The following MYLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa38547 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015289794.1 | |
| Accession Version | XM_015289794.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | myosin light chain kinase, smooth muscle isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAATGA TTTCTGGTAT GAGTGGGAGG AAGGCCTCTG GGAGCTCGCC TACCAGCCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015145280.1 |
| CDS | 61..534 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_015289794.1 |
1 | ATGGCAATGA TTTCTGGTAT GAGTGGGAGG AAGGCCTCTG GGAGCTCGCC TACCAGCCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa86445 | |
| Clone ID Related Accession (Same CDS sequence) | NM_205459.3 | |
| Accession Version | NM_205459.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-04-08 | |
| Organism | Gallus gallus(chicken) | |
| Product | myosin light chain kinase, smooth muscle isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04023946.1. On Apr 12, 2016 this sequence version replaced NM_205459.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGGGGATG TTAAACTGGT GACATCTACT CGAGTATCCA AAACCTCCCT GACACTCAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_990790.2 |
| CDS | 113..5833 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_205459.3 |
1 | ATGGGGGATG TTAAACTGGT GACATCTACT CGAGTATCCA AAACCTCCCT GACACTCAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa86450 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001322361.1 | |
| Accession Version | NM_001322361.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-04-08 | |
| Organism | Gallus gallus(chicken) | |
| Product | myosin light chain kinase, smooth muscle isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04023946.1. On Apr 12, 2016 this sequence version replaced XM_015289793.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGCCGGGCT TGGAGCGTCG GAGGCGGGAG TTCCCCGCAG CTCTGTCGGA CCCGCTACCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001309290.1 |
| CDS | 83..5974 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001322361.1 |
1 | ATGCCGGGCT TGGAGCGTCG GAGGCGGGAG TTCCCCGCAG CTCTGTCGGA CCCGCTACCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates. |
Kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system. |
Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells. |
Characterization of tightly associated smooth muscle myosin-myosin light-chain kinase-calmodulin complexes. |
Two distinct modes of myosin assembly and dynamics during epithelial wound closure. |
