGCGG
    CTGATCTCCT CGGTGGATGA CTTCACTGAG TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42373
    Clone ID Related Accession (Same CDS sequence) XM_015294447.2 , XM_015294447.1
    Accession Version XM_015294447.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tuberin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TTTAAGACTA GCCAAAAATG CAAAGCAAGG CTTGAATAAC
    TCTCCTCCAG TGAAAGAGCT GAAAGAACCC TCTGCAGTTG ATGCCTTCCG ATCCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGT CGGATACAGA CATCCATCAC TAGTTCCAGC
    TTGGGCTCTG CAGATGAAAA TTCAATGGCT CAGGCTGATG ACAACTTAAA AAATCTCCAT
    CTGGAACTCA CTGAGACTTG TCTGGATATG ATGGCCCGAT ACGTCTTTTC CAACTTCACT
    GCGGTGCCTA AGAGGTCTCC TGTGGGTGAG TTTCTGTTGG CTGGAGGAAG GACAAAGACA
    TGGCTGGTTG GCAACAAGTT GGTGACCATC ACTACAAGTG TCGGAACAGG GACAAGGTCC
    TTGCTTGGCC TGGACTCTGG AGATCTCCAA AGCAGCACAG AATCAGGCTC AGACCCGGTT
    TTGCAAGTGA GACAAACTAA AGAAGCTCCA GCAAAGCTCG AATCTCAAGC TGGGCAGCAG
    GTTTGCAGAA GCTCTCGCAA CAGAGTCAGA TCCATGTCTG GTGGTCATGC CTTACGTGTT
    GGTGCCTTAG ACAGCACAGC CTCCCACTTC CCTGGTGGCT CGGCTTCACA AGGGACACAG
    AGTCCTCCTG CTCCTGCACC TCGGTCAGAG AAGACTAATC CTGCTCCACA GACACCTCTG
    CAGAAAGAAA AAGCGAACTT AGCTGCATAT GTCCCACTGC TGACACAGGG CTGGGCAGAA
    ATCCTTGTGC GCAGACCCAC AGGTAACACA AGCTGGCTAA TGAGTCTGGA GAACCCGCTC
    AGTCCCTTTT CTTCAGACAT TAACAATATG CCCCTGCAAG AGCTGTCCAA CGCACTCATG
    GCTGCAGAGC GTTTTAAAGA GCACCGAGAA ACAGCTCTCT ACAAATCCCT GTCTGTCCCT
    TCCTCCAGCT TGGCCACTGG GACAGCCAAG CCTTCACTTC TCCAGCGTTC CAACACAGTG
    GCCTCTTTCT CTTCCATGTA CCAGTCCAGT TGTCAAGGGA AGTTGCACAG GAGCATATCC
    TGGGCAGAGT CTGCGGTCGT GCTGGAGGAA GGGAGCCCCT TGGAAATGGA GCTGAAGGAA
    ACTGAGTCCC CAGCTGAGTC TCCAGAGAGT GAAGATTTTG AGACGTCGTG TTCTGAGCAA
    GTCCTGAGCG AGGAAAGGTT TGGTAAAGCC CAGGAGTTTT ACAGCAGGTC GTCCTCGACC
    TCTAGCCAAG ATGAAAAATC TCTGAAGTCG GACGAGCTGG TGGAGGGCGG TATTCCCATC
    GGGAGAGTGC TGCCAGCAGA GGATGGCCGG GCACTGGAGG AGCTCTCCTT CCAGCCCTCC
    CAGCCGCTCA GCAAATCGAG CTCTTCCCCC GAGCTGCAGA CTTTGCAGGA GATCCTCAAG
    GATGCAAATG GCAGAGAAGT AACAAGGAGG CTGAGCACGG AAGTGAAGTC CAAATCTCAG
    TCTGGGAACC TGGAAGGGGA AGGACTGGGC AGTTGGCTGA ACAAGGGTGA GGATGCCAGA
    GCAGCAGGCT CGGGGGGGTT GGATGGCAGT GGCCCCACCA CCTCGCCACG CTCCCCATCT
    GGGCACCGGC CCCGGGGGTA CACCATCTCT GACTCTGCCC CATCACGGAG AGGGAAGAGG
    ATGGAGAGAG ATGCCTTCAA GAGCAGAACA GCAGCCACCA ATGCTGAGAA AGTACCTGGA
    ATAAACCCAA GCTTTGTGTT TTTACAATTG TACCATTCCC CGTTCTTTGG TGATGAAAAC
    AACAAGCCCC TCCTGTTGCC AAATGAGACG TTCGAAAAAT CGGTGCAGCT CCTGGATCAA
    ATTCCTTCGT ATGACACCCA CAAGATCGCA GTGCTGTACG TTGGAGAGGG CCAGAGCAAC
    AATGAAATCG CTATTCTGTC CAATGAACAC GGCTCCTATC GCTACACCGA GTTCCTGACA
    GGCTTAGGGA AGCTCATTGA GCTCAAAGAC TGCCAGCCAG ATAAAATCTA CCTCGGTGGC
    CTGGACGTGT GTGGGGAGGA TGGACAGTTT ACCTACTGCT GGCACGATGA CATCATGCAA
    GCTATTTTCC ACATTGCTAC ACTGATGCCC ACAAAGGATT TGGATAAATA TCGCTGTGAC
    AAGAAGAGGC ACCTTGGGAA TGATTTTGTT TCTATTGTTT ACAATGATTC TGGTGAGGAC
    TTCAAACTGG GAACAATAAA GGGTCAGTTC AATTTTGTGC ATGTTATCAT AACCCCACTT
    GACTATGACT GCAACCTGGT CACACTGCAG TGTCGAAAAG ACATGGAGGG CCTTGTAGAC
    ACGAGTGTTG CTAAGATCAT CTCTGACAAG AACCTGCCAT TCGTGGCCCG TCAGATGGCC
    CTGCATGCTA ACATGGCTTC CCAGGTGCAC CACAGTCGAT CCAACCCTAC TGACACCTAC
    CCATCCAAAT GGATCGCTCG GCTGCGGCAC ATCAAACGGC TGAGGCACCG GCTACGTGAA
    GAAACCCAGT ACCAAACTCC TGGCCTTCCC CTGCAGATGC ACCCGTCTGC TCCAACAAAG
    CCCTCTCCCC AGATGGCTCA GGACCCACCG CCGGCCTACG AGACCGGTCA GAGGAAGCGG
    CTGATCTCCT CGGTGGATGA CTTCACTGAG TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149933.1
    CDS58..5556
    Translation

    Target ORF information:

    RefSeq Version XM_015294447.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TTTAAGACTA GCCAAAAATG CAAAGCAAGG CTTGAATAAC
    TCTCCTCCAG TGAAAGAGCT GAAAGAACCC TCTGCAGTTG ATGCCTTCCG ATCCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGT CGGATACAGA CATCCATCAC TAGTTCCAGC
    TTGGGCTCTG CAGATGAAAA TTCAATGGCT CAGGCTGATG ACAACTTAAA AAATCTCCAT
    CTGGAACTCA CTGAGACTTG TCTGGATATG ATGGCCCGAT ACGTCTTTTC CAACTTCACT
    GCGGTGCCTA AGAGGTCTCC TGTGGGTGAG TTTCTGTTGG CTGGAGGAAG GACAAAGACA
    TGGCTGGTTG GCAACAAGTT GGTGACCATC ACTACAAGTG TCGGAACAGG GACAAGGTCC
    TTGCTTGGCC TGGACTCTGG AGATCTCCAA AGCAGCACAG AATCAGGCTC AGACCCGGTT
    TTGCAAGTGA GACAAACTAA AGAAGCTCCA GCAAAGCTCG AATCTCAAGC TGGGCAGCAG
    GTTTGCAGAA GCTCTCGCAA CAGAGTCAGA TCCATGTCTG GTGGTCATGC CTTACGTGTT
    GGTGCCTTAG ACAGCACAGC CTCCCACTTC CCTGGTGGCT CGGCTTCACA AGGGACACAG
    AGTCCTCCTG CTCCTGCACC TCGGTCAGAG AAGACTAATC CTGCTCCACA GACACCTCTG
    CAGAAAGAAA AAGCGAACTT AGCTGCATAT GTCCCACTGC TGACACAGGG CTGGGCAGAA
    ATCCTTGTGC GCAGACCCAC AGGTAACACA AGCTGGCTAA TGAGTCTGGA GAACCCGCTC
    AGTCCCTTTT CTTCAGACAT TAACAATATG CCCCTGCAAG AGCTGTCCAA CGCACTCATG
    GCTGCAGAGC GTTTTAAAGA GCACCGAGAA ACAGCTCTCT ACAAATCCCT GTCTGTCCCT
    TCCTCCAGCT TGGCCACTGG GACAGCCAAG CCTTCACTTC TCCAGCGTTC CAACACAGTG
    GCCTCTTTCT CTTCCATGTA CCAGTCCAGT TGTCAAGGGA AGTTGCACAG GAGCATATCC
    TGGGCAGAGT CTGCGGTCGT GCTGGAGGAA GGGAGCCCCT TGGAAATGGA GCTGAAGGAA
    ACTGAGTCCC CAGCTGAGTC TCCAGAGAGT GAAGATTTTG AGACGTCGTG TTCTGAGCAA
    GTCCTGAGCG AGGAAAGGTT TGGTAAAGCC CAGGAGTTTT ACAGCAGGTC GTCCTCGACC
    TCTAGCCAAG ATGAAAAATC TCTGAAGTCG GACGAGCTGG TGGAGGGCGG TATTCCCATC
    GGGAGAGTGC TGCCAGCAGA GGATGGCCGG GCACTGGAGG AGCTCTCCTT CCAGCCCTCC
    CAGCCGCTCA GCAAATCGAG CTCTTCCCCC GAGCTGCAGA CTTTGCAGGA GATCCTCAAG
    GATGCAAATG GCAGAGAAGT AACAAGGAGG CTGAGCACGG AAGTGAAGTC CAAATCTCAG
    TCTGGGAACC TGGAAGGGGA AGGACTGGGC AGTTGGCTGA ACAAGGGTGA GGATGCCAGA
    GCAGCAGGCT CGGGGGGGTT GGATGGCAGT GGCCCCACCA CCTCGCCACG CTCCCCATCT
    GGGCACCGGC CCCGGGGGTA CACCATCTCT GACTCTGCCC CATCACGGAG AGGGAAGAGG
    ATGGAGAGAG ATGCCTTCAA GAGCAGAACA GCAGCCACCA ATGCTGAGAA AGTACCTGGA
    ATAAACCCAA GCTTTGTGTT TTTACAATTG TACCATTCCC CGTTCTTTGG TGATGAAAAC
    AACAAGCCCC TCCTGTTGCC AAATGAGACG TTCGAAAAAT CGGTGCAGCT CCTGGATCAA
    ATTCCTTCGT ATGACACCCA CAAGATCGCA GTGCTGTACG TTGGAGAGGG CCAGAGCAAC
    AATGAAATCG CTATTCTGTC CAATGAACAC GGCTCCTATC GCTACACCGA GTTCCTGACA
    GGCTTAGGGA AGCTCATTGA GCTCAAAGAC TGCCAGCCAG ATAAAATCTA CCTCGGTGGC
    CTGGACGTGT GTGGGGAGGA TGGACAGTTT ACCTACTGCT GGCACGATGA CATCATGCAA
    GCTATTTTCC ACATTGCTAC ACTGATGCCC ACAAAGGATT TGGATAAATA TCGCTGTGAC
    AAGAAGAGGC ACCTTGGGAA TGATTTTGTT TCTATTGTTT ACAATGATTC TGGTGAGGAC
    TTCAAACTGG GAACAATAAA GGGTCAGTTC AATTTTGTGC ATGTTATCAT AACCCCACTT
    GACTATGACT GCAACCTGGT CACACTGCAG TGTCGAAAAG ACATGGAGGG CCTTGTAGAC
    ACGAGTGTTG CTAAGATCAT CTCTGACAAG AACCTGCCAT TCGTGGCCCG TCAGATGGCC
    CTGCATGCTA ACATGGCTTC CCAGGTGCAC CACAGTCGAT CCAACCCTAC TGACACCTAC
    CCATCCAAAT GGATCGCTCG GCTGCGGCAC ATCAAACGGC TGAGGCACCG GCTACGTGAA
    GAAACCCAGT ACCAAACTCC TGGCCTTCCC CTGCAGATGC ACCCGTCTGC TCCAACAAAG
    CCCTCTCCCC AGATGGCTCA GGACCCACCG CCGGCCTACG AGACCGGTCA GAGGAAGCGG
    CTGATCTCCT CGGTGGATGA CTTCACTGAG TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42374
    Clone ID Related Accession (Same CDS sequence) XM_015294448.1 , XM_015294448.2
    Accession Version XM_015294448.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tuberin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGTTA TTAAGGACTA TCCTTCCAAT
    GAAGACTTGC ATGAACGGCT TGAAGTTTTT AAGGCTCTAA CAGAGAATGG ACGATACATC
    ACTTACTTAG AAGAAGAGTT AGCTGACTTC GTGCTACAGT GGATGGATGT TGGTTTGTCT
    TCAGAGTTCC TTTTGGTGTT AGTGAACCTG GTGAAATTCA ATAGCTGCTA TTTGGAAGAC
    TACGTAGCTG ACATGGTCCA CAAGATATGC ATCTTGTGTA TTCAGACTTC ATCATCTGTG
    GACATAGAGA TTTCTTTGCA AGTTTTAGAT GCTGTTGTTT GCTATAACTG TCTGCCGTCA
    GAGAACCTGC CAGTATTTAT CATCACTTTG TGCCGTACCA TCAATGTGAA GGAGCTCTGT
    GAGCCATGCT GGAAGCTTAT GCGCAACCTT TTGGGAACTC ATTTGGGACA CAGTGCAATT
    TACAACATGT GCAGGATCAT GGAAGACAGA GCTTACATGG CAGATGCAGC CCTGCTGAGA
    GGAGCGGTGT TCTTTGTTGG GATGGCTCTC TGGGGTGCTC ATCGCCTCAA TTCACTCAAG
    AATTCCCCAA CTTCAGTGCT GCCTTCGTTC CTCAAGGCCA TGACGTGTCC CAATGCAGTT
    GTATCTTATG AGATAGTTCT GTCCATCACC CGTCTAATAA AGAAGTATGG GAAAGAACTG
    CAAGCTGTAA CCTGGGATAT CCTCTTGGAC ATCATGGAGC GATTACTGCA ACAGTTGCAG
    AGTTTAGAGA GCCAAGAACT TAAGTCCATT GTACATGATC TTTTGACTAC AGTAGAAGAA
    CTCTGTGACC AGAATGAATT TCATGGTTCT GAGGAGAGAT TTTTTGAGCT GGTGGAAAGA
    TGCGCTGATC AGAGACCGGA ATCTTCCGTA TTAAACTTGA TAGCATACAG AGCTCAATCC
    ATTCATCCTG CCAAAGATGG CTGGATTCAC AACCTGCAGA TGTTAATGGA GAGATTCTTC
    AGGAACGAAA GTCGTAGTGC TGTTCGTATT AAAGTTCTGG ATGTCTTGTC CTTTGTACTG
    AGTATTAACA GACAGTTCTA TGAGGAAGAG CTGATAAACT TGGTTGTGAT CTCTCAGCTG
    GCTCACATTC CAGAGGATAA AGACCATCAA GTTCGAAAAC TTGCCACTCA GCTACTTGTA
    GACCTTGCTG AAGGCTGCAA CACACATCAC TTCAACAGCT TGCTTGATAT CATAGAAAAG
    GTGGCTGCGC ATTCTCTCTC ATCTCCATCT GAACTGGAAG AGAGGGATTT GCTATCATAT
    TCAGCTTCTT TGGAGGACGT GAAGACAGCA GTTCTTGGAC TTCTGATAAT TCTTCAGACA
    AAACTCTACA GCTTACCTTC CAGCCATGCC ATGCGGGTGT ATGAGATGCT GATTCACCAC
    ATCCAGCGCC ACTATATGTA TGCGTACAGC CTTGCTGTTG CTAGCAGCAT CAGACTGCAG
    GTGTTTGATT TCCTCCTGAT GCTTCGAGCT GACTCCCTCC ACCGTCTTGG CCTTTCTAAC
    AAGGATGGGG CAGTGAGATT CAGCCCTTAC TGTCTGTGTG ATTTTGTAGA AGCAGAGAAG
    AGAGCTTCTG AGAAAAAGCC TACTGGTACG CTCTCTCCAC CTTCAGGAAG TCCCAGTGTG
    CCTTCCCAGA ACAGCACCAT TCGAATAGGG CATTTGCCAT ACTCAATGGT CTTTGGTGTC
    CTTCTGCAGT GTTTGAAGCA AGAGACAGAC TGGAAGGTGT TGAAGTTGGT CCTCAACAAA
    CTACCAGAAT CACTCCGCTA TAAAGTGCTG TTTTTAACCT CTCCTTGTAA CATAGACCTC
    CTGGCATCTG CACTGAGCTA CATGCTCACA GACAAGAAGA CAACCGACAG GCTCCATGGT
    ACCCCAGAAG GCTTTTCCCG CACCGATTTG CACCTGGCTG TAGTTCCAGT ACTGACAGCG
    TTGATATCTT ATCATAATTA TCTGGACAAA GCTAAACAGC GTGAAATAGT GTACTGCCTG
    GAGCATGGCC TTATTTATCG CTGTGCAAAC CAGTGCGTTG TGGCACTCTC TGTCTGCAGC
    GTTGAGATGC CCGACATCAT CATAAAGGCA CTTCCAGTCT TAATAGTCAA ATTAACCCAC
    ATATCTGCTA CAGCCAACAT GGCAATCCCT CTCTTAGAAT TTCTTTCAAC TCTAGCAAGG
    CTGCCTCACC TCTACAGGAA CTTTGCTGCA GAGCAGTATG CCAGTGTGTT TGCCATCTCC
    CTGCCATACA CAAACCCTTC CAAGTTTAAC CAGTACATTG TTTGCCTGGC CCACCACGTG
    ATAGCTATGT GGTTCATTCG GTGTCGTCTT CCCTTCCGAA AGGACTTTGT TCCCTATATT
    ACAAAGGGTT TGCGCTCCAA TGTGCTGCTC TCCTTTGATG ACACACCTGA GAAGGACAGT
    TTCAGGGCTC GTAGTACAAG CCTCAATGAG AGGCCAAAAA GTAGTTTAAG ACTAGCCAAA
    AATGCAAAGC AAGGCTTGAA TAACTCTCCT CCAGTGAAAG AGCTGAAAGA ACCCTCTGCA
    GTTGATGCCT TCCGATCCCG CAGCATCAGT GTGTCTGAAC ATGTGGTCCG CAGTCGGATA
    CAGACATCCA TCACTAGTTC CAGCTTGGGC TCTGCAGATG AAAATTCAAT GGCTCAGGCT
    GATGACAACT TAAAAAATCT CCATCTGGAA CTCACTGAGA CTTGTCTGGA TATGATGGCC
    CGATACGTCT TTTCCAACTT CACTGCGGTG CCTAAGAGGT CTCCTGTGGG TGAGTTTCTG
    TTGGCTGGAG GAAGGACAAA GACATGGCTG GTTGGCAACA AGTTGGTGAC CATCACTACA
    AGTGTCGGAA CAGGGACAAG GTCCTTGCTT GGCCTGGACT CTGGAGATCT CCAAAGCAGC
    ACAGAATCAG GCTCAGACCC GGTTTTGCAA GTGAGACAAA CTAAAGAAGC TCCAGCAAAG
    CTCGAATCTC AAGCTGGGCA GCAGGTTTGC AGAAGCTCTC GCAACAGAGT CAGATCCATG
    TCTGGTGGTC ATGCCTTACG TGTTGGTGCC TTAGACAGCA CAGCCTCCCA CTTCCCTGGT
    GGCTCGGCTT CACAAGGGAC ACAGAGTCCT CCTGCTCCTG CACCTCGGTC AGAGAAGACT
    AATCCTGCTC CACAGACACC TCTGCAGAAA GAAAAAGCGA ACTTAGCTGC ATATGTCCCA
    CTGCTGACAC AGGGCTGGGC AGAAATCCTT GTGCGCAGAC CCACAGGTAA CACAAGCTGG
    CTAATGAGTC TGGAGAACCC GCTCAGTCCC TTTTCTTCAG ACATTAACAA TATGCCCCTG
    CAAGAGCTGT CCAACGCACT CATGGCTGCA GAGCGTTTTA AAGAGCACCG AGAAACAGCT
    CTCTACAAAT CCCTGTCTGT CCCTTCCTCC AGCTTGGCCA CTGGGACAGC CAAGCCTTCA
    CTTCTCCAGC GTTCCAACAC AGTGGCCTCT TTCTCTTCCA TGTACCAGTC CAGTTGTCAA
    GGGAAGTTGC ACAGGAGCAT ATCCTGGGCA GAGTCTGCGG TCGTGCTGGA GGAAGGGAGC
    CCCTTGGAAA TGGAGCTGAA GGAAACTGAG TCCCCAGCTG AGTCTCCAGA GAGTGAAGAT
    TTTGAGACGT CGTGTTCTGA GCAAGTCCTG AGCGAGGAAA GGTTTGGTAA AGCCCAGGAG
    TTTTACAGCA GGTCGTCCTC GACCTCTAGC CAAGATGAAA AATCTCTGAA GTCGGACGAG
    CTGGTGGAGG GCGGTATTCC CATCGGGAGA GTGCTGCCAG CAGAGGATGG CCGGGCACTG
    GAGGAGCTCT CCTTCCAGCC CTCCCAGCCG CTCAGCAAAT CGAGCTCTTC CCCCGAGCTG
    CAGACTTTGC AGGAGATCCT CAAGGATGCA AATGGCAGAG AAGTAACAAG GAGGCTGAGC
    ACGGAAGTGA AGTCCAAATC TCAGTCTGGG AACCTGGAAG GGGAAGGACT GGGCAGTTGG
    CTGAACAAGG GTGAGGATGC CAGAGCAGCA GGCTCGGGGG GGTTGGATGG CAGTGGCCCC
    ACCACCTCGC CACGCTCCCC ATCTGGGCAC CGGCCCCGGG GGTACACCAT CTCTGACTCT
    GCCCCATCAC GGAGAGGGAA GAGGATGGAG AGAGATGCCT TCAAGAGCAG AACAGCAGCC
    ACCAATGCTG AGAAAGTACC TGGAATAAAC CCAAGCTTTG TGTTTTTACA ATTGTACCAT
    TCCCCGTTCT TTGGTGATGA AAACAACAAG CCCCTCCTGT TGCCAAATGA GACGTTCGAA
    AAATCGGTGC AGCTCCTGGA TCAAATTCCT TCGTATGACA CCCACAAGAT CGCAGTGCTG
    TACGTTGGAG AGGGCCAGAG CAACAATGAA ATCGCTATTC TGTCCAATGA ACACGGCTCC
    TATCGCTACA CCGAGTTCCT GACAGGCTTA GGGAAGCTCA TTGAGCTCAA AGACTGCCAG
    CCAGATAAAA TCTACCTCGG TGGCCTGGAC GTGTGTGGGG AGGATGGACA GTTTACCTAC
    TGCTGGCACG ATGACATCAT GCAAGCTATT TTCCACATTG CTACACTGAT GCCCACAAAG
    GATTTGGATA AATATCGCTG TGACAAGAAG AGGCACCTTG GGAATGATTT TGTTTCTATT
    GTTTACAATG ATTCTGGTGA GGACTTCAAA CTGGGAACAA TAAAGGGTCA GTTCAATTTT
    GTGCATGTTA TCATAACCCC ACTTGACTAT GACTGCAACC TGGTCACACT GCAGTGTCGA
    AAAGACATGG AGGGCCTTGT AGACACGAGT GTTGCTAAGA TCATCTCTGA CAAGAACCTG
    CCATTCGTGG CCCGTCAGAT GGCCCTGCAT GCTAACATGG CTTCCCAGGT GCACCACAGT
    CGATCCAACC CTACTGACAC CTACCCATCC AAATGGATCG CTCGGCTGCG GCACATCAAA
    CGGCTGAGGC ACCGGCTACG TGAAGAAACC CAGTACCAAA CTCCTGGCCT TCCCCTGCAG
    ATGCACCCGT CTGCTCCAAC AAAGCCCTCT CCCCAGATGG CTCAGGACCC ACCGCCGGCC
    TACGAGACCG GTCAGAGGAA GCGGCTGATC TCCTCGGTGG ATGACTTCAC TGAGTTTGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149934.1
    CDS58..5520
    Translation

    Target ORF information:

    RefSeq Version XM_015294448.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGTTA TTAAGGACTA TCCTTCCAAT
    GAAGACTTGC ATGAACGGCT TGAAGTTTTT AAGGCTCTAA CAGAGAATGG ACGATACATC
    ACTTACTTAG AAGAAGAGTT AGCTGACTTC GTGCTACAGT GGATGGATGT TGGTTTGTCT
    TCAGAGTTCC TTTTGGTGTT AGTGAACCTG GTGAAATTCA ATAGCTGCTA TTTGGAAGAC
    TACGTAGCTG ACATGGTCCA CAAGATATGC ATCTTGTGTA TTCAGACTTC ATCATCTGTG
    GACATAGAGA TTTCTTTGCA AGTTTTAGAT GCTGTTGTTT GCTATAACTG TCTGCCGTCA
    GAGAACCTGC CAGTATTTAT CATCACTTTG TGCCGTACCA TCAATGTGAA GGAGCTCTGT
    GAGCCATGCT GGAAGCTTAT GCGCAACCTT TTGGGAACTC ATTTGGGACA CAGTGCAATT
    TACAACATGT GCAGGATCAT GGAAGACAGA GCTTACATGG CAGATGCAGC CCTGCTGAGA
    GGAGCGGTGT TCTTTGTTGG GATGGCTCTC TGGGGTGCTC ATCGCCTCAA TTCACTCAAG
    AATTCCCCAA CTTCAGTGCT GCCTTCGTTC CTCAAGGCCA TGACGTGTCC CAATGCAGTT
    GTATCTTATG AGATAGTTCT GTCCATCACC CGTCTAATAA AGAAGTATGG GAAAGAACTG
    CAAGCTGTAA CCTGGGATAT CCTCTTGGAC ATCATGGAGC GATTACTGCA ACAGTTGCAG
    AGTTTAGAGA GCCAAGAACT TAAGTCCATT GTACATGATC TTTTGACTAC AGTAGAAGAA
    CTCTGTGACC AGAATGAATT TCATGGTTCT GAGGAGAGAT TTTTTGAGCT GGTGGAAAGA
    TGCGCTGATC AGAGACCGGA ATCTTCCGTA TTAAACTTGA TAGCATACAG AGCTCAATCC
    ATTCATCCTG CCAAAGATGG CTGGATTCAC AACCTGCAGA TGTTAATGGA GAGATTCTTC
    AGGAACGAAA GTCGTAGTGC TGTTCGTATT AAAGTTCTGG ATGTCTTGTC CTTTGTACTG
    AGTATTAACA GACAGTTCTA TGAGGAAGAG CTGATAAACT TGGTTGTGAT CTCTCAGCTG
    GCTCACATTC CAGAGGATAA AGACCATCAA GTTCGAAAAC TTGCCACTCA GCTACTTGTA
    GACCTTGCTG AAGGCTGCAA CACACATCAC TTCAACAGCT TGCTTGATAT CATAGAAAAG
    GTGGCTGCGC ATTCTCTCTC ATCTCCATCT GAACTGGAAG AGAGGGATTT GCTATCATAT
    TCAGCTTCTT TGGAGGACGT GAAGACAGCA GTTCTTGGAC TTCTGATAAT TCTTCAGACA
    AAACTCTACA GCTTACCTTC CAGCCATGCC ATGCGGGTGT ATGAGATGCT GATTCACCAC
    ATCCAGCGCC ACTATATGTA TGCGTACAGC CTTGCTGTTG CTAGCAGCAT CAGACTGCAG
    GTGTTTGATT TCCTCCTGAT GCTTCGAGCT GACTCCCTCC ACCGTCTTGG CCTTTCTAAC
    AAGGATGGGG CAGTGAGATT CAGCCCTTAC TGTCTGTGTG ATTTTGTAGA AGCAGAGAAG
    AGAGCTTCTG AGAAAAAGCC TACTGGTACG CTCTCTCCAC CTTCAGGAAG TCCCAGTGTG
    CCTTCCCAGA ACAGCACCAT TCGAATAGGG CATTTGCCAT ACTCAATGGT CTTTGGTGTC
    CTTCTGCAGT GTTTGAAGCA AGAGACAGAC TGGAAGGTGT TGAAGTTGGT CCTCAACAAA
    CTACCAGAAT CACTCCGCTA TAAAGTGCTG TTTTTAACCT CTCCTTGTAA CATAGACCTC
    CTGGCATCTG CACTGAGCTA CATGCTCACA GACAAGAAGA CAACCGACAG GCTCCATGGT
    ACCCCAGAAG GCTTTTCCCG CACCGATTTG CACCTGGCTG TAGTTCCAGT ACTGACAGCG
    TTGATATCTT ATCATAATTA TCTGGACAAA GCTAAACAGC GTGAAATAGT GTACTGCCTG
    GAGCATGGCC TTATTTATCG CTGTGCAAAC CAGTGCGTTG TGGCACTCTC TGTCTGCAGC
    GTTGAGATGC CCGACATCAT CATAAAGGCA CTTCCAGTCT TAATAGTCAA ATTAACCCAC
    ATATCTGCTA CAGCCAACAT GGCAATCCCT CTCTTAGAAT TTCTTTCAAC TCTAGCAAGG
    CTGCCTCACC TCTACAGGAA CTTTGCTGCA GAGCAGTATG CCAGTGTGTT TGCCATCTCC
    CTGCCATACA CAAACCCTTC CAAGTTTAAC CAGTACATTG TTTGCCTGGC CCACCACGTG
    ATAGCTATGT GGTTCATTCG GTGTCGTCTT CCCTTCCGAA AGGACTTTGT TCCCTATATT
    ACAAAGGGTT TGCGCTCCAA TGTGCTGCTC TCCTTTGATG ACACACCTGA GAAGGACAGT
    TTCAGGGCTC GTAGTACAAG CCTCAATGAG AGGCCAAAAA GTAGTTTAAG ACTAGCCAAA
    AATGCAAAGC AAGGCTTGAA TAACTCTCCT CCAGTGAAAG AGCTGAAAGA ACCCTCTGCA
    GTTGATGCCT TCCGATCCCG CAGCATCAGT GTGTCTGAAC ATGTGGTCCG CAGTCGGATA
    CAGACATCCA TCACTAGTTC CAGCTTGGGC TCTGCAGATG AAAATTCAAT GGCTCAGGCT
    GATGACAACT TAAAAAATCT CCATCTGGAA CTCACTGAGA CTTGTCTGGA TATGATGGCC
    CGATACGTCT TTTCCAACTT CACTGCGGTG CCTAAGAGGT CTCCTGTGGG TGAGTTTCTG
    TTGGCTGGAG GAAGGACAAA GACATGGCTG GTTGGCAACA AGTTGGTGAC CATCACTACA
    AGTGTCGGAA CAGGGACAAG GTCCTTGCTT GGCCTGGACT CTGGAGATCT CCAAAGCAGC
    ACAGAATCAG GCTCAGACCC GGTTTTGCAA GTGAGACAAA CTAAAGAAGC TCCAGCAAAG
    CTCGAATCTC AAGCTGGGCA GCAGGTTTGC AGAAGCTCTC GCAACAGAGT CAGATCCATG
    TCTGGTGGTC ATGCCTTACG TGTTGGTGCC TTAGACAGCA CAGCCTCCCA CTTCCCTGGT
    GGCTCGGCTT CACAAGGGAC ACAGAGTCCT CCTGCTCCTG CACCTCGGTC AGAGAAGACT
    AATCCTGCTC CACAGACACC TCTGCAGAAA GAAAAAGCGA ACTTAGCTGC ATATGTCCCA
    CTGCTGACAC AGGGCTGGGC AGAAATCCTT GTGCGCAGAC CCACAGGTAA CACAAGCTGG
    CTAATGAGTC TGGAGAACCC GCTCAGTCCC TTTTCTTCAG ACATTAACAA TATGCCCCTG
    CAAGAGCTGT CCAACGCACT CATGGCTGCA GAGCGTTTTA AAGAGCACCG AGAAACAGCT
    CTCTACAAAT CCCTGTCTGT CCCTTCCTCC AGCTTGGCCA CTGGGACAGC CAAGCCTTCA
    CTTCTCCAGC GTTCCAACAC AGTGGCCTCT TTCTCTTCCA TGTACCAGTC CAGTTGTCAA
    GGGAAGTTGC ACAGGAGCAT ATCCTGGGCA GAGTCTGCGG TCGTGCTGGA GGAAGGGAGC
    CCCTTGGAAA TGGAGCTGAA GGAAACTGAG TCCCCAGCTG AGTCTCCAGA GAGTGAAGAT
    TTTGAGACGT CGTGTTCTGA GCAAGTCCTG AGCGAGGAAA GGTTTGGTAA AGCCCAGGAG
    TTTTACAGCA GGTCGTCCTC GACCTCTAGC CAAGATGAAA AATCTCTGAA GTCGGACGAG
    CTGGTGGAGG GCGGTATTCC CATCGGGAGA GTGCTGCCAG CAGAGGATGG CCGGGCACTG
    GAGGAGCTCT CCTTCCAGCC CTCCCAGCCG CTCAGCAAAT CGAGCTCTTC CCCCGAGCTG
    CAGACTTTGC AGGAGATCCT CAAGGATGCA AATGGCAGAG AAGTAACAAG GAGGCTGAGC
    ACGGAAGTGA AGTCCAAATC TCAGTCTGGG AACCTGGAAG GGGAAGGACT GGGCAGTTGG
    CTGAACAAGG GTGAGGATGC CAGAGCAGCA GGCTCGGGGG GGTTGGATGG CAGTGGCCCC
    ACCACCTCGC CACGCTCCCC ATCTGGGCAC CGGCCCCGGG GGTACACCAT CTCTGACTCT
    GCCCCATCAC GGAGAGGGAA GAGGATGGAG AGAGATGCCT TCAAGAGCAG AACAGCAGCC
    ACCAATGCTG AGAAAGTACC TGGAATAAAC CCAAGCTTTG TGTTTTTACA ATTGTACCAT
    TCCCCGTTCT TTGGTGATGA AAACAACAAG CCCCTCCTGT TGCCAAATGA GACGTTCGAA
    AAATCGGTGC AGCTCCTGGA TCAAATTCCT TCGTATGACA CCCACAAGAT CGCAGTGCTG
    TACGTTGGAG AGGGCCAGAG CAACAATGAA ATCGCTATTC TGTCCAATGA ACACGGCTCC
    TATCGCTACA CCGAGTTCCT GACAGGCTTA GGGAAGCTCA TTGAGCTCAA AGACTGCCAG
    CCAGATAAAA TCTACCTCGG TGGCCTGGAC GTGTGTGGGG AGGATGGACA GTTTACCTAC
    TGCTGGCACG ATGACATCAT GCAAGCTATT TTCCACATTG CTACACTGAT GCCCACAAAG
    GATTTGGATA AATATCGCTG TGACAAGAAG AGGCACCTTG GGAATGATTT TGTTTCTATT
    GTTTACAATG ATTCTGGTGA GGACTTCAAA CTGGGAACAA TAAAGGGTCA GTTCAATTTT
    GTGCATGTTA TCATAACCCC ACTTGACTAT GACTGCAACC TGGTCACACT GCAGTGTCGA
    AAAGACATGG AGGGCCTTGT AGACACGAGT GTTGCTAAGA TCATCTCTGA CAAGAACCTG
    CCATTCGTGG CCCGTCAGAT GGCCCTGCAT GCTAACATGG CTTCCCAGGT GCACCACAGT
    CGATCCAACC CTACTGACAC CTACCCATCC AAATGGATCG CTCGGCTGCG GCACATCAAA
    CGGCTGAGGC ACCGGCTACG TGAAGAAACC CAGTACCAAA CTCCTGGCCT TCCCCTGCAG
    ATGCACCCGT CTGCTCCAAC AAAGCCCTCT CCCCAGATGG CTCAGGACCC ACCGCCGGCC
    TACGAGACCG GTCAGAGGAA GCGGCTGATC TCCTCGGTGG ATGACTTCAC TGAGTTTGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42374
    Clone ID Related Accession (Same CDS sequence) XM_015294448.1 , XM_015294448.2
    Accession Version XM_015294448.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tuberin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGTTA TTAAGGACTA TCCTTCCAAT
    GAAGACTTGC ATGAACGGCT TGAAGTTTTT AAGGCTCTAA CAGAGAATGG ACGATACATC
    ACTTACTTAG AAGAAGAGTT AGCTGACTTC GTGCTACAGT GGATGGATGT TGGTTTGTCT
    TCAGAGTTCC TTTTGGTGTT AGTGAACCTG GTGAAATTCA ATAGCTGCTA TTTGGAAGAC
    TACGTAGCTG ACATGGTCCA CAAGATATGC ATCTTGTGTA TTCAGACTTC ATCATCTGTG
    GACATAGAGA TTTCTTTGCA AGTTTTAGAT GCTGTTGTTT GCTATAACTG TCTGCCGTCA
    GAGAACCTGC CAGTATTTAT CATCACTTTG TGCCGTACCA TCAATGTGAA GGAGCTCTGT
    GAGCCATGCT GGAAGCTTAT GCGCAACCTT TTGGGAACTC ATTTGGGACA CAGTGCAATT
    TACAACATGT GCAGGATCAT GGAAGACAGA GCTTACATGG CAGATGCAGC CCTGCTGAGA
    GGAGCGGTGT TCTTTGTTGG GATGGCTCTC TGGGGTGCTC ATCGCCTCAA TTCACTCAAG
    AATTCCCCAA CTTCAGTGCT GCCTTCGTTC CTCAAGGCCA TGACGTGTCC CAATGCAGTT
    GTATCTTATG AGATAGTTCT GTCCATCACC CGTCTAATAA AGAAGTATGG GAAAGAACTG
    CAAGCTGTAA CCTGGGATAT CCTCTTGGAC ATCATGGAGC GATTACTGCA ACAGTTGCAG
    AGTTTAGAGA GCCAAGAACT TAAGTCCATT GTACATGATC TTTTGACTAC AGTAGAAGAA
    CTCTGTGACC AGAATGAATT TCATGGTTCT GAGGAGAGAT TTTTTGAGCT GGTGGAAAGA
    TGCGCTGATC AGAGACCGGA ATCTTCCGTA TTAAACTTGA TAGCATACAG AGCTCAATCC
    ATTCATCCTG CCAAAGATGG CTGGATTCAC AACCTGCAGA TGTTAATGGA GAGATTCTTC
    AGGAACGAAA GTCGTAGTGC TGTTCGTATT AAAGTTCTGG ATGTCTTGTC CTTTGTACTG
    AGTATTAACA GACAGTTCTA TGAGGAAGAG CTGATAAACT TGGTTGTGAT CTCTCAGCTG
    GCTCACATTC CAGAGGATAA AGACCATCAA GTTCGAAAAC TTGCCACTCA GCTACTTGTA
    GACCTTGCTG AAGGCTGCAA CACACATCAC TTCAACAGCT TGCTTGATAT CATAGAAAAG
    GTGGCTGCGC ATTCTCTCTC ATCTCCATCT GAACTGGAAG AGAGGGATTT GCTATCATAT
    TCAGCTTCTT TGGAGGACGT GAAGACAGCA GTTCTTGGAC TTCTGATAAT TCTTCAGACA
    AAACTCTACA GCTTACCTTC CAGCCATGCC ATGCGGGTGT ATGAGATGCT GATTCACCAC
    ATCCAGCGCC ACTATATGTA TGCGTACAGC CTTGCTGTTG CTAGCAGCAT CAGACTGCAG
    GTGTTTGATT TCCTCCTGAT GCTTCGAGCT GACTCCCTCC ACCGTCTTGG CCTTTCTAAC
    AAGGATGGGG CAGTGAGATT CAGCCCTTAC TGTCTGTGTG ATTTTGTAGA AGCAGAGAAG
    AGAGCTTCTG AGAAAAAGCC TACTGGTACG CTCTCTCCAC CTTCAGGAAG TCCCAGTGTG
    CCTTCCCAGA ACAGCACCAT TCGAATAGGG CATTTGCCAT ACTCAATGGT CTTTGGTGTC
    CTTCTGCAGT GTTTGAAGCA AGAGACAGAC TGGAAGGTGT TGAAGTTGGT CCTCAACAAA
    CTACCAGAAT CACTCCGCTA TAAAGTGCTG TTTTTAACCT CTCCTTGTAA CATAGACCTC
    CTGGCATCTG CACTGAGCTA CATGCTCACA GACAAGAAGA CAACCGACAG GCTCCATGGT
    ACCCCAGAAG GCTTTTCCCG CACCGATTTG CACCTGGCTG TAGTTCCAGT ACTGACAGCG
    TTGATATCTT ATCATAATTA TCTGGACAAA GCTAAACAGC GTGAAATAGT GTACTGCCTG
    GAGCATGGCC TTATTTATCG CTGTGCAAAC CAGTGCGTTG TGGCACTCTC TGTCTGCAGC
    GTTGAGATGC CCGACATCAT CATAAAGGCA CTTCCAGTCT TAATAGTCAA ATTAACCCAC
    ATATCTGCTA CAGCCAACAT GGCAATCCCT CTCTTAGAAT TTCTTTCAAC TCTAGCAAGG
    CTGCCTCACC TCTACAGGAA CTTTGCTGCA GAGCAGTATG CCAGTGTGTT TGCCATCTCC
    CTGCCATACA CAAACCCTTC CAAGTTTAAC CAGTACATTG TTTGCCTGGC CCACCACGTG
    ATAGCTATGT GGTTCATTCG GTGTCGTCTT CCCTTCCGAA AGGACTTTGT TCCCTATATT
    ACAAAGGGTT TGCGCTCCAA TGTGCTGCTC TCCTTTGATG ACACACCTGA GAAGGACAGT
    TTCAGGGCTC GTAGTACAAG CCTCAATGAG AGGCCAAAAA GTAGTTTAAG ACTAGCCAAA
    AATGCAAAGC AAGGCTTGAA TAACTCTCCT CCAGTGAAAG AGCTGAAAGA ACCCTCTGCA
    GTTGATGCCT TCCGATCCCG CAGCATCAGT GTGTCTGAAC ATGTGGTCCG CAGTCGGATA
    CAGACATCCA TCACTAGTTC CAGCTTGGGC TCTGCAGATG AAAATTCAAT GGCTCAGGCT
    GATGACAACT TAAAAAATCT CCATCTGGAA CTCACTGAGA CTTGTCTGGA TATGATGGCC
    CGATACGTCT TTTCCAACTT CACTGCGGTG CCTAAGAGGT CTCCTGTGGG TGAGTTTCTG
    TTGGCTGGAG GAAGGACAAA GACATGGCTG GTTGGCAACA AGTTGGTGAC CATCACTACA
    AGTGTCGGAA CAGGGACAAG GTCCTTGCTT GGCCTGGACT CTGGAGATCT CCAAAGCAGC
    ACAGAATCAG GCTCAGACCC GGTTTTGCAA GTGAGACAAA CTAAAGAAGC TCCAGCAAAG
    CTCGAATCTC AAGCTGGGCA GCAGGTTTGC AGAAGCTCTC GCAACAGAGT CAGATCCATG
    TCTGGTGGTC ATGCCTTACG TGTTGGTGCC TTAGACAGCA CAGCCTCCCA CTTCCCTGGT
    GGCTCGGCTT CACAAGGGAC ACAGAGTCCT CCTGCTCCTG CACCTCGGTC AGAGAAGACT
    AATCCTGCTC CACAGACACC TCTGCAGAAA GAAAAAGCGA ACTTAGCTGC ATATGTCCCA
    CTGCTGACAC AGGGCTGGGC AGAAATCCTT GTGCGCAGAC CCACAGGTAA CACAAGCTGG
    CTAATGAGTC TGGAGAACCC GCTCAGTCCC TTTTCTTCAG ACATTAACAA TATGCCCCTG
    CAAGAGCTGT CCAACGCACT CATGGCTGCA GAGCGTTTTA AAGAGCACCG AGAAACAGCT
    CTCTACAAAT CCCTGTCTGT CCCTTCCTCC AGCTTGGCCA CTGGGACAGC CAAGCCTTCA
    CTTCTCCAGC GTTCCAACAC AGTGGCCTCT TTCTCTTCCA TGTACCAGTC CAGTTGTCAA
    GGGAAGTTGC ACAGGAGCAT ATCCTGGGCA GAGTCTGCGG TCGTGCTGGA GGAAGGGAGC
    CCCTTGGAAA TGGAGCTGAA GGAAACTGAG TCCCCAGCTG AGTCTCCAGA GAGTGAAGAT
    TTTGAGACGT CGTGTTCTGA GCAAGTCCTG AGCGAGGAAA GGTTTGGTAA AGCCCAGGAG
    TTTTACAGCA GGTCGTCCTC GACCTCTAGC CAAGATGAAA AATCTCTGAA GTCGGACGAG
    CTGGTGGAGG GCGGTATTCC CATCGGGAGA GTGCTGCCAG CAGAGGATGG CCGGGCACTG
    GAGGAGCTCT CCTTCCAGCC CTCCCAGCCG CTCAGCAAAT CGAGCTCTTC CCCCGAGCTG
    CAGACTTTGC AGGAGATCCT CAAGGATGCA AATGGCAGAG AAGTAACAAG GAGGCTGAGC
    ACGGAAGTGA AGTCCAAATC TCAGTCTGGG AACCTGGAAG GGGAAGGACT GGGCAGTTGG
    CTGAACAAGG GTGAGGATGC CAGAGCAGCA GGCTCGGGGG GGTTGGATGG CAGTGGCCCC
    ACCACCTCGC CACGCTCCCC ATCTGGGCAC CGGCCCCGGG GGTACACCAT CTCTGACTCT
    GCCCCATCAC GGAGAGGGAA GAGGATGGAG AGAGATGCCT TCAAGAGCAG AACAGCAGCC
    ACCAATGCTG AGAAAGTACC TGGAATAAAC CCAAGCTTTG TGTTTTTACA ATTGTACCAT
    TCCCCGTTCT TTGGTGATGA AAACAACAAG CCCCTCCTGT TGCCAAATGA GACGTTCGAA
    AAATCGGTGC AGCTCCTGGA TCAAATTCCT TCGTATGACA CCCACAAGAT CGCAGTGCTG
    TACGTTGGAG AGGGCCAGAG CAACAATGAA ATCGCTATTC TGTCCAATGA ACACGGCTCC
    TATCGCTACA CCGAGTTCCT GACAGGCTTA GGGAAGCTCA TTGAGCTCAA AGACTGCCAG
    CCAGATAAAA TCTACCTCGG TGGCCTGGAC GTGTGTGGGG AGGATGGACA GTTTACCTAC
    TGCTGGCACG ATGACATCAT GCAAGCTATT TTCCACATTG CTACACTGAT GCCCACAAAG
    GATTTGGATA AATATCGCTG TGACAAGAAG AGGCACCTTG GGAATGATTT TGTTTCTATT
    GTTTACAATG ATTCTGGTGA GGACTTCAAA CTGGGAACAA TAAAGGGTCA GTTCAATTTT
    GTGCATGTTA TCATAACCCC ACTTGACTAT GACTGCAACC TGGTCACACT GCAGTGTCGA
    AAAGACATGG AGGGCCTTGT AGACACGAGT GTTGCTAAGA TCATCTCTGA CAAGAACCTG
    CCATTCGTGG CCCGTCAGAT GGCCCTGCAT GCTAACATGG CTTCCCAGGT GCACCACAGT
    CGATCCAACC CTACTGACAC CTACCCATCC AAATGGATCG CTCGGCTGCG GCACATCAAA
    CGGCTGAGGC ACCGGCTACG TGAAGAAACC CAGTACCAAA CTCCTGGCCT TCCCCTGCAG
    ATGCACCCGT CTGCTCCAAC AAAGCCCTCT CCCCAGATGG CTCAGGACCC ACCGCCGGCC
    TACGAGACCG GTCAGAGGAA GCGGCTGATC TCCTCGGTGG ATGACTTCAC TGAGTTTGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149934.1
    CDS59..5521
    Translation

    Target ORF information:

    RefSeq Version XM_015294448.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294448.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGTTA TTAAGGACTA TCCTTCCAAT
    GAAGACTTGC ATGAACGGCT TGAAGTTTTT AAGGCTCTAA CAGAGAATGG ACGATACATC
    ACTTACTTAG AAGAAGAGTT AGCTGACTTC GTGCTACAGT GGATGGATGT TGGTTTGTCT
    TCAGAGTTCC TTTTGGTGTT AGTGAACCTG GTGAAATTCA ATAGCTGCTA TTTGGAAGAC
    TACGTAGCTG ACATGGTCCA CAAGATATGC ATCTTGTGTA TTCAGACTTC ATCATCTGTG
    GACATAGAGA TTTCTTTGCA AGTTTTAGAT GCTGTTGTTT GCTATAACTG TCTGCCGTCA
    GAGAACCTGC CAGTATTTAT CATCACTTTG TGCCGTACCA TCAATGTGAA GGAGCTCTGT
    GAGCCATGCT GGAAGCTTAT GCGCAACCTT TTGGGAACTC ATTTGGGACA CAGTGCAATT
    TACAACATGT GCAGGATCAT GGAAGACAGA GCTTACATGG CAGATGCAGC CCTGCTGAGA
    GGAGCGGTGT TCTTTGTTGG GATGGCTCTC TGGGGTGCTC ATCGCCTCAA TTCACTCAAG
    AATTCCCCAA CTTCAGTGCT GCCTTCGTTC CTCAAGGCCA TGACGTGTCC CAATGCAGTT
    GTATCTTATG AGATAGTTCT GTCCATCACC CGTCTAATAA AGAAGTATGG GAAAGAACTG
    CAAGCTGTAA CCTGGGATAT CCTCTTGGAC ATCATGGAGC GATTACTGCA ACAGTTGCAG
    AGTTTAGAGA GCCAAGAACT TAAGTCCATT GTACATGATC TTTTGACTAC AGTAGAAGAA
    CTCTGTGACC AGAATGAATT TCATGGTTCT GAGGAGAGAT TTTTTGAGCT GGTGGAAAGA
    TGCGCTGATC AGAGACCGGA ATCTTCCGTA TTAAACTTGA TAGCATACAG AGCTCAATCC
    ATTCATCCTG CCAAAGATGG CTGGATTCAC AACCTGCAGA TGTTAATGGA GAGATTCTTC
    AGGAACGAAA GTCGTAGTGC TGTTCGTATT AAAGTTCTGG ATGTCTTGTC CTTTGTACTG
    AGTATTAACA GACAGTTCTA TGAGGAAGAG CTGATAAACT TGGTTGTGAT CTCTCAGCTG
    GCTCACATTC CAGAGGATAA AGACCATCAA GTTCGAAAAC TTGCCACTCA GCTACTTGTA
    GACCTTGCTG AAGGCTGCAA CACACATCAC TTCAACAGCT TGCTTGATAT CATAGAAAAG
    GTGGCTGCGC ATTCTCTCTC ATCTCCATCT GAACTGGAAG AGAGGGATTT GCTATCATAT
    TCAGCTTCTT TGGAGGACGT GAAGACAGCA GTTCTTGGAC TTCTGATAAT TCTTCAGACA
    AAACTCTACA GCTTACCTTC CAGCCATGCC ATGCGGGTGT ATGAGATGCT GATTCACCAC
    ATCCAGCGCC ACTATATGTA TGCGTACAGC CTTGCTGTTG CTAGCAGCAT CAGACTGCAG
    GTGTTTGATT TCCTCCTGAT GCTTCGAGCT GACTCCCTCC ACCGTCTTGG CCTTTCTAAC
    AAGGATGGGG CAGTGAGATT CAGCCCTTAC TGTCTGTGTG ATTTTGTAGA AGCAGAGAAG
    AGAGCTTCTG AGAAAAAGCC TACTGGTACG CTCTCTCCAC CTTCAGGAAG TCCCAGTGTG
    CCTTCCCAGA ACAGCACCAT TCGAATAGGG CATTTGCCAT ACTCAATGGT CTTTGGTGTC
    CTTCTGCAGT GTTTGAAGCA AGAGACAGAC TGGAAGGTGT TGAAGTTGGT CCTCAACAAA
    CTACCAGAAT CACTCCGCTA TAAAGTGCTG TTTTTAACCT CTCCTTGTAA CATAGACCTC
    CTGGCATCTG CACTGAGCTA CATGCTCACA GACAAGAAGA CAACCGACAG GCTCCATGGT
    ACCCCAGAAG GCTTTTCCCG CACCGATTTG CACCTGGCTG TAGTTCCAGT ACTGACAGCG
    TTGATATCTT ATCATAATTA TCTGGACAAA GCTAAACAGC GTGAAATAGT GTACTGCCTG
    GAGCATGGCC TTATTTATCG CTGTGCAAAC CAGTGCGTTG TGGCACTCTC TGTCTGCAGC
    GTTGAGATGC CCGACATCAT CATAAAGGCA CTTCCAGTCT TAATAGTCAA ATTAACCCAC
    ATATCTGCTA CAGCCAACAT GGCAATCCCT CTCTTAGAAT TTCTTTCAAC TCTAGCAAGG
    CTGCCTCACC TCTACAGGAA CTTTGCTGCA GAGCAGTATG CCAGTGTGTT TGCCATCTCC
    CTGCCATACA CAAACCCTTC CAAGTTTAAC CAGTACATTG TTTGCCTGGC CCACCACGTG
    ATAGCTATGT GGTTCATTCG GTGTCGTCTT CCCTTCCGAA AGGACTTTGT TCCCTATATT
    ACAAAGGGTT TGCGCTCCAA TGTGCTGCTC TCCTTTGATG ACACACCTGA GAAGGACAGT
    TTCAGGGCTC GTAGTACAAG CCTCAATGAG AGGCCAAAAA GTAGTTTAAG ACTAGCCAAA
    AATGCAAAGC AAGGCTTGAA TAACTCTCCT CCAGTGAAAG AGCTGAAAGA ACCCTCTGCA
    GTTGATGCCT TCCGATCCCG CAGCATCAGT GTGTCTGAAC ATGTGGTCCG CAGTCGGATA
    CAGACATCCA TCACTAGTTC CAGCTTGGGC TCTGCAGATG AAAATTCAAT GGCTCAGGCT
    GATGACAACT TAAAAAATCT CCATCTGGAA CTCACTGAGA CTTGTCTGGA TATGATGGCC
    CGATACGTCT TTTCCAACTT CACTGCGGTG CCTAAGAGGT CTCCTGTGGG TGAGTTTCTG
    TTGGCTGGAG GAAGGACAAA GACATGGCTG GTTGGCAACA AGTTGGTGAC CATCACTACA
    AGTGTCGGAA CAGGGACAAG GTCCTTGCTT GGCCTGGACT CTGGAGATCT CCAAAGCAGC
    ACAGAATCAG GCTCAGACCC GGTTTTGCAA GTGAGACAAA CTAAAGAAGC TCCAGCAAAG
    CTCGAATCTC AAGCTGGGCA GCAGGTTTGC AGAAGCTCTC GCAACAGAGT CAGATCCATG
    TCTGGTGGTC ATGCCTTACG TGTTGGTGCC TTAGACAGCA CAGCCTCCCA CTTCCCTGGT
    GGCTCGGCTT CACAAGGGAC ACAGAGTCCT CCTGCTCCTG CACCTCGGTC AGAGAAGACT
    AATCCTGCTC CACAGACACC TCTGCAGAAA GAAAAAGCGA ACTTAGCTGC ATATGTCCCA
    CTGCTGACAC AGGGCTGGGC AGAAATCCTT GTGCGCAGAC CCACAGGTAA CACAAGCTGG
    CTAATGAGTC TGGAGAACCC GCTCAGTCCC TTTTCTTCAG ACATTAACAA TATGCCCCTG
    CAAGAGCTGT CCAACGCACT CATGGCTGCA GAGCGTTTTA AAGAGCACCG AGAAACAGCT
    CTCTACAAAT CCCTGTCTGT CCCTTCCTCC AGCTTGGCCA CTGGGACAGC CAAGCCTTCA
    CTTCTCCAGC GTTCCAACAC AGTGGCCTCT TTCTCTTCCA TGTACCAGTC CAGTTGTCAA
    GGGAAGTTGC ACAGGAGCAT ATCCTGGGCA GAGTCTGCGG TCGTGCTGGA GGAAGGGAGC
    CCCTTGGAAA TGGAGCTGAA GGAAACTGAG TCCCCAGCTG AGTCTCCAGA GAGTGAAGAT
    TTTGAGACGT CGTGTTCTGA GCAAGTCCTG AGCGAGGAAA GGTTTGGTAA AGCCCAGGAG
    TTTTACAGCA GGTCGTCCTC GACCTCTAGC CAAGATGAAA AATCTCTGAA GTCGGACGAG
    CTGGTGGAGG GCGGTATTCC CATCGGGAGA GTGCTGCCAG CAGAGGATGG CCGGGCACTG
    GAGGAGCTCT CCTTCCAGCC CTCCCAGCCG CTCAGCAAAT CGAGCTCTTC CCCCGAGCTG
    CAGACTTTGC AGGAGATCCT CAAGGATGCA AATGGCAGAG AAGTAACAAG GAGGCTGAGC
    ACGGAAGTGA AGTCCAAATC TCAGTCTGGG AACCTGGAAG GGGAAGGACT GGGCAGTTGG
    CTGAACAAGG GTGAGGATGC CAGAGCAGCA GGCTCGGGGG GGTTGGATGG CAGTGGCCCC
    ACCACCTCGC CACGCTCCCC ATCTGGGCAC CGGCCCCGGG GGTACACCAT CTCTGACTCT
    GCCCCATCAC GGAGAGGGAA GAGGATGGAG AGAGATGCCT TCAAGAGCAG AACAGCAGCC
    ACCAATGCTG AGAAAGTACC TGGAATAAAC CCAAGCTTTG TGTTTTTACA ATTGTACCAT
    TCCCCGTTCT TTGGTGATGA AAACAACAAG CCCCTCCTGT TGCCAAATGA GACGTTCGAA
    AAATCGGTGC AGCTCCTGGA TCAAATTCCT TCGTATGACA CCCACAAGAT CGCAGTGCTG
    TACGTTGGAG AGGGCCAGAG CAACAATGAA ATCGCTATTC TGTCCAATGA ACACGGCTCC
    TATCGCTACA CCGAGTTCCT GACAGGCTTA GGGAAGCTCA TTGAGCTCAA AGACTGCCAG
    CCAGATAAAA TCTACCTCGG TGGCCTGGAC GTGTGTGGGG AGGATGGACA GTTTACCTAC
    TGCTGGCACG ATGACATCAT GCAAGCTATT TTCCACATTG CTACACTGAT GCCCACAAAG
    GATTTGGATA AATATCGCTG TGACAAGAAG AGGCACCTTG GGAATGATTT TGTTTCTATT
    GTTTACAATG ATTCTGGTGA GGACTTCAAA CTGGGAACAA TAAAGGGTCA GTTCAATTTT
    GTGCATGTTA TCATAACCCC ACTTGACTAT GACTGCAACC TGGTCACACT GCAGTGTCGA
    AAAGACATGG AGGGCCTTGT AGACACGAGT GTTGCTAAGA TCATCTCTGA CAAGAACCTG
    CCATTCGTGG CCCGTCAGAT GGCCCTGCAT GCTAACATGG CTTCCCAGGT GCACCACAGT
    CGATCCAACC CTACTGACAC CTACCCATCC AAATGGATCG CTCGGCTGCG GCACATCAAA
    CGGCTGAGGC ACCGGCTACG TGAAGAAACC CAGTACCAAA CTCCTGGCCT TCCCCTGCAG
    ATGCACCCGT CTGCTCCAAC AAAGCCCTCT CCCCAGATGG CTCAGGACCC ACCGCCGGCC
    TACGAGACCG GTCAGAGGAA GCGGCTGATC TCCTCGGTGG ATGACTTCAC TGAGTTTGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42375
    Clone ID Related Accession (Same CDS sequence) XM_015294449.2 , XM_015294449.1
    Accession Version XM_015294449.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tuberin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TAGTTTAAGA CTAGCCAAAA ATGCAAAGCA AGGCTTGAAT
    AACTCTCCTC CAGTGAAAGA GCTGAAAGAA CCCTCTGCAG TTGATGCCTT CCGATCCCGC
    AGCATCAGTG TGTCTGAACA TGTGGTCCGC AGTCGGATAC AGACATCCAT CACTAGTTCC
    AGCTTGGGCT CTGCAGATGA AAATTCAATG GCTCAGGCTG ATGACAACTT AAAAAATCTC
    CATCTGGAAC TCACTGAGAC TTGTCTGGAT ATGATGGCCC GATACGTCTT TTCCAACTTC
    ACTGCGGTGC CTAAGAGGTC TCCTGTGGGT GAGTTTCTGT TGGCTGGAGG AAGGACAAAG
    ACATGGCTGG TTGGCAACAA GTTGGTGACC ATCACTACAA GTGTCGGAAC AGGGACAAGG
    TCCTTGCTTG GCCTGGACTC TGGAGATCTC CAAAGCAGCA CAGAATCAGG CTCAGACCCG
    GTTTTGCAAG TGAGACAAAC TAAAGAAGCT CCAGCAAAGC TCGAATCTCA AGCTGGGCAG
    CAGGTTTGCA GAAGCTCTCG CAACAGAGTC AGATCCATGT CTGGTGGTCA TGCCTTACGT
    GTTGGTGCCT TAGACAGCAC AGCCTCCCAC TTCCCTGGTG GCTCGGCTTC ACAAGGGACA
    CAGAGTCCTC CTGCTCCTGC ACCTCGGTCA GAGAAGACTA ATCCTGCTCC ACAGACACCT
    CTGCAGAAAG AAAAAGCGAA CTTAGCTGCA TATGTCCCAC TGCTGACACA GGGCTGGGCA
    GAAATCCTTG TGCGCAGACC CACAGGTAAC ACAAGCTGGC TAATGAGTCT GGAGAACCCG
    CTCAGTCCCT TTTCTTCAGA CATTAACAAT ATGCCCCTGC AAGAGCTGTC CAACGCACTC
    ATGGCTGCAG AGCGTTTTAA AGAGCACCGA GAAACAGCTC TCTACAAATC CCTGTCTGTC
    CCTTCCTCCA GCTTGGCCAC TGGGACAGCC AAGCCTTCAC TTCTCCAGCG TTCCAACACA
    GAGTCTGCGG TCGTGCTGGA GGAAGGGAGC CCCTTGGAAA TGGAGCTGAA GGAAACTGAG
    TCCCCAGCTG AGTCTCCAGA GAGTGAAGAT TTTGAGACGT CGTGTTCTGA GCAAGTCCTG
    AGCGAGGAAA GGTTTGGTAA AGCCCAGGAG TTTTACAGCA GGTCGTCCTC GACCTCTAGC
    CAAGATGAAA AATCTCTGAA GTCGGACGAG CTGGTGGAGG GCGGTATTCC CATCGGGAGA
    GTGCTGCCAG CAGAGGATGG CCGGGCACTG GAGGAGCTCT CCTTCCAGCC CTCCCAGCCG
    CTCAGCAAAT CGAGCTCTTC CCCCGAGCTG CAGACTTTGC AGGAGATCCT CAAGGATGCA
    AATGGCAGAG AAGTAACAAG GAGGCTGAGC ACGGAAGTGA AGTCCAAATC TCAGTCTGGG
    AACCTGGAAG GGGAAGGACT GGGCAGTTGG CTGAACAAGG GTGAGGATGC CAGAGCAGCA
    GGCTCGGGGG GGTTGGATGG CAGTGGCCCC ACCACCTCGC CACGCTCCCC ATCTGGGCAC
    CGGCCCCGGG GGTACACCAT CTCTGACTCT GCCCCATCAC GGAGAGGGAA GAGGATGGAG
    AGAGATGCCT TCAAGAGCAG AACAGCAGCC ACCAATGCTG AGAAAGTACC TGGAATAAAC
    CCAAGCTTTG TGTTTTTACA ATTGTACCAT TCCCCGTTCT TTGGTGATGA AAACAACAAG
    CCCCTCCTGT TGCCAAATGA GACGTTCGAA AAATCGGTGC AGCTCCTGGA TCAAATTCCT
    TCGTATGACA CCCACAAGAT CGCAGTGCTG TACGTTGGAG AGGGCCAGAG CAACAATGAA
    ATCGCTATTC TGTCCAATGA ACACGGCTCC TATCGCTACA CCGAGTTCCT GACAGGCTTA
    GGGAAGCTCA TTGAGCTCAA AGACTGCCAG CCAGATAAAA TCTACCTCGG TGGCCTGGAC
    GTGTGTGGGG AGGATGGACA GTTTACCTAC TGCTGGCACG ATGACATCAT GCAAGCTATT
    TTCCACATTG CTACACTGAT GCCCACAAAG GATTTGGATA AATATCGCTG TGACAAGAAG
    AGGCACCTTG GGAATGATTT TGTTTCTATT GTTTACAATG ATTCTGGTGA GGACTTCAAA
    CTGGGAACAA TAAAGGGTCA GTTCAATTTT GTGCATGTTA TCATAACCCC ACTTGACTAT
    GACTGCAACC TGGTCACACT GCAGTGTCGA AAAGACATGG AGGGCCTTGT AGACACGAGT
    GTTGCTAAGA TCATCTCTGA CAAGAACCTG CCATTCGTGG CCCGTCAGAT GGCCCTGCAT
    GCTAACATGG CTTCCCAGGT GCACCACAGT CGATCCAACC CTACTGACAC CTACCCATCC
    AAATGGATCG CTCGGCTGCG GCACATCAAA CGGCTGAGGC ACCGGCTACG TGAAGAAACC
    CAGTACCAAA CTCCTGGCCT TCCCCTGCAG ATGCACCCGT CTGCTCCAAC AAAGCCCTCT
    CCCCAGATGG CTCAGGACCC ACCGCCGGCC TACGAGACCG GTCAGAGGAA GCGGCTGATC
    TCCTCGGTGG ATGACTTCAC TGAGTTTGTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149935.1
    CDS58..5490
    Translation

    Target ORF information:

    RefSeq Version XM_015294449.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294449.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TAGTTTAAGA CTAGCCAAAA ATGCAAAGCA AGGCTTGAAT
    AACTCTCCTC CAGTGAAAGA GCTGAAAGAA CCCTCTGCAG TTGATGCCTT CCGATCCCGC
    AGCATCAGTG TGTCTGAACA TGTGGTCCGC AGTCGGATAC AGACATCCAT CACTAGTTCC
    AGCTTGGGCT CTGCAGATGA AAATTCAATG GCTCAGGCTG ATGACAACTT AAAAAATCTC
    CATCTGGAAC TCACTGAGAC TTGTCTGGAT ATGATGGCCC GATACGTCTT TTCCAACTTC
    ACTGCGGTGC CTAAGAGGTC TCCTGTGGGT GAGTTTCTGT TGGCTGGAGG AAGGACAAAG
    ACATGGCTGG TTGGCAACAA GTTGGTGACC ATCACTACAA GTGTCGGAAC AGGGACAAGG
    TCCTTGCTTG GCCTGGACTC TGGAGATCTC CAAAGCAGCA CAGAATCAGG CTCAGACCCG
    GTTTTGCAAG TGAGACAAAC TAAAGAAGCT CCAGCAAAGC TCGAATCTCA AGCTGGGCAG
    CAGGTTTGCA GAAGCTCTCG CAACAGAGTC AGATCCATGT CTGGTGGTCA TGCCTTACGT
    GTTGGTGCCT TAGACAGCAC AGCCTCCCAC TTCCCTGGTG GCTCGGCTTC ACAAGGGACA
    CAGAGTCCTC CTGCTCCTGC ACCTCGGTCA GAGAAGACTA ATCCTGCTCC ACAGACACCT
    CTGCAGAAAG AAAAAGCGAA CTTAGCTGCA TATGTCCCAC TGCTGACACA GGGCTGGGCA
    GAAATCCTTG TGCGCAGACC CACAGGTAAC ACAAGCTGGC TAATGAGTCT GGAGAACCCG
    CTCAGTCCCT TTTCTTCAGA CATTAACAAT ATGCCCCTGC AAGAGCTGTC CAACGCACTC
    ATGGCTGCAG AGCGTTTTAA AGAGCACCGA GAAACAGCTC TCTACAAATC CCTGTCTGTC
    CCTTCCTCCA GCTTGGCCAC TGGGACAGCC AAGCCTTCAC TTCTCCAGCG TTCCAACACA
    GAGTCTGCGG TCGTGCTGGA GGAAGGGAGC CCCTTGGAAA TGGAGCTGAA GGAAACTGAG
    TCCCCAGCTG AGTCTCCAGA GAGTGAAGAT TTTGAGACGT CGTGTTCTGA GCAAGTCCTG
    AGCGAGGAAA GGTTTGGTAA AGCCCAGGAG TTTTACAGCA GGTCGTCCTC GACCTCTAGC
    CAAGATGAAA AATCTCTGAA GTCGGACGAG CTGGTGGAGG GCGGTATTCC CATCGGGAGA
    GTGCTGCCAG CAGAGGATGG CCGGGCACTG GAGGAGCTCT CCTTCCAGCC CTCCCAGCCG
    CTCAGCAAAT CGAGCTCTTC CCCCGAGCTG CAGACTTTGC AGGAGATCCT CAAGGATGCA
    AATGGCAGAG AAGTAACAAG GAGGCTGAGC ACGGAAGTGA AGTCCAAATC TCAGTCTGGG
    AACCTGGAAG GGGAAGGACT GGGCAGTTGG CTGAACAAGG GTGAGGATGC CAGAGCAGCA
    GGCTCGGGGG GGTTGGATGG CAGTGGCCCC ACCACCTCGC CACGCTCCCC ATCTGGGCAC
    CGGCCCCGGG GGTACACCAT CTCTGACTCT GCCCCATCAC GGAGAGGGAA GAGGATGGAG
    AGAGATGCCT TCAAGAGCAG AACAGCAGCC ACCAATGCTG AGAAAGTACC TGGAATAAAC
    CCAAGCTTTG TGTTTTTACA ATTGTACCAT TCCCCGTTCT TTGGTGATGA AAACAACAAG
    CCCCTCCTGT TGCCAAATGA GACGTTCGAA AAATCGGTGC AGCTCCTGGA TCAAATTCCT
    TCGTATGACA CCCACAAGAT CGCAGTGCTG TACGTTGGAG AGGGCCAGAG CAACAATGAA
    ATCGCTATTC TGTCCAATGA ACACGGCTCC TATCGCTACA CCGAGTTCCT GACAGGCTTA
    GGGAAGCTCA TTGAGCTCAA AGACTGCCAG CCAGATAAAA TCTACCTCGG TGGCCTGGAC
    GTGTGTGGGG AGGATGGACA GTTTACCTAC TGCTGGCACG ATGACATCAT GCAAGCTATT
    TTCCACATTG CTACACTGAT GCCCACAAAG GATTTGGATA AATATCGCTG TGACAAGAAG
    AGGCACCTTG GGAATGATTT TGTTTCTATT GTTTACAATG ATTCTGGTGA GGACTTCAAA
    CTGGGAACAA TAAAGGGTCA GTTCAATTTT GTGCATGTTA TCATAACCCC ACTTGACTAT
    GACTGCAACC TGGTCACACT GCAGTGTCGA AAAGACATGG AGGGCCTTGT AGACACGAGT
    GTTGCTAAGA TCATCTCTGA CAAGAACCTG CCATTCGTGG CCCGTCAGAT GGCCCTGCAT
    GCTAACATGG CTTCCCAGGT GCACCACAGT CGATCCAACC CTACTGACAC CTACCCATCC
    AAATGGATCG CTCGGCTGCG GCACATCAAA CGGCTGAGGC ACCGGCTACG TGAAGAAACC
    CAGTACCAAA CTCCTGGCCT TCCCCTGCAG ATGCACCCGT CTGCTCCAAC AAAGCCCTCT
    CCCCAGATGG CTCAGGACCC ACCGCCGGCC TACGAGACCG GTCAGAGGAA GCGGCTGATC
    TCCTCGGTGG ATGACTTCAC TGAGTTTGTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42375
    Clone ID Related Accession (Same CDS sequence) XM_015294449.2 , XM_015294449.1
    Accession Version XM_015294449.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tuberin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TAGTTTAAGA CTAGCCAAAA ATGCAAAGCA AGGCTTGAAT
    AACTCTCCTC CAGTGAAAGA GCTGAAAGAA CCCTCTGCAG TTGATGCCTT CCGATCCCGC
    AGCATCAGTG TGTCTGAACA TGTGGTCCGC AGTCGGATAC AGACATCCAT CACTAGTTCC
    AGCTTGGGCT CTGCAGATGA AAATTCAATG GCTCAGGCTG ATGACAACTT AAAAAATCTC
    CATCTGGAAC TCACTGAGAC TTGTCTGGAT ATGATGGCCC GATACGTCTT TTCCAACTTC
    ACTGCGGTGC CTAAGAGGTC TCCTGTGGGT GAGTTTCTGT TGGCTGGAGG AAGGACAAAG
    ACATGGCTGG TTGGCAACAA GTTGGTGACC ATCACTACAA GTGTCGGAAC AGGGACAAGG
    TCCTTGCTTG GCCTGGACTC TGGAGATCTC CAAAGCAGCA CAGAATCAGG CTCAGACCCG
    GTTTTGCAAG TGAGACAAAC TAAAGAAGCT CCAGCAAAGC TCGAATCTCA AGCTGGGCAG
    CAGGTTTGCA GAAGCTCTCG CAACAGAGTC AGATCCATGT CTGGTGGTCA TGCCTTACGT
    GTTGGTGCCT TAGACAGCAC AGCCTCCCAC TTCCCTGGTG GCTCGGCTTC ACAAGGGACA
    CAGAGTCCTC CTGCTCCTGC ACCTCGGTCA GAGAAGACTA ATCCTGCTCC ACAGACACCT
    CTGCAGAAAG AAAAAGCGAA CTTAGCTGCA TATGTCCCAC TGCTGACACA GGGCTGGGCA
    GAAATCCTTG TGCGCAGACC CACAGGTAAC ACAAGCTGGC TAATGAGTCT GGAGAACCCG
    CTCAGTCCCT TTTCTTCAGA CATTAACAAT ATGCCCCTGC AAGAGCTGTC CAACGCACTC
    ATGGCTGCAG AGCGTTTTAA AGAGCACCGA GAAACAGCTC TCTACAAATC CCTGTCTGTC
    CCTTCCTCCA GCTTGGCCAC TGGGACAGCC AAGCCTTCAC TTCTCCAGCG TTCCAACACA
    GAGTCTGCGG TCGTGCTGGA GGAAGGGAGC CCCTTGGAAA TGGAGCTGAA GGAAACTGAG
    TCCCCAGCTG AGTCTCCAGA GAGTGAAGAT TTTGAGACGT CGTGTTCTGA GCAAGTCCTG
    AGCGAGGAAA GGTTTGGTAA AGCCCAGGAG TTTTACAGCA GGTCGTCCTC GACCTCTAGC
    CAAGATGAAA AATCTCTGAA GTCGGACGAG CTGGTGGAGG GCGGTATTCC CATCGGGAGA
    GTGCTGCCAG CAGAGGATGG CCGGGCACTG GAGGAGCTCT CCTTCCAGCC CTCCCAGCCG
    CTCAGCAAAT CGAGCTCTTC CCCCGAGCTG CAGACTTTGC AGGAGATCCT CAAGGATGCA
    AATGGCAGAG AAGTAACAAG GAGGCTGAGC ACGGAAGTGA AGTCCAAATC TCAGTCTGGG
    AACCTGGAAG GGGAAGGACT GGGCAGTTGG CTGAACAAGG GTGAGGATGC CAGAGCAGCA
    GGCTCGGGGG GGTTGGATGG CAGTGGCCCC ACCACCTCGC CACGCTCCCC ATCTGGGCAC
    CGGCCCCGGG GGTACACCAT CTCTGACTCT GCCCCATCAC GGAGAGGGAA GAGGATGGAG
    AGAGATGCCT TCAAGAGCAG AACAGCAGCC ACCAATGCTG AGAAAGTACC TGGAATAAAC
    CCAAGCTTTG TGTTTTTACA ATTGTACCAT TCCCCGTTCT TTGGTGATGA AAACAACAAG
    CCCCTCCTGT TGCCAAATGA GACGTTCGAA AAATCGGTGC AGCTCCTGGA TCAAATTCCT
    TCGTATGACA CCCACAAGAT CGCAGTGCTG TACGTTGGAG AGGGCCAGAG CAACAATGAA
    ATCGCTATTC TGTCCAATGA ACACGGCTCC TATCGCTACA CCGAGTTCCT GACAGGCTTA
    GGGAAGCTCA TTGAGCTCAA AGACTGCCAG CCAGATAAAA TCTACCTCGG TGGCCTGGAC
    GTGTGTGGGG AGGATGGACA GTTTACCTAC TGCTGGCACG ATGACATCAT GCAAGCTATT
    TTCCACATTG CTACACTGAT GCCCACAAAG GATTTGGATA AATATCGCTG TGACAAGAAG
    AGGCACCTTG GGAATGATTT TGTTTCTATT GTTTACAATG ATTCTGGTGA GGACTTCAAA
    CTGGGAACAA TAAAGGGTCA GTTCAATTTT GTGCATGTTA TCATAACCCC ACTTGACTAT
    GACTGCAACC TGGTCACACT GCAGTGTCGA AAAGACATGG AGGGCCTTGT AGACACGAGT
    GTTGCTAAGA TCATCTCTGA CAAGAACCTG CCATTCGTGG CCCGTCAGAT GGCCCTGCAT
    GCTAACATGG CTTCCCAGGT GCACCACAGT CGATCCAACC CTACTGACAC CTACCCATCC
    AAATGGATCG CTCGGCTGCG GCACATCAAA CGGCTGAGGC ACCGGCTACG TGAAGAAACC
    CAGTACCAAA CTCCTGGCCT TCCCCTGCAG ATGCACCCGT CTGCTCCAAC AAAGCCCTCT
    CCCCAGATGG CTCAGGACCC ACCGCCGGCC TACGAGACCG GTCAGAGGAA GCGGCTGATC
    TCCTCGGTGG ATGACTTCAC TGAGTTTGTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149935.1
    CDS58..5490
    Translation

    Target ORF information:

    RefSeq Version XM_015294449.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TAGTTTAAGA CTAGCCAAAA ATGCAAAGCA AGGCTTGAAT
    AACTCTCCTC CAGTGAAAGA GCTGAAAGAA CCCTCTGCAG TTGATGCCTT CCGATCCCGC
    AGCATCAGTG TGTCTGAACA TGTGGTCCGC AGTCGGATAC AGACATCCAT CACTAGTTCC
    AGCTTGGGCT CTGCAGATGA AAATTCAATG GCTCAGGCTG ATGACAACTT AAAAAATCTC
    CATCTGGAAC TCACTGAGAC TTGTCTGGAT ATGATGGCCC GATACGTCTT TTCCAACTTC
    ACTGCGGTGC CTAAGAGGTC TCCTGTGGGT GAGTTTCTGT TGGCTGGAGG AAGGACAAAG
    ACATGGCTGG TTGGCAACAA GTTGGTGACC ATCACTACAA GTGTCGGAAC AGGGACAAGG
    TCCTTGCTTG GCCTGGACTC TGGAGATCTC CAAAGCAGCA CAGAATCAGG CTCAGACCCG
    GTTTTGCAAG TGAGACAAAC TAAAGAAGCT CCAGCAAAGC TCGAATCTCA AGCTGGGCAG
    CAGGTTTGCA GAAGCTCTCG CAACAGAGTC AGATCCATGT CTGGTGGTCA TGCCTTACGT
    GTTGGTGCCT TAGACAGCAC AGCCTCCCAC TTCCCTGGTG GCTCGGCTTC ACAAGGGACA
    CAGAGTCCTC CTGCTCCTGC ACCTCGGTCA GAGAAGACTA ATCCTGCTCC ACAGACACCT
    CTGCAGAAAG AAAAAGCGAA CTTAGCTGCA TATGTCCCAC TGCTGACACA GGGCTGGGCA
    GAAATCCTTG TGCGCAGACC CACAGGTAAC ACAAGCTGGC TAATGAGTCT GGAGAACCCG
    CTCAGTCCCT TTTCTTCAGA CATTAACAAT ATGCCCCTGC AAGAGCTGTC CAACGCACTC
    ATGGCTGCAG AGCGTTTTAA AGAGCACCGA GAAACAGCTC TCTACAAATC CCTGTCTGTC
    CCTTCCTCCA GCTTGGCCAC TGGGACAGCC AAGCCTTCAC TTCTCCAGCG TTCCAACACA
    GAGTCTGCGG TCGTGCTGGA GGAAGGGAGC CCCTTGGAAA TGGAGCTGAA GGAAACTGAG
    TCCCCAGCTG AGTCTCCAGA GAGTGAAGAT TTTGAGACGT CGTGTTCTGA GCAAGTCCTG
    AGCGAGGAAA GGTTTGGTAA AGCCCAGGAG TTTTACAGCA GGTCGTCCTC GACCTCTAGC
    CAAGATGAAA AATCTCTGAA GTCGGACGAG CTGGTGGAGG GCGGTATTCC CATCGGGAGA
    GTGCTGCCAG CAGAGGATGG CCGGGCACTG GAGGAGCTCT CCTTCCAGCC CTCCCAGCCG
    CTCAGCAAAT CGAGCTCTTC CCCCGAGCTG CAGACTTTGC AGGAGATCCT CAAGGATGCA
    AATGGCAGAG AAGTAACAAG GAGGCTGAGC ACGGAAGTGA AGTCCAAATC TCAGTCTGGG
    AACCTGGAAG GGGAAGGACT GGGCAGTTGG CTGAACAAGG GTGAGGATGC CAGAGCAGCA
    GGCTCGGGGG GGTTGGATGG CAGTGGCCCC ACCACCTCGC CACGCTCCCC ATCTGGGCAC
    CGGCCCCGGG GGTACACCAT CTCTGACTCT GCCCCATCAC GGAGAGGGAA GAGGATGGAG
    AGAGATGCCT TCAAGAGCAG AACAGCAGCC ACCAATGCTG AGAAAGTACC TGGAATAAAC
    CCAAGCTTTG TGTTTTTACA ATTGTACCAT TCCCCGTTCT TTGGTGATGA AAACAACAAG
    CCCCTCCTGT TGCCAAATGA GACGTTCGAA AAATCGGTGC AGCTCCTGGA TCAAATTCCT
    TCGTATGACA CCCACAAGAT CGCAGTGCTG TACGTTGGAG AGGGCCAGAG CAACAATGAA
    ATCGCTATTC TGTCCAATGA ACACGGCTCC TATCGCTACA CCGAGTTCCT GACAGGCTTA
    GGGAAGCTCA TTGAGCTCAA AGACTGCCAG CCAGATAAAA TCTACCTCGG TGGCCTGGAC
    GTGTGTGGGG AGGATGGACA GTTTACCTAC TGCTGGCACG ATGACATCAT GCAAGCTATT
    TTCCACATTG CTACACTGAT GCCCACAAAG GATTTGGATA AATATCGCTG TGACAAGAAG
    AGGCACCTTG GGAATGATTT TGTTTCTATT GTTTACAATG ATTCTGGTGA GGACTTCAAA
    CTGGGAACAA TAAAGGGTCA GTTCAATTTT GTGCATGTTA TCATAACCCC ACTTGACTAT
    GACTGCAACC TGGTCACACT GCAGTGTCGA AAAGACATGG AGGGCCTTGT AGACACGAGT
    GTTGCTAAGA TCATCTCTGA CAAGAACCTG CCATTCGTGG CCCGTCAGAT GGCCCTGCAT
    GCTAACATGG CTTCCCAGGT GCACCACAGT CGATCCAACC CTACTGACAC CTACCCATCC
    AAATGGATCG CTCGGCTGCG GCACATCAAA CGGCTGAGGC ACCGGCTACG TGAAGAAACC
    CAGTACCAAA CTCCTGGCCT TCCCCTGCAG ATGCACCCGT CTGCTCCAAC AAAGCCCTCT
    CCCCAGATGG CTCAGGACCC ACCGCCGGCC TACGAGACCG GTCAGAGGAA GCGGCTGATC
    TCCTCGGTGG ATGACTTCAC TGAGTTTGTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42376
    Clone ID Related Accession (Same CDS sequence) XM_015294450.2 , XM_015294450.1
    Accession Version XM_015294450.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tuberin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TTTAAGACTA GCCAAAAATG CAAAGCAAGG CTTGAATAAC
    TCTCCTCCAG TGAAAGAGCT GAAAGAACCC TCTGCAGTTG ATGCCTTCCG ATCCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGT CGGATACAGA CATCCATCAC TAGTTCCAGC
    TTGGGCTCTG CAGATGAAAA TTCAATGGCT CAGGCTGATG ACAACTTAAA AAATCTCCAT
    CTGGAACTCA CTGAGACTTG TCTGGATATG ATGGCCCGAT ACGTCTTTTC CAACTTCACT
    GCGGTGCCTA AGAGGTCTCC TGTGGGTGAG TTTCTGTTGG CTGGAGGAAG GACAAAGACA
    TGGCTGGTTG GCAACAAGTT GGTGACCATC ACTACAAGTG TCGGAACAGG GACAAGGTCC
    TTGCTTGGCC TGGACTCTGG AGATCTCCAA AGCAGCACAG AATCAGGCTC AGACCCGGTT
    TTGCAAGTGA GACAAACTAA AGAAGCTCCA GCAAAGCTCG AATCTCAAGC TGGGCAGCAG
    GTTTGCAGAA GCTCTCGCAA CAGAGTCAGA TCCATGTCTG GTGGTCATGC CTTACGTGTT
    GGTGCCTTAG ACAGCACAGC CTCCCACTTC CCTGGTGGCT CGGCTTCACA AGGGACACAG
    AGTCCTCCTG CTCCTGCACC TCGGTCAGAG AAGACTAATC CTGCTCCACA GACACCTCTG
    CAGAAAGAAA AAGCGAACTT AGCTGCATAT GTCCCACTGC TGACACAGGG CTGGGCAGAA
    ATCCTTGTGC GCAGACCCAC AGGTAACACA AGCTGGCTAA TGAGTCTGGA GAACCCGCTC
    AGTCCCTTTT CTTCAGACAT TAACAATATG CCCCTGCAAG AGCTGTCCAA CGCACTCATG
    GCTGCAGAGC GTTTTAAAGA GCACCGAGAA ACAGCTCTCT ACAAATCCCT GTCTGTCCCT
    TCCTCCAGCT TGGCCACTGG GACAGCCAAG CCTTCACTTC TCCAGCGTTC CAACACAGAG
    TCTGCGGTCG TGCTGGAGGA AGGGAGCCCC TTGGAAATGG AGCTGAAGGA AACTGAGTCC
    CCAGCTGAGT CTCCAGAGAG TGAAGATTTT GAGACGTCGT GTTCTGAGCA AGTCCTGAGC
    GAGGAAAGGT TTGGTAAAGC CCAGGAGTTT TACAGCAGGT CGTCCTCGAC CTCTAGCCAA
    GATGAAAAAT CTCTGAAGTC GGACGAGCTG GTGGAGGGCG GTATTCCCAT CGGGAGAGTG
    CTGCCAGCAG AGGATGGCCG GGCACTGGAG GAGCTCTCCT TCCAGCCCTC CCAGCCGCTC
    AGCAAATCGA GCTCTTCCCC CGAGCTGCAG ACTTTGCAGG AGATCCTCAA GGATGCAAAT
    GGCAGAGAAG TAACAAGGAG GCTGAGCACG GAAGTGAAGT CCAAATCTCA GTCTGGGAAC
    CTGGAAGGGG AAGGACTGGG CAGTTGGCTG AACAAGGGTG AGGATGCCAG AGCAGCAGGC
    TCGGGGGGGT TGGATGGCAG TGGCCCCACC ACCTCGCCAC GCTCCCCATC TGGGCACCGG
    CCCCGGGGGT ACACCATCTC TGACTCTGCC CCATCACGGA GAGGGAAGAG GATGGAGAGA
    GATGCCTTCA AGAGCAGAAC AGCAGCCACC AATGCTGAGA AAGTACCTGG AATAAACCCA
    AGCTTTGTGT TTTTACAATT GTACCATTCC CCGTTCTTTG GTGATGAAAA CAACAAGCCC
    CTCCTGTTGC CAAATGAGAC GTTCGAAAAA TCGGTGCAGC TCCTGGATCA AATTCCTTCG
    TATGACACCC ACAAGATCGC AGTGCTGTAC GTTGGAGAGG GCCAGAGCAA CAATGAAATC
    GCTATTCTGT CCAATGAACA CGGCTCCTAT CGCTACACCG AGTTCCTGAC AGGCTTAGGG
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCA GATAAAATCT ACCTCGGTGG CCTGGACGTG
    TGTGGGGAGG ATGGACAGTT TACCTACTGC TGGCACGATG ACATCATGCA AGCTATTTTC
    CACATTGCTA CACTGATGCC CACAAAGGAT TTGGATAAAT ATCGCTGTGA CAAGAAGAGG
    CACCTTGGGA ATGATTTTGT TTCTATTGTT TACAATGATT CTGGTGAGGA CTTCAAACTG
    GGAACAATAA AGGGTCAGTT CAATTTTGTG CATGTTATCA TAACCCCACT TGACTATGAC
    TGCAACCTGG TCACACTGCA GTGTCGAAAA GACATGGAGG GCCTTGTAGA CACGAGTGTT
    GCTAAGATCA TCTCTGACAA GAACCTGCCA TTCGTGGCCC GTCAGATGGC CCTGCATGCT
    AACATGGCTT CCCAGGTGCA CCACAGTCGA TCCAACCCTA CTGACACCTA CCCATCCAAA
    TGGATCGCTC GGCTGCGGCA CATCAAACGG CTGAGGCACC GGCTACGTGA AGAAACCCAG
    TACCAAACTC CTGGCCTTCC CCTGCAGATG CACCCGTCTG CTCCAACAAA GCCCTCTCCC
    CAGATGGCTC AGGACCCACC GCCGGCCTAC GAGACCGGTC AGAGGAAGCG GCTGATCTCC
    TCGGTGGATG ACTTCACTGA GTTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149936.1
    CDS58..5487
    Translation

    Target ORF information:

    RefSeq Version XM_015294450.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294450.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TTTAAGACTA GCCAAAAATG CAAAGCAAGG CTTGAATAAC
    TCTCCTCCAG TGAAAGAGCT GAAAGAACCC TCTGCAGTTG ATGCCTTCCG ATCCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGT CGGATACAGA CATCCATCAC TAGTTCCAGC
    TTGGGCTCTG CAGATGAAAA TTCAATGGCT CAGGCTGATG ACAACTTAAA AAATCTCCAT
    CTGGAACTCA CTGAGACTTG TCTGGATATG ATGGCCCGAT ACGTCTTTTC CAACTTCACT
    GCGGTGCCTA AGAGGTCTCC TGTGGGTGAG TTTCTGTTGG CTGGAGGAAG GACAAAGACA
    TGGCTGGTTG GCAACAAGTT GGTGACCATC ACTACAAGTG TCGGAACAGG GACAAGGTCC
    TTGCTTGGCC TGGACTCTGG AGATCTCCAA AGCAGCACAG AATCAGGCTC AGACCCGGTT
    TTGCAAGTGA GACAAACTAA AGAAGCTCCA GCAAAGCTCG AATCTCAAGC TGGGCAGCAG
    GTTTGCAGAA GCTCTCGCAA CAGAGTCAGA TCCATGTCTG GTGGTCATGC CTTACGTGTT
    GGTGCCTTAG ACAGCACAGC CTCCCACTTC CCTGGTGGCT CGGCTTCACA AGGGACACAG
    AGTCCTCCTG CTCCTGCACC TCGGTCAGAG AAGACTAATC CTGCTCCACA GACACCTCTG
    CAGAAAGAAA AAGCGAACTT AGCTGCATAT GTCCCACTGC TGACACAGGG CTGGGCAGAA
    ATCCTTGTGC GCAGACCCAC AGGTAACACA AGCTGGCTAA TGAGTCTGGA GAACCCGCTC
    AGTCCCTTTT CTTCAGACAT TAACAATATG CCCCTGCAAG AGCTGTCCAA CGCACTCATG
    GCTGCAGAGC GTTTTAAAGA GCACCGAGAA ACAGCTCTCT ACAAATCCCT GTCTGTCCCT
    TCCTCCAGCT TGGCCACTGG GACAGCCAAG CCTTCACTTC TCCAGCGTTC CAACACAGAG
    TCTGCGGTCG TGCTGGAGGA AGGGAGCCCC TTGGAAATGG AGCTGAAGGA AACTGAGTCC
    CCAGCTGAGT CTCCAGAGAG TGAAGATTTT GAGACGTCGT GTTCTGAGCA AGTCCTGAGC
    GAGGAAAGGT TTGGTAAAGC CCAGGAGTTT TACAGCAGGT CGTCCTCGAC CTCTAGCCAA
    GATGAAAAAT CTCTGAAGTC GGACGAGCTG GTGGAGGGCG GTATTCCCAT CGGGAGAGTG
    CTGCCAGCAG AGGATGGCCG GGCACTGGAG GAGCTCTCCT TCCAGCCCTC CCAGCCGCTC
    AGCAAATCGA GCTCTTCCCC CGAGCTGCAG ACTTTGCAGG AGATCCTCAA GGATGCAAAT
    GGCAGAGAAG TAACAAGGAG GCTGAGCACG GAAGTGAAGT CCAAATCTCA GTCTGGGAAC
    CTGGAAGGGG AAGGACTGGG CAGTTGGCTG AACAAGGGTG AGGATGCCAG AGCAGCAGGC
    TCGGGGGGGT TGGATGGCAG TGGCCCCACC ACCTCGCCAC GCTCCCCATC TGGGCACCGG
    CCCCGGGGGT ACACCATCTC TGACTCTGCC CCATCACGGA GAGGGAAGAG GATGGAGAGA
    GATGCCTTCA AGAGCAGAAC AGCAGCCACC AATGCTGAGA AAGTACCTGG AATAAACCCA
    AGCTTTGTGT TTTTACAATT GTACCATTCC CCGTTCTTTG GTGATGAAAA CAACAAGCCC
    CTCCTGTTGC CAAATGAGAC GTTCGAAAAA TCGGTGCAGC TCCTGGATCA AATTCCTTCG
    TATGACACCC ACAAGATCGC AGTGCTGTAC GTTGGAGAGG GCCAGAGCAA CAATGAAATC
    GCTATTCTGT CCAATGAACA CGGCTCCTAT CGCTACACCG AGTTCCTGAC AGGCTTAGGG
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCA GATAAAATCT ACCTCGGTGG CCTGGACGTG
    TGTGGGGAGG ATGGACAGTT TACCTACTGC TGGCACGATG ACATCATGCA AGCTATTTTC
    CACATTGCTA CACTGATGCC CACAAAGGAT TTGGATAAAT ATCGCTGTGA CAAGAAGAGG
    CACCTTGGGA ATGATTTTGT TTCTATTGTT TACAATGATT CTGGTGAGGA CTTCAAACTG
    GGAACAATAA AGGGTCAGTT CAATTTTGTG CATGTTATCA TAACCCCACT TGACTATGAC
    TGCAACCTGG TCACACTGCA GTGTCGAAAA GACATGGAGG GCCTTGTAGA CACGAGTGTT
    GCTAAGATCA TCTCTGACAA GAACCTGCCA TTCGTGGCCC GTCAGATGGC CCTGCATGCT
    AACATGGCTT CCCAGGTGCA CCACAGTCGA TCCAACCCTA CTGACACCTA CCCATCCAAA
    TGGATCGCTC GGCTGCGGCA CATCAAACGG CTGAGGCACC GGCTACGTGA AGAAACCCAG
    TACCAAACTC CTGGCCTTCC CCTGCAGATG CACCCGTCTG CTCCAACAAA GCCCTCTCCC
    CAGATGGCTC AGGACCCACC GCCGGCCTAC GAGACCGGTC AGAGGAAGCG GCTGATCTCC
    TCGGTGGATG ACTTCACTGA GTTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42376
    Clone ID Related Accession (Same CDS sequence) XM_015294450.2 , XM_015294450.1
    Accession Version XM_015294450.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tuberin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TTTAAGACTA GCCAAAAATG CAAAGCAAGG CTTGAATAAC
    TCTCCTCCAG TGAAAGAGCT GAAAGAACCC TCTGCAGTTG ATGCCTTCCG ATCCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGT CGGATACAGA CATCCATCAC TAGTTCCAGC
    TTGGGCTCTG CAGATGAAAA TTCAATGGCT CAGGCTGATG ACAACTTAAA AAATCTCCAT
    CTGGAACTCA CTGAGACTTG TCTGGATATG ATGGCCCGAT ACGTCTTTTC CAACTTCACT
    GCGGTGCCTA AGAGGTCTCC TGTGGGTGAG TTTCTGTTGG CTGGAGGAAG GACAAAGACA
    TGGCTGGTTG GCAACAAGTT GGTGACCATC ACTACAAGTG TCGGAACAGG GACAAGGTCC
    TTGCTTGGCC TGGACTCTGG AGATCTCCAA AGCAGCACAG AATCAGGCTC AGACCCGGTT
    TTGCAAGTGA GACAAACTAA AGAAGCTCCA GCAAAGCTCG AATCTCAAGC TGGGCAGCAG
    GTTTGCAGAA GCTCTCGCAA CAGAGTCAGA TCCATGTCTG GTGGTCATGC CTTACGTGTT
    GGTGCCTTAG ACAGCACAGC CTCCCACTTC CCTGGTGGCT CGGCTTCACA AGGGACACAG
    AGTCCTCCTG CTCCTGCACC TCGGTCAGAG AAGACTAATC CTGCTCCACA GACACCTCTG
    CAGAAAGAAA AAGCGAACTT AGCTGCATAT GTCCCACTGC TGACACAGGG CTGGGCAGAA
    ATCCTTGTGC GCAGACCCAC AGGTAACACA AGCTGGCTAA TGAGTCTGGA GAACCCGCTC
    AGTCCCTTTT CTTCAGACAT TAACAATATG CCCCTGCAAG AGCTGTCCAA CGCACTCATG
    GCTGCAGAGC GTTTTAAAGA GCACCGAGAA ACAGCTCTCT ACAAATCCCT GTCTGTCCCT
    TCCTCCAGCT TGGCCACTGG GACAGCCAAG CCTTCACTTC TCCAGCGTTC CAACACAGAG
    TCTGCGGTCG TGCTGGAGGA AGGGAGCCCC TTGGAAATGG AGCTGAAGGA AACTGAGTCC
    CCAGCTGAGT CTCCAGAGAG TGAAGATTTT GAGACGTCGT GTTCTGAGCA AGTCCTGAGC
    GAGGAAAGGT TTGGTAAAGC CCAGGAGTTT TACAGCAGGT CGTCCTCGAC CTCTAGCCAA
    GATGAAAAAT CTCTGAAGTC GGACGAGCTG GTGGAGGGCG GTATTCCCAT CGGGAGAGTG
    CTGCCAGCAG AGGATGGCCG GGCACTGGAG GAGCTCTCCT TCCAGCCCTC CCAGCCGCTC
    AGCAAATCGA GCTCTTCCCC CGAGCTGCAG ACTTTGCAGG AGATCCTCAA GGATGCAAAT
    GGCAGAGAAG TAACAAGGAG GCTGAGCACG GAAGTGAAGT CCAAATCTCA GTCTGGGAAC
    CTGGAAGGGG AAGGACTGGG CAGTTGGCTG AACAAGGGTG AGGATGCCAG AGCAGCAGGC
    TCGGGGGGGT TGGATGGCAG TGGCCCCACC ACCTCGCCAC GCTCCCCATC TGGGCACCGG
    CCCCGGGGGT ACACCATCTC TGACTCTGCC CCATCACGGA GAGGGAAGAG GATGGAGAGA
    GATGCCTTCA AGAGCAGAAC AGCAGCCACC AATGCTGAGA AAGTACCTGG AATAAACCCA
    AGCTTTGTGT TTTTACAATT GTACCATTCC CCGTTCTTTG GTGATGAAAA CAACAAGCCC
    CTCCTGTTGC CAAATGAGAC GTTCGAAAAA TCGGTGCAGC TCCTGGATCA AATTCCTTCG
    TATGACACCC ACAAGATCGC AGTGCTGTAC GTTGGAGAGG GCCAGAGCAA CAATGAAATC
    GCTATTCTGT CCAATGAACA CGGCTCCTAT CGCTACACCG AGTTCCTGAC AGGCTTAGGG
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCA GATAAAATCT ACCTCGGTGG CCTGGACGTG
    TGTGGGGAGG ATGGACAGTT TACCTACTGC TGGCACGATG ACATCATGCA AGCTATTTTC
    CACATTGCTA CACTGATGCC CACAAAGGAT TTGGATAAAT ATCGCTGTGA CAAGAAGAGG
    CACCTTGGGA ATGATTTTGT TTCTATTGTT TACAATGATT CTGGTGAGGA CTTCAAACTG
    GGAACAATAA AGGGTCAGTT CAATTTTGTG CATGTTATCA TAACCCCACT TGACTATGAC
    TGCAACCTGG TCACACTGCA GTGTCGAAAA GACATGGAGG GCCTTGTAGA CACGAGTGTT
    GCTAAGATCA TCTCTGACAA GAACCTGCCA TTCGTGGCCC GTCAGATGGC CCTGCATGCT
    AACATGGCTT CCCAGGTGCA CCACAGTCGA TCCAACCCTA CTGACACCTA CCCATCCAAA
    TGGATCGCTC GGCTGCGGCA CATCAAACGG CTGAGGCACC GGCTACGTGA AGAAACCCAG
    TACCAAACTC CTGGCCTTCC CCTGCAGATG CACCCGTCTG CTCCAACAAA GCCCTCTCCC
    CAGATGGCTC AGGACCCACC GCCGGCCTAC GAGACCGGTC AGAGGAAGCG GCTGATCTCC
    TCGGTGGATG ACTTCACTGA GTTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149936.1
    CDS58..5487
    Translation

    Target ORF information:

    RefSeq Version XM_015294450.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294450.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TTTAAGACTA GCCAAAAATG CAAAGCAAGG CTTGAATAAC
    TCTCCTCCAG TGAAAGAGCT GAAAGAACCC TCTGCAGTTG ATGCCTTCCG ATCCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGT CGGATACAGA CATCCATCAC TAGTTCCAGC
    TTGGGCTCTG CAGATGAAAA TTCAATGGCT CAGGCTGATG ACAACTTAAA AAATCTCCAT
    CTGGAACTCA CTGAGACTTG TCTGGATATG ATGGCCCGAT ACGTCTTTTC CAACTTCACT
    GCGGTGCCTA AGAGGTCTCC TGTGGGTGAG TTTCTGTTGG CTGGAGGAAG GACAAAGACA
    TGGCTGGTTG GCAACAAGTT GGTGACCATC ACTACAAGTG TCGGAACAGG GACAAGGTCC
    TTGCTTGGCC TGGACTCTGG AGATCTCCAA AGCAGCACAG AATCAGGCTC AGACCCGGTT
    TTGCAAGTGA GACAAACTAA AGAAGCTCCA GCAAAGCTCG AATCTCAAGC TGGGCAGCAG
    GTTTGCAGAA GCTCTCGCAA CAGAGTCAGA TCCATGTCTG GTGGTCATGC CTTACGTGTT
    GGTGCCTTAG ACAGCACAGC CTCCCACTTC CCTGGTGGCT CGGCTTCACA AGGGACACAG
    AGTCCTCCTG CTCCTGCACC TCGGTCAGAG AAGACTAATC CTGCTCCACA GACACCTCTG
    CAGAAAGAAA AAGCGAACTT AGCTGCATAT GTCCCACTGC TGACACAGGG CTGGGCAGAA
    ATCCTTGTGC GCAGACCCAC AGGTAACACA AGCTGGCTAA TGAGTCTGGA GAACCCGCTC
    AGTCCCTTTT CTTCAGACAT TAACAATATG CCCCTGCAAG AGCTGTCCAA CGCACTCATG
    GCTGCAGAGC GTTTTAAAGA GCACCGAGAA ACAGCTCTCT ACAAATCCCT GTCTGTCCCT
    TCCTCCAGCT TGGCCACTGG GACAGCCAAG CCTTCACTTC TCCAGCGTTC CAACACAGAG
    TCTGCGGTCG TGCTGGAGGA AGGGAGCCCC TTGGAAATGG AGCTGAAGGA AACTGAGTCC
    CCAGCTGAGT CTCCAGAGAG TGAAGATTTT GAGACGTCGT GTTCTGAGCA AGTCCTGAGC
    GAGGAAAGGT TTGGTAAAGC CCAGGAGTTT TACAGCAGGT CGTCCTCGAC CTCTAGCCAA
    GATGAAAAAT CTCTGAAGTC GGACGAGCTG GTGGAGGGCG GTATTCCCAT CGGGAGAGTG
    CTGCCAGCAG AGGATGGCCG GGCACTGGAG GAGCTCTCCT TCCAGCCCTC CCAGCCGCTC
    AGCAAATCGA GCTCTTCCCC CGAGCTGCAG ACTTTGCAGG AGATCCTCAA GGATGCAAAT
    GGCAGAGAAG TAACAAGGAG GCTGAGCACG GAAGTGAAGT CCAAATCTCA GTCTGGGAAC
    CTGGAAGGGG AAGGACTGGG CAGTTGGCTG AACAAGGGTG AGGATGCCAG AGCAGCAGGC
    TCGGGGGGGT TGGATGGCAG TGGCCCCACC ACCTCGCCAC GCTCCCCATC TGGGCACCGG
    CCCCGGGGGT ACACCATCTC TGACTCTGCC CCATCACGGA GAGGGAAGAG GATGGAGAGA
    GATGCCTTCA AGAGCAGAAC AGCAGCCACC AATGCTGAGA AAGTACCTGG AATAAACCCA
    AGCTTTGTGT TTTTACAATT GTACCATTCC CCGTTCTTTG GTGATGAAAA CAACAAGCCC
    CTCCTGTTGC CAAATGAGAC GTTCGAAAAA TCGGTGCAGC TCCTGGATCA AATTCCTTCG
    TATGACACCC ACAAGATCGC AGTGCTGTAC GTTGGAGAGG GCCAGAGCAA CAATGAAATC
    GCTATTCTGT CCAATGAACA CGGCTCCTAT CGCTACACCG AGTTCCTGAC AGGCTTAGGG
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCA GATAAAATCT ACCTCGGTGG CCTGGACGTG
    TGTGGGGAGG ATGGACAGTT TACCTACTGC TGGCACGATG ACATCATGCA AGCTATTTTC
    CACATTGCTA CACTGATGCC CACAAAGGAT TTGGATAAAT ATCGCTGTGA CAAGAAGAGG
    CACCTTGGGA ATGATTTTGT TTCTATTGTT TACAATGATT CTGGTGAGGA CTTCAAACTG
    GGAACAATAA AGGGTCAGTT CAATTTTGTG CATGTTATCA TAACCCCACT TGACTATGAC
    TGCAACCTGG TCACACTGCA GTGTCGAAAA GACATGGAGG GCCTTGTAGA CACGAGTGTT
    GCTAAGATCA TCTCTGACAA GAACCTGCCA TTCGTGGCCC GTCAGATGGC CCTGCATGCT
    AACATGGCTT CCCAGGTGCA CCACAGTCGA TCCAACCCTA CTGACACCTA CCCATCCAAA
    TGGATCGCTC GGCTGCGGCA CATCAAACGG CTGAGGCACC GGCTACGTGA AGAAACCCAG
    TACCAAACTC CTGGCCTTCC CCTGCAGATG CACCCGTCTG CTCCAACAAA GCCCTCTCCC
    CAGATGGCTC AGGACCCACC GCCGGCCTAC GAGACCGGTC AGAGGAAGCG GCTGATCTCC
    TCGGTGGATG ACTTCACTGA GTTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42377
    Clone ID Related Accession (Same CDS sequence) XM_015294451.2 , XM_015294451.1
    Accession Version XM_015294451.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tuberin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TCGGATACAG ACATCCATCA CTAGTTCCAG CTTGGGCTCT
    GCAGATGAAA ATTCAATGGC TCAGGCTGAT GACAACTTAA AAAATCTCCA TCTGGAACTC
    ACTGAGACTT GTCTGGATAT GATGGCCCGA TACGTCTTTT CCAACTTCAC TGCGGTGCCT
    AAGAGGTCTC CTGTGGGTGA GTTTCTGTTG GCTGGAGGAA GGACAAAGAC ATGGCTGGTT
    GGCAACAAGT TGGTGACCAT CACTACAAGT GTCGGAACAG GGACAAGGTC CTTGCTTGGC
    CTGGACTCTG GAGATCTCCA AAGCAGCACA GAATCAGGCT CAGACCCGGT TTTGCAAGTG
    AGACAAACTA AAGAAGCTCC AGCAAAGCTC GAATCTCAAG CTGGGCAGCA GGTTTGCAGA
    AGCTCTCGCA ACAGAGTCAG ATCCATGTCT GGTGGTCATG CCTTACGTGT TGGTGCCTTA
    GACAGCACAG CCTCCCACTT CCCTGGTGGC TCGGCTTCAC AAGGGACACA GAGTCCTCCT
    GCTCCTGCAC CTCGGTCAGA GAAGACTAAT CCTGCTCCAC AGACACCTCT GCAGAAAGAA
    AAAGCGAACT TAGCTGCATA TGTCCCACTG CTGACACAGG GCTGGGCAGA AATCCTTGTG
    CGCAGACCCA CAGGTAACAC AAGCTGGCTA ATGAGTCTGG AGAACCCGCT CAGTCCCTTT
    TCTTCAGACA TTAACAATAT GCCCCTGCAA GAGCTGTCCA ACGCACTCAT GGCTGCAGAG
    CGTTTTAAAG AGCACCGAGA AACAGCTCTC TACAAATCCC TGTCTGTCCC TTCCTCCAGC
    TTGGCCACTG GGACAGCCAA GCCTTCACTT CTCCAGCGTT CCAACACAGT GGCCTCTTTC
    TCTTCCATGT ACCAGTCCAG TTGTCAAGGG AAGTTGCACA GGAGCATATC CTGGGCAGAG
    TCTGCGGTCG TGCTGGAGGA AGGGAGCCCC TTGGAAATGG AGCTGAAGGA AACTGAGTCC
    CCAGCTGAGT CTCCAGAGAG TGAAGATTTT GAGACGTCGT GTTCTGAGCA AGTCCTGAGC
    GAGGAAAGGT TTGGTAAAGC CCAGGAGTTT TACAGCAGGT CGTCCTCGAC CTCTAGCCAA
    GATGAAAAAT CTCTGAAGTC GGACGAGCTG GTGGAGGGCG GTATTCCCAT CGGGAGAGTG
    CTGCCAGCAG AGGATGGCCG GGCACTGGAG GAGCTCTCCT TCCAGCCCTC CCAGCCGCTC
    AGCAAATCGA GCTCTTCCCC CGAGCTGCAG ACTTTGCAGG AGATCCTCAA GGATGCAAAT
    GGCAGAGAAG TAACAAGGAG GCTGAGCACG GAAGTGAAGT CCAAATCTCA GTCTGGGAAC
    CTGGAAGGGG AAGGACTGGG CAGTTGGCTG AACAAGGGTG AGGATGCCAG AGCAGCAGGC
    TCGGGGGGGT TGGATGGCAG TGGCCCCACC ACCTCGCCAC GCTCCCCATC TGGGCACCGG
    CCCCGGGGGT ACACCATCTC TGACTCTGCC CCATCACGGA GAGGGAAGAG GATGGAGAGA
    GATGCCTTCA AGAGCAGAAC AGCAGCCACC AATGCTGAGA AAGTACCTGG AATAAACCCA
    AGCTTTGTGT TTTTACAATT GTACCATTCC CCGTTCTTTG GTGATGAAAA CAACAAGCCC
    CTCCTGTTGC CAAATGAGAC GTTCGAAAAA TCGGTGCAGC TCCTGGATCA AATTCCTTCG
    TATGACACCC ACAAGATCGC AGTGCTGTAC GTTGGAGAGG GCCAGAGCAA CAATGAAATC
    GCTATTCTGT CCAATGAACA CGGCTCCTAT CGCTACACCG AGTTCCTGAC AGGCTTAGGG
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCA GATAAAATCT ACCTCGGTGG CCTGGACGTG
    TGTGGGGAGG ATGGACAGTT TACCTACTGC TGGCACGATG ACATCATGCA AGCTATTTTC
    CACATTGCTA CACTGATGCC CACAAAGGAT TTGGATAAAT ATCGCTGTGA CAAGAAGAGG
    CACCTTGGGA ATGATTTTGT TTCTATTGTT TACAATGATT CTGGTGAGGA CTTCAAACTG
    GGAACAATAA AGGGTCAGTT CAATTTTGTG CATGTTATCA TAACCCCACT TGACTATGAC
    TGCAACCTGG TCACACTGCA GTGTCGAAAA GACATGGAGG GCCTTGTAGA CACGAGTGTT
    GCTAAGATCA TCTCTGACAA GAACCTGCCA TTCGTGGCCC GTCAGATGGC CCTGCATGCT
    AACATGGCTT CCCAGGTGCA CCACAGTCGA TCCAACCCTA CTGACACCTA CCCATCCAAA
    TGGATCGCTC GGCTGCGGCA CATCAAACGG CTGAGGCACC GGCTACGTGA AGAAACCCAG
    TACCAAACTC CTGGCCTTCC CCTGCAGATG CACCCGTCTG CTCCAACAAA GCCCTCTCCC
    CAGATGGCTC AGGACCCACC GCCGGCCTAC GAGACCGGTC AGAGGAAGCG GCTGATCTCC
    TCGGTGGATG ACTTCACTGA GTTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149937.1
    CDS58..5427
    Translation

    Target ORF information:

    RefSeq Version XM_015294451.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294451.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TCGGATACAG ACATCCATCA CTAGTTCCAG CTTGGGCTCT
    GCAGATGAAA ATTCAATGGC TCAGGCTGAT GACAACTTAA AAAATCTCCA TCTGGAACTC
    ACTGAGACTT GTCTGGATAT GATGGCCCGA TACGTCTTTT CCAACTTCAC TGCGGTGCCT
    AAGAGGTCTC CTGTGGGTGA GTTTCTGTTG GCTGGAGGAA GGACAAAGAC ATGGCTGGTT
    GGCAACAAGT TGGTGACCAT CACTACAAGT GTCGGAACAG GGACAAGGTC CTTGCTTGGC
    CTGGACTCTG GAGATCTCCA AAGCAGCACA GAATCAGGCT CAGACCCGGT TTTGCAAGTG
    AGACAAACTA AAGAAGCTCC AGCAAAGCTC GAATCTCAAG CTGGGCAGCA GGTTTGCAGA
    AGCTCTCGCA ACAGAGTCAG ATCCATGTCT GGTGGTCATG CCTTACGTGT TGGTGCCTTA
    GACAGCACAG CCTCCCACTT CCCTGGTGGC TCGGCTTCAC AAGGGACACA GAGTCCTCCT
    GCTCCTGCAC CTCGGTCAGA GAAGACTAAT CCTGCTCCAC AGACACCTCT GCAGAAAGAA
    AAAGCGAACT TAGCTGCATA TGTCCCACTG CTGACACAGG GCTGGGCAGA AATCCTTGTG
    CGCAGACCCA CAGGTAACAC AAGCTGGCTA ATGAGTCTGG AGAACCCGCT CAGTCCCTTT
    TCTTCAGACA TTAACAATAT GCCCCTGCAA GAGCTGTCCA ACGCACTCAT GGCTGCAGAG
    CGTTTTAAAG AGCACCGAGA AACAGCTCTC TACAAATCCC TGTCTGTCCC TTCCTCCAGC
    TTGGCCACTG GGACAGCCAA GCCTTCACTT CTCCAGCGTT CCAACACAGT GGCCTCTTTC
    TCTTCCATGT ACCAGTCCAG TTGTCAAGGG AAGTTGCACA GGAGCATATC CTGGGCAGAG
    TCTGCGGTCG TGCTGGAGGA AGGGAGCCCC TTGGAAATGG AGCTGAAGGA AACTGAGTCC
    CCAGCTGAGT CTCCAGAGAG TGAAGATTTT GAGACGTCGT GTTCTGAGCA AGTCCTGAGC
    GAGGAAAGGT TTGGTAAAGC CCAGGAGTTT TACAGCAGGT CGTCCTCGAC CTCTAGCCAA
    GATGAAAAAT CTCTGAAGTC GGACGAGCTG GTGGAGGGCG GTATTCCCAT CGGGAGAGTG
    CTGCCAGCAG AGGATGGCCG GGCACTGGAG GAGCTCTCCT TCCAGCCCTC CCAGCCGCTC
    AGCAAATCGA GCTCTTCCCC CGAGCTGCAG ACTTTGCAGG AGATCCTCAA GGATGCAAAT
    GGCAGAGAAG TAACAAGGAG GCTGAGCACG GAAGTGAAGT CCAAATCTCA GTCTGGGAAC
    CTGGAAGGGG AAGGACTGGG CAGTTGGCTG AACAAGGGTG AGGATGCCAG AGCAGCAGGC
    TCGGGGGGGT TGGATGGCAG TGGCCCCACC ACCTCGCCAC GCTCCCCATC TGGGCACCGG
    CCCCGGGGGT ACACCATCTC TGACTCTGCC CCATCACGGA GAGGGAAGAG GATGGAGAGA
    GATGCCTTCA AGAGCAGAAC AGCAGCCACC AATGCTGAGA AAGTACCTGG AATAAACCCA
    AGCTTTGTGT TTTTACAATT GTACCATTCC CCGTTCTTTG GTGATGAAAA CAACAAGCCC
    CTCCTGTTGC CAAATGAGAC GTTCGAAAAA TCGGTGCAGC TCCTGGATCA AATTCCTTCG
    TATGACACCC ACAAGATCGC AGTGCTGTAC GTTGGAGAGG GCCAGAGCAA CAATGAAATC
    GCTATTCTGT CCAATGAACA CGGCTCCTAT CGCTACACCG AGTTCCTGAC AGGCTTAGGG
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCA GATAAAATCT ACCTCGGTGG CCTGGACGTG
    TGTGGGGAGG ATGGACAGTT TACCTACTGC TGGCACGATG ACATCATGCA AGCTATTTTC
    CACATTGCTA CACTGATGCC CACAAAGGAT TTGGATAAAT ATCGCTGTGA CAAGAAGAGG
    CACCTTGGGA ATGATTTTGT TTCTATTGTT TACAATGATT CTGGTGAGGA CTTCAAACTG
    GGAACAATAA AGGGTCAGTT CAATTTTGTG CATGTTATCA TAACCCCACT TGACTATGAC
    TGCAACCTGG TCACACTGCA GTGTCGAAAA GACATGGAGG GCCTTGTAGA CACGAGTGTT
    GCTAAGATCA TCTCTGACAA GAACCTGCCA TTCGTGGCCC GTCAGATGGC CCTGCATGCT
    AACATGGCTT CCCAGGTGCA CCACAGTCGA TCCAACCCTA CTGACACCTA CCCATCCAAA
    TGGATCGCTC GGCTGCGGCA CATCAAACGG CTGAGGCACC GGCTACGTGA AGAAACCCAG
    TACCAAACTC CTGGCCTTCC CCTGCAGATG CACCCGTCTG CTCCAACAAA GCCCTCTCCC
    CAGATGGCTC AGGACCCACC GCCGGCCTAC GAGACCGGTC AGAGGAAGCG GCTGATCTCC
    TCGGTGGATG ACTTCACTGA GTTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42377
    Clone ID Related Accession (Same CDS sequence) XM_015294451.2 , XM_015294451.1
    Accession Version XM_015294451.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tuberin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TCGGATACAG ACATCCATCA CTAGTTCCAG CTTGGGCTCT
    GCAGATGAAA ATTCAATGGC TCAGGCTGAT GACAACTTAA AAAATCTCCA TCTGGAACTC
    ACTGAGACTT GTCTGGATAT GATGGCCCGA TACGTCTTTT CCAACTTCAC TGCGGTGCCT
    AAGAGGTCTC CTGTGGGTGA GTTTCTGTTG GCTGGAGGAA GGACAAAGAC ATGGCTGGTT
    GGCAACAAGT TGGTGACCAT CACTACAAGT GTCGGAACAG GGACAAGGTC CTTGCTTGGC
    CTGGACTCTG GAGATCTCCA AAGCAGCACA GAATCAGGCT CAGACCCGGT TTTGCAAGTG
    AGACAAACTA AAGAAGCTCC AGCAAAGCTC GAATCTCAAG CTGGGCAGCA GGTTTGCAGA
    AGCTCTCGCA ACAGAGTCAG ATCCATGTCT GGTGGTCATG CCTTACGTGT TGGTGCCTTA
    GACAGCACAG CCTCCCACTT CCCTGGTGGC TCGGCTTCAC AAGGGACACA GAGTCCTCCT
    GCTCCTGCAC CTCGGTCAGA GAAGACTAAT CCTGCTCCAC AGACACCTCT GCAGAAAGAA
    AAAGCGAACT TAGCTGCATA TGTCCCACTG CTGACACAGG GCTGGGCAGA AATCCTTGTG
    CGCAGACCCA CAGGTAACAC AAGCTGGCTA ATGAGTCTGG AGAACCCGCT CAGTCCCTTT
    TCTTCAGACA TTAACAATAT GCCCCTGCAA GAGCTGTCCA ACGCACTCAT GGCTGCAGAG
    CGTTTTAAAG AGCACCGAGA AACAGCTCTC TACAAATCCC TGTCTGTCCC TTCCTCCAGC
    TTGGCCACTG GGACAGCCAA GCCTTCACTT CTCCAGCGTT CCAACACAGT GGCCTCTTTC
    TCTTCCATGT ACCAGTCCAG TTGTCAAGGG AAGTTGCACA GGAGCATATC CTGGGCAGAG
    TCTGCGGTCG TGCTGGAGGA AGGGAGCCCC TTGGAAATGG AGCTGAAGGA AACTGAGTCC
    CCAGCTGAGT CTCCAGAGAG TGAAGATTTT GAGACGTCGT GTTCTGAGCA AGTCCTGAGC
    GAGGAAAGGT TTGGTAAAGC CCAGGAGTTT TACAGCAGGT CGTCCTCGAC CTCTAGCCAA
    GATGAAAAAT CTCTGAAGTC GGACGAGCTG GTGGAGGGCG GTATTCCCAT CGGGAGAGTG
    CTGCCAGCAG AGGATGGCCG GGCACTGGAG GAGCTCTCCT TCCAGCCCTC CCAGCCGCTC
    AGCAAATCGA GCTCTTCCCC CGAGCTGCAG ACTTTGCAGG AGATCCTCAA GGATGCAAAT
    GGCAGAGAAG TAACAAGGAG GCTGAGCACG GAAGTGAAGT CCAAATCTCA GTCTGGGAAC
    CTGGAAGGGG AAGGACTGGG CAGTTGGCTG AACAAGGGTG AGGATGCCAG AGCAGCAGGC
    TCGGGGGGGT TGGATGGCAG TGGCCCCACC ACCTCGCCAC GCTCCCCATC TGGGCACCGG
    CCCCGGGGGT ACACCATCTC TGACTCTGCC CCATCACGGA GAGGGAAGAG GATGGAGAGA
    GATGCCTTCA AGAGCAGAAC AGCAGCCACC AATGCTGAGA AAGTACCTGG AATAAACCCA
    AGCTTTGTGT TTTTACAATT GTACCATTCC CCGTTCTTTG GTGATGAAAA CAACAAGCCC
    CTCCTGTTGC CAAATGAGAC GTTCGAAAAA TCGGTGCAGC TCCTGGATCA AATTCCTTCG
    TATGACACCC ACAAGATCGC AGTGCTGTAC GTTGGAGAGG GCCAGAGCAA CAATGAAATC
    GCTATTCTGT CCAATGAACA CGGCTCCTAT CGCTACACCG AGTTCCTGAC AGGCTTAGGG
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCA GATAAAATCT ACCTCGGTGG CCTGGACGTG
    TGTGGGGAGG ATGGACAGTT TACCTACTGC TGGCACGATG ACATCATGCA AGCTATTTTC
    CACATTGCTA CACTGATGCC CACAAAGGAT TTGGATAAAT ATCGCTGTGA CAAGAAGAGG
    CACCTTGGGA ATGATTTTGT TTCTATTGTT TACAATGATT CTGGTGAGGA CTTCAAACTG
    GGAACAATAA AGGGTCAGTT CAATTTTGTG CATGTTATCA TAACCCCACT TGACTATGAC
    TGCAACCTGG TCACACTGCA GTGTCGAAAA GACATGGAGG GCCTTGTAGA CACGAGTGTT
    GCTAAGATCA TCTCTGACAA GAACCTGCCA TTCGTGGCCC GTCAGATGGC CCTGCATGCT
    AACATGGCTT CCCAGGTGCA CCACAGTCGA TCCAACCCTA CTGACACCTA CCCATCCAAA
    TGGATCGCTC GGCTGCGGCA CATCAAACGG CTGAGGCACC GGCTACGTGA AGAAACCCAG
    TACCAAACTC CTGGCCTTCC CCTGCAGATG CACCCGTCTG CTCCAACAAA GCCCTCTCCC
    CAGATGGCTC AGGACCCACC GCCGGCCTAC GAGACCGGTC AGAGGAAGCG GCTGATCTCC
    TCGGTGGATG ACTTCACTGA GTTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149937.1
    CDS58..5427
    Translation

    Target ORF information:

    RefSeq Version XM_015294451.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TCGGATACAG ACATCCATCA CTAGTTCCAG CTTGGGCTCT
    GCAGATGAAA ATTCAATGGC TCAGGCTGAT GACAACTTAA AAAATCTCCA TCTGGAACTC
    ACTGAGACTT GTCTGGATAT GATGGCCCGA TACGTCTTTT CCAACTTCAC TGCGGTGCCT
    AAGAGGTCTC CTGTGGGTGA GTTTCTGTTG GCTGGAGGAA GGACAAAGAC ATGGCTGGTT
    GGCAACAAGT TGGTGACCAT CACTACAAGT GTCGGAACAG GGACAAGGTC CTTGCTTGGC
    CTGGACTCTG GAGATCTCCA AAGCAGCACA GAATCAGGCT CAGACCCGGT TTTGCAAGTG
    AGACAAACTA AAGAAGCTCC AGCAAAGCTC GAATCTCAAG CTGGGCAGCA GGTTTGCAGA
    AGCTCTCGCA ACAGAGTCAG ATCCATGTCT GGTGGTCATG CCTTACGTGT TGGTGCCTTA
    GACAGCACAG CCTCCCACTT CCCTGGTGGC TCGGCTTCAC AAGGGACACA GAGTCCTCCT
    GCTCCTGCAC CTCGGTCAGA GAAGACTAAT CCTGCTCCAC AGACACCTCT GCAGAAAGAA
    AAAGCGAACT TAGCTGCATA TGTCCCACTG CTGACACAGG GCTGGGCAGA AATCCTTGTG
    CGCAGACCCA CAGGTAACAC AAGCTGGCTA ATGAGTCTGG AGAACCCGCT CAGTCCCTTT
    TCTTCAGACA TTAACAATAT GCCCCTGCAA GAGCTGTCCA ACGCACTCAT GGCTGCAGAG
    CGTTTTAAAG AGCACCGAGA AACAGCTCTC TACAAATCCC TGTCTGTCCC TTCCTCCAGC
    TTGGCCACTG GGACAGCCAA GCCTTCACTT CTCCAGCGTT CCAACACAGT GGCCTCTTTC
    TCTTCCATGT ACCAGTCCAG TTGTCAAGGG AAGTTGCACA GGAGCATATC CTGGGCAGAG
    TCTGCGGTCG TGCTGGAGGA AGGGAGCCCC TTGGAAATGG AGCTGAAGGA AACTGAGTCC
    CCAGCTGAGT CTCCAGAGAG TGAAGATTTT GAGACGTCGT GTTCTGAGCA AGTCCTGAGC
    GAGGAAAGGT TTGGTAAAGC CCAGGAGTTT TACAGCAGGT CGTCCTCGAC CTCTAGCCAA
    GATGAAAAAT CTCTGAAGTC GGACGAGCTG GTGGAGGGCG GTATTCCCAT CGGGAGAGTG
    CTGCCAGCAG AGGATGGCCG GGCACTGGAG GAGCTCTCCT TCCAGCCCTC CCAGCCGCTC
    AGCAAATCGA GCTCTTCCCC CGAGCTGCAG ACTTTGCAGG AGATCCTCAA GGATGCAAAT
    GGCAGAGAAG TAACAAGGAG GCTGAGCACG GAAGTGAAGT CCAAATCTCA GTCTGGGAAC
    CTGGAAGGGG AAGGACTGGG CAGTTGGCTG AACAAGGGTG AGGATGCCAG AGCAGCAGGC
    TCGGGGGGGT TGGATGGCAG TGGCCCCACC ACCTCGCCAC GCTCCCCATC TGGGCACCGG
    CCCCGGGGGT ACACCATCTC TGACTCTGCC CCATCACGGA GAGGGAAGAG GATGGAGAGA
    GATGCCTTCA AGAGCAGAAC AGCAGCCACC AATGCTGAGA AAGTACCTGG AATAAACCCA
    AGCTTTGTGT TTTTACAATT GTACCATTCC CCGTTCTTTG GTGATGAAAA CAACAAGCCC
    CTCCTGTTGC CAAATGAGAC GTTCGAAAAA TCGGTGCAGC TCCTGGATCA AATTCCTTCG
    TATGACACCC ACAAGATCGC AGTGCTGTAC GTTGGAGAGG GCCAGAGCAA CAATGAAATC
    GCTATTCTGT CCAATGAACA CGGCTCCTAT CGCTACACCG AGTTCCTGAC AGGCTTAGGG
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCA GATAAAATCT ACCTCGGTGG CCTGGACGTG
    TGTGGGGAGG ATGGACAGTT TACCTACTGC TGGCACGATG ACATCATGCA AGCTATTTTC
    CACATTGCTA CACTGATGCC CACAAAGGAT TTGGATAAAT ATCGCTGTGA CAAGAAGAGG
    CACCTTGGGA ATGATTTTGT TTCTATTGTT TACAATGATT CTGGTGAGGA CTTCAAACTG
    GGAACAATAA AGGGTCAGTT CAATTTTGTG CATGTTATCA TAACCCCACT TGACTATGAC
    TGCAACCTGG TCACACTGCA GTGTCGAAAA GACATGGAGG GCCTTGTAGA CACGAGTGTT
    GCTAAGATCA TCTCTGACAA GAACCTGCCA TTCGTGGCCC GTCAGATGGC CCTGCATGCT
    AACATGGCTT CCCAGGTGCA CCACAGTCGA TCCAACCCTA CTGACACCTA CCCATCCAAA
    TGGATCGCTC GGCTGCGGCA CATCAAACGG CTGAGGCACC GGCTACGTGA AGAAACCCAG
    TACCAAACTC CTGGCCTTCC CCTGCAGATG CACCCGTCTG CTCCAACAAA GCCCTCTCCC
    CAGATGGCTC AGGACCCACC GCCGGCCTAC GAGACCGGTC AGAGGAAGCG GCTGATCTCC
    TCGGTGGATG ACTTCACTGA GTTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42378
    Clone ID Related Accession (Same CDS sequence) XM_015294452.1 , XM_015294452.2
    Accession Version XM_015294452.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tuberin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TCGGATACAG ACATCCATCA CTAGTTCCAG CTTGGGCTCT
    GCAGATGAAA ATTCAATGGC TCAGGCTGAT GACAACTTAA AAAATCTCCA TCTGGAACTC
    ACTGAGACTT GTCTGGATAT GATGGCCCGA TACGTCTTTT CCAACTTCAC TGCGGTGCCT
    AAGAGGTCTC CTGTGGGTGA GTTTCTGTTG GCTGGAGGAA GGACAAAGAC ATGGCTGGTT
    GGCAACAAGT TGGTGACCAT CACTACAAGT GTCGGAACAG GGACAAGGTC CTTGCTTGGC
    CTGGACTCTG GAGATCTCCA AAGCAGCACA GAATCAGGCT CAGACCCGGT TTTGCAAGTG
    AGACAAACTA AAGAAGCTCC AGCAAAGCTC GAATCTCAAG CTGGGCAGCA GGTTTGCAGA
    AGCTCTCGCA ACAGAGTCAG ATCCATGTCT GGTGGTCATG CCTTACGTGT TGGTGCCTTA
    GACAGCACAG CCTCCCACTT CCCTGGTGGC TCGGCTTCAC AAGGGACACA GAGTCCTCCT
    GCTCCTGCAC CTCGGTCAGA GAAGACTAAT CCTGCTCCAC AGACACCTCT GCAGAAAGAA
    AAAGCGAACT TAGCTGCATA TGTCCCACTG CTGACACAGG GCTGGGCAGA AATCCTTGTG
    CGCAGACCCA CAGGTAACAC AAGCTGGCTA ATGAGTCTGG AGAACCCGCT CAGTCCCTTT
    TCTTCAGACA TTAACAATAT GCCCCTGCAA GAGCTGTCCA ACGCACTCAT GGCTGCAGAG
    CGTTTTAAAG AGCACCGAGA AACAGCTCTC TACAAATCCC TGTCTGTCCC TTCCTCCAGC
    TTGGCCACTG GGACAGCCAA GCCTTCACTT CTCCAGCGTT CCAACACAGA GTCTGCGGTC
    GTGCTGGAGG AAGGGAGCCC CTTGGAAATG GAGCTGAAGG AAACTGAGTC CCCAGCTGAG
    TCTCCAGAGA GTGAAGATTT TGAGACGTCG TGTTCTGAGC AAGTCCTGAG CGAGGAAAGG
    TTTGGTAAAG CCCAGGAGTT TTACAGCAGG TCGTCCTCGA CCTCTAGCCA AGATGAAAAA
    TCTCTGAAGT CGGACGAGCT GGTGGAGGGC GGTATTCCCA TCGGGAGAGT GCTGCCAGCA
    GAGGATGGCC GGGCACTGGA GGAGCTCTCC TTCCAGCCCT CCCAGCCGCT CAGCAAATCG
    AGCTCTTCCC CCGAGCTGCA GACTTTGCAG GAGATCCTCA AGGATGCAAA TGGCAGAGAA
    GTAACAAGGA GGCTGAGCAC GGAAGTGAAG TCCAAATCTC AGTCTGGGAA CCTGGAAGGG
    GAAGGACTGG GCAGTTGGCT GAACAAGGGT GAGGATGCCA GAGCAGCAGG CTCGGGGGGG
    TTGGATGGCA GTGGCCCCAC CACCTCGCCA CGCTCCCCAT CTGGGCACCG GCCCCGGGGG
    TACACCATCT CTGACTCTGC CCCATCACGG AGAGGGAAGA GGATGGAGAG AGATGCCTTC
    AAGAGCAGAA CAGCAGCCAC CAATGCTGAG AAAGTACCTG GAATAAACCC AAGCTTTGTG
    TTTTTACAAT TGTACCATTC CCCGTTCTTT GGTGATGAAA ACAACAAGCC CCTCCTGTTG
    CCAAATGAGA CGTTCGAAAA ATCGGTGCAG CTCCTGGATC AAATTCCTTC GTATGACACC
    CACAAGATCG CAGTGCTGTA CGTTGGAGAG GGCCAGAGCA ACAATGAAAT CGCTATTCTG
    TCCAATGAAC ACGGCTCCTA TCGCTACACC GAGTTCCTGA CAGGCTTAGG GAAGCTCATT
    GAGCTCAAAG ACTGCCAGCC AGATAAAATC TACCTCGGTG GCCTGGACGT GTGTGGGGAG
    GATGGACAGT TTACCTACTG CTGGCACGAT GACATCATGC AAGCTATTTT CCACATTGCT
    ACACTGATGC CCACAAAGGA TTTGGATAAA TATCGCTGTG ACAAGAAGAG GCACCTTGGG
    AATGATTTTG TTTCTATTGT TTACAATGAT TCTGGTGAGG ACTTCAAACT GGGAACAATA
    AAGGGTCAGT TCAATTTTGT GCATGTTATC ATAACCCCAC TTGACTATGA CTGCAACCTG
    GTCACACTGC AGTGTCGAAA AGACATGGAG GGCCTTGTAG ACACGAGTGT TGCTAAGATC
    ATCTCTGACA AGAACCTGCC ATTCGTGGCC CGTCAGATGG CCCTGCATGC TAACATGGCT
    TCCCAGGTGC ACCACAGTCG ATCCAACCCT ACTGACACCT ACCCATCCAA ATGGATCGCT
    CGGCTGCGGC ACATCAAACG GCTGAGGCAC CGGCTACGTG AAGAAACCCA GTACCAAACT
    CCTGGCCTTC CCCTGCAGAT GCACCCGTCT GCTCCAACAA AGCCCTCTCC CCAGATGGCT
    CAGGACCCAC CGCCGGCCTA CGAGACCGGT CAGAGGAAGC GGCTGATCTC CTCGGTGGAT
    GACTTCACTG AGTTTGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149938.1
    CDS57..5357
    Translation

    Target ORF information:

    RefSeq Version XM_015294452.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TCGGATACAG ACATCCATCA CTAGTTCCAG CTTGGGCTCT
    GCAGATGAAA ATTCAATGGC TCAGGCTGAT GACAACTTAA AAAATCTCCA TCTGGAACTC
    ACTGAGACTT GTCTGGATAT GATGGCCCGA TACGTCTTTT CCAACTTCAC TGCGGTGCCT
    AAGAGGTCTC CTGTGGGTGA GTTTCTGTTG GCTGGAGGAA GGACAAAGAC ATGGCTGGTT
    GGCAACAAGT TGGTGACCAT CACTACAAGT GTCGGAACAG GGACAAGGTC CTTGCTTGGC
    CTGGACTCTG GAGATCTCCA AAGCAGCACA GAATCAGGCT CAGACCCGGT TTTGCAAGTG
    AGACAAACTA AAGAAGCTCC AGCAAAGCTC GAATCTCAAG CTGGGCAGCA GGTTTGCAGA
    AGCTCTCGCA ACAGAGTCAG ATCCATGTCT GGTGGTCATG CCTTACGTGT TGGTGCCTTA
    GACAGCACAG CCTCCCACTT CCCTGGTGGC TCGGCTTCAC AAGGGACACA GAGTCCTCCT
    GCTCCTGCAC CTCGGTCAGA GAAGACTAAT CCTGCTCCAC AGACACCTCT GCAGAAAGAA
    AAAGCGAACT TAGCTGCATA TGTCCCACTG CTGACACAGG GCTGGGCAGA AATCCTTGTG
    CGCAGACCCA CAGGTAACAC AAGCTGGCTA ATGAGTCTGG AGAACCCGCT CAGTCCCTTT
    TCTTCAGACA TTAACAATAT GCCCCTGCAA GAGCTGTCCA ACGCACTCAT GGCTGCAGAG
    CGTTTTAAAG AGCACCGAGA AACAGCTCTC TACAAATCCC TGTCTGTCCC TTCCTCCAGC
    TTGGCCACTG GGACAGCCAA GCCTTCACTT CTCCAGCGTT CCAACACAGA GTCTGCGGTC
    GTGCTGGAGG AAGGGAGCCC CTTGGAAATG GAGCTGAAGG AAACTGAGTC CCCAGCTGAG
    TCTCCAGAGA GTGAAGATTT TGAGACGTCG TGTTCTGAGC AAGTCCTGAG CGAGGAAAGG
    TTTGGTAAAG CCCAGGAGTT TTACAGCAGG TCGTCCTCGA CCTCTAGCCA AGATGAAAAA
    TCTCTGAAGT CGGACGAGCT GGTGGAGGGC GGTATTCCCA TCGGGAGAGT GCTGCCAGCA
    GAGGATGGCC GGGCACTGGA GGAGCTCTCC TTCCAGCCCT CCCAGCCGCT CAGCAAATCG
    AGCTCTTCCC CCGAGCTGCA GACTTTGCAG GAGATCCTCA AGGATGCAAA TGGCAGAGAA
    GTAACAAGGA GGCTGAGCAC GGAAGTGAAG TCCAAATCTC AGTCTGGGAA CCTGGAAGGG
    GAAGGACTGG GCAGTTGGCT GAACAAGGGT GAGGATGCCA GAGCAGCAGG CTCGGGGGGG
    TTGGATGGCA GTGGCCCCAC CACCTCGCCA CGCTCCCCAT CTGGGCACCG GCCCCGGGGG
    TACACCATCT CTGACTCTGC CCCATCACGG AGAGGGAAGA GGATGGAGAG AGATGCCTTC
    AAGAGCAGAA CAGCAGCCAC CAATGCTGAG AAAGTACCTG GAATAAACCC AAGCTTTGTG
    TTTTTACAAT TGTACCATTC CCCGTTCTTT GGTGATGAAA ACAACAAGCC CCTCCTGTTG
    CCAAATGAGA CGTTCGAAAA ATCGGTGCAG CTCCTGGATC AAATTCCTTC GTATGACACC
    CACAAGATCG CAGTGCTGTA CGTTGGAGAG GGCCAGAGCA ACAATGAAAT CGCTATTCTG
    TCCAATGAAC ACGGCTCCTA TCGCTACACC GAGTTCCTGA CAGGCTTAGG GAAGCTCATT
    GAGCTCAAAG ACTGCCAGCC AGATAAAATC TACCTCGGTG GCCTGGACGT GTGTGGGGAG
    GATGGACAGT TTACCTACTG CTGGCACGAT GACATCATGC AAGCTATTTT CCACATTGCT
    ACACTGATGC CCACAAAGGA TTTGGATAAA TATCGCTGTG ACAAGAAGAG GCACCTTGGG
    AATGATTTTG TTTCTATTGT TTACAATGAT TCTGGTGAGG ACTTCAAACT GGGAACAATA
    AAGGGTCAGT TCAATTTTGT GCATGTTATC ATAACCCCAC TTGACTATGA CTGCAACCTG
    GTCACACTGC AGTGTCGAAA AGACATGGAG GGCCTTGTAG ACACGAGTGT TGCTAAGATC
    ATCTCTGACA AGAACCTGCC ATTCGTGGCC CGTCAGATGG CCCTGCATGC TAACATGGCT
    TCCCAGGTGC ACCACAGTCG ATCCAACCCT ACTGACACCT ACCCATCCAA ATGGATCGCT
    CGGCTGCGGC ACATCAAACG GCTGAGGCAC CGGCTACGTG AAGAAACCCA GTACCAAACT
    CCTGGCCTTC CCCTGCAGAT GCACCCGTCT GCTCCAACAA AGCCCTCTCC CCAGATGGCT
    CAGGACCCAC CGCCGGCCTA CGAGACCGGT CAGAGGAAGC GGCTGATCTC CTCGGTGGAT
    GACTTCACTG AGTTTGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42378
    Clone ID Related Accession (Same CDS sequence) XM_015294452.1 , XM_015294452.2
    Accession Version XM_015294452.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tuberin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006101.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TCGGATACAG ACATCCATCA CTAGTTCCAG CTTGGGCTCT
    GCAGATGAAA ATTCAATGGC TCAGGCTGAT GACAACTTAA AAAATCTCCA TCTGGAACTC
    ACTGAGACTT GTCTGGATAT GATGGCCCGA TACGTCTTTT CCAACTTCAC TGCGGTGCCT
    AAGAGGTCTC CTGTGGGTGA GTTTCTGTTG GCTGGAGGAA GGACAAAGAC ATGGCTGGTT
    GGCAACAAGT TGGTGACCAT CACTACAAGT GTCGGAACAG GGACAAGGTC CTTGCTTGGC
    CTGGACTCTG GAGATCTCCA AAGCAGCACA GAATCAGGCT CAGACCCGGT TTTGCAAGTG
    AGACAAACTA AAGAAGCTCC AGCAAAGCTC GAATCTCAAG CTGGGCAGCA GGTTTGCAGA
    AGCTCTCGCA ACAGAGTCAG ATCCATGTCT GGTGGTCATG CCTTACGTGT TGGTGCCTTA
    GACAGCACAG CCTCCCACTT CCCTGGTGGC TCGGCTTCAC AAGGGACACA GAGTCCTCCT
    GCTCCTGCAC CTCGGTCAGA GAAGACTAAT CCTGCTCCAC AGACACCTCT GCAGAAAGAA
    AAAGCGAACT TAGCTGCATA TGTCCCACTG CTGACACAGG GCTGGGCAGA AATCCTTGTG
    CGCAGACCCA CAGGTAACAC AAGCTGGCTA ATGAGTCTGG AGAACCCGCT CAGTCCCTTT
    TCTTCAGACA TTAACAATAT GCCCCTGCAA GAGCTGTCCA ACGCACTCAT GGCTGCAGAG
    CGTTTTAAAG AGCACCGAGA AACAGCTCTC TACAAATCCC TGTCTGTCCC TTCCTCCAGC
    TTGGCCACTG GGACAGCCAA GCCTTCACTT CTCCAGCGTT CCAACACAGA GTCTGCGGTC
    GTGCTGGAGG AAGGGAGCCC CTTGGAAATG GAGCTGAAGG AAACTGAGTC CCCAGCTGAG
    TCTCCAGAGA GTGAAGATTT TGAGACGTCG TGTTCTGAGC AAGTCCTGAG CGAGGAAAGG
    TTTGGTAAAG CCCAGGAGTT TTACAGCAGG TCGTCCTCGA CCTCTAGCCA AGATGAAAAA
    TCTCTGAAGT CGGACGAGCT GGTGGAGGGC GGTATTCCCA TCGGGAGAGT GCTGCCAGCA
    GAGGATGGCC GGGCACTGGA GGAGCTCTCC TTCCAGCCCT CCCAGCCGCT CAGCAAATCG
    AGCTCTTCCC CCGAGCTGCA GACTTTGCAG GAGATCCTCA AGGATGCAAA TGGCAGAGAA
    GTAACAAGGA GGCTGAGCAC GGAAGTGAAG TCCAAATCTC AGTCTGGGAA CCTGGAAGGG
    GAAGGACTGG GCAGTTGGCT GAACAAGGGT GAGGATGCCA GAGCAGCAGG CTCGGGGGGG
    TTGGATGGCA GTGGCCCCAC CACCTCGCCA CGCTCCCCAT CTGGGCACCG GCCCCGGGGG
    TACACCATCT CTGACTCTGC CCCATCACGG AGAGGGAAGA GGATGGAGAG AGATGCCTTC
    AAGAGCAGAA CAGCAGCCAC CAATGCTGAG AAAGTACCTG GAATAAACCC AAGCTTTGTG
    TTTTTACAAT TGTACCATTC CCCGTTCTTT GGTGATGAAA ACAACAAGCC CCTCCTGTTG
    CCAAATGAGA CGTTCGAAAA ATCGGTGCAG CTCCTGGATC AAATTCCTTC GTATGACACC
    CACAAGATCG CAGTGCTGTA CGTTGGAGAG GGCCAGAGCA ACAATGAAAT CGCTATTCTG
    TCCAATGAAC ACGGCTCCTA TCGCTACACC GAGTTCCTGA CAGGCTTAGG GAAGCTCATT
    GAGCTCAAAG ACTGCCAGCC AGATAAAATC TACCTCGGTG GCCTGGACGT GTGTGGGGAG
    GATGGACAGT TTACCTACTG CTGGCACGAT GACATCATGC AAGCTATTTT CCACATTGCT
    ACACTGATGC CCACAAAGGA TTTGGATAAA TATCGCTGTG ACAAGAAGAG GCACCTTGGG
    AATGATTTTG TTTCTATTGT TTACAATGAT TCTGGTGAGG ACTTCAAACT GGGAACAATA
    AAGGGTCAGT TCAATTTTGT GCATGTTATC ATAACCCCAC TTGACTATGA CTGCAACCTG
    GTCACACTGC AGTGTCGAAA AGACATGGAG GGCCTTGTAG ACACGAGTGT TGCTAAGATC
    ATCTCTGACA AGAACCTGCC ATTCGTGGCC CGTCAGATGG CCCTGCATGC TAACATGGCT
    TCCCAGGTGC ACCACAGTCG ATCCAACCCT ACTGACACCT ACCCATCCAA ATGGATCGCT
    CGGCTGCGGC ACATCAAACG GCTGAGGCAC CGGCTACGTG AAGAAACCCA GTACCAAACT
    CCTGGCCTTC CCCTGCAGAT GCACCCGTCT GCTCCAACAA AGCCCTCTCC CCAGATGGCT
    CAGGACCCAC CGCCGGCCTA CGAGACCGGT CAGAGGAAGC GGCTGATCTC CTCGGTGGAT
    GACTTCACTG AGTTTGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015149938.1
    CDS57..5357
    Translation

    Target ORF information:

    RefSeq Version XM_015294452.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015294452.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCAAAGC CTCAAAGCAA GGATTCGGGG CTGAAGGAGA AGTTCAAGAA TCTCTTGGGG 
    CTGGGAACAT CCCGGGGAAG TTCAAAGTCA TCAGAGGGCA AGCAAACGGA ATTCATCATC
    ACTGCAGAAA TACTCAAGGA ACTGAGCATA GAATGTGGAT TAAGTAATAG GATACGAGCA
    ATCAGCCAAA TCTGTGAAGT GGCAAAAACC AAAAAAATTG AAGAGCATGC AGTGGAAGCA
    ATATGGAAGG TGGTAGCTGA TATGCTCCAG CCAGAGCGCC CAGCTGAAGC CAGACATGCT
    GTTCTTCATC TCCTAAAGTC AATCATTCAA GGACAGGGTG AGAGATTAGG GATCCTTAGA
    GCACATTTTT TTAAGGTTAT TAAGGACTAT CCTTCCAATG AAGACTTGCA TGAACGGCTT
    GAAGTTTTTA AGGCTCTAAC AGAGAATGGA CGATACATCA CTTACTTAGA AGAAGAGTTA
    GCTGACTTCG TGCTACAGTG GATGGATGTT GGTTTGTCTT CAGAGTTCCT TTTGGTGTTA
    GTGAACCTGG TGAAATTCAA TAGCTGCTAT TTGGAAGACT ACGTAGCTGA CATGGTCCAC
    AAGATATGCA TCTTGTGTAT TCAGACTTCA TCATCTGTGG ACATAGAGAT TTCTTTGCAA
    GTTTTAGATG CTGTTGTTTG CTATAACTGT CTGCCGTCAG AGAACCTGCC AGTATTTATC
    ATCACTTTGT GCCGTACCAT CAATGTGAAG GAGCTCTGTG AGCCATGCTG GAAGCTTATG
    CGCAACCTTT TGGGAACTCA TTTGGGACAC AGTGCAATTT ACAACATGTG CAGGATCATG
    GAAGACAGAG CTTACATGGC AGATGCAGCC CTGCTGAGAG GAGCGGTGTT CTTTGTTGGG
    ATGGCTCTCT GGGGTGCTCA TCGCCTCAAT TCACTCAAGA ATTCCCCAAC TTCAGTGCTG
    CCTTCGTTCC TCAAGGCCAT GACGTGTCCC AATGCAGTTG TATCTTATGA GATAGTTCTG
    TCCATCACCC GTCTAATAAA GAAGTATGGG AAAGAACTGC AAGCTGTAAC CTGGGATATC
    CTCTTGGACA TCATGGAGCG ATTACTGCAA CAGTTGCAGA GTTTAGAGAG CCAAGAACTT
    AAGTCCATTG TACATGATCT TTTGACTACA GTAGAAGAAC TCTGTGACCA GAATGAATTT
    CATGGTTCTG AGGAGAGATT TTTTGAGCTG GTGGAAAGAT GCGCTGATCA GAGACCGGAA
    TCTTCCGTAT TAAACTTGAT AGCATACAGA GCTCAATCCA TTCATCCTGC CAAAGATGGC
    TGGATTCACA ACCTGCAGAT GTTAATGGAG AGATTCTTCA GGAACGAAAG TCGTAGTGCT
    GTTCGTATTA AAGTTCTGGA TGTCTTGTCC TTTGTACTGA GTATTAACAG ACAGTTCTAT
    GAGGAAGAGC TGATAAACTT GGTTGTGATC TCTCAGCTGG CTCACATTCC AGAGGATAAA
    GACCATCAAG TTCGAAAACT TGCCACTCAG CTACTTGTAG ACCTTGCTGA AGGCTGCAAC
    ACACATCACT TCAACAGCTT GCTTGATATC ATAGAAAAGG TGGCTGCGCA TTCTCTCTCA
    TCTCCATCTG AACTGGAAGA GAGGGATTTG CTATCATATT CAGCTTCTTT GGAGGACGTG
    AAGACAGCAG TTCTTGGACT TCTGATAATT CTTCAGACAA AACTCTACAG CTTACCTTCC
    AGCCATGCCA TGCGGGTGTA TGAGATGCTG ATTCACCACA TCCAGCGCCA CTATATGTAT
    GCGTACAGCC TTGCTGTTGC TAGCAGCATC AGACTGCAGG TGTTTGATTT CCTCCTGATG
    CTTCGAGCTG ACTCCCTCCA CCGTCTTGGC CTTTCTAACA AGGATGGGGC AGTGAGATTC
    AGCCCTTACT GTCTGTGTGA TTTTGTAGAA GCAGAGAAGA GAGCTTCTGA GAAAAAGCCT
    ACTGGTACGC TCTCTCCACC TTCAGGAAGT CCCAGTGTGC CTTCCCAGAA CAGCACCATT
    CGAATAGGGC ATTTGCCATA CTCAATGGTC TTTGGTGTCC TTCTGCAGTG TTTGAAGCAA
    GAGACAGACT GGAAGGTGTT GAAGTTGGTC CTCAACAAAC TACCAGAATC ACTCCGCTAT
    AAAGTGCTGT TTTTAACCTC TCCTTGTAAC ATAGACCTCC TGGCATCTGC ACTGAGCTAC
    ATGCTCACAG ACAAGAAGAC AACCGACAGG CTCCATGGTA CCCCAGAAGG CTTTTCCCGC
    ACCGATTTGC ACCTGGCTGT AGTTCCAGTA CTGACAGCGT TGATATCTTA TCATAATTAT
    CTGGACAAAG CTAAACAGCG TGAAATAGTG TACTGCCTGG AGCATGGCCT TATTTATCGC
    TGTGCAAACC AGTGCGTTGT GGCACTCTCT GTCTGCAGCG TTGAGATGCC CGACATCATC
    ATAAAGGCAC TTCCAGTCTT AATAGTCAAA TTAACCCACA TATCTGCTAC AGCCAACATG
    GCAATCCCTC TCTTAGAATT TCTTTCAACT CTAGCAAGGC TGCCTCACCT CTACAGGAAC
    TTTGCTGCAG AGCAGTATGC CAGTGTGTTT GCCATCTCCC TGCCATACAC AAACCCTTCC
    AAGTTTAACC AGTACATTGT TTGCCTGGCC CACCACGTGA TAGCTATGTG GTTCATTCGG
    TGTCGTCTTC CCTTCCGAAA GGACTTTGTT CCCTATATTA CAAAGGGTTT GCGCTCCAAT
    GTGCTGCTCT CCTTTGATGA CACACCTGAG AAGGACAGTT TCAGGGCTCG TAGTACAAGC
    CTCAATGAGA GGCCAAAAAG TCGGATACAG ACATCCATCA CTAGTTCCAG CTTGGGCTCT
    GCAGATGAAA ATTCAATGGC TCAGGCTGAT GACAACTTAA AAAATCTCCA TCTGGAACTC
    ACTGAGACTT GTCTGGATAT GATGGCCCGA TACGTCTTTT CCAACTTCAC TGCGGTGCCT
    AAGAGGTCTC CTGTGGGTGA GTTTCTGTTG GCTGGAGGAA GGACAAAGAC ATGGCTGGTT
    GGCAACAAGT TGGTGACCAT CACTACAAGT GTCGGAACAG GGACAAGGTC CTTGCTTGGC
    CTGGACTCTG GAGATCTCCA AAGCAGCACA GAATCAGGCT CAGACCCGGT TTTGCAAGTG
    AGACAAACTA AAGAAGCTCC AGCAAAGCTC GAATCTCAAG CTGGGCAGCA GGTTTGCAGA
    AGCTCTCGCA ACAGAGTCAG ATCCATGTCT GGTGGTCATG CCTTACGTGT TGGTGCCTTA
    GACAGCACAG CCTCCCACTT CCCTGGTGGC TCGGCTTCAC AAGGGACACA GAGTCCTCCT
    GCTCCTGCAC CTCGGTCAGA GAAGACTAAT CCTGCTCCAC AGACACCTCT GCAGAAAGAA
    AAAGCGAACT TAGCTGCATA TGTCCCACTG CTGACACAGG GCTGGGCAGA AATCCTTGTG
    CGCAGACCCA CAGGTAACAC AAGCTGGCTA ATGAGTCTGG AGAACCCGCT CAGTCCCTTT
    TCTTCAGACA TTAACAATAT GCCCCTGCAA GAGCTGTCCA ACGCACTCAT GGCTGCAGAG
    CGTTTTAAAG AGCACCGAGA AACAGCTCTC TACAAATCCC TGTCTGTCCC TTCCTCCAGC
    TTGGCCACTG GGACAGCCAA GCCTTCACTT CTCCAGCGTT CCAACACAGA GTCTGCGGTC
    GTGCTGGAGG AAGGGAGCCC CTTGGAAATG GAGCTGAAGG AAACTGAGTC CCCAGCTGAG
    TCTCCAGAGA GTGAAGATTT TGAGACGTCG TGTTCTGAGC AAGTCCTGAG CGAGGAAAGG
    TTTGGTAAAG CCCAGGAGTT TTACAGCAGG TCGTCCTCGA CCTCTAGCCA AGATGAAAAA
    TCTCTGAAGT CGGACGAGCT GGTGGAGGGC GGTATTCCCA TCGGGAGAGT GCTGCCAGCA
    GAGGATGGCC GGGCACTGGA GGAGCTCTCC TTCCAGCCCT CCCAGCCGCT CAGCAAATCG
    AGCTCTTCCC CCGAGCTGCA GACTTTGCAG GAGATCCTCA AGGATGCAAA TGGCAGAGAA
    GTAACAAGGA GGCTGAGCAC GGAAGTGAAG TCCAAATCTC AGTCTGGGAA CCTGGAAGGG
    GAAGGACTGG GCAGTTGGCT GAACAAGGGT GAGGATGCCA GAGCAGCAGG CTCGGGGGGG
    TTGGATGGCA GTGGCCCCAC CACCTCGCCA CGCTCCCCAT CTGGGCACCG GCCCCGGGGG
    TACACCATCT CTGACTCTGC CCCATCACGG AGAGGGAAGA GGATGGAGAG AGATGCCTTC
    AAGAGCAGAA CAGCAGCCAC CAATGCTGAG AAAGTACCTG GAATAAACCC AAGCTTTGTG
    TTTTTACAAT TGTACCATTC CCCGTTCTTT GGTGATGAAA ACAACAAGCC CCTCCTGTTG
    CCAAATGAGA CGTTCGAAAA ATCGGTGCAG CTCCTGGATC AAATTCCTTC GTATGACACC
    CACAAGATCG CAGTGCTGTA CGTTGGAGAG GGCCAGAGCA ACAATGAAAT CGCTATTCTG
    TCCAATGAAC ACGGCTCCTA TCGCTACACC GAGTTCCTGA CAGGCTTAGG GAAGCTCATT
    GAGCTCAAAG ACTGCCAGCC AGATAAAATC TACCTCGGTG GCCTGGACGT GTGTGGGGAG
    GATGGACAGT TTACCTACTG CTGGCACGAT GACATCATGC AAGCTATTTT CCACATTGCT
    ACACTGATGC CCACAAAGGA TTTGGATAAA TATCGCTGTG ACAAGAAGAG GCACCTTGGG
    AATGATTTTG TTTCTATTGT TTACAATGAT TCTGGTGAGG ACTTCAAACT GGGAACAATA
    AAGGGTCAGT TCAATTTTGT GCATGTTATC ATAACCCCAC TTGACTATGA CTGCAACCTG
    GTCACACTGC AGTGTCGAAA AGACATGGAG GGCCTTGTAG ACACGAGTGT TGCTAAGATC
    ATCTCTGACA AGAACCTGCC ATTCGTGGCC CGTCAGATGG CCCTGCATGC TAACATGGCT
    TCCCAGGTGC ACCACAGTCG ATCCAACCCT ACTGACACCT ACCCATCCAA ATGGATCGCT
    CGGCTGCGGC ACATCAAACG GCTGAGGCAC CGGCTACGTG AAGAAACCCA GTACCAAACT
    CCTGGCCTTC CCCTGCAGAT GCACCCGTCT GCTCCAACAA AGCCCTCTCC CCAGATGGCT
    CAGGACCCAC CGCCGGCCTA CGAGACCGGT CAGAGGAAGC GGCTGATCTC CTCGGTGGAT
    GACTTCACTG AGTTTGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.