MYLK cDNA ORF clone, Homo sapiens(human)

The following MYLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16204 NM_053032.4
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu16204 XM_017006472.2
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu16167 NM_053031.4
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu16167 XM_017006473.1
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu94223 XM_017006470.2
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu94224 XM_017006471.2
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu105369 XM_024453532.1
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu105370 XM_011512860.3
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu105371 XM_011512861.3
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu105372 XM_024453534.1
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu16237 NM_053027.4
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant 3A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu16318 NM_053026.4
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu16325 NM_053028.4
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant 3B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu17800 NM_053025.4
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu59147 NM_001321309.2
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu16318 XM_024453535.1
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu17800 XM_024453533.1
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu17800 XM_024453537.1
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu17800 XM_024453536.1
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu59145 XM_011512860.2
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu59146 XM_011512861.2
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu94222 XM_017006469.2
Latest version!
Homo sapiens myosin light chain kinase (MYLK), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
View Old Accession Versions >>
OHu17800 NM_053025.3 Homo sapiens myosin light chain kinase (MYLK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu16325 NM_053028.3 Homo sapiens myosin light chain kinase (MYLK), transcript variant 3B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu94224 XM_017006471.1 Homo sapiens myosin light chain kinase (MYLK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu16318 NM_053026.3 Homo sapiens myosin light chain kinase (MYLK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu16237 NM_053027.3 Homo sapiens myosin light chain kinase (MYLK), transcript variant 3A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu16204 XM_017006472.1 Homo sapiens myosin light chain kinase (MYLK), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu16167 NM_053031.3 Homo sapiens myosin light chain kinase (MYLK), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu16204 NM_053032.2 Homo sapiens myosin light chain kinase (MYLK), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu16204 NM_053032.3 Homo sapiens myosin light chain kinase (MYLK), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu59147 NM_001321309.1 Homo sapiens myosin light chain kinase (MYLK), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu94222 XM_017006469.1 Homo sapiens myosin light chain kinase (MYLK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu94223 XM_017006470.1 Homo sapiens myosin light chain kinase (MYLK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16204
    Clone ID Related Accession (Same CDS sequence) NM_053032.3 , XM_017006472.1 , XM_017006472.2 , NM_053032.2 , NM_053032.4
    Accession Version NM_053032.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB016380.1, BC064420.1 and BC113456.1. On Jun 16, 2016 this sequence version replaced NM_053032.2. Transcript Variant: This variant (8) encodes the longer isoform of kinase related protein, telokin. It is longer than variant 7 by one codon at the splicing junction between the first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749364.1, SRR1660809.172995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444260.1
    CDS314..778
    Translation

    Target ORF information:

    RefSeq Version NM_053032.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053032.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16204
    Clone ID Related Accession (Same CDS sequence) NM_053032.3 , XM_017006472.1 , XM_017006472.2 , NM_053032.2 , NM_053032.4
    Accession Version NM_053032.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product myosin light chain kinase, smooth muscle isoform 8
    Comment

    1
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    181
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    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444260.1
    Translation

    Target ORF information:

    RefSeq Version NM_053032.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053032.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16204
    Clone ID Related Accession (Same CDS sequence) NM_053032.3 , XM_017006472.1 , XM_017006472.2 , NM_053032.2 , NM_053032.4
    Accession Version NM_053032.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB016380.1, BC064420.1, BC113456.1 and AC023165.20. On Jun 2, 2019 this sequence version replaced NM_053032.3. Transcript Variant: This variant (8) encodes the longer isoform of kinase related protein, telokin. It is longer than variant 7 by one codon at the splicing junction between the first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.155458.1, SRR1660805.112415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444260.1
    CDS255..719
    Translation

    Target ORF information:

    RefSeq Version NM_053032.4
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053032.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16204
    Clone ID Related Accession (Same CDS sequence) NM_053032.3 , XM_017006472.1 , XM_017006472.2 , NM_053032.2 , NM_053032.4
    Accession Version XM_017006472.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861961.1
    CDS173..637
    Translation

    Target ORF information:

    RefSeq Version XM_017006472.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16204
    Clone ID Related Accession (Same CDS sequence) NM_053032.3 , XM_017006472.1 , XM_017006472.2 , NM_053032.2 , NM_053032.4
    Accession Version XM_017006472.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861961.1
    CDS125..589
    Translation

    Target ORF information:

    RefSeq Version XM_017006472.2
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006472.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16167
    Clone ID Related Accession (Same CDS sequence) NM_053031.4 , NM_053031.3 , XM_017006473.1
    Accession Version NM_053031.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB016380.1, BC064420.1, BC113458.1 and AC023165.20. On Jun 2, 2019 this sequence version replaced NM_053031.3. Transcript Variant: This variant (7) encodes the shorter isoform of kinase related protein, telokin. The first exon corresponds to intron 30 and the remainder of the transcript corresponds to the last two exons of the gene. It is shorter than variant 8 by one codon at the splicing junction between the first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.94043.1, SRR1803611.164732.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444259.1
    CDS255..716
    Translation

    Target ORF information:

    RefSeq Version NM_053031.4
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053031.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16167
    Clone ID Related Accession (Same CDS sequence) NM_053031.4 , NM_053031.3 , XM_017006473.1
    Accession Version NM_053031.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB016380.1, BC064420.1 and BC113458.1. On Jun 16, 2016 this sequence version replaced NM_053031.2. Transcript Variant: This variant (7) encodes the shorter isoform of kinase related protein, telokin. The first exon corresponds to intron 30 and the remainder of the transcript corresponds to the last two exons of the gene. It is shorter than variant 8 by one codon at the splicing junction between the first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064420.1, SRR1660809.94043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444259.1
    CDS314..775
    Translation

    Target ORF information:

    RefSeq Version NM_053031.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053031.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16167
    Clone ID Related Accession (Same CDS sequence) NM_053031.4 , NM_053031.3 , XM_017006473.1
    Accession Version XM_017006473.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861962.1
    CDS125..586
    Translation

    Target ORF information:

    RefSeq Version XM_017006473.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG 
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94223
    Clone ID Related Accession (Same CDS sequence) XM_017006470.1 , XM_017006470.2
    Accession Version XM_017006470.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861959.1
    CDS150..2291
    Translation

    Target ORF information:

    RefSeq Version XM_017006470.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94223
    Clone ID Related Accession (Same CDS sequence) XM_017006470.1 , XM_017006470.2
    Accession Version XM_017006470.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861959.1
    CDS150..2291
    Translation

    Target ORF information:

    RefSeq Version XM_017006470.2
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006470.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94224
    Clone ID Related Accession (Same CDS sequence) XM_017006471.2 , XM_017006471.1
    Accession Version XM_017006471.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861960.1
    CDS200..2344
    Translation

    Target ORF information:

    RefSeq Version XM_017006471.2
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006471.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94224
    Clone ID Related Accession (Same CDS sequence) XM_017006471.2 , XM_017006471.1
    Accession Version XM_017006471.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861960.1
    CDS200..2344
    Translation

    Target ORF information:

    RefSeq Version XM_017006471.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006471.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105369
    Clone ID Related Accession (Same CDS sequence) XM_024453532.1
    Accession Version XM_024453532.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCACCTG GCCAGCTGCC CAAGCAGACC ATGGGGGATG TGAAGCTGGT TGCCTCGTCA 
    CACATTTCCA AAACCTCCCT CAGTGTGGAT CCCTCAAGAG TTGACTCCAT GCCCCTGACA
    GAGGCCCCTG CTTTCATTTT GCCCCCTCGG AACCTCTGCA TCAAAGAAGG AGCCACCGCC
    AAGTTCGAAG GGCGGGTCCG GGGTTACCCA GAGCCCCAGG TGACATGGCA CAGAAACGGG
    CAACCCATCA CCAGCGGGGG CCGCTTCCTG CTGGATTGCG GCATCCGGGG GACTTTCAGC
    CTTGTGATTC ATGCTGTCCA TGAGGAGGAC AGGGGAAAGT ATACCTGTGA AGCCACCAAT
    GGCAGTGGTG CTCGCCAGGT GACAGTGGAG TTGACAGTAG AAGGAAGTTT TGCGAAGCAG
    CTTGGTCAGC CTGTTGTTTC CAAAACCTTA GGGGATAGAT TTTCAGCTCC AGCAGTGGAG
    ACCCGTCCTA GCATCTGGGG GGAGTGCCCA CCAAAGTTTG CTACCAAGCT GGGCCGAGTT
    GTGGTCAAAG AAGGACAGAT GGGACGATTC TCCTGCAAGA TCACTGGCCG GCCCCAACCG
    CAGGTCACCT GGCTCAAGGG AAATGTTCCA CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT
    GAGAAGAACG GCATGCAGGT TCTGGAAATC CATGGAGTCA ACCAAGATGA CGTGGGAGTG
    TACACGTGCC TGGTGGTGAA CGGGTCGGGG AAGGCCTCGA TGTCAGCTGA ACTTTCCATC
    CAAGGTTTGG ACAGTGCCAA TAGGTCATTT GTGAGAGAAA CAAAAGCCAC CAATTCAGAT
    GTCAGGAAAG AGGTGACCAA TGTAATCTCA AAGGAGTCGA AGCTGGACAG TCTGGAGGCT
    GCAGCCAAAA GCAAGAACTG CTCCAGCCCC CAGAGAGGTG GCTCCCCACC CTGGGCTGCA
    AACAGCCAGC CTCAGCCCCC AAGGGAGTCC AAGCTGGAGT CATGCAAGGA CTCGCCCAGA
    ACGGCCCCGC AGACCCCGGT CCTTCAGAAG ACTTCCAGCT CCATCACCCT GCAGGCCGCA
    AGAGTTCAGC CGGAACCAAG AGCACCAGGC CTGGGGGTCC TATCACCTTC TGGAGAAGAG
    AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG
    AGCCAAGATG TTGTGAGCAA GGCTGCTAAC AGGAGAATCC CCATGGAGGG CCAGAGGGAT
    TCAGCATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAAGGA AAATCAAACT
    GTCAAGTTCA GATGTGAAGT TTCCGGGATT CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA
    GGCACCCCCG TGAGGAGACA GGAAGGCAGC ATTGAGGTTT ATGAAGATGC TGGCTCCCAT
    TACCTCTGCC TGCTGAAAGC CCGGACCAGG GACAGTGGGA CATACAGCTG CACTGCTTCC
    AACGCCCAAG GCCAGCTGTC CTGTAGCTGG ACCCTCCAAG TGGAAAGGCT TGCCGTGATG
    GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG AAGGACTGCG CTGTTATTGA GGGCCAGGAT
    TTTGTGCTGC AGTGCTCCGT ACGGGGGACC CCAGTGCCCC GGATCACTTG GCTGCTGAAT
    GGGCAGCCCA TCCAGTACGC TCGCTCCACC TGCGAGGCCG GCGTGGCTGA GCTCCACATC
    CAGGATGCCC TGCCGGAGGA CCATGGCACC TACACCTGCC TAGCTGAGAA TGCCTTGGGG
    CAGGTGTCCT GCAGCGCCTG GGTCACCGTC CATGAAAAGA AGAGTAGCAG GAAGAGTGAG
    TACCTTCTGC CTGTGGCTCC CAGCAAGCCC ACTGCACCCA TCTTCCTGCA GGGCCTCTCT
    GATCTCAAAG TCATGGATGG AAGCCAGGTC ACTATGACTG TCCAAGTGTC AGGGAATCCA
    CCCCCTGAAG TCATCTGGCT GCACAATGGG AATGAGATCC AAGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGAACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC GGAGGACACG
    GGCACGTACA CCTGCGAGGC CTGGAACAGC GCTGGAGAGG TCCGCACCCA GGCCGTGCTC
    ACGGTACAAG AGCCTCACGA TGGCACCCAG CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG
    ACAGCCTCCC TGGGCCAGAG TGTCCTCATC TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT
    ACCGTGCACT GGCTCAGAGA TGGCAAAGCC CTCTGCAAAG ACACTGGCCA CTTCGAGGTG
    CTTCAGAATG AGGACGTGTT CACCCTGGTT CTAAAGAAGG TGCAGCCCTG GCATGCCGGC
    CAGTATGAGA TCCTGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAACA GCTCTGCCAG AGCCCTTCCA CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC
    CTCTGTGGTG GAGGAGTTGG TGCTGATGGT GGTGGTAGTG ACCGCTATGG GTCCCTGAGG
    CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG CTAGAGGAGG AAGACGGCGA GGACGTGCGA
    GGGGTGCTGA AGAGGCGCGT GGAGACGAGG CAGCACACTG AGGAGGCGAT CCGCCAGCAG
    GAGGTGGAGC AGCTGGACTT CCGAGACCTC CTGGGGAAGA AGGTGAGTAC AAAGACCCTA
    TCGGAAGACG ACCTGAAGGA GATCCCAGCC GAGCAGATGG ATTTCCGTGC CAACCTGCAG
    CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG GAAGAGAGGA AGGTGCACAG CCCCCAGCAG
    GTCGATTTTC GCTCTGTCCT GGCCAAGAAG GGGACTTCCA AGACCCCCGT GCCTGAGAAG
    GTGCCACCGC CAAAACCTGC CACCCCGGAT TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA
    TTACCAGCAG AGAATGGCAG CAGCAGTGCC GAGACCCTGA ATGCCAAGGC AGTGGAGAGT
    TCCAAGCCCC TGAGCAATGC ACAGCCTTCA GGGCCCTTGA AACCCGTGGG CAACGCCAAG
    CCTGCTGAGA CCCTGAAGCC AATGGGCAAC GCCAAGCCTG CCGAGACCCT GAAGCCCATG
    GGCAATGCCA AGCCTGATGA GAACCTGAAA TCCGCTAGCA AAGAAGAACT CAAGAAAGAC
    GTTAAGAATG ATGTGAACTG CAAGAGAGGC CATGCAGGGA CCACAGATAA TGAAAAGAGA
    TCAGAGAGCC AGGGGACAGC CCCAGCCTTC AAGCAGAAGC TGCAAGATGT TCATGTGGCA
    GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG GTGTCTTCTG ACCCCCCAGC CACCATCATC
    TGGACGCTGA ACGGAAAGAC CCTCAAGACC ACCAAGTTCA TCATCCTCTC CCAGGAAGGC
    TCACTCTGCT CCGTCTCCAT CGAGAAGGCA CTGCCTGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ATGACGCTGG CCAGGCGGAG TGCTCCTGCC AAGTCACCGT GGATGATGCT
    CCAGCCAGTG AGAACACCAA GGCCCCAGAG ATGAAATCCC GGAGGCCCAA GAGCTCTCTT
    CCTCCCGTGC TAGGAACTGA GAGTGATGCG ACTGTGAAAA AGAAACCTGC CCCCAAGACA
    CCTCCGAAGG CAGCAATGCC CCCTCAGATC ATCCAGTTCC CTGAGGACCA GAAGGTACGC
    GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA GTGACAGGCA CTCAGCCCAT CACCTGTACC
    TGGATGAAGT TCCGAAAGCA GATCCAGGAA AGCGAGCACA TGAAGGTGGA GAACAGCGAG
    AATGGCAGCA AGCTCACCAT CCTGGCCGCG CGCCAGGAGC ACTGCGGCTG CTACACACTG
    CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG GCCCAGGTCA ACCTCACTGT CGTGGATAAG
    CCAGACCCCC CAGCTGGCAC ACCTTGTGCC TCTGACATTC GGAGCTCCTC ACTGACCCTG
    TCCTGGTATG GCTCCTCATA TGATGGGGGC AGTGCTGTAC AGTCCTACAG CATCGAGATC
    TGGGACTCAG CCAACAAGAC GTGGAAGGAA CTAGCCACAT GCCGCAGCAC CTCTTTCAAC
    GTCCAGGACC TGCTGCCTGA CCACGAATAT AAGTTCCGTG TACGTGCAAT CAACGTGTAT
    GGAACCAGTG AGCCAAGCCA GGAGTCTGAA CTCACAACGG TAGGAGAGAA ACCTGAAGAG
    CCGAAGGATG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCCGAGGT TGATTACCGG
    ACAGTGACAA TCAATACTGA ACAAAAAGTA TCTGACTTCT ACGACATTGA GGAGAGATTA
    GGATCTGGGA AATTTGGACA GGTCTTTCGA CTTGTAGAAA AGAAAACTCG AAAAGTCTGG
    GCAGGGAAGT TCTTCAAGGC ATATTCAGCA AAAGAGAAAG AGAATATCCG GCAGGAGATT
    AGCATCATGA ACTGCCTCCA CCACCCTAAG CTGGTCCAGT GTGTGGATGC CTTTGAAGAA
    AAGGCCAACA TCGTCATGGT CCTGGAGATC GTGTCAGGAG GGGAGCTGTT TGAGCGCATC
    ATTGACGAGG ACTTTGAGCT GACGGAGCGT GAGTGCATCA AGTACATGCG GCAGATCTCG
    GAGGGAGTGG AGTACATCCA CAAGCAGGGC ATCGTGCACC TGGACCTCAA GCCGGAGAAC
    ATCATGTGTG TCAACAAGAC GGGCACCAGG ATCAAGCTCA TCGACTTTGG TCTGGCCAGG
    AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC CTCTTTGGCA CCCCAGAATT TGTGGCTCCT
    GAAGTGATCA ACTATGAGCC CATCGGCTAC GCCACAGACA TGTGGAGCAT CGGGGTCATC
    TGCTACATCC TAGTCAGTGG CCTTTCCCCC TTCATGGGAG ACAACGATAA CGAAACCTTG
    GCCAACGTTA CCTCAGCCAC CTGGGACTTC GACGACGAGG CATTCGATGA GATCTCCGAC
    GATGCCAAGG ATTTCATCAG CAATCTGCTG AAGAAAGATA TGAAAAACCG CCTGGACTGC
    ACGCAGTGCC TTCAGCATCC ATGGCTAATG AAAGATACCA AGAACATGGA GGCCAAGAAA
    CTCTCCAAGG ACCGGATGAA GAAGTACATG GCAAGAAGGA AATGGCAGAA AACGGGCAAT
    GCTGTGAGAG CCATTGGAAG ACTGTCCTCT ATGGCAATGA TCTCAGGGCT CAGTGGCAGG
    AAATCCTCAA CAGGGTCACC AACCAGCCCG CTCAATGCAG AAAAACTAGA ATCTGAAGAA
    GATGTGTCCC AAGCTTTCCT TGAGGCTGTT GCTGAGGAAA AGCCTCATGT AAAACCCTAT
    TTCTCTAAGA CCATTCGCGA TTTAGAAGTT GTGGAGGGAA GTGCTGCTAG ATTTGACTGC
    AAGATTGAAG GATACCCAGA CCCCGAGGTT GTCTGGTTCA AAGATGACCA GTCAATCAGG
    GAGTCCCGCC ACTTCCAGAT AGACTACGAT GAGGACGGGA ACTGCTCTTT AATTATTAGT
    GATGTTTGCG GGGATGACGA TGCCAAGTAC ACCTGCAAGG CTGTCAACAG TCTTGGAGAA
    GCCACCTGCA CAGCAGAGCT CATTGTGGAA ACGATGGAGG AAGGTGAAGG GGAAGGGGAA
    GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309300.1
    CDS29..5803
    Translation

    Target ORF information:

    RefSeq Version XM_024453532.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCACCTG GCCAGCTGCC CAAGCAGACC ATGGGGGATG TGAAGCTGGT TGCCTCGTCA 
    CACATTTCCA AAACCTCCCT CAGTGTGGAT CCCTCAAGAG TTGACTCCAT GCCCCTGACA
    GAGGCCCCTG CTTTCATTTT GCCCCCTCGG AACCTCTGCA TCAAAGAAGG AGCCACCGCC
    AAGTTCGAAG GGCGGGTCCG GGGTTACCCA GAGCCCCAGG TGACATGGCA CAGAAACGGG
    CAACCCATCA CCAGCGGGGG CCGCTTCCTG CTGGATTGCG GCATCCGGGG GACTTTCAGC
    CTTGTGATTC ATGCTGTCCA TGAGGAGGAC AGGGGAAAGT ATACCTGTGA AGCCACCAAT
    GGCAGTGGTG CTCGCCAGGT GACAGTGGAG TTGACAGTAG AAGGAAGTTT TGCGAAGCAG
    CTTGGTCAGC CTGTTGTTTC CAAAACCTTA GGGGATAGAT TTTCAGCTCC AGCAGTGGAG
    ACCCGTCCTA GCATCTGGGG GGAGTGCCCA CCAAAGTTTG CTACCAAGCT GGGCCGAGTT
    GTGGTCAAAG AAGGACAGAT GGGACGATTC TCCTGCAAGA TCACTGGCCG GCCCCAACCG
    CAGGTCACCT GGCTCAAGGG AAATGTTCCA CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT
    GAGAAGAACG GCATGCAGGT TCTGGAAATC CATGGAGTCA ACCAAGATGA CGTGGGAGTG
    TACACGTGCC TGGTGGTGAA CGGGTCGGGG AAGGCCTCGA TGTCAGCTGA ACTTTCCATC
    CAAGGTTTGG ACAGTGCCAA TAGGTCATTT GTGAGAGAAA CAAAAGCCAC CAATTCAGAT
    GTCAGGAAAG AGGTGACCAA TGTAATCTCA AAGGAGTCGA AGCTGGACAG TCTGGAGGCT
    GCAGCCAAAA GCAAGAACTG CTCCAGCCCC CAGAGAGGTG GCTCCCCACC CTGGGCTGCA
    AACAGCCAGC CTCAGCCCCC AAGGGAGTCC AAGCTGGAGT CATGCAAGGA CTCGCCCAGA
    ACGGCCCCGC AGACCCCGGT CCTTCAGAAG ACTTCCAGCT CCATCACCCT GCAGGCCGCA
    AGAGTTCAGC CGGAACCAAG AGCACCAGGC CTGGGGGTCC TATCACCTTC TGGAGAAGAG
    AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG
    AGCCAAGATG TTGTGAGCAA GGCTGCTAAC AGGAGAATCC CCATGGAGGG CCAGAGGGAT
    TCAGCATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAAGGA AAATCAAACT
    GTCAAGTTCA GATGTGAAGT TTCCGGGATT CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA
    GGCACCCCCG TGAGGAGACA GGAAGGCAGC ATTGAGGTTT ATGAAGATGC TGGCTCCCAT
    TACCTCTGCC TGCTGAAAGC CCGGACCAGG GACAGTGGGA CATACAGCTG CACTGCTTCC
    AACGCCCAAG GCCAGCTGTC CTGTAGCTGG ACCCTCCAAG TGGAAAGGCT TGCCGTGATG
    GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG AAGGACTGCG CTGTTATTGA GGGCCAGGAT
    TTTGTGCTGC AGTGCTCCGT ACGGGGGACC CCAGTGCCCC GGATCACTTG GCTGCTGAAT
    GGGCAGCCCA TCCAGTACGC TCGCTCCACC TGCGAGGCCG GCGTGGCTGA GCTCCACATC
    CAGGATGCCC TGCCGGAGGA CCATGGCACC TACACCTGCC TAGCTGAGAA TGCCTTGGGG
    CAGGTGTCCT GCAGCGCCTG GGTCACCGTC CATGAAAAGA AGAGTAGCAG GAAGAGTGAG
    TACCTTCTGC CTGTGGCTCC CAGCAAGCCC ACTGCACCCA TCTTCCTGCA GGGCCTCTCT
    GATCTCAAAG TCATGGATGG AAGCCAGGTC ACTATGACTG TCCAAGTGTC AGGGAATCCA
    CCCCCTGAAG TCATCTGGCT GCACAATGGG AATGAGATCC AAGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGAACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC GGAGGACACG
    GGCACGTACA CCTGCGAGGC CTGGAACAGC GCTGGAGAGG TCCGCACCCA GGCCGTGCTC
    ACGGTACAAG AGCCTCACGA TGGCACCCAG CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG
    ACAGCCTCCC TGGGCCAGAG TGTCCTCATC TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT
    ACCGTGCACT GGCTCAGAGA TGGCAAAGCC CTCTGCAAAG ACACTGGCCA CTTCGAGGTG
    CTTCAGAATG AGGACGTGTT CACCCTGGTT CTAAAGAAGG TGCAGCCCTG GCATGCCGGC
    CAGTATGAGA TCCTGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAACA GCTCTGCCAG AGCCCTTCCA CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC
    CTCTGTGGTG GAGGAGTTGG TGCTGATGGT GGTGGTAGTG ACCGCTATGG GTCCCTGAGG
    CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG CTAGAGGAGG AAGACGGCGA GGACGTGCGA
    GGGGTGCTGA AGAGGCGCGT GGAGACGAGG CAGCACACTG AGGAGGCGAT CCGCCAGCAG
    GAGGTGGAGC AGCTGGACTT CCGAGACCTC CTGGGGAAGA AGGTGAGTAC AAAGACCCTA
    TCGGAAGACG ACCTGAAGGA GATCCCAGCC GAGCAGATGG ATTTCCGTGC CAACCTGCAG
    CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG GAAGAGAGGA AGGTGCACAG CCCCCAGCAG
    GTCGATTTTC GCTCTGTCCT GGCCAAGAAG GGGACTTCCA AGACCCCCGT GCCTGAGAAG
    GTGCCACCGC CAAAACCTGC CACCCCGGAT TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA
    TTACCAGCAG AGAATGGCAG CAGCAGTGCC GAGACCCTGA ATGCCAAGGC AGTGGAGAGT
    TCCAAGCCCC TGAGCAATGC ACAGCCTTCA GGGCCCTTGA AACCCGTGGG CAACGCCAAG
    CCTGCTGAGA CCCTGAAGCC AATGGGCAAC GCCAAGCCTG CCGAGACCCT GAAGCCCATG
    GGCAATGCCA AGCCTGATGA GAACCTGAAA TCCGCTAGCA AAGAAGAACT CAAGAAAGAC
    GTTAAGAATG ATGTGAACTG CAAGAGAGGC CATGCAGGGA CCACAGATAA TGAAAAGAGA
    TCAGAGAGCC AGGGGACAGC CCCAGCCTTC AAGCAGAAGC TGCAAGATGT TCATGTGGCA
    GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG GTGTCTTCTG ACCCCCCAGC CACCATCATC
    TGGACGCTGA ACGGAAAGAC CCTCAAGACC ACCAAGTTCA TCATCCTCTC CCAGGAAGGC
    TCACTCTGCT CCGTCTCCAT CGAGAAGGCA CTGCCTGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ATGACGCTGG CCAGGCGGAG TGCTCCTGCC AAGTCACCGT GGATGATGCT
    CCAGCCAGTG AGAACACCAA GGCCCCAGAG ATGAAATCCC GGAGGCCCAA GAGCTCTCTT
    CCTCCCGTGC TAGGAACTGA GAGTGATGCG ACTGTGAAAA AGAAACCTGC CCCCAAGACA
    CCTCCGAAGG CAGCAATGCC CCCTCAGATC ATCCAGTTCC CTGAGGACCA GAAGGTACGC
    GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA GTGACAGGCA CTCAGCCCAT CACCTGTACC
    TGGATGAAGT TCCGAAAGCA GATCCAGGAA AGCGAGCACA TGAAGGTGGA GAACAGCGAG
    AATGGCAGCA AGCTCACCAT CCTGGCCGCG CGCCAGGAGC ACTGCGGCTG CTACACACTG
    CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG GCCCAGGTCA ACCTCACTGT CGTGGATAAG
    CCAGACCCCC CAGCTGGCAC ACCTTGTGCC TCTGACATTC GGAGCTCCTC ACTGACCCTG
    TCCTGGTATG GCTCCTCATA TGATGGGGGC AGTGCTGTAC AGTCCTACAG CATCGAGATC
    TGGGACTCAG CCAACAAGAC GTGGAAGGAA CTAGCCACAT GCCGCAGCAC CTCTTTCAAC
    GTCCAGGACC TGCTGCCTGA CCACGAATAT AAGTTCCGTG TACGTGCAAT CAACGTGTAT
    GGAACCAGTG AGCCAAGCCA GGAGTCTGAA CTCACAACGG TAGGAGAGAA ACCTGAAGAG
    CCGAAGGATG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCCGAGGT TGATTACCGG
    ACAGTGACAA TCAATACTGA ACAAAAAGTA TCTGACTTCT ACGACATTGA GGAGAGATTA
    GGATCTGGGA AATTTGGACA GGTCTTTCGA CTTGTAGAAA AGAAAACTCG AAAAGTCTGG
    GCAGGGAAGT TCTTCAAGGC ATATTCAGCA AAAGAGAAAG AGAATATCCG GCAGGAGATT
    AGCATCATGA ACTGCCTCCA CCACCCTAAG CTGGTCCAGT GTGTGGATGC CTTTGAAGAA
    AAGGCCAACA TCGTCATGGT CCTGGAGATC GTGTCAGGAG GGGAGCTGTT TGAGCGCATC
    ATTGACGAGG ACTTTGAGCT GACGGAGCGT GAGTGCATCA AGTACATGCG GCAGATCTCG
    GAGGGAGTGG AGTACATCCA CAAGCAGGGC ATCGTGCACC TGGACCTCAA GCCGGAGAAC
    ATCATGTGTG TCAACAAGAC GGGCACCAGG ATCAAGCTCA TCGACTTTGG TCTGGCCAGG
    AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC CTCTTTGGCA CCCCAGAATT TGTGGCTCCT
    GAAGTGATCA ACTATGAGCC CATCGGCTAC GCCACAGACA TGTGGAGCAT CGGGGTCATC
    TGCTACATCC TAGTCAGTGG CCTTTCCCCC TTCATGGGAG ACAACGATAA CGAAACCTTG
    GCCAACGTTA CCTCAGCCAC CTGGGACTTC GACGACGAGG CATTCGATGA GATCTCCGAC
    GATGCCAAGG ATTTCATCAG CAATCTGCTG AAGAAAGATA TGAAAAACCG CCTGGACTGC
    ACGCAGTGCC TTCAGCATCC ATGGCTAATG AAAGATACCA AGAACATGGA GGCCAAGAAA
    CTCTCCAAGG ACCGGATGAA GAAGTACATG GCAAGAAGGA AATGGCAGAA AACGGGCAAT
    GCTGTGAGAG CCATTGGAAG ACTGTCCTCT ATGGCAATGA TCTCAGGGCT CAGTGGCAGG
    AAATCCTCAA CAGGGTCACC AACCAGCCCG CTCAATGCAG AAAAACTAGA ATCTGAAGAA
    GATGTGTCCC AAGCTTTCCT TGAGGCTGTT GCTGAGGAAA AGCCTCATGT AAAACCCTAT
    TTCTCTAAGA CCATTCGCGA TTTAGAAGTT GTGGAGGGAA GTGCTGCTAG ATTTGACTGC
    AAGATTGAAG GATACCCAGA CCCCGAGGTT GTCTGGTTCA AAGATGACCA GTCAATCAGG
    GAGTCCCGCC ACTTCCAGAT AGACTACGAT GAGGACGGGA ACTGCTCTTT AATTATTAGT
    GATGTTTGCG GGGATGACGA TGCCAAGTAC ACCTGCAAGG CTGTCAACAG TCTTGGAGAA
    GCCACCTGCA CAGCAGAGCT CATTGTGGAA ACGATGGAGG AAGGTGAAGG GGAAGGGGAA
    GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105370
    Clone ID Related Accession (Same CDS sequence) XM_011512860.3
    Accession Version XM_011512860.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCACCTG GCCAGCTGCC CAAGCAGACC ATGGGGGATG TGAAGCTGGT TGCCTCGTCA 
    CACATTTCCA AAACCTCCCT CAGTGTGGAT CCCTCAAGAG TTGACTCCAT GCCCCTGACA
    GAGGCCCCTG CTTTCATTTT GCCCCCTCGG AACCTCTGCA TCAAAGAAGG AGCCACCGCC
    AAGTTCGAAG GGCGGGTCCG GGGTTACCCA GAGCCCCAGG TGACATGGCA CAGAAACGGG
    CAACCCATCA CCAGCGGGGG CCGCTTCCTG CTGGATTGCG GCATCCGGGG GACTTTCAGC
    CTTGTGATTC ATGCTGTCCA TGAGGAGGAC AGGGGAAAGT ATACCTGTGA AGCCACCAAT
    GGCAGTGGTG CTCGCCAGGT GACAGTGGAG TTGACAGTAG AAGGAAGTTT TGCGAAGCAG
    CTTGGTCAGC CTGTTGTTTC CAAAACCTTA GGGGATAGAT TTTCAGCTCC AGCAGTGGAG
    ACCCGTCCTA GCATCTGGGG GGAGTGCCCA CCAAAGTTTG CTACCAAGCT GGGCCGAGTT
    GTGGTCAAAG AAGGACAGAT GGGACGATTC TCCTGCAAGA TCACTGGCCG GCCCCAACCG
    CAGGTCACCT GGCTCAAGGG AAATGTTCCA CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT
    GAGAAGAACG GCATGCAGGT TCTGGAAATC CATGGAGTCA ACCAAGATGA CGTGGGAGTG
    TACACGTGCC TGGTGGTGAA CGGGTCGGGG AAGGCCTCGA TGTCAGCTGA ACTTTCCATC
    CAAGGTTTGG ACAGTGCCAA TAGGTCATTT GTGAGAGAAA CAAAAGCCAC CAATTCAGAT
    GTCAGGAAAG AGGTGACCAA TGTAATCTCA AAGGAGTCGA AGCTGGACAG TCTGGAGGCT
    GCAGCCAAAA GCAAGAACTG CTCCAGCCCC CAGAGAGGTG GCTCCCCACC CTGGGCTGCA
    AACAGCCAGC CTCAGCCCCC AAGGGAGTCC AAGCTGGAGT CATGCAAGGA CTCGCCCAGA
    ACGGCCCCGC AGACCCCGGT CCTTCAGAAG ACTTCCAGCT CCATCACCCT GCAGGCCGCA
    AGAGTTCAGC CGGAACCAAG AGCACCAGGC CTGGGGGTCC TATCACCTTC TGGAGAAGAG
    AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG
    AGCCAAGATG TTGTGAGCAA GGCTGCTAAC AGGAGAATCC CCATGGAGGG CCAGAGGGAT
    TCAGCATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAAGGA AAATCAAACT
    GTCAAGTTCA GATGTGAAGT TTCCGGGATT CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA
    GGCACCCCCG TGAGGAGACA GGAAGGCAGC ATTGAGGTTT ATGAAGATGC TGGCTCCCAT
    TACCTCTGCC TGCTGAAAGC CCGGACCAGG GACAGTGGGA CATACAGCTG CACTGCTTCC
    AACGCCCAAG GCCAGCTGTC CTGTAGCTGG ACCCTCCAAG TGGAAAGGCT TGCCGTGATG
    GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG AAGGACTGCG CTGTTATTGA GGGCCAGGAT
    TTTGTGCTGC AGTGCTCCGT ACGGGGGACC CCAGTGCCCC GGATCACTTG GCTGCTGAAT
    GGGCAGCCCA TCCAGTACGC TCGCTCCACC TGCGAGGCCG GCGTGGCTGA GCTCCACATC
    CAGGATGCCC TGCCGGAGGA CCATGGCACC TACACCTGCC TAGCTGAGAA TGCCTTGGGG
    CAGGTGTCCT GCAGCGCCTG GGTCACCGTC CATGAAAAGA AGAGTAGCAG GAAGAGTGAG
    TACCTTCTGC CTGTGGCTCC CAGCAAGCCC ACTGCACCCA TCTTCCTGCA GGGCCTCTCT
    GATCTCAAAG TCATGGATGG AAGCCAGGTC ACTATGACTG TCCAAGTGTC AGGGAATCCA
    CCCCCTGAAG TCATCTGGCT GCACAATGGG AATGAGATCC AAGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGAACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC GGAGGACACG
    GGCACGTACA CCTGCGAGGC CTGGAACAGC GCTGGAGAGG TCCGCACCCA GGCCGTGCTC
    ACGGTACAAG AGCCTCACGA TGGCACCCAG CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG
    ACAGCCTCCC TGGGCCAGAG TGTCCTCATC TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT
    ACCGTGCACT GGCTCAGAGA TGGCAAAGCC CTCTGCAAAG ACACTGGCCA CTTCGAGGTG
    CTTCAGAATG AGGACGTGTT CACCCTGGTT CTAAAGAAGG TGCAGCCCTG GCATGCCGGC
    CAGTATGAGA TCCTGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAACA GCTCTGCCAG AGCCCTTCCA CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC
    CTCTGTGGTG GAGGAGTTGG TGCTGATGGT GGTGGTAGTG ACCGCTATGG GTCCCTGAGG
    CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG CTAGAGGAGG AAGACGGCGA GGACGTGCGA
    GGGGTGCTGA AGAGGCGCGT GGAGACGAGG CAGCACACTG AGGAGGCGAT CCGCCAGCAG
    GAGGTGGAGC AGCTGGACTT CCGAGACCTC CTGGGGAAGA AGGTGAGTAC AAAGACCCTA
    TCGGAAGACG ACCTGAAGGA GATCCCAGCC GAGCAGATGG ATTTCCGTGC CAACCTGCAG
    CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG GAAGAGAGGA AGGTGCACAG CCCCCAGCAG
    GTCGATTTTC GCTCTGTCCT GGCCAAGAAG GGGACTTCCA AGACCCCCGT GCCTGAGAAG
    GTGCCACCGC CAAAACCTGC CACCCCGGAT TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA
    TTACCAGCAG AGAATGGCAG CAGCAGTGCC GAGACCCTGA ATGCCAAGGC AGTGGAGAGT
    TCCAAGCCCC TGAGCAATGC ACAGCCTTCA GGGCCCTTGA AACCCGTGGG CAACGCCAAG
    CCTGCTGAGA CCCTGAAGCC AATGGGCAAC GCCAAGCCTG CCGAGACCCT GAAGCCCATG
    GGCAATGCCA AGCCTGATGA GAACCTGAAA TCCGCTAGCA AAGAAGAACT CAAGAAAGAC
    GTTAAGAATG ATGTGAACTG CAAGAGAGGC CATGCAGGGA CCACAGATAA TGAAAAGAGA
    TCAGAGAGCC AGGGGACAGC CCCAGCCTTC AAGCAGAAGC TGCAAGATGT TCATGTGGCA
    GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG GTGTCTTCTG ACCCCCCAGC CACCATCATC
    TGGACGCTGA ACGGAAAGAC CCTCAAGACC ACCAAGTTCA TCATCCTCTC CCAGGAAGGC
    TCACTCTGCT CCGTCTCCAT CGAGAAGGCA CTGCCTGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ATGACGCTGG CCAGGCGGAG TGCTCCTGCC AAGTCACCGT GGATGATGCT
    CCAGCCAGTG AGAACACCAA GGCCCCAGAG ATGAAATCCC GGAGGCCCAA GAGCTCTCTT
    CCTCCCGTGC TAGGAACTGA GAGTGATGCG ACTGTGAAAA AGAAACCTGC CCCCAAGACA
    CCTCCGAAGG CAGCAATGCC CCCTCAGATC ATCCAGTTCC CTGAGGACCA GAAGGTACGC
    GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA GTGACAGGCA CTCAGCCCAT CACCTGTACC
    TGGATGAAGT TCCGAAAGCA GATCCAGGAA AGCGAGCACA TGAAGGTGGA GAACAGCGAG
    AATGGCAGCA AGCTCACCAT CCTGGCCGCG CGCCAGGAGC ACTGCGGCTG CTACACACTG
    CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG GCCCAGGTCA ACCTCACTGT CGTGGATAAG
    CCAGACCCCC CAGCTGGCAC ACCTTGTGCC TCTGACATTC GGAGCTCCTC ACTGACCCTG
    TCCTGGTATG GCTCCTCATA TGATGGGGGC AGTGCTGTAC AGTCCTACAG CATCGAGATC
    TGGGACTCAG CCAACAAGAC GTGGAAGGAA CTAGCCACAT GCCGCAGCAC CTCTTTCAAC
    GTCCAGGACC TGCTGCCTGA CCACGAATAT AAGTTCCGTG TACGTGCAAT CAACGTGTAT
    GGAACCAGTG AGCCAAGCCA GGAGTCTGAA CTCACAACGG TAGGAGAGAA ACCTGAAGAG
    CCGAAGGATG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCCGAGGT TGATTACCGG
    ACAGTGACAA TCAATACTGA ACAAAAAGTA TCTGACTTCT ACGACATTGA GGAGAGATTA
    GGATCTGGGA AATTTGGACA GGTCTTTCGA CTTGTAGAAA AGAAAACTCG AAAAGTCTGG
    GCAGGGAAGT TCTTCAAGGC ATATTCAGCA AAAGAGAAAG AGAATATCCG GCAGGAGATT
    AGCATCATGA ACTGCCTCCA CCACCCTAAG CTGGTCCAGT GTGTGGATGC CTTTGAAGAA
    AAGGCCAACA TCGTCATGGT CCTGGAGATC GTGTCAGGAG GGGAGCTGTT TGAGCGCATC
    ATTGACGAGG ACTTTGAGCT GACGGAGCGT GAGTGCATCA AGTACATGCG GCAGATCTCG
    GAGGGAGTGG AGTACATCCA CAAGCAGGGC ATCGTGCACC TGGACCTCAA GCCGGAGAAC
    ATCATGTGTG TCAACAAGAC GGGCACCAGG ATCAAGCTCA TCGACTTTGG TCTGGCCAGG
    AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC CTCTTTGGCA CCCCAGAATT TGTGGCTCCT
    GAAGTGATCA ACTATGAGCC CATCGGCTAC GCCACAGACA TGTGGAGCAT CGGGGTCATC
    TGCTACATCC TAGTCAGTGG CCTTTCCCCC TTCATGGGAG ACAACGATAA CGAAACCTTG
    GCCAACGTTA CCTCAGCCAC CTGGGACTTC GACGACGAGG CATTCGATGA GATCTCCGAC
    GATGCCAAGG ATTTCATCAG CAATCTGCTG AAGAAAGATA TGAAAAACCG CCTGGACTGC
    ACGCAGTGCC TTCAGCATCC ATGGCTAATG AAAGATACCA AGAACATGGA GGCCAAGAAA
    CTCTCCAAGG ACCGGATGAA GAAGTACATG GCAAGAAGGA AATGGCAGAA AACGGGCAAT
    GCTGTGAGAG CCATTGGAAG ACTGTCCTCT ATGGCAATGA TCTCAGGGCT CAGTGGCAGG
    AAATCCTCAA CAGGGTCACC AACCAGCCCG CTCAATGCAG AAAAACTAGA ATCTGAAGAT
    GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT GAGGAAAAGC CTCATGTAAA ACCCTATTTC
    TCTAAGACCA TTCGCGATTT AGAAGTTGTG GAGGGAAGTG CTGCTAGATT TGACTGCAAG
    ATTGAAGGAT ACCCAGACCC CGAGGTTGTC TGGTTCAAAG ATGACCAGTC AATCAGGGAG
    TCCCGCCACT TCCAGATAGA CTACGATGAG GACGGGAACT GCTCTTTAAT TATTAGTGAT
    GTTTGCGGGG ATGACGATGC CAAGTACACC TGCAAGGCTG TCAACAGTCT TGGAGAAGCC
    ACCTGCACAG CAGAGCTCAT TGTGGAAACG ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG
    GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511162.2
    CDS34..5805
    Translation

    Target ORF information:

    RefSeq Version XM_011512860.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011512860.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCACCTG GCCAGCTGCC CAAGCAGACC ATGGGGGATG TGAAGCTGGT TGCCTCGTCA 
    CACATTTCCA AAACCTCCCT CAGTGTGGAT CCCTCAAGAG TTGACTCCAT GCCCCTGACA
    GAGGCCCCTG CTTTCATTTT GCCCCCTCGG AACCTCTGCA TCAAAGAAGG AGCCACCGCC
    AAGTTCGAAG GGCGGGTCCG GGGTTACCCA GAGCCCCAGG TGACATGGCA CAGAAACGGG
    CAACCCATCA CCAGCGGGGG CCGCTTCCTG CTGGATTGCG GCATCCGGGG GACTTTCAGC
    CTTGTGATTC ATGCTGTCCA TGAGGAGGAC AGGGGAAAGT ATACCTGTGA AGCCACCAAT
    GGCAGTGGTG CTCGCCAGGT GACAGTGGAG TTGACAGTAG AAGGAAGTTT TGCGAAGCAG
    CTTGGTCAGC CTGTTGTTTC CAAAACCTTA GGGGATAGAT TTTCAGCTCC AGCAGTGGAG
    ACCCGTCCTA GCATCTGGGG GGAGTGCCCA CCAAAGTTTG CTACCAAGCT GGGCCGAGTT
    GTGGTCAAAG AAGGACAGAT GGGACGATTC TCCTGCAAGA TCACTGGCCG GCCCCAACCG
    CAGGTCACCT GGCTCAAGGG AAATGTTCCA CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT
    GAGAAGAACG GCATGCAGGT TCTGGAAATC CATGGAGTCA ACCAAGATGA CGTGGGAGTG
    TACACGTGCC TGGTGGTGAA CGGGTCGGGG AAGGCCTCGA TGTCAGCTGA ACTTTCCATC
    CAAGGTTTGG ACAGTGCCAA TAGGTCATTT GTGAGAGAAA CAAAAGCCAC CAATTCAGAT
    GTCAGGAAAG AGGTGACCAA TGTAATCTCA AAGGAGTCGA AGCTGGACAG TCTGGAGGCT
    GCAGCCAAAA GCAAGAACTG CTCCAGCCCC CAGAGAGGTG GCTCCCCACC CTGGGCTGCA
    AACAGCCAGC CTCAGCCCCC AAGGGAGTCC AAGCTGGAGT CATGCAAGGA CTCGCCCAGA
    ACGGCCCCGC AGACCCCGGT CCTTCAGAAG ACTTCCAGCT CCATCACCCT GCAGGCCGCA
    AGAGTTCAGC CGGAACCAAG AGCACCAGGC CTGGGGGTCC TATCACCTTC TGGAGAAGAG
    AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG
    AGCCAAGATG TTGTGAGCAA GGCTGCTAAC AGGAGAATCC CCATGGAGGG CCAGAGGGAT
    TCAGCATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAAGGA AAATCAAACT
    GTCAAGTTCA GATGTGAAGT TTCCGGGATT CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA
    GGCACCCCCG TGAGGAGACA GGAAGGCAGC ATTGAGGTTT ATGAAGATGC TGGCTCCCAT
    TACCTCTGCC TGCTGAAAGC CCGGACCAGG GACAGTGGGA CATACAGCTG CACTGCTTCC
    AACGCCCAAG GCCAGCTGTC CTGTAGCTGG ACCCTCCAAG TGGAAAGGCT TGCCGTGATG
    GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG AAGGACTGCG CTGTTATTGA GGGCCAGGAT
    TTTGTGCTGC AGTGCTCCGT ACGGGGGACC CCAGTGCCCC GGATCACTTG GCTGCTGAAT
    GGGCAGCCCA TCCAGTACGC TCGCTCCACC TGCGAGGCCG GCGTGGCTGA GCTCCACATC
    CAGGATGCCC TGCCGGAGGA CCATGGCACC TACACCTGCC TAGCTGAGAA TGCCTTGGGG
    CAGGTGTCCT GCAGCGCCTG GGTCACCGTC CATGAAAAGA AGAGTAGCAG GAAGAGTGAG
    TACCTTCTGC CTGTGGCTCC CAGCAAGCCC ACTGCACCCA TCTTCCTGCA GGGCCTCTCT
    GATCTCAAAG TCATGGATGG AAGCCAGGTC ACTATGACTG TCCAAGTGTC AGGGAATCCA
    CCCCCTGAAG TCATCTGGCT GCACAATGGG AATGAGATCC AAGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGAACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC GGAGGACACG
    GGCACGTACA CCTGCGAGGC CTGGAACAGC GCTGGAGAGG TCCGCACCCA GGCCGTGCTC
    ACGGTACAAG AGCCTCACGA TGGCACCCAG CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG
    ACAGCCTCCC TGGGCCAGAG TGTCCTCATC TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT
    ACCGTGCACT GGCTCAGAGA TGGCAAAGCC CTCTGCAAAG ACACTGGCCA CTTCGAGGTG
    CTTCAGAATG AGGACGTGTT CACCCTGGTT CTAAAGAAGG TGCAGCCCTG GCATGCCGGC
    CAGTATGAGA TCCTGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAACA GCTCTGCCAG AGCCCTTCCA CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC
    CTCTGTGGTG GAGGAGTTGG TGCTGATGGT GGTGGTAGTG ACCGCTATGG GTCCCTGAGG
    CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG CTAGAGGAGG AAGACGGCGA GGACGTGCGA
    GGGGTGCTGA AGAGGCGCGT GGAGACGAGG CAGCACACTG AGGAGGCGAT CCGCCAGCAG
    GAGGTGGAGC AGCTGGACTT CCGAGACCTC CTGGGGAAGA AGGTGAGTAC AAAGACCCTA
    TCGGAAGACG ACCTGAAGGA GATCCCAGCC GAGCAGATGG ATTTCCGTGC CAACCTGCAG
    CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG GAAGAGAGGA AGGTGCACAG CCCCCAGCAG
    GTCGATTTTC GCTCTGTCCT GGCCAAGAAG GGGACTTCCA AGACCCCCGT GCCTGAGAAG
    GTGCCACCGC CAAAACCTGC CACCCCGGAT TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA
    TTACCAGCAG AGAATGGCAG CAGCAGTGCC GAGACCCTGA ATGCCAAGGC AGTGGAGAGT
    TCCAAGCCCC TGAGCAATGC ACAGCCTTCA GGGCCCTTGA AACCCGTGGG CAACGCCAAG
    CCTGCTGAGA CCCTGAAGCC AATGGGCAAC GCCAAGCCTG CCGAGACCCT GAAGCCCATG
    GGCAATGCCA AGCCTGATGA GAACCTGAAA TCCGCTAGCA AAGAAGAACT CAAGAAAGAC
    GTTAAGAATG ATGTGAACTG CAAGAGAGGC CATGCAGGGA CCACAGATAA TGAAAAGAGA
    TCAGAGAGCC AGGGGACAGC CCCAGCCTTC AAGCAGAAGC TGCAAGATGT TCATGTGGCA
    GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG GTGTCTTCTG ACCCCCCAGC CACCATCATC
    TGGACGCTGA ACGGAAAGAC CCTCAAGACC ACCAAGTTCA TCATCCTCTC CCAGGAAGGC
    TCACTCTGCT CCGTCTCCAT CGAGAAGGCA CTGCCTGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ATGACGCTGG CCAGGCGGAG TGCTCCTGCC AAGTCACCGT GGATGATGCT
    CCAGCCAGTG AGAACACCAA GGCCCCAGAG ATGAAATCCC GGAGGCCCAA GAGCTCTCTT
    CCTCCCGTGC TAGGAACTGA GAGTGATGCG ACTGTGAAAA AGAAACCTGC CCCCAAGACA
    CCTCCGAAGG CAGCAATGCC CCCTCAGATC ATCCAGTTCC CTGAGGACCA GAAGGTACGC
    GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA GTGACAGGCA CTCAGCCCAT CACCTGTACC
    TGGATGAAGT TCCGAAAGCA GATCCAGGAA AGCGAGCACA TGAAGGTGGA GAACAGCGAG
    AATGGCAGCA AGCTCACCAT CCTGGCCGCG CGCCAGGAGC ACTGCGGCTG CTACACACTG
    CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG GCCCAGGTCA ACCTCACTGT CGTGGATAAG
    CCAGACCCCC CAGCTGGCAC ACCTTGTGCC TCTGACATTC GGAGCTCCTC ACTGACCCTG
    TCCTGGTATG GCTCCTCATA TGATGGGGGC AGTGCTGTAC AGTCCTACAG CATCGAGATC
    TGGGACTCAG CCAACAAGAC GTGGAAGGAA CTAGCCACAT GCCGCAGCAC CTCTTTCAAC
    GTCCAGGACC TGCTGCCTGA CCACGAATAT AAGTTCCGTG TACGTGCAAT CAACGTGTAT
    GGAACCAGTG AGCCAAGCCA GGAGTCTGAA CTCACAACGG TAGGAGAGAA ACCTGAAGAG
    CCGAAGGATG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCCGAGGT TGATTACCGG
    ACAGTGACAA TCAATACTGA ACAAAAAGTA TCTGACTTCT ACGACATTGA GGAGAGATTA
    GGATCTGGGA AATTTGGACA GGTCTTTCGA CTTGTAGAAA AGAAAACTCG AAAAGTCTGG
    GCAGGGAAGT TCTTCAAGGC ATATTCAGCA AAAGAGAAAG AGAATATCCG GCAGGAGATT
    AGCATCATGA ACTGCCTCCA CCACCCTAAG CTGGTCCAGT GTGTGGATGC CTTTGAAGAA
    AAGGCCAACA TCGTCATGGT CCTGGAGATC GTGTCAGGAG GGGAGCTGTT TGAGCGCATC
    ATTGACGAGG ACTTTGAGCT GACGGAGCGT GAGTGCATCA AGTACATGCG GCAGATCTCG
    GAGGGAGTGG AGTACATCCA CAAGCAGGGC ATCGTGCACC TGGACCTCAA GCCGGAGAAC
    ATCATGTGTG TCAACAAGAC GGGCACCAGG ATCAAGCTCA TCGACTTTGG TCTGGCCAGG
    AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC CTCTTTGGCA CCCCAGAATT TGTGGCTCCT
    GAAGTGATCA ACTATGAGCC CATCGGCTAC GCCACAGACA TGTGGAGCAT CGGGGTCATC
    TGCTACATCC TAGTCAGTGG CCTTTCCCCC TTCATGGGAG ACAACGATAA CGAAACCTTG
    GCCAACGTTA CCTCAGCCAC CTGGGACTTC GACGACGAGG CATTCGATGA GATCTCCGAC
    GATGCCAAGG ATTTCATCAG CAATCTGCTG AAGAAAGATA TGAAAAACCG CCTGGACTGC
    ACGCAGTGCC TTCAGCATCC ATGGCTAATG AAAGATACCA AGAACATGGA GGCCAAGAAA
    CTCTCCAAGG ACCGGATGAA GAAGTACATG GCAAGAAGGA AATGGCAGAA AACGGGCAAT
    GCTGTGAGAG CCATTGGAAG ACTGTCCTCT ATGGCAATGA TCTCAGGGCT CAGTGGCAGG
    AAATCCTCAA CAGGGTCACC AACCAGCCCG CTCAATGCAG AAAAACTAGA ATCTGAAGAT
    GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT GAGGAAAAGC CTCATGTAAA ACCCTATTTC
    TCTAAGACCA TTCGCGATTT AGAAGTTGTG GAGGGAAGTG CTGCTAGATT TGACTGCAAG
    ATTGAAGGAT ACCCAGACCC CGAGGTTGTC TGGTTCAAAG ATGACCAGTC AATCAGGGAG
    TCCCGCCACT TCCAGATAGA CTACGATGAG GACGGGAACT GCTCTTTAAT TATTAGTGAT
    GTTTGCGGGG ATGACGATGC CAAGTACACC TGCAAGGCTG TCAACAGTCT TGGAGAAGCC
    ACCTGCACAG CAGAGCTCAT TGTGGAAACG ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG
    GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105371
    Clone ID Related Accession (Same CDS sequence) XM_011512861.3
    Accession Version XM_011512861.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512861.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCACCTG GCCAGCTGCC CAAGCAGACC ATGGGGGATG TGAAGCTGGT TGCCTCGTCA 
    CACATTTCCA AAACCTCCCT CAGTGTGGAT CCCTCAAGAG TTGACTCCAT GCCCCTGACA
    GAGGCCCCTG CTTTCATTTT GCCCCCTCGG AACCTCTGCA TCAAAGAAGG AGCCACCGCC
    AAGTTCGAAG GGCGGGTCCG GGGTTACCCA GAGCCCCAGG TGACATGGCA CAGAAACGGG
    CAACCCATCA CCAGCGGGGG CCGCTTCCTG CTGGATTGCG GCATCCGGGG GACTTTCAGC
    CTTGTGATTC ATGCTGTCCA TGAGGAGGAC AGGGGAAAGT ATACCTGTGA AGCCACCAAT
    GGCAGTGGTG CTCGCCAGGT GACAGTGGAG TTGACAGTAG AAGGAAGTTT TGCGAAGCAG
    CTTGGTCAGC CTGTTGTTTC CAAAACCTTA GGGGATAGAT TTTCAGCTCC AGCAGTGGAG
    ACCCGTCCTA GCATCTGGGG GGAGTGCCCA CCAAAGTTTG CTACCAAGCT GGGCCGAGTT
    GTGGTCAAAG AAGGACAGAT GGGACGATTC TCCTGCAAGA TCACTGGCCG GCCCCAACCG
    CAGGTCACCT GGCTCAAGGG AAATGTTCCA CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT
    GAGAAGAACG GCATGCAGGT TCTGGAAATC CATGGAGTCA ACCAAGATGA CGTGGGAGTG
    TACACGTGCC TGGTGGTGAA CGGGTCGGGG AAGGCCTCGA TGTCAGCTGA ACTTTCCATC
    CAAGGTTTGG ACAGTGCCAA TAGGTCATTT GTGAGAGAAA CAAAAGCCAC CAATTCAGAT
    GTCAGGAAAG AGGTGACCAA TGTAATCTCA AAGGAGTCGA AGCTGGACAG TCTGGAGGCT
    GCAGCCAAAA GCAAGAACTG CTCCAGCCCC CAGAGAGGTG GCTCCCCACC CTGGGCTGCA
    AACAGCCAGC CTCAGCCCCC AAGGGAGTCC AAGCTGGAGT CATGCAAGGA CTCGCCCAGA
    ACGGCCCCGC AGACCCCGGT CCTTCAGAAG ACTTCCAGCT CCATCACCCT GCAGGCCGCA
    AGAGTTCAGC CGGAACCAAG AGCACCAGGC CTGGGGGTCC TATCACCTTC TGGAGAAGAG
    AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG
    AGCCAAGATG TTGTGAGCAA GGCTGCTAAC AGGAGAATCC CCATGGAGGG CCAGAGGGAT
    TCAGCATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAAGGA AAATCAAACT
    GTCAAGTTCA GATGTGAAGT TTCCGGGATT CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA
    GGCACCCCCG TGAGGAGACA GGAAGGCAGC ATTGAGGTTT ATGAAGATGC TGGCTCCCAT
    TACCTCTGCC TGCTGAAAGC CCGGACCAGG GACAGTGGGA CATACAGCTG CACTGCTTCC
    AACGCCCAAG GCCAGCTGTC CTGTAGCTGG ACCCTCCAAG TGGAAAGGCT TGCCGTGATG
    GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG AAGGACTGCG CTGTTATTGA GGGCCAGGAT
    TTTGTGCTGC AGTGCTCCGT ACGGGGGACC CCAGTGCCCC GGATCACTTG GCTGCTGAAT
    GGGCAGCCCA TCCAGTACGC TCGCTCCACC TGCGAGGCCG GCGTGGCTGA GCTCCACATC
    CAGGATGCCC TGCCGGAGGA CCATGGCACC TACACCTGCC TAGCTGAGAA TGCCTTGGGG
    CAGGTGTCCT GCAGCGCCTG GGTCACCGTC CATGAAAAGA AGAGTAGCAG GAAGAGTGAG
    TACCTTCTGC CTGTGGCTCC CAGCAAGCCC ACTGCACCCA TCTTCCTGCA GGGCCTCTCT
    GATCTCAAAG TCATGGATGG AAGCCAGGTC ACTATGACTG TCCAAGTGTC AGGGAATCCA
    CCCCCTGAAG TCATCTGGCT GCACAATGGG AATGAGATCC AAGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGAACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC GGAGGACACG
    GGCACGTACA CCTGCGAGGC CTGGAACAGC GCTGGAGAGG TCCGCACCCA GGCCGTGCTC
    ACGGTACAAG AGCCTCACGA TGGCACCCAG CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG
    ACAGCCTCCC TGGGCCAGAG TGTCCTCATC TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT
    ACCGTGCACT GGCTCAGAGA TGGCAAAGCC CTCTGCAAAG ACACTGGCCA CTTCGAGGTG
    CTTCAGAATG AGGACGTGTT CACCCTGGTT CTAAAGAAGG TGCAGCCCTG GCATGCCGGC
    CAGTATGAGA TCCTGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAACA GCTCTGCCAG AGCCCTTCCA CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC
    CTCTGTGGTG GAGGAGTTGG TGCTGATGGT GGTGGTAGTG ACCGCTATGG GTCCCTGAGG
    CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG CTAGAGGAGG AAGACGGCGA GGACGTGCGA
    GGGGTGCTGA AGAGGCGCGT GGAGACGAGG CAGCACACTG AGGAGGCGAT CCGCCAGCAG
    GAGGTGGAGC AGCTGGACTT CCGAGACCTC CTGGGGAAGA AGGTGAGTAC AAAGACCCTA
    TCGGAAGACG ACCTGAAGGA GATCCCAGCC GAGCAGATGG ATTTCCGTGC CAACCTGCAG
    CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG GAAGAGAGGA AGGTGCACAG CCCCCAGCAG
    GTCGATTTTC GCTCTGTCCT GGCCAAGAAG GGGACTTCCA AGACCCCCGT GCCTGAGAAG
    GTGCCACCGC CAAAACCTGC CACCCCGGAT TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA
    TTACCAGCAG AGAATGGCAG CAGCAGTGCC GAGACCCTGA ATGCCAAGGC AGTGGAGAGT
    TCCAAGCCCC TGAGCAATGC ACAGCCTTCA GGGCCCTTGA AACCCGTGGG CAACGCCAAG
    CCTGCTGAGA CCCTGAAGCC AATGGGCAAC GCCAAGCCTG CCGAGACCCT GAAGCCCATG
    GGCAATGCCA AGCCTGATGA GAACCTGAAA TCCGCTAGCA AAGAAGAACT CAAGAAAGAC
    GTTAAGAATG ATGTGAACTG CAAGAGAGGC CATGCAGGGA CCACAGATAA TGAAAAGAGA
    TCAGAGAGCC AGGGGACAGC CCCAGCCTTC AAGCAGAAGC TGCAAGATGT TCATGTGGCA
    GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG GTGTCTTCTG ACCCCCCAGC CACCATCATC
    TGGACGCTGA ACGGAAAGAC CCTCAAGACC ACCAAGTTCA TCATCCTCTC CCAGGAAGGC
    TCACTCTGCT CCGTCTCCAT CGAGAAGGCA CTGCCTGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ATGACGCTGG CCAGGCGGAG TGCTCCTGCC AAGTCACCGT GGATGATGCT
    CCAGCCAGTG AGAACACCAA GGCCCCAGAG ATGAAATCCC GGAGGCCCAA GAGCTCTCTT
    CCTCCCGTGC TAGGAACTGA GAGTGATGCG ACTGTGAAAA AGAAACCTGC CCCCAAGACA
    CCTCCGAAGG CAGCAATGCC CCCTCAGATC ATCCAGTTCC CTGAGGACCA GAAGGTACGC
    GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA GTGACAGGCA CTCAGCCCAT CACCTGTACC
    TGGATGAAGT TCCGAAAGCA GATCCAGGAA AGCGAGCACA TGAAGGTGGA GAACAGCGAG
    AATGGCAGCA AGCTCACCAT CCTGGCCGCG CGCCAGGAGC ACTGCGGCTG CTACACACTG
    CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG GCCCAGGTCA ACCTCACTGT CGTGGATAAG
    CCAGACCCCC CAGCTGGCAC ACCTTGTGCC TCTGACATTC GGAGCTCCTC ACTGACCCTG
    TCCTGGTATG GCTCCTCATA TGATGGGGGC AGTGCTGTAC AGTCCTACAG CATCGAGATC
    TGGGACTCAG CCAACAAGAC GTGGAAGGAA CTAGCCACAT GCCGCAGCAC CTCTTTCAAC
    GTCCAGGACC TGCTGCCTGA CCACGAATAT AAGTTCCGTG TACGTGCAAT CAACGTGTAT
    GGAACCAGTG AGCCAAGCCA GGAGTCTGAA CTCACAACGG TAGGAGAGAA ACCTGAAGAG
    CCGAAGGATG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCCGAGGT TGATTACCGG
    ACAGTGACAA TCAATACTGA ACAAAAAGTA TCTGACTTCT ACGACATTGA GGAGAGATTA
    GGATCCGTGT CAGGAGGGGA GCTGTTTGAG CGCATCATTG ACGAGGACTT TGAGCTGACG
    GAGCGTGAGT GCATCAAGTA CATGCGGCAG ATCTCGGAGG GAGTGGAGTA CATCCACAAG
    CAGGGCATCG TGCACCTGGA CCTCAAGCCG GAGAACATCA TGTGTGTCAA CAAGACGGGC
    ACCAGGATCA AGCTCATCGA CTTTGGTCTG GCCAGGAGGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGCACCCC AGAATTTGTG GCTCCTGAAG TGATCAACTA TGAGCCCATC
    GGCTACGCCA CAGACATGTG GAGCATCGGG GTCATCTGCT ACATCCTAGT CAGTGGCCTT
    TCCCCCTTCA TGGGAGACAA CGATAACGAA ACCTTGGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGACG ACGAGGCATT CGATGAGATC TCCGACGATG CCAAGGATTT CATCAGCAAT
    CTGCTGAAGA AAGATATGAA AAACCGCCTG GACTGCACGC AGTGCCTTCA GCATCCATGG
    CTAATGAAAG ATACCAAGAA CATGGAGGCC AAGAAACTCT CCAAGGACCG GATGAAGAAG
    TACATGGCAA GAAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT TGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG GTCACCAACC
    AGCCCGCTCA ATGCAGAAAA ACTAGAATCT GAAGAAGATG TGTCCCAAGC TTTCCTTGAG
    GCTGTTGCTG AGGAAAAGCC TCATGTAAAA CCCTATTTCT CTAAGACCAT TCGCGATTTA
    GAAGTTGTGG AGGGAAGTGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCC
    GAGGTTGTCT GGTTCAAAGA TGACCAGTCA ATCAGGGAGT CCCGCCACTT CCAGATAGAC
    TACGATGAGG ACGGGAACTG CTCTTTAATT ATTAGTGATG TTTGCGGGGA TGACGATGCC
    AAGTACACCT GCAAGGCTGT CAACAGTCTT GGAGAAGCCA CCTGCACAGC AGAGCTCATT
    GTGGAAACGA TGGAGGAAGG TGAAGGGGAA GGGGAAGAGG AAGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511163.2
    CDS34..5604
    Translation

    Target ORF information:

    RefSeq Version XM_011512861.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011512861.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCACCTG GCCAGCTGCC CAAGCAGACC ATGGGGGATG TGAAGCTGGT TGCCTCGTCA 
    CACATTTCCA AAACCTCCCT CAGTGTGGAT CCCTCAAGAG TTGACTCCAT GCCCCTGACA
    GAGGCCCCTG CTTTCATTTT GCCCCCTCGG AACCTCTGCA TCAAAGAAGG AGCCACCGCC
    AAGTTCGAAG GGCGGGTCCG GGGTTACCCA GAGCCCCAGG TGACATGGCA CAGAAACGGG
    CAACCCATCA CCAGCGGGGG CCGCTTCCTG CTGGATTGCG GCATCCGGGG GACTTTCAGC
    CTTGTGATTC ATGCTGTCCA TGAGGAGGAC AGGGGAAAGT ATACCTGTGA AGCCACCAAT
    GGCAGTGGTG CTCGCCAGGT GACAGTGGAG TTGACAGTAG AAGGAAGTTT TGCGAAGCAG
    CTTGGTCAGC CTGTTGTTTC CAAAACCTTA GGGGATAGAT TTTCAGCTCC AGCAGTGGAG
    ACCCGTCCTA GCATCTGGGG GGAGTGCCCA CCAAAGTTTG CTACCAAGCT GGGCCGAGTT
    GTGGTCAAAG AAGGACAGAT GGGACGATTC TCCTGCAAGA TCACTGGCCG GCCCCAACCG
    CAGGTCACCT GGCTCAAGGG AAATGTTCCA CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT
    GAGAAGAACG GCATGCAGGT TCTGGAAATC CATGGAGTCA ACCAAGATGA CGTGGGAGTG
    TACACGTGCC TGGTGGTGAA CGGGTCGGGG AAGGCCTCGA TGTCAGCTGA ACTTTCCATC
    CAAGGTTTGG ACAGTGCCAA TAGGTCATTT GTGAGAGAAA CAAAAGCCAC CAATTCAGAT
    GTCAGGAAAG AGGTGACCAA TGTAATCTCA AAGGAGTCGA AGCTGGACAG TCTGGAGGCT
    GCAGCCAAAA GCAAGAACTG CTCCAGCCCC CAGAGAGGTG GCTCCCCACC CTGGGCTGCA
    AACAGCCAGC CTCAGCCCCC AAGGGAGTCC AAGCTGGAGT CATGCAAGGA CTCGCCCAGA
    ACGGCCCCGC AGACCCCGGT CCTTCAGAAG ACTTCCAGCT CCATCACCCT GCAGGCCGCA
    AGAGTTCAGC CGGAACCAAG AGCACCAGGC CTGGGGGTCC TATCACCTTC TGGAGAAGAG
    AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG
    AGCCAAGATG TTGTGAGCAA GGCTGCTAAC AGGAGAATCC CCATGGAGGG CCAGAGGGAT
    TCAGCATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAAGGA AAATCAAACT
    GTCAAGTTCA GATGTGAAGT TTCCGGGATT CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA
    GGCACCCCCG TGAGGAGACA GGAAGGCAGC ATTGAGGTTT ATGAAGATGC TGGCTCCCAT
    TACCTCTGCC TGCTGAAAGC CCGGACCAGG GACAGTGGGA CATACAGCTG CACTGCTTCC
    AACGCCCAAG GCCAGCTGTC CTGTAGCTGG ACCCTCCAAG TGGAAAGGCT TGCCGTGATG
    GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG AAGGACTGCG CTGTTATTGA GGGCCAGGAT
    TTTGTGCTGC AGTGCTCCGT ACGGGGGACC CCAGTGCCCC GGATCACTTG GCTGCTGAAT
    GGGCAGCCCA TCCAGTACGC TCGCTCCACC TGCGAGGCCG GCGTGGCTGA GCTCCACATC
    CAGGATGCCC TGCCGGAGGA CCATGGCACC TACACCTGCC TAGCTGAGAA TGCCTTGGGG
    CAGGTGTCCT GCAGCGCCTG GGTCACCGTC CATGAAAAGA AGAGTAGCAG GAAGAGTGAG
    TACCTTCTGC CTGTGGCTCC CAGCAAGCCC ACTGCACCCA TCTTCCTGCA GGGCCTCTCT
    GATCTCAAAG TCATGGATGG AAGCCAGGTC ACTATGACTG TCCAAGTGTC AGGGAATCCA
    CCCCCTGAAG TCATCTGGCT GCACAATGGG AATGAGATCC AAGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGAACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC GGAGGACACG
    GGCACGTACA CCTGCGAGGC CTGGAACAGC GCTGGAGAGG TCCGCACCCA GGCCGTGCTC
    ACGGTACAAG AGCCTCACGA TGGCACCCAG CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG
    ACAGCCTCCC TGGGCCAGAG TGTCCTCATC TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT
    ACCGTGCACT GGCTCAGAGA TGGCAAAGCC CTCTGCAAAG ACACTGGCCA CTTCGAGGTG
    CTTCAGAATG AGGACGTGTT CACCCTGGTT CTAAAGAAGG TGCAGCCCTG GCATGCCGGC
    CAGTATGAGA TCCTGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAACA GCTCTGCCAG AGCCCTTCCA CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC
    CTCTGTGGTG GAGGAGTTGG TGCTGATGGT GGTGGTAGTG ACCGCTATGG GTCCCTGAGG
    CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG CTAGAGGAGG AAGACGGCGA GGACGTGCGA
    GGGGTGCTGA AGAGGCGCGT GGAGACGAGG CAGCACACTG AGGAGGCGAT CCGCCAGCAG
    GAGGTGGAGC AGCTGGACTT CCGAGACCTC CTGGGGAAGA AGGTGAGTAC AAAGACCCTA
    TCGGAAGACG ACCTGAAGGA GATCCCAGCC GAGCAGATGG ATTTCCGTGC CAACCTGCAG
    CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG GAAGAGAGGA AGGTGCACAG CCCCCAGCAG
    GTCGATTTTC GCTCTGTCCT GGCCAAGAAG GGGACTTCCA AGACCCCCGT GCCTGAGAAG
    GTGCCACCGC CAAAACCTGC CACCCCGGAT TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA
    TTACCAGCAG AGAATGGCAG CAGCAGTGCC GAGACCCTGA ATGCCAAGGC AGTGGAGAGT
    TCCAAGCCCC TGAGCAATGC ACAGCCTTCA GGGCCCTTGA AACCCGTGGG CAACGCCAAG
    CCTGCTGAGA CCCTGAAGCC AATGGGCAAC GCCAAGCCTG CCGAGACCCT GAAGCCCATG
    GGCAATGCCA AGCCTGATGA GAACCTGAAA TCCGCTAGCA AAGAAGAACT CAAGAAAGAC
    GTTAAGAATG ATGTGAACTG CAAGAGAGGC CATGCAGGGA CCACAGATAA TGAAAAGAGA
    TCAGAGAGCC AGGGGACAGC CCCAGCCTTC AAGCAGAAGC TGCAAGATGT TCATGTGGCA
    GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG GTGTCTTCTG ACCCCCCAGC CACCATCATC
    TGGACGCTGA ACGGAAAGAC CCTCAAGACC ACCAAGTTCA TCATCCTCTC CCAGGAAGGC
    TCACTCTGCT CCGTCTCCAT CGAGAAGGCA CTGCCTGAGG ACAGAGGCTT ATACAAGTGT
    GTAGCCAAGA ATGACGCTGG CCAGGCGGAG TGCTCCTGCC AAGTCACCGT GGATGATGCT
    CCAGCCAGTG AGAACACCAA GGCCCCAGAG ATGAAATCCC GGAGGCCCAA GAGCTCTCTT
    CCTCCCGTGC TAGGAACTGA GAGTGATGCG ACTGTGAAAA AGAAACCTGC CCCCAAGACA
    CCTCCGAAGG CAGCAATGCC CCCTCAGATC ATCCAGTTCC CTGAGGACCA GAAGGTACGC
    GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA GTGACAGGCA CTCAGCCCAT CACCTGTACC
    TGGATGAAGT TCCGAAAGCA GATCCAGGAA AGCGAGCACA TGAAGGTGGA GAACAGCGAG
    AATGGCAGCA AGCTCACCAT CCTGGCCGCG CGCCAGGAGC ACTGCGGCTG CTACACACTG
    CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG GCCCAGGTCA ACCTCACTGT CGTGGATAAG
    CCAGACCCCC CAGCTGGCAC ACCTTGTGCC TCTGACATTC GGAGCTCCTC ACTGACCCTG
    TCCTGGTATG GCTCCTCATA TGATGGGGGC AGTGCTGTAC AGTCCTACAG CATCGAGATC
    TGGGACTCAG CCAACAAGAC GTGGAAGGAA CTAGCCACAT GCCGCAGCAC CTCTTTCAAC
    GTCCAGGACC TGCTGCCTGA CCACGAATAT AAGTTCCGTG TACGTGCAAT CAACGTGTAT
    GGAACCAGTG AGCCAAGCCA GGAGTCTGAA CTCACAACGG TAGGAGAGAA ACCTGAAGAG
    CCGAAGGATG AAGTGGAGGT GTCAGATGAT GATGAGAAGG AGCCCGAGGT TGATTACCGG
    ACAGTGACAA TCAATACTGA ACAAAAAGTA TCTGACTTCT ACGACATTGA GGAGAGATTA
    GGATCCGTGT CAGGAGGGGA GCTGTTTGAG CGCATCATTG ACGAGGACTT TGAGCTGACG
    GAGCGTGAGT GCATCAAGTA CATGCGGCAG ATCTCGGAGG GAGTGGAGTA CATCCACAAG
    CAGGGCATCG TGCACCTGGA CCTCAAGCCG GAGAACATCA TGTGTGTCAA CAAGACGGGC
    ACCAGGATCA AGCTCATCGA CTTTGGTCTG GCCAGGAGGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGCACCCC AGAATTTGTG GCTCCTGAAG TGATCAACTA TGAGCCCATC
    GGCTACGCCA CAGACATGTG GAGCATCGGG GTCATCTGCT ACATCCTAGT CAGTGGCCTT
    TCCCCCTTCA TGGGAGACAA CGATAACGAA ACCTTGGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGACG ACGAGGCATT CGATGAGATC TCCGACGATG CCAAGGATTT CATCAGCAAT
    CTGCTGAAGA AAGATATGAA AAACCGCCTG GACTGCACGC AGTGCCTTCA GCATCCATGG
    CTAATGAAAG ATACCAAGAA CATGGAGGCC AAGAAACTCT CCAAGGACCG GATGAAGAAG
    TACATGGCAA GAAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT TGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG GTCACCAACC
    AGCCCGCTCA ATGCAGAAAA ACTAGAATCT GAAGAAGATG TGTCCCAAGC TTTCCTTGAG
    GCTGTTGCTG AGGAAAAGCC TCATGTAAAA CCCTATTTCT CTAAGACCAT TCGCGATTTA
    GAAGTTGTGG AGGGAAGTGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCC
    GAGGTTGTCT GGTTCAAAGA TGACCAGTCA ATCAGGGAGT CCCGCCACTT CCAGATAGAC
    TACGATGAGG ACGGGAACTG CTCTTTAATT ATTAGTGATG TTTGCGGGGA TGACGATGCC
    AAGTACACCT GCAAGGCTGT CAACAGTCTT GGAGAAGCCA CCTGCACAGC AGAGCTCATT
    GTGGAAACGA TGGAGGAAGG TGAAGGGGAA GGGGAAGAGG AAGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105372
    Clone ID Related Accession (Same CDS sequence) XM_024453534.1
    Accession Version XM_024453534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCACCTG GCCAGCTGCC CAAGCAGACC ATGGGGGATG TGAAGCTGGT TGCCTCGTCA 
    CACATTTCCA AAACCTCCCT CAGTGTGGAT CCCTCAAGAG TTGACTCCAT GCCCCTGACA
    GAGGCCCCTG CTTTCATTTT GCCCCCTCGG AACCTCTGCA TCAAAGAAGG AGCCACCGCC
    AAGTTCGAAG GGCGGGTCCG GGGTTACCCA GAGCCCCAGG TGACATGGCA CAGAAACGGG
    CAACCCATCA CCAGCGGGGG CCGCTTCCTG CTGGATTGCG GCATCCGGGG GACTTTCAGC
    CTTGTGATTC ATGCTGTCCA TGAGGAGGAC AGGGGAAAGT ATACCTGTGA AGCCACCAAT
    GGCAGTGGTG CTCGCCAGGT GACAGTGGAG TTGACAGTAG AAGGAAGTTT TGCGAAGCAG
    CTTGGTCAGC CTGTTGTTTC CAAAACCTTA GGGGATAGAT TTTCAGCTCC AGCAGTGGAG
    ACCCGTCCTA GCATCTGGGG GGAGTGCCCA CCAAAGTTTG CTACCAAGCT GGGCCGAGTT
    GTGGTCAAAG AAGGACAGAT GGGACGATTC TCCTGCAAGA TCACTGGCCG GCCCCAACCG
    CAGGTCACCT GGCTCAAGGG AAATGTTCCA CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT
    GAGAAGAACG GCATGCAGGT TCTGGAAATC CATGGAGTCA ACCAAGATGA CGTGGGAGTG
    TACACGTGCC TGGTGGTGAA CGGGTCGGGG AAGGCCTCGA TGTCAGCTGA ACTTTCCATC
    CAAGGTTTGG ACAGTGCCAA TAGGTCATTT GTGAGAGAAA CAAAAGCCAC CAATTCAGAT
    GTCAGGAAAG AGGTGACCAA TGTAATCTCA AAGGAGTCGA AGCTGGACAG TCTGGAGGCT
    GCAGCCAAAA GCAAGAACTG CTCCAGCCCC CAGAGAGGTG GCTCCCCACC CTGGGCTGCA
    AACAGCCAGC CTCAGCCCCC AAGGGAGTCC AAGCTGGAGT CATGCAAGGA CTCGCCCAGA
    ACGGCCCCGC AGACCCCGGT CCTTCAGAAG ACTTCCAGCT CCATCACCCT GCAGGCCGCA
    AGAGTTCAGC CGGAACCAAG AGCACCAGGC CTGGGGGTCC TATCACCTTC TGGAGAAGAG
    AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG
    AGCCAAGATG TTGTGAGCAA GGCTGCTAAC AGGAGAATCC CCATGGAGGG CCAGAGGGAT
    TCAGCATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAAGGA AAATCAAACT
    GTCAAGTTCA GATGTGAAGG GCTTGCCGTG ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC
    CTGAAGGACT GCGCTGTTAT TGAGGGCCAG GATTTTGTGC TGCAGTGCTC CGTACGGGGG
    ACCCCAGTGC CCCGGATCAC TTGGCTGCTG AATGGGCAGC CCATCCAGTA CGCTCGCTCC
    ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC ATCCAGGATG CCCTGCCGGA GGACCATGGC
    ACCTACACCT GCCTAGCTGA GAATGCCTTG GGGCAGGTGT CCTGCAGCGC CTGGGTCACC
    GTCCATGAAA AGAAGAGTAG CAGGAAGAGT GAGTACCTTC TGCCTGTGGC TCCCAGCAAG
    CCCACTGCAC CCATCTTCCT GCAGGGCCTC TCTGATCTCA AAGTCATGGA TGGAAGCCAG
    GTCACTATGA CTGTCCAAGT GTCAGGGAAT CCACCCCCTG AAGTCATCTG GCTGCACAAT
    GGGAATGAGA TCCAAGAGTC AGAGGACTTC CACTTTGAAC AGAGAGGAAC TCAGCACAGC
    CTTTGTATCC AGGAAGTGTT CCCGGAGGAC ACGGGCACGT ACACCTGCGA GGCCTGGAAC
    AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG CTCACGGTAC AAGAGCCTCA CGATGGCACC
    CAGCCCTGGT TCATCAGTAA GCCTCGCTCA GTGACAGCCT CCCTGGGCCA GAGTGTCCTC
    ATCTCCTGCG CCATAGCTGG TGACCCCTTT CCTACCGTGC ACTGGCTCAG AGATGGCAAA
    GCCCTCTGCA AAGACACTGG CCACTTCGAG GTGCTTCAGA ATGAGGACGT GTTCACCCTG
    GTTCTAAAGA AGGTGCAGCC CTGGCATGCC GGCCAGTATG AGATCCTGCT CAAGAACCGG
    GTTGGCGAAT GCAGTTGCCA GGTGTCACTG ATGCTACAGA ACAGCTCTGC CAGAGCCCTT
    CCACGGGGGA GGGAGCCTGC CAGCTGCGAG GACCTCTGTG GTGGAGGAGT TGGTGCTGAT
    GGTGGTGGTA GTGACCGCTA TGGGTCCCTG AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT
    TGGCTAGAGG AGGAAGACGG CGAGGACGTG CGAGGGGTGC TGAAGAGGCG CGTGGAGACG
    AGGCAGCACA CTGAGGAGGC GATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC
    CTCCTGGGGA AGAAGGTGAG TACAAAGACC CTATCGGAAG ACGACCTGAA GGAGATCCCA
    GCCGAGCAGA TGGATTTCCG TGCCAACCTG CAGCGGCAAG TGAAGCCAAA GACTGTGTCT
    GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG CAGGTCGATT TTCGCTCTGT CCTGGCCAAG
    AAGGGGACTT CCAAGACCCC CGTGCCTGAG AAGGTGCCAC CGCCAAAACC TGCCACCCCG
    GATTTTCGCT CAGTGCTGGG TGGCAAGAAG AAATTACCAG CAGAGAATGG CAGCAGCAGT
    GCCGAGACCC TGAATGCCAA GGCAGTGGAG AGTTCCAAGC CCCTGAGCAA TGCACAGCCT
    TCAGGGCCCT TGAAACCCGT GGGCAACGCC AAGCCTGCTG AGACCCTGAA GCCAATGGGC
    AACGCCAAGC CTGCCGAGAC CCTGAAGCCC ATGGGCAATG CCAAGCCTGA TGAGAACCTG
    AAATCCGCTA GCAAAGAAGA ACTCAAGAAA GACGTTAAGA ATGATGTGAA CTGCAAGAGA
    GGCCATGCAG GGACCACAGA TAATGAAAAG AGATCAGAGA GCCAGGGGAC AGCCCCAGCC
    TTCAAGCAGA AGCTGCAAGA TGTTCATGTG GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC
    CAGGTGTCTT CTGACCCCCC AGCCACCATC ATCTGGACGC TGAACGGAAA GACCCTCAAG
    ACCACCAAGT TCATCATCCT CTCCCAGGAA GGCTCACTCT GCTCCGTCTC CATCGAGAAG
    GCACTGCCTG AGGACAGAGG CTTATACAAG TGTGTAGCCA AGAATGACGC TGGCCAGGCG
    GAGTGCTCCT GCCAAGTCAC CGTGGATGAT GCTCCAGCCA GTGAGAACAC CAAGGCCCCA
    GAGATGAAAT CCCGGAGGCC CAAGAGCTCT CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT
    GCGACTGTGA AAAAGAAACC TGCCCCCAAG ACACCTCCGA AGGCAGCAAT GCCCCCTCAG
    ATCATCCAGT TCCCTGAGGA CCAGAAGGTA CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC
    AAAGTGACAG GCACTCAGCC CATCACCTGT ACCTGGATGA AGTTCCGAAA GCAGATCCAG
    GAAAGCGAGC ACATGAAGGT GGAGAACAGC GAGAATGGCA GCAAGCTCAC CATCCTGGCC
    GCGCGCCAGG AGCACTGCGG CTGCTACACA CTGCTGGTGG AGAACAAGCT GGGCAGCAGG
    CAGGCCCAGG TCAACCTCAC TGTCGTGGAT AAGCCAGACC CCCCAGCTGG CACACCTTGT
    GCCTCTGACA TTCGGAGCTC CTCACTGACC CTGTCCTGGT ATGGCTCCTC ATATGATGGG
    GGCAGTGCTG TACAGTCCTA CAGCATCGAG ATCTGGGACT CAGCCAACAA GACGTGGAAG
    GAACTAGCCA CATGCCGCAG CACCTCTTTC AACGTCCAGG ACCTGCTGCC TGACCACGAA
    TATAAGTTCC GTGTACGTGC AATCAACGTG TATGGAACCA GTGAGCCAAG CCAGGAGTCT
    GAACTCACAA CGGTAGGAGA GAAACCTGAA GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT
    GATGATGAGA AGGAGCCCGA GGTTGATTAC CGGACAGTGA CAATCAATAC TGAACAAAAA
    GTATCTGACT TCTACGACAT TGAGGAGAGA TTAGGATCTG GGAAATTTGG ACAGGTCTTT
    CGACTTGTAG AAAAGAAAAC TCGAAAAGTC TGGGCAGGGA AGTTCTTCAA GGCATATTCA
    GCAAAAGAGA AAGAGAATAT CCGGCAGGAG ATTAGCATCA TGAACTGCCT CCACCACCCT
    AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA GAAAAGGCCA ACATCGTCAT GGTCCTGGAG
    ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC ATCATTGACG AGGACTTTGA GCTGACGGAG
    CGTGAGTGCA TCAAGTACAT GCGGCAGATC TCGGAGGGAG TGGAGTACAT CCACAAGCAG
    GGCATCGTGC ACCTGGACCT CAAGCCGGAG AACATCATGT GTGTCAACAA GACGGGCACC
    AGGATCAAGC TCATCGACTT TGGTCTGGCC AGGAGGCTGG AGAATGCGGG GTCTCTGAAG
    GTCCTCTTTG GCACCCCAGA ATTTGTGGCT CCTGAAGTGA TCAACTATGA GCCCATCGGC
    TACGCCACAG ACATGTGGAG CATCGGGGTC ATCTGCTACA TCCTAGTCAG TGGCCTTTCC
    CCCTTCATGG GAGACAACGA TAACGAAACC TTGGCCAACG TTACCTCAGC CACCTGGGAC
    TTCGACGACG AGGCATTCGA TGAGATCTCC GACGATGCCA AGGATTTCAT CAGCAATCTG
    CTGAAGAAAG ATATGAAAAA CCGCCTGGAC TGCACGCAGT GCCTTCAGCA TCCATGGCTA
    ATGAAAGATA CCAAGAACAT GGAGGCCAAG AAACTCTCCA AGGACCGGAT GAAGAAGTAC
    ATGGCAAGAA GGAAATGGCA GAAAACGGGC AATGCTGTGA GAGCCATTGG AAGACTGTCC
    TCTATGGCAA TGATCTCAGG GCTCAGTGGC AGGAAATCCT CAACAGGGTC ACCAACCAGC
    CCGCTCAATG CAGAAAAACT AGAATCTGAA GAAGATGTGT CCCAAGCTTT CCTTGAGGCT
    GTTGCTGAGG AAAAGCCTCA TGTAAAACCC TATTTCTCTA AGACCATTCG CGATTTAGAA
    GTTGTGGAGG GAAGTGCTGC TAGATTTGAC TGCAAGATTG AAGGATACCC AGACCCCGAG
    GTTGTCTGGT TCAAAGATGA CCAGTCAATC AGGGAGTCCC GCCACTTCCA GATAGACTAC
    GATGAGGACG GGAACTGCTC TTTAATTATT AGTGATGTTT GCGGGGATGA CGATGCCAAG
    TACACCTGCA AGGCTGTCAA CAGTCTTGGA GAAGCCACCT GCACAGCAGA GCTCATTGTG
    GAAACGATGG AGGAAGGTGA AGGGGAAGGG GAAGAGGAAG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309302.1
    CDS29..5596
    Translation

    Target ORF information:

    RefSeq Version XM_024453534.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCACCTG GCCAGCTGCC CAAGCAGACC ATGGGGGATG TGAAGCTGGT TGCCTCGTCA 
    CACATTTCCA AAACCTCCCT CAGTGTGGAT CCCTCAAGAG TTGACTCCAT GCCCCTGACA
    GAGGCCCCTG CTTTCATTTT GCCCCCTCGG AACCTCTGCA TCAAAGAAGG AGCCACCGCC
    AAGTTCGAAG GGCGGGTCCG GGGTTACCCA GAGCCCCAGG TGACATGGCA CAGAAACGGG
    CAACCCATCA CCAGCGGGGG CCGCTTCCTG CTGGATTGCG GCATCCGGGG GACTTTCAGC
    CTTGTGATTC ATGCTGTCCA TGAGGAGGAC AGGGGAAAGT ATACCTGTGA AGCCACCAAT
    GGCAGTGGTG CTCGCCAGGT GACAGTGGAG TTGACAGTAG AAGGAAGTTT TGCGAAGCAG
    CTTGGTCAGC CTGTTGTTTC CAAAACCTTA GGGGATAGAT TTTCAGCTCC AGCAGTGGAG
    ACCCGTCCTA GCATCTGGGG GGAGTGCCCA CCAAAGTTTG CTACCAAGCT GGGCCGAGTT
    GTGGTCAAAG AAGGACAGAT GGGACGATTC TCCTGCAAGA TCACTGGCCG GCCCCAACCG
    CAGGTCACCT GGCTCAAGGG AAATGTTCCA CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT
    GAGAAGAACG GCATGCAGGT TCTGGAAATC CATGGAGTCA ACCAAGATGA CGTGGGAGTG
    TACACGTGCC TGGTGGTGAA CGGGTCGGGG AAGGCCTCGA TGTCAGCTGA ACTTTCCATC
    CAAGGTTTGG ACAGTGCCAA TAGGTCATTT GTGAGAGAAA CAAAAGCCAC CAATTCAGAT
    GTCAGGAAAG AGGTGACCAA TGTAATCTCA AAGGAGTCGA AGCTGGACAG TCTGGAGGCT
    GCAGCCAAAA GCAAGAACTG CTCCAGCCCC CAGAGAGGTG GCTCCCCACC CTGGGCTGCA
    AACAGCCAGC CTCAGCCCCC AAGGGAGTCC AAGCTGGAGT CATGCAAGGA CTCGCCCAGA
    ACGGCCCCGC AGACCCCGGT CCTTCAGAAG ACTTCCAGCT CCATCACCCT GCAGGCCGCA
    AGAGTTCAGC CGGAACCAAG AGCACCAGGC CTGGGGGTCC TATCACCTTC TGGAGAAGAG
    AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG
    AGCCAAGATG TTGTGAGCAA GGCTGCTAAC AGGAGAATCC CCATGGAGGG CCAGAGGGAT
    TCAGCATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAAGGA AAATCAAACT
    GTCAAGTTCA GATGTGAAGG GCTTGCCGTG ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC
    CTGAAGGACT GCGCTGTTAT TGAGGGCCAG GATTTTGTGC TGCAGTGCTC CGTACGGGGG
    ACCCCAGTGC CCCGGATCAC TTGGCTGCTG AATGGGCAGC CCATCCAGTA CGCTCGCTCC
    ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC ATCCAGGATG CCCTGCCGGA GGACCATGGC
    ACCTACACCT GCCTAGCTGA GAATGCCTTG GGGCAGGTGT CCTGCAGCGC CTGGGTCACC
    GTCCATGAAA AGAAGAGTAG CAGGAAGAGT GAGTACCTTC TGCCTGTGGC TCCCAGCAAG
    CCCACTGCAC CCATCTTCCT GCAGGGCCTC TCTGATCTCA AAGTCATGGA TGGAAGCCAG
    GTCACTATGA CTGTCCAAGT GTCAGGGAAT CCACCCCCTG AAGTCATCTG GCTGCACAAT
    GGGAATGAGA TCCAAGAGTC AGAGGACTTC CACTTTGAAC AGAGAGGAAC TCAGCACAGC
    CTTTGTATCC AGGAAGTGTT CCCGGAGGAC ACGGGCACGT ACACCTGCGA GGCCTGGAAC
    AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG CTCACGGTAC AAGAGCCTCA CGATGGCACC
    CAGCCCTGGT TCATCAGTAA GCCTCGCTCA GTGACAGCCT CCCTGGGCCA GAGTGTCCTC
    ATCTCCTGCG CCATAGCTGG TGACCCCTTT CCTACCGTGC ACTGGCTCAG AGATGGCAAA
    GCCCTCTGCA AAGACACTGG CCACTTCGAG GTGCTTCAGA ATGAGGACGT GTTCACCCTG
    GTTCTAAAGA AGGTGCAGCC CTGGCATGCC GGCCAGTATG AGATCCTGCT CAAGAACCGG
    GTTGGCGAAT GCAGTTGCCA GGTGTCACTG ATGCTACAGA ACAGCTCTGC CAGAGCCCTT
    CCACGGGGGA GGGAGCCTGC CAGCTGCGAG GACCTCTGTG GTGGAGGAGT TGGTGCTGAT
    GGTGGTGGTA GTGACCGCTA TGGGTCCCTG AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT
    TGGCTAGAGG AGGAAGACGG CGAGGACGTG CGAGGGGTGC TGAAGAGGCG CGTGGAGACG
    AGGCAGCACA CTGAGGAGGC GATCCGCCAG CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC
    CTCCTGGGGA AGAAGGTGAG TACAAAGACC CTATCGGAAG ACGACCTGAA GGAGATCCCA
    GCCGAGCAGA TGGATTTCCG TGCCAACCTG CAGCGGCAAG TGAAGCCAAA GACTGTGTCT
    GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG CAGGTCGATT TTCGCTCTGT CCTGGCCAAG
    AAGGGGACTT CCAAGACCCC CGTGCCTGAG AAGGTGCCAC CGCCAAAACC TGCCACCCCG
    GATTTTCGCT CAGTGCTGGG TGGCAAGAAG AAATTACCAG CAGAGAATGG CAGCAGCAGT
    GCCGAGACCC TGAATGCCAA GGCAGTGGAG AGTTCCAAGC CCCTGAGCAA TGCACAGCCT
    TCAGGGCCCT TGAAACCCGT GGGCAACGCC AAGCCTGCTG AGACCCTGAA GCCAATGGGC
    AACGCCAAGC CTGCCGAGAC CCTGAAGCCC ATGGGCAATG CCAAGCCTGA TGAGAACCTG
    AAATCCGCTA GCAAAGAAGA ACTCAAGAAA GACGTTAAGA ATGATGTGAA CTGCAAGAGA
    GGCCATGCAG GGACCACAGA TAATGAAAAG AGATCAGAGA GCCAGGGGAC AGCCCCAGCC
    TTCAAGCAGA AGCTGCAAGA TGTTCATGTG GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC
    CAGGTGTCTT CTGACCCCCC AGCCACCATC ATCTGGACGC TGAACGGAAA GACCCTCAAG
    ACCACCAAGT TCATCATCCT CTCCCAGGAA GGCTCACTCT GCTCCGTCTC CATCGAGAAG
    GCACTGCCTG AGGACAGAGG CTTATACAAG TGTGTAGCCA AGAATGACGC TGGCCAGGCG
    GAGTGCTCCT GCCAAGTCAC CGTGGATGAT GCTCCAGCCA GTGAGAACAC CAAGGCCCCA
    GAGATGAAAT CCCGGAGGCC CAAGAGCTCT CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT
    GCGACTGTGA AAAAGAAACC TGCCCCCAAG ACACCTCCGA AGGCAGCAAT GCCCCCTCAG
    ATCATCCAGT TCCCTGAGGA CCAGAAGGTA CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC
    AAAGTGACAG GCACTCAGCC CATCACCTGT ACCTGGATGA AGTTCCGAAA GCAGATCCAG
    GAAAGCGAGC ACATGAAGGT GGAGAACAGC GAGAATGGCA GCAAGCTCAC CATCCTGGCC
    GCGCGCCAGG AGCACTGCGG CTGCTACACA CTGCTGGTGG AGAACAAGCT GGGCAGCAGG
    CAGGCCCAGG TCAACCTCAC TGTCGTGGAT AAGCCAGACC CCCCAGCTGG CACACCTTGT
    GCCTCTGACA TTCGGAGCTC CTCACTGACC CTGTCCTGGT ATGGCTCCTC ATATGATGGG
    GGCAGTGCTG TACAGTCCTA CAGCATCGAG ATCTGGGACT CAGCCAACAA GACGTGGAAG
    GAACTAGCCA CATGCCGCAG CACCTCTTTC AACGTCCAGG ACCTGCTGCC TGACCACGAA
    TATAAGTTCC GTGTACGTGC AATCAACGTG TATGGAACCA GTGAGCCAAG CCAGGAGTCT
    GAACTCACAA CGGTAGGAGA GAAACCTGAA GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT
    GATGATGAGA AGGAGCCCGA GGTTGATTAC CGGACAGTGA CAATCAATAC TGAACAAAAA
    GTATCTGACT TCTACGACAT TGAGGAGAGA TTAGGATCTG GGAAATTTGG ACAGGTCTTT
    CGACTTGTAG AAAAGAAAAC TCGAAAAGTC TGGGCAGGGA AGTTCTTCAA GGCATATTCA
    GCAAAAGAGA AAGAGAATAT CCGGCAGGAG ATTAGCATCA TGAACTGCCT CCACCACCCT
    AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA GAAAAGGCCA ACATCGTCAT GGTCCTGGAG
    ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC ATCATTGACG AGGACTTTGA GCTGACGGAG
    CGTGAGTGCA TCAAGTACAT GCGGCAGATC TCGGAGGGAG TGGAGTACAT CCACAAGCAG
    GGCATCGTGC ACCTGGACCT CAAGCCGGAG AACATCATGT GTGTCAACAA GACGGGCACC
    AGGATCAAGC TCATCGACTT TGGTCTGGCC AGGAGGCTGG AGAATGCGGG GTCTCTGAAG
    GTCCTCTTTG GCACCCCAGA ATTTGTGGCT CCTGAAGTGA TCAACTATGA GCCCATCGGC
    TACGCCACAG ACATGTGGAG CATCGGGGTC ATCTGCTACA TCCTAGTCAG TGGCCTTTCC
    CCCTTCATGG GAGACAACGA TAACGAAACC TTGGCCAACG TTACCTCAGC CACCTGGGAC
    TTCGACGACG AGGCATTCGA TGAGATCTCC GACGATGCCA AGGATTTCAT CAGCAATCTG
    CTGAAGAAAG ATATGAAAAA CCGCCTGGAC TGCACGCAGT GCCTTCAGCA TCCATGGCTA
    ATGAAAGATA CCAAGAACAT GGAGGCCAAG AAACTCTCCA AGGACCGGAT GAAGAAGTAC
    ATGGCAAGAA GGAAATGGCA GAAAACGGGC AATGCTGTGA GAGCCATTGG AAGACTGTCC
    TCTATGGCAA TGATCTCAGG GCTCAGTGGC AGGAAATCCT CAACAGGGTC ACCAACCAGC
    CCGCTCAATG CAGAAAAACT AGAATCTGAA GAAGATGTGT CCCAAGCTTT CCTTGAGGCT
    GTTGCTGAGG AAAAGCCTCA TGTAAAACCC TATTTCTCTA AGACCATTCG CGATTTAGAA
    GTTGTGGAGG GAAGTGCTGC TAGATTTGAC TGCAAGATTG AAGGATACCC AGACCCCGAG
    GTTGTCTGGT TCAAAGATGA CCAGTCAATC AGGGAGTCCC GCCACTTCCA GATAGACTAC
    GATGAGGACG GGAACTGCTC TTTAATTATT AGTGATGTTT GCGGGGATGA CGATGCCAAG
    TACACCTGCA AGGCTGTCAA CAGTCTTGGA GAAGCCACCT GCACAGCAGA GCTCATTGTG
    GAAACGATGG AGGAAGGTGA AGGGGAAGGG GAAGAGGAAG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16237
    Clone ID Related Accession (Same CDS sequence) NM_053027.3 , NM_053027.4
    Accession Version NM_053027.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 3A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC064695.1, AC117381.5, U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1. On Oct 10, 2006 this sequence version replaced NM_053027.2. Transcript Variant: This variant (3A) does not utilize exon 30, compared to variant 1, resulting in a shorter protein (isoform 3A), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF069602.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAAACCGCCT GGACTGCACG
    CAGTGCCTTC AGCATCCATG GCTAATGAAA GATACCAAGA ACATGGAGGC CAAGAAACTC
    TCCAAGGACC GGATGAAGAA GTACATGGCA AGAAGGAAAT GGCAGAAAAC GGGCAATGCT
    GTGAGAGCCA TTGGAAGACT GTCCTCTATG GCAATGATCT CAGGGCTCAG TGGCAGGAAA
    TCCTCAACAG GGTCACCAAC CAGCCCGCTC AATGCAGAAA AACTAGAATC TGAAGAAGAT
    GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT GAGGAAAAGC CTCATGTAAA ACCCTATTTC
    TCTAAGACCA TTCGCGATTT AGAAGTTGTG GAGGGAAGTG CTGCTAGATT TGACTGCAAG
    ATTGAAGGAT ACCCAGACCC CGAGGTTGTC TGGTTCAAAG ATGACCAGTC AATCAGGGAG
    TCCCGCCACT TCCAGATAGA CTACGATGAG GACGGGAACT GCTCTTTAAT TATTAGTGAT
    GTTTGCGGGG ATGACGATGC CAAGTACACC TGCAAGGCTG TCAACAGTCT TGGAGAAGCC
    ACCTGCACAG CAGAGCTCAT TGTGGAAACG ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG
    GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444255.3
    CDS283..5874
    Translation

    Target ORF information:

    RefSeq Version NM_053027.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 3A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053027.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAAACCGCCT GGACTGCACG
    CAGTGCCTTC AGCATCCATG GCTAATGAAA GATACCAAGA ACATGGAGGC CAAGAAACTC
    TCCAAGGACC GGATGAAGAA GTACATGGCA AGAAGGAAAT GGCAGAAAAC GGGCAATGCT
    GTGAGAGCCA TTGGAAGACT GTCCTCTATG GCAATGATCT CAGGGCTCAG TGGCAGGAAA
    TCCTCAACAG GGTCACCAAC CAGCCCGCTC AATGCAGAAA AACTAGAATC TGAAGAAGAT
    GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT GAGGAAAAGC CTCATGTAAA ACCCTATTTC
    TCTAAGACCA TTCGCGATTT AGAAGTTGTG GAGGGAAGTG CTGCTAGATT TGACTGCAAG
    ATTGAAGGAT ACCCAGACCC CGAGGTTGTC TGGTTCAAAG ATGACCAGTC AATCAGGGAG
    TCCCGCCACT TCCAGATAGA CTACGATGAG GACGGGAACT GCTCTTTAAT TATTAGTGAT
    GTTTGCGGGG ATGACGATGC CAAGTACACC TGCAAGGCTG TCAACAGTCT TGGAGAAGCC
    ACCTGCACAG CAGAGCTCAT TGTGGAAACG ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG
    GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16237
    Clone ID Related Accession (Same CDS sequence) NM_053027.3 , NM_053027.4
    Accession Version NM_053027.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 3A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117381.5, BC064695.1, U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1. On Jun 2, 2019 this sequence version replaced NM_053027.3. Transcript Variant: This variant (3A) does not utilize exon 30, compared to variant 1, resulting in a shorter protein (isoform 3A), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF069602.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAAACCGCCT GGACTGCACG
    CAGTGCCTTC AGCATCCATG GCTAATGAAA GATACCAAGA ACATGGAGGC CAAGAAACTC
    TCCAAGGACC GGATGAAGAA GTACATGGCA AGAAGGAAAT GGCAGAAAAC GGGCAATGCT
    GTGAGAGCCA TTGGAAGACT GTCCTCTATG GCAATGATCT CAGGGCTCAG TGGCAGGAAA
    TCCTCAACAG GGTCACCAAC CAGCCCGCTC AATGCAGAAA AACTAGAATC TGAAGAAGAT
    GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT GAGGAAAAGC CTCATGTAAA ACCCTATTTC
    TCTAAGACCA TTCGCGATTT AGAAGTTGTG GAGGGAAGTG CTGCTAGATT TGACTGCAAG
    ATTGAAGGAT ACCCAGACCC CGAGGTTGTC TGGTTCAAAG ATGACCAGTC AATCAGGGAG
    TCCCGCCACT TCCAGATAGA CTACGATGAG GACGGGAACT GCTCTTTAAT TATTAGTGAT
    GTTTGCGGGG ATGACGATGC CAAGTACACC TGCAAGGCTG TCAACAGTCT TGGAGAAGCC
    ACCTGCACAG CAGAGCTCAT TGTGGAAACG ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG
    GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444255.3
    CDS313..5904
    Translation

    Target ORF information:

    RefSeq Version NM_053027.4
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 3A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053027.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAAACCGCCT GGACTGCACG
    CAGTGCCTTC AGCATCCATG GCTAATGAAA GATACCAAGA ACATGGAGGC CAAGAAACTC
    TCCAAGGACC GGATGAAGAA GTACATGGCA AGAAGGAAAT GGCAGAAAAC GGGCAATGCT
    GTGAGAGCCA TTGGAAGACT GTCCTCTATG GCAATGATCT CAGGGCTCAG TGGCAGGAAA
    TCCTCAACAG GGTCACCAAC CAGCCCGCTC AATGCAGAAA AACTAGAATC TGAAGAAGAT
    GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT GAGGAAAAGC CTCATGTAAA ACCCTATTTC
    TCTAAGACCA TTCGCGATTT AGAAGTTGTG GAGGGAAGTG CTGCTAGATT TGACTGCAAG
    ATTGAAGGAT ACCCAGACCC CGAGGTTGTC TGGTTCAAAG ATGACCAGTC AATCAGGGAG
    TCCCGCCACT TCCAGATAGA CTACGATGAG GACGGGAACT GCTCTTTAAT TATTAGTGAT
    GTTTGCGGGG ATGACGATGC CAAGTACACC TGCAAGGCTG TCAACAGTCT TGGAGAAGCC
    ACCTGCACAG CAGAGCTCAT TGTGGAAACG ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG
    GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16318
    Clone ID Related Accession (Same CDS sequence) NM_053026.4 , NM_053026.3 , XM_024453535.1
    Accession Version NM_053026.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117381.5, BC064695.1, U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1. On Jun 2, 2019 this sequence version replaced NM_053026.3. Transcript Variant: This variant (2) does not utilize exon 11, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF069601.2 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAGTCAG TGGCCTTTCC CCCTTCATGG GAGACAACGA TAACGAAACC
    TTGGCCAACG TTACCTCAGC CACCTGGGAC TTCGACGACG AGGCATTCGA TGAGATCTCC
    GACGATGCCA AGGATTTCAT CAGCAATCTG CTGAAGAAAG ATATGAAAAA CCGCCTGGAC
    TGCACGCAGT GCCTTCAGCA TCCATGGCTA ATGAAAGATA CCAAGAACAT GGAGGCCAAG
    AAACTCTCCA AGGACCGGAT GAAGAAGTAC ATGGCAAGAA GGAAATGGCA GAAAACGGGC
    AATGCTGTGA GAGCCATTGG AAGACTGTCC TCTATGGCAA TGATCTCAGG GCTCAGTGGC
    AGGAAATCCT CAACAGGGTC ACCAACCAGC CCGCTCAATG CAGAAAAACT AGAATCTGAA
    GAAGATGTGT CCCAAGCTTT CCTTGAGGCT GTTGCTGAGG AAAAGCCTCA TGTAAAACCC
    TATTTCTCTA AGACCATTCG CGATTTAGAA GTTGTGGAGG GAAGTGCTGC TAGATTTGAC
    TGCAAGATTG AAGGATACCC AGACCCCGAG GTTGTCTGGT TCAAAGATGA CCAGTCAATC
    AGGGAGTCCC GCCACTTCCA GATAGACTAC GATGAGGACG GGAACTGCTC TTTAATTATT
    AGTGATGTTT GCGGGGATGA CGATGCCAAG TACACCTGCA AGGCTGTCAA CAGTCTTGGA
    GAAGCCACCT GCACAGCAGA GCTCATTGTG GAAACGATGG AGGAAGGTGA AGGGGAAGGG
    GAAGAGGAAG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444254.3
    CDS313..5850
    Translation

    Target ORF information:

    RefSeq Version NM_053026.4
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053026.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAGTCAG TGGCCTTTCC CCCTTCATGG GAGACAACGA TAACGAAACC
    TTGGCCAACG TTACCTCAGC CACCTGGGAC TTCGACGACG AGGCATTCGA TGAGATCTCC
    GACGATGCCA AGGATTTCAT CAGCAATCTG CTGAAGAAAG ATATGAAAAA CCGCCTGGAC
    TGCACGCAGT GCCTTCAGCA TCCATGGCTA ATGAAAGATA CCAAGAACAT GGAGGCCAAG
    AAACTCTCCA AGGACCGGAT GAAGAAGTAC ATGGCAAGAA GGAAATGGCA GAAAACGGGC
    AATGCTGTGA GAGCCATTGG AAGACTGTCC TCTATGGCAA TGATCTCAGG GCTCAGTGGC
    AGGAAATCCT CAACAGGGTC ACCAACCAGC CCGCTCAATG CAGAAAAACT AGAATCTGAA
    GAAGATGTGT CCCAAGCTTT CCTTGAGGCT GTTGCTGAGG AAAAGCCTCA TGTAAAACCC
    TATTTCTCTA AGACCATTCG CGATTTAGAA GTTGTGGAGG GAAGTGCTGC TAGATTTGAC
    TGCAAGATTG AAGGATACCC AGACCCCGAG GTTGTCTGGT TCAAAGATGA CCAGTCAATC
    AGGGAGTCCC GCCACTTCCA GATAGACTAC GATGAGGACG GGAACTGCTC TTTAATTATT
    AGTGATGTTT GCGGGGATGA CGATGCCAAG TACACCTGCA AGGCTGTCAA CAGTCTTGGA
    GAAGCCACCT GCACAGCAGA GCTCATTGTG GAAACGATGG AGGAAGGTGA AGGGGAAGGG
    GAAGAGGAAG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16318
    Clone ID Related Accession (Same CDS sequence) NM_053026.4 , NM_053026.3 , XM_024453535.1
    Accession Version NM_053026.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC064695.1, AC117381.5, U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1. On Oct 10, 2006 this sequence version replaced NM_053026.2. Transcript Variant: This variant (2) does not utilize exon 11, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF069601.2, AY424269.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAGTCAG TGGCCTTTCC CCCTTCATGG GAGACAACGA TAACGAAACC
    TTGGCCAACG TTACCTCAGC CACCTGGGAC TTCGACGACG AGGCATTCGA TGAGATCTCC
    GACGATGCCA AGGATTTCAT CAGCAATCTG CTGAAGAAAG ATATGAAAAA CCGCCTGGAC
    TGCACGCAGT GCCTTCAGCA TCCATGGCTA ATGAAAGATA CCAAGAACAT GGAGGCCAAG
    AAACTCTCCA AGGACCGGAT GAAGAAGTAC ATGGCAAGAA GGAAATGGCA GAAAACGGGC
    AATGCTGTGA GAGCCATTGG AAGACTGTCC TCTATGGCAA TGATCTCAGG GCTCAGTGGC
    AGGAAATCCT CAACAGGGTC ACCAACCAGC CCGCTCAATG CAGAAAAACT AGAATCTGAA
    GAAGATGTGT CCCAAGCTTT CCTTGAGGCT GTTGCTGAGG AAAAGCCTCA TGTAAAACCC
    TATTTCTCTA AGACCATTCG CGATTTAGAA GTTGTGGAGG GAAGTGCTGC TAGATTTGAC
    TGCAAGATTG AAGGATACCC AGACCCCGAG GTTGTCTGGT TCAAAGATGA CCAGTCAATC
    AGGGAGTCCC GCCACTTCCA GATAGACTAC GATGAGGACG GGAACTGCTC TTTAATTATT
    AGTGATGTTT GCGGGGATGA CGATGCCAAG TACACCTGCA AGGCTGTCAA CAGTCTTGGA
    GAAGCCACCT GCACAGCAGA GCTCATTGTG GAAACGATGG AGGAAGGTGA AGGGGAAGGG
    GAAGAGGAAG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444254.3
    CDS283..5820
    Translation

    Target ORF information:

    RefSeq Version NM_053026.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053026.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAGTCAG TGGCCTTTCC CCCTTCATGG GAGACAACGA TAACGAAACC
    TTGGCCAACG TTACCTCAGC CACCTGGGAC TTCGACGACG AGGCATTCGA TGAGATCTCC
    GACGATGCCA AGGATTTCAT CAGCAATCTG CTGAAGAAAG ATATGAAAAA CCGCCTGGAC
    TGCACGCAGT GCCTTCAGCA TCCATGGCTA ATGAAAGATA CCAAGAACAT GGAGGCCAAG
    AAACTCTCCA AGGACCGGAT GAAGAAGTAC ATGGCAAGAA GGAAATGGCA GAAAACGGGC
    AATGCTGTGA GAGCCATTGG AAGACTGTCC TCTATGGCAA TGATCTCAGG GCTCAGTGGC
    AGGAAATCCT CAACAGGGTC ACCAACCAGC CCGCTCAATG CAGAAAAACT AGAATCTGAA
    GAAGATGTGT CCCAAGCTTT CCTTGAGGCT GTTGCTGAGG AAAAGCCTCA TGTAAAACCC
    TATTTCTCTA AGACCATTCG CGATTTAGAA GTTGTGGAGG GAAGTGCTGC TAGATTTGAC
    TGCAAGATTG AAGGATACCC AGACCCCGAG GTTGTCTGGT TCAAAGATGA CCAGTCAATC
    AGGGAGTCCC GCCACTTCCA GATAGACTAC GATGAGGACG GGAACTGCTC TTTAATTATT
    AGTGATGTTT GCGGGGATGA CGATGCCAAG TACACCTGCA AGGCTGTCAA CAGTCTTGGA
    GAAGCCACCT GCACAGCAGA GCTCATTGTG GAAACGATGG AGGAAGGTGA AGGGGAAGGG
    GAAGAGGAAG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16325
    Clone ID Related Accession (Same CDS sequence) NM_053028.3 , NM_053028.4
    Accession Version NM_053028.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 3B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC064695.1, AC117381.5, U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1. On Oct 10, 2006 this sequence version replaced NM_053028.2. Transcript Variant: This variant (3B) does not utilize exons 11 and 30, compared to variant 1, resulting in a shorter protein (isoform 3B), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF069603.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444256.3
    CDS283..5667
    Translation

    Target ORF information:

    RefSeq Version NM_053028.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 3B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053028.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16325
    Clone ID Related Accession (Same CDS sequence) NM_053028.3 , NM_053028.4
    Accession Version NM_053028.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 3B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117381.5, BC064695.1, U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1. On Jun 2, 2019 this sequence version replaced NM_053028.3. Transcript Variant: This variant (3B) does not utilize exons 11 and 30, compared to variant 1, resulting in a shorter protein (isoform 3B), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF069603.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444256.3
    CDS313..5697
    Translation

    Target ORF information:

    RefSeq Version NM_053028.4
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 3B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053028.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17800
    Clone ID Related Accession (Same CDS sequence) NM_053025.4 , NM_053025.3 , XM_024453536.1 , XM_024453537.1 , XM_024453533.1
    Accession Version NM_053025.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117381.5, BC064695.1, U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1. On Jun 2, 2019 this sequence version replaced NM_053025.3. Transcript Variant: This variant (1) is the full-length transcript and encodes the full-length nonmuscle isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U48959.2 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444253.3
    CDS313..6057
    Translation

    Target ORF information:

    RefSeq Version NM_053025.4
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053025.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17800
    Clone ID Related Accession (Same CDS sequence) NM_053025.4 , NM_053025.3 , XM_024453536.1 , XM_024453537.1 , XM_024453533.1
    Accession Version NM_053025.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC064695.1, AC117381.5, U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1. This sequence is a reference standard in the RefSeqGene project. On Oct 10, 2006 this sequence version replaced NM_053025.2. Transcript Variant: This variant (1) is the full-length transcript and encodes the full-length nonmuscle isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U48959.2, AY424270.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_444253.3
    CDS283..6027
    Translation

    Target ORF information:

    RefSeq Version NM_053025.3
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053025.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59147
    Clone ID Related Accession (Same CDS sequence) NM_001321309.1 , NM_001321309.2
    Accession Version NM_001321309.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 9
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC064695.1, AC117381.5, DA853780.1, U48959.2, AC020634.16, AY339601.1, BC113458.1 and BQ006428.1. On Mar 22, 2016 this sequence version replaced XM_011512862.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGGACGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAAATGTTC CACTGCAGCC GAGTGCCCGT GTGTCTGTGT CTGAGAAGAA CGGCATGCAG
    GTTCTGGAAA TCCATGGAGT CAACCAAGAT GACGTGGGAG TGTACACGTG CCTGGTGGTG
    AACGGGTCGG GGAAGGCCTC GATGTCAGCT GAACTTTCCA TCCAAGGTTT GGACAGTGCC
    AATAGGTCAT TTGTGAGAGA AACAAAAGCC ACCAATTCAG ATGTCAGGAA AGAGGTGACC
    AATGTAATCT CAAAGGAGTC GAAGCTGGAC AGTCTGGAGG CTGCAGCCAA AAGCAAGAAC
    TGCTCCAGCC CCCAGAGAGG TGGCTCCCCA CCCTGGGCTG CAAACAGCCA GCCTCAGCCC
    CCAAGGGAGT CCAAGCTGGA GTCATGCAAG GACTCGCCCA GAACGGCCCC GCAGACCCCG
    GTCCTTCAGA AGACTTCCAG CTCCATCACC CTGCAGGCCG CAAGAGTTCA GCCGGAACCA
    AGAGCACCAG GCCTGGGGGT CCTATCACCT TCTGGAGAAG AGAGGAAGAG GCCAGCTCCT
    CCCCGTCCAG CCACCTTCCC CACCAGGCAG CCTGGCCTGG GGAGCCAAGA TGTTGTGAGC
    AAGGCTGCTA ACAGGAGAAT CCCCATGGAG GGCCAGAGGG ATTCAGCATT CCCCAAATTT
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAAG GAAAATCAAA CTGTCAAGTT CAGATGTGAA
    GTTTCCGGGA TTCCAAAGCC TGAAGTGGCC TGGTTCCTGG AAGGCACCCC CGTGAGGAGA
    CAGGAAGGCA GCATTGAGGT TTATGAAGAT GCTGGCTCCC ATTACCTCTG CCTGCTGAAA
    GCCCGGACCA GGGACAGTGG GACATACAGC TGCACTGCTT CCAACGCCCA AGGCCAGCTG
    TCCTGTAGCT GGACCCTCCA AGTGGAAAGG CTTGCCGTGA TGGAGGTGGC CCCCTCCTTC
    TCCAGTGTCC TGAAGGACTG CGCTGTTATT GAGGGCCAGG ATTTTGTGCT GCAGTGCTCC
    GTACGGGGGA CCCCAGTGCC CCGGATCACT TGGCTGCTGA ATGGGCAGCC CATCCAGTAC
    GCTCGCTCCA CCTGCGAGGC CGGCGTGGCT GAGCTCCACA TCCAGGATGC CCTGCCGGAG
    GACCATGGCA CCTACACCTG CCTAGCTGAG AATGCCTTGG GGCAGGTGTC CTGCAGCGCC
    TGGGTCACCG TCCATGAAAA GAAGAGTAGC AGGAAGAGTG AGTACCTTCT GCCTGTGGCT
    CCCAGCAAGC CCACTGCACC CATCTTCCTG CAGGGCCTCT CTGATCTCAA AGTCATGGAT
    GGAAGCCAGG TCACTATGAC TGTCCAAGTG TCAGGGAATC CACCCCCTGA AGTCATCTGG
    CTGCACAATG GGAATGAGAT CCAAGAGTCA GAGGACTTCC ACTTTGAACA GAGAGGAACT
    CAGCACAGCC TTTGTATCCA GGAAGTGTTC CCGGAGGACA CGGGCACGTA CACCTGCGAG
    GCCTGGAACA GCGCTGGAGA GGTCCGCACC CAGGCCGTGC TCACGGTACA AGAGCCTCAC
    GATGGCACCC AGCCCTGGTT CATCAGTAAG CCTCGCTCAG TGACAGCCTC CCTGGGCCAG
    AGTGTCCTCA TCTCCTGCGC CATAGCTGGT GACCCCTTTC CTACCGTGCA CTGGCTCAGA
    GATGGCAAAG CCCTCTGCAA AGACACTGGC CACTTCGAGG TGCTTCAGAA TGAGGACGTG
    TTCACCCTGG TTCTAAAGAA GGTGCAGCCC TGGCATGCCG GCCAGTATGA GATCCTGCTC
    AAGAACCGGG TTGGCGAATG CAGTTGCCAG GTGTCACTGA TGCTACAGAA CAGCTCTGCC
    AGAGCCCTTC CACGGGGGAG GGAGCCTGCC AGCTGCGAGG ACCTCTGTGG TGGAGGAGTT
    GGTGCTGATG GTGGTGGTAG TGACCGCTAT GGGTCCCTGA GGCCTGGCTG GCCAGCAAGA
    GGGCAGGGTT GGCTAGAGGA GGAAGACGGC GAGGACGTGC GAGGGGTGCT GAAGAGGCGC
    GTGGAGACGA GGCAGCACAC TGAGGAGGCG ATCCGCCAGC AGGAGGTGGA GCAGCTGGAC
    TTCCGAGACC TCCTGGGGAA GAAGGTGAGT ACAAAGACCC TATCGGAAGA CGACCTGAAG
    GAGATCCCAG CCGAGCAGAT GGATTTCCGT GCCAACCTGC AGCGGCAAGT GAAGCCAAAG
    ACTGTGTCTG AGGAAGAGAG GAAGGTGCAC AGCCCCCAGC AGGTCGATTT TCGCTCTGTC
    CTGGCCAAGA AGGGGACTTC CAAGACCCCC GTGCCTGAGA AGGTGCCACC GCCAAAACCT
    GCCACCCCGG ATTTTCGCTC AGTGCTGGGT GGCAAGAAGA AATTACCAGC AGAGAATGGC
    AGCAGCAGTG CCGAGACCCT GAATGCCAAG GCAGTGGAGA GTTCCAAGCC CCTGAGCAAT
    GCACAGCCTT CAGGGCCCTT GAAACCCGTG GGCAACGCCA AGCCTGCTGA GACCCTGAAG
    CCAATGGGCA ACGCCAAGCC TGCCGAGACC CTGAAGCCCA TGGGCAATGC CAAGCCTGAT
    GAGAACCTGA AATCCGCTAG CAAAGAAGAA CTCAAGAAAG ACGTTAAGAA TGATGTGAAC
    TGCAAGAGAG GCCATGCAGG GACCACAGAT AATGAAAAGA GATCAGAGAG CCAGGGGACA
    GCCCCAGCCT TCAAGCAGAA GCTGCAAGAT GTTCATGTGG CAGAGGGCAA GAAGCTGCTG
    CTCCAGTGCC AGGTGTCTTC TGACCCCCCA GCCACCATCA TCTGGACGCT GAACGGAAAG
    ACCCTCAAGA CCACCAAGTT CATCATCCTC TCCCAGGAAG GCTCACTCTG CTCCGTCTCC
    ATCGAGAAGG CACTGCCTGA GGACAGAGGC TTATACAAGT GTGTAGCCAA GAATGACGCT
    GGCCAGGCGG AGTGCTCCTG CCAAGTCACC GTGGATGATG CTCCAGCCAG TGAGAACACC
    AAGGCCCCAG AGATGAAATC CCGGAGGCCC AAGAGCTCTC TTCCTCCCGT GCTAGGAACT
    GAGAGTGATG CGACTGTGAA AAAGAAACCT GCCCCCAAGA CACCTCCGAA GGCAGCAATG
    CCCCCTCAGA TCATCCAGTT CCCTGAGGAC CAGAAGGTAC GCGCAGGAGA GTCAGTGGAG
    CTGTTTGGCA AAGTGACAGG CACTCAGCCC ATCACCTGTA CCTGGATGAA GTTCCGAAAG
    CAGATCCAGG AAAGCGAGCA CATGAAGGTG GAGAACAGCG AGAATGGCAG CAAGCTCACC
    ATCCTGGCCG CGCGCCAGGA GCACTGCGGC TGCTACACAC TGCTGGTGGA GAACAAGCTG
    GGCAGCAGGC AGGCCCAGGT CAACCTCACT GTCGTGGATA AGCCAGACCC CCCAGCTGGC
    ACACCTTGTG CCTCTGACAT TCGGAGCTCC TCACTGACCC TGTCCTGGTA TGGCTCCTCA
    TATGATGGGG GCAGTGCTGT ACAGTCCTAC AGCATCGAGA TCTGGGACTC AGCCAACAAG
    ACGTGGAAGG AACTAGCCAC ATGCCGCAGC ACCTCTTTCA ACGTCCAGGA CCTGCTGCCT
    GACCACGAAT ATAAGTTCCG TGTACGTGCA ATCAACGTGT ATGGAACCAG TGAGCCAAGC
    CAGGAGTCTG AACTCACAAC GGTAGGAGAG AAACCTGAAG AGCCGAAGGA TGAAGTGGAG
    GTGTCAGATG ATGATGAGAA GGAGCCCGAG GTTGATTACC GGACAGTGAC AATCAATACT
    GAACAAAAAG TATCTGACTT CTACGACATT GAGGAGAGAT TAGGATCTGG GAAATTTGGA
    CAGGTCTTTC GACTTGTAGA AAAGAAAACT CGAAAAGTCT GGGCAGGGAA GTTCTTCAAG
    GCATATTCAG CAAAAGAGAA AGAGAATATC CGGCAGGAGA TTAGCATCAT GAACTGCCTC
    CACCACCCTA AGCTGGTCCA GTGTGTGGAT GCCTTTGAAG AAAAGGCCAA CATCGTCATG
    GTCCTGGAGA TCGTGTCAGG AGGGGAGCTG TTTGAGCGCA TCATTGACGA GGACTTTGAG
    CTGACGGAGC GTGAGTGCAT CAAGTACATG CGGCAGATCT CGGAGGGAGT GGAGTACATC
    CACAAGCAGG GCATCGTGCA CCTGGACCTC AAGCCGGAGA ACATCATGTG TGTCAACAAG
    ACGGGCACCA GGATCAAGCT CATCGACTTT GGTCTGGCCA GGAGGCTGGA GAATGCGGGG
    TCTCTGAAGG TCCTCTTTGG CACCCCAGAA TTTGTGGCTC CTGAAGTGAT CAACTATGAG
    CCCATCGGCT ACGCCACAGA CATGTGGAGC ATCGGGGTCA TCTGCTACAT CCTAGTCAGT
    GGCCTTTCCC CCTTCATGGG AGACAACGAT AACGAAACCT TGGCCAACGT TACCTCAGCC
    ACCTGGGACT TCGACGACGA GGCATTCGAT GAGATCTCCG ACGATGCCAA GGATTTCATC
    AGCAATCTGC TGAAGAAAGA TATGAAAAAC CGCCTGGACT GCACGCAGTG CCTTCAGCAT
    CCATGGCTAA TGAAAGATAC CAAGAACATG GAGGCCAAGA AACTCTCCAA GGACCGGATG
    AAGAAGTACA TGGCAAGAAG GAAATGGCAG AAAACGGGCA ATGCTGTGAG AGCCATTGGA
    AGACTGTCCT CTATGGCAAT GATCTCAGGG CTCAGTGGCA GGAAATCCTC AACAGGGTCA
    CCAACCAGCC CGCTCAATGC AGAAAAACTA GAATCTGAAG AAGATGTGTC CCAAGCTTTC
    CTTGAGGCTG TTGCTGAGGA AAAGCCTCAT GTAAAACCCT ATTTCTCTAA GACCATTCGC
    GATTTAGAAG TTGTGGAGGG AAGTGCTGCT AGATTTGACT GCAAGATTGA AGGATACCCA
    GACCCCGAGG TTGTCTGGTT CAAAGATGAC CAGTCAATCA GGGAGTCCCG CCACTTCCAG
    ATAGACTACG ATGAGGACGG GAACTGCTCT TTAATTATTA GTGATGTTTG CGGGGATGAC
    GATGCCAAGT ACACCTGCAA GGCTGTCAAC AGTCTTGGAG AAGCCACCTG CACAGCAGAG
    CTCATTGTGG AAACGATGGA GGAAGGTGAA GGGGAAGGGG AAGAGGAAGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308238.1
    CDS603..5819
    Translation

    Target ORF information:

    RefSeq Version NM_001321309.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGGACGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAAATGTTC CACTGCAGCC GAGTGCCCGT GTGTCTGTGT CTGAGAAGAA CGGCATGCAG
    GTTCTGGAAA TCCATGGAGT CAACCAAGAT GACGTGGGAG TGTACACGTG CCTGGTGGTG
    AACGGGTCGG GGAAGGCCTC GATGTCAGCT GAACTTTCCA TCCAAGGTTT GGACAGTGCC
    AATAGGTCAT TTGTGAGAGA AACAAAAGCC ACCAATTCAG ATGTCAGGAA AGAGGTGACC
    AATGTAATCT CAAAGGAGTC GAAGCTGGAC AGTCTGGAGG CTGCAGCCAA AAGCAAGAAC
    TGCTCCAGCC CCCAGAGAGG TGGCTCCCCA CCCTGGGCTG CAAACAGCCA GCCTCAGCCC
    CCAAGGGAGT CCAAGCTGGA GTCATGCAAG GACTCGCCCA GAACGGCCCC GCAGACCCCG
    GTCCTTCAGA AGACTTCCAG CTCCATCACC CTGCAGGCCG CAAGAGTTCA GCCGGAACCA
    AGAGCACCAG GCCTGGGGGT CCTATCACCT TCTGGAGAAG AGAGGAAGAG GCCAGCTCCT
    CCCCGTCCAG CCACCTTCCC CACCAGGCAG CCTGGCCTGG GGAGCCAAGA TGTTGTGAGC
    AAGGCTGCTA ACAGGAGAAT CCCCATGGAG GGCCAGAGGG ATTCAGCATT CCCCAAATTT
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAAG GAAAATCAAA CTGTCAAGTT CAGATGTGAA
    GTTTCCGGGA TTCCAAAGCC TGAAGTGGCC TGGTTCCTGG AAGGCACCCC CGTGAGGAGA
    CAGGAAGGCA GCATTGAGGT TTATGAAGAT GCTGGCTCCC ATTACCTCTG CCTGCTGAAA
    GCCCGGACCA GGGACAGTGG GACATACAGC TGCACTGCTT CCAACGCCCA AGGCCAGCTG
    TCCTGTAGCT GGACCCTCCA AGTGGAAAGG CTTGCCGTGA TGGAGGTGGC CCCCTCCTTC
    TCCAGTGTCC TGAAGGACTG CGCTGTTATT GAGGGCCAGG ATTTTGTGCT GCAGTGCTCC
    GTACGGGGGA CCCCAGTGCC CCGGATCACT TGGCTGCTGA ATGGGCAGCC CATCCAGTAC
    GCTCGCTCCA CCTGCGAGGC CGGCGTGGCT GAGCTCCACA TCCAGGATGC CCTGCCGGAG
    GACCATGGCA CCTACACCTG CCTAGCTGAG AATGCCTTGG GGCAGGTGTC CTGCAGCGCC
    TGGGTCACCG TCCATGAAAA GAAGAGTAGC AGGAAGAGTG AGTACCTTCT GCCTGTGGCT
    CCCAGCAAGC CCACTGCACC CATCTTCCTG CAGGGCCTCT CTGATCTCAA AGTCATGGAT
    GGAAGCCAGG TCACTATGAC TGTCCAAGTG TCAGGGAATC CACCCCCTGA AGTCATCTGG
    CTGCACAATG GGAATGAGAT CCAAGAGTCA GAGGACTTCC ACTTTGAACA GAGAGGAACT
    CAGCACAGCC TTTGTATCCA GGAAGTGTTC CCGGAGGACA CGGGCACGTA CACCTGCGAG
    GCCTGGAACA GCGCTGGAGA GGTCCGCACC CAGGCCGTGC TCACGGTACA AGAGCCTCAC
    GATGGCACCC AGCCCTGGTT CATCAGTAAG CCTCGCTCAG TGACAGCCTC CCTGGGCCAG
    AGTGTCCTCA TCTCCTGCGC CATAGCTGGT GACCCCTTTC CTACCGTGCA CTGGCTCAGA
    GATGGCAAAG CCCTCTGCAA AGACACTGGC CACTTCGAGG TGCTTCAGAA TGAGGACGTG
    TTCACCCTGG TTCTAAAGAA GGTGCAGCCC TGGCATGCCG GCCAGTATGA GATCCTGCTC
    AAGAACCGGG TTGGCGAATG CAGTTGCCAG GTGTCACTGA TGCTACAGAA CAGCTCTGCC
    AGAGCCCTTC CACGGGGGAG GGAGCCTGCC AGCTGCGAGG ACCTCTGTGG TGGAGGAGTT
    GGTGCTGATG GTGGTGGTAG TGACCGCTAT GGGTCCCTGA GGCCTGGCTG GCCAGCAAGA
    GGGCAGGGTT GGCTAGAGGA GGAAGACGGC GAGGACGTGC GAGGGGTGCT GAAGAGGCGC
    GTGGAGACGA GGCAGCACAC TGAGGAGGCG ATCCGCCAGC AGGAGGTGGA GCAGCTGGAC
    TTCCGAGACC TCCTGGGGAA GAAGGTGAGT ACAAAGACCC TATCGGAAGA CGACCTGAAG
    GAGATCCCAG CCGAGCAGAT GGATTTCCGT GCCAACCTGC AGCGGCAAGT GAAGCCAAAG
    ACTGTGTCTG AGGAAGAGAG GAAGGTGCAC AGCCCCCAGC AGGTCGATTT TCGCTCTGTC
    CTGGCCAAGA AGGGGACTTC CAAGACCCCC GTGCCTGAGA AGGTGCCACC GCCAAAACCT
    GCCACCCCGG ATTTTCGCTC AGTGCTGGGT GGCAAGAAGA AATTACCAGC AGAGAATGGC
    AGCAGCAGTG CCGAGACCCT GAATGCCAAG GCAGTGGAGA GTTCCAAGCC CCTGAGCAAT
    GCACAGCCTT CAGGGCCCTT GAAACCCGTG GGCAACGCCA AGCCTGCTGA GACCCTGAAG
    CCAATGGGCA ACGCCAAGCC TGCCGAGACC CTGAAGCCCA TGGGCAATGC CAAGCCTGAT
    GAGAACCTGA AATCCGCTAG CAAAGAAGAA CTCAAGAAAG ACGTTAAGAA TGATGTGAAC
    TGCAAGAGAG GCCATGCAGG GACCACAGAT AATGAAAAGA GATCAGAGAG CCAGGGGACA
    GCCCCAGCCT TCAAGCAGAA GCTGCAAGAT GTTCATGTGG CAGAGGGCAA GAAGCTGCTG
    CTCCAGTGCC AGGTGTCTTC TGACCCCCCA GCCACCATCA TCTGGACGCT GAACGGAAAG
    ACCCTCAAGA CCACCAAGTT CATCATCCTC TCCCAGGAAG GCTCACTCTG CTCCGTCTCC
    ATCGAGAAGG CACTGCCTGA GGACAGAGGC TTATACAAGT GTGTAGCCAA GAATGACGCT
    GGCCAGGCGG AGTGCTCCTG CCAAGTCACC GTGGATGATG CTCCAGCCAG TGAGAACACC
    AAGGCCCCAG AGATGAAATC CCGGAGGCCC AAGAGCTCTC TTCCTCCCGT GCTAGGAACT
    GAGAGTGATG CGACTGTGAA AAAGAAACCT GCCCCCAAGA CACCTCCGAA GGCAGCAATG
    CCCCCTCAGA TCATCCAGTT CCCTGAGGAC CAGAAGGTAC GCGCAGGAGA GTCAGTGGAG
    CTGTTTGGCA AAGTGACAGG CACTCAGCCC ATCACCTGTA CCTGGATGAA GTTCCGAAAG
    CAGATCCAGG AAAGCGAGCA CATGAAGGTG GAGAACAGCG AGAATGGCAG CAAGCTCACC
    ATCCTGGCCG CGCGCCAGGA GCACTGCGGC TGCTACACAC TGCTGGTGGA GAACAAGCTG
    GGCAGCAGGC AGGCCCAGGT CAACCTCACT GTCGTGGATA AGCCAGACCC CCCAGCTGGC
    ACACCTTGTG CCTCTGACAT TCGGAGCTCC TCACTGACCC TGTCCTGGTA TGGCTCCTCA
    TATGATGGGG GCAGTGCTGT ACAGTCCTAC AGCATCGAGA TCTGGGACTC AGCCAACAAG
    ACGTGGAAGG AACTAGCCAC ATGCCGCAGC ACCTCTTTCA ACGTCCAGGA CCTGCTGCCT
    GACCACGAAT ATAAGTTCCG TGTACGTGCA ATCAACGTGT ATGGAACCAG TGAGCCAAGC
    CAGGAGTCTG AACTCACAAC GGTAGGAGAG AAACCTGAAG AGCCGAAGGA TGAAGTGGAG
    GTGTCAGATG ATGATGAGAA GGAGCCCGAG GTTGATTACC GGACAGTGAC AATCAATACT
    GAACAAAAAG TATCTGACTT CTACGACATT GAGGAGAGAT TAGGATCTGG GAAATTTGGA
    CAGGTCTTTC GACTTGTAGA AAAGAAAACT CGAAAAGTCT GGGCAGGGAA GTTCTTCAAG
    GCATATTCAG CAAAAGAGAA AGAGAATATC CGGCAGGAGA TTAGCATCAT GAACTGCCTC
    CACCACCCTA AGCTGGTCCA GTGTGTGGAT GCCTTTGAAG AAAAGGCCAA CATCGTCATG
    GTCCTGGAGA TCGTGTCAGG AGGGGAGCTG TTTGAGCGCA TCATTGACGA GGACTTTGAG
    CTGACGGAGC GTGAGTGCAT CAAGTACATG CGGCAGATCT CGGAGGGAGT GGAGTACATC
    CACAAGCAGG GCATCGTGCA CCTGGACCTC AAGCCGGAGA ACATCATGTG TGTCAACAAG
    ACGGGCACCA GGATCAAGCT CATCGACTTT GGTCTGGCCA GGAGGCTGGA GAATGCGGGG
    TCTCTGAAGG TCCTCTTTGG CACCCCAGAA TTTGTGGCTC CTGAAGTGAT CAACTATGAG
    CCCATCGGCT ACGCCACAGA CATGTGGAGC ATCGGGGTCA TCTGCTACAT CCTAGTCAGT
    GGCCTTTCCC CCTTCATGGG AGACAACGAT AACGAAACCT TGGCCAACGT TACCTCAGCC
    ACCTGGGACT TCGACGACGA GGCATTCGAT GAGATCTCCG ACGATGCCAA GGATTTCATC
    AGCAATCTGC TGAAGAAAGA TATGAAAAAC CGCCTGGACT GCACGCAGTG CCTTCAGCAT
    CCATGGCTAA TGAAAGATAC CAAGAACATG GAGGCCAAGA AACTCTCCAA GGACCGGATG
    AAGAAGTACA TGGCAAGAAG GAAATGGCAG AAAACGGGCA ATGCTGTGAG AGCCATTGGA
    AGACTGTCCT CTATGGCAAT GATCTCAGGG CTCAGTGGCA GGAAATCCTC AACAGGGTCA
    CCAACCAGCC CGCTCAATGC AGAAAAACTA GAATCTGAAG AAGATGTGTC CCAAGCTTTC
    CTTGAGGCTG TTGCTGAGGA AAAGCCTCAT GTAAAACCCT ATTTCTCTAA GACCATTCGC
    GATTTAGAAG TTGTGGAGGG AAGTGCTGCT AGATTTGACT GCAAGATTGA AGGATACCCA
    GACCCCGAGG TTGTCTGGTT CAAAGATGAC CAGTCAATCA GGGAGTCCCG CCACTTCCAG
    ATAGACTACG ATGAGGACGG GAACTGCTCT TTAATTATTA GTGATGTTTG CGGGGATGAC
    GATGCCAAGT ACACCTGCAA GGCTGTCAAC AGTCTTGGAG AAGCCACCTG CACAGCAGAG
    CTCATTGTGG AAACGATGGA GGAAGGTGAA GGGGAAGGGG AAGAGGAAGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59147
    Clone ID Related Accession (Same CDS sequence) NM_001321309.1 , NM_001321309.2
    Accession Version NM_001321309.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform 9
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117381.5, BC064695.1, DA853780.1, U48959.2, AC020634.16, AY339601.1, BC113458.1, BQ006428.1 and AC023165.20. On Jun 2, 2019 this sequence version replaced NM_001321309.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGGACGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAAATGTTC CACTGCAGCC GAGTGCCCGT GTGTCTGTGT CTGAGAAGAA CGGCATGCAG
    GTTCTGGAAA TCCATGGAGT CAACCAAGAT GACGTGGGAG TGTACACGTG CCTGGTGGTG
    AACGGGTCGG GGAAGGCCTC GATGTCAGCT GAACTTTCCA TCCAAGGTTT GGACAGTGCC
    AATAGGTCAT TTGTGAGAGA AACAAAAGCC ACCAATTCAG ATGTCAGGAA AGAGGTGACC
    AATGTAATCT CAAAGGAGTC GAAGCTGGAC AGTCTGGAGG CTGCAGCCAA AAGCAAGAAC
    TGCTCCAGCC CCCAGAGAGG TGGCTCCCCA CCCTGGGCTG CAAACAGCCA GCCTCAGCCC
    CCAAGGGAGT CCAAGCTGGA GTCATGCAAG GACTCGCCCA GAACGGCCCC GCAGACCCCG
    GTCCTTCAGA AGACTTCCAG CTCCATCACC CTGCAGGCCG CAAGAGTTCA GCCGGAACCA
    AGAGCACCAG GCCTGGGGGT CCTATCACCT TCTGGAGAAG AGAGGAAGAG GCCAGCTCCT
    CCCCGTCCAG CCACCTTCCC CACCAGGCAG CCTGGCCTGG GGAGCCAAGA TGTTGTGAGC
    AAGGCTGCTA ACAGGAGAAT CCCCATGGAG GGCCAGAGGG ATTCAGCATT CCCCAAATTT
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAAG GAAAATCAAA CTGTCAAGTT CAGATGTGAA
    GTTTCCGGGA TTCCAAAGCC TGAAGTGGCC TGGTTCCTGG AAGGCACCCC CGTGAGGAGA
    CAGGAAGGCA GCATTGAGGT TTATGAAGAT GCTGGCTCCC ATTACCTCTG CCTGCTGAAA
    GCCCGGACCA GGGACAGTGG GACATACAGC TGCACTGCTT CCAACGCCCA AGGCCAGCTG
    TCCTGTAGCT GGACCCTCCA AGTGGAAAGG CTTGCCGTGA TGGAGGTGGC CCCCTCCTTC
    TCCAGTGTCC TGAAGGACTG CGCTGTTATT GAGGGCCAGG ATTTTGTGCT GCAGTGCTCC
    GTACGGGGGA CCCCAGTGCC CCGGATCACT TGGCTGCTGA ATGGGCAGCC CATCCAGTAC
    GCTCGCTCCA CCTGCGAGGC CGGCGTGGCT GAGCTCCACA TCCAGGATGC CCTGCCGGAG
    GACCATGGCA CCTACACCTG CCTAGCTGAG AATGCCTTGG GGCAGGTGTC CTGCAGCGCC
    TGGGTCACCG TCCATGAAAA GAAGAGTAGC AGGAAGAGTG AGTACCTTCT GCCTGTGGCT
    CCCAGCAAGC CCACTGCACC CATCTTCCTG CAGGGCCTCT CTGATCTCAA AGTCATGGAT
    GGAAGCCAGG TCACTATGAC TGTCCAAGTG TCAGGGAATC CACCCCCTGA AGTCATCTGG
    CTGCACAATG GGAATGAGAT CCAAGAGTCA GAGGACTTCC ACTTTGAACA GAGAGGAACT
    CAGCACAGCC TTTGTATCCA GGAAGTGTTC CCGGAGGACA CGGGCACGTA CACCTGCGAG
    GCCTGGAACA GCGCTGGAGA GGTCCGCACC CAGGCCGTGC TCACGGTACA AGAGCCTCAC
    GATGGCACCC AGCCCTGGTT CATCAGTAAG CCTCGCTCAG TGACAGCCTC CCTGGGCCAG
    AGTGTCCTCA TCTCCTGCGC CATAGCTGGT GACCCCTTTC CTACCGTGCA CTGGCTCAGA
    GATGGCAAAG CCCTCTGCAA AGACACTGGC CACTTCGAGG TGCTTCAGAA TGAGGACGTG
    TTCACCCTGG TTCTAAAGAA GGTGCAGCCC TGGCATGCCG GCCAGTATGA GATCCTGCTC
    AAGAACCGGG TTGGCGAATG CAGTTGCCAG GTGTCACTGA TGCTACAGAA CAGCTCTGCC
    AGAGCCCTTC CACGGGGGAG GGAGCCTGCC AGCTGCGAGG ACCTCTGTGG TGGAGGAGTT
    GGTGCTGATG GTGGTGGTAG TGACCGCTAT GGGTCCCTGA GGCCTGGCTG GCCAGCAAGA
    GGGCAGGGTT GGCTAGAGGA GGAAGACGGC GAGGACGTGC GAGGGGTGCT GAAGAGGCGC
    GTGGAGACGA GGCAGCACAC TGAGGAGGCG ATCCGCCAGC AGGAGGTGGA GCAGCTGGAC
    TTCCGAGACC TCCTGGGGAA GAAGGTGAGT ACAAAGACCC TATCGGAAGA CGACCTGAAG
    GAGATCCCAG CCGAGCAGAT GGATTTCCGT GCCAACCTGC AGCGGCAAGT GAAGCCAAAG
    ACTGTGTCTG AGGAAGAGAG GAAGGTGCAC AGCCCCCAGC AGGTCGATTT TCGCTCTGTC
    CTGGCCAAGA AGGGGACTTC CAAGACCCCC GTGCCTGAGA AGGTGCCACC GCCAAAACCT
    GCCACCCCGG ATTTTCGCTC AGTGCTGGGT GGCAAGAAGA AATTACCAGC AGAGAATGGC
    AGCAGCAGTG CCGAGACCCT GAATGCCAAG GCAGTGGAGA GTTCCAAGCC CCTGAGCAAT
    GCACAGCCTT CAGGGCCCTT GAAACCCGTG GGCAACGCCA AGCCTGCTGA GACCCTGAAG
    CCAATGGGCA ACGCCAAGCC TGCCGAGACC CTGAAGCCCA TGGGCAATGC CAAGCCTGAT
    GAGAACCTGA AATCCGCTAG CAAAGAAGAA CTCAAGAAAG ACGTTAAGAA TGATGTGAAC
    TGCAAGAGAG GCCATGCAGG GACCACAGAT AATGAAAAGA GATCAGAGAG CCAGGGGACA
    GCCCCAGCCT TCAAGCAGAA GCTGCAAGAT GTTCATGTGG CAGAGGGCAA GAAGCTGCTG
    CTCCAGTGCC AGGTGTCTTC TGACCCCCCA GCCACCATCA TCTGGACGCT GAACGGAAAG
    ACCCTCAAGA CCACCAAGTT CATCATCCTC TCCCAGGAAG GCTCACTCTG CTCCGTCTCC
    ATCGAGAAGG CACTGCCTGA GGACAGAGGC TTATACAAGT GTGTAGCCAA GAATGACGCT
    GGCCAGGCGG AGTGCTCCTG CCAAGTCACC GTGGATGATG CTCCAGCCAG TGAGAACACC
    AAGGCCCCAG AGATGAAATC CCGGAGGCCC AAGAGCTCTC TTCCTCCCGT GCTAGGAACT
    GAGAGTGATG CGACTGTGAA AAAGAAACCT GCCCCCAAGA CACCTCCGAA GGCAGCAATG
    CCCCCTCAGA TCATCCAGTT CCCTGAGGAC CAGAAGGTAC GCGCAGGAGA GTCAGTGGAG
    CTGTTTGGCA AAGTGACAGG CACTCAGCCC ATCACCTGTA CCTGGATGAA GTTCCGAAAG
    CAGATCCAGG AAAGCGAGCA CATGAAGGTG GAGAACAGCG AGAATGGCAG CAAGCTCACC
    ATCCTGGCCG CGCGCCAGGA GCACTGCGGC TGCTACACAC TGCTGGTGGA GAACAAGCTG
    GGCAGCAGGC AGGCCCAGGT CAACCTCACT GTCGTGGATA AGCCAGACCC CCCAGCTGGC
    ACACCTTGTG CCTCTGACAT TCGGAGCTCC TCACTGACCC TGTCCTGGTA TGGCTCCTCA
    TATGATGGGG GCAGTGCTGT ACAGTCCTAC AGCATCGAGA TCTGGGACTC AGCCAACAAG
    ACGTGGAAGG AACTAGCCAC ATGCCGCAGC ACCTCTTTCA ACGTCCAGGA CCTGCTGCCT
    GACCACGAAT ATAAGTTCCG TGTACGTGCA ATCAACGTGT ATGGAACCAG TGAGCCAAGC
    CAGGAGTCTG AACTCACAAC GGTAGGAGAG AAACCTGAAG AGCCGAAGGA TGAAGTGGAG
    GTGTCAGATG ATGATGAGAA GGAGCCCGAG GTTGATTACC GGACAGTGAC AATCAATACT
    GAACAAAAAG TATCTGACTT CTACGACATT GAGGAGAGAT TAGGATCTGG GAAATTTGGA
    CAGGTCTTTC GACTTGTAGA AAAGAAAACT CGAAAAGTCT GGGCAGGGAA GTTCTTCAAG
    GCATATTCAG CAAAAGAGAA AGAGAATATC CGGCAGGAGA TTAGCATCAT GAACTGCCTC
    CACCACCCTA AGCTGGTCCA GTGTGTGGAT GCCTTTGAAG AAAAGGCCAA CATCGTCATG
    GTCCTGGAGA TCGTGTCAGG AGGGGAGCTG TTTGAGCGCA TCATTGACGA GGACTTTGAG
    CTGACGGAGC GTGAGTGCAT CAAGTACATG CGGCAGATCT CGGAGGGAGT GGAGTACATC
    CACAAGCAGG GCATCGTGCA CCTGGACCTC AAGCCGGAGA ACATCATGTG TGTCAACAAG
    ACGGGCACCA GGATCAAGCT CATCGACTTT GGTCTGGCCA GGAGGCTGGA GAATGCGGGG
    TCTCTGAAGG TCCTCTTTGG CACCCCAGAA TTTGTGGCTC CTGAAGTGAT CAACTATGAG
    CCCATCGGCT ACGCCACAGA CATGTGGAGC ATCGGGGTCA TCTGCTACAT CCTAGTCAGT
    GGCCTTTCCC CCTTCATGGG AGACAACGAT AACGAAACCT TGGCCAACGT TACCTCAGCC
    ACCTGGGACT TCGACGACGA GGCATTCGAT GAGATCTCCG ACGATGCCAA GGATTTCATC
    AGCAATCTGC TGAAGAAAGA TATGAAAAAC CGCCTGGACT GCACGCAGTG CCTTCAGCAT
    CCATGGCTAA TGAAAGATAC CAAGAACATG GAGGCCAAGA AACTCTCCAA GGACCGGATG
    AAGAAGTACA TGGCAAGAAG GAAATGGCAG AAAACGGGCA ATGCTGTGAG AGCCATTGGA
    AGACTGTCCT CTATGGCAAT GATCTCAGGG CTCAGTGGCA GGAAATCCTC AACAGGGTCA
    CCAACCAGCC CGCTCAATGC AGAAAAACTA GAATCTGAAG AAGATGTGTC CCAAGCTTTC
    CTTGAGGCTG TTGCTGAGGA AAAGCCTCAT GTAAAACCCT ATTTCTCTAA GACCATTCGC
    GATTTAGAAG TTGTGGAGGG AAGTGCTGCT AGATTTGACT GCAAGATTGA AGGATACCCA
    GACCCCGAGG TTGTCTGGTT CAAAGATGAC CAGTCAATCA GGGAGTCCCG CCACTTCCAG
    ATAGACTACG ATGAGGACGG GAACTGCTCT TTAATTATTA GTGATGTTTG CGGGGATGAC
    GATGCCAAGT ACACCTGCAA GGCTGTCAAC AGTCTTGGAG AAGCCACCTG CACAGCAGAG
    CTCATTGTGG AAACGATGGA GGAAGGTGAA GGGGAAGGGG AAGAGGAAGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308238.1
    CDS633..5849
    Translation

    Target ORF information:

    RefSeq Version NM_001321309.2
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321309.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGGACGAT TCTCCTGCAA GATCACTGGC CGGCCCCAAC CGCAGGTCAC CTGGCTCAAG 
    GGAAATGTTC CACTGCAGCC GAGTGCCCGT GTGTCTGTGT CTGAGAAGAA CGGCATGCAG
    GTTCTGGAAA TCCATGGAGT CAACCAAGAT GACGTGGGAG TGTACACGTG CCTGGTGGTG
    AACGGGTCGG GGAAGGCCTC GATGTCAGCT GAACTTTCCA TCCAAGGTTT GGACAGTGCC
    AATAGGTCAT TTGTGAGAGA AACAAAAGCC ACCAATTCAG ATGTCAGGAA AGAGGTGACC
    AATGTAATCT CAAAGGAGTC GAAGCTGGAC AGTCTGGAGG CTGCAGCCAA AAGCAAGAAC
    TGCTCCAGCC CCCAGAGAGG TGGCTCCCCA CCCTGGGCTG CAAACAGCCA GCCTCAGCCC
    CCAAGGGAGT CCAAGCTGGA GTCATGCAAG GACTCGCCCA GAACGGCCCC GCAGACCCCG
    GTCCTTCAGA AGACTTCCAG CTCCATCACC CTGCAGGCCG CAAGAGTTCA GCCGGAACCA
    AGAGCACCAG GCCTGGGGGT CCTATCACCT TCTGGAGAAG AGAGGAAGAG GCCAGCTCCT
    CCCCGTCCAG CCACCTTCCC CACCAGGCAG CCTGGCCTGG GGAGCCAAGA TGTTGTGAGC
    AAGGCTGCTA ACAGGAGAAT CCCCATGGAG GGCCAGAGGG ATTCAGCATT CCCCAAATTT
    GAGAGCAAGC CCCAAAGCCA GGAGGTCAAG GAAAATCAAA CTGTCAAGTT CAGATGTGAA
    GTTTCCGGGA TTCCAAAGCC TGAAGTGGCC TGGTTCCTGG AAGGCACCCC CGTGAGGAGA
    CAGGAAGGCA GCATTGAGGT TTATGAAGAT GCTGGCTCCC ATTACCTCTG CCTGCTGAAA
    GCCCGGACCA GGGACAGTGG GACATACAGC TGCACTGCTT CCAACGCCCA AGGCCAGCTG
    TCCTGTAGCT GGACCCTCCA AGTGGAAAGG CTTGCCGTGA TGGAGGTGGC CCCCTCCTTC
    TCCAGTGTCC TGAAGGACTG CGCTGTTATT GAGGGCCAGG ATTTTGTGCT GCAGTGCTCC
    GTACGGGGGA CCCCAGTGCC CCGGATCACT TGGCTGCTGA ATGGGCAGCC CATCCAGTAC
    GCTCGCTCCA CCTGCGAGGC CGGCGTGGCT GAGCTCCACA TCCAGGATGC CCTGCCGGAG
    GACCATGGCA CCTACACCTG CCTAGCTGAG AATGCCTTGG GGCAGGTGTC CTGCAGCGCC
    TGGGTCACCG TCCATGAAAA GAAGAGTAGC AGGAAGAGTG AGTACCTTCT GCCTGTGGCT
    CCCAGCAAGC CCACTGCACC CATCTTCCTG CAGGGCCTCT CTGATCTCAA AGTCATGGAT
    GGAAGCCAGG TCACTATGAC TGTCCAAGTG TCAGGGAATC CACCCCCTGA AGTCATCTGG
    CTGCACAATG GGAATGAGAT CCAAGAGTCA GAGGACTTCC ACTTTGAACA GAGAGGAACT
    CAGCACAGCC TTTGTATCCA GGAAGTGTTC CCGGAGGACA CGGGCACGTA CACCTGCGAG
    GCCTGGAACA GCGCTGGAGA GGTCCGCACC CAGGCCGTGC TCACGGTACA AGAGCCTCAC
    GATGGCACCC AGCCCTGGTT CATCAGTAAG CCTCGCTCAG TGACAGCCTC CCTGGGCCAG
    AGTGTCCTCA TCTCCTGCGC CATAGCTGGT GACCCCTTTC CTACCGTGCA CTGGCTCAGA
    GATGGCAAAG CCCTCTGCAA AGACACTGGC CACTTCGAGG TGCTTCAGAA TGAGGACGTG
    TTCACCCTGG TTCTAAAGAA GGTGCAGCCC TGGCATGCCG GCCAGTATGA GATCCTGCTC
    AAGAACCGGG TTGGCGAATG CAGTTGCCAG GTGTCACTGA TGCTACAGAA CAGCTCTGCC
    AGAGCCCTTC CACGGGGGAG GGAGCCTGCC AGCTGCGAGG ACCTCTGTGG TGGAGGAGTT
    GGTGCTGATG GTGGTGGTAG TGACCGCTAT GGGTCCCTGA GGCCTGGCTG GCCAGCAAGA
    GGGCAGGGTT GGCTAGAGGA GGAAGACGGC GAGGACGTGC GAGGGGTGCT GAAGAGGCGC
    GTGGAGACGA GGCAGCACAC TGAGGAGGCG ATCCGCCAGC AGGAGGTGGA GCAGCTGGAC
    TTCCGAGACC TCCTGGGGAA GAAGGTGAGT ACAAAGACCC TATCGGAAGA CGACCTGAAG
    GAGATCCCAG CCGAGCAGAT GGATTTCCGT GCCAACCTGC AGCGGCAAGT GAAGCCAAAG
    ACTGTGTCTG AGGAAGAGAG GAAGGTGCAC AGCCCCCAGC AGGTCGATTT TCGCTCTGTC
    CTGGCCAAGA AGGGGACTTC CAAGACCCCC GTGCCTGAGA AGGTGCCACC GCCAAAACCT
    GCCACCCCGG ATTTTCGCTC AGTGCTGGGT GGCAAGAAGA AATTACCAGC AGAGAATGGC
    AGCAGCAGTG CCGAGACCCT GAATGCCAAG GCAGTGGAGA GTTCCAAGCC CCTGAGCAAT
    GCACAGCCTT CAGGGCCCTT GAAACCCGTG GGCAACGCCA AGCCTGCTGA GACCCTGAAG
    CCAATGGGCA ACGCCAAGCC TGCCGAGACC CTGAAGCCCA TGGGCAATGC CAAGCCTGAT
    GAGAACCTGA AATCCGCTAG CAAAGAAGAA CTCAAGAAAG ACGTTAAGAA TGATGTGAAC
    TGCAAGAGAG GCCATGCAGG GACCACAGAT AATGAAAAGA GATCAGAGAG CCAGGGGACA
    GCCCCAGCCT TCAAGCAGAA GCTGCAAGAT GTTCATGTGG CAGAGGGCAA GAAGCTGCTG
    CTCCAGTGCC AGGTGTCTTC TGACCCCCCA GCCACCATCA TCTGGACGCT GAACGGAAAG
    ACCCTCAAGA CCACCAAGTT CATCATCCTC TCCCAGGAAG GCTCACTCTG CTCCGTCTCC
    ATCGAGAAGG CACTGCCTGA GGACAGAGGC TTATACAAGT GTGTAGCCAA GAATGACGCT
    GGCCAGGCGG AGTGCTCCTG CCAAGTCACC GTGGATGATG CTCCAGCCAG TGAGAACACC
    AAGGCCCCAG AGATGAAATC CCGGAGGCCC AAGAGCTCTC TTCCTCCCGT GCTAGGAACT
    GAGAGTGATG CGACTGTGAA AAAGAAACCT GCCCCCAAGA CACCTCCGAA GGCAGCAATG
    CCCCCTCAGA TCATCCAGTT CCCTGAGGAC CAGAAGGTAC GCGCAGGAGA GTCAGTGGAG
    CTGTTTGGCA AAGTGACAGG CACTCAGCCC ATCACCTGTA CCTGGATGAA GTTCCGAAAG
    CAGATCCAGG AAAGCGAGCA CATGAAGGTG GAGAACAGCG AGAATGGCAG CAAGCTCACC
    ATCCTGGCCG CGCGCCAGGA GCACTGCGGC TGCTACACAC TGCTGGTGGA GAACAAGCTG
    GGCAGCAGGC AGGCCCAGGT CAACCTCACT GTCGTGGATA AGCCAGACCC CCCAGCTGGC
    ACACCTTGTG CCTCTGACAT TCGGAGCTCC TCACTGACCC TGTCCTGGTA TGGCTCCTCA
    TATGATGGGG GCAGTGCTGT ACAGTCCTAC AGCATCGAGA TCTGGGACTC AGCCAACAAG
    ACGTGGAAGG AACTAGCCAC ATGCCGCAGC ACCTCTTTCA ACGTCCAGGA CCTGCTGCCT
    GACCACGAAT ATAAGTTCCG TGTACGTGCA ATCAACGTGT ATGGAACCAG TGAGCCAAGC
    CAGGAGTCTG AACTCACAAC GGTAGGAGAG AAACCTGAAG AGCCGAAGGA TGAAGTGGAG
    GTGTCAGATG ATGATGAGAA GGAGCCCGAG GTTGATTACC GGACAGTGAC AATCAATACT
    GAACAAAAAG TATCTGACTT CTACGACATT GAGGAGAGAT TAGGATCTGG GAAATTTGGA
    CAGGTCTTTC GACTTGTAGA AAAGAAAACT CGAAAAGTCT GGGCAGGGAA GTTCTTCAAG
    GCATATTCAG CAAAAGAGAA AGAGAATATC CGGCAGGAGA TTAGCATCAT GAACTGCCTC
    CACCACCCTA AGCTGGTCCA GTGTGTGGAT GCCTTTGAAG AAAAGGCCAA CATCGTCATG
    GTCCTGGAGA TCGTGTCAGG AGGGGAGCTG TTTGAGCGCA TCATTGACGA GGACTTTGAG
    CTGACGGAGC GTGAGTGCAT CAAGTACATG CGGCAGATCT CGGAGGGAGT GGAGTACATC
    CACAAGCAGG GCATCGTGCA CCTGGACCTC AAGCCGGAGA ACATCATGTG TGTCAACAAG
    ACGGGCACCA GGATCAAGCT CATCGACTTT GGTCTGGCCA GGAGGCTGGA GAATGCGGGG
    TCTCTGAAGG TCCTCTTTGG CACCCCAGAA TTTGTGGCTC CTGAAGTGAT CAACTATGAG
    CCCATCGGCT ACGCCACAGA CATGTGGAGC ATCGGGGTCA TCTGCTACAT CCTAGTCAGT
    GGCCTTTCCC CCTTCATGGG AGACAACGAT AACGAAACCT TGGCCAACGT TACCTCAGCC
    ACCTGGGACT TCGACGACGA GGCATTCGAT GAGATCTCCG ACGATGCCAA GGATTTCATC
    AGCAATCTGC TGAAGAAAGA TATGAAAAAC CGCCTGGACT GCACGCAGTG CCTTCAGCAT
    CCATGGCTAA TGAAAGATAC CAAGAACATG GAGGCCAAGA AACTCTCCAA GGACCGGATG
    AAGAAGTACA TGGCAAGAAG GAAATGGCAG AAAACGGGCA ATGCTGTGAG AGCCATTGGA
    AGACTGTCCT CTATGGCAAT GATCTCAGGG CTCAGTGGCA GGAAATCCTC AACAGGGTCA
    CCAACCAGCC CGCTCAATGC AGAAAAACTA GAATCTGAAG AAGATGTGTC CCAAGCTTTC
    CTTGAGGCTG TTGCTGAGGA AAAGCCTCAT GTAAAACCCT ATTTCTCTAA GACCATTCGC
    GATTTAGAAG TTGTGGAGGG AAGTGCTGCT AGATTTGACT GCAAGATTGA AGGATACCCA
    GACCCCGAGG TTGTCTGGTT CAAAGATGAC CAGTCAATCA GGGAGTCCCG CCACTTCCAG
    ATAGACTACG ATGAGGACGG GAACTGCTCT TTAATTATTA GTGATGTTTG CGGGGATGAC
    GATGCCAAGT ACACCTGCAA GGCTGTCAAC AGTCTTGGAG AAGCCACCTG CACAGCAGAG
    CTCATTGTGG AAACGATGGA GGAAGGTGAA GGGGAAGGGG AAGAGGAAGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16318
    Clone ID Related Accession (Same CDS sequence) NM_053026.4 , NM_053026.3 , XM_024453535.1
    Accession Version XM_024453535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAGTCAG TGGCCTTTCC CCCTTCATGG GAGACAACGA TAACGAAACC
    TTGGCCAACG TTACCTCAGC CACCTGGGAC TTCGACGACG AGGCATTCGA TGAGATCTCC
    GACGATGCCA AGGATTTCAT CAGCAATCTG CTGAAGAAAG ATATGAAAAA CCGCCTGGAC
    TGCACGCAGT GCCTTCAGCA TCCATGGCTA ATGAAAGATA CCAAGAACAT GGAGGCCAAG
    AAACTCTCCA AGGACCGGAT GAAGAAGTAC ATGGCAAGAA GGAAATGGCA GAAAACGGGC
    AATGCTGTGA GAGCCATTGG AAGACTGTCC TCTATGGCAA TGATCTCAGG GCTCAGTGGC
    AGGAAATCCT CAACAGGGTC ACCAACCAGC CCGCTCAATG CAGAAAAACT AGAATCTGAA
    GAAGATGTGT CCCAAGCTTT CCTTGAGGCT GTTGCTGAGG AAAAGCCTCA TGTAAAACCC
    TATTTCTCTA AGACCATTCG CGATTTAGAA GTTGTGGAGG GAAGTGCTGC TAGATTTGAC
    TGCAAGATTG AAGGATACCC AGACCCCGAG GTTGTCTGGT TCAAAGATGA CCAGTCAATC
    AGGGAGTCCC GCCACTTCCA GATAGACTAC GATGAGGACG GGAACTGCTC TTTAATTATT
    AGTGATGTTT GCGGGGATGA CGATGCCAAG TACACCTGCA AGGCTGTCAA CAGTCTTGGA
    GAAGCCACCT GCACAGCAGA GCTCATTGTG GAAACGATGG AGGAAGGTGA AGGGGAAGGG
    GAAGAGGAAG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309303.1
    CDS142..5679
    Translation

    Target ORF information:

    RefSeq Version XM_024453535.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGG GCTTGCCGTG
    ATGGAGGTGG CCCCCTCCTT CTCCAGTGTC CTGAAGGACT GCGCTGTTAT TGAGGGCCAG
    GATTTTGTGC TGCAGTGCTC CGTACGGGGG ACCCCAGTGC CCCGGATCAC TTGGCTGCTG
    AATGGGCAGC CCATCCAGTA CGCTCGCTCC ACCTGCGAGG CCGGCGTGGC TGAGCTCCAC
    ATCCAGGATG CCCTGCCGGA GGACCATGGC ACCTACACCT GCCTAGCTGA GAATGCCTTG
    GGGCAGGTGT CCTGCAGCGC CTGGGTCACC GTCCATGAAA AGAAGAGTAG CAGGAAGAGT
    GAGTACCTTC TGCCTGTGGC TCCCAGCAAG CCCACTGCAC CCATCTTCCT GCAGGGCCTC
    TCTGATCTCA AAGTCATGGA TGGAAGCCAG GTCACTATGA CTGTCCAAGT GTCAGGGAAT
    CCACCCCCTG AAGTCATCTG GCTGCACAAT GGGAATGAGA TCCAAGAGTC AGAGGACTTC
    CACTTTGAAC AGAGAGGAAC TCAGCACAGC CTTTGTATCC AGGAAGTGTT CCCGGAGGAC
    ACGGGCACGT ACACCTGCGA GGCCTGGAAC AGCGCTGGAG AGGTCCGCAC CCAGGCCGTG
    CTCACGGTAC AAGAGCCTCA CGATGGCACC CAGCCCTGGT TCATCAGTAA GCCTCGCTCA
    GTGACAGCCT CCCTGGGCCA GAGTGTCCTC ATCTCCTGCG CCATAGCTGG TGACCCCTTT
    CCTACCGTGC ACTGGCTCAG AGATGGCAAA GCCCTCTGCA AAGACACTGG CCACTTCGAG
    GTGCTTCAGA ATGAGGACGT GTTCACCCTG GTTCTAAAGA AGGTGCAGCC CTGGCATGCC
    GGCCAGTATG AGATCCTGCT CAAGAACCGG GTTGGCGAAT GCAGTTGCCA GGTGTCACTG
    ATGCTACAGA ACAGCTCTGC CAGAGCCCTT CCACGGGGGA GGGAGCCTGC CAGCTGCGAG
    GACCTCTGTG GTGGAGGAGT TGGTGCTGAT GGTGGTGGTA GTGACCGCTA TGGGTCCCTG
    AGGCCTGGCT GGCCAGCAAG AGGGCAGGGT TGGCTAGAGG AGGAAGACGG CGAGGACGTG
    CGAGGGGTGC TGAAGAGGCG CGTGGAGACG AGGCAGCACA CTGAGGAGGC GATCCGCCAG
    CAGGAGGTGG AGCAGCTGGA CTTCCGAGAC CTCCTGGGGA AGAAGGTGAG TACAAAGACC
    CTATCGGAAG ACGACCTGAA GGAGATCCCA GCCGAGCAGA TGGATTTCCG TGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCT GAGGAAGAGA GGAAGGTGCA CAGCCCCCAG
    CAGGTCGATT TTCGCTCTGT CCTGGCCAAG AAGGGGACTT CCAAGACCCC CGTGCCTGAG
    AAGGTGCCAC CGCCAAAACC TGCCACCCCG GATTTTCGCT CAGTGCTGGG TGGCAAGAAG
    AAATTACCAG CAGAGAATGG CAGCAGCAGT GCCGAGACCC TGAATGCCAA GGCAGTGGAG
    AGTTCCAAGC CCCTGAGCAA TGCACAGCCT TCAGGGCCCT TGAAACCCGT GGGCAACGCC
    AAGCCTGCTG AGACCCTGAA GCCAATGGGC AACGCCAAGC CTGCCGAGAC CCTGAAGCCC
    ATGGGCAATG CCAAGCCTGA TGAGAACCTG AAATCCGCTA GCAAAGAAGA ACTCAAGAAA
    GACGTTAAGA ATGATGTGAA CTGCAAGAGA GGCCATGCAG GGACCACAGA TAATGAAAAG
    AGATCAGAGA GCCAGGGGAC AGCCCCAGCC TTCAAGCAGA AGCTGCAAGA TGTTCATGTG
    GCAGAGGGCA AGAAGCTGCT GCTCCAGTGC CAGGTGTCTT CTGACCCCCC AGCCACCATC
    ATCTGGACGC TGAACGGAAA GACCCTCAAG ACCACCAAGT TCATCATCCT CTCCCAGGAA
    GGCTCACTCT GCTCCGTCTC CATCGAGAAG GCACTGCCTG AGGACAGAGG CTTATACAAG
    TGTGTAGCCA AGAATGACGC TGGCCAGGCG GAGTGCTCCT GCCAAGTCAC CGTGGATGAT
    GCTCCAGCCA GTGAGAACAC CAAGGCCCCA GAGATGAAAT CCCGGAGGCC CAAGAGCTCT
    CTTCCTCCCG TGCTAGGAAC TGAGAGTGAT GCGACTGTGA AAAAGAAACC TGCCCCCAAG
    ACACCTCCGA AGGCAGCAAT GCCCCCTCAG ATCATCCAGT TCCCTGAGGA CCAGAAGGTA
    CGCGCAGGAG AGTCAGTGGA GCTGTTTGGC AAAGTGACAG GCACTCAGCC CATCACCTGT
    ACCTGGATGA AGTTCCGAAA GCAGATCCAG GAAAGCGAGC ACATGAAGGT GGAGAACAGC
    GAGAATGGCA GCAAGCTCAC CATCCTGGCC GCGCGCCAGG AGCACTGCGG CTGCTACACA
    CTGCTGGTGG AGAACAAGCT GGGCAGCAGG CAGGCCCAGG TCAACCTCAC TGTCGTGGAT
    AAGCCAGACC CCCCAGCTGG CACACCTTGT GCCTCTGACA TTCGGAGCTC CTCACTGACC
    CTGTCCTGGT ATGGCTCCTC ATATGATGGG GGCAGTGCTG TACAGTCCTA CAGCATCGAG
    ATCTGGGACT CAGCCAACAA GACGTGGAAG GAACTAGCCA CATGCCGCAG CACCTCTTTC
    AACGTCCAGG ACCTGCTGCC TGACCACGAA TATAAGTTCC GTGTACGTGC AATCAACGTG
    TATGGAACCA GTGAGCCAAG CCAGGAGTCT GAACTCACAA CGGTAGGAGA GAAACCTGAA
    GAGCCGAAGG ATGAAGTGGA GGTGTCAGAT GATGATGAGA AGGAGCCCGA GGTTGATTAC
    CGGACAGTGA CAATCAATAC TGAACAAAAA GTATCTGACT TCTACGACAT TGAGGAGAGA
    TTAGGATCTG GGAAATTTGG ACAGGTCTTT CGACTTGTAG AAAAGAAAAC TCGAAAAGTC
    TGGGCAGGGA AGTTCTTCAA GGCATATTCA GCAAAAGAGA AAGAGAATAT CCGGCAGGAG
    ATTAGCATCA TGAACTGCCT CCACCACCCT AAGCTGGTCC AGTGTGTGGA TGCCTTTGAA
    GAAAAGGCCA ACATCGTCAT GGTCCTGGAG ATCGTGTCAG GAGGGGAGCT GTTTGAGCGC
    ATCATTGACG AGGACTTTGA GCTGACGGAG CGTGAGTGCA TCAAGTACAT GCGGCAGATC
    TCGGAGGGAG TGGAGTACAT CCACAAGCAG GGCATCGTGC ACCTGGACCT CAAGCCGGAG
    AACATCATGT GTGTCAACAA GACGGGCACC AGGATCAAGC TCATCGACTT TGGTCTGGCC
    AGGAGGCTGG AGAATGCGGG GTCTCTGAAG GTCCTCTTTG GCACCCCAGA ATTTGTGGCT
    CCTGAAGTGA TCAACTATGA GCCCATCGGC TACGCCACAG ACATGTGGAG CATCGGGGTC
    ATCTGCTACA TCCTAGTCAG TGGCCTTTCC CCCTTCATGG GAGACAACGA TAACGAAACC
    TTGGCCAACG TTACCTCAGC CACCTGGGAC TTCGACGACG AGGCATTCGA TGAGATCTCC
    GACGATGCCA AGGATTTCAT CAGCAATCTG CTGAAGAAAG ATATGAAAAA CCGCCTGGAC
    TGCACGCAGT GCCTTCAGCA TCCATGGCTA ATGAAAGATA CCAAGAACAT GGAGGCCAAG
    AAACTCTCCA AGGACCGGAT GAAGAAGTAC ATGGCAAGAA GGAAATGGCA GAAAACGGGC
    AATGCTGTGA GAGCCATTGG AAGACTGTCC TCTATGGCAA TGATCTCAGG GCTCAGTGGC
    AGGAAATCCT CAACAGGGTC ACCAACCAGC CCGCTCAATG CAGAAAAACT AGAATCTGAA
    GAAGATGTGT CCCAAGCTTT CCTTGAGGCT GTTGCTGAGG AAAAGCCTCA TGTAAAACCC
    TATTTCTCTA AGACCATTCG CGATTTAGAA GTTGTGGAGG GAAGTGCTGC TAGATTTGAC
    TGCAAGATTG AAGGATACCC AGACCCCGAG GTTGTCTGGT TCAAAGATGA CCAGTCAATC
    AGGGAGTCCC GCCACTTCCA GATAGACTAC GATGAGGACG GGAACTGCTC TTTAATTATT
    AGTGATGTTT GCGGGGATGA CGATGCCAAG TACACCTGCA AGGCTGTCAA CAGTCTTGGA
    GAAGCCACCT GCACAGCAGA GCTCATTGTG GAAACGATGG AGGAAGGTGA AGGGGAAGGG
    GAAGAGGAAG AAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17800
    Clone ID Related Accession (Same CDS sequence) NM_053025.4 , NM_053025.3 , XM_024453536.1 , XM_024453537.1 , XM_024453533.1
    Accession Version XM_024453536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309304.1
    CDS355..6099
    Translation

    Target ORF information:

    RefSeq Version XM_024453536.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17800
    Clone ID Related Accession (Same CDS sequence) NM_053025.4 , NM_053025.3 , XM_024453536.1 , XM_024453537.1 , XM_024453533.1
    Accession Version XM_024453537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309305.1
    CDS336..6080
    Translation

    Target ORF information:

    RefSeq Version XM_024453537.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17800
    Clone ID Related Accession (Same CDS sequence) NM_053025.4 , NM_053025.3 , XM_024453536.1 , XM_024453537.1 , XM_024453533.1
    Accession Version XM_024453533.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309301.1
    CDS144..5888
    Translation

    Target ORF information:

    RefSeq Version XM_024453533.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453533.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCTCATGT AAAACCCTAT TTCTCTAAGA CCATTCGCGA TTTAGAAGTT
    GTGGAGGGAA GTGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCCGAGGTT
    GTCTGGTTCA AAGATGACCA GTCAATCAGG GAGTCCCGCC ACTTCCAGAT AGACTACGAT
    GAGGACGGGA ACTGCTCTTT AATTATTAGT GATGTTTGCG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAACAG TCTTGGAGAA GCCACCTGCA CAGCAGAGCT CATTGTGGAA
    ACGATGGAGG AAGGTGAAGG GGAAGGGGAA GAGGAAGAAG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59145
    Clone ID Related Accession (Same CDS sequence) XM_011512860.2
    Accession Version XM_011512860.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511162.1
    CDS4..5745
    Translation

    Target ORF information:

    RefSeq Version XM_011512860.2
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011512860.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCTGGGA AATTTGGACA GGTCTTTCGA
    CTTGTAGAAA AGAAAACTCG AAAAGTCTGG GCAGGGAAGT TCTTCAAGGC ATATTCAGCA
    AAAGAGAAAG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCTAAG
    CTGGTCCAGT GTGTGGATGC CTTTGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCAGGAG GGGAGCTGTT TGAGCGCATC ATTGACGAGG ACTTTGAGCT GACGGAGCGT
    GAGTGCATCA AGTACATGCG GCAGATCTCG GAGGGAGTGG AGTACATCCA CAAGCAGGGC
    ATCGTGCACC TGGACCTCAA GCCGGAGAAC ATCATGTGTG TCAACAAGAC GGGCACCAGG
    ATCAAGCTCA TCGACTTTGG TCTGGCCAGG AGGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGCA CCCCAGAATT TGTGGCTCCT GAAGTGATCA ACTATGAGCC CATCGGCTAC
    GCCACAGACA TGTGGAGCAT CGGGGTCATC TGCTACATCC TAGTCAGTGG CCTTTCCCCC
    TTCATGGGAG ACAACGATAA CGAAACCTTG GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GACGACGAGG CATTCGATGA GATCTCCGAC GATGCCAAGG ATTTCATCAG CAATCTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGC ACGCAGTGCC TTCAGCATCC ATGGCTAATG
    AAAGATACCA AGAACATGGA GGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATTGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGGTCACC AACCAGCCCG
    CTCAATGCAG AAAAACTAGA ATCTGAAGAT GTGTCCCAAG CTTTCCTTGA GGCTGTTGCT
    GAGGAAAAGC CTCATGTAAA ACCCTATTTC TCTAAGACCA TTCGCGATTT AGAAGTTGTG
    GAGGGAAGTG CTGCTAGATT TGACTGCAAG ATTGAAGGAT ACCCAGACCC CGAGGTTGTC
    TGGTTCAAAG ATGACCAGTC AATCAGGGAG TCCCGCCACT TCCAGATAGA CTACGATGAG
    GACGGGAACT GCTCTTTAAT TATTAGTGAT GTTTGCGGGG ATGACGATGC CAAGTACACC
    TGCAAGGCTG TCAACAGTCT TGGAGAAGCC ACCTGCACAG CAGAGCTCAT TGTGGAAACG
    ATGGAGGAAG GTGAAGGGGA AGGGGAAGAG GAAGAAGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59146
    Clone ID Related Accession (Same CDS sequence) XM_011512861.2
    Accession Version XM_011512861.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCCGTGT CAGGAGGGGA GCTGTTTGAG
    CGCATCATTG ACGAGGACTT TGAGCTGACG GAGCGTGAGT GCATCAAGTA CATGCGGCAG
    ATCTCGGAGG GAGTGGAGTA CATCCACAAG CAGGGCATCG TGCACCTGGA CCTCAAGCCG
    GAGAACATCA TGTGTGTCAA CAAGACGGGC ACCAGGATCA AGCTCATCGA CTTTGGTCTG
    GCCAGGAGGC TGGAGAATGC GGGGTCTCTG AAGGTCCTCT TTGGCACCCC AGAATTTGTG
    GCTCCTGAAG TGATCAACTA TGAGCCCATC GGCTACGCCA CAGACATGTG GAGCATCGGG
    GTCATCTGCT ACATCCTAGT CAGTGGCCTT TCCCCCTTCA TGGGAGACAA CGATAACGAA
    ACCTTGGCCA ACGTTACCTC AGCCACCTGG GACTTCGACG ACGAGGCATT CGATGAGATC
    TCCGACGATG CCAAGGATTT CATCAGCAAT CTGCTGAAGA AAGATATGAA AAACCGCCTG
    GACTGCACGC AGTGCCTTCA GCATCCATGG CTAATGAAAG ATACCAAGAA CATGGAGGCC
    AAGAAACTCT CCAAGGACCG GATGAAGAAG TACATGGCAA GAAGGAAATG GCAGAAAACG
    GGCAATGCTG TGAGAGCCAT TGGAAGACTG TCCTCTATGG CAATGATCTC AGGGCTCAGT
    GGCAGGAAAT CCTCAACAGG GTCACCAACC AGCCCGCTCA ATGCAGAAAA ACTAGAATCT
    GAAGAAGATG TGTCCCAAGC TTTCCTTGAG GCTGTTGCTG AGGAAAAGCC TCATGTAAAA
    CCCTATTTCT CTAAGACCAT TCGCGATTTA GAAGTTGTGG AGGGAAGTGC TGCTAGATTT
    GACTGCAAGA TTGAAGGATA CCCAGACCCC GAGGTTGTCT GGTTCAAAGA TGACCAGTCA
    ATCAGGGAGT CCCGCCACTT CCAGATAGAC TACGATGAGG ACGGGAACTG CTCTTTAATT
    ATTAGTGATG TTTGCGGGGA TGACGATGCC AAGTACACCT GCAAGGCTGT CAACAGTCTT
    GGAGAAGCCA CCTGCACAGC AGAGCTCATT GTGGAAACGA TGGAGGAAGG TGAAGGGGAA
    GGGGAAGAGG AAGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511163.1
    CDS4..5544
    Translation

    Target ORF information:

    RefSeq Version XM_011512861.2
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011512861.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGGGATG TGAAGCTGGT TGCCTCGTCA CACATTTCCA AAACCTCCCT CAGTGTGGAT 
    CCCTCAAGAG TTGACTCCAT GCCCCTGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAAAGAAGG AGCCACCGCC AAGTTCGAAG GGCGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CAGAAACGGG CAACCCATCA CCAGCGGGGG CCGCTTCCTG
    CTGGATTGCG GCATCCGGGG GACTTTCAGC CTTGTGATTC ATGCTGTCCA TGAGGAGGAC
    AGGGGAAAGT ATACCTGTGA AGCCACCAAT GGCAGTGGTG CTCGCCAGGT GACAGTGGAG
    TTGACAGTAG AAGGAAGTTT TGCGAAGCAG CTTGGTCAGC CTGTTGTTTC CAAAACCTTA
    GGGGATAGAT TTTCAGCTCC AGCAGTGGAG ACCCGTCCTA GCATCTGGGG GGAGTGCCCA
    CCAAAGTTTG CTACCAAGCT GGGCCGAGTT GTGGTCAAAG AAGGACAGAT GGGACGATTC
    TCCTGCAAGA TCACTGGCCG GCCCCAACCG CAGGTCACCT GGCTCAAGGG AAATGTTCCA
    CTGCAGCCGA GTGCCCGTGT GTCTGTGTCT GAGAAGAACG GCATGCAGGT TCTGGAAATC
    CATGGAGTCA ACCAAGATGA CGTGGGAGTG TACACGTGCC TGGTGGTGAA CGGGTCGGGG
    AAGGCCTCGA TGTCAGCTGA ACTTTCCATC CAAGGTTTGG ACAGTGCCAA TAGGTCATTT
    GTGAGAGAAA CAAAAGCCAC CAATTCAGAT GTCAGGAAAG AGGTGACCAA TGTAATCTCA
    AAGGAGTCGA AGCTGGACAG TCTGGAGGCT GCAGCCAAAA GCAAGAACTG CTCCAGCCCC
    CAGAGAGGTG GCTCCCCACC CTGGGCTGCA AACAGCCAGC CTCAGCCCCC AAGGGAGTCC
    AAGCTGGAGT CATGCAAGGA CTCGCCCAGA ACGGCCCCGC AGACCCCGGT CCTTCAGAAG
    ACTTCCAGCT CCATCACCCT GCAGGCCGCA AGAGTTCAGC CGGAACCAAG AGCACCAGGC
    CTGGGGGTCC TATCACCTTC TGGAGAAGAG AGGAAGAGGC CAGCTCCTCC CCGTCCAGCC
    ACCTTCCCCA CCAGGCAGCC TGGCCTGGGG AGCCAAGATG TTGTGAGCAA GGCTGCTAAC
    AGGAGAATCC CCATGGAGGG CCAGAGGGAT TCAGCATTCC CCAAATTTGA GAGCAAGCCC
    CAAAGCCAGG AGGTCAAGGA AAATCAAACT GTCAAGTTCA GATGTGAAGT TTCCGGGATT
    CCAAAGCCTG AAGTGGCCTG GTTCCTGGAA GGCACCCCCG TGAGGAGACA GGAAGGCAGC
    ATTGAGGTTT ATGAAGATGC TGGCTCCCAT TACCTCTGCC TGCTGAAAGC CCGGACCAGG
    GACAGTGGGA CATACAGCTG CACTGCTTCC AACGCCCAAG GCCAGCTGTC CTGTAGCTGG
    ACCCTCCAAG TGGAAAGGCT TGCCGTGATG GAGGTGGCCC CCTCCTTCTC CAGTGTCCTG
    AAGGACTGCG CTGTTATTGA GGGCCAGGAT TTTGTGCTGC AGTGCTCCGT ACGGGGGACC
    CCAGTGCCCC GGATCACTTG GCTGCTGAAT GGGCAGCCCA TCCAGTACGC TCGCTCCACC
    TGCGAGGCCG GCGTGGCTGA GCTCCACATC CAGGATGCCC TGCCGGAGGA CCATGGCACC
    TACACCTGCC TAGCTGAGAA TGCCTTGGGG CAGGTGTCCT GCAGCGCCTG GGTCACCGTC
    CATGAAAAGA AGAGTAGCAG GAAGAGTGAG TACCTTCTGC CTGTGGCTCC CAGCAAGCCC
    ACTGCACCCA TCTTCCTGCA GGGCCTCTCT GATCTCAAAG TCATGGATGG AAGCCAGGTC
    ACTATGACTG TCCAAGTGTC AGGGAATCCA CCCCCTGAAG TCATCTGGCT GCACAATGGG
    AATGAGATCC AAGAGTCAGA GGACTTCCAC TTTGAACAGA GAGGAACTCA GCACAGCCTT
    TGTATCCAGG AAGTGTTCCC GGAGGACACG GGCACGTACA CCTGCGAGGC CTGGAACAGC
    GCTGGAGAGG TCCGCACCCA GGCCGTGCTC ACGGTACAAG AGCCTCACGA TGGCACCCAG
    CCCTGGTTCA TCAGTAAGCC TCGCTCAGTG ACAGCCTCCC TGGGCCAGAG TGTCCTCATC
    TCCTGCGCCA TAGCTGGTGA CCCCTTTCCT ACCGTGCACT GGCTCAGAGA TGGCAAAGCC
    CTCTGCAAAG ACACTGGCCA CTTCGAGGTG CTTCAGAATG AGGACGTGTT CACCCTGGTT
    CTAAAGAAGG TGCAGCCCTG GCATGCCGGC CAGTATGAGA TCCTGCTCAA GAACCGGGTT
    GGCGAATGCA GTTGCCAGGT GTCACTGATG CTACAGAACA GCTCTGCCAG AGCCCTTCCA
    CGGGGGAGGG AGCCTGCCAG CTGCGAGGAC CTCTGTGGTG GAGGAGTTGG TGCTGATGGT
    GGTGGTAGTG ACCGCTATGG GTCCCTGAGG CCTGGCTGGC CAGCAAGAGG GCAGGGTTGG
    CTAGAGGAGG AAGACGGCGA GGACGTGCGA GGGGTGCTGA AGAGGCGCGT GGAGACGAGG
    CAGCACACTG AGGAGGCGAT CCGCCAGCAG GAGGTGGAGC AGCTGGACTT CCGAGACCTC
    CTGGGGAAGA AGGTGAGTAC AAAGACCCTA TCGGAAGACG ACCTGAAGGA GATCCCAGCC
    GAGCAGATGG ATTTCCGTGC CAACCTGCAG CGGCAAGTGA AGCCAAAGAC TGTGTCTGAG
    GAAGAGAGGA AGGTGCACAG CCCCCAGCAG GTCGATTTTC GCTCTGTCCT GGCCAAGAAG
    GGGACTTCCA AGACCCCCGT GCCTGAGAAG GTGCCACCGC CAAAACCTGC CACCCCGGAT
    TTTCGCTCAG TGCTGGGTGG CAAGAAGAAA TTACCAGCAG AGAATGGCAG CAGCAGTGCC
    GAGACCCTGA ATGCCAAGGC AGTGGAGAGT TCCAAGCCCC TGAGCAATGC ACAGCCTTCA
    GGGCCCTTGA AACCCGTGGG CAACGCCAAG CCTGCTGAGA CCCTGAAGCC AATGGGCAAC
    GCCAAGCCTG CCGAGACCCT GAAGCCCATG GGCAATGCCA AGCCTGATGA GAACCTGAAA
    TCCGCTAGCA AAGAAGAACT CAAGAAAGAC GTTAAGAATG ATGTGAACTG CAAGAGAGGC
    CATGCAGGGA CCACAGATAA TGAAAAGAGA TCAGAGAGCC AGGGGACAGC CCCAGCCTTC
    AAGCAGAAGC TGCAAGATGT TCATGTGGCA GAGGGCAAGA AGCTGCTGCT CCAGTGCCAG
    GTGTCTTCTG ACCCCCCAGC CACCATCATC TGGACGCTGA ACGGAAAGAC CCTCAAGACC
    ACCAAGTTCA TCATCCTCTC CCAGGAAGGC TCACTCTGCT CCGTCTCCAT CGAGAAGGCA
    CTGCCTGAGG ACAGAGGCTT ATACAAGTGT GTAGCCAAGA ATGACGCTGG CCAGGCGGAG
    TGCTCCTGCC AAGTCACCGT GGATGATGCT CCAGCCAGTG AGAACACCAA GGCCCCAGAG
    ATGAAATCCC GGAGGCCCAA GAGCTCTCTT CCTCCCGTGC TAGGAACTGA GAGTGATGCG
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCGAAGG CAGCAATGCC CCCTCAGATC
    ATCCAGTTCC CTGAGGACCA GAAGGTACGC GCAGGAGAGT CAGTGGAGCT GTTTGGCAAA
    GTGACAGGCA CTCAGCCCAT CACCTGTACC TGGATGAAGT TCCGAAAGCA GATCCAGGAA
    AGCGAGCACA TGAAGGTGGA GAACAGCGAG AATGGCAGCA AGCTCACCAT CCTGGCCGCG
    CGCCAGGAGC ACTGCGGCTG CTACACACTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGATAAG CCAGACCCCC CAGCTGGCAC ACCTTGTGCC
    TCTGACATTC GGAGCTCCTC ACTGACCCTG TCCTGGTATG GCTCCTCATA TGATGGGGGC
    AGTGCTGTAC AGTCCTACAG CATCGAGATC TGGGACTCAG CCAACAAGAC GTGGAAGGAA
    CTAGCCACAT GCCGCAGCAC CTCTTTCAAC GTCCAGGACC TGCTGCCTGA CCACGAATAT
    AAGTTCCGTG TACGTGCAAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAACGG TAGGAGAGAA ACCTGAAGAG CCGAAGGATG AAGTGGAGGT GTCAGATGAT
    GATGAGAAGG AGCCCGAGGT TGATTACCGG ACAGTGACAA TCAATACTGA ACAAAAAGTA
    TCTGACTTCT ACGACATTGA GGAGAGATTA GGATCCGTGT CAGGAGGGGA GCTGTTTGAG
    CGCATCATTG ACGAGGACTT TGAGCTGACG GAGCGTGAGT GCATCAAGTA CATGCGGCAG
    ATCTCGGAGG GAGTGGAGTA CATCCACAAG CAGGGCATCG TGCACCTGGA CCTCAAGCCG
    GAGAACATCA TGTGTGTCAA CAAGACGGGC ACCAGGATCA AGCTCATCGA CTTTGGTCTG
    GCCAGGAGGC TGGAGAATGC GGGGTCTCTG AAGGTCCTCT TTGGCACCCC AGAATTTGTG
    GCTCCTGAAG TGATCAACTA TGAGCCCATC GGCTACGCCA CAGACATGTG GAGCATCGGG
    GTCATCTGCT ACATCCTAGT CAGTGGCCTT TCCCCCTTCA TGGGAGACAA CGATAACGAA
    ACCTTGGCCA ACGTTACCTC AGCCACCTGG GACTTCGACG ACGAGGCATT CGATGAGATC
    TCCGACGATG CCAAGGATTT CATCAGCAAT CTGCTGAAGA AAGATATGAA AAACCGCCTG
    GACTGCACGC AGTGCCTTCA GCATCCATGG CTAATGAAAG ATACCAAGAA CATGGAGGCC
    AAGAAACTCT CCAAGGACCG GATGAAGAAG TACATGGCAA GAAGGAAATG GCAGAAAACG
    GGCAATGCTG TGAGAGCCAT TGGAAGACTG TCCTCTATGG CAATGATCTC AGGGCTCAGT
    GGCAGGAAAT CCTCAACAGG GTCACCAACC AGCCCGCTCA ATGCAGAAAA ACTAGAATCT
    GAAGAAGATG TGTCCCAAGC TTTCCTTGAG GCTGTTGCTG AGGAAAAGCC TCATGTAAAA
    CCCTATTTCT CTAAGACCAT TCGCGATTTA GAAGTTGTGG AGGGAAGTGC TGCTAGATTT
    GACTGCAAGA TTGAAGGATA CCCAGACCCC GAGGTTGTCT GGTTCAAAGA TGACCAGTCA
    ATCAGGGAGT CCCGCCACTT CCAGATAGAC TACGATGAGG ACGGGAACTG CTCTTTAATT
    ATTAGTGATG TTTGCGGGGA TGACGATGCC AAGTACACCT GCAAGGCTGT CAACAGTCTT
    GGAGAAGCCA CCTGCACAGC AGAGCTCATT GTGGAAACGA TGGAGGAAGG TGAAGGGGAA
    GGGGAAGAGG AAGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94222
    Clone ID Related Accession (Same CDS sequence) XM_017006469.1 , XM_017006469.2
    Accession Version XM_017006469.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2976bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATTTCC GTGCCAACCT GCAGCGGCAA GTGAAGCCAA AGACTGTGTC TGAGGAAGAG 
    AGGAAGGTGC ACAGCCCCCA GCAGGTCGAT TTTCGCTCTG TCCTGGCCAA GAAGGGGACT
    TCCAAGACCC CCGTGCCTGA GAAGGTGCCA CCGCCAAAAC CTGCCACCCC GGATTTTCGC
    TCAGTGCTGG GTGGCAAGAA GAAATTACCA GCAGAGAATG GCAGCAGCAG TGCCGAGACC
    CTGAATGCCA AGGCAGTGGA GAGTTCCAAG CCCCTGAGCA ATGCACAGCC TTCAGGGCCC
    TTGAAACCCG TGGGCAACGC CAAGCCTGCT GAGACCCTGA AGCCAATGGG CAACGCCAAG
    CCTGCCGAGA CCCTGAAGCC CATGGGCAAT GCCAAGCCTG ATGAGAACCT GAAATCCGCT
    AGCAAAGAAG AACTCAAGAA AGACGTTAAG AATGATGTGA ACTGCAAGAG AGGCCATGCA
    GGGACCACAG ATAATGAAAA GAGATCAGAG AGCCAGGGGA CAGCCCCAGC CTTCAAGCAG
    AAGCTGCAAG ATGTTCATGT GGCAGAGGGC AAGAAGCTGC TGCTCCAGTG CCAGGTGTCT
    TCTGACCCCC CAGCCACCAT CATCTGGACG CTGAACGGAA AGACCCTCAA GACCACCAAG
    TTCATCATCC TCTCCCAGGA AGGCTCACTC TGCTCCGTCT CCATCGAGAA GGCACTGCCT
    GAGGACAGAG GCTTATACAA GTGTGTAGCC AAGAATGACG CTGGCCAGGC GGAGTGCTCC
    TGCCAAGTCA CCGTGGATGA TGCTCCAGCC AGTGAGAACA CCAAGGCCCC AGAGATGAAA
    TCCCGGAGGC CCAAGAGCTC TCTTCCTCCC GTGCTAGGAA CTGAGAGTGA TGCGACTGTG
    AAAAAGAAAC CTGCCCCCAA GACACCTCCG AAGGCAGCAA TGCCCCCTCA GATCATCCAG
    TTCCCTGAGG ACCAGAAGGT ACGCGCAGGA GAGTCAGTGG AGCTGTTTGG CAAAGTGACA
    GGCACTCAGC CCATCACCTG TACCTGGATG AAGTTCCGAA AGCAGATCCA GGAAAGCGAG
    CACATGAAGG TGGAGAACAG CGAGAATGGC AGCAAGCTCA CCATCCTGGC CGCGCGCCAG
    GAGCACTGCG GCTGCTACAC ACTGCTGGTG GAGAACAAGC TGGGCAGCAG GCAGGCCCAG
    GTCAACCTCA CTGTCGTGGA TAAGCCAGAC CCCCCAGCTG GCACACCTTG TGCCTCTGAC
    ATTCGGAGCT CCTCACTGAC CCTGTCCTGG TATGGCTCCT CATATGATGG GGGCAGTGCT
    GTACAGTCCT ACAGCATCGA GATCTGGGAC TCAGCCAACA AGACGTGGAA GGAACTAGCC
    ACATGCCGCA GCACCTCTTT CAACGTCCAG GACCTGCTGC CTGACCACGA ATATAAGTTC
    CGTGTACGTG CAATCAACGT GTATGGAACC AGTGAGCCAA GCCAGGAGTC TGAACTCACA
    ACGGTAGGAG AGAAACCTGA AGAGCCGAAG GATGAAGTGG AGGTGTCAGA TGATGATGAG
    AAGGAGCCCG AGGTTGATTA CCGGACAGTG ACAATCAATA CTGAACAAAA AGTATCTGAC
    TTCTACGACA TTGAGGAGAG ATTAGGATCT GGGAAATTTG GACAGGTCTT TCGACTTGTA
    GAAAAGAAAA CTCGAAAAGT CTGGGCAGGG AAGTTCTTCA AGGCATATTC AGCAAAAGAG
    AAAGAGAATA TCCGGCAGGA GATTAGCATC ATGAACTGCC TCCACCACCC TAAGCTGGTC
    CAGTGTGTGG ATGCCTTTGA AGAAAAGGCC AACATCGTCA TGGTCCTGGA GATCGTGTCA
    GGAGGGGAGC TGTTTGAGCG CATCATTGAC GAGGACTTTG AGCTGACGGA GCGTGAGTGC
    ATCAAGTACA TGCGGCAGAT CTCGGAGGGA GTGGAGTACA TCCACAAGCA GGGCATCGTG
    CACCTGGACC TCAAGCCGGA GAACATCATG TGTGTCAACA AGACGGGCAC CAGGATCAAG
    CTCATCGACT TTGGTCTGGC CAGGAGGCTG GAGAATGCGG GGTCTCTGAA GGTCCTCTTT
    GGCACCCCAG AATTTGTGGC TCCTGAAGTG ATCAACTATG AGCCCATCGG CTACGCCACA
    GACATGTGGA GCATCGGGGT CATCTGCTAC ATCCTAGTCA GTGGCCTTTC CCCCTTCATG
    GGAGACAACG ATAACGAAAC CTTGGCCAAC GTTACCTCAG CCACCTGGGA CTTCGACGAC
    GAGGCATTCG ATGAGATCTC CGACGATGCC AAGGATTTCA TCAGCAATCT GCTGAAGAAA
    GATATGAAAA ACCGCCTGGA CTGCACGCAG TGCCTTCAGC ATCCATGGCT AATGAAAGAT
    ACCAAGAACA TGGAGGCCAA GAAACTCTCC AAGGACCGGA TGAAGAAGTA CATGGCAAGA
    AGGAAATGGC AGAAAACGGG CAATGCTGTG AGAGCCATTG GAAGACTGTC CTCTATGGCA
    ATGATCTCAG GGCTCAGTGG CAGGAAATCC TCAACAGGGT CACCAACCAG CCCGCTCAAT
    GCAGAAAAAC TAGAATCTGA AGATGTGTCC CAAGCTTTCC TTGAGGCTGT TGCTGAGGAA
    AAGCCTCATG TAAAACCCTA TTTCTCTAAG ACCATTCGCG ATTTAGAAGT TGTGGAGGGA
    AGTGCTGCTA GATTTGACTG CAAGATTGAA GGATACCCAG ACCCCGAGGT TGTCTGGTTC
    AAAGATGACC AGTCAATCAG GGAGTCCCGC CACTTCCAGA TAGACTACGA TGAGGACGGG
    AACTGCTCTT TAATTATTAG TGATGTTTGC GGGGATGACG ATGCCAAGTA CACCTGCAAG
    GCTGTCAACA GTCTTGGAGA AGCCACCTGC ACAGCAGAGC TCATTGTGGA AACGATGGAG
    GAAGGTGAAG GGGAAGGGGA AGAGGAAGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861958.1
    CDS328..3303
    Translation

    Target ORF information:

    RefSeq Version XM_017006469.1
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006469.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATTTCC GTGCCAACCT GCAGCGGCAA GTGAAGCCAA AGACTGTGTC TGAGGAAGAG 
    AGGAAGGTGC ACAGCCCCCA GCAGGTCGAT TTTCGCTCTG TCCTGGCCAA GAAGGGGACT
    TCCAAGACCC CCGTGCCTGA GAAGGTGCCA CCGCCAAAAC CTGCCACCCC GGATTTTCGC
    TCAGTGCTGG GTGGCAAGAA GAAATTACCA GCAGAGAATG GCAGCAGCAG TGCCGAGACC
    CTGAATGCCA AGGCAGTGGA GAGTTCCAAG CCCCTGAGCA ATGCACAGCC TTCAGGGCCC
    TTGAAACCCG TGGGCAACGC CAAGCCTGCT GAGACCCTGA AGCCAATGGG CAACGCCAAG
    CCTGCCGAGA CCCTGAAGCC CATGGGCAAT GCCAAGCCTG ATGAGAACCT GAAATCCGCT
    AGCAAAGAAG AACTCAAGAA AGACGTTAAG AATGATGTGA ACTGCAAGAG AGGCCATGCA
    GGGACCACAG ATAATGAAAA GAGATCAGAG AGCCAGGGGA CAGCCCCAGC CTTCAAGCAG
    AAGCTGCAAG ATGTTCATGT GGCAGAGGGC AAGAAGCTGC TGCTCCAGTG CCAGGTGTCT
    TCTGACCCCC CAGCCACCAT CATCTGGACG CTGAACGGAA AGACCCTCAA GACCACCAAG
    TTCATCATCC TCTCCCAGGA AGGCTCACTC TGCTCCGTCT CCATCGAGAA GGCACTGCCT
    GAGGACAGAG GCTTATACAA GTGTGTAGCC AAGAATGACG CTGGCCAGGC GGAGTGCTCC
    TGCCAAGTCA CCGTGGATGA TGCTCCAGCC AGTGAGAACA CCAAGGCCCC AGAGATGAAA
    TCCCGGAGGC CCAAGAGCTC TCTTCCTCCC GTGCTAGGAA CTGAGAGTGA TGCGACTGTG
    AAAAAGAAAC CTGCCCCCAA GACACCTCCG AAGGCAGCAA TGCCCCCTCA GATCATCCAG
    TTCCCTGAGG ACCAGAAGGT ACGCGCAGGA GAGTCAGTGG AGCTGTTTGG CAAAGTGACA
    GGCACTCAGC CCATCACCTG TACCTGGATG AAGTTCCGAA AGCAGATCCA GGAAAGCGAG
    CACATGAAGG TGGAGAACAG CGAGAATGGC AGCAAGCTCA CCATCCTGGC CGCGCGCCAG
    GAGCACTGCG GCTGCTACAC ACTGCTGGTG GAGAACAAGC TGGGCAGCAG GCAGGCCCAG
    GTCAACCTCA CTGTCGTGGA TAAGCCAGAC CCCCCAGCTG GCACACCTTG TGCCTCTGAC
    ATTCGGAGCT CCTCACTGAC CCTGTCCTGG TATGGCTCCT CATATGATGG GGGCAGTGCT
    GTACAGTCCT ACAGCATCGA GATCTGGGAC TCAGCCAACA AGACGTGGAA GGAACTAGCC
    ACATGCCGCA GCACCTCTTT CAACGTCCAG GACCTGCTGC CTGACCACGA ATATAAGTTC
    CGTGTACGTG CAATCAACGT GTATGGAACC AGTGAGCCAA GCCAGGAGTC TGAACTCACA
    ACGGTAGGAG AGAAACCTGA AGAGCCGAAG GATGAAGTGG AGGTGTCAGA TGATGATGAG
    AAGGAGCCCG AGGTTGATTA CCGGACAGTG ACAATCAATA CTGAACAAAA AGTATCTGAC
    TTCTACGACA TTGAGGAGAG ATTAGGATCT GGGAAATTTG GACAGGTCTT TCGACTTGTA
    GAAAAGAAAA CTCGAAAAGT CTGGGCAGGG AAGTTCTTCA AGGCATATTC AGCAAAAGAG
    AAAGAGAATA TCCGGCAGGA GATTAGCATC ATGAACTGCC TCCACCACCC TAAGCTGGTC
    CAGTGTGTGG ATGCCTTTGA AGAAAAGGCC AACATCGTCA TGGTCCTGGA GATCGTGTCA
    GGAGGGGAGC TGTTTGAGCG CATCATTGAC GAGGACTTTG AGCTGACGGA GCGTGAGTGC
    ATCAAGTACA TGCGGCAGAT CTCGGAGGGA GTGGAGTACA TCCACAAGCA GGGCATCGTG
    CACCTGGACC TCAAGCCGGA GAACATCATG TGTGTCAACA AGACGGGCAC CAGGATCAAG
    CTCATCGACT TTGGTCTGGC CAGGAGGCTG GAGAATGCGG GGTCTCTGAA GGTCCTCTTT
    GGCACCCCAG AATTTGTGGC TCCTGAAGTG ATCAACTATG AGCCCATCGG CTACGCCACA
    GACATGTGGA GCATCGGGGT CATCTGCTAC ATCCTAGTCA GTGGCCTTTC CCCCTTCATG
    GGAGACAACG ATAACGAAAC CTTGGCCAAC GTTACCTCAG CCACCTGGGA CTTCGACGAC
    GAGGCATTCG ATGAGATCTC CGACGATGCC AAGGATTTCA TCAGCAATCT GCTGAAGAAA
    GATATGAAAA ACCGCCTGGA CTGCACGCAG TGCCTTCAGC ATCCATGGCT AATGAAAGAT
    ACCAAGAACA TGGAGGCCAA GAAACTCTCC AAGGACCGGA TGAAGAAGTA CATGGCAAGA
    AGGAAATGGC AGAAAACGGG CAATGCTGTG AGAGCCATTG GAAGACTGTC CTCTATGGCA
    ATGATCTCAG GGCTCAGTGG CAGGAAATCC TCAACAGGGT CACCAACCAG CCCGCTCAAT
    GCAGAAAAAC TAGAATCTGA AGATGTGTCC CAAGCTTTCC TTGAGGCTGT TGCTGAGGAA
    AAGCCTCATG TAAAACCCTA TTTCTCTAAG ACCATTCGCG ATTTAGAAGT TGTGGAGGGA
    AGTGCTGCTA GATTTGACTG CAAGATTGAA GGATACCCAG ACCCCGAGGT TGTCTGGTTC
    AAAGATGACC AGTCAATCAG GGAGTCCCGC CACTTCCAGA TAGACTACGA TGAGGACGGG
    AACTGCTCTT TAATTATTAG TGATGTTTGC GGGGATGACG ATGCCAAGTA CACCTGCAAG
    GCTGTCAACA GTCTTGGAGA AGCCACCTGC ACAGCAGAGC TCATTGTGGA AACGATGGAG
    GAAGGTGAAG GGGAAGGGGA AGAGGAAGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94222
    Clone ID Related Accession (Same CDS sequence) XM_017006469.1 , XM_017006469.2
    Accession Version XM_017006469.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2976bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product myosin light chain kinase, smooth muscle isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATTTCC GTGCCAACCT GCAGCGGCAA GTGAAGCCAA AGACTGTGTC TGAGGAAGAG 
    AGGAAGGTGC ACAGCCCCCA GCAGGTCGAT TTTCGCTCTG TCCTGGCCAA GAAGGGGACT
    TCCAAGACCC CCGTGCCTGA GAAGGTGCCA CCGCCAAAAC CTGCCACCCC GGATTTTCGC
    TCAGTGCTGG GTGGCAAGAA GAAATTACCA GCAGAGAATG GCAGCAGCAG TGCCGAGACC
    CTGAATGCCA AGGCAGTGGA GAGTTCCAAG CCCCTGAGCA ATGCACAGCC TTCAGGGCCC
    TTGAAACCCG TGGGCAACGC CAAGCCTGCT GAGACCCTGA AGCCAATGGG CAACGCCAAG
    CCTGCCGAGA CCCTGAAGCC CATGGGCAAT GCCAAGCCTG ATGAGAACCT GAAATCCGCT
    AGCAAAGAAG AACTCAAGAA AGACGTTAAG AATGATGTGA ACTGCAAGAG AGGCCATGCA
    GGGACCACAG ATAATGAAAA GAGATCAGAG AGCCAGGGGA CAGCCCCAGC CTTCAAGCAG
    AAGCTGCAAG ATGTTCATGT GGCAGAGGGC AAGAAGCTGC TGCTCCAGTG CCAGGTGTCT
    TCTGACCCCC CAGCCACCAT CATCTGGACG CTGAACGGAA AGACCCTCAA GACCACCAAG
    TTCATCATCC TCTCCCAGGA AGGCTCACTC TGCTCCGTCT CCATCGAGAA GGCACTGCCT
    GAGGACAGAG GCTTATACAA GTGTGTAGCC AAGAATGACG CTGGCCAGGC GGAGTGCTCC
    TGCCAAGTCA CCGTGGATGA TGCTCCAGCC AGTGAGAACA CCAAGGCCCC AGAGATGAAA
    TCCCGGAGGC CCAAGAGCTC TCTTCCTCCC GTGCTAGGAA CTGAGAGTGA TGCGACTGTG
    AAAAAGAAAC CTGCCCCCAA GACACCTCCG AAGGCAGCAA TGCCCCCTCA GATCATCCAG
    TTCCCTGAGG ACCAGAAGGT ACGCGCAGGA GAGTCAGTGG AGCTGTTTGG CAAAGTGACA
    GGCACTCAGC CCATCACCTG TACCTGGATG AAGTTCCGAA AGCAGATCCA GGAAAGCGAG
    CACATGAAGG TGGAGAACAG CGAGAATGGC AGCAAGCTCA CCATCCTGGC CGCGCGCCAG
    GAGCACTGCG GCTGCTACAC ACTGCTGGTG GAGAACAAGC TGGGCAGCAG GCAGGCCCAG
    GTCAACCTCA CTGTCGTGGA TAAGCCAGAC CCCCCAGCTG GCACACCTTG TGCCTCTGAC
    ATTCGGAGCT CCTCACTGAC CCTGTCCTGG TATGGCTCCT CATATGATGG GGGCAGTGCT
    GTACAGTCCT ACAGCATCGA GATCTGGGAC TCAGCCAACA AGACGTGGAA GGAACTAGCC
    ACATGCCGCA GCACCTCTTT CAACGTCCAG GACCTGCTGC CTGACCACGA ATATAAGTTC
    CGTGTACGTG CAATCAACGT GTATGGAACC AGTGAGCCAA GCCAGGAGTC TGAACTCACA
    ACGGTAGGAG AGAAACCTGA AGAGCCGAAG GATGAAGTGG AGGTGTCAGA TGATGATGAG
    AAGGAGCCCG AGGTTGATTA CCGGACAGTG ACAATCAATA CTGAACAAAA AGTATCTGAC
    TTCTACGACA TTGAGGAGAG ATTAGGATCT GGGAAATTTG GACAGGTCTT TCGACTTGTA
    GAAAAGAAAA CTCGAAAAGT CTGGGCAGGG AAGTTCTTCA AGGCATATTC AGCAAAAGAG
    AAAGAGAATA TCCGGCAGGA GATTAGCATC ATGAACTGCC TCCACCACCC TAAGCTGGTC
    CAGTGTGTGG ATGCCTTTGA AGAAAAGGCC AACATCGTCA TGGTCCTGGA GATCGTGTCA
    GGAGGGGAGC TGTTTGAGCG CATCATTGAC GAGGACTTTG AGCTGACGGA GCGTGAGTGC
    ATCAAGTACA TGCGGCAGAT CTCGGAGGGA GTGGAGTACA TCCACAAGCA GGGCATCGTG
    CACCTGGACC TCAAGCCGGA GAACATCATG TGTGTCAACA AGACGGGCAC CAGGATCAAG
    CTCATCGACT TTGGTCTGGC CAGGAGGCTG GAGAATGCGG GGTCTCTGAA GGTCCTCTTT
    GGCACCCCAG AATTTGTGGC TCCTGAAGTG ATCAACTATG AGCCCATCGG CTACGCCACA
    GACATGTGGA GCATCGGGGT CATCTGCTAC ATCCTAGTCA GTGGCCTTTC CCCCTTCATG
    GGAGACAACG ATAACGAAAC CTTGGCCAAC GTTACCTCAG CCACCTGGGA CTTCGACGAC
    GAGGCATTCG ATGAGATCTC CGACGATGCC AAGGATTTCA TCAGCAATCT GCTGAAGAAA
    GATATGAAAA ACCGCCTGGA CTGCACGCAG TGCCTTCAGC ATCCATGGCT AATGAAAGAT
    ACCAAGAACA TGGAGGCCAA GAAACTCTCC AAGGACCGGA TGAAGAAGTA CATGGCAAGA
    AGGAAATGGC AGAAAACGGG CAATGCTGTG AGAGCCATTG GAAGACTGTC CTCTATGGCA
    ATGATCTCAG GGCTCAGTGG CAGGAAATCC TCAACAGGGT CACCAACCAG CCCGCTCAAT
    GCAGAAAAAC TAGAATCTGA AGATGTGTCC CAAGCTTTCC TTGAGGCTGT TGCTGAGGAA
    AAGCCTCATG TAAAACCCTA TTTCTCTAAG ACCATTCGCG ATTTAGAAGT TGTGGAGGGA
    AGTGCTGCTA GATTTGACTG CAAGATTGAA GGATACCCAG ACCCCGAGGT TGTCTGGTTC
    AAAGATGACC AGTCAATCAG GGAGTCCCGC CACTTCCAGA TAGACTACGA TGAGGACGGG
    AACTGCTCTT TAATTATTAG TGATGTTTGC GGGGATGACG ATGCCAAGTA CACCTGCAAG
    GCTGTCAACA GTCTTGGAGA AGCCACCTGC ACAGCAGAGC TCATTGTGGA AACGATGGAG
    GAAGGTGAAG GGGAAGGGGA AGAGGAAGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861958.1
    CDS649..3624
    Translation

    Target ORF information:

    RefSeq Version XM_017006469.2
    Organism Homo sapiens(human)
    Definition Homo sapiens myosin light chain kinase (MYLK), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006469.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATTTCC GTGCCAACCT GCAGCGGCAA GTGAAGCCAA AGACTGTGTC TGAGGAAGAG 
    AGGAAGGTGC ACAGCCCCCA GCAGGTCGAT TTTCGCTCTG TCCTGGCCAA GAAGGGGACT
    TCCAAGACCC CCGTGCCTGA GAAGGTGCCA CCGCCAAAAC CTGCCACCCC GGATTTTCGC
    TCAGTGCTGG GTGGCAAGAA GAAATTACCA GCAGAGAATG GCAGCAGCAG TGCCGAGACC
    CTGAATGCCA AGGCAGTGGA GAGTTCCAAG CCCCTGAGCA ATGCACAGCC TTCAGGGCCC
    TTGAAACCCG TGGGCAACGC CAAGCCTGCT GAGACCCTGA AGCCAATGGG CAACGCCAAG
    CCTGCCGAGA CCCTGAAGCC CATGGGCAAT GCCAAGCCTG ATGAGAACCT GAAATCCGCT
    AGCAAAGAAG AACTCAAGAA AGACGTTAAG AATGATGTGA ACTGCAAGAG AGGCCATGCA
    GGGACCACAG ATAATGAAAA GAGATCAGAG AGCCAGGGGA CAGCCCCAGC CTTCAAGCAG
    AAGCTGCAAG ATGTTCATGT GGCAGAGGGC AAGAAGCTGC TGCTCCAGTG CCAGGTGTCT
    TCTGACCCCC CAGCCACCAT CATCTGGACG CTGAACGGAA AGACCCTCAA GACCACCAAG
    TTCATCATCC TCTCCCAGGA AGGCTCACTC TGCTCCGTCT CCATCGAGAA GGCACTGCCT
    GAGGACAGAG GCTTATACAA GTGTGTAGCC AAGAATGACG CTGGCCAGGC GGAGTGCTCC
    TGCCAAGTCA CCGTGGATGA TGCTCCAGCC AGTGAGAACA CCAAGGCCCC AGAGATGAAA
    TCCCGGAGGC CCAAGAGCTC TCTTCCTCCC GTGCTAGGAA CTGAGAGTGA TGCGACTGTG
    AAAAAGAAAC CTGCCCCCAA GACACCTCCG AAGGCAGCAA TGCCCCCTCA GATCATCCAG
    TTCCCTGAGG ACCAGAAGGT ACGCGCAGGA GAGTCAGTGG AGCTGTTTGG CAAAGTGACA
    GGCACTCAGC CCATCACCTG TACCTGGATG AAGTTCCGAA AGCAGATCCA GGAAAGCGAG
    CACATGAAGG TGGAGAACAG CGAGAATGGC AGCAAGCTCA CCATCCTGGC CGCGCGCCAG
    GAGCACTGCG GCTGCTACAC ACTGCTGGTG GAGAACAAGC TGGGCAGCAG GCAGGCCCAG
    GTCAACCTCA CTGTCGTGGA TAAGCCAGAC CCCCCAGCTG GCACACCTTG TGCCTCTGAC
    ATTCGGAGCT CCTCACTGAC CCTGTCCTGG TATGGCTCCT CATATGATGG GGGCAGTGCT
    GTACAGTCCT ACAGCATCGA GATCTGGGAC TCAGCCAACA AGACGTGGAA GGAACTAGCC
    ACATGCCGCA GCACCTCTTT CAACGTCCAG GACCTGCTGC CTGACCACGA ATATAAGTTC
    CGTGTACGTG CAATCAACGT GTATGGAACC AGTGAGCCAA GCCAGGAGTC TGAACTCACA
    ACGGTAGGAG AGAAACCTGA AGAGCCGAAG GATGAAGTGG AGGTGTCAGA TGATGATGAG
    AAGGAGCCCG AGGTTGATTA CCGGACAGTG ACAATCAATA CTGAACAAAA AGTATCTGAC
    TTCTACGACA TTGAGGAGAG ATTAGGATCT GGGAAATTTG GACAGGTCTT TCGACTTGTA
    GAAAAGAAAA CTCGAAAAGT CTGGGCAGGG AAGTTCTTCA AGGCATATTC AGCAAAAGAG
    AAAGAGAATA TCCGGCAGGA GATTAGCATC ATGAACTGCC TCCACCACCC TAAGCTGGTC
    CAGTGTGTGG ATGCCTTTGA AGAAAAGGCC AACATCGTCA TGGTCCTGGA GATCGTGTCA
    GGAGGGGAGC TGTTTGAGCG CATCATTGAC GAGGACTTTG AGCTGACGGA GCGTGAGTGC
    ATCAAGTACA TGCGGCAGAT CTCGGAGGGA GTGGAGTACA TCCACAAGCA GGGCATCGTG
    CACCTGGACC TCAAGCCGGA GAACATCATG TGTGTCAACA AGACGGGCAC CAGGATCAAG
    CTCATCGACT TTGGTCTGGC CAGGAGGCTG GAGAATGCGG GGTCTCTGAA GGTCCTCTTT
    GGCACCCCAG AATTTGTGGC TCCTGAAGTG ATCAACTATG AGCCCATCGG CTACGCCACA
    GACATGTGGA GCATCGGGGT CATCTGCTAC ATCCTAGTCA GTGGCCTTTC CCCCTTCATG
    GGAGACAACG ATAACGAAAC CTTGGCCAAC GTTACCTCAG CCACCTGGGA CTTCGACGAC
    GAGGCATTCG ATGAGATCTC CGACGATGCC AAGGATTTCA TCAGCAATCT GCTGAAGAAA
    GATATGAAAA ACCGCCTGGA CTGCACGCAG TGCCTTCAGC ATCCATGGCT AATGAAAGAT
    ACCAAGAACA TGGAGGCCAA GAAACTCTCC AAGGACCGGA TGAAGAAGTA CATGGCAAGA
    AGGAAATGGC AGAAAACGGG CAATGCTGTG AGAGCCATTG GAAGACTGTC CTCTATGGCA
    ATGATCTCAG GGCTCAGTGG CAGGAAATCC TCAACAGGGT CACCAACCAG CCCGCTCAAT
    GCAGAAAAAC TAGAATCTGA AGATGTGTCC CAAGCTTTCC TTGAGGCTGT TGCTGAGGAA
    AAGCCTCATG TAAAACCCTA TTTCTCTAAG ACCATTCGCG ATTTAGAAGT TGTGGAGGGA
    AGTGCTGCTA GATTTGACTG CAAGATTGAA GGATACCCAG ACCCCGAGGT TGTCTGGTTC
    AAAGATGACC AGTCAATCAG GGAGTCCCGC CACTTCCAGA TAGACTACGA TGAGGACGGG
    AACTGCTCTT TAATTATTAG TGATGTTTGC GGGGATGACG ATGCCAAGTA CACCTGCAAG
    GCTGTCAACA GTCTTGGAGA AGCCACCTGC ACAGCAGAGC TCATTGTGGA AACGATGGAG
    GAAGGTGAAG GGGAAGGGGA AGAGGAAGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.