Mylk cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Mylk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mylk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa44661 XM_008768712.2
Latest version!
Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
ORa04367 NM_001105874.2
Latest version!
Rattus norvegicus myosin light chain kinase (Mylk), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa34329 XM_017597921.1
Latest version!
Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa34329 XM_006248434.3
Latest version!
Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa34329 XM_006248433.3
Latest version!
Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa44660 XM_008768711.2
Latest version!
Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa44661
    Clone ID Related Accession (Same CDS sequence) XM_008768712.2
    Accession Version XM_008768712.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGACCCAA GAGCTCTCTT ACGCCTGTGC TAGGAACGGA AAGTGATGCT 
    ACTGTGAAAA AGAAACCTGC CTCCAAGACA CCTACAAAAG CAGCTATGCC TCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTGCGT GCTGGAGAGC CAGTGGAGCT GTTTGGGAAA
    GTGACCGGTA CCCAGCCCAT CACCTGCACG TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGCGAGAACA TCAGGGTGGA GAACAGTGAG AGCGGCAGCA AGCTCACCAT CCTGGCTGCA
    CGGCAGGAGC ACTGTGGCTG CTACACACTG GTGGTGGAGA ACAAGCTGAG CAGCAGACAG
    GCCCAAGTCA ACCTCACGGT TGTGGATAAG CCAGACCCCC CGGCTGGCAC ACCTTGTGCT
    TCTGACATAC GGAGTTCCTC GCTCACCCTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGTGCCGTAC AGTCCTACAA TGTTGAGATC TGGGACACGG AGGACAAGGT GTGGACAGAG
    CTAGCCACGT GCCGCAGCAC CTCTTTCAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAT
    AAATTCCGAG TACGTGCAGT CAACGTCTAC GGAACCAGCG AGCCGAGCCA GGAGTCTGAG
    CTCACCGCAG TGGGAGAGAA GCCTGAAGAG CCAAAGGATG AAGTGGAAGT GTCCGATGAT
    GACGAAAAGG AGCCTGAGGT TGATTACCGG ACGGTGACGG TGAACACTGA ACAGAAAGTA
    TCTGATGTAT ATGACATTGA AGAGCGACTA GGATCTGGGA AATTTGGACA AGTCTTCCGA
    CTTGTTGAAA AGAGAACTGG AAAAATCTGG GCAGGGAAAT TTTTCAAGGC CTATTCCGCC
    AAGGAGAAAG AAAACATCCG TCAGGAGATC AGCATCATGA ACTGTCTCCA CCACCCCAAG
    CTGGTCCAGT GCGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT TCTGGAGATT
    GTGTCAGGGG GTGAGCTATT TGAGCGCATC ATTGATGAGG ACTTCGAGCT AACAGAGCGG
    GAGTGCATCA AGTACATGAG GCAGATTTCG GAAGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCATC TGGACCTCAA GCCTGAGAAC ATTATGTGTG TCAACAAGAC AGGCACCAGG
    ATCAAGCTCA TTGACTTCGG CCTGGCACGA AGACTCGAGA ACGCTGGGTC TCTGAAGGTC
    CTCTTTGGGA CCCCAGAGTT TGTGGCTCCT GAGGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTATATTC TGGTCAGCGG ACTTTCCCCC
    TTCATGGGTG ACAATGATAA CGAAACTTTA GCCAATGTCA CTTCAGCTAC CTGGGACTTC
    GACGACGAGG CGTTCGATGA GATCTCGGAA GATGCGAAGG ACTTCATCAG TAACTTGCTA
    AAGAAAGATA TGAAAAACCG TCTGGACTGC ACCCAGTGCC TTCAGCACCC ATGGCTGATG
    AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGGATGAA GAAATACATG
    GCAAGAAGGA AGTGGCAGAA AACAGGCAAT GCTGTGAGAG CCATTGGGAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCTTCAA CAGGGTCACC AACCAGCCCG
    ATCAATGCAG AGAAGCTAGA GTCTGAAGAA GATGTCTCCC AGGCCTTCCT CGAGGCTGTT
    GCTGAGGAAA AGCCCCATGT AAAGCCCTAC TTCTCTAAGA CCATTCGTGA CCTGGAGGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATCGAAG GATACCCAGA CCCTGAGGTT
    GTCTGGTTCA AGGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAAGACGGGA ACTGCTCGCT AATTATCAGT GATGTCTGTG GGGATGATGA CGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CCGCGGAGCT CATCGTGGAG
    ACCATGGAGG AAGGGGAAGG GGAGGAGGGG GGGGAGGAGG AGGAGGAGGA GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008766934.1
    CDS489..2645
    Translation

    Target ORF information:

    RefSeq Version XM_008768712.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008768712.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGACCCAA GAGCTCTCTT ACGCCTGTGC TAGGAACGGA AAGTGATGCT 
    ACTGTGAAAA AGAAACCTGC CTCCAAGACA CCTACAAAAG CAGCTATGCC TCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTGCGT GCTGGAGAGC CAGTGGAGCT GTTTGGGAAA
    GTGACCGGTA CCCAGCCCAT CACCTGCACG TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGCGAGAACA TCAGGGTGGA GAACAGTGAG AGCGGCAGCA AGCTCACCAT CCTGGCTGCA
    CGGCAGGAGC ACTGTGGCTG CTACACACTG GTGGTGGAGA ACAAGCTGAG CAGCAGACAG
    GCCCAAGTCA ACCTCACGGT TGTGGATAAG CCAGACCCCC CGGCTGGCAC ACCTTGTGCT
    TCTGACATAC GGAGTTCCTC GCTCACCCTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGTGCCGTAC AGTCCTACAA TGTTGAGATC TGGGACACGG AGGACAAGGT GTGGACAGAG
    CTAGCCACGT GCCGCAGCAC CTCTTTCAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAT
    AAATTCCGAG TACGTGCAGT CAACGTCTAC GGAACCAGCG AGCCGAGCCA GGAGTCTGAG
    CTCACCGCAG TGGGAGAGAA GCCTGAAGAG CCAAAGGATG AAGTGGAAGT GTCCGATGAT
    GACGAAAAGG AGCCTGAGGT TGATTACCGG ACGGTGACGG TGAACACTGA ACAGAAAGTA
    TCTGATGTAT ATGACATTGA AGAGCGACTA GGATCTGGGA AATTTGGACA AGTCTTCCGA
    CTTGTTGAAA AGAGAACTGG AAAAATCTGG GCAGGGAAAT TTTTCAAGGC CTATTCCGCC
    AAGGAGAAAG AAAACATCCG TCAGGAGATC AGCATCATGA ACTGTCTCCA CCACCCCAAG
    CTGGTCCAGT GCGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT TCTGGAGATT
    GTGTCAGGGG GTGAGCTATT TGAGCGCATC ATTGATGAGG ACTTCGAGCT AACAGAGCGG
    GAGTGCATCA AGTACATGAG GCAGATTTCG GAAGGGGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCATC TGGACCTCAA GCCTGAGAAC ATTATGTGTG TCAACAAGAC AGGCACCAGG
    ATCAAGCTCA TTGACTTCGG CCTGGCACGA AGACTCGAGA ACGCTGGGTC TCTGAAGGTC
    CTCTTTGGGA CCCCAGAGTT TGTGGCTCCT GAGGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTATATTC TGGTCAGCGG ACTTTCCCCC
    TTCATGGGTG ACAATGATAA CGAAACTTTA GCCAATGTCA CTTCAGCTAC CTGGGACTTC
    GACGACGAGG CGTTCGATGA GATCTCGGAA GATGCGAAGG ACTTCATCAG TAACTTGCTA
    AAGAAAGATA TGAAAAACCG TCTGGACTGC ACCCAGTGCC TTCAGCACCC ATGGCTGATG
    AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGGATGAA GAAATACATG
    GCAAGAAGGA AGTGGCAGAA AACAGGCAAT GCTGTGAGAG CCATTGGGAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCTTCAA CAGGGTCACC AACCAGCCCG
    ATCAATGCAG AGAAGCTAGA GTCTGAAGAA GATGTCTCCC AGGCCTTCCT CGAGGCTGTT
    GCTGAGGAAA AGCCCCATGT AAAGCCCTAC TTCTCTAAGA CCATTCGTGA CCTGGAGGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATCGAAG GATACCCAGA CCCTGAGGTT
    GTCTGGTTCA AGGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAAGACGGGA ACTGCTCGCT AATTATCAGT GATGTCTGTG GGGATGATGA CGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CCGCGGAGCT CATCGTGGAG
    ACCATGGAGG AAGGGGAAGG GGAGGAGGGG GGGGAGGAGG AGGAGGAGGA GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa04367
    Clone ID Related Accession (Same CDS sequence) NM_001105874.2
    Accession Version NM_001105874.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5886bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-27
    Organism Rattus norvegicus(Norway rat)
    Product myosin light chain kinase, smooth muscle
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07034409.1 and AABR07034408.1. On Jul 9, 2010 this sequence version replaced NM_001105874.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because insufficient rat transcript evidence was available to represent the gene. The genomic coordinates used for the transcript record were based on alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTGGCC AGTTACCTGG GAAGACCATG GGGGACGTGA AACTGTTTGC CTCGTCGCAC 
    ATGTCCAAAA CCTCCCACAG TGTGGATCCC TCGAGGGTCA GCTCTCTGCC TCTCACCGAG
    GCCCCTGCTT TCATTCTGCC TCCTCGGAAC CTCTGCGTCA AAGAAGGAGC CACCGCCAAG
    TTTGAAGGGA GGGTCCGGGG CCACCCAGAG CCCCAGGTGA CATGGCACAG AAATGGGCAA
    ACCATCACCA ATGGCGGCCG CTTCCTGCTG GACGGTGGGG TGCGGGGGAC TTTCAGCCTC
    GTCATCCGTG GTGTCCGTGA GGAGGACAAG GGCAAGTACA CCTGTGAAGC TAGCAACGGC
    AGTGGCGCCC GCCAGGTGAC CGTGGAGCTG ACTGTGGAAG GGGATCTTAT GAAGAAGCAT
    GGTCAGCCCG TTCTTTCTAA AGCCTCAGGA TTTCCAGGGG AGACTCGTCC TAGCATCTGG
    GGTGAGTGCC CGCCAAAGTT TGCCACCAAG CTGGGCAGAG CTGTGGTCAA AGAAGGGCAG
    ATGGGGCGAT TTTCCTGCAA GATCACTGGC CGGCCTCCAC CACAGGTCAC CTGGCTCAAG
    GGAAATGTCC CGTTGCAGCC AAGTGCCCGA GTGTCTATGT CAGAGAAGAA TGGCATGCAG
    ATCCTGGAAA TCCACGAGGT CACTCAGGAC GACATGGGCA CGTACACATG CATGGTGGTG
    AATGGGTCAG GGAAAGCCTC CATGTCAGCG GAGCTCTCCA TCCCAGGTTT GGACAATGCC
    ACCAGGTTGT CAAGAGGAAC CAAGGACCCC AGTCCAGACA TCAGGAAGGA GGTGACCAAT
    GGAATCTCCA AGGACCCAGA GACTGTGGCT GAAAGTAAGA ACTGTTCAAG TCCCCAGAAG
    AGCAACTTCT TAGCCCAGGC CACCAACAGT CACCTCAAGT CCCTGCAGCA GCCAAAGCTG
    AAGCCGTGTG AAGATTCCCC CAGAAAGGTC CTGCAGTCCT CCGTCCTGCA GAAGACCTCC
    AGCACCATTG TTCTGCAGGC CTCAAAAGTC CAGCCAGAAG TGAGAGCAGC GGGCATAGGG
    GCCTCCGGTC CTGGGGAAGA GAGGAAGAGT CTGACTGCCC CTCGGCAAGC CACACCCCCT
    ACCAGACAGT GTGGCCTGGG AGGCTCAGTT GGGAACAAAT TTATCACGGG GAACATCCCC
    ATAGAGAACC AAAGGGAGTC AAGATTTCCC AAGTTTGAGA GTCAGCCCCA AAGCCAAGAA
    GTCACTGAAG GTCAAACAGT CAAGTTCACC TGTGAGGTTT CAGGGATACC AAAGCCTGAA
    GTGGGTTGGT TCCTGGAAGG CATCCCCGTG AGGAGACAAG AAGGCATCGT TGAGATTTAT
    GAAGACGGTA CATCCCATTA CCTGTGTCTG CCGAGAGCCG GGACGAGAGA CAGCGGGAGA
    TACAGCTGCA CAGCATCCAA TACCCTTGGC CAAGTGTCCT GCAGTTGGAC GCTCCGGGTG
    GACCGGCCAA GCTTGACGCA GACAGCCCCT TCCTTCTCTA GTGTTCTGAA GGACAGTGTT
    GTCATTGAAG GCCAGGATTT TGTGCTACGG TGCTCTGTAC GGGGGACCCC AGCACCCCAG
    GTCACTTGGC TGCTCAATGG GCAGCCCATC CAGTTTGCTC GATCCATCTG TGAGGCTGGC
    GAGGCCGAGC TCCACATCCA GGATGCCCTG CCCGAGGACC GTGGCACCTA CACCTGCCTG
    GCTGAGAATG CCTTGGGGCA GGTGTCCTGC AGTGCCACGG TCACGGTCCA AGAAAAGAAA
    GGCGAAGGAG AGAGGGCGCA CCGTCTGCCC CCAGCTCCCA GCAAGCTCTT CGCTCCCATC
    TTCCTGCAGG GTCTCTCTGA CCTCAAAGTC ATGGATGGAA GCCAGGTCAC TATGACAGTC
    CAGGTGTCAG GAAACCCACC TCCAGAAGTC ATATGGCTTC ACGACGGGAA CGAGATCCAG
    GAGTCAGAGG ACTTCCACTT CGAGCAGAAA GGTGGCTGGC ACAGCCTATG TATCCAGGAA
    GTGTTCCCGG AGGACACGGG CACATACACC TGTGAGGCCT GGAACAGCGC TGGGGAGGTC
    CGCACCCAGG CCATACTCAC CGTGCAAGAG CCCCATGATG GCACCCAGCC CTGGTTCATC
    AGCAAGCCTC GTTCGGTGAC AGCCTCCCTG GGCCAGAGTG TCCTCATCTC CTGCGCCATA
    GCTGGAGACC CCTTTCCCAC TGTGCACTGG CTCCGAGATG GCAAAGCCCT CTCCAGGGAC
    CATGGCCACT TTGAGCTGCT CCAGAATGAG GACGTGTTCA CCCTGGTTCT AAAGAACGTG
    CAGCCCTGGC ATGCCGGCCA GTATGAGATC CTGCTCAAGA ACCGGGTTGG CGAGTGCAGT
    TGCCAAGTGT CACTGATGCT GCACAAAGGT CCTTCCAGAG TCCCACCACC ACGGGGGCGG
    GAGCCTGCCA GCTGTGAGGG CCTCTGTGGT GGAGGAGTTG GTGCTCATGG TGATGCTGAC
    CGTGATGGGA CCCTGAGGCC CTGCTGGCCA GCAAGAGGGC AGGGTTGGCC TGAGGAGGAA
    GACGGAGAAG ACGTGAGGGG GCTGTTGAAG AGACGGGTGG AGACCAGGCT GCACACAGAA
    GAGGCCATCC GCCAGCAGGA GGTGGGGCAG CTGGATTTTC GAGACCTCCT GGGAAAGAAG
    GTGAGCACAA AGACCGTATC AGAAGACGAC CTGAAGGACA TCCCAGCCGA GCAGATGGAT
    TTCCGCGCCA ACCTTCAGCG GCAAGTGAAG CCAAAGACTG TGTCTGAGGA AGAGAGGAAG
    GTGCATAGCC CCCAGCAGGT TGACTTCCGG TCTGTCCTAG CCAAGAAGGG GACCCCTAAG
    ACCCCTGTCC CTGAGAAGGT GCCACCTCAA AAAGCTGCCA CCCCAGACTT TCGCTCAGTG
    CTAGGTAGCA AGAAGAAGTC GCCTGCGGAG AATGGGGGCA ACACTGCTGA GGTCTTGAAT
    GTCAAGGCTG GGGAAAGCCC CACGCCTGCA GGCAATGCAC AGCCAGTCGG GGCCCTGAAA
    CCCATAGGCA ACGCCAAGCC TGCTGAGACC CTGAAGTCCG TGGGCAATGC CAAACCAGCA
    GAGACCCTGA AGCCCGTGGG GAATGCCAAG CCAGCAGAGA CCCTGAAACC CGTAGGTAAT
    GTCAAGCCTG CCGAGACCCT GAAGCCCATA GCCAACGCAC AGCCTTCAGG GTCCCCAAAA
    CCCATAGCCA ACGCACAGCC TATTGAGACC CAGAAGTCTG TGGCCAATGC CAAGTCTGCC
    GAGACCCCGA AACTAGCGGG GAAAGAAGAA CTCAAGGAGG TTAGGAATGA TGTGAACTGT
    AAGAAAGCGC ATGTCGGAGC CACCGGCAAT GAAAAAAGAC CTGAGAGCCA AGGCTCGGCC
    CCAGTTTTCA AGGAAAAGCT ACAAGATGTC CATGTGGCTG AGGGCGAGAA GCTGCTACTC
    CAGTGCCAGG TGACCTCTGA TCCTCCGGCC ACTGTCACCT GGACACTGAA TGGAAAGACA
    CTCAAGACCA CAAAATTCAT CATCCTCGCC CAAGAAGGCT CACTCTTCTC CGTCTCCATC
    GAGAAGGCAC TGCCTGAGGA CAGGGGTCTA TACAAGTGTG TAGCCAAGAA CAGCGCTGGC
    CAGGCGGAAT GTTCCTGCCA AGTCACTGTG GACGATGCTG GGACCAGCGA GAACACCAAG
    GCCCCGGAGA TGAAATCCCG GAGACCCAAG AGCTCTCTTA CGCCTGTGCT AGGAACGGAA
    AGTGATGCTA CTGTGAAAAA GAAACCTGCC TCCAAGACAC CTACAAAAGC AGCTATGCCT
    CCTCAGATCA TCCAGTTCCC TGAGGACCAG AAGGTGCGTG CTGGAGAGCC AGTGGAGCTG
    TTTGGGAAAG TGACCGGTAC CCAGCCCATC ACCTGCACGT GGATGAAGTT CCGAAAGCAG
    ATCCAGGAGA GCGAGAACAT CAGGGTGGAG AACAGTGAGA GCGGCAGCAA GCTCACCATC
    CTGGCTGCAC GGCAGGAGCA CTGTGGCTGC TACACACTGG TGGTGGAGAA CAAGCTGAGC
    AGCAGACAGG CCCAAGTCAA CCTCACGGTT GTGGATAAGC CAGACCCCCC GGCTGGCACA
    CCTTGTGCTT CTGACATACG GAGTTCCTCG CTCACCCTGT CCTGGTATGG CTCCTCGTAT
    GATGGGGGCA GTGCCGTACA GTCCTACAAT GTTGAGATCT GGGACACGGA GGACAAGGTG
    TGGACAGAGC TAGCCACGTG CCGCAGCACC TCTTTCAATG TCCAAGACCT GCTGCCTGAC
    CGCGAGTATA AATTCCGAGT ACGTGCAGTC AACGTCTACG GAACCAGCGA GCCGAGCCAG
    GAGTCTGAGC TCACCGCAGT GGGAGAGAAG CCTGAAGAGC CAAAGGATGA AGTGGAAGTG
    TCCGATGATG ACGAAAAGGA GCCTGAGGTT GATTACCGGA CGGTGACGGT GAACACTGAA
    CAGAAAGTAT CTGATGTATA TGACATTGAA GAGCGACTAG GATCTGGGAA ATTTGGACAA
    GTCTTCCGAC TTGTTGAAAA GAGAACTGGA AAAATCTGGG CAGGGAAATT TTTCAAGGCC
    TATTCCGCCA AGGAGAAAGA AAACATCCGT CAGGAGATCA GCATCATGAA CTGTCTCCAC
    CACCCCAAGC TGGTCCAGTG CGTGGATGCC TTCGAAGAAA AGGCCAACAT CGTCATGGTT
    CTGGAGATTG TGTCAGGGGG TGAGCTATTT GAGCGCATCA TTGATGAGGA CTTCGAGCTA
    ACAGAGCGGG AGTGCATCAA GTACATGAGG CAGATTTCGG AAGGGGTGGA GTACATCCAC
    AAGCAGGGCA TCGTGCATCT GGACCTCAAG CCTGAGAACA TTATGTGTGT CAACAAGACA
    GGCACCAGGA TCAAGCTCAT TGACTTCGGC CTGGCACGAA GACTCGAGAA CGCTGGGTCT
    CTGAAGGTCC TCTTTGGGAC CCCAGAGTTT GTGGCTCCTG AGGTGATCAA CTATGAGCCC
    ATCGGCTACG CCACAGACAT GTGGAGCATC GGGGTCATCT GCTATATTCT GGTCAGCGGA
    CTTTCCCCCT TCATGGGTGA CAATGATAAC GAAACTTTAG CCAATGTCAC TTCAGCTACC
    TGGGACTTCG ACGACGAGGC GTTCGATGAG ATCTCGGAAG ATGCGAAGGA CTTCATCAGT
    AACTTGCTAA AGAAAGATAT GAAAAACCGT CTGGACTGCA CCCAGTGCCT TCAGCACCCA
    TGGCTGATGA AAGACACCAA GAACATGGAG GCCAAGAAGC TGTCCAAGGA CCGGATGAAG
    AAATACATGG CAAGAAGGAA GTGGCAGAAA ACAGGCAATG CTGTGAGAGC CATTGGGAGA
    CTGTCCTCTA TGGCAATGAT CTCAGGGCTC AGTGGCAGGA AATCTTCAAC AGGGTCACCA
    ACCAGCCCGA TCAATGCAGA GAAGCTAGAG TCTGAAGAAG ATGTCTCCCA GGCCTTCCTC
    GAGGCTGTTG CTGAGGAAAA GCCCCATGTA AAGCCCTACT TCTCTAAGAC CATTCGTGAC
    CTGGAGGTTG TGGAGGGAAG CGCTGCTAGA TTTGACTGCA AGATCGAAGG ATACCCAGAC
    CCTGAGGTTG TCTGGTTCAA GGATGACCAG TCAATCAGGG AGTCCCGCCA CTTCCAGATA
    GACTACGATG AAGACGGGAA CTGCTCGCTA ATTATCAGTG ATGTCTGTGG GGATGATGAC
    GCCAAGTACA CCTGCAAGGC TGTCAACAGC CTCGGAGAAG CCACCTGCAC CGCGGAGCTC
    ATCGTGGAGA CCATGGAGGA AGGGGAAGGG GAGGAGGGGG GGGAGGAGGA GGAGGAGGAG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001099344.2
    CDS41..5926
    Translation

    Target ORF information:

    RefSeq Version NM_001105874.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus myosin light chain kinase (Mylk), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001105874.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCCTGGCC AGTTACCTGG GAAGACCATG GGGGACGTGA AACTGTTTGC CTCGTCGCAC 
    ATGTCCAAAA CCTCCCACAG TGTGGATCCC TCGAGGGTCA GCTCTCTGCC TCTCACCGAG
    GCCCCTGCTT TCATTCTGCC TCCTCGGAAC CTCTGCGTCA AAGAAGGAGC CACCGCCAAG
    TTTGAAGGGA GGGTCCGGGG CCACCCAGAG CCCCAGGTGA CATGGCACAG AAATGGGCAA
    ACCATCACCA ATGGCGGCCG CTTCCTGCTG GACGGTGGGG TGCGGGGGAC TTTCAGCCTC
    GTCATCCGTG GTGTCCGTGA GGAGGACAAG GGCAAGTACA CCTGTGAAGC TAGCAACGGC
    AGTGGCGCCC GCCAGGTGAC CGTGGAGCTG ACTGTGGAAG GGGATCTTAT GAAGAAGCAT
    GGTCAGCCCG TTCTTTCTAA AGCCTCAGGA TTTCCAGGGG AGACTCGTCC TAGCATCTGG
    GGTGAGTGCC CGCCAAAGTT TGCCACCAAG CTGGGCAGAG CTGTGGTCAA AGAAGGGCAG
    ATGGGGCGAT TTTCCTGCAA GATCACTGGC CGGCCTCCAC CACAGGTCAC CTGGCTCAAG
    GGAAATGTCC CGTTGCAGCC AAGTGCCCGA GTGTCTATGT CAGAGAAGAA TGGCATGCAG
    ATCCTGGAAA TCCACGAGGT CACTCAGGAC GACATGGGCA CGTACACATG CATGGTGGTG
    AATGGGTCAG GGAAAGCCTC CATGTCAGCG GAGCTCTCCA TCCCAGGTTT GGACAATGCC
    ACCAGGTTGT CAAGAGGAAC CAAGGACCCC AGTCCAGACA TCAGGAAGGA GGTGACCAAT
    GGAATCTCCA AGGACCCAGA GACTGTGGCT GAAAGTAAGA ACTGTTCAAG TCCCCAGAAG
    AGCAACTTCT TAGCCCAGGC CACCAACAGT CACCTCAAGT CCCTGCAGCA GCCAAAGCTG
    AAGCCGTGTG AAGATTCCCC CAGAAAGGTC CTGCAGTCCT CCGTCCTGCA GAAGACCTCC
    AGCACCATTG TTCTGCAGGC CTCAAAAGTC CAGCCAGAAG TGAGAGCAGC GGGCATAGGG
    GCCTCCGGTC CTGGGGAAGA GAGGAAGAGT CTGACTGCCC CTCGGCAAGC CACACCCCCT
    ACCAGACAGT GTGGCCTGGG AGGCTCAGTT GGGAACAAAT TTATCACGGG GAACATCCCC
    ATAGAGAACC AAAGGGAGTC AAGATTTCCC AAGTTTGAGA GTCAGCCCCA AAGCCAAGAA
    GTCACTGAAG GTCAAACAGT CAAGTTCACC TGTGAGGTTT CAGGGATACC AAAGCCTGAA
    GTGGGTTGGT TCCTGGAAGG CATCCCCGTG AGGAGACAAG AAGGCATCGT TGAGATTTAT
    GAAGACGGTA CATCCCATTA CCTGTGTCTG CCGAGAGCCG GGACGAGAGA CAGCGGGAGA
    TACAGCTGCA CAGCATCCAA TACCCTTGGC CAAGTGTCCT GCAGTTGGAC GCTCCGGGTG
    GACCGGCCAA GCTTGACGCA GACAGCCCCT TCCTTCTCTA GTGTTCTGAA GGACAGTGTT
    GTCATTGAAG GCCAGGATTT TGTGCTACGG TGCTCTGTAC GGGGGACCCC AGCACCCCAG
    GTCACTTGGC TGCTCAATGG GCAGCCCATC CAGTTTGCTC GATCCATCTG TGAGGCTGGC
    GAGGCCGAGC TCCACATCCA GGATGCCCTG CCCGAGGACC GTGGCACCTA CACCTGCCTG
    GCTGAGAATG CCTTGGGGCA GGTGTCCTGC AGTGCCACGG TCACGGTCCA AGAAAAGAAA
    GGCGAAGGAG AGAGGGCGCA CCGTCTGCCC CCAGCTCCCA GCAAGCTCTT CGCTCCCATC
    TTCCTGCAGG GTCTCTCTGA CCTCAAAGTC ATGGATGGAA GCCAGGTCAC TATGACAGTC
    CAGGTGTCAG GAAACCCACC TCCAGAAGTC ATATGGCTTC ACGACGGGAA CGAGATCCAG
    GAGTCAGAGG ACTTCCACTT CGAGCAGAAA GGTGGCTGGC ACAGCCTATG TATCCAGGAA
    GTGTTCCCGG AGGACACGGG CACATACACC TGTGAGGCCT GGAACAGCGC TGGGGAGGTC
    CGCACCCAGG CCATACTCAC CGTGCAAGAG CCCCATGATG GCACCCAGCC CTGGTTCATC
    AGCAAGCCTC GTTCGGTGAC AGCCTCCCTG GGCCAGAGTG TCCTCATCTC CTGCGCCATA
    GCTGGAGACC CCTTTCCCAC TGTGCACTGG CTCCGAGATG GCAAAGCCCT CTCCAGGGAC
    CATGGCCACT TTGAGCTGCT CCAGAATGAG GACGTGTTCA CCCTGGTTCT AAAGAACGTG
    CAGCCCTGGC ATGCCGGCCA GTATGAGATC CTGCTCAAGA ACCGGGTTGG CGAGTGCAGT
    TGCCAAGTGT CACTGATGCT GCACAAAGGT CCTTCCAGAG TCCCACCACC ACGGGGGCGG
    GAGCCTGCCA GCTGTGAGGG CCTCTGTGGT GGAGGAGTTG GTGCTCATGG TGATGCTGAC
    CGTGATGGGA CCCTGAGGCC CTGCTGGCCA GCAAGAGGGC AGGGTTGGCC TGAGGAGGAA
    GACGGAGAAG ACGTGAGGGG GCTGTTGAAG AGACGGGTGG AGACCAGGCT GCACACAGAA
    GAGGCCATCC GCCAGCAGGA GGTGGGGCAG CTGGATTTTC GAGACCTCCT GGGAAAGAAG
    GTGAGCACAA AGACCGTATC AGAAGACGAC CTGAAGGACA TCCCAGCCGA GCAGATGGAT
    TTCCGCGCCA ACCTTCAGCG GCAAGTGAAG CCAAAGACTG TGTCTGAGGA AGAGAGGAAG
    GTGCATAGCC CCCAGCAGGT TGACTTCCGG TCTGTCCTAG CCAAGAAGGG GACCCCTAAG
    ACCCCTGTCC CTGAGAAGGT GCCACCTCAA AAAGCTGCCA CCCCAGACTT TCGCTCAGTG
    CTAGGTAGCA AGAAGAAGTC GCCTGCGGAG AATGGGGGCA ACACTGCTGA GGTCTTGAAT
    GTCAAGGCTG GGGAAAGCCC CACGCCTGCA GGCAATGCAC AGCCAGTCGG GGCCCTGAAA
    CCCATAGGCA ACGCCAAGCC TGCTGAGACC CTGAAGTCCG TGGGCAATGC CAAACCAGCA
    GAGACCCTGA AGCCCGTGGG GAATGCCAAG CCAGCAGAGA CCCTGAAACC CGTAGGTAAT
    GTCAAGCCTG CCGAGACCCT GAAGCCCATA GCCAACGCAC AGCCTTCAGG GTCCCCAAAA
    CCCATAGCCA ACGCACAGCC TATTGAGACC CAGAAGTCTG TGGCCAATGC CAAGTCTGCC
    GAGACCCCGA AACTAGCGGG GAAAGAAGAA CTCAAGGAGG TTAGGAATGA TGTGAACTGT
    AAGAAAGCGC ATGTCGGAGC CACCGGCAAT GAAAAAAGAC CTGAGAGCCA AGGCTCGGCC
    CCAGTTTTCA AGGAAAAGCT ACAAGATGTC CATGTGGCTG AGGGCGAGAA GCTGCTACTC
    CAGTGCCAGG TGACCTCTGA TCCTCCGGCC ACTGTCACCT GGACACTGAA TGGAAAGACA
    CTCAAGACCA CAAAATTCAT CATCCTCGCC CAAGAAGGCT CACTCTTCTC CGTCTCCATC
    GAGAAGGCAC TGCCTGAGGA CAGGGGTCTA TACAAGTGTG TAGCCAAGAA CAGCGCTGGC
    CAGGCGGAAT GTTCCTGCCA AGTCACTGTG GACGATGCTG GGACCAGCGA GAACACCAAG
    GCCCCGGAGA TGAAATCCCG GAGACCCAAG AGCTCTCTTA CGCCTGTGCT AGGAACGGAA
    AGTGATGCTA CTGTGAAAAA GAAACCTGCC TCCAAGACAC CTACAAAAGC AGCTATGCCT
    CCTCAGATCA TCCAGTTCCC TGAGGACCAG AAGGTGCGTG CTGGAGAGCC AGTGGAGCTG
    TTTGGGAAAG TGACCGGTAC CCAGCCCATC ACCTGCACGT GGATGAAGTT CCGAAAGCAG
    ATCCAGGAGA GCGAGAACAT CAGGGTGGAG AACAGTGAGA GCGGCAGCAA GCTCACCATC
    CTGGCTGCAC GGCAGGAGCA CTGTGGCTGC TACACACTGG TGGTGGAGAA CAAGCTGAGC
    AGCAGACAGG CCCAAGTCAA CCTCACGGTT GTGGATAAGC CAGACCCCCC GGCTGGCACA
    CCTTGTGCTT CTGACATACG GAGTTCCTCG CTCACCCTGT CCTGGTATGG CTCCTCGTAT
    GATGGGGGCA GTGCCGTACA GTCCTACAAT GTTGAGATCT GGGACACGGA GGACAAGGTG
    TGGACAGAGC TAGCCACGTG CCGCAGCACC TCTTTCAATG TCCAAGACCT GCTGCCTGAC
    CGCGAGTATA AATTCCGAGT ACGTGCAGTC AACGTCTACG GAACCAGCGA GCCGAGCCAG
    GAGTCTGAGC TCACCGCAGT GGGAGAGAAG CCTGAAGAGC CAAAGGATGA AGTGGAAGTG
    TCCGATGATG ACGAAAAGGA GCCTGAGGTT GATTACCGGA CGGTGACGGT GAACACTGAA
    CAGAAAGTAT CTGATGTATA TGACATTGAA GAGCGACTAG GATCTGGGAA ATTTGGACAA
    GTCTTCCGAC TTGTTGAAAA GAGAACTGGA AAAATCTGGG CAGGGAAATT TTTCAAGGCC
    TATTCCGCCA AGGAGAAAGA AAACATCCGT CAGGAGATCA GCATCATGAA CTGTCTCCAC
    CACCCCAAGC TGGTCCAGTG CGTGGATGCC TTCGAAGAAA AGGCCAACAT CGTCATGGTT
    CTGGAGATTG TGTCAGGGGG TGAGCTATTT GAGCGCATCA TTGATGAGGA CTTCGAGCTA
    ACAGAGCGGG AGTGCATCAA GTACATGAGG CAGATTTCGG AAGGGGTGGA GTACATCCAC
    AAGCAGGGCA TCGTGCATCT GGACCTCAAG CCTGAGAACA TTATGTGTGT CAACAAGACA
    GGCACCAGGA TCAAGCTCAT TGACTTCGGC CTGGCACGAA GACTCGAGAA CGCTGGGTCT
    CTGAAGGTCC TCTTTGGGAC CCCAGAGTTT GTGGCTCCTG AGGTGATCAA CTATGAGCCC
    ATCGGCTACG CCACAGACAT GTGGAGCATC GGGGTCATCT GCTATATTCT GGTCAGCGGA
    CTTTCCCCCT TCATGGGTGA CAATGATAAC GAAACTTTAG CCAATGTCAC TTCAGCTACC
    TGGGACTTCG ACGACGAGGC GTTCGATGAG ATCTCGGAAG ATGCGAAGGA CTTCATCAGT
    AACTTGCTAA AGAAAGATAT GAAAAACCGT CTGGACTGCA CCCAGTGCCT TCAGCACCCA
    TGGCTGATGA AAGACACCAA GAACATGGAG GCCAAGAAGC TGTCCAAGGA CCGGATGAAG
    AAATACATGG CAAGAAGGAA GTGGCAGAAA ACAGGCAATG CTGTGAGAGC CATTGGGAGA
    CTGTCCTCTA TGGCAATGAT CTCAGGGCTC AGTGGCAGGA AATCTTCAAC AGGGTCACCA
    ACCAGCCCGA TCAATGCAGA GAAGCTAGAG TCTGAAGAAG ATGTCTCCCA GGCCTTCCTC
    GAGGCTGTTG CTGAGGAAAA GCCCCATGTA AAGCCCTACT TCTCTAAGAC CATTCGTGAC
    CTGGAGGTTG TGGAGGGAAG CGCTGCTAGA TTTGACTGCA AGATCGAAGG ATACCCAGAC
    CCTGAGGTTG TCTGGTTCAA GGATGACCAG TCAATCAGGG AGTCCCGCCA CTTCCAGATA
    GACTACGATG AAGACGGGAA CTGCTCGCTA ATTATCAGTG ATGTCTGTGG GGATGATGAC
    GCCAAGTACA CCTGCAAGGC TGTCAACAGC CTCGGAGAAG CCACCTGCAC CGCGGAGCTC
    ATCGTGGAGA CCATGGAGGA AGGGGAAGGG GAGGAGGGGG GGGAGGAGGA GGAGGAGGAG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa34329
    Clone ID Related Accession (Same CDS sequence) XM_017597921.1 , XM_006248434.3 , XM_006248433.3
    Accession Version XM_017597921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
    CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG
    AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA
    GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG
    CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC
    AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG
    CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA
    GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC
    AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT
    GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC
    CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG
    GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA
    GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC
    CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG
    GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC
    AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG
    GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA
    GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG
    AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA
    GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT
    CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC
    ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC
    GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT
    CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT
    GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC
    CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA
    CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC
    ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC
    CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC
    TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG
    CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA
    GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA
    GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG
    AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC
    ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA
    GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC
    CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC
    TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT
    GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG
    ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA
    GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT
    GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG
    CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG
    AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG
    CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC
    GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG
    AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC
    CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT
    CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG
    GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT
    GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG
    ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC
    AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC
    ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG
    ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA
    GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC
    AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT
    GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG
    GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC
    GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT
    CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT
    GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC
    AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT
    GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC
    CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC
    ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG
    GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC
    TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG
    GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC
    TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC
    AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC
    ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA
    GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG
    AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG
    GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT
    GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT
    GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC
    CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT
    GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA
    TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG
    AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC
    AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC
    GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG
    TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC
    ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT
    AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT
    GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC
    CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG
    GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG
    AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG
    CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA
    GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT
    GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT
    AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC
    CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG
    CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC
    AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA
    GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453410.1
    CDS5..5863
    Translation

    Target ORF information:

    RefSeq Version XM_017597921.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017597921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
    CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG
    AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA
    GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG
    CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC
    AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG
    CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA
    GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC
    AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT
    GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC
    CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG
    GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA
    GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC
    CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG
    GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC
    AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG
    GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA
    GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG
    AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA
    GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT
    CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC
    ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC
    GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT
    CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT
    GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC
    CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA
    CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC
    ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC
    CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC
    TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG
    CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA
    GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA
    GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG
    AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC
    ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA
    GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC
    CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC
    TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT
    GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG
    ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA
    GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT
    GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG
    CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG
    AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG
    CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC
    GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG
    AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC
    CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT
    CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG
    GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT
    GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG
    ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC
    AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC
    ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG
    ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA
    GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC
    AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT
    GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG
    GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC
    GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT
    CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT
    GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC
    AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT
    GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC
    CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC
    ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG
    GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC
    TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG
    GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC
    TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC
    AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC
    ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA
    GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG
    AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG
    GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT
    GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT
    GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC
    CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT
    GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA
    TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG
    AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC
    AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC
    GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG
    TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC
    ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT
    AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT
    GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC
    CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG
    GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG
    AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG
    CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA
    GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT
    GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT
    AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC
    CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG
    CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC
    AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA
    GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa34329
    Clone ID Related Accession (Same CDS sequence) XM_017597921.1 , XM_006248434.3 , XM_006248433.3
    Accession Version XM_006248434.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248434.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
    CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG
    AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA
    GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG
    CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC
    AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG
    CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA
    GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC
    AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT
    GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC
    CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG
    GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA
    GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC
    CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG
    GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC
    AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG
    GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA
    GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG
    AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA
    GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT
    CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC
    ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC
    GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT
    CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT
    GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC
    CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA
    CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC
    ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC
    CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC
    TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG
    CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA
    GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA
    GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG
    AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC
    ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA
    GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC
    CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC
    TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT
    GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG
    ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA
    GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT
    GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG
    CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG
    AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG
    CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC
    GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG
    AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC
    CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT
    CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG
    GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT
    GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG
    ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC
    AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC
    ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG
    ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA
    GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC
    AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT
    GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG
    GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC
    GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT
    CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT
    GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC
    AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT
    GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC
    CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC
    ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG
    GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC
    TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG
    GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC
    TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC
    AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC
    ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA
    GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG
    AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG
    GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT
    GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT
    GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC
    CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT
    GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA
    TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG
    AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC
    AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC
    GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG
    TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC
    ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT
    AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT
    GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC
    CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG
    GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG
    AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG
    CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA
    GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT
    GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT
    AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC
    CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG
    CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC
    AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA
    GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006248496.1
    CDS160..6018
    Translation

    Target ORF information:

    RefSeq Version XM_006248434.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006248434.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
    CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG
    AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA
    GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG
    CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC
    AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG
    CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA
    GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC
    AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT
    GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC
    CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG
    GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA
    GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC
    CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG
    GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC
    AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG
    GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA
    GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG
    AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA
    GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT
    CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC
    ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC
    GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT
    CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT
    GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC
    CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA
    CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC
    ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC
    CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC
    TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG
    CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA
    GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA
    GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG
    AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC
    ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA
    GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC
    CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC
    TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT
    GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG
    ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA
    GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT
    GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG
    CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG
    AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG
    CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC
    GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG
    AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC
    CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT
    CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG
    GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT
    GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG
    ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC
    AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC
    ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG
    ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA
    GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC
    AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT
    GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG
    GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC
    GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT
    CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT
    GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC
    AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT
    GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC
    CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC
    ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG
    GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC
    TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG
    GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC
    TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC
    AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC
    ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA
    GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG
    AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG
    GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT
    GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT
    GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC
    CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT
    GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA
    TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG
    AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC
    AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC
    GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG
    TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC
    ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT
    AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT
    GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC
    CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG
    GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG
    AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG
    CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA
    GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT
    GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT
    AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC
    CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG
    CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC
    AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA
    GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa34329
    Clone ID Related Accession (Same CDS sequence) XM_017597921.1 , XM_006248434.3 , XM_006248433.3
    Accession Version XM_006248433.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248433.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
    CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG
    AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA
    GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG
    CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC
    AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG
    CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA
    GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC
    AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT
    GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC
    CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG
    GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA
    GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC
    CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG
    GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC
    AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG
    GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA
    GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG
    AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA
    GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT
    CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC
    ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC
    GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT
    CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT
    GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC
    CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA
    CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC
    ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC
    CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC
    TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG
    CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA
    GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA
    GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG
    AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC
    ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA
    GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC
    CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC
    TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT
    GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG
    ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA
    GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT
    GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG
    CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG
    AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG
    CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC
    GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG
    AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC
    CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT
    CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG
    GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT
    GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG
    ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC
    AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC
    ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG
    ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA
    GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC
    AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT
    GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG
    GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC
    GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT
    CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT
    GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC
    AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT
    GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC
    CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC
    ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG
    GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC
    TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG
    GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC
    TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC
    AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC
    ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA
    GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG
    AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG
    GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT
    GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT
    GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC
    CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT
    GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA
    TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG
    AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC
    AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC
    GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG
    TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC
    ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT
    AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT
    GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC
    CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG
    GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG
    AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG
    CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA
    GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT
    GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT
    AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC
    CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG
    CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC
    AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA
    GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006248495.1
    CDS369..6227
    Translation

    Target ORF information:

    RefSeq Version XM_006248433.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006248433.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
    CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG
    AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA
    GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG
    CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC
    AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG
    CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA
    GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC
    AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT
    GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC
    CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG
    GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA
    GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC
    CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG
    GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC
    AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG
    GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA
    GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG
    AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA
    GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT
    CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC
    ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC
    GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT
    CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT
    GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC
    CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA
    CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC
    ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC
    CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC
    TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG
    CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA
    GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA
    GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG
    AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC
    ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA
    GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC
    CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC
    TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT
    GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG
    ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA
    GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT
    GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG
    CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG
    AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG
    CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC
    GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG
    AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC
    CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT
    CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG
    GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT
    GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG
    ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC
    AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC
    ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG
    ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA
    GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC
    AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT
    GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG
    GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC
    GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT
    CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT
    GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC
    AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT
    GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC
    CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC
    ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG
    GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC
    TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG
    GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC
    TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC
    AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC
    ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA
    GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG
    AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG
    GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT
    GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT
    GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC
    CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT
    GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA
    TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG
    AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC
    AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC
    GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG
    TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC
    ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT
    AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT
    GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC
    CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG
    GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG
    AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG
    CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA
    GAGTCTGAAG AAGATGTCTC CCAGGCCTTC CTCGAGGCTG TTGCTGAGGA AAAGCCCCAT
    GTAAAGCCCT ACTTCTCTAA GACCATTCGT GACCTGGAGG TTGTGGAGGG AAGCGCTGCT
    AGATTTGACT GCAAGATCGA AGGATACCCA GACCCTGAGG TTGTCTGGTT CAAGGATGAC
    CAGTCAATCA GGGAGTCCCG CCACTTCCAG ATAGACTACG ATGAAGACGG GAACTGCTCG
    CTAATTATCA GTGATGTCTG TGGGGATGAT GACGCCAAGT ACACCTGCAA GGCTGTCAAC
    AGCCTCGGAG AAGCCACCTG CACCGCGGAG CTCATCGTGG AGACCATGGA GGAAGGGGAA
    GGGGAGGAGG GGGGGGAGGA GGAGGAGGAG GAGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa44660
    Clone ID Related Accession (Same CDS sequence) XM_008768711.2
    Accession Version XM_008768711.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
    CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG
    AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA
    GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG
    CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC
    AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG
    CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA
    GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC
    AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT
    GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC
    CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG
    GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA
    GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC
    CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG
    GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC
    AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG
    GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA
    GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG
    AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA
    GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT
    CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC
    ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC
    GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT
    CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT
    GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC
    CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA
    CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC
    ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC
    CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC
    TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG
    CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA
    GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA
    GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG
    AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC
    ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA
    GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC
    CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC
    TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT
    GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG
    ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA
    GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT
    GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG
    CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG
    AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG
    CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC
    GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG
    AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC
    CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT
    CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG
    GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT
    GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG
    ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC
    AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC
    ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG
    ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA
    GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC
    AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT
    GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG
    GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC
    GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT
    CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT
    GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC
    AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT
    GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC
    CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC
    ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG
    GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC
    TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG
    GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC
    TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC
    AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC
    ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA
    GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG
    AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG
    GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT
    GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT
    GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC
    CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT
    GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA
    TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG
    AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC
    AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC
    GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG
    TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC
    ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT
    AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT
    GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC
    CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG
    GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG
    AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG
    CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA
    GAGTCTGAAG ATGTCTCCCA GGCCTTCCTC GAGGCTGTTG CTGAGGAAAA GCCCCATGTA
    AAGCCCTACT TCTCTAAGAC CATTCGTGAC CTGGAGGTTG TGGAGGGAAG CGCTGCTAGA
    TTTGACTGCA AGATCGAAGG ATACCCAGAC CCTGAGGTTG TCTGGTTCAA GGATGACCAG
    TCAATCAGGG AGTCCCGCCA CTTCCAGATA GACTACGATG AAGACGGGAA CTGCTCGCTA
    ATTATCAGTG ATGTCTGTGG GGATGATGAC GCCAAGTACA CCTGCAAGGC TGTCAACAGC
    CTCGGAGAAG CCACCTGCAC CGCGGAGCTC ATCGTGGAGA CCATGGAGGA AGGGGAAGGG
    GAGGAGGGGG GGGAGGAGGA GGAGGAGGAG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008766933.1
    CDS370..6225
    Translation

    Target ORF information:

    RefSeq Version XM_008768711.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus myosin light chain kinase (Mylk), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008768711.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGGGACG TGAAACTGTT TGCCTCGTCG CACATGTCCA AAACCTCCCA CAGTGTGGAT 
    CCCTCGAGGG TCAGCTCTCT GCCTCTCACC GAGGCCCCTG CTTTCATTCT GCCTCCTCGG
    AACCTCTGCG TCAAAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGCCACCCA
    GAGCCCCAGG TGACATGGCA CAGAAATGGG CAAACCATCA CCAATGGCGG CCGCTTCCTG
    CTGGACGGTG GGGTGCGGGG GACTTTCAGC CTCGTCATCC GTGGTGTCCG TGAGGAGGAC
    AAGGGCAAGT ACACCTGTGA AGCTAGCAAC GGCAGTGGCG CCCGCCAGGT GACCGTGGAG
    CTGACTGTGG AAGGGGATCT TATGAAGAAG CATGGTCAGC CCGTTCTTTC TAAAGCCTCA
    GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCGCCAAA GTTTGCCACC
    AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTTTCCTG CAAGATCACT
    GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC
    CGAGTGTCTA TGTCAGAGAA GAATGGCATG CAGATCCTGG AAATCCACGA GGTCACTCAG
    GACGACATGG GCACGTACAC ATGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCA
    GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCACCAGGT TGTCAAGAGG AACCAAGGAC
    CCCAGTCCAG ACATCAGGAA GGAGGTGACC AATGGAATCT CCAAGGACCC AGAGACTGTG
    GCTGAAAGTA AGAACTGTTC AAGTCCCCAG AAGAGCAACT TCTTAGCCCA GGCCACCAAC
    AGTCACCTCA AGTCCCTGCA GCAGCCAAAG CTGAAGCCGT GTGAAGATTC CCCCAGAAAG
    GTCCTGCAGT CCTCCGTCCT GCAGAAGACC TCCAGCACCA TTGTTCTGCA GGCCTCAAAA
    GTCCAGCCAG AAGTGAGAGC AGCGGGCATA GGGGCCTCCG GTCCTGGGGA AGAGAGGAAG
    AGTCTGACTG CCCCTCGGCA AGCCACACCC CCTACCAGAC AGTGTGGCCT GGGAGGCTCA
    GTTGGGAACA AATTTATCAC GGGGAACATC CCCATAGAGA ACCAAAGGGA GTCAAGATTT
    CCCAAGTTTG AGAGTCAGCC CCAAAGCCAA GAAGTCACTG AAGGTCAAAC AGTCAAGTTC
    ACCTGTGAGG TTTCAGGGAT ACCAAAGCCT GAAGTGGGTT GGTTCCTGGA AGGCATCCCC
    GTGAGGAGAC AAGAAGGCAT CGTTGAGATT TATGAAGACG GTACATCCCA TTACCTGTGT
    CTGCCGAGAG CCGGGACGAG AGACAGCGGG AGATACAGCT GCACAGCATC CAATACCCTT
    GGCCAAGTGT CCTGCAGTTG GACGCTCCGG GTGGACCGGC CAAGCTTGAC GCAGACAGCC
    CCTTCCTTCT CTAGTGTTCT GAAGGACAGT GTTGTCATTG AAGGCCAGGA TTTTGTGCTA
    CGGTGCTCTG TACGGGGGAC CCCAGCACCC CAGGTCACTT GGCTGCTCAA TGGGCAGCCC
    ATCCAGTTTG CTCGATCCAT CTGTGAGGCT GGCGAGGCCG AGCTCCACAT CCAGGATGCC
    CTGCCCGAGG ACCGTGGCAC CTACACCTGC CTGGCTGAGA ATGCCTTGGG GCAGGTGTCC
    TGCAGTGCCA CGGTCACGGT CCAAGAAAAG AAAGGCGAAG GAGAGAGGGC GCACCGTCTG
    CCCCCAGCTC CCAGCAAGCT CTTCGCTCCC ATCTTCCTGC AGGGTCTCTC TGACCTCAAA
    GTCATGGATG GAAGCCAGGT CACTATGACA GTCCAGGTGT CAGGAAACCC ACCTCCAGAA
    GTCATATGGC TTCACGACGG GAACGAGATC CAGGAGTCAG AGGACTTCCA CTTCGAGCAG
    AAAGGTGGCT GGCACAGCCT ATGTATCCAG GAAGTGTTCC CGGAGGACAC GGGCACATAC
    ACCTGTGAGG CCTGGAACAG CGCTGGGGAG GTCCGCACCC AGGCCATACT CACCGTGCAA
    GAGCCCCATG ATGGCACCCA GCCCTGGTTC ATCAGCAAGC CTCGTTCGGT GACAGCCTCC
    CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC ATAGCTGGAG ACCCCTTTCC CACTGTGCAC
    TGGCTCCGAG ATGGCAAAGC CCTCTCCAGG GACCATGGCC ACTTTGAGCT GCTCCAGAAT
    GAGGACGTGT TCACCCTGGT TCTAAAGAAC GTGCAGCCCT GGCATGCCGG CCAGTATGAG
    ATCCTGCTCA AGAACCGGGT TGGCGAGTGC AGTTGCCAAG TGTCACTGAT GCTGCACAAA
    GGTCCTTCCA GAGTCCCACC ACCACGGGGG CGGGAGCCTG CCAGCTGTGA GGGCCTCTGT
    GGTGGAGGAG TTGGTGCTCA TGGTGATGCT GACCGTGATG GGACCCTGAG GCCCTGCTGG
    CCAGCAAGAG GGCAGGGTTG GCCTGAGGAG GAAGACGGAG AAGACGTGAG GGGGCTGTTG
    AAGAGACGGG TGGAGACCAG GCTGCACACA GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG
    CAGCTGGATT TTCGAGACCT CCTGGGAAAG AAGGTGAGCA CAAAGACCGT ATCAGAAGAC
    GACCTGAAGG ACATCCCAGC CGAGCAGATG GATTTCCGCG CCAACCTTCA GCGGCAAGTG
    AAGCCAAAGA CTGTGTCTGA GGAAGAGAGG AAGGTGCATA GCCCCCAGCA GGTTGACTTC
    CGGTCTGTCC TAGCCAAGAA GGGGACCCCT AAGACCCCTG TCCCTGAGAA GGTGCCACCT
    CAAAAAGCTG CCACCCCAGA CTTTCGCTCA GTGCTAGGTA GCAAGAAGAA GTCGCCTGCG
    GAGAATGGGG GCAACACTGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACGCCT
    GCAGGCAATG CACAGCCAGT CGGGGCCCTG AAACCCATAG GCAACGCCAA GCCTGCTGAG
    ACCCTGAAGT CCGTGGGCAA TGCCAAACCA GCAGAGACCC TGAAGCCCGT GGGGAATGCC
    AAGCCAGCAG AGACCCTGAA ACCCGTAGGT AATGTCAAGC CTGCCGAGAC CCTGAAGCCC
    ATAGCCAACG CACAGCCTTC AGGGTCCCCA AAACCCATAG CCAACGCACA GCCTATTGAG
    ACCCAGAAGT CTGTGGCCAA TGCCAAGTCT GCCGAGACCC CGAAACTAGC GGGGAAAGAA
    GAACTCAAGG AGGTTAGGAA TGATGTGAAC TGTAAGAAAG CGCATGTCGG AGCCACCGGC
    AATGAAAAAA GACCTGAGAG CCAAGGCTCG GCCCCAGTTT TCAAGGAAAA GCTACAAGAT
    GTCCATGTGG CTGAGGGCGA GAAGCTGCTA CTCCAGTGCC AGGTGACCTC TGATCCTCCG
    GCCACTGTCA CCTGGACACT GAATGGAAAG ACACTCAAGA CCACAAAATT CATCATCCTC
    GCCCAAGAAG GCTCACTCTT CTCCGTCTCC ATCGAGAAGG CACTGCCTGA GGACAGGGGT
    CTATACAAGT GTGTAGCCAA GAACAGCGCT GGCCAGGCGG AATGTTCCTG CCAAGTCACT
    GTGGACGATG CTGGGACCAG CGAGAACACC AAGGCCCCGG AGATGAAATC CCGGAGACCC
    AAGAGCTCTC TTACGCCTGT GCTAGGAACG GAAAGTGATG CTACTGTGAA AAAGAAACCT
    GCCTCCAAGA CACCTACAAA AGCAGCTATG CCTCCTCAGA TCATCCAGTT CCCTGAGGAC
    CAGAAGGTGC GTGCTGGAGA GCCAGTGGAG CTGTTTGGGA AAGTGACCGG TACCCAGCCC
    ATCACCTGCA CGTGGATGAA GTTCCGAAAG CAGATCCAGG AGAGCGAGAA CATCAGGGTG
    GAGAACAGTG AGAGCGGCAG CAAGCTCACC ATCCTGGCTG CACGGCAGGA GCACTGTGGC
    TGCTACACAC TGGTGGTGGA GAACAAGCTG AGCAGCAGAC AGGCCCAAGT CAACCTCACG
    GTTGTGGATA AGCCAGACCC CCCGGCTGGC ACACCTTGTG CTTCTGACAT ACGGAGTTCC
    TCGCTCACCC TGTCCTGGTA TGGCTCCTCG TATGATGGGG GCAGTGCCGT ACAGTCCTAC
    AATGTTGAGA TCTGGGACAC GGAGGACAAG GTGTGGACAG AGCTAGCCAC GTGCCGCAGC
    ACCTCTTTCA ATGTCCAAGA CCTGCTGCCT GACCGCGAGT ATAAATTCCG AGTACGTGCA
    GTCAACGTCT ACGGAACCAG CGAGCCGAGC CAGGAGTCTG AGCTCACCGC AGTGGGAGAG
    AAGCCTGAAG AGCCAAAGGA TGAAGTGGAA GTGTCCGATG ATGACGAAAA GGAGCCTGAG
    GTTGATTACC GGACGGTGAC GGTGAACACT GAACAGAAAG TATCTGATGT ATATGACATT
    GAAGAGCGAC TAGGATCTGG GAAATTTGGA CAAGTCTTCC GACTTGTTGA AAAGAGAACT
    GGAAAAATCT GGGCAGGGAA ATTTTTCAAG GCCTATTCCG CCAAGGAGAA AGAAAACATC
    CGTCAGGAGA TCAGCATCAT GAACTGTCTC CACCACCCCA AGCTGGTCCA GTGCGTGGAT
    GCCTTCGAAG AAAAGGCCAA CATCGTCATG GTTCTGGAGA TTGTGTCAGG GGGTGAGCTA
    TTTGAGCGCA TCATTGATGA GGACTTCGAG CTAACAGAGC GGGAGTGCAT CAAGTACATG
    AGGCAGATTT CGGAAGGGGT GGAGTACATC CACAAGCAGG GCATCGTGCA TCTGGACCTC
    AAGCCTGAGA ACATTATGTG TGTCAACAAG ACAGGCACCA GGATCAAGCT CATTGACTTC
    GGCCTGGCAC GAAGACTCGA GAACGCTGGG TCTCTGAAGG TCCTCTTTGG GACCCCAGAG
    TTTGTGGCTC CTGAGGTGAT CAACTATGAG CCCATCGGCT ACGCCACAGA CATGTGGAGC
    ATCGGGGTCA TCTGCTATAT TCTGGTCAGC GGACTTTCCC CCTTCATGGG TGACAATGAT
    AACGAAACTT TAGCCAATGT CACTTCAGCT ACCTGGGACT TCGACGACGA GGCGTTCGAT
    GAGATCTCGG AAGATGCGAA GGACTTCATC AGTAACTTGC TAAAGAAAGA TATGAAAAAC
    CGTCTGGACT GCACCCAGTG CCTTCAGCAC CCATGGCTGA TGAAAGACAC CAAGAACATG
    GAGGCCAAGA AGCTGTCCAA GGACCGGATG AAGAAATACA TGGCAAGAAG GAAGTGGCAG
    AAAACAGGCA ATGCTGTGAG AGCCATTGGG AGACTGTCCT CTATGGCAAT GATCTCAGGG
    CTCAGTGGCA GGAAATCTTC AACAGGGTCA CCAACCAGCC CGATCAATGC AGAGAAGCTA
    GAGTCTGAAG ATGTCTCCCA GGCCTTCCTC GAGGCTGTTG CTGAGGAAAA GCCCCATGTA
    AAGCCCTACT TCTCTAAGAC CATTCGTGAC CTGGAGGTTG TGGAGGGAAG CGCTGCTAGA
    TTTGACTGCA AGATCGAAGG ATACCCAGAC CCTGAGGTTG TCTGGTTCAA GGATGACCAG
    TCAATCAGGG AGTCCCGCCA CTTCCAGATA GACTACGATG AAGACGGGAA CTGCTCGCTA
    ATTATCAGTG ATGTCTGTGG GGATGATGAC GCCAAGTACA CCTGCAAGGC TGTCAACAGC
    CTCGGAGAAG CCACCTGCAC CGCGGAGCTC ATCGTGGAGA CCATGGAGGA AGGGGAAGGG
    GAGGAGGGGG GGGAGGAGGA GGAGGAGGAG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.