gig cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gig
Entrez Gene ID	40201
Full Name	gigas
Synonyms	6975,C1,CG6975,Dmel\CG6975,FBpp0074588,Gigas,ME 109,TSC2,Tsc2,Tsc2/gig,dTSC2,dTsc2,gig/Tsc2,gig,Tsc2,l(3)109,tsc2
General protein information	Preferred Names gigas Names CG6975 gene product from transcript CG6975-RA CG6975-PA CG6975-PB gig-PA gig-PB lethal(3)109 tuberous sclerosis complex 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76F2-76F2\|3-46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079453.3	NP_524177.1	gigas, isoform A
NM_001275160.1	NP_001262089.1	gigas, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSC2	XP_523477.3
Mus musculus (house mouse)	Tsc2	NP_001034452.1
Rattus norvegicus (Norway rat)	Tsc2	NP_036812.2
Gallus gallus (chicken)	TSC2	XP_003642168.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011123	XP_309523.4
Canis lupus familiaris (dog)	TSC2	XP_852047.2
Bos taurus (cattle)	TSC2	XP_003587856.2
Drosophila melanogaster (fruit fly)	gig	NP_524177.1
Homo sapiens (human)	TSC2	NP_000539.2
	TSC2	XP_001083256.1
Danio rerio (zebrafish)	tsc2	XP_695912.6
Xenopus tropicalis (tropical clawed frog)	tsc2	NP_001123795.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data identify the eIF4F complex as an important upstream regulator of TORC1, which acts via TSC2 to inactivate TORC1 upon withdrawal of amino acids
Title: eIF4A inactivates TORC1 in response to amino acid?starvation.

Results identified Ser1338 in Drosophila TSC2 as the site for AMPK phosphorylation. Its mutation diminished the function of TSC2 in suppressing mTORC1-dependent cell growth in Drosophila wing epithelium.
Title: Identification of an AMPK phosphorylation site in Drosophila TSC2 (gigas) that regulate cell growth.

Data indicate that TORC1 activation by PVR involves Tsc1/Tsc2.
Title: Platelet-derived growth factor/vascular endothelial growth factor receptor inactivation by sunitinib results in Tsc1/Tsc2-dependent inhibition of TORC1.

hyperactivation of TORC1 following the loss of TSC1/2 is detrimental to stem cell maintenance and multiple lineage differentiation in the Drosophila intestinal stem cell lineage, a mechanism that could be conserved in other stem cell lineages
Title: TSC1/2 regulates intestinal stem cell maintenance and lineage differentiation through Rheb-TORC1-S6K but independently of nutritional status or Notch regulation.

deregulation of TORC1 induces activation of JNK, indicate that multiple cellular stresses are induced and contribute to the synthetic-lethal interactions between RB and TSC1/TSC2 inactivation.
Title: Deregulated G1-S control and energy stress contribute to the synthetic-lethal interactions between inactivation of RB and TSC1 or TSC2.

The following gig gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gig cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19059	NM_079453.3 Latest version!	Drosophila melanogaster gigas (gig), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa21111	NM_001275160.1 Latest version!	Drosophila melanogaster gigas (gig), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gig ( NM_079453.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524177.1 Drosophila melanogaster gigas (gig), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19059
Clone ID Related Accession (Same CDS sequence)	NM_079453.3
Accession Version	NM_079453.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gigas, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079453.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCA AGGACAAGTC CAAGTTCAAG TTGTTCCTCA AATCGCTGCC GGCAGGTTAC 
GTGGGCGAGC GGACCCTGCG GCCGGAGTTT GAGAGGGAAC TGCGTCCGGA GCAGCCGGTG 
GCCCAGCGCT GCCGGATGCT GAAAGAGCTG GGCGACACGC AGCTGCACAA CTTCAATCTG 
GACGAGTCCG CCATCACCAT TCTGTTCAAT CTCACCAACG ATCTCATCGT GCCCAACAAG 
CCGGCAGAAA CGCGGCAGAT TGCCTTGAGC TTCTATAAAC GGCTCATTCA CACACAGTAC 
AAGAACCTGA CCATCATGCG GGAGAAGTTC TTCCTGGTCA TCCAGAACCA CGAAGCCCGC 
GAAGATCTGC GTCACCTTCT TGAGCTGCTC GACACCCTCA CCGACAATGG GAAGGATATC 
ACAAACTTCG AGGAGAAGAT TGGCAAGTTT ATGCTGCTCT GGATCCCAGC CATTACGGAG 
GCCAATCTGC TTACCCCCTA CCTTGACATT CTGGTCAACC TGATCAAGTT CAATGCGGCG 
CATCTGGACA AAGACATCCT TGTGGGCATC GTGCAGAACG CTTGCGATCT GAGCTGTAGC 
GTTACCGTAA ACGAAATCGG TCTGCAGTGC CTCACAATTC TGGAGATGGT CATCGGGTAC 
ACCATCTTCC CAAGCGAACC GCTGCCGCAG TGCATCACCA CCTTGTGCCG GACTGTGAAT 
CATGCTCCTT ACTGCCCGTC TTCTTTCAAG ATCATGAAGA ACCTCTTGGG CACCCAGTTG 
GGATACCATT CCATGAAGAT GATGTGCAGT ATCCTCAACG ATCGAGCTCT TTACGATGAT 
GCCCACCTGT TGCGTGGGGC CGTGTTCCAT CTAAACATGA ACATCTTTGG CTCGAACATT 
ATTTTCCAGG TTTCGCCTAT GACCTATGCA ACGAATGTGC TGACAGCCTT CCTGCGGGCA 
CTAGACAGTC GTCAGGTGAT CGTGACCTTC GAAGTCATCC TCAGCGTTCG CATGGTGATT 
ACCAAGCGGC AGCTTTCAGA GATCATTTGG GATCTGATCT GCGATATAAT GTCCTCGATC 
GTTAGCAATA TCGAGTATTA CGAAGAAGTC AATATAAACA AGGATCGTCT GCATCACCTG 
CAGATCAATT TCCACGAGAA TATCGACTGC ATCGAGAAGC TGTTGCAGCA GGATAGATCC 
CAGATTCTGG GCAATGTGGA GCGCATCTAC GATCTCATCG AGCGAGTTGC CGACCGACGC 
CCAGAGGCTT CCGTTCTAGC CCTAATCGAG TACCGATCCC GACGGGTTAC AGCAACGCGG 
CCAGATTGGC TTCAGGTGCT GGCCCAGTTC GTCCGGCGCT ACTACCGCAT GTCCAACGTT 
AACGTGCGCA TTAAGACGAT CGAGGCTCTA GTTCAGATTA TGGATCAAAA CCGTGCCTGC 
TATGAAGAAG AAATCCTTAG TCGCGTGGTG CTGGTGCACC TTTCCCATAT TCATCTGGAG 
TCGAGTGTGC AGGTGCGAGT GGCCGTGGCC CGGGCTCTTT CCAATTTTGC CACCCATTGC 
GACACGAAAC GGTGCATGGA TCTGCTAGAT ATCCTGGAGG CGCTCATCAA TCGACCCTTT 
GAGCACACGC GGCATGGATC CTCATCTGGA GAGGGTACAC TTGCAGAAGT GACCTTTGGA 
TTGGTCAACA ACGAATCCGA GATCTCGGAT ATTATCGCCG CAGTTGATGG TTTGGTCAAA 
GTGTTTGCCA TCAAGCTGCA TCGTCTGCCA GGGATCCATG CTCTTAAAAT ATTTAACATT 
CTAATGGACC ACCTGGAACT GCACTACGAA CGACCAAAGA TTTTCGAGCA CATAAGTGTT 
GTGCGCTATA AAATATTTGC TTGGTTGCTG AAGGCCCGGG CCAACGGCTC CTACCATATT 
GGTTATCCGG AGGGCAGCAC CGAGGTGGTG AAATTTAGTC CATACCTGGG AATTGATTCA 
CCACTGTTAC CACAAGCCCA CCCGACAGCG ATATCCATAC GACGAGTCTG CAAGCTGATA 
GTGAGGTGCC TGGAACACGA CACCGATTAT CAGGTGTTCC AGCTAGTCAT CAGGGAACTG 
CCGAAGGTGC TGCAGAACAA GGCGCTGGTG CAGGGCAATG ACATCGAGGA GCTGGCCAAC 
ACGCTCCTCA AGATCAATTT GGTTAGCAAC AACAAGTTCA AGCGACCCAC CGATGAGTTC 
CACGCCCTCG TTTTACCCGC CATAGCTTCA TTGGTTATCT ATCACGAAAG CCTGCAGCCG 
CAGCAGCACT ACGGAATTAT AACAGCTCTC AATTCGAGGG TACTTACTGG CATTGCCAGC 
GTCTGCATCA ACACCATGAC CATCCTTATA CTAGAGATGC CGGAGGCGTT GATGCGAAAG 
CTGCCGGACG TACTGCTCCA AATGAGTAAG ATGTCGGATA CGAATGCGTT GGCCACTCCG 
GTTCTGGAAT TTCTGTCGTT GCTCATACAT CTGTCCAAGC ATCTCTTCGC AAACTTCACT 
TCCATGCACA ACATGTACGT GTTTGCCATC ACGCTGCCGT ACACCAAACC GCATCGCTAC 
GATCACTATA CAGTTTCCTT GGCCCACCAT GTCATCGCTG GATGGTTCCT TAAGTGTAAG 
CTGGAGCTGC GCAGGAACTG CGTTAACTAC ATCAAAAGCT CGATTCAGTC GAATGCCAAG 
ATGTTGTCCA GCGACATTGT GAACCTAAAC TCCTTAAACG AGGATTCATC CAATCGCAAG 
CGGAGCACCA GTCTAACTGA GCGCGGTAGT CGCAACAATG CCAATGCCTG GAACGATCTG 
GAGATGCGAC CGCAAATGAA CAACGGTCTA CGAAACTTCC ACGCCGAACT GGCAGAGACG 
TGCTTTGATT TCTTGGCGCG ACACACTTAT TCGCCTTGTC CGTCGATGCC CAAGCGTCTT 
CCAGCGGCGG AATTCCTGCT GAAGGACGGC GTCTCGCAGA CGTGGCTAGT GGGCAACAAC 
CTGGTGACCA TAACCACCTC CGGCTGTCCG TCGGCGCCCG CGCGCAGTGG GCTTTGCGAA 
CGATGTGCCC AGCTAGGAAA GGCTCCCAGC ATTAGTCTGA ACTCGAAGAG CCTCAGTGAT 
GCAGCTCCTC CACTTTCGCC AGAGCGGGAG AGACGATACA CCAAGGTGAG CCTGCAGCAC 
AGCAGCGGCA ACGAGTCGGC TGGCAGCACG GAGCTCACCT CCTCCTCGTC GTCGAACTCA 
GCAGCCGCAG GTGGTCATCC ACATCGACAG ATTTCTAACA GTTCCACGGC ATCGCTAGAC 
GCGCTTTCCC GTCGGGGGTC TAATCCCGAA GCCTTGGGCA GTGCCTTGGG CGAGGGGGCA 
CATACGGGGA GCAACACCTC GCTCCTGGGA AACTCTTTGT CCCAATCCTC CGTTAGCATG 
AGCCCTTCGT CGGGATCCGT GGTGCAGCAG CCGGTTTGCG TGCGCGCCTG CACTGGCTGG 
GCGGAGGTTT TGGTTCGTCG GCCCACGGGC AACGTATCGT GGATAACCCG CATTCAGAAC 
CCCATCACCA ACGATTGCTT TGGCCAGGAG TTGCCGTTCA ATAATGTGGT GTCGCTGTTT 
CTTCCCACTG CCCATGGCGG AGTTTTTGGA CCTGACAATA CCATTCAGGC CCCACCACAT 
ACGTTGGCTG ATCCCGAACC AGAACCAGAA GCAGAGGTTA ATGCAGCAAC AGCTCCTCAG 
GCAAGTGCAT TGCGCAGCCG AATGGTTGCC AAGGCTCGTG CCCTTCAGCG ACAGGAAGAA 
ATTCACTCGG TCGGAGGAAA TGGAAATGGC AACGGCAACG GTAATGGTAG TGGAAATGCT 
CCCAGCACTG GAGGTGCTGC TGCCATACCC ATTCCGCGGG TCCCAGCATC TGGAAAACGC 
GGCAAGGAGG CAGCTCTTTG TGGAAGTGTG AGTGATGGTG AAGCCGACGA TGACTCCCTG 
GCCTTTGAGG ATGCTCAGAG TCGGGCACGA AATCCCGTCC GACGGGTAAA TTCCAGCCCA 
GAGATGAGTT CCAGTTGGCG GCAGTCGTTC CTAACCAACA AACCCACGCC ACTGTCCCAG 
GAGCCCGTGA AAACCACCGC CGATGAACCG CAATCGCTGT TGACTTTGAA GAAGAAGACT 
GTTCAGTACA GCACCAAGGA TATGCGTGTG AGCTGTGAGG CCATTCCGGA GGAGATCGCC 
GGCTCCACAC CACCGTCGCA GGCCGCAGCC TTGGCCCTGC AGCCGGAAAG CGGCACCCTA 
CCTCCCAAGC AGCATTCGGC GGACGATGTC AGCAGTCATG TGGCTGGAGG AAGTAACTTG 
CAAGCAGGAG GGTCCACGCT AAAGCTGGGT AAACCACCGC TGTCGCCGGG GCAACCGCCT 
CTGCTGGGCA CTGGTCGAGT GACCAGCTTT GGTGGCACCT CTGTTCCGCA GCCCGGTGCA 
CTGGCAAAAT CGAGCAGCAG TGGCAGCAAT GGCACCAATG TTGGAGTGAT AACCTCCGAT 
TACGACAACG GCAACAATGG AAACGGAGAC ATGATGCGAG GTCGTTCGAA AACCATTTCG 
GTGGTGCGTG AAGTGAATAA CGGAAATACG CGTCCGCCGC CGGCCAGCAG TTTCCGGAAT 
TTCGGAGCCG CAAAGCCGCC CATCAACACC AAGCTGTGCA TGAACCCGAG CTTTATTTTC 
ATGCAACTGT ACACCACTGG CCAGCTGGGA GTTACGGATG AGCCTCTAAA GGTGGGTCCG 
GAAAACAGCA GCGCTGTCTC CCTTATCGAT TTAGTCCCAC CGTTCGAGAC GCACAAGATC 
GGCGTGCTGT ACGTGGGCCA AGGGCAGTGC AACAACGAGG TGGAGATCTT GCGCAATTCG 
CACGGAAGTG CCAGGTATGT GGAGTTCCTG CGTAACATCG GCACCCTGGT CAGTCTGAAG 
GAAGCTGAGC AGAACAATCT ATTTATAATG CTAGATAGAA ACGGAGCAGA TGGCAAGTTT 
GCCTATATCT GGAAGGATGA CATACTGCAG GTTACGTTCC ATGTCGCTAC GCTGATGCCC 
ACCAATCTGC AGGATGATCC CAACTGCAAC GAGAAGAAGA GCCACATTGG CAATGACTTT 
GTGAAGATCA TCTACAACGA AAGCGGCGAG GAGTACAATC TAAACACAAT ATCCGGCCAA 
TTCAACTACG CCTGCGTGAT CGTTGAGCCA CTTGACCTGA ACTCGAACAG GGTGTATGTG 
AAGGCGCGAT CGGAGATTTC GAAATTCGTG TGCCACGCAG AATACCGGAT AGTGTCCGAT 
CGCAGTGCTC CTCTTTTGGC CCGACAGATG GCGCTGCACG CCAATCTCGC GTCGCTAGTC 
TACCAAAGTG TGCAGAAAAA GCATCCGTAC GCATCGAATT GGCTAGAACG ATTGCGCAAA 
CTAAAGCGCC TGCGATCGAA GCTAATTGAG GGCCTAAACA GCCAGCAAAA ATCCGGAAGC 
GCCTCGAGCG GCATCGGAAT CAACGCCTCA GCCATTGGTT CGGATATGGA CGACCAGCGA 
GGTGACTTCA TTAAATACAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524177.1
CDS	129..5672
Translation	MNSKDKSKFKLFLKSLPAGYVGERTLRPEFERELRPEQPVAQRCRMLKELGDTQLHNFNLDESAITILFNLTNDLIVPNKPAETRQIALSFYKRLIHTQYKNLTIMREKFFLVIQNHEAREDLRHLLELLDTLTDNGKDITNFEEKIGKFMLLWIPAITEANLLTPYLDILVNLIKFNAAHLDKDILVGIVQNACDLSCSVTVNEIGLQCLTILEMVIGYTIFPSEPLPQCITTLCRTVNHAPYCPSSFKIMKNLLGTQLGYHSMKMMCSILNDRALYDDAHLLRGAVFHLNMNIFGSNIIFQVSPMTYATNVLTAFLRALDSRQVIVTFEVILSVRMVITKRQLSEIIWDLICDIMSSIVSNIEYYEEVNINKDRLHHLQINFHENIDCIEKLLQQDRSQILGNVERIYDLIERVADRRPEASVLALIEYRSRRVTATRPDWLQVLAQFVRRYYRMSNVNVRIKTIEALVQIMDQNRACYEEEILSRVVLVHLSHIHLESSVQVRVAVARALSNFATHCDTKRCMDLLDILEALINRPFEHTRHGSSSGEGTLAEVTFGLVNNESEISDIIAAVDGLVKVFAIKLHRLPGIHALKIFNILMDHLELHYERPKIFEHISVVRYKIFAWLLKARANGSYHIGYPEGSTEVVKFSPYLGIDSPLLPQAHPTAISIRRVCKLIVRCLEHDTDYQVFQLVIRELPKVLQNKALVQGNDIEELANTLLKINLVSNNKFKRPTDEFHALVLPAIASLVIYHESLQPQQHYGIITALNSRVLTGIASVCINTMTILILEMPEALMRKLPDVLLQMSKMSDTNALATPVLEFLSLLIHLSKHLFANFTSMHNMYVFAITLPYTKPHRYDHYTVSLAHHVIAGWFLKCKLELRRNCVNYIKSSIQSNAKMLSSDIVNLNSLNEDSSNRKRSTSLTERGSRNNANAWNDLEMRPQMNNGLRNFHAELAETCFDFLARHTYSPCPSMPKRLPAAEFLLKDGVSQTWLVGNNLVTITTSGCPSAPARSGLCERCAQLGKAPSISLNSKSLSDAAPPLSPERERRYTKVSLQHSSGNESAGSTELTSSSSSNSAAAGGHPHRQISNSSTASLDALSRRGSNPEALGSALGEGAHTGSNTSLLGNSLSQSSVSMSPSSGSVVQQPVCVRACTGWAEVLVRRPTGNVSWITRIQNPITNDCFGQELPFNNVVSLFLPTAHGGVFGPDNTIQAPPHTLADPEPEPEAEVNAATAPQASALRSRMVAKARALQRQEEIHSVGGNGNGNGNGNGSGNAPSTGGAAAIPIPRVPASGKRGKEAALCGSVSDGEADDDSLAFEDAQSRARNPVRRVNSSPEMSSSWRQSFLTNKPTPLSQEPVKTTADEPQSLLTLKKKTVQYSTKDMRVSCEAIPEEIAGSTPPSQAAALALQPESGTLPPKQHSADDVSSHVAGGSNLQAGGSTLKLGKPPLSPGQPPLLGTGRVTSFGGTSVPQPGALAKSSSSGSNGTNVGVITSDYDNGNNGNGDMMRGRSKTISVVREVNNGNTRPPPASSFRNFGAAKPPINTKLCMNPSFIFMQLYTTGQLGVTDEPLKVGPENSSAVSLIDLVPPFETHKIGVLYVGQGQCNNEVEILRNSHGSARYVEFLRNIGTLVSLKEAEQNNLFIMLDRNGADGKFAYIWKDDILQVTFHVATLMPTNLQDDPNCNEKKSHIGNDFVKIIYNESGEEYNLNTISGQFNYACVIVEPLDLNSNRVYVKARSEISKFVCHAEYRIVSDRSAPLLARQMALHANLASLVYQSVQKKHPYASNWLERLRKLKRLRSKLIEGLNSQQKSGSASSGIGINASAIGSDMDDQRGDFIKYT

Target ORF information:

RefSeq Version	NM_079453.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gigas (gig), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079453.3

ORF Insert Sequence:

ATGAACTCCA AGGACAAGTC CAAGTTCAAG TTGTTCCTCA AATCGCTGCC GGCAGGTTAC 
GTGGGCGAGC GGACCCTGCG GCCGGAGTTT GAGAGGGAAC TGCGTCCGGA GCAGCCGGTG 
GCCCAGCGCT GCCGGATGCT GAAAGAGCTG GGCGACACGC AGCTGCACAA CTTCAATCTG 
GACGAGTCCG CCATCACCAT TCTGTTCAAT CTCACCAACG ATCTCATCGT GCCCAACAAG 
CCGGCAGAAA CGCGGCAGAT TGCCTTGAGC TTCTATAAAC GGCTCATTCA CACACAGTAC 
AAGAACCTGA CCATCATGCG GGAGAAGTTC TTCCTGGTCA TCCAGAACCA CGAAGCCCGC 
GAAGATCTGC GTCACCTTCT TGAGCTGCTC GACACCCTCA CCGACAATGG GAAGGATATC 
ACAAACTTCG AGGAGAAGAT TGGCAAGTTT ATGCTGCTCT GGATCCCAGC CATTACGGAG 
GCCAATCTGC TTACCCCCTA CCTTGACATT CTGGTCAACC TGATCAAGTT CAATGCGGCG 
CATCTGGACA AAGACATCCT TGTGGGCATC GTGCAGAACG CTTGCGATCT GAGCTGTAGC 
GTTACCGTAA ACGAAATCGG TCTGCAGTGC CTCACAATTC TGGAGATGGT CATCGGGTAC 
ACCATCTTCC CAAGCGAACC GCTGCCGCAG TGCATCACCA CCTTGTGCCG GACTGTGAAT 
CATGCTCCTT ACTGCCCGTC TTCTTTCAAG ATCATGAAGA ACCTCTTGGG CACCCAGTTG 
GGATACCATT CCATGAAGAT GATGTGCAGT ATCCTCAACG ATCGAGCTCT TTACGATGAT 
GCCCACCTGT TGCGTGGGGC CGTGTTCCAT CTAAACATGA ACATCTTTGG CTCGAACATT 
ATTTTCCAGG TTTCGCCTAT GACCTATGCA ACGAATGTGC TGACAGCCTT CCTGCGGGCA 
CTAGACAGTC GTCAGGTGAT CGTGACCTTC GAAGTCATCC TCAGCGTTCG CATGGTGATT 
ACCAAGCGGC AGCTTTCAGA GATCATTTGG GATCTGATCT GCGATATAAT GTCCTCGATC 
GTTAGCAATA TCGAGTATTA CGAAGAAGTC AATATAAACA AGGATCGTCT GCATCACCTG 
CAGATCAATT TCCACGAGAA TATCGACTGC ATCGAGAAGC TGTTGCAGCA GGATAGATCC 
CAGATTCTGG GCAATGTGGA GCGCATCTAC GATCTCATCG AGCGAGTTGC CGACCGACGC 
CCAGAGGCTT CCGTTCTAGC CCTAATCGAG TACCGATCCC GACGGGTTAC AGCAACGCGG 
CCAGATTGGC TTCAGGTGCT GGCCCAGTTC GTCCGGCGCT ACTACCGCAT GTCCAACGTT 
AACGTGCGCA TTAAGACGAT CGAGGCTCTA GTTCAGATTA TGGATCAAAA CCGTGCCTGC 
TATGAAGAAG AAATCCTTAG TCGCGTGGTG CTGGTGCACC TTTCCCATAT TCATCTGGAG 
TCGAGTGTGC AGGTGCGAGT GGCCGTGGCC CGGGCTCTTT CCAATTTTGC CACCCATTGC 
GACACGAAAC GGTGCATGGA TCTGCTAGAT ATCCTGGAGG CGCTCATCAA TCGACCCTTT 
GAGCACACGC GGCATGGATC CTCATCTGGA GAGGGTACAC TTGCAGAAGT GACCTTTGGA 
TTGGTCAACA ACGAATCCGA GATCTCGGAT ATTATCGCCG CAGTTGATGG TTTGGTCAAA 
GTGTTTGCCA TCAAGCTGCA TCGTCTGCCA GGGATCCATG CTCTTAAAAT ATTTAACATT 
CTAATGGACC ACCTGGAACT GCACTACGAA CGACCAAAGA TTTTCGAGCA CATAAGTGTT 
GTGCGCTATA AAATATTTGC TTGGTTGCTG AAGGCCCGGG CCAACGGCTC CTACCATATT 
GGTTATCCGG AGGGCAGCAC CGAGGTGGTG AAATTTAGTC CATACCTGGG AATTGATTCA 
CCACTGTTAC CACAAGCCCA CCCGACAGCG ATATCCATAC GACGAGTCTG CAAGCTGATA 
GTGAGGTGCC TGGAACACGA CACCGATTAT CAGGTGTTCC AGCTAGTCAT CAGGGAACTG 
CCGAAGGTGC TGCAGAACAA GGCGCTGGTG CAGGGCAATG ACATCGAGGA GCTGGCCAAC 
ACGCTCCTCA AGATCAATTT GGTTAGCAAC AACAAGTTCA AGCGACCCAC CGATGAGTTC 
CACGCCCTCG TTTTACCCGC CATAGCTTCA TTGGTTATCT ATCACGAAAG CCTGCAGCCG 
CAGCAGCACT ACGGAATTAT AACAGCTCTC AATTCGAGGG TACTTACTGG CATTGCCAGC 
GTCTGCATCA ACACCATGAC CATCCTTATA CTAGAGATGC CGGAGGCGTT GATGCGAAAG 
CTGCCGGACG TACTGCTCCA AATGAGTAAG ATGTCGGATA CGAATGCGTT GGCCACTCCG 
GTTCTGGAAT TTCTGTCGTT GCTCATACAT CTGTCCAAGC ATCTCTTCGC AAACTTCACT 
TCCATGCACA ACATGTACGT GTTTGCCATC ACGCTGCCGT ACACCAAACC GCATCGCTAC 
GATCACTATA CAGTTTCCTT GGCCCACCAT GTCATCGCTG GATGGTTCCT TAAGTGTAAG 
CTGGAGCTGC GCAGGAACTG CGTTAACTAC ATCAAAAGCT CGATTCAGTC GAATGCCAAG 
ATGTTGTCCA GCGACATTGT GAACCTAAAC TCCTTAAACG AGGATTCATC CAATCGCAAG 
CGGAGCACCA GTCTAACTGA GCGCGGTAGT CGCAACAATG CCAATGCCTG GAACGATCTG 
GAGATGCGAC CGCAAATGAA CAACGGTCTA CGAAACTTCC ACGCCGAACT GGCAGAGACG 
TGCTTTGATT TCTTGGCGCG ACACACTTAT TCGCCTTGTC CGTCGATGCC CAAGCGTCTT 
CCAGCGGCGG AATTCCTGCT GAAGGACGGC GTCTCGCAGA CGTGGCTAGT GGGCAACAAC 
CTGGTGACCA TAACCACCTC CGGCTGTCCG TCGGCGCCCG CGCGCAGTGG GCTTTGCGAA 
CGATGTGCCC AGCTAGGAAA GGCTCCCAGC ATTAGTCTGA ACTCGAAGAG CCTCAGTGAT 
GCAGCTCCTC CACTTTCGCC AGAGCGGGAG AGACGATACA CCAAGGTGAG CCTGCAGCAC 
AGCAGCGGCA ACGAGTCGGC TGGCAGCACG GAGCTCACCT CCTCCTCGTC GTCGAACTCA 
GCAGCCGCAG GTGGTCATCC ACATCGACAG ATTTCTAACA GTTCCACGGC ATCGCTAGAC 
GCGCTTTCCC GTCGGGGGTC TAATCCCGAA GCCTTGGGCA GTGCCTTGGG CGAGGGGGCA 
CATACGGGGA GCAACACCTC GCTCCTGGGA AACTCTTTGT CCCAATCCTC CGTTAGCATG 
AGCCCTTCGT CGGGATCCGT GGTGCAGCAG CCGGTTTGCG TGCGCGCCTG CACTGGCTGG 
GCGGAGGTTT TGGTTCGTCG GCCCACGGGC AACGTATCGT GGATAACCCG CATTCAGAAC 
CCCATCACCA ACGATTGCTT TGGCCAGGAG TTGCCGTTCA ATAATGTGGT GTCGCTGTTT 
CTTCCCACTG CCCATGGCGG AGTTTTTGGA CCTGACAATA CCATTCAGGC CCCACCACAT 
ACGTTGGCTG ATCCCGAACC AGAACCAGAA GCAGAGGTTA ATGCAGCAAC AGCTCCTCAG 
GCAAGTGCAT TGCGCAGCCG AATGGTTGCC AAGGCTCGTG CCCTTCAGCG ACAGGAAGAA 
ATTCACTCGG TCGGAGGAAA TGGAAATGGC AACGGCAACG GTAATGGTAG TGGAAATGCT 
CCCAGCACTG GAGGTGCTGC TGCCATACCC ATTCCGCGGG TCCCAGCATC TGGAAAACGC 
GGCAAGGAGG CAGCTCTTTG TGGAAGTGTG AGTGATGGTG AAGCCGACGA TGACTCCCTG 
GCCTTTGAGG ATGCTCAGAG TCGGGCACGA AATCCCGTCC GACGGGTAAA TTCCAGCCCA 
GAGATGAGTT CCAGTTGGCG GCAGTCGTTC CTAACCAACA AACCCACGCC ACTGTCCCAG 
GAGCCCGTGA AAACCACCGC CGATGAACCG CAATCGCTGT TGACTTTGAA GAAGAAGACT 
GTTCAGTACA GCACCAAGGA TATGCGTGTG AGCTGTGAGG CCATTCCGGA GGAGATCGCC 
GGCTCCACAC CACCGTCGCA GGCCGCAGCC TTGGCCCTGC AGCCGGAAAG CGGCACCCTA 
CCTCCCAAGC AGCATTCGGC GGACGATGTC AGCAGTCATG TGGCTGGAGG AAGTAACTTG 
CAAGCAGGAG GGTCCACGCT AAAGCTGGGT AAACCACCGC TGTCGCCGGG GCAACCGCCT 
CTGCTGGGCA CTGGTCGAGT GACCAGCTTT GGTGGCACCT CTGTTCCGCA GCCCGGTGCA 
CTGGCAAAAT CGAGCAGCAG TGGCAGCAAT GGCACCAATG TTGGAGTGAT AACCTCCGAT 
TACGACAACG GCAACAATGG AAACGGAGAC ATGATGCGAG GTCGTTCGAA AACCATTTCG 
GTGGTGCGTG AAGTGAATAA CGGAAATACG CGTCCGCCGC CGGCCAGCAG TTTCCGGAAT 
TTCGGAGCCG CAAAGCCGCC CATCAACACC AAGCTGTGCA TGAACCCGAG CTTTATTTTC 
ATGCAACTGT ACACCACTGG CCAGCTGGGA GTTACGGATG AGCCTCTAAA GGTGGGTCCG 
GAAAACAGCA GCGCTGTCTC CCTTATCGAT TTAGTCCCAC CGTTCGAGAC GCACAAGATC 
GGCGTGCTGT ACGTGGGCCA AGGGCAGTGC AACAACGAGG TGGAGATCTT GCGCAATTCG 
CACGGAAGTG CCAGGTATGT GGAGTTCCTG CGTAACATCG GCACCCTGGT CAGTCTGAAG 
GAAGCTGAGC AGAACAATCT ATTTATAATG CTAGATAGAA ACGGAGCAGA TGGCAAGTTT 
GCCTATATCT GGAAGGATGA CATACTGCAG GTTACGTTCC ATGTCGCTAC GCTGATGCCC 
ACCAATCTGC AGGATGATCC CAACTGCAAC GAGAAGAAGA GCCACATTGG CAATGACTTT 
GTGAAGATCA TCTACAACGA AAGCGGCGAG GAGTACAATC TAAACACAAT ATCCGGCCAA 
TTCAACTACG CCTGCGTGAT CGTTGAGCCA CTTGACCTGA ACTCGAACAG GGTGTATGTG 
AAGGCGCGAT CGGAGATTTC GAAATTCGTG TGCCACGCAG AATACCGGAT AGTGTCCGAT 
CGCAGTGCTC CTCTTTTGGC CCGACAGATG GCGCTGCACG CCAATCTCGC GTCGCTAGTC 
TACCAAAGTG TGCAGAAAAA GCATCCGTAC GCATCGAATT GGCTAGAACG ATTGCGCAAA 
CTAAAGCGCC TGCGATCGAA GCTAATTGAG GGCCTAAACA GCCAGCAAAA ATCCGGAAGC 
GCCTCGAGCG GCATCGGAAT CAACGCCTCA GCCATTGGTT CGGATATGGA CGACCAGCGA 
GGTGACTTCA TTAAATACAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gig ( NM_001275160.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262089.1 Drosophila melanogaster gigas (gig), transcript variant B, mRNA.

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CloneID	OFa21111
Clone ID Related Accession (Same CDS sequence)	NM_001275160.1
Accession Version	NM_001275160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gigas, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCA AGGACAAGTC CAAGTTCAAG TTGTTCCTCA AATCGCTGCC GGCAGGTTAC 
GTGGGCGAGC GGACCCTGCG GCCGGAGTTT GAGAGGGAAC TGCGTCCGGA GCAGCCGGTG 
GCCCAGCGCT GCCGGATGCT GAAAGAGCTG GGCGACACGC AGCTGCACAA CTTCAATCTG 
GACGAGTCCG CCATCACCAT TCTGTTCAAT CTCACCAACG ATCTCATCGT GCCCAACAAG 
CCGGCAGAAA CGCGGCAGAT TGCCTTGAGC TTCTATAAAC GGCTCATTCA CACACAGTAC 
AAGAACCTGA CCATCATGCG GGAGAAGTTC TTCCTGGTCA TCCAGAACCA CGAAGCCCGC 
GAAGATCTGC GTCACCTTCT TGAGCTGCTC GACACCCTCA CCGACAATGG GAAGGATATC 
ACAAACTTCG AGGAGAAGAT TGGCAAGTTT ATGCTGCTCT GGATCCCAGC CATTACGGAG 
GCCAATCTGC TTACCCCCTA CCTTGACATT CTGGTCAACC TGATCAAGTT CAATGCGGCG 
CATCTGGACA AAGACATCCT TGTGGGCATC GTGCAGAACG CTTGCGATCT GAGCTGTAGC 
GTTACCGTAA ACGAAATCGG TCTGCAGTGC CTCACAATTC TGGAGATGGT CATCGGGTAC 
ACCATCTTCC CAAGCGAACC GCTGCCGCAG TGCATCACCA CCTTGTGCCG GACTGTGAAT 
CATGCTCCTT ACTGCCCGTC TTCTTTCAAG ATCATGAAGA ACCTCTTGGG CACCCAGTTG 
GGATACCATT CCATGAAGAT GATGTGCAGT ATCCTCAACG ATCGAGCTCT TTACGATGAT 
GCCCACCTGT TGCGTGGGGC CGTGTTCCAT CTAAACATGA ACATCTTTGG CTCGAACATT 
ATTTTCCAGG TTTCGCCTAT GACCTATGCA ACGAATGTGC TGACAGCCTT CCTGCGGGCA 
CTAGACAGTC GTCAGGTGAT CGTGACCTTC GAAGTCATCC TCAGCGTTCG CATGGTGATT 
ACCAAGCGGC AGCTTTCAGA GATCATTTGG GATCTGATCT GCGATATAAT GTCCTCGATC 
GTTAGCAATA TCGAGTATTA CGAAGAAGTC AATATAAACA AGGATCGTCT GCATCACCTG 
CAGATCAATT TCCACGAGAA TATCGACTGC ATCGAGAAGC TGTTGCAGCA GGATAGATCC 
CAGATTCTGG GCAATGTGGA GCGCATCTAC GATCTCATCG AGCGAGTTGC CGACCGACGC 
CCAGAGGCTT CCGTTCTAGC CCTAATCGAG TACCGATCCC GACGGGTTAC AGCAACGCGG 
CCAGATTGGC TTCAGGTGCT GGCCCAGTTC GTCCGGCGCT ACTACCGCAT GTCCAACGTT 
AACGTGCGCA TTAAGACGAT CGAGGCTCTA GTTCAGATTA TGGATCAAAA CCGTGCCTGC 
TATGAAGAAG AAATCCTTAG TCGCGTGGTG CTGGTGCACC TTTCCCATAT TCATCTGGAG 
TCGAGTGTGC AGGTGCGAGT GGCCGTGGCC CGGGCTCTTT CCAATTTTGC CACCCATTGC 
GACACGAAAC GGTGCATGGA TCTGCTAGAT ATCCTGGAGG CGCTCATCAA TCGACCCTTT 
GAGCACACGC GGCATGGATC CTCATCTGGA GAGGGTACAC TTGCAGAAGT GACCTTTGGA 
TTGGTCAACA ACGAATCCGA GATCTCGGAT ATTATCGCCG CAGTTGATGG TTTGGTCAAA 
GTGTTTGCCA TCAAGCTGCA TCGTCTGCCA GGGATCCATG CTCTTAAAAT ATTTAACATT 
CTAATGGACC ACCTGGAACT GCACTACGAA CGACCAAAGA TTTTCGAGCA CATAAGTGTT 
GTGCGCTATA AAATATTTGC TTGGTTGCTG AAGGCCCGGG CCAACGGCTC CTACCATATT 
GGTTATCCGG AGGGCAGCAC CGAGGTGGTG AAATTTAGTC CATACCTGGG AATTGATTCA 
CCACTGTTAC CACAAGCCCA CCCGACAGCG ATATCCATAC GACGAGTCTG CAAGCTGATA 
GTGAGGTGCC TGGAACACGA CACCGATTAT CAGGTGTTCC AGCTAGTCAT CAGGGAACTG 
CCGAAGGTGC TGCAGAACAA GGCGCTGGTG CAGGGCAATG ACATCGAGGA GCTGGCCAAC 
ACGCTCCTCA AGATCAATTT GGTTAGCAAC AACAAGTTCA AGCGACCCAC CGATGAGTTC 
CACGCCCTCG TTTTACCCGC CATAGCTTCA TTGGTTATCT ATCACGAAAG CCTGCAGCCG 
CAGCAGCACT ACGGAATTAT AACAGCTCTC AATTCGAGGG TACTTACTGG CATTGCCAGC 
GTCTGCATCA ACACCATGAC CATCCTTATA CTAGAGATGC CGGAGGCGTT GATGCGAAAG 
CTGCCGGACG TACTGCTCCA AATGAGTAAG ATGTCGGATA CGAATGCGTT GGCCACTCCG 
GTTCTGGAAT TTCTGTCGTT GCTCATACAT CTGTCCAAGC ATCTCTTCGC AAACTTCACT 
TCCATGCACA ACATGTACGT GTTTGCCATC ACGCTGCCGT ACACCAAACC GCATCGCTAC 
GATCACTATA CAGTTTCCTT GGCCCACCAT GTCATCGCTG GATGGTTCCT TAAGTGTAAG 
CTGGAGCTGC GCAGGAACTG CGTTAACTAC ATCAAAAGCT CGAATGCCAA GATGTTGTCC 
AGCGACATTG TGAACCTAAA CTCCTTAAAC GAGGATTCAT CCAATCGCAA GCGGAGCACC 
AGTCTAACTG AGCGCGGTAG TCGCAACAAT GCCAATGCCT GGAACGATCT GGAGATGCGA 
CCGCAAATGA ACAACGGTCT ACGAAACTTC CACGCCGAAC TGGCAGAGAC GTGCTTTGAT 
TTCTTGGCGC GACACACTTA TTCGCCTTGT CCGTCGATGC CCAAGCGTCT TCCAGCGGCG 
GAATTCCTGC TGAAGGACGG CGTCTCGCAG ACGTGGCTAG TGGGCAACAA CCTGGTGACC 
ATAACCACCT CCGGCTGTCC GTCGGCGCCC GCGCGCAGTG GGCTTTGCGA ACGATGTGCC 
CAGCTAGGAA AGGCTCCCAG CATTAGTCTG AACTCGAAGA GCCTCAGTGA TGCAGCTCCT 
CCACTTTCGC CAGAGCGGGA GAGACGATAC ACCAAGGTGA GCCTGCAGCA CAGCAGCGGC 
AACGAGTCGG CTGGCAGCAC GGAGCTCACC TCCTCCTCGT CGTCGAACTC AGCAGCCGCA 
GGTGGTCATC CACATCGACA GATTTCTAAC AGTTCCACGG CATCGCTAGA CGCGCTTTCC 
CGTCGGGGGT CTAATCCCGA AGCCTTGGGC AGTGCCTTGG GCGAGGGGGC ACATACGGGG 
AGCAACACCT CGCTCCTGGG AAACTCTTTG TCCCAATCCT CCGTTAGCAT GAGCCCTTCG 
TCGGGATCCG TGGTGCAGCA GCCGGTTTGC GTGCGCGCCT GCACTGGCTG GGCGGAGGTT 
TTGGTTCGTC GGCCCACGGG CAACGTATCG TGGATAACCC GCATTCAGAA CCCCATCACC 
AACGATTGCT TTGGCCAGGA GTTGCCGTTC AATAATGTGG TGTCGCTGTT TCTTCCCACT 
GCCCATGGCG GAGTTTTTGG ACCTGACAAT ACCATTCAGG CCCCACCACA TACGTTGGCT 
GATCCCGAAC CAGAACCAGA AGCAGAGGTT AATGCAGCAA CAGCTCCTCA GGCAAGTGCA 
TTGCGCAGCC GAATGGTTGC CAAGGCTCGT GCCCTTCAGC GACAGGAAGA AATTCACTCG 
GTCGGAGGAA ATGGAAATGG CAACGGCAAC GGTAATGGTA GTGGAAATGC TCCCAGCACT 
GGAGGTGCTG CTGCCATACC CATTCCGCGG GTCCCAGCAT CTGGAAAACG CGGCAAGGAG 
GCAGCTCTTT GTGGAAGTGT GAGTGATGGT GAAGCCGACG ATGACTCCCT GGCCTTTGAG 
GATGCTCAGA GTCGGGCACG AAATCCCGTC CGACGGGTAA ATTCCAGCCC AGAGATGAGT 
TCCAGTTGGC GGCAGTCGTT CCTAACCAAC AAACCCACGC CACTGTCCCA GGAGCCCGTG 
AAAACCACCG CCGATGAACC GCAATCGCTG TTGACTTTGA AGAAGAAGAC TGTTCAGTAC 
AGCACCAAGG ATATGCGTGT GAGCTGTGAG GCCATTCCGG AGGAGATCGC CGGCTCCACA 
CCACCGTCGC AGGCCGCAGC CTTGGCCCTG CAGCCGGAAA GCGGCACCCT ACCTCCCAAG 
CAGCATTCGG CGGACGATGT CAGCAGTCAT GTGGCTGGAG GAAGTAACTT GCAAGCAGGA 
GGGTCCACGC TAAAGCTGGG TAAACCACCG CTGTCGCCGG GGCAACCGCC TCTGCTGGGC 
ACTGGTCGAG TGACCAGCTT TGGTGGCACC TCTGTTCCGC AGCCCGGTGC ACTGGCAAAA 
TCGAGCAGCA GTGGCAGCAA TGGCACCAAT GTTGGAGTGA TAACCTCCGA TTACGACAAC 
GGCAACAATG GAAACGGAGA CATGATGCGA GGTCGTTCGA AAACCATTTC GGTGGTGCGT 
GAAGTGAATA ACGGAAATAC GCGTCCGCCG CCGGCCAGCA GTTTCCGGAA TTTCGGAGCC 
GCAAAGCCGC CCATCAACAC CAAGCTGTGC ATGAACCCGA GCTTTATTTT CATGCAACTG 
TACACCACTG GCCAGCTGGG AGTTACGGAT GAGCCTCTAA AGGTGGGTCC GGAAAACAGC 
AGCGCTGTCT CCCTTATCGA TTTAGTCCCA CCGTTCGAGA CGCACAAGAT CGGCGTGCTG 
TACGTGGGCC AAGGGCAGTG CAACAACGAG GTGGAGATCT TGCGCAATTC GCACGGAAGT 
GCCAGGTATG TGGAGTTCCT GCGTAACATC GGCACCCTGG TCAGTCTGAA GGAAGCTGAG 
CAGAACAATC TATTTATAAT GCTAGATAGA AACGGAGCAG ATGGCAAGTT TGCCTATATC 
TGGAAGGATG ACATACTGCA GGTTACGTTC CATGTCGCTA CGCTGATGCC CACCAATCTG 
CAGGATGATC CCAACTGCAA CGAGAAGAAG AGCCACATTG GCAATGACTT TGTGAAGATC 
ATCTACAACG AAAGCGGCGA GGAGTACAAT CTAAACACAA TATCCGGCCA ATTCAACTAC 
GCCTGCGTGA TCGTTGAGCC ACTTGACCTG AACTCGAACA GGGTGTATGT GAAGGCGCGA 
TCGGAGATTT CGAAATTCGT GTGCCACGCA GAATACCGGA TAGTGTCCGA TCGCAGTGCT 
CCTCTTTTGG CCCGACAGAT GGCGCTGCAC GCCAATCTCG CGTCGCTAGT CTACCAAAGT 
GTGCAGAAAA AGCATCCGTA CGCATCGAAT TGGCTAGAAC GATTGCGCAA ACTAAAGCGC 
CTGCGATCGA AGCTAATTGA GGGCCTAAAC AGCCAGCAAA AATCCGGAAG CGCCTCGAGC 
GGCATCGGAA TCAACGCCTC AGCCATTGGT TCGGATATGG ACGACCAGCG AGGTGACTTC 
ATTAAATACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262089.1
CDS	129..5663
Translation	MNSKDKSKFKLFLKSLPAGYVGERTLRPEFERELRPEQPVAQRCRMLKELGDTQLHNFNLDESAITILFNLTNDLIVPNKPAETRQIALSFYKRLIHTQYKNLTIMREKFFLVIQNHEAREDLRHLLELLDTLTDNGKDITNFEEKIGKFMLLWIPAITEANLLTPYLDILVNLIKFNAAHLDKDILVGIVQNACDLSCSVTVNEIGLQCLTILEMVIGYTIFPSEPLPQCITTLCRTVNHAPYCPSSFKIMKNLLGTQLGYHSMKMMCSILNDRALYDDAHLLRGAVFHLNMNIFGSNIIFQVSPMTYATNVLTAFLRALDSRQVIVTFEVILSVRMVITKRQLSEIIWDLICDIMSSIVSNIEYYEEVNINKDRLHHLQINFHENIDCIEKLLQQDRSQILGNVERIYDLIERVADRRPEASVLALIEYRSRRVTATRPDWLQVLAQFVRRYYRMSNVNVRIKTIEALVQIMDQNRACYEEEILSRVVLVHLSHIHLESSVQVRVAVARALSNFATHCDTKRCMDLLDILEALINRPFEHTRHGSSSGEGTLAEVTFGLVNNESEISDIIAAVDGLVKVFAIKLHRLPGIHALKIFNILMDHLELHYERPKIFEHISVVRYKIFAWLLKARANGSYHIGYPEGSTEVVKFSPYLGIDSPLLPQAHPTAISIRRVCKLIVRCLEHDTDYQVFQLVIRELPKVLQNKALVQGNDIEELANTLLKINLVSNNKFKRPTDEFHALVLPAIASLVIYHESLQPQQHYGIITALNSRVLTGIASVCINTMTILILEMPEALMRKLPDVLLQMSKMSDTNALATPVLEFLSLLIHLSKHLFANFTSMHNMYVFAITLPYTKPHRYDHYTVSLAHHVIAGWFLKCKLELRRNCVNYIKSSNAKMLSSDIVNLNSLNEDSSNRKRSTSLTERGSRNNANAWNDLEMRPQMNNGLRNFHAELAETCFDFLARHTYSPCPSMPKRLPAAEFLLKDGVSQTWLVGNNLVTITTSGCPSAPARSGLCERCAQLGKAPSISLNSKSLSDAAPPLSPERERRYTKVSLQHSSGNESAGSTELTSSSSSNSAAAGGHPHRQISNSSTASLDALSRRGSNPEALGSALGEGAHTGSNTSLLGNSLSQSSVSMSPSSGSVVQQPVCVRACTGWAEVLVRRPTGNVSWITRIQNPITNDCFGQELPFNNVVSLFLPTAHGGVFGPDNTIQAPPHTLADPEPEPEAEVNAATAPQASALRSRMVAKARALQRQEEIHSVGGNGNGNGNGNGSGNAPSTGGAAAIPIPRVPASGKRGKEAALCGSVSDGEADDDSLAFEDAQSRARNPVRRVNSSPEMSSSWRQSFLTNKPTPLSQEPVKTTADEPQSLLTLKKKTVQYSTKDMRVSCEAIPEEIAGSTPPSQAAALALQPESGTLPPKQHSADDVSSHVAGGSNLQAGGSTLKLGKPPLSPGQPPLLGTGRVTSFGGTSVPQPGALAKSSSSGSNGTNVGVITSDYDNGNNGNGDMMRGRSKTISVVREVNNGNTRPPPASSFRNFGAAKPPINTKLCMNPSFIFMQLYTTGQLGVTDEPLKVGPENSSAVSLIDLVPPFETHKIGVLYVGQGQCNNEVEILRNSHGSARYVEFLRNIGTLVSLKEAEQNNLFIMLDRNGADGKFAYIWKDDILQVTFHVATLMPTNLQDDPNCNEKKSHIGNDFVKIIYNESGEEYNLNTISGQFNYACVIVEPLDLNSNRVYVKARSEISKFVCHAEYRIVSDRSAPLLARQMALHANLASLVYQSVQKKHPYASNWLERLRKLKRLRSKLIEGLNSQQKSGSASSGIGINASAIGSDMDDQRGDFIKYT

Target ORF information:

RefSeq Version	NM_001275160.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gigas (gig), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275160.1

ORF Insert Sequence:

ATGAACTCCA AGGACAAGTC CAAGTTCAAG TTGTTCCTCA AATCGCTGCC GGCAGGTTAC 
GTGGGCGAGC GGACCCTGCG GCCGGAGTTT GAGAGGGAAC TGCGTCCGGA GCAGCCGGTG 
GCCCAGCGCT GCCGGATGCT GAAAGAGCTG GGCGACACGC AGCTGCACAA CTTCAATCTG 
GACGAGTCCG CCATCACCAT TCTGTTCAAT CTCACCAACG ATCTCATCGT GCCCAACAAG 
CCGGCAGAAA CGCGGCAGAT TGCCTTGAGC TTCTATAAAC GGCTCATTCA CACACAGTAC 
AAGAACCTGA CCATCATGCG GGAGAAGTTC TTCCTGGTCA TCCAGAACCA CGAAGCCCGC 
GAAGATCTGC GTCACCTTCT TGAGCTGCTC GACACCCTCA CCGACAATGG GAAGGATATC 
ACAAACTTCG AGGAGAAGAT TGGCAAGTTT ATGCTGCTCT GGATCCCAGC CATTACGGAG 
GCCAATCTGC TTACCCCCTA CCTTGACATT CTGGTCAACC TGATCAAGTT CAATGCGGCG 
CATCTGGACA AAGACATCCT TGTGGGCATC GTGCAGAACG CTTGCGATCT GAGCTGTAGC 
GTTACCGTAA ACGAAATCGG TCTGCAGTGC CTCACAATTC TGGAGATGGT CATCGGGTAC 
ACCATCTTCC CAAGCGAACC GCTGCCGCAG TGCATCACCA CCTTGTGCCG GACTGTGAAT 
CATGCTCCTT ACTGCCCGTC TTCTTTCAAG ATCATGAAGA ACCTCTTGGG CACCCAGTTG 
GGATACCATT CCATGAAGAT GATGTGCAGT ATCCTCAACG ATCGAGCTCT TTACGATGAT 
GCCCACCTGT TGCGTGGGGC CGTGTTCCAT CTAAACATGA ACATCTTTGG CTCGAACATT 
ATTTTCCAGG TTTCGCCTAT GACCTATGCA ACGAATGTGC TGACAGCCTT CCTGCGGGCA 
CTAGACAGTC GTCAGGTGAT CGTGACCTTC GAAGTCATCC TCAGCGTTCG CATGGTGATT 
ACCAAGCGGC AGCTTTCAGA GATCATTTGG GATCTGATCT GCGATATAAT GTCCTCGATC 
GTTAGCAATA TCGAGTATTA CGAAGAAGTC AATATAAACA AGGATCGTCT GCATCACCTG 
CAGATCAATT TCCACGAGAA TATCGACTGC ATCGAGAAGC TGTTGCAGCA GGATAGATCC 
CAGATTCTGG GCAATGTGGA GCGCATCTAC GATCTCATCG AGCGAGTTGC CGACCGACGC 
CCAGAGGCTT CCGTTCTAGC CCTAATCGAG TACCGATCCC GACGGGTTAC AGCAACGCGG 
CCAGATTGGC TTCAGGTGCT GGCCCAGTTC GTCCGGCGCT ACTACCGCAT GTCCAACGTT 
AACGTGCGCA TTAAGACGAT CGAGGCTCTA GTTCAGATTA TGGATCAAAA CCGTGCCTGC 
TATGAAGAAG AAATCCTTAG TCGCGTGGTG CTGGTGCACC TTTCCCATAT TCATCTGGAG 
TCGAGTGTGC AGGTGCGAGT GGCCGTGGCC CGGGCTCTTT CCAATTTTGC CACCCATTGC 
GACACGAAAC GGTGCATGGA TCTGCTAGAT ATCCTGGAGG CGCTCATCAA TCGACCCTTT 
GAGCACACGC GGCATGGATC CTCATCTGGA GAGGGTACAC TTGCAGAAGT GACCTTTGGA 
TTGGTCAACA ACGAATCCGA GATCTCGGAT ATTATCGCCG CAGTTGATGG TTTGGTCAAA 
GTGTTTGCCA TCAAGCTGCA TCGTCTGCCA GGGATCCATG CTCTTAAAAT ATTTAACATT 
CTAATGGACC ACCTGGAACT GCACTACGAA CGACCAAAGA TTTTCGAGCA CATAAGTGTT 
GTGCGCTATA AAATATTTGC TTGGTTGCTG AAGGCCCGGG CCAACGGCTC CTACCATATT 
GGTTATCCGG AGGGCAGCAC CGAGGTGGTG AAATTTAGTC CATACCTGGG AATTGATTCA 
CCACTGTTAC CACAAGCCCA CCCGACAGCG ATATCCATAC GACGAGTCTG CAAGCTGATA 
GTGAGGTGCC TGGAACACGA CACCGATTAT CAGGTGTTCC AGCTAGTCAT CAGGGAACTG 
CCGAAGGTGC TGCAGAACAA GGCGCTGGTG CAGGGCAATG ACATCGAGGA GCTGGCCAAC 
ACGCTCCTCA AGATCAATTT GGTTAGCAAC AACAAGTTCA AGCGACCCAC CGATGAGTTC 
CACGCCCTCG TTTTACCCGC CATAGCTTCA TTGGTTATCT ATCACGAAAG CCTGCAGCCG 
CAGCAGCACT ACGGAATTAT AACAGCTCTC AATTCGAGGG TACTTACTGG CATTGCCAGC 
GTCTGCATCA ACACCATGAC CATCCTTATA CTAGAGATGC CGGAGGCGTT GATGCGAAAG 
CTGCCGGACG TACTGCTCCA AATGAGTAAG ATGTCGGATA CGAATGCGTT GGCCACTCCG 
GTTCTGGAAT TTCTGTCGTT GCTCATACAT CTGTCCAAGC ATCTCTTCGC AAACTTCACT 
TCCATGCACA ACATGTACGT GTTTGCCATC ACGCTGCCGT ACACCAAACC GCATCGCTAC 
GATCACTATA CAGTTTCCTT GGCCCACCAT GTCATCGCTG GATGGTTCCT TAAGTGTAAG 
CTGGAGCTGC GCAGGAACTG CGTTAACTAC ATCAAAAGCT CGAATGCCAA GATGTTGTCC 
AGCGACATTG TGAACCTAAA CTCCTTAAAC GAGGATTCAT CCAATCGCAA GCGGAGCACC 
AGTCTAACTG AGCGCGGTAG TCGCAACAAT GCCAATGCCT GGAACGATCT GGAGATGCGA 
CCGCAAATGA ACAACGGTCT ACGAAACTTC CACGCCGAAC TGGCAGAGAC GTGCTTTGAT 
TTCTTGGCGC GACACACTTA TTCGCCTTGT CCGTCGATGC CCAAGCGTCT TCCAGCGGCG 
GAATTCCTGC TGAAGGACGG CGTCTCGCAG ACGTGGCTAG TGGGCAACAA CCTGGTGACC 
ATAACCACCT CCGGCTGTCC GTCGGCGCCC GCGCGCAGTG GGCTTTGCGA ACGATGTGCC 
CAGCTAGGAA AGGCTCCCAG CATTAGTCTG AACTCGAAGA GCCTCAGTGA TGCAGCTCCT 
CCACTTTCGC CAGAGCGGGA GAGACGATAC ACCAAGGTGA GCCTGCAGCA CAGCAGCGGC 
AACGAGTCGG CTGGCAGCAC GGAGCTCACC TCCTCCTCGT CGTCGAACTC AGCAGCCGCA 
GGTGGTCATC CACATCGACA GATTTCTAAC AGTTCCACGG CATCGCTAGA CGCGCTTTCC 
CGTCGGGGGT CTAATCCCGA AGCCTTGGGC AGTGCCTTGG GCGAGGGGGC ACATACGGGG 
AGCAACACCT CGCTCCTGGG AAACTCTTTG TCCCAATCCT CCGTTAGCAT GAGCCCTTCG 
TCGGGATCCG TGGTGCAGCA GCCGGTTTGC GTGCGCGCCT GCACTGGCTG GGCGGAGGTT 
TTGGTTCGTC GGCCCACGGG CAACGTATCG TGGATAACCC GCATTCAGAA CCCCATCACC 
AACGATTGCT TTGGCCAGGA GTTGCCGTTC AATAATGTGG TGTCGCTGTT TCTTCCCACT 
GCCCATGGCG GAGTTTTTGG ACCTGACAAT ACCATTCAGG CCCCACCACA TACGTTGGCT 
GATCCCGAAC CAGAACCAGA AGCAGAGGTT AATGCAGCAA CAGCTCCTCA GGCAAGTGCA 
TTGCGCAGCC GAATGGTTGC CAAGGCTCGT GCCCTTCAGC GACAGGAAGA AATTCACTCG 
GTCGGAGGAA ATGGAAATGG CAACGGCAAC GGTAATGGTA GTGGAAATGC TCCCAGCACT 
GGAGGTGCTG CTGCCATACC CATTCCGCGG GTCCCAGCAT CTGGAAAACG CGGCAAGGAG 
GCAGCTCTTT GTGGAAGTGT GAGTGATGGT GAAGCCGACG ATGACTCCCT GGCCTTTGAG 
GATGCTCAGA GTCGGGCACG AAATCCCGTC CGACGGGTAA ATTCCAGCCC AGAGATGAGT 
TCCAGTTGGC GGCAGTCGTT CCTAACCAAC AAACCCACGC CACTGTCCCA GGAGCCCGTG 
AAAACCACCG CCGATGAACC GCAATCGCTG TTGACTTTGA AGAAGAAGAC TGTTCAGTAC 
AGCACCAAGG ATATGCGTGT GAGCTGTGAG GCCATTCCGG AGGAGATCGC CGGCTCCACA 
CCACCGTCGC AGGCCGCAGC CTTGGCCCTG CAGCCGGAAA GCGGCACCCT ACCTCCCAAG 
CAGCATTCGG CGGACGATGT CAGCAGTCAT GTGGCTGGAG GAAGTAACTT GCAAGCAGGA 
GGGTCCACGC TAAAGCTGGG TAAACCACCG CTGTCGCCGG GGCAACCGCC TCTGCTGGGC 
ACTGGTCGAG TGACCAGCTT TGGTGGCACC TCTGTTCCGC AGCCCGGTGC ACTGGCAAAA 
TCGAGCAGCA GTGGCAGCAA TGGCACCAAT GTTGGAGTGA TAACCTCCGA TTACGACAAC 
GGCAACAATG GAAACGGAGA CATGATGCGA GGTCGTTCGA AAACCATTTC GGTGGTGCGT 
GAAGTGAATA ACGGAAATAC GCGTCCGCCG CCGGCCAGCA GTTTCCGGAA TTTCGGAGCC 
GCAAAGCCGC CCATCAACAC CAAGCTGTGC ATGAACCCGA GCTTTATTTT CATGCAACTG 
TACACCACTG GCCAGCTGGG AGTTACGGAT GAGCCTCTAA AGGTGGGTCC GGAAAACAGC 
AGCGCTGTCT CCCTTATCGA TTTAGTCCCA CCGTTCGAGA CGCACAAGAT CGGCGTGCTG 
TACGTGGGCC AAGGGCAGTG CAACAACGAG GTGGAGATCT TGCGCAATTC GCACGGAAGT 
GCCAGGTATG TGGAGTTCCT GCGTAACATC GGCACCCTGG TCAGTCTGAA GGAAGCTGAG 
CAGAACAATC TATTTATAAT GCTAGATAGA AACGGAGCAG ATGGCAAGTT TGCCTATATC 
TGGAAGGATG ACATACTGCA GGTTACGTTC CATGTCGCTA CGCTGATGCC CACCAATCTG 
CAGGATGATC CCAACTGCAA CGAGAAGAAG AGCCACATTG GCAATGACTT TGTGAAGATC 
ATCTACAACG AAAGCGGCGA GGAGTACAAT CTAAACACAA TATCCGGCCA ATTCAACTAC 
GCCTGCGTGA TCGTTGAGCC ACTTGACCTG AACTCGAACA GGGTGTATGT GAAGGCGCGA 
TCGGAGATTT CGAAATTCGT GTGCCACGCA GAATACCGGA TAGTGTCCGA TCGCAGTGCT 
CCTCTTTTGG CCCGACAGAT GGCGCTGCAC GCCAATCTCG CGTCGCTAGT CTACCAAAGT 
GTGCAGAAAA AGCATCCGTA CGCATCGAAT TGGCTAGAAC GATTGCGCAA ACTAAAGCGC 
CTGCGATCGA AGCTAATTGA GGGCCTAAAC AGCCAGCAAA AATCCGGAAG CGCCTCGAGC 
GGCATCGGAA TCAACGCCTC AGCCATTGGT TCGGATATGG ACGACCAGCG AGGTGACTTC 
ATTAAATACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gig cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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