TSC2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015469256.1	XP_015324742.1	tuberin isoform X4
XM_010825645.2	XP_010823947.2	tuberin isoform X1
XM_003584005.4	XP_003584053.2	tuberin isoform X7
XM_015469258.1	XP_015324744.1	tuberin isoform X1
XM_010819198.2	XP_010817500.2	tuberin isoform X1
XM_024985434.1	XP_024841202.1	tuberin isoform X3
XM_024985435.1	XP_024841203.1	tuberin isoform X4
XM_015460251.2	XP_015315737.2	tuberin isoform X7
XM_003584006.4	XP_003584054.2	tuberin isoform X3
XM_015460254.1	XP_015315740.1	tuberin isoform X1
XM_015460253.1	XP_015315739.1	tuberin isoform X5
XM_015460256.1	XP_015315742.1	tuberin isoform X7
XM_015460257.1	XP_015315743.1	tuberin isoform X1
XM_024985438.1	XP_024841206.1	tuberin isoform X8
XM_015460252.2	XP_015315738.2	tuberin isoform X11
XM_015460255.2	XP_015315741.2	tuberin isoform X12
XM_010819198.3	XP_010817500.3	tuberin isoform X4
XM_005196829.3	XP_005196886.2	tuberin isoform X6
XM_015460252.1	XP_015315738.1	tuberin isoform X4
XM_015460250.1	XP_015315736.1	tuberin isoform X2
XM_024985433.1	XP_024841201.1	tuberin isoform X2
XM_024985436.1	XP_024841204.1	tuberin isoform X5
XM_015460251.1	XP_015315737.1	tuberin isoform X3
XM_015469255.1	XP_015324741.1	tuberin isoform X2
XM_015460254.2	XP_015315740.1	tuberin isoform X1
XM_024985437.1	XP_024841205.1	tuberin isoform X6
XM_024985440.1	XP_024841208.1	tuberin isoform X10
XM_024985441.1	XP_024841209.1	tuberin isoform X9
XM_015460257.2	XP_015315743.1	tuberin isoform X1
XM_015460255.1	XP_015315741.1	tuberin isoform X6
XM_003584007.4	XP_003584055.2	tuberin isoform X1
XM_015469257.1	XP_015324743.1	tuberin isoform X5
XM_024985439.1	XP_024841207.1	tuberin isoform X9
XM_015460256.2	XP_015315742.2	tuberin isoform X13

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230687	XM_015460254.2 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230687	XM_015460257.2 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230688	XM_024985433.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230689	XM_024985434.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230690	XM_010819198.3 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230690	XM_024985435.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230691	XM_024985436.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230692	XM_024985437.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230693	XM_015460251.2 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230694	XM_024985438.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230695	XM_024985439.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230695	XM_024985441.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230696	XM_024985440.1 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230697	XM_015460252.2 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230698	XM_015460255.2 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230699	XM_015460256.2 Latest version!	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa88433	XM_015460257.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88433	XM_003584007.4 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88433	XM_015460254.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88433	XM_010819198.2 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88433	XM_010825645.2 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88433	XM_015469258.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88434	XM_015469255.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88434	XM_015460250.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88435	XM_003584006.4 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88435	XM_015460251.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88436	XM_015460252.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88436	XM_015469256.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88437	XM_015460253.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88437	XM_015469257.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88438	XM_015460255.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88438	XM_005196829.3 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88439	XM_015460256.1 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa88439	XM_003584005.4 Latest version!	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

TSC2 ( XM_015460254.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315740.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230687
Clone ID Related Accession (Same CDS sequence)	XM_015460254.2 , XM_015460257.2
Accession Version	XM_015460254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315740.1
CDS	461..5404
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460254.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460254.2

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460257.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315743.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X16, mRNA.

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CloneID	OBa230687
Clone ID Related Accession (Same CDS sequence)	XM_015460254.2 , XM_015460257.2
Accession Version	XM_015460257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315743.1
CDS	315..5258
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460257.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460257.2

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985433.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841201.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa230688
Clone ID Related Accession (Same CDS sequence)	XM_024985433.1
Accession Version	XM_024985433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGGATCC AGACCTCTCT CACGAGCGCC AGCTTGGGGT CTGCAGACGA GAACTCGATG 
GCCCAGGCCG ACGACAACTT GAAAAACCTC CACCTGGAGC TCACAGAGAC GTGTCTGGAC 
ATGATGGCCA GATATGTGTT CTCCAACTTC ACGGCGGTCC CCAAGAGGTC CCCCGTGGGA 
GAGTTCCTCC TGGCTGGTGG CAGGACCAAA ACCTGGCTGG TTGGGAACAA GCTTGTCACC 
GTGACGACAA GCGTGGGGAC CGGGACCCGG TCACTGCTAG GCCTGGACTC TGGAGAGCTG 
CAGGGTGGCC CGGAGTTGAG CTCTGACCCC AGCATGCATG TGAGACAGAC AAAGGAGGCA 
CCCGCCAAGC TGGAGTCCCA AGCTGGGCAG CAGGTGTACC ACGGAGCCCG GGACCGGGTC 
CGCTCCATGT CCGGGGGCCA TGGCCTTCGC GTGGGTGCCT TGGACGCTCC AGCCTGCTAC 
TTCCCCAGCA GCCCCACGTC CCCGGGCTCC CAGACCGCTC CAGCCAGCCA GCCCGAGAAG 
GCCTCAGCTG GCAGCCCGCT CCCAGCACAG AAGGAGAAGA CAAACCTGGC CGCTTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCAGAGATC CTGGTCCGCA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCCT CGGACATCAA CAGCATGCCG 
CTGCAGGAGC TGTCCAATGC CCTCATGGCC GCCGAGCGCT TCAAGGAGCG CCGCGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCGGCG GCCGGCTCAG CCAAGCCATC CCCGCCCCCA 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTGCCAGT CCAGGTGCCA AGGAAAGCTG 
CACAGGAGCA TTTCCTGGGC AGATTCTGCG GTGGTCCTGG AGGAGGGAGG TCCAGGTGAG 
GCTGGTTTGT CTGCAGAGCC TCCCGAGTTG GAAGATTTTG AGGCGACACT GGGCTCCGAC 
GGGCGCTGTG GGCGCAGCGA CGCTTTCAGC AGGTCATCTT CCACCTCCAG CCAGGAGGAG 
AAGTCATTCC ATGCCGAGGA GTTGCCTCCT GGGGGCATCC CCATCGAGCG GGCGGTCTCG 
GAGGGCTCCC GGGCCTCCGT GGACCTCGCC TTCCAGCCCT CTCAGCCCCT GAGCAAGTCC 
AGCTCGTCGC CTGAACTGCA GACCCTGCAG GACATCCTGG GGGACCCCGG GGACAAGGCT 
GAGGTTGGCC GGCTGAGCCC CGAGGCCAAG GCCCGGTCGC AGTCAGGGAT CCTGGACGGG 
GAAGGTGCCG CCTGGTCAGC CCCGGGTGAG GAACGCCGGG GTCGGGGCCC TGCCCAGCCA 
GAGGGCCCCC TGCCTTCCAG CTGTCCTCGC TCCCCCAGCG GGCTGCGGCC CCGAGGCTAC 
ACCATCTCGG ATTCGGCCCC GTCACGCAGG GGCAAGAGGG TGGAGAGGGA TGCCTTCAAG 
AACCGAGCAG GGACCTCCAA CACCGAGAAG GTGCCAGGCA TCAACCCCAG CTTCGTGTTC 
CTGCAGCTCT ACCACTCACC GTTCTTTGGT GACGAGTCCA ACAAGCCAAT CCTTTTGCCT 
AACGAGTCTT TCGAACGATC AGTGCAGCTC CTTGATCAGA TCCCGTCGTA CGACACGCAC 
AAGATCGCGG TCCTGTACGT GGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACAGAG TTCCTCACGG GGCTGGGCAA GCTCATCGAG 
CTCAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG CGGCGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CTGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAC 
GACTTCGTGT CCATCGTCTA CAACGACTCT GGCGAGGACT TCAAGTTGGG CACCATCAAG 
GGCCAATTCA ACTTCGTCCA CGTGATTATC ACGCCCCTGG ACTACGAGTG CAACCTGGTG 
TCTCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGATA CCAGCATGGC CAAGATCGTG 
TCTGACCGAA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCCAA TATGGCCTCG 
CAGGTGCACC ATAGCCGCTC CAATCCCACC GACATTTATC CCTCCAAGTG GATTGCCAGG 
CTCCGCCACA TCAAGCGGCT GCGCCAGCGG ATCCGTGAGG AAGCCCACTA CTCAAGCGCC 
AGCCTGCCCC TGATGCAGAC ACACCCTCCT GGTCATGCCA AGGCTCCGGC ACAGGCCCCG 
GCCGAGTCCA CGCCCACCTA TGAGACGGGC CAGCGGAAAC GCCTGGTCTC CTCTGTGGAC 
GACTTCACTG AGTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841201.1
CDS	461..5401
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985433.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985433.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGGATCC AGACCTCTCT CACGAGCGCC AGCTTGGGGT CTGCAGACGA GAACTCGATG 
GCCCAGGCCG ACGACAACTT GAAAAACCTC CACCTGGAGC TCACAGAGAC GTGTCTGGAC 
ATGATGGCCA GATATGTGTT CTCCAACTTC ACGGCGGTCC CCAAGAGGTC CCCCGTGGGA 
GAGTTCCTCC TGGCTGGTGG CAGGACCAAA ACCTGGCTGG TTGGGAACAA GCTTGTCACC 
GTGACGACAA GCGTGGGGAC CGGGACCCGG TCACTGCTAG GCCTGGACTC TGGAGAGCTG 
CAGGGTGGCC CGGAGTTGAG CTCTGACCCC AGCATGCATG TGAGACAGAC AAAGGAGGCA 
CCCGCCAAGC TGGAGTCCCA AGCTGGGCAG CAGGTGTACC ACGGAGCCCG GGACCGGGTC 
CGCTCCATGT CCGGGGGCCA TGGCCTTCGC GTGGGTGCCT TGGACGCTCC AGCCTGCTAC 
TTCCCCAGCA GCCCCACGTC CCCGGGCTCC CAGACCGCTC CAGCCAGCCA GCCCGAGAAG 
GCCTCAGCTG GCAGCCCGCT CCCAGCACAG AAGGAGAAGA CAAACCTGGC CGCTTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCAGAGATC CTGGTCCGCA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCCT CGGACATCAA CAGCATGCCG 
CTGCAGGAGC TGTCCAATGC CCTCATGGCC GCCGAGCGCT TCAAGGAGCG CCGCGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCGGCG GCCGGCTCAG CCAAGCCATC CCCGCCCCCA 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTGCCAGT CCAGGTGCCA AGGAAAGCTG 
CACAGGAGCA TTTCCTGGGC AGATTCTGCG GTGGTCCTGG AGGAGGGAGG TCCAGGTGAG 
GCTGGTTTGT CTGCAGAGCC TCCCGAGTTG GAAGATTTTG AGGCGACACT GGGCTCCGAC 
GGGCGCTGTG GGCGCAGCGA CGCTTTCAGC AGGTCATCTT CCACCTCCAG CCAGGAGGAG 
AAGTCATTCC ATGCCGAGGA GTTGCCTCCT GGGGGCATCC CCATCGAGCG GGCGGTCTCG 
GAGGGCTCCC GGGCCTCCGT GGACCTCGCC TTCCAGCCCT CTCAGCCCCT GAGCAAGTCC 
AGCTCGTCGC CTGAACTGCA GACCCTGCAG GACATCCTGG GGGACCCCGG GGACAAGGCT 
GAGGTTGGCC GGCTGAGCCC CGAGGCCAAG GCCCGGTCGC AGTCAGGGAT CCTGGACGGG 
GAAGGTGCCG CCTGGTCAGC CCCGGGTGAG GAACGCCGGG GTCGGGGCCC TGCCCAGCCA 
GAGGGCCCCC TGCCTTCCAG CTGTCCTCGC TCCCCCAGCG GGCTGCGGCC CCGAGGCTAC 
ACCATCTCGG ATTCGGCCCC GTCACGCAGG GGCAAGAGGG TGGAGAGGGA TGCCTTCAAG 
AACCGAGCAG GGACCTCCAA CACCGAGAAG GTGCCAGGCA TCAACCCCAG CTTCGTGTTC 
CTGCAGCTCT ACCACTCACC GTTCTTTGGT GACGAGTCCA ACAAGCCAAT CCTTTTGCCT 
AACGAGTCTT TCGAACGATC AGTGCAGCTC CTTGATCAGA TCCCGTCGTA CGACACGCAC 
AAGATCGCGG TCCTGTACGT GGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACAGAG TTCCTCACGG GGCTGGGCAA GCTCATCGAG 
CTCAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG CGGCGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CTGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAC 
GACTTCGTGT CCATCGTCTA CAACGACTCT GGCGAGGACT TCAAGTTGGG CACCATCAAG 
GGCCAATTCA ACTTCGTCCA CGTGATTATC ACGCCCCTGG ACTACGAGTG CAACCTGGTG 
TCTCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGATA CCAGCATGGC CAAGATCGTG 
TCTGACCGAA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCCAA TATGGCCTCG 
CAGGTGCACC ATAGCCGCTC CAATCCCACC GACATTTATC CCTCCAAGTG GATTGCCAGG 
CTCCGCCACA TCAAGCGGCT GCGCCAGCGG ATCCGTGAGG AAGCCCACTA CTCAAGCGCC 
AGCCTGCCCC TGATGCAGAC ACACCCTCCT GGTCATGCCA AGGCTCCGGC ACAGGCCCCG 
GCCGAGTCCA CGCCCACCTA TGAGACGGGC CAGCGGAAAC GCCTGGTCTC CTCTGTGGAC 
GACTTCACTG AGTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985434.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841202.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa230689
Clone ID Related Accession (Same CDS sequence)	XM_024985434.1
Accession Version	XM_024985434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841202.1
CDS	461..5335
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985434.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985434.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985435.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841203.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OBa230690
Clone ID Related Accession (Same CDS sequence)	XM_024985435.1 , XM_010819198.3
Accession Version	XM_024985435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAACTCAA GGAGAGCTCT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACCT CTCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC GATGGCCCAG GCCGACGACA ACTTGAAAAA CCTCCACCTG 
GAGCTCACAG AGACGTGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACGGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACCGTGACG ACAAGCGTGG GGACCGGGAC CCGGTCACTG 
CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT GGCCCGGAGT TGAGCTCTGA CCCCAGCATG 
CATGTGAGAC AGACAAAGGA GGCACCCGCC AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG 
TACCACGGAG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCATGGCCT TCGCGTGGGT 
GCCTTGGACG CTCCAGCCTG CTACTTCCCC AGCAGCCCCA CGTCCCCGGG CTCCCAGACC 
GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG 
AAGACAAACC TGGCCGCTTA CGTGCCCCTG CTGACCCAGG GCTGGGCAGA GATCCTGGTC 
CGCAGGCCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAGCAT GCCGCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGCGA CACAGCCCTG TACAAGTCGC TGTCGGTGCC GGCGGCCGGC 
TCAGCCAAGC CATCCCCGCC CCCACGCTCC AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCAGGT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGATTC TGCGGTGGTC 
CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT 
TTTGAGGCGA CACTGGGCTC CGACGGGCGC TGTGGGCGCA GCGACGCTTT CAGCAGGTCA 
TCTTCCACCT CCAGCCAGGA GGAGAAGTCA TTCCATGCCG AGGAGTTGCC TCCTGGGGGC 
ATCCCCATCG AGCGGGCGGT CTCGGAGGGC TCCCGGGCCT CCGTGGACCT CGCCTTCCAG 
CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG TCGCCTGAAC TGCAGACCCT GCAGGACATC 
CTGGGGGACC CCGGGGACAA GGCTGAGGTT GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG 
TCGCAGTCAG GGATCCTGGA CGGGGAAGGT GCCGCCTGGT CAGCCCCGGG TGAGGAACGC 
CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC 
AGCGGGCTGC GGCCCCGAGG CTACACCATC TCGGATTCGG CCCCGTCACG CAGGGGCAAG 
AGGGTGGAGA GGGATGCCTT CAAGAACCGA GCAGGGACCT CCAACACCGA GAAGGTGCCA 
GGCATCAACC CCAGCTTCGT GTTCCTGCAG CTCTACCACT CACCGTTCTT TGGTGACGAG 
TCCAACAAGC CAATCCTTTT GCCTAACGAG TCTTTCGAAC GATCAGTGCA GCTCCTTGAT 
CAGATCCCGT CGTACGACAC GCACAAGATC GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AACAGCGAGC TCGCCATCCT GTCCAACGAG CATGGCTCCT ACAGGTACAC AGAGTTCCTC 
ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG GACTGCCAGC CGGACAAGGT GTACCTGGGC 
GGCCTGGACG TGTGCGGCGA GGATGGCCAG TTCACCTACT GCTGGCACGA CGACATCATG 
CAAGCTGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAACGACTTC GTGTCCATCG TCTACAACGA CTCTGGCGAG 
GACTTCAAGT TGGGCACCAT CAAGGGCCAA TTCAACTTCG TCCACGTGAT TATCACGCCC 
CTGGACTACG AGTGCAACCT GGTGTCTCTG CAGTGCAGGA AAGACATGGA GGGCCTTGTG 
GATACCAGCA TGGCCAAGAT CGTGTCTGAC CGAAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCACG CCAATATGGC CTCGCAGGTG CACCATAGCC GCTCCAATCC CACCGACATT 
TATCCCTCCA AGTGGATTGC CAGGCTCCGC CACATCAAGC GGCTGCGCCA GCGGATCCGT 
GAGGAAGCCC ACTACTCAAG CGCCAGCCTG CCCCTGATGC AGACACACCC TCCTGGTCAT 
GCCAAGGCTC CGGCACAGGC CCCGGCCGAG TCCACGCCCA CCTATGAGAC GGGCCAGCGG 
AAACGCCTGG TCTCCTCTGT GGACGACTTC ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841203.1
CDS	497..5941
Translation	MAKPASKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985435.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985435.1

ORF Insert Sequence:

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAACTCAA GGAGAGCTCT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACCT CTCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC GATGGCCCAG GCCGACGACA ACTTGAAAAA CCTCCACCTG 
GAGCTCACAG AGACGTGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACGGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACCGTGACG ACAAGCGTGG GGACCGGGAC CCGGTCACTG 
CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT GGCCCGGAGT TGAGCTCTGA CCCCAGCATG 
CATGTGAGAC AGACAAAGGA GGCACCCGCC AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG 
TACCACGGAG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCATGGCCT TCGCGTGGGT 
GCCTTGGACG CTCCAGCCTG CTACTTCCCC AGCAGCCCCA CGTCCCCGGG CTCCCAGACC 
GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG 
AAGACAAACC TGGCCGCTTA CGTGCCCCTG CTGACCCAGG GCTGGGCAGA GATCCTGGTC 
CGCAGGCCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAGCAT GCCGCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGCGA CACAGCCCTG TACAAGTCGC TGTCGGTGCC GGCGGCCGGC 
TCAGCCAAGC CATCCCCGCC CCCACGCTCC AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCAGGT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGATTC TGCGGTGGTC 
CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT 
TTTGAGGCGA CACTGGGCTC CGACGGGCGC TGTGGGCGCA GCGACGCTTT CAGCAGGTCA 
TCTTCCACCT CCAGCCAGGA GGAGAAGTCA TTCCATGCCG AGGAGTTGCC TCCTGGGGGC 
ATCCCCATCG AGCGGGCGGT CTCGGAGGGC TCCCGGGCCT CCGTGGACCT CGCCTTCCAG 
CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG TCGCCTGAAC TGCAGACCCT GCAGGACATC 
CTGGGGGACC CCGGGGACAA GGCTGAGGTT GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG 
TCGCAGTCAG GGATCCTGGA CGGGGAAGGT GCCGCCTGGT CAGCCCCGGG TGAGGAACGC 
CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC 
AGCGGGCTGC GGCCCCGAGG CTACACCATC TCGGATTCGG CCCCGTCACG CAGGGGCAAG 
AGGGTGGAGA GGGATGCCTT CAAGAACCGA GCAGGGACCT CCAACACCGA GAAGGTGCCA 
GGCATCAACC CCAGCTTCGT GTTCCTGCAG CTCTACCACT CACCGTTCTT TGGTGACGAG 
TCCAACAAGC CAATCCTTTT GCCTAACGAG TCTTTCGAAC GATCAGTGCA GCTCCTTGAT 
CAGATCCCGT CGTACGACAC GCACAAGATC GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AACAGCGAGC TCGCCATCCT GTCCAACGAG CATGGCTCCT ACAGGTACAC AGAGTTCCTC 
ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG GACTGCCAGC CGGACAAGGT GTACCTGGGC 
GGCCTGGACG TGTGCGGCGA GGATGGCCAG TTCACCTACT GCTGGCACGA CGACATCATG 
CAAGCTGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAACGACTTC GTGTCCATCG TCTACAACGA CTCTGGCGAG 
GACTTCAAGT TGGGCACCAT CAAGGGCCAA TTCAACTTCG TCCACGTGAT TATCACGCCC 
CTGGACTACG AGTGCAACCT GGTGTCTCTG CAGTGCAGGA AAGACATGGA GGGCCTTGTG 
GATACCAGCA TGGCCAAGAT CGTGTCTGAC CGAAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCACG CCAATATGGC CTCGCAGGTG CACCATAGCC GCTCCAATCC CACCGACATT 
TATCCCTCCA AGTGGATTGC CAGGCTCCGC CACATCAAGC GGCTGCGCCA GCGGATCCGT 
GAGGAAGCCC ACTACTCAAG CGCCAGCCTG CCCCTGATGC AGACACACCC TCCTGGTCAT 
GCCAAGGCTC CGGCACAGGC CCCGGCCGAG TCCACGCCCA CCTATGAGAC GGGCCAGCGG 
AAACGCCTGG TCTCCTCTGT GGACGACTTC ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_010819198.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817500.3 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X13, mRNA.

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CloneID	OBa230690
Clone ID Related Accession (Same CDS sequence)	XM_024985435.1 , XM_010819198.3
Accession Version	XM_010819198.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819198.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAACTCAA GGAGAGCTCT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACCT CTCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC GATGGCCCAG GCCGACGACA ACTTGAAAAA CCTCCACCTG 
GAGCTCACAG AGACGTGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACGGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACCGTGACG ACAAGCGTGG GGACCGGGAC CCGGTCACTG 
CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT GGCCCGGAGT TGAGCTCTGA CCCCAGCATG 
CATGTGAGAC AGACAAAGGA GGCACCCGCC AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG 
TACCACGGAG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCATGGCCT TCGCGTGGGT 
GCCTTGGACG CTCCAGCCTG CTACTTCCCC AGCAGCCCCA CGTCCCCGGG CTCCCAGACC 
GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG 
AAGACAAACC TGGCCGCTTA CGTGCCCCTG CTGACCCAGG GCTGGGCAGA GATCCTGGTC 
CGCAGGCCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAGCAT GCCGCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGCGA CACAGCCCTG TACAAGTCGC TGTCGGTGCC GGCGGCCGGC 
TCAGCCAAGC CATCCCCGCC CCCACGCTCC AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCAGGT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGATTC TGCGGTGGTC 
CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT 
TTTGAGGCGA CACTGGGCTC CGACGGGCGC TGTGGGCGCA GCGACGCTTT CAGCAGGTCA 
TCTTCCACCT CCAGCCAGGA GGAGAAGTCA TTCCATGCCG AGGAGTTGCC TCCTGGGGGC 
ATCCCCATCG AGCGGGCGGT CTCGGAGGGC TCCCGGGCCT CCGTGGACCT CGCCTTCCAG 
CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG TCGCCTGAAC TGCAGACCCT GCAGGACATC 
CTGGGGGACC CCGGGGACAA GGCTGAGGTT GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG 
TCGCAGTCAG GGATCCTGGA CGGGGAAGGT GCCGCCTGGT CAGCCCCGGG TGAGGAACGC 
CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC 
AGCGGGCTGC GGCCCCGAGG CTACACCATC TCGGATTCGG CCCCGTCACG CAGGGGCAAG 
AGGGTGGAGA GGGATGCCTT CAAGAACCGA GCAGGGACCT CCAACACCGA GAAGGTGCCA 
GGCATCAACC CCAGCTTCGT GTTCCTGCAG CTCTACCACT CACCGTTCTT TGGTGACGAG 
TCCAACAAGC CAATCCTTTT GCCTAACGAG TCTTTCGAAC GATCAGTGCA GCTCCTTGAT 
CAGATCCCGT CGTACGACAC GCACAAGATC GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AACAGCGAGC TCGCCATCCT GTCCAACGAG CATGGCTCCT ACAGGTACAC AGAGTTCCTC 
ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG GACTGCCAGC CGGACAAGGT GTACCTGGGC 
GGCCTGGACG TGTGCGGCGA GGATGGCCAG TTCACCTACT GCTGGCACGA CGACATCATG 
CAAGCTGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAACGACTTC GTGTCCATCG TCTACAACGA CTCTGGCGAG 
GACTTCAAGT TGGGCACCAT CAAGGGCCAA TTCAACTTCG TCCACGTGAT TATCACGCCC 
CTGGACTACG AGTGCAACCT GGTGTCTCTG CAGTGCAGGA AAGACATGGA GGGCCTTGTG 
GATACCAGCA TGGCCAAGAT CGTGTCTGAC CGAAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCACG CCAATATGGC CTCGCAGGTG CACCATAGCC GCTCCAATCC CACCGACATT 
TATCCCTCCA AGTGGATTGC CAGGCTCCGC CACATCAAGC GGCTGCGCCA GCGGATCCGT 
GAGGAAGCCC ACTACTCAAG CGCCAGCCTG CCCCTGATGC AGACACACCC TCCTGGTCAT 
GCCAAGGCTC CGGCACAGGC CCCGGCCGAG TCCACGCCCA CCTATGAGAC GGGCCAGCGG 
AAACGCCTGG TCTCCTCTGT GGACGACTTC ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817500.3
CDS	163..5607
Translation	MAKPASKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_010819198.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819198.3

ORF Insert Sequence:

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAACTCAA GGAGAGCTCT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACCT CTCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC GATGGCCCAG GCCGACGACA ACTTGAAAAA CCTCCACCTG 
GAGCTCACAG AGACGTGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACGGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACCGTGACG ACAAGCGTGG GGACCGGGAC CCGGTCACTG 
CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT GGCCCGGAGT TGAGCTCTGA CCCCAGCATG 
CATGTGAGAC AGACAAAGGA GGCACCCGCC AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG 
TACCACGGAG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCATGGCCT TCGCGTGGGT 
GCCTTGGACG CTCCAGCCTG CTACTTCCCC AGCAGCCCCA CGTCCCCGGG CTCCCAGACC 
GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG 
AAGACAAACC TGGCCGCTTA CGTGCCCCTG CTGACCCAGG GCTGGGCAGA GATCCTGGTC 
CGCAGGCCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAGCAT GCCGCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGCGA CACAGCCCTG TACAAGTCGC TGTCGGTGCC GGCGGCCGGC 
TCAGCCAAGC CATCCCCGCC CCCACGCTCC AACACAGTGG CCTCTTTCTC CTCCCTGTGC 
CAGTCCAGGT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGATTC TGCGGTGGTC 
CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT 
TTTGAGGCGA CACTGGGCTC CGACGGGCGC TGTGGGCGCA GCGACGCTTT CAGCAGGTCA 
TCTTCCACCT CCAGCCAGGA GGAGAAGTCA TTCCATGCCG AGGAGTTGCC TCCTGGGGGC 
ATCCCCATCG AGCGGGCGGT CTCGGAGGGC TCCCGGGCCT CCGTGGACCT CGCCTTCCAG 
CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG TCGCCTGAAC TGCAGACCCT GCAGGACATC 
CTGGGGGACC CCGGGGACAA GGCTGAGGTT GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG 
TCGCAGTCAG GGATCCTGGA CGGGGAAGGT GCCGCCTGGT CAGCCCCGGG TGAGGAACGC 
CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC 
AGCGGGCTGC GGCCCCGAGG CTACACCATC TCGGATTCGG CCCCGTCACG CAGGGGCAAG 
AGGGTGGAGA GGGATGCCTT CAAGAACCGA GCAGGGACCT CCAACACCGA GAAGGTGCCA 
GGCATCAACC CCAGCTTCGT GTTCCTGCAG CTCTACCACT CACCGTTCTT TGGTGACGAG 
TCCAACAAGC CAATCCTTTT GCCTAACGAG TCTTTCGAAC GATCAGTGCA GCTCCTTGAT 
CAGATCCCGT CGTACGACAC GCACAAGATC GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC 
AACAGCGAGC TCGCCATCCT GTCCAACGAG CATGGCTCCT ACAGGTACAC AGAGTTCCTC 
ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG GACTGCCAGC CGGACAAGGT GTACCTGGGC 
GGCCTGGACG TGTGCGGCGA GGATGGCCAG TTCACCTACT GCTGGCACGA CGACATCATG 
CAAGCTGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGT 
GACAAGAAGC GCCACCTGGG CAACGACTTC GTGTCCATCG TCTACAACGA CTCTGGCGAG 
GACTTCAAGT TGGGCACCAT CAAGGGCCAA TTCAACTTCG TCCACGTGAT TATCACGCCC 
CTGGACTACG AGTGCAACCT GGTGTCTCTG CAGTGCAGGA AAGACATGGA GGGCCTTGTG 
GATACCAGCA TGGCCAAGAT CGTGTCTGAC CGAAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCACG CCAATATGGC CTCGCAGGTG CACCATAGCC GCTCCAATCC CACCGACATT 
TATCCCTCCA AGTGGATTGC CAGGCTCCGC CACATCAAGC GGCTGCGCCA GCGGATCCGT 
GAGGAAGCCC ACTACTCAAG CGCCAGCCTG CCCCTGATGC AGACACACCC TCCTGGTCAT 
GCCAAGGCTC CGGCACAGGC CCCGGCCGAG TCCACGCCCA CCTATGAGAC GGGCCAGCGG 
AAACGCCTGG TCTCCTCTGT GGACGACTTC ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985436.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841204.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OBa230691
Clone ID Related Accession (Same CDS sequence)	XM_024985436.1
Accession Version	XM_024985436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGTGG CCTCTTTCTC CTCCCTGTGC CAGTCCAGGT GCCAAGGAAA GCTGCACAGG 
AGCATTTCCT GGGCAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841204.1
CDS	461..5275
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985436.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985436.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGTGG CCTCTTTCTC CTCCCTGTGC CAGTCCAGGT GCCAAGGAAA GCTGCACAGG 
AGCATTTCCT GGGCAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985437.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841205.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OBa230692
Clone ID Related Accession (Same CDS sequence)	XM_024985437.1
Accession Version	XM_024985437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG TCCAGGTGCC AAGGAAAGCT GCACAGGAGC 
ATTTCCTGGG CAGATTCTGC GGTGGTCCTG GAGGAGGGAG GTCCAGGTGA GGCTGGTTTG 
TCTGCAGAGC CTCCCGAGTT GGAAGATTTT GAGGCGACAC TGGGCTCCGA CGGGCGCTGT 
GGGCGCAGCG ACGCTTTCAG CAGGTCATCT TCCACCTCCA GCCAGGAGGA GAAGTCATTC 
CATGCCGAGG AGTTGCCTCC TGGGGGCATC CCCATCGAGC GGGCGGTCTC GGAGGGCTCC 
CGGGCCTCCG TGGACCTCGC CTTCCAGCCC TCTCAGCCCC TGAGCAAGTC CAGCTCGTCG 
CCTGAACTGC AGACCCTGCA GGACATCCTG GGGGACCCCG GGGACAAGGC TGAGGTTGGC 
CGGCTGAGCC CCGAGGCCAA GGCCCGGTCG CAGTCAGGGA TCCTGGACGG GGAAGGTGCC 
GCCTGGTCAG CCCCGGGTGA GGAACGCCGG GGTCGGGGCC CTGCCCAGCC AGAGGGCCCC 
CTGCCTTCCA GCTGTCCTCG CTCCCCCAGC GGGCTGCGGC CCCGAGGCTA CACCATCTCG 
GATTCGGCCC CGTCACGCAG GGGCAAGAGG GTGGAGAGGG ATGCCTTCAA GAACCGAGCA 
GGGACCTCCA ACACCGAGAA GGTGCCAGGC ATCAACCCCA GCTTCGTGTT CCTGCAGCTC 
TACCACTCAC CGTTCTTTGG TGACGAGTCC AACAAGCCAA TCCTTTTGCC TAACGAGTCT 
TTCGAACGAT CAGTGCAGCT CCTTGATCAG ATCCCGTCGT ACGACACGCA CAAGATCGCG 
GTCCTGTACG TGGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT 
GGCTCCTACA GGTACACAGA GTTCCTCACG GGGCTGGGCA AGCTCATCGA GCTCAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GCGGCGAGGA TGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCTGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGTGAC AAGAAGCGCC ACCTGGGCAA CGACTTCGTG 
TCCATCGTCT ACAACGACTC TGGCGAGGAC TTCAAGTTGG GCACCATCAA GGGCCAATTC 
AACTTCGTCC ACGTGATTAT CACGCCCCTG GACTACGAGT GCAACCTGGT GTCTCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAT ACCAGCATGG CCAAGATCGT GTCTGACCGA 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCCA ATATGGCCTC GCAGGTGCAC 
CATAGCCGCT CCAATCCCAC CGACATTTAT CCCTCCAAGT GGATTGCCAG GCTCCGCCAC 
ATCAAGCGGC TGCGCCAGCG GATCCGTGAG GAAGCCCACT ACTCAAGCGC CAGCCTGCCC 
CTGATGCAGA CACACCCTCC TGGTCATGCC AAGGCTCCGG CACAGGCCCC GGCCGAGTCC 
ACGCCCACCT ATGAGACGGG CCAGCGGAAA CGCCTGGTCT CCTCTGTGGA CGACTTCACT 
GAGTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841205.1
CDS	461..5272
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985437.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985437.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG TCCAGGTGCC AAGGAAAGCT GCACAGGAGC 
ATTTCCTGGG CAGATTCTGC GGTGGTCCTG GAGGAGGGAG GTCCAGGTGA GGCTGGTTTG 
TCTGCAGAGC CTCCCGAGTT GGAAGATTTT GAGGCGACAC TGGGCTCCGA CGGGCGCTGT 
GGGCGCAGCG ACGCTTTCAG CAGGTCATCT TCCACCTCCA GCCAGGAGGA GAAGTCATTC 
CATGCCGAGG AGTTGCCTCC TGGGGGCATC CCCATCGAGC GGGCGGTCTC GGAGGGCTCC 
CGGGCCTCCG TGGACCTCGC CTTCCAGCCC TCTCAGCCCC TGAGCAAGTC CAGCTCGTCG 
CCTGAACTGC AGACCCTGCA GGACATCCTG GGGGACCCCG GGGACAAGGC TGAGGTTGGC 
CGGCTGAGCC CCGAGGCCAA GGCCCGGTCG CAGTCAGGGA TCCTGGACGG GGAAGGTGCC 
GCCTGGTCAG CCCCGGGTGA GGAACGCCGG GGTCGGGGCC CTGCCCAGCC AGAGGGCCCC 
CTGCCTTCCA GCTGTCCTCG CTCCCCCAGC GGGCTGCGGC CCCGAGGCTA CACCATCTCG 
GATTCGGCCC CGTCACGCAG GGGCAAGAGG GTGGAGAGGG ATGCCTTCAA GAACCGAGCA 
GGGACCTCCA ACACCGAGAA GGTGCCAGGC ATCAACCCCA GCTTCGTGTT CCTGCAGCTC 
TACCACTCAC CGTTCTTTGG TGACGAGTCC AACAAGCCAA TCCTTTTGCC TAACGAGTCT 
TTCGAACGAT CAGTGCAGCT CCTTGATCAG ATCCCGTCGT ACGACACGCA CAAGATCGCG 
GTCCTGTACG TGGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT 
GGCTCCTACA GGTACACAGA GTTCCTCACG GGGCTGGGCA AGCTCATCGA GCTCAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GCGGCGAGGA TGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCTGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGTGAC AAGAAGCGCC ACCTGGGCAA CGACTTCGTG 
TCCATCGTCT ACAACGACTC TGGCGAGGAC TTCAAGTTGG GCACCATCAA GGGCCAATTC 
AACTTCGTCC ACGTGATTAT CACGCCCCTG GACTACGAGT GCAACCTGGT GTCTCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAT ACCAGCATGG CCAAGATCGT GTCTGACCGA 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCCA ATATGGCCTC GCAGGTGCAC 
CATAGCCGCT CCAATCCCAC CGACATTTAT CCCTCCAAGT GGATTGCCAG GCTCCGCCAC 
ATCAAGCGGC TGCGCCAGCG GATCCGTGAG GAAGCCCACT ACTCAAGCGC CAGCCTGCCC 
CTGATGCAGA CACACCCTCC TGGTCATGCC AAGGCTCCGG CACAGGCCCC GGCCGAGTCC 
ACGCCCACCT ATGAGACGGG CCAGCGGAAA CGCCTGGTCT CCTCTGTGGA CGACTTCACT 
GAGTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460251.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315737.2 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.

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CloneID	OBa230693
Clone ID Related Accession (Same CDS sequence)	XM_015460251.2
Accession Version	XM_015460251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAACTCAA GGAGAGCTCT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACCT CTCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC GATGGCCCAG GCCGACGACA ACTTGAAAAA CCTCCACCTG 
GAGCTCACAG AGACGTGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACGGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACCGTGACG ACAAGCGTGG GGACCGGGAC CCGGTCACTG 
CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT GGCCCGGAGT TGAGCTCTGA CCCCAGCATG 
CATGTGAGAC AGACAAAGGA GGCACCCGCC AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG 
TACCACGGAG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCATGGCCT TCGCGTGGGT 
GCCTTGGACG CTCCAGCCTG CTACTTCCCC AGCAGCCCCA CGTCCCCGGG CTCCCAGACC 
GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG 
AAGACAAACC TGGCCGCTTA CGTGCCCCTG CTGACCCAGG GCTGGGCAGA GATCCTGGTC 
CGCAGGCCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAGCAT GCCGCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGCGA CACAGCCCTG TACAAGTCGC TGTCGGTGCC GGCGGCCGGC 
TCAGCCAAGC CATCCCCGCC CCCACGCTCC AACACAGATT CTGCGGTGGT CCTGGAGGAG 
GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG 
ACACTGGGCT CCGACGGGCG CTGTGGGCGC AGCGACGCTT TCAGCAGGTC ATCTTCCACC 
TCCAGCCAGG AGGAGAAGTC ATTCCATGCC GAGGAGTTGC CTCCTGGGGG CATCCCCATC 
GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC TCCGTGGACC TCGCCTTCCA GCCCTCTCAG 
CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA CTGCAGACCC TGCAGGACAT CCTGGGGGAC 
CCCGGGGACA AGGCTGAGGT TGGCCGGCTG AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ACGGGGAAGG TGCCGCCTGG TCAGCCCCGG GTGAGGAACG CCGGGGTCGG 
GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG 
CGGCCCCGAG GCTACACCAT CTCGGATTCG GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG 
AGGGATGCCT TCAAGAACCG AGCAGGGACC TCCAACACCG AGAAGGTGCC AGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCACCGTTCT TTGGTGACGA GTCCAACAAG 
CCAATCCTTT TGCCTAACGA GTCTTTCGAA CGATCAGTGC AGCTCCTTGA TCAGATCCCG 
TCGTACGACA CGCACAAGAT CGCGGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGCGAG 
CTCGCCATCC TGTCCAACGA GCATGGCTCC TACAGGTACA CAGAGTTCCT CACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG CGGCCTGGAC 
GTGTGCGGCG AGGATGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCTGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAACGACTT CGTGTCCATC GTCTACAACG ACTCTGGCGA GGACTTCAAG 
TTGGGCACCA TCAAGGGCCA ATTCAACTTC GTCCACGTGA TTATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCTCT GCAGTGCAGG AAAGACATGG AGGGCCTTGT GGATACCAGC 
ATGGCCAAGA TCGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC 
GCCAATATGG CCTCGCAGGT GCACCATAGC CGCTCCAATC CCACCGACAT TTATCCCTCC 
AAGTGGATTG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AGCGGATCCG TGAGGAAGCC 
CACTACTCAA GCGCCAGCCT GCCCCTGATG CAGACACACC CTCCTGGTCA TGCCAAGGCT 
CCGGCACAGG CCCCGGCCGA GTCCACGCCC ACCTATGAGA CGGGCCAGCG GAAACGCCTG 
GTCTCCTCTG TGGACGACTT CACTGAGTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315737.2
CDS	139..5514
Translation	MAKPASKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460251.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460251.2

ORF Insert Sequence:

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAACTCAA GGAGAGCTCT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACCT CTCTCACGAG CGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC GATGGCCCAG GCCGACGACA ACTTGAAAAA CCTCCACCTG 
GAGCTCACAG AGACGTGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACGGCG 
GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACCGTGACG ACAAGCGTGG GGACCGGGAC CCGGTCACTG 
CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT GGCCCGGAGT TGAGCTCTGA CCCCAGCATG 
CATGTGAGAC AGACAAAGGA GGCACCCGCC AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG 
TACCACGGAG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCATGGCCT TCGCGTGGGT 
GCCTTGGACG CTCCAGCCTG CTACTTCCCC AGCAGCCCCA CGTCCCCGGG CTCCCAGACC 
GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG 
AAGACAAACC TGGCCGCTTA CGTGCCCCTG CTGACCCAGG GCTGGGCAGA GATCCTGGTC 
CGCAGGCCCA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC 
TCCTCGGACA TCAACAGCAT GCCGCTGCAG GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG 
CGCTTCAAGG AGCGCCGCGA CACAGCCCTG TACAAGTCGC TGTCGGTGCC GGCGGCCGGC 
TCAGCCAAGC CATCCCCGCC CCCACGCTCC AACACAGATT CTGCGGTGGT CCTGGAGGAG 
GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG 
ACACTGGGCT CCGACGGGCG CTGTGGGCGC AGCGACGCTT TCAGCAGGTC ATCTTCCACC 
TCCAGCCAGG AGGAGAAGTC ATTCCATGCC GAGGAGTTGC CTCCTGGGGG CATCCCCATC 
GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC TCCGTGGACC TCGCCTTCCA GCCCTCTCAG 
CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA CTGCAGACCC TGCAGGACAT CCTGGGGGAC 
CCCGGGGACA AGGCTGAGGT TGGCCGGCTG AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ACGGGGAAGG TGCCGCCTGG TCAGCCCCGG GTGAGGAACG CCGGGGTCGG 
GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG 
CGGCCCCGAG GCTACACCAT CTCGGATTCG GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG 
AGGGATGCCT TCAAGAACCG AGCAGGGACC TCCAACACCG AGAAGGTGCC AGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCACCGTTCT TTGGTGACGA GTCCAACAAG 
CCAATCCTTT TGCCTAACGA GTCTTTCGAA CGATCAGTGC AGCTCCTTGA TCAGATCCCG 
TCGTACGACA CGCACAAGAT CGCGGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGCGAG 
CTCGCCATCC TGTCCAACGA GCATGGCTCC TACAGGTACA CAGAGTTCCT CACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG CGGCCTGGAC 
GTGTGCGGCG AGGATGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCTGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAACGACTT CGTGTCCATC GTCTACAACG ACTCTGGCGA GGACTTCAAG 
TTGGGCACCA TCAAGGGCCA ATTCAACTTC GTCCACGTGA TTATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCTCT GCAGTGCAGG AAAGACATGG AGGGCCTTGT GGATACCAGC 
ATGGCCAAGA TCGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC 
GCCAATATGG CCTCGCAGGT GCACCATAGC CGCTCCAATC CCACCGACAT TTATCCCTCC 
AAGTGGATTG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AGCGGATCCG TGAGGAAGCC 
CACTACTCAA GCGCCAGCCT GCCCCTGATG CAGACACACC CTCCTGGTCA TGCCAAGGCT 
CCGGCACAGG CCCCGGCCGA GTCCACGCCC ACCTATGAGA CGGGCCAGCG GAAACGCCTG 
GTCTCCTCTG TGGACGACTT CACTGAGTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985438.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841206.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X8, mRNA.

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CloneID	OBa230694
Clone ID Related Accession (Same CDS sequence)	XM_024985438.1
Accession Version	XM_024985438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAACTCAA GGAGAGCTCT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACCTCTC TCACGAGCGC CAGCTTGGGG 
TCTGCAGACG AGAACTCGAT GGCCCAGGCC GACGACAACT TGAAAAACCT CCACCTGGAG 
CTCACAGAGA CGTGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACGGCGGTC 
CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC CGTGACGACA AGCGTGGGGA CCGGGACCCG GTCACTGCTA 
GGCCTGGACT CTGGAGAGCT GCAGGGTGGC CCGGAGTTGA GCTCTGACCC CAGCATGCAT 
GTGAGACAGA CAAAGGAGGC ACCCGCCAAG CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC 
CACGGAGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC 
TTGGACGCTC CAGCCTGCTA CTTCCCCAGC AGCCCCACGT CCCCGGGCTC CCAGACCGCT 
CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT GGCAGCCCGC TCCCAGCACA GAAGGAGAAG 
ACAAACCTGG CCGCTTACGT GCCCCTGCTG ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCGGACATCA ACAGCATGCC GCTGCAGGAG CTGTCCAATG CCCTCATGGC CGCCGAGCGC 
TTCAAGGAGC GCCGCGACAC AGCCCTGTAC AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA 
GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC ACAGATTCTG CGGTGGTCCT GGAGGAGGGA 
GGTCCAGGTG AGGCTGGTTT GTCTGCAGAG CCTCCCGAGT TGGAAGATTT TGAGGCGACA 
CTGGGCTCCG ACGGGCGCTG TGGGCGCAGC GACGCTTTCA GCAGGTCATC TTCCACCTCC 
AGCCAGGAGG AGAAGTCATT CCATGCCGAG GAGTTGCCTC CTGGGGGCAT CCCCATCGAG 
CGGGCGGTCT CGGAGGGCTC CCGGGCCTCC GTGGACCTCG CCTTCCAGCC CTCTCAGCCC 
CTGAGCAAGT CCAGCTCGTC GCCTGAACTG CAGACCCTGC AGGACATCCT GGGGGACCCC 
GGGGACAAGG CTGAGGTTGG CCGGCTGAGC CCCGAGGCCA AGGCCCGGTC GCAGTCAGGG 
ATCCTGGACG GGGAAGGTGC CGCCTGGTCA GCCCCGGGTG AGGAACGCCG GGGTCGGGGC 
CCTGCCCAGC CAGAGGGCCC CCTGCCTTCC AGCTGTCCTC GCTCCCCCAG CGGGCTGCGG 
CCCCGAGGCT ACACCATCTC GGATTCGGCC CCGTCACGCA GGGGCAAGAG GGTGGAGAGG 
GATGCCTTCA AGAACCGAGC AGGGACCTCC AACACCGAGA AGGTGCCAGG CATCAACCCC 
AGCTTCGTGT TCCTGCAGCT CTACCACTCA CCGTTCTTTG GTGACGAGTC CAACAAGCCA 
ATCCTTTTGC CTAACGAGTC TTTCGAACGA TCAGTGCAGC TCCTTGATCA GATCCCGTCG 
TACGACACGC ACAAGATCGC GGTCCTGTAC GTGGGAGAAG GCCAGAGCAA CAGCGAGCTC 
GCCATCCTGT CCAACGAGCA TGGCTCCTAC AGGTACACAG AGTTCCTCAC GGGGCTGGGC 
AAGCTCATCG AGCTCAAGGA CTGCCAGCCG GACAAGGTGT ACCTGGGCGG CCTGGACGTG 
TGCGGCGAGG ATGGCCAGTT CACCTACTGC TGGCACGACG ACATCATGCA AGCTGTCTTC 
CACATCGCCA CCCTGATGCC CACCAAGGAC GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ACGACTTCGT GTCCATCGTC TACAACGACT CTGGCGAGGA CTTCAAGTTG 
GGCACCATCA AGGGCCAATT CAACTTCGTC CACGTGATTA TCACGCCCCT GGACTACGAG 
TGCAACCTGG TGTCTCTGCA GTGCAGGAAA GACATGGAGG GCCTTGTGGA TACCAGCATG 
GCCAAGATCG TGTCTGACCG AAACCTGCCC TTCGTGGCCC GCCAGATGGC CCTGCACGCC 
AATATGGCCT CGCAGGTGCA CCATAGCCGC TCCAATCCCA CCGACATTTA TCCCTCCAAG 
TGGATTGCCA GGCTCCGCCA CATCAAGCGG CTGCGCCAGC GGATCCGTGA GGAAGCCCAC 
TACTCAAGCG CCAGCCTGCC CCTGATGCAG ACACACCCTC CTGGTCATGC CAAGGCTCCG 
GCACAGGCCC CGGCCGAGTC CACGCCCACC TATGAGACGG GCCAGCGGAA ACGCCTGGTC 
TCCTCTGTGG ACGACTTCAC TGAGTTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841206.1
CDS	139..5511
Translation	MAKPASKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985438.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985438.1

ORF Insert Sequence:

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAACTCAA GGAGAGCTCT GCAGCCGATG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACCTCTC TCACGAGCGC CAGCTTGGGG 
TCTGCAGACG AGAACTCGAT GGCCCAGGCC GACGACAACT TGAAAAACCT CCACCTGGAG 
CTCACAGAGA CGTGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACGGCGGTC 
CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC CGTGACGACA AGCGTGGGGA CCGGGACCCG GTCACTGCTA 
GGCCTGGACT CTGGAGAGCT GCAGGGTGGC CCGGAGTTGA GCTCTGACCC CAGCATGCAT 
GTGAGACAGA CAAAGGAGGC ACCCGCCAAG CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC 
CACGGAGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC 
TTGGACGCTC CAGCCTGCTA CTTCCCCAGC AGCCCCACGT CCCCGGGCTC CCAGACCGCT 
CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT GGCAGCCCGC TCCCAGCACA GAAGGAGAAG 
ACAAACCTGG CCGCTTACGT GCCCCTGCTG ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC 
TCGGACATCA ACAGCATGCC GCTGCAGGAG CTGTCCAATG CCCTCATGGC CGCCGAGCGC 
TTCAAGGAGC GCCGCGACAC AGCCCTGTAC AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA 
GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC ACAGATTCTG CGGTGGTCCT GGAGGAGGGA 
GGTCCAGGTG AGGCTGGTTT GTCTGCAGAG CCTCCCGAGT TGGAAGATTT TGAGGCGACA 
CTGGGCTCCG ACGGGCGCTG TGGGCGCAGC GACGCTTTCA GCAGGTCATC TTCCACCTCC 
AGCCAGGAGG AGAAGTCATT CCATGCCGAG GAGTTGCCTC CTGGGGGCAT CCCCATCGAG 
CGGGCGGTCT CGGAGGGCTC CCGGGCCTCC GTGGACCTCG CCTTCCAGCC CTCTCAGCCC 
CTGAGCAAGT CCAGCTCGTC GCCTGAACTG CAGACCCTGC AGGACATCCT GGGGGACCCC 
GGGGACAAGG CTGAGGTTGG CCGGCTGAGC CCCGAGGCCA AGGCCCGGTC GCAGTCAGGG 
ATCCTGGACG GGGAAGGTGC CGCCTGGTCA GCCCCGGGTG AGGAACGCCG GGGTCGGGGC 
CCTGCCCAGC CAGAGGGCCC CCTGCCTTCC AGCTGTCCTC GCTCCCCCAG CGGGCTGCGG 
CCCCGAGGCT ACACCATCTC GGATTCGGCC CCGTCACGCA GGGGCAAGAG GGTGGAGAGG 
GATGCCTTCA AGAACCGAGC AGGGACCTCC AACACCGAGA AGGTGCCAGG CATCAACCCC 
AGCTTCGTGT TCCTGCAGCT CTACCACTCA CCGTTCTTTG GTGACGAGTC CAACAAGCCA 
ATCCTTTTGC CTAACGAGTC TTTCGAACGA TCAGTGCAGC TCCTTGATCA GATCCCGTCG 
TACGACACGC ACAAGATCGC GGTCCTGTAC GTGGGAGAAG GCCAGAGCAA CAGCGAGCTC 
GCCATCCTGT CCAACGAGCA TGGCTCCTAC AGGTACACAG AGTTCCTCAC GGGGCTGGGC 
AAGCTCATCG AGCTCAAGGA CTGCCAGCCG GACAAGGTGT ACCTGGGCGG CCTGGACGTG 
TGCGGCGAGG ATGGCCAGTT CACCTACTGC TGGCACGACG ACATCATGCA AGCTGTCTTC 
CACATCGCCA CCCTGATGCC CACCAAGGAC GTGGACAAGC ACCGCTGTGA CAAGAAGCGC 
CACCTGGGCA ACGACTTCGT GTCCATCGTC TACAACGACT CTGGCGAGGA CTTCAAGTTG 
GGCACCATCA AGGGCCAATT CAACTTCGTC CACGTGATTA TCACGCCCCT GGACTACGAG 
TGCAACCTGG TGTCTCTGCA GTGCAGGAAA GACATGGAGG GCCTTGTGGA TACCAGCATG 
GCCAAGATCG TGTCTGACCG AAACCTGCCC TTCGTGGCCC GCCAGATGGC CCTGCACGCC 
AATATGGCCT CGCAGGTGCA CCATAGCCGC TCCAATCCCA CCGACATTTA TCCCTCCAAG 
TGGATTGCCA GGCTCCGCCA CATCAAGCGG CTGCGCCAGC GGATCCGTGA GGAAGCCCAC 
TACTCAAGCG CCAGCCTGCC CCTGATGCAG ACACACCCTC CTGGTCATGC CAAGGCTCCG 
GCACAGGCCC CGGCCGAGTC CACGCCCACC TATGAGACGG GCCAGCGGAA ACGCCTGGTC 
TCCTCTGTGG ACGACTTCAC TGAGTTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985441.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841209.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X15, mRNA.

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CloneID	OBa230695
Clone ID Related Accession (Same CDS sequence)	XM_024985441.1 , XM_024985439.1
Accession Version	XM_024985441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGATT CTGCGGTGGT CCTGGAGGAG GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA 
GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG ACACTGGGCT CCGACGGGCG CTGTGGGCGC 
AGCGACGCTT TCAGCAGGTC ATCTTCCACC TCCAGCCAGG AGGAGAAGTC ATTCCATGCC 
GAGGAGTTGC CTCCTGGGGG CATCCCCATC GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC 
TCCGTGGACC TCGCCTTCCA GCCCTCTCAG CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA 
CTGCAGACCC TGCAGGACAT CCTGGGGGAC CCCGGGGACA AGGCTGAGGT TGGCCGGCTG 
AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA GGGATCCTGG ACGGGGAAGG TGCCGCCTGG 
TCAGCCCCGG GTGAGGAACG CCGGGGTCGG GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT 
TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG CGGCCCCGAG GCTACACCAT CTCGGATTCG 
GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG AGGGATGCCT TCAAGAACCG AGCAGGGACC 
TCCAACACCG AGAAGGTGCC AGGCATCAAC CCCAGCTTCG TGTTCCTGCA GCTCTACCAC 
TCACCGTTCT TTGGTGACGA GTCCAACAAG CCAATCCTTT TGCCTAACGA GTCTTTCGAA 
CGATCAGTGC AGCTCCTTGA TCAGATCCCG TCGTACGACA CGCACAAGAT CGCGGTCCTG 
TACGTGGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAACGA GCATGGCTCC 
TACAGGTACA CAGAGTTCCT CACGGGGCTG GGCAAGCTCA TCGAGCTCAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG CGGCCTGGAC GTGTGCGGCG AGGATGGCCA GTTCACCTAC 
TGCTGGCACG ACGACATCAT GCAAGCTGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG TGACAAGAAG CGCCACCTGG GCAACGACTT CGTGTCCATC 
GTCTACAACG ACTCTGGCGA GGACTTCAAG TTGGGCACCA TCAAGGGCCA ATTCAACTTC 
GTCCACGTGA TTATCACGCC CCTGGACTAC GAGTGCAACC TGGTGTCTCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGATACCAGC ATGGCCAAGA TCGTGTCTGA CCGAAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCCAATATGG CCTCGCAGGT GCACCATAGC 
CGCTCCAATC CCACCGACAT TTATCCCTCC AAGTGGATTG CCAGGCTCCG CCACATCAAG 
CGGCTGCGCC AGCGGATCCG TGAGGAAGCC CACTACTCAA GCGCCAGCCT GCCCCTGATG 
CAGACACACC CTCCTGGTCA TGCCAAGGCT CCGGCACAGG CCCCGGCCGA GTCCACGCCC 
ACCTATGAGA CGGGCCAGCG GAAACGCCTG GTCTCCTCTG TGGACGACTT CACTGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841209.1
CDS	663..5408
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985441.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985441.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGATT CTGCGGTGGT CCTGGAGGAG GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA 
GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG ACACTGGGCT CCGACGGGCG CTGTGGGCGC 
AGCGACGCTT TCAGCAGGTC ATCTTCCACC TCCAGCCAGG AGGAGAAGTC ATTCCATGCC 
GAGGAGTTGC CTCCTGGGGG CATCCCCATC GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC 
TCCGTGGACC TCGCCTTCCA GCCCTCTCAG CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA 
CTGCAGACCC TGCAGGACAT CCTGGGGGAC CCCGGGGACA AGGCTGAGGT TGGCCGGCTG 
AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA GGGATCCTGG ACGGGGAAGG TGCCGCCTGG 
TCAGCCCCGG GTGAGGAACG CCGGGGTCGG GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT 
TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG CGGCCCCGAG GCTACACCAT CTCGGATTCG 
GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG AGGGATGCCT TCAAGAACCG AGCAGGGACC 
TCCAACACCG AGAAGGTGCC AGGCATCAAC CCCAGCTTCG TGTTCCTGCA GCTCTACCAC 
TCACCGTTCT TTGGTGACGA GTCCAACAAG CCAATCCTTT TGCCTAACGA GTCTTTCGAA 
CGATCAGTGC AGCTCCTTGA TCAGATCCCG TCGTACGACA CGCACAAGAT CGCGGTCCTG 
TACGTGGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAACGA GCATGGCTCC 
TACAGGTACA CAGAGTTCCT CACGGGGCTG GGCAAGCTCA TCGAGCTCAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG CGGCCTGGAC GTGTGCGGCG AGGATGGCCA GTTCACCTAC 
TGCTGGCACG ACGACATCAT GCAAGCTGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG TGACAAGAAG CGCCACCTGG GCAACGACTT CGTGTCCATC 
GTCTACAACG ACTCTGGCGA GGACTTCAAG TTGGGCACCA TCAAGGGCCA ATTCAACTTC 
GTCCACGTGA TTATCACGCC CCTGGACTAC GAGTGCAACC TGGTGTCTCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGATACCAGC ATGGCCAAGA TCGTGTCTGA CCGAAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCCAATATGG CCTCGCAGGT GCACCATAGC 
CGCTCCAATC CCACCGACAT TTATCCCTCC AAGTGGATTG CCAGGCTCCG CCACATCAAG 
CGGCTGCGCC AGCGGATCCG TGAGGAAGCC CACTACTCAA GCGCCAGCCT GCCCCTGATG 
CAGACACACC CTCCTGGTCA TGCCAAGGCT CCGGCACAGG CCCCGGCCGA GTCCACGCCC 
ACCTATGAGA CGGGCCAGCG GAAACGCCTG GTCTCCTCTG TGGACGACTT CACTGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985439.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841207.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

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CloneID	OBa230695
Clone ID Related Accession (Same CDS sequence)	XM_024985441.1 , XM_024985439.1
Accession Version	XM_024985439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGATT CTGCGGTGGT CCTGGAGGAG GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA 
GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG ACACTGGGCT CCGACGGGCG CTGTGGGCGC 
AGCGACGCTT TCAGCAGGTC ATCTTCCACC TCCAGCCAGG AGGAGAAGTC ATTCCATGCC 
GAGGAGTTGC CTCCTGGGGG CATCCCCATC GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC 
TCCGTGGACC TCGCCTTCCA GCCCTCTCAG CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA 
CTGCAGACCC TGCAGGACAT CCTGGGGGAC CCCGGGGACA AGGCTGAGGT TGGCCGGCTG 
AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA GGGATCCTGG ACGGGGAAGG TGCCGCCTGG 
TCAGCCCCGG GTGAGGAACG CCGGGGTCGG GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT 
TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG CGGCCCCGAG GCTACACCAT CTCGGATTCG 
GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG AGGGATGCCT TCAAGAACCG AGCAGGGACC 
TCCAACACCG AGAAGGTGCC AGGCATCAAC CCCAGCTTCG TGTTCCTGCA GCTCTACCAC 
TCACCGTTCT TTGGTGACGA GTCCAACAAG CCAATCCTTT TGCCTAACGA GTCTTTCGAA 
CGATCAGTGC AGCTCCTTGA TCAGATCCCG TCGTACGACA CGCACAAGAT CGCGGTCCTG 
TACGTGGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAACGA GCATGGCTCC 
TACAGGTACA CAGAGTTCCT CACGGGGCTG GGCAAGCTCA TCGAGCTCAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG CGGCCTGGAC GTGTGCGGCG AGGATGGCCA GTTCACCTAC 
TGCTGGCACG ACGACATCAT GCAAGCTGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG TGACAAGAAG CGCCACCTGG GCAACGACTT CGTGTCCATC 
GTCTACAACG ACTCTGGCGA GGACTTCAAG TTGGGCACCA TCAAGGGCCA ATTCAACTTC 
GTCCACGTGA TTATCACGCC CCTGGACTAC GAGTGCAACC TGGTGTCTCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGATACCAGC ATGGCCAAGA TCGTGTCTGA CCGAAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCCAATATGG CCTCGCAGGT GCACCATAGC 
CGCTCCAATC CCACCGACAT TTATCCCTCC AAGTGGATTG CCAGGCTCCG CCACATCAAG 
CGGCTGCGCC AGCGGATCCG TGAGGAAGCC CACTACTCAA GCGCCAGCCT GCCCCTGATG 
CAGACACACC CTCCTGGTCA TGCCAAGGCT CCGGCACAGG CCCCGGCCGA GTCCACGCCC 
ACCTATGAGA CGGGCCAGCG GAAACGCCTG GTCTCCTCTG TGGACGACTT CACTGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841207.1
CDS	461..5206
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985439.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985439.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGATT CTGCGGTGGT CCTGGAGGAG GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA 
GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG ACACTGGGCT CCGACGGGCG CTGTGGGCGC 
AGCGACGCTT TCAGCAGGTC ATCTTCCACC TCCAGCCAGG AGGAGAAGTC ATTCCATGCC 
GAGGAGTTGC CTCCTGGGGG CATCCCCATC GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC 
TCCGTGGACC TCGCCTTCCA GCCCTCTCAG CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA 
CTGCAGACCC TGCAGGACAT CCTGGGGGAC CCCGGGGACA AGGCTGAGGT TGGCCGGCTG 
AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA GGGATCCTGG ACGGGGAAGG TGCCGCCTGG 
TCAGCCCCGG GTGAGGAACG CCGGGGTCGG GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT 
TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG CGGCCCCGAG GCTACACCAT CTCGGATTCG 
GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG AGGGATGCCT TCAAGAACCG AGCAGGGACC 
TCCAACACCG AGAAGGTGCC AGGCATCAAC CCCAGCTTCG TGTTCCTGCA GCTCTACCAC 
TCACCGTTCT TTGGTGACGA GTCCAACAAG CCAATCCTTT TGCCTAACGA GTCTTTCGAA 
CGATCAGTGC AGCTCCTTGA TCAGATCCCG TCGTACGACA CGCACAAGAT CGCGGTCCTG 
TACGTGGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAACGA GCATGGCTCC 
TACAGGTACA CAGAGTTCCT CACGGGGCTG GGCAAGCTCA TCGAGCTCAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG CGGCCTGGAC GTGTGCGGCG AGGATGGCCA GTTCACCTAC 
TGCTGGCACG ACGACATCAT GCAAGCTGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG TGACAAGAAG CGCCACCTGG GCAACGACTT CGTGTCCATC 
GTCTACAACG ACTCTGGCGA GGACTTCAAG TTGGGCACCA TCAAGGGCCA ATTCAACTTC 
GTCCACGTGA TTATCACGCC CCTGGACTAC GAGTGCAACC TGGTGTCTCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGATACCAGC ATGGCCAAGA TCGTGTCTGA CCGAAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCCAATATGG CCTCGCAGGT GCACCATAGC 
CGCTCCAATC CCACCGACAT TTATCCCTCC AAGTGGATTG CCAGGCTCCG CCACATCAAG 
CGGCTGCGCC AGCGGATCCG TGAGGAAGCC CACTACTCAA GCGCCAGCCT GCCCCTGATG 
CAGACACACC CTCCTGGTCA TGCCAAGGCT CCGGCACAGG CCCCGGCCGA GTCCACGCCC 
ACCTATGAGA CGGGCCAGCG GAAACGCCTG GTCTCCTCTG TGGACGACTT CACTGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024985440.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841208.1 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

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CloneID	OBa230696
Clone ID Related Accession (Same CDS sequence)	XM_024985440.1
Accession Version	XM_024985440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGATTCTG CGGTGGTCCT GGAGGAGGGA GGTCCAGGTG AGGCTGGTTT GTCTGCAGAG 
CCTCCCGAGT TGGAAGATTT TGAGGCGACA CTGGGCTCCG ACGGGCGCTG TGGGCGCAGC 
GACGCTTTCA GCAGGTCATC TTCCACCTCC AGCCAGGAGG AGAAGTCATT CCATGCCGAG 
GAGTTGCCTC CTGGGGGCAT CCCCATCGAG CGGGCGGTCT CGGAGGGCTC CCGGGCCTCC 
GTGGACCTCG CCTTCCAGCC CTCTCAGCCC CTGAGCAAGT CCAGCTCGTC GCCTGAACTG 
CAGACCCTGC AGGACATCCT GGGGGACCCC GGGGACAAGG CTGAGGTTGG CCGGCTGAGC 
CCCGAGGCCA AGGCCCGGTC GCAGTCAGGG ATCCTGGACG GGGAAGGTGC CGCCTGGTCA 
GCCCCGGGTG AGGAACGCCG GGGTCGGGGC CCTGCCCAGC CAGAGGGCCC CCTGCCTTCC 
AGCTGTCCTC GCTCCCCCAG CGGGCTGCGG CCCCGAGGCT ACACCATCTC GGATTCGGCC 
CCGTCACGCA GGGGCAAGAG GGTGGAGAGG GATGCCTTCA AGAACCGAGC AGGGACCTCC 
AACACCGAGA AGGTGCCAGG CATCAACCCC AGCTTCGTGT TCCTGCAGCT CTACCACTCA 
CCGTTCTTTG GTGACGAGTC CAACAAGCCA ATCCTTTTGC CTAACGAGTC TTTCGAACGA 
TCAGTGCAGC TCCTTGATCA GATCCCGTCG TACGACACGC ACAAGATCGC GGTCCTGTAC 
GTGGGAGAAG GCCAGAGCAA CAGCGAGCTC GCCATCCTGT CCAACGAGCA TGGCTCCTAC 
AGGTACACAG AGTTCCTCAC GGGGCTGGGC AAGCTCATCG AGCTCAAGGA CTGCCAGCCG 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGCGGCGAGG ATGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCTGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ACCGCTGTGA CAAGAAGCGC CACCTGGGCA ACGACTTCGT GTCCATCGTC 
TACAACGACT CTGGCGAGGA CTTCAAGTTG GGCACCATCA AGGGCCAATT CAACTTCGTC 
CACGTGATTA TCACGCCCCT GGACTACGAG TGCAACCTGG TGTCTCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA TACCAGCATG GCCAAGATCG TGTCTGACCG AAACCTGCCC 
TTCGTGGCCC GCCAGATGGC CCTGCACGCC AATATGGCCT CGCAGGTGCA CCATAGCCGC 
TCCAATCCCA CCGACATTTA TCCCTCCAAG TGGATTGCCA GGCTCCGCCA CATCAAGCGG 
CTGCGCCAGC GGATCCGTGA GGAAGCCCAC TACTCAAGCG CCAGCCTGCC CCTGATGCAG 
ACACACCCTC CTGGTCATGC CAAGGCTCCG GCACAGGCCC CGGCCGAGTC CACGCCCACC 
TATGAGACGG GCCAGCGGAA ACGCCTGGTC TCCTCTGTGG ACGACTTCAC TGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841208.1
CDS	461..5203
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_024985440.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985440.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCACA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGATTCTG CGGTGGTCCT GGAGGAGGGA GGTCCAGGTG AGGCTGGTTT GTCTGCAGAG 
CCTCCCGAGT TGGAAGATTT TGAGGCGACA CTGGGCTCCG ACGGGCGCTG TGGGCGCAGC 
GACGCTTTCA GCAGGTCATC TTCCACCTCC AGCCAGGAGG AGAAGTCATT CCATGCCGAG 
GAGTTGCCTC CTGGGGGCAT CCCCATCGAG CGGGCGGTCT CGGAGGGCTC CCGGGCCTCC 
GTGGACCTCG CCTTCCAGCC CTCTCAGCCC CTGAGCAAGT CCAGCTCGTC GCCTGAACTG 
CAGACCCTGC AGGACATCCT GGGGGACCCC GGGGACAAGG CTGAGGTTGG CCGGCTGAGC 
CCCGAGGCCA AGGCCCGGTC GCAGTCAGGG ATCCTGGACG GGGAAGGTGC CGCCTGGTCA 
GCCCCGGGTG AGGAACGCCG GGGTCGGGGC CCTGCCCAGC CAGAGGGCCC CCTGCCTTCC 
AGCTGTCCTC GCTCCCCCAG CGGGCTGCGG CCCCGAGGCT ACACCATCTC GGATTCGGCC 
CCGTCACGCA GGGGCAAGAG GGTGGAGAGG GATGCCTTCA AGAACCGAGC AGGGACCTCC 
AACACCGAGA AGGTGCCAGG CATCAACCCC AGCTTCGTGT TCCTGCAGCT CTACCACTCA 
CCGTTCTTTG GTGACGAGTC CAACAAGCCA ATCCTTTTGC CTAACGAGTC TTTCGAACGA 
TCAGTGCAGC TCCTTGATCA GATCCCGTCG TACGACACGC ACAAGATCGC GGTCCTGTAC 
GTGGGAGAAG GCCAGAGCAA CAGCGAGCTC GCCATCCTGT CCAACGAGCA TGGCTCCTAC 
AGGTACACAG AGTTCCTCAC GGGGCTGGGC AAGCTCATCG AGCTCAAGGA CTGCCAGCCG 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGCGGCGAGG ATGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCTGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ACCGCTGTGA CAAGAAGCGC CACCTGGGCA ACGACTTCGT GTCCATCGTC 
TACAACGACT CTGGCGAGGA CTTCAAGTTG GGCACCATCA AGGGCCAATT CAACTTCGTC 
CACGTGATTA TCACGCCCCT GGACTACGAG TGCAACCTGG TGTCTCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA TACCAGCATG GCCAAGATCG TGTCTGACCG AAACCTGCCC 
TTCGTGGCCC GCCAGATGGC CCTGCACGCC AATATGGCCT CGCAGGTGCA CCATAGCCGC 
TCCAATCCCA CCGACATTTA TCCCTCCAAG TGGATTGCCA GGCTCCGCCA CATCAAGCGG 
CTGCGCCAGC GGATCCGTGA GGAAGCCCAC TACTCAAGCG CCAGCCTGCC CCTGATGCAG 
ACACACCCTC CTGGTCATGC CAAGGCTCCG GCACAGGCCC CGGCCGAGTC CACGCCCACC 
TATGAGACGG GCCAGCGGAA ACGCCTGGTC TCCTCTGTGG ACGACTTCAC TGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460252.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315738.2 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

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CloneID	OBa230697
Clone ID Related Accession (Same CDS sequence)	XM_015460252.2
Accession Version	XM_015460252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACC TCTCTCACGA GCGCCAGCTT GGGGTCTGCA 
GACGAGAACT CGATGGCCCA GGCCGACGAC AACTTGAAAA ACCTCCACCT GGAGCTCACA 
GAGACGTGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACGGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACCGTGAC GACAAGCGTG GGGACCGGGA CCCGGTCACT GCTAGGCCTG 
GACTCTGGAG AGCTGCAGGG TGGCCCGGAG TTGAGCTCTG ACCCCAGCAT GCATGTGAGA 
CAGACAAAGG AGGCACCCGC CAAGCTGGAG TCCCAAGCTG GGCAGCAGGT GTACCACGGA 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCATGGCC TTCGCGTGGG TGCCTTGGAC 
GCTCCAGCCT GCTACTTCCC CAGCAGCCCC ACGTCCCCGG GCTCCCAGAC CGCTCCAGCC 
AGCCAGCCCG AGAAGGCCTC AGCTGGCAGC CCGCTCCCAG CACAGAAGGA GAAGACAAAC 
CTGGCCGCTT ACGTGCCCCT GCTGACCCAG GGCTGGGCAG AGATCCTGGT CCGCAGGCCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAGCA TGCCGCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGCG ACACAGCCCT GTACAAGTCG CTGTCGGTGC CGGCGGCCGG CTCAGCCAAG 
CCATCCCCGC CCCCACGCTC CAACACAGTG GCCTCTTTCT CCTCCCTGTG CCAGTCCAGG 
TGCCAAGGAA AGCTGCACAG GAGCATTTCC TGGGCAGATT CTGCGGTGGT CCTGGAGGAG 
GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG 
ACACTGGGCT CCGACGGGCG CTGTGGGCGC AGCGACGCTT TCAGCAGGTC ATCTTCCACC 
TCCAGCCAGG AGGAGAAGTC ATTCCATGCC GAGGAGTTGC CTCCTGGGGG CATCCCCATC 
GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC TCCGTGGACC TCGCCTTCCA GCCCTCTCAG 
CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA CTGCAGACCC TGCAGGACAT CCTGGGGGAC 
CCCGGGGACA AGGCTGAGGT TGGCCGGCTG AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ACGGGGAAGG TGCCGCCTGG TCAGCCCCGG GTGAGGAACG CCGGGGTCGG 
GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG 
CGGCCCCGAG GCTACACCAT CTCGGATTCG GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG 
AGGGATGCCT TCAAGAACCG AGCAGGGACC TCCAACACCG AGAAGGTGCC AGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCACCGTTCT TTGGTGACGA GTCCAACAAG 
CCAATCCTTT TGCCTAACGA GTCTTTCGAA CGATCAGTGC AGCTCCTTGA TCAGATCCCG 
TCGTACGACA CGCACAAGAT CGCGGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGCGAG 
CTCGCCATCC TGTCCAACGA GCATGGCTCC TACAGGTACA CAGAGTTCCT CACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG CGGCCTGGAC 
GTGTGCGGCG AGGATGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCTGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAACGACTT CGTGTCCATC GTCTACAACG ACTCTGGCGA GGACTTCAAG 
TTGGGCACCA TCAAGGGCCA ATTCAACTTC GTCCACGTGA TTATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCTCT GCAGTGCAGG AAAGACATGG AGGGCCTTGT GGATACCAGC 
ATGGCCAAGA TCGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC 
GCCAATATGG CCTCGCAGGT GCACCATAGC CGCTCCAATC CCACCGACAT TTATCCCTCC 
AAGTGGATTG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AGCGGATCCG TGAGGAAGCC 
CACTACTCAA GCGCCAGCCT GCCCCTGATG CAGACACACC CTCCTGGTCA TGCCAAGGCT 
CCGGCACAGG CCCCGGCCGA GTCCACGCCC ACCTATGAGA CGGGCCAGCG GAAACGCCTG 
GTCTCCTCTG TGGACGACTT CACTGAGTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315738.2
CDS	139..5454
Translation	MAKPASKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460252.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460252.2

ORF Insert Sequence:

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACC TCTCTCACGA GCGCCAGCTT GGGGTCTGCA 
GACGAGAACT CGATGGCCCA GGCCGACGAC AACTTGAAAA ACCTCCACCT GGAGCTCACA 
GAGACGTGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACGGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACCGTGAC GACAAGCGTG GGGACCGGGA CCCGGTCACT GCTAGGCCTG 
GACTCTGGAG AGCTGCAGGG TGGCCCGGAG TTGAGCTCTG ACCCCAGCAT GCATGTGAGA 
CAGACAAAGG AGGCACCCGC CAAGCTGGAG TCCCAAGCTG GGCAGCAGGT GTACCACGGA 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCATGGCC TTCGCGTGGG TGCCTTGGAC 
GCTCCAGCCT GCTACTTCCC CAGCAGCCCC ACGTCCCCGG GCTCCCAGAC CGCTCCAGCC 
AGCCAGCCCG AGAAGGCCTC AGCTGGCAGC CCGCTCCCAG CACAGAAGGA GAAGACAAAC 
CTGGCCGCTT ACGTGCCCCT GCTGACCCAG GGCTGGGCAG AGATCCTGGT CCGCAGGCCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAGCA TGCCGCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGCG ACACAGCCCT GTACAAGTCG CTGTCGGTGC CGGCGGCCGG CTCAGCCAAG 
CCATCCCCGC CCCCACGCTC CAACACAGTG GCCTCTTTCT CCTCCCTGTG CCAGTCCAGG 
TGCCAAGGAA AGCTGCACAG GAGCATTTCC TGGGCAGATT CTGCGGTGGT CCTGGAGGAG 
GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG 
ACACTGGGCT CCGACGGGCG CTGTGGGCGC AGCGACGCTT TCAGCAGGTC ATCTTCCACC 
TCCAGCCAGG AGGAGAAGTC ATTCCATGCC GAGGAGTTGC CTCCTGGGGG CATCCCCATC 
GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC TCCGTGGACC TCGCCTTCCA GCCCTCTCAG 
CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA CTGCAGACCC TGCAGGACAT CCTGGGGGAC 
CCCGGGGACA AGGCTGAGGT TGGCCGGCTG AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA 
GGGATCCTGG ACGGGGAAGG TGCCGCCTGG TCAGCCCCGG GTGAGGAACG CCGGGGTCGG 
GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG 
CGGCCCCGAG GCTACACCAT CTCGGATTCG GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG 
AGGGATGCCT TCAAGAACCG AGCAGGGACC TCCAACACCG AGAAGGTGCC AGGCATCAAC 
CCCAGCTTCG TGTTCCTGCA GCTCTACCAC TCACCGTTCT TTGGTGACGA GTCCAACAAG 
CCAATCCTTT TGCCTAACGA GTCTTTCGAA CGATCAGTGC AGCTCCTTGA TCAGATCCCG 
TCGTACGACA CGCACAAGAT CGCGGTCCTG TACGTGGGAG AAGGCCAGAG CAACAGCGAG 
CTCGCCATCC TGTCCAACGA GCATGGCTCC TACAGGTACA CAGAGTTCCT CACGGGGCTG 
GGCAAGCTCA TCGAGCTCAA GGACTGCCAG CCGGACAAGG TGTACCTGGG CGGCCTGGAC 
GTGTGCGGCG AGGATGGCCA GTTCACCTAC TGCTGGCACG ACGACATCAT GCAAGCTGTC 
TTCCACATCG CCACCCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGCCACCTGG GCAACGACTT CGTGTCCATC GTCTACAACG ACTCTGGCGA GGACTTCAAG 
TTGGGCACCA TCAAGGGCCA ATTCAACTTC GTCCACGTGA TTATCACGCC CCTGGACTAC 
GAGTGCAACC TGGTGTCTCT GCAGTGCAGG AAAGACATGG AGGGCCTTGT GGATACCAGC 
ATGGCCAAGA TCGTGTCTGA CCGAAACCTG CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC 
GCCAATATGG CCTCGCAGGT GCACCATAGC CGCTCCAATC CCACCGACAT TTATCCCTCC 
AAGTGGATTG CCAGGCTCCG CCACATCAAG CGGCTGCGCC AGCGGATCCG TGAGGAAGCC 
CACTACTCAA GCGCCAGCCT GCCCCTGATG CAGACACACC CTCCTGGTCA TGCCAAGGCT 
CCGGCACAGG CCCCGGCCGA GTCCACGCCC ACCTATGAGA CGGGCCAGCG GAAACGCCTG 
GTCTCCTCTG TGGACGACTT CACTGAGTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460255.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315741.2 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.

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CloneID	OBa230698
Clone ID Related Accession (Same CDS sequence)	XM_015460255.2
Accession Version	XM_015460255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACC TCTCTCACGA GCGCCAGCTT GGGGTCTGCA 
GACGAGAACT CGATGGCCCA GGCCGACGAC AACTTGAAAA ACCTCCACCT GGAGCTCACA 
GAGACGTGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACGGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACCGTGAC GACAAGCGTG GGGACCGGGA CCCGGTCACT GCTAGGCCTG 
GACTCTGGAG AGCTGCAGGG TGGCCCGGAG TTGAGCTCTG ACCCCAGCAT GCATGTGAGA 
CAGACAAAGG AGGCACCCGC CAAGCTGGAG TCCCAAGCTG GGCAGCAGGT GTACCACGGA 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCATGGCC TTCGCGTGGG TGCCTTGGAC 
GCTCCAGCCT GCTACTTCCC CAGCAGCCCC ACGTCCCCGG GCTCCCAGAC CGCTCCAGCC 
AGCCAGCCCG AGAAGGCCTC AGCTGGCAGC CCGCTCCCAG CACAGAAGGA GAAGACAAAC 
CTGGCCGCTT ACGTGCCCCT GCTGACCCAG GGCTGGGCAG AGATCCTGGT CCGCAGGCCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAGCA TGCCGCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGCG ACACAGCCCT GTACAAGTCG CTGTCGGTGC CGGCGGCCGG CTCAGCCAAG 
CCATCCCCGC CCCCACGCTC CAACACAGAT TCTGCGGTGG TCCTGGAGGA GGGAGGTCCA 
GGTGAGGCTG GTTTGTCTGC AGAGCCTCCC GAGTTGGAAG ATTTTGAGGC GACACTGGGC 
TCCGACGGGC GCTGTGGGCG CAGCGACGCT TTCAGCAGGT CATCTTCCAC CTCCAGCCAG 
GAGGAGAAGT CATTCCATGC CGAGGAGTTG CCTCCTGGGG GCATCCCCAT CGAGCGGGCG 
GTCTCGGAGG GCTCCCGGGC CTCCGTGGAC CTCGCCTTCC AGCCCTCTCA GCCCCTGAGC 
AAGTCCAGCT CGTCGCCTGA ACTGCAGACC CTGCAGGACA TCCTGGGGGA CCCCGGGGAC 
AAGGCTGAGG TTGGCCGGCT GAGCCCCGAG GCCAAGGCCC GGTCGCAGTC AGGGATCCTG 
GACGGGGAAG GTGCCGCCTG GTCAGCCCCG GGTGAGGAAC GCCGGGGTCG GGGCCCTGCC 
CAGCCAGAGG GCCCCCTGCC TTCCAGCTGT CCTCGCTCCC CCAGCGGGCT GCGGCCCCGA 
GGCTACACCA TCTCGGATTC GGCCCCGTCA CGCAGGGGCA AGAGGGTGGA GAGGGATGCC 
TTCAAGAACC GAGCAGGGAC CTCCAACACC GAGAAGGTGC CAGGCATCAA CCCCAGCTTC 
GTGTTCCTGC AGCTCTACCA CTCACCGTTC TTTGGTGACG AGTCCAACAA GCCAATCCTT 
TTGCCTAACG AGTCTTTCGA ACGATCAGTG CAGCTCCTTG ATCAGATCCC GTCGTACGAC 
ACGCACAAGA TCGCGGTCCT GTACGTGGGA GAAGGCCAGA GCAACAGCGA GCTCGCCATC 
CTGTCCAACG AGCATGGCTC CTACAGGTAC ACAGAGTTCC TCACGGGGCT GGGCAAGCTC 
ATCGAGCTCA AGGACTGCCA GCCGGACAAG GTGTACCTGG GCGGCCTGGA CGTGTGCGGC 
GAGGATGGCC AGTTCACCTA CTGCTGGCAC GACGACATCA TGCAAGCTGT CTTCCACATC 
GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAACGACT TCGTGTCCAT CGTCTACAAC GACTCTGGCG AGGACTTCAA GTTGGGCACC 
ATCAAGGGCC AATTCAACTT CGTCCACGTG ATTATCACGC CCCTGGACTA CGAGTGCAAC 
CTGGTGTCTC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGATACCAG CATGGCCAAG 
ATCGTGTCTG ACCGAAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA CGCCAATATG 
GCCTCGCAGG TGCACCATAG CCGCTCCAAT CCCACCGACA TTTATCCCTC CAAGTGGATT 
GCCAGGCTCC GCCACATCAA GCGGCTGCGC CAGCGGATCC GTGAGGAAGC CCACTACTCA 
AGCGCCAGCC TGCCCCTGAT GCAGACACAC CCTCCTGGTC ATGCCAAGGC TCCGGCACAG 
GCCCCGGCCG AGTCCACGCC CACCTATGAG ACGGGCCAGC GGAAACGCCT GGTCTCCTCT 
GTGGACGACT TCACTGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315741.2
CDS	139..5385
Translation	MAKPASKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460255.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460255.2

ORF Insert Sequence:

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGCAG GATCCAGACC TCTCTCACGA GCGCCAGCTT GGGGTCTGCA 
GACGAGAACT CGATGGCCCA GGCCGACGAC AACTTGAAAA ACCTCCACCT GGAGCTCACA 
GAGACGTGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACGGC GGTCCCCAAG 
AGGTCCCCCG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACCGTGAC GACAAGCGTG GGGACCGGGA CCCGGTCACT GCTAGGCCTG 
GACTCTGGAG AGCTGCAGGG TGGCCCGGAG TTGAGCTCTG ACCCCAGCAT GCATGTGAGA 
CAGACAAAGG AGGCACCCGC CAAGCTGGAG TCCCAAGCTG GGCAGCAGGT GTACCACGGA 
GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCATGGCC TTCGCGTGGG TGCCTTGGAC 
GCTCCAGCCT GCTACTTCCC CAGCAGCCCC ACGTCCCCGG GCTCCCAGAC CGCTCCAGCC 
AGCCAGCCCG AGAAGGCCTC AGCTGGCAGC CCGCTCCCAG CACAGAAGGA GAAGACAAAC 
CTGGCCGCTT ACGTGCCCCT GCTGACCCAG GGCTGGGCAG AGATCCTGGT CCGCAGGCCC 
ACAGGGAACA CCAGCTGGCT GATGAGCCTG GAGAACCCGC TCAGCCCCTT CTCCTCGGAC 
ATCAACAGCA TGCCGCTGCA GGAGCTGTCC AATGCCCTCA TGGCCGCCGA GCGCTTCAAG 
GAGCGCCGCG ACACAGCCCT GTACAAGTCG CTGTCGGTGC CGGCGGCCGG CTCAGCCAAG 
CCATCCCCGC CCCCACGCTC CAACACAGAT TCTGCGGTGG TCCTGGAGGA GGGAGGTCCA 
GGTGAGGCTG GTTTGTCTGC AGAGCCTCCC GAGTTGGAAG ATTTTGAGGC GACACTGGGC 
TCCGACGGGC GCTGTGGGCG CAGCGACGCT TTCAGCAGGT CATCTTCCAC CTCCAGCCAG 
GAGGAGAAGT CATTCCATGC CGAGGAGTTG CCTCCTGGGG GCATCCCCAT CGAGCGGGCG 
GTCTCGGAGG GCTCCCGGGC CTCCGTGGAC CTCGCCTTCC AGCCCTCTCA GCCCCTGAGC 
AAGTCCAGCT CGTCGCCTGA ACTGCAGACC CTGCAGGACA TCCTGGGGGA CCCCGGGGAC 
AAGGCTGAGG TTGGCCGGCT GAGCCCCGAG GCCAAGGCCC GGTCGCAGTC AGGGATCCTG 
GACGGGGAAG GTGCCGCCTG GTCAGCCCCG GGTGAGGAAC GCCGGGGTCG GGGCCCTGCC 
CAGCCAGAGG GCCCCCTGCC TTCCAGCTGT CCTCGCTCCC CCAGCGGGCT GCGGCCCCGA 
GGCTACACCA TCTCGGATTC GGCCCCGTCA CGCAGGGGCA AGAGGGTGGA GAGGGATGCC 
TTCAAGAACC GAGCAGGGAC CTCCAACACC GAGAAGGTGC CAGGCATCAA CCCCAGCTTC 
GTGTTCCTGC AGCTCTACCA CTCACCGTTC TTTGGTGACG AGTCCAACAA GCCAATCCTT 
TTGCCTAACG AGTCTTTCGA ACGATCAGTG CAGCTCCTTG ATCAGATCCC GTCGTACGAC 
ACGCACAAGA TCGCGGTCCT GTACGTGGGA GAAGGCCAGA GCAACAGCGA GCTCGCCATC 
CTGTCCAACG AGCATGGCTC CTACAGGTAC ACAGAGTTCC TCACGGGGCT GGGCAAGCTC 
ATCGAGCTCA AGGACTGCCA GCCGGACAAG GTGTACCTGG GCGGCCTGGA CGTGTGCGGC 
GAGGATGGCC AGTTCACCTA CTGCTGGCAC GACGACATCA TGCAAGCTGT CTTCCACATC 
GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGCCACCTG 
GGCAACGACT TCGTGTCCAT CGTCTACAAC GACTCTGGCG AGGACTTCAA GTTGGGCACC 
ATCAAGGGCC AATTCAACTT CGTCCACGTG ATTATCACGC CCCTGGACTA CGAGTGCAAC 
CTGGTGTCTC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGATACCAG CATGGCCAAG 
ATCGTGTCTG ACCGAAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA CGCCAATATG 
GCCTCGCAGG TGCACCATAG CCGCTCCAAT CCCACCGACA TTTATCCCTC CAAGTGGATT 
GCCAGGCTCC GCCACATCAA GCGGCTGCGC CAGCGGATCC GTGAGGAAGC CCACTACTCA 
AGCGCCAGCC TGCCCCTGAT GCAGACACAC CCTCCTGGTC ATGCCAAGGC TCCGGCACAG 
GCCCCGGCCG AGTCCACGCC CACCTATGAG ACGGGCCAGC GGAAACGCCT GGTCTCCTCT 
GTGGACGACT TCACTGAGTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460256.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315742.2 Bos taurus TSC complex subunit 2 (TSC2), transcript variant X14, mRNA.

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CloneID	OBa230699
Clone ID Related Accession (Same CDS sequence)	XM_015460256.2
Accession Version	XM_015460256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tuberin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACCTCT CTCACGAGCG CCAGCTTGGG GTCTGCAGAC 
GAGAACTCGA TGGCCCAGGC CGACGACAAC TTGAAAAACC TCCACCTGGA GCTCACAGAG 
ACGTGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACGGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACCC GGTCACTGCT AGGCCTGGAC 
TCTGGAGAGC TGCAGGGTGG CCCGGAGTTG AGCTCTGACC CCAGCATGCA TGTGAGACAG 
ACAAAGGAGG CACCCGCCAA GCTGGAGTCC CAAGCTGGGC AGCAGGTGTA CCACGGAGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CATGGCCTTC GCGTGGGTGC CTTGGACGCT 
CCAGCCTGCT ACTTCCCCAG CAGCCCCACG TCCCCGGGCT CCCAGACCGC TCCAGCCAGC 
CAGCCCGAGA AGGCCTCAGC TGGCAGCCCG CTCCCAGCAC AGAAGGAGAA GACAAACCTG 
GCCGCTTACG TGCCCCTGCT GACCCAGGGC TGGGCAGAGA TCCTGGTCCG CAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAGCATGC CGCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGCGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCGG CGGCCGGCTC AGCCAAGCCA 
TCCCCGCCCC CACGCTCCAA CACAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315742.2
CDS	163..5406
Translation	MAKPASKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460256.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TSC complex subunit 2 (TSC2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460256.2

ORF Insert Sequence:

ATGGCTAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAGA AGTTCAAGAT TCTTTTGGGA 
CTGGGGACTC CACGGCCAAA TCCCAGGTCT GCAGAGGGCA AACAGACTGA GTTTATCATC 
ACCGCGGAAA TCCTGAGAGA GCTGAGTGTT GAATGTGGCC TCAATAATCG CATCCGGGTG 
ATAGGGCAGA TCTGTGAGGT TGCCAGAACG AAGAAATTCG AAGAGCACGC GGTGGAAGCA 
CTCTGGAAGG CCGTCTCGGA CCTGCTGCAG CCAGAGCGGC CACCCGAGGC CCGGCATGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCAGG 
GCCCTCTTCT TCAAGGTCAT CAAGGATTAT CCCTCTAATG AAGACCTCCA CGAGAGACTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA AGAAGAGCTG 
GCGGACTTTG TCCTGCAGTG GATGGACGTT GGCCTGTCCT CCGAATTCCT CCTGGTGCTC 
GTGAACTTAG TCAAATTCAA CAGCTGTTAC CTTGATGAGT ACATCGCATC CATGGTCCAC 
ATGGTCTGCC TGCTGTGTGT CCAGACCGTG TCCTCCGTGG ACATAGAGGT GTCTCTGCAG 
GTGCTGGATG CTGTGGTCTG CTACAACTGT CTGCCAGCCG AGAGCCTCCC CCTATTCATC 
GTCACTCTCT GCCGCACCAT CAACGTCAAG GAGCTCTGTG AACCTTGCTG GAAGCTGATG 
CGCAACCTCC TGGGCACCCA CCTGGGCCAC AGCGCCATCT ACCACATGTG CCGCATCATG 
GAAGACAGAG CCTACATGGA GGACGCACCA CTGCTGAGAG GAGCCGTGTT CTTTGTTGGC 
ATGGCTCTCT GGGGTGCCCA CCGGCTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCGTTTT ATGAGGCCAT GACCTGTCCG AATGAGGTGG TGTCCTACGA GATTGTCCTC 
TCCATAACCA GACTCATCAA GAAGTACAGG AGGGAGCTCC AGGCCGTGAC CTGGGACATC 
CTGCTGAACA TCATCGAGCG CCTGCTCCAG CAGCTCCAGA GCCTGGACAG CCCAGAGCTG 
AGCGCCATCG TGCACGATCT GCTGAGCACA GTGGAGGAGC TGTGTGACCA GAACGAGTTT 
CACGGCTCCC AGGAGAGATA CTTTGAACTC GTGGAGAGGT GTGCGGACCA GAGGCCTGAG 
TCATCCCTTT TAAACTTAAT AACCTACAGG GCTCAGTCCA TCCATCCAGC GAAGGACGGC 
TGGATCCACA ACCTGCAGCT GCTGATGGAG AGGTTTTTCA GGAACGAGTC ACGCAGTGCC 
GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAC 
GAGGAGGAGC TTATCAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC TGAGGATAGA 
GACCACCAGG TCCGGAAGCT GGCCACTCAG CTGCTGGTAG ATCTGGCCGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGACATT GTTGAGAAGG TGATCGCCCG TTCCCTCTCT 
CCGCCCCCCG AGCTGGAGGA GAGGGATGTG GCCGCGTACT CGGCCTCCTT GGAGGATGTG 
AAGACTGCGG TCCTGGGGCT CCTGGTCATC CTTCAGACTA AGCTGTATGC CCTGCCTGCC 
AGCCATGCCA CACGCGTGTA CGAGACGCTG GTCAGCCACA TTCAGCTCCA CTACAGGCAC 
AGCTACACCC TGCCCATCGC CAGCAGCATC CGTCTGCAGG CCTTCGACTT CCTGCTGCAG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAGCA AAGACGGGCT TGTGAGGTTC 
AGCCCCTACT GCGTCTGTGA CTGCCTGGAG ACAGAGAGGA GCTCCGAGAA GAAGGCCGGG 
TGCCCCCTGT CCCCGCCCGC CGGGCCCCCT GGCCCTGCGC CTGCGGGCCC TGCCGTGCGC 
CTTGGGTCCC TGCCCTACTC CCTGCTCTTC CGTGTTCTGC TGCAGTGTCT GAAGCAGGAG 
ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTGC CCGAGTCGCT GCGCTATAAG 
GTCCTCATCT TCACCTCCCC ATGCAGTGTC GACCAGCTGT CCGCCGCCCT CTGCTCCATG 
CTTTCAGGTC CCAAGACCCT GGAGCGGCTC CGAGGCACTC CAGAAGGCTT CTCCAGAACC 
GACCTGCATC TCGCTGTGGT TCCCGTGCTG ACAGCCCTGA TCTCTTACCA TAACTACTTG 
GACAAAACTA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTGGCCGTG TGCAGCGTGG AGATGCCCGA CGTCATCCTC 
AAGGCACTGC CCGTCCTGGT TGTGAAGCTC ACACACATCT CGGCCACGGC CAGCATGGCC 
ATCCCGCTCC TCGAGTTCCT GTCCACTCTG GCCAGACTGC CTCACCTCTA CAGGAACTTC 
GCAGCAGAGC AGTATGCCAG TGTGTTTGCC ATCTCCCTGC CGTATACCAA CCCCTCCAAG 
TTCAATCAGT ACATCGTGTG CCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA CTTTGTTCCT TACATCACCA AGGGTCTGCG CTCCAACGTC 
CTCCTGTCTT TCGATGACAC CCCCGAGAAG GACAGCTTCC GAGCGCGGAG CACCAGTCTC 
AACGAGAGGC CCAAGAGGAT CCAGACCTCT CTCACGAGCG CCAGCTTGGG GTCTGCAGAC 
GAGAACTCGA TGGCCCAGGC CGACGACAAC TTGAAAAACC TCCACCTGGA GCTCACAGAG 
ACGTGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACGGCGGT CCCCAAGAGG 
TCCCCCGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CCGTGACGAC AAGCGTGGGG ACCGGGACCC GGTCACTGCT AGGCCTGGAC 
TCTGGAGAGC TGCAGGGTGG CCCGGAGTTG AGCTCTGACC CCAGCATGCA TGTGAGACAG 
ACAAAGGAGG CACCCGCCAA GCTGGAGTCC CAAGCTGGGC AGCAGGTGTA CCACGGAGCC 
CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CATGGCCTTC GCGTGGGTGC CTTGGACGCT 
CCAGCCTGCT ACTTCCCCAG CAGCCCCACG TCCCCGGGCT CCCAGACCGC TCCAGCCAGC 
CAGCCCGAGA AGGCCTCAGC TGGCAGCCCG CTCCCAGCAC AGAAGGAGAA GACAAACCTG 
GCCGCTTACG TGCCCCTGCT GACCCAGGGC TGGGCAGAGA TCCTGGTCCG CAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC CTCGGACATC 
AACAGCATGC CGCTGCAGGA GCTGTCCAAT GCCCTCATGG CCGCCGAGCG CTTCAAGGAG 
CGCCGCGACA CAGCCCTGTA CAAGTCGCTG TCGGTGCCGG CGGCCGGCTC AGCCAAGCCA 
TCCCCGCCCC CACGCTCCAA CACAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_010825645.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823947.2 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa88433
Clone ID Related Accession (Same CDS sequence)	XM_010825645.2 , XM_015469258.1 , XM_010819198.2 , XM_015460254.1 , XM_015460257.1 , XM_003584007.4
Accession Version	XM_010825645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823947.2
CDS	463..5406
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_010825645.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825645.2

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015469258.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324744.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.

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CloneID	OBa88433
Clone ID Related Accession (Same CDS sequence)	XM_010825645.2 , XM_015469258.1 , XM_010819198.2 , XM_015460254.1 , XM_015460257.1 , XM_003584007.4
Accession Version	XM_015469258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324744.1
CDS	295..5238
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015469258.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469258.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_010819198.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817500.2 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OBa88433
Clone ID Related Accession (Same CDS sequence)	XM_010825645.2 , XM_015469258.1 , XM_010819198.2 , XM_015460254.1 , XM_015460257.1 , XM_003584007.4
Accession Version	XM_010819198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817500.2
CDS	650..5593
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_010819198.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819198.2

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460254.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315740.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OBa88433
Clone ID Related Accession (Same CDS sequence)	XM_010825645.2 , XM_015469258.1 , XM_010819198.2 , XM_015460254.1 , XM_015460257.1 , XM_003584007.4
Accession Version	XM_015460254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315740.1
CDS	463..5406
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460254.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460254.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460257.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315743.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.

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CloneID	OBa88433
Clone ID Related Accession (Same CDS sequence)	XM_010825645.2 , XM_015469258.1 , XM_010819198.2 , XM_015460254.1 , XM_015460257.1 , XM_003584007.4
Accession Version	XM_015460257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315743.1
CDS	295..5238
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460257.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460257.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003584007.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003584055.2 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OBa88433
Clone ID Related Accession (Same CDS sequence)	XM_010825645.2 , XM_015469258.1 , XM_010819198.2 , XM_015460254.1 , XM_015460257.1 , XM_003584007.4
Accession Version	XM_003584007.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003584007.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003584055.2
CDS	650..5593
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003584007.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003584007.4

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTGCC AGTCCAGGTG CCAAGGAAAG 
CTGCACAGGA GCATTTCCTG GGCAGATTCT GCGGTGGTCC TGGAGGAGGG AGGTCCAGGT 
GAGGCTGGTT TGTCTGCAGA GCCTCCCGAG TTGGAAGATT TTGAGGCGAC ACTGGGCTCC 
GACGGGCGCT GTGGGCGCAG CGACGCTTTC AGCAGGTCAT CTTCCACCTC CAGCCAGGAG 
GAGAAGTCAT TCCATGCCGA GGAGTTGCCT CCTGGGGGCA TCCCCATCGA GCGGGCGGTC 
TCGGAGGGCT CCCGGGCCTC CGTGGACCTC GCCTTCCAGC CCTCTCAGCC CCTGAGCAAG 
TCCAGCTCGT CGCCTGAACT GCAGACCCTG CAGGACATCC TGGGGGACCC CGGGGACAAG 
GCTGAGGTTG GCCGGCTGAG CCCCGAGGCC AAGGCCCGGT CGCAGTCAGG GATCCTGGAC 
GGGGAAGGTG CCGCCTGGTC AGCCCCGGGT GAGGAACGCC GGGGTCGGGG CCCTGCCCAG 
CCAGAGGGCC CCCTGCCTTC CAGCTGTCCT CGCTCCCCCA GCGGGCTGCG GCCCCGAGGC 
TACACCATCT CGGATTCGGC CCCGTCACGC AGGGGCAAGA GGGTGGAGAG GGATGCCTTC 
AAGAACCGAG CAGGGACCTC CAACACCGAG AAGGTGCCAG GCATCAACCC CAGCTTCGTG 
TTCCTGCAGC TCTACCACTC ACCGTTCTTT GGTGACGAGT CCAACAAGCC AATCCTTTTG 
CCTAACGAGT CTTTCGAACG ATCAGTGCAG CTCCTTGATC AGATCCCGTC GTACGACACG 
CACAAGATCG CGGTCCTGTA CGTGGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAACGAGC ATGGCTCCTA CAGGTACACA GAGTTCCTCA CGGGGCTGGG CAAGCTCATC 
GAGCTCAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGCG GCCTGGACGT GTGCGGCGAG 
GATGGCCAGT TCACCTACTG CTGGCACGAC GACATCATGC AAGCTGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC 
AACGACTTCG TGTCCATCGT CTACAACGAC TCTGGCGAGG ACTTCAAGTT GGGCACCATC 
AAGGGCCAAT TCAACTTCGT CCACGTGATT ATCACGCCCC TGGACTACGA GTGCAACCTG 
GTGTCTCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ATACCAGCAT GGCCAAGATC 
GTGTCTGACC GAAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC CAATATGGCC 
TCGCAGGTGC ACCATAGCCG CTCCAATCCC ACCGACATTT ATCCCTCCAA GTGGATTGCC 
AGGCTCCGCC ACATCAAGCG GCTGCGCCAG CGGATCCGTG AGGAAGCCCA CTACTCAAGC 
GCCAGCCTGC CCCTGATGCA GACACACCCT CCTGGTCATG CCAAGGCTCC GGCACAGGCC 
CCGGCCGAGT CCACGCCCAC CTATGAGACG GGCCAGCGGA AACGCCTGGT CTCCTCTGTG 
GACGACTTCA CTGAGTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460250.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315736.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa88434
Clone ID Related Accession (Same CDS sequence)	XM_015460250.1 , XM_015469255.1
Accession Version	XM_015460250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGGATCC AGACCTCTCT CACGAGCGCC AGCTTGGGGT CTGCAGACGA GAACTCGATG 
GCCCAGGCCG ACGACAACTT GAAAAACCTC CACCTGGAGC TCACAGAGAC GTGTCTGGAC 
ATGATGGCCA GATATGTGTT CTCCAACTTC ACGGCGGTCC CCAAGAGGTC CCCCGTGGGA 
GAGTTCCTCC TGGCTGGTGG CAGGACCAAA ACCTGGCTGG TTGGGAACAA GCTTGTCACC 
GTGACGACAA GCGTGGGGAC CGGGACCCGG TCACTGCTAG GCCTGGACTC TGGAGAGCTG 
CAGGGTGGCC CGGAGTTGAG CTCTGACCCC AGCATGCATG TGAGACAGAC AAAGGAGGCA 
CCCGCCAAGC TGGAGTCCCA AGCTGGGCAG CAGGTGTACC ACGGAGCCCG GGACCGGGTC 
CGCTCCATGT CCGGGGGCCA TGGCCTTCGC GTGGGTGCCT TGGACGCTCC AGCCTGCTAC 
TTCCCCAGCA GCCCCACGTC CCCGGGCTCC CAGACCGCTC CAGCCAGCCA GCCCGAGAAG 
GCCTCAGCTG GCAGCCCGCT CCCAGCACAG AAGGAGAAGA CAAACCTGGC CGCTTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCAGAGATC CTGGTCCGCA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCCT CGGACATCAA CAGCATGCCG 
CTGCAGGAGC TGTCCAATGC CCTCATGGCC GCCGAGCGCT TCAAGGAGCG CCGCGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCGGCG GCCGGCTCAG CCAAGCCATC CCCGCCCCCA 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTGCCAGT CCAGGTGCCA AGGAAAGCTG 
CACAGGAGCA TTTCCTGGGC AGATTCTGCG GTGGTCCTGG AGGAGGGAGG TCCAGGTGAG 
GCTGGTTTGT CTGCAGAGCC TCCCGAGTTG GAAGATTTTG AGGCGACACT GGGCTCCGAC 
GGGCGCTGTG GGCGCAGCGA CGCTTTCAGC AGGTCATCTT CCACCTCCAG CCAGGAGGAG 
AAGTCATTCC ATGCCGAGGA GTTGCCTCCT GGGGGCATCC CCATCGAGCG GGCGGTCTCG 
GAGGGCTCCC GGGCCTCCGT GGACCTCGCC TTCCAGCCCT CTCAGCCCCT GAGCAAGTCC 
AGCTCGTCGC CTGAACTGCA GACCCTGCAG GACATCCTGG GGGACCCCGG GGACAAGGCT 
GAGGTTGGCC GGCTGAGCCC CGAGGCCAAG GCCCGGTCGC AGTCAGGGAT CCTGGACGGG 
GAAGGTGCCG CCTGGTCAGC CCCGGGTGAG GAACGCCGGG GTCGGGGCCC TGCCCAGCCA 
GAGGGCCCCC TGCCTTCCAG CTGTCCTCGC TCCCCCAGCG GGCTGCGGCC CCGAGGCTAC 
ACCATCTCGG ATTCGGCCCC GTCACGCAGG GGCAAGAGGG TGGAGAGGGA TGCCTTCAAG 
AACCGAGCAG GGACCTCCAA CACCGAGAAG GTGCCAGGCA TCAACCCCAG CTTCGTGTTC 
CTGCAGCTCT ACCACTCACC GTTCTTTGGT GACGAGTCCA ACAAGCCAAT CCTTTTGCCT 
AACGAGTCTT TCGAACGATC AGTGCAGCTC CTTGATCAGA TCCCGTCGTA CGACACGCAC 
AAGATCGCGG TCCTGTACGT GGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACAGAG TTCCTCACGG GGCTGGGCAA GCTCATCGAG 
CTCAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG CGGCGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CTGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAC 
GACTTCGTGT CCATCGTCTA CAACGACTCT GGCGAGGACT TCAAGTTGGG CACCATCAAG 
GGCCAATTCA ACTTCGTCCA CGTGATTATC ACGCCCCTGG ACTACGAGTG CAACCTGGTG 
TCTCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGATA CCAGCATGGC CAAGATCGTG 
TCTGACCGAA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCCAA TATGGCCTCG 
CAGGTGCACC ATAGCCGCTC CAATCCCACC GACATTTATC CCTCCAAGTG GATTGCCAGG 
CTCCGCCACA TCAAGCGGCT GCGCCAGCGG ATCCGTGAGG AAGCCCACTA CTCAAGCGCC 
AGCCTGCCCC TGATGCAGAC ACACCCTCCT GGTCATGCCA AGGCTCCGGC ACAGGCCCCG 
GCCGAGTCCA CGCCCACCTA TGAGACGGGC CAGCGGAAAC GCCTGGTCTC CTCTGTGGAC 
GACTTCACTG AGTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315736.1
CDS	650..5590
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460250.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460250.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGGATCC AGACCTCTCT CACGAGCGCC AGCTTGGGGT CTGCAGACGA GAACTCGATG 
GCCCAGGCCG ACGACAACTT GAAAAACCTC CACCTGGAGC TCACAGAGAC GTGTCTGGAC 
ATGATGGCCA GATATGTGTT CTCCAACTTC ACGGCGGTCC CCAAGAGGTC CCCCGTGGGA 
GAGTTCCTCC TGGCTGGTGG CAGGACCAAA ACCTGGCTGG TTGGGAACAA GCTTGTCACC 
GTGACGACAA GCGTGGGGAC CGGGACCCGG TCACTGCTAG GCCTGGACTC TGGAGAGCTG 
CAGGGTGGCC CGGAGTTGAG CTCTGACCCC AGCATGCATG TGAGACAGAC AAAGGAGGCA 
CCCGCCAAGC TGGAGTCCCA AGCTGGGCAG CAGGTGTACC ACGGAGCCCG GGACCGGGTC 
CGCTCCATGT CCGGGGGCCA TGGCCTTCGC GTGGGTGCCT TGGACGCTCC AGCCTGCTAC 
TTCCCCAGCA GCCCCACGTC CCCGGGCTCC CAGACCGCTC CAGCCAGCCA GCCCGAGAAG 
GCCTCAGCTG GCAGCCCGCT CCCAGCACAG AAGGAGAAGA CAAACCTGGC CGCTTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCAGAGATC CTGGTCCGCA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCCT CGGACATCAA CAGCATGCCG 
CTGCAGGAGC TGTCCAATGC CCTCATGGCC GCCGAGCGCT TCAAGGAGCG CCGCGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCGGCG GCCGGCTCAG CCAAGCCATC CCCGCCCCCA 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTGCCAGT CCAGGTGCCA AGGAAAGCTG 
CACAGGAGCA TTTCCTGGGC AGATTCTGCG GTGGTCCTGG AGGAGGGAGG TCCAGGTGAG 
GCTGGTTTGT CTGCAGAGCC TCCCGAGTTG GAAGATTTTG AGGCGACACT GGGCTCCGAC 
GGGCGCTGTG GGCGCAGCGA CGCTTTCAGC AGGTCATCTT CCACCTCCAG CCAGGAGGAG 
AAGTCATTCC ATGCCGAGGA GTTGCCTCCT GGGGGCATCC CCATCGAGCG GGCGGTCTCG 
GAGGGCTCCC GGGCCTCCGT GGACCTCGCC TTCCAGCCCT CTCAGCCCCT GAGCAAGTCC 
AGCTCGTCGC CTGAACTGCA GACCCTGCAG GACATCCTGG GGGACCCCGG GGACAAGGCT 
GAGGTTGGCC GGCTGAGCCC CGAGGCCAAG GCCCGGTCGC AGTCAGGGAT CCTGGACGGG 
GAAGGTGCCG CCTGGTCAGC CCCGGGTGAG GAACGCCGGG GTCGGGGCCC TGCCCAGCCA 
GAGGGCCCCC TGCCTTCCAG CTGTCCTCGC TCCCCCAGCG GGCTGCGGCC CCGAGGCTAC 
ACCATCTCGG ATTCGGCCCC GTCACGCAGG GGCAAGAGGG TGGAGAGGGA TGCCTTCAAG 
AACCGAGCAG GGACCTCCAA CACCGAGAAG GTGCCAGGCA TCAACCCCAG CTTCGTGTTC 
CTGCAGCTCT ACCACTCACC GTTCTTTGGT GACGAGTCCA ACAAGCCAAT CCTTTTGCCT 
AACGAGTCTT TCGAACGATC AGTGCAGCTC CTTGATCAGA TCCCGTCGTA CGACACGCAC 
AAGATCGCGG TCCTGTACGT GGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACAGAG TTCCTCACGG GGCTGGGCAA GCTCATCGAG 
CTCAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG CGGCGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CTGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAC 
GACTTCGTGT CCATCGTCTA CAACGACTCT GGCGAGGACT TCAAGTTGGG CACCATCAAG 
GGCCAATTCA ACTTCGTCCA CGTGATTATC ACGCCCCTGG ACTACGAGTG CAACCTGGTG 
TCTCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGATA CCAGCATGGC CAAGATCGTG 
TCTGACCGAA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCCAA TATGGCCTCG 
CAGGTGCACC ATAGCCGCTC CAATCCCACC GACATTTATC CCTCCAAGTG GATTGCCAGG 
CTCCGCCACA TCAAGCGGCT GCGCCAGCGG ATCCGTGAGG AAGCCCACTA CTCAAGCGCC 
AGCCTGCCCC TGATGCAGAC ACACCCTCCT GGTCATGCCA AGGCTCCGGC ACAGGCCCCG 
GCCGAGTCCA CGCCCACCTA TGAGACGGGC CAGCGGAAAC GCCTGGTCTC CTCTGTGGAC 
GACTTCACTG AGTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015469255.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324741.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

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CloneID	OBa88434
Clone ID Related Accession (Same CDS sequence)	XM_015460250.1 , XM_015469255.1
Accession Version	XM_015469255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGGATCC AGACCTCTCT CACGAGCGCC AGCTTGGGGT CTGCAGACGA GAACTCGATG 
GCCCAGGCCG ACGACAACTT GAAAAACCTC CACCTGGAGC TCACAGAGAC GTGTCTGGAC 
ATGATGGCCA GATATGTGTT CTCCAACTTC ACGGCGGTCC CCAAGAGGTC CCCCGTGGGA 
GAGTTCCTCC TGGCTGGTGG CAGGACCAAA ACCTGGCTGG TTGGGAACAA GCTTGTCACC 
GTGACGACAA GCGTGGGGAC CGGGACCCGG TCACTGCTAG GCCTGGACTC TGGAGAGCTG 
CAGGGTGGCC CGGAGTTGAG CTCTGACCCC AGCATGCATG TGAGACAGAC AAAGGAGGCA 
CCCGCCAAGC TGGAGTCCCA AGCTGGGCAG CAGGTGTACC ACGGAGCCCG GGACCGGGTC 
CGCTCCATGT CCGGGGGCCA TGGCCTTCGC GTGGGTGCCT TGGACGCTCC AGCCTGCTAC 
TTCCCCAGCA GCCCCACGTC CCCGGGCTCC CAGACCGCTC CAGCCAGCCA GCCCGAGAAG 
GCCTCAGCTG GCAGCCCGCT CCCAGCACAG AAGGAGAAGA CAAACCTGGC CGCTTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCAGAGATC CTGGTCCGCA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCCT CGGACATCAA CAGCATGCCG 
CTGCAGGAGC TGTCCAATGC CCTCATGGCC GCCGAGCGCT TCAAGGAGCG CCGCGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCGGCG GCCGGCTCAG CCAAGCCATC CCCGCCCCCA 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTGCCAGT CCAGGTGCCA AGGAAAGCTG 
CACAGGAGCA TTTCCTGGGC AGATTCTGCG GTGGTCCTGG AGGAGGGAGG TCCAGGTGAG 
GCTGGTTTGT CTGCAGAGCC TCCCGAGTTG GAAGATTTTG AGGCGACACT GGGCTCCGAC 
GGGCGCTGTG GGCGCAGCGA CGCTTTCAGC AGGTCATCTT CCACCTCCAG CCAGGAGGAG 
AAGTCATTCC ATGCCGAGGA GTTGCCTCCT GGGGGCATCC CCATCGAGCG GGCGGTCTCG 
GAGGGCTCCC GGGCCTCCGT GGACCTCGCC TTCCAGCCCT CTCAGCCCCT GAGCAAGTCC 
AGCTCGTCGC CTGAACTGCA GACCCTGCAG GACATCCTGG GGGACCCCGG GGACAAGGCT 
GAGGTTGGCC GGCTGAGCCC CGAGGCCAAG GCCCGGTCGC AGTCAGGGAT CCTGGACGGG 
GAAGGTGCCG CCTGGTCAGC CCCGGGTGAG GAACGCCGGG GTCGGGGCCC TGCCCAGCCA 
GAGGGCCCCC TGCCTTCCAG CTGTCCTCGC TCCCCCAGCG GGCTGCGGCC CCGAGGCTAC 
ACCATCTCGG ATTCGGCCCC GTCACGCAGG GGCAAGAGGG TGGAGAGGGA TGCCTTCAAG 
AACCGAGCAG GGACCTCCAA CACCGAGAAG GTGCCAGGCA TCAACCCCAG CTTCGTGTTC 
CTGCAGCTCT ACCACTCACC GTTCTTTGGT GACGAGTCCA ACAAGCCAAT CCTTTTGCCT 
AACGAGTCTT TCGAACGATC AGTGCAGCTC CTTGATCAGA TCCCGTCGTA CGACACGCAC 
AAGATCGCGG TCCTGTACGT GGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACAGAG TTCCTCACGG GGCTGGGCAA GCTCATCGAG 
CTCAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG CGGCGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CTGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAC 
GACTTCGTGT CCATCGTCTA CAACGACTCT GGCGAGGACT TCAAGTTGGG CACCATCAAG 
GGCCAATTCA ACTTCGTCCA CGTGATTATC ACGCCCCTGG ACTACGAGTG CAACCTGGTG 
TCTCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGATA CCAGCATGGC CAAGATCGTG 
TCTGACCGAA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCCAA TATGGCCTCG 
CAGGTGCACC ATAGCCGCTC CAATCCCACC GACATTTATC CCTCCAAGTG GATTGCCAGG 
CTCCGCCACA TCAAGCGGCT GCGCCAGCGG ATCCGTGAGG AAGCCCACTA CTCAAGCGCC 
AGCCTGCCCC TGATGCAGAC ACACCCTCCT GGTCATGCCA AGGCTCCGGC ACAGGCCCCG 
GCCGAGTCCA CGCCCACCTA TGAGACGGGC CAGCGGAAAC GCCTGGTCTC CTCTGTGGAC 
GACTTCACTG AGTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324741.1
CDS	650..5590
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015469255.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469255.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGGATCC AGACCTCTCT CACGAGCGCC AGCTTGGGGT CTGCAGACGA GAACTCGATG 
GCCCAGGCCG ACGACAACTT GAAAAACCTC CACCTGGAGC TCACAGAGAC GTGTCTGGAC 
ATGATGGCCA GATATGTGTT CTCCAACTTC ACGGCGGTCC CCAAGAGGTC CCCCGTGGGA 
GAGTTCCTCC TGGCTGGTGG CAGGACCAAA ACCTGGCTGG TTGGGAACAA GCTTGTCACC 
GTGACGACAA GCGTGGGGAC CGGGACCCGG TCACTGCTAG GCCTGGACTC TGGAGAGCTG 
CAGGGTGGCC CGGAGTTGAG CTCTGACCCC AGCATGCATG TGAGACAGAC AAAGGAGGCA 
CCCGCCAAGC TGGAGTCCCA AGCTGGGCAG CAGGTGTACC ACGGAGCCCG GGACCGGGTC 
CGCTCCATGT CCGGGGGCCA TGGCCTTCGC GTGGGTGCCT TGGACGCTCC AGCCTGCTAC 
TTCCCCAGCA GCCCCACGTC CCCGGGCTCC CAGACCGCTC CAGCCAGCCA GCCCGAGAAG 
GCCTCAGCTG GCAGCCCGCT CCCAGCACAG AAGGAGAAGA CAAACCTGGC CGCTTACGTG 
CCCCTGCTGA CCCAGGGCTG GGCAGAGATC CTGGTCCGCA GGCCCACAGG GAACACCAGC 
TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCCT CGGACATCAA CAGCATGCCG 
CTGCAGGAGC TGTCCAATGC CCTCATGGCC GCCGAGCGCT TCAAGGAGCG CCGCGACACA 
GCCCTGTACA AGTCGCTGTC GGTGCCGGCG GCCGGCTCAG CCAAGCCATC CCCGCCCCCA 
CGCTCCAACA CAGTGGCCTC TTTCTCCTCC CTGTGCCAGT CCAGGTGCCA AGGAAAGCTG 
CACAGGAGCA TTTCCTGGGC AGATTCTGCG GTGGTCCTGG AGGAGGGAGG TCCAGGTGAG 
GCTGGTTTGT CTGCAGAGCC TCCCGAGTTG GAAGATTTTG AGGCGACACT GGGCTCCGAC 
GGGCGCTGTG GGCGCAGCGA CGCTTTCAGC AGGTCATCTT CCACCTCCAG CCAGGAGGAG 
AAGTCATTCC ATGCCGAGGA GTTGCCTCCT GGGGGCATCC CCATCGAGCG GGCGGTCTCG 
GAGGGCTCCC GGGCCTCCGT GGACCTCGCC TTCCAGCCCT CTCAGCCCCT GAGCAAGTCC 
AGCTCGTCGC CTGAACTGCA GACCCTGCAG GACATCCTGG GGGACCCCGG GGACAAGGCT 
GAGGTTGGCC GGCTGAGCCC CGAGGCCAAG GCCCGGTCGC AGTCAGGGAT CCTGGACGGG 
GAAGGTGCCG CCTGGTCAGC CCCGGGTGAG GAACGCCGGG GTCGGGGCCC TGCCCAGCCA 
GAGGGCCCCC TGCCTTCCAG CTGTCCTCGC TCCCCCAGCG GGCTGCGGCC CCGAGGCTAC 
ACCATCTCGG ATTCGGCCCC GTCACGCAGG GGCAAGAGGG TGGAGAGGGA TGCCTTCAAG 
AACCGAGCAG GGACCTCCAA CACCGAGAAG GTGCCAGGCA TCAACCCCAG CTTCGTGTTC 
CTGCAGCTCT ACCACTCACC GTTCTTTGGT GACGAGTCCA ACAAGCCAAT CCTTTTGCCT 
AACGAGTCTT TCGAACGATC AGTGCAGCTC CTTGATCAGA TCCCGTCGTA CGACACGCAC 
AAGATCGCGG TCCTGTACGT GGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACAGAG TTCCTCACGG GGCTGGGCAA GCTCATCGAG 
CTCAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGCGGCC TGGACGTGTG CGGCGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGACGAC ATCATGCAAG CTGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAC 
GACTTCGTGT CCATCGTCTA CAACGACTCT GGCGAGGACT TCAAGTTGGG CACCATCAAG 
GGCCAATTCA ACTTCGTCCA CGTGATTATC ACGCCCCTGG ACTACGAGTG CAACCTGGTG 
TCTCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGATA CCAGCATGGC CAAGATCGTG 
TCTGACCGAA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCCAA TATGGCCTCG 
CAGGTGCACC ATAGCCGCTC CAATCCCACC GACATTTATC CCTCCAAGTG GATTGCCAGG 
CTCCGCCACA TCAAGCGGCT GCGCCAGCGG ATCCGTGAGG AAGCCCACTA CTCAAGCGCC 
AGCCTGCCCC TGATGCAGAC ACACCCTCCT GGTCATGCCA AGGCTCCGGC ACAGGCCCCG 
GCCGAGTCCA CGCCCACCTA TGAGACGGGC CAGCGGAAAC GCCTGGTCTC CTCTGTGGAC 
GACTTCACTG AGTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003584006.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003584054.2 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

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CloneID	OBa88435
Clone ID Related Accession (Same CDS sequence)	XM_003584006.4 , XM_015460251.1
Accession Version	XM_003584006.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003584006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003584054.2
CDS	650..5524
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003584006.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003584006.4

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460251.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315737.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

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CloneID	OBa88435
Clone ID Related Accession (Same CDS sequence)	XM_003584006.4 , XM_015460251.1
Accession Version	XM_015460251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315737.1
CDS	650..5524
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460251.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460251.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG 
AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAACTCAAG 
GAGAGCTCTG CAGCCGATGC CTTCCGGTGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC 
CGCAGCAGGA TCCAGACCTC TCTCACGAGC GCCAGCTTGG GGTCTGCAGA CGAGAACTCG 
ATGGCCCAGG CCGACGACAA CTTGAAAAAC CTCCACCTGG AGCTCACAGA GACGTGTCTG 
GACATGATGG CCAGATATGT GTTCTCCAAC TTCACGGCGG TCCCCAAGAG GTCCCCCGTG 
GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC 
ACCGTGACGA CAAGCGTGGG GACCGGGACC CGGTCACTGC TAGGCCTGGA CTCTGGAGAG 
CTGCAGGGTG GCCCGGAGTT GAGCTCTGAC CCCAGCATGC ATGTGAGACA GACAAAGGAG 
GCACCCGCCA AGCTGGAGTC CCAAGCTGGG CAGCAGGTGT ACCACGGAGC CCGGGACCGG 
GTCCGCTCCA TGTCCGGGGG CCATGGCCTT CGCGTGGGTG CCTTGGACGC TCCAGCCTGC 
TACTTCCCCA GCAGCCCCAC GTCCCCGGGC TCCCAGACCG CTCCAGCCAG CCAGCCCGAG 
AAGGCCTCAG CTGGCAGCCC GCTCCCAGCA CAGAAGGAGA AGACAAACCT GGCCGCTTAC 
GTGCCCCTGC TGACCCAGGG CTGGGCAGAG ATCCTGGTCC GCAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCCTTCT CCTCGGACAT CAACAGCATG 
CCGCTGCAGG AGCTGTCCAA TGCCCTCATG GCCGCCGAGC GCTTCAAGGA GCGCCGCGAC 
ACAGCCCTGT ACAAGTCGCT GTCGGTGCCG GCGGCCGGCT CAGCCAAGCC ATCCCCGCCC 
CCACGCTCCA ACACAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015469256.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324742.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OBa88436
Clone ID Related Accession (Same CDS sequence)	XM_015469256.1 , XM_015460252.1
Accession Version	XM_015469256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGTGG CCTCTTTCTC CTCCCTGTGC CAGTCCAGGT GCCAAGGAAA GCTGCACAGG 
AGCATTTCCT GGGCAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324742.1
CDS	650..5464
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015469256.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469256.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGTGG CCTCTTTCTC CTCCCTGTGC CAGTCCAGGT GCCAAGGAAA GCTGCACAGG 
AGCATTTCCT GGGCAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460252.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315738.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa88436
Clone ID Related Accession (Same CDS sequence)	XM_015469256.1 , XM_015460252.1
Accession Version	XM_015460252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGTGG CCTCTTTCTC CTCCCTGTGC CAGTCCAGGT GCCAAGGAAA GCTGCACAGG 
AGCATTTCCT GGGCAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315738.1
CDS	650..5464
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460252.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460252.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGTGG CCTCTTTCTC CTCCCTGTGC CAGTCCAGGT GCCAAGGAAA GCTGCACAGG 
AGCATTTCCT GGGCAGATTC TGCGGTGGTC CTGGAGGAGG GAGGTCCAGG TGAGGCTGGT 
TTGTCTGCAG AGCCTCCCGA GTTGGAAGAT TTTGAGGCGA CACTGGGCTC CGACGGGCGC 
TGTGGGCGCA GCGACGCTTT CAGCAGGTCA TCTTCCACCT CCAGCCAGGA GGAGAAGTCA 
TTCCATGCCG AGGAGTTGCC TCCTGGGGGC ATCCCCATCG AGCGGGCGGT CTCGGAGGGC 
TCCCGGGCCT CCGTGGACCT CGCCTTCCAG CCCTCTCAGC CCCTGAGCAA GTCCAGCTCG 
TCGCCTGAAC TGCAGACCCT GCAGGACATC CTGGGGGACC CCGGGGACAA GGCTGAGGTT 
GGCCGGCTGA GCCCCGAGGC CAAGGCCCGG TCGCAGTCAG GGATCCTGGA CGGGGAAGGT 
GCCGCCTGGT CAGCCCCGGG TGAGGAACGC CGGGGTCGGG GCCCTGCCCA GCCAGAGGGC 
CCCCTGCCTT CCAGCTGTCC TCGCTCCCCC AGCGGGCTGC GGCCCCGAGG CTACACCATC 
TCGGATTCGG CCCCGTCACG CAGGGGCAAG AGGGTGGAGA GGGATGCCTT CAAGAACCGA 
GCAGGGACCT CCAACACCGA GAAGGTGCCA GGCATCAACC CCAGCTTCGT GTTCCTGCAG 
CTCTACCACT CACCGTTCTT TGGTGACGAG TCCAACAAGC CAATCCTTTT GCCTAACGAG 
TCTTTCGAAC GATCAGTGCA GCTCCTTGAT CAGATCCCGT CGTACGACAC GCACAAGATC 
GCGGTCCTGT ACGTGGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAACGAG 
CATGGCTCCT ACAGGTACAC AGAGTTCCTC ACGGGGCTGG GCAAGCTCAT CGAGCTCAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGC GGCCTGGACG TGTGCGGCGA GGATGGCCAG 
TTCACCTACT GCTGGCACGA CGACATCATG CAAGCTGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GCCACCTGGG CAACGACTTC 
GTGTCCATCG TCTACAACGA CTCTGGCGAG GACTTCAAGT TGGGCACCAT CAAGGGCCAA 
TTCAACTTCG TCCACGTGAT TATCACGCCC CTGGACTACG AGTGCAACCT GGTGTCTCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GATACCAGCA TGGCCAAGAT CGTGTCTGAC 
CGAAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CCAATATGGC CTCGCAGGTG 
CACCATAGCC GCTCCAATCC CACCGACATT TATCCCTCCA AGTGGATTGC CAGGCTCCGC 
CACATCAAGC GGCTGCGCCA GCGGATCCGT GAGGAAGCCC ACTACTCAAG CGCCAGCCTG 
CCCCTGATGC AGACACACCC TCCTGGTCAT GCCAAGGCTC CGGCACAGGC CCCGGCCGAG 
TCCACGCCCA CCTATGAGAC GGGCCAGCGG AAACGCCTGG TCTCCTCTGT GGACGACTTC 
ACTGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460253.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315739.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa88437
Clone ID Related Accession (Same CDS sequence)	XM_015460253.1 , XM_015469257.1
Accession Version	XM_015460253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG TCCAGGTGCC AAGGAAAGCT GCACAGGAGC 
ATTTCCTGGG CAGATTCTGC GGTGGTCCTG GAGGAGGGAG GTCCAGGTGA GGCTGGTTTG 
TCTGCAGAGC CTCCCGAGTT GGAAGATTTT GAGGCGACAC TGGGCTCCGA CGGGCGCTGT 
GGGCGCAGCG ACGCTTTCAG CAGGTCATCT TCCACCTCCA GCCAGGAGGA GAAGTCATTC 
CATGCCGAGG AGTTGCCTCC TGGGGGCATC CCCATCGAGC GGGCGGTCTC GGAGGGCTCC 
CGGGCCTCCG TGGACCTCGC CTTCCAGCCC TCTCAGCCCC TGAGCAAGTC CAGCTCGTCG 
CCTGAACTGC AGACCCTGCA GGACATCCTG GGGGACCCCG GGGACAAGGC TGAGGTTGGC 
CGGCTGAGCC CCGAGGCCAA GGCCCGGTCG CAGTCAGGGA TCCTGGACGG GGAAGGTGCC 
GCCTGGTCAG CCCCGGGTGA GGAACGCCGG GGTCGGGGCC CTGCCCAGCC AGAGGGCCCC 
CTGCCTTCCA GCTGTCCTCG CTCCCCCAGC GGGCTGCGGC CCCGAGGCTA CACCATCTCG 
GATTCGGCCC CGTCACGCAG GGGCAAGAGG GTGGAGAGGG ATGCCTTCAA GAACCGAGCA 
GGGACCTCCA ACACCGAGAA GGTGCCAGGC ATCAACCCCA GCTTCGTGTT CCTGCAGCTC 
TACCACTCAC CGTTCTTTGG TGACGAGTCC AACAAGCCAA TCCTTTTGCC TAACGAGTCT 
TTCGAACGAT CAGTGCAGCT CCTTGATCAG ATCCCGTCGT ACGACACGCA CAAGATCGCG 
GTCCTGTACG TGGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT 
GGCTCCTACA GGTACACAGA GTTCCTCACG GGGCTGGGCA AGCTCATCGA GCTCAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GCGGCGAGGA TGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCTGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGTGAC AAGAAGCGCC ACCTGGGCAA CGACTTCGTG 
TCCATCGTCT ACAACGACTC TGGCGAGGAC TTCAAGTTGG GCACCATCAA GGGCCAATTC 
AACTTCGTCC ACGTGATTAT CACGCCCCTG GACTACGAGT GCAACCTGGT GTCTCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAT ACCAGCATGG CCAAGATCGT GTCTGACCGA 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCCA ATATGGCCTC GCAGGTGCAC 
CATAGCCGCT CCAATCCCAC CGACATTTAT CCCTCCAAGT GGATTGCCAG GCTCCGCCAC 
ATCAAGCGGC TGCGCCAGCG GATCCGTGAG GAAGCCCACT ACTCAAGCGC CAGCCTGCCC 
CTGATGCAGA CACACCCTCC TGGTCATGCC AAGGCTCCGG CACAGGCCCC GGCCGAGTCC 
ACGCCCACCT ATGAGACGGG CCAGCGGAAA CGCCTGGTCT CCTCTGTGGA CGACTTCACT 
GAGTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315739.1
CDS	650..5461
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460253.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460253.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG TCCAGGTGCC AAGGAAAGCT GCACAGGAGC 
ATTTCCTGGG CAGATTCTGC GGTGGTCCTG GAGGAGGGAG GTCCAGGTGA GGCTGGTTTG 
TCTGCAGAGC CTCCCGAGTT GGAAGATTTT GAGGCGACAC TGGGCTCCGA CGGGCGCTGT 
GGGCGCAGCG ACGCTTTCAG CAGGTCATCT TCCACCTCCA GCCAGGAGGA GAAGTCATTC 
CATGCCGAGG AGTTGCCTCC TGGGGGCATC CCCATCGAGC GGGCGGTCTC GGAGGGCTCC 
CGGGCCTCCG TGGACCTCGC CTTCCAGCCC TCTCAGCCCC TGAGCAAGTC CAGCTCGTCG 
CCTGAACTGC AGACCCTGCA GGACATCCTG GGGGACCCCG GGGACAAGGC TGAGGTTGGC 
CGGCTGAGCC CCGAGGCCAA GGCCCGGTCG CAGTCAGGGA TCCTGGACGG GGAAGGTGCC 
GCCTGGTCAG CCCCGGGTGA GGAACGCCGG GGTCGGGGCC CTGCCCAGCC AGAGGGCCCC 
CTGCCTTCCA GCTGTCCTCG CTCCCCCAGC GGGCTGCGGC CCCGAGGCTA CACCATCTCG 
GATTCGGCCC CGTCACGCAG GGGCAAGAGG GTGGAGAGGG ATGCCTTCAA GAACCGAGCA 
GGGACCTCCA ACACCGAGAA GGTGCCAGGC ATCAACCCCA GCTTCGTGTT CCTGCAGCTC 
TACCACTCAC CGTTCTTTGG TGACGAGTCC AACAAGCCAA TCCTTTTGCC TAACGAGTCT 
TTCGAACGAT CAGTGCAGCT CCTTGATCAG ATCCCGTCGT ACGACACGCA CAAGATCGCG 
GTCCTGTACG TGGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT 
GGCTCCTACA GGTACACAGA GTTCCTCACG GGGCTGGGCA AGCTCATCGA GCTCAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GCGGCGAGGA TGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCTGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGTGAC AAGAAGCGCC ACCTGGGCAA CGACTTCGTG 
TCCATCGTCT ACAACGACTC TGGCGAGGAC TTCAAGTTGG GCACCATCAA GGGCCAATTC 
AACTTCGTCC ACGTGATTAT CACGCCCCTG GACTACGAGT GCAACCTGGT GTCTCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAT ACCAGCATGG CCAAGATCGT GTCTGACCGA 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCCA ATATGGCCTC GCAGGTGCAC 
CATAGCCGCT CCAATCCCAC CGACATTTAT CCCTCCAAGT GGATTGCCAG GCTCCGCCAC 
ATCAAGCGGC TGCGCCAGCG GATCCGTGAG GAAGCCCACT ACTCAAGCGC CAGCCTGCCC 
CTGATGCAGA CACACCCTCC TGGTCATGCC AAGGCTCCGG CACAGGCCCC GGCCGAGTCC 
ACGCCCACCT ATGAGACGGG CCAGCGGAAA CGCCTGGTCT CCTCTGTGGA CGACTTCACT 
GAGTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015469257.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324743.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OBa88437
Clone ID Related Accession (Same CDS sequence)	XM_015460253.1 , XM_015469257.1
Accession Version	XM_015469257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG TCCAGGTGCC AAGGAAAGCT GCACAGGAGC 
ATTTCCTGGG CAGATTCTGC GGTGGTCCTG GAGGAGGGAG GTCCAGGTGA GGCTGGTTTG 
TCTGCAGAGC CTCCCGAGTT GGAAGATTTT GAGGCGACAC TGGGCTCCGA CGGGCGCTGT 
GGGCGCAGCG ACGCTTTCAG CAGGTCATCT TCCACCTCCA GCCAGGAGGA GAAGTCATTC 
CATGCCGAGG AGTTGCCTCC TGGGGGCATC CCCATCGAGC GGGCGGTCTC GGAGGGCTCC 
CGGGCCTCCG TGGACCTCGC CTTCCAGCCC TCTCAGCCCC TGAGCAAGTC CAGCTCGTCG 
CCTGAACTGC AGACCCTGCA GGACATCCTG GGGGACCCCG GGGACAAGGC TGAGGTTGGC 
CGGCTGAGCC CCGAGGCCAA GGCCCGGTCG CAGTCAGGGA TCCTGGACGG GGAAGGTGCC 
GCCTGGTCAG CCCCGGGTGA GGAACGCCGG GGTCGGGGCC CTGCCCAGCC AGAGGGCCCC 
CTGCCTTCCA GCTGTCCTCG CTCCCCCAGC GGGCTGCGGC CCCGAGGCTA CACCATCTCG 
GATTCGGCCC CGTCACGCAG GGGCAAGAGG GTGGAGAGGG ATGCCTTCAA GAACCGAGCA 
GGGACCTCCA ACACCGAGAA GGTGCCAGGC ATCAACCCCA GCTTCGTGTT CCTGCAGCTC 
TACCACTCAC CGTTCTTTGG TGACGAGTCC AACAAGCCAA TCCTTTTGCC TAACGAGTCT 
TTCGAACGAT CAGTGCAGCT CCTTGATCAG ATCCCGTCGT ACGACACGCA CAAGATCGCG 
GTCCTGTACG TGGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT 
GGCTCCTACA GGTACACAGA GTTCCTCACG GGGCTGGGCA AGCTCATCGA GCTCAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GCGGCGAGGA TGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCTGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGTGAC AAGAAGCGCC ACCTGGGCAA CGACTTCGTG 
TCCATCGTCT ACAACGACTC TGGCGAGGAC TTCAAGTTGG GCACCATCAA GGGCCAATTC 
AACTTCGTCC ACGTGATTAT CACGCCCCTG GACTACGAGT GCAACCTGGT GTCTCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAT ACCAGCATGG CCAAGATCGT GTCTGACCGA 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCCA ATATGGCCTC GCAGGTGCAC 
CATAGCCGCT CCAATCCCAC CGACATTTAT CCCTCCAAGT GGATTGCCAG GCTCCGCCAC 
ATCAAGCGGC TGCGCCAGCG GATCCGTGAG GAAGCCCACT ACTCAAGCGC CAGCCTGCCC 
CTGATGCAGA CACACCCTCC TGGTCATGCC AAGGCTCCGG CACAGGCCCC GGCCGAGTCC 
ACGCCCACCT ATGAGACGGG CCAGCGGAAA CGCCTGGTCT CCTCTGTGGA CGACTTCACT 
GAGTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324743.1
CDS	650..5461
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTVASFSSLCQSRCQGKLHRSISWADSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015469257.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469257.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGTGGCCT CTTTCTCCTC CCTGTGCCAG TCCAGGTGCC AAGGAAAGCT GCACAGGAGC 
ATTTCCTGGG CAGATTCTGC GGTGGTCCTG GAGGAGGGAG GTCCAGGTGA GGCTGGTTTG 
TCTGCAGAGC CTCCCGAGTT GGAAGATTTT GAGGCGACAC TGGGCTCCGA CGGGCGCTGT 
GGGCGCAGCG ACGCTTTCAG CAGGTCATCT TCCACCTCCA GCCAGGAGGA GAAGTCATTC 
CATGCCGAGG AGTTGCCTCC TGGGGGCATC CCCATCGAGC GGGCGGTCTC GGAGGGCTCC 
CGGGCCTCCG TGGACCTCGC CTTCCAGCCC TCTCAGCCCC TGAGCAAGTC CAGCTCGTCG 
CCTGAACTGC AGACCCTGCA GGACATCCTG GGGGACCCCG GGGACAAGGC TGAGGTTGGC 
CGGCTGAGCC CCGAGGCCAA GGCCCGGTCG CAGTCAGGGA TCCTGGACGG GGAAGGTGCC 
GCCTGGTCAG CCCCGGGTGA GGAACGCCGG GGTCGGGGCC CTGCCCAGCC AGAGGGCCCC 
CTGCCTTCCA GCTGTCCTCG CTCCCCCAGC GGGCTGCGGC CCCGAGGCTA CACCATCTCG 
GATTCGGCCC CGTCACGCAG GGGCAAGAGG GTGGAGAGGG ATGCCTTCAA GAACCGAGCA 
GGGACCTCCA ACACCGAGAA GGTGCCAGGC ATCAACCCCA GCTTCGTGTT CCTGCAGCTC 
TACCACTCAC CGTTCTTTGG TGACGAGTCC AACAAGCCAA TCCTTTTGCC TAACGAGTCT 
TTCGAACGAT CAGTGCAGCT CCTTGATCAG ATCCCGTCGT ACGACACGCA CAAGATCGCG 
GTCCTGTACG TGGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT 
GGCTCCTACA GGTACACAGA GTTCCTCACG GGGCTGGGCA AGCTCATCGA GCTCAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGCGGC CTGGACGTGT GCGGCGAGGA TGGCCAGTTC 
ACCTACTGCT GGCACGACGA CATCATGCAA GCTGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGTGAC AAGAAGCGCC ACCTGGGCAA CGACTTCGTG 
TCCATCGTCT ACAACGACTC TGGCGAGGAC TTCAAGTTGG GCACCATCAA GGGCCAATTC 
AACTTCGTCC ACGTGATTAT CACGCCCCTG GACTACGAGT GCAACCTGGT GTCTCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAT ACCAGCATGG CCAAGATCGT GTCTGACCGA 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCCA ATATGGCCTC GCAGGTGCAC 
CATAGCCGCT CCAATCCCAC CGACATTTAT CCCTCCAAGT GGATTGCCAG GCTCCGCCAC 
ATCAAGCGGC TGCGCCAGCG GATCCGTGAG GAAGCCCACT ACTCAAGCGC CAGCCTGCCC 
CTGATGCAGA CACACCCTCC TGGTCATGCC AAGGCTCCGG CACAGGCCCC GGCCGAGTCC 
ACGCCCACCT ATGAGACGGG CCAGCGGAAA CGCCTGGTCT CCTCTGTGGA CGACTTCACT 
GAGTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005196829.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196886.2 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

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CloneID	OBa88438
Clone ID Related Accession (Same CDS sequence)	XM_005196829.3 , XM_015460255.1
Accession Version	XM_005196829.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGATT CTGCGGTGGT CCTGGAGGAG GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA 
GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG ACACTGGGCT CCGACGGGCG CTGTGGGCGC 
AGCGACGCTT TCAGCAGGTC ATCTTCCACC TCCAGCCAGG AGGAGAAGTC ATTCCATGCC 
GAGGAGTTGC CTCCTGGGGG CATCCCCATC GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC 
TCCGTGGACC TCGCCTTCCA GCCCTCTCAG CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA 
CTGCAGACCC TGCAGGACAT CCTGGGGGAC CCCGGGGACA AGGCTGAGGT TGGCCGGCTG 
AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA GGGATCCTGG ACGGGGAAGG TGCCGCCTGG 
TCAGCCCCGG GTGAGGAACG CCGGGGTCGG GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT 
TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG CGGCCCCGAG GCTACACCAT CTCGGATTCG 
GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG AGGGATGCCT TCAAGAACCG AGCAGGGACC 
TCCAACACCG AGAAGGTGCC AGGCATCAAC CCCAGCTTCG TGTTCCTGCA GCTCTACCAC 
TCACCGTTCT TTGGTGACGA GTCCAACAAG CCAATCCTTT TGCCTAACGA GTCTTTCGAA 
CGATCAGTGC AGCTCCTTGA TCAGATCCCG TCGTACGACA CGCACAAGAT CGCGGTCCTG 
TACGTGGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAACGA GCATGGCTCC 
TACAGGTACA CAGAGTTCCT CACGGGGCTG GGCAAGCTCA TCGAGCTCAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG CGGCCTGGAC GTGTGCGGCG AGGATGGCCA GTTCACCTAC 
TGCTGGCACG ACGACATCAT GCAAGCTGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG TGACAAGAAG CGCCACCTGG GCAACGACTT CGTGTCCATC 
GTCTACAACG ACTCTGGCGA GGACTTCAAG TTGGGCACCA TCAAGGGCCA ATTCAACTTC 
GTCCACGTGA TTATCACGCC CCTGGACTAC GAGTGCAACC TGGTGTCTCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGATACCAGC ATGGCCAAGA TCGTGTCTGA CCGAAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCCAATATGG CCTCGCAGGT GCACCATAGC 
CGCTCCAATC CCACCGACAT TTATCCCTCC AAGTGGATTG CCAGGCTCCG CCACATCAAG 
CGGCTGCGCC AGCGGATCCG TGAGGAAGCC CACTACTCAA GCGCCAGCCT GCCCCTGATG 
CAGACACACC CTCCTGGTCA TGCCAAGGCT CCGGCACAGG CCCCGGCCGA GTCCACGCCC 
ACCTATGAGA CGGGCCAGCG GAAACGCCTG GTCTCCTCTG TGGACGACTT CACTGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196886.2
CDS	650..5395
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005196829.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196829.3

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGATT CTGCGGTGGT CCTGGAGGAG GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA 
GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG ACACTGGGCT CCGACGGGCG CTGTGGGCGC 
AGCGACGCTT TCAGCAGGTC ATCTTCCACC TCCAGCCAGG AGGAGAAGTC ATTCCATGCC 
GAGGAGTTGC CTCCTGGGGG CATCCCCATC GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC 
TCCGTGGACC TCGCCTTCCA GCCCTCTCAG CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA 
CTGCAGACCC TGCAGGACAT CCTGGGGGAC CCCGGGGACA AGGCTGAGGT TGGCCGGCTG 
AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA GGGATCCTGG ACGGGGAAGG TGCCGCCTGG 
TCAGCCCCGG GTGAGGAACG CCGGGGTCGG GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT 
TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG CGGCCCCGAG GCTACACCAT CTCGGATTCG 
GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG AGGGATGCCT TCAAGAACCG AGCAGGGACC 
TCCAACACCG AGAAGGTGCC AGGCATCAAC CCCAGCTTCG TGTTCCTGCA GCTCTACCAC 
TCACCGTTCT TTGGTGACGA GTCCAACAAG CCAATCCTTT TGCCTAACGA GTCTTTCGAA 
CGATCAGTGC AGCTCCTTGA TCAGATCCCG TCGTACGACA CGCACAAGAT CGCGGTCCTG 
TACGTGGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAACGA GCATGGCTCC 
TACAGGTACA CAGAGTTCCT CACGGGGCTG GGCAAGCTCA TCGAGCTCAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG CGGCCTGGAC GTGTGCGGCG AGGATGGCCA GTTCACCTAC 
TGCTGGCACG ACGACATCAT GCAAGCTGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG TGACAAGAAG CGCCACCTGG GCAACGACTT CGTGTCCATC 
GTCTACAACG ACTCTGGCGA GGACTTCAAG TTGGGCACCA TCAAGGGCCA ATTCAACTTC 
GTCCACGTGA TTATCACGCC CCTGGACTAC GAGTGCAACC TGGTGTCTCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGATACCAGC ATGGCCAAGA TCGTGTCTGA CCGAAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCCAATATGG CCTCGCAGGT GCACCATAGC 
CGCTCCAATC CCACCGACAT TTATCCCTCC AAGTGGATTG CCAGGCTCCG CCACATCAAG 
CGGCTGCGCC AGCGGATCCG TGAGGAAGCC CACTACTCAA GCGCCAGCCT GCCCCTGATG 
CAGACACACC CTCCTGGTCA TGCCAAGGCT CCGGCACAGG CCCCGGCCGA GTCCACGCCC 
ACCTATGAGA CGGGCCAGCG GAAACGCCTG GTCTCCTCTG TGGACGACTT CACTGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460255.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315741.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

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CloneID	OBa88438
Clone ID Related Accession (Same CDS sequence)	XM_005196829.3 , XM_015460255.1
Accession Version	XM_015460255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGATT CTGCGGTGGT CCTGGAGGAG GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA 
GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG ACACTGGGCT CCGACGGGCG CTGTGGGCGC 
AGCGACGCTT TCAGCAGGTC ATCTTCCACC TCCAGCCAGG AGGAGAAGTC ATTCCATGCC 
GAGGAGTTGC CTCCTGGGGG CATCCCCATC GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC 
TCCGTGGACC TCGCCTTCCA GCCCTCTCAG CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA 
CTGCAGACCC TGCAGGACAT CCTGGGGGAC CCCGGGGACA AGGCTGAGGT TGGCCGGCTG 
AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA GGGATCCTGG ACGGGGAAGG TGCCGCCTGG 
TCAGCCCCGG GTGAGGAACG CCGGGGTCGG GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT 
TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG CGGCCCCGAG GCTACACCAT CTCGGATTCG 
GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG AGGGATGCCT TCAAGAACCG AGCAGGGACC 
TCCAACACCG AGAAGGTGCC AGGCATCAAC CCCAGCTTCG TGTTCCTGCA GCTCTACCAC 
TCACCGTTCT TTGGTGACGA GTCCAACAAG CCAATCCTTT TGCCTAACGA GTCTTTCGAA 
CGATCAGTGC AGCTCCTTGA TCAGATCCCG TCGTACGACA CGCACAAGAT CGCGGTCCTG 
TACGTGGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAACGA GCATGGCTCC 
TACAGGTACA CAGAGTTCCT CACGGGGCTG GGCAAGCTCA TCGAGCTCAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG CGGCCTGGAC GTGTGCGGCG AGGATGGCCA GTTCACCTAC 
TGCTGGCACG ACGACATCAT GCAAGCTGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG TGACAAGAAG CGCCACCTGG GCAACGACTT CGTGTCCATC 
GTCTACAACG ACTCTGGCGA GGACTTCAAG TTGGGCACCA TCAAGGGCCA ATTCAACTTC 
GTCCACGTGA TTATCACGCC CCTGGACTAC GAGTGCAACC TGGTGTCTCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGATACCAGC ATGGCCAAGA TCGTGTCTGA CCGAAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCCAATATGG CCTCGCAGGT GCACCATAGC 
CGCTCCAATC CCACCGACAT TTATCCCTCC AAGTGGATTG CCAGGCTCCG CCACATCAAG 
CGGCTGCGCC AGCGGATCCG TGAGGAAGCC CACTACTCAA GCGCCAGCCT GCCCCTGATG 
CAGACACACC CTCCTGGTCA TGCCAAGGCT CCGGCACAGG CCCCGGCCGA GTCCACGCCC 
ACCTATGAGA CGGGCCAGCG GAAACGCCTG GTCTCCTCTG TGGACGACTT CACTGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315741.1
CDS	650..5395
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460255.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460255.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGCAGG 
ATCCAGACCT CTCTCACGAG CGCCAGCTTG GGGTCTGCAG ACGAGAACTC GATGGCCCAG 
GCCGACGACA ACTTGAAAAA CCTCCACCTG GAGCTCACAG AGACGTGTCT GGACATGATG 
GCCAGATATG TGTTCTCCAA CTTCACGGCG GTCCCCAAGA GGTCCCCCGT GGGAGAGTTC 
CTCCTGGCTG GTGGCAGGAC CAAAACCTGG CTGGTTGGGA ACAAGCTTGT CACCGTGACG 
ACAAGCGTGG GGACCGGGAC CCGGTCACTG CTAGGCCTGG ACTCTGGAGA GCTGCAGGGT 
GGCCCGGAGT TGAGCTCTGA CCCCAGCATG CATGTGAGAC AGACAAAGGA GGCACCCGCC 
AAGCTGGAGT CCCAAGCTGG GCAGCAGGTG TACCACGGAG CCCGGGACCG GGTCCGCTCC 
ATGTCCGGGG GCCATGGCCT TCGCGTGGGT GCCTTGGACG CTCCAGCCTG CTACTTCCCC 
AGCAGCCCCA CGTCCCCGGG CTCCCAGACC GCTCCAGCCA GCCAGCCCGA GAAGGCCTCA 
GCTGGCAGCC CGCTCCCAGC ACAGAAGGAG AAGACAAACC TGGCCGCTTA CGTGCCCCTG 
CTGACCCAGG GCTGGGCAGA GATCCTGGTC CGCAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCCTTC TCCTCGGACA TCAACAGCAT GCCGCTGCAG 
GAGCTGTCCA ATGCCCTCAT GGCCGCCGAG CGCTTCAAGG AGCGCCGCGA CACAGCCCTG 
TACAAGTCGC TGTCGGTGCC GGCGGCCGGC TCAGCCAAGC CATCCCCGCC CCCACGCTCC 
AACACAGATT CTGCGGTGGT CCTGGAGGAG GGAGGTCCAG GTGAGGCTGG TTTGTCTGCA 
GAGCCTCCCG AGTTGGAAGA TTTTGAGGCG ACACTGGGCT CCGACGGGCG CTGTGGGCGC 
AGCGACGCTT TCAGCAGGTC ATCTTCCACC TCCAGCCAGG AGGAGAAGTC ATTCCATGCC 
GAGGAGTTGC CTCCTGGGGG CATCCCCATC GAGCGGGCGG TCTCGGAGGG CTCCCGGGCC 
TCCGTGGACC TCGCCTTCCA GCCCTCTCAG CCCCTGAGCA AGTCCAGCTC GTCGCCTGAA 
CTGCAGACCC TGCAGGACAT CCTGGGGGAC CCCGGGGACA AGGCTGAGGT TGGCCGGCTG 
AGCCCCGAGG CCAAGGCCCG GTCGCAGTCA GGGATCCTGG ACGGGGAAGG TGCCGCCTGG 
TCAGCCCCGG GTGAGGAACG CCGGGGTCGG GGCCCTGCCC AGCCAGAGGG CCCCCTGCCT 
TCCAGCTGTC CTCGCTCCCC CAGCGGGCTG CGGCCCCGAG GCTACACCAT CTCGGATTCG 
GCCCCGTCAC GCAGGGGCAA GAGGGTGGAG AGGGATGCCT TCAAGAACCG AGCAGGGACC 
TCCAACACCG AGAAGGTGCC AGGCATCAAC CCCAGCTTCG TGTTCCTGCA GCTCTACCAC 
TCACCGTTCT TTGGTGACGA GTCCAACAAG CCAATCCTTT TGCCTAACGA GTCTTTCGAA 
CGATCAGTGC AGCTCCTTGA TCAGATCCCG TCGTACGACA CGCACAAGAT CGCGGTCCTG 
TACGTGGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAACGA GCATGGCTCC 
TACAGGTACA CAGAGTTCCT CACGGGGCTG GGCAAGCTCA TCGAGCTCAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG CGGCCTGGAC GTGTGCGGCG AGGATGGCCA GTTCACCTAC 
TGCTGGCACG ACGACATCAT GCAAGCTGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG TGACAAGAAG CGCCACCTGG GCAACGACTT CGTGTCCATC 
GTCTACAACG ACTCTGGCGA GGACTTCAAG TTGGGCACCA TCAAGGGCCA ATTCAACTTC 
GTCCACGTGA TTATCACGCC CCTGGACTAC GAGTGCAACC TGGTGTCTCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGATACCAGC ATGGCCAAGA TCGTGTCTGA CCGAAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCCAATATGG CCTCGCAGGT GCACCATAGC 
CGCTCCAATC CCACCGACAT TTATCCCTCC AAGTGGATTG CCAGGCTCCG CCACATCAAG 
CGGCTGCGCC AGCGGATCCG TGAGGAAGCC CACTACTCAA GCGCCAGCCT GCCCCTGATG 
CAGACACACC CTCCTGGTCA TGCCAAGGCT CCGGCACAGG CCCCGGCCGA GTCCACGCCC 
ACCTATGAGA CGGGCCAGCG GAAACGCCTG GTCTCCTCTG TGGACGACTT CACTGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_003584005.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003584053.2 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa88439
Clone ID Related Accession (Same CDS sequence)	XM_003584005.4 , XM_015460256.1
Accession Version	XM_003584005.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003584005.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGATTCTG CGGTGGTCCT GGAGGAGGGA GGTCCAGGTG AGGCTGGTTT GTCTGCAGAG 
CCTCCCGAGT TGGAAGATTT TGAGGCGACA CTGGGCTCCG ACGGGCGCTG TGGGCGCAGC 
GACGCTTTCA GCAGGTCATC TTCCACCTCC AGCCAGGAGG AGAAGTCATT CCATGCCGAG 
GAGTTGCCTC CTGGGGGCAT CCCCATCGAG CGGGCGGTCT CGGAGGGCTC CCGGGCCTCC 
GTGGACCTCG CCTTCCAGCC CTCTCAGCCC CTGAGCAAGT CCAGCTCGTC GCCTGAACTG 
CAGACCCTGC AGGACATCCT GGGGGACCCC GGGGACAAGG CTGAGGTTGG CCGGCTGAGC 
CCCGAGGCCA AGGCCCGGTC GCAGTCAGGG ATCCTGGACG GGGAAGGTGC CGCCTGGTCA 
GCCCCGGGTG AGGAACGCCG GGGTCGGGGC CCTGCCCAGC CAGAGGGCCC CCTGCCTTCC 
AGCTGTCCTC GCTCCCCCAG CGGGCTGCGG CCCCGAGGCT ACACCATCTC GGATTCGGCC 
CCGTCACGCA GGGGCAAGAG GGTGGAGAGG GATGCCTTCA AGAACCGAGC AGGGACCTCC 
AACACCGAGA AGGTGCCAGG CATCAACCCC AGCTTCGTGT TCCTGCAGCT CTACCACTCA 
CCGTTCTTTG GTGACGAGTC CAACAAGCCA ATCCTTTTGC CTAACGAGTC TTTCGAACGA 
TCAGTGCAGC TCCTTGATCA GATCCCGTCG TACGACACGC ACAAGATCGC GGTCCTGTAC 
GTGGGAGAAG GCCAGAGCAA CAGCGAGCTC GCCATCCTGT CCAACGAGCA TGGCTCCTAC 
AGGTACACAG AGTTCCTCAC GGGGCTGGGC AAGCTCATCG AGCTCAAGGA CTGCCAGCCG 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGCGGCGAGG ATGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCTGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ACCGCTGTGA CAAGAAGCGC CACCTGGGCA ACGACTTCGT GTCCATCGTC 
TACAACGACT CTGGCGAGGA CTTCAAGTTG GGCACCATCA AGGGCCAATT CAACTTCGTC 
CACGTGATTA TCACGCCCCT GGACTACGAG TGCAACCTGG TGTCTCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA TACCAGCATG GCCAAGATCG TGTCTGACCG AAACCTGCCC 
TTCGTGGCCC GCCAGATGGC CCTGCACGCC AATATGGCCT CGCAGGTGCA CCATAGCCGC 
TCCAATCCCA CCGACATTTA TCCCTCCAAG TGGATTGCCA GGCTCCGCCA CATCAAGCGG 
CTGCGCCAGC GGATCCGTGA GGAAGCCCAC TACTCAAGCG CCAGCCTGCC CCTGATGCAG 
ACACACCCTC CTGGTCATGC CAAGGCTCCG GCACAGGCCC CGGCCGAGTC CACGCCCACC 
TATGAGACGG GCCAGCGGAA ACGCCTGGTC TCCTCTGTGG ACGACTTCAC TGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003584053.2
CDS	650..5392
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_003584005.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003584005.4

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGATTCTG CGGTGGTCCT GGAGGAGGGA GGTCCAGGTG AGGCTGGTTT GTCTGCAGAG 
CCTCCCGAGT TGGAAGATTT TGAGGCGACA CTGGGCTCCG ACGGGCGCTG TGGGCGCAGC 
GACGCTTTCA GCAGGTCATC TTCCACCTCC AGCCAGGAGG AGAAGTCATT CCATGCCGAG 
GAGTTGCCTC CTGGGGGCAT CCCCATCGAG CGGGCGGTCT CGGAGGGCTC CCGGGCCTCC 
GTGGACCTCG CCTTCCAGCC CTCTCAGCCC CTGAGCAAGT CCAGCTCGTC GCCTGAACTG 
CAGACCCTGC AGGACATCCT GGGGGACCCC GGGGACAAGG CTGAGGTTGG CCGGCTGAGC 
CCCGAGGCCA AGGCCCGGTC GCAGTCAGGG ATCCTGGACG GGGAAGGTGC CGCCTGGTCA 
GCCCCGGGTG AGGAACGCCG GGGTCGGGGC CCTGCCCAGC CAGAGGGCCC CCTGCCTTCC 
AGCTGTCCTC GCTCCCCCAG CGGGCTGCGG CCCCGAGGCT ACACCATCTC GGATTCGGCC 
CCGTCACGCA GGGGCAAGAG GGTGGAGAGG GATGCCTTCA AGAACCGAGC AGGGACCTCC 
AACACCGAGA AGGTGCCAGG CATCAACCCC AGCTTCGTGT TCCTGCAGCT CTACCACTCA 
CCGTTCTTTG GTGACGAGTC CAACAAGCCA ATCCTTTTGC CTAACGAGTC TTTCGAACGA 
TCAGTGCAGC TCCTTGATCA GATCCCGTCG TACGACACGC ACAAGATCGC GGTCCTGTAC 
GTGGGAGAAG GCCAGAGCAA CAGCGAGCTC GCCATCCTGT CCAACGAGCA TGGCTCCTAC 
AGGTACACAG AGTTCCTCAC GGGGCTGGGC AAGCTCATCG AGCTCAAGGA CTGCCAGCCG 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGCGGCGAGG ATGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCTGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ACCGCTGTGA CAAGAAGCGC CACCTGGGCA ACGACTTCGT GTCCATCGTC 
TACAACGACT CTGGCGAGGA CTTCAAGTTG GGCACCATCA AGGGCCAATT CAACTTCGTC 
CACGTGATTA TCACGCCCCT GGACTACGAG TGCAACCTGG TGTCTCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA TACCAGCATG GCCAAGATCG TGTCTGACCG AAACCTGCCC 
TTCGTGGCCC GCCAGATGGC CCTGCACGCC AATATGGCCT CGCAGGTGCA CCATAGCCGC 
TCCAATCCCA CCGACATTTA TCCCTCCAAG TGGATTGCCA GGCTCCGCCA CATCAAGCGG 
CTGCGCCAGC GGATCCGTGA GGAAGCCCAC TACTCAAGCG CCAGCCTGCC CCTGATGCAG 
ACACACCCTC CTGGTCATGC CAAGGCTCCG GCACAGGCCC CGGCCGAGTC CACGCCCACC 
TATGAGACGG GCCAGCGGAA ACGCCTGGTC TCCTCTGTGG ACGACTTCAC TGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_015460256.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315742.1 Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa88439
Clone ID Related Accession (Same CDS sequence)	XM_003584005.4 , XM_015460256.1
Accession Version	XM_015460256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGATTCTG CGGTGGTCCT GGAGGAGGGA GGTCCAGGTG AGGCTGGTTT GTCTGCAGAG 
CCTCCCGAGT TGGAAGATTT TGAGGCGACA CTGGGCTCCG ACGGGCGCTG TGGGCGCAGC 
GACGCTTTCA GCAGGTCATC TTCCACCTCC AGCCAGGAGG AGAAGTCATT CCATGCCGAG 
GAGTTGCCTC CTGGGGGCAT CCCCATCGAG CGGGCGGTCT CGGAGGGCTC CCGGGCCTCC 
GTGGACCTCG CCTTCCAGCC CTCTCAGCCC CTGAGCAAGT CCAGCTCGTC GCCTGAACTG 
CAGACCCTGC AGGACATCCT GGGGGACCCC GGGGACAAGG CTGAGGTTGG CCGGCTGAGC 
CCCGAGGCCA AGGCCCGGTC GCAGTCAGGG ATCCTGGACG GGGAAGGTGC CGCCTGGTCA 
GCCCCGGGTG AGGAACGCCG GGGTCGGGGC CCTGCCCAGC CAGAGGGCCC CCTGCCTTCC 
AGCTGTCCTC GCTCCCCCAG CGGGCTGCGG CCCCGAGGCT ACACCATCTC GGATTCGGCC 
CCGTCACGCA GGGGCAAGAG GGTGGAGAGG GATGCCTTCA AGAACCGAGC AGGGACCTCC 
AACACCGAGA AGGTGCCAGG CATCAACCCC AGCTTCGTGT TCCTGCAGCT CTACCACTCA 
CCGTTCTTTG GTGACGAGTC CAACAAGCCA ATCCTTTTGC CTAACGAGTC TTTCGAACGA 
TCAGTGCAGC TCCTTGATCA GATCCCGTCG TACGACACGC ACAAGATCGC GGTCCTGTAC 
GTGGGAGAAG GCCAGAGCAA CAGCGAGCTC GCCATCCTGT CCAACGAGCA TGGCTCCTAC 
AGGTACACAG AGTTCCTCAC GGGGCTGGGC AAGCTCATCG AGCTCAAGGA CTGCCAGCCG 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGCGGCGAGG ATGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCTGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ACCGCTGTGA CAAGAAGCGC CACCTGGGCA ACGACTTCGT GTCCATCGTC 
TACAACGACT CTGGCGAGGA CTTCAAGTTG GGCACCATCA AGGGCCAATT CAACTTCGTC 
CACGTGATTA TCACGCCCCT GGACTACGAG TGCAACCTGG TGTCTCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA TACCAGCATG GCCAAGATCG TGTCTGACCG AAACCTGCCC 
TTCGTGGCCC GCCAGATGGC CCTGCACGCC AATATGGCCT CGCAGGTGCA CCATAGCCGC 
TCCAATCCCA CCGACATTTA TCCCTCCAAG TGGATTGCCA GGCTCCGCCA CATCAAGCGG 
CTGCGCCAGC GGATCCGTGA GGAAGCCCAC TACTCAAGCG CCAGCCTGCC CCTGATGCAG 
ACACACCCTC CTGGTCATGC CAAGGCTCCG GCACAGGCCC CGGCCGAGTC CACGCCCACC 
TATGAGACGG GCCAGCGGAA ACGCCTGGTC TCCTCTGTGG ACGACTTCAC TGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315742.1
CDS	650..5392
Translation	MDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSPELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLDIVEKVIARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSEKKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQGGPELSSDPSMHVRQTKEAPAKLESQAGQQVYHGARDRVRSMSGGHGLRVGALDAPACYFPSSPTSPGSQTAPASQPEKASAGSPLPAQKEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINSMPLQELSNALMAAERFKERRDTALYKSLSVPAAGSAKPSPPPRSNTDSAVVLEEGGPGEAGLSAEPPELEDFEATLGSDGRCGRSDAFSRSSSTSSQEEKSFHAEELPPGGIPIERAVSEGSRASVDLAFQPSQPLSKSSSSPELQTLQDILGDPGDKAEVGRLSPEAKARSQSGILDGEGAAWSAPGEERRGRGPAQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEAHYSSASLPLMQTHPPGHAKAPAQAPAESTPTYETGQRKRLVSSVDDFTEFV

Target ORF information:

RefSeq Version	XM_015460256.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tuberous sclerosis 2 (TSC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460256.1

ORF Insert Sequence:

ATGGACGTTG GCCTGTCCTC CGAATTCCTC CTGGTGCTCG TGAACTTAGT CAAATTCAAC 
AGCTGTTACC TTGATGAGTA CATCGCATCC ATGGTCCACA TGGTCTGCCT GCTGTGTGTC 
CAGACCGTGT CCTCCGTGGA CATAGAGGTG TCTCTGCAGG TGCTGGATGC TGTGGTCTGC 
TACAACTGTC TGCCAGCCGA GAGCCTCCCC CTATTCATCG TCACTCTCTG CCGCACCATC 
AACGTCAAGG AGCTCTGTGA ACCTTGCTGG AAGCTGATGC GCAACCTCCT GGGCACCCAC 
CTGGGCCACA GCGCCATCTA CCACATGTGC CGCATCATGG AAGACAGAGC CTACATGGAG 
GACGCACCAC TGCTGAGAGG AGCCGTGTTC TTTGTTGGCA TGGCTCTCTG GGGTGCCCAC 
CGGCTCTATT CTCTCAAGAA CTCCCCAACA TCTGTGTTGC CATCGTTTTA TGAGGCCATG 
ACCTGTCCGA ATGAGGTGGT GTCCTACGAG ATTGTCCTCT CCATAACCAG ACTCATCAAG 
AAGTACAGGA GGGAGCTCCA GGCCGTGACC TGGGACATCC TGCTGAACAT CATCGAGCGC 
CTGCTCCAGC AGCTCCAGAG CCTGGACAGC CCAGAGCTGA GCGCCATCGT GCACGATCTG 
CTGAGCACAG TGGAGGAGCT GTGTGACCAG AACGAGTTTC ACGGCTCCCA GGAGAGATAC 
TTTGAACTCG TGGAGAGGTG TGCGGACCAG AGGCCTGAGT CATCCCTTTT AAACTTAATA 
ACCTACAGGG CTCAGTCCAT CCATCCAGCG AAGGACGGCT GGATCCACAA CCTGCAGCTG 
CTGATGGAGA GGTTTTTCAG GAACGAGTCA CGCAGTGCCG TGCGCATCAA GGTGCTGGAC 
GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTACG AGGAGGAGCT TATCAACTCA 
GTGGTCATCT CGCAGCTCTC CCACATCCCT GAGGATAGAG ACCACCAGGT CCGGAAGCTG 
GCCACTCAGC TGCTGGTAGA TCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGCCTG 
CTGGACATTG TTGAGAAGGT GATCGCCCGT TCCCTCTCTC CGCCCCCCGA GCTGGAGGAG 
AGGGATGTGG CCGCGTACTC GGCCTCCTTG GAGGATGTGA AGACTGCGGT CCTGGGGCTC 
CTGGTCATCC TTCAGACTAA GCTGTATGCC CTGCCTGCCA GCCATGCCAC ACGCGTGTAC 
GAGACGCTGG TCAGCCACAT TCAGCTCCAC TACAGGCATA GCTACACCCT GCCCATCGCC 
AGCAGCATCC GTCTGCAGGC CTTCGACTTC CTGCTGCAGC TGCGGGCCGA CTCACTGCAC 
CGCCTGGGCC TGCCCAGCAA AGACGGGCTT GTGAGGTTCA GCCCCTACTG CGTCTGTGAC 
TGCCTGGAGA CAGAGAGGAG CTCCGAGAAG AAGGCCGGGT GCCCCCTGTC CCCGCCCGCC 
GGGCCCCCTG GCCCTGCGCC TGCGGGCCCT GCCGTGCGCC TTGGGTCCCT GCCCTACTCC 
CTGCTCTTCC GTGTTCTGCT GCAGTGTCTG AAGCAGGAGA CCGACTGGAA GGTGCTGAAG 
CTGGTGCTCA GCAAACTGCC CGAGTCGCTG CGCTATAAGG TCCTCATCTT CACCTCCCCA 
TGCAGTGTCG ACCAGCTGTC CGCCGCCCTC TGCTCCATGC TTTCAGGTCC CAAGACCCTG 
GAGCGGCTCC GAGGCACTCC AGAAGGCTTC TCCAGAACCG ACCTGCATCT CGCTGTGGTT 
CCCGTGCTGA CAGCCCTGAT CTCTTACCAT AACTACTTGG ACAAAACTAG ACAGCGGGAG 
ATGGTGTACT GCCTGGAGCA AGGCCTCATC TACCGCTGTG CCAGCCAGTG TGTGGTGGCC 
CTGGCCGTGT GCAGCGTGGA GATGCCCGAC GTCATCCTCA AGGCACTGCC CGTCCTGGTT 
GTGAAGCTCA CACACATCTC GGCCACGGCC AGCATGGCCA TCCCGCTCCT CGAGTTCCTG 
TCCACTCTGG CCAGACTGCC TCACCTCTAC AGGAACTTCG CAGCAGAGCA GTATGCCAGT 
GTGTTTGCCA TCTCCCTGCC GTATACCAAC CCCTCCAAGT TCAATCAGTA CATCGTGTGC 
CTGGCCCATC ACGTCATAGC CATGTGGTTC ATCAGGTGCC GCCTGCCCTT CCGGAAGGAC 
TTTGTTCCTT ACATCACCAA GGGTCTGCGC TCCAACGTCC TCCTGTCTTT CGATGACACC 
CCCGAGAAGG ACAGCTTCCG AGCGCGGAGC ACCAGTCTCA ACGAGAGGCC CAAGAGGATC 
CAGACCTCTC TCACGAGCGC CAGCTTGGGG TCTGCAGACG AGAACTCGAT GGCCCAGGCC 
GACGACAACT TGAAAAACCT CCACCTGGAG CTCACAGAGA CGTGTCTGGA CATGATGGCC 
AGATATGTGT TCTCCAACTT CACGGCGGTC CCCAAGAGGT CCCCCGTGGG AGAGTTCCTC 
CTGGCTGGTG GCAGGACCAA AACCTGGCTG GTTGGGAACA AGCTTGTCAC CGTGACGACA 
AGCGTGGGGA CCGGGACCCG GTCACTGCTA GGCCTGGACT CTGGAGAGCT GCAGGGTGGC 
CCGGAGTTGA GCTCTGACCC CAGCATGCAT GTGAGACAGA CAAAGGAGGC ACCCGCCAAG 
CTGGAGTCCC AAGCTGGGCA GCAGGTGTAC CACGGAGCCC GGGACCGGGT CCGCTCCATG 
TCCGGGGGCC ATGGCCTTCG CGTGGGTGCC TTGGACGCTC CAGCCTGCTA CTTCCCCAGC 
AGCCCCACGT CCCCGGGCTC CCAGACCGCT CCAGCCAGCC AGCCCGAGAA GGCCTCAGCT 
GGCAGCCCGC TCCCAGCACA GAAGGAGAAG ACAAACCTGG CCGCTTACGT GCCCCTGCTG 
ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC AGGCCCACAG GGAACACCAG CTGGCTGATG 
AGCCTGGAGA ACCCGCTCAG CCCCTTCTCC TCGGACATCA ACAGCATGCC GCTGCAGGAG 
CTGTCCAATG CCCTCATGGC CGCCGAGCGC TTCAAGGAGC GCCGCGACAC AGCCCTGTAC 
AAGTCGCTGT CGGTGCCGGC GGCCGGCTCA GCCAAGCCAT CCCCGCCCCC ACGCTCCAAC 
ACAGATTCTG CGGTGGTCCT GGAGGAGGGA GGTCCAGGTG AGGCTGGTTT GTCTGCAGAG 
CCTCCCGAGT TGGAAGATTT TGAGGCGACA CTGGGCTCCG ACGGGCGCTG TGGGCGCAGC 
GACGCTTTCA GCAGGTCATC TTCCACCTCC AGCCAGGAGG AGAAGTCATT CCATGCCGAG 
GAGTTGCCTC CTGGGGGCAT CCCCATCGAG CGGGCGGTCT CGGAGGGCTC CCGGGCCTCC 
GTGGACCTCG CCTTCCAGCC CTCTCAGCCC CTGAGCAAGT CCAGCTCGTC GCCTGAACTG 
CAGACCCTGC AGGACATCCT GGGGGACCCC GGGGACAAGG CTGAGGTTGG CCGGCTGAGC 
CCCGAGGCCA AGGCCCGGTC GCAGTCAGGG ATCCTGGACG GGGAAGGTGC CGCCTGGTCA 
GCCCCGGGTG AGGAACGCCG GGGTCGGGGC CCTGCCCAGC CAGAGGGCCC CCTGCCTTCC 
AGCTGTCCTC GCTCCCCCAG CGGGCTGCGG CCCCGAGGCT ACACCATCTC GGATTCGGCC 
CCGTCACGCA GGGGCAAGAG GGTGGAGAGG GATGCCTTCA AGAACCGAGC AGGGACCTCC 
AACACCGAGA AGGTGCCAGG CATCAACCCC AGCTTCGTGT TCCTGCAGCT CTACCACTCA 
CCGTTCTTTG GTGACGAGTC CAACAAGCCA ATCCTTTTGC CTAACGAGTC TTTCGAACGA 
TCAGTGCAGC TCCTTGATCA GATCCCGTCG TACGACACGC ACAAGATCGC GGTCCTGTAC 
GTGGGAGAAG GCCAGAGCAA CAGCGAGCTC GCCATCCTGT CCAACGAGCA TGGCTCCTAC 
AGGTACACAG AGTTCCTCAC GGGGCTGGGC AAGCTCATCG AGCTCAAGGA CTGCCAGCCG 
GACAAGGTGT ACCTGGGCGG CCTGGACGTG TGCGGCGAGG ATGGCCAGTT CACCTACTGC 
TGGCACGACG ACATCATGCA AGCTGTCTTC CACATCGCCA CCCTGATGCC CACCAAGGAC 
GTGGACAAGC ACCGCTGTGA CAAGAAGCGC CACCTGGGCA ACGACTTCGT GTCCATCGTC 
TACAACGACT CTGGCGAGGA CTTCAAGTTG GGCACCATCA AGGGCCAATT CAACTTCGTC 
CACGTGATTA TCACGCCCCT GGACTACGAG TGCAACCTGG TGTCTCTGCA GTGCAGGAAA 
GACATGGAGG GCCTTGTGGA TACCAGCATG GCCAAGATCG TGTCTGACCG AAACCTGCCC 
TTCGTGGCCC GCCAGATGGC CCTGCACGCC AATATGGCCT CGCAGGTGCA CCATAGCCGC 
TCCAATCCCA CCGACATTTA TCCCTCCAAG TGGATTGCCA GGCTCCGCCA CATCAAGCGG 
CTGCGCCAGC GGATCCGTGA GGAAGCCCAC TACTCAAGCG CCAGCCTGCC CCTGATGCAG 
ACACACCCTC CTGGTCATGC CAAGGCTCCG GCACAGGCCC CGGCCGAGTC CACGCCCACC 
TATGAGACGG GCCAGCGGAA ACGCCTGGTC TCCTCTGTGG ACGACTTCAC TGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC2
Entrez Gene ID	504985
Full Name	TSC complex subunit 2
General protein information	Preferred Names TSC complex subunit 2 Names tuberin tuberin-like tuberous sclerosis 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	TSC2	XP_523477.3
Mus musculus (house mouse)	Tsc2	NP_001034452.1
Rattus norvegicus (Norway rat)	Tsc2	NP_036812.2
Gallus gallus (chicken)	TSC2	XP_003642168.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011123	XP_309523.4
Canis lupus familiaris (dog)	TSC2	XP_852047.2
Bos taurus (cattle)	TSC2	XP_003587856.2
Drosophila melanogaster (fruit fly)	gig	NP_524177.1
Homo sapiens (human)	TSC2	NP_000539.2
	TSC2	XP_001083256.1
Danio rerio (zebrafish)	tsc2	XP_695912.6
Xenopus tropicalis (tropical clawed frog)	tsc2	NP_001123795.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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