Tsc2 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Tsc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tsc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa57180 XM_006245909.3
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57181 XM_017597019.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57182 XM_017597020.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57183 XM_017597021.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57184 XM_017597022.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57185 XM_017597023.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57186 XM_017597024.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57187 XM_017597025.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57188 XM_017597026.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57189 XM_017597027.1
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa00599 NM_012680.3
Latest version!
Rattus norvegicus TSC complex subunit 2 (Tsc2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
ORa42979 XM_008767544.2
Latest version!
Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa57180
    Clone ID Related Accession (Same CDS sequence) XM_006245909.3
    Accession Version XM_006245909.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT
    CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CTCTTACCAG TGCCAGCTTG
    GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACCTGAAGAA TCTCCACTTG
    GAACTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA CTTCACTGCG
    GTTCCCAAGA GGTCCCCTGT GGGAGAATTC CTCCTGGCAG GGGGCAGGAC CAAAACGTGG
    CTGGTTGGAA ATAAGCTGGT CACTGTGACA ACGAGTGTGG GAACTGGTAC GCGGTCGTTG
    CTGGGCCTGG ACTCCGGGGA TCTGCAGGGT GGCTCAGCAT CAAGCTCTGA TCCTGGCACA
    CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG AGTCCGCTCC ATGTCAGGGG GCCACGGCCT TCGAGTTGGT
    GTCCTGGACA CTTCAGCTCC CTACACCCCA GGAGGCCCTG CTTCTCTGGG AGCACAGGCA
    GCACCAGCAG CAAGGCCTGA GAAGCCCTGT GCAGGAGCCC AACTTCCGGC AGCGGAGAAG
    GCGAACCTGG CAGCCTATGT GCCTTTGCTG ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC
    AGGCCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTTTCC
    TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCTAATG CCCTTATGGC TGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACA
    GCCAAGCCTC CTACTCTCCC ACGTTCTAAC ACAGTGGCTT CTTTCTCCTC CCTGTACCAG
    CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCGGC CGTGGTTCTT
    GAGGAGGGAA GTCCAGGAGA GGCTCATGTG CCAGTGGAGC CCCCCGAATT GGAGGACTTT
    GAGGCAGCAT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACGCCTACAG CAGGTCTTCC
    TCAGCATCTA GCCAGGAAGA AAAGTCCCAC TTGGAGGAGC TGGCTGCAGG GGGTATCCCC
    ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTACAG TGGACCTGTC CTTCCAACCC
    TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCTGAGTTAC AGACCTTACA GGACATCCTT
    GGAGACCTAG GGGACAAGAC TGACATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGATCC
    CAGTCGGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTCCGGGTGA AGAGAGCCGT
    ATCACAGTCC CACCTGAAGG TCCCCTGCCT TCCAGTTCTC CCCGCTCCCC TAGTGGCCTC
    CGGCCCCGAG GCTATACCAT CTCTGATTCA GCTCCATCAC GAAGGGGAAA GAGGGTAGAA
    AGGGACAACT TCAAGAGCAG AACTGCGGCC TCCAGTGCTG AAAAGGTGCC AGGCATCAAC
    CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCACCCTTTT TTGGTGATGA GTCCAATAAG
    CCCATCCTGT TGCCCAACGA GTCCTTTGAA CGGTCAGTGC AGCTCCTTGA CCAGATCCCA
    TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG
    CTGGCCATCC TATCAAATGA GCATGGCTCT TATAGGTACA CAGAGTTTCT GACAGGCCTG
    GGTCGGCTTA TTGAGCTGAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT
    GTGTGTGGCG AGGATGGACA GTTCACGTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT
    TTCCACATTG CTACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGTTG TGACAAGAAA
    CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAG
    CTGGGCACCA TTAAGGGCCA GTTCAACTTC GTCCATGTGA TCATCACACC GCTGGACTAT
    AAATGCAACC TGTTGACCCT GCAGTGCAGG AAAGACATGG AGGGCCTTGT GGACACCAGT
    GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT
    GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACGGACAT CTATCCCTCC
    AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGTC AGCGGATCCG TGAAGAAGTG
    CACTATTCAA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCATACCAA AGTCCCAGCT
    CAAGCCCCTA CGGAGGCTAC ACCCACCTAC GAGACAGGCC AGCGGAAACG CCTCATTTCC
    TCTGTGGATG ACTTCACAGA GTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006245971.2
    CDS198..5627
    Translation

    Target ORF information:

    RefSeq Version XM_006245909.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006245909.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT
    CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CTCTTACCAG TGCCAGCTTG
    GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACCTGAAGAA TCTCCACTTG
    GAACTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA CTTCACTGCG
    GTTCCCAAGA GGTCCCCTGT GGGAGAATTC CTCCTGGCAG GGGGCAGGAC CAAAACGTGG
    CTGGTTGGAA ATAAGCTGGT CACTGTGACA ACGAGTGTGG GAACTGGTAC GCGGTCGTTG
    CTGGGCCTGG ACTCCGGGGA TCTGCAGGGT GGCTCAGCAT CAAGCTCTGA TCCTGGCACA
    CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG AGTCCGCTCC ATGTCAGGGG GCCACGGCCT TCGAGTTGGT
    GTCCTGGACA CTTCAGCTCC CTACACCCCA GGAGGCCCTG CTTCTCTGGG AGCACAGGCA
    GCACCAGCAG CAAGGCCTGA GAAGCCCTGT GCAGGAGCCC AACTTCCGGC AGCGGAGAAG
    GCGAACCTGG CAGCCTATGT GCCTTTGCTG ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC
    AGGCCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTTTCC
    TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCTAATG CCCTTATGGC TGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACA
    GCCAAGCCTC CTACTCTCCC ACGTTCTAAC ACAGTGGCTT CTTTCTCCTC CCTGTACCAG
    CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCGGC CGTGGTTCTT
    GAGGAGGGAA GTCCAGGAGA GGCTCATGTG CCAGTGGAGC CCCCCGAATT GGAGGACTTT
    GAGGCAGCAT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACGCCTACAG CAGGTCTTCC
    TCAGCATCTA GCCAGGAAGA AAAGTCCCAC TTGGAGGAGC TGGCTGCAGG GGGTATCCCC
    ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTACAG TGGACCTGTC CTTCCAACCC
    TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCTGAGTTAC AGACCTTACA GGACATCCTT
    GGAGACCTAG GGGACAAGAC TGACATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGATCC
    CAGTCGGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTCCGGGTGA AGAGAGCCGT
    ATCACAGTCC CACCTGAAGG TCCCCTGCCT TCCAGTTCTC CCCGCTCCCC TAGTGGCCTC
    CGGCCCCGAG GCTATACCAT CTCTGATTCA GCTCCATCAC GAAGGGGAAA GAGGGTAGAA
    AGGGACAACT TCAAGAGCAG AACTGCGGCC TCCAGTGCTG AAAAGGTGCC AGGCATCAAC
    CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCACCCTTTT TTGGTGATGA GTCCAATAAG
    CCCATCCTGT TGCCCAACGA GTCCTTTGAA CGGTCAGTGC AGCTCCTTGA CCAGATCCCA
    TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG
    CTGGCCATCC TATCAAATGA GCATGGCTCT TATAGGTACA CAGAGTTTCT GACAGGCCTG
    GGTCGGCTTA TTGAGCTGAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT
    GTGTGTGGCG AGGATGGACA GTTCACGTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT
    TTCCACATTG CTACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGTTG TGACAAGAAA
    CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAG
    CTGGGCACCA TTAAGGGCCA GTTCAACTTC GTCCATGTGA TCATCACACC GCTGGACTAT
    AAATGCAACC TGTTGACCCT GCAGTGCAGG AAAGACATGG AGGGCCTTGT GGACACCAGT
    GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT
    GCAAATATGG CCTCACAGGT ACACCACAGC CGATCCAACC CCACGGACAT CTATCCCTCC
    AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGTC AGCGGATCCG TGAAGAAGTG
    CACTATTCAA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCATACCAA AGTCCCAGCT
    CAAGCCCCTA CGGAGGCTAC ACCCACCTAC GAGACAGGCC AGCGGAAACG CCTCATTTCC
    TCTGTGGATG ACTTCACAGA GTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57181
    Clone ID Related Accession (Same CDS sequence) XM_017597019.1
    Accession Version XM_017597019.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT
    CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCTC TTACCAGTGC CAGCTTGGGG
    TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACC TGAAGAATCT CCACTTGGAA
    CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAACTT CACTGCGGTT
    CCCAAGAGGT CCCCTGTGGG AGAATTCCTC CTGGCAGGGG GCAGGACCAA AACGTGGCTG
    GTTGGAAATA AGCTGGTCAC TGTGACAACG AGTGTGGGAA CTGGTACGCG GTCGTTGCTG
    GGCCTGGACT CCGGGGATCT GCAGGGTGGC TCAGCATCAA GCTCTGATCC TGGCACACAT
    GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC
    CGTGGGGCCC GGGACCGAGT CCGCTCCATG TCAGGGGGCC ACGGCCTTCG AGTTGGTGTC
    CTGGACACTT CAGCTCCCTA CACCCCAGGA GGCCCTGCTT CTCTGGGAGC ACAGGCAGCA
    CCAGCAGCAA GGCCTGAGAA GCCCTGTGCA GGAGCCCAAC TTCCGGCAGC GGAGAAGGCG
    AACCTGGCAG CCTATGTGCC TTTGCTGACC CAGGGCTGGG CAGAGATCCT GGTCCGCAGG
    CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTTTCCTCA
    GACATCAACA ACATGCCCCT GCAAGAGCTG TCTAATGCCC TTATGGCTGC TGAGCGCTTC
    AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACAGCC
    AAGCCTCCTA CTCTCCCACG TTCTAACACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC
    AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCGGCCGT GGTTCTTGAG
    GAGGGAAGTC CAGGAGAGGC TCATGTGCCA GTGGAGCCCC CCGAATTGGA GGACTTTGAG
    GCAGCATTAG GTACAGACAG GCACTGCCAG CGGCCTGACG CCTACAGCAG GTCTTCCTCA
    GCATCTAGCC AGGAAGAAAA GTCCCACTTG GAGGAGCTGG CTGCAGGGGG TATCCCCATT
    GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTACAGTGG ACCTGTCCTT CCAACCCTCA
    CAGCCTTTGA GCAAGTCTAG CTCTTCTCCT GAGTTACAGA CCTTACAGGA CATCCTTGGA
    GACCTAGGGG ACAAGACTGA CATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGATCCCAG
    TCGGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTC CGGGTGAAGA GAGCCGTATC
    ACAGTCCCAC CTGAAGGTCC CCTGCCTTCC AGTTCTCCCC GCTCCCCTAG TGGCCTCCGG
    CCCCGAGGCT ATACCATCTC TGATTCAGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG
    GACAACTTCA AGAGCAGAAC TGCGGCCTCC AGTGCTGAAA AGGTGCCAGG CATCAACCCT
    AGCTTTGTGT TCCTACAGCT CTACCATTCA CCCTTTTTTG GTGATGAGTC CAATAAGCCC
    ATCCTGTTGC CCAACGAGTC CTTTGAACGG TCAGTGCAGC TCCTTGACCA GATCCCATCC
    TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG
    GCCATCCTAT CAAATGAGCA TGGCTCTTAT AGGTACACAG AGTTTCTGAC AGGCCTGGGT
    CGGCTTATTG AGCTGAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTG
    TGTGGCGAGG ATGGACAGTT CACGTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGTTGTGA CAAGAAACGG
    CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAGCTG
    GGCACCATTA AGGGCCAGTT CAACTTCGTC CATGTGATCA TCACACCGCT GGACTATAAA
    TGCAACCTGT TGACCCTGCA GTGCAGGAAA GACATGGAGG GCCTTGTGGA CACCAGTGTG
    GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA
    AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CGGACATCTA TCCCTCCAAG
    TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGTCAGC GGATCCGTGA AGAAGTGCAC
    TATTCAAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ATACCAAAGT CCCAGCTCAA
    GCCCCTACGG AGGCTACACC CACCTACGAG ACAGGCCAGC GGAAACGCCT CATTTCCTCT
    GTGGATGACT TCACAGAGTT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452508.1
    CDS203..5629
    Translation

    Target ORF information:

    RefSeq Version XM_017597019.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT
    CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTATCTG AACATGTGGT CCGCAGAATA CAGACATCTC TTACCAGTGC CAGCTTGGGG
    TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACC TGAAGAATCT CCACTTGGAA
    CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAACTT CACTGCGGTT
    CCCAAGAGGT CCCCTGTGGG AGAATTCCTC CTGGCAGGGG GCAGGACCAA AACGTGGCTG
    GTTGGAAATA AGCTGGTCAC TGTGACAACG AGTGTGGGAA CTGGTACGCG GTCGTTGCTG
    GGCCTGGACT CCGGGGATCT GCAGGGTGGC TCAGCATCAA GCTCTGATCC TGGCACACAT
    GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC
    CGTGGGGCCC GGGACCGAGT CCGCTCCATG TCAGGGGGCC ACGGCCTTCG AGTTGGTGTC
    CTGGACACTT CAGCTCCCTA CACCCCAGGA GGCCCTGCTT CTCTGGGAGC ACAGGCAGCA
    CCAGCAGCAA GGCCTGAGAA GCCCTGTGCA GGAGCCCAAC TTCCGGCAGC GGAGAAGGCG
    AACCTGGCAG CCTATGTGCC TTTGCTGACC CAGGGCTGGG CAGAGATCCT GGTCCGCAGG
    CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTTTCCTCA
    GACATCAACA ACATGCCCCT GCAAGAGCTG TCTAATGCCC TTATGGCTGC TGAGCGCTTC
    AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACAGCC
    AAGCCTCCTA CTCTCCCACG TTCTAACACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC
    AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCGGCCGT GGTTCTTGAG
    GAGGGAAGTC CAGGAGAGGC TCATGTGCCA GTGGAGCCCC CCGAATTGGA GGACTTTGAG
    GCAGCATTAG GTACAGACAG GCACTGCCAG CGGCCTGACG CCTACAGCAG GTCTTCCTCA
    GCATCTAGCC AGGAAGAAAA GTCCCACTTG GAGGAGCTGG CTGCAGGGGG TATCCCCATT
    GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTACAGTGG ACCTGTCCTT CCAACCCTCA
    CAGCCTTTGA GCAAGTCTAG CTCTTCTCCT GAGTTACAGA CCTTACAGGA CATCCTTGGA
    GACCTAGGGG ACAAGACTGA CATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGATCCCAG
    TCGGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTC CGGGTGAAGA GAGCCGTATC
    ACAGTCCCAC CTGAAGGTCC CCTGCCTTCC AGTTCTCCCC GCTCCCCTAG TGGCCTCCGG
    CCCCGAGGCT ATACCATCTC TGATTCAGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG
    GACAACTTCA AGAGCAGAAC TGCGGCCTCC AGTGCTGAAA AGGTGCCAGG CATCAACCCT
    AGCTTTGTGT TCCTACAGCT CTACCATTCA CCCTTTTTTG GTGATGAGTC CAATAAGCCC
    ATCCTGTTGC CCAACGAGTC CTTTGAACGG TCAGTGCAGC TCCTTGACCA GATCCCATCC
    TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG
    GCCATCCTAT CAAATGAGCA TGGCTCTTAT AGGTACACAG AGTTTCTGAC AGGCCTGGGT
    CGGCTTATTG AGCTGAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTG
    TGTGGCGAGG ATGGACAGTT CACGTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGTTGTGA CAAGAAACGG
    CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAGCTG
    GGCACCATTA AGGGCCAGTT CAACTTCGTC CATGTGATCA TCACACCGCT GGACTATAAA
    TGCAACCTGT TGACCCTGCA GTGCAGGAAA GACATGGAGG GCCTTGTGGA CACCAGTGTG
    GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA
    AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CGGACATCTA TCCCTCCAAG
    TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGTCAGC GGATCCGTGA AGAAGTGCAC
    TATTCAAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ATACCAAAGT CCCAGCTCAA
    GCCCCTACGG AGGCTACACC CACCTACGAG ACAGGCCAGC GGAAACGCCT CATTTCCTCT
    GTGGATGACT TCACAGAGTT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57182
    Clone ID Related Accession (Same CDS sequence) XM_017597020.1
    Accession Version XM_017597020.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTCGTG
    AACCTGGTCA AGTTCAACAG CTGCTACCTT GACGAATACA TTGCGCCAAT GGTTCACATG
    ATTTGTCTGT TATGTATCCG GACAGTGTCC TCTGTGGACA TCGAGGTGTC CTTGCAAGTG
    CTGGATGCTG TGGTCTGCTA CAACTGCCTG CCTGCTGAGA GCCTGCCTCT GTTCATTATC
    ACCCTGTGCC GCACCGTCAA CGTCAAGGAG CTGTGTGAGC CGTGCTGGAA GCTGATGCGA
    AACCTTCTAG GTACCCATCT AGGCCACAGT GCCATTTACA ACATGTGCCG TATCATGGAG
    AACAGATCCT ACATGGAAGA CGCCCCCTTG TTGAGGGGAG CCGTGTTCTT TGTTGGGATG
    GCGCTCTGGG GAGCTCACCG ACTCTATTCG CTCAAGAACT CCCCCACATC TGTGCTGCCG
    TCTTTTTATG AGGCCATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC
    ATAACAAGAC TCATCAAGAA GTACAGGAAG GAGCTCCAGG CTGTGACATG GGACATTCTG
    CTGGACATCA TTGAACGACT GCTCCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAGG
    ACCATTGTCC ATGACCTGCT GACCACTGTG GAGGAGCTGT GTGACCAGAA CGAGTTCCAC
    GGATCGCAAG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG GCCTGAATCC
    TCTCTCTTAA ACCTGATAAC CTACAGAGCC CAGTCTATCC ACCCAGCCAA GGATGGCTGG
    ATCCAGAACC TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ACGAGTGCCG CAGTGCCGTG
    CGCATCAAGG TGCTGGACGT GCTGTCCTTC GTGCTCCTCA TCAACAGGCA GTTCTACGAG
    GAGGAGCTGA TTAACTCGGT GGTCATCTCA CAGCTCTCCC ACATCCCCGA GGATAAAGAC
    CATCAGGTTC GAAAGCTGGC TACCCAGCTG CTGGTGGACC TGGCAGAGGG CTGCCACACC
    CACCACTTCA ACAGTCTGTT GGACATCATT GAAAAGGTGA TGGCTCGCTC CCTTTCTCCA
    CCCCTGGAGC TGGAGGAAAG GGATCTGGCT GTGTACTCGG CCTCCCTGGA GGATGTGAAG
    ACGGCAGTCC TCGGGCTCCT GGTCATCCTT CAGACCAAGC TGTATACCTT GCCCGCCAGC
    CATGCCACAC GAGTGTATGA GACACTCATT AGCCACATCC AGCTCCATTA CAAGCACGGC
    TACTCCTTGC CCATTGCTAG CAGCATCCGG CTGCAGGCCT TTGACTTCCT GCTGCTACTG
    CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GCGATTCAGC
    CCTTACTGCC TCTGTGACTG CGCGGAACTG GACAGGGCCT CAGAGAAGAA AGCCAGCGGA
    CCCCTTTCAC CGCCAACTGG GCCCCCCAGC CCTGTGCCAA CGGGCCCTGC TGTGAGGCTC
    GGCCACCTGC CCTACTCCCT GCTTTTCCGT GTCCTGCTGC AGTGTTTGAA GCAGGAGACC
    GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AAACTCCCTG AGTCGCTGCG CTACAAAGTC
    CTCATTTTCA CCTCCCCCTG CAGTGTTGAC CAGCTGTCTT CTGCTCTCTG CTCCATGCTT
    TCAGCCCCGA AGACCCTTGA GCGGCTCCGA GGCACCCCAG AAGGCTTCTC CAGAACTGAC
    CTGCACTTGG CTGTGGTCCC TGTACTGACA GCGTTAATAT CTTACCACAA CTATTTGGAT
    AAGACTAGAC AGCGTGAGAT GGTTTACTGC CTAGAACAAG GCCTTATCTA CCGCTGTGCC
    AGCCAGTGTG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG
    GCGCTCCCTG TCCTGGTGGT GAAGCTGACA CACATCTCTG CTACAGCTAG CATGGCCATC
    CCCCTCCTGG AGTTCCTGTC TACCTTGGCC AGGCTGCCTC ACCTCTACAG GAACTTCGCC
    GCAGAGCAGT ATGCAAGTGT GTTTGCCATC TCCTTGCCCT ACACCAACCC CTCCAAGTTC
    AACCAGTACA TTGTGTGTCT GGCCCATCAC GTCATAGCCA TGTGGTTCAT TAGGTGCCGA
    CTGCCCTTTC GGAAGGATTT TGTCCCTTAT ATCACTAAGG GTCTGCGTTC CAATGTCCTC
    CTGTCCTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT
    GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA
    CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT
    GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCTC TTACCAGTGC CAGCTTGGGG
    TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACC TGAAGAATCT CCACTTGGAA
    CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAACTT CACTGCGGTT
    CCCAAGAGGT CCCCTGTGGG AGAATTCCTC CTGGCAGGGG GCAGGACCAA AACGTGGCTG
    GTTGGAAATA AGCTGGTCAC TGTGACAACG AGTGTGGGAA CTGGTACGCG GTCGTTGCTG
    GGCCTGGACT CCGGGGATCT GCAGGGTGGC TCAGCATCAA GCTCTGATCC TGGCACACAT
    GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC
    CGTGGGGCCC GGGACCGAGT CCGCTCCATG TCAGGGGGCC ACGGCCTTCG AGTTGGTGTC
    CTGGACACTT CAGCTCCCTA CACCCCAGGA GGCCCTGCTT CTCTGGGAGC ACAGGCAGCA
    CCAGCAGCAA GGCCTGAGAA GCCCTGTGCA GGAGCCCAAC TTCCGGCAGC GGAGAAGGCG
    AACCTGGCAG CCTATGTGCC TTTGCTGACC CAGGGCTGGG CAGAGATCCT GGTCCGCAGG
    CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTTTCCTCA
    GACATCAACA ACATGCCCCT GCAAGAGCTG TCTAATGCCC TTATGGCTGC TGAGCGCTTC
    AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACAGCC
    AAGCCTCCTA CTCTCCCACG TTCTAACACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC
    AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCGGCCGT GGTTCTTGAG
    GAGGGAAGTC CAGGAGAGGC TCATGTGCCA GTGGAGCCCC CCGAATTGGA GGACTTTGAG
    GCAGCATTAG GTACAGACAG GCACTGCCAG CGGCCTGACG CCTACAGCAG GTCTTCCTCA
    GCATCTAGCC AGGAAGAAAA GTCCCACTTG GAGGAGCTGG CTGCAGGGGG TATCCCCATT
    GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTACAGTGG ACCTGTCCTT CCAACCCTCA
    CAGCCTTTGA GCAAGTCTAG CTCTTCTCCT GAGTTACAGA CCTTACAGGA CATCCTTGGA
    GACCTAGGGG ACAAGACTGA CATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGATCCCAG
    TCGGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTC CGGGTGAAGA GAGCCGTATC
    ACAGTCCCAC CTGAAGGTCC CCTGCCTTCC AGTTCTCCCC GCTCCCCTAG TGGCCTCCGG
    CCCCGAGGCT ATACCATCTC TGATTCAGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG
    GACAACTTCA AGAGCAGAAC TGCGGCCTCC AGTGCTGAAA AGGTGCCAGG CATCAACCCT
    AGCTTTGTGT TCCTACAGCT CTACCATTCA CCCTTTTTTG GTGATGAGTC CAATAAGCCC
    ATCCTGTTGC CCAACGAGTC CTTTGAACGG TCAGTGCAGC TCCTTGACCA GATCCCATCC
    TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG
    GCCATCCTAT CAAATGAGCA TGGCTCTTAT AGGTACACAG AGTTTCTGAC AGGCCTGGGT
    CGGCTTATTG AGCTGAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTG
    TGTGGCGAGG ATGGACAGTT CACGTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGTTGTGA CAAGAAACGG
    CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAGCTG
    GGCACCATTA AGGGCCAGTT CAACTTCGTC CATGTGATCA TCACACCGCT GGACTATAAA
    TGCAACCTGT TGACCCTGCA GTGCAGGAAA GACATGGAGG GCCTTGTGGA CACCAGTGTG
    GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA
    AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CGGACATCTA TCCCTCCAAG
    TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGTCAGC GGATCCGTGA AGAAGTGCAC
    TATTCAAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ATACCAAAGT CCCAGCTCAA
    GCCCCTACGG AGGCTACACC CACCTACGAG ACAGGCCAGC GGAAACGCCT CATTTCCTCT
    GTGGATGACT TCACAGAGTT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452509.1
    CDS203..5629
    Translation

    Target ORF information:

    RefSeq Version XM_017597020.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597020.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTCGTG
    AACCTGGTCA AGTTCAACAG CTGCTACCTT GACGAATACA TTGCGCCAAT GGTTCACATG
    ATTTGTCTGT TATGTATCCG GACAGTGTCC TCTGTGGACA TCGAGGTGTC CTTGCAAGTG
    CTGGATGCTG TGGTCTGCTA CAACTGCCTG CCTGCTGAGA GCCTGCCTCT GTTCATTATC
    ACCCTGTGCC GCACCGTCAA CGTCAAGGAG CTGTGTGAGC CGTGCTGGAA GCTGATGCGA
    AACCTTCTAG GTACCCATCT AGGCCACAGT GCCATTTACA ACATGTGCCG TATCATGGAG
    AACAGATCCT ACATGGAAGA CGCCCCCTTG TTGAGGGGAG CCGTGTTCTT TGTTGGGATG
    GCGCTCTGGG GAGCTCACCG ACTCTATTCG CTCAAGAACT CCCCCACATC TGTGCTGCCG
    TCTTTTTATG AGGCCATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC
    ATAACAAGAC TCATCAAGAA GTACAGGAAG GAGCTCCAGG CTGTGACATG GGACATTCTG
    CTGGACATCA TTGAACGACT GCTCCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAGG
    ACCATTGTCC ATGACCTGCT GACCACTGTG GAGGAGCTGT GTGACCAGAA CGAGTTCCAC
    GGATCGCAAG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG GCCTGAATCC
    TCTCTCTTAA ACCTGATAAC CTACAGAGCC CAGTCTATCC ACCCAGCCAA GGATGGCTGG
    ATCCAGAACC TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ACGAGTGCCG CAGTGCCGTG
    CGCATCAAGG TGCTGGACGT GCTGTCCTTC GTGCTCCTCA TCAACAGGCA GTTCTACGAG
    GAGGAGCTGA TTAACTCGGT GGTCATCTCA CAGCTCTCCC ACATCCCCGA GGATAAAGAC
    CATCAGGTTC GAAAGCTGGC TACCCAGCTG CTGGTGGACC TGGCAGAGGG CTGCCACACC
    CACCACTTCA ACAGTCTGTT GGACATCATT GAAAAGGTGA TGGCTCGCTC CCTTTCTCCA
    CCCCTGGAGC TGGAGGAAAG GGATCTGGCT GTGTACTCGG CCTCCCTGGA GGATGTGAAG
    ACGGCAGTCC TCGGGCTCCT GGTCATCCTT CAGACCAAGC TGTATACCTT GCCCGCCAGC
    CATGCCACAC GAGTGTATGA GACACTCATT AGCCACATCC AGCTCCATTA CAAGCACGGC
    TACTCCTTGC CCATTGCTAG CAGCATCCGG CTGCAGGCCT TTGACTTCCT GCTGCTACTG
    CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GCGATTCAGC
    CCTTACTGCC TCTGTGACTG CGCGGAACTG GACAGGGCCT CAGAGAAGAA AGCCAGCGGA
    CCCCTTTCAC CGCCAACTGG GCCCCCCAGC CCTGTGCCAA CGGGCCCTGC TGTGAGGCTC
    GGCCACCTGC CCTACTCCCT GCTTTTCCGT GTCCTGCTGC AGTGTTTGAA GCAGGAGACC
    GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AAACTCCCTG AGTCGCTGCG CTACAAAGTC
    CTCATTTTCA CCTCCCCCTG CAGTGTTGAC CAGCTGTCTT CTGCTCTCTG CTCCATGCTT
    TCAGCCCCGA AGACCCTTGA GCGGCTCCGA GGCACCCCAG AAGGCTTCTC CAGAACTGAC
    CTGCACTTGG CTGTGGTCCC TGTACTGACA GCGTTAATAT CTTACCACAA CTATTTGGAT
    AAGACTAGAC AGCGTGAGAT GGTTTACTGC CTAGAACAAG GCCTTATCTA CCGCTGTGCC
    AGCCAGTGTG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG
    GCGCTCCCTG TCCTGGTGGT GAAGCTGACA CACATCTCTG CTACAGCTAG CATGGCCATC
    CCCCTCCTGG AGTTCCTGTC TACCTTGGCC AGGCTGCCTC ACCTCTACAG GAACTTCGCC
    GCAGAGCAGT ATGCAAGTGT GTTTGCCATC TCCTTGCCCT ACACCAACCC CTCCAAGTTC
    AACCAGTACA TTGTGTGTCT GGCCCATCAC GTCATAGCCA TGTGGTTCAT TAGGTGCCGA
    CTGCCCTTTC GGAAGGATTT TGTCCCTTAT ATCACTAAGG GTCTGCGTTC CAATGTCCTC
    CTGTCCTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT
    GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA
    CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT
    GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCTC TTACCAGTGC CAGCTTGGGG
    TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACC TGAAGAATCT CCACTTGGAA
    CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAACTT CACTGCGGTT
    CCCAAGAGGT CCCCTGTGGG AGAATTCCTC CTGGCAGGGG GCAGGACCAA AACGTGGCTG
    GTTGGAAATA AGCTGGTCAC TGTGACAACG AGTGTGGGAA CTGGTACGCG GTCGTTGCTG
    GGCCTGGACT CCGGGGATCT GCAGGGTGGC TCAGCATCAA GCTCTGATCC TGGCACACAT
    GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC
    CGTGGGGCCC GGGACCGAGT CCGCTCCATG TCAGGGGGCC ACGGCCTTCG AGTTGGTGTC
    CTGGACACTT CAGCTCCCTA CACCCCAGGA GGCCCTGCTT CTCTGGGAGC ACAGGCAGCA
    CCAGCAGCAA GGCCTGAGAA GCCCTGTGCA GGAGCCCAAC TTCCGGCAGC GGAGAAGGCG
    AACCTGGCAG CCTATGTGCC TTTGCTGACC CAGGGCTGGG CAGAGATCCT GGTCCGCAGG
    CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTTTCCTCA
    GACATCAACA ACATGCCCCT GCAAGAGCTG TCTAATGCCC TTATGGCTGC TGAGCGCTTC
    AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACAGCC
    AAGCCTCCTA CTCTCCCACG TTCTAACACA GTGGCTTCTT TCTCCTCCCT GTACCAGCCC
    AGCTGCCAAG GACAGCTGCA CAGGAGCGTT TCCTGGGCAG ACTCGGCCGT GGTTCTTGAG
    GAGGGAAGTC CAGGAGAGGC TCATGTGCCA GTGGAGCCCC CCGAATTGGA GGACTTTGAG
    GCAGCATTAG GTACAGACAG GCACTGCCAG CGGCCTGACG CCTACAGCAG GTCTTCCTCA
    GCATCTAGCC AGGAAGAAAA GTCCCACTTG GAGGAGCTGG CTGCAGGGGG TATCCCCATT
    GAGCGGGCCA TCTCCTCTGA GGGGGCTCGG CCTACAGTGG ACCTGTCCTT CCAACCCTCA
    CAGCCTTTGA GCAAGTCTAG CTCTTCTCCT GAGTTACAGA CCTTACAGGA CATCCTTGGA
    GACCTAGGGG ACAAGACTGA CATTGGACGG CTGAGTCCTG AGGCTAAGGT CCGATCCCAG
    TCGGGGATCC TGGATGGGGA AGCAGCTACC TGGTCAGCTC CGGGTGAAGA GAGCCGTATC
    ACAGTCCCAC CTGAAGGTCC CCTGCCTTCC AGTTCTCCCC GCTCCCCTAG TGGCCTCCGG
    CCCCGAGGCT ATACCATCTC TGATTCAGCT CCATCACGAA GGGGAAAGAG GGTAGAAAGG
    GACAACTTCA AGAGCAGAAC TGCGGCCTCC AGTGCTGAAA AGGTGCCAGG CATCAACCCT
    AGCTTTGTGT TCCTACAGCT CTACCATTCA CCCTTTTTTG GTGATGAGTC CAATAAGCCC
    ATCCTGTTGC CCAACGAGTC CTTTGAACGG TCAGTGCAGC TCCTTGACCA GATCCCATCC
    TATGACACTC ACAAGATTGC TGTCCTGTAT GTGGGAGAAG GCCAGAGCAG CAGTGAGCTG
    GCCATCCTAT CAAATGAGCA TGGCTCTTAT AGGTACACAG AGTTTCTGAC AGGCCTGGGT
    CGGCTTATTG AGCTGAAGGA CTGCCAACCA GACAAGGTGT ACTTAGGTGG ACTGGATGTG
    TGTGGCGAGG ATGGACAGTT CACGTACTGC TGGCATGATG ACATCATGCA AGCTGTTTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAT GTGGACAAGC ACCGTTGTGA CAAGAAACGG
    CACCTGGGCA ATGACTTTGT TTCTATCATC TACAATGATT CTGGTGAGGA CTTCAAGCTG
    GGCACCATTA AGGGCCAGTT CAACTTCGTC CATGTGATCA TCACACCGCT GGACTATAAA
    TGCAACCTGT TGACCCTGCA GTGCAGGAAA GACATGGAGG GCCTTGTGGA CACCAGTGTG
    GCCAAGATTG TGTCTGACCG CAACCTGTCC TTTGTGGCCC GACAGATGGC CCTGCATGCA
    AATATGGCCT CACAGGTACA CCACAGCCGA TCCAACCCCA CGGACATCTA TCCCTCCAAG
    TGGATCGCAA GACTCCGCCA CATTAAGCGT CTCCGTCAGC GGATCCGTGA AGAAGTGCAC
    TATTCAAACC CAAGCTTGCC TCTGATGCAC CCTCCAGCCC ATACCAAAGT CCCAGCTCAA
    GCCCCTACGG AGGCTACACC CACCTACGAG ACAGGCCAGC GGAAACGCCT CATTTCCTCT
    GTGGATGACT TCACAGAGTT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57183
    Clone ID Related Accession (Same CDS sequence) XM_017597021.1
    Accession Version XM_017597021.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5361bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT
    CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CTCTTACCAG TGCCAGCTTG
    GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACCTGAAGAA TCTCCACTTG
    GAACTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA CTTCACTGCG
    GTTCCCAAGA GGTCCCCTGT GGGAGAATTC CTCCTGGCAG GGGGCAGGAC CAAAACGTGG
    CTGGTTGGAA ATAAGCTGGT CACTGTGACA ACGAGTGTGG GAACTGGTAC GCGGTCGTTG
    CTGGGCCTGG ACTCCGGGGA TCTGCAGGGT GGCTCAGCAT CAAGCTCTGA TCCTGGCACA
    CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG AGTCCGCTCC ATGTCAGGGG GCCACGGCCT TCGAGTTGGT
    GTCCTGGACA CTTCAGCTCC CTACACCCCA GGAGGCCCTG CTTCTCTGGG AGCACAGGCA
    GCACCAGCAG CAAGGCCTGA GAAGCCCTGT GCAGGAGCCC AACTTCCGGC AGCGGAGAAG
    GCGAACCTGG CAGCCTATGT GCCTTTGCTG ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC
    AGGCCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTTTCC
    TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCTAATG CCCTTATGGC TGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACA
    GCCAAGCCTC CTACTCTCCC ACGTTCTAAC ACAGACTCGG CCGTGGTTCT TGAGGAGGGA
    AGTCCAGGAG AGGCTCATGT GCCAGTGGAG CCCCCCGAAT TGGAGGACTT TGAGGCAGCA
    TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACGCCTACA GCAGGTCTTC CTCAGCATCT
    AGCCAGGAAG AAAAGTCCCA CTTGGAGGAG CTGGCTGCAG GGGGTATCCC CATTGAGCGG
    GCCATCTCCT CTGAGGGGGC TCGGCCTACA GTGGACCTGT CCTTCCAACC CTCACAGCCT
    TTGAGCAAGT CTAGCTCTTC TCCTGAGTTA CAGACCTTAC AGGACATCCT TGGAGACCTA
    GGGGACAAGA CTGACATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGATC CCAGTCGGGG
    ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTCCGGGTG AAGAGAGCCG TATCACAGTC
    CCACCTGAAG GTCCCCTGCC TTCCAGTTCT CCCCGCTCCC CTAGTGGCCT CCGGCCCCGA
    GGCTATACCA TCTCTGATTC AGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGACAAC
    TTCAAGAGCA GAACTGCGGC CTCCAGTGCT GAAAAGGTGC CAGGCATCAA CCCTAGCTTT
    GTGTTCCTAC AGCTCTACCA TTCACCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTG
    TTGCCCAACG AGTCCTTTGA ACGGTCAGTG CAGCTCCTTG ACCAGATCCC ATCCTATGAC
    ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC
    CTATCAAATG AGCATGGCTC TTATAGGTAC ACAGAGTTTC TGACAGGCCT GGGTCGGCTT
    ATTGAGCTGA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTGTGTGGC
    GAGGATGGAC AGTTCACGTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT
    GCTACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGTT GTGACAAGAA ACGGCACCTG
    GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA GCTGGGCACC
    ATTAAGGGCC AGTTCAACTT CGTCCATGTG ATCATCACAC CGCTGGACTA TAAATGCAAC
    CTGTTGACCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG TGTGGCCAAG
    ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG
    GCCTCACAGG TACACCACAG CCGATCCAAC CCCACGGACA TCTATCCCTC CAAGTGGATC
    GCAAGACTCC GCCACATTAA GCGTCTCCGT CAGCGGATCC GTGAAGAAGT GCACTATTCA
    AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCATACCA AAGTCCCAGC TCAAGCCCCT
    ACGGAGGCTA CACCCACCTA CGAGACAGGC CAGCGGAAAC GCCTCATTTC CTCTGTGGAT
    GACTTCACAG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452510.1
    CDS202..5562
    Translation

    Target ORF information:

    RefSeq Version XM_017597021.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT
    CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CTCTTACCAG TGCCAGCTTG
    GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACCTGAAGAA TCTCCACTTG
    GAACTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA CTTCACTGCG
    GTTCCCAAGA GGTCCCCTGT GGGAGAATTC CTCCTGGCAG GGGGCAGGAC CAAAACGTGG
    CTGGTTGGAA ATAAGCTGGT CACTGTGACA ACGAGTGTGG GAACTGGTAC GCGGTCGTTG
    CTGGGCCTGG ACTCCGGGGA TCTGCAGGGT GGCTCAGCAT CAAGCTCTGA TCCTGGCACA
    CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG AGTCCGCTCC ATGTCAGGGG GCCACGGCCT TCGAGTTGGT
    GTCCTGGACA CTTCAGCTCC CTACACCCCA GGAGGCCCTG CTTCTCTGGG AGCACAGGCA
    GCACCAGCAG CAAGGCCTGA GAAGCCCTGT GCAGGAGCCC AACTTCCGGC AGCGGAGAAG
    GCGAACCTGG CAGCCTATGT GCCTTTGCTG ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC
    AGGCCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTTTCC
    TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCTAATG CCCTTATGGC TGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC GGCCCTGTAC AAGTCATTGT CAGTGCCAGC GGCTGGCACA
    GCCAAGCCTC CTACTCTCCC ACGTTCTAAC ACAGACTCGG CCGTGGTTCT TGAGGAGGGA
    AGTCCAGGAG AGGCTCATGT GCCAGTGGAG CCCCCCGAAT TGGAGGACTT TGAGGCAGCA
    TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACGCCTACA GCAGGTCTTC CTCAGCATCT
    AGCCAGGAAG AAAAGTCCCA CTTGGAGGAG CTGGCTGCAG GGGGTATCCC CATTGAGCGG
    GCCATCTCCT CTGAGGGGGC TCGGCCTACA GTGGACCTGT CCTTCCAACC CTCACAGCCT
    TTGAGCAAGT CTAGCTCTTC TCCTGAGTTA CAGACCTTAC AGGACATCCT TGGAGACCTA
    GGGGACAAGA CTGACATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGATC CCAGTCGGGG
    ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTCCGGGTG AAGAGAGCCG TATCACAGTC
    CCACCTGAAG GTCCCCTGCC TTCCAGTTCT CCCCGCTCCC CTAGTGGCCT CCGGCCCCGA
    GGCTATACCA TCTCTGATTC AGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGACAAC
    TTCAAGAGCA GAACTGCGGC CTCCAGTGCT GAAAAGGTGC CAGGCATCAA CCCTAGCTTT
    GTGTTCCTAC AGCTCTACCA TTCACCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTG
    TTGCCCAACG AGTCCTTTGA ACGGTCAGTG CAGCTCCTTG ACCAGATCCC ATCCTATGAC
    ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC
    CTATCAAATG AGCATGGCTC TTATAGGTAC ACAGAGTTTC TGACAGGCCT GGGTCGGCTT
    ATTGAGCTGA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTGTGTGGC
    GAGGATGGAC AGTTCACGTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT
    GCTACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGTT GTGACAAGAA ACGGCACCTG
    GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA GCTGGGCACC
    ATTAAGGGCC AGTTCAACTT CGTCCATGTG ATCATCACAC CGCTGGACTA TAAATGCAAC
    CTGTTGACCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG TGTGGCCAAG
    ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG
    GCCTCACAGG TACACCACAG CCGATCCAAC CCCACGGACA TCTATCCCTC CAAGTGGATC
    GCAAGACTCC GCCACATTAA GCGTCTCCGT CAGCGGATCC GTGAAGAAGT GCACTATTCA
    AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCATACCA AAGTCCCAGC TCAAGCCCCT
    ACGGAGGCTA CACCCACCTA CGAGACAGGC CAGCGGAAAC GCCTCATTTC CTCTGTGGAT
    GACTTCACAG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57184
    Clone ID Related Accession (Same CDS sequence) XM_017597022.1
    Accession Version XM_017597022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTCGTG
    AACCTGGTCA AGTTCAACAG CTGCTACCTT GACGAATACA TTGCGCCAAT GGTTCACATG
    ATTTGTCTGT TATGTATCCG GACAGTGTCC TCTGTGGACA TCGAGGTGTC CTTGCAAGTG
    CTGGATGCTG TGGTCTGCTA CAACTGCCTG CCTGCTGAGA GCCTGCCTCT GTTCATTATC
    ACCCTGTGCC GCACCGTCAA CGTCAAGGAG CTGTGTGAGC CGTGCTGGAA GCTGATGCGA
    AACCTTCTAG GTACCCATCT AGGCCACAGT GCCATTTACA ACATGTGCCG TATCATGGAG
    AACAGATCCT ACATGGAAGA CGCCCCCTTG TTGAGGGGAG CCGTGTTCTT TGTTGGGATG
    GCGCTCTGGG GAGCTCACCG ACTCTATTCG CTCAAGAACT CCCCCACATC TGTGCTGCCG
    TCTTTTTATG AGGCCATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC
    ATAACAAGAC TCATCAAGAA GTACAGGAAG GAGCTCCAGG CTGTGACATG GGACATTCTG
    CTGGACATCA TTGAACGACT GCTCCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAGG
    ACCATTGTCC ATGACCTGCT GACCACTGTG GAGGAGCTGT GTGACCAGAA CGAGTTCCAC
    GGATCGCAAG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG GCCTGAATCC
    TCTCTCTTAA ACCTGATAAC CTACAGAGCC CAGTCTATCC ACCCAGCCAA GGATGGCTGG
    ATCCAGAACC TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ACGAGTGCCG CAGTGCCGTG
    CGCATCAAGG TGCTGGACGT GCTGTCCTTC GTGCTCCTCA TCAACAGGCA GTTCTACGAG
    GAGGAGCTGA TTAACTCGGT GGTCATCTCA CAGCTCTCCC ACATCCCCGA GGATAAAGAC
    CATCAGGTTC GAAAGCTGGC TACCCAGCTG CTGGTGGACC TGGCAGAGGG CTGCCACACC
    CACCACTTCA ACAGTCTGTT GGACATCATT GAAAAGGTGA TGGCTCGCTC CCTTTCTCCA
    CCCCTGGAGC TGGAGGAAAG GGATCTGGCT GTGTACTCGG CCTCCCTGGA GGATGTGAAG
    ACGGCAGTCC TCGGGCTCCT GGTCATCCTT CAGACCAAGC TGTATACCTT GCCCGCCAGC
    CATGCCACAC GAGTGTATGA GACACTCATT AGCCACATCC AGCTCCATTA CAAGCACGGC
    TACTCCTTGC CCATTGCTAG CAGCATCCGG CTGCAGGCCT TTGACTTCCT GCTGCTACTG
    CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GCGATTCAGC
    CCTTACTGCC TCTGTGACTG CGCGGAACTG GACAGGGCCT CAGAGAAGAA AGCCAGCGGA
    CCCCTTTCAC CGCCAACTGG GCCCCCCAGC CCTGTGCCAA CGGGCCCTGC TGTGAGGCTC
    GGCCACCTGC CCTACTCCCT GCTTTTCCGT GTCCTGCTGC AGTGTTTGAA GCAGGAGACC
    GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AAACTCCCTG AGTCGCTGCG CTACAAAGTC
    CTCATTTTCA CCTCCCCCTG CAGTGTTGAC CAGCTGTCTT CTGCTCTCTG CTCCATGCTT
    TCAGCCCCGA AGACCCTTGA GCGGCTCCGA GGCACCCCAG AAGGCTTCTC CAGAACTGAC
    CTGCACTTGG CTGTGGTCCC TGTACTGACA GCGTTAATAT CTTACCACAA CTATTTGGAT
    AAGACTAGAC AGCGTGAGAT GGTTTACTGC CTAGAACAAG GCCTTATCTA CCGCTGTGCC
    AGCCAGTGTG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG
    GCGCTCCCTG TCCTGGTGGT GAAGCTGACA CACATCTCTG CTACAGCTAG CATGGCCATC
    CCCCTCCTGG AGTTCCTGTC TACCTTGGCC AGGCTGCCTC ACCTCTACAG GAACTTCGCC
    GCAGAGCAGT ATGCAAGTGT GTTTGCCATC TCCTTGCCCT ACACCAACCC CTCCAAGTTC
    AACCAGTACA TTGTGTGTCT GGCCCATCAC GTCATAGCCA TGTGGTTCAT TAGGTGCCGA
    CTGCCCTTTC GGAAGGATTT TGTCCCTTAT ATCACTAAGG GTCTGCGTTC CAATGTCCTC
    CTGTCCTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT
    GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA
    CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT
    GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCTC TTACCAGTGC CAGCTTGGGG
    TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACC TGAAGAATCT CCACTTGGAA
    CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAACTT CACTGCGGTT
    CCCAAGAGGT CCCCTGTGGG AGAATTCCTC CTGGCAGGGG GCAGGACCAA AACGTGGCTG
    GTTGGAAATA AGCTGGTCAC TGTGACAACG AGTGTGGGAA CTGGTACGCG GTCGTTGCTG
    GGCCTGGACT CCGGGGATCT GCAGGGTGGC TCAGCATCAA GCTCTGATCC TGGCACACAT
    GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC
    CGTGGGGCCC GGGACCGAGT CCGCTCCATG TCAGGGGGCC ACGGCCTTCG AGTTGGTGTC
    CTGGACACTT CAGCTCCCTA CACCCCAGGA GGCCCTGCTT CTCTGGGAGC ACAGGCAGCA
    CCAGCAGCAA GGCCTGAGAA GCCCTGTGCA GGAGCCCAAC TTCCGGCAGC GGAGAAGGCG
    AACCTGGCAG CCTATGTGCC TTTGCTGACC CAGGGCTGGG CAGAGATCCT GGTCCGCAGG
    CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTTTCCTCA
    GACATCAACA ACATGCCCCT GCAAGAGCTG TCTAATGCCC TTATGGCTGC TGAGCGCTTC
    AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACAGCC
    AAGCCTCCTA CTCTCCCACG TTCTAACACA GACTCGGCCG TGGTTCTTGA GGAGGGAAGT
    CCAGGAGAGG CTCATGTGCC AGTGGAGCCC CCCGAATTGG AGGACTTTGA GGCAGCATTA
    GGTACAGACA GGCACTGCCA GCGGCCTGAC GCCTACAGCA GGTCTTCCTC AGCATCTAGC
    CAGGAAGAAA AGTCCCACTT GGAGGAGCTG GCTGCAGGGG GTATCCCCAT TGAGCGGGCC
    ATCTCCTCTG AGGGGGCTCG GCCTACAGTG GACCTGTCCT TCCAACCCTC ACAGCCTTTG
    AGCAAGTCTA GCTCTTCTCC TGAGTTACAG ACCTTACAGG ACATCCTTGG AGACCTAGGG
    GACAAGACTG ACATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGATCCCA GTCGGGGATC
    CTGGATGGGG AAGCAGCTAC CTGGTCAGCT CCGGGTGAAG AGAGCCGTAT CACAGTCCCA
    CCTGAAGGTC CCCTGCCTTC CAGTTCTCCC CGCTCCCCTA GTGGCCTCCG GCCCCGAGGC
    TATACCATCT CTGATTCAGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGACAACTTC
    AAGAGCAGAA CTGCGGCCTC CAGTGCTGAA AAGGTGCCAG GCATCAACCC TAGCTTTGTG
    TTCCTACAGC TCTACCATTC ACCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTGTTG
    CCCAACGAGT CCTTTGAACG GTCAGTGCAG CTCCTTGACC AGATCCCATC CTATGACACT
    CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTA
    TCAAATGAGC ATGGCTCTTA TAGGTACACA GAGTTTCTGA CAGGCCTGGG TCGGCTTATT
    GAGCTGAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT GTGTGGCGAG
    GATGGACAGT TCACGTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCT
    ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGTTGTG ACAAGAAACG GCACCTGGGC
    AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAGCT GGGCACCATT
    AAGGGCCAGT TCAACTTCGT CCATGTGATC ATCACACCGC TGGACTATAA ATGCAACCTG
    TTGACCCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ACACCAGTGT GGCCAAGATT
    GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC
    TCACAGGTAC ACCACAGCCG ATCCAACCCC ACGGACATCT ATCCCTCCAA GTGGATCGCA
    AGACTCCGCC ACATTAAGCG TCTCCGTCAG CGGATCCGTG AAGAAGTGCA CTATTCAAAC
    CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CATACCAAAG TCCCAGCTCA AGCCCCTACG
    GAGGCTACAC CCACCTACGA GACAGGCCAG CGGAAACGCC TCATTTCCTC TGTGGATGAC
    TTCACAGAGT TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452511.1
    CDS203..5560
    Translation

    Target ORF information:

    RefSeq Version XM_017597022.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTCGTG
    AACCTGGTCA AGTTCAACAG CTGCTACCTT GACGAATACA TTGCGCCAAT GGTTCACATG
    ATTTGTCTGT TATGTATCCG GACAGTGTCC TCTGTGGACA TCGAGGTGTC CTTGCAAGTG
    CTGGATGCTG TGGTCTGCTA CAACTGCCTG CCTGCTGAGA GCCTGCCTCT GTTCATTATC
    ACCCTGTGCC GCACCGTCAA CGTCAAGGAG CTGTGTGAGC CGTGCTGGAA GCTGATGCGA
    AACCTTCTAG GTACCCATCT AGGCCACAGT GCCATTTACA ACATGTGCCG TATCATGGAG
    AACAGATCCT ACATGGAAGA CGCCCCCTTG TTGAGGGGAG CCGTGTTCTT TGTTGGGATG
    GCGCTCTGGG GAGCTCACCG ACTCTATTCG CTCAAGAACT CCCCCACATC TGTGCTGCCG
    TCTTTTTATG AGGCCATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC
    ATAACAAGAC TCATCAAGAA GTACAGGAAG GAGCTCCAGG CTGTGACATG GGACATTCTG
    CTGGACATCA TTGAACGACT GCTCCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAGG
    ACCATTGTCC ATGACCTGCT GACCACTGTG GAGGAGCTGT GTGACCAGAA CGAGTTCCAC
    GGATCGCAAG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG GCCTGAATCC
    TCTCTCTTAA ACCTGATAAC CTACAGAGCC CAGTCTATCC ACCCAGCCAA GGATGGCTGG
    ATCCAGAACC TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ACGAGTGCCG CAGTGCCGTG
    CGCATCAAGG TGCTGGACGT GCTGTCCTTC GTGCTCCTCA TCAACAGGCA GTTCTACGAG
    GAGGAGCTGA TTAACTCGGT GGTCATCTCA CAGCTCTCCC ACATCCCCGA GGATAAAGAC
    CATCAGGTTC GAAAGCTGGC TACCCAGCTG CTGGTGGACC TGGCAGAGGG CTGCCACACC
    CACCACTTCA ACAGTCTGTT GGACATCATT GAAAAGGTGA TGGCTCGCTC CCTTTCTCCA
    CCCCTGGAGC TGGAGGAAAG GGATCTGGCT GTGTACTCGG CCTCCCTGGA GGATGTGAAG
    ACGGCAGTCC TCGGGCTCCT GGTCATCCTT CAGACCAAGC TGTATACCTT GCCCGCCAGC
    CATGCCACAC GAGTGTATGA GACACTCATT AGCCACATCC AGCTCCATTA CAAGCACGGC
    TACTCCTTGC CCATTGCTAG CAGCATCCGG CTGCAGGCCT TTGACTTCCT GCTGCTACTG
    CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GCGATTCAGC
    CCTTACTGCC TCTGTGACTG CGCGGAACTG GACAGGGCCT CAGAGAAGAA AGCCAGCGGA
    CCCCTTTCAC CGCCAACTGG GCCCCCCAGC CCTGTGCCAA CGGGCCCTGC TGTGAGGCTC
    GGCCACCTGC CCTACTCCCT GCTTTTCCGT GTCCTGCTGC AGTGTTTGAA GCAGGAGACC
    GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AAACTCCCTG AGTCGCTGCG CTACAAAGTC
    CTCATTTTCA CCTCCCCCTG CAGTGTTGAC CAGCTGTCTT CTGCTCTCTG CTCCATGCTT
    TCAGCCCCGA AGACCCTTGA GCGGCTCCGA GGCACCCCAG AAGGCTTCTC CAGAACTGAC
    CTGCACTTGG CTGTGGTCCC TGTACTGACA GCGTTAATAT CTTACCACAA CTATTTGGAT
    AAGACTAGAC AGCGTGAGAT GGTTTACTGC CTAGAACAAG GCCTTATCTA CCGCTGTGCC
    AGCCAGTGTG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG
    GCGCTCCCTG TCCTGGTGGT GAAGCTGACA CACATCTCTG CTACAGCTAG CATGGCCATC
    CCCCTCCTGG AGTTCCTGTC TACCTTGGCC AGGCTGCCTC ACCTCTACAG GAACTTCGCC
    GCAGAGCAGT ATGCAAGTGT GTTTGCCATC TCCTTGCCCT ACACCAACCC CTCCAAGTTC
    AACCAGTACA TTGTGTGTCT GGCCCATCAC GTCATAGCCA TGTGGTTCAT TAGGTGCCGA
    CTGCCCTTTC GGAAGGATTT TGTCCCTTAT ATCACTAAGG GTCTGCGTTC CAATGTCCTC
    CTGTCCTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT
    GAGAGACCCA AAAGTTTGAG GATAGCCAGA GCCCCCAAAC AAGGCCTGAA TAACTCTCCA
    CCTGTGAAAG AATTCAAAGA GAGCTGTGCA GCCGAGGCCT TCCGGTGCCG CAGCATCAGT
    GTATCTGAAC ATGTGGTCCG CAGCAGAATA CAGACATCTC TTACCAGTGC CAGCTTGGGG
    TCTGCGGATG AGAACTCTAT GGCCCAGGCT GATGACAACC TGAAGAATCT CCACTTGGAA
    CTCACAGAAA CATGTCTGGA CATGATGGCC CGATATGTGT TCTCCAACTT CACTGCGGTT
    CCCAAGAGGT CCCCTGTGGG AGAATTCCTC CTGGCAGGGG GCAGGACCAA AACGTGGCTG
    GTTGGAAATA AGCTGGTCAC TGTGACAACG AGTGTGGGAA CTGGTACGCG GTCGTTGCTG
    GGCCTGGACT CCGGGGATCT GCAGGGTGGC TCAGCATCAA GCTCTGATCC TGGCACACAT
    GTGAGGCAGA CAAAGGAAGC ACCGGCCAAA CTGGAGTCCC AGGCTGGGCA GCAGGTGTCC
    CGTGGGGCCC GGGACCGAGT CCGCTCCATG TCAGGGGGCC ACGGCCTTCG AGTTGGTGTC
    CTGGACACTT CAGCTCCCTA CACCCCAGGA GGCCCTGCTT CTCTGGGAGC ACAGGCAGCA
    CCAGCAGCAA GGCCTGAGAA GCCCTGTGCA GGAGCCCAAC TTCCGGCAGC GGAGAAGGCG
    AACCTGGCAG CCTATGTGCC TTTGCTGACC CAGGGCTGGG CAGAGATCCT GGTCCGCAGG
    CCCACAGGAA ACACCAGCTG GCTGATGAGC TTGGAGAACC CGCTCAGCCC CTTTTCCTCA
    GACATCAACA ACATGCCCCT GCAAGAGCTG TCTAATGCCC TTATGGCTGC TGAGCGCTTC
    AAGGAGCACC GGGACACGGC CCTGTACAAG TCATTGTCAG TGCCAGCGGC TGGCACAGCC
    AAGCCTCCTA CTCTCCCACG TTCTAACACA GACTCGGCCG TGGTTCTTGA GGAGGGAAGT
    CCAGGAGAGG CTCATGTGCC AGTGGAGCCC CCCGAATTGG AGGACTTTGA GGCAGCATTA
    GGTACAGACA GGCACTGCCA GCGGCCTGAC GCCTACAGCA GGTCTTCCTC AGCATCTAGC
    CAGGAAGAAA AGTCCCACTT GGAGGAGCTG GCTGCAGGGG GTATCCCCAT TGAGCGGGCC
    ATCTCCTCTG AGGGGGCTCG GCCTACAGTG GACCTGTCCT TCCAACCCTC ACAGCCTTTG
    AGCAAGTCTA GCTCTTCTCC TGAGTTACAG ACCTTACAGG ACATCCTTGG AGACCTAGGG
    GACAAGACTG ACATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGATCCCA GTCGGGGATC
    CTGGATGGGG AAGCAGCTAC CTGGTCAGCT CCGGGTGAAG AGAGCCGTAT CACAGTCCCA
    CCTGAAGGTC CCCTGCCTTC CAGTTCTCCC CGCTCCCCTA GTGGCCTCCG GCCCCGAGGC
    TATACCATCT CTGATTCAGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGACAACTTC
    AAGAGCAGAA CTGCGGCCTC CAGTGCTGAA AAGGTGCCAG GCATCAACCC TAGCTTTGTG
    TTCCTACAGC TCTACCATTC ACCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTGTTG
    CCCAACGAGT CCTTTGAACG GTCAGTGCAG CTCCTTGACC AGATCCCATC CTATGACACT
    CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTA
    TCAAATGAGC ATGGCTCTTA TAGGTACACA GAGTTTCTGA CAGGCCTGGG TCGGCTTATT
    GAGCTGAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT GTGTGGCGAG
    GATGGACAGT TCACGTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCT
    ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGTTGTG ACAAGAAACG GCACCTGGGC
    AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAGCT GGGCACCATT
    AAGGGCCAGT TCAACTTCGT CCATGTGATC ATCACACCGC TGGACTATAA ATGCAACCTG
    TTGACCCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ACACCAGTGT GGCCAAGATT
    GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC
    TCACAGGTAC ACCACAGCCG ATCCAACCCC ACGGACATCT ATCCCTCCAA GTGGATCGCA
    AGACTCCGCC ACATTAAGCG TCTCCGTCAG CGGATCCGTG AAGAAGTGCA CTATTCAAAC
    CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CATACCAAAG TCCCAGCTCA AGCCCCTACG
    GAGGCTACAC CCACCTACGA GACAGGCCAG CGGAAACGCC TCATTTCCTC TGTGGATGAC
    TTCACAGAGT TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57185
    Clone ID Related Accession (Same CDS sequence) XM_017597023.1
    Accession Version XM_017597023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGCAG AATACAGACA TCTCTTACCA GTGCCAGCTT GGGGTCTGCG
    GATGAGAACT CTATGGCCCA GGCTGATGAC AACCTGAAGA ATCTCCACTT GGAACTCACA
    GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ACTTCACTGC GGTTCCCAAG
    AGGTCCCCTG TGGGAGAATT CCTCCTGGCA GGGGGCAGGA CCAAAACGTG GCTGGTTGGA
    AATAAGCTGG TCACTGTGAC AACGAGTGTG GGAACTGGTA CGCGGTCGTT GCTGGGCCTG
    GACTCCGGGG ATCTGCAGGG TGGCTCAGCA TCAAGCTCTG ATCCTGGCAC ACATGTGAGG
    CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG
    GCCCGGGACC GAGTCCGCTC CATGTCAGGG GGCCACGGCC TTCGAGTTGG TGTCCTGGAC
    ACTTCAGCTC CCTACACCCC AGGAGGCCCT GCTTCTCTGG GAGCACAGGC AGCACCAGCA
    GCAAGGCCTG AGAAGCCCTG TGCAGGAGCC CAACTTCCGG CAGCGGAGAA GGCGAACCTG
    GCAGCCTATG TGCCTTTGCT GACCCAGGGC TGGGCAGAGA TCCTGGTCCG CAGGCCCACA
    GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTTTC CTCAGACATC
    AACAACATGC CCCTGCAAGA GCTGTCTAAT GCCCTTATGG CTGCTGAGCG CTTCAAGGAG
    CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCT
    CCTACTCTCC CACGTTCTAA CACAGTGGCT TCTTTCTCCT CCCTGTACCA GCCCAGCTGC
    CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCGG CCGTGGTTCT TGAGGAGGGA
    AGTCCAGGAG AGGCTCATGT GCCAGTGGAG CCCCCCGAAT TGGAGGACTT TGAGGCAGCA
    TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACGCCTACA GCAGGTCTTC CTCAGCATCT
    AGCCAGGAAG AAAAGTCCCA CTTGGAGGAG CTGGCTGCAG GGGGTATCCC CATTGAGCGG
    GCCATCTCCT CTGAGGGGGC TCGGCCTACA GTGGACCTGT CCTTCCAACC CTCACAGCCT
    TTGAGCAAGT CTAGCTCTTC TCCTGAGTTA CAGACCTTAC AGGACATCCT TGGAGACCTA
    GGGGACAAGA CTGACATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGATC CCAGTCGGGG
    ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTCCGGGTG AAGAGAGCCG TATCACAGTC
    CCACCTGAAG GTCCCCTGCC TTCCAGTTCT CCCCGCTCCC CTAGTGGCCT CCGGCCCCGA
    GGCTATACCA TCTCTGATTC AGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGACAAC
    TTCAAGAGCA GAACTGCGGC CTCCAGTGCT GAAAAGGTGC CAGGCATCAA CCCTAGCTTT
    GTGTTCCTAC AGCTCTACCA TTCACCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTG
    TTGCCCAACG AGTCCTTTGA ACGGTCAGTG CAGCTCCTTG ACCAGATCCC ATCCTATGAC
    ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC
    CTATCAAATG AGCATGGCTC TTATAGGTAC ACAGAGTTTC TGACAGGCCT GGGTCGGCTT
    ATTGAGCTGA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTGTGTGGC
    GAGGATGGAC AGTTCACGTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT
    GCTACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGTT GTGACAAGAA ACGGCACCTG
    GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA GCTGGGCACC
    ATTAAGGGCC AGTTCAACTT CGTCCATGTG ATCATCACAC CGCTGGACTA TAAATGCAAC
    CTGTTGACCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG TGTGGCCAAG
    ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG
    GCCTCACAGG TACACCACAG CCGATCCAAC CCCACGGACA TCTATCCCTC CAAGTGGATC
    GCAAGACTCC GCCACATTAA GCGTCTCCGT CAGCGGATCC GTGAAGAAGT GCACTATTCA
    AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCATACCA AAGTCCCAGC TCAAGCCCCT
    ACGGAGGCTA CACCCACCTA CGAGACAGGC CAGCGGAAAC GCCTCATTTC CTCTGTGGAT
    GACTTCACAG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452512.1
    CDS202..5502
    Translation

    Target ORF information:

    RefSeq Version XM_017597023.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGCAG AATACAGACA TCTCTTACCA GTGCCAGCTT GGGGTCTGCG
    GATGAGAACT CTATGGCCCA GGCTGATGAC AACCTGAAGA ATCTCCACTT GGAACTCACA
    GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ACTTCACTGC GGTTCCCAAG
    AGGTCCCCTG TGGGAGAATT CCTCCTGGCA GGGGGCAGGA CCAAAACGTG GCTGGTTGGA
    AATAAGCTGG TCACTGTGAC AACGAGTGTG GGAACTGGTA CGCGGTCGTT GCTGGGCCTG
    GACTCCGGGG ATCTGCAGGG TGGCTCAGCA TCAAGCTCTG ATCCTGGCAC ACATGTGAGG
    CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG
    GCCCGGGACC GAGTCCGCTC CATGTCAGGG GGCCACGGCC TTCGAGTTGG TGTCCTGGAC
    ACTTCAGCTC CCTACACCCC AGGAGGCCCT GCTTCTCTGG GAGCACAGGC AGCACCAGCA
    GCAAGGCCTG AGAAGCCCTG TGCAGGAGCC CAACTTCCGG CAGCGGAGAA GGCGAACCTG
    GCAGCCTATG TGCCTTTGCT GACCCAGGGC TGGGCAGAGA TCCTGGTCCG CAGGCCCACA
    GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTTTC CTCAGACATC
    AACAACATGC CCCTGCAAGA GCTGTCTAAT GCCCTTATGG CTGCTGAGCG CTTCAAGGAG
    CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCT
    CCTACTCTCC CACGTTCTAA CACAGTGGCT TCTTTCTCCT CCCTGTACCA GCCCAGCTGC
    CAAGGACAGC TGCACAGGAG CGTTTCCTGG GCAGACTCGG CCGTGGTTCT TGAGGAGGGA
    AGTCCAGGAG AGGCTCATGT GCCAGTGGAG CCCCCCGAAT TGGAGGACTT TGAGGCAGCA
    TTAGGTACAG ACAGGCACTG CCAGCGGCCT GACGCCTACA GCAGGTCTTC CTCAGCATCT
    AGCCAGGAAG AAAAGTCCCA CTTGGAGGAG CTGGCTGCAG GGGGTATCCC CATTGAGCGG
    GCCATCTCCT CTGAGGGGGC TCGGCCTACA GTGGACCTGT CCTTCCAACC CTCACAGCCT
    TTGAGCAAGT CTAGCTCTTC TCCTGAGTTA CAGACCTTAC AGGACATCCT TGGAGACCTA
    GGGGACAAGA CTGACATTGG ACGGCTGAGT CCTGAGGCTA AGGTCCGATC CCAGTCGGGG
    ATCCTGGATG GGGAAGCAGC TACCTGGTCA GCTCCGGGTG AAGAGAGCCG TATCACAGTC
    CCACCTGAAG GTCCCCTGCC TTCCAGTTCT CCCCGCTCCC CTAGTGGCCT CCGGCCCCGA
    GGCTATACCA TCTCTGATTC AGCTCCATCA CGAAGGGGAA AGAGGGTAGA AAGGGACAAC
    TTCAAGAGCA GAACTGCGGC CTCCAGTGCT GAAAAGGTGC CAGGCATCAA CCCTAGCTTT
    GTGTTCCTAC AGCTCTACCA TTCACCCTTT TTTGGTGATG AGTCCAATAA GCCCATCCTG
    TTGCCCAACG AGTCCTTTGA ACGGTCAGTG CAGCTCCTTG ACCAGATCCC ATCCTATGAC
    ACTCACAAGA TTGCTGTCCT GTATGTGGGA GAAGGCCAGA GCAGCAGTGA GCTGGCCATC
    CTATCAAATG AGCATGGCTC TTATAGGTAC ACAGAGTTTC TGACAGGCCT GGGTCGGCTT
    ATTGAGCTGA AGGACTGCCA ACCAGACAAG GTGTACTTAG GTGGACTGGA TGTGTGTGGC
    GAGGATGGAC AGTTCACGTA CTGCTGGCAT GATGACATCA TGCAAGCTGT TTTCCACATT
    GCTACCCTGA TGCCCACCAA GGATGTGGAC AAGCACCGTT GTGACAAGAA ACGGCACCTG
    GGCAATGACT TTGTTTCTAT CATCTACAAT GATTCTGGTG AGGACTTCAA GCTGGGCACC
    ATTAAGGGCC AGTTCAACTT CGTCCATGTG ATCATCACAC CGCTGGACTA TAAATGCAAC
    CTGTTGACCC TGCAGTGCAG GAAAGACATG GAGGGCCTTG TGGACACCAG TGTGGCCAAG
    ATTGTGTCTG ACCGCAACCT GTCCTTTGTG GCCCGACAGA TGGCCCTGCA TGCAAATATG
    GCCTCACAGG TACACCACAG CCGATCCAAC CCCACGGACA TCTATCCCTC CAAGTGGATC
    GCAAGACTCC GCCACATTAA GCGTCTCCGT CAGCGGATCC GTGAAGAAGT GCACTATTCA
    AACCCAAGCT TGCCTCTGAT GCACCCTCCA GCCCATACCA AAGTCCCAGC TCAAGCCCCT
    ACGGAGGCTA CACCCACCTA CGAGACAGGC CAGCGGAAAC GCCTCATTTC CTCTGTGGAT
    GACTTCACAG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57186
    Clone ID Related Accession (Same CDS sequence) XM_017597024.1
    Accession Version XM_017597024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGAAT ACAGACATCT CTTACCAGTG CCAGCTTGGG GTCTGCGGAT
    GAGAACTCTA TGGCCCAGGC TGATGACAAC CTGAAGAATC TCCACTTGGA ACTCACAGAA
    ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAACT TCACTGCGGT TCCCAAGAGG
    TCCCCTGTGG GAGAATTCCT CCTGGCAGGG GGCAGGACCA AAACGTGGCT GGTTGGAAAT
    AAGCTGGTCA CTGTGACAAC GAGTGTGGGA ACTGGTACGC GGTCGTTGCT GGGCCTGGAC
    TCCGGGGATC TGCAGGGTGG CTCAGCATCA AGCTCTGATC CTGGCACACA TGTGAGGCAG
    ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGAG TCCGCTCCAT GTCAGGGGGC CACGGCCTTC GAGTTGGTGT CCTGGACACT
    TCAGCTCCCT ACACCCCAGG AGGCCCTGCT TCTCTGGGAG CACAGGCAGC ACCAGCAGCA
    AGGCCTGAGA AGCCCTGTGC AGGAGCCCAA CTTCCGGCAG CGGAGAAGGC GAACCTGGCA
    GCCTATGTGC CTTTGCTGAC CCAGGGCTGG GCAGAGATCC TGGTCCGCAG GCCCACAGGA
    AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTTTCCTC AGACATCAAC
    AACATGCCCC TGCAAGAGCT GTCTAATGCC CTTATGGCTG CTGAGCGCTT CAAGGAGCAC
    CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCTCCT
    ACTCTCCCAC GTTCTAACAC AGTGGCTTCT TTCTCCTCCC TGTACCAGCC CAGCTGCCAA
    GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCGGCCG TGGTTCTTGA GGAGGGAAGT
    CCAGGAGAGG CTCATGTGCC AGTGGAGCCC CCCGAATTGG AGGACTTTGA GGCAGCATTA
    GGTACAGACA GGCACTGCCA GCGGCCTGAC GCCTACAGCA GGTCTTCCTC AGCATCTAGC
    CAGGAAGAAA AGTCCCACTT GGAGGAGCTG GCTGCAGGGG GTATCCCCAT TGAGCGGGCC
    ATCTCCTCTG AGGGGGCTCG GCCTACAGTG GACCTGTCCT TCCAACCCTC ACAGCCTTTG
    AGCAAGTCTA GCTCTTCTCC TGAGTTACAG ACCTTACAGG ACATCCTTGG AGACCTAGGG
    GACAAGACTG ACATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGATCCCA GTCGGGGATC
    CTGGATGGGG AAGCAGCTAC CTGGTCAGCT CCGGGTGAAG AGAGCCGTAT CACAGTCCCA
    CCTGAAGGTC CCCTGCCTTC CAGTTCTCCC CGCTCCCCTA GTGGCCTCCG GCCCCGAGGC
    TATACCATCT CTGATTCAGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGACAACTTC
    AAGAGCAGAA CTGCGGCCTC CAGTGCTGAA AAGGTGCCAG GCATCAACCC TAGCTTTGTG
    TTCCTACAGC TCTACCATTC ACCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTGTTG
    CCCAACGAGT CCTTTGAACG GTCAGTGCAG CTCCTTGACC AGATCCCATC CTATGACACT
    CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTA
    TCAAATGAGC ATGGCTCTTA TAGGTACACA GAGTTTCTGA CAGGCCTGGG TCGGCTTATT
    GAGCTGAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT GTGTGGCGAG
    GATGGACAGT TCACGTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCT
    ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGTTGTG ACAAGAAACG GCACCTGGGC
    AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAGCT GGGCACCATT
    AAGGGCCAGT TCAACTTCGT CCATGTGATC ATCACACCGC TGGACTATAA ATGCAACCTG
    TTGACCCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ACACCAGTGT GGCCAAGATT
    GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC
    TCACAGGTAC ACCACAGCCG ATCCAACCCC ACGGACATCT ATCCCTCCAA GTGGATCGCA
    AGACTCCGCC ACATTAAGCG TCTCCGTCAG CGGATCCGTG AAGAAGTGCA CTATTCAAAC
    CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CATACCAAAG TCCCAGCTCA AGCCCCTACG
    GAGGCTACAC CCACCTACGA GACAGGCCAG CGGAAACGCC TCATTTCCTC TGTGGATGAC
    TTCACAGAGT TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452513.1
    CDS202..5499
    Translation

    Target ORF information:

    RefSeq Version XM_017597024.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGAAT ACAGACATCT CTTACCAGTG CCAGCTTGGG GTCTGCGGAT
    GAGAACTCTA TGGCCCAGGC TGATGACAAC CTGAAGAATC TCCACTTGGA ACTCACAGAA
    ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAACT TCACTGCGGT TCCCAAGAGG
    TCCCCTGTGG GAGAATTCCT CCTGGCAGGG GGCAGGACCA AAACGTGGCT GGTTGGAAAT
    AAGCTGGTCA CTGTGACAAC GAGTGTGGGA ACTGGTACGC GGTCGTTGCT GGGCCTGGAC
    TCCGGGGATC TGCAGGGTGG CTCAGCATCA AGCTCTGATC CTGGCACACA TGTGAGGCAG
    ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGAG TCCGCTCCAT GTCAGGGGGC CACGGCCTTC GAGTTGGTGT CCTGGACACT
    TCAGCTCCCT ACACCCCAGG AGGCCCTGCT TCTCTGGGAG CACAGGCAGC ACCAGCAGCA
    AGGCCTGAGA AGCCCTGTGC AGGAGCCCAA CTTCCGGCAG CGGAGAAGGC GAACCTGGCA
    GCCTATGTGC CTTTGCTGAC CCAGGGCTGG GCAGAGATCC TGGTCCGCAG GCCCACAGGA
    AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTTTCCTC AGACATCAAC
    AACATGCCCC TGCAAGAGCT GTCTAATGCC CTTATGGCTG CTGAGCGCTT CAAGGAGCAC
    CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCTCCT
    ACTCTCCCAC GTTCTAACAC AGTGGCTTCT TTCTCCTCCC TGTACCAGCC CAGCTGCCAA
    GGACAGCTGC ACAGGAGCGT TTCCTGGGCA GACTCGGCCG TGGTTCTTGA GGAGGGAAGT
    CCAGGAGAGG CTCATGTGCC AGTGGAGCCC CCCGAATTGG AGGACTTTGA GGCAGCATTA
    GGTACAGACA GGCACTGCCA GCGGCCTGAC GCCTACAGCA GGTCTTCCTC AGCATCTAGC
    CAGGAAGAAA AGTCCCACTT GGAGGAGCTG GCTGCAGGGG GTATCCCCAT TGAGCGGGCC
    ATCTCCTCTG AGGGGGCTCG GCCTACAGTG GACCTGTCCT TCCAACCCTC ACAGCCTTTG
    AGCAAGTCTA GCTCTTCTCC TGAGTTACAG ACCTTACAGG ACATCCTTGG AGACCTAGGG
    GACAAGACTG ACATTGGACG GCTGAGTCCT GAGGCTAAGG TCCGATCCCA GTCGGGGATC
    CTGGATGGGG AAGCAGCTAC CTGGTCAGCT CCGGGTGAAG AGAGCCGTAT CACAGTCCCA
    CCTGAAGGTC CCCTGCCTTC CAGTTCTCCC CGCTCCCCTA GTGGCCTCCG GCCCCGAGGC
    TATACCATCT CTGATTCAGC TCCATCACGA AGGGGAAAGA GGGTAGAAAG GGACAACTTC
    AAGAGCAGAA CTGCGGCCTC CAGTGCTGAA AAGGTGCCAG GCATCAACCC TAGCTTTGTG
    TTCCTACAGC TCTACCATTC ACCCTTTTTT GGTGATGAGT CCAATAAGCC CATCCTGTTG
    CCCAACGAGT CCTTTGAACG GTCAGTGCAG CTCCTTGACC AGATCCCATC CTATGACACT
    CACAAGATTG CTGTCCTGTA TGTGGGAGAA GGCCAGAGCA GCAGTGAGCT GGCCATCCTA
    TCAAATGAGC ATGGCTCTTA TAGGTACACA GAGTTTCTGA CAGGCCTGGG TCGGCTTATT
    GAGCTGAAGG ACTGCCAACC AGACAAGGTG TACTTAGGTG GACTGGATGT GTGTGGCGAG
    GATGGACAGT TCACGTACTG CTGGCATGAT GACATCATGC AAGCTGTTTT CCACATTGCT
    ACCCTGATGC CCACCAAGGA TGTGGACAAG CACCGTTGTG ACAAGAAACG GCACCTGGGC
    AATGACTTTG TTTCTATCAT CTACAATGAT TCTGGTGAGG ACTTCAAGCT GGGCACCATT
    AAGGGCCAGT TCAACTTCGT CCATGTGATC ATCACACCGC TGGACTATAA ATGCAACCTG
    TTGACCCTGC AGTGCAGGAA AGACATGGAG GGCCTTGTGG ACACCAGTGT GGCCAAGATT
    GTGTCTGACC GCAACCTGTC CTTTGTGGCC CGACAGATGG CCCTGCATGC AAATATGGCC
    TCACAGGTAC ACCACAGCCG ATCCAACCCC ACGGACATCT ATCCCTCCAA GTGGATCGCA
    AGACTCCGCC ACATTAAGCG TCTCCGTCAG CGGATCCGTG AAGAAGTGCA CTATTCAAAC
    CCAAGCTTGC CTCTGATGCA CCCTCCAGCC CATACCAAAG TCCCAGCTCA AGCCCCTACG
    GAGGCTACAC CCACCTACGA GACAGGCCAG CGGAAACGCC TCATTTCCTC TGTGGATGAC
    TTCACAGAGT TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57187
    Clone ID Related Accession (Same CDS sequence) XM_017597025.1
    Accession Version XM_017597025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGCAG AATACAGACA TCTCTTACCA GTGCCAGCTT GGGGTCTGCG
    GATGAGAACT CTATGGCCCA GGCTGATGAC AACCTGAAGA ATCTCCACTT GGAACTCACA
    GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ACTTCACTGC GGTTCCCAAG
    AGGTCCCCTG TGGGAGAATT CCTCCTGGCA GGGGGCAGGA CCAAAACGTG GCTGGTTGGA
    AATAAGCTGG TCACTGTGAC AACGAGTGTG GGAACTGGTA CGCGGTCGTT GCTGGGCCTG
    GACTCCGGGG ATCTGCAGGG TGGCTCAGCA TCAAGCTCTG ATCCTGGCAC ACATGTGAGG
    CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG
    GCCCGGGACC GAGTCCGCTC CATGTCAGGG GGCCACGGCC TTCGAGTTGG TGTCCTGGAC
    ACTTCAGCTC CCTACACCCC AGGAGGCCCT GCTTCTCTGG GAGCACAGGC AGCACCAGCA
    GCAAGGCCTG AGAAGCCCTG TGCAGGAGCC CAACTTCCGG CAGCGGAGAA GGCGAACCTG
    GCAGCCTATG TGCCTTTGCT GACCCAGGGC TGGGCAGAGA TCCTGGTCCG CAGGCCCACA
    GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTTTC CTCAGACATC
    AACAACATGC CCCTGCAAGA GCTGTCTAAT GCCCTTATGG CTGCTGAGCG CTTCAAGGAG
    CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCT
    CCTACTCTCC CACGTTCTAA CACAGACTCG GCCGTGGTTC TTGAGGAGGG AAGTCCAGGA
    GAGGCTCATG TGCCAGTGGA GCCCCCCGAA TTGGAGGACT TTGAGGCAGC ATTAGGTACA
    GACAGGCACT GCCAGCGGCC TGACGCCTAC AGCAGGTCTT CCTCAGCATC TAGCCAGGAA
    GAAAAGTCCC ACTTGGAGGA GCTGGCTGCA GGGGGTATCC CCATTGAGCG GGCCATCTCC
    TCTGAGGGGG CTCGGCCTAC AGTGGACCTG TCCTTCCAAC CCTCACAGCC TTTGAGCAAG
    TCTAGCTCTT CTCCTGAGTT ACAGACCTTA CAGGACATCC TTGGAGACCT AGGGGACAAG
    ACTGACATTG GACGGCTGAG TCCTGAGGCT AAGGTCCGAT CCCAGTCGGG GATCCTGGAT
    GGGGAAGCAG CTACCTGGTC AGCTCCGGGT GAAGAGAGCC GTATCACAGT CCCACCTGAA
    GGTCCCCTGC CTTCCAGTTC TCCCCGCTCC CCTAGTGGCC TCCGGCCCCG AGGCTATACC
    ATCTCTGATT CAGCTCCATC ACGAAGGGGA AAGAGGGTAG AAAGGGACAA CTTCAAGAGC
    AGAACTGCGG CCTCCAGTGC TGAAAAGGTG CCAGGCATCA ACCCTAGCTT TGTGTTCCTA
    CAGCTCTACC ATTCACCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT GTTGCCCAAC
    GAGTCCTTTG AACGGTCAGT GCAGCTCCTT GACCAGATCC CATCCTATGA CACTCACAAG
    ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAGCAGTG AGCTGGCCAT CCTATCAAAT
    GAGCATGGCT CTTATAGGTA CACAGAGTTT CTGACAGGCC TGGGTCGGCT TATTGAGCTG
    AAGGACTGCC AACCAGACAA GGTGTACTTA GGTGGACTGG ATGTGTGTGG CGAGGATGGA
    CAGTTCACGT ACTGCTGGCA TGATGACATC ATGCAAGCTG TTTTCCACAT TGCTACCCTG
    ATGCCCACCA AGGATGTGGA CAAGCACCGT TGTGACAAGA AACGGCACCT GGGCAATGAC
    TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AGCTGGGCAC CATTAAGGGC
    CAGTTCAACT TCGTCCATGT GATCATCACA CCGCTGGACT ATAAATGCAA CCTGTTGACC
    CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GTGTGGCCAA GATTGTGTCT
    GACCGCAACC TGTCCTTTGT GGCCCGACAG ATGGCCCTGC ATGCAAATAT GGCCTCACAG
    GTACACCACA GCCGATCCAA CCCCACGGAC ATCTATCCCT CCAAGTGGAT CGCAAGACTC
    CGCCACATTA AGCGTCTCCG TCAGCGGATC CGTGAAGAAG TGCACTATTC AAACCCAAGC
    TTGCCTCTGA TGCACCCTCC AGCCCATACC AAAGTCCCAG CTCAAGCCCC TACGGAGGCT
    ACACCCACCT ACGAGACAGG CCAGCGGAAA CGCCTCATTT CCTCTGTGGA TGACTTCACA
    GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452514.1
    CDS202..5433
    Translation

    Target ORF information:

    RefSeq Version XM_017597025.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGCAG AATACAGACA TCTCTTACCA GTGCCAGCTT GGGGTCTGCG
    GATGAGAACT CTATGGCCCA GGCTGATGAC AACCTGAAGA ATCTCCACTT GGAACTCACA
    GAAACATGTC TGGACATGAT GGCCCGATAT GTGTTCTCCA ACTTCACTGC GGTTCCCAAG
    AGGTCCCCTG TGGGAGAATT CCTCCTGGCA GGGGGCAGGA CCAAAACGTG GCTGGTTGGA
    AATAAGCTGG TCACTGTGAC AACGAGTGTG GGAACTGGTA CGCGGTCGTT GCTGGGCCTG
    GACTCCGGGG ATCTGCAGGG TGGCTCAGCA TCAAGCTCTG ATCCTGGCAC ACATGTGAGG
    CAGACAAAGG AAGCACCGGC CAAACTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG
    GCCCGGGACC GAGTCCGCTC CATGTCAGGG GGCCACGGCC TTCGAGTTGG TGTCCTGGAC
    ACTTCAGCTC CCTACACCCC AGGAGGCCCT GCTTCTCTGG GAGCACAGGC AGCACCAGCA
    GCAAGGCCTG AGAAGCCCTG TGCAGGAGCC CAACTTCCGG CAGCGGAGAA GGCGAACCTG
    GCAGCCTATG TGCCTTTGCT GACCCAGGGC TGGGCAGAGA TCCTGGTCCG CAGGCCCACA
    GGAAACACCA GCTGGCTGAT GAGCTTGGAG AACCCGCTCA GCCCCTTTTC CTCAGACATC
    AACAACATGC CCCTGCAAGA GCTGTCTAAT GCCCTTATGG CTGCTGAGCG CTTCAAGGAG
    CACCGGGACA CGGCCCTGTA CAAGTCATTG TCAGTGCCAG CGGCTGGCAC AGCCAAGCCT
    CCTACTCTCC CACGTTCTAA CACAGACTCG GCCGTGGTTC TTGAGGAGGG AAGTCCAGGA
    GAGGCTCATG TGCCAGTGGA GCCCCCCGAA TTGGAGGACT TTGAGGCAGC ATTAGGTACA
    GACAGGCACT GCCAGCGGCC TGACGCCTAC AGCAGGTCTT CCTCAGCATC TAGCCAGGAA
    GAAAAGTCCC ACTTGGAGGA GCTGGCTGCA GGGGGTATCC CCATTGAGCG GGCCATCTCC
    TCTGAGGGGG CTCGGCCTAC AGTGGACCTG TCCTTCCAAC CCTCACAGCC TTTGAGCAAG
    TCTAGCTCTT CTCCTGAGTT ACAGACCTTA CAGGACATCC TTGGAGACCT AGGGGACAAG
    ACTGACATTG GACGGCTGAG TCCTGAGGCT AAGGTCCGAT CCCAGTCGGG GATCCTGGAT
    GGGGAAGCAG CTACCTGGTC AGCTCCGGGT GAAGAGAGCC GTATCACAGT CCCACCTGAA
    GGTCCCCTGC CTTCCAGTTC TCCCCGCTCC CCTAGTGGCC TCCGGCCCCG AGGCTATACC
    ATCTCTGATT CAGCTCCATC ACGAAGGGGA AAGAGGGTAG AAAGGGACAA CTTCAAGAGC
    AGAACTGCGG CCTCCAGTGC TGAAAAGGTG CCAGGCATCA ACCCTAGCTT TGTGTTCCTA
    CAGCTCTACC ATTCACCCTT TTTTGGTGAT GAGTCCAATA AGCCCATCCT GTTGCCCAAC
    GAGTCCTTTG AACGGTCAGT GCAGCTCCTT GACCAGATCC CATCCTATGA CACTCACAAG
    ATTGCTGTCC TGTATGTGGG AGAAGGCCAG AGCAGCAGTG AGCTGGCCAT CCTATCAAAT
    GAGCATGGCT CTTATAGGTA CACAGAGTTT CTGACAGGCC TGGGTCGGCT TATTGAGCTG
    AAGGACTGCC AACCAGACAA GGTGTACTTA GGTGGACTGG ATGTGTGTGG CGAGGATGGA
    CAGTTCACGT ACTGCTGGCA TGATGACATC ATGCAAGCTG TTTTCCACAT TGCTACCCTG
    ATGCCCACCA AGGATGTGGA CAAGCACCGT TGTGACAAGA AACGGCACCT GGGCAATGAC
    TTTGTTTCTA TCATCTACAA TGATTCTGGT GAGGACTTCA AGCTGGGCAC CATTAAGGGC
    CAGTTCAACT TCGTCCATGT GATCATCACA CCGCTGGACT ATAAATGCAA CCTGTTGACC
    CTGCAGTGCA GGAAAGACAT GGAGGGCCTT GTGGACACCA GTGTGGCCAA GATTGTGTCT
    GACCGCAACC TGTCCTTTGT GGCCCGACAG ATGGCCCTGC ATGCAAATAT GGCCTCACAG
    GTACACCACA GCCGATCCAA CCCCACGGAC ATCTATCCCT CCAAGTGGAT CGCAAGACTC
    CGCCACATTA AGCGTCTCCG TCAGCGGATC CGTGAAGAAG TGCACTATTC AAACCCAAGC
    TTGCCTCTGA TGCACCCTCC AGCCCATACC AAAGTCCCAG CTCAAGCCCC TACGGAGGCT
    ACACCCACCT ACGAGACAGG CCAGCGGAAA CGCCTCATTT CCTCTGTGGA TGACTTCACA
    GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57188
    Clone ID Related Accession (Same CDS sequence) XM_017597026.1
    Accession Version XM_017597026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGAAT ACAGACATCT CTTACCAGTG CCAGCTTGGG GTCTGCGGAT
    GAGAACTCTA TGGCCCAGGC TGATGACAAC CTGAAGAATC TCCACTTGGA ACTCACAGAA
    ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAACT TCACTGCGGT TCCCAAGAGG
    TCCCCTGTGG GAGAATTCCT CCTGGCAGGG GGCAGGACCA AAACGTGGCT GGTTGGAAAT
    AAGCTGGTCA CTGTGACAAC GAGTGTGGGA ACTGGTACGC GGTCGTTGCT GGGCCTGGAC
    TCCGGGGATC TGCAGGGTGG CTCAGCATCA AGCTCTGATC CTGGCACACA TGTGAGGCAG
    ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGAG TCCGCTCCAT GTCAGGGGGC CACGGCCTTC GAGTTGGTGT CCTGGACACT
    TCAGCTCCCT ACACCCCAGG AGGCCCTGCT TCTCTGGGAG CACAGGCAGC ACCAGCAGCA
    AGGCCTGAGA AGCCCTGTGC AGGAGCCCAA CTTCCGGCAG CGGAGAAGGC GAACCTGGCA
    GCCTATGTGC CTTTGCTGAC CCAGGGCTGG GCAGAGATCC TGGTCCGCAG GCCCACAGGA
    AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTTTCCTC AGACATCAAC
    AACATGCCCC TGCAAGAGCT GTCTAATGCC CTTATGGCTG CTGAGCGCTT CAAGGAGCAC
    CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCTCCT
    ACTCTCCCAC GTTCTAACAC AGACTCGGCC GTGGTTCTTG AGGAGGGAAG TCCAGGAGAG
    GCTCATGTGC CAGTGGAGCC CCCCGAATTG GAGGACTTTG AGGCAGCATT AGGTACAGAC
    AGGCACTGCC AGCGGCCTGA CGCCTACAGC AGGTCTTCCT CAGCATCTAG CCAGGAAGAA
    AAGTCCCACT TGGAGGAGCT GGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT
    GAGGGGGCTC GGCCTACAGT GGACCTGTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT
    AGCTCTTCTC CTGAGTTACA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGACT
    GACATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGATCCC AGTCGGGGAT CCTGGATGGG
    GAAGCAGCTA CCTGGTCAGC TCCGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT
    CCCCTGCCTT CCAGTTCTCC CCGCTCCCCT AGTGGCCTCC GGCCCCGAGG CTATACCATC
    TCTGATTCAG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGACAACTT CAAGAGCAGA
    ACTGCGGCCT CCAGTGCTGA AAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG
    CTCTACCATT CACCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTGTT GCCCAACGAG
    TCCTTTGAAC GGTCAGTGCA GCTCCTTGAC CAGATCCCAT CCTATGACAC TCACAAGATT
    GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT ATCAAATGAG
    CATGGCTCTT ATAGGTACAC AGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTGAAG
    GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TGTGTGGCGA GGATGGACAG
    TTCACGTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC TACCCTGATG
    CCCACCAAGG ATGTGGACAA GCACCGTTGT GACAAGAAAC GGCACCTGGG CAATGACTTT
    GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAGC TGGGCACCAT TAAGGGCCAG
    TTCAACTTCG TCCATGTGAT CATCACACCG CTGGACTATA AATGCAACCT GTTGACCCTG
    CAGTGCAGGA AAGACATGGA GGGCCTTGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC
    CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA
    CACCACAGCC GATCCAACCC CACGGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC
    CACATTAAGC GTCTCCGTCA GCGGATCCGT GAAGAAGTGC ACTATTCAAA CCCAAGCTTG
    CCTCTGATGC ACCCTCCAGC CCATACCAAA GTCCCAGCTC AAGCCCCTAC GGAGGCTACA
    CCCACCTACG AGACAGGCCA GCGGAAACGC CTCATTTCCT CTGTGGATGA CTTCACAGAG
    TTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452515.1
    CDS202..5430
    Translation

    Target ORF information:

    RefSeq Version XM_017597026.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TCATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAC
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACCTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGAAT ACAGACATCT CTTACCAGTG CCAGCTTGGG GTCTGCGGAT
    GAGAACTCTA TGGCCCAGGC TGATGACAAC CTGAAGAATC TCCACTTGGA ACTCACAGAA
    ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAACT TCACTGCGGT TCCCAAGAGG
    TCCCCTGTGG GAGAATTCCT CCTGGCAGGG GGCAGGACCA AAACGTGGCT GGTTGGAAAT
    AAGCTGGTCA CTGTGACAAC GAGTGTGGGA ACTGGTACGC GGTCGTTGCT GGGCCTGGAC
    TCCGGGGATC TGCAGGGTGG CTCAGCATCA AGCTCTGATC CTGGCACACA TGTGAGGCAG
    ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGAG TCCGCTCCAT GTCAGGGGGC CACGGCCTTC GAGTTGGTGT CCTGGACACT
    TCAGCTCCCT ACACCCCAGG AGGCCCTGCT TCTCTGGGAG CACAGGCAGC ACCAGCAGCA
    AGGCCTGAGA AGCCCTGTGC AGGAGCCCAA CTTCCGGCAG CGGAGAAGGC GAACCTGGCA
    GCCTATGTGC CTTTGCTGAC CCAGGGCTGG GCAGAGATCC TGGTCCGCAG GCCCACAGGA
    AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTTTCCTC AGACATCAAC
    AACATGCCCC TGCAAGAGCT GTCTAATGCC CTTATGGCTG CTGAGCGCTT CAAGGAGCAC
    CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCTCCT
    ACTCTCCCAC GTTCTAACAC AGACTCGGCC GTGGTTCTTG AGGAGGGAAG TCCAGGAGAG
    GCTCATGTGC CAGTGGAGCC CCCCGAATTG GAGGACTTTG AGGCAGCATT AGGTACAGAC
    AGGCACTGCC AGCGGCCTGA CGCCTACAGC AGGTCTTCCT CAGCATCTAG CCAGGAAGAA
    AAGTCCCACT TGGAGGAGCT GGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT
    GAGGGGGCTC GGCCTACAGT GGACCTGTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT
    AGCTCTTCTC CTGAGTTACA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGACT
    GACATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGATCCC AGTCGGGGAT CCTGGATGGG
    GAAGCAGCTA CCTGGTCAGC TCCGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT
    CCCCTGCCTT CCAGTTCTCC CCGCTCCCCT AGTGGCCTCC GGCCCCGAGG CTATACCATC
    TCTGATTCAG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGACAACTT CAAGAGCAGA
    ACTGCGGCCT CCAGTGCTGA AAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG
    CTCTACCATT CACCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTGTT GCCCAACGAG
    TCCTTTGAAC GGTCAGTGCA GCTCCTTGAC CAGATCCCAT CCTATGACAC TCACAAGATT
    GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT ATCAAATGAG
    CATGGCTCTT ATAGGTACAC AGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTGAAG
    GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TGTGTGGCGA GGATGGACAG
    TTCACGTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC TACCCTGATG
    CCCACCAAGG ATGTGGACAA GCACCGTTGT GACAAGAAAC GGCACCTGGG CAATGACTTT
    GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAGC TGGGCACCAT TAAGGGCCAG
    TTCAACTTCG TCCATGTGAT CATCACACCG CTGGACTATA AATGCAACCT GTTGACCCTG
    CAGTGCAGGA AAGACATGGA GGGCCTTGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC
    CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA
    CACCACAGCC GATCCAACCC CACGGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC
    CACATTAAGC GTCTCCGTCA GCGGATCCGT GAAGAAGTGC ACTATTCAAA CCCAAGCTTG
    CCTCTGATGC ACCCTCCAGC CCATACCAAA GTCCCAGCTC AAGCCCCTAC GGAGGCTACA
    CCCACCTACG AGACAGGCCA GCGGAAACGC CTCATTTCCT CTGTGGATGA CTTCACAGAG
    TTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57189
    Clone ID Related Accession (Same CDS sequence) XM_017597027.1
    Accession Version XM_017597027.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTCGTG
    AACCTGGTCA AGTTCAACAG CTGCTACCTT GACGAATACA TTGCGCCAAT GGTTCACATG
    ATTTGTCTGT TATGTATCCG GACAGTGTCC TCTGTGGACA TCGAGGTGTC CTTGCAAGTG
    CTGGATGCTG TGGTCTGCTA CAACTGCCTG CCTGCTGAGA GCCTGCCTCT GTTCATTATC
    ACCCTGTGCC GCACCGTCAA CGTCAAGGAG CTGTGTGAGC CGTGCTGGAA GCTGATGCGA
    AACCTTCTAG GTACCCATCT AGGCCACAGT GCCATTTACA ACATGTGCCG TATCATGGAG
    AACAGATCCT ACATGGAAGA CGCCCCCTTG TTGAGGGGAG CCGTGTTCTT TGTTGGGATG
    GCGCTCTGGG GAGCTCACCG ACTCTATTCG CTCAAGAACT CCCCCACATC TGTGCTGCCG
    TCTTTTTATG AGGCCATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC
    ATAACAAGAC TCATCAAGAA GTACAGGAAG GAGCTCCAGG CTGTGACATG GGACATTCTG
    CTGGACATCA TTGAACGACT GCTCCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAGG
    ACCATTGTCC ATGACCTGCT GACCACTGTG GAGGAGCTGT GTGACCAGAA CGAGTTCCAC
    GGATCGCAAG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG GCCTGAATCC
    TCTCTCTTAA ACCTGATAAC CTACAGAGCC CAGTCTATCC ACCCAGCCAA GGATGGCTGG
    ATCCAGAACC TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ACGAGTGCCG CAGTGCCGTG
    CGCATCAAGG TGCTGGACGT GCTGTCCTTC GTGCTCCTCA TCAACAGGCA GTTCTACGAG
    GAGGAGCTGA TTAACTCGGT GGTCATCTCA CAGCTCTCCC ACATCCCCGA GGATAAAGAC
    CATCAGGTTC GAAAGCTGGC TACCCAGCTG CTGGTGGACC TGGCAGAGGG CTGCCACACC
    CACCACTTCA ACAGTCTGTT GGACATCATT GAAAAGGTGA TGGCTCGCTC CCTTTCTCCA
    CCCCTGGAGC TGGAGGAAAG GGATCTGGCT GTGTACTCGG CCTCCCTGGA GGATGTGAAG
    ACGGCAGTCC TCGGGCTCCT GGTCATCCTT CAGACCAAGC TGTATACCTT GCCCGCCAGC
    CATGCCACAC GAGTGTATGA GACACTCATT AGCCACATCC AGCTCCATTA CAAGCACGGC
    TACTCCTTGC CCATTGCTAG CAGCATCCGG CTGCAGGCCT TTGACTTCCT GCTGCTACTG
    CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GCGATTCAGC
    CCTTACTGCC TCTGTGACTG CGCGGAACTG GACAGGGCCT CAGAGAAGAA AGCCAGCGGA
    CCCCTTTCAC CGCCAACTGG GCCCCCCAGC CCTGTGCCAA CGGGCCCTGC TGTGAGGCTC
    GGCCACCTGC CCTACTCCCT GCTTTTCCGT GTCCTGCTGC AGTGTTTGAA GCAGGAGACC
    GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AAACTCCCTG AGTCGCTGCG CTACAAAGTC
    CTCATTTTCA CCTCCCCCTG CAGTGTTGAC CAGCTGTCTT CTGCTCTCTG CTCCATGCTT
    TCAGCCCCGA AGACCCTTGA GCGGCTCCGA GGCACCCCAG AAGGCTTCTC CAGAACTGAC
    CTGCACTTGG CTGTGGTCCC TGTACTGACA GCGTTAATAT CTTACCACAA CTATTTGGAT
    AAGACTAGAC AGCGTGAGAT GGTTTACTGC CTAGAACAAG GCCTTATCTA CCGCTGTGCC
    AGCCAGTGTG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG
    GCGCTCCCTG TCCTGGTGGT GAAGCTGACA CACATCTCTG CTACAGCTAG CATGGCCATC
    CCCCTCCTGG AGTTCCTGTC TACCTTGGCC AGGCTGCCTC ACCTCTACAG GAACTTCGCC
    GCAGAGCAGT ATGCAAGTGT GTTTGCCATC TCCTTGCCCT ACACCAACCC CTCCAAGTTC
    AACCAGTACA TTGTGTGTCT GGCCCATCAC GTCATAGCCA TGTGGTTCAT TAGGTGCCGA
    CTGCCCTTTC GGAAGGATTT TGTCCCTTAT ATCACTAAGG GTCTGCGTTC CAATGTCCTC
    CTGTCCTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT
    GAGAGACCCA AAAGCAGAAT ACAGACATCT CTTACCAGTG CCAGCTTGGG GTCTGCGGAT
    GAGAACTCTA TGGCCCAGGC TGATGACAAC CTGAAGAATC TCCACTTGGA ACTCACAGAA
    ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAACT TCACTGCGGT TCCCAAGAGG
    TCCCCTGTGG GAGAATTCCT CCTGGCAGGG GGCAGGACCA AAACGTGGCT GGTTGGAAAT
    AAGCTGGTCA CTGTGACAAC GAGTGTGGGA ACTGGTACGC GGTCGTTGCT GGGCCTGGAC
    TCCGGGGATC TGCAGGGTGG CTCAGCATCA AGCTCTGATC CTGGCACACA TGTGAGGCAG
    ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGAG TCCGCTCCAT GTCAGGGGGC CACGGCCTTC GAGTTGGTGT CCTGGACACT
    TCAGCTCCCT ACACCCCAGG AGGCCCTGCT TCTCTGGGAG CACAGGCAGC ACCAGCAGCA
    AGGCCTGAGA AGCCCTGTGC AGGAGCCCAA CTTCCGGCAG CGGAGAAGGC GAACCTGGCA
    GCCTATGTGC CTTTGCTGAC CCAGGGCTGG GCAGAGATCC TGGTCCGCAG GCCCACAGGA
    AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTTTCCTC AGACATCAAC
    AACATGCCCC TGCAAGAGCT GTCTAATGCC CTTATGGCTG CTGAGCGCTT CAAGGAGCAC
    CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCTCCT
    ACTCTCCCAC GTTCTAACAC AGACTCGGCC GTGGTTCTTG AGGAGGGAAG TCCAGGAGAG
    GCTCATGTGC CAGTGGAGCC CCCCGAATTG GAGGACTTTG AGGCAGCATT AGGTACAGAC
    AGGCACTGCC AGCGGCCTGA CGCCTACAGC AGGTCTTCCT CAGCATCTAG CCAGGAAGAA
    AAGTCCCACT TGGAGGAGCT GGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT
    GAGGGGGCTC GGCCTACAGT GGACCTGTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT
    AGCTCTTCTC CTGAGTTACA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGACT
    GACATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGATCCC AGTCGGGGAT CCTGGATGGG
    GAAGCAGCTA CCTGGTCAGC TCCGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT
    CCCCTGCCTT CCAGTTCTCC CCGCTCCCCT AGTGGCCTCC GGCCCCGAGG CTATACCATC
    TCTGATTCAG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGACAACTT CAAGAGCAGA
    ACTGCGGCCT CCAGTGCTGA AAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG
    CTCTACCATT CACCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTGTT GCCCAACGAG
    TCCTTTGAAC GGTCAGTGCA GCTCCTTGAC CAGATCCCAT CCTATGACAC TCACAAGATT
    GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT ATCAAATGAG
    CATGGCTCTT ATAGGTACAC AGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTGAAG
    GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TGTGTGGCGA GGATGGACAG
    TTCACGTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC TACCCTGATG
    CCCACCAAGG ATGTGGACAA GCACCGTTGT GACAAGAAAC GGCACCTGGG CAATGACTTT
    GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAGC TGGGCACCAT TAAGGGCCAG
    TTCAACTTCG TCCATGTGAT CATCACACCG CTGGACTATA AATGCAACCT GTTGACCCTG
    CAGTGCAGGA AAGACATGGA GGGCCTTGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC
    CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA
    CACCACAGCC GATCCAACCC CACGGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC
    CACATTAAGC GTCTCCGTCA GCGGATCCGT GAAGAAGTGC ACTATTCAAA CCCAAGCTTG
    CCTCTGATGC ACCCTCCAGC CCATACCAAA GTCCCAGCTC AAGCCCCTAC GGAGGCTACA
    CCCACCTACG AGACAGGCCA GCGGAAACGC CTCATTTCCT CTGTGGATGA CTTCACAGAG
    TTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017452516.1
    CDS202..5430
    Translation

    Target ORF information:

    RefSeq Version XM_017597027.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GAGTTTGTCC TGCAGTGGAT GGATGTTGGC TTGTCCTCAG AATTCCTTCT GGTACTCGTG
    AACCTGGTCA AGTTCAACAG CTGCTACCTT GACGAATACA TTGCGCCAAT GGTTCACATG
    ATTTGTCTGT TATGTATCCG GACAGTGTCC TCTGTGGACA TCGAGGTGTC CTTGCAAGTG
    CTGGATGCTG TGGTCTGCTA CAACTGCCTG CCTGCTGAGA GCCTGCCTCT GTTCATTATC
    ACCCTGTGCC GCACCGTCAA CGTCAAGGAG CTGTGTGAGC CGTGCTGGAA GCTGATGCGA
    AACCTTCTAG GTACCCATCT AGGCCACAGT GCCATTTACA ACATGTGCCG TATCATGGAG
    AACAGATCCT ACATGGAAGA CGCCCCCTTG TTGAGGGGAG CCGTGTTCTT TGTTGGGATG
    GCGCTCTGGG GAGCTCACCG ACTCTATTCG CTCAAGAACT CCCCCACATC TGTGCTGCCG
    TCTTTTTATG AGGCCATGAC CTGTCCCAAT GAGGTGGTGT CATATGAGAT TGTTCTGTCC
    ATAACAAGAC TCATCAAGAA GTACAGGAAG GAGCTCCAGG CTGTGACATG GGACATTCTG
    CTGGACATCA TTGAACGACT GCTCCAGCAA CTCCAGAACC TGGACAGCCC GGAACTCAGG
    ACCATTGTCC ATGACCTGCT GACCACTGTG GAGGAGCTGT GTGACCAGAA CGAGTTCCAC
    GGATCGCAAG AAAGATACTA TGAACTGGTG GAGAGCTATG CAGACCAGAG GCCTGAATCC
    TCTCTCTTAA ACCTGATAAC CTACAGAGCC CAGTCTATCC ACCCAGCCAA GGATGGCTGG
    ATCCAGAACC TGCAGTTGTT GATGGAGAGG TTCTTCAGGA ACGAGTGCCG CAGTGCCGTG
    CGCATCAAGG TGCTGGACGT GCTGTCCTTC GTGCTCCTCA TCAACAGGCA GTTCTACGAG
    GAGGAGCTGA TTAACTCGGT GGTCATCTCA CAGCTCTCCC ACATCCCCGA GGATAAAGAC
    CATCAGGTTC GAAAGCTGGC TACCCAGCTG CTGGTGGACC TGGCAGAGGG CTGCCACACC
    CACCACTTCA ACAGTCTGTT GGACATCATT GAAAAGGTGA TGGCTCGCTC CCTTTCTCCA
    CCCCTGGAGC TGGAGGAAAG GGATCTGGCT GTGTACTCGG CCTCCCTGGA GGATGTGAAG
    ACGGCAGTCC TCGGGCTCCT GGTCATCCTT CAGACCAAGC TGTATACCTT GCCCGCCAGC
    CATGCCACAC GAGTGTATGA GACACTCATT AGCCACATCC AGCTCCATTA CAAGCACGGC
    TACTCCTTGC CCATTGCTAG CAGCATCCGG CTGCAGGCCT TTGACTTCCT GCTGCTACTG
    CGGGCTGACT CGCTGCATCG ACTGGGCCTG CCCAACAAGG ATGGGGTCGT GCGATTCAGC
    CCTTACTGCC TCTGTGACTG CGCGGAACTG GACAGGGCCT CAGAGAAGAA AGCCAGCGGA
    CCCCTTTCAC CGCCAACTGG GCCCCCCAGC CCTGTGCCAA CGGGCCCTGC TGTGAGGCTC
    GGCCACCTGC CCTACTCCCT GCTTTTCCGT GTCCTGCTGC AGTGTTTGAA GCAGGAGACC
    GACTGGAAGG TGCTGAAGCT GGTGCTCAGC AAACTCCCTG AGTCGCTGCG CTACAAAGTC
    CTCATTTTCA CCTCCCCCTG CAGTGTTGAC CAGCTGTCTT CTGCTCTCTG CTCCATGCTT
    TCAGCCCCGA AGACCCTTGA GCGGCTCCGA GGCACCCCAG AAGGCTTCTC CAGAACTGAC
    CTGCACTTGG CTGTGGTCCC TGTACTGACA GCGTTAATAT CTTACCACAA CTATTTGGAT
    AAGACTAGAC AGCGTGAGAT GGTTTACTGC CTAGAACAAG GCCTTATCTA CCGCTGTGCC
    AGCCAGTGTG TGGTAGCCTT GGCCATCTGC AGTGTGGAGA TGCCCGACAT CATCATCAAG
    GCGCTCCCTG TCCTGGTGGT GAAGCTGACA CACATCTCTG CTACAGCTAG CATGGCCATC
    CCCCTCCTGG AGTTCCTGTC TACCTTGGCC AGGCTGCCTC ACCTCTACAG GAACTTCGCC
    GCAGAGCAGT ATGCAAGTGT GTTTGCCATC TCCTTGCCCT ACACCAACCC CTCCAAGTTC
    AACCAGTACA TTGTGTGTCT GGCCCATCAC GTCATAGCCA TGTGGTTCAT TAGGTGCCGA
    CTGCCCTTTC GGAAGGATTT TGTCCCTTAT ATCACTAAGG GTCTGCGTTC CAATGTCCTC
    CTGTCCTTTG ATGATACCCC TGAGAAGGAC AGTTTCAGAG CACGGAGCAC CAGTCTTAAT
    GAGAGACCCA AAAGCAGAAT ACAGACATCT CTTACCAGTG CCAGCTTGGG GTCTGCGGAT
    GAGAACTCTA TGGCCCAGGC TGATGACAAC CTGAAGAATC TCCACTTGGA ACTCACAGAA
    ACATGTCTGG ACATGATGGC CCGATATGTG TTCTCCAACT TCACTGCGGT TCCCAAGAGG
    TCCCCTGTGG GAGAATTCCT CCTGGCAGGG GGCAGGACCA AAACGTGGCT GGTTGGAAAT
    AAGCTGGTCA CTGTGACAAC GAGTGTGGGA ACTGGTACGC GGTCGTTGCT GGGCCTGGAC
    TCCGGGGATC TGCAGGGTGG CTCAGCATCA AGCTCTGATC CTGGCACACA TGTGAGGCAG
    ACAAAGGAAG CACCGGCCAA ACTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC
    CGGGACCGAG TCCGCTCCAT GTCAGGGGGC CACGGCCTTC GAGTTGGTGT CCTGGACACT
    TCAGCTCCCT ACACCCCAGG AGGCCCTGCT TCTCTGGGAG CACAGGCAGC ACCAGCAGCA
    AGGCCTGAGA AGCCCTGTGC AGGAGCCCAA CTTCCGGCAG CGGAGAAGGC GAACCTGGCA
    GCCTATGTGC CTTTGCTGAC CCAGGGCTGG GCAGAGATCC TGGTCCGCAG GCCCACAGGA
    AACACCAGCT GGCTGATGAG CTTGGAGAAC CCGCTCAGCC CCTTTTCCTC AGACATCAAC
    AACATGCCCC TGCAAGAGCT GTCTAATGCC CTTATGGCTG CTGAGCGCTT CAAGGAGCAC
    CGGGACACGG CCCTGTACAA GTCATTGTCA GTGCCAGCGG CTGGCACAGC CAAGCCTCCT
    ACTCTCCCAC GTTCTAACAC AGACTCGGCC GTGGTTCTTG AGGAGGGAAG TCCAGGAGAG
    GCTCATGTGC CAGTGGAGCC CCCCGAATTG GAGGACTTTG AGGCAGCATT AGGTACAGAC
    AGGCACTGCC AGCGGCCTGA CGCCTACAGC AGGTCTTCCT CAGCATCTAG CCAGGAAGAA
    AAGTCCCACT TGGAGGAGCT GGCTGCAGGG GGTATCCCCA TTGAGCGGGC CATCTCCTCT
    GAGGGGGCTC GGCCTACAGT GGACCTGTCC TTCCAACCCT CACAGCCTTT GAGCAAGTCT
    AGCTCTTCTC CTGAGTTACA GACCTTACAG GACATCCTTG GAGACCTAGG GGACAAGACT
    GACATTGGAC GGCTGAGTCC TGAGGCTAAG GTCCGATCCC AGTCGGGGAT CCTGGATGGG
    GAAGCAGCTA CCTGGTCAGC TCCGGGTGAA GAGAGCCGTA TCACAGTCCC ACCTGAAGGT
    CCCCTGCCTT CCAGTTCTCC CCGCTCCCCT AGTGGCCTCC GGCCCCGAGG CTATACCATC
    TCTGATTCAG CTCCATCACG AAGGGGAAAG AGGGTAGAAA GGGACAACTT CAAGAGCAGA
    ACTGCGGCCT CCAGTGCTGA AAAGGTGCCA GGCATCAACC CTAGCTTTGT GTTCCTACAG
    CTCTACCATT CACCCTTTTT TGGTGATGAG TCCAATAAGC CCATCCTGTT GCCCAACGAG
    TCCTTTGAAC GGTCAGTGCA GCTCCTTGAC CAGATCCCAT CCTATGACAC TCACAAGATT
    GCTGTCCTGT ATGTGGGAGA AGGCCAGAGC AGCAGTGAGC TGGCCATCCT ATCAAATGAG
    CATGGCTCTT ATAGGTACAC AGAGTTTCTG ACAGGCCTGG GTCGGCTTAT TGAGCTGAAG
    GACTGCCAAC CAGACAAGGT GTACTTAGGT GGACTGGATG TGTGTGGCGA GGATGGACAG
    TTCACGTACT GCTGGCATGA TGACATCATG CAAGCTGTTT TCCACATTGC TACCCTGATG
    CCCACCAAGG ATGTGGACAA GCACCGTTGT GACAAGAAAC GGCACCTGGG CAATGACTTT
    GTTTCTATCA TCTACAATGA TTCTGGTGAG GACTTCAAGC TGGGCACCAT TAAGGGCCAG
    TTCAACTTCG TCCATGTGAT CATCACACCG CTGGACTATA AATGCAACCT GTTGACCCTG
    CAGTGCAGGA AAGACATGGA GGGCCTTGTG GACACCAGTG TGGCCAAGAT TGTGTCTGAC
    CGCAACCTGT CCTTTGTGGC CCGACAGATG GCCCTGCATG CAAATATGGC CTCACAGGTA
    CACCACAGCC GATCCAACCC CACGGACATC TATCCCTCCA AGTGGATCGC AAGACTCCGC
    CACATTAAGC GTCTCCGTCA GCGGATCCGT GAAGAAGTGC ACTATTCAAA CCCAAGCTTG
    CCTCTGATGC ACCCTCCAGC CCATACCAAA GTCCCAGCTC AAGCCCCTAC GGAGGCTACA
    CCCACCTACG AGACAGGCCA GCGGAAACGC CTCATTTCCT CTGTGGATGA CTTCACAGAG
    TTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa00599
    Clone ID Related Accession (Same CDS sequence) NM_012680.3
    Accession Version NM_012680.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Rattus norvegicus(Norway rat)
    Product tuberin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U24150.1. On Feb 21, 2013 this sequence version replaced NM_012680.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U24150.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TTATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAT
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAAGTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT
    CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CTCTTACCAG TGCCAGCTTG
    GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACCTGAAGAA TCTCCACTTG
    GAACTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA CTTCACTGCG
    GTTCCCAAGA GGTCCCCTGT GGGAGAATTC CTCCTGGCAG GGGGCAGGAC CAAAACGTGG
    CTGGTTGGAA ATAAGCTGGT CACTGTGACA ACGAGTGTGG GAACTGGTAC GCGGTCGTTG
    CTGGGCCTGG ACTCCGGGGA TCTGCAGGGT GGCTCAGCAT CAAGCTCTGA TCCTGGCACA
    CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG AGTCCGCTCC ATGTCAGGGG GCCACGGCCT TCGAGTTGGT
    GTCCTGGACA CTTCAGCTCC CTACACCCCA GGAGGCCCTG CTTCTCTGGG AGCACAGGCA
    GCACCAGCAG CAAGGCCTGA GAAGCCCTGT GCAGGAGCCC AACTTCCGGC AGCGGAGAAG
    GCGAACCTGG CAGCCTATGT GCCTTTGCTG ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC
    AGGCCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTTTCC
    TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCTAATG CCCTTATGGC TGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC GGCCCTATAC AAGTCATTGT CAGTGCCAGC GGCTGGCACA
    GCCAAGCCTC CTACTCTCCC ACGTTCTAAC ACAGTGGCTT CTTTCTCCTC CCTGTACCAG
    CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCGGC CGTGGTTCTT
    GAGGAGGGAA GTCCAGGAGA GGCTCATGTG CCAGTGGAGC CCCCCGAATT GGAGGACTTT
    GAGGCAGCAT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACGCCTACAG CAGGTCTTCC
    TCAGCATCTA GCCAGGAAGA AAAGTCCCAC TTGGAGGAGC TGGCTGCAGG GGGTATCCCC
    ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTACAG TGGACCTGTC CTTCCAACCC
    TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCTGAGTTAC AGACCTTACA GGACATCCTT
    GGAGACCTAG GGGACAAGAC TGACATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGATCC
    CAGTCGGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTCCGGGTGA AGAGAGCCGT
    ATCACAGTCC CACCTGAAGG TCCCCTGCCT TCCAGTTCTC CCCGCTCCCC TAGTGGCCTC
    CGGCCCCGAG GCTATACCAT CTCTGATTCA GCTCCATCAC GAAGGGGAAA GAGGGTAGAA
    AGGGACAACT TCAAGAGCAG AACTGCGGCC TCCAGTGCTG AAAAGGTGCC AGGCATCAAC
    CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCACCCTTTT GTGGTGATGA GTCCAATAAG
    CCCATCCTGT TGCCCAACGA GTCCTTTGAA CGGTCAGTGC AGCTCCTTGA CCAGATCCCA
    TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG
    CTGGCCATCC TATCAAATGA GCATGGCTCT TATAGGTACA CAGAGTTTCT GACAGGCCTG
    GGTCGGCTTA TTGAGCTGAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT
    GTGTGTGGCG AGGATGGACA GTTCACGTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT
    TTCCACATTG CTACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGTTG TGACAAGAAA
    CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAG
    CTGGGCACCA TTAAGGGCCA GTTCAACTTC GTCCATGTGA TCATCACACC GCTGGACTAT
    AAATGCAACC TGTTGACCCT GCAGTGCAGG AAAGACATGG AGGGCCTTGT GGACACCAGT
    GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT
    GCAAATATGG CCTCACAGGT ACACCACAGG CGATCCAACC CCACGGACAT CTATCCCTCC
    AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGTC AGCGGATCCG TGAAGAAGTG
    CACTATTCAA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCATACCAA AGTCCCAGCT
    CAAGCCCCTA CGGAGGCTAC ACCCACCTAC GAGACAGGCC AGCGGAAACG CCTCATTTCC
    TCTGTGGATG ACTTCACAGA GTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036812.2
    CDS84..5513
    Translation

    Target ORF information:

    RefSeq Version NM_012680.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus TSC complex subunit 2 (Tsc2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_012680.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAAC CAACTAGCAA AGATTCAGGT TTGAAGGAGA AGTTCAAGAT ACTATTAGGA 
    CTGGGAACGT CAAGGCCAAA TCCTAGGTGT GCAGAAGGCA AACAGACGGA GTTTATCATC
    ACAGCGGAAA TCTTGAGAGA ACTGAGTGGT GAATGCGGCC TCAACAATCG AATCCGGATG
    ATAGGGCAGA TCTGTGATGT GGCAAAAACT AAGAAACTTG AAGAGCATGC AGTGGAGGCA
    CTTTGGAAGG CTGTCTCAGA CTTGCTACAG CCAGAGCGGC CACCGGAGGC CCGACATGCA
    GTTCTGGCCT TGTTGAAGGC CATTGTGCAG GGACAGGGTG ATCGTTTGGG GGTTCTCCGA
    GCCCTCTTCT TCAAAGTGAT CAAGGACTAT CCTTCCAACG AAGACCTCCA TGAAAGGCTA
    GAGGTTTTTA AGGCCCTCAC AGACAATGGA AGGCATATTA CCTACTTGGA AGAAGAACTG
    GCAGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CAGAATTCCT TCTGGTACTC
    GTGAACCTGG TCAAGTTCAA CAGCTGCTAC CTTGACGAAT ACATTGCGCC AATGGTTCAC
    ATGATTTGTC TGTTATGTAT CCGGACAGTG TCCTCTGTGG ACATCGAGGT GTCCTTGCAA
    GTGCTGGATG CTGTGGTCTG CTACAACTGC CTGCCTGCTG AGAGCCTGCC TCTGTTCATT
    ATCACCCTGT GCCGCACCGT CAACGTCAAG GAGCTGTGTG AGCCGTGCTG GAAGCTGATG
    CGAAACCTTC TAGGTACCCA TCTAGGCCAC AGTGCCATTT ACAACATGTG CCGTATCATG
    GAGAACAGAT CCTACATGGA AGACGCCCCC TTGTTGAGGG GAGCCGTGTT CTTTGTTGGG
    ATGGCGCTCT GGGGAGCTCA CCGACTCTAT TCGCTCAAGA ACTCCCCCAC ATCTGTGCTG
    CCGTCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCATATGA GATTGTTCTG
    TCCATAACAA GACTCATCAA GAAGTACAGG AAGGAGCTCC AGGCTGTGAC ATGGGACATT
    CTGCTGGACA TTATTGAACG ACTGCTCCAG CAACTCCAGA ACCTGGACAG CCCGGAACTC
    AGGACCATTG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAACGAGTTC
    CACGGATCGC AAGAAAGATA CTATGAACTG GTGGAGAGCT ATGCAGACCA GAGGCCTGAA
    TCCTCTCTCT TAAACCTGAT AACCTACAGA GCCCAGTCTA TCCACCCAGC CAAGGATGGC
    TGGATCCAGA ACCTGCAGTT GTTGATGGAG AGGTTCTTCA GGAACGAGTG CCGCAGTGCC
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTCC TCATCAACAG GCAGTTCTAC
    GAGGAGGAGC TGATTAACTC GGTGGTCATC TCACAGCTCT CCCACATCCC CGAGGATAAA
    GACCATCAGG TTCGAAAGCT GGCTACCCAG CTGCTGGTGG ACCTGGCAGA GGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATC ATTGAAAAGG TGATGGCTCG CTCCCTTTCT
    CCACCCCTGG AGCTGGAGGA AAGGGATCTG GCTGTGTACT CGGCCTCCCT GGAGGATGTG
    AAGACGGCAG TCCTCGGGCT CCTGGTCATC CTTCAGACCA AGCTGTATAC CTTGCCCGCC
    AGCCATGCCA CACGAGTGTA TGAGACACTC ATTAGCCACA TCCAGCTCCA TTACAAGCAT
    GGCTACTCCT TGCCCATTGC TAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGCTGCTA
    CTGCGGGCTG ACTCGCTGCA TCGACTGGGC CTGCCCAACA AGGATGGGGT CGTGCGATTC
    AGCCCTTACT GCCTCTGTGA CTGCGCGGAA CTGGACAGGG CCTCAGAGAA GAAAGCCAGC
    GGACCCCTTT CACCGCCAAC TGGGCCCCCC AGCCCTGTGC CAACGGGCCC TGCTGTGAGG
    CTCGGCCACC TGCCCTACTC CCTGCTTTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG
    ACCGACTGGA AGGTGCTGAA GCTGGTGCTC AGCAAACTCC CTGAGTCGCT GCGCTACAAA
    GTCCTCATTT TCACCTCCCC CTGCAGTGTT GACCAGCTGT CTTCTGCTCT CTGCTCCATG
    CTTTCAGCCC CGAAGACCCT TGAGCGGCTC CGAGGCACCC CAGAAGGCTT CTCCAGAACT
    GACTTGCACT TGGCTGTGGT CCCTGTACTG ACAGCGTTAA TATCTTACCA CAACTATTTG
    GATAAGACTA GACAGCGTGA GATGGTTTAC TGCCTAGAAC AAGGCCTTAT CTACCGCTGT
    GCCAGCCAGT GTGTGGTAGC CTTGGCCATC TGCAGTGTGG AGATGCCCGA CATCATCATC
    AAGGCGCTCC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCTACAGC TAGCATGGCC
    ATCCCCCTCC TGGAGTTCCT GTCTACCTTG GCCAGGCTGC CTCACCTCTA CAGGAACTTC
    GCCGCAGAGC AGTATGCAAG TGTGTTTGCC ATCTCCTTGC CCTACACCAA CCCCTCCAAG
    TTCAACCAGT ACATTGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATTAGGTGC
    CGACTGCCCT TTCGGAAGGA TTTTGTCCCT TATATCACTA AGGGTCTGCG TTCCAATGTC
    CTCCTGTCCT TTGATGATAC CCCTGAGAAG GACAAGTTCA GAGCACGGAG CACCAGTCTT
    AATGAGAGAC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCCT GAATAACTCT
    CCACCTGTGA AAGAATTCAA AGAGAGCTGT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC
    AGTGTATCTG AACATGTGGT CCGCAGCAGA ATACAGACAT CTCTTACCAG TGCCAGCTTG
    GGGTCTGCGG ATGAGAACTC TATGGCCCAG GCTGATGACA ACCTGAAGAA TCTCCACTTG
    GAACTCACAG AAACATGTCT GGACATGATG GCCCGATATG TGTTCTCCAA CTTCACTGCG
    GTTCCCAAGA GGTCCCCTGT GGGAGAATTC CTCCTGGCAG GGGGCAGGAC CAAAACGTGG
    CTGGTTGGAA ATAAGCTGGT CACTGTGACA ACGAGTGTGG GAACTGGTAC GCGGTCGTTG
    CTGGGCCTGG ACTCCGGGGA TCTGCAGGGT GGCTCAGCAT CAAGCTCTGA TCCTGGCACA
    CATGTGAGGC AGACAAAGGA AGCACCGGCC AAACTGGAGT CCCAGGCTGG GCAGCAGGTG
    TCCCGTGGGG CCCGGGACCG AGTCCGCTCC ATGTCAGGGG GCCACGGCCT TCGAGTTGGT
    GTCCTGGACA CTTCAGCTCC CTACACCCCA GGAGGCCCTG CTTCTCTGGG AGCACAGGCA
    GCACCAGCAG CAAGGCCTGA GAAGCCCTGT GCAGGAGCCC AACTTCCGGC AGCGGAGAAG
    GCGAACCTGG CAGCCTATGT GCCTTTGCTG ACCCAGGGCT GGGCAGAGAT CCTGGTCCGC
    AGGCCCACAG GAAACACCAG CTGGCTGATG AGCTTGGAGA ACCCGCTCAG CCCCTTTTCC
    TCAGACATCA ACAACATGCC CCTGCAAGAG CTGTCTAATG CCCTTATGGC TGCTGAGCGC
    TTCAAGGAGC ACCGGGACAC GGCCCTATAC AAGTCATTGT CAGTGCCAGC GGCTGGCACA
    GCCAAGCCTC CTACTCTCCC ACGTTCTAAC ACAGTGGCTT CTTTCTCCTC CCTGTACCAG
    CCCAGCTGCC AAGGACAGCT GCACAGGAGC GTTTCCTGGG CAGACTCGGC CGTGGTTCTT
    GAGGAGGGAA GTCCAGGAGA GGCTCATGTG CCAGTGGAGC CCCCCGAATT GGAGGACTTT
    GAGGCAGCAT TAGGTACAGA CAGGCACTGC CAGCGGCCTG ACGCCTACAG CAGGTCTTCC
    TCAGCATCTA GCCAGGAAGA AAAGTCCCAC TTGGAGGAGC TGGCTGCAGG GGGTATCCCC
    ATTGAGCGGG CCATCTCCTC TGAGGGGGCT CGGCCTACAG TGGACCTGTC CTTCCAACCC
    TCACAGCCTT TGAGCAAGTC TAGCTCTTCT CCTGAGTTAC AGACCTTACA GGACATCCTT
    GGAGACCTAG GGGACAAGAC TGACATTGGA CGGCTGAGTC CTGAGGCTAA GGTCCGATCC
    CAGTCGGGGA TCCTGGATGG GGAAGCAGCT ACCTGGTCAG CTCCGGGTGA AGAGAGCCGT
    ATCACAGTCC CACCTGAAGG TCCCCTGCCT TCCAGTTCTC CCCGCTCCCC TAGTGGCCTC
    CGGCCCCGAG GCTATACCAT CTCTGATTCA GCTCCATCAC GAAGGGGAAA GAGGGTAGAA
    AGGGACAACT TCAAGAGCAG AACTGCGGCC TCCAGTGCTG AAAAGGTGCC AGGCATCAAC
    CCTAGCTTTG TGTTCCTACA GCTCTACCAT TCACCCTTTT GTGGTGATGA GTCCAATAAG
    CCCATCCTGT TGCCCAACGA GTCCTTTGAA CGGTCAGTGC AGCTCCTTGA CCAGATCCCA
    TCCTATGACA CTCACAAGAT TGCTGTCCTG TATGTGGGAG AAGGCCAGAG CAGCAGTGAG
    CTGGCCATCC TATCAAATGA GCATGGCTCT TATAGGTACA CAGAGTTTCT GACAGGCCTG
    GGTCGGCTTA TTGAGCTGAA GGACTGCCAA CCAGACAAGG TGTACTTAGG TGGACTGGAT
    GTGTGTGGCG AGGATGGACA GTTCACGTAC TGCTGGCATG ATGACATCAT GCAAGCTGTT
    TTCCACATTG CTACCCTGAT GCCCACCAAG GATGTGGACA AGCACCGTTG TGACAAGAAA
    CGGCACCTGG GCAATGACTT TGTTTCTATC ATCTACAATG ATTCTGGTGA GGACTTCAAG
    CTGGGCACCA TTAAGGGCCA GTTCAACTTC GTCCATGTGA TCATCACACC GCTGGACTAT
    AAATGCAACC TGTTGACCCT GCAGTGCAGG AAAGACATGG AGGGCCTTGT GGACACCAGT
    GTGGCCAAGA TTGTGTCTGA CCGCAACCTG TCCTTTGTGG CCCGACAGAT GGCCCTGCAT
    GCAAATATGG CCTCACAGGT ACACCACAGG CGATCCAACC CCACGGACAT CTATCCCTCC
    AAGTGGATCG CAAGACTCCG CCACATTAAG CGTCTCCGTC AGCGGATCCG TGAAGAAGTG
    CACTATTCAA ACCCAAGCTT GCCTCTGATG CACCCTCCAG CCCATACCAA AGTCCCAGCT
    CAAGCCCCTA CGGAGGCTAC ACCCACCTAC GAGACAGGCC AGCGGAAACG CCTCATTTCC
    TCTGTGGATG ACTTCACAGA GTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa42979
    Clone ID Related Accession (Same CDS sequence) XM_008767544.2
    Accession Version XM_008767544.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tuberin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAAC TTGAAGAGCA TGCAGTGGAG 
    GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCGGA GGCCCGACAT
    GCAGTTCTGG CCTTGTTGAA GGCCATTGTG CAGGGACAGG GTGATCGTTT GGGGGTTCTC
    CGAGCCCTCT TCTTCAAAGT GATCAAGGAC TATCCTTCCA ACGAAGACCT CCATGAAAGG
    CTAGAGGTTT TTAAGGCCCT CACAGACAAT GGAAGGCATA TTACCTACTT GGAAGAAGAA
    CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA
    CTCGTGAACC TGGTCAAGTT CAACAGCTGC TACCTTGACG AATACATTGC GCCAATGGTT
    CACATGATTT GTCTGTTATG TATCCGGACA GTGTCCTCTG TGGACATCGA GGTGTCCTTG
    CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCCTGCCTG CTGAGAGCCT GCCTCTGTTC
    ATTATCACCC TGTGCCGCAC CGTCAACGTC AAGGAGCTGT GTGAGCCGTG CTGGAAGCTG
    ATGCGAAACC TTCTAGGTAC CCATCTAGGC CACAGTGCCA TTTACAACAT GTGCCGTATC
    ATGGAGAACA GATCCTACAT GGAAGACGCC CCCTTGTTGA GGGGAGCCGT GTTCTTTGTT
    GGGATGGCGC TCTGGGGAGC TCACCGACTC TATTCGCTCA AGAACTCCCC CACATCTGTG
    CTGCCGTCTT TTTATGAGGC CATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT
    CTGTCCATAA CAAGACTCAT CAAGAAGTAC AGGAAGGAGC TCCAGGCTGT GACATGGGAC
    ATTCTGCTGG ACATCATTGA ACGACTGCTC CAGCAACTCC AGAACCTGGA CAGCCCGGAA
    CTCAGGACCA TTGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTGTGTGA CCAGAACGAG
    TTCCACGGAT CGCAAGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGGCCT
    GAATCCTCTC TCTTAAACCT GATAACCTAC AGAGCCCAGT CTATCCACCC AGCCAAGGAT
    GGCTGGATCC AGAACCTGCA GTTGTTGATG GAGAGGTTCT TCAGGAACGA GTGCCGCAGT
    GCCGTGCGCA TCAAGGTGCT GGACGTGCTG TCCTTCGTGC TCCTCATCAA CAGGCAGTTC
    TACGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCACAGC TCTCCCACAT CCCCGAGGAT
    AAAGACCATC AGGTTCGAAA GCTGGCTACC CAGCTGCTGG TGGACCTGGC AGAGGGCTGC
    CACACCCACC ACTTCAACAG TCTGTTGGAC ATCATTGAAA AGGTGATGGC TCGCTCCCTT
    TCTCCACCCC TGGAGCTGGA GGAAAGGGAT CTGGCTGTGT ACTCGGCCTC CCTGGAGGAT
    GTGAAGACGG CAGTCCTCGG GCTCCTGGTC ATCCTTCAGA CCAAGCTGTA TACCTTGCCC
    GCCAGCCATG CCACACGAGT GTATGAGACA CTCATTAGCC ACATCCAGCT CCATTACAAG
    CACGGCTACT CCTTGCCCAT TGCTAGCAGC ATCCGGCTGC AGGCCTTTGA CTTCCTGCTG
    CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGCGA
    TTCAGCCCTT ACTGCCTCTG TGACTGCGCG GAACTGGACA GGGCCTCAGA GAAGAAAGCC
    AGCGGACCCC TTTCACCGCC AACTGGGCCC CCCAGCCCTG TGCCAACGGG CCCTGCTGTG
    AGGCTCGGCC ACCTGCCCTA CTCCCTGCTT TTCCGTGTCC TGCTGCAGTG TTTGAAGCAG
    GAGACCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAAAC TCCCTGAGTC GCTGCGCTAC
    AAAGTCCTCA TTTTCACCTC CCCCTGCAGT GTTGACCAGC TGTCTTCTGC TCTCTGCTCC
    ATGCTTTCAG CCCCGAAGAC CCTTGAGCGG CTCCGAGGCA CCCCAGAAGG CTTCTCCAGA
    ACTGACCTGC ACTTGGCTGT GGTCCCTGTA CTGACAGCGT TAATATCTTA CCACAACTAT
    TTGGATAAGA CTAGACAGCG TGAGATGGTT TACTGCCTAG AACAAGGCCT TATCTACCGC
    TGTGCCAGCC AGTGTGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC
    ATCAAGGCGC TCCCTGTCCT GGTGGTGAAG CTGACACACA TCTCTGCTAC AGCTAGCATG
    GCCATCCCCC TCCTGGAGTT CCTGTCTACC TTGGCCAGGC TGCCTCACCT CTACAGGAAC
    TTCGCCGCAG AGCAGTATGC AAGTGTGTTT GCCATCTCCT TGCCCTACAC CAACCCCTCC
    AAGTTCAACC AGTACATTGT GTGTCTGGCC CATCACGTCA TAGCCATGTG GTTCATTAGG
    TGCCGACTGC CCTTTCGGAA GGATTTTGTC CCTTATATCA CTAAGGGTCT GCGTTCCAAT
    GTCCTCCTGT CCTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT
    CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC
    TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC
    ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCTCTTAC CAGTGCCAGC
    TTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACCTGAA GAATCTCCAC
    TTGGAACTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAACTTCACT
    GCGGTTCCCA AGAGGTCCCC TGTGGGAGAA TTCCTCCTGG CAGGGGGCAG GACCAAAACG
    TGGCTGGTTG GAAATAAGCT GGTCACTGTG ACAACGAGTG TGGGAACTGG TACGCGGTCG
    TTGCTGGGCC TGGACTCCGG GGATCTGCAG GGTGGCTCAG CATCAAGCTC TGATCCTGGC
    ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG
    GTGTCCCGTG GGGCCCGGGA CCGAGTCCGC TCCATGTCAG GGGGCCACGG CCTTCGAGTT
    GGTGTCCTGG ACACTTCAGC TCCCTACACC CCAGGAGGCC CTGCTTCTCT GGGAGCACAG
    GCAGCACCAG CAGCAAGGCC TGAGAAGCCC TGTGCAGGAG CCCAACTTCC GGCAGCGGAG
    AAGGCGAACC TGGCAGCCTA TGTGCCTTTG CTGACCCAGG GCTGGGCAGA GATCCTGGTC
    CGCAGGCCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTT
    TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCTA ATGCCCTTAT GGCTGCTGAG
    CGCTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC
    ACAGCCAAGC CTCCTACTCT CCCACGTTCT AACACAGTGG CTTCTTTCTC CTCCCTGTAC
    CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC GGCCGTGGTT
    CTTGAGGAGG GAAGTCCAGG AGAGGCTCAT GTGCCAGTGG AGCCCCCCGA ATTGGAGGAC
    TTTGAGGCAG CATTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACGCCTA CAGCAGGTCT
    TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CACTTGGAGG AGCTGGCTGC AGGGGGTATC
    CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTA CAGTGGACCT GTCCTTCCAA
    CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCTGAGT TACAGACCTT ACAGGACATC
    CTTGGAGACC TAGGGGACAA GACTGACATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGA
    TCCCAGTCGG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTCCGGG TGAAGAGAGC
    CGTATCACAG TCCCACCTGA AGGTCCCCTG CCTTCCAGTT CTCCCCGCTC CCCTAGTGGC
    CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCAGCTCCAT CACGAAGGGG AAAGAGGGTA
    GAAAGGGACA ACTTCAAGAG CAGAACTGCG GCCTCCAGTG CTGAAAAGGT GCCAGGCATC
    AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCACCCT TTTTTGGTGA TGAGTCCAAT
    AAGCCCATCC TGTTGCCCAA CGAGTCCTTT GAACGGTCAG TGCAGCTCCT TGACCAGATC
    CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT
    GAGCTGGCCA TCCTATCAAA TGAGCATGGC TCTTATAGGT ACACAGAGTT TCTGACAGGC
    CTGGGTCGGC TTATTGAGCT GAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG
    GATGTGTGTG GCGAGGATGG ACAGTTCACG TACTGCTGGC ATGATGACAT CATGCAAGCT
    GTTTTCCACA TTGCTACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG TTGTGACAAG
    AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC
    AAGCTGGGCA CCATTAAGGG CCAGTTCAAC TTCGTCCATG TGATCATCAC ACCGCTGGAC
    TATAAATGCA ACCTGTTGAC CCTGCAGTGC AGGAAAGACA TGGAGGGCCT TGTGGACACC
    AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG
    CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACGGA CATCTATCCC
    TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GTCAGCGGAT CCGTGAAGAA
    GTGCACTATT CAAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCATAC CAAAGTCCCA
    GCTCAAGCCC CTACGGAGGC TACACCCACC TACGAGACAG GCCAGCGGAA ACGCCTCATT
    TCCTCTGTGG ATGACTTCAC AGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765766.1
    CDS220..5472
    Translation

    Target ORF information:

    RefSeq Version XM_008767544.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tuberous sclerosis 2 (Tsc2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767544.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATAGGGC AGATCTGTGA TGTGGCAAAA ACTAAGAAAC TTGAAGAGCA TGCAGTGGAG 
    GCACTTTGGA AGGCTGTCTC AGACTTGCTA CAGCCAGAGC GGCCACCGGA GGCCCGACAT
    GCAGTTCTGG CCTTGTTGAA GGCCATTGTG CAGGGACAGG GTGATCGTTT GGGGGTTCTC
    CGAGCCCTCT TCTTCAAAGT GATCAAGGAC TATCCTTCCA ACGAAGACCT CCATGAAAGG
    CTAGAGGTTT TTAAGGCCCT CACAGACAAT GGAAGGCATA TTACCTACTT GGAAGAAGAA
    CTGGCAGAGT TTGTCCTGCA GTGGATGGAT GTTGGCTTGT CCTCAGAATT CCTTCTGGTA
    CTCGTGAACC TGGTCAAGTT CAACAGCTGC TACCTTGACG AATACATTGC GCCAATGGTT
    CACATGATTT GTCTGTTATG TATCCGGACA GTGTCCTCTG TGGACATCGA GGTGTCCTTG
    CAAGTGCTGG ATGCTGTGGT CTGCTACAAC TGCCTGCCTG CTGAGAGCCT GCCTCTGTTC
    ATTATCACCC TGTGCCGCAC CGTCAACGTC AAGGAGCTGT GTGAGCCGTG CTGGAAGCTG
    ATGCGAAACC TTCTAGGTAC CCATCTAGGC CACAGTGCCA TTTACAACAT GTGCCGTATC
    ATGGAGAACA GATCCTACAT GGAAGACGCC CCCTTGTTGA GGGGAGCCGT GTTCTTTGTT
    GGGATGGCGC TCTGGGGAGC TCACCGACTC TATTCGCTCA AGAACTCCCC CACATCTGTG
    CTGCCGTCTT TTTATGAGGC CATGACCTGT CCCAATGAGG TGGTGTCATA TGAGATTGTT
    CTGTCCATAA CAAGACTCAT CAAGAAGTAC AGGAAGGAGC TCCAGGCTGT GACATGGGAC
    ATTCTGCTGG ACATCATTGA ACGACTGCTC CAGCAACTCC AGAACCTGGA CAGCCCGGAA
    CTCAGGACCA TTGTCCATGA CCTGCTGACC ACTGTGGAGG AGCTGTGTGA CCAGAACGAG
    TTCCACGGAT CGCAAGAAAG ATACTATGAA CTGGTGGAGA GCTATGCAGA CCAGAGGCCT
    GAATCCTCTC TCTTAAACCT GATAACCTAC AGAGCCCAGT CTATCCACCC AGCCAAGGAT
    GGCTGGATCC AGAACCTGCA GTTGTTGATG GAGAGGTTCT TCAGGAACGA GTGCCGCAGT
    GCCGTGCGCA TCAAGGTGCT GGACGTGCTG TCCTTCGTGC TCCTCATCAA CAGGCAGTTC
    TACGAGGAGG AGCTGATTAA CTCGGTGGTC ATCTCACAGC TCTCCCACAT CCCCGAGGAT
    AAAGACCATC AGGTTCGAAA GCTGGCTACC CAGCTGCTGG TGGACCTGGC AGAGGGCTGC
    CACACCCACC ACTTCAACAG TCTGTTGGAC ATCATTGAAA AGGTGATGGC TCGCTCCCTT
    TCTCCACCCC TGGAGCTGGA GGAAAGGGAT CTGGCTGTGT ACTCGGCCTC CCTGGAGGAT
    GTGAAGACGG CAGTCCTCGG GCTCCTGGTC ATCCTTCAGA CCAAGCTGTA TACCTTGCCC
    GCCAGCCATG CCACACGAGT GTATGAGACA CTCATTAGCC ACATCCAGCT CCATTACAAG
    CACGGCTACT CCTTGCCCAT TGCTAGCAGC ATCCGGCTGC AGGCCTTTGA CTTCCTGCTG
    CTACTGCGGG CTGACTCGCT GCATCGACTG GGCCTGCCCA ACAAGGATGG GGTCGTGCGA
    TTCAGCCCTT ACTGCCTCTG TGACTGCGCG GAACTGGACA GGGCCTCAGA GAAGAAAGCC
    AGCGGACCCC TTTCACCGCC AACTGGGCCC CCCAGCCCTG TGCCAACGGG CCCTGCTGTG
    AGGCTCGGCC ACCTGCCCTA CTCCCTGCTT TTCCGTGTCC TGCTGCAGTG TTTGAAGCAG
    GAGACCGACT GGAAGGTGCT GAAGCTGGTG CTCAGCAAAC TCCCTGAGTC GCTGCGCTAC
    AAAGTCCTCA TTTTCACCTC CCCCTGCAGT GTTGACCAGC TGTCTTCTGC TCTCTGCTCC
    ATGCTTTCAG CCCCGAAGAC CCTTGAGCGG CTCCGAGGCA CCCCAGAAGG CTTCTCCAGA
    ACTGACCTGC ACTTGGCTGT GGTCCCTGTA CTGACAGCGT TAATATCTTA CCACAACTAT
    TTGGATAAGA CTAGACAGCG TGAGATGGTT TACTGCCTAG AACAAGGCCT TATCTACCGC
    TGTGCCAGCC AGTGTGTGGT AGCCTTGGCC ATCTGCAGTG TGGAGATGCC CGACATCATC
    ATCAAGGCGC TCCCTGTCCT GGTGGTGAAG CTGACACACA TCTCTGCTAC AGCTAGCATG
    GCCATCCCCC TCCTGGAGTT CCTGTCTACC TTGGCCAGGC TGCCTCACCT CTACAGGAAC
    TTCGCCGCAG AGCAGTATGC AAGTGTGTTT GCCATCTCCT TGCCCTACAC CAACCCCTCC
    AAGTTCAACC AGTACATTGT GTGTCTGGCC CATCACGTCA TAGCCATGTG GTTCATTAGG
    TGCCGACTGC CCTTTCGGAA GGATTTTGTC CCTTATATCA CTAAGGGTCT GCGTTCCAAT
    GTCCTCCTGT CCTTTGATGA TACCCCTGAG AAGGACAGTT TCAGAGCACG GAGCACCAGT
    CTTAATGAGA GACCCAAAAG TTTGAGGATA GCCAGAGCCC CCAAACAAGG CCTGAATAAC
    TCTCCACCTG TGAAAGAATT CAAAGAGAGC TGTGCAGCCG AGGCCTTCCG GTGCCGCAGC
    ATCAGTGTAT CTGAACATGT GGTCCGCAGC AGAATACAGA CATCTCTTAC CAGTGCCAGC
    TTGGGGTCTG CGGATGAGAA CTCTATGGCC CAGGCTGATG ACAACCTGAA GAATCTCCAC
    TTGGAACTCA CAGAAACATG TCTGGACATG ATGGCCCGAT ATGTGTTCTC CAACTTCACT
    GCGGTTCCCA AGAGGTCCCC TGTGGGAGAA TTCCTCCTGG CAGGGGGCAG GACCAAAACG
    TGGCTGGTTG GAAATAAGCT GGTCACTGTG ACAACGAGTG TGGGAACTGG TACGCGGTCG
    TTGCTGGGCC TGGACTCCGG GGATCTGCAG GGTGGCTCAG CATCAAGCTC TGATCCTGGC
    ACACATGTGA GGCAGACAAA GGAAGCACCG GCCAAACTGG AGTCCCAGGC TGGGCAGCAG
    GTGTCCCGTG GGGCCCGGGA CCGAGTCCGC TCCATGTCAG GGGGCCACGG CCTTCGAGTT
    GGTGTCCTGG ACACTTCAGC TCCCTACACC CCAGGAGGCC CTGCTTCTCT GGGAGCACAG
    GCAGCACCAG CAGCAAGGCC TGAGAAGCCC TGTGCAGGAG CCCAACTTCC GGCAGCGGAG
    AAGGCGAACC TGGCAGCCTA TGTGCCTTTG CTGACCCAGG GCTGGGCAGA GATCCTGGTC
    CGCAGGCCCA CAGGAAACAC CAGCTGGCTG ATGAGCTTGG AGAACCCGCT CAGCCCCTTT
    TCCTCAGACA TCAACAACAT GCCCCTGCAA GAGCTGTCTA ATGCCCTTAT GGCTGCTGAG
    CGCTTCAAGG AGCACCGGGA CACGGCCCTG TACAAGTCAT TGTCAGTGCC AGCGGCTGGC
    ACAGCCAAGC CTCCTACTCT CCCACGTTCT AACACAGTGG CTTCTTTCTC CTCCCTGTAC
    CAGCCCAGCT GCCAAGGACA GCTGCACAGG AGCGTTTCCT GGGCAGACTC GGCCGTGGTT
    CTTGAGGAGG GAAGTCCAGG AGAGGCTCAT GTGCCAGTGG AGCCCCCCGA ATTGGAGGAC
    TTTGAGGCAG CATTAGGTAC AGACAGGCAC TGCCAGCGGC CTGACGCCTA CAGCAGGTCT
    TCCTCAGCAT CTAGCCAGGA AGAAAAGTCC CACTTGGAGG AGCTGGCTGC AGGGGGTATC
    CCCATTGAGC GGGCCATCTC CTCTGAGGGG GCTCGGCCTA CAGTGGACCT GTCCTTCCAA
    CCCTCACAGC CTTTGAGCAA GTCTAGCTCT TCTCCTGAGT TACAGACCTT ACAGGACATC
    CTTGGAGACC TAGGGGACAA GACTGACATT GGACGGCTGA GTCCTGAGGC TAAGGTCCGA
    TCCCAGTCGG GGATCCTGGA TGGGGAAGCA GCTACCTGGT CAGCTCCGGG TGAAGAGAGC
    CGTATCACAG TCCCACCTGA AGGTCCCCTG CCTTCCAGTT CTCCCCGCTC CCCTAGTGGC
    CTCCGGCCCC GAGGCTATAC CATCTCTGAT TCAGCTCCAT CACGAAGGGG AAAGAGGGTA
    GAAAGGGACA ACTTCAAGAG CAGAACTGCG GCCTCCAGTG CTGAAAAGGT GCCAGGCATC
    AACCCTAGCT TTGTGTTCCT ACAGCTCTAC CATTCACCCT TTTTTGGTGA TGAGTCCAAT
    AAGCCCATCC TGTTGCCCAA CGAGTCCTTT GAACGGTCAG TGCAGCTCCT TGACCAGATC
    CCATCCTATG ACACTCACAA GATTGCTGTC CTGTATGTGG GAGAAGGCCA GAGCAGCAGT
    GAGCTGGCCA TCCTATCAAA TGAGCATGGC TCTTATAGGT ACACAGAGTT TCTGACAGGC
    CTGGGTCGGC TTATTGAGCT GAAGGACTGC CAACCAGACA AGGTGTACTT AGGTGGACTG
    GATGTGTGTG GCGAGGATGG ACAGTTCACG TACTGCTGGC ATGATGACAT CATGCAAGCT
    GTTTTCCACA TTGCTACCCT GATGCCCACC AAGGATGTGG ACAAGCACCG TTGTGACAAG
    AAACGGCACC TGGGCAATGA CTTTGTTTCT ATCATCTACA ATGATTCTGG TGAGGACTTC
    AAGCTGGGCA CCATTAAGGG CCAGTTCAAC TTCGTCCATG TGATCATCAC ACCGCTGGAC
    TATAAATGCA ACCTGTTGAC CCTGCAGTGC AGGAAAGACA TGGAGGGCCT TGTGGACACC
    AGTGTGGCCA AGATTGTGTC TGACCGCAAC CTGTCCTTTG TGGCCCGACA GATGGCCCTG
    CATGCAAATA TGGCCTCACA GGTACACCAC AGCCGATCCA ACCCCACGGA CATCTATCCC
    TCCAAGTGGA TCGCAAGACT CCGCCACATT AAGCGTCTCC GTCAGCGGAT CCGTGAAGAA
    GTGCACTATT CAAACCCAAG CTTGCCTCTG ATGCACCCTC CAGCCCATAC CAAAGTCCCA
    GCTCAAGCCC CTACGGAGGC TACACCCACC TACGAGACAG GCCAGCGGAA ACGCCTCATT
    TCCTCTGTGG ATGACTTCAC AGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.