tsc2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tsc2
Entrez Gene ID	100170546
Full Name	TSC complex subunit 2
General protein information	Preferred Names TSC complex subunit 2 Names tuberin tuberous sclerosis 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012970165.2	XP_012825619.1	tuberin isoform X1
XM_012970166.2	XP_012825620.1	tuberin isoform X2
XM_012970165.3	XP_012825619.2	tuberin isoform X1
XM_012970166.3	XP_012825620.2	tuberin isoform X2
NM_001130323.1	NP_001123795.1	tuberin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSC2	XP_523477.3
Mus musculus (house mouse)	Tsc2	NP_001034452.1
Rattus norvegicus (Norway rat)	Tsc2	NP_036812.2
Gallus gallus (chicken)	TSC2	XP_003642168.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011123	XP_309523.4
Canis lupus familiaris (dog)	TSC2	XP_852047.2
Bos taurus (cattle)	TSC2	XP_003587856.2
Drosophila melanogaster (fruit fly)	gig	NP_524177.1
Homo sapiens (human)	TSC2	NP_000539.2
	TSC2	XP_001083256.1
Danio rerio (zebrafish)	tsc2	XP_695912.6
Xenopus tropicalis (tropical clawed frog)	tsc2	NP_001123795.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tsc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tsc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa65915	XM_012970165.3 Latest version!	Xenopus tropicalis TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa65916	XM_012970166.3 Latest version!	Xenopus tropicalis TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa22998	NM_001130323.1 Latest version!	Xenopus tropicalis TSC complex subunit 2 (tsc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32398	XM_012970165.2 Latest version!	Xenopus tropicalis tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32399	XM_012970166.2 Latest version!	Xenopus tropicalis tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tsc2 ( XM_012970165.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825619.2 Xenopus tropicalis TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa65915
Clone ID Related Accession (Same CDS sequence)	XM_012970165.3
Accession Version	XM_012970165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGGGTG AAAGACTTGG CGTCCTTCGT 
GCCCATTTCT TCCGAGTCAT AAAAGAGTAT CCTATGAATG AGGACCTTGC AGAGCGACTG 
GAGGTTTTTA AAGCTCTCAC CGACAATGGG CGAGAAATTG CTTACTTAGA AGAAGAGCTC 
GCTGTCTTCG TCCTCCAGTG GATGGAAATT GGTTTGACTC CGGATTTCCT GCATGTTTTA 
GTTAACCTGG TTAAGTTCAA TAGCTGTTAC TTGGAACACT TTATTGCTCC TATGGTGCAG 
ATGATATGCC TCCTCTGTAA TCGCACGACA TCTCCTTACG ATATTGAGGT TTCCTTGAAG 
GTTCTGGATG CAGTGGTCTG CTATAACTGC CTTCCATCAG AGAGCATTCC TATATTTGTT 
ATGACCCTGT GCCGCACAAT AAATGTTAAG GAGCTGTGTG AACCATGCTG GAAGCTGATG 
CGAAACCTTC TGGGCACCCA TCTTGGGCAC AGCGCCATTT ACACTATGAG TCGTATAATG 
GAGGATAGAG CCCACATGGA AGATGCCCCT TTGCTAAGAG GAGCTGTGTT CTTTGTTGGG 
ATGGGTCTCT GGGGAGCTCA CAGACTCTTC TGTCTGAAGA ACTCCCCGAT ATCTGTTCTC 
CCATCTTTCC TGAAGGCTAT GAACTGTAAC AATGCCGTTG TCTCCTATGA GATTGTCCTC 
TCTGTCACTC GGCTAATCAA GAAGTACGGC AAGGATGTGC AGGCTGCCAC GTGGGACGTT 
CTCCTTGATA TCATCGAGCG ATTATTCCAG CAGCTTCAGG TGCTGCGGAG TCAGGAATTA 
AAGGCTGCGG TGCACAATCT CATGACCACC ACAGAGGAGC TTTATAACCA GAACGGATTC 
CATGGAGATG AAGAGAGATT TTTCGAGCTG GTTGAAAAAG GGGCAGATGA AAGACCTTTT 
GCATCAGTTG TGAACCTTAT CACCTACAGA GCTCAGTCCA TTCACCCTGG GAAGGAAGGG 
TGGATTATGA ATCTACAGAA ACTGATGGAT AGATATTTCA GGAGCGAGTC TCGCAGTGTT 
GTACGCATGA AAATCTTAGA TGTTCTGTCC TTTGCGTTGA GCATCAATCG CCAGTTTTAC 
GAGGATGAGT TGATCGAGAA GGTAGTTACT TGCCAGCTAG CCCATATACC CGAGGATAAG 
GACCACCAGG TGCGCAAACT CGCAACACAG CTCCTTGTGG ACCTGGCTGA ATGCTGTCAC 
TCCTGCCACT TCAATAGTCT CATGGATATC ATTGAGAAGG TGGTGAACCG TTCCTTGTCC 
ACACCAGAGT CAGGTCATGA TTCTTCTTCT TTGGAAGATG TGAAAACCTC TGTTCTGGGG 
CTGATGGTTA TTCTACAGAC CAAGCTATAC ATTCTGCCAG CCAGCCATGC CATGCGTGTG 
TACGAGATGC TGATTAATCA TATCCAGCTT CACTACAAAT ACAATTACAG CTCACCCATT 
GCTACCAGTA TACGCCTCCA GGTTTTTGAC TTCCTCCTAC ACCTCCGAGC TGACTCTTTG 
CGGCGTGTTG GGCTACCTAA CAAAGATGGC GCCTTCAGAT TCAGTCCCTA TTGTCTTTGT 
GAATCTGTAA ATGAACAAGA GAAGAGAGCA GCTGAACGCA AGCCAGCAGG CACCTTGTCA 
CCCCCCTCTG GTAGCCCCAG TGTCCCATCA CAGACTGTCA CTCCACGCAT GGGATTCTTG 
CCATTCTCAC TAGCATTTGG GGTTATCCTG CAGTGCCTAA AGCAGGAAAC TGACTGGAAA 
GTCCTGAAAC TAGTCCTCAA CAAGCTGCCA GAGTGTCTGC GTTACAAACT GCTCATTCTG 
TCATCTCCCT GCAATATAGA TCAACTGGCT GGCTCTCTCT GTGCTATGCT TACAGACAAG 
ACTGCAAGTG ATCGCCTTTA CATTACTCCC GATGGCTTCT CTCGTACTGA CCTTCAGCTG 
GCAGTGGTTC CTGTACTGAC AGCCTTAACC TCATACCACC AACACCTAGA GCTCTCTAAA 
CAGAGGGAAA TGGTAAGGTG TCTGGAAACA GGCCTGATCT TTCGCTGCGG CAAGCAGTGC 
GTTGTGGCCC TTTCCATGTG TGTGATCGAG ATGCCAAGCA TTCTGATAAA ATCCCTCCCG 
GTCCTTATCG TGAAGCTAAC CCATATCTCG GCCACTGTCA CCATGGCCAT ACCAATGCTG 
GAGTTTCTTT CAACTTTGGT CCGCCTGCCC CACTTATATG CAAACTTTGC CGCAGAGCAG 
TATGCCAGTG TGTTTGCCAT CTCCCTTCCC TACACCAACC CATCAAAGTT TAATCAATAC 
ATTGTATTCC TTGCACACCA TGTCATTGCC ATGTGGTTCA TCCGGTGCAG ACTATCCCTA 
CGGAGAGACT TTGTGCCATA TATAACAAAG GGTTTGCGAT CCAATGTCCT GCTGAGCTTT 
GATGACACAC CGGAGAAGGG CAGCTTTAGA GCTCGCAGCA CCAGCTTGAA CGAGAGACCC 
AAAAGCTTGA AGCTATCTAA ACACACAAAG CCTGGCTTCA GTGACACGTC CCCAGTTAAG 
GAGATGAGAG ATCTCTCCAA TGCCTTCCGC TCTCGCAGCA TCAGCGTGTC GGAGCATGCT 
GCGTACAGCC GTATGCAGAC TTCTGTCACC AGCTCCAGCT TGAGTTCAGC TGATGAGAGC 
TCTATGGCCC AGGCTGATGA CAGCCTGAAA AACCTTCACC TGGAATTGAC CGAAATCTGC 
CTGGATATGA TGGCACGATA CGCCTTTGCC AACTTCACAG CCGTGCCCAA AAGGTCGCCT 
GTAGGTGAGT TCCTGCTGGA TGGCGGAAGA ACGAAGACCT GGCTAGTTGG GAACAAGCTG 
GTTACCATTA CAACAAGTGT GGGCATGGGG ACACGTTCTC TTCTTGGCCT GGATACAGGA 
GGGGAGAACC ATGGTGACAC TGAGAACAGC TCCCCCGTGC AAACAGAGCA AGTCAAGGAG 
TTTCCGTCCA TGCTCGATCT TCAGCAAAGA AAGCCGGGTA TGCAAACGCC CCGTCACAGA 
GTCCGCTCCA TGTCTGGAGG GACTACACTG AGAGCTGGAT CACTAGAGGG GACTCCTCTT 
TCGGCCAGTA TGGGGCAGCC GGGGGCCACT CCAGTGACCA GAGCAGAGAA ACCTTCCCGT 
AGGACACAGA TGAAGAAAGA AAAGACCACC CTGGCTGACT TTGTTCCCGT GCTGACACAA 
GGCTGGGCAG AGGTATTGGT TCGCAGACCC ACAGGTAACA CCAGCTGGTT GATGAGCCTG 
GAAAATCCTC TGAGTCCCTT CTCTTCTGAC ATAAACAACA TGCCGCTGCA GGAGCTTTCT 
AATGCCCTAA TGGCAGCCGA GAGGCTCAAG GAGCATCGGG AGACAGCGCT CTACAAATCT 
CTCTCTGTGC CAGCCTCAAC TTTATCACCC TCAACAACCA AGTGTACCCT TCTACAGCGG 
GCAAACACAG TGGCCTCTGT GTCTCTCGTA ACCCAGTCCA GTGGTCAAGG AAAGCTGCTT 
AGGAGCATTT CCTGGGCAGA ATCTTGTGCT GTGCCAGAGG AAGGGGCGCA AGCTGATGGG 
GAAGCAGAGG AAGTGTCCAC TCCCGGGGGC TCTCAAGAGC TGGAAGATTT TGAGGCTGTG 
GTTAGCGAAG ACACCACTAG TGAAGAGAAG AGTGCTAAGA AGATGGAGTG CATTAGCAGG 
TCTTCATCCA CATCCAGTCA GGAAGAGTCT GCTGCTACCC AAACGGAGGA ATCCGAGGCT 
GGTGGAATAC CCATTGAGGG CTCCACTCGG GAGGTCCCAT TTCAGACTTT GAGCAAGTCC 
AGCTCATCTC CTGAACTTCA GACCTTGCAG GAGCTTCCTA AAGAGTTGGG CCCTACAGAA 
GATATAAAGA AGCCTTCTGC AGACTTCAAA ACACTACCCC ATGTAGAAAA CCCTTTAAGC 
CACAGAACTA AATCTGAAGA GGAACGCAGT GCTGGGGCAT TAGCCAAACC AGAAGGCGAA 
ATGCGACTTG AACCAGAGTT GCCTGTTCTG TCCCCACAAT CTCCAACTGG GCACCGCCCA 
AGGGGTCACA CTATCTCTGA TTCTGAGCCG TCCCGCAGAG GCAGGAGATT TCAGGTTGAT 
CCTTTTAAGA ACAGCACCAA ATCCACCAAA GCTGAGAAAG TGCCTGGAAT AGATCCAAGC 
TTTGTATTTT TGCAGCTGTA CCATTCCCCA TTCTTTGGTG ATGAGTCCAA TAAGCCTATA 
CTGGTCCCTA ACACACAGAC GTTTGAGAGA ACAATGGGCC TCCTAGATCG AATCCCGCCC 
TATGACACCC ACAAAATTGG TGTTCTTTAT GTTGGAGAGG GGCAGGTTGG CAACGAGCGC 
GCAATCCTAT CCAATGAGCA TGGCTCGTAC CGCTACACAC AGTTCCTCAC GGGGCTTGGG 
AAGCTCATCG AGCTGAAAGA CACTCAGCCA GACAAGATCT TCCTGGGAGG GTTGGACGGT 
TGTGGAGATG ACGGGCAGTT CACCTACTGC TGGCATGATG ATATTATGCA AGTCATTTTT 
CAGATCACCA CCCTGATGCC AAACAAAGAG ATAGATCCGA ACCGCTGCAA CAAGAAAAGG 
CACGTGGGCA ACAACTTTGT CAATATCATC TACAACGACT CGGGCGAGTT CTACAAGTTG 
GGCACCCTCA AGGGTCAGTT CAACTTTGTA GATGTCAACA TTCAGCCTTT GGACTGCGAG 
AGCAACCTGG TGACACTGCA ATGCCGAAAA GATATGGAAG GCCTTGTAGA TACCAGCGTG 
GCAAAAATTG TGTCTGACAA AAACCTACCC TTCCTGGCCC GCCAGATGGC TCTACATTCA 
AATATGGCTT CACAAGTCCA TCACAGCAGG TCCAACCCCA CAGATATTTA CCCTTCTAAA 
TGGATTGCCA GGCTGCGACA CATAAAGAAG ATGAGACCAA AGATACAAGA GGAGTTGCAG 
GAGCTGAGTG CCAACATGCC CTACCAGGTC CCTACTATGG CCAACAAGCC CCCTTCACAA 
AACCCACAGA CTCCCCCCGG CGCATTTGAA ACCGGCCAAA GGACAAGGCT CATTTCTGCT 
GTGGACGATT TCACGGAGTT TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825619.2
CDS	124..5550
Translation	MAKQMGKDPGLKEKVKMLLGLVPARVKTKPSEWKHPEYVITADLMKELSSESALHVRIRAIIQVCELAKTKKFEEHAVEAICQSVGDLIQPEQPVEARHHVLQLLKAIIVGQGERLGVLRAHFFRVIKEYPMNEDLAERLEVFKALTDNGREIAYLEEELAVFVLQWMEIGLTPDFLHVLVNLVKFNSCYLEHFIAPMVQMICLLCNRTTSPYDIEVSLKVLDAVVCYNCLPSESIPIFVMTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYTMSRIMEDRAHMEDAPLLRGAVFFVGMGLWGAHRLFCLKNSPISVLPSFLKAMNCNNAVVSYEIVLSVTRLIKKYGKDVQAATWDVLLDIIERLFQQLQVLRSQELKAAVHNLMTTTEELYNQNGFHGDEERFFELVEKGADERPFASVVNLITYRAQSIHPGKEGWIMNLQKLMDRYFRSESRSVVRMKILDVLSFALSINRQFYEDELIEKVVTCQLAHIPEDKDHQVRKLATQLLVDLAECCHSCHFNSLMDIIEKVVNRSLSTPESGHDSSSLEDVKTSVLGLMVILQTKLYILPASHAMRVYEMLINHIQLHYKYNYSSPIATSIRLQVFDFLLHLRADSLRRVGLPNKDGAFRFSPYCLCESVNEQEKRAAERKPAGTLSPPSGSPSVPSQTVTPRMGFLPFSLAFGVILQCLKQETDWKVLKLVLNKLPECLRYKLLILSSPCNIDQLAGSLCAMLTDKTASDRLYITPDGFSRTDLQLAVVPVLTALTSYHQHLELSKQREMVRCLETGLIFRCGKQCVVALSMCVIEMPSILIKSLPVLIVKLTHISATVTMAIPMLEFLSTLVRLPHLYANFAAEQYASVFAISLPYTNPSKFNQYIVFLAHHVIAMWFIRCRLSLRRDFVPYITKGLRSNVLLSFDDTPEKGSFRARSTSLNERPKSLKLSKHTKPGFSDTSPVKEMRDLSNAFRSRSISVSEHAAYSRMQTSVTSSSLSSADESSMAQADDSLKNLHLELTEICLDMMARYAFANFTAVPKRSPVGEFLLDGGRTKTWLVGNKLVTITTSVGMGTRSLLGLDTGGENHGDTENSSPVQTEQVKEFPSMLDLQQRKPGMQTPRHRVRSMSGGTTLRAGSLEGTPLSASMGQPGATPVTRAEKPSRRTQMKKEKTTLADFVPVLTQGWAEVLVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERLKEHRETALYKSLSVPASTLSPSTTKCTLLQRANTVASVSLVTQSSGQGKLLRSISWAESCAVPEEGAQADGEAEEVSTPGGSQELEDFEAVVSEDTTSEEKSAKKMECISRSSSTSSQEESAATQTEESEAGGIPIEGSTREVPFQTLSKSSSSPELQTLQELPKELGPTEDIKKPSADFKTLPHVENPLSHRTKSEEERSAGALAKPEGEMRLEPELPVLSPQSPTGHRPRGHTISDSEPSRRGRRFQVDPFKNSTKSTKAEKVPGIDPSFVFLQLYHSPFFGDESNKPILVPNTQTFERTMGLLDRIPPYDTHKIGVLYVGEGQVGNERAILSNEHGSYRYTQFLTGLGKLIELKDTQPDKIFLGGLDGCGDDGQFTYCWHDDIMQVIFQITTLMPNKEIDPNRCNKKRHVGNNFVNIIYNDSGEFYKLGTLKGQFNFVDVNIQPLDCESNLVTLQCRKDMEGLVDTSVAKIVSDKNLPFLARQMALHSNMASQVHHSRSNPTDIYPSKWIARLRHIKKMRPKIQEELQELSANMPYQVPTMANKPPSQNPQTPPGAFETGQRTRLISAVDDFTEFA

Target ORF information:

RefSeq Version	XM_012970165.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TSC complex subunit 2 (tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970165.3

ORF Insert Sequence:

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGGGTG AAAGACTTGG CGTCCTTCGT 
GCCCATTTCT TCCGAGTCAT AAAAGAGTAT CCTATGAATG AGGACCTTGC AGAGCGACTG 
GAGGTTTTTA AAGCTCTCAC CGACAATGGG CGAGAAATTG CTTACTTAGA AGAAGAGCTC 
GCTGTCTTCG TCCTCCAGTG GATGGAAATT GGTTTGACTC CGGATTTCCT GCATGTTTTA 
GTTAACCTGG TTAAGTTCAA TAGCTGTTAC TTGGAACACT TTATTGCTCC TATGGTGCAG 
ATGATATGCC TCCTCTGTAA TCGCACGACA TCTCCTTACG ATATTGAGGT TTCCTTGAAG 
GTTCTGGATG CAGTGGTCTG CTATAACTGC CTTCCATCAG AGAGCATTCC TATATTTGTT 
ATGACCCTGT GCCGCACAAT AAATGTTAAG GAGCTGTGTG AACCATGCTG GAAGCTGATG 
CGAAACCTTC TGGGCACCCA TCTTGGGCAC AGCGCCATTT ACACTATGAG TCGTATAATG 
GAGGATAGAG CCCACATGGA AGATGCCCCT TTGCTAAGAG GAGCTGTGTT CTTTGTTGGG 
ATGGGTCTCT GGGGAGCTCA CAGACTCTTC TGTCTGAAGA ACTCCCCGAT ATCTGTTCTC 
CCATCTTTCC TGAAGGCTAT GAACTGTAAC AATGCCGTTG TCTCCTATGA GATTGTCCTC 
TCTGTCACTC GGCTAATCAA GAAGTACGGC AAGGATGTGC AGGCTGCCAC GTGGGACGTT 
CTCCTTGATA TCATCGAGCG ATTATTCCAG CAGCTTCAGG TGCTGCGGAG TCAGGAATTA 
AAGGCTGCGG TGCACAATCT CATGACCACC ACAGAGGAGC TTTATAACCA GAACGGATTC 
CATGGAGATG AAGAGAGATT TTTCGAGCTG GTTGAAAAAG GGGCAGATGA AAGACCTTTT 
GCATCAGTTG TGAACCTTAT CACCTACAGA GCTCAGTCCA TTCACCCTGG GAAGGAAGGG 
TGGATTATGA ATCTACAGAA ACTGATGGAT AGATATTTCA GGAGCGAGTC TCGCAGTGTT 
GTACGCATGA AAATCTTAGA TGTTCTGTCC TTTGCGTTGA GCATCAATCG CCAGTTTTAC 
GAGGATGAGT TGATCGAGAA GGTAGTTACT TGCCAGCTAG CCCATATACC CGAGGATAAG 
GACCACCAGG TGCGCAAACT CGCAACACAG CTCCTTGTGG ACCTGGCTGA ATGCTGTCAC 
TCCTGCCACT TCAATAGTCT CATGGATATC ATTGAGAAGG TGGTGAACCG TTCCTTGTCC 
ACACCAGAGT CAGGTCATGA TTCTTCTTCT TTGGAAGATG TGAAAACCTC TGTTCTGGGG 
CTGATGGTTA TTCTACAGAC CAAGCTATAC ATTCTGCCAG CCAGCCATGC CATGCGTGTG 
TACGAGATGC TGATTAATCA TATCCAGCTT CACTACAAAT ACAATTACAG CTCACCCATT 
GCTACCAGTA TACGCCTCCA GGTTTTTGAC TTCCTCCTAC ACCTCCGAGC TGACTCTTTG 
CGGCGTGTTG GGCTACCTAA CAAAGATGGC GCCTTCAGAT TCAGTCCCTA TTGTCTTTGT 
GAATCTGTAA ATGAACAAGA GAAGAGAGCA GCTGAACGCA AGCCAGCAGG CACCTTGTCA 
CCCCCCTCTG GTAGCCCCAG TGTCCCATCA CAGACTGTCA CTCCACGCAT GGGATTCTTG 
CCATTCTCAC TAGCATTTGG GGTTATCCTG CAGTGCCTAA AGCAGGAAAC TGACTGGAAA 
GTCCTGAAAC TAGTCCTCAA CAAGCTGCCA GAGTGTCTGC GTTACAAACT GCTCATTCTG 
TCATCTCCCT GCAATATAGA TCAACTGGCT GGCTCTCTCT GTGCTATGCT TACAGACAAG 
ACTGCAAGTG ATCGCCTTTA CATTACTCCC GATGGCTTCT CTCGTACTGA CCTTCAGCTG 
GCAGTGGTTC CTGTACTGAC AGCCTTAACC TCATACCACC AACACCTAGA GCTCTCTAAA 
CAGAGGGAAA TGGTAAGGTG TCTGGAAACA GGCCTGATCT TTCGCTGCGG CAAGCAGTGC 
GTTGTGGCCC TTTCCATGTG TGTGATCGAG ATGCCAAGCA TTCTGATAAA ATCCCTCCCG 
GTCCTTATCG TGAAGCTAAC CCATATCTCG GCCACTGTCA CCATGGCCAT ACCAATGCTG 
GAGTTTCTTT CAACTTTGGT CCGCCTGCCC CACTTATATG CAAACTTTGC CGCAGAGCAG 
TATGCCAGTG TGTTTGCCAT CTCCCTTCCC TACACCAACC CATCAAAGTT TAATCAATAC 
ATTGTATTCC TTGCACACCA TGTCATTGCC ATGTGGTTCA TCCGGTGCAG ACTATCCCTA 
CGGAGAGACT TTGTGCCATA TATAACAAAG GGTTTGCGAT CCAATGTCCT GCTGAGCTTT 
GATGACACAC CGGAGAAGGG CAGCTTTAGA GCTCGCAGCA CCAGCTTGAA CGAGAGACCC 
AAAAGCTTGA AGCTATCTAA ACACACAAAG CCTGGCTTCA GTGACACGTC CCCAGTTAAG 
GAGATGAGAG ATCTCTCCAA TGCCTTCCGC TCTCGCAGCA TCAGCGTGTC GGAGCATGCT 
GCGTACAGCC GTATGCAGAC TTCTGTCACC AGCTCCAGCT TGAGTTCAGC TGATGAGAGC 
TCTATGGCCC AGGCTGATGA CAGCCTGAAA AACCTTCACC TGGAATTGAC CGAAATCTGC 
CTGGATATGA TGGCACGATA CGCCTTTGCC AACTTCACAG CCGTGCCCAA AAGGTCGCCT 
GTAGGTGAGT TCCTGCTGGA TGGCGGAAGA ACGAAGACCT GGCTAGTTGG GAACAAGCTG 
GTTACCATTA CAACAAGTGT GGGCATGGGG ACACGTTCTC TTCTTGGCCT GGATACAGGA 
GGGGAGAACC ATGGTGACAC TGAGAACAGC TCCCCCGTGC AAACAGAGCA AGTCAAGGAG 
TTTCCGTCCA TGCTCGATCT TCAGCAAAGA AAGCCGGGTA TGCAAACGCC CCGTCACAGA 
GTCCGCTCCA TGTCTGGAGG GACTACACTG AGAGCTGGAT CACTAGAGGG GACTCCTCTT 
TCGGCCAGTA TGGGGCAGCC GGGGGCCACT CCAGTGACCA GAGCAGAGAA ACCTTCCCGT 
AGGACACAGA TGAAGAAAGA AAAGACCACC CTGGCTGACT TTGTTCCCGT GCTGACACAA 
GGCTGGGCAG AGGTATTGGT TCGCAGACCC ACAGGTAACA CCAGCTGGTT GATGAGCCTG 
GAAAATCCTC TGAGTCCCTT CTCTTCTGAC ATAAACAACA TGCCGCTGCA GGAGCTTTCT 
AATGCCCTAA TGGCAGCCGA GAGGCTCAAG GAGCATCGGG AGACAGCGCT CTACAAATCT 
CTCTCTGTGC CAGCCTCAAC TTTATCACCC TCAACAACCA AGTGTACCCT TCTACAGCGG 
GCAAACACAG TGGCCTCTGT GTCTCTCGTA ACCCAGTCCA GTGGTCAAGG AAAGCTGCTT 
AGGAGCATTT CCTGGGCAGA ATCTTGTGCT GTGCCAGAGG AAGGGGCGCA AGCTGATGGG 
GAAGCAGAGG AAGTGTCCAC TCCCGGGGGC TCTCAAGAGC TGGAAGATTT TGAGGCTGTG 
GTTAGCGAAG ACACCACTAG TGAAGAGAAG AGTGCTAAGA AGATGGAGTG CATTAGCAGG 
TCTTCATCCA CATCCAGTCA GGAAGAGTCT GCTGCTACCC AAACGGAGGA ATCCGAGGCT 
GGTGGAATAC CCATTGAGGG CTCCACTCGG GAGGTCCCAT TTCAGACTTT GAGCAAGTCC 
AGCTCATCTC CTGAACTTCA GACCTTGCAG GAGCTTCCTA AAGAGTTGGG CCCTACAGAA 
GATATAAAGA AGCCTTCTGC AGACTTCAAA ACACTACCCC ATGTAGAAAA CCCTTTAAGC 
CACAGAACTA AATCTGAAGA GGAACGCAGT GCTGGGGCAT TAGCCAAACC AGAAGGCGAA 
ATGCGACTTG AACCAGAGTT GCCTGTTCTG TCCCCACAAT CTCCAACTGG GCACCGCCCA 
AGGGGTCACA CTATCTCTGA TTCTGAGCCG TCCCGCAGAG GCAGGAGATT TCAGGTTGAT 
CCTTTTAAGA ACAGCACCAA ATCCACCAAA GCTGAGAAAG TGCCTGGAAT AGATCCAAGC 
TTTGTATTTT TGCAGCTGTA CCATTCCCCA TTCTTTGGTG ATGAGTCCAA TAAGCCTATA 
CTGGTCCCTA ACACACAGAC GTTTGAGAGA ACAATGGGCC TCCTAGATCG AATCCCGCCC 
TATGACACCC ACAAAATTGG TGTTCTTTAT GTTGGAGAGG GGCAGGTTGG CAACGAGCGC 
GCAATCCTAT CCAATGAGCA TGGCTCGTAC CGCTACACAC AGTTCCTCAC GGGGCTTGGG 
AAGCTCATCG AGCTGAAAGA CACTCAGCCA GACAAGATCT TCCTGGGAGG GTTGGACGGT 
TGTGGAGATG ACGGGCAGTT CACCTACTGC TGGCATGATG ATATTATGCA AGTCATTTTT 
CAGATCACCA CCCTGATGCC AAACAAAGAG ATAGATCCGA ACCGCTGCAA CAAGAAAAGG 
CACGTGGGCA ACAACTTTGT CAATATCATC TACAACGACT CGGGCGAGTT CTACAAGTTG 
GGCACCCTCA AGGGTCAGTT CAACTTTGTA GATGTCAACA TTCAGCCTTT GGACTGCGAG 
AGCAACCTGG TGACACTGCA ATGCCGAAAA GATATGGAAG GCCTTGTAGA TACCAGCGTG 
GCAAAAATTG TGTCTGACAA AAACCTACCC TTCCTGGCCC GCCAGATGGC TCTACATTCA 
AATATGGCTT CACAAGTCCA TCACAGCAGG TCCAACCCCA CAGATATTTA CCCTTCTAAA 
TGGATTGCCA GGCTGCGACA CATAAAGAAG ATGAGACCAA AGATACAAGA GGAGTTGCAG 
GAGCTGAGTG CCAACATGCC CTACCAGGTC CCTACTATGG CCAACAAGCC CCCTTCACAA 
AACCCACAGA CTCCCCCCGG CGCATTTGAA ACCGGCCAAA GGACAAGGCT CATTTCTGCT 
GTGGACGATT TCACGGAGTT TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_012970166.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825620.2 Xenopus tropicalis TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.

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CloneID	OXa65916
Clone ID Related Accession (Same CDS sequence)	XM_012970166.3
Accession Version	XM_012970166.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970166.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGGGTG AAAGACTTGG CGTCCTTCGT 
GCCCATTTCT TCCGAGTCAT AAAAGAGTAT CCTATGAATG AGGACCTTGC AGAGCGACTG 
GAGGTTTTTA AAGCTCTCAC CGACAATGGG CGAGAAATTG CTTACTTAGA AGAAGAGCTC 
GCTGTCTTCG TCCTCCAGTG GATGGAAATT GGTTTGACTC CGGATTTCCT GCATGTTTTA 
GTTAACCTGG TTAAGTTCAA TAGCTGTTAC TTGGAACACT TTATTGCTCC TATGGTGCAG 
ATGATATGCC TCCTCTGTAA TCGCACGACA TCTCCTTACG ATATTGAGGT TTCCTTGAAG 
GTTCTGGATG CAGTGGTCTG CTATAACTGC CTTCCATCAG AGAGCATTCC TATATTTGTT 
ATGACCCTGT GCCGCACAAT AAATGTTAAG GAGCTGTGTG AACCATGCTG GAAGCTGATG 
CGAAACCTTC TGGGCACCCA TCTTGGGCAC AGCGCCATTT ACACTATGAG TCGTATAATG 
GAGGATAGAG CCCACATGGA AGATGCCCCT TTGCTAAGAG GAGCTGTGTT CTTTGTTGGG 
ATGGGTCTCT GGGGAGCTCA CAGACTCTTC TGTCTGAAGA ACTCCCCGAT ATCTGTTCTC 
CCATCTTTCC TGAAGGCTAT GAACTGTAAC AATGCCGTTG TCTCCTATGA GATTGTCCTC 
TCTGTCACTC GGCTAATCAA GAAGTACGGC AAGGATGTGC AGGCTGCCAC GTGGGACGTT 
CTCCTTGATA TCATCGAGCG ATTATTCCAG CAGCTTCAGG TGCTGCGGAG TCAGGAATTA 
AAGGCTGCGG TGCACAATCT CATGACCACC ACAGAGGAGC TTTATAACCA GAACGGATTC 
CATGGAGATG AAGAGAGATT TTTCGAGCTG GTTGAAAAAG GGGCAGATGA AAGACCTTTT 
GCATCAGTTG TGAACCTTAT CACCTACAGA GCTCAGTCCA TTCACCCTGG GAAGGAAGGG 
TGGATTATGA ATCTACAGAA ACTGATGGAT AGATATTTCA GGAGCGAGTC TCGCAGTGTT 
GTACGCATGA AAATCTTAGA TGTTCTGTCC TTTGCGTTGA GCATCAATCG CCAGTTTTAC 
GAGGATGAGT TGATCGAGAA GGTAGTTACT TGCCAGCTAG CCCATATACC CGAGGATAAG 
GACCACCAGG TGCGCAAACT CGCAACACAG CTCCTTGTGG ACCTGGCTGA ATGCTGTCAC 
TCCTGCCACT TCAATAGTCT CATGGATATC ATTGAGAAGG TGGTGAACCG TTCCTTGTCC 
ACACCAGAGT CAGGTCATGA TTCTTCTTCT TTGGAAGATG TGAAAACCTC TGTTCTGGGG 
CTGATGGTTA TTCTACAGAC CAAGCTATAC ATTCTGCCAG CCAGCCATGC CATGCGTGTG 
TACGAGATGC TGATTAATCA TATCCAGCTT CACTACAAAT ACAATTACAG CTCACCCATT 
GCTACCAGTA TACGCCTCCA GGTTTTTGAC TTCCTCCTAC ACCTCCGAGC TGACTCTTTG 
CGGCGTGTTG GGCTACCTAA CAAAGATGGC GCCTTCAGAT TCAGTCCCTA TTGTCTTTGT 
GAATCTGTAA ATGAACAAGA GAAGAGAGCA GCTGAACGCA AGCCAGCAGG CACCTTGTCA 
CCCCCCTCTG GTAGCCCCAG TGTCCCATCA CAGACTGTCA CTCCACGCAT GGGATTCTTG 
CCATTCTCAC TAGCATTTGG GGTTATCCTG CAGTGCCTAA AGCAGGAAAC TGACTGGAAA 
GTCCTGAAAC TAGTCCTCAA CAAGCTGCCA GAGTGTCTGC GTTACAAACT GCTCATTCTG 
TCATCTCCCT GCAATATAGA TCAACTGGCT GGCTCTCTCT GTGCTATGCT TACAGACAAG 
ACTGCAAGTG ATCGCCTTTA CATTACTCCC GATGGCTTCT CTCGTACTGA CCTTCAGCTG 
GCAGTGGTTC CTGTACTGAC AGCCTTAACC TCATACCACC AACACCTAGA GCTCTCTAAA 
CAGAGGGAAA TGGTAAGGTG TCTGGAAACA GGCCTGATCT TTCGCTGCGG CAAGCAGTGC 
GTTGTGGCCC TTTCCATGTG TGTGATCGAG ATGCCAAGCA TTCTGATAAA ATCCCTCCCG 
GTCCTTATCG TGAAGCTAAC CCATATCTCG GCCACTGTCA CCATGGCCAT ACCAATGCTG 
GAGTTTCTTT CAACTTTGGT CCGCCTGCCC CACTTATATG CAAACTTTGC CGCAGAGCAG 
TATGCCAGTG TGTTTGCCAT CTCCCTTCCC TACACCAACC CATCAAAGTT TAATCAATAC 
ATTGTATTCC TTGCACACCA TGTCATTGCC ATGTGGTTCA TCCGGTGCAG ACTATCCCTA 
CGGAGAGACT TTGTGCCATA TATAACAAAG GGTTTGCGAT CCAATGTCCT GCTGAGCTTT 
GATGACACAC CGGAGAAGGG CAGCTTTAGA GCTCGCAGCA CCAGCTTGAA CGAGAGACCC 
AAAAGCCGTA TGCAGACTTC TGTCACCAGC TCCAGCTTGA GTTCAGCTGA TGAGAGCTCT 
ATGGCCCAGG CTGATGACAG CCTGAAAAAC CTTCACCTGG AATTGACCGA AATCTGCCTG 
GATATGATGG CACGATACGC CTTTGCCAAC TTCACAGCCG TGCCCAAAAG GTCGCCTGTA 
GGTGAGTTCC TGCTGGATGG CGGAAGAACG AAGACCTGGC TAGTTGGGAA CAAGCTGGTT 
ACCATTACAA CAAGTGTGGG CATGGGGACA CGTTCTCTTC TTGGCCTGGA TACAGGAGGG 
GAGAACCATG GTGACACTGA GAACAGCTCC CCCGTGCAAA CAGAGCAAGT CAAGGAGTTT 
CCGTCCATGC TCGATCTTCA GCAAAGAAAG CCGGGTATGC AAACGCCCCG TCACAGAGTC 
CGCTCCATGT CTGGAGGGAC TACACTGAGA GCTGGATCAC TAGAGGGGAC TCCTCTTTCG 
GCCAGTATGG GGCAGCCGGG GGCCACTCCA GTGACCAGAG CAGAGAAACC TTCCCGTAGG 
ACACAGATGA AGAAAGAAAA GACCACCCTG GCTGACTTTG TTCCCGTGCT GACACAAGGC 
TGGGCAGAGG TATTGGTTCG CAGACCCACA GGTAACACCA GCTGGTTGAT GAGCCTGGAA 
AATCCTCTGA GTCCCTTCTC TTCTGACATA AACAACATGC CGCTGCAGGA GCTTTCTAAT 
GCCCTAATGG CAGCCGAGAG GCTCAAGGAG CATCGGGAGA CAGCGCTCTA CAAATCTCTC 
TCTGTGCCAG CCTCAACTTT ATCACCCTCA ACAACCAAGT GTACCCTTCT ACAGCGGGCA 
AACACAGTGG CCTCTGTGTC TCTCGTAACC CAGTCCAGTG GTCAAGGAAA GCTGCTTAGG 
AGCATTTCCT GGGCAGAATC TTGTGCTGTG CCAGAGGAAG GGGCGCAAGC TGATGGGGAA 
GCAGAGGAAG TGTCCACTCC CGGGGGCTCT CAAGAGCTGG AAGATTTTGA GGCTGTGGTT 
AGCGAAGACA CCACTAGTGA AGAGAAGAGT GCTAAGAAGA TGGAGTGCAT TAGCAGGTCT 
TCATCCACAT CCAGTCAGGA AGAGTCTGCT GCTACCCAAA CGGAGGAATC CGAGGCTGGT 
GGAATACCCA TTGAGGGCTC CACTCGGGAG GTCCCATTTC AGACTTTGAG CAAGTCCAGC 
TCATCTCCTG AACTTCAGAC CTTGCAGGAG CTTCCTAAAG AGTTGGGCCC TACAGAAGAT 
ATAAAGAAGC CTTCTGCAGA CTTCAAAACA CTACCCCATG TAGAAAACCC TTTAAGCCAC 
AGAACTAAAT CTGAAGAGGA ACGCAGTGCT GGGGCATTAG CCAAACCAGA AGGCGAAATG 
CGACTTGAAC CAGAGTTGCC TGTTCTGTCC CCACAATCTC CAACTGGGCA CCGCCCAAGG 
GGTCACACTA TCTCTGATTC TGAGCCGTCC CGCAGAGGCA GGAGATTTCA GGTTGATCCT 
TTTAAGAACA GCACCAAATC CACCAAAGCT GAGAAAGTGC CTGGAATAGA TCCAAGCTTT 
GTATTTTTGC AGCTGTACCA TTCCCCATTC TTTGGTGATG AGTCCAATAA GCCTATACTG 
GTCCCTAACA CACAGACGTT TGAGAGAACA ATGGGCCTCC TAGATCGAAT CCCGCCCTAT 
GACACCCACA AAATTGGTGT TCTTTATGTT GGAGAGGGGC AGGTTGGCAA CGAGCGCGCA 
ATCCTATCCA ATGAGCATGG CTCGTACCGC TACACACAGT TCCTCACGGG GCTTGGGAAG 
CTCATCGAGC TGAAAGACAC TCAGCCAGAC AAGATCTTCC TGGGAGGGTT GGACGGTTGT 
GGAGATGACG GGCAGTTCAC CTACTGCTGG CATGATGATA TTATGCAAGT CATTTTTCAG 
ATCACCACCC TGATGCCAAA CAAAGAGATA GATCCGAACC GCTGCAACAA GAAAAGGCAC 
GTGGGCAACA ACTTTGTCAA TATCATCTAC AACGACTCGG GCGAGTTCTA CAAGTTGGGC 
ACCCTCAAGG GTCAGTTCAA CTTTGTAGAT GTCAACATTC AGCCTTTGGA CTGCGAGAGC 
AACCTGGTGA CACTGCAATG CCGAAAAGAT ATGGAAGGCC TTGTAGATAC CAGCGTGGCA 
AAAATTGTGT CTGACAAAAA CCTACCCTTC CTGGCCCGCC AGATGGCTCT ACATTCAAAT 
ATGGCTTCAC AAGTCCATCA CAGCAGGTCC AACCCCACAG ATATTTACCC TTCTAAATGG 
ATTGCCAGGC TGCGACACAT AAAGAAGATG AGACCAAAGA TACAAGAGGA GTTGCAGGAG 
CTGAGTGCCA ACATGCCCTA CCAGGTCCCT ACTATGGCCA ACAAGCCCCC TTCACAAAAC 
CCACAGACTC CCCCCGGCGC ATTTGAAACC GGCCAAAGGA CAAGGCTCAT TTCTGCTGTG 
GACGATTTCA CGGAGTTTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825620.2
CDS	124..5427
Translation	MAKQMGKDPGLKEKVKMLLGLVPARVKTKPSEWKHPEYVITADLMKELSSESALHVRIRAIIQVCELAKTKKFEEHAVEAICQSVGDLIQPEQPVEARHHVLQLLKAIIVGQGERLGVLRAHFFRVIKEYPMNEDLAERLEVFKALTDNGREIAYLEEELAVFVLQWMEIGLTPDFLHVLVNLVKFNSCYLEHFIAPMVQMICLLCNRTTSPYDIEVSLKVLDAVVCYNCLPSESIPIFVMTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYTMSRIMEDRAHMEDAPLLRGAVFFVGMGLWGAHRLFCLKNSPISVLPSFLKAMNCNNAVVSYEIVLSVTRLIKKYGKDVQAATWDVLLDIIERLFQQLQVLRSQELKAAVHNLMTTTEELYNQNGFHGDEERFFELVEKGADERPFASVVNLITYRAQSIHPGKEGWIMNLQKLMDRYFRSESRSVVRMKILDVLSFALSINRQFYEDELIEKVVTCQLAHIPEDKDHQVRKLATQLLVDLAECCHSCHFNSLMDIIEKVVNRSLSTPESGHDSSSLEDVKTSVLGLMVILQTKLYILPASHAMRVYEMLINHIQLHYKYNYSSPIATSIRLQVFDFLLHLRADSLRRVGLPNKDGAFRFSPYCLCESVNEQEKRAAERKPAGTLSPPSGSPSVPSQTVTPRMGFLPFSLAFGVILQCLKQETDWKVLKLVLNKLPECLRYKLLILSSPCNIDQLAGSLCAMLTDKTASDRLYITPDGFSRTDLQLAVVPVLTALTSYHQHLELSKQREMVRCLETGLIFRCGKQCVVALSMCVIEMPSILIKSLPVLIVKLTHISATVTMAIPMLEFLSTLVRLPHLYANFAAEQYASVFAISLPYTNPSKFNQYIVFLAHHVIAMWFIRCRLSLRRDFVPYITKGLRSNVLLSFDDTPEKGSFRARSTSLNERPKSRMQTSVTSSSLSSADESSMAQADDSLKNLHLELTEICLDMMARYAFANFTAVPKRSPVGEFLLDGGRTKTWLVGNKLVTITTSVGMGTRSLLGLDTGGENHGDTENSSPVQTEQVKEFPSMLDLQQRKPGMQTPRHRVRSMSGGTTLRAGSLEGTPLSASMGQPGATPVTRAEKPSRRTQMKKEKTTLADFVPVLTQGWAEVLVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERLKEHRETALYKSLSVPASTLSPSTTKCTLLQRANTVASVSLVTQSSGQGKLLRSISWAESCAVPEEGAQADGEAEEVSTPGGSQELEDFEAVVSEDTTSEEKSAKKMECISRSSSTSSQEESAATQTEESEAGGIPIEGSTREVPFQTLSKSSSSPELQTLQELPKELGPTEDIKKPSADFKTLPHVENPLSHRTKSEEERSAGALAKPEGEMRLEPELPVLSPQSPTGHRPRGHTISDSEPSRRGRRFQVDPFKNSTKSTKAEKVPGIDPSFVFLQLYHSPFFGDESNKPILVPNTQTFERTMGLLDRIPPYDTHKIGVLYVGEGQVGNERAILSNEHGSYRYTQFLTGLGKLIELKDTQPDKIFLGGLDGCGDDGQFTYCWHDDIMQVIFQITTLMPNKEIDPNRCNKKRHVGNNFVNIIYNDSGEFYKLGTLKGQFNFVDVNIQPLDCESNLVTLQCRKDMEGLVDTSVAKIVSDKNLPFLARQMALHSNMASQVHHSRSNPTDIYPSKWIARLRHIKKMRPKIQEELQELSANMPYQVPTMANKPPSQNPQTPPGAFETGQRTRLISAVDDFTEFA

Target ORF information:

RefSeq Version	XM_012970166.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TSC complex subunit 2 (tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970166.3

ORF Insert Sequence:

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGGGTG AAAGACTTGG CGTCCTTCGT 
GCCCATTTCT TCCGAGTCAT AAAAGAGTAT CCTATGAATG AGGACCTTGC AGAGCGACTG 
GAGGTTTTTA AAGCTCTCAC CGACAATGGG CGAGAAATTG CTTACTTAGA AGAAGAGCTC 
GCTGTCTTCG TCCTCCAGTG GATGGAAATT GGTTTGACTC CGGATTTCCT GCATGTTTTA 
GTTAACCTGG TTAAGTTCAA TAGCTGTTAC TTGGAACACT TTATTGCTCC TATGGTGCAG 
ATGATATGCC TCCTCTGTAA TCGCACGACA TCTCCTTACG ATATTGAGGT TTCCTTGAAG 
GTTCTGGATG CAGTGGTCTG CTATAACTGC CTTCCATCAG AGAGCATTCC TATATTTGTT 
ATGACCCTGT GCCGCACAAT AAATGTTAAG GAGCTGTGTG AACCATGCTG GAAGCTGATG 
CGAAACCTTC TGGGCACCCA TCTTGGGCAC AGCGCCATTT ACACTATGAG TCGTATAATG 
GAGGATAGAG CCCACATGGA AGATGCCCCT TTGCTAAGAG GAGCTGTGTT CTTTGTTGGG 
ATGGGTCTCT GGGGAGCTCA CAGACTCTTC TGTCTGAAGA ACTCCCCGAT ATCTGTTCTC 
CCATCTTTCC TGAAGGCTAT GAACTGTAAC AATGCCGTTG TCTCCTATGA GATTGTCCTC 
TCTGTCACTC GGCTAATCAA GAAGTACGGC AAGGATGTGC AGGCTGCCAC GTGGGACGTT 
CTCCTTGATA TCATCGAGCG ATTATTCCAG CAGCTTCAGG TGCTGCGGAG TCAGGAATTA 
AAGGCTGCGG TGCACAATCT CATGACCACC ACAGAGGAGC TTTATAACCA GAACGGATTC 
CATGGAGATG AAGAGAGATT TTTCGAGCTG GTTGAAAAAG GGGCAGATGA AAGACCTTTT 
GCATCAGTTG TGAACCTTAT CACCTACAGA GCTCAGTCCA TTCACCCTGG GAAGGAAGGG 
TGGATTATGA ATCTACAGAA ACTGATGGAT AGATATTTCA GGAGCGAGTC TCGCAGTGTT 
GTACGCATGA AAATCTTAGA TGTTCTGTCC TTTGCGTTGA GCATCAATCG CCAGTTTTAC 
GAGGATGAGT TGATCGAGAA GGTAGTTACT TGCCAGCTAG CCCATATACC CGAGGATAAG 
GACCACCAGG TGCGCAAACT CGCAACACAG CTCCTTGTGG ACCTGGCTGA ATGCTGTCAC 
TCCTGCCACT TCAATAGTCT CATGGATATC ATTGAGAAGG TGGTGAACCG TTCCTTGTCC 
ACACCAGAGT CAGGTCATGA TTCTTCTTCT TTGGAAGATG TGAAAACCTC TGTTCTGGGG 
CTGATGGTTA TTCTACAGAC CAAGCTATAC ATTCTGCCAG CCAGCCATGC CATGCGTGTG 
TACGAGATGC TGATTAATCA TATCCAGCTT CACTACAAAT ACAATTACAG CTCACCCATT 
GCTACCAGTA TACGCCTCCA GGTTTTTGAC TTCCTCCTAC ACCTCCGAGC TGACTCTTTG 
CGGCGTGTTG GGCTACCTAA CAAAGATGGC GCCTTCAGAT TCAGTCCCTA TTGTCTTTGT 
GAATCTGTAA ATGAACAAGA GAAGAGAGCA GCTGAACGCA AGCCAGCAGG CACCTTGTCA 
CCCCCCTCTG GTAGCCCCAG TGTCCCATCA CAGACTGTCA CTCCACGCAT GGGATTCTTG 
CCATTCTCAC TAGCATTTGG GGTTATCCTG CAGTGCCTAA AGCAGGAAAC TGACTGGAAA 
GTCCTGAAAC TAGTCCTCAA CAAGCTGCCA GAGTGTCTGC GTTACAAACT GCTCATTCTG 
TCATCTCCCT GCAATATAGA TCAACTGGCT GGCTCTCTCT GTGCTATGCT TACAGACAAG 
ACTGCAAGTG ATCGCCTTTA CATTACTCCC GATGGCTTCT CTCGTACTGA CCTTCAGCTG 
GCAGTGGTTC CTGTACTGAC AGCCTTAACC TCATACCACC AACACCTAGA GCTCTCTAAA 
CAGAGGGAAA TGGTAAGGTG TCTGGAAACA GGCCTGATCT TTCGCTGCGG CAAGCAGTGC 
GTTGTGGCCC TTTCCATGTG TGTGATCGAG ATGCCAAGCA TTCTGATAAA ATCCCTCCCG 
GTCCTTATCG TGAAGCTAAC CCATATCTCG GCCACTGTCA CCATGGCCAT ACCAATGCTG 
GAGTTTCTTT CAACTTTGGT CCGCCTGCCC CACTTATATG CAAACTTTGC CGCAGAGCAG 
TATGCCAGTG TGTTTGCCAT CTCCCTTCCC TACACCAACC CATCAAAGTT TAATCAATAC 
ATTGTATTCC TTGCACACCA TGTCATTGCC ATGTGGTTCA TCCGGTGCAG ACTATCCCTA 
CGGAGAGACT TTGTGCCATA TATAACAAAG GGTTTGCGAT CCAATGTCCT GCTGAGCTTT 
GATGACACAC CGGAGAAGGG CAGCTTTAGA GCTCGCAGCA CCAGCTTGAA CGAGAGACCC 
AAAAGCCGTA TGCAGACTTC TGTCACCAGC TCCAGCTTGA GTTCAGCTGA TGAGAGCTCT 
ATGGCCCAGG CTGATGACAG CCTGAAAAAC CTTCACCTGG AATTGACCGA AATCTGCCTG 
GATATGATGG CACGATACGC CTTTGCCAAC TTCACAGCCG TGCCCAAAAG GTCGCCTGTA 
GGTGAGTTCC TGCTGGATGG CGGAAGAACG AAGACCTGGC TAGTTGGGAA CAAGCTGGTT 
ACCATTACAA CAAGTGTGGG CATGGGGACA CGTTCTCTTC TTGGCCTGGA TACAGGAGGG 
GAGAACCATG GTGACACTGA GAACAGCTCC CCCGTGCAAA CAGAGCAAGT CAAGGAGTTT 
CCGTCCATGC TCGATCTTCA GCAAAGAAAG CCGGGTATGC AAACGCCCCG TCACAGAGTC 
CGCTCCATGT CTGGAGGGAC TACACTGAGA GCTGGATCAC TAGAGGGGAC TCCTCTTTCG 
GCCAGTATGG GGCAGCCGGG GGCCACTCCA GTGACCAGAG CAGAGAAACC TTCCCGTAGG 
ACACAGATGA AGAAAGAAAA GACCACCCTG GCTGACTTTG TTCCCGTGCT GACACAAGGC 
TGGGCAGAGG TATTGGTTCG CAGACCCACA GGTAACACCA GCTGGTTGAT GAGCCTGGAA 
AATCCTCTGA GTCCCTTCTC TTCTGACATA AACAACATGC CGCTGCAGGA GCTTTCTAAT 
GCCCTAATGG CAGCCGAGAG GCTCAAGGAG CATCGGGAGA CAGCGCTCTA CAAATCTCTC 
TCTGTGCCAG CCTCAACTTT ATCACCCTCA ACAACCAAGT GTACCCTTCT ACAGCGGGCA 
AACACAGTGG CCTCTGTGTC TCTCGTAACC CAGTCCAGTG GTCAAGGAAA GCTGCTTAGG 
AGCATTTCCT GGGCAGAATC TTGTGCTGTG CCAGAGGAAG GGGCGCAAGC TGATGGGGAA 
GCAGAGGAAG TGTCCACTCC CGGGGGCTCT CAAGAGCTGG AAGATTTTGA GGCTGTGGTT 
AGCGAAGACA CCACTAGTGA AGAGAAGAGT GCTAAGAAGA TGGAGTGCAT TAGCAGGTCT 
TCATCCACAT CCAGTCAGGA AGAGTCTGCT GCTACCCAAA CGGAGGAATC CGAGGCTGGT 
GGAATACCCA TTGAGGGCTC CACTCGGGAG GTCCCATTTC AGACTTTGAG CAAGTCCAGC 
TCATCTCCTG AACTTCAGAC CTTGCAGGAG CTTCCTAAAG AGTTGGGCCC TACAGAAGAT 
ATAAAGAAGC CTTCTGCAGA CTTCAAAACA CTACCCCATG TAGAAAACCC TTTAAGCCAC 
AGAACTAAAT CTGAAGAGGA ACGCAGTGCT GGGGCATTAG CCAAACCAGA AGGCGAAATG 
CGACTTGAAC CAGAGTTGCC TGTTCTGTCC CCACAATCTC CAACTGGGCA CCGCCCAAGG 
GGTCACACTA TCTCTGATTC TGAGCCGTCC CGCAGAGGCA GGAGATTTCA GGTTGATCCT 
TTTAAGAACA GCACCAAATC CACCAAAGCT GAGAAAGTGC CTGGAATAGA TCCAAGCTTT 
GTATTTTTGC AGCTGTACCA TTCCCCATTC TTTGGTGATG AGTCCAATAA GCCTATACTG 
GTCCCTAACA CACAGACGTT TGAGAGAACA ATGGGCCTCC TAGATCGAAT CCCGCCCTAT 
GACACCCACA AAATTGGTGT TCTTTATGTT GGAGAGGGGC AGGTTGGCAA CGAGCGCGCA 
ATCCTATCCA ATGAGCATGG CTCGTACCGC TACACACAGT TCCTCACGGG GCTTGGGAAG 
CTCATCGAGC TGAAAGACAC TCAGCCAGAC AAGATCTTCC TGGGAGGGTT GGACGGTTGT 
GGAGATGACG GGCAGTTCAC CTACTGCTGG CATGATGATA TTATGCAAGT CATTTTTCAG 
ATCACCACCC TGATGCCAAA CAAAGAGATA GATCCGAACC GCTGCAACAA GAAAAGGCAC 
GTGGGCAACA ACTTTGTCAA TATCATCTAC AACGACTCGG GCGAGTTCTA CAAGTTGGGC 
ACCCTCAAGG GTCAGTTCAA CTTTGTAGAT GTCAACATTC AGCCTTTGGA CTGCGAGAGC 
AACCTGGTGA CACTGCAATG CCGAAAAGAT ATGGAAGGCC TTGTAGATAC CAGCGTGGCA 
AAAATTGTGT CTGACAAAAA CCTACCCTTC CTGGCCCGCC AGATGGCTCT ACATTCAAAT 
ATGGCTTCAC AAGTCCATCA CAGCAGGTCC AACCCCACAG ATATTTACCC TTCTAAATGG 
ATTGCCAGGC TGCGACACAT AAAGAAGATG AGACCAAAGA TACAAGAGGA GTTGCAGGAG 
CTGAGTGCCA ACATGCCCTA CCAGGTCCCT ACTATGGCCA ACAAGCCCCC TTCACAAAAC 
CCACAGACTC CCCCCGGCGC ATTTGAAACC GGCCAAAGGA CAAGGCTCAT TTCTGCTGTG 
GACGATTTCA CGGAGTTTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( NM_001130323.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123795.1 Xenopus tropicalis TSC complex subunit 2 (tsc2), mRNA.

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CloneID	OXa22998
Clone ID Related Accession (Same CDS sequence)	NM_001130323.1
Accession Version	NM_001130323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tuberin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167586.1. ##Evidence-Data-START## Transcript exon combination :: BC167586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028297, SAMD00028300 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGCAAT TTTTCTGGTG TGACTTTCCC 
CAGGGTGAAA GACTTGGCGT CCTTCGTGCC CATTTCTTCC GAGTCATAAA AGAGTATCCT 
ATGAATGAGG ACCTTGCAGA GCGACTGGAG GTTTTTAAAG CTCTCACCGA CAATGGGCGA 
GAAATTGCTT ACTTAGAAGA AGAGCTCGCT GTCTTCGTCC TCCAGTGGAT GGAAATTGGT 
TTGACTCCGG ATTTCCTGCA TGTTTTAGTT AACCTGGTTA AGTTCAATAG CTGTTACTTG 
GAACACTTTA TTGCTCCTAT GGTGCAGATG ATATGCCTCC TCTGTAATCG CACGACATCT 
CCTTACGATA TTGAGGTTTC CTTGAAGGTT CTGGATGCAG TGGTCTGCTA TAACTGCCTT 
CCATCAGAGA GCATTCCTAT ATTTGTTATG ACCCTGTGCC GCACAATAAA TGTTAAGGAG 
CTGTGTGAAC CATGCTGGAA GCTGATGCGA AACCTTCTGG GCACCCATCT TGGGCACAGC 
GCCATTTACA CTATGAGTCG TATAATGGAG GATAGAGCCC ACATGGAAGA TGCCCCTTTG 
CTAAGAGGAG CTGTGTTCTT TGTTGGGATG GGTCTCTGGG GAGCTCACAG ACTCTTCTGT 
CTGAAGAACT CCCCGATATC TGTTCTCCCA TCTTTCCTGA AGGCTATGAA CTGTAACAAT 
GCCGTTGTCT CCTATGAGAT TGTCCTCTCT GTCACTCGGC TAATCAAGAA GTACGGCAAG 
GATGTGCAGG CTGCCACGTG GGACGTTCTC CTTGATATCA TCGAGCGATT ATTCCAGCAG 
CTTCAGGTGC TGCGGAGTCA GGAATTAAAG GCTGCGGTGC ACAATCTCAT GACCACCACA 
GAGGAGCTTT ATAACCAGAA CGGATTCCAT GGAGATGAAG AGAGATTTTT CGAGCTGGTT 
GAAAAAGGGG CAGATGAAAG ACCTTTTGCA TCTGTTGTGA ACCTTATCAC CTACAGAGCT 
CAGTCCATTC ACCCTGGGAA GGAAGGGTGG ATTATGAATC TACAGAAACT GATGGATAGA 
TATTTCAGGA GCGAGTCTCG CAGTGTTGTA CGCATGAAAA TCTTAGATGT TCTGTCCTTT 
GCGTTGAGCA TCAATCGCCA GTTTTACGAG GATGAGTTGA TCGAGAAGGT AGTTACTTGC 
CAGCTAGCCC ATATACCCGA GGATAAGGAC CACCAGGTGC GCAAACTCGC AACACAGCTC 
CTTGTGGACC TGGCTGAATG CTGTCACTCC TGCCACTTCA ATAGTCTCAT GGATATCATT 
GAGAAGGTGG TGAACCGTTC CTTGTCCACA CCAGAGTCAG GTCATGATTC TTCTTCTTTG 
GAAGATGTGA AAACCTCTGT TCTGGGGCTG ATGGTTATTC TACAGACCAA GCTATACATT 
CTGCCAGCCA GCCATGCCAT GCGTGTGTAC GAGATGCTGA TTAATCATAT CCAGCTTCAC 
TACAAATACA ATTACAGCTC ACCCATTGCT ACCAGTATAC GCCTCCAGGT TTTTGACTTC 
CTCCTACACC TCCGAGCTGA CTCTTTGCGG CGTGTTGGGC TACCTAACAA AGATGGCGCC 
TTCAGATTCA GTCCCTATTG TCTTTGTGAA TCTGTAAATG AACAAGAGAA GAGAGCAGCT 
GAACGCAAGC CAGCAGGCAC CTTGTCACCC CCCTCTGGTA GCCCCAGTGT CCCATCACAG 
ACTGTCACTC CACGCATGGG ATTCTTGCCA TTCTCACTAG CATTTGGGGT TATCCTGCAG 
TGCCTAAAGC AGGAAACTGA CTGGAAAGTC CTGAAACTAG TCCTCAACAA GCTGCCAGAG 
TGTCTGCGTT ACAAACTGCT CATTCTGTCA TCTCCCTGCA ATATAGATCA ACTGGCTGGC 
TCTCTCTGTG CTATGCTTAC AGACAAGACT GCAAGTGATC GCCTTTACAT TACTCCCGAT 
GGCTTCTCTC GTACTGACCT TCAGCTGGCA GTGGTTCCTG TACTGACAGC CTTAACCTCA 
TACCACCAAC ACCTAGAGCT CTCTAAACAG AGGGAAATGG TAAGGTGTCT GGAAACAGGC 
CTGATCTTTC GCTGCGGCAA GCAGTGCGTT GTGGCCCTTT CCATGTGTGT GATCGAGATG 
CCAAGCATTC TGATAAAATC CCTCCCGGTC CTTATCGTGA AGCTAACCCA TATCTCGGCC 
ACTGTCACCA TGGCCATACC AATGCTGGAG TTTCTTTCAA CTTTGGTCCG CCTGCCCCAC 
TTATATGCAA ACTTTGCCGC AGAGCAGTAT GCCAGTGTGT TTGCCATCTC CCTTCCCTAC 
ACCAACCCAT CAAAGTTTAA TCAATACATT GTATTCCTTG CACACCATGT CATTGCCATG 
TGGTTCATCC GGTGCAGACT ATCCCTACGG AGAGACTTTG TGCCATATAT AACAAAGGGT 
TTGCGATCCA ATGTCCTGCT GAGCTTTGAT GACACACCGG AGAAGGGCAG CTTTAGAGCT 
CGCAGCACCA GCTTGAACGA GAGACCCAAA AGCCGTATGC AGACTTCTGT CACCAGCTCC 
AGCTTGAGTT CAGCTGATGA GAGCTCTATG GCCCAGGCTG ATGACAGCCT GAAAAACCTT 
CACCTGGAAT TGACCGAAAT CTGCCTGGAT ATGATGGCAC GATACGCCTT TGCCAACTTC 
ACAGCCGTGC CCAAAAGGTC GCCTGTAGGT GAGTTCCTGC TGGATGGCGG AAGAACGAAG 
ACCTGGCTAG TTGGGAACAA GCTGATTACC ATTACAACAA GTGTGGGCAT GGGGACACGT 
TCTCTTCTTG GCCTGGATAC AGGAGGGGAG AACCATGGTG ACACTGAGAA CAGCTCCCCC 
GTGCAAACAG AGCAAGTCAA GGAGTTTCCG TCCATGCTCG ATCTTCAGCA AAGAAAGCCG 
GGTATGCAAA CGCCCCGTCA CAGAGTCCGC TCCATGTCTG GAGGGACTAC ACTGAGAGCT 
GGATCACTAG AGGGGACTCC TCTTTCGGCC AGTATGGGGC AGCCGGGGGC CACTCCAGTG 
ACCAGAGCAG AGAAACCTTC CCGTAGGACA CAGATGAAGA AAGAAAAGAC CACCCTGGCT 
GACTTTGTTC CCGTGCTGAC ACAAGGCTGG GCAGAGGTAT TGGTTCGCAG ACCCACAGGT 
AACACCAGCT GGTTGATGAG CCTGGAAAAT CCTCTGAGCC CCTTCTCTTC TGACATAAAC 
AACATGCCGC TGCAGGAGCT TTCTAATGCC CTAATGGCAG CCGAGAGGCT CAAGGAGCAT 
CGGGAGACAG CGCTCTACAA ATCTCTCTCT GTGCCAGCTT CAACTTTATC ACCCTCAACA 
ACCAAGTGTA CCCTTCTACA GCGGGCAAAC ACAGAATCTT GTGCTGTGCC AGAGGAAGGG 
GCGCAAGCTG ATGGGGAAGC AGAGGAAGTG TCCACTCCCG GGGGCTCTCA AGAGCTGGAA 
GATTTTGAGG CTGTGGTTAG CGAAGACACC ACTAGTGAAG AGAAGAGTGC TAAGAAGATG 
GAGTGCATTA GCAGGTCTTC ATCCACATCC AGTCAGGAAG AGTCTGCTGC TACCCAAACG 
GAGGAATCCG AGGCTGGTGG AATACCCATT GAGGGCTCCA CTCGGGAGGT CCCATTTCAG 
ACTTTGAGCA AGTCCAGCTC ATCTCCTGAA CTTCAGACCT TGCAGGAGCT TCCTAAAGAG 
TTGGGCCCTA CAGAAGATAT AAAGAAGCCT TCTGCAGACT TCAAAACACT ACCCCATGTA 
GAAAACCCTT TAAGCCACAG AACTAAATCT GAAGAGGAAC GCAGTGCTGG GGCATTAGCC 
AAACCAGAAG GCGAAATGCG ACTTGAACCA GAGTTGCCTG TTCTGTCCCC ACAATCTCCA 
ACTGGGCACC GCCCAAGGGG TCACACTATC TCTGATTCTG AGCCGTCCCG CAGAGGCAGG 
AGATTTCAGG TTGATCCTTT TAAGAACAGC ACCAAATCCA CCAAAGCTGA GAAAGTGCCT 
GGAATAGATC CAAGCTTTGT ATTTTTGCAG CTGTACCATT CCCCATTCTT TGGTGATGAG 
TCCAATAAGC CTATACTGGT CCCTAACACA CAGACGTTTG AGAGAACAAT GGGCCTCCTA 
GATCGAATCC CGCCCTATGA CACCCACAAA ATTGGTGTTC TTTATGTTGG AGAGGGGCAG 
GTTGGCAACG AGCGCGCAAT CCTATCCAAT GAGCATGGCT CGTACCGCTA CACACAGTTC 
CTCACGGGGC TTGGGAAGCT CATCGAGCTG AAAGACACTC AGCCAGACAA GATCTTCCTG 
GGAGGGTTGG ACGGTTGTGG AGATGACGGG CAGTTCACCT ACTGCTGGCA TGATGATATT 
ATGCAAGTCA TTTTTCAGAT CACCACCCTG ATGCCAAACA AAGAGATAGA TCCGAACCGC 
TGCAACAAGA AAAGGCACGT GGGCAACAAC TTTGTCAATA TCATCTACAA CGACTCGGGC 
GAGTTCTACA AGTTGGGCAC CCTCAAGGGT CAGTTCAACT TTGTAGATGT CAACATTCAG 
CCTTTGGACT GCGAGAGCAA CCTGGTGACA CTGCAATGCC GAAAAGATAT GGAAGGCCTT 
GTAGATACCA GCGTGGCAAA AATTGTGTCT GACAAAAACC TACCCTTCCT GGCCCGCCAG 
ATGGCTCTAC ATTCAAATAT GGCTTCACAA GTCCATCACA GCAGGTCCAA CCCCACAGAT 
ATTTACCCTT CTAAATGGAT TGCCAGGCTG CGACACATAA AGAAGATGAG ACCAAAGATA 
CAAGAGGAGT TGCAGGAGCT GAGTGCCAAC ATGCCCTACC AGGTCCCTAC TATGGCCAAC 
AAGCCCCCTT CACAAAACCC ACAGACTCCC CCCGGCGCAT TTGAAACCGG CCAAAGGACA 
AGGCTCATTT CTGCTGTGGA CGATTTCACG GAGTTTGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123795.1
CDS	140..5401
Translation	MAKQMGKDPGLKEKVKMLLGLVPARVKTKPSEWKHPEYVITADLMKELSSESALHVRIRAIIQVCELAKTKKFEEHAVEAICQSVGDLIQPEQPVEARHHVLQLLKAIIVGQQFFWCDFPQGERLGVLRAHFFRVIKEYPMNEDLAERLEVFKALTDNGREIAYLEEELAVFVLQWMEIGLTPDFLHVLVNLVKFNSCYLEHFIAPMVQMICLLCNRTTSPYDIEVSLKVLDAVVCYNCLPSESIPIFVMTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYTMSRIMEDRAHMEDAPLLRGAVFFVGMGLWGAHRLFCLKNSPISVLPSFLKAMNCNNAVVSYEIVLSVTRLIKKYGKDVQAATWDVLLDIIERLFQQLQVLRSQELKAAVHNLMTTTEELYNQNGFHGDEERFFELVEKGADERPFASVVNLITYRAQSIHPGKEGWIMNLQKLMDRYFRSESRSVVRMKILDVLSFALSINRQFYEDELIEKVVTCQLAHIPEDKDHQVRKLATQLLVDLAECCHSCHFNSLMDIIEKVVNRSLSTPESGHDSSSLEDVKTSVLGLMVILQTKLYILPASHAMRVYEMLINHIQLHYKYNYSSPIATSIRLQVFDFLLHLRADSLRRVGLPNKDGAFRFSPYCLCESVNEQEKRAAERKPAGTLSPPSGSPSVPSQTVTPRMGFLPFSLAFGVILQCLKQETDWKVLKLVLNKLPECLRYKLLILSSPCNIDQLAGSLCAMLTDKTASDRLYITPDGFSRTDLQLAVVPVLTALTSYHQHLELSKQREMVRCLETGLIFRCGKQCVVALSMCVIEMPSILIKSLPVLIVKLTHISATVTMAIPMLEFLSTLVRLPHLYANFAAEQYASVFAISLPYTNPSKFNQYIVFLAHHVIAMWFIRCRLSLRRDFVPYITKGLRSNVLLSFDDTPEKGSFRARSTSLNERPKSRMQTSVTSSSLSSADESSMAQADDSLKNLHLELTEICLDMMARYAFANFTAVPKRSPVGEFLLDGGRTKTWLVGNKLITITTSVGMGTRSLLGLDTGGENHGDTENSSPVQTEQVKEFPSMLDLQQRKPGMQTPRHRVRSMSGGTTLRAGSLEGTPLSASMGQPGATPVTRAEKPSRRTQMKKEKTTLADFVPVLTQGWAEVLVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERLKEHRETALYKSLSVPASTLSPSTTKCTLLQRANTESCAVPEEGAQADGEAEEVSTPGGSQELEDFEAVVSEDTTSEEKSAKKMECISRSSSTSSQEESAATQTEESEAGGIPIEGSTREVPFQTLSKSSSSPELQTLQELPKELGPTEDIKKPSADFKTLPHVENPLSHRTKSEEERSAGALAKPEGEMRLEPELPVLSPQSPTGHRPRGHTISDSEPSRRGRRFQVDPFKNSTKSTKAEKVPGIDPSFVFLQLYHSPFFGDESNKPILVPNTQTFERTMGLLDRIPPYDTHKIGVLYVGEGQVGNERAILSNEHGSYRYTQFLTGLGKLIELKDTQPDKIFLGGLDGCGDDGQFTYCWHDDIMQVIFQITTLMPNKEIDPNRCNKKRHVGNNFVNIIYNDSGEFYKLGTLKGQFNFVDVNIQPLDCESNLVTLQCRKDMEGLVDTSVAKIVSDKNLPFLARQMALHSNMASQVHHSRSNPTDIYPSKWIARLRHIKKMRPKIQEELQELSANMPYQVPTMANKPPSQNPQTPPGAFETGQRTRLISAVDDFTEFA

Target ORF information:

RefSeq Version	NM_001130323.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis TSC complex subunit 2 (tsc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130323.1

ORF Insert Sequence:

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGCAAT TTTTCTGGTG TGACTTTCCC 
CAGGGTGAAA GACTTGGCGT CCTTCGTGCC CATTTCTTCC GAGTCATAAA AGAGTATCCT 
ATGAATGAGG ACCTTGCAGA GCGACTGGAG GTTTTTAAAG CTCTCACCGA CAATGGGCGA 
GAAATTGCTT ACTTAGAAGA AGAGCTCGCT GTCTTCGTCC TCCAGTGGAT GGAAATTGGT 
TTGACTCCGG ATTTCCTGCA TGTTTTAGTT AACCTGGTTA AGTTCAATAG CTGTTACTTG 
GAACACTTTA TTGCTCCTAT GGTGCAGATG ATATGCCTCC TCTGTAATCG CACGACATCT 
CCTTACGATA TTGAGGTTTC CTTGAAGGTT CTGGATGCAG TGGTCTGCTA TAACTGCCTT 
CCATCAGAGA GCATTCCTAT ATTTGTTATG ACCCTGTGCC GCACAATAAA TGTTAAGGAG 
CTGTGTGAAC CATGCTGGAA GCTGATGCGA AACCTTCTGG GCACCCATCT TGGGCACAGC 
GCCATTTACA CTATGAGTCG TATAATGGAG GATAGAGCCC ACATGGAAGA TGCCCCTTTG 
CTAAGAGGAG CTGTGTTCTT TGTTGGGATG GGTCTCTGGG GAGCTCACAG ACTCTTCTGT 
CTGAAGAACT CCCCGATATC TGTTCTCCCA TCTTTCCTGA AGGCTATGAA CTGTAACAAT 
GCCGTTGTCT CCTATGAGAT TGTCCTCTCT GTCACTCGGC TAATCAAGAA GTACGGCAAG 
GATGTGCAGG CTGCCACGTG GGACGTTCTC CTTGATATCA TCGAGCGATT ATTCCAGCAG 
CTTCAGGTGC TGCGGAGTCA GGAATTAAAG GCTGCGGTGC ACAATCTCAT GACCACCACA 
GAGGAGCTTT ATAACCAGAA CGGATTCCAT GGAGATGAAG AGAGATTTTT CGAGCTGGTT 
GAAAAAGGGG CAGATGAAAG ACCTTTTGCA TCTGTTGTGA ACCTTATCAC CTACAGAGCT 
CAGTCCATTC ACCCTGGGAA GGAAGGGTGG ATTATGAATC TACAGAAACT GATGGATAGA 
TATTTCAGGA GCGAGTCTCG CAGTGTTGTA CGCATGAAAA TCTTAGATGT TCTGTCCTTT 
GCGTTGAGCA TCAATCGCCA GTTTTACGAG GATGAGTTGA TCGAGAAGGT AGTTACTTGC 
CAGCTAGCCC ATATACCCGA GGATAAGGAC CACCAGGTGC GCAAACTCGC AACACAGCTC 
CTTGTGGACC TGGCTGAATG CTGTCACTCC TGCCACTTCA ATAGTCTCAT GGATATCATT 
GAGAAGGTGG TGAACCGTTC CTTGTCCACA CCAGAGTCAG GTCATGATTC TTCTTCTTTG 
GAAGATGTGA AAACCTCTGT TCTGGGGCTG ATGGTTATTC TACAGACCAA GCTATACATT 
CTGCCAGCCA GCCATGCCAT GCGTGTGTAC GAGATGCTGA TTAATCATAT CCAGCTTCAC 
TACAAATACA ATTACAGCTC ACCCATTGCT ACCAGTATAC GCCTCCAGGT TTTTGACTTC 
CTCCTACACC TCCGAGCTGA CTCTTTGCGG CGTGTTGGGC TACCTAACAA AGATGGCGCC 
TTCAGATTCA GTCCCTATTG TCTTTGTGAA TCTGTAAATG AACAAGAGAA GAGAGCAGCT 
GAACGCAAGC CAGCAGGCAC CTTGTCACCC CCCTCTGGTA GCCCCAGTGT CCCATCACAG 
ACTGTCACTC CACGCATGGG ATTCTTGCCA TTCTCACTAG CATTTGGGGT TATCCTGCAG 
TGCCTAAAGC AGGAAACTGA CTGGAAAGTC CTGAAACTAG TCCTCAACAA GCTGCCAGAG 
TGTCTGCGTT ACAAACTGCT CATTCTGTCA TCTCCCTGCA ATATAGATCA ACTGGCTGGC 
TCTCTCTGTG CTATGCTTAC AGACAAGACT GCAAGTGATC GCCTTTACAT TACTCCCGAT 
GGCTTCTCTC GTACTGACCT TCAGCTGGCA GTGGTTCCTG TACTGACAGC CTTAACCTCA 
TACCACCAAC ACCTAGAGCT CTCTAAACAG AGGGAAATGG TAAGGTGTCT GGAAACAGGC 
CTGATCTTTC GCTGCGGCAA GCAGTGCGTT GTGGCCCTTT CCATGTGTGT GATCGAGATG 
CCAAGCATTC TGATAAAATC CCTCCCGGTC CTTATCGTGA AGCTAACCCA TATCTCGGCC 
ACTGTCACCA TGGCCATACC AATGCTGGAG TTTCTTTCAA CTTTGGTCCG CCTGCCCCAC 
TTATATGCAA ACTTTGCCGC AGAGCAGTAT GCCAGTGTGT TTGCCATCTC CCTTCCCTAC 
ACCAACCCAT CAAAGTTTAA TCAATACATT GTATTCCTTG CACACCATGT CATTGCCATG 
TGGTTCATCC GGTGCAGACT ATCCCTACGG AGAGACTTTG TGCCATATAT AACAAAGGGT 
TTGCGATCCA ATGTCCTGCT GAGCTTTGAT GACACACCGG AGAAGGGCAG CTTTAGAGCT 
CGCAGCACCA GCTTGAACGA GAGACCCAAA AGCCGTATGC AGACTTCTGT CACCAGCTCC 
AGCTTGAGTT CAGCTGATGA GAGCTCTATG GCCCAGGCTG ATGACAGCCT GAAAAACCTT 
CACCTGGAAT TGACCGAAAT CTGCCTGGAT ATGATGGCAC GATACGCCTT TGCCAACTTC 
ACAGCCGTGC CCAAAAGGTC GCCTGTAGGT GAGTTCCTGC TGGATGGCGG AAGAACGAAG 
ACCTGGCTAG TTGGGAACAA GCTGATTACC ATTACAACAA GTGTGGGCAT GGGGACACGT 
TCTCTTCTTG GCCTGGATAC AGGAGGGGAG AACCATGGTG ACACTGAGAA CAGCTCCCCC 
GTGCAAACAG AGCAAGTCAA GGAGTTTCCG TCCATGCTCG ATCTTCAGCA AAGAAAGCCG 
GGTATGCAAA CGCCCCGTCA CAGAGTCCGC TCCATGTCTG GAGGGACTAC ACTGAGAGCT 
GGATCACTAG AGGGGACTCC TCTTTCGGCC AGTATGGGGC AGCCGGGGGC CACTCCAGTG 
ACCAGAGCAG AGAAACCTTC CCGTAGGACA CAGATGAAGA AAGAAAAGAC CACCCTGGCT 
GACTTTGTTC CCGTGCTGAC ACAAGGCTGG GCAGAGGTAT TGGTTCGCAG ACCCACAGGT 
AACACCAGCT GGTTGATGAG CCTGGAAAAT CCTCTGAGCC CCTTCTCTTC TGACATAAAC 
AACATGCCGC TGCAGGAGCT TTCTAATGCC CTAATGGCAG CCGAGAGGCT CAAGGAGCAT 
CGGGAGACAG CGCTCTACAA ATCTCTCTCT GTGCCAGCTT CAACTTTATC ACCCTCAACA 
ACCAAGTGTA CCCTTCTACA GCGGGCAAAC ACAGAATCTT GTGCTGTGCC AGAGGAAGGG 
GCGCAAGCTG ATGGGGAAGC AGAGGAAGTG TCCACTCCCG GGGGCTCTCA AGAGCTGGAA 
GATTTTGAGG CTGTGGTTAG CGAAGACACC ACTAGTGAAG AGAAGAGTGC TAAGAAGATG 
GAGTGCATTA GCAGGTCTTC ATCCACATCC AGTCAGGAAG AGTCTGCTGC TACCCAAACG 
GAGGAATCCG AGGCTGGTGG AATACCCATT GAGGGCTCCA CTCGGGAGGT CCCATTTCAG 
ACTTTGAGCA AGTCCAGCTC ATCTCCTGAA CTTCAGACCT TGCAGGAGCT TCCTAAAGAG 
TTGGGCCCTA CAGAAGATAT AAAGAAGCCT TCTGCAGACT TCAAAACACT ACCCCATGTA 
GAAAACCCTT TAAGCCACAG AACTAAATCT GAAGAGGAAC GCAGTGCTGG GGCATTAGCC 
AAACCAGAAG GCGAAATGCG ACTTGAACCA GAGTTGCCTG TTCTGTCCCC ACAATCTCCA 
ACTGGGCACC GCCCAAGGGG TCACACTATC TCTGATTCTG AGCCGTCCCG CAGAGGCAGG 
AGATTTCAGG TTGATCCTTT TAAGAACAGC ACCAAATCCA CCAAAGCTGA GAAAGTGCCT 
GGAATAGATC CAAGCTTTGT ATTTTTGCAG CTGTACCATT CCCCATTCTT TGGTGATGAG 
TCCAATAAGC CTATACTGGT CCCTAACACA CAGACGTTTG AGAGAACAAT GGGCCTCCTA 
GATCGAATCC CGCCCTATGA CACCCACAAA ATTGGTGTTC TTTATGTTGG AGAGGGGCAG 
GTTGGCAACG AGCGCGCAAT CCTATCCAAT GAGCATGGCT CGTACCGCTA CACACAGTTC 
CTCACGGGGC TTGGGAAGCT CATCGAGCTG AAAGACACTC AGCCAGACAA GATCTTCCTG 
GGAGGGTTGG ACGGTTGTGG AGATGACGGG CAGTTCACCT ACTGCTGGCA TGATGATATT 
ATGCAAGTCA TTTTTCAGAT CACCACCCTG ATGCCAAACA AAGAGATAGA TCCGAACCGC 
TGCAACAAGA AAAGGCACGT GGGCAACAAC TTTGTCAATA TCATCTACAA CGACTCGGGC 
GAGTTCTACA AGTTGGGCAC CCTCAAGGGT CAGTTCAACT TTGTAGATGT CAACATTCAG 
CCTTTGGACT GCGAGAGCAA CCTGGTGACA CTGCAATGCC GAAAAGATAT GGAAGGCCTT 
GTAGATACCA GCGTGGCAAA AATTGTGTCT GACAAAAACC TACCCTTCCT GGCCCGCCAG 
ATGGCTCTAC ATTCAAATAT GGCTTCACAA GTCCATCACA GCAGGTCCAA CCCCACAGAT 
ATTTACCCTT CTAAATGGAT TGCCAGGCTG CGACACATAA AGAAGATGAG ACCAAAGATA 
CAAGAGGAGT TGCAGGAGCT GAGTGCCAAC ATGCCCTACC AGGTCCCTAC TATGGCCAAC 
AAGCCCCCTT CACAAAACCC ACAGACTCCC CCCGGCGCAT TTGAAACCGG CCAAAGGACA 
AGGCTCATTT CTGCTGTGGA CGATTTCACG GAGTTTGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_012970165.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825619.1 Xenopus tropicalis tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.

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CloneID	OXa32398
Clone ID Related Accession (Same CDS sequence)	XM_012970165.2
Accession Version	XM_012970165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGGGTG AAAGACTTGG CGTCCTTCGT 
GCCCATTTCT TCCGAGTCAT AAAAGAGTAT CCTATGAATG AGGACCTTGC AGAGCGACTG 
GAGGTTTTTA AAGCTCTCAC CGACAATGGG CGAGAAATTG CTTACTTAGA AGAAGAGCTC 
GCTGTCTTCG TCCTCCAGTG GATGGAAATT GGTTTGACTC CGGATTTCCT GCATGTTTTA 
GTTAACCTGG TTAAGTTCAA TAGCTGTTAC TTGGAACACT TTATTGCTCC TATGGTGCAG 
ATGATATGCC TCCTCTGTAA TCGCACGACA TCTCCTTACG ATATTGAGGT TTCCTTGAAG 
GTTCTGGATG CAGTGGTCTG CTATAACTGC CTTCCATCAG AGAGCATTCC TATATTTGTT 
ATGACCCTGT GCCGCACAAT AAATGTTAAG GAGCTGTGTG AACCATGCTG GAAGCTGATG 
CGAAACCTTC TGGGCACCCA TCTTGGGCAC AGCGCCATTT ACACTATGAG TCGTATAATG 
GAGGATAGAG CCCACATGGA AGATGCCCCT TTGCTAAGAG GAGCTGTGTT CTTTGTTGGG 
ATGGGTCTCT GGGGAGCTCA CAGACTCTTC TGTCTGAAGA ACTCCCCGAT ATCTGTTCTC 
CCATCTTTCC TGAAGGCTAT GAACTGTAAC AATGCCGTTG TCTCCTATGA GATTGTCCTC 
TCTGTCACTC GGCTAATCAA GAAGTACGGC AAGGATGTGC AGGCTGCCAC GTGGGACGTT 
CTCCTTGATA TCATTGAGCG ATTATTCCAG CAGCTTCAGG TGCTGCGGAG TCAGGAATTA 
AAGGCTGCGG TGCACAATCT CATGACCACC ACAGAGGAGC TTTATAACCA GAACGGATTC 
CATGGAGATG AAGAGAGATT TTTCGAGCTG GTTGAAAAAG GGGCAGATGA AAGACCTTTT 
GCATCAGTTG TGAACCTTAT CACCTACAGA GCTCAGTCCA TTCACCCTGG GAAGGAAGGG 
TGGATTATGA ATCTACAGAA ACTGATGGAT AGATATTTCA GGAGCGAGTC TCGCAGTGTT 
GTACGCATGA AAATCTTAGA TGTTCTGTCC TTTGCGTTGA GCATCAATCG CCAGTTTTAC 
GAGGATGAGT TGATCGAGAA GGTAGTTACT TGCCAGCTAG CCCATATACC CGAGGATAAG 
GACCACCAGG TGCGCAAACT CGCAACACAG CTCCTTGTGG ACCTGGCTGA ATGCTGTCAC 
TCCTGCCACT TCAATAGTCT CATGGATATC ATTGAGAAGG TGGTGAACCG TTCCTTGTCC 
ACACCAGAGT CAGGTCATGA TTCTTCTTCT TTGGAAGATG TGAAAACCTC TGTTCTGGGG 
CTGATGGTTA TTCTACAGAC CAAGCTATAC ATTCTGCCAG CCAGCCATGC CATGCGTGTG 
TACGAGATGC TGATTAATCA TATCCAGCTT CACTACAAAT ACAATTACAG CTCACCCATT 
GCTACCAGTA TACGCCTCCA GGTTTTTGAC TTCCTCCTAC ACCTCCGAGC TGACTCTTTG 
CGGCGTGTTG GGCTACCTAA CAAAGATGGC GCCTTCAGAT TCAGTCCCTA TTGTCTTTGT 
GAATCTGTAA ATGAACAAGA GAAGAGAGCA GCTGAACGCA AGCCAGCAGG CACCTTGTCA 
CCCCCCTCTG GTAGCCCCAG TGTCCCATCA CAGACTGTCG CTCCACGCAT GGGATTCTTG 
CCATTCTCAC TAGCATTTGG GGTTATCCTG CAGTGCCTAA AGCAGGAAAC TGACTGGAAA 
GTCCTGAAAC TAGTCCTCAA CAAGCTGCCA GAGTGTCTGC GTTACAAACT GCTCATTCTG 
TCATCTCCCT GCAATATAGA TCAACTGGCT GGCTCTCTCT GTGCTATGCT TACAGACAAG 
ACTGCAAGTG ATCGCCTTTA CATTACTCCC GATGGCTTCT CTCGTACTGA CCTTCAGCTG 
GCAGTGGTTC CTGTACTGAC AGCCTTAACC TCATACCACC AACACCTAGA GCTCTCTAAA 
CAGAGGGAAA TGGTAAGGTG TCTGGAAACA GGCCTGATCT TTCGCTGCGG CAAGCAGTGC 
GTTGTGGCCC TTTCCATGTG TGTGATCGAG ATGCCAAGCA TTCTGATAAA ATCCCTCCCG 
GTCCTTATCG TGAAGCTAAC CCATATCTCG GCCACTGTCA CCATGGCCAT ACCAATGCTG 
GAGTTTCTTT CAACTTTGGT CCGCCTGCCC CACTTATATG CAAACTTTGC CGCAGAGCAG 
TATGCCAGTG TGTTTGCCAT CTCCCTTCCC TACACCAACC CATCAAAGTT TAATCAATAC 
ATTGTATTCC TTGCACACCA TGTCATTGCC ATGTGGTTCA TCCGGTGCAG ACTATCCCTA 
CGGAGAGACT TTGTGCCATA TATAACAAAG GGTTTGCGAT CCAATGTCCT GCTGAGCTTT 
GATGACACAC CGGAGAAGGG CAGCTTTAGA GCTCGCAGCA CCAGCTTGAA CGAGAGACCC 
AAAAGCTTGA AGCTATCTAA ACACACAAAG CCTGGCTTCA GTGACACGTC CCCAGTTAAG 
GAGATGAGAG ATCTCTCCAA TGCCTTCCGC TCTCGCAGCA TCAGCGTGTC GGAGCATGCT 
GCGTACAGCC GTATGCAGAC TTCTGTCACC AGCTCCAGCT TGAGTTCAGC TGATGAGAGC 
TCTATGGCCC AGGCTGATGA CAGCCTGAAA AACCTTCACC TGGAATTGAC CGAAATCTGC 
CTGGATATGA TGGCACGATA CGCCTTTGCC AACTTCACAG CCGTGCCCAA AAGGTCGCCT 
GTAGGTGAGT TCCTGCTGGA TGGCGGAAGA ACGAAGACCT GGCTAGTTGG GAACAAGCTG 
ATTACCATTA CAACAAGTGT GGGCATGGGG ACACGTTCTC TTCTTGGCCT GGATACAGGA 
GGGGAGAACC ATGGTGACAC TGAGAACAGC TCCCCCGTGC AAACAGAGCA AGTCAAGGAG 
TTTCCGTCCA TGCTCGATCT TCAGCAAAGA AAGCCGGGTA TGCAAACGCC CCGTCACAGA 
GTCCGCTCCA TGTCTGGAGG GACTACACTG AGAGCTGGAT CACTAGAGGG GACTCCTCTT 
TCGGCCAGTA TGGGGCAGCC GGGGGCCACT CCAGTGACCA GAGCAGAGAA ACCTTCCCGT 
AGGACACAGA TGAAGAAAGA AAAGACCACC CTGGCTGACT TTGTTCCCGT GCTGACACAA 
GGCTGGGCAG AGGTATTGGT TCGCAGACCC ACAGGTAACA CCAGCTGGTT GATGAGCCTG 
GAAAATCCTC TGAGCCCCTT CTCTTCTGAC ATAAACAACA TGCCGCTGCA GGAGCTTTCT 
AATGCCCTAA TGGCAGCCGA GAGGCTCAAG GAGCATCGGG AGACAGCGCT CTACAAATCT 
CTCTCTGTGC CAGCTTCAAC TTTATCACCC TCAACAACCA AGTGTACCCT TCTACAGCGG 
GCAAACACAG TGGCCTCTGT GTCTCTCGTA ACCCAGTCCA GTGGTCAAGG AAAGCTGCTT 
AGGAGCATTT CCTGGGCAGA ATCTTGTGCT GTGCCAGAGG AAGGGGCGCA AGCTGATGGG 
GAAGCAGAGG AAGTGTCCAC TCCCGGGGGC TCTCAAGAGC TGGAAGATTT TGAGGCTGTG 
GTTAGCGAAG ACACCACTAG TGAAGAGAAG AGTGCTAAGA AGATGGAGTG CATTAGCAGG 
TCTTCATCCA CATCCAGTCA GGAAGAGTCT GCTGCTACCC AAACGGAGGA ATCCGAGGCT 
GGTGGAATAC CCATTGAGGG CTCCACTCGG GAGGTCCCAT TTCAGACTTT GAGCAAGTCC 
AGCTCATCTC CTGAACTTCA GACCTTGCAG GAGCTTCCTA AAGAGTTGGG CCCTACAGAA 
GATATAAAGA AGCCTTCTGC AGACTTCAAA ACACTACCCC ATGTAGAAAA CCCTTTAAGC 
CACAGAACTA AATCTGAAGA GGAACGCAGT GCTGGGGCAT TAGCCAAACC AGAAGGCGAA 
ATGCGACTTG AACCAGAGTT GCCTGTTCTG TCCCCACAAT CTCCAACTGG GCACCGCCCA 
AGGGGTCACA CTATCTCTGA TTCTGAGCCG TCCCGCAGAG GCAGGAGATT TCAGGTTGAT 
CCTTTTAAGA ACAGCACCAA ATCCACCAAA GCTGAGAAAG TGCCTGGAAT AGATCCAAGC 
TTTGTATTTT TGCAGCTGTA CCATTCCCCA TTCTTTGGTG ATGAGTCCAA TAAGCCTATA 
CTGGTCCCTA ACACACAGAC GTTTGAGAGA ACAATGGGCC TCCTAGATCG AATCCCGCCC 
TATGACACCC ACAAAATTGG TGTTCTTTAT GTTGGAGAGG GGCAGGTTGG CAACGAGCGC 
GCAATCCTAT CCAATGAGCA TGGCTCGTAC CGCTACACAC AGTTCCTCAC GGGGCTTGGG 
AAGCTCATCG AGCTGAAAGA CACTCAGCCA GACAAGATCT TCCTGGGAGG GTTGGACGGT 
TGTGGAGATG ACGGGCAGTT CACCTACTGC TGGCATGATG ATATTATGCA AGTCATTTTT 
CAGATCACCA CCCTGATGCC AAACAAAGAG ATAGATCCGA ACCGCTGCAA CAAGAAAAGG 
CACGTGGGCA ACAACTTTGT CAATATCATC TACAACGACT CGGGCGAGTT CTACAAGTTG 
GGCACCCTCA AGGGTCAGTT CAACTTTGTA GATGTCAACA TTCAGCCTTT GGACTGCGAG 
AGCAACCTGG TGACACTGCA ATGCCGAAAA GATATGGAAG GCCTTGTAGA TACCAGCGTG 
GCAAAAATTG TGTCTGACAA AAACCTACCC TTCCTGGCCC GCCAGATGGC TCTACATTCA 
AATATGGCTT CACAAGTCCA TCACAGCAGG TCCAACCCCA CAGATATTTA CCCTTCTAAA 
TGGATTGCCA GGCTGCGACA CATAAAGAAG ATGAGACCAA AGATACAAGA GGAGTTGCAG 
GAGCTGAGTG CCAACATGCC CTACCAGGTC CCTACTATGG CCAACAAGCC CCCTTCACAA 
AACCCACAGA CTCCCCCCGG CGCATTTGAA ACCGGCCAAA GGACAAGGCT CATTTCTGCT 
GTGGACGATT TCACGGAGTT TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825619.1
CDS	123..5549
Translation	MAKQMGKDPGLKEKVKMLLGLVPARVKTKPSEWKHPEYVITADLMKELSSESALHVRIRAIIQVCELAKTKKFEEHAVEAICQSVGDLIQPEQPVEARHHVLQLLKAIIVGQGERLGVLRAHFFRVIKEYPMNEDLAERLEVFKALTDNGREIAYLEEELAVFVLQWMEIGLTPDFLHVLVNLVKFNSCYLEHFIAPMVQMICLLCNRTTSPYDIEVSLKVLDAVVCYNCLPSESIPIFVMTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYTMSRIMEDRAHMEDAPLLRGAVFFVGMGLWGAHRLFCLKNSPISVLPSFLKAMNCNNAVVSYEIVLSVTRLIKKYGKDVQAATWDVLLDIIERLFQQLQVLRSQELKAAVHNLMTTTEELYNQNGFHGDEERFFELVEKGADERPFASVVNLITYRAQSIHPGKEGWIMNLQKLMDRYFRSESRSVVRMKILDVLSFALSINRQFYEDELIEKVVTCQLAHIPEDKDHQVRKLATQLLVDLAECCHSCHFNSLMDIIEKVVNRSLSTPESGHDSSSLEDVKTSVLGLMVILQTKLYILPASHAMRVYEMLINHIQLHYKYNYSSPIATSIRLQVFDFLLHLRADSLRRVGLPNKDGAFRFSPYCLCESVNEQEKRAAERKPAGTLSPPSGSPSVPSQTVAPRMGFLPFSLAFGVILQCLKQETDWKVLKLVLNKLPECLRYKLLILSSPCNIDQLAGSLCAMLTDKTASDRLYITPDGFSRTDLQLAVVPVLTALTSYHQHLELSKQREMVRCLETGLIFRCGKQCVVALSMCVIEMPSILIKSLPVLIVKLTHISATVTMAIPMLEFLSTLVRLPHLYANFAAEQYASVFAISLPYTNPSKFNQYIVFLAHHVIAMWFIRCRLSLRRDFVPYITKGLRSNVLLSFDDTPEKGSFRARSTSLNERPKSLKLSKHTKPGFSDTSPVKEMRDLSNAFRSRSISVSEHAAYSRMQTSVTSSSLSSADESSMAQADDSLKNLHLELTEICLDMMARYAFANFTAVPKRSPVGEFLLDGGRTKTWLVGNKLITITTSVGMGTRSLLGLDTGGENHGDTENSSPVQTEQVKEFPSMLDLQQRKPGMQTPRHRVRSMSGGTTLRAGSLEGTPLSASMGQPGATPVTRAEKPSRRTQMKKEKTTLADFVPVLTQGWAEVLVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERLKEHRETALYKSLSVPASTLSPSTTKCTLLQRANTVASVSLVTQSSGQGKLLRSISWAESCAVPEEGAQADGEAEEVSTPGGSQELEDFEAVVSEDTTSEEKSAKKMECISRSSSTSSQEESAATQTEESEAGGIPIEGSTREVPFQTLSKSSSSPELQTLQELPKELGPTEDIKKPSADFKTLPHVENPLSHRTKSEEERSAGALAKPEGEMRLEPELPVLSPQSPTGHRPRGHTISDSEPSRRGRRFQVDPFKNSTKSTKAEKVPGIDPSFVFLQLYHSPFFGDESNKPILVPNTQTFERTMGLLDRIPPYDTHKIGVLYVGEGQVGNERAILSNEHGSYRYTQFLTGLGKLIELKDTQPDKIFLGGLDGCGDDGQFTYCWHDDIMQVIFQITTLMPNKEIDPNRCNKKRHVGNNFVNIIYNDSGEFYKLGTLKGQFNFVDVNIQPLDCESNLVTLQCRKDMEGLVDTSVAKIVSDKNLPFLARQMALHSNMASQVHHSRSNPTDIYPSKWIARLRHIKKMRPKIQEELQELSANMPYQVPTMANKPPSQNPQTPPGAFETGQRTRLISAVDDFTEFA

Target ORF information:

RefSeq Version	XM_012970165.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tuberous sclerosis 2 (tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970165.2

ORF Insert Sequence:

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGGGTG AAAGACTTGG CGTCCTTCGT 
GCCCATTTCT TCCGAGTCAT AAAAGAGTAT CCTATGAATG AGGACCTTGC AGAGCGACTG 
GAGGTTTTTA AAGCTCTCAC CGACAATGGG CGAGAAATTG CTTACTTAGA AGAAGAGCTC 
GCTGTCTTCG TCCTCCAGTG GATGGAAATT GGTTTGACTC CGGATTTCCT GCATGTTTTA 
GTTAACCTGG TTAAGTTCAA TAGCTGTTAC TTGGAACACT TTATTGCTCC TATGGTGCAG 
ATGATATGCC TCCTCTGTAA TCGCACGACA TCTCCTTACG ATATTGAGGT TTCCTTGAAG 
GTTCTGGATG CAGTGGTCTG CTATAACTGC CTTCCATCAG AGAGCATTCC TATATTTGTT 
ATGACCCTGT GCCGCACAAT AAATGTTAAG GAGCTGTGTG AACCATGCTG GAAGCTGATG 
CGAAACCTTC TGGGCACCCA TCTTGGGCAC AGCGCCATTT ACACTATGAG TCGTATAATG 
GAGGATAGAG CCCACATGGA AGATGCCCCT TTGCTAAGAG GAGCTGTGTT CTTTGTTGGG 
ATGGGTCTCT GGGGAGCTCA CAGACTCTTC TGTCTGAAGA ACTCCCCGAT ATCTGTTCTC 
CCATCTTTCC TGAAGGCTAT GAACTGTAAC AATGCCGTTG TCTCCTATGA GATTGTCCTC 
TCTGTCACTC GGCTAATCAA GAAGTACGGC AAGGATGTGC AGGCTGCCAC GTGGGACGTT 
CTCCTTGATA TCATTGAGCG ATTATTCCAG CAGCTTCAGG TGCTGCGGAG TCAGGAATTA 
AAGGCTGCGG TGCACAATCT CATGACCACC ACAGAGGAGC TTTATAACCA GAACGGATTC 
CATGGAGATG AAGAGAGATT TTTCGAGCTG GTTGAAAAAG GGGCAGATGA AAGACCTTTT 
GCATCAGTTG TGAACCTTAT CACCTACAGA GCTCAGTCCA TTCACCCTGG GAAGGAAGGG 
TGGATTATGA ATCTACAGAA ACTGATGGAT AGATATTTCA GGAGCGAGTC TCGCAGTGTT 
GTACGCATGA AAATCTTAGA TGTTCTGTCC TTTGCGTTGA GCATCAATCG CCAGTTTTAC 
GAGGATGAGT TGATCGAGAA GGTAGTTACT TGCCAGCTAG CCCATATACC CGAGGATAAG 
GACCACCAGG TGCGCAAACT CGCAACACAG CTCCTTGTGG ACCTGGCTGA ATGCTGTCAC 
TCCTGCCACT TCAATAGTCT CATGGATATC ATTGAGAAGG TGGTGAACCG TTCCTTGTCC 
ACACCAGAGT CAGGTCATGA TTCTTCTTCT TTGGAAGATG TGAAAACCTC TGTTCTGGGG 
CTGATGGTTA TTCTACAGAC CAAGCTATAC ATTCTGCCAG CCAGCCATGC CATGCGTGTG 
TACGAGATGC TGATTAATCA TATCCAGCTT CACTACAAAT ACAATTACAG CTCACCCATT 
GCTACCAGTA TACGCCTCCA GGTTTTTGAC TTCCTCCTAC ACCTCCGAGC TGACTCTTTG 
CGGCGTGTTG GGCTACCTAA CAAAGATGGC GCCTTCAGAT TCAGTCCCTA TTGTCTTTGT 
GAATCTGTAA ATGAACAAGA GAAGAGAGCA GCTGAACGCA AGCCAGCAGG CACCTTGTCA 
CCCCCCTCTG GTAGCCCCAG TGTCCCATCA CAGACTGTCG CTCCACGCAT GGGATTCTTG 
CCATTCTCAC TAGCATTTGG GGTTATCCTG CAGTGCCTAA AGCAGGAAAC TGACTGGAAA 
GTCCTGAAAC TAGTCCTCAA CAAGCTGCCA GAGTGTCTGC GTTACAAACT GCTCATTCTG 
TCATCTCCCT GCAATATAGA TCAACTGGCT GGCTCTCTCT GTGCTATGCT TACAGACAAG 
ACTGCAAGTG ATCGCCTTTA CATTACTCCC GATGGCTTCT CTCGTACTGA CCTTCAGCTG 
GCAGTGGTTC CTGTACTGAC AGCCTTAACC TCATACCACC AACACCTAGA GCTCTCTAAA 
CAGAGGGAAA TGGTAAGGTG TCTGGAAACA GGCCTGATCT TTCGCTGCGG CAAGCAGTGC 
GTTGTGGCCC TTTCCATGTG TGTGATCGAG ATGCCAAGCA TTCTGATAAA ATCCCTCCCG 
GTCCTTATCG TGAAGCTAAC CCATATCTCG GCCACTGTCA CCATGGCCAT ACCAATGCTG 
GAGTTTCTTT CAACTTTGGT CCGCCTGCCC CACTTATATG CAAACTTTGC CGCAGAGCAG 
TATGCCAGTG TGTTTGCCAT CTCCCTTCCC TACACCAACC CATCAAAGTT TAATCAATAC 
ATTGTATTCC TTGCACACCA TGTCATTGCC ATGTGGTTCA TCCGGTGCAG ACTATCCCTA 
CGGAGAGACT TTGTGCCATA TATAACAAAG GGTTTGCGAT CCAATGTCCT GCTGAGCTTT 
GATGACACAC CGGAGAAGGG CAGCTTTAGA GCTCGCAGCA CCAGCTTGAA CGAGAGACCC 
AAAAGCTTGA AGCTATCTAA ACACACAAAG CCTGGCTTCA GTGACACGTC CCCAGTTAAG 
GAGATGAGAG ATCTCTCCAA TGCCTTCCGC TCTCGCAGCA TCAGCGTGTC GGAGCATGCT 
GCGTACAGCC GTATGCAGAC TTCTGTCACC AGCTCCAGCT TGAGTTCAGC TGATGAGAGC 
TCTATGGCCC AGGCTGATGA CAGCCTGAAA AACCTTCACC TGGAATTGAC CGAAATCTGC 
CTGGATATGA TGGCACGATA CGCCTTTGCC AACTTCACAG CCGTGCCCAA AAGGTCGCCT 
GTAGGTGAGT TCCTGCTGGA TGGCGGAAGA ACGAAGACCT GGCTAGTTGG GAACAAGCTG 
ATTACCATTA CAACAAGTGT GGGCATGGGG ACACGTTCTC TTCTTGGCCT GGATACAGGA 
GGGGAGAACC ATGGTGACAC TGAGAACAGC TCCCCCGTGC AAACAGAGCA AGTCAAGGAG 
TTTCCGTCCA TGCTCGATCT TCAGCAAAGA AAGCCGGGTA TGCAAACGCC CCGTCACAGA 
GTCCGCTCCA TGTCTGGAGG GACTACACTG AGAGCTGGAT CACTAGAGGG GACTCCTCTT 
TCGGCCAGTA TGGGGCAGCC GGGGGCCACT CCAGTGACCA GAGCAGAGAA ACCTTCCCGT 
AGGACACAGA TGAAGAAAGA AAAGACCACC CTGGCTGACT TTGTTCCCGT GCTGACACAA 
GGCTGGGCAG AGGTATTGGT TCGCAGACCC ACAGGTAACA CCAGCTGGTT GATGAGCCTG 
GAAAATCCTC TGAGCCCCTT CTCTTCTGAC ATAAACAACA TGCCGCTGCA GGAGCTTTCT 
AATGCCCTAA TGGCAGCCGA GAGGCTCAAG GAGCATCGGG AGACAGCGCT CTACAAATCT 
CTCTCTGTGC CAGCTTCAAC TTTATCACCC TCAACAACCA AGTGTACCCT TCTACAGCGG 
GCAAACACAG TGGCCTCTGT GTCTCTCGTA ACCCAGTCCA GTGGTCAAGG AAAGCTGCTT 
AGGAGCATTT CCTGGGCAGA ATCTTGTGCT GTGCCAGAGG AAGGGGCGCA AGCTGATGGG 
GAAGCAGAGG AAGTGTCCAC TCCCGGGGGC TCTCAAGAGC TGGAAGATTT TGAGGCTGTG 
GTTAGCGAAG ACACCACTAG TGAAGAGAAG AGTGCTAAGA AGATGGAGTG CATTAGCAGG 
TCTTCATCCA CATCCAGTCA GGAAGAGTCT GCTGCTACCC AAACGGAGGA ATCCGAGGCT 
GGTGGAATAC CCATTGAGGG CTCCACTCGG GAGGTCCCAT TTCAGACTTT GAGCAAGTCC 
AGCTCATCTC CTGAACTTCA GACCTTGCAG GAGCTTCCTA AAGAGTTGGG CCCTACAGAA 
GATATAAAGA AGCCTTCTGC AGACTTCAAA ACACTACCCC ATGTAGAAAA CCCTTTAAGC 
CACAGAACTA AATCTGAAGA GGAACGCAGT GCTGGGGCAT TAGCCAAACC AGAAGGCGAA 
ATGCGACTTG AACCAGAGTT GCCTGTTCTG TCCCCACAAT CTCCAACTGG GCACCGCCCA 
AGGGGTCACA CTATCTCTGA TTCTGAGCCG TCCCGCAGAG GCAGGAGATT TCAGGTTGAT 
CCTTTTAAGA ACAGCACCAA ATCCACCAAA GCTGAGAAAG TGCCTGGAAT AGATCCAAGC 
TTTGTATTTT TGCAGCTGTA CCATTCCCCA TTCTTTGGTG ATGAGTCCAA TAAGCCTATA 
CTGGTCCCTA ACACACAGAC GTTTGAGAGA ACAATGGGCC TCCTAGATCG AATCCCGCCC 
TATGACACCC ACAAAATTGG TGTTCTTTAT GTTGGAGAGG GGCAGGTTGG CAACGAGCGC 
GCAATCCTAT CCAATGAGCA TGGCTCGTAC CGCTACACAC AGTTCCTCAC GGGGCTTGGG 
AAGCTCATCG AGCTGAAAGA CACTCAGCCA GACAAGATCT TCCTGGGAGG GTTGGACGGT 
TGTGGAGATG ACGGGCAGTT CACCTACTGC TGGCATGATG ATATTATGCA AGTCATTTTT 
CAGATCACCA CCCTGATGCC AAACAAAGAG ATAGATCCGA ACCGCTGCAA CAAGAAAAGG 
CACGTGGGCA ACAACTTTGT CAATATCATC TACAACGACT CGGGCGAGTT CTACAAGTTG 
GGCACCCTCA AGGGTCAGTT CAACTTTGTA GATGTCAACA TTCAGCCTTT GGACTGCGAG 
AGCAACCTGG TGACACTGCA ATGCCGAAAA GATATGGAAG GCCTTGTAGA TACCAGCGTG 
GCAAAAATTG TGTCTGACAA AAACCTACCC TTCCTGGCCC GCCAGATGGC TCTACATTCA 
AATATGGCTT CACAAGTCCA TCACAGCAGG TCCAACCCCA CAGATATTTA CCCTTCTAAA 
TGGATTGCCA GGCTGCGACA CATAAAGAAG ATGAGACCAA AGATACAAGA GGAGTTGCAG 
GAGCTGAGTG CCAACATGCC CTACCAGGTC CCTACTATGG CCAACAAGCC CCCTTCACAA 
AACCCACAGA CTCCCCCCGG CGCATTTGAA ACCGGCCAAA GGACAAGGCT CATTTCTGCT 
GTGGACGATT TCACGGAGTT TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tsc2 ( XM_012970166.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825620.1 Xenopus tropicalis tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.

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CloneID	OXa32399
Clone ID Related Accession (Same CDS sequence)	XM_012970166.2
Accession Version	XM_012970166.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGGGTG AAAGACTTGG CGTCCTTCGT 
GCCCATTTCT TCCGAGTCAT AAAAGAGTAT CCTATGAATG AGGACCTTGC AGAGCGACTG 
GAGGTTTTTA AAGCTCTCAC CGACAATGGG CGAGAAATTG CTTACTTAGA AGAAGAGCTC 
GCTGTCTTCG TCCTCCAGTG GATGGAAATT GGTTTGACTC CGGATTTCCT GCATGTTTTA 
GTTAACCTGG TTAAGTTCAA TAGCTGTTAC TTGGAACACT TTATTGCTCC TATGGTGCAG 
ATGATATGCC TCCTCTGTAA TCGCACGACA TCTCCTTACG ATATTGAGGT TTCCTTGAAG 
GTTCTGGATG CAGTGGTCTG CTATAACTGC CTTCCATCAG AGAGCATTCC TATATTTGTT 
ATGACCCTGT GCCGCACAAT AAATGTTAAG GAGCTGTGTG AACCATGCTG GAAGCTGATG 
CGAAACCTTC TGGGCACCCA TCTTGGGCAC AGCGCCATTT ACACTATGAG TCGTATAATG 
GAGGATAGAG CCCACATGGA AGATGCCCCT TTGCTAAGAG GAGCTGTGTT CTTTGTTGGG 
ATGGGTCTCT GGGGAGCTCA CAGACTCTTC TGTCTGAAGA ACTCCCCGAT ATCTGTTCTC 
CCATCTTTCC TGAAGGCTAT GAACTGTAAC AATGCCGTTG TCTCCTATGA GATTGTCCTC 
TCTGTCACTC GGCTAATCAA GAAGTACGGC AAGGATGTGC AGGCTGCCAC GTGGGACGTT 
CTCCTTGATA TCATTGAGCG ATTATTCCAG CAGCTTCAGG TGCTGCGGAG TCAGGAATTA 
AAGGCTGCGG TGCACAATCT CATGACCACC ACAGAGGAGC TTTATAACCA GAACGGATTC 
CATGGAGATG AAGAGAGATT TTTCGAGCTG GTTGAAAAAG GGGCAGATGA AAGACCTTTT 
GCATCAGTTG TGAACCTTAT CACCTACAGA GCTCAGTCCA TTCACCCTGG GAAGGAAGGG 
TGGATTATGA ATCTACAGAA ACTGATGGAT AGATATTTCA GGAGCGAGTC TCGCAGTGTT 
GTACGCATGA AAATCTTAGA TGTTCTGTCC TTTGCGTTGA GCATCAATCG CCAGTTTTAC 
GAGGATGAGT TGATCGAGAA GGTAGTTACT TGCCAGCTAG CCCATATACC CGAGGATAAG 
GACCACCAGG TGCGCAAACT CGCAACACAG CTCCTTGTGG ACCTGGCTGA ATGCTGTCAC 
TCCTGCCACT TCAATAGTCT CATGGATATC ATTGAGAAGG TGGTGAACCG TTCCTTGTCC 
ACACCAGAGT CAGGTCATGA TTCTTCTTCT TTGGAAGATG TGAAAACCTC TGTTCTGGGG 
CTGATGGTTA TTCTACAGAC CAAGCTATAC ATTCTGCCAG CCAGCCATGC CATGCGTGTG 
TACGAGATGC TGATTAATCA TATCCAGCTT CACTACAAAT ACAATTACAG CTCACCCATT 
GCTACCAGTA TACGCCTCCA GGTTTTTGAC TTCCTCCTAC ACCTCCGAGC TGACTCTTTG 
CGGCGTGTTG GGCTACCTAA CAAAGATGGC GCCTTCAGAT TCAGTCCCTA TTGTCTTTGT 
GAATCTGTAA ATGAACAAGA GAAGAGAGCA GCTGAACGCA AGCCAGCAGG CACCTTGTCA 
CCCCCCTCTG GTAGCCCCAG TGTCCCATCA CAGACTGTCG CTCCACGCAT GGGATTCTTG 
CCATTCTCAC TAGCATTTGG GGTTATCCTG CAGTGCCTAA AGCAGGAAAC TGACTGGAAA 
GTCCTGAAAC TAGTCCTCAA CAAGCTGCCA GAGTGTCTGC GTTACAAACT GCTCATTCTG 
TCATCTCCCT GCAATATAGA TCAACTGGCT GGCTCTCTCT GTGCTATGCT TACAGACAAG 
ACTGCAAGTG ATCGCCTTTA CATTACTCCC GATGGCTTCT CTCGTACTGA CCTTCAGCTG 
GCAGTGGTTC CTGTACTGAC AGCCTTAACC TCATACCACC AACACCTAGA GCTCTCTAAA 
CAGAGGGAAA TGGTAAGGTG TCTGGAAACA GGCCTGATCT TTCGCTGCGG CAAGCAGTGC 
GTTGTGGCCC TTTCCATGTG TGTGATCGAG ATGCCAAGCA TTCTGATAAA ATCCCTCCCG 
GTCCTTATCG TGAAGCTAAC CCATATCTCG GCCACTGTCA CCATGGCCAT ACCAATGCTG 
GAGTTTCTTT CAACTTTGGT CCGCCTGCCC CACTTATATG CAAACTTTGC CGCAGAGCAG 
TATGCCAGTG TGTTTGCCAT CTCCCTTCCC TACACCAACC CATCAAAGTT TAATCAATAC 
ATTGTATTCC TTGCACACCA TGTCATTGCC ATGTGGTTCA TCCGGTGCAG ACTATCCCTA 
CGGAGAGACT TTGTGCCATA TATAACAAAG GGTTTGCGAT CCAATGTCCT GCTGAGCTTT 
GATGACACAC CGGAGAAGGG CAGCTTTAGA GCTCGCAGCA CCAGCTTGAA CGAGAGACCC 
AAAAGCCGTA TGCAGACTTC TGTCACCAGC TCCAGCTTGA GTTCAGCTGA TGAGAGCTCT 
ATGGCCCAGG CTGATGACAG CCTGAAAAAC CTTCACCTGG AATTGACCGA AATCTGCCTG 
GATATGATGG CACGATACGC CTTTGCCAAC TTCACAGCCG TGCCCAAAAG GTCGCCTGTA 
GGTGAGTTCC TGCTGGATGG CGGAAGAACG AAGACCTGGC TAGTTGGGAA CAAGCTGATT 
ACCATTACAA CAAGTGTGGG CATGGGGACA CGTTCTCTTC TTGGCCTGGA TACAGGAGGG 
GAGAACCATG GTGACACTGA GAACAGCTCC CCCGTGCAAA CAGAGCAAGT CAAGGAGTTT 
CCGTCCATGC TCGATCTTCA GCAAAGAAAG CCGGGTATGC AAACGCCCCG TCACAGAGTC 
CGCTCCATGT CTGGAGGGAC TACACTGAGA GCTGGATCAC TAGAGGGGAC TCCTCTTTCG 
GCCAGTATGG GGCAGCCGGG GGCCACTCCA GTGACCAGAG CAGAGAAACC TTCCCGTAGG 
ACACAGATGA AGAAAGAAAA GACCACCCTG GCTGACTTTG TTCCCGTGCT GACACAAGGC 
TGGGCAGAGG TATTGGTTCG CAGACCCACA GGTAACACCA GCTGGTTGAT GAGCCTGGAA 
AATCCTCTGA GCCCCTTCTC TTCTGACATA AACAACATGC CGCTGCAGGA GCTTTCTAAT 
GCCCTAATGG CAGCCGAGAG GCTCAAGGAG CATCGGGAGA CAGCGCTCTA CAAATCTCTC 
TCTGTGCCAG CTTCAACTTT ATCACCCTCA ACAACCAAGT GTACCCTTCT ACAGCGGGCA 
AACACAGTGG CCTCTGTGTC TCTCGTAACC CAGTCCAGTG GTCAAGGAAA GCTGCTTAGG 
AGCATTTCCT GGGCAGAATC TTGTGCTGTG CCAGAGGAAG GGGCGCAAGC TGATGGGGAA 
GCAGAGGAAG TGTCCACTCC CGGGGGCTCT CAAGAGCTGG AAGATTTTGA GGCTGTGGTT 
AGCGAAGACA CCACTAGTGA AGAGAAGAGT GCTAAGAAGA TGGAGTGCAT TAGCAGGTCT 
TCATCCACAT CCAGTCAGGA AGAGTCTGCT GCTACCCAAA CGGAGGAATC CGAGGCTGGT 
GGAATACCCA TTGAGGGCTC CACTCGGGAG GTCCCATTTC AGACTTTGAG CAAGTCCAGC 
TCATCTCCTG AACTTCAGAC CTTGCAGGAG CTTCCTAAAG AGTTGGGCCC TACAGAAGAT 
ATAAAGAAGC CTTCTGCAGA CTTCAAAACA CTACCCCATG TAGAAAACCC TTTAAGCCAC 
AGAACTAAAT CTGAAGAGGA ACGCAGTGCT GGGGCATTAG CCAAACCAGA AGGCGAAATG 
CGACTTGAAC CAGAGTTGCC TGTTCTGTCC CCACAATCTC CAACTGGGCA CCGCCCAAGG 
GGTCACACTA TCTCTGATTC TGAGCCGTCC CGCAGAGGCA GGAGATTTCA GGTTGATCCT 
TTTAAGAACA GCACCAAATC CACCAAAGCT GAGAAAGTGC CTGGAATAGA TCCAAGCTTT 
GTATTTTTGC AGCTGTACCA TTCCCCATTC TTTGGTGATG AGTCCAATAA GCCTATACTG 
GTCCCTAACA CACAGACGTT TGAGAGAACA ATGGGCCTCC TAGATCGAAT CCCGCCCTAT 
GACACCCACA AAATTGGTGT TCTTTATGTT GGAGAGGGGC AGGTTGGCAA CGAGCGCGCA 
ATCCTATCCA ATGAGCATGG CTCGTACCGC TACACACAGT TCCTCACGGG GCTTGGGAAG 
CTCATCGAGC TGAAAGACAC TCAGCCAGAC AAGATCTTCC TGGGAGGGTT GGACGGTTGT 
GGAGATGACG GGCAGTTCAC CTACTGCTGG CATGATGATA TTATGCAAGT CATTTTTCAG 
ATCACCACCC TGATGCCAAA CAAAGAGATA GATCCGAACC GCTGCAACAA GAAAAGGCAC 
GTGGGCAACA ACTTTGTCAA TATCATCTAC AACGACTCGG GCGAGTTCTA CAAGTTGGGC 
ACCCTCAAGG GTCAGTTCAA CTTTGTAGAT GTCAACATTC AGCCTTTGGA CTGCGAGAGC 
AACCTGGTGA CACTGCAATG CCGAAAAGAT ATGGAAGGCC TTGTAGATAC CAGCGTGGCA 
AAAATTGTGT CTGACAAAAA CCTACCCTTC CTGGCCCGCC AGATGGCTCT ACATTCAAAT 
ATGGCTTCAC AAGTCCATCA CAGCAGGTCC AACCCCACAG ATATTTACCC TTCTAAATGG 
ATTGCCAGGC TGCGACACAT AAAGAAGATG AGACCAAAGA TACAAGAGGA GTTGCAGGAG 
CTGAGTGCCA ACATGCCCTA CCAGGTCCCT ACTATGGCCA ACAAGCCCCC TTCACAAAAC 
CCACAGACTC CCCCCGGCGC ATTTGAAACC GGCCAAAGGA CAAGGCTCAT TTCTGCTGTG 
GACGATTTCA CGGAGTTTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825620.1
CDS	123..5426
Translation	MAKQMGKDPGLKEKVKMLLGLVPARVKTKPSEWKHPEYVITADLMKELSSESALHVRIRAIIQVCELAKTKKFEEHAVEAICQSVGDLIQPEQPVEARHHVLQLLKAIIVGQGERLGVLRAHFFRVIKEYPMNEDLAERLEVFKALTDNGREIAYLEEELAVFVLQWMEIGLTPDFLHVLVNLVKFNSCYLEHFIAPMVQMICLLCNRTTSPYDIEVSLKVLDAVVCYNCLPSESIPIFVMTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYTMSRIMEDRAHMEDAPLLRGAVFFVGMGLWGAHRLFCLKNSPISVLPSFLKAMNCNNAVVSYEIVLSVTRLIKKYGKDVQAATWDVLLDIIERLFQQLQVLRSQELKAAVHNLMTTTEELYNQNGFHGDEERFFELVEKGADERPFASVVNLITYRAQSIHPGKEGWIMNLQKLMDRYFRSESRSVVRMKILDVLSFALSINRQFYEDELIEKVVTCQLAHIPEDKDHQVRKLATQLLVDLAECCHSCHFNSLMDIIEKVVNRSLSTPESGHDSSSLEDVKTSVLGLMVILQTKLYILPASHAMRVYEMLINHIQLHYKYNYSSPIATSIRLQVFDFLLHLRADSLRRVGLPNKDGAFRFSPYCLCESVNEQEKRAAERKPAGTLSPPSGSPSVPSQTVAPRMGFLPFSLAFGVILQCLKQETDWKVLKLVLNKLPECLRYKLLILSSPCNIDQLAGSLCAMLTDKTASDRLYITPDGFSRTDLQLAVVPVLTALTSYHQHLELSKQREMVRCLETGLIFRCGKQCVVALSMCVIEMPSILIKSLPVLIVKLTHISATVTMAIPMLEFLSTLVRLPHLYANFAAEQYASVFAISLPYTNPSKFNQYIVFLAHHVIAMWFIRCRLSLRRDFVPYITKGLRSNVLLSFDDTPEKGSFRARSTSLNERPKSRMQTSVTSSSLSSADESSMAQADDSLKNLHLELTEICLDMMARYAFANFTAVPKRSPVGEFLLDGGRTKTWLVGNKLITITTSVGMGTRSLLGLDTGGENHGDTENSSPVQTEQVKEFPSMLDLQQRKPGMQTPRHRVRSMSGGTTLRAGSLEGTPLSASMGQPGATPVTRAEKPSRRTQMKKEKTTLADFVPVLTQGWAEVLVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERLKEHRETALYKSLSVPASTLSPSTTKCTLLQRANTVASVSLVTQSSGQGKLLRSISWAESCAVPEEGAQADGEAEEVSTPGGSQELEDFEAVVSEDTTSEEKSAKKMECISRSSSTSSQEESAATQTEESEAGGIPIEGSTREVPFQTLSKSSSSPELQTLQELPKELGPTEDIKKPSADFKTLPHVENPLSHRTKSEEERSAGALAKPEGEMRLEPELPVLSPQSPTGHRPRGHTISDSEPSRRGRRFQVDPFKNSTKSTKAEKVPGIDPSFVFLQLYHSPFFGDESNKPILVPNTQTFERTMGLLDRIPPYDTHKIGVLYVGEGQVGNERAILSNEHGSYRYTQFLTGLGKLIELKDTQPDKIFLGGLDGCGDDGQFTYCWHDDIMQVIFQITTLMPNKEIDPNRCNKKRHVGNNFVNIIYNDSGEFYKLGTLKGQFNFVDVNIQPLDCESNLVTLQCRKDMEGLVDTSVAKIVSDKNLPFLARQMALHSNMASQVHHSRSNPTDIYPSKWIARLRHIKKMRPKIQEELQELSANMPYQVPTMANKPPSQNPQTPPGAFETGQRTRLISAVDDFTEFA

Target ORF information:

RefSeq Version	XM_012970166.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tuberous sclerosis 2 (tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970166.2

ORF Insert Sequence:

ATGGCCAAGC AGATGGGTAA AGATCCTGGC CTGAAGGAAA AGGTGAAAAT GCTATTGGGA 
TTGGTGCCTG CACGAGTTAA AACCAAGCCT TCAGAGTGGA AACATCCGGA ATATGTCATT 
ACTGCAGATT TAATGAAGGA GCTGAGCTCT GAGTCTGCTC TCCACGTTCG CATCCGTGCA 
ATTATTCAGG TCTGTGAGCT TGCAAAGACG AAAAAATTTG AAGAGCATGC AGTAGAAGCA 
ATCTGCCAAT CAGTTGGTGA CTTGATACAG CCCGAACAGC CAGTAGAAGC TCGACATCAC 
GTTCTGCAGT TACTAAAGGC CATTATTGTG GGGCAGGGTG AAAGACTTGG CGTCCTTCGT 
GCCCATTTCT TCCGAGTCAT AAAAGAGTAT CCTATGAATG AGGACCTTGC AGAGCGACTG 
GAGGTTTTTA AAGCTCTCAC CGACAATGGG CGAGAAATTG CTTACTTAGA AGAAGAGCTC 
GCTGTCTTCG TCCTCCAGTG GATGGAAATT GGTTTGACTC CGGATTTCCT GCATGTTTTA 
GTTAACCTGG TTAAGTTCAA TAGCTGTTAC TTGGAACACT TTATTGCTCC TATGGTGCAG 
ATGATATGCC TCCTCTGTAA TCGCACGACA TCTCCTTACG ATATTGAGGT TTCCTTGAAG 
GTTCTGGATG CAGTGGTCTG CTATAACTGC CTTCCATCAG AGAGCATTCC TATATTTGTT 
ATGACCCTGT GCCGCACAAT AAATGTTAAG GAGCTGTGTG AACCATGCTG GAAGCTGATG 
CGAAACCTTC TGGGCACCCA TCTTGGGCAC AGCGCCATTT ACACTATGAG TCGTATAATG 
GAGGATAGAG CCCACATGGA AGATGCCCCT TTGCTAAGAG GAGCTGTGTT CTTTGTTGGG 
ATGGGTCTCT GGGGAGCTCA CAGACTCTTC TGTCTGAAGA ACTCCCCGAT ATCTGTTCTC 
CCATCTTTCC TGAAGGCTAT GAACTGTAAC AATGCCGTTG TCTCCTATGA GATTGTCCTC 
TCTGTCACTC GGCTAATCAA GAAGTACGGC AAGGATGTGC AGGCTGCCAC GTGGGACGTT 
CTCCTTGATA TCATTGAGCG ATTATTCCAG CAGCTTCAGG TGCTGCGGAG TCAGGAATTA 
AAGGCTGCGG TGCACAATCT CATGACCACC ACAGAGGAGC TTTATAACCA GAACGGATTC 
CATGGAGATG AAGAGAGATT TTTCGAGCTG GTTGAAAAAG GGGCAGATGA AAGACCTTTT 
GCATCAGTTG TGAACCTTAT CACCTACAGA GCTCAGTCCA TTCACCCTGG GAAGGAAGGG 
TGGATTATGA ATCTACAGAA ACTGATGGAT AGATATTTCA GGAGCGAGTC TCGCAGTGTT 
GTACGCATGA AAATCTTAGA TGTTCTGTCC TTTGCGTTGA GCATCAATCG CCAGTTTTAC 
GAGGATGAGT TGATCGAGAA GGTAGTTACT TGCCAGCTAG CCCATATACC CGAGGATAAG 
GACCACCAGG TGCGCAAACT CGCAACACAG CTCCTTGTGG ACCTGGCTGA ATGCTGTCAC 
TCCTGCCACT TCAATAGTCT CATGGATATC ATTGAGAAGG TGGTGAACCG TTCCTTGTCC 
ACACCAGAGT CAGGTCATGA TTCTTCTTCT TTGGAAGATG TGAAAACCTC TGTTCTGGGG 
CTGATGGTTA TTCTACAGAC CAAGCTATAC ATTCTGCCAG CCAGCCATGC CATGCGTGTG 
TACGAGATGC TGATTAATCA TATCCAGCTT CACTACAAAT ACAATTACAG CTCACCCATT 
GCTACCAGTA TACGCCTCCA GGTTTTTGAC TTCCTCCTAC ACCTCCGAGC TGACTCTTTG 
CGGCGTGTTG GGCTACCTAA CAAAGATGGC GCCTTCAGAT TCAGTCCCTA TTGTCTTTGT 
GAATCTGTAA ATGAACAAGA GAAGAGAGCA GCTGAACGCA AGCCAGCAGG CACCTTGTCA 
CCCCCCTCTG GTAGCCCCAG TGTCCCATCA CAGACTGTCG CTCCACGCAT GGGATTCTTG 
CCATTCTCAC TAGCATTTGG GGTTATCCTG CAGTGCCTAA AGCAGGAAAC TGACTGGAAA 
GTCCTGAAAC TAGTCCTCAA CAAGCTGCCA GAGTGTCTGC GTTACAAACT GCTCATTCTG 
TCATCTCCCT GCAATATAGA TCAACTGGCT GGCTCTCTCT GTGCTATGCT TACAGACAAG 
ACTGCAAGTG ATCGCCTTTA CATTACTCCC GATGGCTTCT CTCGTACTGA CCTTCAGCTG 
GCAGTGGTTC CTGTACTGAC AGCCTTAACC TCATACCACC AACACCTAGA GCTCTCTAAA 
CAGAGGGAAA TGGTAAGGTG TCTGGAAACA GGCCTGATCT TTCGCTGCGG CAAGCAGTGC 
GTTGTGGCCC TTTCCATGTG TGTGATCGAG ATGCCAAGCA TTCTGATAAA ATCCCTCCCG 
GTCCTTATCG TGAAGCTAAC CCATATCTCG GCCACTGTCA CCATGGCCAT ACCAATGCTG 
GAGTTTCTTT CAACTTTGGT CCGCCTGCCC CACTTATATG CAAACTTTGC CGCAGAGCAG 
TATGCCAGTG TGTTTGCCAT CTCCCTTCCC TACACCAACC CATCAAAGTT TAATCAATAC 
ATTGTATTCC TTGCACACCA TGTCATTGCC ATGTGGTTCA TCCGGTGCAG ACTATCCCTA 
CGGAGAGACT TTGTGCCATA TATAACAAAG GGTTTGCGAT CCAATGTCCT GCTGAGCTTT 
GATGACACAC CGGAGAAGGG CAGCTTTAGA GCTCGCAGCA CCAGCTTGAA CGAGAGACCC 
AAAAGCCGTA TGCAGACTTC TGTCACCAGC TCCAGCTTGA GTTCAGCTGA TGAGAGCTCT 
ATGGCCCAGG CTGATGACAG CCTGAAAAAC CTTCACCTGG AATTGACCGA AATCTGCCTG 
GATATGATGG CACGATACGC CTTTGCCAAC TTCACAGCCG TGCCCAAAAG GTCGCCTGTA 
GGTGAGTTCC TGCTGGATGG CGGAAGAACG AAGACCTGGC TAGTTGGGAA CAAGCTGATT 
ACCATTACAA CAAGTGTGGG CATGGGGACA CGTTCTCTTC TTGGCCTGGA TACAGGAGGG 
GAGAACCATG GTGACACTGA GAACAGCTCC CCCGTGCAAA CAGAGCAAGT CAAGGAGTTT 
CCGTCCATGC TCGATCTTCA GCAAAGAAAG CCGGGTATGC AAACGCCCCG TCACAGAGTC 
CGCTCCATGT CTGGAGGGAC TACACTGAGA GCTGGATCAC TAGAGGGGAC TCCTCTTTCG 
GCCAGTATGG GGCAGCCGGG GGCCACTCCA GTGACCAGAG CAGAGAAACC TTCCCGTAGG 
ACACAGATGA AGAAAGAAAA GACCACCCTG GCTGACTTTG TTCCCGTGCT GACACAAGGC 
TGGGCAGAGG TATTGGTTCG CAGACCCACA GGTAACACCA GCTGGTTGAT GAGCCTGGAA 
AATCCTCTGA GCCCCTTCTC TTCTGACATA AACAACATGC CGCTGCAGGA GCTTTCTAAT 
GCCCTAATGG CAGCCGAGAG GCTCAAGGAG CATCGGGAGA CAGCGCTCTA CAAATCTCTC 
TCTGTGCCAG CTTCAACTTT ATCACCCTCA ACAACCAAGT GTACCCTTCT ACAGCGGGCA 
AACACAGTGG CCTCTGTGTC TCTCGTAACC CAGTCCAGTG GTCAAGGAAA GCTGCTTAGG 
AGCATTTCCT GGGCAGAATC TTGTGCTGTG CCAGAGGAAG GGGCGCAAGC TGATGGGGAA 
GCAGAGGAAG TGTCCACTCC CGGGGGCTCT CAAGAGCTGG AAGATTTTGA GGCTGTGGTT 
AGCGAAGACA CCACTAGTGA AGAGAAGAGT GCTAAGAAGA TGGAGTGCAT TAGCAGGTCT 
TCATCCACAT CCAGTCAGGA AGAGTCTGCT GCTACCCAAA CGGAGGAATC CGAGGCTGGT 
GGAATACCCA TTGAGGGCTC CACTCGGGAG GTCCCATTTC AGACTTTGAG CAAGTCCAGC 
TCATCTCCTG AACTTCAGAC CTTGCAGGAG CTTCCTAAAG AGTTGGGCCC TACAGAAGAT 
ATAAAGAAGC CTTCTGCAGA CTTCAAAACA CTACCCCATG TAGAAAACCC TTTAAGCCAC 
AGAACTAAAT CTGAAGAGGA ACGCAGTGCT GGGGCATTAG CCAAACCAGA AGGCGAAATG 
CGACTTGAAC CAGAGTTGCC TGTTCTGTCC CCACAATCTC CAACTGGGCA CCGCCCAAGG 
GGTCACACTA TCTCTGATTC TGAGCCGTCC CGCAGAGGCA GGAGATTTCA GGTTGATCCT 
TTTAAGAACA GCACCAAATC CACCAAAGCT GAGAAAGTGC CTGGAATAGA TCCAAGCTTT 
GTATTTTTGC AGCTGTACCA TTCCCCATTC TTTGGTGATG AGTCCAATAA GCCTATACTG 
GTCCCTAACA CACAGACGTT TGAGAGAACA ATGGGCCTCC TAGATCGAAT CCCGCCCTAT 
GACACCCACA AAATTGGTGT TCTTTATGTT GGAGAGGGGC AGGTTGGCAA CGAGCGCGCA 
ATCCTATCCA ATGAGCATGG CTCGTACCGC TACACACAGT TCCTCACGGG GCTTGGGAAG 
CTCATCGAGC TGAAAGACAC TCAGCCAGAC AAGATCTTCC TGGGAGGGTT GGACGGTTGT 
GGAGATGACG GGCAGTTCAC CTACTGCTGG CATGATGATA TTATGCAAGT CATTTTTCAG 
ATCACCACCC TGATGCCAAA CAAAGAGATA GATCCGAACC GCTGCAACAA GAAAAGGCAC 
GTGGGCAACA ACTTTGTCAA TATCATCTAC AACGACTCGG GCGAGTTCTA CAAGTTGGGC 
ACCCTCAAGG GTCAGTTCAA CTTTGTAGAT GTCAACATTC AGCCTTTGGA CTGCGAGAGC 
AACCTGGTGA CACTGCAATG CCGAAAAGAT ATGGAAGGCC TTGTAGATAC CAGCGTGGCA 
AAAATTGTGT CTGACAAAAA CCTACCCTTC CTGGCCCGCC AGATGGCTCT ACATTCAAAT 
ATGGCTTCAC AAGTCCATCA CAGCAGGTCC AACCCCACAG ATATTTACCC TTCTAAATGG 
ATTGCCAGGC TGCGACACAT AAAGAAGATG AGACCAAAGA TACAAGAGGA GTTGCAGGAG 
CTGAGTGCCA ACATGCCCTA CCAGGTCCCT ACTATGGCCA ACAAGCCCCC TTCACAAAAC 
CCACAGACTC CCCCCGGCGC ATTTGAAACC GGCCAAAGGA CAAGGCTCAT TTCTGCTGTG 
GACGATTTCA CGGAGTTTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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tsc2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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