Mylk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mylk
Entrez Gene ID	288057
Full Name	myosin light chain kinase
General protein information	Preferred Names myosin light chain kinase Names myosin light chain kinase, smooth muscle myosin, light polypeptide kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597921.1	XP_017453410.1	myosin light chain kinase, smooth muscle isoform X1
XM_008768711.2	XP_008766933.1	myosin light chain kinase, smooth muscle isoform X2
XM_006248434.3	XP_006248496.1	myosin light chain kinase, smooth muscle isoform X1
XM_008768712.2	XP_008766934.1	myosin light chain kinase, smooth muscle isoform X3
NM_001105874.2	NP_001099344.2	myosin light chain kinase, smooth muscle
XM_006248433.3	XP_006248495.1	myosin light chain kinase, smooth muscle isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYLK	XP_005639631.1
	MYLK	XP_001113525.1
Gallus gallus (chicken)	MYLK	NP_990790.1
Xenopus tropicalis (tropical clawed frog)	LOC100489678	XP_002935598.2
Homo sapiens (human)	MYLK	NP_444253.3
Pan troglodytes (chimpanzee)	MYLK	XP_003950224.1
Mus musculus (house mouse)	Mylk	NP_647461.3
Rattus norvegicus (Norway rat)	Mylk	NP_001099344.2
Danio rerio (zebrafish)	mylkb	XP_002662355.2

Gene Ontology
Bibliography

Related articles in PubMed

Angiotensin II Facilitates Matrix Metalloproteinase-9-Mediated Myosin Light Chain Kinase Degradation in Pressure Overload-Induced Cardiac Hypertrophy.
Wang S, Cheng M, Hu Z, Hu S, Zou Q, Lai X, Liu B, Jiang H, Huang C, Wu G
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology44(6)2281-2295(2017)

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek M, Saito T, W?lfing C, Malissen B, Malissen M
Nature immunology14(8)858-66(2013 Aug)

Myosin light chain kinase is involved in the mechanism of gastrointestinal dysfunction in diabetic rats.
Hu W, Feng P
Digestive diseases and sciences57(5)1197-202(2012 May)

Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells.
Thatcher SE, Fultz ME, Tanaka H, Hagiwara H, Zhang HL, Zhang Y, Hayakawa K, Yoshiyama S, Nakamura A, Wang HH, Katayama T, Watanabe M, Lin Y, Wright GL, Kohama K
Journal of pharmacological sciences116(1)116-27(2011)

A family of protein-deglutamylating enzymes associated with neurodegeneration.
Rogowski K, van Dijk J, Magiera MM, Bosc C, Deloulme JC, Bosson A, Peris L, Gold ND, Lacroix B, Bosch Grau M, Bec N, Larroque C, Desagher S, Holzer M, Andrieux A, Moutin MJ, Janke C
Cell143(4)564-78(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression levels of Myosin light chain kinase in the pylorus and ileum of the diabetic rats were decreased and the changes of Myosin light chain kinase expression in these tissues were partially reversed after treatment with insulin.
Title: Myosin light chain kinase is involved in the mechanism of gastrointestinal dysfunction in diabetic rats.

In healthy young female rats, MLCK activity is higher and MLCP activity is lower in the bladder relative to the urethra
Title: Modulation of smooth muscle tonus in the lower urinary tract: interplay of myosin light-chain kinase (MLCK) and MLC phosphatase (MLCP).

Results from various lines of investigation suggest that the actin binding properties of MLCK play important roles in determining actin cytoskeletal organization in smooth muscle, particularly in cytoskeletal rearrangement stimulated by PDBu.
Title: Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells.

The following Mylk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mylk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa44661	XM_008768712.2 Latest version!	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa04367	NM_001105874.2 Latest version!	Rattus norvegicus myosin light chain kinase (Mylk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa34329	XM_017597921.1 Latest version!	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa34329	XM_006248434.3 Latest version!	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa34329	XM_006248433.3 Latest version!	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa44660	XM_008768711.2 Latest version!	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mylk ( XM_008768712.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766934.1 Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa44661
Clone ID Related Accession (Same CDS sequence)	XM_008768712.2
Accession Version	XM_008768712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin light chain kinase, smooth muscle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC GGAGACCCAA GAGCTCTCTT ACGCCTGTGC TAGGAACGGA AAGTGATGCT 
ACTGTGAAAA AGAAACCTGC CTCCAAGACA CCTACAAAAG CAGCTATGCC TCCTCAGATC 
ATCCAGTTCC CTGAGGACCA GAAGGTGCGT GCTGGAGAGC CAGTGGAGCT GTTTGGGAAA 
GTGACCGGTA CCCAGCCCAT CACCTGCACG TGGATGAAGT TCCGAAAGCA GATCCAGGAG 
AGCGAGAACA TCAGGGTGGA GAACAGTGAG AGCGGCAGCA AGCTCACCAT CCTGGCTGCA 
CGGCAGGAGC ACTGTGGCTG CTACACACTG GTGGTGGAGA ACAAGCTGAG CAGCAGACAG 
GCCCAAGTCA ACCTCACGGT TGTGGATAAG CCAGACCCCC CGGCTGGCAC ACCTTGTGCT 
TCTGACATAC GGAGTTCCTC GCTCACCCTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC 
AGTGCCGTAC AGTCCTACAA TGTTGAGATC TGGGACACGG AGGACAAGGT GTGGACAGAG 
CTAGCCACGT GCCGCAGCAC CTCTTTCAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAT 
AAATTCCGAG TACGTGCAGT CAACGTCTAC GGAACCAGCG AGCCGAGCCA GGAGTCTGAG 
CTCACCGCAG TGGGAGAGAA GCCTGAAGAG CCAAAGGATG AAGTGGAAGT GTCCGATGAT 
GACGAAAAGG AGCCTGAGGT TGATTACCGG ACGGTGACGG TGAACACTGA ACAGAAAGTA 
TCTGATGTAT ATGACATTGA AGAGCGACTA GGATCTGGGA AATTTGGACA AGTCTTCCGA 
CTTGTTGAAA AGAGAACTGG AAAAATCTGG GCAGGGAAAT TTTTCAAGGC CTATTCCGCC 
AAGGAGAAAG AAAACATCCG TCAGGAGATC AGCATCATGA ACTGTCTCCA CCACCCCAAG 
CTGGTCCAGT GCGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT TCTGGAGATT 
GTGTCAGGGG GTGAGCTATT TGAGCGCATC ATTGATGAGG ACTTCGAGCT AACAGAGCGG 
GAGTGCATCA AGTACATGAG GCAGATTTCG GAAGGGGTGG AGTACATCCA CAAGCAGGGC 
ATCGTGCATC TGGACCTCAA GCCTGAGAAC ATTATGTGTG TCAACAAGAC AGGCACCAGG 
ATCAAGCTCA TTGACTTCGG CCTGGCACGA AGACTCGAGA ACGCTGGGTC TCTGAAGGTC 
CTCTTTGGGA CCCCAGAGTT TGTGGCTCCT GAGGTGATCA ACTATGAGCC CATCGGCTAC 
GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTATATTC TGGTCAGCGG ACTTTCCCCC 
TTCATGGGTG ACAATGATAA CGAAACTTTA GCCAATGTCA CTTCAGCTAC CTGGGACTTC 
GACGACGAGG CGTTCGATGA GATCTCGGAA GATGCGAAGG ACTTCATCAG TAACTTGCTA 
AAGAAAGATA TGAAAAACCG TCTGGACTGC ACCCAGTGCC TTCAGCACCC ATGGCTGATG 
AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGGATGAA GAAATACATG 
GCAAGAAGGA AGTGGCAGAA AACAGGCAAT GCTGTGAGAG CCATTGGGAG ACTGTCCTCT 
ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCTTCAA CAGGGTCACC AACCAGCCCG 
ATCAATGCAG AGAAGCTAGA GTCTGAAGAA GATGTCTCCC AGGCCTTCCT CGAGGCTGTT 
GCTGAGGAAA AGCCCCATGT AAAGCCCTAC TTCTCTAAGA CCATTCGTGA CCTGGAGGTT 
GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATCGAAG GATACCCAGA CCCTGAGGTT 
GTCTGGTTCA AGGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT 
GAAGACGGGA ACTGCTCGCT AATTATCAGT GATGTCTGTG GGGATGATGA CGCCAAGTAC 
ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CCGCGGAGCT CATCGTGGAG 
ACCATGGAGG AAGGGGAAGG GGAGGAGGGG GGGGAGGAGG AGGAGGAGGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766934.1
CDS	489..2645
Translation	MKSRRPKSSLTPVLGTESDATVKKKPASKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESENIRVENSESGSKLTILAARQEHCGCYTLVVENKLSSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWTELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKRTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISEDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEE

Target ORF information:

RefSeq Version	XM_008768712.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768712.2

ORF Insert Sequence:

ATGAAATCCC GGAGACCCAA GAGCTCTCTT ACGCCTGTGC TAGGAACGGA AAGTGATGCT 
ACTGTGAAAA AGAAACCTGC CTCCAAGACA CCTACAAAAG CAGCTATGCC TCCTCAGATC 
ATCCAGTTCC CTGAGGACCA GAAGGTGCGT GCTGGAGAGC CAGTGGAGCT GTTTGGGAAA 
GTGACCGGTA CCCAGCCCAT CACCTGCACG TGGATGAAGT TCCGAAAGCA GATCCAGGAG 
AGCGAGAACA TCAGGGTGGA GAACAGTGAG AGCGGCAGCA AGCTCACCAT CCTGGCTGCA 
CGGCAGGAGC ACTGTGGCTG CTACACACTG GTGGTGGAGA ACAAGCTGAG CAGCAGACAG 
GCCCAAGTCA ACCTCACGGT TGTGGATAAG CCAGACCCCC CGGCTGGCAC ACCTTGTGCT 
TCTGACATAC GGAGTTCCTC GCTCACCCTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC 
AGTGCCGTAC AGTCCTACAA TGTTGAGATC TGGGACACGG AGGACAAGGT GTGGACAGAG 
CTAGCCACGT GCCGCAGCAC CTCTTTCAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAT 
AAATTCCGAG TACGTGCAGT CAACGTCTAC GGAACCAGCG AGCCGAGCCA GGAGTCTGAG 
CTCACCGCAG TGGGAGAGAA GCCTGAAGAG CCAAAGGATG AAGTGGAAGT GTCCGATGAT 
GACGAAAAGG AGCCTGAGGT TGATTACCGG ACGGTGACGG TGAACACTGA ACAGAAAGTA 
TCTGATGTAT ATGACATTGA AGAGCGACTA GGATCTGGGA AATTTGGACA AGTCTTCCGA 
CTTGTTGAAA AGAGAACTGG AAAAATCTGG GCAGGGAAAT TTTTCAAGGC CTATTCCGCC 
AAGGAGAAAG AAAACATCCG TCAGGAGATC AGCATCATGA ACTGTCTCCA CCACCCCAAG 
CTGGTCCAGT GCGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT TCTGGAGATT 
GTGTCAGGGG GTGAGCTATT TGAGCGCATC ATTGATGAGG ACTTCGAGCT AACAGAGCGG 
GAGTGCATCA AGTACATGAG GCAGATTTCG GAAGGGGTGG AGTACATCCA CAAGCAGGGC 
ATCGTGCATC TGGACCTCAA GCCTGAGAAC ATTATGTGTG TCAACAAGAC AGGCACCAGG 
ATCAAGCTCA TTGACTTCGG CCTGGCACGA AGACTCGAGA ACGCTGGGTC TCTGAAGGTC 
CTCTTTGGGA CCCCAGAGTT TGTGGCTCCT GAGGTGATCA ACTATGAGCC CATCGGCTAC 
GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTATATTC TGGTCAGCGG ACTTTCCCCC 
TTCATGGGTG ACAATGATAA CGAAACTTTA GCCAATGTCA CTTCAGCTAC CTGGGACTTC 
GACGACGAGG CGTTCGATGA GATCTCGGAA GATGCGAAGG ACTTCATCAG TAACTTGCTA 
AAGAAAGATA TGAAAAACCG TCTGGACTGC ACCCAGTGCC TTCAGCACCC ATGGCTGATG 
AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGGATGAA GAAATACATG 
GCAAGAAGGA AGTGGCAGAA AACAGGCAAT GCTGTGAGAG CCATTGGGAG ACTGTCCTCT 
ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCTTCAA CAGGGTCACC AACCAGCCCG 
ATCAATGCAG AGAAGCTAGA GTCTGAAGAA GATGTCTCCC AGGCCTTCCT CGAGGCTGTT 
GCTGAGGAAA AGCCCCATGT AAAGCCCTAC TTCTCTAAGA CCATTCGTGA CCTGGAGGTT 
GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATCGAAG GATACCCAGA CCCTGAGGTT 
GTCTGGTTCA AGGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT 
GAAGACGGGA ACTGCTCGCT AATTATCAGT GATGTCTGTG GGGATGATGA CGCCAAGTAC 
ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CCGCGGAGCT CATCGTGGAG 
ACCATGGAGG AAGGGGAAGG GGAGGAGGGG GGGGAGGAGG AGGAGGAGGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( NM_001105874.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099344.2 Rattus norvegicus myosin light chain kinase (Mylk), mRNA.

Price & Availability↑

CloneID	ORa04367
Clone ID Related Accession (Same CDS sequence)	NM_001105874.2
Accession Version	NM_001105874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	myosin light chain kinase, smooth muscle
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07034409.1 and AABR07034408.1. On Jul 9, 2010 this sequence version replaced NM_001105874.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because insufficient rat transcript evidence was available to represent the gene. The genomic coordinates used for the transcript record were based on alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCTGGCC AGTTACCTGG GAAGACCATG GGGGACGTGA AACTGTTTGC CTCGTCGCAC 
ATGTCCAAAA CCTCCCACAG TGTGGATCCC TCGAGGGTCA GCTCTCTGCC TCTCACCGAG 
GCCCCTGCTT TCATTCTGCC TCCTCGGAAC CTCTGCGTCA AAGAAGGAGC CACCGCCAAG 
TTTGAAGGGA GGGTCCGGGG CCACCCAGAG CCCCAGGTGA CATGGCACAG AAATGGGCAA 
ACCATCACCA ATGGCGGCCG CTTCCTGCTG GACGGTGGGG TGCGGGGGAC TTTCAGCCTC 
GTCATCCGTG GTGTCCGTGA GGAGGACAAG GGCAAGTACA CCTGTGAAGC TAGCAACGGC 
AGTGGCGCCC GCCAGGTGAC CGTGGAGCTG ACTGTGGAAG GGGATCTTAT GAAGAAGCAT 
GGTCAGCCCG TTCTTTCTAA AGCCTCAGGA TTTCCAGGGG AGACTCGTCC TAGCATCTGG 
GGTGAGTGCC CGCCAAAGTT TGCCACCAAG CTGGGCAGAG CTGTGGTCAA AGAAGGGCAG 
ATGGGGCGAT TTTCCTGCAA GATCACTGGC CGGCCTCCAC CACAGGTCAC CTGGCTCAAG 
GGAAATGTCC CGTTGCAGCC AAGTGCCCGA GTGTCTATGT CAGAGAAGAA TGGCATGCAG 
ATCCTGGAAA TCCACGAGGT CACTCAGGAC GACATGGGCA CGTACACATG CATGGTGGTG 
AATGGGTCAG GGAAAGCCTC CATGTCAGCG GAGCTCTCCA TCCCAGGTTT GGACAATGCC 
ACCAGGTTGT CAAGAGGAAC CAAGGACCCC AGTCCAGACA TCAGGAAGGA GGTGACCAAT 
GGAATCTCCA AGGACCCAGA GACTGTGGCT GAAAGTAAGA ACTGTTCAAG TCCCCAGAAG 
AGCAACTTCT TAGCCCAGGC CACCAACAGT CACCTCAAGT CCCTGCAGCA GCCAAAGCTG 
AAGCCGTGTG AAGATTCCCC CAGAAAGGTC CTGCAGTCCT CCGTCCTGCA GAAGACCTCC 
AGCACCATTG TTCTGCAGGC CTCAAAAGTC CAGCCAGAAG TGAGAGCAGC GGGCATAGGG 
GCCTCCGGTC CTGGGGAAGA GAGGAAGAGT CTGACTGCCC CTCGGCAAGC CACACCCCCT 
ACCAGACAGT GTGGCCTGGG AGGCTCAGTT GGGAACAAAT TTATCACGGG GAACATCCCC 
ATAGAGAACC AAAGGGAGTC AAGATTTCCC AAGTTTGAGA GTCAGCCCCA AAGCCAAGAA 
GTCACTGAAG GTCAAACAGT CAAGTTCACC TGTGAGGTTT CAGGGATACC AAAGCCTGAA 
GTGGGTTGGT TCCTGGAAGG CATCCCCGTG AGGAGACAAG AAGGCATCGT TGAGATTTAT 
GAAGACGGTA CATCCCATTA CCTGTGTCTG CCGAGAGCCG GGACGAGAGA CAGCGGGAGA 
TACAGCTGCA CAGCATCCAA TACCCTTGGC CAAGTGTCCT GCAGTTGGAC GCTCCGGGTG 
GACCGGCCAA GCTTGACGCA GACAGCCCCT TCCTTCTCTA GTGTTCTGAA GGACAGTGTT 
GTCATTGAAG GCCAGGATTT TGTGCTACGG TGCTCTGTAC GGGGGACCCC AGCACCCCAG 
GTCACTTGGC TGCTCAATGG GCAGCCCATC CAGTTTGCTC GATCCATCTG TGAGGCTGGC 
GAGGCCGAGC TCCACATCCA GGATGCCCTG CCCGAGGACC GTGGCACCTA CACCTGCCTG 
GCTGAGAATG CCTTGGGGCA GGTGTCCTGC AGTGCCACGG TCACGGTCCA AGAAAAGAAA 
GGCGAAGGAG AGAGGGCGCA CCGTCTGCCC CCAGCTCCCA GCAAGCTCTT CGCTCCCATC 
TTCCTGCAGG GTCTCTCTGA CCTCAAAGTC ATGGATGGAA GCCAGGTCAC TATGACAGTC 
CAGGTGTCAG GAAACCCACC TCCAGAAGTC ATATGGCTTC ACGACGGGAA CGAGATCCAG 
GAGTCAGAGG ACTTCCACTT CGAGCAGAAA GGTGGCTGGC ACAGCCTATG TATCCAGGAA 
GTGTTCCCGG AGGACACGGG CACATACACC TGTGAGGCCT GGAACAGCGC TGGGGAGGTC 
CGCACCCAGG CCATACTCAC CGTGCAAGAG CCCCATGATG GCACCCAGCC CTGGTTCATC 
AGCAAGCCTC GTTCGGTGAC AGCCTCCCTG GGCCAGAGTG TCCTCATCTC CTGCGCCATA 
GCTGGAGACC CCTTTCCCAC TGTGCACTGG CTCCGAGATG GCAAAGCCCT CTCCAGGGAC 
CATGGCCACT TTGAGCTGCT CCAGAATGAG GACGTGTTCA CCCTGGTTCT AAAGAACGTG 
CAGCCCTGGC ATGCCGGCCA GTATGAGATC CTGCTCAAGA ACCGGGTTGG CGAGTGCAGT 
TGCCAAGTGT CACTGATGCT GCACAAAGGT CCTTCCAGAG TCCCACCACC ACGGGGGCGG 
GAGCCTGCCA GCTGTGAGGG CCTCTGTGGT GGAGGAGTTG GTGCTCATGG TGATGCTGAC 
CGTGATGGGA CCCTGAGGCC CTGCTGGCCA GCAAGAGGGC AGGGTTGGCC TGAGGAGGAA 
GACGGAGAAG ACGTGAGGGG GCTGTTGAAG AGACGGGTGG AGACCAGGCT GCACACAGAA 
GAGGCCATCC GCCAGCAGGA GGTGGGGCAG CTGGATTTTC GAGACCTCCT GGGAAAGAAG 
GTGAGCACAA AGACCGTATC AGAAGACGAC CTGAAGGACA TCCCAGCCGA GCAGATGGAT 
TTCCGCGCCA ACCTTCAGCG GCAAGTGAAG CCAAAGACTG TGTCTGAGGA AGAGAGGAAG 
GTGCATAGCC CCCAGCAGGT TGACTTCCGG TCTGTCCTAG CCAAGAAGGG GACCCCTAAG 
ACCCCTGTCC CTGAGAAGGT GCCACCTCAA AAAGCTGCCA CCCCAGACTT TCGCTCAGTG 
CTAGGTAGCA AGAAGAAGTC GCCTGCGGAG AATGGGGGCA ACACTGCTGA GGTCTTGAAT 
GTCAAGGCTG GGGAAAGCCC CACGCCTGCA GGCAATGCAC AGCCAGTCGG GGCCCTGAAA 
CCCATAGGCA ACGCCAAGCC TGCTGAGACC CTGAAGTCCG TGGGCAATGC CAAACCAGCA 
GAGACCCTGA AGCCCGTGGG GAATGCCAAG CCAGCAGAGA CCCTGAAACC CGTAGGTAAT 
GTCAAGCCTG CCGAGACCCT GAAGCCCATA GCCAACGCAC AGCCTTCAGG GTCCCCAAAA 
CCCATAGCCA ACGCACAGCC TATTGAGACC CAGAAGTCTG TGGCCAATGC CAAGTCTGCC 
GAGACCCCGA AACTAGCGGG GAAAGAAGAA CTCAAGGAGG TTAGGAATGA TGTGAACTGT 
AAGAAAGCGC ATGTCGGAGC CACCGGCAAT GAAAAAAGAC CTGAGAGCCA AGGCTCGGCC 
CCAGTTTTCA AGGAAAAGCT ACAAGATGTC CATGTGGCTG AGGGCGAGAA GCTGCTACTC 
CAGTGCCAGG TGACCTCTGA TCCTCCGGCC ACTGTCACCT GGACACTGAA TGGAAAGACA 
CTCAAGACCA CAAAATTCAT CATCCTCGCC CAAGAAGGCT CACTCTTCTC CGTCTCCATC 
GAGAAGGCAC TGCCTGAGGA CAGGGGTCTA TACAAGTGTG TAGCCAAGAA CAGCGCTGGC 
CAGGCGGAAT GTTCCTGCCA AGTCACTGTG GACGATGCTG GGACCAGCGA GAACACCAAG 
GCCCCGGAGA TGAAATCCCG GAGACCCAAG AGCTCTCTTA CGCCTGTGCT AGGAACGGAA 
AGTGATGCTA CTGTGAAAAA GAAACCTGCC TCCAAGACAC CTACAAAAGC AGCTATGCCT 
CCTCAGATCA TCCAGTTCCC TGAGGACCAG AAGGTGCGTG CTGGAGAGCC AGTGGAGCTG 
TTTGGGAAAG TGACCGGTAC CCAGCCCATC ACCTGCACGT GGATGAAGTT CCGAAAGCAG 
ATCCAGGAGA GCGAGAACAT CAGGGTGGAG AACAGTGAGA GCGGCAGCAA GCTCACCATC 
CTGGCTGCAC GGCAGGAGCA CTGTGGCTGC TACACACTGG TGGTGGAGAA CAAGCTGAGC 
AGCAGACAGG CCCAAGTCAA CCTCACGGTT GTGGATAAGC CAGACCCCCC GGCTGGCACA 
CCTTGTGCTT CTGACATACG GAGTTCCTCG CTCACCCTGT CCTGGTATGG CTCCTCGTAT 
GATGGGGGCA GTGCCGTACA GTCCTACAAT GTTGAGATCT GGGACACGGA GGACAAGGTG 
TGGACAGAGC TAGCCACGTG CCGCAGCACC TCTTTCAATG TCCAAGACCT GCTGCCTGAC 
CGCGAGTATA AATTCCGAGT ACGTGCAGTC AACGTCTACG GAACCAGCGA GCCGAGCCAG 
GAGTCTGAGC TCACCGCAGT GGGAGAGAAG CCTGAAGAGC CAAAGGATGA AGTGGAAGTG 
TCCGATGATG ACGAAAAGGA GCCTGAGGTT GATTACCGGA CGGTGACGGT GAACACTGAA 
CAGAAAGTAT CTGATGTATA TGACATTGAA GAGCGACTAG GATCTGGGAA ATTTGGACAA 
GTCTTCCGAC TTGTTGAAAA GAGAACTGGA AAAATCTGGG CAGGGAAATT TTTCAAGGCC 
TATTCCGCCA AGGAGAAAGA AAACATCCGT CAGGAGATCA GCATCATGAA CTGTCTCCAC 
CACCCCAAGC TGGTCCAGTG CGTGGATGCC TTCGAAGAAA AGGCCAACAT CGTCATGGTT 
CTGGAGATTG TGTCAGGGGG TGAGCTATTT GAGCGCATCA TTGATGAGGA CTTCGAGCTA 
ACAGAGCGGG AGTGCATCAA GTACATGAGG CAGATTTCGG AAGGGGTGGA GTACATCCAC 
AAGCAGGGCA TCGTGCATCT GGACCTCAAG CCTGAGAACA TTATGTGTGT CAACAAGACA 
GGCACCAGGA TCAAGCTCAT TGACTTCGGC CTGGCACGAA GACTCGAGAA CGCTGGGTCT 
CTGAAGGTCC TCTTTGGGAC CCCAGAGTTT GTGGCTCCTG AGGTGATCAA CTATGAGCCC 
ATCGGCTACG CCACAGACAT GTGGAGCATC GGGGTCATCT GCTATATTCT GGTCAGCGGA 
CTTTCCCCCT TCATGGGTGA CAATGATAAC GAAACTTTAG CCAATGTCAC TTCAGCTACC 
TGGGACTTCG ACGACGAGGC GTTCGATGAG ATCTCGGAAG ATGCGAAGGA CTTCATCAGT 
AACTTGCTAA AGAAAGATAT GAAAAACCGT CTGGACTGCA CCCAGTGCCT TCAGCACCCA 
TGGCTGATGA AAGACACCAA GAACATGGAG GCCAAGAAGC TGTCCAAGGA CCGGATGAAG 
AAATACATGG CAAGAAGGAA GTGGCAGAAA ACAGGCAATG CTGTGAGAGC CATTGGGAGA 
CTGTCCTCTA TGGCAATGAT CTCAGGGCTC AGTGGCAGGA AATCTTCAAC AGGGTCACCA 
ACCAGCCCGA TCAATGCAGA GAAGCTAGAG TCTGAAGAAG ATGTCTCCCA GGCCTTCCTC 
GAGGCTGTTG CTGAGGAAAA GCCCCATGTA AAGCCCTACT TCTCTAAGAC CATTCGTGAC 
CTGGAGGTTG TGGAGGGAAG CGCTGCTAGA TTTGACTGCA AGATCGAAGG ATACCCAGAC 
CCTGAGGTTG TCTGGTTCAA GGATGACCAG TCAATCAGGG AGTCCCGCCA CTTCCAGATA 
GACTACGATG AAGACGGGAA CTGCTCGCTA ATTATCAGTG ATGTCTGTGG GGATGATGAC 
GCCAAGTACA CCTGCAAGGC TGTCAACAGC CTCGGAGAAG CCACCTGCAC CGCGGAGCTC 
ATCGTGGAGA CCATGGAGGA AGGGGAAGGG GAGGAGGGGG GGGAGGAGGA GGAGGAGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099344.2
CDS	41..5926
Translation	MPGQLPGKTMGDVKLFASSHMSKTSHSVDPSRVSSLPLTEAPAFILPPRNLCVKEGATAKFEGRVRGHPEPQVTWHRNGQTITNGGRFLLDGGVRGTFSLVIRGVREEDKGKYTCEASNGSGARQVTVELTVEGDLMKKHGQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRLSRGTKDPSPDIRKEVTNGISKDPETVAESKNCSSPQKSNFLAQATNSHLKSLQQPKLKPCEDSPRKVLQSSVLQKTSSTIVLQASKVQPEVRAAGIGASGPGEERKSLTAPRQATPPTRQCGLGGSVGNKFITGNIPIENQRESRFPKFESQPQSQEVTEGQTVKFTCEVSGIPKPEVGWFLEGIPVRRQEGIVEIYEDGTSHYLCLPRAGTRDSGRYSCTASNTLGQVSCSWTLRVDRPSLTQTAPSFSSVLKDSVVIEGQDFVLRCSVRGTPAPQVTWLLNGQPIQFARSICEAGEAELHIQDALPEDRGTYTCLAENALGQVSCSATVTVQEKKGEGERAHRLPPAPSKLFAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKALSRDHGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHKGPSRVPPPRGREPASCEGLCGGGVGAHGDADRDGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKVPPQKAATPDFRSVLGSKKKSPAENGGNTAEVLNVKAGESPTPAGNAQPVGALKPIGNAKPAETLKSVGNAKPAETLKPVGNAKPAETLKPVGNVKPAETLKPIANAQPSGSPKPIANAQPIETQKSVANAKSAETPKLAGKEELKEVRNDVNCKKAHVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILAQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAGTSENTKAPEMKSRRPKSSLTPVLGTESDATVKKKPASKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESENIRVENSESGSKLTILAARQEHCGCYTLVVENKLSSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWTELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKRTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISEDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEE

Target ORF information:

RefSeq Version	NM_001105874.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain kinase (Mylk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105874.2

ORF Insert Sequence:

ATGCCTGGCC AGTTACCTGG GAAGACCATG GGGGACGTGA AACTGTTTGC CTCGTCGCAC 
ATGTCCAAAA CCTCCCACAG TGTGGATCCC TCGAGGGTCA GCTCTCTGCC TCTCACCGAG 
GCCCCTGCTT TCATTCTGCC TCCTCGGAAC CTCTGCGTCA AAGAAGGAGC CACCGCCAAG 
TTTGAAGGGA GGGTCCGGGG CCACCCAGAG CCCCAGGTGA CATGGCACAG AAATGGGCAA 
ACCATCACCA ATGGCGGCCG CTTCCTGCTG GACGGTGGGG TGCGGGGGAC TTTCAGCCTC 
GTCATCCGTG GTGTCCGTGA GGAGGACAAG GGCAAGTACA CCTGTGAAGC TAGCAACGGC 
AGTGGCGCCC GCCAGGTGAC CGTGGAGCTG ACTGTGGAAG GGGATCTTAT GAAGAAGCAT 
GGTCAGCCCG TTCTTTCTAA AGCCTCAGGA TTTCCAGGGG AGACTCGTCC TAGCATCTGG 
GGTGAGTGCC CGCCAAAGTT TGCCACCAAG CTGGGCAGAG CTGTGGTCAA AGAAGGGCAG 
ATGGGGCGAT TTTCCTGCAA GATCACTGGC CGGCCTCCAC CACAGGTCAC CTGGCTCAAG 
GGAAATGTCC CGTTGCAGCC AAGTGCCCGA GTGTCTATGT CAGAGAAGAA TGGCATGCAG 
ATCCTGGAAA TCCACGAGGT CACTCAGGAC GACATGGGCA CGTACACATG CATGGTGGTG 
AATGGGTCAG GGAAAGCCTC CATGTCAGCG GAGCTCTCCA TCCCAGGTTT GGACAATGCC 
ACCAGGTTGT CAAGAGGAAC CAAGGACCCC AGTCCAGACA TCAGGAAGGA GGTGACCAAT 
GGAATCTCCA AGGACCCAGA GACTGTGGCT GAAAGTAAGA ACTGTTCAAG TCCCCAGAAG 
AGCAACTTCT TAGCCCAGGC CACCAACAGT CACCTCAAGT CCCTGCAGCA GCCAAAGCTG 
AAGCCGTGTG AAGATTCCCC CAGAAAGGTC CTGCAGTCCT CCGTCCTGCA GAAGACCTCC 
AGCACCATTG TTCTGCAGGC CTCAAAAGTC CAGCCAGAAG TGAGAGCAGC GGGCATAGGG 
GCCTCCGGTC CTGGGGAAGA GAGGAAGAGT CTGACTGCCC CTCGGCAAGC CACACCCCCT 
ACCAGACAGT GTGGCCTGGG AGGCTCAGTT GGGAACAAAT TTATCACGGG GAACATCCCC 
ATAGAGAACC AAAGGGAGTC AAGATTTCCC AAGTTTGAGA GTCAGCCCCA AAGCCAAGAA 
GTCACTGAAG GTCAAACAGT CAAGTTCACC TGTGAGGTTT CAGGGATACC AAAGCCTGAA 
GTGGGTTGGT TCCTGGAAGG CATCCCCGTG AGGAGACAAG AAGGCATCGT TGAGATTTAT 
GAAGACGGTA CATCCCATTA CCTGTGTCTG CCGAGAGCCG GGACGAGAGA CAGCGGGAGA 
TACAGCTGCA CAGCATCCAA TACCCTTGGC CAAGTGTCCT GCAGTTGGAC GCTCCGGGTG 
GACCGGCCAA GCTTGACGCA GACAGCCCCT TCCTTCTCTA GTGTTCTGAA GGACAGTGTT 
GTCATTGAAG GCCAGGATTT TGTGCTACGG TGCTCTGTAC GGGGGACCCC AGCACCCCAG 
GTCACTTGGC TGCTCAATGG GCAGCCCATC CAGTTTGCTC GATCCATCTG TGAGGCTGGC 
GAGGCCGAGC TCCACATCCA GGATGCCCTG CCCGAGGACC GTGGCACCTA CACCTGCCTG 
GCTGAGAATG CCTTGGGGCA GGTGTCCTGC AGTGCCACGG TCACGGTCCA AGAAAAGAAA 
GGCGAAGGAG AGAGGGCGCA CCGTCTGCCC CCAGCTCCCA GCAAGCTCTT CGCTCCCATC 
TTCCTGCAGG GTCTCTCTGA CCTCAAAGTC ATGGATGGAA GCCAGGTCAC TATGACAGTC 
CAGGTGTCAG GAAACCCACC TCCAGAAGTC ATATGGCTTC ACGACGGGAA CGAGATCCAG 
GAGTCAGAGG ACTTCCACTT CGAGCAGAAA GGTGGCTGGC ACAGCCTATG TATCCAGGAA 
GTGTTCCCGG AGGACACGGG CACATACACC TGTGAGGCCT GGAACAGCGC TGGGGAGGTC 
CGCACCCAGG CCATACTCAC CGTGCAAGAG CCCCATGATG GCACCCAGCC CTGGTTCATC 
AGCAAGCCTC GTTCGGTGAC AGCCTCCCTG GGCCAGAGTG TCCTCATCTC CTGCGCCATA 
GCTGGAGACC CCTTTCCCAC TGTGCACTGG CTCCGAGATG GCAAAGCCCT CTCCAGGGAC 
CATGGCCACT TTGAGCTGCT CCAGAATGAG GACGTGTTCA CCCTGGTTCT AAAGAACGTG 
CAGCCCTGGC ATGCCGGCCA GTATGAGATC CTGCTCAAGA ACCGGGTTGG CGAGTGCAGT 
TGCCAAGTGT CACTGATGCT GCACAAAGGT CCTTCCAGAG TCCCACCACC ACGGGGGCGG 
GAGCCTGCCA GCTGTGAGGG CCTCTGTGGT GGAGGAGTTG GTGCTCATGG TGATGCTGAC 
CGTGATGGGA CCCTGAGGCC CTGCTGGCCA GCAAGAGGGC AGGGTTGGCC TGAGGAGGAA 
GACGGAGAAG ACGTGAGGGG GCTGTTGAAG AGACGGGTGG AGACCAGGCT GCACACAGAA 
GAGGCCATCC GCCAGCAGGA GGTGGGGCAG CTGGATTTTC GAGACCTCCT GGGAAAGAAG 
GTGAGCACAA AGACCGTATC AGAAGACGAC CTGAAGGACA TCCCAGCCGA GCAGATGGAT 
TTCCGCGCCA ACCTTCAGCG GCAAGTGAAG CCAAAGACTG TGTCTGAGGA AGAGAGGAAG 
GTGCATAGCC CCCAGCAGGT TGACTTCCGG TCTGTCCTAG CCAAGAAGGG GACCCCTAAG 
ACCCCTGTCC CTGAGAAGGT GCCACCTCAA AAAGCTGCCA CCCCAGACTT TCGCTCAGTG 
CTAGGTAGCA AGAAGAAGTC GCCTGCGGAG AATGGGGGCA ACACTGCTGA GGTCTTGAAT 
GTCAAGGCTG GGGAAAGCCC CACGCCTGCA GGCAATGCAC AGCCAGTCGG GGCCCTGAAA 
CCCATAGGCA ACGCCAAGCC TGCTGAGACC CTGAAGTCCG TGGGCAATGC CAAACCAGCA 
GAGACCCTGA AGCCCGTGGG GAATGCCAAG CCAGCAGAGA CCCTGAAACC CGTAGGTAAT 
GTCAAGCCTG CCGAGACCCT GAAGCCCATA GCCAACGCAC AGCCTTCAGG GTCCCCAAAA 
CCCATAGCCA ACGCACAGCC TATTGAGACC CAGAAGTCTG TGGCCAATGC CAAGTCTGCC 
GAGACCCCGA AACTAGCGGG GAAAGAAGAA CTCAAGGAGG TTAGGAATGA TGTGAACTGT 
AAGAAAGCGC ATGTCGGAGC CACCGGCAAT GAAAAAAGAC CTGAGAGCCA AGGCTCGGCC 
CCAGTTTTCA AGGAAAAGCT ACAAGATGTC CATGTGGCTG AGGGCGAGAA GCTGCTACTC 
CAGTGCCAGG TGACCTCTGA TCCTCCGGCC ACTGTCACCT GGACACTGAA TGGAAAGACA 
CTCAAGACCA CAAAATTCAT CATCCTCGCC CAAGAAGGCT CACTCTTCTC CGTCTCCATC 
GAGAAGGCAC TGCCTGAGGA CAGGGGTCTA TACAAGTGTG TAGCCAAGAA CAGCGCTGGC 
CAGGCGGAAT GTTCCTGCCA AGTCACTGTG GACGATGCTG GGACCAGCGA GAACACCAAG 
GCCCCGGAGA TGAAATCCCG GAGACCCAAG AGCTCTCTTA CGCCTGTGCT AGGAACGGAA 
AGTGATGCTA CTGTGAAAAA GAAACCTGCC TCCAAGACAC CTACAAAAGC AGCTATGCCT 
CCTCAGATCA TCCAGTTCCC TGAGGACCAG AAGGTGCGTG CTGGAGAGCC AGTGGAGCTG 
TTTGGGAAAG TGACCGGTAC CCAGCCCATC ACCTGCACGT GGATGAAGTT CCGAAAGCAG 
ATCCAGGAGA GCGAGAACAT CAGGGTGGAG AACAGTGAGA GCGGCAGCAA GCTCACCATC 
CTGGCTGCAC GGCAGGAGCA CTGTGGCTGC TACACACTGG TGGTGGAGAA CAAGCTGAGC 
AGCAGACAGG CCCAAGTCAA CCTCACGGTT GTGGATAAGC CAGACCCCCC GGCTGGCACA 
CCTTGTGCTT CTGACATACG GAGTTCCTCG CTCACCCTGT CCTGGTATGG CTCCTCGTAT 
GATGGGGGCA GTGCCGTACA GTCCTACAAT GTTGAGATCT GGGACACGGA GGACAAGGTG 
TGGACAGAGC TAGCCACGTG CCGCAGCACC TCTTTCAATG TCCAAGACCT GCTGCCTGAC 
CGCGAGTATA AATTCCGAGT ACGTGCAGTC AACGTCTACG GAACCAGCGA GCCGAGCCAG 
GAGTCTGAGC TCACCGCAGT GGGAGAGAAG CCTGAAGAGC CAAAGGATGA AGTGGAAGTG 
TCCGATGATG ACGAAAAGGA GCCTGAGGTT GATTACCGGA CGGTGACGGT GAACACTGAA 
CAGAAAGTAT CTGATGTATA TGACATTGAA GAGCGACTAG GATCTGGGAA ATTTGGACAA 
GTCTTCCGAC TTGTTGAAAA GAGAACTGGA AAAATCTGGG CAGGGAAATT TTTCAAGGCC 
TATTCCGCCA AGGAGAAAGA AAACATCCGT CAGGAGATCA GCATCATGAA CTGTCTCCAC 
CACCCCAAGC TGGTCCAGTG CGTGGATGCC TTCGAAGAAA AGGCCAACAT CGTCATGGTT 
CTGGAGATTG TGTCAGGGGG TGAGCTATTT GAGCGCATCA TTGATGAGGA CTTCGAGCTA 
ACAGAGCGGG AGTGCATCAA GTACATGAGG CAGATTTCGG AAGGGGTGGA GTACATCCAC 
AAGCAGGGCA TCGTGCATCT GGACCTCAAG CCTGAGAACA TTATGTGTGT CAACAAGACA 
GGCACCAGGA TCAAGCTCAT TGACTTCGGC CTGGCACGAA GACTCGAGAA CGCTGGGTCT 
CTGAAGGTCC TCTTTGGGAC CCCAGAGTTT GTGGCTCCTG AGGTGATCAA CTATGAGCCC 
ATCGGCTACG CCACAGACAT GTGGAGCATC GGGGTCATCT GCTATATTCT GGTCAGCGGA 
CTTTCCCCCT TCATGGGTGA CAATGATAAC GAAACTTTAG CCAATGTCAC TTCAGCTACC 
TGGGACTTCG ACGACGAGGC GTTCGATGAG ATCTCGGAAG ATGCGAAGGA CTTCATCAGT 
AACTTGCTAA AGAAAGATAT GAAAAACCGT CTGGACTGCA CCCAGTGCCT TCAGCACCCA 
TGGCTGATGA AAGACACCAA GAACATGGAG GCCAAGAAGC TGTCCAAGGA CCGGATGAAG 
AAATACATGG CAAGAAGGAA GTGGCAGAAA ACAGGCAATG CTGTGAGAGC CATTGGGAGA 
CTGTCCTCTA TGGCAATGAT CTCAGGGCTC AGTGGCAGGA AATCTTCAAC AGGGTCACCA 
ACCAGCCCGA TCAATGCAGA GAAGCTAGAG TCTGAAGAAG ATGTCTCCCA GGCCTTCCTC 
GAGGCTGTTG CTGAGGAAAA GCCCCATGTA AAGCCCTACT TCTCTAAGAC CATTCGTGAC 
CTGGAGGTTG TGGAGGGAAG CGCTGCTAGA TTTGACTGCA AGATCGAAGG ATACCCAGAC 
CCTGAGGTTG TCTGGTTCAA GGATGACCAG TCAATCAGGG AGTCCCGCCA CTTCCAGATA 
GACTACGATG AAGACGGGAA CTGCTCGCTA ATTATCAGTG ATGTCTGTGG GGATGATGAC 
GCCAAGTACA CCTGCAAGGC TGTCAACAGC CTCGGAGAAG CCACCTGCAC CGCGGAGCTC 
ATCGTGGAGA CCATGGAGGA AGGGGAAGGG GAGGAGGGGG GGGAGGAGGA GGAGGAGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_017597921.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453410.1 Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X3, mRNA.

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CloneID	ORa34329
Clone ID Related Accession (Same CDS sequence)	XM_017597921.1 , XM_006248434.3 , XM_006248433.3
Accession Version	XM_017597921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA 
GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG 
CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC 
AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG 
GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC 
CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG 
GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC 
AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG 
GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA 
GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG 
AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA 
GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT 
CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC 
ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC 
GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT 
CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT 
GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC 
CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA 
CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC 
ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC 
CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC 
TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG 
CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA 
GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA 
GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG 
AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC 
ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA 
GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC 
CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC 
TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT 
GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG 
ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA 
GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT 
GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG 
CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG 
AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG 
CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC 
GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG 
AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC 
CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT 
CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG 
GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT 
GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG 
ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC 
AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC 
ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG 
ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA 
GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC 
AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT 
GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG 
GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC 
GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT 
CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT 
GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC 
AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT 
GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC 
CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC 
ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG 
GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC 
TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG 
GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC 
TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC 
AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC 
ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA 
GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG 
AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG 
GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT 
GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT 
GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC 
CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT 
GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA 
TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG 
AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC 
AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC 
GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG 
TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC 
ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT 
AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT 
GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC 
CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG 
GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG 
AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG 
CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA 
GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT 
GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT 
AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC 
CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG 
CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC 
AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA 
GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453410.1
CDS	5..5863
Translation	MGDVKLFASSHMSKTSHSVDPSRVSSLPLTEAPAFILPPRNLCVKEGATAKFEGRVRGHPEPQVTWHRNGQTITNGGRFLLDGGVRGTFSLVIRGVREEDKGKYTCEASNGSGARQVTVELTVEGDLMKKHGQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRLSRGTKDPSPDIRKEVTNGISKDPETVAESKNCSSPQKSNFLAQATNSHLKSLQQPKLKPCEDSPRKVLQSSVLQKTSSTIVLQASKVQPEVRAAGIGASGPGEERKSLTAPRQATPPTRQCGLGGSVGNKFITGNIPIENQRESRFPKFESQPQSQEVTEGQTVKFTCEVSGIPKPEVGWFLEGIPVRRQEGIVEIYEDGTSHYLCLPRAGTRDSGRYSCTASNTLGQVSCSWTLRVDRPSLTQTAPSFSSVLKDSVVIEGQDFVLRCSVRGTPAPQVTWLLNGQPIQFARSICEAGEAELHIQDALPEDRGTYTCLAENALGQVSCSATVTVQEKKGEGERAHRLPPAPSKLFAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKALSRDHGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHKGPSRVPPPRGREPASCEGLCGGGVGAHGDADRDGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKVPPQKAATPDFRSVLGSKKKSPAENGGNTAEVLNVKAGESPTPAGNAQPVGALKPIGNAKPAETLKSVGNAKPAETLKPVGNAKPAETLKPVGNVKPAETLKPIANAQPSGSPKPIANAQPIETQKSVANAKSAETPKLAGKEELKEVRNDVNCKKAHVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILAQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAGTSENTKAPEMKSRRPKSSLTPVLGTESDATVKKKPASKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESENIRVENSESGSKLTILAARQEHCGCYTLVVENKLSSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWTELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKRTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISEDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEE

Target ORF information:

RefSeq Version	XM_017597921.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597921.1

ORF Insert Sequence:

ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA 
GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG 
CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC 
AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG 
GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC 
CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG 
GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC 
AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG 
GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA 
GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG 
AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA 
GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT 
CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC 
ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC 
GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT 
CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT 
GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC 
CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA 
CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC 
ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC 
CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC 
TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG 
CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA 
GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA 
GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG 
AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC 
ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA 
GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC 
CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC 
TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT 
GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG 
ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA 
GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT 
GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG 
CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG 
AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG 
CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC 
GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG 
AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC 
CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT 
CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG 
GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT 
GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG 
ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC 
AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC 
ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG 
ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA 
GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC 
AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT 
GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG 
GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC 
GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT 
CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT 
GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC 
AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT 
GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC 
CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC 
ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG 
GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC 
TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG 
GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC 
TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC 
AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC 
ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA 
GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG 
AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG 
GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT 
GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT 
GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC 
CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT 
GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA 
TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG 
AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC 
AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC 
GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG 
TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC 
ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT 
AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT 
GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC 
CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG 
GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG 
AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG 
CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA 
GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT 
GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT 
AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC 
CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG 
CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC 
AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA 
GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_006248434.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248496.1 Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X2, mRNA.

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CloneID	ORa34329
Clone ID Related Accession (Same CDS sequence)	XM_017597921.1 , XM_006248434.3 , XM_006248433.3
Accession Version	XM_006248434.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248434.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA 
GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG 
CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC 
AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG 
GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC 
CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG 
GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC 
AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG 
GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA 
GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG 
AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA 
GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT 
CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC 
ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC 
GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT 
CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT 
GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC 
CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA 
CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC 
ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC 
CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC 
TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG 
CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA 
GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA 
GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG 
AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC 
ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA 
GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC 
CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC 
TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT 
GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG 
ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA 
GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT 
GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG 
CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG 
AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG 
CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC 
GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG 
AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC 
CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT 
CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG 
GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT 
GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG 
ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC 
AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC 
ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG 
ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA 
GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC 
AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT 
GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG 
GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC 
GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT 
CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT 
GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC 
AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT 
GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC 
CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC 
ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG 
GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC 
TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG 
GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC 
TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC 
AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC 
ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA 
GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG 
AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG 
GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT 
GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT 
GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC 
CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT 
GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA 
TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG 
AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC 
AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC 
GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG 
TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC 
ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT 
AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT 
GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC 
CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG 
GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG 
AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG 
CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA 
GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT 
GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT 
AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC 
CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG 
CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC 
AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA 
GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248496.1
CDS	160..6018
Translation	MGDVKLFASSHMSKTSHSVDPSRVSSLPLTEAPAFILPPRNLCVKEGATAKFEGRVRGHPEPQVTWHRNGQTITNGGRFLLDGGVRGTFSLVIRGVREEDKGKYTCEASNGSGARQVTVELTVEGDLMKKHGQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRLSRGTKDPSPDIRKEVTNGISKDPETVAESKNCSSPQKSNFLAQATNSHLKSLQQPKLKPCEDSPRKVLQSSVLQKTSSTIVLQASKVQPEVRAAGIGASGPGEERKSLTAPRQATPPTRQCGLGGSVGNKFITGNIPIENQRESRFPKFESQPQSQEVTEGQTVKFTCEVSGIPKPEVGWFLEGIPVRRQEGIVEIYEDGTSHYLCLPRAGTRDSGRYSCTASNTLGQVSCSWTLRVDRPSLTQTAPSFSSVLKDSVVIEGQDFVLRCSVRGTPAPQVTWLLNGQPIQFARSICEAGEAELHIQDALPEDRGTYTCLAENALGQVSCSATVTVQEKKGEGERAHRLPPAPSKLFAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKALSRDHGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHKGPSRVPPPRGREPASCEGLCGGGVGAHGDADRDGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKVPPQKAATPDFRSVLGSKKKSPAENGGNTAEVLNVKAGESPTPAGNAQPVGALKPIGNAKPAETLKSVGNAKPAETLKPVGNAKPAETLKPVGNVKPAETLKPIANAQPSGSPKPIANAQPIETQKSVANAKSAETPKLAGKEELKEVRNDVNCKKAHVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILAQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAGTSENTKAPEMKSRRPKSSLTPVLGTESDATVKKKPASKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESENIRVENSESGSKLTILAARQEHCGCYTLVVENKLSSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWTELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKRTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISEDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEE

Target ORF information:

RefSeq Version	XM_006248434.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248434.3

ORF Insert Sequence:

ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA 
GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG 
CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC 
AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG 
GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC 
CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG 
GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC 
AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG 
GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA 
GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG 
AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA 
GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT 
CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC 
ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC 
GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT 
CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT 
GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC 
CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA 
CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC 
ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC 
CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC 
TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG 
CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA 
GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA 
GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG 
AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC 
ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA 
GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC 
CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC 
TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT 
GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG 
ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA 
GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT 
GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG 
CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG 
AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG 
CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC 
GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG 
AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC 
CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT 
CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG 
GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT 
GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG 
ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC 
AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC 
ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG 
ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA 
GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC 
AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT 
GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG 
GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC 
GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT 
CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT 
GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC 
AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT 
GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC 
CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC 
ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG 
GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC 
TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG 
GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC 
TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC 
AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC 
ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA 
GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG 
AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG 
GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT 
GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT 
GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC 
CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT 
GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA 
TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG 
AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC 
AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC 
GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG 
TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC 
ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT 
AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT 
GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC 
CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG 
GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG 
AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG 
CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA 
GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT 
GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT 
AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC 
CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG 
CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC 
AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA 
GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_006248433.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248495.1 Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X1, mRNA.

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CloneID	ORa34329
Clone ID Related Accession (Same CDS sequence)	XM_017597921.1 , XM_006248434.3 , XM_006248433.3
Accession Version	XM_006248433.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248433.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA 
GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG 
CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC 
AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG 
GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC 
CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG 
GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC 
AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG 
GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA 
GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG 
AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA 
GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT 
CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC 
ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC 
GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT 
CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT 
GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC 
CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA 
CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC 
ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC 
CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC 
TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG 
CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA 
GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA 
GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG 
AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC 
ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA 
GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC 
CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC 
TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT 
GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG 
ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA 
GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT 
GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG 
CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG 
AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG 
CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC 
GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG 
AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC 
CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT 
CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG 
GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT 
GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG 
ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC 
AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC 
ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG 
ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA 
GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC 
AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT 
GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG 
GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC 
GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT 
CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT 
GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC 
AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT 
GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC 
CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC 
ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG 
GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC 
TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG 
GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC 
TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC 
AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC 
ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA 
GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG 
AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG 
GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT 
GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT 
GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC 
CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT 
GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA 
TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG 
AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC 
AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC 
GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG 
TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC 
ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT 
AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT 
GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC 
CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG 
GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG 
AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG 
CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA 
GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT 
GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT 
AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC 
CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG 
CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC 
AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA 
GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248495.1
CDS	369..6227
Translation	MGDVKLFASSHMSKTSHSVDPSRVSSLPLTEAPAFILPPRNLCVKEGATAKFEGRVRGHPEPQVTWHRNGQTITNGGRFLLDGGVRGTFSLVIRGVREEDKGKYTCEASNGSGARQVTVELTVEGDLMKKHGQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRLSRGTKDPSPDIRKEVTNGISKDPETVAESKNCSSPQKSNFLAQATNSHLKSLQQPKLKPCEDSPRKVLQSSVLQKTSSTIVLQASKVQPEVRAAGIGASGPGEERKSLTAPRQATPPTRQCGLGGSVGNKFITGNIPIENQRESRFPKFESQPQSQEVTEGQTVKFTCEVSGIPKPEVGWFLEGIPVRRQEGIVEIYEDGTSHYLCLPRAGTRDSGRYSCTASNTLGQVSCSWTLRVDRPSLTQTAPSFSSVLKDSVVIEGQDFVLRCSVRGTPAPQVTWLLNGQPIQFARSICEAGEAELHIQDALPEDRGTYTCLAENALGQVSCSATVTVQEKKGEGERAHRLPPAPSKLFAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKALSRDHGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHKGPSRVPPPRGREPASCEGLCGGGVGAHGDADRDGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKVPPQKAATPDFRSVLGSKKKSPAENGGNTAEVLNVKAGESPTPAGNAQPVGALKPIGNAKPAETLKSVGNAKPAETLKPVGNAKPAETLKPVGNVKPAETLKPIANAQPSGSPKPIANAQPIETQKSVANAKSAETPKLAGKEELKEVRNDVNCKKAHVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILAQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAGTSENTKAPEMKSRRPKSSLTPVLGTESDATVKKKPASKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESENIRVENSESGSKLTILAARQEHCGCYTLVVENKLSSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWTELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKRTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISEDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEE

Target ORF information:

RefSeq Version	XM_006248433.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248433.3

ORF Insert Sequence:

ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA 
GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG 
CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC 
AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG 
GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC 
CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG 
GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC 
AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG 
GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA 
GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG 
AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA 
GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT 
CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC 
ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC 
GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT 
CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT 
GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC 
CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA 
CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC 
ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC 
CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC 
TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG 
CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA 
GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA 
GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG 
AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC 
ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA 
GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC 
CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC 
TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT 
GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG 
ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA 
GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT 
GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG 
CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG 
AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG 
CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC 
GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG 
AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC 
CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT 
CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG 
GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT 
GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG 
ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC 
AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC 
ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG 
ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA 
GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC 
AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT 
GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG 
GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC 
GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT 
CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT 
GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC 
AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT 
GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC 
CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC 
ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG 
GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC 
TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG 
GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC 
TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC 
AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC 
ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA 
GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG 
AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG 
GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT 
GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT 
GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC 
CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT 
GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA 
TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG 
AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC 
AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC 
GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG 
TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC 
ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT 
AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT 
GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC 
CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG 
GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG 
AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG 
CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA 
GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT 
GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT 
AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC 
CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG 
CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC 
AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA 
GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_008768711.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766933.1 Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X4, mRNA.

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CloneID	ORa44660
Clone ID Related Accession (Same CDS sequence)	XM_008768711.2
Accession Version	XM_008768711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin light chain kinase, smooth muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA 
GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG 
CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC 
AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG 
GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC 
CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG 
GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC 
AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG 
GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA 
GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG 
AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA 
GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT 
CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC 
ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC 
GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT 
CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT 
GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC 
CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA 
CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC 
ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC 
CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC 
TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG 
CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA 
GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA 
GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG 
AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC 
ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA 
GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC 
CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC 
TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT 
GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG 
ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA 
GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT 
GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG 
CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG 
AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG 
CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC 
GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG 
AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC 
CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT 
CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG 
GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT 
GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG 
ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC 
AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC 
ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG 
ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA 
GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC 
AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT 
GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG 
GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC 
GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT 
CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT 
GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC 
AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT 
GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC 
CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC 
ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG 
GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC 
TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG 
GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC 
TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC 
AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC 
ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA 
GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG 
AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG 
GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT 
GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT 
GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC 
CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT 
GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA 
TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG 
AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC 
AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC 
GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG 
TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC 
ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT 
AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT 
GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC 
CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG 
GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG 
AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG 
CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA 
GAGTCTGAAG ATGTCTCCCA GGCCTTCCTC GAGGCTGTTG CTGAGGAAAA GCCCCATGTA 
AAGCCCTACT TCTCTAAGAC CATTCGTGAC CTGGAGGTTG TGGAGGGAAG CGCTGCTAGA 
TTTGACTGCA AGATCGAAGG ATACCCAGAC CCTGAGGTTG TCTGGTTCAA GGATGACCAG 
TCAATCAGGG AGTCCCGCCA CTTCCAGATA GACTACGATG AAGACGGGAA CTGCTCGCTA 
ATTATCAGTG ATGTCTGTGG GGATGATGAC GCCAAGTACA CCTGCAAGGC TGTCAACAGC 
CTCGGAGAAG CCACCTGCAC CGCGGAGCTC ATCGTGGAGA CCATGGAGGA AGGGGAAGGG 
GAGGAGGGGG GGGAGGAGGA GGAGGAGGAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766933.1
CDS	370..6225
Translation	MGDVKLFASSHMSKTSHSVDPSRVSSLPLTEAPAFILPPRNLCVKEGATAKFEGRVRGHPEPQVTWHRNGQTITNGGRFLLDGGVRGTFSLVIRGVREEDKGKYTCEASNGSGARQVTVELTVEGDLMKKHGQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRLSRGTKDPSPDIRKEVTNGISKDPETVAESKNCSSPQKSNFLAQATNSHLKSLQQPKLKPCEDSPRKVLQSSVLQKTSSTIVLQASKVQPEVRAAGIGASGPGEERKSLTAPRQATPPTRQCGLGGSVGNKFITGNIPIENQRESRFPKFESQPQSQEVTEGQTVKFTCEVSGIPKPEVGWFLEGIPVRRQEGIVEIYEDGTSHYLCLPRAGTRDSGRYSCTASNTLGQVSCSWTLRVDRPSLTQTAPSFSSVLKDSVVIEGQDFVLRCSVRGTPAPQVTWLLNGQPIQFARSICEAGEAELHIQDALPEDRGTYTCLAENALGQVSCSATVTVQEKKGEGERAHRLPPAPSKLFAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKALSRDHGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHKGPSRVPPPRGREPASCEGLCGGGVGAHGDADRDGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKVPPQKAATPDFRSVLGSKKKSPAENGGNTAEVLNVKAGESPTPAGNAQPVGALKPIGNAKPAETLKSVGNAKPAETLKPVGNAKPAETLKPVGNVKPAETLKPIANAQPSGSPKPIANAQPIETQKSVANAKSAETPKLAGKEELKEVRNDVNCKKAHVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILAQEGSLFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAGTSENTKAPEMKSRRPKSSLTPVLGTESDATVKKKPASKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESENIRVENSESGSKLTILAARQEHCGCYTLVVENKLSSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWTELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKRTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISEDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEE

Target ORF information:

RefSeq Version	XM_008768711.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768711.2

ORF Insert Sequence:

ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA 
GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG 
CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC 
AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG 
GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC 
CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG 
GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC 
AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG 
GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA 
GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG 
AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA 
GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT 
CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC 
ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC 
GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT 
CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT 
GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC 
CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA 
CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC 
ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC 
CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC 
TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG 
CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA 
GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA 
GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG 
AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC 
ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA 
GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC 
CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC 
TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT 
GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG 
ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA 
GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT 
GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG 
CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG 
AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG 
CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC 
GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG 
AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC 
CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT 
CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG 
GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT 
GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG 
ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC 
AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC 
ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG 
ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA 
GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC 
AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT 
GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG 
GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC 
GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT 
CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT 
GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC 
AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT 
GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC 
CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC 
ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG 
GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC 
TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG 
GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC 
TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC 
AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC 
ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA 
GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG 
AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG 
GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT 
GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT 
GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC 
CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT 
GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA 
TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG 
AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC 
AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC 
GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG 
TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC 
ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT 
AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT 
GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC 
CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG 
GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG 
AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG 
CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA 
GAGTCTGAAG ATGTCTCCCA GGCCTTCCTC GAGGCTGTTG CTGAGGAAAA GCCCCATGTA 
AAGCCCTACT TCTCTAAGAC CATTCGTGAC CTGGAGGTTG TGGAGGGAAG CGCTGCTAGA 
TTTGACTGCA AGATCGAAGG ATACCCAGAC CCTGAGGTTG TCTGGTTCAA GGATGACCAG 
TCAATCAGGG AGTCCCGCCA CTTCCAGATA GACTACGATG AAGACGGGAA CTGCTCGCTA 
ATTATCAGTG ATGTCTGTGG GGATGATGAC GCCAAGTACA CCTGCAAGGC TGTCAACAGC 
CTCGGAGAAG CCACCTGCAC CGCGGAGCTC ATCGTGGAGA CCATGGAGGA AGGGGAAGGG 
GAGGAGGGGG GGGAGGAGGA GGAGGAGGAG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Angiotensin II Facilitates Matrix Metalloproteinase-9-Mediated Myosin Light Chain Kinase Degradation in Pressure Overload-Induced Cardiac Hypertrophy.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology44(6)2281-2295(2017)
Wang S,Cheng M,Hu Z,Hu S,Zou Q,Lai X,Liu B,Jiang H,Huang C,Wu G

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Nature immunology14(8)858-66(2013 Aug)
Liang Y,Cucchetti M,Roncagalli R,Yokosuka T,Malzac A,Bertosio E,Imbert J,Nijman IJ,Suchanek M,Saito T,W?lfing C,Malissen B,Malissen M

Myosin light chain kinase is involved in the mechanism of gastrointestinal dysfunction in diabetic rats.
Digestive diseases and sciences57(5)1197-202(2012 May)
Hu W,Feng P

Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells.
Journal of pharmacological sciences116(1)116-27(2011)
Thatcher SE,Fultz ME,Tanaka H,Hagiwara H,Zhang HL,Zhang Y,Hayakawa K,Yoshiyama S,Nakamura A,Wang HH,Katayama T,Watanabe M,Lin Y,Wright GL,Kohama K

A family of protein-deglutamylating enzymes associated with neurodegeneration.
Cell143(4)564-78(2010 Nov)
Rogowski K,van Dijk J,Magiera MM,Bosc C,Deloulme JC,Bosson A,Peris L,Gold ND,Lacroix B,Bosch Grau M,Bec N,Larroque C,Desagher S,Holzer M,Andrieux A,Moutin MJ,Janke C

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Mylk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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