TSC2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005621719.3	XP_005621776.1	tuberin isoform X5
XM_022420250.1	XP_022275958.1	tuberin isoform X8
XM_022420252.1	XP_022275960.1	tuberin isoform X10
XM_005621718.2	XP_005621775.1	tuberin isoform X4
XM_005621719.2	XP_005621776.1	tuberin isoform X5
XM_005621716.2	XP_005621773.1	tuberin isoform X2
XM_005621717.2	XP_005621774.1	tuberin isoform X3
XM_005621720.2	XP_005621777.1	tuberin isoform X6
XM_005621716.3	XP_005621773.1	tuberin isoform X2
XM_005621715.2	XP_005621772.1	tuberin isoform X1
XM_005621717.3	XP_005621774.1	tuberin isoform X3
XM_014114692.1	XP_013970167.1	tuberin isoform X7
XM_005621715.3	XP_005621772.1	tuberin isoform X1
XM_005621718.3	XP_005621775.1	tuberin isoform X4
XM_005621720.3	XP_005621777.1	tuberin isoform X6
XM_014114692.2	XP_013970167.1	tuberin isoform X7
XM_022420251.1	XP_022275959.1	tuberin isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe289103	XM_022420250.1 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe289104	XM_022420251.1 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe289105	XM_022420252.1 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe37159	XM_014114692.2 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe08328	XM_005621715.3 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08329	XM_005621716.3 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08331	XM_005621717.3 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08333	XM_005621718.3 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08334	XM_005621719.3 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08335	XM_005621720.3 Latest version!	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OCe08334	XM_005621719.2	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08329	XM_005621716.2	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08331	XM_005621717.2	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08328	XM_005621715.2	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08333	XM_005621718.2	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe08335	XM_005621720.2	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe37159	XM_014114692.1	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

TSC2 ( XM_022420250.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275958.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe289103
Clone ID Related Accession (Same CDS sequence)	XM_022420250.1
Accession Version	XM_022420250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATCG GCCTGTCCTC CGAATTCCTC CTGGTGCTTG TGAACTTGGT CAAGTTCAAC 
AGCTGTTACC TGGATGAGTA CATTGCATCA ATGGTCCACA TGATCTGCCT GCTGTGCGTC 
CAGACCGTGT CATCCGTGGA CATAGAGGTC TCCCTGCAGG TGCTGGACGC CGTCGTCTGC 
TACAACTGCC TGCCGGCTGA GAGCCTCCCC CTGTTCATCG TCACCCTGTG CCGCACCATC 
AACGTGAAGG AGCTCTGTGA GCCTTGCTGG AAGCTGATGC GGAACCTGCT GGGCACCCAC 
CTGGGCCACA GTGCCATCTA CAACATGTGT CGCATCATGG AGGACAGAGC CTACATGGAA 
GATGCCCCAC TGCTGAGAGG GGCTGTGTTC TTTGTGGGGA TGGCCCTTTG GGGAGCACAC 
CGACTCTACT CTCTCAAGAA CTCCCCAACG TCTGTGTTGC CGTCGTTTTA TGAGGCTATG 
ACCTGTCCGA ATGAAGTGGT GTCCTATGAA ATCGTCCTCT CTATAACCAG ACTCATCAAG 
AAGTACCGGA GGGAGCTGCA AGCTGTGACC TGGGACATCC TACTGAACAT CATGGAGCGC 
CTCCTGCACC AGCTCCAGAG CCTGGACAGC CCGGAGCTCA GAGCCATCGT GCACGACCTG 
CTGACCACAG TGGAAGAGCT GTGTGATCAG AATGAGTTCC ACGGCTCTCA GGAGAGATAC 
TTTGAGCTGG TTGAGCGATG TGCAGACCAG AGGCCGGAGT CTTCCCTCTT AAACTTAATA 
ACGTACCGGG CTCAGTCAAT CCACCCGGCG AAGGATGGCT GGATTCAGAA CCTGCAGTTA 
CTGATGGAGA GGTTCTTCAG GAACGAATCT CGCAGTGCTG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTATG AGGAGGAGCT GATCAGCTCG 
GTGGTCATCT CACAGCTCTC CCATGTCCCG GAGGACAAAG ACCCCCAAGT CCGGAAACTG 
GCCACCCAGT TGCTGGTGGA CCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGTCTG 
TTGGACATTG TGGAAAAGGT GATTGCCCGC TCTCTCTCTC CACCCCCTGA GCTGGAGGAA 
AGAGACGTGG CGGTGTACTC GGCCTCCTTA GAGGATGTGA AGACTGCTGT CCTGGGGCTC 
CTGGTCATCC TTCAGACCAA GCTGTACGCC TTGCCTGCCA GCCATGCGAT GCGCGTGTAC 
GAGACACTCG TCAGCCACAT CCAGCTCCAC TACAAGCATA GCTATACCCT GCCCATTGCC 
AGTAGCATCC GGCTGCAGGC TTTTGACTTT CTACTGCTGC TGAGGGCCGA CTCGCTGCAC 
CGCCTCGGCC TGCCCAGCAA GGACGGGGCC GTGAGGTTCA GCCCTTACTG CGTCTGCGAT 
GCCATGGAGC CAGAGAGAGG CTCAGAGAAG AAGGCCAGTG GCCCCCTGTC ACCTCCCACG 
GGGCCTCCTG GTCCTGCGCC CGCAGGCCCC GCCGTGCGGC TTGGCTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTCCTGCT GCAGTGCCTG AAGCTGGAGG TGGACTGGAA GGTACTGAAG 
CTCGTGCTTA GCAAGCTGCC AGAGTCCCTG CGTTATAAGG TGCTCATCTT CACCTCGCCC 
TGCAGCGTGG ACCAGCTGTC CTCCGCCCTC TGCTCCATGC TCTCAGGTCC CAGAACTCTG 
GAGCGGCTCC GAGGCACCCC GGAAGGGTTC TCCAGAACCG ACCTGCACCT GGCTGTGGTC 
CCTGTGCTGA CGGCGCTCAT CTCTTACCAT AACTACCTGG ACAAAACCAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTGATC TACCGCTGCG CCAGCCAGTG TGTGGTGGCC 
CTGGCCATCT GCAGCGTGGA GATGCCTGAC ATCATCATCA AGGCGCTTCC CGTCCTGGTC 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGTATGGCCA TTCCACTCCT CGAGTTCCTG 
TCAACGCTGG CCAGGTTGCC CCACCTGTAC AGGAACTTCG CAGCAGAGCA GTACGCGAGT 
GTGTTTGCCA TCTCCCTGCC CTACACCAAT CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ATGTCATAGC CATGTGGTTC ATTAGATGCC GCCTGCCCTT CCGGAAGGAT 
TTTGTTCCTT ATATCACTAA GGGCCTGCGC TCCAACGTCC TCCTGTCTTT TGATGACACC 
CCCGAGAAGG ACAGTTTCAG AGCACGAAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAATTCAAA 
GAGAGCTCTG CCGCCGAGGC CTTCCGATGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACGTC CCTCACAAGC GCCAGCTTGG GGTCTGCTGA CGAGAACTCG 
ATGGCCCAGG CTGATGACAA CTTGAAAAAT CTCCACCTGG AGCTCACGGA AACATGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACAGCAG TCCCCAAGAG GTCCCCGGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGCGTGGG GACGGGGACC CGGTCGCTGC TGGGCTTGGA CTCAGGAGAT 
CAGCAAGGTG GCCCCGAGTT GAGCTCGGAC CCTGGCATGC ATGTGAGACA GGCAAAGGAG 
GCACCTGCCA AGCTGGAGTC GCAGGCTGGG CAGCAGGTGT GCCGCGGCGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCACGGTCTT CGTGTTGGCG CCCTGGACAC TCCAGCCTCC 
CACTTGCCGG GTGGCCCCAC TTCCCCAGGC ACGCAGGCAG CGCTGGCCAG CAAGTCTGAC 
AGGGCCTCGG CTGGCACCCG GTTCCCAGCA CAGGAGAAGA CGAGCCTGGC AGCCTACGTA 
CCGCTGCTGA CCCAGGGCTG GGCGGAGATC TTGGTCCGGA GGCCTACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCGT CGGACATCAA CAACATGCCT 
CTGCAGGAGC TGTCCAACGC GCTCATGGCC GCTGAGCGCT TCAAGGAGCG TCGTGACACG 
GCTCTGTACA AGTCGCTGTC GGTGCCTGCC GCAGGCTCGG CCAAGCCCCC TCCGCCCCCG 
AGATCTAACA CGGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGTTGCCA AGGAAAGCTG 
CACAGGAGCA TTTCCTGGGC AGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG 
ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC 
CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG 
AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC 
GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC 
AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT 
GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG 
GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC 
GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC 
ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG 
AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC 
CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC 
AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG 
CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT 
GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC 
CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA 
GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA 
CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC 
AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG 
GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT 
GACTTCACGG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275958.1
CDS	585..5525
Translation	MDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_022420250.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420250.1

ORF Insert Sequence:

ATGGACATCG GCCTGTCCTC CGAATTCCTC CTGGTGCTTG TGAACTTGGT CAAGTTCAAC 
AGCTGTTACC TGGATGAGTA CATTGCATCA ATGGTCCACA TGATCTGCCT GCTGTGCGTC 
CAGACCGTGT CATCCGTGGA CATAGAGGTC TCCCTGCAGG TGCTGGACGC CGTCGTCTGC 
TACAACTGCC TGCCGGCTGA GAGCCTCCCC CTGTTCATCG TCACCCTGTG CCGCACCATC 
AACGTGAAGG AGCTCTGTGA GCCTTGCTGG AAGCTGATGC GGAACCTGCT GGGCACCCAC 
CTGGGCCACA GTGCCATCTA CAACATGTGT CGCATCATGG AGGACAGAGC CTACATGGAA 
GATGCCCCAC TGCTGAGAGG GGCTGTGTTC TTTGTGGGGA TGGCCCTTTG GGGAGCACAC 
CGACTCTACT CTCTCAAGAA CTCCCCAACG TCTGTGTTGC CGTCGTTTTA TGAGGCTATG 
ACCTGTCCGA ATGAAGTGGT GTCCTATGAA ATCGTCCTCT CTATAACCAG ACTCATCAAG 
AAGTACCGGA GGGAGCTGCA AGCTGTGACC TGGGACATCC TACTGAACAT CATGGAGCGC 
CTCCTGCACC AGCTCCAGAG CCTGGACAGC CCGGAGCTCA GAGCCATCGT GCACGACCTG 
CTGACCACAG TGGAAGAGCT GTGTGATCAG AATGAGTTCC ACGGCTCTCA GGAGAGATAC 
TTTGAGCTGG TTGAGCGATG TGCAGACCAG AGGCCGGAGT CTTCCCTCTT AAACTTAATA 
ACGTACCGGG CTCAGTCAAT CCACCCGGCG AAGGATGGCT GGATTCAGAA CCTGCAGTTA 
CTGATGGAGA GGTTCTTCAG GAACGAATCT CGCAGTGCTG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTATG AGGAGGAGCT GATCAGCTCG 
GTGGTCATCT CACAGCTCTC CCATGTCCCG GAGGACAAAG ACCCCCAAGT CCGGAAACTG 
GCCACCCAGT TGCTGGTGGA CCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGTCTG 
TTGGACATTG TGGAAAAGGT GATTGCCCGC TCTCTCTCTC CACCCCCTGA GCTGGAGGAA 
AGAGACGTGG CGGTGTACTC GGCCTCCTTA GAGGATGTGA AGACTGCTGT CCTGGGGCTC 
CTGGTCATCC TTCAGACCAA GCTGTACGCC TTGCCTGCCA GCCATGCGAT GCGCGTGTAC 
GAGACACTCG TCAGCCACAT CCAGCTCCAC TACAAGCATA GCTATACCCT GCCCATTGCC 
AGTAGCATCC GGCTGCAGGC TTTTGACTTT CTACTGCTGC TGAGGGCCGA CTCGCTGCAC 
CGCCTCGGCC TGCCCAGCAA GGACGGGGCC GTGAGGTTCA GCCCTTACTG CGTCTGCGAT 
GCCATGGAGC CAGAGAGAGG CTCAGAGAAG AAGGCCAGTG GCCCCCTGTC ACCTCCCACG 
GGGCCTCCTG GTCCTGCGCC CGCAGGCCCC GCCGTGCGGC TTGGCTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTCCTGCT GCAGTGCCTG AAGCTGGAGG TGGACTGGAA GGTACTGAAG 
CTCGTGCTTA GCAAGCTGCC AGAGTCCCTG CGTTATAAGG TGCTCATCTT CACCTCGCCC 
TGCAGCGTGG ACCAGCTGTC CTCCGCCCTC TGCTCCATGC TCTCAGGTCC CAGAACTCTG 
GAGCGGCTCC GAGGCACCCC GGAAGGGTTC TCCAGAACCG ACCTGCACCT GGCTGTGGTC 
CCTGTGCTGA CGGCGCTCAT CTCTTACCAT AACTACCTGG ACAAAACCAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTGATC TACCGCTGCG CCAGCCAGTG TGTGGTGGCC 
CTGGCCATCT GCAGCGTGGA GATGCCTGAC ATCATCATCA AGGCGCTTCC CGTCCTGGTC 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGTATGGCCA TTCCACTCCT CGAGTTCCTG 
TCAACGCTGG CCAGGTTGCC CCACCTGTAC AGGAACTTCG CAGCAGAGCA GTACGCGAGT 
GTGTTTGCCA TCTCCCTGCC CTACACCAAT CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ATGTCATAGC CATGTGGTTC ATTAGATGCC GCCTGCCCTT CCGGAAGGAT 
TTTGTTCCTT ATATCACTAA GGGCCTGCGC TCCAACGTCC TCCTGTCTTT TGATGACACC 
CCCGAGAAGG ACAGTTTCAG AGCACGAAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAATTCAAA 
GAGAGCTCTG CCGCCGAGGC CTTCCGATGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACGTC CCTCACAAGC GCCAGCTTGG GGTCTGCTGA CGAGAACTCG 
ATGGCCCAGG CTGATGACAA CTTGAAAAAT CTCCACCTGG AGCTCACGGA AACATGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACAGCAG TCCCCAAGAG GTCCCCGGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACTGTGACGA CGAGCGTGGG GACGGGGACC CGGTCGCTGC TGGGCTTGGA CTCAGGAGAT 
CAGCAAGGTG GCCCCGAGTT GAGCTCGGAC CCTGGCATGC ATGTGAGACA GGCAAAGGAG 
GCACCTGCCA AGCTGGAGTC GCAGGCTGGG CAGCAGGTGT GCCGCGGCGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCACGGTCTT CGTGTTGGCG CCCTGGACAC TCCAGCCTCC 
CACTTGCCGG GTGGCCCCAC TTCCCCAGGC ACGCAGGCAG CGCTGGCCAG CAAGTCTGAC 
AGGGCCTCGG CTGGCACCCG GTTCCCAGCA CAGGAGAAGA CGAGCCTGGC AGCCTACGTA 
CCGCTGCTGA CCCAGGGCTG GGCGGAGATC TTGGTCCGGA GGCCTACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCGT CGGACATCAA CAACATGCCT 
CTGCAGGAGC TGTCCAACGC GCTCATGGCC GCTGAGCGCT TCAAGGAGCG TCGTGACACG 
GCTCTGTACA AGTCGCTGTC GGTGCCTGCC GCAGGCTCGG CCAAGCCCCC TCCGCCCCCG 
AGATCTAACA CGGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGTTGCCA AGGAAAGCTG 
CACAGGAGCA TTTCCTGGGC AGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG 
ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC 
CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG 
AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC 
GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC 
AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT 
GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG 
GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC 
GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC 
ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG 
AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC 
CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC 
AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG 
CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT 
GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC 
CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA 
GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA 
CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC 
AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG 
GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT 
GACTTCACGG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_022420251.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275959.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe289104
Clone ID Related Accession (Same CDS sequence)	XM_022420251.1
Accession Version	XM_022420251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC 
GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT 
TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC 
ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC 
TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC 
GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA 
CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG 
GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT 
GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG 
GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC 
ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC 
AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG 
ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC 
AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA 
GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC 
CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA 
GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC 
AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC 
TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG 
GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT 
ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT 
TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT 
CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG 
GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC 
AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG 
CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275959.1
CDS	131..4972
Translation	MICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_022420251.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420251.1

ORF Insert Sequence:

ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC 
GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT 
TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC 
ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC 
TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC 
GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA 
CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG 
GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT 
GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG 
GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC 
ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC 
AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG 
ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC 
AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA 
GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC 
CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA 
GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC 
AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC 
TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG 
GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT 
ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT 
TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT 
CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG 
GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC 
AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG 
CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_022420252.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022275960.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe289105
Clone ID Related Accession (Same CDS sequence)	XM_022420252.1
Accession Version	XM_022420252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAACC TGCTGGGCAC CCACCTGGGC CACAGTGCCA TCTACAACAT GTGTCGCATC 
ATGGAGGACA GAGCCTACAT GGAAGATGCC CCACTGCTGA GAGGGGCTGT GTTCTTTGTG 
GGGATGGCCC TTTGGGGAGC ACACCGACTC TACTCTCTCA AGAACTCCCC AACGTCTGTG 
TTGCCGTCGT TTTATGAGGC TATGACCTGT CCGAATGAAG TGGTGTCCTA TGAAATCGTC 
CTCTCTATAA CCAGACTCAT CAAGAAGTAC CGGAGGGAGC TGCAAGCTGT GACCTGGGAC 
ATCCTACTGA ACATCATGGA GCGCCTCCTG CACCAGCTCC AGAGCCTGGA CAGCCCGGAG 
CTCAGAGCCA TCGTGCACGA CCTGCTGACC ACAGTGGAAG AGCTGTGTGA TCAGAATGAG 
TTCCACGGCT CTCAGGAGAG ATACTTTGAG CTGGTTGAGC GATGTGCAGA CCAGAGGCCG 
GAGTCTTCCC TCTTAAACTT AATAACGTAC CGGGCTCAGT CAATCCACCC GGCGAAGGAT 
GGCTGGATTC AGAACCTGCA GTTACTGATG GAGAGGTTCT TCAGGAACGA ATCTCGCAGT 
GCTGTGCGCA TCAAGGTGCT GGACGTCCTG TCCTTCGTGC TGCTCATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATCAG CTCGGTGGTC ATCTCACAGC TCTCCCATGT CCCGGAGGAC 
AAAGACCCCC AAGTCCGGAA ACTGGCCACC CAGTTGCTGG TGGACCTGGC CGAAGGCTGC 
CACACCCACC ACTTCAACAG TCTGTTGGAC ATTGTGGAAA AGGTGATTGC CCGCTCTCTC 
TCTCCACCCC CTGAGCTGGA GGAAAGAGAC GTGGCGGTGT ACTCGGCCTC CTTAGAGGAT 
GTGAAGACTG CTGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTGTA CGCCTTGCCT 
GCCAGCCATG CGATGCGCGT GTACGAGACA CTCGTCAGCC ACATCCAGCT CCACTACAAG 
CATAGCTATA CCCTGCCCAT TGCCAGTAGC ATCCGGCTGC AGGCTTTTGA CTTTCTACTG 
CTGCTGAGGG CCGACTCGCT GCACCGCCTC GGCCTGCCCA GCAAGGACGG GGCCGTGAGG 
TTCAGCCCTT ACTGCGTCTG CGATGCCATG GAGCCAGAGA GAGGCTCAGA GAAGAAGGCC 
AGTGGCCCCC TGTCACCTCC CACGGGGCCT CCTGGTCCTG CGCCCGCAGG CCCCGCCGTG 
CGGCTTGGCT CCCTGCCCTA CTCCCTGCTC TTCCGTGTCC TGCTGCAGTG CCTGAAGCTG 
GAGGTGGACT GGAAGGTACT GAAGCTCGTG CTTAGCAAGC TGCCAGAGTC CCTGCGTTAT 
AAGGTGCTCA TCTTCACCTC GCCCTGCAGC GTGGACCAGC TGTCCTCCGC CCTCTGCTCC 
ATGCTCTCAG GTCCCAGAAC TCTGGAGCGG CTCCGAGGCA CCCCGGAAGG GTTCTCCAGA 
ACCGACCTGC ACCTGGCTGT GGTCCCTGTG CTGACGGCGC TCATCTCTTA CCATAACTAC 
CTGGACAAAA CCAGACAGCG GGAGATGGTG TACTGCCTGG AGCAAGGCCT GATCTACCGC 
TGCGCCAGCC AGTGTGTGGT GGCCCTGGCC ATCTGCAGCG TGGAGATGCC TGACATCATC 
ATCAAGGCGC TTCCCGTCCT GGTCGTGAAG CTCACACACA TCTCGGCCAC GGCCAGTATG 
GCCATTCCAC TCCTCGAGTT CCTGTCAACG CTGGCCAGGT TGCCCCACCT GTACAGGAAC 
TTCGCAGCAG AGCAGTACGC GAGTGTGTTT GCCATCTCCC TGCCCTACAC CAATCCCTCC 
AAGTTCAATC AGTACATCGT GTGCCTGGCC CATCATGTCA TAGCCATGTG GTTCATTAGA 
TGCCGCCTGC CCTTCCGGAA GGATTTTGTT CCTTATATCA CTAAGGGCCT GCGCTCCAAC 
GTCCTCCTGT CTTTTGATGA CACCCCCGAG AAGGACAGTT TCAGAGCACG AAGCACCAGT 
CTCAACGAGA GGCCCAAGAG TTTGAGGATA GCCAGACCCC CCAAACAAGG CTTGAATAAC 
TCTCCACCCG TGAAAGAATT CAAAGAGAGC TCTGCCGCCG AGGCCTTCCG ATGCCGCAGC 
ATCAGTGTGT CTGAACATGT GGTCCGCAGC AGGATCCAGA CGTCCCTCAC AAGCGCCAGC 
TTGGGGTCTG CTGACGAGAA CTCGATGGCC CAGGCTGATG ACAACTTGAA AAATCTCCAC 
CTGGAGCTCA CGGAAACATG TCTGGACATG ATGGCCAGAT ATGTGTTCTC CAACTTCACA 
GCAGTCCCCA AGAGGTCCCC GGTGGGAGAG TTCCTCCTGG CTGGTGGCAG GACCAAAACC 
TGGCTGGTTG GGAACAAGCT TGTCACTGTG ACGACGAGCG TGGGGACGGG GACCCGGTCG 
CTGCTGGGCT TGGACTCAGG AGATCAGCAA GGTGGCCCCG AGTTGAGCTC GGACCCTGGC 
ATGCATGTGA GACAGGCAAA GGAGGCACCT GCCAAGCTGG AGTCGCAGGC TGGGCAGCAG 
GTGTGCCGCG GCGCCCGGGA CCGGGTCCGC TCCATGTCCG GGGGCCACGG TCTTCGTGTT 
GGCGCCCTGG ACACTCCAGC CTCCCACTTG CCGGGTGGCC CCACTTCCCC AGGCACGCAG 
GCAGCGCTGG CCAGCAAGTC TGACAGGGCC TCGGCTGGCA CCCGGTTCCC AGCACAGGAG 
AAGACGAGCC TGGCAGCCTA CGTACCGCTG CTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGGAGGCCTA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCGTCGGACA TCAACAACAT GCCTCTGCAG GAGCTGTCCA ACGCGCTCAT GGCCGCTGAG 
CGCTTCAAGG AGCGTCGTGA CACGGCTCTG TACAAGTCGC TGTCGGTGCC TGCCGCAGGC 
TCGGCCAAGC CCCCTCCGCC CCCGAGATCT AACACGGTGG CCTCTTTCTC CTCCCTGTAC 
CAGTCCAGTT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT 
CTGGAGGAGG GAAATTCAAG CGAGACCAGT CTGCCAGTGG AGCACACGGA GCTGGAGGAC 
TTCGAGGCGA CACTAGGCAC AGACCGGCGC TGTGGCCGCG CGGAAGCTTA CAGCAGGTCG 
TCTTCAAGCT CCAGCCAGGA GGAGAAGTTC CCTGCAGAGG AGCTGGCTGC CGGAGGGATC 
CCCATCGAAC GAGCTGTCTC TTCCGAGGGT GCCCGGCCTT CTGTGGAGCT CTCCTTCCAG 
CCCTCCCAGC CCCTCAGCAA GTCCAGCTCC TCCCCTGAGC TGCAGACCCT GCAGGACATA 
CTCGGGGACC CCGGGGACAA GTCTGATGTT GGCCGGCTCA GCCCTGAGGC CAAGGCCCGG 
TCACAGTCCG GGATTCTGGA CGGGGTGGGT GCCTCCTGGT CAGCCCCAGG TGAAGAGAAC 
CAGGGCCAGG GCCCCGCACA GCCCGAGGGT CCCTTGCCTT CCAGCTGCCC CCGCTCCCCC 
AGTGGCCTGC GGCCCAGAGG CTACACCATC TCTGATTCAG CCCCGTCACG CAGAGGCAAG 
AGGGTAGAGC GGGACGCCTT CAGGAGCAGA GCTGGGGCTT CCAACACTGA GAAGGTGCCA 
GGCATCAATC CCAGCTTTGT GTTCCTGCAG CTCTACCACT CACCCTTCTT TGGTGACGAG 
TCCAACAAGC CCATCCTTCT ACCCAATGAG TCCTTCGAGC GGTCAGTGCA GCTTCTCGAC 
CAGATTCCAT CATACGACAC ACACAAGATT GCCGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AGCAGTGAGC TCGCCATCCT GTCCAATGAG CATGGCTCGT ACAGGTACAC GGAGTTCCTG 
ACAGGCCTGG GCAAGCTCAT TGAGCTCAAA GACTGCCAGC CGGACAAGGT GTACCTGGGG 
GGCCTGGACG TGTGTGGCGA AGATGGCCAG TTCACCTACT GCTGGCATGA CGACATCATG 
CAAGCCGTCT TCCACATTGC TACCCTGATG CCCACCAAGG AAGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAATGACTTT GTGTCCATCG TCTACAACGA CTCTGGTGAG 
GACTTCAAGC TGGGCACCAT CCGAGGCCAG TTCAACTTCG TCCACGTGAT CATCACCCCC 
CTGGACTATG AGTGCAACCT GGTGTCCCTG CAGTGCAGGA AAGACATGGA AGGTCTTGTG 
GATACTAGCG TGGCCAAGAT CGTGTCTGAC CGCAACTTGC CTTTTGTGGC ACGTCAGATG 
GCTTTGCATG CAAATATGGC CTCACAAGTG CATCATAGCC GCTCCAACCC CACCGACATC 
TATCCCTCCA AGTGGATTGC CAGGCTTCGC CACATCAAGC GACTCCGCCA CCGGATCCGC 
GAGGAAGCCC ACTACACCAA CCCCAGCCTG CCACTGATGC AGATGCACCC CCCGGGCCAC 
CCCAAAGCCT CTGCTCAGGC GCCGGCAGAG CCTGTGCCCA CGTATGAGAC AGGCCAGCGG 
AAGCGTCTCA TCTCCTCTGT GGATGACTTC ACGGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022275960.1
CDS	113..4777
Translation	MRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_022420252.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420252.1

ORF Insert Sequence:

ATGCGGAACC TGCTGGGCAC CCACCTGGGC CACAGTGCCA TCTACAACAT GTGTCGCATC 
ATGGAGGACA GAGCCTACAT GGAAGATGCC CCACTGCTGA GAGGGGCTGT GTTCTTTGTG 
GGGATGGCCC TTTGGGGAGC ACACCGACTC TACTCTCTCA AGAACTCCCC AACGTCTGTG 
TTGCCGTCGT TTTATGAGGC TATGACCTGT CCGAATGAAG TGGTGTCCTA TGAAATCGTC 
CTCTCTATAA CCAGACTCAT CAAGAAGTAC CGGAGGGAGC TGCAAGCTGT GACCTGGGAC 
ATCCTACTGA ACATCATGGA GCGCCTCCTG CACCAGCTCC AGAGCCTGGA CAGCCCGGAG 
CTCAGAGCCA TCGTGCACGA CCTGCTGACC ACAGTGGAAG AGCTGTGTGA TCAGAATGAG 
TTCCACGGCT CTCAGGAGAG ATACTTTGAG CTGGTTGAGC GATGTGCAGA CCAGAGGCCG 
GAGTCTTCCC TCTTAAACTT AATAACGTAC CGGGCTCAGT CAATCCACCC GGCGAAGGAT 
GGCTGGATTC AGAACCTGCA GTTACTGATG GAGAGGTTCT TCAGGAACGA ATCTCGCAGT 
GCTGTGCGCA TCAAGGTGCT GGACGTCCTG TCCTTCGTGC TGCTCATCAA CAGGCAGTTC 
TATGAGGAGG AGCTGATCAG CTCGGTGGTC ATCTCACAGC TCTCCCATGT CCCGGAGGAC 
AAAGACCCCC AAGTCCGGAA ACTGGCCACC CAGTTGCTGG TGGACCTGGC CGAAGGCTGC 
CACACCCACC ACTTCAACAG TCTGTTGGAC ATTGTGGAAA AGGTGATTGC CCGCTCTCTC 
TCTCCACCCC CTGAGCTGGA GGAAAGAGAC GTGGCGGTGT ACTCGGCCTC CTTAGAGGAT 
GTGAAGACTG CTGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTGTA CGCCTTGCCT 
GCCAGCCATG CGATGCGCGT GTACGAGACA CTCGTCAGCC ACATCCAGCT CCACTACAAG 
CATAGCTATA CCCTGCCCAT TGCCAGTAGC ATCCGGCTGC AGGCTTTTGA CTTTCTACTG 
CTGCTGAGGG CCGACTCGCT GCACCGCCTC GGCCTGCCCA GCAAGGACGG GGCCGTGAGG 
TTCAGCCCTT ACTGCGTCTG CGATGCCATG GAGCCAGAGA GAGGCTCAGA GAAGAAGGCC 
AGTGGCCCCC TGTCACCTCC CACGGGGCCT CCTGGTCCTG CGCCCGCAGG CCCCGCCGTG 
CGGCTTGGCT CCCTGCCCTA CTCCCTGCTC TTCCGTGTCC TGCTGCAGTG CCTGAAGCTG 
GAGGTGGACT GGAAGGTACT GAAGCTCGTG CTTAGCAAGC TGCCAGAGTC CCTGCGTTAT 
AAGGTGCTCA TCTTCACCTC GCCCTGCAGC GTGGACCAGC TGTCCTCCGC CCTCTGCTCC 
ATGCTCTCAG GTCCCAGAAC TCTGGAGCGG CTCCGAGGCA CCCCGGAAGG GTTCTCCAGA 
ACCGACCTGC ACCTGGCTGT GGTCCCTGTG CTGACGGCGC TCATCTCTTA CCATAACTAC 
CTGGACAAAA CCAGACAGCG GGAGATGGTG TACTGCCTGG AGCAAGGCCT GATCTACCGC 
TGCGCCAGCC AGTGTGTGGT GGCCCTGGCC ATCTGCAGCG TGGAGATGCC TGACATCATC 
ATCAAGGCGC TTCCCGTCCT GGTCGTGAAG CTCACACACA TCTCGGCCAC GGCCAGTATG 
GCCATTCCAC TCCTCGAGTT CCTGTCAACG CTGGCCAGGT TGCCCCACCT GTACAGGAAC 
TTCGCAGCAG AGCAGTACGC GAGTGTGTTT GCCATCTCCC TGCCCTACAC CAATCCCTCC 
AAGTTCAATC AGTACATCGT GTGCCTGGCC CATCATGTCA TAGCCATGTG GTTCATTAGA 
TGCCGCCTGC CCTTCCGGAA GGATTTTGTT CCTTATATCA CTAAGGGCCT GCGCTCCAAC 
GTCCTCCTGT CTTTTGATGA CACCCCCGAG AAGGACAGTT TCAGAGCACG AAGCACCAGT 
CTCAACGAGA GGCCCAAGAG TTTGAGGATA GCCAGACCCC CCAAACAAGG CTTGAATAAC 
TCTCCACCCG TGAAAGAATT CAAAGAGAGC TCTGCCGCCG AGGCCTTCCG ATGCCGCAGC 
ATCAGTGTGT CTGAACATGT GGTCCGCAGC AGGATCCAGA CGTCCCTCAC AAGCGCCAGC 
TTGGGGTCTG CTGACGAGAA CTCGATGGCC CAGGCTGATG ACAACTTGAA AAATCTCCAC 
CTGGAGCTCA CGGAAACATG TCTGGACATG ATGGCCAGAT ATGTGTTCTC CAACTTCACA 
GCAGTCCCCA AGAGGTCCCC GGTGGGAGAG TTCCTCCTGG CTGGTGGCAG GACCAAAACC 
TGGCTGGTTG GGAACAAGCT TGTCACTGTG ACGACGAGCG TGGGGACGGG GACCCGGTCG 
CTGCTGGGCT TGGACTCAGG AGATCAGCAA GGTGGCCCCG AGTTGAGCTC GGACCCTGGC 
ATGCATGTGA GACAGGCAAA GGAGGCACCT GCCAAGCTGG AGTCGCAGGC TGGGCAGCAG 
GTGTGCCGCG GCGCCCGGGA CCGGGTCCGC TCCATGTCCG GGGGCCACGG TCTTCGTGTT 
GGCGCCCTGG ACACTCCAGC CTCCCACTTG CCGGGTGGCC CCACTTCCCC AGGCACGCAG 
GCAGCGCTGG CCAGCAAGTC TGACAGGGCC TCGGCTGGCA CCCGGTTCCC AGCACAGGAG 
AAGACGAGCC TGGCAGCCTA CGTACCGCTG CTGACCCAGG GCTGGGCGGA GATCTTGGTC 
CGGAGGCCTA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCGTCGGACA TCAACAACAT GCCTCTGCAG GAGCTGTCCA ACGCGCTCAT GGCCGCTGAG 
CGCTTCAAGG AGCGTCGTGA CACGGCTCTG TACAAGTCGC TGTCGGTGCC TGCCGCAGGC 
TCGGCCAAGC CCCCTCCGCC CCCGAGATCT AACACGGTGG CCTCTTTCTC CTCCCTGTAC 
CAGTCCAGTT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT 
CTGGAGGAGG GAAATTCAAG CGAGACCAGT CTGCCAGTGG AGCACACGGA GCTGGAGGAC 
TTCGAGGCGA CACTAGGCAC AGACCGGCGC TGTGGCCGCG CGGAAGCTTA CAGCAGGTCG 
TCTTCAAGCT CCAGCCAGGA GGAGAAGTTC CCTGCAGAGG AGCTGGCTGC CGGAGGGATC 
CCCATCGAAC GAGCTGTCTC TTCCGAGGGT GCCCGGCCTT CTGTGGAGCT CTCCTTCCAG 
CCCTCCCAGC CCCTCAGCAA GTCCAGCTCC TCCCCTGAGC TGCAGACCCT GCAGGACATA 
CTCGGGGACC CCGGGGACAA GTCTGATGTT GGCCGGCTCA GCCCTGAGGC CAAGGCCCGG 
TCACAGTCCG GGATTCTGGA CGGGGTGGGT GCCTCCTGGT CAGCCCCAGG TGAAGAGAAC 
CAGGGCCAGG GCCCCGCACA GCCCGAGGGT CCCTTGCCTT CCAGCTGCCC CCGCTCCCCC 
AGTGGCCTGC GGCCCAGAGG CTACACCATC TCTGATTCAG CCCCGTCACG CAGAGGCAAG 
AGGGTAGAGC GGGACGCCTT CAGGAGCAGA GCTGGGGCTT CCAACACTGA GAAGGTGCCA 
GGCATCAATC CCAGCTTTGT GTTCCTGCAG CTCTACCACT CACCCTTCTT TGGTGACGAG 
TCCAACAAGC CCATCCTTCT ACCCAATGAG TCCTTCGAGC GGTCAGTGCA GCTTCTCGAC 
CAGATTCCAT CATACGACAC ACACAAGATT GCCGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AGCAGTGAGC TCGCCATCCT GTCCAATGAG CATGGCTCGT ACAGGTACAC GGAGTTCCTG 
ACAGGCCTGG GCAAGCTCAT TGAGCTCAAA GACTGCCAGC CGGACAAGGT GTACCTGGGG 
GGCCTGGACG TGTGTGGCGA AGATGGCCAG TTCACCTACT GCTGGCATGA CGACATCATG 
CAAGCCGTCT TCCACATTGC TACCCTGATG CCCACCAAGG AAGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAATGACTTT GTGTCCATCG TCTACAACGA CTCTGGTGAG 
GACTTCAAGC TGGGCACCAT CCGAGGCCAG TTCAACTTCG TCCACGTGAT CATCACCCCC 
CTGGACTATG AGTGCAACCT GGTGTCCCTG CAGTGCAGGA AAGACATGGA AGGTCTTGTG 
GATACTAGCG TGGCCAAGAT CGTGTCTGAC CGCAACTTGC CTTTTGTGGC ACGTCAGATG 
GCTTTGCATG CAAATATGGC CTCACAAGTG CATCATAGCC GCTCCAACCC CACCGACATC 
TATCCCTCCA AGTGGATTGC CAGGCTTCGC CACATCAAGC GACTCCGCCA CCGGATCCGC 
GAGGAAGCCC ACTACACCAA CCCCAGCCTG CCACTGATGC AGATGCACCC CCCGGGCCAC 
CCCAAAGCCT CTGCTCAGGC GCCGGCAGAG CCTGTGCCCA CGTATGAGAC AGGCCAGCGG 
AAGCGTCTCA TCTCCTCTGT GGATGACTTC ACGGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_014114692.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970167.1 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe37159
Clone ID Related Accession (Same CDS sequence)	XM_014114692.1 , XM_014114692.2
Accession Version	XM_014114692.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tuberin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC 
GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA 
ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG 
TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC 
TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG 
GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT 
CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC 
AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA 
GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC 
AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT 
CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT 
CCGCCCCCGA GATCTAACAC GGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG 
ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC 
CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG 
AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC 
GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC 
AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT 
GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG 
GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC 
GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC 
ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG 
AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC 
CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC 
AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG 
CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT 
GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC 
CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA 
GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA 
CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC 
AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG 
GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT 
GACTTCACGG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970167.1
CDS	282..5522
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_014114692.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114692.1

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC 
GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA 
ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG 
TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC 
TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG 
GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT 
CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC 
AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA 
GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC 
AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT 
CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT 
CCGCCCCCGA GATCTAACAC GGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG 
ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC 
CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG 
AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC 
GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC 
AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT 
GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG 
GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC 
GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC 
ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG 
AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC 
CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC 
AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG 
CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT 
GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC 
CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA 
GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA 
CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC 
AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG 
GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT 
GACTTCACGG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_014114692.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970167.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe37159
Clone ID Related Accession (Same CDS sequence)	XM_014114692.1 , XM_014114692.2
Accession Version	XM_014114692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC 
GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA 
ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG 
TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC 
TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG 
GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT 
CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC 
AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA 
GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC 
AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT 
CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT 
CCGCCCCCGA GATCTAACAC GGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG 
ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC 
CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG 
AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC 
GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC 
AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT 
GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG 
GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC 
GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC 
ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG 
AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC 
CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC 
AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG 
CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT 
GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC 
CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA 
GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA 
CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC 
AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG 
GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT 
GACTTCACGG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970167.1
CDS	289..5529
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_014114692.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114692.2

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC 
GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA 
ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG 
TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC 
TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG 
GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT 
CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC 
AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA 
GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC 
AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT 
CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT 
CCGCCCCCGA GATCTAACAC GGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG 
ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC 
CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG 
AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC 
GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC 
AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT 
GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG 
GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC 
GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC 
ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG 
AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC 
CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC 
AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC 
AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG 
CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT 
GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC 
CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA 
GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA 
CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG 
CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC 
AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG 
GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT 
GACTTCACGG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621715.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621772.1 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe08328
Clone ID Related Accession (Same CDS sequence)	XM_005621715.2 , XM_005621715.3
Accession Version	XM_005621715.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tuberin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC 
GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT 
TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC 
ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC 
TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC 
GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA 
CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG 
GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT 
GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG 
GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC 
ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC 
AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG 
ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC 
AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA 
GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC 
CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA 
GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC 
AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC 
TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG 
GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT 
ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT 
TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT 
CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG 
GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC 
AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG 
CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621772.1
CDS	283..5724
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621715.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621715.2

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC 
GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT 
TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC 
ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC 
TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC 
GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA 
CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG 
GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT 
GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG 
GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC 
ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC 
AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG 
ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC 
AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA 
GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC 
CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA 
GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC 
AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC 
TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG 
GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT 
ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT 
TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT 
CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG 
GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC 
AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG 
CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621715.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621772.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe08328
Clone ID Related Accession (Same CDS sequence)	XM_005621715.2 , XM_005621715.3
Accession Version	XM_005621715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC 
GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT 
TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC 
ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC 
TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC 
GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA 
CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG 
GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT 
GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG 
GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC 
ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC 
AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG 
ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC 
AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA 
GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC 
CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA 
GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC 
AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC 
TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG 
GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT 
ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT 
TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT 
CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG 
GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC 
AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG 
CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621772.1
CDS	290..5731
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621715.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621715.3

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG 
GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC 
GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT 
TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC 
ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC 
TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC 
GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA 
CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG 
GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT 
GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG 
GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC 
ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC 
AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG 
ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC 
AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA 
GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC 
CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA 
GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC 
AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC 
TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG 
GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT 
ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT 
TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT 
CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG 
GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC 
AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG 
CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621716.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621773.1 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe08329
Clone ID Related Accession (Same CDS sequence)	XM_005621716.2 , XM_005621716.3
Accession Version	XM_005621716.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tuberin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACGTCCC TCACAAGCGC CAGCTTGGGG 
TCTGCTGACG AGAACTCGAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCACCTGGAG 
CTCACGGAAA CATGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACAGCAGTC 
CCCAAGAGGT CCCCGGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC TGTGACGACG AGCGTGGGGA CGGGGACCCG GTCGCTGCTG 
GGCTTGGACT CAGGAGATCA GCAAGGTGGC CCCGAGTTGA GCTCGGACCC TGGCATGCAT 
GTGAGACAGG CAAAGGAGGC ACCTGCCAAG CTGGAGTCGC AGGCTGGGCA GCAGGTGTGC 
CGCGGCGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ACGGTCTTCG TGTTGGCGCC 
CTGGACACTC CAGCCTCCCA CTTGCCGGGT GGCCCCACTT CCCCAGGCAC GCAGGCAGCG 
CTGGCCAGCA AGTCTGACAG GGCCTCGGCT GGCACCCGGT TCCCAGCACA GGAGAAGACG 
AGCCTGGCAG CCTACGTACC GCTGCTGACC CAGGGCTGGG CGGAGATCTT GGTCCGGAGG 
CCTACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC CTTCTCGTCG 
GACATCAACA ACATGCCTCT GCAGGAGCTG TCCAACGCGC TCATGGCCGC TGAGCGCTTC 
AAGGAGCGTC GTGACACGGC TCTGTACAAG TCGCTGTCGG TGCCTGCCGC AGGCTCGGCC 
AAGCCCCCTC CGCCCCCGAG ATCTAACACG GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGTTGCCAAG GAAAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCGGT GGTTCTGGAG 
GAGGGAAATT CAAGCGAGAC CAGTCTGCCA GTGGAGCACA CGGAGCTGGA GGACTTCGAG 
GCGACACTAG GCACAGACCG GCGCTGTGGC CGCGCGGAAG CTTACAGCAG GTCGTCTTCA 
AGCTCCAGCC AGGAGGAGAA GTTCCCTGCA GAGGAGCTGG CTGCCGGAGG GATCCCCATC 
GAACGAGCTG TCTCTTCCGA GGGTGCCCGG CCTTCTGTGG AGCTCTCCTT CCAGCCCTCC 
CAGCCCCTCA GCAAGTCCAG CTCCTCCCCT GAGCTGCAGA CCCTGCAGGA CATACTCGGG 
GACCCCGGGG ACAAGTCTGA TGTTGGCCGG CTCAGCCCTG AGGCCAAGGC CCGGTCACAG 
TCCGGGATTC TGGACGGGGT GGGTGCCTCC TGGTCAGCCC CAGGTGAAGA GAACCAGGGC 
CAGGGCCCCG CACAGCCCGA GGGTCCCTTG CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCCA GAGGCTACAC CATCTCTGAT TCAGCCCCGT CACGCAGAGG CAAGAGGGTA 
GAGCGGGACG CCTTCAGGAG CAGAGCTGGG GCTTCCAACA CTGAGAAGGT GCCAGGCATC 
AATCCCAGCT TTGTGTTCCT GCAGCTCTAC CACTCACCCT TCTTTGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTACCCAA TGAGTCCTTC GAGCGGTCAG TGCAGCTTCT CGACCAGATT 
CCATCATACG ACACACACAA GATTGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTCGCCA TCCTGTCCAA TGAGCATGGC TCGTACAGGT ACACGGAGTT CCTGACAGGC 
CTGGGCAAGC TCATTGAGCT CAAAGACTGC CAGCCGGACA AGGTGTACCT GGGGGGCCTG 
GACGTGTGTG GCGAAGATGG CCAGTTCACC TACTGCTGGC ATGACGACAT CATGCAAGCC 
GTCTTCCACA TTGCTACCCT GATGCCCACC AAGGAAGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA CTTTGTGTCC ATCGTCTACA ACGACTCTGG TGAGGACTTC 
AAGCTGGGCA CCATCCGAGG CCAGTTCAAC TTCGTCCACG TGATCATCAC CCCCCTGGAC 
TATGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAAGGTCT TGTGGATACT 
AGCGTGGCCA AGATCGTGTC TGACCGCAAC TTGCCTTTTG TGGCACGTCA GATGGCTTTG 
CATGCAAATA TGGCCTCACA AGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTATCCC 
TCCAAGTGGA TTGCCAGGCT TCGCCACATC AAGCGACTCC GCCACCGGAT CCGCGAGGAA 
GCCCACTACA CCAACCCCAG CCTGCCACTG ATGCAGATGC ACCCCCCGGG CCACCCCAAA 
GCCTCTGCTC AGGCGCCGGC AGAGCCTGTG CCCACGTATG AGACAGGCCA GCGGAAGCGT 
CTCATCTCCT CTGTGGATGA CTTCACGGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621773.1
CDS	283..5721
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621716.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621716.2

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACGTCCC TCACAAGCGC CAGCTTGGGG 
TCTGCTGACG AGAACTCGAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCACCTGGAG 
CTCACGGAAA CATGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACAGCAGTC 
CCCAAGAGGT CCCCGGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC TGTGACGACG AGCGTGGGGA CGGGGACCCG GTCGCTGCTG 
GGCTTGGACT CAGGAGATCA GCAAGGTGGC CCCGAGTTGA GCTCGGACCC TGGCATGCAT 
GTGAGACAGG CAAAGGAGGC ACCTGCCAAG CTGGAGTCGC AGGCTGGGCA GCAGGTGTGC 
CGCGGCGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ACGGTCTTCG TGTTGGCGCC 
CTGGACACTC CAGCCTCCCA CTTGCCGGGT GGCCCCACTT CCCCAGGCAC GCAGGCAGCG 
CTGGCCAGCA AGTCTGACAG GGCCTCGGCT GGCACCCGGT TCCCAGCACA GGAGAAGACG 
AGCCTGGCAG CCTACGTACC GCTGCTGACC CAGGGCTGGG CGGAGATCTT GGTCCGGAGG 
CCTACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC CTTCTCGTCG 
GACATCAACA ACATGCCTCT GCAGGAGCTG TCCAACGCGC TCATGGCCGC TGAGCGCTTC 
AAGGAGCGTC GTGACACGGC TCTGTACAAG TCGCTGTCGG TGCCTGCCGC AGGCTCGGCC 
AAGCCCCCTC CGCCCCCGAG ATCTAACACG GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGTTGCCAAG GAAAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCGGT GGTTCTGGAG 
GAGGGAAATT CAAGCGAGAC CAGTCTGCCA GTGGAGCACA CGGAGCTGGA GGACTTCGAG 
GCGACACTAG GCACAGACCG GCGCTGTGGC CGCGCGGAAG CTTACAGCAG GTCGTCTTCA 
AGCTCCAGCC AGGAGGAGAA GTTCCCTGCA GAGGAGCTGG CTGCCGGAGG GATCCCCATC 
GAACGAGCTG TCTCTTCCGA GGGTGCCCGG CCTTCTGTGG AGCTCTCCTT CCAGCCCTCC 
CAGCCCCTCA GCAAGTCCAG CTCCTCCCCT GAGCTGCAGA CCCTGCAGGA CATACTCGGG 
GACCCCGGGG ACAAGTCTGA TGTTGGCCGG CTCAGCCCTG AGGCCAAGGC CCGGTCACAG 
TCCGGGATTC TGGACGGGGT GGGTGCCTCC TGGTCAGCCC CAGGTGAAGA GAACCAGGGC 
CAGGGCCCCG CACAGCCCGA GGGTCCCTTG CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCCA GAGGCTACAC CATCTCTGAT TCAGCCCCGT CACGCAGAGG CAAGAGGGTA 
GAGCGGGACG CCTTCAGGAG CAGAGCTGGG GCTTCCAACA CTGAGAAGGT GCCAGGCATC 
AATCCCAGCT TTGTGTTCCT GCAGCTCTAC CACTCACCCT TCTTTGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTACCCAA TGAGTCCTTC GAGCGGTCAG TGCAGCTTCT CGACCAGATT 
CCATCATACG ACACACACAA GATTGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTCGCCA TCCTGTCCAA TGAGCATGGC TCGTACAGGT ACACGGAGTT CCTGACAGGC 
CTGGGCAAGC TCATTGAGCT CAAAGACTGC CAGCCGGACA AGGTGTACCT GGGGGGCCTG 
GACGTGTGTG GCGAAGATGG CCAGTTCACC TACTGCTGGC ATGACGACAT CATGCAAGCC 
GTCTTCCACA TTGCTACCCT GATGCCCACC AAGGAAGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA CTTTGTGTCC ATCGTCTACA ACGACTCTGG TGAGGACTTC 
AAGCTGGGCA CCATCCGAGG CCAGTTCAAC TTCGTCCACG TGATCATCAC CCCCCTGGAC 
TATGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAAGGTCT TGTGGATACT 
AGCGTGGCCA AGATCGTGTC TGACCGCAAC TTGCCTTTTG TGGCACGTCA GATGGCTTTG 
CATGCAAATA TGGCCTCACA AGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTATCCC 
TCCAAGTGGA TTGCCAGGCT TCGCCACATC AAGCGACTCC GCCACCGGAT CCGCGAGGAA 
GCCCACTACA CCAACCCCAG CCTGCCACTG ATGCAGATGC ACCCCCCGGG CCACCCCAAA 
GCCTCTGCTC AGGCGCCGGC AGAGCCTGTG CCCACGTATG AGACAGGCCA GCGGAAGCGT 
CTCATCTCCT CTGTGGATGA CTTCACGGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621716.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621773.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe08329
Clone ID Related Accession (Same CDS sequence)	XM_005621716.2 , XM_005621716.3
Accession Version	XM_005621716.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACGTCCC TCACAAGCGC CAGCTTGGGG 
TCTGCTGACG AGAACTCGAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCACCTGGAG 
CTCACGGAAA CATGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACAGCAGTC 
CCCAAGAGGT CCCCGGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC TGTGACGACG AGCGTGGGGA CGGGGACCCG GTCGCTGCTG 
GGCTTGGACT CAGGAGATCA GCAAGGTGGC CCCGAGTTGA GCTCGGACCC TGGCATGCAT 
GTGAGACAGG CAAAGGAGGC ACCTGCCAAG CTGGAGTCGC AGGCTGGGCA GCAGGTGTGC 
CGCGGCGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ACGGTCTTCG TGTTGGCGCC 
CTGGACACTC CAGCCTCCCA CTTGCCGGGT GGCCCCACTT CCCCAGGCAC GCAGGCAGCG 
CTGGCCAGCA AGTCTGACAG GGCCTCGGCT GGCACCCGGT TCCCAGCACA GGAGAAGACG 
AGCCTGGCAG CCTACGTACC GCTGCTGACC CAGGGCTGGG CGGAGATCTT GGTCCGGAGG 
CCTACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC CTTCTCGTCG 
GACATCAACA ACATGCCTCT GCAGGAGCTG TCCAACGCGC TCATGGCCGC TGAGCGCTTC 
AAGGAGCGTC GTGACACGGC TCTGTACAAG TCGCTGTCGG TGCCTGCCGC AGGCTCGGCC 
AAGCCCCCTC CGCCCCCGAG ATCTAACACG GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGTTGCCAAG GAAAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCGGT GGTTCTGGAG 
GAGGGAAATT CAAGCGAGAC CAGTCTGCCA GTGGAGCACA CGGAGCTGGA GGACTTCGAG 
GCGACACTAG GCACAGACCG GCGCTGTGGC CGCGCGGAAG CTTACAGCAG GTCGTCTTCA 
AGCTCCAGCC AGGAGGAGAA GTTCCCTGCA GAGGAGCTGG CTGCCGGAGG GATCCCCATC 
GAACGAGCTG TCTCTTCCGA GGGTGCCCGG CCTTCTGTGG AGCTCTCCTT CCAGCCCTCC 
CAGCCCCTCA GCAAGTCCAG CTCCTCCCCT GAGCTGCAGA CCCTGCAGGA CATACTCGGG 
GACCCCGGGG ACAAGTCTGA TGTTGGCCGG CTCAGCCCTG AGGCCAAGGC CCGGTCACAG 
TCCGGGATTC TGGACGGGGT GGGTGCCTCC TGGTCAGCCC CAGGTGAAGA GAACCAGGGC 
CAGGGCCCCG CACAGCCCGA GGGTCCCTTG CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCCA GAGGCTACAC CATCTCTGAT TCAGCCCCGT CACGCAGAGG CAAGAGGGTA 
GAGCGGGACG CCTTCAGGAG CAGAGCTGGG GCTTCCAACA CTGAGAAGGT GCCAGGCATC 
AATCCCAGCT TTGTGTTCCT GCAGCTCTAC CACTCACCCT TCTTTGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTACCCAA TGAGTCCTTC GAGCGGTCAG TGCAGCTTCT CGACCAGATT 
CCATCATACG ACACACACAA GATTGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTCGCCA TCCTGTCCAA TGAGCATGGC TCGTACAGGT ACACGGAGTT CCTGACAGGC 
CTGGGCAAGC TCATTGAGCT CAAAGACTGC CAGCCGGACA AGGTGTACCT GGGGGGCCTG 
GACGTGTGTG GCGAAGATGG CCAGTTCACC TACTGCTGGC ATGACGACAT CATGCAAGCC 
GTCTTCCACA TTGCTACCCT GATGCCCACC AAGGAAGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA CTTTGTGTCC ATCGTCTACA ACGACTCTGG TGAGGACTTC 
AAGCTGGGCA CCATCCGAGG CCAGTTCAAC TTCGTCCACG TGATCATCAC CCCCCTGGAC 
TATGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAAGGTCT TGTGGATACT 
AGCGTGGCCA AGATCGTGTC TGACCGCAAC TTGCCTTTTG TGGCACGTCA GATGGCTTTG 
CATGCAAATA TGGCCTCACA AGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTATCCC 
TCCAAGTGGA TTGCCAGGCT TCGCCACATC AAGCGACTCC GCCACCGGAT CCGCGAGGAA 
GCCCACTACA CCAACCCCAG CCTGCCACTG ATGCAGATGC ACCCCCCGGG CCACCCCAAA 
GCCTCTGCTC AGGCGCCGGC AGAGCCTGTG CCCACGTATG AGACAGGCCA GCGGAAGCGT 
CTCATCTCCT CTGTGGATGA CTTCACGGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621773.1
CDS	290..5728
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621716.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621716.3

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACGTCCC TCACAAGCGC CAGCTTGGGG 
TCTGCTGACG AGAACTCGAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCACCTGGAG 
CTCACGGAAA CATGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACAGCAGTC 
CCCAAGAGGT CCCCGGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC TGTGACGACG AGCGTGGGGA CGGGGACCCG GTCGCTGCTG 
GGCTTGGACT CAGGAGATCA GCAAGGTGGC CCCGAGTTGA GCTCGGACCC TGGCATGCAT 
GTGAGACAGG CAAAGGAGGC ACCTGCCAAG CTGGAGTCGC AGGCTGGGCA GCAGGTGTGC 
CGCGGCGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ACGGTCTTCG TGTTGGCGCC 
CTGGACACTC CAGCCTCCCA CTTGCCGGGT GGCCCCACTT CCCCAGGCAC GCAGGCAGCG 
CTGGCCAGCA AGTCTGACAG GGCCTCGGCT GGCACCCGGT TCCCAGCACA GGAGAAGACG 
AGCCTGGCAG CCTACGTACC GCTGCTGACC CAGGGCTGGG CGGAGATCTT GGTCCGGAGG 
CCTACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC CTTCTCGTCG 
GACATCAACA ACATGCCTCT GCAGGAGCTG TCCAACGCGC TCATGGCCGC TGAGCGCTTC 
AAGGAGCGTC GTGACACGGC TCTGTACAAG TCGCTGTCGG TGCCTGCCGC AGGCTCGGCC 
AAGCCCCCTC CGCCCCCGAG ATCTAACACG GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGTTGCCAAG GAAAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCGGT GGTTCTGGAG 
GAGGGAAATT CAAGCGAGAC CAGTCTGCCA GTGGAGCACA CGGAGCTGGA GGACTTCGAG 
GCGACACTAG GCACAGACCG GCGCTGTGGC CGCGCGGAAG CTTACAGCAG GTCGTCTTCA 
AGCTCCAGCC AGGAGGAGAA GTTCCCTGCA GAGGAGCTGG CTGCCGGAGG GATCCCCATC 
GAACGAGCTG TCTCTTCCGA GGGTGCCCGG CCTTCTGTGG AGCTCTCCTT CCAGCCCTCC 
CAGCCCCTCA GCAAGTCCAG CTCCTCCCCT GAGCTGCAGA CCCTGCAGGA CATACTCGGG 
GACCCCGGGG ACAAGTCTGA TGTTGGCCGG CTCAGCCCTG AGGCCAAGGC CCGGTCACAG 
TCCGGGATTC TGGACGGGGT GGGTGCCTCC TGGTCAGCCC CAGGTGAAGA GAACCAGGGC 
CAGGGCCCCG CACAGCCCGA GGGTCCCTTG CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC 
CTGCGGCCCA GAGGCTACAC CATCTCTGAT TCAGCCCCGT CACGCAGAGG CAAGAGGGTA 
GAGCGGGACG CCTTCAGGAG CAGAGCTGGG GCTTCCAACA CTGAGAAGGT GCCAGGCATC 
AATCCCAGCT TTGTGTTCCT GCAGCTCTAC CACTCACCCT TCTTTGGTGA CGAGTCCAAC 
AAGCCCATCC TTCTACCCAA TGAGTCCTTC GAGCGGTCAG TGCAGCTTCT CGACCAGATT 
CCATCATACG ACACACACAA GATTGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAGCAGT 
GAGCTCGCCA TCCTGTCCAA TGAGCATGGC TCGTACAGGT ACACGGAGTT CCTGACAGGC 
CTGGGCAAGC TCATTGAGCT CAAAGACTGC CAGCCGGACA AGGTGTACCT GGGGGGCCTG 
GACGTGTGTG GCGAAGATGG CCAGTTCACC TACTGCTGGC ATGACGACAT CATGCAAGCC 
GTCTTCCACA TTGCTACCCT GATGCCCACC AAGGAAGTGG ACAAGCACCG CTGTGACAAG 
AAGCGCCACC TGGGCAATGA CTTTGTGTCC ATCGTCTACA ACGACTCTGG TGAGGACTTC 
AAGCTGGGCA CCATCCGAGG CCAGTTCAAC TTCGTCCACG TGATCATCAC CCCCCTGGAC 
TATGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAAGGTCT TGTGGATACT 
AGCGTGGCCA AGATCGTGTC TGACCGCAAC TTGCCTTTTG TGGCACGTCA GATGGCTTTG 
CATGCAAATA TGGCCTCACA AGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTATCCC 
TCCAAGTGGA TTGCCAGGCT TCGCCACATC AAGCGACTCC GCCACCGGAT CCGCGAGGAA 
GCCCACTACA CCAACCCCAG CCTGCCACTG ATGCAGATGC ACCCCCCGGG CCACCCCAAA 
GCCTCTGCTC AGGCGCCGGC AGAGCCTGTG CCCACGTATG AGACAGGCCA GCGGAAGCGT 
CTCATCTCCT CTGTGGATGA CTTCACGGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621717.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621774.1 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe08331
Clone ID Related Accession (Same CDS sequence)	XM_005621717.2 , XM_005621717.3
Accession Version	XM_005621717.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tuberin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGACTCTG CGGTGGTTCT GGAGGAGGGA 
AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA 
CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC 
AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA 
GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC 
CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC 
GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG 
ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC 
CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG 
CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG 
GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC 
AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC 
ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA 
TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC 
GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC 
AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG 
TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC 
CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG 
GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG 
TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG 
GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA 
AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG 
TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC 
TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT 
GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC 
TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621774.1
CDS	283..5655
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621717.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621717.2

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGACTCTG CGGTGGTTCT GGAGGAGGGA 
AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA 
CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC 
AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA 
GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC 
CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC 
GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG 
ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC 
CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG 
CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG 
GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC 
AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC 
ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA 
TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC 
GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC 
AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG 
TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC 
CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG 
GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG 
TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG 
GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA 
AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG 
TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC 
TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT 
GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC 
TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621717.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621774.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe08331
Clone ID Related Accession (Same CDS sequence)	XM_005621717.2 , XM_005621717.3
Accession Version	XM_005621717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621717.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGACTCTG CGGTGGTTCT GGAGGAGGGA 
AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA 
CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC 
AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA 
GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC 
CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC 
GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG 
ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC 
CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG 
CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG 
GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC 
AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC 
ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA 
TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC 
GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC 
AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG 
TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC 
CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG 
GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG 
TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG 
GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA 
AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG 
TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC 
TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT 
GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC 
TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621774.1
CDS	289..5661
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621717.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621717.3

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG 
GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG 
GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA 
GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG 
CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG 
CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG 
TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC 
GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA 
GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG 
ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG 
AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG 
TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC 
TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG 
GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGACTCTG CGGTGGTTCT GGAGGAGGGA 
AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA 
CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC 
AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA 
GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC 
CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC 
GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG 
ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC 
CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG 
CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG 
GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC 
AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC 
ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA 
TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC 
GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC 
AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG 
TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC 
CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG 
GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG 
TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG 
GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA 
AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG 
TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC 
TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT 
GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC 
TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621718.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621775.1 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe08333
Clone ID Related Accession (Same CDS sequence)	XM_005621718.2 , XM_005621718.3
Accession Version	XM_005621718.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tuberin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621718.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT 
GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG 
GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG 
AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG 
GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA 
CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC 
ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC 
AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG 
GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC 
AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG 
CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC 
CCTCCGCCCC CGAGATCTAA CACGGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGTTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA 
CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC 
AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA 
GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC 
CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC 
GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG 
ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC 
CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG 
CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG 
GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC 
AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC 
ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA 
TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC 
GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC 
AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG 
TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC 
CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG 
GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG 
TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG 
GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA 
AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG 
TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC 
TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT 
GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC 
TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621775.1
CDS	283..5595
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621718.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621718.2

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT 
GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG 
GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG 
AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG 
GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA 
CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC 
ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC 
AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG 
GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC 
AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG 
CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC 
CCTCCGCCCC CGAGATCTAA CACGGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGTTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA 
CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC 
AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA 
GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC 
CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC 
GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG 
ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC 
CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG 
CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG 
GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC 
AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC 
ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA 
TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC 
GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC 
AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG 
TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC 
CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG 
GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG 
TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG 
GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA 
AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG 
TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC 
TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT 
GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC 
TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621718.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621775.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe08333
Clone ID Related Accession (Same CDS sequence)	XM_005621718.2 , XM_005621718.3
Accession Version	XM_005621718.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621718.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT 
GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG 
GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG 
AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG 
GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA 
CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC 
ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC 
AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG 
GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC 
AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG 
CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC 
CCTCCGCCCC CGAGATCTAA CACGGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGTTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA 
CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC 
AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA 
GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC 
CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC 
GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG 
ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC 
CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG 
CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG 
GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC 
AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC 
ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA 
TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC 
GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC 
AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG 
TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC 
CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG 
GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG 
TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG 
GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA 
AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG 
TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC 
TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT 
GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC 
TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621775.1
CDS	290..5602
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621718.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621718.3

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT 
GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG 
GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG 
AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG 
GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA 
CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC 
ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC 
AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG 
GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC 
AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG 
CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC 
CCTCCGCCCC CGAGATCTAA CACGGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGTTGC 
CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA 
AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA 
CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC 
AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA 
GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC 
CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC 
GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG 
ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC 
CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG 
CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG 
GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC 
AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC 
ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA 
TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC 
GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC 
AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG 
TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC 
CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG 
GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG 
TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG 
GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA 
AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG 
TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC 
TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT 
GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC 
TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621719.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621776.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe08334
Clone ID Related Accession (Same CDS sequence)	XM_005621719.3 , XM_005621719.2
Accession Version	XM_005621719.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621719.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC 
GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA 
ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG 
TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC 
TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG 
GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT 
CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC 
AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA 
GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC 
AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT 
CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT 
CCGCCCCCGA GATCTAACAC GGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGTTGCCAA 
GGAAAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCGG TGGTTCTGGA GGAGGGAAAT 
TCAAGCGAGA CCAGTCTGCC AGTGGAGCAC ACGGAGCTGG AGGACTTCGA GGCGACACTA 
GGCACAGACC GGCGCTGTGG CCGCGCGGAA GCTTACAGCA GGTCGTCTTC AAGCTCCAGC 
CAGGAGGAGA AGTTCCCTGC AGAGGAGCTG GCTGCCGGAG GGATCCCCAT CGAACGAGCT 
GTCTCTTCCG AGGGTGCCCG GCCTTCTGTG GAGCTCTCCT TCCAGCCCTC CCAGCCCCTC 
AGCAAGTCCA GCTCCTCCCC TGAGCTGCAG ACCCTGCAGG ACATACTCGG GGACCCCGGG 
GACAAGTCTG ATGTTGGCCG GCTCAGCCCT GAGGCCAAGG CCCGGTCACA GTCCGGGATT 
CTGGACGGGG TGGGTGCCTC CTGGTCAGCC CCAGGTGAAG AGAACCAGGG CCAGGGCCCC 
GCACAGCCCG AGGGTCCCTT GCCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCC 
AGAGGCTACA CCATCTCTGA TTCAGCCCCG TCACGCAGAG GCAAGAGGGT AGAGCGGGAC 
GCCTTCAGGA GCAGAGCTGG GGCTTCCAAC ACTGAGAAGG TGCCAGGCAT CAATCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCACCC TTCTTTGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTACCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTTC TCGACCAGAT TCCATCATAC 
GACACACACA AGATTGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAGCAG TGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCGTACAGG TACACGGAGT TCCTGACAGG CCTGGGCAAG 
CTCATTGAGC TCAAAGACTG CCAGCCGGAC AAGGTGTACC TGGGGGGCCT GGACGTGTGT 
GGCGAAGATG GCCAGTTCAC CTACTGCTGG CATGACGACA TCATGCAAGC CGTCTTCCAC 
ATTGCTACCC TGATGCCCAC CAAGGAAGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ACTTTGTGTC CATCGTCTAC AACGACTCTG GTGAGGACTT CAAGCTGGGC 
ACCATCCGAG GCCAGTTCAA CTTCGTCCAC GTGATCATCA CCCCCCTGGA CTATGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAAGGTC TTGTGGATAC TAGCGTGGCC 
AAGATCGTGT CTGACCGCAA CTTGCCTTTT GTGGCACGTC AGATGGCTTT GCATGCAAAT 
ATGGCCTCAC AAGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTATCC CTCCAAGTGG 
ATTGCCAGGC TTCGCCACAT CAAGCGACTC CGCCACCGGA TCCGCGAGGA AGCCCACTAC 
ACCAACCCCA GCCTGCCACT GATGCAGATG CACCCCCCGG GCCACCCCAA AGCCTCTGCT 
CAGGCGCCGG CAGAGCCTGT GCCCACGTAT GAGACAGGCC AGCGGAAGCG TCTCATCTCC 
TCTGTGGATG ACTTCACGGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621776.1
CDS	289..5598
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621719.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621719.3

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC 
GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA 
ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG 
TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC 
TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG 
GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT 
CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC 
AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA 
GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC 
AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT 
CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT 
CCGCCCCCGA GATCTAACAC GGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGTTGCCAA 
GGAAAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCGG TGGTTCTGGA GGAGGGAAAT 
TCAAGCGAGA CCAGTCTGCC AGTGGAGCAC ACGGAGCTGG AGGACTTCGA GGCGACACTA 
GGCACAGACC GGCGCTGTGG CCGCGCGGAA GCTTACAGCA GGTCGTCTTC AAGCTCCAGC 
CAGGAGGAGA AGTTCCCTGC AGAGGAGCTG GCTGCCGGAG GGATCCCCAT CGAACGAGCT 
GTCTCTTCCG AGGGTGCCCG GCCTTCTGTG GAGCTCTCCT TCCAGCCCTC CCAGCCCCTC 
AGCAAGTCCA GCTCCTCCCC TGAGCTGCAG ACCCTGCAGG ACATACTCGG GGACCCCGGG 
GACAAGTCTG ATGTTGGCCG GCTCAGCCCT GAGGCCAAGG CCCGGTCACA GTCCGGGATT 
CTGGACGGGG TGGGTGCCTC CTGGTCAGCC CCAGGTGAAG AGAACCAGGG CCAGGGCCCC 
GCACAGCCCG AGGGTCCCTT GCCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCC 
AGAGGCTACA CCATCTCTGA TTCAGCCCCG TCACGCAGAG GCAAGAGGGT AGAGCGGGAC 
GCCTTCAGGA GCAGAGCTGG GGCTTCCAAC ACTGAGAAGG TGCCAGGCAT CAATCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCACCC TTCTTTGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTACCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTTC TCGACCAGAT TCCATCATAC 
GACACACACA AGATTGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAGCAG TGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCGTACAGG TACACGGAGT TCCTGACAGG CCTGGGCAAG 
CTCATTGAGC TCAAAGACTG CCAGCCGGAC AAGGTGTACC TGGGGGGCCT GGACGTGTGT 
GGCGAAGATG GCCAGTTCAC CTACTGCTGG CATGACGACA TCATGCAAGC CGTCTTCCAC 
ATTGCTACCC TGATGCCCAC CAAGGAAGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ACTTTGTGTC CATCGTCTAC AACGACTCTG GTGAGGACTT CAAGCTGGGC 
ACCATCCGAG GCCAGTTCAA CTTCGTCCAC GTGATCATCA CCCCCCTGGA CTATGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAAGGTC TTGTGGATAC TAGCGTGGCC 
AAGATCGTGT CTGACCGCAA CTTGCCTTTT GTGGCACGTC AGATGGCTTT GCATGCAAAT 
ATGGCCTCAC AAGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTATCC CTCCAAGTGG 
ATTGCCAGGC TTCGCCACAT CAAGCGACTC CGCCACCGGA TCCGCGAGGA AGCCCACTAC 
ACCAACCCCA GCCTGCCACT GATGCAGATG CACCCCCCGG GCCACCCCAA AGCCTCTGCT 
CAGGCGCCGG CAGAGCCTGT GCCCACGTAT GAGACAGGCC AGCGGAAGCG TCTCATCTCC 
TCTGTGGATG ACTTCACGGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621719.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621776.1 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe08334
Clone ID Related Accession (Same CDS sequence)	XM_005621719.3 , XM_005621719.2
Accession Version	XM_005621719.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tuberin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621719.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC 
GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA 
ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG 
TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC 
TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG 
GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT 
CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC 
AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA 
GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC 
AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT 
CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT 
CCGCCCCCGA GATCTAACAC GGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGTTGCCAA 
GGAAAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCGG TGGTTCTGGA GGAGGGAAAT 
TCAAGCGAGA CCAGTCTGCC AGTGGAGCAC ACGGAGCTGG AGGACTTCGA GGCGACACTA 
GGCACAGACC GGCGCTGTGG CCGCGCGGAA GCTTACAGCA GGTCGTCTTC AAGCTCCAGC 
CAGGAGGAGA AGTTCCCTGC AGAGGAGCTG GCTGCCGGAG GGATCCCCAT CGAACGAGCT 
GTCTCTTCCG AGGGTGCCCG GCCTTCTGTG GAGCTCTCCT TCCAGCCCTC CCAGCCCCTC 
AGCAAGTCCA GCTCCTCCCC TGAGCTGCAG ACCCTGCAGG ACATACTCGG GGACCCCGGG 
GACAAGTCTG ATGTTGGCCG GCTCAGCCCT GAGGCCAAGG CCCGGTCACA GTCCGGGATT 
CTGGACGGGG TGGGTGCCTC CTGGTCAGCC CCAGGTGAAG AGAACCAGGG CCAGGGCCCC 
GCACAGCCCG AGGGTCCCTT GCCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCC 
AGAGGCTACA CCATCTCTGA TTCAGCCCCG TCACGCAGAG GCAAGAGGGT AGAGCGGGAC 
GCCTTCAGGA GCAGAGCTGG GGCTTCCAAC ACTGAGAAGG TGCCAGGCAT CAATCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCACCC TTCTTTGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTACCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTTC TCGACCAGAT TCCATCATAC 
GACACACACA AGATTGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAGCAG TGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCGTACAGG TACACGGAGT TCCTGACAGG CCTGGGCAAG 
CTCATTGAGC TCAAAGACTG CCAGCCGGAC AAGGTGTACC TGGGGGGCCT GGACGTGTGT 
GGCGAAGATG GCCAGTTCAC CTACTGCTGG CATGACGACA TCATGCAAGC CGTCTTCCAC 
ATTGCTACCC TGATGCCCAC CAAGGAAGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ACTTTGTGTC CATCGTCTAC AACGACTCTG GTGAGGACTT CAAGCTGGGC 
ACCATCCGAG GCCAGTTCAA CTTCGTCCAC GTGATCATCA CCCCCCTGGA CTATGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAAGGTC TTGTGGATAC TAGCGTGGCC 
AAGATCGTGT CTGACCGCAA CTTGCCTTTT GTGGCACGTC AGATGGCTTT GCATGCAAAT 
ATGGCCTCAC AAGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTATCC CTCCAAGTGG 
ATTGCCAGGC TTCGCCACAT CAAGCGACTC CGCCACCGGA TCCGCGAGGA AGCCCACTAC 
ACCAACCCCA GCCTGCCACT GATGCAGATG CACCCCCCGG GCCACCCCAA AGCCTCTGCT 
CAGGCGCCGG CAGAGCCTGT GCCCACGTAT GAGACAGGCC AGCGGAAGCG TCTCATCTCC 
TCTGTGGATG ACTTCACGGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621776.1
CDS	283..5592
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTVASFSSLYQSSCQGKLHRSISWADSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621719.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621719.2

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC 
GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA 
ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG 
TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC 
TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG 
GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT 
CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC 
AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA 
GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC 
AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT 
CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT 
CCGCCCCCGA GATCTAACAC GGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGTTGCCAA 
GGAAAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCGG TGGTTCTGGA GGAGGGAAAT 
TCAAGCGAGA CCAGTCTGCC AGTGGAGCAC ACGGAGCTGG AGGACTTCGA GGCGACACTA 
GGCACAGACC GGCGCTGTGG CCGCGCGGAA GCTTACAGCA GGTCGTCTTC AAGCTCCAGC 
CAGGAGGAGA AGTTCCCTGC AGAGGAGCTG GCTGCCGGAG GGATCCCCAT CGAACGAGCT 
GTCTCTTCCG AGGGTGCCCG GCCTTCTGTG GAGCTCTCCT TCCAGCCCTC CCAGCCCCTC 
AGCAAGTCCA GCTCCTCCCC TGAGCTGCAG ACCCTGCAGG ACATACTCGG GGACCCCGGG 
GACAAGTCTG ATGTTGGCCG GCTCAGCCCT GAGGCCAAGG CCCGGTCACA GTCCGGGATT 
CTGGACGGGG TGGGTGCCTC CTGGTCAGCC CCAGGTGAAG AGAACCAGGG CCAGGGCCCC 
GCACAGCCCG AGGGTCCCTT GCCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCC 
AGAGGCTACA CCATCTCTGA TTCAGCCCCG TCACGCAGAG GCAAGAGGGT AGAGCGGGAC 
GCCTTCAGGA GCAGAGCTGG GGCTTCCAAC ACTGAGAAGG TGCCAGGCAT CAATCCCAGC 
TTTGTGTTCC TGCAGCTCTA CCACTCACCC TTCTTTGGTG ACGAGTCCAA CAAGCCCATC 
CTTCTACCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTTC TCGACCAGAT TCCATCATAC 
GACACACACA AGATTGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAGCAG TGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCGTACAGG TACACGGAGT TCCTGACAGG CCTGGGCAAG 
CTCATTGAGC TCAAAGACTG CCAGCCGGAC AAGGTGTACC TGGGGGGCCT GGACGTGTGT 
GGCGAAGATG GCCAGTTCAC CTACTGCTGG CATGACGACA TCATGCAAGC CGTCTTCCAC 
ATTGCTACCC TGATGCCCAC CAAGGAAGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC 
CTGGGCAATG ACTTTGTGTC CATCGTCTAC AACGACTCTG GTGAGGACTT CAAGCTGGGC 
ACCATCCGAG GCCAGTTCAA CTTCGTCCAC GTGATCATCA CCCCCCTGGA CTATGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAAGGTC TTGTGGATAC TAGCGTGGCC 
AAGATCGTGT CTGACCGCAA CTTGCCTTTT GTGGCACGTC AGATGGCTTT GCATGCAAAT 
ATGGCCTCAC AAGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTATCC CTCCAAGTGG 
ATTGCCAGGC TTCGCCACAT CAAGCGACTC CGCCACCGGA TCCGCGAGGA AGCCCACTAC 
ACCAACCCCA GCCTGCCACT GATGCAGATG CACCCCCCGG GCCACCCCAA AGCCTCTGCT 
CAGGCGCCGG CAGAGCCTGT GCCCACGTAT GAGACAGGCC AGCGGAAGCG TCTCATCTCC 
TCTGTGGATG ACTTCACGGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621720.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621777.1 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe08335
Clone ID Related Accession (Same CDS sequence)	XM_005621720.2 , XM_005621720.3
Accession Version	XM_005621720.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tuberin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT 
GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG 
GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG 
AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG 
GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA 
CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC 
ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC 
AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG 
GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC 
AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG 
CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC 
CCTCCGCCCC CGAGATCTAA CACGGACTCT GCGGTGGTTC TGGAGGAGGG AAATTCAAGC 
GAGACCAGTC TGCCAGTGGA GCACACGGAG CTGGAGGACT TCGAGGCGAC ACTAGGCACA 
GACCGGCGCT GTGGCCGCGC GGAAGCTTAC AGCAGGTCGT CTTCAAGCTC CAGCCAGGAG 
GAGAAGTTCC CTGCAGAGGA GCTGGCTGCC GGAGGGATCC CCATCGAACG AGCTGTCTCT 
TCCGAGGGTG CCCGGCCTTC TGTGGAGCTC TCCTTCCAGC CCTCCCAGCC CCTCAGCAAG 
TCCAGCTCCT CCCCTGAGCT GCAGACCCTG CAGGACATAC TCGGGGACCC CGGGGACAAG 
TCTGATGTTG GCCGGCTCAG CCCTGAGGCC AAGGCCCGGT CACAGTCCGG GATTCTGGAC 
GGGGTGGGTG CCTCCTGGTC AGCCCCAGGT GAAGAGAACC AGGGCCAGGG CCCCGCACAG 
CCCGAGGGTC CCTTGCCTTC CAGCTGCCCC CGCTCCCCCA GTGGCCTGCG GCCCAGAGGC 
TACACCATCT CTGATTCAGC CCCGTCACGC AGAGGCAAGA GGGTAGAGCG GGACGCCTTC 
AGGAGCAGAG CTGGGGCTTC CAACACTGAG AAGGTGCCAG GCATCAATCC CAGCTTTGTG 
TTCCTGCAGC TCTACCACTC ACCCTTCTTT GGTGACGAGT CCAACAAGCC CATCCTTCTA 
CCCAATGAGT CCTTCGAGCG GTCAGTGCAG CTTCTCGACC AGATTCCATC ATACGACACA 
CACAAGATTG CCGTCCTGTA CGTGGGAGAA GGCCAGAGCA GCAGTGAGCT CGCCATCCTG 
TCCAATGAGC ATGGCTCGTA CAGGTACACG GAGTTCCTGA CAGGCCTGGG CAAGCTCATT 
GAGCTCAAAG ACTGCCAGCC GGACAAGGTG TACCTGGGGG GCCTGGACGT GTGTGGCGAA 
GATGGCCAGT TCACCTACTG CTGGCATGAC GACATCATGC AAGCCGTCTT CCACATTGCT 
ACCCTGATGC CCACCAAGGA AGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AATGACTTTG TGTCCATCGT CTACAACGAC TCTGGTGAGG ACTTCAAGCT GGGCACCATC 
CGAGGCCAGT TCAACTTCGT CCACGTGATC ATCACCCCCC TGGACTATGA GTGCAACCTG 
GTGTCCCTGC AGTGCAGGAA AGACATGGAA GGTCTTGTGG ATACTAGCGT GGCCAAGATC 
GTGTCTGACC GCAACTTGCC TTTTGTGGCA CGTCAGATGG CTTTGCATGC AAATATGGCC 
TCACAAGTGC ATCATAGCCG CTCCAACCCC ACCGACATCT ATCCCTCCAA GTGGATTGCC 
AGGCTTCGCC ACATCAAGCG ACTCCGCCAC CGGATCCGCG AGGAAGCCCA CTACACCAAC 
CCCAGCCTGC CACTGATGCA GATGCACCCC CCGGGCCACC CCAAAGCCTC TGCTCAGGCG 
CCGGCAGAGC CTGTGCCCAC GTATGAGACA GGCCAGCGGA AGCGTCTCAT CTCCTCTGTG 
GATGACTTCA CGGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621777.1
CDS	282..5525
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621720.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621720.2

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT 
GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG 
GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG 
AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG 
GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA 
CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC 
ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC 
AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG 
GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC 
AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG 
CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC 
CCTCCGCCCC CGAGATCTAA CACGGACTCT GCGGTGGTTC TGGAGGAGGG AAATTCAAGC 
GAGACCAGTC TGCCAGTGGA GCACACGGAG CTGGAGGACT TCGAGGCGAC ACTAGGCACA 
GACCGGCGCT GTGGCCGCGC GGAAGCTTAC AGCAGGTCGT CTTCAAGCTC CAGCCAGGAG 
GAGAAGTTCC CTGCAGAGGA GCTGGCTGCC GGAGGGATCC CCATCGAACG AGCTGTCTCT 
TCCGAGGGTG CCCGGCCTTC TGTGGAGCTC TCCTTCCAGC CCTCCCAGCC CCTCAGCAAG 
TCCAGCTCCT CCCCTGAGCT GCAGACCCTG CAGGACATAC TCGGGGACCC CGGGGACAAG 
TCTGATGTTG GCCGGCTCAG CCCTGAGGCC AAGGCCCGGT CACAGTCCGG GATTCTGGAC 
GGGGTGGGTG CCTCCTGGTC AGCCCCAGGT GAAGAGAACC AGGGCCAGGG CCCCGCACAG 
CCCGAGGGTC CCTTGCCTTC CAGCTGCCCC CGCTCCCCCA GTGGCCTGCG GCCCAGAGGC 
TACACCATCT CTGATTCAGC CCCGTCACGC AGAGGCAAGA GGGTAGAGCG GGACGCCTTC 
AGGAGCAGAG CTGGGGCTTC CAACACTGAG AAGGTGCCAG GCATCAATCC CAGCTTTGTG 
TTCCTGCAGC TCTACCACTC ACCCTTCTTT GGTGACGAGT CCAACAAGCC CATCCTTCTA 
CCCAATGAGT CCTTCGAGCG GTCAGTGCAG CTTCTCGACC AGATTCCATC ATACGACACA 
CACAAGATTG CCGTCCTGTA CGTGGGAGAA GGCCAGAGCA GCAGTGAGCT CGCCATCCTG 
TCCAATGAGC ATGGCTCGTA CAGGTACACG GAGTTCCTGA CAGGCCTGGG CAAGCTCATT 
GAGCTCAAAG ACTGCCAGCC GGACAAGGTG TACCTGGGGG GCCTGGACGT GTGTGGCGAA 
GATGGCCAGT TCACCTACTG CTGGCATGAC GACATCATGC AAGCCGTCTT CCACATTGCT 
ACCCTGATGC CCACCAAGGA AGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AATGACTTTG TGTCCATCGT CTACAACGAC TCTGGTGAGG ACTTCAAGCT GGGCACCATC 
CGAGGCCAGT TCAACTTCGT CCACGTGATC ATCACCCCCC TGGACTATGA GTGCAACCTG 
GTGTCCCTGC AGTGCAGGAA AGACATGGAA GGTCTTGTGG ATACTAGCGT GGCCAAGATC 
GTGTCTGACC GCAACTTGCC TTTTGTGGCA CGTCAGATGG CTTTGCATGC AAATATGGCC 
TCACAAGTGC ATCATAGCCG CTCCAACCCC ACCGACATCT ATCCCTCCAA GTGGATTGCC 
AGGCTTCGCC ACATCAAGCG ACTCCGCCAC CGGATCCGCG AGGAAGCCCA CTACACCAAC 
CCCAGCCTGC CACTGATGCA GATGCACCCC CCGGGCCACC CCAAAGCCTC TGCTCAGGCG 
CCGGCAGAGC CTGTGCCCAC GTATGAGACA GGCCAGCGGA AGCGTCTCAT CTCCTCTGTG 
GATGACTTCA CGGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005621720.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621777.1 Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe08335
Clone ID Related Accession (Same CDS sequence)	XM_005621720.2 , XM_005621720.3
Accession Version	XM_005621720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT 
GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG 
GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG 
AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG 
GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA 
CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC 
ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC 
AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG 
GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC 
AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG 
CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC 
CCTCCGCCCC CGAGATCTAA CACGGACTCT GCGGTGGTTC TGGAGGAGGG AAATTCAAGC 
GAGACCAGTC TGCCAGTGGA GCACACGGAG CTGGAGGACT TCGAGGCGAC ACTAGGCACA 
GACCGGCGCT GTGGCCGCGC GGAAGCTTAC AGCAGGTCGT CTTCAAGCTC CAGCCAGGAG 
GAGAAGTTCC CTGCAGAGGA GCTGGCTGCC GGAGGGATCC CCATCGAACG AGCTGTCTCT 
TCCGAGGGTG CCCGGCCTTC TGTGGAGCTC TCCTTCCAGC CCTCCCAGCC CCTCAGCAAG 
TCCAGCTCCT CCCCTGAGCT GCAGACCCTG CAGGACATAC TCGGGGACCC CGGGGACAAG 
TCTGATGTTG GCCGGCTCAG CCCTGAGGCC AAGGCCCGGT CACAGTCCGG GATTCTGGAC 
GGGGTGGGTG CCTCCTGGTC AGCCCCAGGT GAAGAGAACC AGGGCCAGGG CCCCGCACAG 
CCCGAGGGTC CCTTGCCTTC CAGCTGCCCC CGCTCCCCCA GTGGCCTGCG GCCCAGAGGC 
TACACCATCT CTGATTCAGC CCCGTCACGC AGAGGCAAGA GGGTAGAGCG GGACGCCTTC 
AGGAGCAGAG CTGGGGCTTC CAACACTGAG AAGGTGCCAG GCATCAATCC CAGCTTTGTG 
TTCCTGCAGC TCTACCACTC ACCCTTCTTT GGTGACGAGT CCAACAAGCC CATCCTTCTA 
CCCAATGAGT CCTTCGAGCG GTCAGTGCAG CTTCTCGACC AGATTCCATC ATACGACACA 
CACAAGATTG CCGTCCTGTA CGTGGGAGAA GGCCAGAGCA GCAGTGAGCT CGCCATCCTG 
TCCAATGAGC ATGGCTCGTA CAGGTACACG GAGTTCCTGA CAGGCCTGGG CAAGCTCATT 
GAGCTCAAAG ACTGCCAGCC GGACAAGGTG TACCTGGGGG GCCTGGACGT GTGTGGCGAA 
GATGGCCAGT TCACCTACTG CTGGCATGAC GACATCATGC AAGCCGTCTT CCACATTGCT 
ACCCTGATGC CCACCAAGGA AGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AATGACTTTG TGTCCATCGT CTACAACGAC TCTGGTGAGG ACTTCAAGCT GGGCACCATC 
CGAGGCCAGT TCAACTTCGT CCACGTGATC ATCACCCCCC TGGACTATGA GTGCAACCTG 
GTGTCCCTGC AGTGCAGGAA AGACATGGAA GGTCTTGTGG ATACTAGCGT GGCCAAGATC 
GTGTCTGACC GCAACTTGCC TTTTGTGGCA CGTCAGATGG CTTTGCATGC AAATATGGCC 
TCACAAGTGC ATCATAGCCG CTCCAACCCC ACCGACATCT ATCCCTCCAA GTGGATTGCC 
AGGCTTCGCC ACATCAAGCG ACTCCGCCAC CGGATCCGCG AGGAAGCCCA CTACACCAAC 
CCCAGCCTGC CACTGATGCA GATGCACCCC CCGGGCCACC CCAAAGCCTC TGCTCAGGCG 
CCGGCAGAGC CTGTGCCCAC GTATGAGACA GGCCAGCGGA AGCGTCTCAT CTCCTCTGTG 
GATGACTTCA CGGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621777.1
CDS	289..5532
Translation	MAKPTSKDSGLKEKFRILLGLGTPRPNPRSAEGKQTEFIITADILRELSVECGINNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANEDLHERLEVFKALTDNGRHITYLEEELAGFVLQWMDIGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSPELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAVYSASLEDVKTAVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSEKKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDQQGGPELSSDPGMHVRQAKEAPAKLESQAGQQVCRGARDRVRSMSGGHGLRVGALDTPASHLPGGPTSPGTQAALASKSDRASAGTRFPAQEKTSLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPPPPPRSNTDSAVVLEEGNSSETSLPVEHTELEDFEATLGTDRRCGRAEAYSRSSSSSSQEEKFPAEELAAGGIPIERAVSSEGARPSVELSFQPSQPLSKSSSSPELQTLQDILGDPGDKSDVGRLSPEAKARSQSGILDGVGASWSAPGEENQGQGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYTNPSLPLMQMHPPGHPKASAQAPAEPVPTYETGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005621720.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621720.3

ORF Insert Sequence:

ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC 
ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG 
ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG 
CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA 
GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG 
GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG 
GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG 
GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT 
GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC 
ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC 
GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG 
CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG 
GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG 
ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG 
CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC 
TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC 
CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC 
AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC 
CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG 
TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC 
TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA 
GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT 
CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG 
AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC 
AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT 
AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG 
CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC 
AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT 
GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG 
CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG 
GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG 
GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG 
CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC 
GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG 
GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC 
GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC 
ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC 
GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT 
GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG 
GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG 
AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG 
GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA 
CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC 
ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC 
AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG 
GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC 
AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG 
CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC 
CCTCCGCCCC CGAGATCTAA CACGGACTCT GCGGTGGTTC TGGAGGAGGG AAATTCAAGC 
GAGACCAGTC TGCCAGTGGA GCACACGGAG CTGGAGGACT TCGAGGCGAC ACTAGGCACA 
GACCGGCGCT GTGGCCGCGC GGAAGCTTAC AGCAGGTCGT CTTCAAGCTC CAGCCAGGAG 
GAGAAGTTCC CTGCAGAGGA GCTGGCTGCC GGAGGGATCC CCATCGAACG AGCTGTCTCT 
TCCGAGGGTG CCCGGCCTTC TGTGGAGCTC TCCTTCCAGC CCTCCCAGCC CCTCAGCAAG 
TCCAGCTCCT CCCCTGAGCT GCAGACCCTG CAGGACATAC TCGGGGACCC CGGGGACAAG 
TCTGATGTTG GCCGGCTCAG CCCTGAGGCC AAGGCCCGGT CACAGTCCGG GATTCTGGAC 
GGGGTGGGTG CCTCCTGGTC AGCCCCAGGT GAAGAGAACC AGGGCCAGGG CCCCGCACAG 
CCCGAGGGTC CCTTGCCTTC CAGCTGCCCC CGCTCCCCCA GTGGCCTGCG GCCCAGAGGC 
TACACCATCT CTGATTCAGC CCCGTCACGC AGAGGCAAGA GGGTAGAGCG GGACGCCTTC 
AGGAGCAGAG CTGGGGCTTC CAACACTGAG AAGGTGCCAG GCATCAATCC CAGCTTTGTG 
TTCCTGCAGC TCTACCACTC ACCCTTCTTT GGTGACGAGT CCAACAAGCC CATCCTTCTA 
CCCAATGAGT CCTTCGAGCG GTCAGTGCAG CTTCTCGACC AGATTCCATC ATACGACACA 
CACAAGATTG CCGTCCTGTA CGTGGGAGAA GGCCAGAGCA GCAGTGAGCT CGCCATCCTG 
TCCAATGAGC ATGGCTCGTA CAGGTACACG GAGTTCCTGA CAGGCCTGGG CAAGCTCATT 
GAGCTCAAAG ACTGCCAGCC GGACAAGGTG TACCTGGGGG GCCTGGACGT GTGTGGCGAA 
GATGGCCAGT TCACCTACTG CTGGCATGAC GACATCATGC AAGCCGTCTT CCACATTGCT 
ACCCTGATGC CCACCAAGGA AGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AATGACTTTG TGTCCATCGT CTACAACGAC TCTGGTGAGG ACTTCAAGCT GGGCACCATC 
CGAGGCCAGT TCAACTTCGT CCACGTGATC ATCACCCCCC TGGACTATGA GTGCAACCTG 
GTGTCCCTGC AGTGCAGGAA AGACATGGAA GGTCTTGTGG ATACTAGCGT GGCCAAGATC 
GTGTCTGACC GCAACTTGCC TTTTGTGGCA CGTCAGATGG CTTTGCATGC AAATATGGCC 
TCACAAGTGC ATCATAGCCG CTCCAACCCC ACCGACATCT ATCCCTCCAA GTGGATTGCC 
AGGCTTCGCC ACATCAAGCG ACTCCGCCAC CGGATCCGCG AGGAAGCCCA CTACACCAAC 
CCCAGCCTGC CACTGATGCA GATGCACCCC CCGGGCCACC CCAAAGCCTC TGCTCAGGCG 
CCGGCAGAGC CTGTGCCCAC GTATGAGACA GGCCAGCGGA AGCGTCTCAT CTCCTCTGTG 
GATGACTTCA CGGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC2
Entrez Gene ID	479883
Full Name	TSC complex subunit 2
General protein information	Preferred Names TSC complex subunit 2 Names tuberin tuberous sclerosis 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Pan troglodytes (chimpanzee)	TSC2	XP_523477.3
Mus musculus (house mouse)	Tsc2	NP_001034452.1
Rattus norvegicus (Norway rat)	Tsc2	NP_036812.2
Gallus gallus (chicken)	TSC2	XP_003642168.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011123	XP_309523.4
Canis lupus familiaris (dog)	TSC2	XP_852047.2
Bos taurus (cattle)	TSC2	XP_003587856.2
Drosophila melanogaster (fruit fly)	gig	NP_524177.1
Homo sapiens (human)	TSC2	NP_000539.2
	TSC2	XP_001083256.1
Danio rerio (zebrafish)	tsc2	XP_695912.6
Xenopus tropicalis (tropical clawed frog)	tsc2	NP_001123795.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム