TSC2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_000548.4	NP_000539.2	tuberin isoform 1
XM_011522636.2	XP_011520938.1	tuberin isoform X1
XM_017023615.1	XP_016879104.1	tuberin isoform X3
XM_011522639.2	XP_011520941.1	tuberin isoform X5
NM_001318832.1	NP_001305761.1	tuberin isoform 9
NM_001318827.1	NP_001305756.1	tuberin isoform 6
NM_001318829.1	NP_001305758.1	tuberin isoform 7
NM_001114382.2	NP_001107854.1	tuberin isoform 5
XM_017023616.1	XP_016879105.1	tuberin isoform X9
NM_001077183.2	NP_001070651.1	tuberin isoform 4
XM_011522637.2	XP_011520939.1	tuberin isoform X2
XM_011522640.2	XP_011520942.1	tuberin isoform X6
XM_024450413.1	XP_024306181.1	tuberin isoform X7
NM_001363528.1	NP_001350457.1	tuberin isoform 10
XM_017023617.1	XP_016879106.1	tuberin isoform X11
XM_017023618.1	XP_016879107.1	tuberin isoform X12
NM_000548.5	NP_000539.2	tuberin isoform 1
NM_021055.2	NP_066399.2	tuberin isoform 2
XM_005255529.4	XP_005255586.2	tuberin isoform X7
XM_005255531.4	XP_005255588.2	tuberin isoform X10
NM_001370404.1	NP_001357333.1	tuberin isoform 11
NM_001318831.1	NP_001305760.1	tuberin isoform 8
XM_011522638.2	XP_011520940.2	tuberin isoform X4
NM_001370405.1	NP_001357334.1	tuberin isoform 12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu86243	XM_017023618.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu101801	NM_001318829.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu101912	NM_001318832.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu101969	NM_001318831.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu108136	NM_001370405.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu86241	NM_001370404.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104194	XM_024450413.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu86239	XM_017023615.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu86241	XM_017023616.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu28113	NM_000548.5 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu28168	NM_001114382.2 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu28176	NM_001077183.2 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu61157	NM_021055.2 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61158	NM_001363528.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61159	NM_001318827.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61152	XM_011522636.2 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61153	XM_011522637.2 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61155	XM_011522639.2 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61156	XM_011522640.2 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61157	XM_005255529.4 Latest version!	Homo sapiens tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61158	XM_005255531.4 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu86240	XM_011522638.2 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu86242	XM_017023617.1 Latest version!	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu28113	NM_000548.4	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

TSC2 ( XM_017023618.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879107.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu86243
Clone ID Related Accession (Same CDS sequence)	XM_017023618.1
Accession Version	XM_017023618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAT TCTTCAGGAG CGAGTCCCGA GGCGCCGTGC GCATCAAGGT GCTGGACGTG 
CTGTCCTTTG TGCTGCTCAT CAACAGGCAG TTCTATGAGG AGGAGCTGAT TAACTCAGTG 
GTCATCTCGC AGCTCTCCCA CATCCCCGAG GATAAAGACC ACCAGGTCCG AAAGCTGGCC 
ACCCAGTTGC TGGTGGACCT GGCAGAGGGC TGCCACACAC ACCACTTCAA CAGCCTGCTG 
GACATCATCG AGAAGGTGAT GGCCCGCTCC CTCTCCCCAC CCCCGGAGCT GGAAGAAAGG 
GATGTGGCCG CATACTCGGC CTCCTTGGAG GATGTGAAGA CAGCCGTCCT GGGGCTTCTG 
GTCATCCTTC AGACCAAGCT GTACACCCTG CCTGCAAGCC ACGCCACGCG TGTGTATGAG 
ATGCTGGTCA GCCACATTCA GCTCCACTAC AAGCACAGCT ACACCCTGCC AATCGCGAGC 
AGCATCCGGC TGCAGGCCTT TGACTTCCTG TTGCTGCTGC GGGCCGACTC ACTGCACCGC 
CTGGGCCTGC CCAACAAGGA TGGAGTCGTG CGGTTCAGCC CCTACTGCGT CTGCGACTAC 
ATGGAGCCAG AGAGAGGCTC TGAGAAGAAG ACCAGCGGCC CCCTTTCTCC TCCCACAGGG 
CCTCCTGGCC CGGCGCCTGC AGGCCCCGCC GTGCGGCTGG GGTCCGTGCC CTACTCCCTG 
CTCTTCCGCG TCCTGCTGCA GTGCTTGAAG CAGGAGTCTG ACTGGAAGGT GCTGAAGCTG 
GTTCTGGGCA GGCTGCCTGA GTCCCTGCGC TATAAAGTGC TCATCTTTAC TTCCCCTTGC 
AGTGTGGACC AGCTGTGCTC TGCTCTCTGC TCCATGCTTT CAGGCCCAAA GACACTGGAG 
CGGCTCCGAG GCGCCCCAGA AGGCTTCTCC AGAACTGACT TGCACCTGGC CGTGGTTCCA 
GTGCTGACAG CATTAATCTC TTACCATAAC TACCTGGACA AAACCAAACA GCGCGAGATG 
GTCTACTGCC TGGAGCAGGG CCTCATCCAC CGCTGTGCCA GCCAGTGCGT CGTGGCCTTG 
TCCATCTGCA GCGTGGAGAT GCCTGACATC ATCATCAAGG CGCTGCCTGT TCTGGTGGTG 
AAGCTCACGC ACATCTCAGC CACAGCCAGC ATGGCCGTCC CACTGCTGGA GTTCCTGTCC 
ACTCTGGCCA GGCTGCCGCA CCTCTACAGG AACTTTGCCG CGGAGCAGTA TGCCAGTGTG 
TTCGCCATCT CCCTGCCGTA CACCAACCCC TCCAAGTTTA ATCAGTACAT CGTGTGTCTG 
GCCCATCACG TCATAGCCAT GTGGTTCATC AGGTGCCGCC TGCCCTTCCG GAAGGATTTT 
GTCCCTTTCA TCACTAAGGG CCTGCGGTCC AATGTCCTCT TGTCTTTTGA TGACACCCCC 
GAGAAGGACA GCTTCAGGGC CCGGAGTACT AGTCTCAACG AGAGACCCAA GAGTCTGAGG 
ATAGCCAGAC CCCCCAAACA AGGCTTGAAT AACTCTCCAC CCGTGAAAGA ATTCAAGGAG 
AGCTCTGCAG CCGAGGCCTT CCGGTGCCGC AGCATCAGTG TGTCTGAACA TGTGGTCCGC 
AGCAGGATAC AGACGTCCCT CACCAGTGCC AGCTTGGGGT CTGCAGATGA GAACTCCGTG 
GCCCAGGCTG ACGATAGCCT GAAAAACCTC CACCTGGAGC TCACGGAAAC CTGTCTGGAC 
ATGATGGCTC GATACGTCTT CTCCAACTTC ACGGCTGTCC CGAAGAGGTC TCCTGTGGGC 
GAGTTCCTCC TAGCGGGTGG CAGGACCAAA ACCTGGCTGG TTGGGAACAA GCTTGTCACT 
GTGACGACAA GCGTGGGAAC CGGGACCCGG TCGTTACTAG GCCTGGACTC GGGGGAGCTG 
CAGTCCGGCC CGGAGTCGAG CTCCAGCCCC GGGGTGCATG TGAGACAGAC CAAGGAGGCG 
CCGGCCAAGC TGGAGTCCCA GGCTGGGCAG CAGGTGTCCC GTGGGGCCCG GGATCGGGTC 
CGTTCCATGT CGGGGGGCCA TGGTCTTCGA GTTGGCGCCC TGGACGTGCC GGCCTCCCAG 
TTCCTGGGCA GTGCCACTTC TCCAGGACCA CGGACTGCAC CAGCCGCGAA ACCTGAGAAG 
GCCTCAGCTG GCACCCGGGT TCCTGTGCAG GAGAAGACGA ACCTGGCGGC CTATGTGCCC 
CTGCTGACCC AGGGCTGGGC GGAGATCCTG GTCCGGAGGC CCACAGGGAA CACCAGCTGG 
CTGATGAGCC TGGAGAACCC GCTCAGCCCT TTCTCCTCGG ACATCAACAA CATGCCCCTG 
CAGGAGCTGT CTAACGCCCT CATGGCGGCT GAGCGCTTCA AGGAGCACCG GGACACAGCC 
CTGTACAAGT CACTGTCGGT GCCGGCAGCC AGCACGGCCA AACCCCCTCC TCTGCCTCGC 
TCCAACACAG TGGCCTCTTT CTCCTCCCTG TACCAGTCCA GCTGCCAAGG ACAGCTGCAC 
AGGAGCGTTT CCTGGGCAGG TATCGCCTCT CAGAGGGAAG CGGTTGGCTG CAGAGCGCCA 
CTCTGCCTCA TAGACTCCGC CGTGGTCATG GAGGAGGGAA GTCCGGGCGA GGTTCCTGTG 
CTGGTGGAGC CCCCAGGGTT GGAGGACGTT GAGGCAGCGC TAGGCATGGA CAGGCGCACG 
GATGCCTACA GCAGGTCGTC CTCAGTCTCC AGCCAGGAGG AGAAGTCGCT CCACGCGGAG 
GAGCTGGTTG GCAGGGGCAT CCCCATCGAG CGAGTCGTCT CCTCGGAGGG TGGCCGGCCC 
TCTGTGGACC TCTCCTTCCA GCCCTCGCAG CCCCTGAGCA AGTCCAGCTC CTCTCCCGAG 
CTGCAGACTC TGCAGGACAT CCTCGGGGAC CCTGGGGACA AGGCCGACGT GGGCCGGCTG 
AGCCCTGAGG TTAAGGCCCG GTCACAGTCA GGGACCCTGG ACGGGGAAAG TGCTGCCTGG 
TCGGCCTCGG GCGAAGACAG TCGGGGCCAG CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC 
CGCTCGCCCA GTGGCCTCCG GCCCCGAGGT TACACCATCT CCGACTCGGC CCCATCACGC 
AGGGGCAAGA GAGTAGAGAG GGACGCCTTA AAGAGCAGAG CCACAGCCTC CAATGCAGAG 
AAAGTGCCAG GCATCAACCC CAGTTTCGTG TTCCTGCAGC TCTACCATTC CCCCTTCTTT 
GGCGACGAGT CAAACAAGCC AATCCTGCTG CCCAATGAGT CACAGTCCTT TGAGCGGTCG 
GTGCAGCTCC TCGACCAGAT CCCATCATAC GACACCCACA AGATCGCCGT CCTGTATGTT 
GGAGAAGGCC AGAGCAACAG CGAGCTCGCC ATCCTGTCCA ATGAGCATGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG CCTGGGCCGG CTCATCGAGC TGAAGGACTG CCAGCCGGAC 
AAGGTGTACC TGGGAGGCCT GGACGTGTGT GGTGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGATGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCACC GCTGCGACAA GAAGCGCCAC CTGGGCAACG ACTTTGTGTC CATTGTCTAC 
AATGACTCCG GTGAGGACTT CAAGCTTGGC ACCATCAAGG GCCAGTTCAA CTTTGTCCAC 
GTGATCGTCA CCCCGCTGGA CTACGAGTGC AACCTGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCCTTC 
GTGGCCCGCC AGATGGCCCT GCACGCAAAT ATGGCCTCAC AGGTGCATCA TAGCCGCTCC 
AACCCCACCG ATATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGCGGCTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTACCTCT GGTGCACCCT 
CCGTCCCATA GCAAAGCCCC TGCACAGACT CCAGCCGAGC CCACACCTGG CTATGAGGTG 
GGCCAGCGGA AGCGCCTCAT CTCCTCGGTG GAGGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879107.1
CDS	1519..5652
Translation	MERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWAGIASQREAVGCRAPLCLIDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_017023618.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023618.1

ORF Insert Sequence:

ATGGAGAGAT TCTTCAGGAG CGAGTCCCGA GGCGCCGTGC GCATCAAGGT GCTGGACGTG 
CTGTCCTTTG TGCTGCTCAT CAACAGGCAG TTCTATGAGG AGGAGCTGAT TAACTCAGTG 
GTCATCTCGC AGCTCTCCCA CATCCCCGAG GATAAAGACC ACCAGGTCCG AAAGCTGGCC 
ACCCAGTTGC TGGTGGACCT GGCAGAGGGC TGCCACACAC ACCACTTCAA CAGCCTGCTG 
GACATCATCG AGAAGGTGAT GGCCCGCTCC CTCTCCCCAC CCCCGGAGCT GGAAGAAAGG 
GATGTGGCCG CATACTCGGC CTCCTTGGAG GATGTGAAGA CAGCCGTCCT GGGGCTTCTG 
GTCATCCTTC AGACCAAGCT GTACACCCTG CCTGCAAGCC ACGCCACGCG TGTGTATGAG 
ATGCTGGTCA GCCACATTCA GCTCCACTAC AAGCACAGCT ACACCCTGCC AATCGCGAGC 
AGCATCCGGC TGCAGGCCTT TGACTTCCTG TTGCTGCTGC GGGCCGACTC ACTGCACCGC 
CTGGGCCTGC CCAACAAGGA TGGAGTCGTG CGGTTCAGCC CCTACTGCGT CTGCGACTAC 
ATGGAGCCAG AGAGAGGCTC TGAGAAGAAG ACCAGCGGCC CCCTTTCTCC TCCCACAGGG 
CCTCCTGGCC CGGCGCCTGC AGGCCCCGCC GTGCGGCTGG GGTCCGTGCC CTACTCCCTG 
CTCTTCCGCG TCCTGCTGCA GTGCTTGAAG CAGGAGTCTG ACTGGAAGGT GCTGAAGCTG 
GTTCTGGGCA GGCTGCCTGA GTCCCTGCGC TATAAAGTGC TCATCTTTAC TTCCCCTTGC 
AGTGTGGACC AGCTGTGCTC TGCTCTCTGC TCCATGCTTT CAGGCCCAAA GACACTGGAG 
CGGCTCCGAG GCGCCCCAGA AGGCTTCTCC AGAACTGACT TGCACCTGGC CGTGGTTCCA 
GTGCTGACAG CATTAATCTC TTACCATAAC TACCTGGACA AAACCAAACA GCGCGAGATG 
GTCTACTGCC TGGAGCAGGG CCTCATCCAC CGCTGTGCCA GCCAGTGCGT CGTGGCCTTG 
TCCATCTGCA GCGTGGAGAT GCCTGACATC ATCATCAAGG CGCTGCCTGT TCTGGTGGTG 
AAGCTCACGC ACATCTCAGC CACAGCCAGC ATGGCCGTCC CACTGCTGGA GTTCCTGTCC 
ACTCTGGCCA GGCTGCCGCA CCTCTACAGG AACTTTGCCG CGGAGCAGTA TGCCAGTGTG 
TTCGCCATCT CCCTGCCGTA CACCAACCCC TCCAAGTTTA ATCAGTACAT CGTGTGTCTG 
GCCCATCACG TCATAGCCAT GTGGTTCATC AGGTGCCGCC TGCCCTTCCG GAAGGATTTT 
GTCCCTTTCA TCACTAAGGG CCTGCGGTCC AATGTCCTCT TGTCTTTTGA TGACACCCCC 
GAGAAGGACA GCTTCAGGGC CCGGAGTACT AGTCTCAACG AGAGACCCAA GAGTCTGAGG 
ATAGCCAGAC CCCCCAAACA AGGCTTGAAT AACTCTCCAC CCGTGAAAGA ATTCAAGGAG 
AGCTCTGCAG CCGAGGCCTT CCGGTGCCGC AGCATCAGTG TGTCTGAACA TGTGGTCCGC 
AGCAGGATAC AGACGTCCCT CACCAGTGCC AGCTTGGGGT CTGCAGATGA GAACTCCGTG 
GCCCAGGCTG ACGATAGCCT GAAAAACCTC CACCTGGAGC TCACGGAAAC CTGTCTGGAC 
ATGATGGCTC GATACGTCTT CTCCAACTTC ACGGCTGTCC CGAAGAGGTC TCCTGTGGGC 
GAGTTCCTCC TAGCGGGTGG CAGGACCAAA ACCTGGCTGG TTGGGAACAA GCTTGTCACT 
GTGACGACAA GCGTGGGAAC CGGGACCCGG TCGTTACTAG GCCTGGACTC GGGGGAGCTG 
CAGTCCGGCC CGGAGTCGAG CTCCAGCCCC GGGGTGCATG TGAGACAGAC CAAGGAGGCG 
CCGGCCAAGC TGGAGTCCCA GGCTGGGCAG CAGGTGTCCC GTGGGGCCCG GGATCGGGTC 
CGTTCCATGT CGGGGGGCCA TGGTCTTCGA GTTGGCGCCC TGGACGTGCC GGCCTCCCAG 
TTCCTGGGCA GTGCCACTTC TCCAGGACCA CGGACTGCAC CAGCCGCGAA ACCTGAGAAG 
GCCTCAGCTG GCACCCGGGT TCCTGTGCAG GAGAAGACGA ACCTGGCGGC CTATGTGCCC 
CTGCTGACCC AGGGCTGGGC GGAGATCCTG GTCCGGAGGC CCACAGGGAA CACCAGCTGG 
CTGATGAGCC TGGAGAACCC GCTCAGCCCT TTCTCCTCGG ACATCAACAA CATGCCCCTG 
CAGGAGCTGT CTAACGCCCT CATGGCGGCT GAGCGCTTCA AGGAGCACCG GGACACAGCC 
CTGTACAAGT CACTGTCGGT GCCGGCAGCC AGCACGGCCA AACCCCCTCC TCTGCCTCGC 
TCCAACACAG TGGCCTCTTT CTCCTCCCTG TACCAGTCCA GCTGCCAAGG ACAGCTGCAC 
AGGAGCGTTT CCTGGGCAGG TATCGCCTCT CAGAGGGAAG CGGTTGGCTG CAGAGCGCCA 
CTCTGCCTCA TAGACTCCGC CGTGGTCATG GAGGAGGGAA GTCCGGGCGA GGTTCCTGTG 
CTGGTGGAGC CCCCAGGGTT GGAGGACGTT GAGGCAGCGC TAGGCATGGA CAGGCGCACG 
GATGCCTACA GCAGGTCGTC CTCAGTCTCC AGCCAGGAGG AGAAGTCGCT CCACGCGGAG 
GAGCTGGTTG GCAGGGGCAT CCCCATCGAG CGAGTCGTCT CCTCGGAGGG TGGCCGGCCC 
TCTGTGGACC TCTCCTTCCA GCCCTCGCAG CCCCTGAGCA AGTCCAGCTC CTCTCCCGAG 
CTGCAGACTC TGCAGGACAT CCTCGGGGAC CCTGGGGACA AGGCCGACGT GGGCCGGCTG 
AGCCCTGAGG TTAAGGCCCG GTCACAGTCA GGGACCCTGG ACGGGGAAAG TGCTGCCTGG 
TCGGCCTCGG GCGAAGACAG TCGGGGCCAG CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC 
CGCTCGCCCA GTGGCCTCCG GCCCCGAGGT TACACCATCT CCGACTCGGC CCCATCACGC 
AGGGGCAAGA GAGTAGAGAG GGACGCCTTA AAGAGCAGAG CCACAGCCTC CAATGCAGAG 
AAAGTGCCAG GCATCAACCC CAGTTTCGTG TTCCTGCAGC TCTACCATTC CCCCTTCTTT 
GGCGACGAGT CAAACAAGCC AATCCTGCTG CCCAATGAGT CACAGTCCTT TGAGCGGTCG 
GTGCAGCTCC TCGACCAGAT CCCATCATAC GACACCCACA AGATCGCCGT CCTGTATGTT 
GGAGAAGGCC AGAGCAACAG CGAGCTCGCC ATCCTGTCCA ATGAGCATGG CTCCTACAGG 
TACACGGAGT TCCTGACGGG CCTGGGCCGG CTCATCGAGC TGAAGGACTG CCAGCCGGAC 
AAGGTGTACC TGGGAGGCCT GGACGTGTGT GGTGAGGACG GCCAGTTCAC CTACTGCTGG 
CACGATGACA TCATGCAAGC CGTCTTCCAC ATCGCCACCC TGATGCCCAC CAAGGACGTG 
GACAAGCACC GCTGCGACAA GAAGCGCCAC CTGGGCAACG ACTTTGTGTC CATTGTCTAC 
AATGACTCCG GTGAGGACTT CAAGCTTGGC ACCATCAAGG GCCAGTTCAA CTTTGTCCAC 
GTGATCGTCA CCCCGCTGGA CTACGAGTGC AACCTGGTGT CCCTGCAGTG CAGGAAAGAC 
ATGGAGGGCC TTGTGGACAC CAGCGTGGCC AAGATCGTGT CTGACCGCAA CCTGCCCTTC 
GTGGCCCGCC AGATGGCCCT GCACGCAAAT ATGGCCTCAC AGGTGCATCA TAGCCGCTCC 
AACCCCACCG ATATCTACCC CTCCAAGTGG ATTGCCCGGC TCCGCCACAT CAAGCGGCTC 
CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC TCCAACCCCA GCCTACCTCT GGTGCACCCT 
CCGTCCCATA GCAAAGCCCC TGCACAGACT CCAGCCGAGC CCACACCTGG CTATGAGGTG 
GGCCAGCGGA AGCGCCTCAT CTCCTCGGTG GAGGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001318829.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305758.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu101801
Clone ID Related Accession (Same CDS sequence)	NM_001318829.1
Accession Version	NM_001318829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402106.1, AK295672.1, AK125096.1 and CA427637.1. Transcript Variant: This variant (7) differs in the 5' UTR, uses a downstream start codon and contains multiple coding region differences, compared to variant 1. It encodes isoform 7, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTCGCAAAA 
ACCAAGAAAT TTGAAGAGCA CGCAGTGGAA GCACTCTGGA AGGCGGTCGC GGATCTGTTG 
CAGCCGGAGC GGCCGCTGGA GGCCCGGCAC GCGGTGCTGG CTCTGCTGAA GGCCATCGTG 
CAGGGGCAGG GCGAGCGTTT GGGGGTCCTC AGAGCCCTCT TCTTTAAGGT CATCAAGGAT 
TACCCTTCCA ACGAAGACCT TCACGAAAGG CTGGAGGTTT TCAAGGCCCT CACAGACAAT 
GGGAGACACA TCACCTACTT GGAGGAAGAG CTGGCTGACT TTGTCCTGCA GTGGATGGAT 
GTTGGCTTGT CCTCGGAATT CCTTCTGGTG CTGGTGAACT TGGTCAAATT CAATAGCTGT 
TACCTCGACG AGTACATCGC AAGGATGGTT CAGATGATCT GTCTGCTGTG CGTCCGGACC 
GCGTCCTCTG TGGACATAGA GGTCTCCCTG CAGGTGCTGG ACGCCGTGGT CTGCTACAAC 
TGCCTGCCGG CTGAGAGCCT CCCGCTGTTC ATCGTTACCC TCTGTCGCAC CATCAACGTC 
AAGGAGCTCT GCGAGCCTTG CTGGAAGCTG ATGCGGAACC TCCTTGGCAC CCACCTGGGC 
CACAGCGCCA TCTACAACAT GTGCCACCTC ATGGAGGACA GAGCCTACAT GGAGGACGCG 
CCCCTGCTGA GAGGAGCCGT GTTTTTTGTG GGCATGGCTC TCTGGGGAGC CCACCGGCTC 
TATTCTCTCA GGAACTCGCC GACATCTGTG TTGCCATCAT TTTACCAGGC CATGGCATGT 
CCGAACGAGG TGGTGTCCTA TGAGATCGTC CTGTCCATCA CCAGGCTCAT CAAGAAGTAT 
AGGAAGGAGC TCCAGGTGGT GGCGTGGGAC ATTCTGCTGA ACATCATCGA ACGGCTCCTT 
CAGCAGCTCC AGACCTTGGA CAGCCCGGAG CTCAGGACCA TCGTCCATGA CCTGTTGACC 
ACGGTGGAGG AGCTGTGTGA CCAGAACGAG TTCCACGGGT CTCAGGAGAG ATACTTTGAA 
CTGGTGGAGA GATGTGCGGA CCAGAGGCCT GAGTCCTCCC TCCTGAACCT GATCTCCTAT 
AGAGCGCAGT CCATCCACCC GGCCAAGGAC GGCTGGATTC AGAACCTGCA GGCGCTGATG 
GAGAGATTCT TCAGGAGCGA GTCCCGAGGC GCCGTGCGCA TCAAGGTGCT GGACGTGCTG 
TCCTTTGTGC TGCTCATCAA CAGGCAGTTC TATGAGGAGG AGCTGATTAA CTCAGTGGTC 
ATCTCGCAGC TCTCCCACAT CCCCGAGGAT AAAGACCACC AGGTCCGAAA GCTGGCCACC 
CAGTTGCTGG TGGACCTGGC AGAGGGCTGC CACACACACC ACTTCAACAG CCTGCTGGAC 
ATCATCGAGA AGGTGATGGC CCGCTCCCTC TCCCCACCCC CGGAGCTGGA AGAAAGGGAT 
GTGGCCGCAT ACTCGGCCTC CTTGGAGGAT GTGAAGACAG CCGTCCTGGG GCTTCTGGTC 
ATCCTTCAGA CCAAGCTGTA CACCCTGCCT GCAAGCCACG CCACGCGTGT GTATGAGATG 
CTGGTCAGCC ACATTCAGCT CCACTACAAG CACAGCTACA CCCTGCCAAT CGCGAGCAGC 
ATCCGGCTGC AGGCCTTTGA CTTCCTGTTG CTGCTGCGGG CCGACTCACT GCACCGCCTG 
GGCCTGCCCA ACAAGGATGG AGTCGTGCGG TTCAGCCCCT ACTGCGTCTG CGACTACATG 
GAGCCAGAGA GAGGCTCTGA GAAGAAGACC AGCGGCCCCC TTTCTCCTCC CACAGGGCCT 
CCTGGCCCGG CGCCTGCAGG CCCCGCCGTG CGGCTGGGGT CCGTGCCCTA CTCCCTGCTC 
TTCCGCGTCC TGCTGCAGTG CTTGAAGCAG GAGTCTGACT GGAAGGTGCT GAAGCTGGTT 
CTGGGCAGGC TGCCTGAGTC CCTGCGCTAT AAAGTGCTCA TCTTTACTTC CCCTTGCAGT 
GTGGACCAGC TGTGCTCTGC TCTCTGCTCC ATGCTTTCAG GCCCAAAGAC ACTGGAGCGG 
CTCCGAGGCG CCCCAGAAGG CTTCTCCAGA ACTGACTTGC ACCTGGCCGT GGTTCCAGTG 
CTGACAGCAT TAATCTCTTA CCATAACTAC CTGGACAAAA CCAAACAGCG CGAGATGGTC 
TACTGCCTGG AGCAGGGCCT CATCCACCGC TGTGCCAGCC AGTGCGTCGT GGCCTTGTCC 
ATCTGCAGCG TGGAGATGCC TGACATCATC ATCAAGGCGC TGCCTGTTCT GGTGGTGAAG 
CTCACGCACA TCTCAGCCAC AGCCAGCATG GCCGTCCCAC TGCTGGAGTT CCTGTCCACT 
CTGGCCAGGC TGCCGCACCT CTACAGGAAC TTTGCCGCGG AGCAGTATGC CAGTGTGTTC 
GCCATCTCCC TGCCGTACAC CAACCCCTCC AAGTTTAATC AGTACATCGT GTGTCTGGCC 
CATCACGTCA TAGCCATGTG GTTCATCAGG TGCCGCCTGC CCTTCCGGAA GGATTTTGTC 
CCTTTCATCA CTAAGGGCCT GCGGTCCAAT GTCCTCTTGT CTTTTGATGA CACCCCCGAG 
AAGGACAGCT TCAGGGCCCG GAGTACTAGT CTCAACGAGA GACCCAAGAG CAGGATACAG 
ACGTCCCTCA CCAGTGCCAG CTTGGGGTCT GCAGATGAGA ACTCCGTGGC CCAGGCTGAC 
GATAGCCTGA AAAACCTCCA CCTGGAGCTC ACGGAAACCT GTCTGGACAT GATGGCTCGA 
TACGTCTTCT CCAACTTCAC GGCTGTCCCG AAGAGGTCTC CTGTGGGCGA GTTCCTCCTA 
GCGGGTGGCA GGACCAAAAC CTGGCTGGTT GGGAACAAGC TTGTCACTGT GACGACAAGC 
GTGGGAACCG GGACCCGGTC GTTACTAGGC CTGGACTCGG GGGAGCTGCA GTCCGGCCCG 
GAGTCGAGCT CCAGCCCCGG GGTGCATGTG AGACAGACCA AGGAGGCGCC GGCCAAGCTG 
GAGTCCCAGG CTGGGCAGCA GGTGTCCCGT GGGGCCCGGG ATCGGGTCCG TTCCATGTCG 
GGGGGCCATG GTCTTCGAGT TGGCGCCCTG GACGTGCCGG CCTCCCAGTT CCTGGGCAGT 
GCCACTTCTC CAGGACCACG GACTGCACCA GCCGCGAAAC CTGAGAAGGC CTCAGCTGGC 
ACCCGGGTTC CTGTGCAGGA GAAGACGAAC CTGGCGGCCT ATGTGCCCCT GCTGACCCAG 
GGCTGGGCGG AGATCCTGGT CCGGAGGCCC ACAGGGAACA CCAGCTGGCT GATGAGCCTG 
GAGAACCCGC TCAGCCCTTT CTCCTCGGAC ATCAACAACA TGCCCCTGCA GGAGCTGTCT 
AACGCCCTCA TGGCGGCTGA GCGCTTCAAG GAGCACCGGG ACACAGCCCT GTACAAGTCA 
CTGTCGGTGC CGGCAGCCAG CACGGCCAAA CCCCCTCCTC TGCCTCGCTC CAACACAGAC 
TCCGCCGTGG TCATGGAGGA GGGAAGTCCG GGCGAGGTTC CTGTGCTGGT GGAGCCCCCA 
GGGTTGGAGG ACGTTGAGGC AGCGCTAGGC ATGGACAGGC GCACGGATGC CTACAGCAGG 
TCGTCCTCAG TCTCCAGCCA GGAGGAGAAG TCGCTCCACG CGGAGGAGCT GGTTGGCAGG 
GGCATCCCCA TCGAGCGAGT CGTCTCCTCG GAGGGTGGCC GGCCCTCTGT GGACCTCTCC 
TTCCAGCCCT CGCAGCCCCT GAGCAAGTCC AGCTCCTCTC CCGAGCTGCA GACTCTGCAG 
GACATCCTCG GGGACCCTGG GGACAAGGCC GACGTGGGCC GGCTGAGCCC TGAGGTTAAG 
GCCCGGTCAC AGTCAGGGAC CCTGGACGGG GAAAGTGCTG CCTGGTCGGC CTCGGGCGAA 
GACAGTCGGG GCCAGCCCGA GGGTCCCTTG CCTTCCAGCT CCCCCCGCTC GCCCAGTGGC 
CTCCGGCCCC GAGGTTACAC CATCTCCGAC TCGGCCCCAT CACGCAGGGG CAAGAGAGTA 
GAGAGGGACG CCTTAAAGAG CAGAGCCACA GCCTCCAATG CAGAGAAAGT GCCAGGCATC 
AACCCCAGTT TCGTGTTCCT GCAGCTCTAC CATTCCCCCT TCTTTGGCGA CGAGTCAAAC 
AAGCCAATCC TGCTGCCCAA TGAGTCACAG TCCTTTGAGC GGTCGGTGCA GCTCCTCGAC 
CAGATCCCAT CATACGACAC CCACAAGATC GCCGTCCTGT ATGTTGGAGA AGGCCAGAGC 
AACAGCGAGC TCGCCATCCT GTCCAATGAG CATGGCTCCT ACAGGTACAC GGAGTTCCTG 
ACGGGCCTGG GCCGGCTCAT CGAGCTGAAG GACTGCCAGC CGGACAAGGT GTACCTGGGA 
GGCCTGGACG TGTGTGGTGA GGACGGCCAG TTCACCTACT GCTGGCACGA TGACATCATG 
CAAGCCGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGC 
GACAAGAAGC GCCACCTGGG CAACGACTTT GTGTCCATTG TCTACAATGA CTCCGGTGAG 
GACTTCAAGC TTGGCACCAT CAAGGGCCAG TTCAACTTTG TCCACGTGAT CGTCACCCCG 
CTGGACTACG AGTGCAACCT GGTGTCCCTG CAGTGCAGGA AAGACATGGA GGGCCTTGTG 
GACACCAGCG TGGCCAAGAT CGTGTCTGAC CGCAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCACG CAAATATGGC CTCACAGGTG CATCATAGCC GCTCCAACCC CACCGATATC 
TACCCCTCCA AGTGGATTGC CCGGCTCCGC CACATCAAGC GGCTCCGCCA GCGGATCTGC 
GAGGAAGCCG CCTACTCCAA CCCCAGCCTA CCTCTGGTGC ACCCTCCGTC CCATAGCAAA 
GCCCCTGCAC AGACTCCAGC CGAGCCCACA CCTGGCTATG AGGTGGGCCA GCGGAAGCGC 
CTCATCTCCT CGGTGGAGGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305758.1
CDS	181..5259
Translation	MECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001318829.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318829.1

ORF Insert Sequence:

ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTCGCAAAA 
ACCAAGAAAT TTGAAGAGCA CGCAGTGGAA GCACTCTGGA AGGCGGTCGC GGATCTGTTG 
CAGCCGGAGC GGCCGCTGGA GGCCCGGCAC GCGGTGCTGG CTCTGCTGAA GGCCATCGTG 
CAGGGGCAGG GCGAGCGTTT GGGGGTCCTC AGAGCCCTCT TCTTTAAGGT CATCAAGGAT 
TACCCTTCCA ACGAAGACCT TCACGAAAGG CTGGAGGTTT TCAAGGCCCT CACAGACAAT 
GGGAGACACA TCACCTACTT GGAGGAAGAG CTGGCTGACT TTGTCCTGCA GTGGATGGAT 
GTTGGCTTGT CCTCGGAATT CCTTCTGGTG CTGGTGAACT TGGTCAAATT CAATAGCTGT 
TACCTCGACG AGTACATCGC AAGGATGGTT CAGATGATCT GTCTGCTGTG CGTCCGGACC 
GCGTCCTCTG TGGACATAGA GGTCTCCCTG CAGGTGCTGG ACGCCGTGGT CTGCTACAAC 
TGCCTGCCGG CTGAGAGCCT CCCGCTGTTC ATCGTTACCC TCTGTCGCAC CATCAACGTC 
AAGGAGCTCT GCGAGCCTTG CTGGAAGCTG ATGCGGAACC TCCTTGGCAC CCACCTGGGC 
CACAGCGCCA TCTACAACAT GTGCCACCTC ATGGAGGACA GAGCCTACAT GGAGGACGCG 
CCCCTGCTGA GAGGAGCCGT GTTTTTTGTG GGCATGGCTC TCTGGGGAGC CCACCGGCTC 
TATTCTCTCA GGAACTCGCC GACATCTGTG TTGCCATCAT TTTACCAGGC CATGGCATGT 
CCGAACGAGG TGGTGTCCTA TGAGATCGTC CTGTCCATCA CCAGGCTCAT CAAGAAGTAT 
AGGAAGGAGC TCCAGGTGGT GGCGTGGGAC ATTCTGCTGA ACATCATCGA ACGGCTCCTT 
CAGCAGCTCC AGACCTTGGA CAGCCCGGAG CTCAGGACCA TCGTCCATGA CCTGTTGACC 
ACGGTGGAGG AGCTGTGTGA CCAGAACGAG TTCCACGGGT CTCAGGAGAG ATACTTTGAA 
CTGGTGGAGA GATGTGCGGA CCAGAGGCCT GAGTCCTCCC TCCTGAACCT GATCTCCTAT 
AGAGCGCAGT CCATCCACCC GGCCAAGGAC GGCTGGATTC AGAACCTGCA GGCGCTGATG 
GAGAGATTCT TCAGGAGCGA GTCCCGAGGC GCCGTGCGCA TCAAGGTGCT GGACGTGCTG 
TCCTTTGTGC TGCTCATCAA CAGGCAGTTC TATGAGGAGG AGCTGATTAA CTCAGTGGTC 
ATCTCGCAGC TCTCCCACAT CCCCGAGGAT AAAGACCACC AGGTCCGAAA GCTGGCCACC 
CAGTTGCTGG TGGACCTGGC AGAGGGCTGC CACACACACC ACTTCAACAG CCTGCTGGAC 
ATCATCGAGA AGGTGATGGC CCGCTCCCTC TCCCCACCCC CGGAGCTGGA AGAAAGGGAT 
GTGGCCGCAT ACTCGGCCTC CTTGGAGGAT GTGAAGACAG CCGTCCTGGG GCTTCTGGTC 
ATCCTTCAGA CCAAGCTGTA CACCCTGCCT GCAAGCCACG CCACGCGTGT GTATGAGATG 
CTGGTCAGCC ACATTCAGCT CCACTACAAG CACAGCTACA CCCTGCCAAT CGCGAGCAGC 
ATCCGGCTGC AGGCCTTTGA CTTCCTGTTG CTGCTGCGGG CCGACTCACT GCACCGCCTG 
GGCCTGCCCA ACAAGGATGG AGTCGTGCGG TTCAGCCCCT ACTGCGTCTG CGACTACATG 
GAGCCAGAGA GAGGCTCTGA GAAGAAGACC AGCGGCCCCC TTTCTCCTCC CACAGGGCCT 
CCTGGCCCGG CGCCTGCAGG CCCCGCCGTG CGGCTGGGGT CCGTGCCCTA CTCCCTGCTC 
TTCCGCGTCC TGCTGCAGTG CTTGAAGCAG GAGTCTGACT GGAAGGTGCT GAAGCTGGTT 
CTGGGCAGGC TGCCTGAGTC CCTGCGCTAT AAAGTGCTCA TCTTTACTTC CCCTTGCAGT 
GTGGACCAGC TGTGCTCTGC TCTCTGCTCC ATGCTTTCAG GCCCAAAGAC ACTGGAGCGG 
CTCCGAGGCG CCCCAGAAGG CTTCTCCAGA ACTGACTTGC ACCTGGCCGT GGTTCCAGTG 
CTGACAGCAT TAATCTCTTA CCATAACTAC CTGGACAAAA CCAAACAGCG CGAGATGGTC 
TACTGCCTGG AGCAGGGCCT CATCCACCGC TGTGCCAGCC AGTGCGTCGT GGCCTTGTCC 
ATCTGCAGCG TGGAGATGCC TGACATCATC ATCAAGGCGC TGCCTGTTCT GGTGGTGAAG 
CTCACGCACA TCTCAGCCAC AGCCAGCATG GCCGTCCCAC TGCTGGAGTT CCTGTCCACT 
CTGGCCAGGC TGCCGCACCT CTACAGGAAC TTTGCCGCGG AGCAGTATGC CAGTGTGTTC 
GCCATCTCCC TGCCGTACAC CAACCCCTCC AAGTTTAATC AGTACATCGT GTGTCTGGCC 
CATCACGTCA TAGCCATGTG GTTCATCAGG TGCCGCCTGC CCTTCCGGAA GGATTTTGTC 
CCTTTCATCA CTAAGGGCCT GCGGTCCAAT GTCCTCTTGT CTTTTGATGA CACCCCCGAG 
AAGGACAGCT TCAGGGCCCG GAGTACTAGT CTCAACGAGA GACCCAAGAG CAGGATACAG 
ACGTCCCTCA CCAGTGCCAG CTTGGGGTCT GCAGATGAGA ACTCCGTGGC CCAGGCTGAC 
GATAGCCTGA AAAACCTCCA CCTGGAGCTC ACGGAAACCT GTCTGGACAT GATGGCTCGA 
TACGTCTTCT CCAACTTCAC GGCTGTCCCG AAGAGGTCTC CTGTGGGCGA GTTCCTCCTA 
GCGGGTGGCA GGACCAAAAC CTGGCTGGTT GGGAACAAGC TTGTCACTGT GACGACAAGC 
GTGGGAACCG GGACCCGGTC GTTACTAGGC CTGGACTCGG GGGAGCTGCA GTCCGGCCCG 
GAGTCGAGCT CCAGCCCCGG GGTGCATGTG AGACAGACCA AGGAGGCGCC GGCCAAGCTG 
GAGTCCCAGG CTGGGCAGCA GGTGTCCCGT GGGGCCCGGG ATCGGGTCCG TTCCATGTCG 
GGGGGCCATG GTCTTCGAGT TGGCGCCCTG GACGTGCCGG CCTCCCAGTT CCTGGGCAGT 
GCCACTTCTC CAGGACCACG GACTGCACCA GCCGCGAAAC CTGAGAAGGC CTCAGCTGGC 
ACCCGGGTTC CTGTGCAGGA GAAGACGAAC CTGGCGGCCT ATGTGCCCCT GCTGACCCAG 
GGCTGGGCGG AGATCCTGGT CCGGAGGCCC ACAGGGAACA CCAGCTGGCT GATGAGCCTG 
GAGAACCCGC TCAGCCCTTT CTCCTCGGAC ATCAACAACA TGCCCCTGCA GGAGCTGTCT 
AACGCCCTCA TGGCGGCTGA GCGCTTCAAG GAGCACCGGG ACACAGCCCT GTACAAGTCA 
CTGTCGGTGC CGGCAGCCAG CACGGCCAAA CCCCCTCCTC TGCCTCGCTC CAACACAGAC 
TCCGCCGTGG TCATGGAGGA GGGAAGTCCG GGCGAGGTTC CTGTGCTGGT GGAGCCCCCA 
GGGTTGGAGG ACGTTGAGGC AGCGCTAGGC ATGGACAGGC GCACGGATGC CTACAGCAGG 
TCGTCCTCAG TCTCCAGCCA GGAGGAGAAG TCGCTCCACG CGGAGGAGCT GGTTGGCAGG 
GGCATCCCCA TCGAGCGAGT CGTCTCCTCG GAGGGTGGCC GGCCCTCTGT GGACCTCTCC 
TTCCAGCCCT CGCAGCCCCT GAGCAAGTCC AGCTCCTCTC CCGAGCTGCA GACTCTGCAG 
GACATCCTCG GGGACCCTGG GGACAAGGCC GACGTGGGCC GGCTGAGCCC TGAGGTTAAG 
GCCCGGTCAC AGTCAGGGAC CCTGGACGGG GAAAGTGCTG CCTGGTCGGC CTCGGGCGAA 
GACAGTCGGG GCCAGCCCGA GGGTCCCTTG CCTTCCAGCT CCCCCCGCTC GCCCAGTGGC 
CTCCGGCCCC GAGGTTACAC CATCTCCGAC TCGGCCCCAT CACGCAGGGG CAAGAGAGTA 
GAGAGGGACG CCTTAAAGAG CAGAGCCACA GCCTCCAATG CAGAGAAAGT GCCAGGCATC 
AACCCCAGTT TCGTGTTCCT GCAGCTCTAC CATTCCCCCT TCTTTGGCGA CGAGTCAAAC 
AAGCCAATCC TGCTGCCCAA TGAGTCACAG TCCTTTGAGC GGTCGGTGCA GCTCCTCGAC 
CAGATCCCAT CATACGACAC CCACAAGATC GCCGTCCTGT ATGTTGGAGA AGGCCAGAGC 
AACAGCGAGC TCGCCATCCT GTCCAATGAG CATGGCTCCT ACAGGTACAC GGAGTTCCTG 
ACGGGCCTGG GCCGGCTCAT CGAGCTGAAG GACTGCCAGC CGGACAAGGT GTACCTGGGA 
GGCCTGGACG TGTGTGGTGA GGACGGCCAG TTCACCTACT GCTGGCACGA TGACATCATG 
CAAGCCGTCT TCCACATCGC CACCCTGATG CCCACCAAGG ACGTGGACAA GCACCGCTGC 
GACAAGAAGC GCCACCTGGG CAACGACTTT GTGTCCATTG TCTACAATGA CTCCGGTGAG 
GACTTCAAGC TTGGCACCAT CAAGGGCCAG TTCAACTTTG TCCACGTGAT CGTCACCCCG 
CTGGACTACG AGTGCAACCT GGTGTCCCTG CAGTGCAGGA AAGACATGGA GGGCCTTGTG 
GACACCAGCG TGGCCAAGAT CGTGTCTGAC CGCAACCTGC CCTTCGTGGC CCGCCAGATG 
GCCCTGCACG CAAATATGGC CTCACAGGTG CATCATAGCC GCTCCAACCC CACCGATATC 
TACCCCTCCA AGTGGATTGC CCGGCTCCGC CACATCAAGC GGCTCCGCCA GCGGATCTGC 
GAGGAAGCCG CCTACTCCAA CCCCAGCCTA CCTCTGGTGC ACCCTCCGTC CCATAGCAAA 
GCCCCTGCAC AGACTCCAGC CGAGCCCACA CCTGGCTATG AGGTGGGCCA GCGGAAGCGC 
CTCATCTCCT CGGTGGAGGA CTTCACCGAG TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001318832.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305761.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 9, mRNA.

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CloneID	OHu101912
Clone ID Related Accession (Same CDS sequence)	NM_001318832.1
Accession Version	NM_001318832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK295728.1, AK295672.1 and CA427637.1. Transcript Variant: This variant (9) differs in the 5' UTR, uses an alternate start codon and contains multiple coding region differences, compared to variant 1. It encodes isoform 9, which is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295728.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGT TTTCTGGTGC GTCCTGGTCC ACCATGGCCA AACCAACAAG CAAAGATTCA 
GGCTTGAAGG AGAAGTTTAA GATTCTGTTG GGACTGGGAA CACCGAGGCC AAATCCCAGG 
TCTGCAGAGG GTAAACAGAC GGAGTTTATC ATCACCGCGG AAATACTGAG AGAACTGAGC 
ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTCGCAAAA 
ACCAAGAAAT TTGAAGAGCA CGCAGTGGAA GCACTCTGGA AGGCGGTCGC GGATCTGTTG 
CAGCCGGAGC GGCCGCTGGA GGCCCGGCAC GCGGTGCTGG CTCTGCTGAA GGCCATCGTG 
CAGGGGCAGG GCGAGCGTTT GGGGGTCCTC AGAGCCCTCT TCTTTAAGGT CATCAAGGAT 
TACCCTTCCA ACGAAGACCT TCACGAAAGG CTGGAGGTTT TCAAGGCCCT CACAGACAAT 
GGGAGACACA TCACCTACTT GGAGGAAGAG CTGGCTGACT TTGTCCTGCA GTGGATGGAT 
GTTGGCTTGT CCTCGGAATT CCTTCTGGTG CTGGTGAACT TGGTCAAATT CAATAGCTGT 
TACCTCGACG AGTACATCGC AAGGATGGTT CAGATGATCT GTCTGCTGTG CGTCCGGACC 
GCGTCCTCTG TGGACATAGA GGTCTCCCTG CAGGTGCTGG ACGCCGTGGT CTGCTACAAC 
TGCCTGCCGG CTGAGAGCCT CCCGCTGTTC ATCGTTACCC TCTGTCGCAC CATCAACGTC 
AAGGAGCTCT GCGAGCCTTG CTGGAAGCTG ATGCGGAACC TCCTTGGCAC CCACCTGGGC 
CACAGCGCCA TCTACAACAT GTGCCACCTC ATGGAGGACA GAGCCTACAT GGAGGACGCG 
CCCCTGCTGA GAGGAGCCGT GTTTTTTGTG GGCATGGCTC TCTGGGGAGC CCACCGGCTC 
TATTCTCTCA GGAACTCGCC GACATCTGTG TTGCCATCAT TTTACCAGGC CATGGCATGT 
CCGAACGAGG TGGTGTCCTA TGAGATCGTC CTGTCCATCA CCAGGCTCAT CAAGAAGTAT 
AGGAAGGAGC TCCAGGTGGT GGCGTGGGAC ATTCTGCTGA ACATCATCGA ACGGCTCCTT 
CAGCAGCTCC AGACCTTGGA CAGCCCGGAG CTCAGGACCA TCGTCCATGA CCTGTTGACC 
ACGGTGGAGG AGCTGTGTGA CCAGAACGAG TTCCACGGGT CTCAGGAGAG ATACTTTGAA 
CTGGTGGAGA GATGTGCGGA CCAGAGGCCT GAGTCCTCCC TCCTGAACCT GATCTCCTAT 
AGAGCGCAGT CCATCCACCC GGCCAAGGAC GGCTGGATTC AGAACCTGCA GGCGCTGATG 
GAGAGATTCT TCAGGAGCGA GTCCCGAGGC GCCGTGCGCA TCAAGGTGCT GGACGTGCTG 
TCCTTTGTGC TGCTCATCAA CAGGCAGTTC TATGAGGAGG AGCTGATTAA CTCAGTGGTC 
ATCTCGCAGC TCTCCCACAT CCCCGAGGAT AAAGACCACC AGGTCCGAAA GCTGGCCACC 
CAGTTGCTGG TGGACCTGGC AGAGGGCTGC CACACACACC ACTTCAACAG CCTGCTGGAC 
ATCATCGAGA AGGTGATGGC CCGCTCCCTC TCCCCACCCC CGGAGCTGGA AGAAAGGGAT 
GTGGCCGCAT ACTCGGCCTC CTTGGAGGAT GTGAAGACAG CCGTCCTGGG GCTTCTGGTC 
ATCCTTCAGA CCAAGCTGTA CACCCTGCCT GCAAGCCACG CCACGCGTGT GTATGAGATG 
CTGGTCAGCC ACATTCAGCT CCACTACAAG CACAGCTACA CCCTGCCAAT CGCGAGCAGC 
ATCCGGCTGC AGGCCTTTGA CTTCCTGTTG CTGCTGCGGG CCGACTCACT GCACCGCCTG 
GGCCTGCCCA ACAAGGATGG AGTCGTGCGG TTCAGCCCCT ACTGCGTCTG CGACTACATG 
GAGCCAGAGA GAGGCTCTGA GAAGAAGACC AGCGGCCCCC TTTCTCCTCC CACAGGGCCT 
CCTGGCCCGG CGCCTGCAGG CCCCGCCGTG CGGCTGGGGT CCGTGCCCTA CTCCCTGCTC 
TTCCGCGTCC TGCTGCAGTG CTTGAAGCAG GAGTCTGACT GGAAGGTGCT GAAGCTGGTT 
CTGGGCAGGC TGCCTGAGTC CCTGCGCTAT AAAGTGCTCA TCTTTACTTC CCCTTGCAGT 
GTGGACCAGC TGTGCTCTGC TCTCTGCTCC ATGCTTTCAG GCCCAAAGAC ACTGGAGCGG 
CTCCGAGGCG CCCCAGAAGG CTTCTCCAGA ACTGACTTGC ACCTGGCCGT GGTTCCAGTG 
CTGACAGCAT TAATCTCTTA CCATAACTAC CTGGACAAAA CCAAACAGCG CGAGATGGTC 
TACTGCCTGG AGCAGGGCCT CATCCACCGC TGTGCCAGCC AGTGCGTCGT GGCCTTGTCC 
ATCTGCAGCG TGGAGATGCC TGACATCATC ATCAAGGCGC TGCCTGTTCT GGTGGTGAAG 
CTCACGCACA TCTCAGCCAC AGCCAGCATG GCCGTCCCAC TGCTGGAGTT CCTGTCCACT 
CTGGCCAGGC TGCCGCACCT CTACAGGAAC TTTGCCGCGG AGCAGTATGC CAGTGTGTTC 
GCCATCTCCC TGCCGTACAC CAACCCCTCC AAGTTTAATC AGTACATCGT GTGTCTGGCC 
CATCACGTCA TAGCCATGTG GTTCATCAGG TGCCGCCTGC CCTTCCGGAA GGATTTTGTC 
CCTTTCATCA CTAAGGGCCT GCGGTCCAAT GTCCTCTTGT CTTTTGATGA CACCCCCGAG 
AAGGACAGCT TCAGGGCCCG GAGTACTAGT CTCAACGAGA GACCCAAGAG GATACAGACG 
TCCCTCACCA GTGCCAGCTT GGGGTCTGCA GATGAGAACT CCGTGGCCCA GGCTGACGAT 
AGCCTGAAAA ACCTCCACCT GGAGCTCACG GAAACCTGTC TGGACATGAT GGCTCGATAC 
GTCTTCTCCA ACTTCACGGC TGTCCCGAAG AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG 
GGTGGCAGGA CCAAAACCTG GCTGGTTGGG AACAAGCTTG TCACTGTGAC GACAAGCGTG 
GGAACCGGGA CCCGGTCGTT ACTAGGCCTG GACTCGGGGG AGCTGCAGTC CGGCCCGGAG 
TCGAGCTCCA GCCCCGGGGT GCATGTGAGA CAGACCAAGG AGGCGCCGGC CAAGCTGGAG 
TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG GCCCGGGATC GGGTCCGTTC CATGTCGGGG 
GGCCATGGTC TTCGAGTTGG CGCCCTGGAC GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC 
ACTTCTCCAG GACCACGGAC TGCACCAGCC GCGAAACCTG AGAAGGCCTC AGCTGGCACC 
CGGGTTCCTG TGCAGGAGAA GACGAACCTG GCGGCCTATG TGCCCCTGCT GACCCAGGGC 
TGGGCGGAGA TCCTGGTCCG GAGGCCCACA GGGAACACCA GCTGGCTGAT GAGCCTGGAG 
AACCCGCTCA GCCCTTTCTC CTCGGACATC AACAACATGC CCCTGCAGGA GCTGTCTAAC 
GCCCTCATGG CGGCTGAGCG CTTCAAGGAG CACCGGGACA CAGCCCTGTA CAAGTCACTG 
TCGGTGCCGG CAGCCAGCAC GGCCAAACCC CCTCCTCTGC CTCGCTCCAA CACAGACTCC 
GCCGTGGTCA TGGAGGAGGG AAGTCCGGGC GAGGTTCCTG TGCTGGTGGA GCCCCCAGGG 
TTGGAGGACG TTGAGGCAGC GCTAGGCATG GACAGGCGCA CGGATGCCTA CAGCAGGTCG 
TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG CTCCACGCGG AGGAGCTGGT TGGCAGGGGC 
ATCCCCATCG AGCGAGTCGT CTCCTCGGAG GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC 
CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC 
ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC 
CGGTCACAGT CAGGGACCCT GGACGGGGAA AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC 
AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC 
CGGCCCCGAG GTTACACCAT CTCCGACTCG GCCCCATCAC GCAGGGGCAA GAGAGTAGAG 
AGGGACGCCT TAAAGAGCAG AGCCACAGCC TCCAATGCAG AGAAAGTGCC AGGCATCAAC 
CCCAGTTTCG TGTTCCTGCA GCTCTACCAT TCCCCCTTCT TTGGCGACGA GTCAAACAAG 
CCAATCCTGC TGCCCAATGA GTCACAGTCC TTTGAGCGGT CGGTGCAGCT CCTCGACCAG 
ATCCCATCAT ACGACACCCA CAAGATCGCC GTCCTGTATG TTGGAGAAGG CCAGAGCAAC 
AGCGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCCTACA GGTACACGGA GTTCCTGACG 
GGCCTGGGCC GGCTCATCGA GCTGAAGGAC TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC 
CTGGACGTGT GTGGTGAGGA CGGCCAGTTC ACCTACTGCT GGCACGATGA CATCATGCAA 
GCCGTCTTCC ACATCGCCAC CCTGATGCCC ACCAAGGACG TGGACAAGCA CCGCTGCGAC 
AAGAAGCGCC ACCTGGGCAA CGACTTTGTG TCCATTGTCT ACAATGACTC CGGTGAGGAC 
TTCAAGCTTG GCACCATCAA GGGCCAGTTC AACTTTGTCC ACGTGATCGT CACCCCGCTG 
GACTACGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAGGG CCTTGTGGAC 
ACCAGCGTGG CCAAGATCGT GTCTGACCGC AACCTGCCCT TCGTGGCCCG CCAGATGGCC 
CTGCACGCAA ATATGGCCTC ACAGGTGCAT CATAGCCGCT CCAACCCCAC CGATATCTAC 
CCCTCCAAGT GGATTGCCCG GCTCCGCCAC ATCAAGCGGC TCCGCCAGCG GATCTGCGAG 
GAAGCCGCCT ACTCCAACCC CAGCCTACCT CTGGTGCACC CTCCGTCCCA TAGCAAAGCC 
CCTGCACAGA CTCCAGCCGA GCCCACACCT GGCTATGAGG TGGGCCAGCG GAAGCGCCTC 
ATCTCCTCGG TGGAGGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305761.1
CDS	69..5324
Translation	MEGFSGASWSTMAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001318832.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318832.1

ORF Insert Sequence:

ATGGAGGGGT TTTCTGGTGC GTCCTGGTCC ACCATGGCCA AACCAACAAG CAAAGATTCA 
GGCTTGAAGG AGAAGTTTAA GATTCTGTTG GGACTGGGAA CACCGAGGCC AAATCCCAGG 
TCTGCAGAGG GTAAACAGAC GGAGTTTATC ATCACCGCGG AAATACTGAG AGAACTGAGC 
ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTCGCAAAA 
ACCAAGAAAT TTGAAGAGCA CGCAGTGGAA GCACTCTGGA AGGCGGTCGC GGATCTGTTG 
CAGCCGGAGC GGCCGCTGGA GGCCCGGCAC GCGGTGCTGG CTCTGCTGAA GGCCATCGTG 
CAGGGGCAGG GCGAGCGTTT GGGGGTCCTC AGAGCCCTCT TCTTTAAGGT CATCAAGGAT 
TACCCTTCCA ACGAAGACCT TCACGAAAGG CTGGAGGTTT TCAAGGCCCT CACAGACAAT 
GGGAGACACA TCACCTACTT GGAGGAAGAG CTGGCTGACT TTGTCCTGCA GTGGATGGAT 
GTTGGCTTGT CCTCGGAATT CCTTCTGGTG CTGGTGAACT TGGTCAAATT CAATAGCTGT 
TACCTCGACG AGTACATCGC AAGGATGGTT CAGATGATCT GTCTGCTGTG CGTCCGGACC 
GCGTCCTCTG TGGACATAGA GGTCTCCCTG CAGGTGCTGG ACGCCGTGGT CTGCTACAAC 
TGCCTGCCGG CTGAGAGCCT CCCGCTGTTC ATCGTTACCC TCTGTCGCAC CATCAACGTC 
AAGGAGCTCT GCGAGCCTTG CTGGAAGCTG ATGCGGAACC TCCTTGGCAC CCACCTGGGC 
CACAGCGCCA TCTACAACAT GTGCCACCTC ATGGAGGACA GAGCCTACAT GGAGGACGCG 
CCCCTGCTGA GAGGAGCCGT GTTTTTTGTG GGCATGGCTC TCTGGGGAGC CCACCGGCTC 
TATTCTCTCA GGAACTCGCC GACATCTGTG TTGCCATCAT TTTACCAGGC CATGGCATGT 
CCGAACGAGG TGGTGTCCTA TGAGATCGTC CTGTCCATCA CCAGGCTCAT CAAGAAGTAT 
AGGAAGGAGC TCCAGGTGGT GGCGTGGGAC ATTCTGCTGA ACATCATCGA ACGGCTCCTT 
CAGCAGCTCC AGACCTTGGA CAGCCCGGAG CTCAGGACCA TCGTCCATGA CCTGTTGACC 
ACGGTGGAGG AGCTGTGTGA CCAGAACGAG TTCCACGGGT CTCAGGAGAG ATACTTTGAA 
CTGGTGGAGA GATGTGCGGA CCAGAGGCCT GAGTCCTCCC TCCTGAACCT GATCTCCTAT 
AGAGCGCAGT CCATCCACCC GGCCAAGGAC GGCTGGATTC AGAACCTGCA GGCGCTGATG 
GAGAGATTCT TCAGGAGCGA GTCCCGAGGC GCCGTGCGCA TCAAGGTGCT GGACGTGCTG 
TCCTTTGTGC TGCTCATCAA CAGGCAGTTC TATGAGGAGG AGCTGATTAA CTCAGTGGTC 
ATCTCGCAGC TCTCCCACAT CCCCGAGGAT AAAGACCACC AGGTCCGAAA GCTGGCCACC 
CAGTTGCTGG TGGACCTGGC AGAGGGCTGC CACACACACC ACTTCAACAG CCTGCTGGAC 
ATCATCGAGA AGGTGATGGC CCGCTCCCTC TCCCCACCCC CGGAGCTGGA AGAAAGGGAT 
GTGGCCGCAT ACTCGGCCTC CTTGGAGGAT GTGAAGACAG CCGTCCTGGG GCTTCTGGTC 
ATCCTTCAGA CCAAGCTGTA CACCCTGCCT GCAAGCCACG CCACGCGTGT GTATGAGATG 
CTGGTCAGCC ACATTCAGCT CCACTACAAG CACAGCTACA CCCTGCCAAT CGCGAGCAGC 
ATCCGGCTGC AGGCCTTTGA CTTCCTGTTG CTGCTGCGGG CCGACTCACT GCACCGCCTG 
GGCCTGCCCA ACAAGGATGG AGTCGTGCGG TTCAGCCCCT ACTGCGTCTG CGACTACATG 
GAGCCAGAGA GAGGCTCTGA GAAGAAGACC AGCGGCCCCC TTTCTCCTCC CACAGGGCCT 
CCTGGCCCGG CGCCTGCAGG CCCCGCCGTG CGGCTGGGGT CCGTGCCCTA CTCCCTGCTC 
TTCCGCGTCC TGCTGCAGTG CTTGAAGCAG GAGTCTGACT GGAAGGTGCT GAAGCTGGTT 
CTGGGCAGGC TGCCTGAGTC CCTGCGCTAT AAAGTGCTCA TCTTTACTTC CCCTTGCAGT 
GTGGACCAGC TGTGCTCTGC TCTCTGCTCC ATGCTTTCAG GCCCAAAGAC ACTGGAGCGG 
CTCCGAGGCG CCCCAGAAGG CTTCTCCAGA ACTGACTTGC ACCTGGCCGT GGTTCCAGTG 
CTGACAGCAT TAATCTCTTA CCATAACTAC CTGGACAAAA CCAAACAGCG CGAGATGGTC 
TACTGCCTGG AGCAGGGCCT CATCCACCGC TGTGCCAGCC AGTGCGTCGT GGCCTTGTCC 
ATCTGCAGCG TGGAGATGCC TGACATCATC ATCAAGGCGC TGCCTGTTCT GGTGGTGAAG 
CTCACGCACA TCTCAGCCAC AGCCAGCATG GCCGTCCCAC TGCTGGAGTT CCTGTCCACT 
CTGGCCAGGC TGCCGCACCT CTACAGGAAC TTTGCCGCGG AGCAGTATGC CAGTGTGTTC 
GCCATCTCCC TGCCGTACAC CAACCCCTCC AAGTTTAATC AGTACATCGT GTGTCTGGCC 
CATCACGTCA TAGCCATGTG GTTCATCAGG TGCCGCCTGC CCTTCCGGAA GGATTTTGTC 
CCTTTCATCA CTAAGGGCCT GCGGTCCAAT GTCCTCTTGT CTTTTGATGA CACCCCCGAG 
AAGGACAGCT TCAGGGCCCG GAGTACTAGT CTCAACGAGA GACCCAAGAG GATACAGACG 
TCCCTCACCA GTGCCAGCTT GGGGTCTGCA GATGAGAACT CCGTGGCCCA GGCTGACGAT 
AGCCTGAAAA ACCTCCACCT GGAGCTCACG GAAACCTGTC TGGACATGAT GGCTCGATAC 
GTCTTCTCCA ACTTCACGGC TGTCCCGAAG AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG 
GGTGGCAGGA CCAAAACCTG GCTGGTTGGG AACAAGCTTG TCACTGTGAC GACAAGCGTG 
GGAACCGGGA CCCGGTCGTT ACTAGGCCTG GACTCGGGGG AGCTGCAGTC CGGCCCGGAG 
TCGAGCTCCA GCCCCGGGGT GCATGTGAGA CAGACCAAGG AGGCGCCGGC CAAGCTGGAG 
TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG GCCCGGGATC GGGTCCGTTC CATGTCGGGG 
GGCCATGGTC TTCGAGTTGG CGCCCTGGAC GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC 
ACTTCTCCAG GACCACGGAC TGCACCAGCC GCGAAACCTG AGAAGGCCTC AGCTGGCACC 
CGGGTTCCTG TGCAGGAGAA GACGAACCTG GCGGCCTATG TGCCCCTGCT GACCCAGGGC 
TGGGCGGAGA TCCTGGTCCG GAGGCCCACA GGGAACACCA GCTGGCTGAT GAGCCTGGAG 
AACCCGCTCA GCCCTTTCTC CTCGGACATC AACAACATGC CCCTGCAGGA GCTGTCTAAC 
GCCCTCATGG CGGCTGAGCG CTTCAAGGAG CACCGGGACA CAGCCCTGTA CAAGTCACTG 
TCGGTGCCGG CAGCCAGCAC GGCCAAACCC CCTCCTCTGC CTCGCTCCAA CACAGACTCC 
GCCGTGGTCA TGGAGGAGGG AAGTCCGGGC GAGGTTCCTG TGCTGGTGGA GCCCCCAGGG 
TTGGAGGACG TTGAGGCAGC GCTAGGCATG GACAGGCGCA CGGATGCCTA CAGCAGGTCG 
TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG CTCCACGCGG AGGAGCTGGT TGGCAGGGGC 
ATCCCCATCG AGCGAGTCGT CTCCTCGGAG GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC 
CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC 
ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC 
CGGTCACAGT CAGGGACCCT GGACGGGGAA AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC 
AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC 
CGGCCCCGAG GTTACACCAT CTCCGACTCG GCCCCATCAC GCAGGGGCAA GAGAGTAGAG 
AGGGACGCCT TAAAGAGCAG AGCCACAGCC TCCAATGCAG AGAAAGTGCC AGGCATCAAC 
CCCAGTTTCG TGTTCCTGCA GCTCTACCAT TCCCCCTTCT TTGGCGACGA GTCAAACAAG 
CCAATCCTGC TGCCCAATGA GTCACAGTCC TTTGAGCGGT CGGTGCAGCT CCTCGACCAG 
ATCCCATCAT ACGACACCCA CAAGATCGCC GTCCTGTATG TTGGAGAAGG CCAGAGCAAC 
AGCGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCCTACA GGTACACGGA GTTCCTGACG 
GGCCTGGGCC GGCTCATCGA GCTGAAGGAC TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC 
CTGGACGTGT GTGGTGAGGA CGGCCAGTTC ACCTACTGCT GGCACGATGA CATCATGCAA 
GCCGTCTTCC ACATCGCCAC CCTGATGCCC ACCAAGGACG TGGACAAGCA CCGCTGCGAC 
AAGAAGCGCC ACCTGGGCAA CGACTTTGTG TCCATTGTCT ACAATGACTC CGGTGAGGAC 
TTCAAGCTTG GCACCATCAA GGGCCAGTTC AACTTTGTCC ACGTGATCGT CACCCCGCTG 
GACTACGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAGGG CCTTGTGGAC 
ACCAGCGTGG CCAAGATCGT GTCTGACCGC AACCTGCCCT TCGTGGCCCG CCAGATGGCC 
CTGCACGCAA ATATGGCCTC ACAGGTGCAT CATAGCCGCT CCAACCCCAC CGATATCTAC 
CCCTCCAAGT GGATTGCCCG GCTCCGCCAC ATCAAGCGGC TCCGCCAGCG GATCTGCGAG 
GAAGCCGCCT ACTCCAACCC CAGCCTACCT CTGGTGCACC CTCCGTCCCA TAGCAAAGCC 
CCTGCACAGA CTCCAGCCGA GCCCACACCT GGCTATGAGG TGGGCCAGCG GAAGCGCCTC 
ATCTCCTCGG TGGAGGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001318831.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305760.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 8, mRNA.

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CloneID	OHu101969
Clone ID Related Accession (Same CDS sequence)	NM_001318831.1
Accession Version	NM_001318831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402106.1, BX647816.1, AK295672.1, AK299343.1 and CA427637.1. Transcript Variant: This variant (8) differs in the 5' UTR, uses a downstream start codon and contains multiple coding region differences, compared to variant 1. It encodes isoform 8, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCGGGCGAGG TTCCTGTGCT GGTGGAGCCC CCAGGGTTGG AGGACGTTGA GGCAGCGCTA 
GGCATGGACA GGCGCACGGA TGCCTACAGC AGGTCGTCCT CAGTCTCCAG CCAGGAGGAG 
AAGTCGCTCC ACGCGGAGGA GCTGGTTGGC AGGGGCATCC CCATCGAGCG AGTCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCGCAGCC CCTGAGCAAG 
TCCAGCTCCT CTCCCGAGCT GCAGACTCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCCGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGGT CACAGTCAGG GACCCTGGAC 
GGGGAAAGTG CTGCCTGGTC GGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCGCCCAGT GGCCTCCGGC CCCGAGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAAGAGA GTAGAGAGGG ACGCCTTAAA GAGCAGAGCC 
ACAGCCTCCA ATGCAGAGAA AGTGCCAGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC 
TACCATTCCC CCTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAT 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG 
AAGGACTGCC AGCCGGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGCGACAAGA AGCGCCACCT GGGCAACGAC 
TTTGTGTCCA TTGTCTACAA TGACTCCGGT GAGGACTTCA AGCTTGGCAC CATCAAGGGC 
CAGTTCAACT TTGTCCACGT GATCGTCACC CCGCTGGACT ACGAGTGCAA CCTGGTGTCC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA GATCGTGTCT 
GACCGCAACC TGCCCTTCGT GGCCCGCCAG ATGGCCCTGC ACGCAAATAT GGCCTCACAG 
GTGCATCATA GCCGCTCCAA CCCCACCGAT ATCTACCCCT CCAAGTGGAT TGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCAGCGGATC TGCGAGGAAG CCGCCTACTC CAACCCCAGC 
CTACCTCTGG TGCACCCTCC GTCCCATAGC AAAGCCCCTG CACAGACTCC AGCCGAGCCC 
ACACCTGGCT ATGAGGTGGG CCAGCGGAAG CGCCTCATCT CCTCGGTGGA GGACTTCACC 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305760.1
CDS	427..5118
Translation	MICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001318831.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318831.1

ORF Insert Sequence:

ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCGGGCGAGG TTCCTGTGCT GGTGGAGCCC CCAGGGTTGG AGGACGTTGA GGCAGCGCTA 
GGCATGGACA GGCGCACGGA TGCCTACAGC AGGTCGTCCT CAGTCTCCAG CCAGGAGGAG 
AAGTCGCTCC ACGCGGAGGA GCTGGTTGGC AGGGGCATCC CCATCGAGCG AGTCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCGCAGCC CCTGAGCAAG 
TCCAGCTCCT CTCCCGAGCT GCAGACTCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCCGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGGT CACAGTCAGG GACCCTGGAC 
GGGGAAAGTG CTGCCTGGTC GGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCGCCCAGT GGCCTCCGGC CCCGAGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAAGAGA GTAGAGAGGG ACGCCTTAAA GAGCAGAGCC 
ACAGCCTCCA ATGCAGAGAA AGTGCCAGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC 
TACCATTCCC CCTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAT 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG 
AAGGACTGCC AGCCGGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGCGACAAGA AGCGCCACCT GGGCAACGAC 
TTTGTGTCCA TTGTCTACAA TGACTCCGGT GAGGACTTCA AGCTTGGCAC CATCAAGGGC 
CAGTTCAACT TTGTCCACGT GATCGTCACC CCGCTGGACT ACGAGTGCAA CCTGGTGTCC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA GATCGTGTCT 
GACCGCAACC TGCCCTTCGT GGCCCGCCAG ATGGCCCTGC ACGCAAATAT GGCCTCACAG 
GTGCATCATA GCCGCTCCAA CCCCACCGAT ATCTACCCCT CCAAGTGGAT TGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCAGCGGATC TGCGAGGAAG CCGCCTACTC CAACCCCAGC 
CTACCTCTGG TGCACCCTCC GTCCCATAGC AAAGCCCCTG CACAGACTCC AGCCGAGCCC 
ACACCTGGCT ATGAGGTGGG CCAGCGGAAG CGCCTCATCT CCTCGGTGGA GGACTTCACC 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001370405.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357334.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 12, mRNA.

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CloneID	OHu108136
Clone ID Related Accession (Same CDS sequence)	NM_001370405.1
Accession Version	NM_001370405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093513.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.84449.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2153733 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TGTGCATCAT 
AGCCGCTCCA ACCCCACCGA TATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGCGGCTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTACCTCTG 
GTGCACCCTC CGTCCCATAG CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC 
TATGAGGTGG GCCAGCGGAA GCGCCTCATC TCCTCGGTGG AGGACTTCAC CGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357334.1
CDS	111..5393
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001370405.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370405.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TGTGCATCAT 
AGCCGCTCCA ACCCCACCGA TATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGCGGCTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTACCTCTG 
GTGCACCCTC CGTCCCATAG CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC 
TATGAGGTGG GCCAGCGGAA GCGCCTCATC TCCTCGGTGG AGGACTTCAC CGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001370404.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357333.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 11, mRNA.

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CloneID	OHu86241
Clone ID Related Accession (Same CDS sequence)	XM_017023616.1 , NM_001370404.1
Accession Version	NM_001370404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093513.3. On May 7, 2019 this sequence version replaced XM_017023616.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.154730.1, SRR1803613.262335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCGGGCGAGG TTCCTGTGCT GGTGGAGCCC CCAGGGTTGG AGGACGTTGA GGCAGCGCTA 
GGCATGGACA GGCGCACGGA TGCCTACAGC AGGTCGTCCT CAGTCTCCAG CCAGGAGGAG 
AAGTCGCTCC ACGCGGAGGA GCTGGTTGGC AGGGGCATCC CCATCGAGCG AGTCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCGCAGCC CCTGAGCAAG 
TCCAGCTCCT CTCCCGAGCT GCAGACTCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCCGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGGT CACAGTCAGG GACCCTGGAC 
GGGGAAAGTG CTGCCTGGTC GGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCGCCCAGT GGCCTCCGGC CCCGAGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAAGAGA GTAGAGAGGG ACGCCTTAAA GAGCAGAGCC 
ACAGCCTCCA ATGCAGAGAA AGTGCCAGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC 
TACCATTCCC CCTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAT 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG 
AAGGACTGCC AGCCGGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGCGACAAGA AGCGCCACCT GGGCAACGAC 
TTTGTGTCCA TTGTCTACAA TGACTCCGGT GAGGACTTCA AGCTTGGCAC CATCAAGGGC 
CAGTTCAACT TTGTCCACGT GATCGTCACC CCGCTGGACT ACGAGTGCAA CCTGGTGTCC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA GATCGTGTCT 
GACCGCAACC TGCCCTTCGT GGCCCGCCAG ATGGCCCTGC ACGCAAATAT GGCCTCACAG 
GTGCATCATA GCCGCTCCAA CCCCACCGAT ATCTACCCCT CCAAGTGGAT TGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCAGCGGATC TGCGAGGAAG CCGCCTACTC CAACCCCAGC 
CTACCTCTGG TGCACCCTCC GTCCCATAGC AAAGCCCCTG CACAGACTCC AGCCGAGCCC 
ACACCTGGCT ATGAGGTGGG CCAGCGGAAG CGCCTCATCT CCTCGGTGGA GGACTTCACC 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357333.1
CDS	111..5402
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001370404.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370404.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCGGGCGAGG TTCCTGTGCT GGTGGAGCCC CCAGGGTTGG AGGACGTTGA GGCAGCGCTA 
GGCATGGACA GGCGCACGGA TGCCTACAGC AGGTCGTCCT CAGTCTCCAG CCAGGAGGAG 
AAGTCGCTCC ACGCGGAGGA GCTGGTTGGC AGGGGCATCC CCATCGAGCG AGTCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCGCAGCC CCTGAGCAAG 
TCCAGCTCCT CTCCCGAGCT GCAGACTCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCCGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGGT CACAGTCAGG GACCCTGGAC 
GGGGAAAGTG CTGCCTGGTC GGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCGCCCAGT GGCCTCCGGC CCCGAGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAAGAGA GTAGAGAGGG ACGCCTTAAA GAGCAGAGCC 
ACAGCCTCCA ATGCAGAGAA AGTGCCAGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC 
TACCATTCCC CCTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAT 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG 
AAGGACTGCC AGCCGGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGCGACAAGA AGCGCCACCT GGGCAACGAC 
TTTGTGTCCA TTGTCTACAA TGACTCCGGT GAGGACTTCA AGCTTGGCAC CATCAAGGGC 
CAGTTCAACT TTGTCCACGT GATCGTCACC CCGCTGGACT ACGAGTGCAA CCTGGTGTCC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA GATCGTGTCT 
GACCGCAACC TGCCCTTCGT GGCCCGCCAG ATGGCCCTGC ACGCAAATAT GGCCTCACAG 
GTGCATCATA GCCGCTCCAA CCCCACCGAT ATCTACCCCT CCAAGTGGAT TGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCAGCGGATC TGCGAGGAAG CCGCCTACTC CAACCCCAGC 
CTACCTCTGG TGCACCCTCC GTCCCATAGC AAAGCCCCTG CACAGACTCC AGCCGAGCCC 
ACACCTGGCT ATGAGGTGGG CCAGCGGAAG CGCCTCATCT CCTCGGTGGA GGACTTCACC 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024450413.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306181.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.

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CloneID	OHu104194
Clone ID Related Accession (Same CDS sequence)	XM_024450413.1
Accession Version	XM_024450413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCGGGCGAG 
GTTCCTGTGC TGGTGGAGCC CCCAGGGTTG GAGGACGTTG AGGCAGCGCT AGGCATGGAC 
AGGCGCACGG ATGCCTACAG CAGGTCGTCC TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC 
CACGCGGAGG AGCTGGTTGG CAGGGGCATC CCCATCGAGC GAGTCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC 
TCTCCCGAGC TGCAGACTCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCCGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG GGACCCTGGA CGGGGAAAGT 
GCTGCCTGGT CGGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAAGAG AGTAGAGAGG GACGCCTTAA AGAGCAGAGC CACAGCCTCC 
AATGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCC 
CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT GAAGGACTGC 
CAGCCGGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCACCG CTGCGACAAG AAGCGCCACC TGGGCAACGA CTTTGTGTCC 
ATTGTCTACA ATGACTCCGG TGAGGACTTC AAGCTTGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCGTCAC CCCGCTGGAC TACGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATG TGAGTGGGGG TGGGTCCAGG 
CGTGAGCTGG TGGGACAGGC CCAGGTGCCA CCTGATAGTG AGCTCACCCC CTGCCTACGT 
CCCCAGATGG CCTCACAGGT GCATCATAGC CGCTCCAACC CCACCGATAT CTACCCCTCC 
AAGTGGATTG CCCGGCTCCG CCACATCAAG CGGCTCCGCC AGCGGATCTG CGAGGAAGCC 
GCCTACTCCA ACCCCAGCCT ACCTCTGGTG CACCCTCCGT CCCATAGCAA AGCCCCTGCA 
CAGACTCCAG CCGAGCCCAC ACCTGGCTAT GAGGTGGGCC AGCGGAAGCG CCTCATCTCC 
TCGGTGGAGG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306181.1
CDS	88..5397
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANVSGGGSRRELVGQAQVPPDSELTPCLRPQMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_024450413.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450413.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCGGGCGAG 
GTTCCTGTGC TGGTGGAGCC CCCAGGGTTG GAGGACGTTG AGGCAGCGCT AGGCATGGAC 
AGGCGCACGG ATGCCTACAG CAGGTCGTCC TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC 
CACGCGGAGG AGCTGGTTGG CAGGGGCATC CCCATCGAGC GAGTCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC 
TCTCCCGAGC TGCAGACTCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCCGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG GGACCCTGGA CGGGGAAAGT 
GCTGCCTGGT CGGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAAGAG AGTAGAGAGG GACGCCTTAA AGAGCAGAGC CACAGCCTCC 
AATGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCC 
CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT GAAGGACTGC 
CAGCCGGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCACCG CTGCGACAAG AAGCGCCACC TGGGCAACGA CTTTGTGTCC 
ATTGTCTACA ATGACTCCGG TGAGGACTTC AAGCTTGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCGTCAC CCCGCTGGAC TACGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATG TGAGTGGGGG TGGGTCCAGG 
CGTGAGCTGG TGGGACAGGC CCAGGTGCCA CCTGATAGTG AGCTCACCCC CTGCCTACGT 
CCCCAGATGG CCTCACAGGT GCATCATAGC CGCTCCAACC CCACCGATAT CTACCCCTCC 
AAGTGGATTG CCCGGCTCCG CCACATCAAG CGGCTCCGCC AGCGGATCTG CGAGGAAGCC 
GCCTACTCCA ACCCCAGCCT ACCTCTGGTG CACCCTCCGT CCCATAGCAA AGCCCCTGCA 
CAGACTCCAG CCGAGCCCAC ACCTGGCTAT GAGGTGGGCC AGCGGAAGCG CCTCATCTCC 
TCGGTGGAGG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_017023615.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879104.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

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CloneID	OHu86239
Clone ID Related Accession (Same CDS sequence)	XM_017023615.1
Accession Version	XM_017023615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATA CAGACGTCCC TCACCAGTGC CAGCTTGGGG 
TCTGCAGATG AGAACTCCGT GGCCCAGGCT GACGATAGCC TGAAAAACCT CCACCTGGAG 
CTCACGGAAA CCTGTCTGGA CATGATGGCT CGATACGTCT TCTCCAACTT CACGGCTGTC 
CCGAAGAGGT CTCCTGTGGG CGAGTTCCTC CTAGCGGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC TGTGACGACA AGCGTGGGAA CCGGGACCCG GTCGTTACTA 
GGCCTGGACT CGGGGGAGCT GCAGTCCGGC CCGGAGTCGA GCTCCAGCCC CGGGGTGCAT 
GTGAGACAGA CCAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGATCGGGT CCGTTCCATG TCGGGGGGCC ATGGTCTTCG AGTTGGCGCC 
CTGGACGTGC CGGCCTCCCA GTTCCTGGGC AGTGCCACTT CTCCAGGACC ACGGACTGCA 
CCAGCCGCGA AACCTGAGAA GGCCTCAGCT GGCACCCGGG TTCCTGTGCA GGAGAAGACG 
AACCTGGCGG CCTATGTGCC CCTGCTGACC CAGGGCTGGG CGGAGATCCT GGTCCGGAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC TTTCTCCTCG 
GACATCAACA ACATGCCCCT GCAGGAGCTG TCTAACGCCC TCATGGCGGC TGAGCGCTTC 
AAGGAGCACC GGGACACAGC CCTGTACAAG TCACTGTCGG TGCCGGCAGC CAGCACGGCC 
AAACCCCCTC CTCTGCCTCG CTCCAACACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCCGCCGT GGTCATGGAG 
GAGGGAAGTC CGGGCGAGGT TCCTGTGCTG GTGGAGCCCC CAGGGTTGGA GGACGTTGAG 
GCAGCGCTAG GCATGGACAG GCGCACGGAT GCCTACAGCA GGTCGTCCTC AGTCTCCAGC 
CAGGAGGAGA AGTCGCTCCA CGCGGAGGAG CTGGTTGGCA GGGGCATCCC CATCGAGCGA 
GTCGTCTCCT CGGAGGGTGG CCGGCCCTCT GTGGACCTCT CCTTCCAGCC CTCGCAGCCC 
CTGAGCAAGT CCAGCTCCTC TCCCGAGCTG CAGACTCTGC AGGACATCCT CGGGGACCCT 
GGGGACAAGG CCGACGTGGG CCGGCTGAGC CCTGAGGTTA AGGCCCGGTC ACAGTCAGGG 
ACCCTGGACG GGGAAAGTGC TGCCTGGTCG GCCTCGGGCG AAGACAGTCG GGGCCAGCCC 
GAGGGTCCCT TGCCTTCCAG CTCCCCCCGC TCGCCCAGTG GCCTCCGGCC CCGAGGTTAC 
ACCATCTCCG ACTCGGCCCC ATCACGCAGG GGCAAGAGAG TAGAGAGGGA CGCCTTAAAG 
AGCAGAGCCA CAGCCTCCAA TGCAGAGAAA GTGCCAGGCA TCAACCCCAG TTTCGTGTTC 
CTGCAGCTCT ACCATTCCCC CTTCTTTGGC GACGAGTCAA ACAAGCCAAT CCTGCTGCCC 
AATGAGTCAC AGTCCTTTGA GCGGTCGGTG CAGCTCCTCG ACCAGATCCC ATCATACGAC 
ACCCACAAGA TCGCCGTCCT GTATGTTGGA GAAGGCCAGA GCAACAGCGA GCTCGCCATC 
CTGTCCAATG AGCATGGCTC CTACAGGTAC ACGGAGTTCC TGACGGGCCT GGGCCGGCTC 
ATCGAGCTGA AGGACTGCCA GCCGGACAAG GTGTACCTGG GAGGCCTGGA CGTGTGTGGT 
GAGGACGGCC AGTTCACCTA CTGCTGGCAC GATGACATCA TGCAAGCCGT CTTCCACATC 
GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GCGACAAGAA GCGCCACCTG 
GGCAACGACT TTGTGTCCAT TGTCTACAAT GACTCCGGTG AGGACTTCAA GCTTGGCACC 
ATCAAGGGCC AGTTCAACTT TGTCCACGTG ATCGTCACCC CGCTGGACTA CGAGTGCAAC 
CTGGTGTCCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG CGTGGCCAAG 
ATCGTGTCTG ACCGCAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA CGCAAATATG 
GCCTCACAGG TGCATCATAG CCGCTCCAAC CCCACCGATA TCTACCCCTC CAAGTGGATT 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CAGCGGATCT GCGAGGAAGC CGCCTACTCC 
AACCCCAGCC TACCTCTGGT GCACCCTCCG TCCCATAGCA AAGCCCCTGC ACAGACTCCA 
GCCGAGCCCA CACCTGGCTA TGAGGTGGGC CAGCGGAAGC GCCTCATCTC CTCGGTGGAG 
GACTTCACCG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879104.1
CDS	88..5508
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_017023615.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023615.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATA CAGACGTCCC TCACCAGTGC CAGCTTGGGG 
TCTGCAGATG AGAACTCCGT GGCCCAGGCT GACGATAGCC TGAAAAACCT CCACCTGGAG 
CTCACGGAAA CCTGTCTGGA CATGATGGCT CGATACGTCT TCTCCAACTT CACGGCTGTC 
CCGAAGAGGT CTCCTGTGGG CGAGTTCCTC CTAGCGGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC TGTGACGACA AGCGTGGGAA CCGGGACCCG GTCGTTACTA 
GGCCTGGACT CGGGGGAGCT GCAGTCCGGC CCGGAGTCGA GCTCCAGCCC CGGGGTGCAT 
GTGAGACAGA CCAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGATCGGGT CCGTTCCATG TCGGGGGGCC ATGGTCTTCG AGTTGGCGCC 
CTGGACGTGC CGGCCTCCCA GTTCCTGGGC AGTGCCACTT CTCCAGGACC ACGGACTGCA 
CCAGCCGCGA AACCTGAGAA GGCCTCAGCT GGCACCCGGG TTCCTGTGCA GGAGAAGACG 
AACCTGGCGG CCTATGTGCC CCTGCTGACC CAGGGCTGGG CGGAGATCCT GGTCCGGAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC TTTCTCCTCG 
GACATCAACA ACATGCCCCT GCAGGAGCTG TCTAACGCCC TCATGGCGGC TGAGCGCTTC 
AAGGAGCACC GGGACACAGC CCTGTACAAG TCACTGTCGG TGCCGGCAGC CAGCACGGCC 
AAACCCCCTC CTCTGCCTCG CTCCAACACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCCGCCGT GGTCATGGAG 
GAGGGAAGTC CGGGCGAGGT TCCTGTGCTG GTGGAGCCCC CAGGGTTGGA GGACGTTGAG 
GCAGCGCTAG GCATGGACAG GCGCACGGAT GCCTACAGCA GGTCGTCCTC AGTCTCCAGC 
CAGGAGGAGA AGTCGCTCCA CGCGGAGGAG CTGGTTGGCA GGGGCATCCC CATCGAGCGA 
GTCGTCTCCT CGGAGGGTGG CCGGCCCTCT GTGGACCTCT CCTTCCAGCC CTCGCAGCCC 
CTGAGCAAGT CCAGCTCCTC TCCCGAGCTG CAGACTCTGC AGGACATCCT CGGGGACCCT 
GGGGACAAGG CCGACGTGGG CCGGCTGAGC CCTGAGGTTA AGGCCCGGTC ACAGTCAGGG 
ACCCTGGACG GGGAAAGTGC TGCCTGGTCG GCCTCGGGCG AAGACAGTCG GGGCCAGCCC 
GAGGGTCCCT TGCCTTCCAG CTCCCCCCGC TCGCCCAGTG GCCTCCGGCC CCGAGGTTAC 
ACCATCTCCG ACTCGGCCCC ATCACGCAGG GGCAAGAGAG TAGAGAGGGA CGCCTTAAAG 
AGCAGAGCCA CAGCCTCCAA TGCAGAGAAA GTGCCAGGCA TCAACCCCAG TTTCGTGTTC 
CTGCAGCTCT ACCATTCCCC CTTCTTTGGC GACGAGTCAA ACAAGCCAAT CCTGCTGCCC 
AATGAGTCAC AGTCCTTTGA GCGGTCGGTG CAGCTCCTCG ACCAGATCCC ATCATACGAC 
ACCCACAAGA TCGCCGTCCT GTATGTTGGA GAAGGCCAGA GCAACAGCGA GCTCGCCATC 
CTGTCCAATG AGCATGGCTC CTACAGGTAC ACGGAGTTCC TGACGGGCCT GGGCCGGCTC 
ATCGAGCTGA AGGACTGCCA GCCGGACAAG GTGTACCTGG GAGGCCTGGA CGTGTGTGGT 
GAGGACGGCC AGTTCACCTA CTGCTGGCAC GATGACATCA TGCAAGCCGT CTTCCACATC 
GCCACCCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GCGACAAGAA GCGCCACCTG 
GGCAACGACT TTGTGTCCAT TGTCTACAAT GACTCCGGTG AGGACTTCAA GCTTGGCACC 
ATCAAGGGCC AGTTCAACTT TGTCCACGTG ATCGTCACCC CGCTGGACTA CGAGTGCAAC 
CTGGTGTCCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG CGTGGCCAAG 
ATCGTGTCTG ACCGCAACCT GCCCTTCGTG GCCCGCCAGA TGGCCCTGCA CGCAAATATG 
GCCTCACAGG TGCATCATAG CCGCTCCAAC CCCACCGATA TCTACCCCTC CAAGTGGATT 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CAGCGGATCT GCGAGGAAGC CGCCTACTCC 
AACCCCAGCC TACCTCTGGT GCACCCTCCG TCCCATAGCA AAGCCCCTGC ACAGACTCCA 
GCCGAGCCCA CACCTGGCTA TGAGGTGGGC CAGCGGAAGC GCCTCATCTC CTCGGTGGAG 
GACTTCACCG AGTTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_017023616.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879105.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

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CloneID	OHu86241
Clone ID Related Accession (Same CDS sequence)	XM_017023616.1 , NM_001370404.1
Accession Version	XM_017023616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCGGGCGAGG TTCCTGTGCT GGTGGAGCCC CCAGGGTTGG AGGACGTTGA GGCAGCGCTA 
GGCATGGACA GGCGCACGGA TGCCTACAGC AGGTCGTCCT CAGTCTCCAG CCAGGAGGAG 
AAGTCGCTCC ACGCGGAGGA GCTGGTTGGC AGGGGCATCC CCATCGAGCG AGTCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCGCAGCC CCTGAGCAAG 
TCCAGCTCCT CTCCCGAGCT GCAGACTCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCCGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGGT CACAGTCAGG GACCCTGGAC 
GGGGAAAGTG CTGCCTGGTC GGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCGCCCAGT GGCCTCCGGC CCCGAGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAAGAGA GTAGAGAGGG ACGCCTTAAA GAGCAGAGCC 
ACAGCCTCCA ATGCAGAGAA AGTGCCAGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC 
TACCATTCCC CCTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAT 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG 
AAGGACTGCC AGCCGGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGCGACAAGA AGCGCCACCT GGGCAACGAC 
TTTGTGTCCA TTGTCTACAA TGACTCCGGT GAGGACTTCA AGCTTGGCAC CATCAAGGGC 
CAGTTCAACT TTGTCCACGT GATCGTCACC CCGCTGGACT ACGAGTGCAA CCTGGTGTCC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA GATCGTGTCT 
GACCGCAACC TGCCCTTCGT GGCCCGCCAG ATGGCCCTGC ACGCAAATAT GGCCTCACAG 
GTGCATCATA GCCGCTCCAA CCCCACCGAT ATCTACCCCT CCAAGTGGAT TGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCAGCGGATC TGCGAGGAAG CCGCCTACTC CAACCCCAGC 
CTACCTCTGG TGCACCCTCC GTCCCATAGC AAAGCCCCTG CACAGACTCC AGCCGAGCCC 
ACACCTGGCT ATGAGGTGGG CCAGCGGAAG CGCCTCATCT CCTCGGTGGA GGACTTCACC 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879105.1
CDS	88..5379
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_017023616.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023616.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCCGCCG TGGTCATGGA GGAGGGAAGT 
CCGGGCGAGG TTCCTGTGCT GGTGGAGCCC CCAGGGTTGG AGGACGTTGA GGCAGCGCTA 
GGCATGGACA GGCGCACGGA TGCCTACAGC AGGTCGTCCT CAGTCTCCAG CCAGGAGGAG 
AAGTCGCTCC ACGCGGAGGA GCTGGTTGGC AGGGGCATCC CCATCGAGCG AGTCGTCTCC 
TCGGAGGGTG GCCGGCCCTC TGTGGACCTC TCCTTCCAGC CCTCGCAGCC CCTGAGCAAG 
TCCAGCTCCT CTCCCGAGCT GCAGACTCTG CAGGACATCC TCGGGGACCC TGGGGACAAG 
GCCGACGTGG GCCGGCTGAG CCCTGAGGTT AAGGCCCGGT CACAGTCAGG GACCCTGGAC 
GGGGAAAGTG CTGCCTGGTC GGCCTCGGGC GAAGACAGTC GGGGCCAGCC CGAGGGTCCC 
TTGCCTTCCA GCTCCCCCCG CTCGCCCAGT GGCCTCCGGC CCCGAGGTTA CACCATCTCC 
GACTCGGCCC CATCACGCAG GGGCAAGAGA GTAGAGAGGG ACGCCTTAAA GAGCAGAGCC 
ACAGCCTCCA ATGCAGAGAA AGTGCCAGGC ATCAACCCCA GTTTCGTGTT CCTGCAGCTC 
TACCATTCCC CCTTCTTTGG CGACGAGTCA AACAAGCCAA TCCTGCTGCC CAATGAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAT 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG 
AAGGACTGCC AGCCGGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGACGGC 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCAAGCCG TCTTCCACAT CGCCACCCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGCGACAAGA AGCGCCACCT GGGCAACGAC 
TTTGTGTCCA TTGTCTACAA TGACTCCGGT GAGGACTTCA AGCTTGGCAC CATCAAGGGC 
CAGTTCAACT TTGTCCACGT GATCGTCACC CCGCTGGACT ACGAGTGCAA CCTGGTGTCC 
CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GCGTGGCCAA GATCGTGTCT 
GACCGCAACC TGCCCTTCGT GGCCCGCCAG ATGGCCCTGC ACGCAAATAT GGCCTCACAG 
GTGCATCATA GCCGCTCCAA CCCCACCGAT ATCTACCCCT CCAAGTGGAT TGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCAGCGGATC TGCGAGGAAG CCGCCTACTC CAACCCCAGC 
CTACCTCTGG TGCACCCTCC GTCCCATAGC AAAGCCCCTG CACAGACTCC AGCCGAGCCC 
ACACCTGGCT ATGAGGTGGG CCAGCGGAAG CGCCTCATCT CCTCGGTGGA GGACTTCACC 
GAGTTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_000548.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000539.2 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 1, mRNA.

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CloneID	OHu28113
Clone ID Related Accession (Same CDS sequence)	NM_000548.4 , NM_000548.5
Accession Version	NM_000548.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	tuberin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402106.1, AK299343.1, X75621.1, AK295672.1 and CA427637.1. On Jan 14, 2016 this sequence version replaced NM_000548.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: X75621.1, LC224284.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG 
GAGGAGGGAA GTCCGGGCGA GGTTCCTGTG CTGGTGGAGC CCCCAGGGTT GGAGGACGTT 
GAGGCAGCGC TAGGCATGGA CAGGCGCACG GATGCCTACA GCAGGTCGTC CTCAGTCTCC 
AGCCAGGAGG AGAAGTCGCT CCACGCGGAG GAGCTGGTTG GCAGGGGCAT CCCCATCGAG 
CGAGTCGTCT CCTCGGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCGCAG 
CCCCTGAGCA AGTCCAGCTC CTCTCCCGAG CTGCAGACTC TGCAGGACAT CCTCGGGGAC 
CCTGGGGACA AGGCCGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG GTCACAGTCA 
GGGACCCTGG ACGGGGAAAG TGCTGCCTGG TCGGCCTCGG GCGAAGACAG TCGGGGCCAG 
CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCGCCCA GTGGCCTCCG GCCCCGAGGT 
TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAAGA GAGTAGAGAG GGACGCCTTA 
AAGAGCAGAG CCACAGCCTC CAATGCAGAG AAAGTGCCAG GCATCAACCC CAGTTTCGTG 
TTCCTGCAGC TCTACCATTC CCCCTTCTTT GGCGACGAGT CAAACAAGCC AATCCTGCTG 
CCCAATGAGT CACAGTCCTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCATCATAC 
GACACCCACA AGATCGCCGT CCTGTATGTT GGAGAAGGCC AGAGCAACAG CGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCCTACAGG TACACGGAGT TCCTGACGGG CCTGGGCCGG 
CTCATCGAGC TGAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGAGGCCT GGACGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGCGACAA GAAGCGCCAC 
CTGGGCAACG ACTTTGTGTC CATTGTCTAC AATGACTCCG GTGAGGACTT CAAGCTTGGC 
ACCATCAAGG GCCAGTTCAA CTTTGTCCAC GTGATCGTCA CCCCGCTGGA CTACGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC 
AAGATCGTGT CTGACCGCAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCACGCAAAT 
ATGGCCTCAC AGGTGCATCA TAGCCGCTCC AACCCCACCG ATATCTACCC CTCCAAGTGG 
ATTGCCCGGC TCCGCCACAT CAAGCGGCTC CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC 
TCCAACCCCA GCCTACCTCT GGTGCACCCT CCGTCCCATA GCAAAGCCCC TGCACAGACT 
CCAGCCGAGC CCACACCTGG CTATGAGGTG GGCCAGCGGA AGCGCCTCAT CTCCTCGGTG 
GAGGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000539.2
CDS	201..5624
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_000548.4
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000548.4

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG 
GAGGAGGGAA GTCCGGGCGA GGTTCCTGTG CTGGTGGAGC CCCCAGGGTT GGAGGACGTT 
GAGGCAGCGC TAGGCATGGA CAGGCGCACG GATGCCTACA GCAGGTCGTC CTCAGTCTCC 
AGCCAGGAGG AGAAGTCGCT CCACGCGGAG GAGCTGGTTG GCAGGGGCAT CCCCATCGAG 
CGAGTCGTCT CCTCGGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCGCAG 
CCCCTGAGCA AGTCCAGCTC CTCTCCCGAG CTGCAGACTC TGCAGGACAT CCTCGGGGAC 
CCTGGGGACA AGGCCGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG GTCACAGTCA 
GGGACCCTGG ACGGGGAAAG TGCTGCCTGG TCGGCCTCGG GCGAAGACAG TCGGGGCCAG 
CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCGCCCA GTGGCCTCCG GCCCCGAGGT 
TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAAGA GAGTAGAGAG GGACGCCTTA 
AAGAGCAGAG CCACAGCCTC CAATGCAGAG AAAGTGCCAG GCATCAACCC CAGTTTCGTG 
TTCCTGCAGC TCTACCATTC CCCCTTCTTT GGCGACGAGT CAAACAAGCC AATCCTGCTG 
CCCAATGAGT CACAGTCCTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCATCATAC 
GACACCCACA AGATCGCCGT CCTGTATGTT GGAGAAGGCC AGAGCAACAG CGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCCTACAGG TACACGGAGT TCCTGACGGG CCTGGGCCGG 
CTCATCGAGC TGAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGAGGCCT GGACGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGCGACAA GAAGCGCCAC 
CTGGGCAACG ACTTTGTGTC CATTGTCTAC AATGACTCCG GTGAGGACTT CAAGCTTGGC 
ACCATCAAGG GCCAGTTCAA CTTTGTCCAC GTGATCGTCA CCCCGCTGGA CTACGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC 
AAGATCGTGT CTGACCGCAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCACGCAAAT 
ATGGCCTCAC AGGTGCATCA TAGCCGCTCC AACCCCACCG ATATCTACCC CTCCAAGTGG 
ATTGCCCGGC TCCGCCACAT CAAGCGGCTC CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC 
TCCAACCCCA GCCTACCTCT GGTGCACCCT CCGTCCCATA GCAAAGCCCC TGCACAGACT 
CCAGCCGAGC CCACACCTGG CTATGAGGTG GGCCAGCGGA AGCGCCTCAT CTCCTCGGTG 
GAGGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_000548.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000539.2 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 1, mRNA.

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CloneID	OHu28113
Clone ID Related Accession (Same CDS sequence)	NM_000548.4 , NM_000548.5
Accession Version	NM_000548.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402106.1, AK299343.1, X75621.1, AK295672.1, CA427637.1 and AC093513.3. On Nov 23, 2018 this sequence version replaced NM_000548.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: X75621.1, LC224284.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000219476.9/ ENSP00000219476.3 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG 
GAGGAGGGAA GTCCGGGCGA GGTTCCTGTG CTGGTGGAGC CCCCAGGGTT GGAGGACGTT 
GAGGCAGCGC TAGGCATGGA CAGGCGCACG GATGCCTACA GCAGGTCGTC CTCAGTCTCC 
AGCCAGGAGG AGAAGTCGCT CCACGCGGAG GAGCTGGTTG GCAGGGGCAT CCCCATCGAG 
CGAGTCGTCT CCTCGGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCGCAG 
CCCCTGAGCA AGTCCAGCTC CTCTCCCGAG CTGCAGACTC TGCAGGACAT CCTCGGGGAC 
CCTGGGGACA AGGCCGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG GTCACAGTCA 
GGGACCCTGG ACGGGGAAAG TGCTGCCTGG TCGGCCTCGG GCGAAGACAG TCGGGGCCAG 
CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCGCCCA GTGGCCTCCG GCCCCGAGGT 
TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAAGA GAGTAGAGAG GGACGCCTTA 
AAGAGCAGAG CCACAGCCTC CAATGCAGAG AAAGTGCCAG GCATCAACCC CAGTTTCGTG 
TTCCTGCAGC TCTACCATTC CCCCTTCTTT GGCGACGAGT CAAACAAGCC AATCCTGCTG 
CCCAATGAGT CACAGTCCTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCATCATAC 
GACACCCACA AGATCGCCGT CCTGTATGTT GGAGAAGGCC AGAGCAACAG CGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCCTACAGG TACACGGAGT TCCTGACGGG CCTGGGCCGG 
CTCATCGAGC TGAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGAGGCCT GGACGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGCGACAA GAAGCGCCAC 
CTGGGCAACG ACTTTGTGTC CATTGTCTAC AATGACTCCG GTGAGGACTT CAAGCTTGGC 
ACCATCAAGG GCCAGTTCAA CTTTGTCCAC GTGATCGTCA CCCCGCTGGA CTACGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC 
AAGATCGTGT CTGACCGCAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCACGCAAAT 
ATGGCCTCAC AGGTGCATCA TAGCCGCTCC AACCCCACCG ATATCTACCC CTCCAAGTGG 
ATTGCCCGGC TCCGCCACAT CAAGCGGCTC CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC 
TCCAACCCCA GCCTACCTCT GGTGCACCCT CCGTCCCATA GCAAAGCCCC TGCACAGACT 
CCAGCCGAGC CCACACCTGG CTATGAGGTG GGCCAGCGGA AGCGCCTCAT CTCCTCGGTG 
GAGGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000539.2
CDS	111..5534
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_000548.5
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000548.5

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCCGC CGTGGTCATG 
GAGGAGGGAA GTCCGGGCGA GGTTCCTGTG CTGGTGGAGC CCCCAGGGTT GGAGGACGTT 
GAGGCAGCGC TAGGCATGGA CAGGCGCACG GATGCCTACA GCAGGTCGTC CTCAGTCTCC 
AGCCAGGAGG AGAAGTCGCT CCACGCGGAG GAGCTGGTTG GCAGGGGCAT CCCCATCGAG 
CGAGTCGTCT CCTCGGAGGG TGGCCGGCCC TCTGTGGACC TCTCCTTCCA GCCCTCGCAG 
CCCCTGAGCA AGTCCAGCTC CTCTCCCGAG CTGCAGACTC TGCAGGACAT CCTCGGGGAC 
CCTGGGGACA AGGCCGACGT GGGCCGGCTG AGCCCTGAGG TTAAGGCCCG GTCACAGTCA 
GGGACCCTGG ACGGGGAAAG TGCTGCCTGG TCGGCCTCGG GCGAAGACAG TCGGGGCCAG 
CCCGAGGGTC CCTTGCCTTC CAGCTCCCCC CGCTCGCCCA GTGGCCTCCG GCCCCGAGGT 
TACACCATCT CCGACTCGGC CCCATCACGC AGGGGCAAGA GAGTAGAGAG GGACGCCTTA 
AAGAGCAGAG CCACAGCCTC CAATGCAGAG AAAGTGCCAG GCATCAACCC CAGTTTCGTG 
TTCCTGCAGC TCTACCATTC CCCCTTCTTT GGCGACGAGT CAAACAAGCC AATCCTGCTG 
CCCAATGAGT CACAGTCCTT TGAGCGGTCG GTGCAGCTCC TCGACCAGAT CCCATCATAC 
GACACCCACA AGATCGCCGT CCTGTATGTT GGAGAAGGCC AGAGCAACAG CGAGCTCGCC 
ATCCTGTCCA ATGAGCATGG CTCCTACAGG TACACGGAGT TCCTGACGGG CCTGGGCCGG 
CTCATCGAGC TGAAGGACTG CCAGCCGGAC AAGGTGTACC TGGGAGGCCT GGACGTGTGT 
GGTGAGGACG GCCAGTTCAC CTACTGCTGG CACGATGACA TCATGCAAGC CGTCTTCCAC 
ATCGCCACCC TGATGCCCAC CAAGGACGTG GACAAGCACC GCTGCGACAA GAAGCGCCAC 
CTGGGCAACG ACTTTGTGTC CATTGTCTAC AATGACTCCG GTGAGGACTT CAAGCTTGGC 
ACCATCAAGG GCCAGTTCAA CTTTGTCCAC GTGATCGTCA CCCCGCTGGA CTACGAGTGC 
AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAGGGCC TTGTGGACAC CAGCGTGGCC 
AAGATCGTGT CTGACCGCAA CCTGCCCTTC GTGGCCCGCC AGATGGCCCT GCACGCAAAT 
ATGGCCTCAC AGGTGCATCA TAGCCGCTCC AACCCCACCG ATATCTACCC CTCCAAGTGG 
ATTGCCCGGC TCCGCCACAT CAAGCGGCTC CGCCAGCGGA TCTGCGAGGA AGCCGCCTAC 
TCCAACCCCA GCCTACCTCT GGTGCACCCT CCGTCCCATA GCAAAGCCCC TGCACAGACT 
CCAGCCGAGC CCACACCTGG CTATGAGGTG GGCCAGCGGA AGCGCCTCAT CTCCTCGGTG 
GAGGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001114382.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107854.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 5, mRNA.

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CloneID	OHu28168
Clone ID Related Accession (Same CDS sequence)	NM_001114382.2
Accession Version	NM_001114382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402106.1, BC150300.1, AK295672.1 and CA427637.1. On Jan 14, 2016 this sequence version replaced NM_001114382.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 5, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107854.1
CDS	201..5555
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001114382.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114382.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001077183.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001070651.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 4, mRNA.

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CloneID	OHu28176
Clone ID Related Accession (Same CDS sequence)	NM_001077183.2
Accession Version	NM_001077183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402106.1, AB210000.1 and CA427637.1. On Jan 14, 2016 this sequence version replaced NM_001077183.1. Transcript Variant: This variant (4) differs in multiple places compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB210000.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCGGGCGAG 
GTTCCTGTGC TGGTGGAGCC CCCAGGGTTG GAGGACGTTG AGGCAGCGCT AGGCATGGAC 
AGGCGCACGG ATGCCTACAG CAGGTCGTCC TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC 
CACGCGGAGG AGCTGGTTGG CAGGGGCATC CCCATCGAGC GAGTCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC 
TCTCCCGAGC TGCAGACTCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCCGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG GGACCCTGGA CGGGGAAAGT 
GCTGCCTGGT CGGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAAGAG AGTAGAGAGG GACGCCTTAA AGAGCAGAGC CACAGCCTCC 
AATGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCC 
CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT GAAGGACTGC 
CAGCCGGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCACCG CTGCGACAAG AAGCGCCACC TGGGCAACGA CTTTGTGTCC 
ATTGTCTACA ATGACTCCGG TGAGGACTTC AAGCTTGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCGTCAC CCCGCTGGAC TACGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATA TGGCCTCACA GGTGCATCAT 
AGCCGCTCCA ACCCCACCGA TATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGCGGCTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTACCTCTG 
GTGCACCCTC CGTCCCATAG CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC 
TATGAGGTGG GCCAGCGGAA GCGCCTCATC TCCTCGGTGG AGGACTTCAC CGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070651.1
CDS	201..5423
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001077183.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077183.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGACTCCGCC GTGGTCATGG AGGAGGGAAG TCCGGGCGAG 
GTTCCTGTGC TGGTGGAGCC CCCAGGGTTG GAGGACGTTG AGGCAGCGCT AGGCATGGAC 
AGGCGCACGG ATGCCTACAG CAGGTCGTCC TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC 
CACGCGGAGG AGCTGGTTGG CAGGGGCATC CCCATCGAGC GAGTCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC 
TCTCCCGAGC TGCAGACTCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCCGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG GGACCCTGGA CGGGGAAAGT 
GCTGCCTGGT CGGCCTCGGG CGAAGACAGT CGGGGCCAGC CCGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT ACACCATCTC CGACTCGGCC 
CCATCACGCA GGGGCAAGAG AGTAGAGAGG GACGCCTTAA AGAGCAGAGC CACAGCCTCC 
AATGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCC 
CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA TCCTGTCCAA TGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT GAAGGACTGC 
CAGCCGGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG GTGAGGACGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA TCGCCACCCT GATGCCCACC 
AAGGACGTGG ACAAGCACCG CTGCGACAAG AAGCGCCACC TGGGCAACGA CTTTGTGTCC 
ATTGTCTACA ATGACTCCGG TGAGGACTTC AAGCTTGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCGTCAC CCCGCTGGAC TACGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATA TGGCCTCACA GGTGCATCAT 
AGCCGCTCCA ACCCCACCGA TATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGCGGCTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT CCAACCCCAG CCTACCTCTG 
GTGCACCCTC CGTCCCATAG CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC 
TATGAGGTGG GCCAGCGGAA GCGCCTCATC TCCTCGGTGG AGGACTTCAC CGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_021055.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_066399.2 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 2, mRNA.

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CloneID	OHu61157
Clone ID Related Accession (Same CDS sequence)	NM_021055.2 , XM_005255529.4
Accession Version	NM_021055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093513.3 and CA427637.1. On or before Jun 6, 2018 this sequence version replaced XM_005255529.4, NM_021055.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066399.2
CDS	111..5405
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_021055.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021055.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001363528.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350457.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 10, mRNA.

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CloneID	OHu61158
Clone ID Related Accession (Same CDS sequence)	NM_001363528.1 , XM_005255531.4
Accession Version	NM_001363528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093513.3 and KJ534954.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCGGGC 
GAGGTTCCTG TGCTGGTGGA GCCCCCAGGG TTGGAGGACG TTGAGGCAGC GCTAGGCATG 
GACAGGCGCA CGGATGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG 
CTCCACGCGG AGGAGCTGGT TGGCAGGGGC ATCCCCATCG AGCGAGTCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC 
TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA 
AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAA GAGAGTAGAG AGGGACGCCT TAAAGAGCAG AGCCACAGCC 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA CGACTTTGTG 
TCCATTGTCT ACAATGACTC CGGTGAGGAC TTCAAGCTTG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTACGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCAA ATATGGCCTC ACAGGTGCAT 
CATAGCCGCT CCAACCCCAC CGATATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGCGGC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTACCT 
CTGGTGCACC CTCCGTCCCA TAGCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT 
GGCTATGAGG TGGGCCAGCG GAAGCGCCTC ATCTCCTCGG TGGAGGACTT CACCGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350457.1
CDS	201..5426
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001363528.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363528.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCGGGC 
GAGGTTCCTG TGCTGGTGGA GCCCCCAGGG TTGGAGGACG TTGAGGCAGC GCTAGGCATG 
GACAGGCGCA CGGATGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG 
CTCCACGCGG AGGAGCTGGT TGGCAGGGGC ATCCCCATCG AGCGAGTCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC 
TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA 
AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAA GAGAGTAGAG AGGGACGCCT TAAAGAGCAG AGCCACAGCC 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA CGACTTTGTG 
TCCATTGTCT ACAATGACTC CGGTGAGGAC TTCAAGCTTG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTACGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCAA ATATGGCCTC ACAGGTGCAT 
CATAGCCGCT CCAACCCCAC CGATATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGCGGC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTACCT 
CTGGTGCACC CTCCGTCCCA TAGCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT 
GGCTATGAGG TGGGCCAGCG GAAGCGCCTC ATCTCCTCGG TGGAGGACTT CACCGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( NM_001318827.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305756.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 6, mRNA.

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CloneID	OHu61159
Clone ID Related Accession (Same CDS sequence)	NM_001318827.1
Accession Version	NM_001318827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tuberin isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC402106.1, AK299343.1, AK295672.1 and CA427637.1. On Jan 14, 2016 this sequence version replaced XM_011522641.1. Transcript Variant: This variant (6) lacks three alternate in-frame exons in the coding region, compared to variant 1. It encodes isoform 6, which lacks three separate internal in-frame segments and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGGGCGA GCGTTTGGGG 
GTCCTCAGAG CCCTCTTCTT TAAGGTCATC AAGGATTACC CTTCCAACGA AGACCTTCAC 
GAAAGGCTGG AGGTTTTCAA GGCCCTCACA GACAATGGGA GACACATCAC CTACTTGGAG 
GAAGAGCTGG CTGACTTTGT CCTGCAGTGG ATGGATGTTG GCTTGTCCTC GGAATTCCTT 
CTGGTGCTGG TGAACTTGGT CAAATTCAAT AGCTGTTACC TCGACGAGTA CATCGCAAGG 
ATGGTTCAGA TGATCTGTCT GCTGTGCGTC CGGACCGCGT CCTCTGTGGA CATAGAGGTC 
TCCCTGCAGG TGCTGGACGC CGTGGTCTGC TACAACTGCC TGCCGGCTGA GAGCCTCCCG 
CTGTTCATCG TTACCCTCTG TCGCACCATC AACGTCAAGG AGCTCTGCGA GCCTTGCTGG 
AAGCTGATGC GGAACCTCCT TGGCACCCAC CTGGGCCACA GCGCCATCTA CAACATGTGC 
CACCTCATGG AGGACAGAGC CTACATGGAG GACGCGCCCC TGCTGAGAGG AGCCGTGTTT 
TTTGTGGGCA TGGCTCTCTG GGGAGCCCAC CGGCTCTATT CTCTCAGGAA CTCGCCGACA 
TCTGTGTTGC CATCATTTTA CCAGGCCATG GCATGTCCGA ACGAGGTGGT GTCCTATGAG 
ATCGTCCTGT CCATCACCAG GCTCATCAAG AAGTATAGGA AGGAGCTCCA GGTGGTGGCG 
TGGGACATTC TGCTGAACAT CATCGAACGG CTCCTTCAGC AGCTCCAGAC CTTGGACAGC 
CCGGAGCTCA GGACCATCGT CCATGACCTG TTGACCACGG TGGAGGAGCT GTGTGACCAG 
AACGAGTTCC ACGGGTCTCA GGAGAGATAC TTTGAACTGG TGGAGAGATG TGCGGACCAG 
AGGCCTGAGT CCTCCCTCCT GAACCTGATC TCCTATAGAG CGCAGTCCAT CCACCCGGCC 
AAGGACGGCT GGATTCAGAA CCTGCAGGCG CTGATGGAGA GATTCTTCAG GAGCGAGTCC 
CGAGGCGCCG TGCGCATCAA GGTGCTGGAC GTGCTGTCCT TTGTGCTGCT CATCAACAGG 
CAGTTCTATG AGGAGGAGCT GATTAACTCA GTGGTCATCT CGCAGCTCTC CCACATCCCC 
GAGGATAAAG ACCACCAGGT CCGAAAGCTG GCCACCCAGT TGCTGGTGGA CCTGGCAGAG 
GGCTGCCACA CACACCACTT CAACAGCCTG CTGGACATCA TCGAGAAGGT GATGGCCCGC 
TCCCTCTCCC CACCCCCGGA GCTGGAAGAA AGGGATGTGG CCGCATACTC GGCCTCCTTG 
GAGGATGTGA AGACAGCCGT CCTGGGGCTT CTGGTCATCC TTCAGACCAA GCTGTACACC 
CTGCCTGCAA GCCACGCCAC GCGTGTGTAT GAGATGCTGG TCAGCCACAT TCAGCTCCAC 
TACAAGCACA GCTACACCCT GCCAATCGCG AGCAGCATCC GGCTGCAGGC CTTTGACTTC 
CTGTTGCTGC TGCGGGCCGA CTCACTGCAC CGCCTGGGCC TGCCCAACAA GGATGGAGTC 
GTGCGGTTCA GCCCCTACTG CGTCTGCGAC TACATGGAGC CAGAGAGAGG CTCTGAGAAG 
AAGACCAGCG GCCCCCTTTC TCCTCCCACA GGGCCTCCTG GCCCGGCGCC TGCAGGCCCC 
GCCGTGCGGC TGGGGTCCGT GCCCTACTCC CTGCTCTTCC GCGTCCTGCT GCAGTGCTTG 
AAGCAGGAGT CTGACTGGAA GGTGCTGAAG CTGGTTCTGG GCAGGCTGCC TGAGTCCCTG 
CGCTATAAAG TGCTCATCTT TACTTCCCCT TGCAGTGTGG ACCAGCTGTG CTCTGCTCTC 
TGCTCCATGC TTTCAGGCCC AAAGACACTG GAGCGGCTCC GAGGCGCCCC AGAAGGCTTC 
TCCAGAACTG ACTTGCACCT GGCCGTGGTT CCAGTGCTGA CAGCATTAAT CTCTTACCAT 
AACTACCTGG ACAAAACCAA ACAGCGCGAG ATGGTCTACT GCCTGGAGCA GGGCCTCATC 
CACCGCTGTG CCAGCCAGTG CGTCGTGGCC TTGTCCATCT GCAGCGTGGA GATGCCTGAC 
ATCATCATCA AGGCGCTGCC TGTTCTGGTG GTGAAGCTCA CGCACATCTC AGCCACAGCC 
AGCATGGCCG TCCCACTGCT GGAGTTCCTG TCCACTCTGG CCAGGCTGCC GCACCTCTAC 
AGGAACTTTG CCGCGGAGCA GTATGCCAGT GTGTTCGCCA TCTCCCTGCC GTACACCAAC 
CCCTCCAAGT TTAATCAGTA CATCGTGTGT CTGGCCCATC ACGTCATAGC CATGTGGTTC 
ATCAGGTGCC GCCTGCCCTT CCGGAAGGAT TTTGTCCCTT TCATCACTAA GGGCCTGCGG 
TCCAATGTCC TCTTGTCTTT TGATGACACC CCCGAGAAGG ACAGCTTCAG GGCCCGGAGT 
ACTAGTCTCA ACGAGAGACC CAAGAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305756.1
CDS	201..5315
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	NM_001318827.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318827.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGGGCGA GCGTTTGGGG 
GTCCTCAGAG CCCTCTTCTT TAAGGTCATC AAGGATTACC CTTCCAACGA AGACCTTCAC 
GAAAGGCTGG AGGTTTTCAA GGCCCTCACA GACAATGGGA GACACATCAC CTACTTGGAG 
GAAGAGCTGG CTGACTTTGT CCTGCAGTGG ATGGATGTTG GCTTGTCCTC GGAATTCCTT 
CTGGTGCTGG TGAACTTGGT CAAATTCAAT AGCTGTTACC TCGACGAGTA CATCGCAAGG 
ATGGTTCAGA TGATCTGTCT GCTGTGCGTC CGGACCGCGT CCTCTGTGGA CATAGAGGTC 
TCCCTGCAGG TGCTGGACGC CGTGGTCTGC TACAACTGCC TGCCGGCTGA GAGCCTCCCG 
CTGTTCATCG TTACCCTCTG TCGCACCATC AACGTCAAGG AGCTCTGCGA GCCTTGCTGG 
AAGCTGATGC GGAACCTCCT TGGCACCCAC CTGGGCCACA GCGCCATCTA CAACATGTGC 
CACCTCATGG AGGACAGAGC CTACATGGAG GACGCGCCCC TGCTGAGAGG AGCCGTGTTT 
TTTGTGGGCA TGGCTCTCTG GGGAGCCCAC CGGCTCTATT CTCTCAGGAA CTCGCCGACA 
TCTGTGTTGC CATCATTTTA CCAGGCCATG GCATGTCCGA ACGAGGTGGT GTCCTATGAG 
ATCGTCCTGT CCATCACCAG GCTCATCAAG AAGTATAGGA AGGAGCTCCA GGTGGTGGCG 
TGGGACATTC TGCTGAACAT CATCGAACGG CTCCTTCAGC AGCTCCAGAC CTTGGACAGC 
CCGGAGCTCA GGACCATCGT CCATGACCTG TTGACCACGG TGGAGGAGCT GTGTGACCAG 
AACGAGTTCC ACGGGTCTCA GGAGAGATAC TTTGAACTGG TGGAGAGATG TGCGGACCAG 
AGGCCTGAGT CCTCCCTCCT GAACCTGATC TCCTATAGAG CGCAGTCCAT CCACCCGGCC 
AAGGACGGCT GGATTCAGAA CCTGCAGGCG CTGATGGAGA GATTCTTCAG GAGCGAGTCC 
CGAGGCGCCG TGCGCATCAA GGTGCTGGAC GTGCTGTCCT TTGTGCTGCT CATCAACAGG 
CAGTTCTATG AGGAGGAGCT GATTAACTCA GTGGTCATCT CGCAGCTCTC CCACATCCCC 
GAGGATAAAG ACCACCAGGT CCGAAAGCTG GCCACCCAGT TGCTGGTGGA CCTGGCAGAG 
GGCTGCCACA CACACCACTT CAACAGCCTG CTGGACATCA TCGAGAAGGT GATGGCCCGC 
TCCCTCTCCC CACCCCCGGA GCTGGAAGAA AGGGATGTGG CCGCATACTC GGCCTCCTTG 
GAGGATGTGA AGACAGCCGT CCTGGGGCTT CTGGTCATCC TTCAGACCAA GCTGTACACC 
CTGCCTGCAA GCCACGCCAC GCGTGTGTAT GAGATGCTGG TCAGCCACAT TCAGCTCCAC 
TACAAGCACA GCTACACCCT GCCAATCGCG AGCAGCATCC GGCTGCAGGC CTTTGACTTC 
CTGTTGCTGC TGCGGGCCGA CTCACTGCAC CGCCTGGGCC TGCCCAACAA GGATGGAGTC 
GTGCGGTTCA GCCCCTACTG CGTCTGCGAC TACATGGAGC CAGAGAGAGG CTCTGAGAAG 
AAGACCAGCG GCCCCCTTTC TCCTCCCACA GGGCCTCCTG GCCCGGCGCC TGCAGGCCCC 
GCCGTGCGGC TGGGGTCCGT GCCCTACTCC CTGCTCTTCC GCGTCCTGCT GCAGTGCTTG 
AAGCAGGAGT CTGACTGGAA GGTGCTGAAG CTGGTTCTGG GCAGGCTGCC TGAGTCCCTG 
CGCTATAAAG TGCTCATCTT TACTTCCCCT TGCAGTGTGG ACCAGCTGTG CTCTGCTCTC 
TGCTCCATGC TTTCAGGCCC AAAGACACTG GAGCGGCTCC GAGGCGCCCC AGAAGGCTTC 
TCCAGAACTG ACTTGCACCT GGCCGTGGTT CCAGTGCTGA CAGCATTAAT CTCTTACCAT 
AACTACCTGG ACAAAACCAA ACAGCGCGAG ATGGTCTACT GCCTGGAGCA GGGCCTCATC 
CACCGCTGTG CCAGCCAGTG CGTCGTGGCC TTGTCCATCT GCAGCGTGGA GATGCCTGAC 
ATCATCATCA AGGCGCTGCC TGTTCTGGTG GTGAAGCTCA CGCACATCTC AGCCACAGCC 
AGCATGGCCG TCCCACTGCT GGAGTTCCTG TCCACTCTGG CCAGGCTGCC GCACCTCTAC 
AGGAACTTTG CCGCGGAGCA GTATGCCAGT GTGTTCGCCA TCTCCCTGCC GTACACCAAC 
CCCTCCAAGT TTAATCAGTA CATCGTGTGT CTGGCCCATC ACGTCATAGC CATGTGGTTC 
ATCAGGTGCC GCCTGCCCTT CCGGAAGGAT TTTGTCCCTT TCATCACTAA GGGCCTGCGG 
TCCAATGTCC TCTTGTCTTT TGATGACACC CCCGAGAAGG ACAGCTTCAG GGCCCGGAGT 
ACTAGTCTCA ACGAGAGACC CAAGAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_011522636.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520938.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OHu61152
Clone ID Related Accession (Same CDS sequence)	XM_011522636.2
Accession Version	XM_011522636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGGTATCGC CTCTCAGAGG 
GAAGCGGTTG GCTGCAGAGC GCCACTCTGC CTCATAGACT CCGCCGTGGT CATGGAGGAG 
GGAAGTCCGG GCGAGGTTCC TGTGCTGGTG GAGCCCCCAG GGTTGGAGGA CGTTGAGGCA 
GCGCTAGGCA TGGACAGGCG CACGGATGCC TACAGCAGGT CGTCCTCAGT CTCCAGCCAG 
GAGGAGAAGT CGCTCCACGC GGAGGAGCTG GTTGGCAGGG GCATCCCCAT CGAGCGAGTC 
GTCTCCTCGG AGGGTGGCCG GCCCTCTGTG GACCTCTCCT TCCAGCCCTC GCAGCCCCTG 
AGCAAGTCCA GCTCCTCTCC CGAGCTGCAG ACTCTGCAGG ACATCCTCGG GGACCCTGGG 
GACAAGGCCG ACGTGGGCCG GCTGAGCCCT GAGGTTAAGG CCCGGTCACA GTCAGGGACC 
CTGGACGGGG AAAGTGCTGC CTGGTCGGCC TCGGGCGAAG ACAGTCGGGG CCAGCCCGAG 
GGTCCCTTGC CTTCCAGCTC CCCCCGCTCG CCCAGTGGCC TCCGGCCCCG AGGTTACACC 
ATCTCCGACT CGGCCCCATC ACGCAGGGGC AAGAGAGTAG AGAGGGACGC CTTAAAGAGC 
AGAGCCACAG CCTCCAATGC AGAGAAAGTG CCAGGCATCA ACCCCAGTTT CGTGTTCCTG 
CAGCTCTACC ATTCCCCCTT CTTTGGCGAC GAGTCAAACA AGCCAATCCT GCTGCCCAAT 
GAGTCACAGT CCTTTGAGCG GTCGGTGCAG CTCCTCGACC AGATCCCATC ATACGACACC 
CACAAGATCG CCGTCCTGTA TGTTGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAATGAGC ATGGCTCCTA CAGGTACACG GAGTTCCTGA CGGGCCTGGG CCGGCTCATC 
GAGCTGAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGAG GCCTGGACGT GTGTGGTGAG 
GACGGCCAGT TCACCTACTG CTGGCACGAT GACATCATGC AAGCCGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGCG ACAAGAAGCG CCACCTGGGC 
AACGACTTTG TGTCCATTGT CTACAATGAC TCCGGTGAGG ACTTCAAGCT TGGCACCATC 
AAGGGCCAGT TCAACTTTGT CCACGTGATC GTCACCCCGC TGGACTACGA GTGCAACCTG 
GTGTCCCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ACACCAGCGT GGCCAAGATC 
GTGTCTGACC GCAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC AAATATGGCC 
TCACAGGTGC ATCATAGCCG CTCCAACCCC ACCGATATCT ACCCCTCCAA GTGGATTGCC 
CGGCTCCGCC ACATCAAGCG GCTCCGCCAG CGGATCTGCG AGGAAGCCGC CTACTCCAAC 
CCCAGCCTAC CTCTGGTGCA CCCTCCGTCC CATAGCAAAG CCCCTGCACA GACTCCAGCC 
GAGCCCACAC CTGGCTATGA GGTGGGCCAG CGGAAGCGCC TCATCTCCTC GGTGGAGGAC 
TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520938.1
CDS	88..5565
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWAGIASQREAVGCRAPLCLIDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_011522636.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522636.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ATGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTAGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GCTGCAGTCC GGCCCGGAGT CGAGCTCCAG CCCCGGGGTG 
CATGTGAGAC AGACCAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGATCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACG TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAACCTGA GAAGGCCTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACCTGG CGGCCTATGT GCCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCTAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCACTGT CGGTGCCGGC AGCCAGCACG 
GCCAAACCCC CTCCTCTGCC TCGCTCCAAC ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGGTATCGC CTCTCAGAGG 
GAAGCGGTTG GCTGCAGAGC GCCACTCTGC CTCATAGACT CCGCCGTGGT CATGGAGGAG 
GGAAGTCCGG GCGAGGTTCC TGTGCTGGTG GAGCCCCCAG GGTTGGAGGA CGTTGAGGCA 
GCGCTAGGCA TGGACAGGCG CACGGATGCC TACAGCAGGT CGTCCTCAGT CTCCAGCCAG 
GAGGAGAAGT CGCTCCACGC GGAGGAGCTG GTTGGCAGGG GCATCCCCAT CGAGCGAGTC 
GTCTCCTCGG AGGGTGGCCG GCCCTCTGTG GACCTCTCCT TCCAGCCCTC GCAGCCCCTG 
AGCAAGTCCA GCTCCTCTCC CGAGCTGCAG ACTCTGCAGG ACATCCTCGG GGACCCTGGG 
GACAAGGCCG ACGTGGGCCG GCTGAGCCCT GAGGTTAAGG CCCGGTCACA GTCAGGGACC 
CTGGACGGGG AAAGTGCTGC CTGGTCGGCC TCGGGCGAAG ACAGTCGGGG CCAGCCCGAG 
GGTCCCTTGC CTTCCAGCTC CCCCCGCTCG CCCAGTGGCC TCCGGCCCCG AGGTTACACC 
ATCTCCGACT CGGCCCCATC ACGCAGGGGC AAGAGAGTAG AGAGGGACGC CTTAAAGAGC 
AGAGCCACAG CCTCCAATGC AGAGAAAGTG CCAGGCATCA ACCCCAGTTT CGTGTTCCTG 
CAGCTCTACC ATTCCCCCTT CTTTGGCGAC GAGTCAAACA AGCCAATCCT GCTGCCCAAT 
GAGTCACAGT CCTTTGAGCG GTCGGTGCAG CTCCTCGACC AGATCCCATC ATACGACACC 
CACAAGATCG CCGTCCTGTA TGTTGGAGAA GGCCAGAGCA ACAGCGAGCT CGCCATCCTG 
TCCAATGAGC ATGGCTCCTA CAGGTACACG GAGTTCCTGA CGGGCCTGGG CCGGCTCATC 
GAGCTGAAGG ACTGCCAGCC GGACAAGGTG TACCTGGGAG GCCTGGACGT GTGTGGTGAG 
GACGGCCAGT TCACCTACTG CTGGCACGAT GACATCATGC AAGCCGTCTT CCACATCGCC 
ACCCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGCG ACAAGAAGCG CCACCTGGGC 
AACGACTTTG TGTCCATTGT CTACAATGAC TCCGGTGAGG ACTTCAAGCT TGGCACCATC 
AAGGGCCAGT TCAACTTTGT CCACGTGATC GTCACCCCGC TGGACTACGA GTGCAACCTG 
GTGTCCCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ACACCAGCGT GGCCAAGATC 
GTGTCTGACC GCAACCTGCC CTTCGTGGCC CGCCAGATGG CCCTGCACGC AAATATGGCC 
TCACAGGTGC ATCATAGCCG CTCCAACCCC ACCGATATCT ACCCCTCCAA GTGGATTGCC 
CGGCTCCGCC ACATCAAGCG GCTCCGCCAG CGGATCTGCG AGGAAGCCGC CTACTCCAAC 
CCCAGCCTAC CTCTGGTGCA CCCTCCGTCC CATAGCAAAG CCCCTGCACA GACTCCAGCC 
GAGCCCACAC CTGGCTATGA GGTGGGCCAG CGGAAGCGCC TCATCTCCTC GGTGGAGGAC 
TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_011522637.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520939.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

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CloneID	OHu61153
Clone ID Related Accession (Same CDS sequence)	XM_011522637.2
Accession Version	XM_011522637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATA CAGACGTCCC TCACCAGTGC CAGCTTGGGG 
TCTGCAGATG AGAACTCCGT GGCCCAGGCT GACGATAGCC TGAAAAACCT CCACCTGGAG 
CTCACGGAAA CCTGTCTGGA CATGATGGCT CGATACGTCT TCTCCAACTT CACGGCTGTC 
CCGAAGAGGT CTCCTGTGGG CGAGTTCCTC CTAGCGGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC TGTGACGACA AGCGTGGGAA CCGGGACCCG GTCGTTACTA 
GGCCTGGACT CGGGGGAGCT GCAGTCCGGC CCGGAGTCGA GCTCCAGCCC CGGGGTGCAT 
GTGAGACAGA CCAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGATCGGGT CCGTTCCATG TCGGGGGGCC ATGGTCTTCG AGTTGGCGCC 
CTGGACGTGC CGGCCTCCCA GTTCCTGGGC AGTGCCACTT CTCCAGGACC ACGGACTGCA 
CCAGCCGCGA AACCTGAGAA GGCCTCAGCT GGCACCCGGG TTCCTGTGCA GGAGAAGACG 
AACCTGGCGG CCTATGTGCC CCTGCTGACC CAGGGCTGGG CGGAGATCCT GGTCCGGAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC TTTCTCCTCG 
GACATCAACA ACATGCCCCT GCAGGAGCTG TCTAACGCCC TCATGGCGGC TGAGCGCTTC 
AAGGAGCACC GGGACACAGC CCTGTACAAG TCACTGTCGG TGCCGGCAGC CAGCACGGCC 
AAACCCCCTC CTCTGCCTCG CTCCAACACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG GTATCGCCTC TCAGAGGGAA 
GCGGTTGGCT GCAGAGCGCC ACTCTGCCTC ATAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520939.1
CDS	88..5562
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWAGIASQREAVGCRAPLCLIDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_011522637.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522637.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGGATA CAGACGTCCC TCACCAGTGC CAGCTTGGGG 
TCTGCAGATG AGAACTCCGT GGCCCAGGCT GACGATAGCC TGAAAAACCT CCACCTGGAG 
CTCACGGAAA CCTGTCTGGA CATGATGGCT CGATACGTCT TCTCCAACTT CACGGCTGTC 
CCGAAGAGGT CTCCTGTGGG CGAGTTCCTC CTAGCGGGTG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC TGTGACGACA AGCGTGGGAA CCGGGACCCG GTCGTTACTA 
GGCCTGGACT CGGGGGAGCT GCAGTCCGGC CCGGAGTCGA GCTCCAGCCC CGGGGTGCAT 
GTGAGACAGA CCAAGGAGGC GCCGGCCAAG CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC 
CGTGGGGCCC GGGATCGGGT CCGTTCCATG TCGGGGGGCC ATGGTCTTCG AGTTGGCGCC 
CTGGACGTGC CGGCCTCCCA GTTCCTGGGC AGTGCCACTT CTCCAGGACC ACGGACTGCA 
CCAGCCGCGA AACCTGAGAA GGCCTCAGCT GGCACCCGGG TTCCTGTGCA GGAGAAGACG 
AACCTGGCGG CCTATGTGCC CCTGCTGACC CAGGGCTGGG CGGAGATCCT GGTCCGGAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC TTTCTCCTCG 
GACATCAACA ACATGCCCCT GCAGGAGCTG TCTAACGCCC TCATGGCGGC TGAGCGCTTC 
AAGGAGCACC GGGACACAGC CCTGTACAAG TCACTGTCGG TGCCGGCAGC CAGCACGGCC 
AAACCCCCTC CTCTGCCTCG CTCCAACACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG GTATCGCCTC TCAGAGGGAA 
GCGGTTGGCT GCAGAGCGCC ACTCTGCCTC ATAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_011522639.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520941.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OHu61155
Clone ID Related Accession (Same CDS sequence)	XM_011522639.2
Accession Version	XM_011522639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGGTATCG CCTCTCAGAG GGAAGCGGTT 
GGCTGCAGAG CGCCACTCTG CCTCATAGAC TCCGCCGTGG TCATGGAGGA GGGAAGTCCG 
GGCGAGGTTC CTGTGCTGGT GGAGCCCCCA GGGTTGGAGG ACGTTGAGGC AGCGCTAGGC 
ATGGACAGGC GCACGGATGC CTACAGCAGG TCGTCCTCAG TCTCCAGCCA GGAGGAGAAG 
TCGCTCCACG CGGAGGAGCT GGTTGGCAGG GGCATCCCCA TCGAGCGAGT CGTCTCCTCG 
GAGGGTGGCC GGCCCTCTGT GGACCTCTCC TTCCAGCCCT CGCAGCCCCT GAGCAAGTCC 
AGCTCCTCTC CCGAGCTGCA GACTCTGCAG GACATCCTCG GGGACCCTGG GGACAAGGCC 
GACGTGGGCC GGCTGAGCCC TGAGGTTAAG GCCCGGTCAC AGTCAGGGAC CCTGGACGGG 
GAAAGTGCTG CCTGGTCGGC CTCGGGCGAA GACAGTCGGG GCCAGCCCGA GGGTCCCTTG 
CCTTCCAGCT CCCCCCGCTC GCCCAGTGGC CTCCGGCCCC GAGGTTACAC CATCTCCGAC 
TCGGCCCCAT CACGCAGGGG CAAGAGAGTA GAGAGGGACG CCTTAAAGAG CAGAGCCACA 
GCCTCCAATG CAGAGAAAGT GCCAGGCATC AACCCCAGTT TCGTGTTCCT GCAGCTCTAC 
CATTCCCCCT TCTTTGGCGA CGAGTCAAAC AAGCCAATCC TGCTGCCCAA TGAGTCACAG 
TCCTTTGAGC GGTCGGTGCA GCTCCTCGAC CAGATCCCAT CATACGACAC CCACAAGATC 
GCCGTCCTGT ATGTTGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAATGAG 
CATGGCTCCT ACAGGTACAC GGAGTTCCTG ACGGGCCTGG GCCGGCTCAT CGAGCTGAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGA GGCCTGGACG TGTGTGGTGA GGACGGCCAG 
TTCACCTACT GCTGGCACGA TGACATCATG CAAGCCGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGC GACAAGAAGC GCCACCTGGG CAACGACTTT 
GTGTCCATTG TCTACAATGA CTCCGGTGAG GACTTCAAGC TTGGCACCAT CAAGGGCCAG 
TTCAACTTTG TCCACGTGAT CGTCACCCCG CTGGACTACG AGTGCAACCT GGTGTCCCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GACACCAGCG TGGCCAAGAT CGTGTCTGAC 
CGCAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CAAATATGGC CTCACAGGTG 
CATCATAGCC GCTCCAACCC CACCGATATC TACCCCTCCA AGTGGATTGC CCGGCTCCGC 
CACATCAAGC GGCTCCGCCA GCGGATCTGC GAGGAAGCCG CCTACTCCAA CCCCAGCCTA 
CCTCTGGTGC ACCCTCCGTC CCATAGCAAA GCCCCTGCAC AGACTCCAGC CGAGCCCACA 
CCTGGCTATG AGGTGGGCCA GCGGAAGCGC CTCATCTCCT CGGTGGAGGA CTTCACCGAG 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520941.1
CDS	88..5436
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWAGIASQREAVGCRAPLCLIDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_011522639.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522639.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGGTATCG CCTCTCAGAG GGAAGCGGTT 
GGCTGCAGAG CGCCACTCTG CCTCATAGAC TCCGCCGTGG TCATGGAGGA GGGAAGTCCG 
GGCGAGGTTC CTGTGCTGGT GGAGCCCCCA GGGTTGGAGG ACGTTGAGGC AGCGCTAGGC 
ATGGACAGGC GCACGGATGC CTACAGCAGG TCGTCCTCAG TCTCCAGCCA GGAGGAGAAG 
TCGCTCCACG CGGAGGAGCT GGTTGGCAGG GGCATCCCCA TCGAGCGAGT CGTCTCCTCG 
GAGGGTGGCC GGCCCTCTGT GGACCTCTCC TTCCAGCCCT CGCAGCCCCT GAGCAAGTCC 
AGCTCCTCTC CCGAGCTGCA GACTCTGCAG GACATCCTCG GGGACCCTGG GGACAAGGCC 
GACGTGGGCC GGCTGAGCCC TGAGGTTAAG GCCCGGTCAC AGTCAGGGAC CCTGGACGGG 
GAAAGTGCTG CCTGGTCGGC CTCGGGCGAA GACAGTCGGG GCCAGCCCGA GGGTCCCTTG 
CCTTCCAGCT CCCCCCGCTC GCCCAGTGGC CTCCGGCCCC GAGGTTACAC CATCTCCGAC 
TCGGCCCCAT CACGCAGGGG CAAGAGAGTA GAGAGGGACG CCTTAAAGAG CAGAGCCACA 
GCCTCCAATG CAGAGAAAGT GCCAGGCATC AACCCCAGTT TCGTGTTCCT GCAGCTCTAC 
CATTCCCCCT TCTTTGGCGA CGAGTCAAAC AAGCCAATCC TGCTGCCCAA TGAGTCACAG 
TCCTTTGAGC GGTCGGTGCA GCTCCTCGAC CAGATCCCAT CATACGACAC CCACAAGATC 
GCCGTCCTGT ATGTTGGAGA AGGCCAGAGC AACAGCGAGC TCGCCATCCT GTCCAATGAG 
CATGGCTCCT ACAGGTACAC GGAGTTCCTG ACGGGCCTGG GCCGGCTCAT CGAGCTGAAG 
GACTGCCAGC CGGACAAGGT GTACCTGGGA GGCCTGGACG TGTGTGGTGA GGACGGCCAG 
TTCACCTACT GCTGGCACGA TGACATCATG CAAGCCGTCT TCCACATCGC CACCCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGC GACAAGAAGC GCCACCTGGG CAACGACTTT 
GTGTCCATTG TCTACAATGA CTCCGGTGAG GACTTCAAGC TTGGCACCAT CAAGGGCCAG 
TTCAACTTTG TCCACGTGAT CGTCACCCCG CTGGACTACG AGTGCAACCT GGTGTCCCTG 
CAGTGCAGGA AAGACATGGA GGGCCTTGTG GACACCAGCG TGGCCAAGAT CGTGTCTGAC 
CGCAACCTGC CCTTCGTGGC CCGCCAGATG GCCCTGCACG CAAATATGGC CTCACAGGTG 
CATCATAGCC GCTCCAACCC CACCGATATC TACCCCTCCA AGTGGATTGC CCGGCTCCGC 
CACATCAAGC GGCTCCGCCA GCGGATCTGC GAGGAAGCCG CCTACTCCAA CCCCAGCCTA 
CCTCTGGTGC ACCCTCCGTC CCATAGCAAA GCCCCTGCAC AGACTCCAGC CGAGCCCACA 
CCTGGCTATG AGGTGGGCCA GCGGAAGCGC CTCATCTCCT CGGTGGAGGA CTTCACCGAG 
TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_011522640.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520942.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

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CloneID	OHu61156
Clone ID Related Accession (Same CDS sequence)	XM_011522640.2
Accession Version	XM_011522640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GGTATCGCCT CTCAGAGGGA AGCGGTTGGC 
TGCAGAGCGC CACTCTGCCT CATAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCGGGC 
GAGGTTCCTG TGCTGGTGGA GCCCCCAGGG TTGGAGGACG TTGAGGCAGC GCTAGGCATG 
GACAGGCGCA CGGATGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG 
CTCCACGCGG AGGAGCTGGT TGGCAGGGGC ATCCCCATCG AGCGAGTCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC 
TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA 
AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAA GAGAGTAGAG AGGGACGCCT TAAAGAGCAG AGCCACAGCC 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA CGACTTTGTG 
TCCATTGTCT ACAATGACTC CGGTGAGGAC TTCAAGCTTG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTACGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCAA ATATGGCCTC ACAGGTGCAT 
CATAGCCGCT CCAACCCCAC CGATATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGCGGC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTACCT 
CTGGTGCACC CTCCGTCCCA TAGCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT 
GGCTATGAGG TGGGCCAGCG GAAGCGCCTC ATCTCCTCGG TGGAGGACTT CACCGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520942.1
CDS	88..5433
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWAGIASQREAVGCRAPLCLIDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_011522640.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522640.2

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAT 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC CCGGGGTGCA TGTGAGACAG 
ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACGTG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACCTGGCG 
GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG CCAGCACGGC CAAACCCCCT 
CCTCTGCCTC GCTCCAACAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GGTATCGCCT CTCAGAGGGA AGCGGTTGGC 
TGCAGAGCGC CACTCTGCCT CATAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCGGGC 
GAGGTTCCTG TGCTGGTGGA GCCCCCAGGG TTGGAGGACG TTGAGGCAGC GCTAGGCATG 
GACAGGCGCA CGGATGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG 
CTCCACGCGG AGGAGCTGGT TGGCAGGGGC ATCCCCATCG AGCGAGTCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC 
TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA 
AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAA GAGAGTAGAG AGGGACGCCT TAAAGAGCAG AGCCACAGCC 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA CGACTTTGTG 
TCCATTGTCT ACAATGACTC CGGTGAGGAC TTCAAGCTTG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTACGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCAA ATATGGCCTC ACAGGTGCAT 
CATAGCCGCT CCAACCCCAC CGATATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGCGGC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTACCT 
CTGGTGCACC CTCCGTCCCA TAGCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT 
GGCTATGAGG TGGGCCAGCG GAAGCGCCTC ATCTCCTCGG TGGAGGACTT CACCGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005255529.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255586.2 Homo sapiens tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

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CloneID	OHu61157
Clone ID Related Accession (Same CDS sequence)	NM_021055.2 , XM_005255529.4
Accession Version	XM_005255529.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255529.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255586.2
CDS	88..5382
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_005255529.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255529.4

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCCG CCGTGGTCAT GGAGGAGGGA 
AGTCCGGGCG AGGTTCCTGT GCTGGTGGAG CCCCCAGGGT TGGAGGACGT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GGCAGGGGCA TCCCCATCGA GCGAGTCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACT CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCGGCCTCG GGCGAAGACA GTCGGGGCCA GCCCGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG CAGGGGCAAG AGAGTAGAGA GGGACGCCTT AAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCA GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AATGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAGGACT GCCAGCCGGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAC 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCCACC 
CTGATGCCCA CCAAGGACGT GGACAAGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAC 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGTGAGGACT TCAAGCTTGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTACGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCC TGCACGCAAA TATGGCCTCA 
CAGGTGCATC ATAGCCGCTC CAACCCCACC GATATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGCGGCT CCGCCAGCGG ATCTGCGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTACCTC TGGTGCACCC TCCGTCCCAT AGCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTATGAGGT GGGCCAGCGG AAGCGCCTCA TCTCCTCGGT GGAGGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005255531.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255588.2 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

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CloneID	OHu61158
Clone ID Related Accession (Same CDS sequence)	NM_001363528.1 , XM_005255531.4
Accession Version	XM_005255531.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	tuberin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255531.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCGGGC 
GAGGTTCCTG TGCTGGTGGA GCCCCCAGGG TTGGAGGACG TTGAGGCAGC GCTAGGCATG 
GACAGGCGCA CGGATGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG 
CTCCACGCGG AGGAGCTGGT TGGCAGGGGC ATCCCCATCG AGCGAGTCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC 
TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA 
AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAA GAGAGTAGAG AGGGACGCCT TAAAGAGCAG AGCCACAGCC 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA CGACTTTGTG 
TCCATTGTCT ACAATGACTC CGGTGAGGAC TTCAAGCTTG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTACGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCAA ATATGGCCTC ACAGGTGCAT 
CATAGCCGCT CCAACCCCAC CGATATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGCGGC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTACCT 
CTGGTGCACC CTCCGTCCCA TAGCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT 
GGCTATGAGG TGGGCCAGCG GAAGCGCCTC ATCTCCTCGG TGGAGGACTT CACCGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255588.2
CDS	88..5313
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_005255531.4
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255531.4

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT CGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTCTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CGCTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGGCAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAACG AAGACCTTCA CGAAAGGCTG 
GAGGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTGTGCGT CCGGACCGCG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CCGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACCCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ACAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTCAGGA ACTCGCCGAC ATCTGTGTTG 
CCATCATTTT ACCAGGCCAT GGCATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTATAGG AAGGAGCTCC AGGTGGTGGC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCATCG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCTGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGATGGCCCG CTCCCTCTCC 
CCACCCCCGG AGCTGGAAGA AAGGGATGTG GCCGCATACT CGGCCTCCTT GGAGGATGTG 
AAGACAGCCG TCCTGGGGCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGTGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATCGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTGGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCAC AGGGCCTCCT GGCCCGGCGC CTGCAGGCCC CGCCGTGCGG 
CTGGGGTCCG TGCCCTACTC CCTGCTCTTC CGCGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CTGAGTCCCT GCGCTATAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACTTGCACC TGGCCGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CTGTTCTGGT GGTGAAGCTC ACGCACATCT CAGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GATGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTAGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGCTGCAGTC CGGCCCGGAG TCGAGCTCCA GCCCCGGGGT GCATGTGAGA 
CAGACCAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGATC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
GTGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAACCTG AGAAGGCCTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACCTG 
GCGGCCTATG TGCCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC CTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCTAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCACTG TCGGTGCCGG CAGCCAGCAC GGCCAAACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCCGTGGTCA TGGAGGAGGG AAGTCCGGGC 
GAGGTTCCTG TGCTGGTGGA GCCCCCAGGG TTGGAGGACG TTGAGGCAGC GCTAGGCATG 
GACAGGCGCA CGGATGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG 
CTCCACGCGG AGGAGCTGGT TGGCAGGGGC ATCCCCATCG AGCGAGTCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC 
TCCTCTCCCG AGCTGCAGAC TCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA 
AGTGCTGCCT GGTCGGCCTC GGGCGAAGAC AGTCGGGGCC AGCCCGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GCAGGGGCAA GAGAGTAGAG AGGGACGCCT TAAAGAGCAG AGCCACAGCC 
TCCAATGCAG AGAAAGTGCC AGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAATGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAGGAC 
TGCCAGCCGG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA CGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCCAC CCTGATGCCC 
ACCAAGGACG TGGACAAGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA CGACTTTGTG 
TCCATTGTCT ACAATGACTC CGGTGAGGAC TTCAAGCTTG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTACGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC CTGCACGCAA ATATGGCCTC ACAGGTGCAT 
CATAGCCGCT CCAACCCCAC CGATATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGCGGC TCCGCCAGCG GATCTGCGAG GAAGCCGCCT ACTCCAACCC CAGCCTACCT 
CTGGTGCACC CTCCGTCCCA TAGCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT 
GGCTATGAGG TGGGCCAGCG GAAGCGCCTC ATCTCCTCGG TGGAGGACTT CACCGAGTTT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_011522638.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520940.2 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OHu86240
Clone ID Related Accession (Same CDS sequence)	XM_011522638.2
Accession Version	XM_011522638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACT CCTGCGGACT ACACATCCCG GCGGCCCATG CGGCCCCGTC ACGTGATGCA 
AGGATCGCCG GCCTTTCCGC CAGAGGGCGG CACAGAACTA CAACTCCCAG CAAGCTCCCA 
AGGCGGCCCT CCGCGCAATG CCGCTACCGG AAGTGCGGGT CGCGCTTCCG GCGGCGTCCC 
GGGGCCAGGG GGGTGCGCCT TTCTCCGCGT CGGGGCGGCC CGGAGCGCGG TGGCGCGGCG 
CGGGAGGGGT TTTCTGGTGC GTCCTGGTCC ACCATGGCCA AACCAACAAG CAAAGATTCA 
GGCTTGAAGG AGAAGTTTAA GATTCTGTTG GGACTGGGAA CACCGAGGCC AAATCCCAGG 
TCTGCAGAGG GTAAACAGAC GGAGTTTATC ATCACCGCGG AAATACTGAG AGAACTGAGC 
ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTCGCAAAA 
ACCAAGAAAT TTGAAGAGGG CGAGCGTTTG GGGGTCCTCA GAGCCCTCTT CTTTAAGGTC 
ATCAAGGATT ACCCTTCCAA CGAAGACCTT CACGAAAGGC TGGAGGTTTT CAAGGCCCTC 
ACAGACAATG GGAGACACAT CACCTACTTG GAGGAAGAGC TGGCTGACTT TGTCCTGCAG 
TGGATGGATG TTGGCTTGTC CTCGGAATTC CTTCTGGTGC TGGTGAACTT GGTCAAATTC 
AATAGCTGTT ACCTCGACGA GTACATCGCA AGGATGGTTC AGATGATCTG TCTGCTGTGC 
GTCCGGACCG CGTCCTCTGT GGACATAGAG GTCTCCCTGC AGGTGCTGGA CGCCGTGGTC 
TGCTACAACT GCCTGCCGGC TGAGAGCCTC CCGCTGTTCA TCGTTACCCT CTGTCGCACC 
ATCAACGTCA AGGAGCTCTG CGAGCCTTGC TGGAAGCTGA TGCGGAACCT CCTTGGCACC 
CACCTGGGCC ACAGCGCCAT CTACAACATG TGCCACCTCA TGGAGGACAG AGCCTACATG 
GAGGACGCGC CCCTGCTGAG AGGAGCCGTG TTTTTTGTGG GCATGGCTCT CTGGGGAGCC 
CACCGGCTCT ATTCTCTCAG GAACTCGCCG ACATCTGTGT TGCCATCATT TTACCAGGCC 
ATGGCATGTC CGAACGAGGT GGTGTCCTAT GAGATCGTCC TGTCCATCAC CAGGCTCATC 
AAGAAGTATA GGAAGGAGCT CCAGGTGGTG GCGTGGGACA TTCTGCTGAA CATCATCGAA 
CGGCTCCTTC AGCAGCTCCA GACCTTGGAC AGCCCGGAGC TCAGGACCAT CGTCCATGAC 
CTGTTGACCA CGGTGGAGGA GCTGTGTGAC CAGAACGAGT TCCACGGGTC TCAGGAGAGA 
TACTTTGAAC TGGTGGAGAG ATGTGCGGAC CAGAGGCCTG AGTCCTCCCT CCTGAACCTG 
ATCTCCTATA GAGCGCAGTC CATCCACCCG GCCAAGGACG GCTGGATTCA GAACCTGCAG 
GCGCTGATGG AGAGATTCTT CAGGAGCGAG TCCCGAGGCG CCGTGCGCAT CAAGGTGCTG 
GACGTGCTGT CCTTTGTGCT GCTCATCAAC AGGCAGTTCT ATGAGGAGGA GCTGATTAAC 
TCAGTGGTCA TCTCGCAGCT CTCCCACATC CCCGAGGATA AAGACCACCA GGTCCGAAAG 
CTGGCCACCC AGTTGCTGGT GGACCTGGCA GAGGGCTGCC ACACACACCA CTTCAACAGC 
CTGCTGGACA TCATCGAGAA GGTGATGGCC CGCTCCCTCT CCCCACCCCC GGAGCTGGAA 
GAAAGGGATG TGGCCGCATA CTCGGCCTCC TTGGAGGATG TGAAGACAGC CGTCCTGGGG 
CTTCTGGTCA TCCTTCAGAC CAAGCTGTAC ACCCTGCCTG CAAGCCACGC CACGCGTGTG 
TATGAGATGC TGGTCAGCCA CATTCAGCTC CACTACAAGC ACAGCTACAC CCTGCCAATC 
GCGAGCAGCA TCCGGCTGCA GGCCTTTGAC TTCCTGTTGC TGCTGCGGGC CGACTCACTG 
CACCGCCTGG GCCTGCCCAA CAAGGATGGA GTCGTGCGGT TCAGCCCCTA CTGCGTCTGC 
GACTACATGG AGCCAGAGAG AGGCTCTGAG AAGAAGACCA GCGGCCCCCT TTCTCCTCCC 
ACAGGGCCTC CTGGCCCGGC GCCTGCAGGC CCCGCCGTGC GGCTGGGGTC CGTGCCCTAC 
TCCCTGCTCT TCCGCGTCCT GCTGCAGTGC TTGAAGCAGG AGTCTGACTG GAAGGTGCTG 
AAGCTGGTTC TGGGCAGGCT GCCTGAGTCC CTGCGCTATA AAGTGCTCAT CTTTACTTCC 
CCTTGCAGTG TGGACCAGCT GTGCTCTGCT CTCTGCTCCA TGCTTTCAGG CCCAAAGACA 
CTGGAGCGGC TCCGAGGCGC CCCAGAAGGC TTCTCCAGAA CTGACTTGCA CCTGGCCGTG 
GTTCCAGTGC TGACAGCATT AATCTCTTAC CATAACTACC TGGACAAAAC CAAACAGCGC 
GAGATGGTCT ACTGCCTGGA GCAGGGCCTC ATCCACCGCT GTGCCAGCCA GTGCGTCGTG 
GCCTTGTCCA TCTGCAGCGT GGAGATGCCT GACATCATCA TCAAGGCGCT GCCTGTTCTG 
GTGGTGAAGC TCACGCACAT CTCAGCCACA GCCAGCATGG CCGTCCCACT GCTGGAGTTC 
CTGTCCACTC TGGCCAGGCT GCCGCACCTC TACAGGAACT TTGCCGCGGA GCAGTATGCC 
AGTGTGTTCG CCATCTCCCT GCCGTACACC AACCCCTCCA AGTTTAATCA GTACATCGTG 
TGTCTGGCCC ATCACGTCAT AGCCATGTGG TTCATCAGGT GCCGCCTGCC CTTCCGGAAG 
GATTTTGTCC CTTTCATCAC TAAGGGCCTG CGGTCCAATG TCCTCTTGTC TTTTGATGAC 
ACCCCCGAGA AGGACAGCTT CAGGGCCCGG AGTACTAGTC TCAACGAGAG ACCCAAGAGT 
CTGAGGATAG CCAGACCCCC CAAACAAGGC TTGAATAACT CTCCACCCGT GAAAGAATTC 
AAGGAGAGCT CTGCAGCCGA GGCCTTCCGG TGCCGCAGCA TCAGTGTGTC TGAACATGTG 
GTCCGCAGCA GGATACAGAC GTCCCTCACC AGTGCCAGCT TGGGGTCTGC AGATGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AACCTCCACC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCTCGATA CGTCTTCTCC AACTTCACGG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTAGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACAAGCGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGGG TGCATGTGAG ACAGACCAAG 
GAGGCGCCGG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAT 
CGGGTCCGTT CCATGTCGGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA CGTGCCGGCC 
TCCCAGTTCC TGGGCAGTGC CACTTCTCCA GGACCACGGA CTGCACCAGC CGCGAAACCT 
GAGAAGGCCT CAGCTGGCAC CCGGGTTCCT GTGCAGGAGA AGACGAACCT GGCGGCCTAT 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCTAA CGCCCTCATG GCGGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCACT GTCGGTGCCG GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTACC AGTCCAGCTG CCAAGGACAG 
CTGCACAGGA GCGTTTCCTG GGCAGGTATC GCCTCTCAGA GGGAAGCGGT TGGCTGCAGA 
GCGCCACTCT GCCTCATAGA CTCCGCCGTG GTCATGGAGG AGGGAAGTCC GGGCGAGGTT 
CCTGTGCTGG TGGAGCCCCC AGGGTTGGAG GACGTTGAGG CAGCGCTAGG CATGGACAGG 
CGCACGGATG CCTACAGCAG GTCGTCCTCA GTCTCCAGCC AGGAGGAGAA GTCGCTCCAC 
GCGGAGGAGC TGGTTGGCAG GGGCATCCCC ATCGAGCGAG TCGTCTCCTC GGAGGGTGGC 
CGGCCCTCTG TGGACCTCTC CTTCCAGCCC TCGCAGCCCC TGAGCAAGTC CAGCTCCTCT 
CCCGAGCTGC AGACTCTGCA GGACATCCTC GGGGACCCTG GGGACAAGGC CGACGTGGGC 
CGGCTGAGCC CTGAGGTTAA GGCCCGGTCA CAGTCAGGGA CCCTGGACGG GGAAAGTGCT 
GCCTGGTCGG CCTCGGGCGA AGACAGTCGG GGCCAGCCCG AGGGTCCCTT GCCTTCCAGC 
TCCCCCCGCT CGCCCAGTGG CCTCCGGCCC CGAGGTTACA CCATCTCCGA CTCGGCCCCA 
TCACGCAGGG GCAAGAGAGT AGAGAGGGAC GCCTTAAAGA GCAGAGCCAC AGCCTCCAAT 
GCAGAGAAAG TGCCAGGCAT CAACCCCAGT TTCGTGTTCC TGCAGCTCTA CCATTCCCCC 
TTCTTTGGCG ACGAGTCAAA CAAGCCAATC CTGCTGCCCA ATGAGTCACA GTCCTTTGAG 
CGGTCGGTGC AGCTCCTCGA CCAGATCCCA TCATACGACA CCCACAAGAT CGCCGTCCTG 
TATGTTGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAATGA GCATGGCTCC 
TACAGGTACA CGGAGTTCCT GACGGGCCTG GGCCGGCTCA TCGAGCTGAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG AGGCCTGGAC GTGTGTGGTG AGGACGGCCA GTTCACCTAC 
TGCTGGCACG ATGACATCAT GCAAGCCGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG CGACAAGAAG CGCCACCTGG GCAACGACTT TGTGTCCATT 
GTCTACAATG ACTCCGGTGA GGACTTCAAG CTTGGCACCA TCAAGGGCCA GTTCAACTTT 
GTCCACGTGA TCGTCACCCC GCTGGACTAC GAGTGCAACC TGGTGTCCCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGACACCAGC GTGGCCAAGA TCGTGTCTGA CCGCAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCAAATATGG CCTCACAGGT GCATCATAGC 
CGCTCCAACC CCACCGATAT CTACCCCTCC AAGTGGATTG CCCGGCTCCG CCACATCAAG 
CGGCTCCGCC AGCGGATCTG CGAGGAAGCC GCCTACTCCA ACCCCAGCCT ACCTCTGGTG 
CACCCTCCGT CCCATAGCAA AGCCCCTGCA CAGACTCCAG CCGAGCCCAC ACCTGGCTAT 
GAGGTGGGCC AGCGGAAGCG CCTCATCTCC TCGGTGGAGG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520940.2
CDS	19..5658
Translation	MPDSCGLHIPAAHAAPSRDARIAGLSARGRHRTTTPSKLPRRPSAQCRYRKCGSRFRRRPGARGVRLSPRRGGPERGGAAREGFSGASWSTMAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWAGIASQREAVGCRAPLCLIDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_011522638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522638.2

ORF Insert Sequence:

ATGCCGGACT CCTGCGGACT ACACATCCCG GCGGCCCATG CGGCCCCGTC ACGTGATGCA 
AGGATCGCCG GCCTTTCCGC CAGAGGGCGG CACAGAACTA CAACTCCCAG CAAGCTCCCA 
AGGCGGCCCT CCGCGCAATG CCGCTACCGG AAGTGCGGGT CGCGCTTCCG GCGGCGTCCC 
GGGGCCAGGG GGGTGCGCCT TTCTCCGCGT CGGGGCGGCC CGGAGCGCGG TGGCGCGGCG 
CGGGAGGGGT TTTCTGGTGC GTCCTGGTCC ACCATGGCCA AACCAACAAG CAAAGATTCA 
GGCTTGAAGG AGAAGTTTAA GATTCTGTTG GGACTGGGAA CACCGAGGCC AAATCCCAGG 
TCTGCAGAGG GTAAACAGAC GGAGTTTATC ATCACCGCGG AAATACTGAG AGAACTGAGC 
ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTCGCAAAA 
ACCAAGAAAT TTGAAGAGGG CGAGCGTTTG GGGGTCCTCA GAGCCCTCTT CTTTAAGGTC 
ATCAAGGATT ACCCTTCCAA CGAAGACCTT CACGAAAGGC TGGAGGTTTT CAAGGCCCTC 
ACAGACAATG GGAGACACAT CACCTACTTG GAGGAAGAGC TGGCTGACTT TGTCCTGCAG 
TGGATGGATG TTGGCTTGTC CTCGGAATTC CTTCTGGTGC TGGTGAACTT GGTCAAATTC 
AATAGCTGTT ACCTCGACGA GTACATCGCA AGGATGGTTC AGATGATCTG TCTGCTGTGC 
GTCCGGACCG CGTCCTCTGT GGACATAGAG GTCTCCCTGC AGGTGCTGGA CGCCGTGGTC 
TGCTACAACT GCCTGCCGGC TGAGAGCCTC CCGCTGTTCA TCGTTACCCT CTGTCGCACC 
ATCAACGTCA AGGAGCTCTG CGAGCCTTGC TGGAAGCTGA TGCGGAACCT CCTTGGCACC 
CACCTGGGCC ACAGCGCCAT CTACAACATG TGCCACCTCA TGGAGGACAG AGCCTACATG 
GAGGACGCGC CCCTGCTGAG AGGAGCCGTG TTTTTTGTGG GCATGGCTCT CTGGGGAGCC 
CACCGGCTCT ATTCTCTCAG GAACTCGCCG ACATCTGTGT TGCCATCATT TTACCAGGCC 
ATGGCATGTC CGAACGAGGT GGTGTCCTAT GAGATCGTCC TGTCCATCAC CAGGCTCATC 
AAGAAGTATA GGAAGGAGCT CCAGGTGGTG GCGTGGGACA TTCTGCTGAA CATCATCGAA 
CGGCTCCTTC AGCAGCTCCA GACCTTGGAC AGCCCGGAGC TCAGGACCAT CGTCCATGAC 
CTGTTGACCA CGGTGGAGGA GCTGTGTGAC CAGAACGAGT TCCACGGGTC TCAGGAGAGA 
TACTTTGAAC TGGTGGAGAG ATGTGCGGAC CAGAGGCCTG AGTCCTCCCT CCTGAACCTG 
ATCTCCTATA GAGCGCAGTC CATCCACCCG GCCAAGGACG GCTGGATTCA GAACCTGCAG 
GCGCTGATGG AGAGATTCTT CAGGAGCGAG TCCCGAGGCG CCGTGCGCAT CAAGGTGCTG 
GACGTGCTGT CCTTTGTGCT GCTCATCAAC AGGCAGTTCT ATGAGGAGGA GCTGATTAAC 
TCAGTGGTCA TCTCGCAGCT CTCCCACATC CCCGAGGATA AAGACCACCA GGTCCGAAAG 
CTGGCCACCC AGTTGCTGGT GGACCTGGCA GAGGGCTGCC ACACACACCA CTTCAACAGC 
CTGCTGGACA TCATCGAGAA GGTGATGGCC CGCTCCCTCT CCCCACCCCC GGAGCTGGAA 
GAAAGGGATG TGGCCGCATA CTCGGCCTCC TTGGAGGATG TGAAGACAGC CGTCCTGGGG 
CTTCTGGTCA TCCTTCAGAC CAAGCTGTAC ACCCTGCCTG CAAGCCACGC CACGCGTGTG 
TATGAGATGC TGGTCAGCCA CATTCAGCTC CACTACAAGC ACAGCTACAC CCTGCCAATC 
GCGAGCAGCA TCCGGCTGCA GGCCTTTGAC TTCCTGTTGC TGCTGCGGGC CGACTCACTG 
CACCGCCTGG GCCTGCCCAA CAAGGATGGA GTCGTGCGGT TCAGCCCCTA CTGCGTCTGC 
GACTACATGG AGCCAGAGAG AGGCTCTGAG AAGAAGACCA GCGGCCCCCT TTCTCCTCCC 
ACAGGGCCTC CTGGCCCGGC GCCTGCAGGC CCCGCCGTGC GGCTGGGGTC CGTGCCCTAC 
TCCCTGCTCT TCCGCGTCCT GCTGCAGTGC TTGAAGCAGG AGTCTGACTG GAAGGTGCTG 
AAGCTGGTTC TGGGCAGGCT GCCTGAGTCC CTGCGCTATA AAGTGCTCAT CTTTACTTCC 
CCTTGCAGTG TGGACCAGCT GTGCTCTGCT CTCTGCTCCA TGCTTTCAGG CCCAAAGACA 
CTGGAGCGGC TCCGAGGCGC CCCAGAAGGC TTCTCCAGAA CTGACTTGCA CCTGGCCGTG 
GTTCCAGTGC TGACAGCATT AATCTCTTAC CATAACTACC TGGACAAAAC CAAACAGCGC 
GAGATGGTCT ACTGCCTGGA GCAGGGCCTC ATCCACCGCT GTGCCAGCCA GTGCGTCGTG 
GCCTTGTCCA TCTGCAGCGT GGAGATGCCT GACATCATCA TCAAGGCGCT GCCTGTTCTG 
GTGGTGAAGC TCACGCACAT CTCAGCCACA GCCAGCATGG CCGTCCCACT GCTGGAGTTC 
CTGTCCACTC TGGCCAGGCT GCCGCACCTC TACAGGAACT TTGCCGCGGA GCAGTATGCC 
AGTGTGTTCG CCATCTCCCT GCCGTACACC AACCCCTCCA AGTTTAATCA GTACATCGTG 
TGTCTGGCCC ATCACGTCAT AGCCATGTGG TTCATCAGGT GCCGCCTGCC CTTCCGGAAG 
GATTTTGTCC CTTTCATCAC TAAGGGCCTG CGGTCCAATG TCCTCTTGTC TTTTGATGAC 
ACCCCCGAGA AGGACAGCTT CAGGGCCCGG AGTACTAGTC TCAACGAGAG ACCCAAGAGT 
CTGAGGATAG CCAGACCCCC CAAACAAGGC TTGAATAACT CTCCACCCGT GAAAGAATTC 
AAGGAGAGCT CTGCAGCCGA GGCCTTCCGG TGCCGCAGCA TCAGTGTGTC TGAACATGTG 
GTCCGCAGCA GGATACAGAC GTCCCTCACC AGTGCCAGCT TGGGGTCTGC AGATGAGAAC 
TCCGTGGCCC AGGCTGACGA TAGCCTGAAA AACCTCCACC TGGAGCTCAC GGAAACCTGT 
CTGGACATGA TGGCTCGATA CGTCTTCTCC AACTTCACGG CTGTCCCGAA GAGGTCTCCT 
GTGGGCGAGT TCCTCCTAGC GGGTGGCAGG ACCAAAACCT GGCTGGTTGG GAACAAGCTT 
GTCACTGTGA CGACAAGCGT GGGAACCGGG ACCCGGTCGT TACTAGGCCT GGACTCGGGG 
GAGCTGCAGT CCGGCCCGGA GTCGAGCTCC AGCCCCGGGG TGCATGTGAG ACAGACCAAG 
GAGGCGCCGG CCAAGCTGGA GTCCCAGGCT GGGCAGCAGG TGTCCCGTGG GGCCCGGGAT 
CGGGTCCGTT CCATGTCGGG GGGCCATGGT CTTCGAGTTG GCGCCCTGGA CGTGCCGGCC 
TCCCAGTTCC TGGGCAGTGC CACTTCTCCA GGACCACGGA CTGCACCAGC CGCGAAACCT 
GAGAAGGCCT CAGCTGGCAC CCGGGTTCCT GTGCAGGAGA AGACGAACCT GGCGGCCTAT 
GTGCCCCTGC TGACCCAGGG CTGGGCGGAG ATCCTGGTCC GGAGGCCCAC AGGGAACACC 
AGCTGGCTGA TGAGCCTGGA GAACCCGCTC AGCCCTTTCT CCTCGGACAT CAACAACATG 
CCCCTGCAGG AGCTGTCTAA CGCCCTCATG GCGGCTGAGC GCTTCAAGGA GCACCGGGAC 
ACAGCCCTGT ACAAGTCACT GTCGGTGCCG GCAGCCAGCA CGGCCAAACC CCCTCCTCTG 
CCTCGCTCCA ACACAGTGGC CTCTTTCTCC TCCCTGTACC AGTCCAGCTG CCAAGGACAG 
CTGCACAGGA GCGTTTCCTG GGCAGGTATC GCCTCTCAGA GGGAAGCGGT TGGCTGCAGA 
GCGCCACTCT GCCTCATAGA CTCCGCCGTG GTCATGGAGG AGGGAAGTCC GGGCGAGGTT 
CCTGTGCTGG TGGAGCCCCC AGGGTTGGAG GACGTTGAGG CAGCGCTAGG CATGGACAGG 
CGCACGGATG CCTACAGCAG GTCGTCCTCA GTCTCCAGCC AGGAGGAGAA GTCGCTCCAC 
GCGGAGGAGC TGGTTGGCAG GGGCATCCCC ATCGAGCGAG TCGTCTCCTC GGAGGGTGGC 
CGGCCCTCTG TGGACCTCTC CTTCCAGCCC TCGCAGCCCC TGAGCAAGTC CAGCTCCTCT 
CCCGAGCTGC AGACTCTGCA GGACATCCTC GGGGACCCTG GGGACAAGGC CGACGTGGGC 
CGGCTGAGCC CTGAGGTTAA GGCCCGGTCA CAGTCAGGGA CCCTGGACGG GGAAAGTGCT 
GCCTGGTCGG CCTCGGGCGA AGACAGTCGG GGCCAGCCCG AGGGTCCCTT GCCTTCCAGC 
TCCCCCCGCT CGCCCAGTGG CCTCCGGCCC CGAGGTTACA CCATCTCCGA CTCGGCCCCA 
TCACGCAGGG GCAAGAGAGT AGAGAGGGAC GCCTTAAAGA GCAGAGCCAC AGCCTCCAAT 
GCAGAGAAAG TGCCAGGCAT CAACCCCAGT TTCGTGTTCC TGCAGCTCTA CCATTCCCCC 
TTCTTTGGCG ACGAGTCAAA CAAGCCAATC CTGCTGCCCA ATGAGTCACA GTCCTTTGAG 
CGGTCGGTGC AGCTCCTCGA CCAGATCCCA TCATACGACA CCCACAAGAT CGCCGTCCTG 
TATGTTGGAG AAGGCCAGAG CAACAGCGAG CTCGCCATCC TGTCCAATGA GCATGGCTCC 
TACAGGTACA CGGAGTTCCT GACGGGCCTG GGCCGGCTCA TCGAGCTGAA GGACTGCCAG 
CCGGACAAGG TGTACCTGGG AGGCCTGGAC GTGTGTGGTG AGGACGGCCA GTTCACCTAC 
TGCTGGCACG ATGACATCAT GCAAGCCGTC TTCCACATCG CCACCCTGAT GCCCACCAAG 
GACGTGGACA AGCACCGCTG CGACAAGAAG CGCCACCTGG GCAACGACTT TGTGTCCATT 
GTCTACAATG ACTCCGGTGA GGACTTCAAG CTTGGCACCA TCAAGGGCCA GTTCAACTTT 
GTCCACGTGA TCGTCACCCC GCTGGACTAC GAGTGCAACC TGGTGTCCCT GCAGTGCAGG 
AAAGACATGG AGGGCCTTGT GGACACCAGC GTGGCCAAGA TCGTGTCTGA CCGCAACCTG 
CCCTTCGTGG CCCGCCAGAT GGCCCTGCAC GCAAATATGG CCTCACAGGT GCATCATAGC 
CGCTCCAACC CCACCGATAT CTACCCCTCC AAGTGGATTG CCCGGCTCCG CCACATCAAG 
CGGCTCCGCC AGCGGATCTG CGAGGAAGCC GCCTACTCCA ACCCCAGCCT ACCTCTGGTG 
CACCCTCCGT CCCATAGCAA AGCCCCTGCA CAGACTCCAG CCGAGCCCAC ACCTGGCTAT 
GAGGTGGGCC AGCGGAAGCG CCTCATCTCC TCGGTGGAGG ACTTCACCGA GTTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_017023617.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879106.1 Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

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CloneID	OHu86242
Clone ID Related Accession (Same CDS sequence)	XM_017023617.1
Accession Version	XM_017023617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tuberin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACT CCTGCGGACT ACACATCCCG GCGGCCCATG CGGCCCCGTC ACGTGATGCA 
AGGATCGCCG GCCTTTCCGC CAGAGGGCGG CACAGAACTA CAACTCCCAG CAAGCTCCCA 
AGGCGGCCCT CCGCGCAATG CCGCTACCGG AAGTGCGGGT CGCGCTTCCG GCGGCGTCCC 
GGGGCCAGGG GGGTGCGCCT TTCTCCGCGT CGGGGCGGCC CGGAGCGCGG TGGCGCGGCG 
CGGGAGGGGT TTTCTGGTGC GTCCTGGTCC ACCATGGCCA AACCAACAAG CAAAGATTCA 
GGCTTGAAGG AGAAGTTTAA GATTCTGTTG GGACTGGGAA CACCGAGGCC AAATCCCAGG 
TCTGCAGAGG GTAAACAGAC GGAGTTTATC ATCACCGCGG AAATACTGAG AGAACTGAGC 
ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTCGCAAAA 
ACCAAGAAAT TTGAAGAGGG CGAGCGTTTG GGGGTCCTCA GAGCCCTCTT CTTTAAGGTC 
ATCAAGGATT ACCCTTCCAA CGAAGACCTT CACGAAAGGC TGGAGGTTTT CAAGGCCCTC 
ACAGACAATG GGAGACACAT CACCTACTTG GAGGAAGAGC TGGCTGACTT TGTCCTGCAG 
TGGATGGATG TTGGCTTGTC CTCGGAATTC CTTCTGGTGC TGGTGAACTT GGTCAAATTC 
AATAGCTGTT ACCTCGACGA GTACATCGCA AGGATGGTTC AGATGATCTG TCTGCTGTGC 
GTCCGGACCG CGTCCTCTGT GGACATAGAG GTCTCCCTGC AGGTGCTGGA CGCCGTGGTC 
TGCTACAACT GCCTGCCGGC TGAGAGCCTC CCGCTGTTCA TCGTTACCCT CTGTCGCACC 
ATCAACGTCA AGGAGCTCTG CGAGCCTTGC TGGAAGCTGA TGCGGAACCT CCTTGGCACC 
CACCTGGGCC ACAGCGCCAT CTACAACATG TGCCACCTCA TGGAGGACAG AGCCTACATG 
GAGGACGCGC CCCTGCTGAG AGGAGCCGTG TTTTTTGTGG GCATGGCTCT CTGGGGAGCC 
CACCGGCTCT ATTCTCTCAG GAACTCGCCG ACATCTGTGT TGCCATCATT TTACCAGGCC 
ATGGCATGTC CGAACGAGGT GGTGTCCTAT GAGATCGTCC TGTCCATCAC CAGGCTCATC 
AAGAAGTATA GGAAGGAGCT CCAGGTGGTG GCGTGGGACA TTCTGCTGAA CATCATCGAA 
CGGCTCCTTC AGCAGCTCCA GACCTTGGAC AGCCCGGAGC TCAGGACCAT CGTCCATGAC 
CTGTTGACCA CGGTGGAGGA GCTGTGTGAC CAGAACGAGT TCCACGGGTC TCAGGAGAGA 
TACTTTGAAC TGGTGGAGAG ATGTGCGGAC CAGAGGCCTG AGTCCTCCCT CCTGAACCTG 
ATCTCCTATA GAGCGCAGTC CATCCACCCG GCCAAGGACG GCTGGATTCA GAACCTGCAG 
GCGCTGATGG AGAGATTCTT CAGGAGCGAG TCCCGAGGCG CCGTGCGCAT CAAGGTGCTG 
GACGTGCTGT CCTTTGTGCT GCTCATCAAC AGGCAGTTCT ATGAGGAGGA GCTGATTAAC 
TCAGTGGTCA TCTCGCAGCT CTCCCACATC CCCGAGGATA AAGACCACCA GGTCCGAAAG 
CTGGCCACCC AGTTGCTGGT GGACCTGGCA GAGGGCTGCC ACACACACCA CTTCAACAGC 
CTGCTGGACA TCATCGAGAA GGTGATGGCC CGCTCCCTCT CCCCACCCCC GGAGCTGGAA 
GAAAGGGATG TGGCCGCATA CTCGGCCTCC TTGGAGGATG TGAAGACAGC CGTCCTGGGG 
CTTCTGGTCA TCCTTCAGAC CAAGCTGTAC ACCCTGCCTG CAAGCCACGC CACGCGTGTG 
TATGAGATGC TGGTCAGCCA CATTCAGCTC CACTACAAGC ACAGCTACAC CCTGCCAATC 
GCGAGCAGCA TCCGGCTGCA GGCCTTTGAC TTCCTGTTGC TGCTGCGGGC CGACTCACTG 
CACCGCCTGG GCCTGCCCAA CAAGGATGGA GTCGTGCGGT TCAGCCCCTA CTGCGTCTGC 
GACTACATGG AGCCAGAGAG AGGCTCTGAG AAGAAGACCA GCGGCCCCCT TTCTCCTCCC 
ACAGGGCCTC CTGGCCCGGC GCCTGCAGGC CCCGCCGTGC GGCTGGGGTC CGTGCCCTAC 
TCCCTGCTCT TCCGCGTCCT GCTGCAGTGC TTGAAGCAGG AGTCTGACTG GAAGGTGCTG 
AAGCTGGTTC TGGGCAGGCT GCCTGAGTCC CTGCGCTATA AAGTGCTCAT CTTTACTTCC 
CCTTGCAGTG TGGACCAGCT GTGCTCTGCT CTCTGCTCCA TGCTTTCAGG CCCAAAGACA 
CTGGAGCGGC TCCGAGGCGC CCCAGAAGGC TTCTCCAGAA CTGACTTGCA CCTGGCCGTG 
GTTCCAGTGC TGACAGCATT AATCTCTTAC CATAACTACC TGGACAAAAC CAAACAGCGC 
GAGATGGTCT ACTGCCTGGA GCAGGGCCTC ATCCACCGCT GTGCCAGCCA GTGCGTCGTG 
GCCTTGTCCA TCTGCAGCGT GGAGATGCCT GACATCATCA TCAAGGCGCT GCCTGTTCTG 
GTGGTGAAGC TCACGCACAT CTCAGCCACA GCCAGCATGG CCGTCCCACT GCTGGAGTTC 
CTGTCCACTC TGGCCAGGCT GCCGCACCTC TACAGGAACT TTGCCGCGGA GCAGTATGCC 
AGTGTGTTCG CCATCTCCCT GCCGTACACC AACCCCTCCA AGTTTAATCA GTACATCGTG 
TGTCTGGCCC ATCACGTCAT AGCCATGTGG TTCATCAGGT GCCGCCTGCC CTTCCGGAAG 
GATTTTGTCC CTTTCATCAC TAAGGGCCTG CGGTCCAATG TCCTCTTGTC TTTTGATGAC 
ACCCCCGAGA AGGACAGCTT CAGGGCCCGG AGTACTAGTC TCAACGAGAG ACCCAAGAGC 
AGGATACAGA CGTCCCTCAC CAGTGCCAGC TTGGGGTCTG CAGATGAGAA CTCCGTGGCC 
CAGGCTGACG ATAGCCTGAA AAACCTCCAC CTGGAGCTCA CGGAAACCTG TCTGGACATG 
ATGGCTCGAT ACGTCTTCTC CAACTTCACG GCTGTCCCGA AGAGGTCTCC TGTGGGCGAG 
TTCCTCCTAG CGGGTGGCAG GACCAAAACC TGGCTGGTTG GGAACAAGCT TGTCACTGTG 
ACGACAAGCG TGGGAACCGG GACCCGGTCG TTACTAGGCC TGGACTCGGG GGAGCTGCAG 
TCCGGCCCGG AGTCGAGCTC CAGCCCCGGG GTGCATGTGA GACAGACCAA GGAGGCGCCG 
GCCAAGCTGG AGTCCCAGGC TGGGCAGCAG GTGTCCCGTG GGGCCCGGGA TCGGGTCCGT 
TCCATGTCGG GGGGCCATGG TCTTCGAGTT GGCGCCCTGG ACGTGCCGGC CTCCCAGTTC 
CTGGGCAGTG CCACTTCTCC AGGACCACGG ACTGCACCAG CCGCGAAACC TGAGAAGGCC 
TCAGCTGGCA CCCGGGTTCC TGTGCAGGAG AAGACGAACC TGGCGGCCTA TGTGCCCCTG 
CTGACCCAGG GCTGGGCGGA GATCCTGGTC CGGAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCTTTC TCCTCGGACA TCAACAACAT GCCCCTGCAG 
GAGCTGTCTA ACGCCCTCAT GGCGGCTGAG CGCTTCAAGG AGCACCGGGA CACAGCCCTG 
TACAAGTCAC TGTCGGTGCC GGCAGCCAGC ACGGCCAAAC CCCCTCCTCT GCCTCGCTCC 
AACACAGACT CCGCCGTGGT CATGGAGGAG GGAAGTCCGG GCGAGGTTCC TGTGCTGGTG 
GAGCCCCCAG GGTTGGAGGA CGTTGAGGCA GCGCTAGGCA TGGACAGGCG CACGGATGCC 
TACAGCAGGT CGTCCTCAGT CTCCAGCCAG GAGGAGAAGT CGCTCCACGC GGAGGAGCTG 
GTTGGCAGGG GCATCCCCAT CGAGCGAGTC GTCTCCTCGG AGGGTGGCCG GCCCTCTGTG 
GACCTCTCCT TCCAGCCCTC GCAGCCCCTG AGCAAGTCCA GCTCCTCTCC CGAGCTGCAG 
ACTCTGCAGG ACATCCTCGG GGACCCTGGG GACAAGGCCG ACGTGGGCCG GCTGAGCCCT 
GAGGTTAAGG CCCGGTCACA GTCAGGGACC CTGGACGGGG AAAGTGCTGC CTGGTCGGCC 
TCGGGCGAAG ACAGTCGGGG CCAGCCCGAG GGTCCCTTGC CTTCCAGCTC CCCCCGCTCG 
CCCAGTGGCC TCCGGCCCCG AGGTTACACC ATCTCCGACT CGGCCCCATC ACGCAGGGGC 
AAGAGAGTAG AGAGGGACGC CTTAAAGAGC AGAGCCACAG CCTCCAATGC AGAGAAAGTG 
CCAGGCATCA ACCCCAGTTT CGTGTTCCTG CAGCTCTACC ATTCCCCCTT CTTTGGCGAC 
GAGTCAAACA AGCCAATCCT GCTGCCCAAT GAGTCACAGT CCTTTGAGCG GTCGGTGCAG 
CTCCTCGACC AGATCCCATC ATACGACACC CACAAGATCG CCGTCCTGTA TGTTGGAGAA 
GGCCAGAGCA ACAGCGAGCT CGCCATCCTG TCCAATGAGC ATGGCTCCTA CAGGTACACG 
GAGTTCCTGA CGGGCCTGGG CCGGCTCATC GAGCTGAAGG ACTGCCAGCC GGACAAGGTG 
TACCTGGGAG GCCTGGACGT GTGTGGTGAG GACGGCCAGT TCACCTACTG CTGGCACGAT 
GACATCATGC AAGCCGTCTT CCACATCGCC ACCCTGATGC CCACCAAGGA CGTGGACAAG 
CACCGCTGCG ACAAGAAGCG CCACCTGGGC AACGACTTTG TGTCCATTGT CTACAATGAC 
TCCGGTGAGG ACTTCAAGCT TGGCACCATC AAGGGCCAGT TCAACTTTGT CCACGTGATC 
GTCACCCCGC TGGACTACGA GTGCAACCTG GTGTCCCTGC AGTGCAGGAA AGACATGGAG 
GGCCTTGTGG ACACCAGCGT GGCCAAGATC GTGTCTGACC GCAACCTGCC CTTCGTGGCC 
CGCCAGATGG CCCTGCACGC AAATATGGCC TCACAGGTGC ATCATAGCCG CTCCAACCCC 
ACCGATATCT ACCCCTCCAA GTGGATTGCC CGGCTCCGCC ACATCAAGCG GCTCCGCCAG 
CGGATCTGCG AGGAAGCCGC CTACTCCAAC CCCAGCCTAC CTCTGGTGCA CCCTCCGTCC 
CATAGCAAAG CCCCTGCACA GACTCCAGCC GAGCCCACAC CTGGCTATGA GGTGGGCCAG 
CGGAAGCGCC TCATCTCCTC GGTGGAGGAC TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879106.1
CDS	19..5406
Translation	MPDSCGLHIPAAHAAPSRDARIAGLSARGRHRTTTPSKLPRRPSAQCRYRKCGSRFRRRPGARGVRLSPRRGGPERGGAAREGFSGASWSTMAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Target ORF information:

RefSeq Version	XM_017023617.1
Organism	Homo sapiens(human)
Definition	Homo sapiens TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023617.1

ORF Insert Sequence:

ATGCCGGACT CCTGCGGACT ACACATCCCG GCGGCCCATG CGGCCCCGTC ACGTGATGCA 
AGGATCGCCG GCCTTTCCGC CAGAGGGCGG CACAGAACTA CAACTCCCAG CAAGCTCCCA 
AGGCGGCCCT CCGCGCAATG CCGCTACCGG AAGTGCGGGT CGCGCTTCCG GCGGCGTCCC 
GGGGCCAGGG GGGTGCGCCT TTCTCCGCGT CGGGGCGGCC CGGAGCGCGG TGGCGCGGCG 
CGGGAGGGGT TTTCTGGTGC GTCCTGGTCC ACCATGGCCA AACCAACAAG CAAAGATTCA 
GGCTTGAAGG AGAAGTTTAA GATTCTGTTG GGACTGGGAA CACCGAGGCC AAATCCCAGG 
TCTGCAGAGG GTAAACAGAC GGAGTTTATC ATCACCGCGG AAATACTGAG AGAACTGAGC 
ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTCGCAAAA 
ACCAAGAAAT TTGAAGAGGG CGAGCGTTTG GGGGTCCTCA GAGCCCTCTT CTTTAAGGTC 
ATCAAGGATT ACCCTTCCAA CGAAGACCTT CACGAAAGGC TGGAGGTTTT CAAGGCCCTC 
ACAGACAATG GGAGACACAT CACCTACTTG GAGGAAGAGC TGGCTGACTT TGTCCTGCAG 
TGGATGGATG TTGGCTTGTC CTCGGAATTC CTTCTGGTGC TGGTGAACTT GGTCAAATTC 
AATAGCTGTT ACCTCGACGA GTACATCGCA AGGATGGTTC AGATGATCTG TCTGCTGTGC 
GTCCGGACCG CGTCCTCTGT GGACATAGAG GTCTCCCTGC AGGTGCTGGA CGCCGTGGTC 
TGCTACAACT GCCTGCCGGC TGAGAGCCTC CCGCTGTTCA TCGTTACCCT CTGTCGCACC 
ATCAACGTCA AGGAGCTCTG CGAGCCTTGC TGGAAGCTGA TGCGGAACCT CCTTGGCACC 
CACCTGGGCC ACAGCGCCAT CTACAACATG TGCCACCTCA TGGAGGACAG AGCCTACATG 
GAGGACGCGC CCCTGCTGAG AGGAGCCGTG TTTTTTGTGG GCATGGCTCT CTGGGGAGCC 
CACCGGCTCT ATTCTCTCAG GAACTCGCCG ACATCTGTGT TGCCATCATT TTACCAGGCC 
ATGGCATGTC CGAACGAGGT GGTGTCCTAT GAGATCGTCC TGTCCATCAC CAGGCTCATC 
AAGAAGTATA GGAAGGAGCT CCAGGTGGTG GCGTGGGACA TTCTGCTGAA CATCATCGAA 
CGGCTCCTTC AGCAGCTCCA GACCTTGGAC AGCCCGGAGC TCAGGACCAT CGTCCATGAC 
CTGTTGACCA CGGTGGAGGA GCTGTGTGAC CAGAACGAGT TCCACGGGTC TCAGGAGAGA 
TACTTTGAAC TGGTGGAGAG ATGTGCGGAC CAGAGGCCTG AGTCCTCCCT CCTGAACCTG 
ATCTCCTATA GAGCGCAGTC CATCCACCCG GCCAAGGACG GCTGGATTCA GAACCTGCAG 
GCGCTGATGG AGAGATTCTT CAGGAGCGAG TCCCGAGGCG CCGTGCGCAT CAAGGTGCTG 
GACGTGCTGT CCTTTGTGCT GCTCATCAAC AGGCAGTTCT ATGAGGAGGA GCTGATTAAC 
TCAGTGGTCA TCTCGCAGCT CTCCCACATC CCCGAGGATA AAGACCACCA GGTCCGAAAG 
CTGGCCACCC AGTTGCTGGT GGACCTGGCA GAGGGCTGCC ACACACACCA CTTCAACAGC 
CTGCTGGACA TCATCGAGAA GGTGATGGCC CGCTCCCTCT CCCCACCCCC GGAGCTGGAA 
GAAAGGGATG TGGCCGCATA CTCGGCCTCC TTGGAGGATG TGAAGACAGC CGTCCTGGGG 
CTTCTGGTCA TCCTTCAGAC CAAGCTGTAC ACCCTGCCTG CAAGCCACGC CACGCGTGTG 
TATGAGATGC TGGTCAGCCA CATTCAGCTC CACTACAAGC ACAGCTACAC CCTGCCAATC 
GCGAGCAGCA TCCGGCTGCA GGCCTTTGAC TTCCTGTTGC TGCTGCGGGC CGACTCACTG 
CACCGCCTGG GCCTGCCCAA CAAGGATGGA GTCGTGCGGT TCAGCCCCTA CTGCGTCTGC 
GACTACATGG AGCCAGAGAG AGGCTCTGAG AAGAAGACCA GCGGCCCCCT TTCTCCTCCC 
ACAGGGCCTC CTGGCCCGGC GCCTGCAGGC CCCGCCGTGC GGCTGGGGTC CGTGCCCTAC 
TCCCTGCTCT TCCGCGTCCT GCTGCAGTGC TTGAAGCAGG AGTCTGACTG GAAGGTGCTG 
AAGCTGGTTC TGGGCAGGCT GCCTGAGTCC CTGCGCTATA AAGTGCTCAT CTTTACTTCC 
CCTTGCAGTG TGGACCAGCT GTGCTCTGCT CTCTGCTCCA TGCTTTCAGG CCCAAAGACA 
CTGGAGCGGC TCCGAGGCGC CCCAGAAGGC TTCTCCAGAA CTGACTTGCA CCTGGCCGTG 
GTTCCAGTGC TGACAGCATT AATCTCTTAC CATAACTACC TGGACAAAAC CAAACAGCGC 
GAGATGGTCT ACTGCCTGGA GCAGGGCCTC ATCCACCGCT GTGCCAGCCA GTGCGTCGTG 
GCCTTGTCCA TCTGCAGCGT GGAGATGCCT GACATCATCA TCAAGGCGCT GCCTGTTCTG 
GTGGTGAAGC TCACGCACAT CTCAGCCACA GCCAGCATGG CCGTCCCACT GCTGGAGTTC 
CTGTCCACTC TGGCCAGGCT GCCGCACCTC TACAGGAACT TTGCCGCGGA GCAGTATGCC 
AGTGTGTTCG CCATCTCCCT GCCGTACACC AACCCCTCCA AGTTTAATCA GTACATCGTG 
TGTCTGGCCC ATCACGTCAT AGCCATGTGG TTCATCAGGT GCCGCCTGCC CTTCCGGAAG 
GATTTTGTCC CTTTCATCAC TAAGGGCCTG CGGTCCAATG TCCTCTTGTC TTTTGATGAC 
ACCCCCGAGA AGGACAGCTT CAGGGCCCGG AGTACTAGTC TCAACGAGAG ACCCAAGAGC 
AGGATACAGA CGTCCCTCAC CAGTGCCAGC TTGGGGTCTG CAGATGAGAA CTCCGTGGCC 
CAGGCTGACG ATAGCCTGAA AAACCTCCAC CTGGAGCTCA CGGAAACCTG TCTGGACATG 
ATGGCTCGAT ACGTCTTCTC CAACTTCACG GCTGTCCCGA AGAGGTCTCC TGTGGGCGAG 
TTCCTCCTAG CGGGTGGCAG GACCAAAACC TGGCTGGTTG GGAACAAGCT TGTCACTGTG 
ACGACAAGCG TGGGAACCGG GACCCGGTCG TTACTAGGCC TGGACTCGGG GGAGCTGCAG 
TCCGGCCCGG AGTCGAGCTC CAGCCCCGGG GTGCATGTGA GACAGACCAA GGAGGCGCCG 
GCCAAGCTGG AGTCCCAGGC TGGGCAGCAG GTGTCCCGTG GGGCCCGGGA TCGGGTCCGT 
TCCATGTCGG GGGGCCATGG TCTTCGAGTT GGCGCCCTGG ACGTGCCGGC CTCCCAGTTC 
CTGGGCAGTG CCACTTCTCC AGGACCACGG ACTGCACCAG CCGCGAAACC TGAGAAGGCC 
TCAGCTGGCA CCCGGGTTCC TGTGCAGGAG AAGACGAACC TGGCGGCCTA TGTGCCCCTG 
CTGACCCAGG GCTGGGCGGA GATCCTGGTC CGGAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCTTTC TCCTCGGACA TCAACAACAT GCCCCTGCAG 
GAGCTGTCTA ACGCCCTCAT GGCGGCTGAG CGCTTCAAGG AGCACCGGGA CACAGCCCTG 
TACAAGTCAC TGTCGGTGCC GGCAGCCAGC ACGGCCAAAC CCCCTCCTCT GCCTCGCTCC 
AACACAGACT CCGCCGTGGT CATGGAGGAG GGAAGTCCGG GCGAGGTTCC TGTGCTGGTG 
GAGCCCCCAG GGTTGGAGGA CGTTGAGGCA GCGCTAGGCA TGGACAGGCG CACGGATGCC 
TACAGCAGGT CGTCCTCAGT CTCCAGCCAG GAGGAGAAGT CGCTCCACGC GGAGGAGCTG 
GTTGGCAGGG GCATCCCCAT CGAGCGAGTC GTCTCCTCGG AGGGTGGCCG GCCCTCTGTG 
GACCTCTCCT TCCAGCCCTC GCAGCCCCTG AGCAAGTCCA GCTCCTCTCC CGAGCTGCAG 
ACTCTGCAGG ACATCCTCGG GGACCCTGGG GACAAGGCCG ACGTGGGCCG GCTGAGCCCT 
GAGGTTAAGG CCCGGTCACA GTCAGGGACC CTGGACGGGG AAAGTGCTGC CTGGTCGGCC 
TCGGGCGAAG ACAGTCGGGG CCAGCCCGAG GGTCCCTTGC CTTCCAGCTC CCCCCGCTCG 
CCCAGTGGCC TCCGGCCCCG AGGTTACACC ATCTCCGACT CGGCCCCATC ACGCAGGGGC 
AAGAGAGTAG AGAGGGACGC CTTAAAGAGC AGAGCCACAG CCTCCAATGC AGAGAAAGTG 
CCAGGCATCA ACCCCAGTTT CGTGTTCCTG CAGCTCTACC ATTCCCCCTT CTTTGGCGAC 
GAGTCAAACA AGCCAATCCT GCTGCCCAAT GAGTCACAGT CCTTTGAGCG GTCGGTGCAG 
CTCCTCGACC AGATCCCATC ATACGACACC CACAAGATCG CCGTCCTGTA TGTTGGAGAA 
GGCCAGAGCA ACAGCGAGCT CGCCATCCTG TCCAATGAGC ATGGCTCCTA CAGGTACACG 
GAGTTCCTGA CGGGCCTGGG CCGGCTCATC GAGCTGAAGG ACTGCCAGCC GGACAAGGTG 
TACCTGGGAG GCCTGGACGT GTGTGGTGAG GACGGCCAGT TCACCTACTG CTGGCACGAT 
GACATCATGC AAGCCGTCTT CCACATCGCC ACCCTGATGC CCACCAAGGA CGTGGACAAG 
CACCGCTGCG ACAAGAAGCG CCACCTGGGC AACGACTTTG TGTCCATTGT CTACAATGAC 
TCCGGTGAGG ACTTCAAGCT TGGCACCATC AAGGGCCAGT TCAACTTTGT CCACGTGATC 
GTCACCCCGC TGGACTACGA GTGCAACCTG GTGTCCCTGC AGTGCAGGAA AGACATGGAG 
GGCCTTGTGG ACACCAGCGT GGCCAAGATC GTGTCTGACC GCAACCTGCC CTTCGTGGCC 
CGCCAGATGG CCCTGCACGC AAATATGGCC TCACAGGTGC ATCATAGCCG CTCCAACCCC 
ACCGATATCT ACCCCTCCAA GTGGATTGCC CGGCTCCGCC ACATCAAGCG GCTCCGCCAG 
CGGATCTGCG AGGAAGCCGC CTACTCCAAC CCCAGCCTAC CTCTGGTGCA CCCTCCGTCC 
CATAGCAAAG CCCCTGCACA GACTCCAGCC GAGCCCACAC CTGGCTATGA GGTGGGCCAG 
CGGAAGCGCC TCATCTCCTC GGTGGAGGAC TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC2
Entrez Gene ID	7249
Full Name	TSC complex subunit 2
Synonyms	LAM,PPP1R160,TSC4
General protein information	Preferred Names TSC complex subunit 2 Names tuberin protein phosphatase 1, regulatory subunit 160 tuberous sclerosis 2 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:	191092
Disorder Html:	Tuberous sclerosis-2, 613254 (3); Lymphangioleiomyomatosis, somatic, 606690 (3); ?Focal cortical dysplasia, type II, somatic, 607341 (3)

Pan troglodytes (chimpanzee)	TSC2	XP_523477.3
Mus musculus (house mouse)	Tsc2	NP_001034452.1
Rattus norvegicus (Norway rat)	Tsc2	NP_036812.2
Gallus gallus (chicken)	TSC2	XP_003642168.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011123	XP_309523.4
Canis lupus familiaris (dog)	TSC2	XP_852047.2
Bos taurus (cattle)	TSC2	XP_003587856.2
Drosophila melanogaster (fruit fly)	gig	NP_524177.1
Homo sapiens (human)	TSC2	NP_000539.2
	TSC2	XP_001083256.1
Danio rerio (zebrafish)	tsc2	XP_695912.6
Xenopus tropicalis (tropical clawed frog)	tsc2	NP_001123795.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

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