Mylk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mylk
Entrez Gene ID	107589
Full Name	myosin, light polypeptide kinase
Synonyms	9530072E15Rik,A930019C19Rik,AW489456,KRP,MLCK108,MLCK210,Mlck,nmMlck,smMLCK
General protein information	Preferred Names myosin, light polypeptide kinase Names myosin light chain kinase, smooth muscle kinase-related protein mylk telokin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006521703.3	XP_006521766.1	myosin light chain kinase, smooth muscle isoform X1
XM_006521705.3	XP_006521768.1	myosin light chain kinase, smooth muscle isoform X3
NM_139300.3	NP_647461.3	myosin light chain kinase, smooth muscle
XM_017316849.1	XP_017172338.1	myosin light chain kinase, smooth muscle isoform X2
XM_006521705.2	XP_006521768.1	myosin light chain kinase, smooth muscle isoform X3
XM_017316849.2	XP_017172338.1	myosin light chain kinase, smooth muscle isoform X2
XM_006521702.1	XP_006521765.1	myosin light chain kinase, smooth muscle isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYLK	XP_005639631.1
	MYLK	XP_001113525.1
Gallus gallus (chicken)	MYLK	NP_990790.1
Xenopus tropicalis (tropical clawed frog)	LOC100489678	XP_002935598.2
Homo sapiens (human)	MYLK	NP_444253.3
Pan troglodytes (chimpanzee)	MYLK	XP_003950224.1
Mus musculus (house mouse)	Mylk	NP_647461.3
Rattus norvegicus (Norway rat)	Mylk	NP_001099344.2
Danio rerio (zebrafish)	mylkb	XP_002662355.2

Gene Ontology

The following Mylk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mylk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu51813	XM_006521705.3 Latest version!	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu79248	XM_017316849.2 Latest version!	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20865	NM_139300.3 Latest version!	Mus musculus myosin, light polypeptide kinase (Mylk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1434.30 $2049.00
OMu51811	XM_006521703.3 Latest version!	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu51811	XM_006521702.1 Latest version!	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu51813	XM_006521705.2	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu79248	XM_017316849.1	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mylk ( XM_006521705.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521768.1 Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu51813
Clone ID Related Accession (Same CDS sequence)	XM_006521705.3 , XM_006521705.2
Accession Version	XM_006521705.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase, smooth muscle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521705.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC GGAGACCCAA GAGCTCTCTT CCCCCTGTGC TAGGAACAGA GAGCGACGCA 
ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCCACAAAAG CAGCTATGCC CCCTCAAATC 
ATCCAGTTCC CCGAGGACCA GAAGGTGCGC GCAGGAGAAC CTGTGGAGCT GTTTGGGAAA 
GTGGCTGGCA CCCAGCCCAT CACCTGCAAG TGGATGAAGT TCCGAAAGCA GATCCAGGAG 
AGCGAGCATA TCAAGGTGGA GAATGGCGAG AGCGGCAGCA AGCTCACCAT CCTGGCCGCG 
CGGCAGGAGC ACTGTGGCTG CTACACACTG GTGGTGGAGA ACAAGCTGGG CAGCAGGCAG 
GCCCAAGTCA ACCTCACGGT CGTGGATAAG CCAGACCCCC CGGCTGGCAC ACCTTGTGCT 
TCTGACATTC GGAGTTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC 
AGTGCTGTAC AGTCCTACAA TGTGGAGATC TGGGACACAG AGGACAAGGT GTGGAAAGAG 
CTAGCCACGT GCCGCAGCAC CTCTTTTAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAC 
AAATTCCGAG TGCGTGCAGT CAACGTCTAT GGAACCAGCG AGCCAAGCCA AGAGTCCGAG 
CTCACAGCTG TGGGAGAGAA ACCTGAAGAG CCAAAGGATG AAGTGGAAGT GTCCGATGAT 
GACGAAAAGG AACCTGAGGT TGATTACCGG ACGGTGACTG TTAACACTGA ACAGAAAGTG 
TCTGATGTGT ATGACATTGA AGAGCGACTA GGATCTGGGA AGTTTGGACA AGTCTTCCGA 
CTTGTGGAAA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TTTTCAAGGC CTATTCCGCC 
AAGGAGAAAG ACAACATCCG TCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG 
CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TTGTCATGGT TCTGGAGATC 
GTGTCCGGGG GTGAGCTGTT TGAGCGTATC ATTGACGAGG ACTTCGAGCT GACGGAGCGG 
GAGTGTATCA AGTACATGAG GCAGATCTCG GAAGGGGTGG AGTACATCCA CAAGCAGGGC 
ATTGTGCACC TGGACCTCAA GCCCGAGAAC ATCATGTGTG TCAACAAGAC AGGCACCAGG 
ATCAAGCTCA TTGACTTCGG CCTGGCACGG AGACTCGAGA ACGCTGGGTC TCTGAAGGTC 
CTCTTTGGGA CCCCAGAGTT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC 
GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTATATTC TGGTCAGCGG ACTTTCTCCC 
TTCATGGGTG ACAATGATAA CGAGACCTTA GCCAACGTCA CTTCAGCCAC CTGGGACTTC 
GATGACGAGG CATTCGATGA GATCTCCGAT GACGCAAAGG ATTTCATCAG TAACTTGCTG 
AAGAAAGATA TGAAAAACCG TCTGGACTGC ACCCAGTGCC TTCAGCACCC ATGGCTGATG 
AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGGATGAA GAAATACATG 
GCAAGAAGGA AGTGGCAGAA AACGGGCAAT GCTGTGAGAG CCATCGGGAG ACTGTCCTCT 
ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCTTCAA CAGGGTCACC AACCAGCCCA 
ATCAATGCGG AGAAACTAGA GTCTGAAGAC GATGTCTCCC AAGCTTTCCT GGAGGCTGTT 
GCTGAGGAAA AGCCCCATGT GAAACCTTAT TTCTCTAAGA CCATCCGTGA TCTGGAAGTC 
GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTG 
GTCTGGTTCA AGGATGACCA ATCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAC 
GAAGACGGCA ACTGCTCGCT CATCATCAGC GACGTCTGTG GGGATGATGA CGCCAAGTAC 
ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CTGCGGAGCT CATCGTGGAG 
ACCATGGAGG AAGGGGAAGG GGAGGAGGGG GGAGAGGAGG AGGAGGAGGA GGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521768.1
CDS	126..2285
Translation	MKSRRPKSSLPPVLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE

Target ORF information:

RefSeq Version	XM_006521705.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521705.2

ORF Insert Sequence:

ATGAAATCCC GGAGACCCAA GAGCTCTCTT CCCCCTGTGC TAGGAACAGA GAGCGACGCA 
ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCCACAAAAG CAGCTATGCC CCCTCAAATC 
ATCCAGTTCC CCGAGGACCA GAAGGTGCGC GCAGGAGAAC CTGTGGAGCT GTTTGGGAAA 
GTGGCTGGCA CCCAGCCCAT CACCTGCAAG TGGATGAAGT TCCGAAAGCA GATCCAGGAG 
AGCGAGCATA TCAAGGTGGA GAATGGCGAG AGCGGCAGCA AGCTCACCAT CCTGGCCGCG 
CGGCAGGAGC ACTGTGGCTG CTACACACTG GTGGTGGAGA ACAAGCTGGG CAGCAGGCAG 
GCCCAAGTCA ACCTCACGGT CGTGGATAAG CCAGACCCCC CGGCTGGCAC ACCTTGTGCT 
TCTGACATTC GGAGTTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC 
AGTGCTGTAC AGTCCTACAA TGTGGAGATC TGGGACACAG AGGACAAGGT GTGGAAAGAG 
CTAGCCACGT GCCGCAGCAC CTCTTTTAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAC 
AAATTCCGAG TGCGTGCAGT CAACGTCTAT GGAACCAGCG AGCCAAGCCA AGAGTCCGAG 
CTCACAGCTG TGGGAGAGAA ACCTGAAGAG CCAAAGGATG AAGTGGAAGT GTCCGATGAT 
GACGAAAAGG AACCTGAGGT TGATTACCGG ACGGTGACTG TTAACACTGA ACAGAAAGTG 
TCTGATGTGT ATGACATTGA AGAGCGACTA GGATCTGGGA AGTTTGGACA AGTCTTCCGA 
CTTGTGGAAA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TTTTCAAGGC CTATTCCGCC 
AAGGAGAAAG ACAACATCCG TCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG 
CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TTGTCATGGT TCTGGAGATC 
GTGTCCGGGG GTGAGCTGTT TGAGCGTATC ATTGACGAGG ACTTCGAGCT GACGGAGCGG 
GAGTGTATCA AGTACATGAG GCAGATCTCG GAAGGGGTGG AGTACATCCA CAAGCAGGGC 
ATTGTGCACC TGGACCTCAA GCCCGAGAAC ATCATGTGTG TCAACAAGAC AGGCACCAGG 
ATCAAGCTCA TTGACTTCGG CCTGGCACGG AGACTCGAGA ACGCTGGGTC TCTGAAGGTC 
CTCTTTGGGA CCCCAGAGTT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC 
GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTATATTC TGGTCAGCGG ACTTTCTCCC 
TTCATGGGTG ACAATGATAA CGAGACCTTA GCCAACGTCA CTTCAGCCAC CTGGGACTTC 
GATGACGAGG CATTCGATGA GATCTCCGAT GACGCAAAGG ATTTCATCAG TAACTTGCTG 
AAGAAAGATA TGAAAAACCG TCTGGACTGC ACCCAGTGCC TTCAGCACCC ATGGCTGATG 
AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGGATGAA GAAATACATG 
GCAAGAAGGA AGTGGCAGAA AACGGGCAAT GCTGTGAGAG CCATCGGGAG ACTGTCCTCT 
ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCTTCAA CAGGGTCACC AACCAGCCCA 
ATCAATGCGG AGAAACTAGA GTCTGAAGAC GATGTCTCCC AAGCTTTCCT GGAGGCTGTT 
GCTGAGGAAA AGCCCCATGT GAAACCTTAT TTCTCTAAGA CCATCCGTGA TCTGGAAGTC 
GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTG 
GTCTGGTTCA AGGATGACCA ATCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAC 
GAAGACGGCA ACTGCTCGCT CATCATCAGC GACGTCTGTG GGGATGATGA CGCCAAGTAC 
ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CTGCGGAGCT CATCGTGGAG 
ACCATGGAGG AAGGGGAAGG GGAGGAGGGG GGAGAGGAGG AGGAGGAGGA GGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_006521705.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521768.1 Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu51813
Clone ID Related Accession (Same CDS sequence)	XM_006521705.3 , XM_006521705.2
Accession Version	XM_006521705.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase, smooth muscle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCC GGAGACCCAA GAGCTCTCTT CCCCCTGTGC TAGGAACAGA GAGCGACGCA 
ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCCACAAAAG CAGCTATGCC CCCTCAAATC 
ATCCAGTTCC CCGAGGACCA GAAGGTGCGC GCAGGAGAAC CTGTGGAGCT GTTTGGGAAA 
GTGGCTGGCA CCCAGCCCAT CACCTGCAAG TGGATGAAGT TCCGAAAGCA GATCCAGGAG 
AGCGAGCATA TCAAGGTGGA GAATGGCGAG AGCGGCAGCA AGCTCACCAT CCTGGCCGCG 
CGGCAGGAGC ACTGTGGCTG CTACACACTG GTGGTGGAGA ACAAGCTGGG CAGCAGGCAG 
GCCCAAGTCA ACCTCACGGT CGTGGATAAG CCAGACCCCC CGGCTGGCAC ACCTTGTGCT 
TCTGACATTC GGAGTTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC 
AGTGCTGTAC AGTCCTACAA TGTGGAGATC TGGGACACAG AGGACAAGGT GTGGAAAGAG 
CTAGCCACGT GCCGCAGCAC CTCTTTTAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAC 
AAATTCCGAG TGCGTGCAGT CAACGTCTAT GGAACCAGCG AGCCAAGCCA AGAGTCCGAG 
CTCACAGCTG TGGGAGAGAA ACCTGAAGAG CCAAAGGATG AAGTGGAAGT GTCCGATGAT 
GACGAAAAGG AACCTGAGGT TGATTACCGG ACGGTGACTG TTAACACTGA ACAGAAAGTG 
TCTGATGTGT ATGACATTGA AGAGCGACTA GGATCTGGGA AGTTTGGACA AGTCTTCCGA 
CTTGTGGAAA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TTTTCAAGGC CTATTCCGCC 
AAGGAGAAAG ACAACATCCG TCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG 
CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TTGTCATGGT TCTGGAGATC 
GTGTCCGGGG GTGAGCTGTT TGAGCGTATC ATTGACGAGG ACTTCGAGCT GACGGAGCGG 
GAGTGTATCA AGTACATGAG GCAGATCTCG GAAGGGGTGG AGTACATCCA CAAGCAGGGC 
ATTGTGCACC TGGACCTCAA GCCCGAGAAC ATCATGTGTG TCAACAAGAC AGGCACCAGG 
ATCAAGCTCA TTGACTTCGG CCTGGCACGG AGACTCGAGA ACGCTGGGTC TCTGAAGGTC 
CTCTTTGGGA CCCCAGAGTT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC 
GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTATATTC TGGTCAGCGG ACTTTCTCCC 
TTCATGGGTG ACAATGATAA CGAGACCTTA GCCAACGTCA CTTCAGCCAC CTGGGACTTC 
GATGACGAGG CATTCGATGA GATCTCCGAT GACGCAAAGG ATTTCATCAG TAACTTGCTG 
AAGAAAGATA TGAAAAACCG TCTGGACTGC ACCCAGTGCC TTCAGCACCC ATGGCTGATG 
AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGGATGAA GAAATACATG 
GCAAGAAGGA AGTGGCAGAA AACGGGCAAT GCTGTGAGAG CCATCGGGAG ACTGTCCTCT 
ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCTTCAA CAGGGTCACC AACCAGCCCA 
ATCAATGCGG AGAAACTAGA GTCTGAAGAC GATGTCTCCC AAGCTTTCCT GGAGGCTGTT 
GCTGAGGAAA AGCCCCATGT GAAACCTTAT TTCTCTAAGA CCATCCGTGA TCTGGAAGTC 
GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTG 
GTCTGGTTCA AGGATGACCA ATCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAC 
GAAGACGGCA ACTGCTCGCT CATCATCAGC GACGTCTGTG GGGATGATGA CGCCAAGTAC 
ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CTGCGGAGCT CATCGTGGAG 
ACCATGGAGG AAGGGGAAGG GGAGGAGGGG GGAGAGGAGG AGGAGGAGGA GGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521768.1
CDS	126..2285
Translation	MKSRRPKSSLPPVLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE

Target ORF information:

RefSeq Version	XM_006521705.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521705.3

ORF Insert Sequence:

ATGAAATCCC GGAGACCCAA GAGCTCTCTT CCCCCTGTGC TAGGAACAGA GAGCGACGCA 
ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCCACAAAAG CAGCTATGCC CCCTCAAATC 
ATCCAGTTCC CCGAGGACCA GAAGGTGCGC GCAGGAGAAC CTGTGGAGCT GTTTGGGAAA 
GTGGCTGGCA CCCAGCCCAT CACCTGCAAG TGGATGAAGT TCCGAAAGCA GATCCAGGAG 
AGCGAGCATA TCAAGGTGGA GAATGGCGAG AGCGGCAGCA AGCTCACCAT CCTGGCCGCG 
CGGCAGGAGC ACTGTGGCTG CTACACACTG GTGGTGGAGA ACAAGCTGGG CAGCAGGCAG 
GCCCAAGTCA ACCTCACGGT CGTGGATAAG CCAGACCCCC CGGCTGGCAC ACCTTGTGCT 
TCTGACATTC GGAGTTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC 
AGTGCTGTAC AGTCCTACAA TGTGGAGATC TGGGACACAG AGGACAAGGT GTGGAAAGAG 
CTAGCCACGT GCCGCAGCAC CTCTTTTAAT GTCCAAGACC TGCTGCCTGA CCGCGAGTAC 
AAATTCCGAG TGCGTGCAGT CAACGTCTAT GGAACCAGCG AGCCAAGCCA AGAGTCCGAG 
CTCACAGCTG TGGGAGAGAA ACCTGAAGAG CCAAAGGATG AAGTGGAAGT GTCCGATGAT 
GACGAAAAGG AACCTGAGGT TGATTACCGG ACGGTGACTG TTAACACTGA ACAGAAAGTG 
TCTGATGTGT ATGACATTGA AGAGCGACTA GGATCTGGGA AGTTTGGACA AGTCTTCCGA 
CTTGTGGAAA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TTTTCAAGGC CTATTCCGCC 
AAGGAGAAAG ACAACATCCG TCAGGAGATC AGCATCATGA ACTGCCTCCA CCACCCCAAG 
CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TTGTCATGGT TCTGGAGATC 
GTGTCCGGGG GTGAGCTGTT TGAGCGTATC ATTGACGAGG ACTTCGAGCT GACGGAGCGG 
GAGTGTATCA AGTACATGAG GCAGATCTCG GAAGGGGTGG AGTACATCCA CAAGCAGGGC 
ATTGTGCACC TGGACCTCAA GCCCGAGAAC ATCATGTGTG TCAACAAGAC AGGCACCAGG 
ATCAAGCTCA TTGACTTCGG CCTGGCACGG AGACTCGAGA ACGCTGGGTC TCTGAAGGTC 
CTCTTTGGGA CCCCAGAGTT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC 
GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTATATTC TGGTCAGCGG ACTTTCTCCC 
TTCATGGGTG ACAATGATAA CGAGACCTTA GCCAACGTCA CTTCAGCCAC CTGGGACTTC 
GATGACGAGG CATTCGATGA GATCTCCGAT GACGCAAAGG ATTTCATCAG TAACTTGCTG 
AAGAAAGATA TGAAAAACCG TCTGGACTGC ACCCAGTGCC TTCAGCACCC ATGGCTGATG 
AAAGACACCA AGAACATGGA GGCCAAGAAG CTGTCCAAGG ACCGGATGAA GAAATACATG 
GCAAGAAGGA AGTGGCAGAA AACGGGCAAT GCTGTGAGAG CCATCGGGAG ACTGTCCTCT 
ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCTTCAA CAGGGTCACC AACCAGCCCA 
ATCAATGCGG AGAAACTAGA GTCTGAAGAC GATGTCTCCC AAGCTTTCCT GGAGGCTGTT 
GCTGAGGAAA AGCCCCATGT GAAACCTTAT TTCTCTAAGA CCATCCGTGA TCTGGAAGTC 
GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAGGTG 
GTCTGGTTCA AGGATGACCA ATCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAC 
GAAGACGGCA ACTGCTCGCT CATCATCAGC GACGTCTGTG GGGATGATGA CGCCAAGTAC 
ACCTGCAAGG CTGTCAACAG CCTCGGAGAA GCCACCTGCA CTGCGGAGCT CATCGTGGAG 
ACCATGGAGG AAGGGGAAGG GGAGGAGGGG GGAGAGGAGG AGGAGGAGGA GGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_017316849.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172338.1 Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu79248
Clone ID Related Accession (Same CDS sequence)	XM_017316849.1 , XM_017316849.2
Accession Version	XM_017316849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase, smooth muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGTT TGCCTCGTCA CACATGTCTA AAACCTCCCA CAGTGTGGAT 
CCCTCGAAGG TCAGCTCCAT GCCTCTCACC GAGGCCCCAG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACAGCC AAGTTCGAAG GGAGGGTCCG GGGCTACCCA 
GAGCCCCAGG TGACATGGCA CAGAAAAGGG CAAGCCATCA CCAATGGTGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTTAGT CTTGTCATCC ACACTGTCCG CGAGGAGGAC 
AAGGGCAAAT ACACCTGTGA AGCTAGCAAT GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGAATTC TATGAAGAAG CGTGATCAGC CTGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCACCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTCTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGTGTGTCAA TGTCAGAGAA GAATGGCATG CAGATCCTGG AGATCCGTGG GGTCACTCGG 
GATGACCTGG GCGTGTACAC CTGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCG 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCGCTAGGT TGGCTGTGAG AGGAACCAAA 
GCCCCCAGTC CAGACATCAG GAAGGAGGTG ACCAATGGAG TCTCCAAAGA CCCAGAGACT 
GTGGCTGAAA GTAAGAACTG TCCAAGTCCC CAGAGGAGTG GCTCCTCAGC CCGGGCCACC 
AACAGTCACC TCAAGTCCCC GCAGGAGCCC AAGCCAAAGC TGTGTGAGGA TGCTCCCAGA 
AAGGTCCCGC AGTCCTCCAT CCTGCAGAAG TCCACCAGCA CCATCACTCT GCAGGCCTTG 
AAAGTCCAGC CAGAAGCGAG AGTACCAGCC ATAGGGTCCT TCTCTCCTGG GGAAGACAGG 
AAGAGTCTGG CTGCCCCTCA GCAAGCCACA CTCCCTACCA GACAGTCTAG CCTGGGAGGT 
TCCGTTGGGA ACAAGTTTGT TACCGGGAAC ATCCCCAGAG AGAGCCAAAG GGAGTCAACA 
TTCCCCAGAT TTGAGAGTCA GCCCCAAAGC CAGGAAGTCA CTGAAGGTCA GACAGTCAAG 
TTCATCTGCG AGGTTTCAGG TATACCAAAG CCTGACGTGG GCTGGTTCCT GGAAGGCATC 
CCCGTGAGGA GACGGGAAGG CATCACTGAG GTTTATGAAG ATGGTGTATC CCATCACCTG 
TGCCTGCTGA GAGCCCGGAC GAGAGACAGT GGGAGGTACA GCTGCACAGC ATCCAATAGC 
CTTGGCCAGG TGTCCTGCAG CTGGAGCCTG CTGGTGGACC GGCCGAACTT GGCACAGACA 
GCCCCTTCAT TCTCTAGTGT TCTGAAGGAC AGTGTTGTCA TTGAGGGCCA GGATTTTGTG 
CTACGGTGCT CAGTGCAGGG GACTCCAGCA CCCCGGGTCA CGTGGCTGCT CAATGGACAG 
CCCATCCAGT TTGCTCACTC CATCTGTGAG GCAGGCGTGG CTGAGCTCCA CATCCAGGAT 
GCCCTGCCAG AGGACCGTGG CACCTACACC TGCCTGGCTG AGAATGCCAT GGGGCAGGTG 
TCCTGCAGCG CCACTGTCAC CGTCCAAGGG AACCCACCTC CGGAAGTCAT CTGGCTTCAC 
GACGGGAATG AGATTCAGGA GTCCGAGGAC TTCCACTTCG AGCAGAAGGG TGGCTGGCAC 
AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG GACACGGGCA CGTACACCTG TGAGGCCTGG 
AACAGTGCCG GGGAGGTCCG CACCCGGGCC GTCCTCACCG TGCAAGAGCC TCATGATGGC 
ACCCAGCCCT GGTTCATCAG CAAGCCTCGT TCGGTGACAG CCACCCTGGG CCAGAGTGTC 
CTCATCTCCT GCGCCATAGC TGGAGACCCC TTTCCCACCG TGCACTGGCT CCGGGATGGC 
AGAGCCCTCT CCAAGGACAG TGGCCACTTT GAGCTGCTCC AGAATGAGGA CGTGTTCACC 
CTGGTTCTAA AGAACGTGCA GCCCTGGCAT GCCGGCCAGT ATGAGATTCT GCTCAAGAAC 
CGGGTTGGCG AGTGTAGTTG CCAAGTGTCA CTGATGCTTC ACAACAGCCC TTCCAGAGCC 
CCACCACGGG GGCGGGAGCC TGCCAGCTGC GAGGGCCTCT GTGGTGGTGG AGGAGTTGGT 
GCTCATGGTG ATGGTGACCG TCATGGGACC CTGAGGCCCT GCTGGCCAGC AAGAGGGCAA 
GGTTGGCCTG AGGAGGAAGA CGGAGAAGAC GTGAGGGGGC TGTTGAAGAG ACGGGTGGAG 
ACCAGGCTGC ACACAGAAGA GGCCATCCGC CAGCAGGAGG TGGGGCAGCT GGATTTTCGA 
GACCTCCTGG GAAAGAAGGT GAGCACCAAG ACCGTCTCAG AAGACGACCT GAAGGACATC 
CCAGCCGAGC AGATGGATTT CCGCGCCAAC CTTCAGCGGC AAGTGAAGCC AAAGACCATA 
TCCGAGGAAG AGAGGAAGGT GCATAGCCCT CAGCAGGTTG ACTTCCGGTC TGTCCTCGCC 
AAGAAGGGGA CCCCTAAGAC CCCCGTTCCT GAGAAGGCAC CTCCAAAAGC TGCCACCCCA 
GACTTTCGCT CTGTGCTAGG TGGCAAGAAG AAGTCACCCT CGGAGAATGG GGGCAACAGC 
GCTGAGGTCT TGAATGTCAA GGCTGGGGAA AGCCCCACAC CTGCAGGCGA TGCGCAGGCC 
ATCGGGGCCC TGAAACCCGT AGGCAATGCC AAGCCTGCTG AGACCCCGAA ACCTATAGGT 
AATGCCAAGC CTACCGAGAC CCTGAAGCCT GTGGGCAACA CCAAGCCTGC CGAGACCCTG 
AAACCCATAG CCAACGCACA GCCTTCAGGG TCCCTGAAAC CTGTAACCAA CGCACAACCT 
GCTGAGCCCC AGAAGCCTGT GGGCAATGCC AAGTCTGCTG AGACCTCGAA ACCAGCTGGG 
AAAGAAGAAG TCAAGGAGGT AAAGAATGAT GTGAACTGTA AGAAAGGGCA GGTCGGAGCC 
ACGGGCAATG AAAAGCGACC CGAGAGCCAA GGCTCGGCCC CAGTTTTCAA GGAAAAGCTA 
CAAGATGTCC ATGTGGCAGA GGGGGAGAAG CTGCTACTCC AATGCCAGGT GATCTCTGAT 
CCCCCCGCCA CTGTCACCTG GTCGCTGAAT GGGAAGACAC TCAAGACCAC AAAATTCATC 
GTCCTCGCCC AAGAAGGCTC ACGTTTCTCT GTCTCCATCG AGAAGGCACT GCCTGAGGAC 
CGAGGTTTGT ACAAGTGTGT AGCCAAGAAC AGCGCTGGCC AGGCAGAGTG TTCCTGCCAA 
GTCACTGTGG ACGATGCTCA GACCAGTGAG AACACCAAGG CCCCGGAGAT GAAATCCCGG 
AGACCCAAGA GCTCTCTTCC CCCTGTGCTA GGAACAGAGA GCGACGCAAC TGTGAAAAAG 
AAACCTGCCC CCAAGACACC CACAAAAGCA GCTATGCCCC CTCAAATCAT CCAGTTCCCC 
GAGGACCAGA AGGTGCGCGC AGGAGAACCT GTGGAGCTGT TTGGGAAAGT GGCTGGCACC 
CAGCCCATCA CCTGCAAGTG GATGAAGTTC CGAAAGCAGA TCCAGGAGAG CGAGCATATC 
AAGGTGGAGA ATGGCGAGAG CGGCAGCAAG CTCACCATCC TGGCCGCGCG GCAGGAGCAC 
TGTGGCTGCT ACACACTGGT GGTGGAGAAC AAGCTGGGCA GCAGGCAGGC CCAAGTCAAC 
CTCACGGTCG TGGATAAGCC AGACCCCCCG GCTGGCACAC CTTGTGCTTC TGACATTCGG 
AGTTCCTCAC TGACCCTGTC CTGGTATGGC TCCTCATATG ATGGGGGCAG TGCTGTACAG 
TCCTACAATG TGGAGATCTG GGACACAGAG GACAAGGTGT GGAAAGAGCT AGCCACGTGC 
CGCAGCACCT CTTTTAATGT CCAAGACCTG CTGCCTGACC GCGAGTACAA ATTCCGAGTG 
CGTGCAGTCA ACGTCTATGG AACCAGCGAG CCAAGCCAAG AGTCCGAGCT CACAGCTGTG 
GGAGAGAAAC CTGAAGAGCC AAAGGATGAA GTGGAAGTGT CCGATGATGA CGAAAAGGAA 
CCTGAGGTTG ATTACCGGAC GGTGACTGTT AACACTGAAC AGAAAGTGTC TGATGTGTAT 
GACATTGAAG AGCGACTAGG ATCTGGGAAG TTTGGACAAG TCTTCCGACT TGTGGAAAAG 
AAAACTGGAA AAATCTGGGC AGGGAAATTT TTCAAGGCCT ATTCCGCCAA GGAGAAAGAC 
AACATCCGTC AGGAGATCAG CATCATGAAC TGCCTCCACC ACCCCAAGCT GGTCCAGTGT 
GTGGATGCCT TCGAAGAAAA GGCCAACATT GTCATGGTTC TGGAGATCGT GTCCGGGGGT 
GAGCTGTTTG AGCGTATCAT TGACGAGGAC TTCGAGCTGA CGGAGCGGGA GTGTATCAAG 
TACATGAGGC AGATCTCGGA AGGGGTGGAG TACATCCACA AGCAGGGCAT TGTGCACCTG 
GACCTCAAGC CCGAGAACAT CATGTGTGTC AACAAGACAG GCACCAGGAT CAAGCTCATT 
GACTTCGGCC TGGCACGGAG ACTCGAGAAC GCTGGGTCTC TGAAGGTCCT CTTTGGGACC 
CCAGAGTTTG TGGCTCCTGA AGTGATCAAC TATGAGCCCA TCGGCTACGC CACAGACATG 
TGGAGCATCG GGGTCATCTG CTATATTCTG GTCAGCGGAC TTTCTCCCTT CATGGGTGAC 
AATGATAACG AGACCTTAGC CAACGTCACT TCAGCCACCT GGGACTTCGA TGACGAGGCA 
TTCGATGAGA TCTCCGATGA CGCAAAGGAT TTCATCAGTA ACTTGCTGAA GAAAGATATG 
AAAAACCGTC TGGACTGCAC CCAGTGCCTT CAGCACCCAT GGCTGATGAA AGACACCAAG 
AACATGGAGG CCAAGAAGCT GTCCAAGGAC CGGATGAAGA AATACATGGC AAGAAGGAAG 
TGGCAGAAAA CGGGCAATGC TGTGAGAGCC ATCGGGAGAC TGTCCTCTAT GGCAATGATC 
TCAGGGCTCA GTGGCAGGAA ATCTTCAACA GGGTCACCAA CCAGCCCAAT CAATGCGGAG 
AAACTAGAGT CTGAAGACGA TGTCTCCCAA GCTTTCCTGG AGGCTGTTGC TGAGGAAAAG 
CCCCATGTGA AACCTTATTT CTCTAAGACC ATCCGTGATC TGGAAGTCGT GGAGGGAAGC 
GCTGCTAGAT TTGACTGCAA GATTGAAGGA TACCCAGACC CTGAGGTGGT CTGGTTCAAG 
GATGACCAAT CAATCAGGGA GTCCCGCCAC TTCCAGATAG ACTACGACGA AGACGGCAAC 
TGCTCGCTCA TCATCAGCGA CGTCTGTGGG GATGATGACG CCAAGTACAC CTGCAAGGCT 
GTCAACAGCC TCGGAGAAGC CACCTGCACT GCGGAGCTCA TCGTGGAGAC CATGGAGGAA 
GGGGAAGGGG AGGAGGGGGG AGAGGAGGAG GAGGAGGAGG AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172338.1
CDS	176..5863
Translation	MGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRNLCVKEGATAKFEGRVRGYPEPQVTWHRKGQAITNGGRFLLDCGVRGTFSLVIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKRDQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSAELSIPGLDNAARLAVRGTKAPSPDIRKEVTNGVSKDPETVAESKNCPSPQRSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALKVQPEARVPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSVGNKFVTGNIPRESQRESTFPRFESQPQSQEVTEGQTVKFICEVSGIPKPDVGWFLEGIPVRRREGITEVYEDGVSHHLCLLRARTRDSGRYSCTASNSLGQVSCSWSLLVDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQPIQFAHSICEAGVAELHIQDALPEDRGTYTCLAENAMGQVSCSATVTVQGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVHWLRDGRALSKDSGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDGDRHGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKAPPKAATPDFRSVLGGKKKSPSENGGNSAEVLNVKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTKPAETLKPIANAQPSGSLKPVTNAQPAEPQKPVGNAKSAETSKPAGKEEVKEVKNDVNCKKGQVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVISDPPATVTWSLNGKTLKTTKFIVLAQEGSRFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE

Target ORF information:

RefSeq Version	XM_017316849.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316849.2

ORF Insert Sequence:

ATGGGGGACG TGAAACTGTT TGCCTCGTCA CACATGTCTA AAACCTCCCA CAGTGTGGAT 
CCCTCGAAGG TCAGCTCCAT GCCTCTCACC GAGGCCCCAG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACAGCC AAGTTCGAAG GGAGGGTCCG GGGCTACCCA 
GAGCCCCAGG TGACATGGCA CAGAAAAGGG CAAGCCATCA CCAATGGTGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTTAGT CTTGTCATCC ACACTGTCCG CGAGGAGGAC 
AAGGGCAAAT ACACCTGTGA AGCTAGCAAT GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGAATTC TATGAAGAAG CGTGATCAGC CTGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCACCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTCTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGTGTGTCAA TGTCAGAGAA GAATGGCATG CAGATCCTGG AGATCCGTGG GGTCACTCGG 
GATGACCTGG GCGTGTACAC CTGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCG 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCGCTAGGT TGGCTGTGAG AGGAACCAAA 
GCCCCCAGTC CAGACATCAG GAAGGAGGTG ACCAATGGAG TCTCCAAAGA CCCAGAGACT 
GTGGCTGAAA GTAAGAACTG TCCAAGTCCC CAGAGGAGTG GCTCCTCAGC CCGGGCCACC 
AACAGTCACC TCAAGTCCCC GCAGGAGCCC AAGCCAAAGC TGTGTGAGGA TGCTCCCAGA 
AAGGTCCCGC AGTCCTCCAT CCTGCAGAAG TCCACCAGCA CCATCACTCT GCAGGCCTTG 
AAAGTCCAGC CAGAAGCGAG AGTACCAGCC ATAGGGTCCT TCTCTCCTGG GGAAGACAGG 
AAGAGTCTGG CTGCCCCTCA GCAAGCCACA CTCCCTACCA GACAGTCTAG CCTGGGAGGT 
TCCGTTGGGA ACAAGTTTGT TACCGGGAAC ATCCCCAGAG AGAGCCAAAG GGAGTCAACA 
TTCCCCAGAT TTGAGAGTCA GCCCCAAAGC CAGGAAGTCA CTGAAGGTCA GACAGTCAAG 
TTCATCTGCG AGGTTTCAGG TATACCAAAG CCTGACGTGG GCTGGTTCCT GGAAGGCATC 
CCCGTGAGGA GACGGGAAGG CATCACTGAG GTTTATGAAG ATGGTGTATC CCATCACCTG 
TGCCTGCTGA GAGCCCGGAC GAGAGACAGT GGGAGGTACA GCTGCACAGC ATCCAATAGC 
CTTGGCCAGG TGTCCTGCAG CTGGAGCCTG CTGGTGGACC GGCCGAACTT GGCACAGACA 
GCCCCTTCAT TCTCTAGTGT TCTGAAGGAC AGTGTTGTCA TTGAGGGCCA GGATTTTGTG 
CTACGGTGCT CAGTGCAGGG GACTCCAGCA CCCCGGGTCA CGTGGCTGCT CAATGGACAG 
CCCATCCAGT TTGCTCACTC CATCTGTGAG GCAGGCGTGG CTGAGCTCCA CATCCAGGAT 
GCCCTGCCAG AGGACCGTGG CACCTACACC TGCCTGGCTG AGAATGCCAT GGGGCAGGTG 
TCCTGCAGCG CCACTGTCAC CGTCCAAGGG AACCCACCTC CGGAAGTCAT CTGGCTTCAC 
GACGGGAATG AGATTCAGGA GTCCGAGGAC TTCCACTTCG AGCAGAAGGG TGGCTGGCAC 
AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG GACACGGGCA CGTACACCTG TGAGGCCTGG 
AACAGTGCCG GGGAGGTCCG CACCCGGGCC GTCCTCACCG TGCAAGAGCC TCATGATGGC 
ACCCAGCCCT GGTTCATCAG CAAGCCTCGT TCGGTGACAG CCACCCTGGG CCAGAGTGTC 
CTCATCTCCT GCGCCATAGC TGGAGACCCC TTTCCCACCG TGCACTGGCT CCGGGATGGC 
AGAGCCCTCT CCAAGGACAG TGGCCACTTT GAGCTGCTCC AGAATGAGGA CGTGTTCACC 
CTGGTTCTAA AGAACGTGCA GCCCTGGCAT GCCGGCCAGT ATGAGATTCT GCTCAAGAAC 
CGGGTTGGCG AGTGTAGTTG CCAAGTGTCA CTGATGCTTC ACAACAGCCC TTCCAGAGCC 
CCACCACGGG GGCGGGAGCC TGCCAGCTGC GAGGGCCTCT GTGGTGGTGG AGGAGTTGGT 
GCTCATGGTG ATGGTGACCG TCATGGGACC CTGAGGCCCT GCTGGCCAGC AAGAGGGCAA 
GGTTGGCCTG AGGAGGAAGA CGGAGAAGAC GTGAGGGGGC TGTTGAAGAG ACGGGTGGAG 
ACCAGGCTGC ACACAGAAGA GGCCATCCGC CAGCAGGAGG TGGGGCAGCT GGATTTTCGA 
GACCTCCTGG GAAAGAAGGT GAGCACCAAG ACCGTCTCAG AAGACGACCT GAAGGACATC 
CCAGCCGAGC AGATGGATTT CCGCGCCAAC CTTCAGCGGC AAGTGAAGCC AAAGACCATA 
TCCGAGGAAG AGAGGAAGGT GCATAGCCCT CAGCAGGTTG ACTTCCGGTC TGTCCTCGCC 
AAGAAGGGGA CCCCTAAGAC CCCCGTTCCT GAGAAGGCAC CTCCAAAAGC TGCCACCCCA 
GACTTTCGCT CTGTGCTAGG TGGCAAGAAG AAGTCACCCT CGGAGAATGG GGGCAACAGC 
GCTGAGGTCT TGAATGTCAA GGCTGGGGAA AGCCCCACAC CTGCAGGCGA TGCGCAGGCC 
ATCGGGGCCC TGAAACCCGT AGGCAATGCC AAGCCTGCTG AGACCCCGAA ACCTATAGGT 
AATGCCAAGC CTACCGAGAC CCTGAAGCCT GTGGGCAACA CCAAGCCTGC CGAGACCCTG 
AAACCCATAG CCAACGCACA GCCTTCAGGG TCCCTGAAAC CTGTAACCAA CGCACAACCT 
GCTGAGCCCC AGAAGCCTGT GGGCAATGCC AAGTCTGCTG AGACCTCGAA ACCAGCTGGG 
AAAGAAGAAG TCAAGGAGGT AAAGAATGAT GTGAACTGTA AGAAAGGGCA GGTCGGAGCC 
ACGGGCAATG AAAAGCGACC CGAGAGCCAA GGCTCGGCCC CAGTTTTCAA GGAAAAGCTA 
CAAGATGTCC ATGTGGCAGA GGGGGAGAAG CTGCTACTCC AATGCCAGGT GATCTCTGAT 
CCCCCCGCCA CTGTCACCTG GTCGCTGAAT GGGAAGACAC TCAAGACCAC AAAATTCATC 
GTCCTCGCCC AAGAAGGCTC ACGTTTCTCT GTCTCCATCG AGAAGGCACT GCCTGAGGAC 
CGAGGTTTGT ACAAGTGTGT AGCCAAGAAC AGCGCTGGCC AGGCAGAGTG TTCCTGCCAA 
GTCACTGTGG ACGATGCTCA GACCAGTGAG AACACCAAGG CCCCGGAGAT GAAATCCCGG 
AGACCCAAGA GCTCTCTTCC CCCTGTGCTA GGAACAGAGA GCGACGCAAC TGTGAAAAAG 
AAACCTGCCC CCAAGACACC CACAAAAGCA GCTATGCCCC CTCAAATCAT CCAGTTCCCC 
GAGGACCAGA AGGTGCGCGC AGGAGAACCT GTGGAGCTGT TTGGGAAAGT GGCTGGCACC 
CAGCCCATCA CCTGCAAGTG GATGAAGTTC CGAAAGCAGA TCCAGGAGAG CGAGCATATC 
AAGGTGGAGA ATGGCGAGAG CGGCAGCAAG CTCACCATCC TGGCCGCGCG GCAGGAGCAC 
TGTGGCTGCT ACACACTGGT GGTGGAGAAC AAGCTGGGCA GCAGGCAGGC CCAAGTCAAC 
CTCACGGTCG TGGATAAGCC AGACCCCCCG GCTGGCACAC CTTGTGCTTC TGACATTCGG 
AGTTCCTCAC TGACCCTGTC CTGGTATGGC TCCTCATATG ATGGGGGCAG TGCTGTACAG 
TCCTACAATG TGGAGATCTG GGACACAGAG GACAAGGTGT GGAAAGAGCT AGCCACGTGC 
CGCAGCACCT CTTTTAATGT CCAAGACCTG CTGCCTGACC GCGAGTACAA ATTCCGAGTG 
CGTGCAGTCA ACGTCTATGG AACCAGCGAG CCAAGCCAAG AGTCCGAGCT CACAGCTGTG 
GGAGAGAAAC CTGAAGAGCC AAAGGATGAA GTGGAAGTGT CCGATGATGA CGAAAAGGAA 
CCTGAGGTTG ATTACCGGAC GGTGACTGTT AACACTGAAC AGAAAGTGTC TGATGTGTAT 
GACATTGAAG AGCGACTAGG ATCTGGGAAG TTTGGACAAG TCTTCCGACT TGTGGAAAAG 
AAAACTGGAA AAATCTGGGC AGGGAAATTT TTCAAGGCCT ATTCCGCCAA GGAGAAAGAC 
AACATCCGTC AGGAGATCAG CATCATGAAC TGCCTCCACC ACCCCAAGCT GGTCCAGTGT 
GTGGATGCCT TCGAAGAAAA GGCCAACATT GTCATGGTTC TGGAGATCGT GTCCGGGGGT 
GAGCTGTTTG AGCGTATCAT TGACGAGGAC TTCGAGCTGA CGGAGCGGGA GTGTATCAAG 
TACATGAGGC AGATCTCGGA AGGGGTGGAG TACATCCACA AGCAGGGCAT TGTGCACCTG 
GACCTCAAGC CCGAGAACAT CATGTGTGTC AACAAGACAG GCACCAGGAT CAAGCTCATT 
GACTTCGGCC TGGCACGGAG ACTCGAGAAC GCTGGGTCTC TGAAGGTCCT CTTTGGGACC 
CCAGAGTTTG TGGCTCCTGA AGTGATCAAC TATGAGCCCA TCGGCTACGC CACAGACATG 
TGGAGCATCG GGGTCATCTG CTATATTCTG GTCAGCGGAC TTTCTCCCTT CATGGGTGAC 
AATGATAACG AGACCTTAGC CAACGTCACT TCAGCCACCT GGGACTTCGA TGACGAGGCA 
TTCGATGAGA TCTCCGATGA CGCAAAGGAT TTCATCAGTA ACTTGCTGAA GAAAGATATG 
AAAAACCGTC TGGACTGCAC CCAGTGCCTT CAGCACCCAT GGCTGATGAA AGACACCAAG 
AACATGGAGG CCAAGAAGCT GTCCAAGGAC CGGATGAAGA AATACATGGC AAGAAGGAAG 
TGGCAGAAAA CGGGCAATGC TGTGAGAGCC ATCGGGAGAC TGTCCTCTAT GGCAATGATC 
TCAGGGCTCA GTGGCAGGAA ATCTTCAACA GGGTCACCAA CCAGCCCAAT CAATGCGGAG 
AAACTAGAGT CTGAAGACGA TGTCTCCCAA GCTTTCCTGG AGGCTGTTGC TGAGGAAAAG 
CCCCATGTGA AACCTTATTT CTCTAAGACC ATCCGTGATC TGGAAGTCGT GGAGGGAAGC 
GCTGCTAGAT TTGACTGCAA GATTGAAGGA TACCCAGACC CTGAGGTGGT CTGGTTCAAG 
GATGACCAAT CAATCAGGGA GTCCCGCCAC TTCCAGATAG ACTACGACGA AGACGGCAAC 
TGCTCGCTCA TCATCAGCGA CGTCTGTGGG GATGATGACG CCAAGTACAC CTGCAAGGCT 
GTCAACAGCC TCGGAGAAGC CACCTGCACT GCGGAGCTCA TCGTGGAGAC CATGGAGGAA 
GGGGAAGGGG AGGAGGGGGG AGAGGAGGAG GAGGAGGAGG AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( NM_139300.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_647461.3 Mus musculus myosin, light polypeptide kinase (Mylk), mRNA.

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CloneID	OMu20865
Clone ID Related Accession (Same CDS sequence)	NM_139300.3
Accession Version	NM_139300.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase, smooth muscle
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT010574.8, AK161028.1, AC154813.2, BC030478.1, AK078556.1 and AC110166.17. On Feb 24, 2007 this sequence version replaced NM_139300.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849388 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGGCC AGTTACCTGG GAAGACCATG GGGGACGTGA AACTGTTTGC CTCGTCACAC 
ATGTCTAAAA CCTCCCACAG TGTGGATCCC TCGAAGGTCA GCTCCATGCC TCTCACCGAG 
GCCCCAGCTT TCATTCTGCC CCCTCGGAAC CTCTGCGTCA AAGAAGGAGC CACAGCCAAG 
TTCGAAGGGA GGGTCCGGGG CTACCCAGAG CCCCAGGTGA CATGGCACAG AAAAGGGCAA 
GCCATCACCA ATGGTGGCCG CTTCCTGCTG GACTGTGGAG TGCGGGGGAC TTTTAGTCTT 
GTCATCCACA CTGTCCGCGA GGAGGACAAG GGCAAATACA CCTGTGAAGC TAGCAATGGC 
AGTGGCGCCC GCCAGGTGAC CGTGGAGCTG ACTGTGGAAG GGAATTCTAT GAAGAAGCGT 
GATCAGCCTG TTCTTTCTAA AGCCTCAGGA TTTCCAGGGG AGACTCGTCC TAGCATCTGG 
GGTGAGTGCC CACCAAAGTT TGCCACCAAG CTGGGCAGAG CTGTGGTCAA AGAAGGGCAG 
ATGGGGCGAT TCTCCTGCAA GATCACTGGC CGGCCTCCAC CACAGGTCAC CTGGCTCAAG 
GGAAATGTCC CGTTGCAGCC AAGTGCCCGT GTGTCAATGT CAGAGAAGAA TGGCATGCAG 
ATCCTGGAGA TCCGTGGGGT CACTCGGGAT GACCTGGGCG TGTACACCTG CATGGTGGTG 
AATGGGTCAG GGAAAGCCTC CATGTCGGCG GAGCTCTCCA TCCCAGGTTT GGACAATGCC 
GCTAGGTTGG CTGTGAGAGG AACCAAAGCC CCCAGTCCAG ACATCAGGAA GGAGGTGACC 
AATGGAGTCT CCAAAGACCC AGAGACTGTG GCTGAAAGTA AGAACTGTCC AAGTCCCCAG 
AGGAGTGGCT CCTCAGCCCG GGCCACCAAC AGTCACCTCA AGTCCCCGCA GGAGCCCAAG 
CCAAAGCTGT GTGAGGATGC TCCCAGAAAG GTCCCGCAGT CCTCCATCCT GCAGAAGTCC 
ACCAGCACCA TCACTCTGCA GGCCTTGAAA GTCCAGCCAG AAGCGAGAGT ACCAGCCATA 
GGGTCCTTCT CTCCTGGGGA AGACAGGAAG AGTCTGGCTG CCCCTCAGCA AGCCACACTC 
CCTACCAGAC AGTCTAGCCT GGGAGGTTCC GTTGGGAACA AGTTTGTTAC CGGGAACATC 
CCCAGAGAGA GCCAAAGGGA GTCAACATTC CCCAGATTTG AGAGTCAGCC CCAAAGCCAG 
GAAGTCACTG AAGGTCAGAC AGTCAAGTTC ATCTGCGAGG TTTCAGGTAT ACCAAAGCCT 
GACGTGGGCT GGTTCCTGGA AGGCATCCCC GTGAGGAGAC GGGAAGGCAT CACTGAGGTT 
TATGAAGATG GTGTATCCCA TCACCTGTGC CTGCTGAGAG CCCGGACGAG AGACAGTGGG 
AGGTACAGCT GCACAGCATC CAATAGCCTT GGCCAGGTGT CCTGCAGCTG GAGCCTGCTG 
GTGGACCGGC CGAACTTGGC ACAGACAGCC CCTTCATTCT CTAGTGTTCT GAAGGACAGT 
GTTGTCATTG AGGGCCAGGA TTTTGTGCTA CGGTGCTCAG TGCAGGGGAC TCCAGCACCC 
CGGGTCACGT GGCTGCTCAA TGGACAGCCC ATCCAGTTTG CTCACTCCAT CTGTGAGGCA 
GGCGTGGCTG AGCTCCACAT CCAGGATGCC CTGCCAGAGG ACCGTGGCAC CTACACCTGC 
CTGGCTGAGA ATGCCATGGG GCAGGTGTCC TGCAGCGCCA CTGTCACCGT CCAAGAGAAG 
AAAGGTGAAG GAGAGAGGGA ACACCGTCTG TCCCCAGCTC GCAGCAAGCC CATCGCACCC 
ATCTTCCTGC AGGGCCTCTC CGACCTCAAA GTCATGGATG GAAGCCAGGT CACTATGACA 
GTCCAGGTGT CAGGGAACCC ACCTCCGGAA GTCATCTGGC TTCACGACGG GAATGAGATT 
CAGGAGTCCG AGGACTTCCA CTTCGAGCAG AAGGGTGGCT GGCACAGCCT GTGTATCCAG 
GAGGTGTTCC CGGAGGACAC GGGCACGTAC ACCTGTGAGG CCTGGAACAG TGCCGGGGAG 
GTCCGCACCC GGGCCGTCCT CACCGTGCAA GAGCCTCATG ATGGCACCCA GCCCTGGTTC 
ATCAGCAAGC CTCGTTCGGT GACAGCCACC CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC 
ATAGCTGGAG ACCCCTTTCC CACCGTGCAC TGGCTCCGGG ATGGCAGAGC CCTCTCCAAG 
GACAGTGGCC ACTTTGAGCT GCTCCAGAAT GAGGACGTGT TCACCCTGGT TCTAAAGAAC 
GTGCAGCCCT GGCATGCCGG CCAGTATGAG ATTCTGCTCA AGAACCGGGT TGGCGAGTGT 
AGTTGCCAAG TGTCACTGAT GCTTCACAAC AGCCCTTCCA GAGCCCCACC ACGGGGGCGG 
GAGCCTGCCA GCTGCGAGGG CCTCTGTGGT GGTGGAGGAG TTGGTGCTCA TGGTGATGGT 
GACCGTCATG GGACCCTGAG GCCCTGCTGG CCAGCAAGAG GGCAAGGTTG GCCTGAGGAG 
GAAGACGGAG AAGACGTGAG GGGGCTGTTG AAGAGACGGG TGGAGACCAG GCTGCACACA 
GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG CAGCTGGATT TTCGAGACCT CCTGGGAAAG 
AAGGTGAGCA CCAAGACCGT CTCAGAAGAC GACCTGAAGG ACATCCCAGC CGAGCAGATG 
GATTTCCGCG CCAACCTTCA GCGGCAAGTG AAGCCAAAGA CCATATCCGA GGAAGAGAGG 
AAGGTGCATA GCCCTCAGCA GGTTGACTTC CGGTCTGTCC TCGCCAAGAA GGGGACCCCT 
AAGACCCCCG TTCCTGAGAA GGCACCTCCA AAAGCTGCCA CCCCAGACTT TCGCTCTGTG 
CTAGGTGGCA AGAAGAAGTC ACCCTCGGAG AATGGGGGCA ACAGCGCTGA GGTCTTGAAT 
GTCAAGGCTG GGGAAAGCCC CACACCTGCA GGCGATGCGC AGGCCATCGG GGCCCTGAAA 
CCCGTAGGCA ATGCCAAGCC TGCTGAGACC CCGAAACCTA TAGGTAATGC CAAGCCTACC 
GAGACCCTGA AGCCTGTGGG CAACACCAAG CCTGCCGAGA CCCTGAAACC CATAGCCAAC 
GCACAGCCTT CAGGGTCCCT GAAACCTGTA ACCAACGCAC AACCTGCTGA GCCCCAGAAG 
CCTGTGGGCA ATGCCAAGTC TGCTGAGACC TCGAAACCAG CTGGGAAAGA AGAAGTCAAG 
GAGGTAAAGA ATGATGTGAA CTGTAAGAAA GGGCAGGTCG GAGCCACGGG CAATGAAAAG 
CGACCCGAGA GCCAAGGCTC GGCCCCAGTT TTCAAGGAAA AGCTACAAGA TGTCCATGTG 
GCAGAGGGGG AGAAGCTGCT ACTCCAATGC CAGGTGATCT CTGATCCCCC CGCCACTGTC 
ACCTGGTCGC TGAATGGGAA GACACTCAAG ACCACAAAAT TCATCGTCCT CGCCCAAGAA 
GGCTCACGTT TCTCTGTCTC CATCGAGAAG GCACTGCCTG AGGACCGAGG TTTGTACAAG 
TGTGTAGCCA AGAACAGCGC TGGCCAGGCA GAGTGTTCCT GCCAAGTCAC TGTGGACGAT 
GCTCAGACCA GTGAGAACAC CAAGGCCCCG GAGATGAAAT CCCGGAGACC CAAGAGCTCT 
CTTCCCCCTG TGCTAGGAAC AGAGAGCGAC GCAACTGTGA AAAAGAAACC TGCCCCCAAG 
ACACCCACAA AAGCAGCTAT GCCCCCTCAA ATCATCCAGT TCCCCGAGGA CCAGAAGGTG 
CGCGCAGGAG AACCTGTGGA GCTGTTTGGG AAAGTGGCTG GCACCCAGCC CATCACCTGC 
AAGTGGATGA AGTTCCGAAA GCAGATCCAG GAGAGCGAGC ATATCAAGGT GGAGAATGGC 
GAGAGCGGCA GCAAGCTCAC CATCCTGGCC GCGCGGCAGG AGCACTGTGG CTGCTACACA 
CTGGTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAAG TCAACCTCAC GGTCGTGGAT 
AAGCCAGACC CCCCGGCTGG CACACCTTGT GCTTCTGACA TTCGGAGTTC CTCACTGACC 
CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAATGTGGAG 
ATCTGGGACA CAGAGGACAA GGTGTGGAAA GAGCTAGCCA CGTGCCGCAG CACCTCTTTT 
AATGTCCAAG ACCTGCTGCC TGACCGCGAG TACAAATTCC GAGTGCGTGC AGTCAACGTC 
TATGGAACCA GCGAGCCAAG CCAAGAGTCC GAGCTCACAG CTGTGGGAGA GAAACCTGAA 
GAGCCAAAGG ATGAAGTGGA AGTGTCCGAT GATGACGAAA AGGAACCTGA GGTTGATTAC 
CGGACGGTGA CTGTTAACAC TGAACAGAAA GTGTCTGATG TGTATGACAT TGAAGAGCGA 
CTAGGATCTG GGAAGTTTGG ACAAGTCTTC CGACTTGTGG AAAAGAAAAC TGGAAAAATC 
TGGGCAGGGA AATTTTTCAA GGCCTATTCC GCCAAGGAGA AAGACAACAT CCGTCAGGAG 
ATCAGCATCA TGAACTGCCT CCACCACCCC AAGCTGGTCC AGTGTGTGGA TGCCTTCGAA 
GAAAAGGCCA ACATTGTCAT GGTTCTGGAG ATCGTGTCCG GGGGTGAGCT GTTTGAGCGT 
ATCATTGACG AGGACTTCGA GCTGACGGAG CGGGAGTGTA TCAAGTACAT GAGGCAGATC 
TCGGAAGGGG TGGAGTACAT CCACAAGCAG GGCATTGTGC ACCTGGACCT CAAGCCCGAG 
AACATCATGT GTGTCAACAA GACAGGCACC AGGATCAAGC TCATTGACTT CGGCCTGGCA 
CGGAGACTCG AGAACGCTGG GTCTCTGAAG GTCCTCTTTG GGACCCCAGA GTTTGTGGCT 
CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC 
ATCTGCTATA TTCTGGTCAG CGGACTTTCT CCCTTCATGG GTGACAATGA TAACGAGACC 
TTAGCCAACG TCACTTCAGC CACCTGGGAC TTCGATGACG AGGCATTCGA TGAGATCTCC 
GATGACGCAA AGGATTTCAT CAGTAACTTG CTGAAGAAAG ATATGAAAAA CCGTCTGGAC 
TGCACCCAGT GCCTTCAGCA CCCATGGCTG ATGAAAGACA CCAAGAACAT GGAGGCCAAG 
AAGCTGTCCA AGGACCGGAT GAAGAAATAC ATGGCAAGAA GGAAGTGGCA GAAAACGGGC 
AATGCTGTGA GAGCCATCGG GAGACTGTCC TCTATGGCAA TGATCTCAGG GCTCAGTGGC 
AGGAAATCTT CAACAGGGTC ACCAACCAGC CCAATCAATG CGGAGAAACT AGAGTCTGAA 
GACGATGTCT CCCAAGCTTT CCTGGAGGCT GTTGCTGAGG AAAAGCCCCA TGTGAAACCT 
TATTTCTCTA AGACCATCCG TGATCTGGAA GTCGTGGAGG GAAGCGCTGC TAGATTTGAC 
TGCAAGATTG AAGGATACCC AGACCCTGAG GTGGTCTGGT TCAAGGATGA CCAATCAATC 
AGGGAGTCCC GCCACTTCCA GATAGACTAC GACGAAGACG GCAACTGCTC GCTCATCATC 
AGCGACGTCT GTGGGGATGA TGACGCCAAG TACACCTGCA AGGCTGTCAA CAGCCTCGGA 
GAAGCCACCT GCACTGCGGA GCTCATCGTG GAGACCATGG AGGAAGGGGA AGGGGAGGAG 
GGGGGAGAGG AGGAGGAGGA GGAGGAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647461.3
CDS	55..5907
Translation	MSGQLPGKTMGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRNLCVKEGATAKFEGRVRGYPEPQVTWHRKGQAITNGGRFLLDCGVRGTFSLVIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKRDQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSAELSIPGLDNAARLAVRGTKAPSPDIRKEVTNGVSKDPETVAESKNCPSPQRSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALKVQPEARVPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSVGNKFVTGNIPRESQRESTFPRFESQPQSQEVTEGQTVKFICEVSGIPKPDVGWFLEGIPVRRREGITEVYEDGVSHHLCLLRARTRDSGRYSCTASNSLGQVSCSWSLLVDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQPIQFAHSICEAGVAELHIQDALPEDRGTYTCLAENAMGQVSCSATVTVQEKKGEGEREHRLSPARSKPIAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVHWLRDGRALSKDSGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDGDRHGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKAPPKAATPDFRSVLGGKKKSPSENGGNSAEVLNVKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTKPAETLKPIANAQPSGSLKPVTNAQPAEPQKPVGNAKSAETSKPAGKEEVKEVKNDVNCKKGQVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVISDPPATVTWSLNGKTLKTTKFIVLAQEGSRFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE

Target ORF information:

RefSeq Version	NM_139300.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide kinase (Mylk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139300.3

ORF Insert Sequence:

ATGTCTGGCC AGTTACCTGG GAAGACCATG GGGGACGTGA AACTGTTTGC CTCGTCACAC 
ATGTCTAAAA CCTCCCACAG TGTGGATCCC TCGAAGGTCA GCTCCATGCC TCTCACCGAG 
GCCCCAGCTT TCATTCTGCC CCCTCGGAAC CTCTGCGTCA AAGAAGGAGC CACAGCCAAG 
TTCGAAGGGA GGGTCCGGGG CTACCCAGAG CCCCAGGTGA CATGGCACAG AAAAGGGCAA 
GCCATCACCA ATGGTGGCCG CTTCCTGCTG GACTGTGGAG TGCGGGGGAC TTTTAGTCTT 
GTCATCCACA CTGTCCGCGA GGAGGACAAG GGCAAATACA CCTGTGAAGC TAGCAATGGC 
AGTGGCGCCC GCCAGGTGAC CGTGGAGCTG ACTGTGGAAG GGAATTCTAT GAAGAAGCGT 
GATCAGCCTG TTCTTTCTAA AGCCTCAGGA TTTCCAGGGG AGACTCGTCC TAGCATCTGG 
GGTGAGTGCC CACCAAAGTT TGCCACCAAG CTGGGCAGAG CTGTGGTCAA AGAAGGGCAG 
ATGGGGCGAT TCTCCTGCAA GATCACTGGC CGGCCTCCAC CACAGGTCAC CTGGCTCAAG 
GGAAATGTCC CGTTGCAGCC AAGTGCCCGT GTGTCAATGT CAGAGAAGAA TGGCATGCAG 
ATCCTGGAGA TCCGTGGGGT CACTCGGGAT GACCTGGGCG TGTACACCTG CATGGTGGTG 
AATGGGTCAG GGAAAGCCTC CATGTCGGCG GAGCTCTCCA TCCCAGGTTT GGACAATGCC 
GCTAGGTTGG CTGTGAGAGG AACCAAAGCC CCCAGTCCAG ACATCAGGAA GGAGGTGACC 
AATGGAGTCT CCAAAGACCC AGAGACTGTG GCTGAAAGTA AGAACTGTCC AAGTCCCCAG 
AGGAGTGGCT CCTCAGCCCG GGCCACCAAC AGTCACCTCA AGTCCCCGCA GGAGCCCAAG 
CCAAAGCTGT GTGAGGATGC TCCCAGAAAG GTCCCGCAGT CCTCCATCCT GCAGAAGTCC 
ACCAGCACCA TCACTCTGCA GGCCTTGAAA GTCCAGCCAG AAGCGAGAGT ACCAGCCATA 
GGGTCCTTCT CTCCTGGGGA AGACAGGAAG AGTCTGGCTG CCCCTCAGCA AGCCACACTC 
CCTACCAGAC AGTCTAGCCT GGGAGGTTCC GTTGGGAACA AGTTTGTTAC CGGGAACATC 
CCCAGAGAGA GCCAAAGGGA GTCAACATTC CCCAGATTTG AGAGTCAGCC CCAAAGCCAG 
GAAGTCACTG AAGGTCAGAC AGTCAAGTTC ATCTGCGAGG TTTCAGGTAT ACCAAAGCCT 
GACGTGGGCT GGTTCCTGGA AGGCATCCCC GTGAGGAGAC GGGAAGGCAT CACTGAGGTT 
TATGAAGATG GTGTATCCCA TCACCTGTGC CTGCTGAGAG CCCGGACGAG AGACAGTGGG 
AGGTACAGCT GCACAGCATC CAATAGCCTT GGCCAGGTGT CCTGCAGCTG GAGCCTGCTG 
GTGGACCGGC CGAACTTGGC ACAGACAGCC CCTTCATTCT CTAGTGTTCT GAAGGACAGT 
GTTGTCATTG AGGGCCAGGA TTTTGTGCTA CGGTGCTCAG TGCAGGGGAC TCCAGCACCC 
CGGGTCACGT GGCTGCTCAA TGGACAGCCC ATCCAGTTTG CTCACTCCAT CTGTGAGGCA 
GGCGTGGCTG AGCTCCACAT CCAGGATGCC CTGCCAGAGG ACCGTGGCAC CTACACCTGC 
CTGGCTGAGA ATGCCATGGG GCAGGTGTCC TGCAGCGCCA CTGTCACCGT CCAAGAGAAG 
AAAGGTGAAG GAGAGAGGGA ACACCGTCTG TCCCCAGCTC GCAGCAAGCC CATCGCACCC 
ATCTTCCTGC AGGGCCTCTC CGACCTCAAA GTCATGGATG GAAGCCAGGT CACTATGACA 
GTCCAGGTGT CAGGGAACCC ACCTCCGGAA GTCATCTGGC TTCACGACGG GAATGAGATT 
CAGGAGTCCG AGGACTTCCA CTTCGAGCAG AAGGGTGGCT GGCACAGCCT GTGTATCCAG 
GAGGTGTTCC CGGAGGACAC GGGCACGTAC ACCTGTGAGG CCTGGAACAG TGCCGGGGAG 
GTCCGCACCC GGGCCGTCCT CACCGTGCAA GAGCCTCATG ATGGCACCCA GCCCTGGTTC 
ATCAGCAAGC CTCGTTCGGT GACAGCCACC CTGGGCCAGA GTGTCCTCAT CTCCTGCGCC 
ATAGCTGGAG ACCCCTTTCC CACCGTGCAC TGGCTCCGGG ATGGCAGAGC CCTCTCCAAG 
GACAGTGGCC ACTTTGAGCT GCTCCAGAAT GAGGACGTGT TCACCCTGGT TCTAAAGAAC 
GTGCAGCCCT GGCATGCCGG CCAGTATGAG ATTCTGCTCA AGAACCGGGT TGGCGAGTGT 
AGTTGCCAAG TGTCACTGAT GCTTCACAAC AGCCCTTCCA GAGCCCCACC ACGGGGGCGG 
GAGCCTGCCA GCTGCGAGGG CCTCTGTGGT GGTGGAGGAG TTGGTGCTCA TGGTGATGGT 
GACCGTCATG GGACCCTGAG GCCCTGCTGG CCAGCAAGAG GGCAAGGTTG GCCTGAGGAG 
GAAGACGGAG AAGACGTGAG GGGGCTGTTG AAGAGACGGG TGGAGACCAG GCTGCACACA 
GAAGAGGCCA TCCGCCAGCA GGAGGTGGGG CAGCTGGATT TTCGAGACCT CCTGGGAAAG 
AAGGTGAGCA CCAAGACCGT CTCAGAAGAC GACCTGAAGG ACATCCCAGC CGAGCAGATG 
GATTTCCGCG CCAACCTTCA GCGGCAAGTG AAGCCAAAGA CCATATCCGA GGAAGAGAGG 
AAGGTGCATA GCCCTCAGCA GGTTGACTTC CGGTCTGTCC TCGCCAAGAA GGGGACCCCT 
AAGACCCCCG TTCCTGAGAA GGCACCTCCA AAAGCTGCCA CCCCAGACTT TCGCTCTGTG 
CTAGGTGGCA AGAAGAAGTC ACCCTCGGAG AATGGGGGCA ACAGCGCTGA GGTCTTGAAT 
GTCAAGGCTG GGGAAAGCCC CACACCTGCA GGCGATGCGC AGGCCATCGG GGCCCTGAAA 
CCCGTAGGCA ATGCCAAGCC TGCTGAGACC CCGAAACCTA TAGGTAATGC CAAGCCTACC 
GAGACCCTGA AGCCTGTGGG CAACACCAAG CCTGCCGAGA CCCTGAAACC CATAGCCAAC 
GCACAGCCTT CAGGGTCCCT GAAACCTGTA ACCAACGCAC AACCTGCTGA GCCCCAGAAG 
CCTGTGGGCA ATGCCAAGTC TGCTGAGACC TCGAAACCAG CTGGGAAAGA AGAAGTCAAG 
GAGGTAAAGA ATGATGTGAA CTGTAAGAAA GGGCAGGTCG GAGCCACGGG CAATGAAAAG 
CGACCCGAGA GCCAAGGCTC GGCCCCAGTT TTCAAGGAAA AGCTACAAGA TGTCCATGTG 
GCAGAGGGGG AGAAGCTGCT ACTCCAATGC CAGGTGATCT CTGATCCCCC CGCCACTGTC 
ACCTGGTCGC TGAATGGGAA GACACTCAAG ACCACAAAAT TCATCGTCCT CGCCCAAGAA 
GGCTCACGTT TCTCTGTCTC CATCGAGAAG GCACTGCCTG AGGACCGAGG TTTGTACAAG 
TGTGTAGCCA AGAACAGCGC TGGCCAGGCA GAGTGTTCCT GCCAAGTCAC TGTGGACGAT 
GCTCAGACCA GTGAGAACAC CAAGGCCCCG GAGATGAAAT CCCGGAGACC CAAGAGCTCT 
CTTCCCCCTG TGCTAGGAAC AGAGAGCGAC GCAACTGTGA AAAAGAAACC TGCCCCCAAG 
ACACCCACAA AAGCAGCTAT GCCCCCTCAA ATCATCCAGT TCCCCGAGGA CCAGAAGGTG 
CGCGCAGGAG AACCTGTGGA GCTGTTTGGG AAAGTGGCTG GCACCCAGCC CATCACCTGC 
AAGTGGATGA AGTTCCGAAA GCAGATCCAG GAGAGCGAGC ATATCAAGGT GGAGAATGGC 
GAGAGCGGCA GCAAGCTCAC CATCCTGGCC GCGCGGCAGG AGCACTGTGG CTGCTACACA 
CTGGTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAAG TCAACCTCAC GGTCGTGGAT 
AAGCCAGACC CCCCGGCTGG CACACCTTGT GCTTCTGACA TTCGGAGTTC CTCACTGACC 
CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAATGTGGAG 
ATCTGGGACA CAGAGGACAA GGTGTGGAAA GAGCTAGCCA CGTGCCGCAG CACCTCTTTT 
AATGTCCAAG ACCTGCTGCC TGACCGCGAG TACAAATTCC GAGTGCGTGC AGTCAACGTC 
TATGGAACCA GCGAGCCAAG CCAAGAGTCC GAGCTCACAG CTGTGGGAGA GAAACCTGAA 
GAGCCAAAGG ATGAAGTGGA AGTGTCCGAT GATGACGAAA AGGAACCTGA GGTTGATTAC 
CGGACGGTGA CTGTTAACAC TGAACAGAAA GTGTCTGATG TGTATGACAT TGAAGAGCGA 
CTAGGATCTG GGAAGTTTGG ACAAGTCTTC CGACTTGTGG AAAAGAAAAC TGGAAAAATC 
TGGGCAGGGA AATTTTTCAA GGCCTATTCC GCCAAGGAGA AAGACAACAT CCGTCAGGAG 
ATCAGCATCA TGAACTGCCT CCACCACCCC AAGCTGGTCC AGTGTGTGGA TGCCTTCGAA 
GAAAAGGCCA ACATTGTCAT GGTTCTGGAG ATCGTGTCCG GGGGTGAGCT GTTTGAGCGT 
ATCATTGACG AGGACTTCGA GCTGACGGAG CGGGAGTGTA TCAAGTACAT GAGGCAGATC 
TCGGAAGGGG TGGAGTACAT CCACAAGCAG GGCATTGTGC ACCTGGACCT CAAGCCCGAG 
AACATCATGT GTGTCAACAA GACAGGCACC AGGATCAAGC TCATTGACTT CGGCCTGGCA 
CGGAGACTCG AGAACGCTGG GTCTCTGAAG GTCCTCTTTG GGACCCCAGA GTTTGTGGCT 
CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC 
ATCTGCTATA TTCTGGTCAG CGGACTTTCT CCCTTCATGG GTGACAATGA TAACGAGACC 
TTAGCCAACG TCACTTCAGC CACCTGGGAC TTCGATGACG AGGCATTCGA TGAGATCTCC 
GATGACGCAA AGGATTTCAT CAGTAACTTG CTGAAGAAAG ATATGAAAAA CCGTCTGGAC 
TGCACCCAGT GCCTTCAGCA CCCATGGCTG ATGAAAGACA CCAAGAACAT GGAGGCCAAG 
AAGCTGTCCA AGGACCGGAT GAAGAAATAC ATGGCAAGAA GGAAGTGGCA GAAAACGGGC 
AATGCTGTGA GAGCCATCGG GAGACTGTCC TCTATGGCAA TGATCTCAGG GCTCAGTGGC 
AGGAAATCTT CAACAGGGTC ACCAACCAGC CCAATCAATG CGGAGAAACT AGAGTCTGAA 
GACGATGTCT CCCAAGCTTT CCTGGAGGCT GTTGCTGAGG AAAAGCCCCA TGTGAAACCT 
TATTTCTCTA AGACCATCCG TGATCTGGAA GTCGTGGAGG GAAGCGCTGC TAGATTTGAC 
TGCAAGATTG AAGGATACCC AGACCCTGAG GTGGTCTGGT TCAAGGATGA CCAATCAATC 
AGGGAGTCCC GCCACTTCCA GATAGACTAC GACGAAGACG GCAACTGCTC GCTCATCATC 
AGCGACGTCT GTGGGGATGA TGACGCCAAG TACACCTGCA AGGCTGTCAA CAGCCTCGGA 
GAAGCCACCT GCACTGCGGA GCTCATCGTG GAGACCATGG AGGAAGGGGA AGGGGAGGAG 
GGGGGAGAGG AGGAGGAGGA GGAGGAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_006521703.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521766.1 Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X2, mRNA.

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CloneID	OMu51811
Clone ID Related Accession (Same CDS sequence)	XM_006521703.3 , XM_006521702.1
Accession Version	XM_006521703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGTT TGCCTCGTCA CACATGTCTA AAACCTCCCA CAGTGTGGAT 
CCCTCGAAGG TCAGCTCCAT GCCTCTCACC GAGGCCCCAG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACAGCC AAGTTCGAAG GGAGGGTCCG GGGCTACCCA 
GAGCCCCAGG TGACATGGCA CAGAAAAGGG CAAGCCATCA CCAATGGTGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTTAGT CTTGTCATCC ACACTGTCCG CGAGGAGGAC 
AAGGGCAAAT ACACCTGTGA AGCTAGCAAT GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGAATTC TATGAAGAAG CGTGATCAGC CTGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCACCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTCTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGTGTGTCAA TGTCAGAGAA GAATGGCATG CAGATCCTGG AGATCCGTGG GGTCACTCGG 
GATGACCTGG GCGTGTACAC CTGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCG 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCGCTAGGT TGGCTGTGAG AGGAACCAAA 
GCCCCCAGTC CAGACATCAG GAAGGAGGTG ACCAATGGAG TCTCCAAAGA CCCAGAGACT 
GTGGCTGAAA GTAAGAACTG TCCAAGTCCC CAGAGGAGTG GCTCCTCAGC CCGGGCCACC 
AACAGTCACC TCAAGTCCCC GCAGGAGCCC AAGCCAAAGC TGTGTGAGGA TGCTCCCAGA 
AAGGTCCCGC AGTCCTCCAT CCTGCAGAAG TCCACCAGCA CCATCACTCT GCAGGCCTTG 
AAAGTCCAGC CAGAAGCGAG AGTACCAGCC ATAGGGTCCT TCTCTCCTGG GGAAGACAGG 
AAGAGTCTGG CTGCCCCTCA GCAAGCCACA CTCCCTACCA GACAGTCTAG CCTGGGAGGT 
TCCGTTGGGA ACAAGTTTGT TACCGGGAAC ATCCCCAGAG AGAGCCAAAG GGAGTCAACA 
TTCCCCAGAT TTGAGAGTCA GCCCCAAAGC CAGGAAGTCA CTGAAGGTCA GACAGTCAAG 
TTCATCTGCG AGGTTTCAGG TATACCAAAG CCTGACGTGG GCTGGTTCCT GGAAGGCATC 
CCCGTGAGGA GACGGGAAGG CATCACTGAG GTTTATGAAG ATGGTGTATC CCATCACCTG 
TGCCTGCTGA GAGCCCGGAC GAGAGACAGT GGGAGGTACA GCTGCACAGC ATCCAATAGC 
CTTGGCCAGG TGTCCTGCAG CTGGAGCCTG CTGGTGGACC GGCCGAACTT GGCACAGACA 
GCCCCTTCAT TCTCTAGTGT TCTGAAGGAC AGTGTTGTCA TTGAGGGCCA GGATTTTGTG 
CTACGGTGCT CAGTGCAGGG GACTCCAGCA CCCCGGGTCA CGTGGCTGCT CAATGGACAG 
CCCATCCAGT TTGCTCACTC CATCTGTGAG GCAGGCGTGG CTGAGCTCCA CATCCAGGAT 
GCCCTGCCAG AGGACCGTGG CACCTACACC TGCCTGGCTG AGAATGCCAT GGGGCAGGTG 
TCCTGCAGCG CCACTGTCAC CGTCCAAGAG AAGAAAGGTG AAGGAGAGAG GGAACACCGT 
CTGTCCCCAG CTCGCAGCAA GCCCATCGCA CCCATCTTCC TGCAGGGCCT CTCCGACCTC 
AAAGTCATGG ATGGAAGCCA GGTCACTATG ACAGTCCAGG TGTCAGGGAA CCCACCTCCG 
GAAGTCATCT GGCTTCACGA CGGGAATGAG ATTCAGGAGT CCGAGGACTT CCACTTCGAG 
CAGAAGGGTG GCTGGCACAG CCTGTGTATC CAGGAGGTGT TCCCGGAGGA CACGGGCACG 
TACACCTGTG AGGCCTGGAA CAGTGCCGGG GAGGTCCGCA CCCGGGCCGT CCTCACCGTG 
CAAGAGCCTC ATGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCTCGTTC GGTGACAGCC 
ACCCTGGGCC AGAGTGTCCT CATCTCCTGC GCCATAGCTG GAGACCCCTT TCCCACCGTG 
CACTGGCTCC GGGATGGCAG AGCCCTCTCC AAGGACAGTG GCCACTTTGA GCTGCTCCAG 
AATGAGGACG TGTTCACCCT GGTTCTAAAG AACGTGCAGC CCTGGCATGC CGGCCAGTAT 
GAGATTCTGC TCAAGAACCG GGTTGGCGAG TGTAGTTGCC AAGTGTCACT GATGCTTCAC 
AACAGCCCTT CCAGAGCCCC ACCACGGGGG CGGGAGCCTG CCAGCTGCGA GGGCCTCTGT 
GGTGGTGGAG GAGTTGGTGC TCATGGTGAT GGTGACCGTC ATGGGACCCT GAGGCCCTGC 
TGGCCAGCAA GAGGGCAAGG TTGGCCTGAG GAGGAAGACG GAGAAGACGT GAGGGGGCTG 
TTGAAGAGAC GGGTGGAGAC CAGGCTGCAC ACAGAAGAGG CCATCCGCCA GCAGGAGGTG 
GGGCAGCTGG ATTTTCGAGA CCTCCTGGGA AAGAAGGTGA GCACCAAGAC CGTCTCAGAA 
GACGACCTGA AGGACATCCC AGCCGAGCAG ATGGATTTCC GCGCCAACCT TCAGCGGCAA 
GTGAAGCCAA AGACCATATC CGAGGAAGAG AGGAAGGTGC ATAGCCCTCA GCAGGTTGAC 
TTCCGGTCTG TCCTCGCCAA GAAGGGGACC CCTAAGACCC CCGTTCCTGA GAAGGCACCT 
CCAAAAGCTG CCACCCCAGA CTTTCGCTCT GTGCTAGGTG GCAAGAAGAA GTCACCCTCG 
GAGAATGGGG GCAACAGCGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACACCT 
GCAGGCGATG CGCAGGCCAT CGGGGCCCTG AAACCCGTAG GCAATGCCAA GCCTGCTGAG 
ACCCCGAAAC CTATAGGTAA TGCCAAGCCT ACCGAGACCC TGAAGCCTGT GGGCAACACC 
AAGCCTGCCG AGACCCTGAA ACCCATAGCC AACGCACAGC CTTCAGGGTC CCTGAAACCT 
GTAACCAACG CACAACCTGC TGAGCCCCAG AAGCCTGTGG GCAATGCCAA GTCTGCTGAG 
ACCTCGAAAC CAGCTGGGAA AGAAGAAGTC AAGGAGGTAA AGAATGATGT GAACTGTAAG 
AAAGGGCAGG TCGGAGCCAC GGGCAATGAA AAGCGACCCG AGAGCCAAGG CTCGGCCCCA 
GTTTTCAAGG AAAAGCTACA AGATGTCCAT GTGGCAGAGG GGGAGAAGCT GCTACTCCAA 
TGCCAGGTGA TCTCTGATCC CCCCGCCACT GTCACCTGGT CGCTGAATGG GAAGACACTC 
AAGACCACAA AATTCATCGT CCTCGCCCAA GAAGGCTCAC GTTTCTCTGT CTCCATCGAG 
AAGGCACTGC CTGAGGACCG AGGTTTGTAC AAGTGTGTAG CCAAGAACAG CGCTGGCCAG 
GCAGAGTGTT CCTGCCAAGT CACTGTGGAC GATGCTCAGA CCAGTGAGAA CACCAAGGCC 
CCGGAGATGA AATCCCGGAG ACCCAAGAGC TCTCTTCCCC CTGTGCTAGG AACAGAGAGC 
GACGCAACTG TGAAAAAGAA ACCTGCCCCC AAGACACCCA CAAAAGCAGC TATGCCCCCT 
CAAATCATCC AGTTCCCCGA GGACCAGAAG GTGCGCGCAG GAGAACCTGT GGAGCTGTTT 
GGGAAAGTGG CTGGCACCCA GCCCATCACC TGCAAGTGGA TGAAGTTCCG AAAGCAGATC 
CAGGAGAGCG AGCATATCAA GGTGGAGAAT GGCGAGAGCG GCAGCAAGCT CACCATCCTG 
GCCGCGCGGC AGGAGCACTG TGGCTGCTAC ACACTGGTGG TGGAGAACAA GCTGGGCAGC 
AGGCAGGCCC AAGTCAACCT CACGGTCGTG GATAAGCCAG ACCCCCCGGC TGGCACACCT 
TGTGCTTCTG ACATTCGGAG TTCCTCACTG ACCCTGTCCT GGTATGGCTC CTCATATGAT 
GGGGGCAGTG CTGTACAGTC CTACAATGTG GAGATCTGGG ACACAGAGGA CAAGGTGTGG 
AAAGAGCTAG CCACGTGCCG CAGCACCTCT TTTAATGTCC AAGACCTGCT GCCTGACCGC 
GAGTACAAAT TCCGAGTGCG TGCAGTCAAC GTCTATGGAA CCAGCGAGCC AAGCCAAGAG 
TCCGAGCTCA CAGCTGTGGG AGAGAAACCT GAAGAGCCAA AGGATGAAGT GGAAGTGTCC 
GATGATGACG AAAAGGAACC TGAGGTTGAT TACCGGACGG TGACTGTTAA CACTGAACAG 
AAAGTGTCTG ATGTGTATGA CATTGAAGAG CGACTAGGAT CTGGGAAGTT TGGACAAGTC 
TTCCGACTTG TGGAAAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTTTT CAAGGCCTAT 
TCCGCCAAGG AGAAAGACAA CATCCGTCAG GAGATCAGCA TCATGAACTG CCTCCACCAC 
CCCAAGCTGG TCCAGTGTGT GGATGCCTTC GAAGAAAAGG CCAACATTGT CATGGTTCTG 
GAGATCGTGT CCGGGGGTGA GCTGTTTGAG CGTATCATTG ACGAGGACTT CGAGCTGACG 
GAGCGGGAGT GTATCAAGTA CATGAGGCAG ATCTCGGAAG GGGTGGAGTA CATCCACAAG 
CAGGGCATTG TGCACCTGGA CCTCAAGCCC GAGAACATCA TGTGTGTCAA CAAGACAGGC 
ACCAGGATCA AGCTCATTGA CTTCGGCCTG GCACGGAGAC TCGAGAACGC TGGGTCTCTG 
AAGGTCCTCT TTGGGACCCC AGAGTTTGTG GCTCCTGAAG TGATCAACTA TGAGCCCATC 
GGCTACGCCA CAGACATGTG GAGCATCGGG GTCATCTGCT ATATTCTGGT CAGCGGACTT 
TCTCCCTTCA TGGGTGACAA TGATAACGAG ACCTTAGCCA ACGTCACTTC AGCCACCTGG 
GACTTCGATG ACGAGGCATT CGATGAGATC TCCGATGACG CAAAGGATTT CATCAGTAAC 
TTGCTGAAGA AAGATATGAA AAACCGTCTG GACTGCACCC AGTGCCTTCA GCACCCATGG 
CTGATGAAAG ACACCAAGAA CATGGAGGCC AAGAAGCTGT CCAAGGACCG GATGAAGAAA 
TACATGGCAA GAAGGAAGTG GCAGAAAACG GGCAATGCTG TGAGAGCCAT CGGGAGACTG 
TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CTTCAACAGG GTCACCAACC 
AGCCCAATCA ATGCGGAGAA ACTAGAGTCT GAAGACGATG TCTCCCAAGC TTTCCTGGAG 
GCTGTTGCTG AGGAAAAGCC CCATGTGAAA CCTTATTTCT CTAAGACCAT CCGTGATCTG 
GAAGTCGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT 
GAGGTGGTCT GGTTCAAGGA TGACCAATCA ATCAGGGAGT CCCGCCACTT CCAGATAGAC 
TACGACGAAG ACGGCAACTG CTCGCTCATC ATCAGCGACG TCTGTGGGGA TGATGACGCC 
AAGTACACCT GCAAGGCTGT CAACAGCCTC GGAGAAGCCA CCTGCACTGC GGAGCTCATC 
GTGGAGACCA TGGAGGAAGG GGAAGGGGAG GAGGGGGGAG AGGAGGAGGA GGAGGAGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521766.1
CDS	143..5968
Translation	MGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRNLCVKEGATAKFEGRVRGYPEPQVTWHRKGQAITNGGRFLLDCGVRGTFSLVIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKRDQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSAELSIPGLDNAARLAVRGTKAPSPDIRKEVTNGVSKDPETVAESKNCPSPQRSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALKVQPEARVPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSVGNKFVTGNIPRESQRESTFPRFESQPQSQEVTEGQTVKFICEVSGIPKPDVGWFLEGIPVRRREGITEVYEDGVSHHLCLLRARTRDSGRYSCTASNSLGQVSCSWSLLVDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQPIQFAHSICEAGVAELHIQDALPEDRGTYTCLAENAMGQVSCSATVTVQEKKGEGEREHRLSPARSKPIAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVHWLRDGRALSKDSGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDGDRHGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKAPPKAATPDFRSVLGGKKKSPSENGGNSAEVLNVKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTKPAETLKPIANAQPSGSLKPVTNAQPAEPQKPVGNAKSAETSKPAGKEEVKEVKNDVNCKKGQVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVISDPPATVTWSLNGKTLKTTKFIVLAQEGSRFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE

Target ORF information:

RefSeq Version	XM_006521703.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521703.3

ORF Insert Sequence:

ATGGGGGACG TGAAACTGTT TGCCTCGTCA CACATGTCTA AAACCTCCCA CAGTGTGGAT 
CCCTCGAAGG TCAGCTCCAT GCCTCTCACC GAGGCCCCAG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACAGCC AAGTTCGAAG GGAGGGTCCG GGGCTACCCA 
GAGCCCCAGG TGACATGGCA CAGAAAAGGG CAAGCCATCA CCAATGGTGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTTAGT CTTGTCATCC ACACTGTCCG CGAGGAGGAC 
AAGGGCAAAT ACACCTGTGA AGCTAGCAAT GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGAATTC TATGAAGAAG CGTGATCAGC CTGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCACCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTCTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGTGTGTCAA TGTCAGAGAA GAATGGCATG CAGATCCTGG AGATCCGTGG GGTCACTCGG 
GATGACCTGG GCGTGTACAC CTGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCG 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCGCTAGGT TGGCTGTGAG AGGAACCAAA 
GCCCCCAGTC CAGACATCAG GAAGGAGGTG ACCAATGGAG TCTCCAAAGA CCCAGAGACT 
GTGGCTGAAA GTAAGAACTG TCCAAGTCCC CAGAGGAGTG GCTCCTCAGC CCGGGCCACC 
AACAGTCACC TCAAGTCCCC GCAGGAGCCC AAGCCAAAGC TGTGTGAGGA TGCTCCCAGA 
AAGGTCCCGC AGTCCTCCAT CCTGCAGAAG TCCACCAGCA CCATCACTCT GCAGGCCTTG 
AAAGTCCAGC CAGAAGCGAG AGTACCAGCC ATAGGGTCCT TCTCTCCTGG GGAAGACAGG 
AAGAGTCTGG CTGCCCCTCA GCAAGCCACA CTCCCTACCA GACAGTCTAG CCTGGGAGGT 
TCCGTTGGGA ACAAGTTTGT TACCGGGAAC ATCCCCAGAG AGAGCCAAAG GGAGTCAACA 
TTCCCCAGAT TTGAGAGTCA GCCCCAAAGC CAGGAAGTCA CTGAAGGTCA GACAGTCAAG 
TTCATCTGCG AGGTTTCAGG TATACCAAAG CCTGACGTGG GCTGGTTCCT GGAAGGCATC 
CCCGTGAGGA GACGGGAAGG CATCACTGAG GTTTATGAAG ATGGTGTATC CCATCACCTG 
TGCCTGCTGA GAGCCCGGAC GAGAGACAGT GGGAGGTACA GCTGCACAGC ATCCAATAGC 
CTTGGCCAGG TGTCCTGCAG CTGGAGCCTG CTGGTGGACC GGCCGAACTT GGCACAGACA 
GCCCCTTCAT TCTCTAGTGT TCTGAAGGAC AGTGTTGTCA TTGAGGGCCA GGATTTTGTG 
CTACGGTGCT CAGTGCAGGG GACTCCAGCA CCCCGGGTCA CGTGGCTGCT CAATGGACAG 
CCCATCCAGT TTGCTCACTC CATCTGTGAG GCAGGCGTGG CTGAGCTCCA CATCCAGGAT 
GCCCTGCCAG AGGACCGTGG CACCTACACC TGCCTGGCTG AGAATGCCAT GGGGCAGGTG 
TCCTGCAGCG CCACTGTCAC CGTCCAAGAG AAGAAAGGTG AAGGAGAGAG GGAACACCGT 
CTGTCCCCAG CTCGCAGCAA GCCCATCGCA CCCATCTTCC TGCAGGGCCT CTCCGACCTC 
AAAGTCATGG ATGGAAGCCA GGTCACTATG ACAGTCCAGG TGTCAGGGAA CCCACCTCCG 
GAAGTCATCT GGCTTCACGA CGGGAATGAG ATTCAGGAGT CCGAGGACTT CCACTTCGAG 
CAGAAGGGTG GCTGGCACAG CCTGTGTATC CAGGAGGTGT TCCCGGAGGA CACGGGCACG 
TACACCTGTG AGGCCTGGAA CAGTGCCGGG GAGGTCCGCA CCCGGGCCGT CCTCACCGTG 
CAAGAGCCTC ATGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCTCGTTC GGTGACAGCC 
ACCCTGGGCC AGAGTGTCCT CATCTCCTGC GCCATAGCTG GAGACCCCTT TCCCACCGTG 
CACTGGCTCC GGGATGGCAG AGCCCTCTCC AAGGACAGTG GCCACTTTGA GCTGCTCCAG 
AATGAGGACG TGTTCACCCT GGTTCTAAAG AACGTGCAGC CCTGGCATGC CGGCCAGTAT 
GAGATTCTGC TCAAGAACCG GGTTGGCGAG TGTAGTTGCC AAGTGTCACT GATGCTTCAC 
AACAGCCCTT CCAGAGCCCC ACCACGGGGG CGGGAGCCTG CCAGCTGCGA GGGCCTCTGT 
GGTGGTGGAG GAGTTGGTGC TCATGGTGAT GGTGACCGTC ATGGGACCCT GAGGCCCTGC 
TGGCCAGCAA GAGGGCAAGG TTGGCCTGAG GAGGAAGACG GAGAAGACGT GAGGGGGCTG 
TTGAAGAGAC GGGTGGAGAC CAGGCTGCAC ACAGAAGAGG CCATCCGCCA GCAGGAGGTG 
GGGCAGCTGG ATTTTCGAGA CCTCCTGGGA AAGAAGGTGA GCACCAAGAC CGTCTCAGAA 
GACGACCTGA AGGACATCCC AGCCGAGCAG ATGGATTTCC GCGCCAACCT TCAGCGGCAA 
GTGAAGCCAA AGACCATATC CGAGGAAGAG AGGAAGGTGC ATAGCCCTCA GCAGGTTGAC 
TTCCGGTCTG TCCTCGCCAA GAAGGGGACC CCTAAGACCC CCGTTCCTGA GAAGGCACCT 
CCAAAAGCTG CCACCCCAGA CTTTCGCTCT GTGCTAGGTG GCAAGAAGAA GTCACCCTCG 
GAGAATGGGG GCAACAGCGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACACCT 
GCAGGCGATG CGCAGGCCAT CGGGGCCCTG AAACCCGTAG GCAATGCCAA GCCTGCTGAG 
ACCCCGAAAC CTATAGGTAA TGCCAAGCCT ACCGAGACCC TGAAGCCTGT GGGCAACACC 
AAGCCTGCCG AGACCCTGAA ACCCATAGCC AACGCACAGC CTTCAGGGTC CCTGAAACCT 
GTAACCAACG CACAACCTGC TGAGCCCCAG AAGCCTGTGG GCAATGCCAA GTCTGCTGAG 
ACCTCGAAAC CAGCTGGGAA AGAAGAAGTC AAGGAGGTAA AGAATGATGT GAACTGTAAG 
AAAGGGCAGG TCGGAGCCAC GGGCAATGAA AAGCGACCCG AGAGCCAAGG CTCGGCCCCA 
GTTTTCAAGG AAAAGCTACA AGATGTCCAT GTGGCAGAGG GGGAGAAGCT GCTACTCCAA 
TGCCAGGTGA TCTCTGATCC CCCCGCCACT GTCACCTGGT CGCTGAATGG GAAGACACTC 
AAGACCACAA AATTCATCGT CCTCGCCCAA GAAGGCTCAC GTTTCTCTGT CTCCATCGAG 
AAGGCACTGC CTGAGGACCG AGGTTTGTAC AAGTGTGTAG CCAAGAACAG CGCTGGCCAG 
GCAGAGTGTT CCTGCCAAGT CACTGTGGAC GATGCTCAGA CCAGTGAGAA CACCAAGGCC 
CCGGAGATGA AATCCCGGAG ACCCAAGAGC TCTCTTCCCC CTGTGCTAGG AACAGAGAGC 
GACGCAACTG TGAAAAAGAA ACCTGCCCCC AAGACACCCA CAAAAGCAGC TATGCCCCCT 
CAAATCATCC AGTTCCCCGA GGACCAGAAG GTGCGCGCAG GAGAACCTGT GGAGCTGTTT 
GGGAAAGTGG CTGGCACCCA GCCCATCACC TGCAAGTGGA TGAAGTTCCG AAAGCAGATC 
CAGGAGAGCG AGCATATCAA GGTGGAGAAT GGCGAGAGCG GCAGCAAGCT CACCATCCTG 
GCCGCGCGGC AGGAGCACTG TGGCTGCTAC ACACTGGTGG TGGAGAACAA GCTGGGCAGC 
AGGCAGGCCC AAGTCAACCT CACGGTCGTG GATAAGCCAG ACCCCCCGGC TGGCACACCT 
TGTGCTTCTG ACATTCGGAG TTCCTCACTG ACCCTGTCCT GGTATGGCTC CTCATATGAT 
GGGGGCAGTG CTGTACAGTC CTACAATGTG GAGATCTGGG ACACAGAGGA CAAGGTGTGG 
AAAGAGCTAG CCACGTGCCG CAGCACCTCT TTTAATGTCC AAGACCTGCT GCCTGACCGC 
GAGTACAAAT TCCGAGTGCG TGCAGTCAAC GTCTATGGAA CCAGCGAGCC AAGCCAAGAG 
TCCGAGCTCA CAGCTGTGGG AGAGAAACCT GAAGAGCCAA AGGATGAAGT GGAAGTGTCC 
GATGATGACG AAAAGGAACC TGAGGTTGAT TACCGGACGG TGACTGTTAA CACTGAACAG 
AAAGTGTCTG ATGTGTATGA CATTGAAGAG CGACTAGGAT CTGGGAAGTT TGGACAAGTC 
TTCCGACTTG TGGAAAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTTTT CAAGGCCTAT 
TCCGCCAAGG AGAAAGACAA CATCCGTCAG GAGATCAGCA TCATGAACTG CCTCCACCAC 
CCCAAGCTGG TCCAGTGTGT GGATGCCTTC GAAGAAAAGG CCAACATTGT CATGGTTCTG 
GAGATCGTGT CCGGGGGTGA GCTGTTTGAG CGTATCATTG ACGAGGACTT CGAGCTGACG 
GAGCGGGAGT GTATCAAGTA CATGAGGCAG ATCTCGGAAG GGGTGGAGTA CATCCACAAG 
CAGGGCATTG TGCACCTGGA CCTCAAGCCC GAGAACATCA TGTGTGTCAA CAAGACAGGC 
ACCAGGATCA AGCTCATTGA CTTCGGCCTG GCACGGAGAC TCGAGAACGC TGGGTCTCTG 
AAGGTCCTCT TTGGGACCCC AGAGTTTGTG GCTCCTGAAG TGATCAACTA TGAGCCCATC 
GGCTACGCCA CAGACATGTG GAGCATCGGG GTCATCTGCT ATATTCTGGT CAGCGGACTT 
TCTCCCTTCA TGGGTGACAA TGATAACGAG ACCTTAGCCA ACGTCACTTC AGCCACCTGG 
GACTTCGATG ACGAGGCATT CGATGAGATC TCCGATGACG CAAAGGATTT CATCAGTAAC 
TTGCTGAAGA AAGATATGAA AAACCGTCTG GACTGCACCC AGTGCCTTCA GCACCCATGG 
CTGATGAAAG ACACCAAGAA CATGGAGGCC AAGAAGCTGT CCAAGGACCG GATGAAGAAA 
TACATGGCAA GAAGGAAGTG GCAGAAAACG GGCAATGCTG TGAGAGCCAT CGGGAGACTG 
TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CTTCAACAGG GTCACCAACC 
AGCCCAATCA ATGCGGAGAA ACTAGAGTCT GAAGACGATG TCTCCCAAGC TTTCCTGGAG 
GCTGTTGCTG AGGAAAAGCC CCATGTGAAA CCTTATTTCT CTAAGACCAT CCGTGATCTG 
GAAGTCGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT 
GAGGTGGTCT GGTTCAAGGA TGACCAATCA ATCAGGGAGT CCCGCCACTT CCAGATAGAC 
TACGACGAAG ACGGCAACTG CTCGCTCATC ATCAGCGACG TCTGTGGGGA TGATGACGCC 
AAGTACACCT GCAAGGCTGT CAACAGCCTC GGAGAAGCCA CCTGCACTGC GGAGCTCATC 
GTGGAGACCA TGGAGGAAGG GGAAGGGGAG GAGGGGGGAG AGGAGGAGGA GGAGGAGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_006521702.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521765.1 Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X1, mRNA.

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CloneID	OMu51811
Clone ID Related Accession (Same CDS sequence)	XM_006521703.3 , XM_006521702.1
Accession Version	XM_006521702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGTT TGCCTCGTCA CACATGTCTA AAACCTCCCA CAGTGTGGAT 
CCCTCGAAGG TCAGCTCCAT GCCTCTCACC GAGGCCCCAG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACAGCC AAGTTCGAAG GGAGGGTCCG GGGCTACCCA 
GAGCCCCAGG TGACATGGCA CAGAAAAGGG CAAGCCATCA CCAATGGTGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTTAGT CTTGTCATCC ACACTGTCCG CGAGGAGGAC 
AAGGGCAAAT ACACCTGTGA AGCTAGCAAT GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGAATTC TATGAAGAAG CGTGATCAGC CTGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCACCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTCTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGTGTGTCAA TGTCAGAGAA GAATGGCATG CAGATCCTGG AGATCCGTGG GGTCACTCGG 
GATGACCTGG GCGTGTACAC CTGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCG 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCGCTAGGT TGGCTGTGAG AGGAACCAAA 
GCCCCCAGTC CAGACATCAG GAAGGAGGTG ACCAATGGAG TCTCCAAAGA CCCAGAGACT 
GTGGCTGAAA GTAAGAACTG TCCAAGTCCC CAGAGGAGTG GCTCCTCAGC CCGGGCCACC 
AACAGTCACC TCAAGTCCCC GCAGGAGCCC AAGCCAAAGC TGTGTGAGGA TGCTCCCAGA 
AAGGTCCCGC AGTCCTCCAT CCTGCAGAAG TCCACCAGCA CCATCACTCT GCAGGCCTTG 
AAAGTCCAGC CAGAAGCGAG AGTACCAGCC ATAGGGTCCT TCTCTCCTGG GGAAGACAGG 
AAGAGTCTGG CTGCCCCTCA GCAAGCCACA CTCCCTACCA GACAGTCTAG CCTGGGAGGT 
TCCGTTGGGA ACAAGTTTGT TACCGGGAAC ATCCCCAGAG AGAGCCAAAG GGAGTCAACA 
TTCCCCAGAT TTGAGAGTCA GCCCCAAAGC CAGGAAGTCA CTGAAGGTCA GACAGTCAAG 
TTCATCTGCG AGGTTTCAGG TATACCAAAG CCTGACGTGG GCTGGTTCCT GGAAGGCATC 
CCCGTGAGGA GACGGGAAGG CATCACTGAG GTTTATGAAG ATGGTGTATC CCATCACCTG 
TGCCTGCTGA GAGCCCGGAC GAGAGACAGT GGGAGGTACA GCTGCACAGC ATCCAATAGC 
CTTGGCCAGG TGTCCTGCAG CTGGAGCCTG CTGGTGGACC GGCCGAACTT GGCACAGACA 
GCCCCTTCAT TCTCTAGTGT TCTGAAGGAC AGTGTTGTCA TTGAGGGCCA GGATTTTGTG 
CTACGGTGCT CAGTGCAGGG GACTCCAGCA CCCCGGGTCA CGTGGCTGCT CAATGGACAG 
CCCATCCAGT TTGCTCACTC CATCTGTGAG GCAGGCGTGG CTGAGCTCCA CATCCAGGAT 
GCCCTGCCAG AGGACCGTGG CACCTACACC TGCCTGGCTG AGAATGCCAT GGGGCAGGTG 
TCCTGCAGCG CCACTGTCAC CGTCCAAGAG AAGAAAGGTG AAGGAGAGAG GGAACACCGT 
CTGTCCCCAG CTCGCAGCAA GCCCATCGCA CCCATCTTCC TGCAGGGCCT CTCCGACCTC 
AAAGTCATGG ATGGAAGCCA GGTCACTATG ACAGTCCAGG TGTCAGGGAA CCCACCTCCG 
GAAGTCATCT GGCTTCACGA CGGGAATGAG ATTCAGGAGT CCGAGGACTT CCACTTCGAG 
CAGAAGGGTG GCTGGCACAG CCTGTGTATC CAGGAGGTGT TCCCGGAGGA CACGGGCACG 
TACACCTGTG AGGCCTGGAA CAGTGCCGGG GAGGTCCGCA CCCGGGCCGT CCTCACCGTG 
CAAGAGCCTC ATGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCTCGTTC GGTGACAGCC 
ACCCTGGGCC AGAGTGTCCT CATCTCCTGC GCCATAGCTG GAGACCCCTT TCCCACCGTG 
CACTGGCTCC GGGATGGCAG AGCCCTCTCC AAGGACAGTG GCCACTTTGA GCTGCTCCAG 
AATGAGGACG TGTTCACCCT GGTTCTAAAG AACGTGCAGC CCTGGCATGC CGGCCAGTAT 
GAGATTCTGC TCAAGAACCG GGTTGGCGAG TGTAGTTGCC AAGTGTCACT GATGCTTCAC 
AACAGCCCTT CCAGAGCCCC ACCACGGGGG CGGGAGCCTG CCAGCTGCGA GGGCCTCTGT 
GGTGGTGGAG GAGTTGGTGC TCATGGTGAT GGTGACCGTC ATGGGACCCT GAGGCCCTGC 
TGGCCAGCAA GAGGGCAAGG TTGGCCTGAG GAGGAAGACG GAGAAGACGT GAGGGGGCTG 
TTGAAGAGAC GGGTGGAGAC CAGGCTGCAC ACAGAAGAGG CCATCCGCCA GCAGGAGGTG 
GGGCAGCTGG ATTTTCGAGA CCTCCTGGGA AAGAAGGTGA GCACCAAGAC CGTCTCAGAA 
GACGACCTGA AGGACATCCC AGCCGAGCAG ATGGATTTCC GCGCCAACCT TCAGCGGCAA 
GTGAAGCCAA AGACCATATC CGAGGAAGAG AGGAAGGTGC ATAGCCCTCA GCAGGTTGAC 
TTCCGGTCTG TCCTCGCCAA GAAGGGGACC CCTAAGACCC CCGTTCCTGA GAAGGCACCT 
CCAAAAGCTG CCACCCCAGA CTTTCGCTCT GTGCTAGGTG GCAAGAAGAA GTCACCCTCG 
GAGAATGGGG GCAACAGCGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACACCT 
GCAGGCGATG CGCAGGCCAT CGGGGCCCTG AAACCCGTAG GCAATGCCAA GCCTGCTGAG 
ACCCCGAAAC CTATAGGTAA TGCCAAGCCT ACCGAGACCC TGAAGCCTGT GGGCAACACC 
AAGCCTGCCG AGACCCTGAA ACCCATAGCC AACGCACAGC CTTCAGGGTC CCTGAAACCT 
GTAACCAACG CACAACCTGC TGAGCCCCAG AAGCCTGTGG GCAATGCCAA GTCTGCTGAG 
ACCTCGAAAC CAGCTGGGAA AGAAGAAGTC AAGGAGGTAA AGAATGATGT GAACTGTAAG 
AAAGGGCAGG TCGGAGCCAC GGGCAATGAA AAGCGACCCG AGAGCCAAGG CTCGGCCCCA 
GTTTTCAAGG AAAAGCTACA AGATGTCCAT GTGGCAGAGG GGGAGAAGCT GCTACTCCAA 
TGCCAGGTGA TCTCTGATCC CCCCGCCACT GTCACCTGGT CGCTGAATGG GAAGACACTC 
AAGACCACAA AATTCATCGT CCTCGCCCAA GAAGGCTCAC GTTTCTCTGT CTCCATCGAG 
AAGGCACTGC CTGAGGACCG AGGTTTGTAC AAGTGTGTAG CCAAGAACAG CGCTGGCCAG 
GCAGAGTGTT CCTGCCAAGT CACTGTGGAC GATGCTCAGA CCAGTGAGAA CACCAAGGCC 
CCGGAGATGA AATCCCGGAG ACCCAAGAGC TCTCTTCCCC CTGTGCTAGG AACAGAGAGC 
GACGCAACTG TGAAAAAGAA ACCTGCCCCC AAGACACCCA CAAAAGCAGC TATGCCCCCT 
CAAATCATCC AGTTCCCCGA GGACCAGAAG GTGCGCGCAG GAGAACCTGT GGAGCTGTTT 
GGGAAAGTGG CTGGCACCCA GCCCATCACC TGCAAGTGGA TGAAGTTCCG AAAGCAGATC 
CAGGAGAGCG AGCATATCAA GGTGGAGAAT GGCGAGAGCG GCAGCAAGCT CACCATCCTG 
GCCGCGCGGC AGGAGCACTG TGGCTGCTAC ACACTGGTGG TGGAGAACAA GCTGGGCAGC 
AGGCAGGCCC AAGTCAACCT CACGGTCGTG GATAAGCCAG ACCCCCCGGC TGGCACACCT 
TGTGCTTCTG ACATTCGGAG TTCCTCACTG ACCCTGTCCT GGTATGGCTC CTCATATGAT 
GGGGGCAGTG CTGTACAGTC CTACAATGTG GAGATCTGGG ACACAGAGGA CAAGGTGTGG 
AAAGAGCTAG CCACGTGCCG CAGCACCTCT TTTAATGTCC AAGACCTGCT GCCTGACCGC 
GAGTACAAAT TCCGAGTGCG TGCAGTCAAC GTCTATGGAA CCAGCGAGCC AAGCCAAGAG 
TCCGAGCTCA CAGCTGTGGG AGAGAAACCT GAAGAGCCAA AGGATGAAGT GGAAGTGTCC 
GATGATGACG AAAAGGAACC TGAGGTTGAT TACCGGACGG TGACTGTTAA CACTGAACAG 
AAAGTGTCTG ATGTGTATGA CATTGAAGAG CGACTAGGAT CTGGGAAGTT TGGACAAGTC 
TTCCGACTTG TGGAAAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTTTT CAAGGCCTAT 
TCCGCCAAGG AGAAAGACAA CATCCGTCAG GAGATCAGCA TCATGAACTG CCTCCACCAC 
CCCAAGCTGG TCCAGTGTGT GGATGCCTTC GAAGAAAAGG CCAACATTGT CATGGTTCTG 
GAGATCGTGT CCGGGGGTGA GCTGTTTGAG CGTATCATTG ACGAGGACTT CGAGCTGACG 
GAGCGGGAGT GTATCAAGTA CATGAGGCAG ATCTCGGAAG GGGTGGAGTA CATCCACAAG 
CAGGGCATTG TGCACCTGGA CCTCAAGCCC GAGAACATCA TGTGTGTCAA CAAGACAGGC 
ACCAGGATCA AGCTCATTGA CTTCGGCCTG GCACGGAGAC TCGAGAACGC TGGGTCTCTG 
AAGGTCCTCT TTGGGACCCC AGAGTTTGTG GCTCCTGAAG TGATCAACTA TGAGCCCATC 
GGCTACGCCA CAGACATGTG GAGCATCGGG GTCATCTGCT ATATTCTGGT CAGCGGACTT 
TCTCCCTTCA TGGGTGACAA TGATAACGAG ACCTTAGCCA ACGTCACTTC AGCCACCTGG 
GACTTCGATG ACGAGGCATT CGATGAGATC TCCGATGACG CAAAGGATTT CATCAGTAAC 
TTGCTGAAGA AAGATATGAA AAACCGTCTG GACTGCACCC AGTGCCTTCA GCACCCATGG 
CTGATGAAAG ACACCAAGAA CATGGAGGCC AAGAAGCTGT CCAAGGACCG GATGAAGAAA 
TACATGGCAA GAAGGAAGTG GCAGAAAACG GGCAATGCTG TGAGAGCCAT CGGGAGACTG 
TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CTTCAACAGG GTCACCAACC 
AGCCCAATCA ATGCGGAGAA ACTAGAGTCT GAAGACGATG TCTCCCAAGC TTTCCTGGAG 
GCTGTTGCTG AGGAAAAGCC CCATGTGAAA CCTTATTTCT CTAAGACCAT CCGTGATCTG 
GAAGTCGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT 
GAGGTGGTCT GGTTCAAGGA TGACCAATCA ATCAGGGAGT CCCGCCACTT CCAGATAGAC 
TACGACGAAG ACGGCAACTG CTCGCTCATC ATCAGCGACG TCTGTGGGGA TGATGACGCC 
AAGTACACCT GCAAGGCTGT CAACAGCCTC GGAGAAGCCA CCTGCACTGC GGAGCTCATC 
GTGGAGACCA TGGAGGAAGG GGAAGGGGAG GAGGGGGGAG AGGAGGAGGA GGAGGAGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521765.1
CDS	176..6001
Translation	MGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRNLCVKEGATAKFEGRVRGYPEPQVTWHRKGQAITNGGRFLLDCGVRGTFSLVIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKRDQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSAELSIPGLDNAARLAVRGTKAPSPDIRKEVTNGVSKDPETVAESKNCPSPQRSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALKVQPEARVPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSVGNKFVTGNIPRESQRESTFPRFESQPQSQEVTEGQTVKFICEVSGIPKPDVGWFLEGIPVRRREGITEVYEDGVSHHLCLLRARTRDSGRYSCTASNSLGQVSCSWSLLVDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQPIQFAHSICEAGVAELHIQDALPEDRGTYTCLAENAMGQVSCSATVTVQEKKGEGEREHRLSPARSKPIAPIFLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVHWLRDGRALSKDSGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDGDRHGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKAPPKAATPDFRSVLGGKKKSPSENGGNSAEVLNVKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTKPAETLKPIANAQPSGSLKPVTNAQPAEPQKPVGNAKSAETSKPAGKEEVKEVKNDVNCKKGQVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVISDPPATVTWSLNGKTLKTTKFIVLAQEGSRFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE

Target ORF information:

RefSeq Version	XM_006521702.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521702.1

ORF Insert Sequence:

ATGGGGGACG TGAAACTGTT TGCCTCGTCA CACATGTCTA AAACCTCCCA CAGTGTGGAT 
CCCTCGAAGG TCAGCTCCAT GCCTCTCACC GAGGCCCCAG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACAGCC AAGTTCGAAG GGAGGGTCCG GGGCTACCCA 
GAGCCCCAGG TGACATGGCA CAGAAAAGGG CAAGCCATCA CCAATGGTGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTTAGT CTTGTCATCC ACACTGTCCG CGAGGAGGAC 
AAGGGCAAAT ACACCTGTGA AGCTAGCAAT GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGAATTC TATGAAGAAG CGTGATCAGC CTGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCACCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTCTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGTGTGTCAA TGTCAGAGAA GAATGGCATG CAGATCCTGG AGATCCGTGG GGTCACTCGG 
GATGACCTGG GCGTGTACAC CTGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCG 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCGCTAGGT TGGCTGTGAG AGGAACCAAA 
GCCCCCAGTC CAGACATCAG GAAGGAGGTG ACCAATGGAG TCTCCAAAGA CCCAGAGACT 
GTGGCTGAAA GTAAGAACTG TCCAAGTCCC CAGAGGAGTG GCTCCTCAGC CCGGGCCACC 
AACAGTCACC TCAAGTCCCC GCAGGAGCCC AAGCCAAAGC TGTGTGAGGA TGCTCCCAGA 
AAGGTCCCGC AGTCCTCCAT CCTGCAGAAG TCCACCAGCA CCATCACTCT GCAGGCCTTG 
AAAGTCCAGC CAGAAGCGAG AGTACCAGCC ATAGGGTCCT TCTCTCCTGG GGAAGACAGG 
AAGAGTCTGG CTGCCCCTCA GCAAGCCACA CTCCCTACCA GACAGTCTAG CCTGGGAGGT 
TCCGTTGGGA ACAAGTTTGT TACCGGGAAC ATCCCCAGAG AGAGCCAAAG GGAGTCAACA 
TTCCCCAGAT TTGAGAGTCA GCCCCAAAGC CAGGAAGTCA CTGAAGGTCA GACAGTCAAG 
TTCATCTGCG AGGTTTCAGG TATACCAAAG CCTGACGTGG GCTGGTTCCT GGAAGGCATC 
CCCGTGAGGA GACGGGAAGG CATCACTGAG GTTTATGAAG ATGGTGTATC CCATCACCTG 
TGCCTGCTGA GAGCCCGGAC GAGAGACAGT GGGAGGTACA GCTGCACAGC ATCCAATAGC 
CTTGGCCAGG TGTCCTGCAG CTGGAGCCTG CTGGTGGACC GGCCGAACTT GGCACAGACA 
GCCCCTTCAT TCTCTAGTGT TCTGAAGGAC AGTGTTGTCA TTGAGGGCCA GGATTTTGTG 
CTACGGTGCT CAGTGCAGGG GACTCCAGCA CCCCGGGTCA CGTGGCTGCT CAATGGACAG 
CCCATCCAGT TTGCTCACTC CATCTGTGAG GCAGGCGTGG CTGAGCTCCA CATCCAGGAT 
GCCCTGCCAG AGGACCGTGG CACCTACACC TGCCTGGCTG AGAATGCCAT GGGGCAGGTG 
TCCTGCAGCG CCACTGTCAC CGTCCAAGAG AAGAAAGGTG AAGGAGAGAG GGAACACCGT 
CTGTCCCCAG CTCGCAGCAA GCCCATCGCA CCCATCTTCC TGCAGGGCCT CTCCGACCTC 
AAAGTCATGG ATGGAAGCCA GGTCACTATG ACAGTCCAGG TGTCAGGGAA CCCACCTCCG 
GAAGTCATCT GGCTTCACGA CGGGAATGAG ATTCAGGAGT CCGAGGACTT CCACTTCGAG 
CAGAAGGGTG GCTGGCACAG CCTGTGTATC CAGGAGGTGT TCCCGGAGGA CACGGGCACG 
TACACCTGTG AGGCCTGGAA CAGTGCCGGG GAGGTCCGCA CCCGGGCCGT CCTCACCGTG 
CAAGAGCCTC ATGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCTCGTTC GGTGACAGCC 
ACCCTGGGCC AGAGTGTCCT CATCTCCTGC GCCATAGCTG GAGACCCCTT TCCCACCGTG 
CACTGGCTCC GGGATGGCAG AGCCCTCTCC AAGGACAGTG GCCACTTTGA GCTGCTCCAG 
AATGAGGACG TGTTCACCCT GGTTCTAAAG AACGTGCAGC CCTGGCATGC CGGCCAGTAT 
GAGATTCTGC TCAAGAACCG GGTTGGCGAG TGTAGTTGCC AAGTGTCACT GATGCTTCAC 
AACAGCCCTT CCAGAGCCCC ACCACGGGGG CGGGAGCCTG CCAGCTGCGA GGGCCTCTGT 
GGTGGTGGAG GAGTTGGTGC TCATGGTGAT GGTGACCGTC ATGGGACCCT GAGGCCCTGC 
TGGCCAGCAA GAGGGCAAGG TTGGCCTGAG GAGGAAGACG GAGAAGACGT GAGGGGGCTG 
TTGAAGAGAC GGGTGGAGAC CAGGCTGCAC ACAGAAGAGG CCATCCGCCA GCAGGAGGTG 
GGGCAGCTGG ATTTTCGAGA CCTCCTGGGA AAGAAGGTGA GCACCAAGAC CGTCTCAGAA 
GACGACCTGA AGGACATCCC AGCCGAGCAG ATGGATTTCC GCGCCAACCT TCAGCGGCAA 
GTGAAGCCAA AGACCATATC CGAGGAAGAG AGGAAGGTGC ATAGCCCTCA GCAGGTTGAC 
TTCCGGTCTG TCCTCGCCAA GAAGGGGACC CCTAAGACCC CCGTTCCTGA GAAGGCACCT 
CCAAAAGCTG CCACCCCAGA CTTTCGCTCT GTGCTAGGTG GCAAGAAGAA GTCACCCTCG 
GAGAATGGGG GCAACAGCGC TGAGGTCTTG AATGTCAAGG CTGGGGAAAG CCCCACACCT 
GCAGGCGATG CGCAGGCCAT CGGGGCCCTG AAACCCGTAG GCAATGCCAA GCCTGCTGAG 
ACCCCGAAAC CTATAGGTAA TGCCAAGCCT ACCGAGACCC TGAAGCCTGT GGGCAACACC 
AAGCCTGCCG AGACCCTGAA ACCCATAGCC AACGCACAGC CTTCAGGGTC CCTGAAACCT 
GTAACCAACG CACAACCTGC TGAGCCCCAG AAGCCTGTGG GCAATGCCAA GTCTGCTGAG 
ACCTCGAAAC CAGCTGGGAA AGAAGAAGTC AAGGAGGTAA AGAATGATGT GAACTGTAAG 
AAAGGGCAGG TCGGAGCCAC GGGCAATGAA AAGCGACCCG AGAGCCAAGG CTCGGCCCCA 
GTTTTCAAGG AAAAGCTACA AGATGTCCAT GTGGCAGAGG GGGAGAAGCT GCTACTCCAA 
TGCCAGGTGA TCTCTGATCC CCCCGCCACT GTCACCTGGT CGCTGAATGG GAAGACACTC 
AAGACCACAA AATTCATCGT CCTCGCCCAA GAAGGCTCAC GTTTCTCTGT CTCCATCGAG 
AAGGCACTGC CTGAGGACCG AGGTTTGTAC AAGTGTGTAG CCAAGAACAG CGCTGGCCAG 
GCAGAGTGTT CCTGCCAAGT CACTGTGGAC GATGCTCAGA CCAGTGAGAA CACCAAGGCC 
CCGGAGATGA AATCCCGGAG ACCCAAGAGC TCTCTTCCCC CTGTGCTAGG AACAGAGAGC 
GACGCAACTG TGAAAAAGAA ACCTGCCCCC AAGACACCCA CAAAAGCAGC TATGCCCCCT 
CAAATCATCC AGTTCCCCGA GGACCAGAAG GTGCGCGCAG GAGAACCTGT GGAGCTGTTT 
GGGAAAGTGG CTGGCACCCA GCCCATCACC TGCAAGTGGA TGAAGTTCCG AAAGCAGATC 
CAGGAGAGCG AGCATATCAA GGTGGAGAAT GGCGAGAGCG GCAGCAAGCT CACCATCCTG 
GCCGCGCGGC AGGAGCACTG TGGCTGCTAC ACACTGGTGG TGGAGAACAA GCTGGGCAGC 
AGGCAGGCCC AAGTCAACCT CACGGTCGTG GATAAGCCAG ACCCCCCGGC TGGCACACCT 
TGTGCTTCTG ACATTCGGAG TTCCTCACTG ACCCTGTCCT GGTATGGCTC CTCATATGAT 
GGGGGCAGTG CTGTACAGTC CTACAATGTG GAGATCTGGG ACACAGAGGA CAAGGTGTGG 
AAAGAGCTAG CCACGTGCCG CAGCACCTCT TTTAATGTCC AAGACCTGCT GCCTGACCGC 
GAGTACAAAT TCCGAGTGCG TGCAGTCAAC GTCTATGGAA CCAGCGAGCC AAGCCAAGAG 
TCCGAGCTCA CAGCTGTGGG AGAGAAACCT GAAGAGCCAA AGGATGAAGT GGAAGTGTCC 
GATGATGACG AAAAGGAACC TGAGGTTGAT TACCGGACGG TGACTGTTAA CACTGAACAG 
AAAGTGTCTG ATGTGTATGA CATTGAAGAG CGACTAGGAT CTGGGAAGTT TGGACAAGTC 
TTCCGACTTG TGGAAAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTTTT CAAGGCCTAT 
TCCGCCAAGG AGAAAGACAA CATCCGTCAG GAGATCAGCA TCATGAACTG CCTCCACCAC 
CCCAAGCTGG TCCAGTGTGT GGATGCCTTC GAAGAAAAGG CCAACATTGT CATGGTTCTG 
GAGATCGTGT CCGGGGGTGA GCTGTTTGAG CGTATCATTG ACGAGGACTT CGAGCTGACG 
GAGCGGGAGT GTATCAAGTA CATGAGGCAG ATCTCGGAAG GGGTGGAGTA CATCCACAAG 
CAGGGCATTG TGCACCTGGA CCTCAAGCCC GAGAACATCA TGTGTGTCAA CAAGACAGGC 
ACCAGGATCA AGCTCATTGA CTTCGGCCTG GCACGGAGAC TCGAGAACGC TGGGTCTCTG 
AAGGTCCTCT TTGGGACCCC AGAGTTTGTG GCTCCTGAAG TGATCAACTA TGAGCCCATC 
GGCTACGCCA CAGACATGTG GAGCATCGGG GTCATCTGCT ATATTCTGGT CAGCGGACTT 
TCTCCCTTCA TGGGTGACAA TGATAACGAG ACCTTAGCCA ACGTCACTTC AGCCACCTGG 
GACTTCGATG ACGAGGCATT CGATGAGATC TCCGATGACG CAAAGGATTT CATCAGTAAC 
TTGCTGAAGA AAGATATGAA AAACCGTCTG GACTGCACCC AGTGCCTTCA GCACCCATGG 
CTGATGAAAG ACACCAAGAA CATGGAGGCC AAGAAGCTGT CCAAGGACCG GATGAAGAAA 
TACATGGCAA GAAGGAAGTG GCAGAAAACG GGCAATGCTG TGAGAGCCAT CGGGAGACTG 
TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CTTCAACAGG GTCACCAACC 
AGCCCAATCA ATGCGGAGAA ACTAGAGTCT GAAGACGATG TCTCCCAAGC TTTCCTGGAG 
GCTGTTGCTG AGGAAAAGCC CCATGTGAAA CCTTATTTCT CTAAGACCAT CCGTGATCTG 
GAAGTCGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT 
GAGGTGGTCT GGTTCAAGGA TGACCAATCA ATCAGGGAGT CCCGCCACTT CCAGATAGAC 
TACGACGAAG ACGGCAACTG CTCGCTCATC ATCAGCGACG TCTGTGGGGA TGATGACGCC 
AAGTACACCT GCAAGGCTGT CAACAGCCTC GGAGAAGCCA CCTGCACTGC GGAGCTCATC 
GTGGAGACCA TGGAGGAAGG GGAAGGGGAG GAGGGGGGAG AGGAGGAGGA GGAGGAGGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk ( XM_017316849.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172338.1 Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X3, mRNA.

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CloneID	OMu79248
Clone ID Related Accession (Same CDS sequence)	XM_017316849.1 , XM_017316849.2
Accession Version	XM_017316849.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase, smooth muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG TGAAACTGTT TGCCTCGTCA CACATGTCTA AAACCTCCCA CAGTGTGGAT 
CCCTCGAAGG TCAGCTCCAT GCCTCTCACC GAGGCCCCAG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACAGCC AAGTTCGAAG GGAGGGTCCG GGGCTACCCA 
GAGCCCCAGG TGACATGGCA CAGAAAAGGG CAAGCCATCA CCAATGGTGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTTAGT CTTGTCATCC ACACTGTCCG CGAGGAGGAC 
AAGGGCAAAT ACACCTGTGA AGCTAGCAAT GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGAATTC TATGAAGAAG CGTGATCAGC CTGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCACCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTCTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGTGTGTCAA TGTCAGAGAA GAATGGCATG CAGATCCTGG AGATCCGTGG GGTCACTCGG 
GATGACCTGG GCGTGTACAC CTGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCG 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCGCTAGGT TGGCTGTGAG AGGAACCAAA 
GCCCCCAGTC CAGACATCAG GAAGGAGGTG ACCAATGGAG TCTCCAAAGA CCCAGAGACT 
GTGGCTGAAA GTAAGAACTG TCCAAGTCCC CAGAGGAGTG GCTCCTCAGC CCGGGCCACC 
AACAGTCACC TCAAGTCCCC GCAGGAGCCC AAGCCAAAGC TGTGTGAGGA TGCTCCCAGA 
AAGGTCCCGC AGTCCTCCAT CCTGCAGAAG TCCACCAGCA CCATCACTCT GCAGGCCTTG 
AAAGTCCAGC CAGAAGCGAG AGTACCAGCC ATAGGGTCCT TCTCTCCTGG GGAAGACAGG 
AAGAGTCTGG CTGCCCCTCA GCAAGCCACA CTCCCTACCA GACAGTCTAG CCTGGGAGGT 
TCCGTTGGGA ACAAGTTTGT TACCGGGAAC ATCCCCAGAG AGAGCCAAAG GGAGTCAACA 
TTCCCCAGAT TTGAGAGTCA GCCCCAAAGC CAGGAAGTCA CTGAAGGTCA GACAGTCAAG 
TTCATCTGCG AGGTTTCAGG TATACCAAAG CCTGACGTGG GCTGGTTCCT GGAAGGCATC 
CCCGTGAGGA GACGGGAAGG CATCACTGAG GTTTATGAAG ATGGTGTATC CCATCACCTG 
TGCCTGCTGA GAGCCCGGAC GAGAGACAGT GGGAGGTACA GCTGCACAGC ATCCAATAGC 
CTTGGCCAGG TGTCCTGCAG CTGGAGCCTG CTGGTGGACC GGCCGAACTT GGCACAGACA 
GCCCCTTCAT TCTCTAGTGT TCTGAAGGAC AGTGTTGTCA TTGAGGGCCA GGATTTTGTG 
CTACGGTGCT CAGTGCAGGG GACTCCAGCA CCCCGGGTCA CGTGGCTGCT CAATGGACAG 
CCCATCCAGT TTGCTCACTC CATCTGTGAG GCAGGCGTGG CTGAGCTCCA CATCCAGGAT 
GCCCTGCCAG AGGACCGTGG CACCTACACC TGCCTGGCTG AGAATGCCAT GGGGCAGGTG 
TCCTGCAGCG CCACTGTCAC CGTCCAAGGG AACCCACCTC CGGAAGTCAT CTGGCTTCAC 
GACGGGAATG AGATTCAGGA GTCCGAGGAC TTCCACTTCG AGCAGAAGGG TGGCTGGCAC 
AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG GACACGGGCA CGTACACCTG TGAGGCCTGG 
AACAGTGCCG GGGAGGTCCG CACCCGGGCC GTCCTCACCG TGCAAGAGCC TCATGATGGC 
ACCCAGCCCT GGTTCATCAG CAAGCCTCGT TCGGTGACAG CCACCCTGGG CCAGAGTGTC 
CTCATCTCCT GCGCCATAGC TGGAGACCCC TTTCCCACCG TGCACTGGCT CCGGGATGGC 
AGAGCCCTCT CCAAGGACAG TGGCCACTTT GAGCTGCTCC AGAATGAGGA CGTGTTCACC 
CTGGTTCTAA AGAACGTGCA GCCCTGGCAT GCCGGCCAGT ATGAGATTCT GCTCAAGAAC 
CGGGTTGGCG AGTGTAGTTG CCAAGTGTCA CTGATGCTTC ACAACAGCCC TTCCAGAGCC 
CCACCACGGG GGCGGGAGCC TGCCAGCTGC GAGGGCCTCT GTGGTGGTGG AGGAGTTGGT 
GCTCATGGTG ATGGTGACCG TCATGGGACC CTGAGGCCCT GCTGGCCAGC AAGAGGGCAA 
GGTTGGCCTG AGGAGGAAGA CGGAGAAGAC GTGAGGGGGC TGTTGAAGAG ACGGGTGGAG 
ACCAGGCTGC ACACAGAAGA GGCCATCCGC CAGCAGGAGG TGGGGCAGCT GGATTTTCGA 
GACCTCCTGG GAAAGAAGGT GAGCACCAAG ACCGTCTCAG AAGACGACCT GAAGGACATC 
CCAGCCGAGC AGATGGATTT CCGCGCCAAC CTTCAGCGGC AAGTGAAGCC AAAGACCATA 
TCCGAGGAAG AGAGGAAGGT GCATAGCCCT CAGCAGGTTG ACTTCCGGTC TGTCCTCGCC 
AAGAAGGGGA CCCCTAAGAC CCCCGTTCCT GAGAAGGCAC CTCCAAAAGC TGCCACCCCA 
GACTTTCGCT CTGTGCTAGG TGGCAAGAAG AAGTCACCCT CGGAGAATGG GGGCAACAGC 
GCTGAGGTCT TGAATGTCAA GGCTGGGGAA AGCCCCACAC CTGCAGGCGA TGCGCAGGCC 
ATCGGGGCCC TGAAACCCGT AGGCAATGCC AAGCCTGCTG AGACCCCGAA ACCTATAGGT 
AATGCCAAGC CTACCGAGAC CCTGAAGCCT GTGGGCAACA CCAAGCCTGC CGAGACCCTG 
AAACCCATAG CCAACGCACA GCCTTCAGGG TCCCTGAAAC CTGTAACCAA CGCACAACCT 
GCTGAGCCCC AGAAGCCTGT GGGCAATGCC AAGTCTGCTG AGACCTCGAA ACCAGCTGGG 
AAAGAAGAAG TCAAGGAGGT AAAGAATGAT GTGAACTGTA AGAAAGGGCA GGTCGGAGCC 
ACGGGCAATG AAAAGCGACC CGAGAGCCAA GGCTCGGCCC CAGTTTTCAA GGAAAAGCTA 
CAAGATGTCC ATGTGGCAGA GGGGGAGAAG CTGCTACTCC AATGCCAGGT GATCTCTGAT 
CCCCCCGCCA CTGTCACCTG GTCGCTGAAT GGGAAGACAC TCAAGACCAC AAAATTCATC 
GTCCTCGCCC AAGAAGGCTC ACGTTTCTCT GTCTCCATCG AGAAGGCACT GCCTGAGGAC 
CGAGGTTTGT ACAAGTGTGT AGCCAAGAAC AGCGCTGGCC AGGCAGAGTG TTCCTGCCAA 
GTCACTGTGG ACGATGCTCA GACCAGTGAG AACACCAAGG CCCCGGAGAT GAAATCCCGG 
AGACCCAAGA GCTCTCTTCC CCCTGTGCTA GGAACAGAGA GCGACGCAAC TGTGAAAAAG 
AAACCTGCCC CCAAGACACC CACAAAAGCA GCTATGCCCC CTCAAATCAT CCAGTTCCCC 
GAGGACCAGA AGGTGCGCGC AGGAGAACCT GTGGAGCTGT TTGGGAAAGT GGCTGGCACC 
CAGCCCATCA CCTGCAAGTG GATGAAGTTC CGAAAGCAGA TCCAGGAGAG CGAGCATATC 
AAGGTGGAGA ATGGCGAGAG CGGCAGCAAG CTCACCATCC TGGCCGCGCG GCAGGAGCAC 
TGTGGCTGCT ACACACTGGT GGTGGAGAAC AAGCTGGGCA GCAGGCAGGC CCAAGTCAAC 
CTCACGGTCG TGGATAAGCC AGACCCCCCG GCTGGCACAC CTTGTGCTTC TGACATTCGG 
AGTTCCTCAC TGACCCTGTC CTGGTATGGC TCCTCATATG ATGGGGGCAG TGCTGTACAG 
TCCTACAATG TGGAGATCTG GGACACAGAG GACAAGGTGT GGAAAGAGCT AGCCACGTGC 
CGCAGCACCT CTTTTAATGT CCAAGACCTG CTGCCTGACC GCGAGTACAA ATTCCGAGTG 
CGTGCAGTCA ACGTCTATGG AACCAGCGAG CCAAGCCAAG AGTCCGAGCT CACAGCTGTG 
GGAGAGAAAC CTGAAGAGCC AAAGGATGAA GTGGAAGTGT CCGATGATGA CGAAAAGGAA 
CCTGAGGTTG ATTACCGGAC GGTGACTGTT AACACTGAAC AGAAAGTGTC TGATGTGTAT 
GACATTGAAG AGCGACTAGG ATCTGGGAAG TTTGGACAAG TCTTCCGACT TGTGGAAAAG 
AAAACTGGAA AAATCTGGGC AGGGAAATTT TTCAAGGCCT ATTCCGCCAA GGAGAAAGAC 
AACATCCGTC AGGAGATCAG CATCATGAAC TGCCTCCACC ACCCCAAGCT GGTCCAGTGT 
GTGGATGCCT TCGAAGAAAA GGCCAACATT GTCATGGTTC TGGAGATCGT GTCCGGGGGT 
GAGCTGTTTG AGCGTATCAT TGACGAGGAC TTCGAGCTGA CGGAGCGGGA GTGTATCAAG 
TACATGAGGC AGATCTCGGA AGGGGTGGAG TACATCCACA AGCAGGGCAT TGTGCACCTG 
GACCTCAAGC CCGAGAACAT CATGTGTGTC AACAAGACAG GCACCAGGAT CAAGCTCATT 
GACTTCGGCC TGGCACGGAG ACTCGAGAAC GCTGGGTCTC TGAAGGTCCT CTTTGGGACC 
CCAGAGTTTG TGGCTCCTGA AGTGATCAAC TATGAGCCCA TCGGCTACGC CACAGACATG 
TGGAGCATCG GGGTCATCTG CTATATTCTG GTCAGCGGAC TTTCTCCCTT CATGGGTGAC 
AATGATAACG AGACCTTAGC CAACGTCACT TCAGCCACCT GGGACTTCGA TGACGAGGCA 
TTCGATGAGA TCTCCGATGA CGCAAAGGAT TTCATCAGTA ACTTGCTGAA GAAAGATATG 
AAAAACCGTC TGGACTGCAC CCAGTGCCTT CAGCACCCAT GGCTGATGAA AGACACCAAG 
AACATGGAGG CCAAGAAGCT GTCCAAGGAC CGGATGAAGA AATACATGGC AAGAAGGAAG 
TGGCAGAAAA CGGGCAATGC TGTGAGAGCC ATCGGGAGAC TGTCCTCTAT GGCAATGATC 
TCAGGGCTCA GTGGCAGGAA ATCTTCAACA GGGTCACCAA CCAGCCCAAT CAATGCGGAG 
AAACTAGAGT CTGAAGACGA TGTCTCCCAA GCTTTCCTGG AGGCTGTTGC TGAGGAAAAG 
CCCCATGTGA AACCTTATTT CTCTAAGACC ATCCGTGATC TGGAAGTCGT GGAGGGAAGC 
GCTGCTAGAT TTGACTGCAA GATTGAAGGA TACCCAGACC CTGAGGTGGT CTGGTTCAAG 
GATGACCAAT CAATCAGGGA GTCCCGCCAC TTCCAGATAG ACTACGACGA AGACGGCAAC 
TGCTCGCTCA TCATCAGCGA CGTCTGTGGG GATGATGACG CCAAGTACAC CTGCAAGGCT 
GTCAACAGCC TCGGAGAAGC CACCTGCACT GCGGAGCTCA TCGTGGAGAC CATGGAGGAA 
GGGGAAGGGG AGGAGGGGGG AGAGGAGGAG GAGGAGGAGG AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172338.1
CDS	176..5863
Translation	MGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRNLCVKEGATAKFEGRVRGYPEPQVTWHRKGQAITNGGRFLLDCGVRGTFSLVIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKRDQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSAELSIPGLDNAARLAVRGTKAPSPDIRKEVTNGVSKDPETVAESKNCPSPQRSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALKVQPEARVPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSVGNKFVTGNIPRESQRESTFPRFESQPQSQEVTEGQTVKFICEVSGIPKPDVGWFLEGIPVRRREGITEVYEDGVSHHLCLLRARTRDSGRYSCTASNSLGQVSCSWSLLVDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQPIQFAHSICEAGVAELHIQDALPEDRGTYTCLAENAMGQVSCSATVTVQGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVHWLRDGRALSKDSGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDGDRHGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKAPPKAATPDFRSVLGGKKKSPSENGGNSAEVLNVKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTKPAETLKPIANAQPSGSLKPVTNAQPAEPQKPVGNAKSAETSKPAGKEEVKEVKNDVNCKKGQVGATGNEKRPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVISDPPATVTWSLNGKTLKTTKFIVLAQEGSRFSVSIEKALPEDRGLYKCVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILAARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEDDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEEE

Target ORF information:

RefSeq Version	XM_017316849.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, light polypeptide kinase (Mylk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316849.1

ORF Insert Sequence:

ATGGGGGACG TGAAACTGTT TGCCTCGTCA CACATGTCTA AAACCTCCCA CAGTGTGGAT 
CCCTCGAAGG TCAGCTCCAT GCCTCTCACC GAGGCCCCAG CTTTCATTCT GCCCCCTCGG 
AACCTCTGCG TCAAAGAAGG AGCCACAGCC AAGTTCGAAG GGAGGGTCCG GGGCTACCCA 
GAGCCCCAGG TGACATGGCA CAGAAAAGGG CAAGCCATCA CCAATGGTGG CCGCTTCCTG 
CTGGACTGTG GAGTGCGGGG GACTTTTAGT CTTGTCATCC ACACTGTCCG CGAGGAGGAC 
AAGGGCAAAT ACACCTGTGA AGCTAGCAAT GGCAGTGGCG CCCGCCAGGT GACCGTGGAG 
CTGACTGTGG AAGGGAATTC TATGAAGAAG CGTGATCAGC CTGTTCTTTC TAAAGCCTCA 
GGATTTCCAG GGGAGACTCG TCCTAGCATC TGGGGTGAGT GCCCACCAAA GTTTGCCACC 
AAGCTGGGCA GAGCTGTGGT CAAAGAAGGG CAGATGGGGC GATTCTCCTG CAAGATCACT 
GGCCGGCCTC CACCACAGGT CACCTGGCTC AAGGGAAATG TCCCGTTGCA GCCAAGTGCC 
CGTGTGTCAA TGTCAGAGAA GAATGGCATG CAGATCCTGG AGATCCGTGG GGTCACTCGG 
GATGACCTGG GCGTGTACAC CTGCATGGTG GTGAATGGGT CAGGGAAAGC CTCCATGTCG 
GCGGAGCTCT CCATCCCAGG TTTGGACAAT GCCGCTAGGT TGGCTGTGAG AGGAACCAAA 
GCCCCCAGTC CAGACATCAG GAAGGAGGTG ACCAATGGAG TCTCCAAAGA CCCAGAGACT 
GTGGCTGAAA GTAAGAACTG TCCAAGTCCC CAGAGGAGTG GCTCCTCAGC CCGGGCCACC 
AACAGTCACC TCAAGTCCCC GCAGGAGCCC AAGCCAAAGC TGTGTGAGGA TGCTCCCAGA 
AAGGTCCCGC AGTCCTCCAT CCTGCAGAAG TCCACCAGCA CCATCACTCT GCAGGCCTTG 
AAAGTCCAGC CAGAAGCGAG AGTACCAGCC ATAGGGTCCT TCTCTCCTGG GGAAGACAGG 
AAGAGTCTGG CTGCCCCTCA GCAAGCCACA CTCCCTACCA GACAGTCTAG CCTGGGAGGT 
TCCGTTGGGA ACAAGTTTGT TACCGGGAAC ATCCCCAGAG AGAGCCAAAG GGAGTCAACA 
TTCCCCAGAT TTGAGAGTCA GCCCCAAAGC CAGGAAGTCA CTGAAGGTCA GACAGTCAAG 
TTCATCTGCG AGGTTTCAGG TATACCAAAG CCTGACGTGG GCTGGTTCCT GGAAGGCATC 
CCCGTGAGGA GACGGGAAGG CATCACTGAG GTTTATGAAG ATGGTGTATC CCATCACCTG 
TGCCTGCTGA GAGCCCGGAC GAGAGACAGT GGGAGGTACA GCTGCACAGC ATCCAATAGC 
CTTGGCCAGG TGTCCTGCAG CTGGAGCCTG CTGGTGGACC GGCCGAACTT GGCACAGACA 
GCCCCTTCAT TCTCTAGTGT TCTGAAGGAC AGTGTTGTCA TTGAGGGCCA GGATTTTGTG 
CTACGGTGCT CAGTGCAGGG GACTCCAGCA CCCCGGGTCA CGTGGCTGCT CAATGGACAG 
CCCATCCAGT TTGCTCACTC CATCTGTGAG GCAGGCGTGG CTGAGCTCCA CATCCAGGAT 
GCCCTGCCAG AGGACCGTGG CACCTACACC TGCCTGGCTG AGAATGCCAT GGGGCAGGTG 
TCCTGCAGCG CCACTGTCAC CGTCCAAGGG AACCCACCTC CGGAAGTCAT CTGGCTTCAC 
GACGGGAATG AGATTCAGGA GTCCGAGGAC TTCCACTTCG AGCAGAAGGG TGGCTGGCAC 
AGCCTGTGTA TCCAGGAGGT GTTCCCGGAG GACACGGGCA CGTACACCTG TGAGGCCTGG 
AACAGTGCCG GGGAGGTCCG CACCCGGGCC GTCCTCACCG TGCAAGAGCC TCATGATGGC 
ACCCAGCCCT GGTTCATCAG CAAGCCTCGT TCGGTGACAG CCACCCTGGG CCAGAGTGTC 
CTCATCTCCT GCGCCATAGC TGGAGACCCC TTTCCCACCG TGCACTGGCT CCGGGATGGC 
AGAGCCCTCT CCAAGGACAG TGGCCACTTT GAGCTGCTCC AGAATGAGGA CGTGTTCACC 
CTGGTTCTAA AGAACGTGCA GCCCTGGCAT GCCGGCCAGT ATGAGATTCT GCTCAAGAAC 
CGGGTTGGCG AGTGTAGTTG CCAAGTGTCA CTGATGCTTC ACAACAGCCC TTCCAGAGCC 
CCACCACGGG GGCGGGAGCC TGCCAGCTGC GAGGGCCTCT GTGGTGGTGG AGGAGTTGGT 
GCTCATGGTG ATGGTGACCG TCATGGGACC CTGAGGCCCT GCTGGCCAGC AAGAGGGCAA 
GGTTGGCCTG AGGAGGAAGA CGGAGAAGAC GTGAGGGGGC TGTTGAAGAG ACGGGTGGAG 
ACCAGGCTGC ACACAGAAGA GGCCATCCGC CAGCAGGAGG TGGGGCAGCT GGATTTTCGA 
GACCTCCTGG GAAAGAAGGT GAGCACCAAG ACCGTCTCAG AAGACGACCT GAAGGACATC 
CCAGCCGAGC AGATGGATTT CCGCGCCAAC CTTCAGCGGC AAGTGAAGCC AAAGACCATA 
TCCGAGGAAG AGAGGAAGGT GCATAGCCCT CAGCAGGTTG ACTTCCGGTC TGTCCTCGCC 
AAGAAGGGGA CCCCTAAGAC CCCCGTTCCT GAGAAGGCAC CTCCAAAAGC TGCCACCCCA 
GACTTTCGCT CTGTGCTAGG TGGCAAGAAG AAGTCACCCT CGGAGAATGG GGGCAACAGC 
GCTGAGGTCT TGAATGTCAA GGCTGGGGAA AGCCCCACAC CTGCAGGCGA TGCGCAGGCC 
ATCGGGGCCC TGAAACCCGT AGGCAATGCC AAGCCTGCTG AGACCCCGAA ACCTATAGGT 
AATGCCAAGC CTACCGAGAC CCTGAAGCCT GTGGGCAACA CCAAGCCTGC CGAGACCCTG 
AAACCCATAG CCAACGCACA GCCTTCAGGG TCCCTGAAAC CTGTAACCAA CGCACAACCT 
GCTGAGCCCC AGAAGCCTGT GGGCAATGCC AAGTCTGCTG AGACCTCGAA ACCAGCTGGG 
AAAGAAGAAG TCAAGGAGGT AAAGAATGAT GTGAACTGTA AGAAAGGGCA GGTCGGAGCC 
ACGGGCAATG AAAAGCGACC CGAGAGCCAA GGCTCGGCCC CAGTTTTCAA GGAAAAGCTA 
CAAGATGTCC ATGTGGCAGA GGGGGAGAAG CTGCTACTCC AATGCCAGGT GATCTCTGAT 
CCCCCCGCCA CTGTCACCTG GTCGCTGAAT GGGAAGACAC TCAAGACCAC AAAATTCATC 
GTCCTCGCCC AAGAAGGCTC ACGTTTCTCT GTCTCCATCG AGAAGGCACT GCCTGAGGAC 
CGAGGTTTGT ACAAGTGTGT AGCCAAGAAC AGCGCTGGCC AGGCAGAGTG TTCCTGCCAA 
GTCACTGTGG ACGATGCTCA GACCAGTGAG AACACCAAGG CCCCGGAGAT GAAATCCCGG 
AGACCCAAGA GCTCTCTTCC CCCTGTGCTA GGAACAGAGA GCGACGCAAC TGTGAAAAAG 
AAACCTGCCC CCAAGACACC CACAAAAGCA GCTATGCCCC CTCAAATCAT CCAGTTCCCC 
GAGGACCAGA AGGTGCGCGC AGGAGAACCT GTGGAGCTGT TTGGGAAAGT GGCTGGCACC 
CAGCCCATCA CCTGCAAGTG GATGAAGTTC CGAAAGCAGA TCCAGGAGAG CGAGCATATC 
AAGGTGGAGA ATGGCGAGAG CGGCAGCAAG CTCACCATCC TGGCCGCGCG GCAGGAGCAC 
TGTGGCTGCT ACACACTGGT GGTGGAGAAC AAGCTGGGCA GCAGGCAGGC CCAAGTCAAC 
CTCACGGTCG TGGATAAGCC AGACCCCCCG GCTGGCACAC CTTGTGCTTC TGACATTCGG 
AGTTCCTCAC TGACCCTGTC CTGGTATGGC TCCTCATATG ATGGGGGCAG TGCTGTACAG 
TCCTACAATG TGGAGATCTG GGACACAGAG GACAAGGTGT GGAAAGAGCT AGCCACGTGC 
CGCAGCACCT CTTTTAATGT CCAAGACCTG CTGCCTGACC GCGAGTACAA ATTCCGAGTG 
CGTGCAGTCA ACGTCTATGG AACCAGCGAG CCAAGCCAAG AGTCCGAGCT CACAGCTGTG 
GGAGAGAAAC CTGAAGAGCC AAAGGATGAA GTGGAAGTGT CCGATGATGA CGAAAAGGAA 
CCTGAGGTTG ATTACCGGAC GGTGACTGTT AACACTGAAC AGAAAGTGTC TGATGTGTAT 
GACATTGAAG AGCGACTAGG ATCTGGGAAG TTTGGACAAG TCTTCCGACT TGTGGAAAAG 
AAAACTGGAA AAATCTGGGC AGGGAAATTT TTCAAGGCCT ATTCCGCCAA GGAGAAAGAC 
AACATCCGTC AGGAGATCAG CATCATGAAC TGCCTCCACC ACCCCAAGCT GGTCCAGTGT 
GTGGATGCCT TCGAAGAAAA GGCCAACATT GTCATGGTTC TGGAGATCGT GTCCGGGGGT 
GAGCTGTTTG AGCGTATCAT TGACGAGGAC TTCGAGCTGA CGGAGCGGGA GTGTATCAAG 
TACATGAGGC AGATCTCGGA AGGGGTGGAG TACATCCACA AGCAGGGCAT TGTGCACCTG 
GACCTCAAGC CCGAGAACAT CATGTGTGTC AACAAGACAG GCACCAGGAT CAAGCTCATT 
GACTTCGGCC TGGCACGGAG ACTCGAGAAC GCTGGGTCTC TGAAGGTCCT CTTTGGGACC 
CCAGAGTTTG TGGCTCCTGA AGTGATCAAC TATGAGCCCA TCGGCTACGC CACAGACATG 
TGGAGCATCG GGGTCATCTG CTATATTCTG GTCAGCGGAC TTTCTCCCTT CATGGGTGAC 
AATGATAACG AGACCTTAGC CAACGTCACT TCAGCCACCT GGGACTTCGA TGACGAGGCA 
TTCGATGAGA TCTCCGATGA CGCAAAGGAT TTCATCAGTA ACTTGCTGAA GAAAGATATG 
AAAAACCGTC TGGACTGCAC CCAGTGCCTT CAGCACCCAT GGCTGATGAA AGACACCAAG 
AACATGGAGG CCAAGAAGCT GTCCAAGGAC CGGATGAAGA AATACATGGC AAGAAGGAAG 
TGGCAGAAAA CGGGCAATGC TGTGAGAGCC ATCGGGAGAC TGTCCTCTAT GGCAATGATC 
TCAGGGCTCA GTGGCAGGAA ATCTTCAACA GGGTCACCAA CCAGCCCAAT CAATGCGGAG 
AAACTAGAGT CTGAAGACGA TGTCTCCCAA GCTTTCCTGG AGGCTGTTGC TGAGGAAAAG 
CCCCATGTGA AACCTTATTT CTCTAAGACC ATCCGTGATC TGGAAGTCGT GGAGGGAAGC 
GCTGCTAGAT TTGACTGCAA GATTGAAGGA TACCCAGACC CTGAGGTGGT CTGGTTCAAG 
GATGACCAAT CAATCAGGGA GTCCCGCCAC TTCCAGATAG ACTACGACGA AGACGGCAAC 
TGCTCGCTCA TCATCAGCGA CGTCTGTGGG GATGATGACG CCAAGTACAC CTGCAAGGCT 
GTCAACAGCC TCGGAGAAGC CACCTGCACT GCGGAGCTCA TCGTGGAGAC CATGGAGGAA 
GGGGAAGGGG AGGAGGGGGG AGAGGAGGAG GAGGAGGAGG AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Mylk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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