MYLK cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYLK
Entrez Gene ID	396445
Full Name	myosin light chain kinase
Synonyms	MLCK
General protein information	Preferred Names myosin light chain kinase Names myosin light chain kinase, smooth muscle kinase related protein myosin, light polypeptide kinase smooth muscle myosin light chain kinase (61-kDa active fragment) telokin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001322361.1	NP_001309290.1	myosin light chain kinase, smooth muscle isoform 1
NM_205459.3	NP_990790.2	myosin light chain kinase, smooth muscle isoform 2
XM_015289794.1	XP_015145280.1	myosin light chain kinase, smooth muscle isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYLK	XP_005639631.1
	MYLK	XP_001113525.1
Gallus gallus (chicken)	MYLK	NP_990790.1
Xenopus tropicalis (tropical clawed frog)	LOC100489678	XP_002935598.2
Homo sapiens (human)	MYLK	NP_444253.3
Pan troglodytes (chimpanzee)	MYLK	XP_003950224.1
Mus musculus (house mouse)	Mylk	NP_647461.3
Rattus norvegicus (Norway rat)	Mylk	NP_001099344.2
Danio rerio (zebrafish)	mylkb	XP_002662355.2

Gene Ontology
Bibliography

Related articles in PubMed

Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates.
Alcala DB, Haldeman BD, Brizendine RK, Krenc AK, Baker JE, Rock RS, Cremo CR
Cell biochemistry and function34(7)469-474(2016 Oct)

Kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system.
Hong F, Facemyer KC, Carter MS, Jackson del R, Haldeman BD, Ruana N, Sutherland C, Walsh MP, Cremo CR, Baker JE
Biochemistry52(47)8489-500(2013 Nov)

Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells.
Thatcher SE, Fultz ME, Tanaka H, Hagiwara H, Zhang HL, Zhang Y, Hayakawa K, Yoshiyama S, Nakamura A, Wang HH, Katayama T, Watanabe M, Lin Y, Wright GL, Kohama K
Journal of pharmacological sciences116(1)116-27(2011)

Characterization of tightly associated smooth muscle myosin-myosin light-chain kinase-calmodulin complexes.
Hong F, Haldeman BD, John OA, Brewer PD, Wu YY, Ni S, Wilson DP, Walsh MP, Baker JE, Cremo CR
Journal of molecular biology390(5)879-92(2009 Jul)

Two distinct modes of myosin assembly and dynamics during epithelial wound closure.
Tamada M, Perez TD, Nelson WJ, Sheetz MP
The Journal of cell biology176(1)27-33(2007 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphorylation of nonmuscle and smooth muscle myosin by myosin light chain kinase (MLCK) is required for activation of myosin's ATPase activity.
Title: Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates.

Data suggest that dissociation of MLCK (myosin light chain kinase) from phosphorylated SMM (smooth muscle myosin) is the rate-limiting step in SMM phosphorylation kinetic cycle in smooth muscle.
Title: Kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system.

Results from various lines of investigation suggest that the actin binding properties of MLCK play important roles in determining actin cytoskeletal organization in smooth muscle, particularly an oligopeptide based on chicken MLCK (26-41).
Title: Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells.

MLCK and calmodulin (CaM) co-purify with unphosphorylated SMM from chicken gizzard, suggesting that they are tightly bound.
Title: Characterization of tightly associated smooth muscle myosin-myosin light-chain kinase-calmodulin complexes.

Tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring.
Title: Two distinct modes of myosin assembly and dynamics during epithelial wound closure.

The following MYLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa38547	XM_015289794.1 Latest version!	Gallus gallus myosin light chain kinase (MYLK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OGa86445	NM_205459.3 Latest version!	Gallus gallus myosin light chain kinase (MYLK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa86450	NM_001322361.1 Latest version!	Gallus gallus myosin light chain kinase (MYLK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYLK ( XM_015289794.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145280.1 Gallus gallus myosin light chain kinase (MYLK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa38547
Clone ID Related Accession (Same CDS sequence)	XM_015289794.1
Accession Version	XM_015289794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	myosin light chain kinase, smooth muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGA TTTCTGGTAT GAGTGGGAGG AAGGCCTCTG GGAGCTCGCC TACCAGCCCG 
ATAAATGCAG ACAAAGTAGA GAACGAAGAT GCCTTCCTTG AAGAAGTGGC TGAGGAAAAG 
CCCCATGTAA AGCCATACTT TACTAAAACA ATCCTTGACA TGGAGGTTGT GGAAGGAAGT 
GCTGCTAGGT TTGACTGCAA AATCGAAGGC TATCCTGACC CTGAGGTAAT GTGGTACAAA 
GATGATCAGC CTGTCAAGGA GTCCCGTCAC TTCCAGATAG ATTATGATGA GGAAGGGAAC 
TGTTCTTTGA CTATTTCTGA AGTATGTGGG GACGATGATG CCAAATACAC CTGCAAAGCT 
GTTAACAGCC TTGGGGAAGC CACATGTACC GCAGAACTCC TTGTGGAAAC AATGGGAAAA 
GAGGGAGAAG GAGAAGGAGA AGGAGAAGAA GATGAAGAGG AGGAGGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145280.1
CDS	61..534
Translation	MAMISGMSGRKASGSSPTSPINADKVENEDAFLEEVAEEKPHVKPYFTKTILDMEVVEGSAARFDCKIEGYPDPEVMWYKDDQPVKESRHFQIDYDEEGNCSLTISEVCGDDDAKYTCKAVNSLGEATCTAELLVETMGKEGEGEGEGEEDEEEEEE

Target ORF information:

RefSeq Version	XM_015289794.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin light chain kinase (MYLK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289794.1

ORF Insert Sequence:

ATGGCAATGA TTTCTGGTAT GAGTGGGAGG AAGGCCTCTG GGAGCTCGCC TACCAGCCCG 
ATAAATGCAG ACAAAGTAGA GAACGAAGAT GCCTTCCTTG AAGAAGTGGC TGAGGAAAAG 
CCCCATGTAA AGCCATACTT TACTAAAACA ATCCTTGACA TGGAGGTTGT GGAAGGAAGT 
GCTGCTAGGT TTGACTGCAA AATCGAAGGC TATCCTGACC CTGAGGTAAT GTGGTACAAA 
GATGATCAGC CTGTCAAGGA GTCCCGTCAC TTCCAGATAG ATTATGATGA GGAAGGGAAC 
TGTTCTTTGA CTATTTCTGA AGTATGTGGG GACGATGATG CCAAATACAC CTGCAAAGCT 
GTTAACAGCC TTGGGGAAGC CACATGTACC GCAGAACTCC TTGTGGAAAC AATGGGAAAA 
GAGGGAGAAG GAGAAGGAGA AGGAGAAGAA GATGAAGAGG AGGAGGAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLK ( NM_205459.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990790.2 Gallus gallus myosin light chain kinase (MYLK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OGa86445
Clone ID Related Accession (Same CDS sequence)	NM_205459.3
Accession Version	NM_205459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Gallus gallus(chicken)
Product	myosin light chain kinase, smooth muscle isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04023946.1. On Apr 12, 2016 this sequence version replaced NM_205459.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGATG TTAAACTGGT GACATCTACT CGAGTATCCA AAACCTCCCT GACACTCAGT 
CCCTCGGTTC CTGCTGAAGC ACCAGCATTT ACTCTTCCTC CTCGGAATAT CCGAGTGCAA 
CTGGGAGCCA CAGCAAGATT TGAGGGGAAG GTCAGAGGCT ACCCTGAGCC ACAGATCACA 
TGGTACAGAA ATGGACATCC TCTCCCAGAA GGAGACCATT ATGTTGTGGA CCACAGCATT 
CGGGGGATAT TCAGCTTGGT GATCAAAGGC GTGCAGGAAG GTGATAGTGG TAAATACACC 
TGTGAGGCTG CCAATGATGG TGGGGTTCGT CAAGTGACTG TGGAATTGAC TGTGGAAGGA 
AATTCCTTGA AGAAATACAG CCTACCATCC AGTGCCAAAA CCCCTGGGGG AAGACTAAGT 
GTTCCACCTG TGGAGCATAG GCCAAGCATC TGGGGAGAGA GTCCACCAAA GTTTGCTACA 
AAACCCAATA GAGTTGTTGT GCGTGAAGGC CAAACAGGCA GATTCTCATG TAAAATAACA 
GGACGACCTC AGCCTCAAGT AACTTGGACT AAGGGTGATA TTCATCTGCA GCAAAATGAG 
CGTTTCAACA TGTTTGAGAA AACAGGAATC CAGTACTTGG AAATCCAAAA CGTTCAGCTT 
GCTGATGCAG GAATTTACAC GTGCACGGTG GTAAACAGTG CTGGGAAAGC ATCTGTGTCT 
GCAGAGCTCA CAGTTCAAGG TCCAGATAAG ACTGACACAC ATGCTCAGCC ACTATGCATG 
CCACCAAAAC CAACAACGCT TGCTACTAAA GCTACTGAAA ACTCAGATTT CAAACAGGCA 
ACTAGCAATG GGATTGTTAC AGAGCTAAAA TCTACCAGCA CTGAACTCAT GGTTGAAACC 
AAAGACAGGC TATCAGCAAA GAAAGAGACC TTTTACACCT CCAGAGAAGC CAAAGATGGT 
AAGCAGGGAC AGAACCAAGA AGCCAATGCA GTACCTCTTC AAGAATCTAG AGGAACCAAG 
GGGCCTCAGG TCTTACAGAA AACTTCAAGC ACCATCACAC TACAAGCTGT CAAAGCACAA 
CCAGAACCAA AGGCAGAACC CCAGACTACT TTCATCAGAC AGGCTGAAGA CAGGAAAAGG 
ACTGTGCAGC CCCTGATGAC AACTACAACT CAAGAAAACC CTTCTCTGAC AGGACAAGTA 
AGTCCAAGGA GTAGAGAAAC AGAAAACAGA GCTGGAGTCC GGAAGTCTGT AAAGGAGGAA 
AAGAGAGAGC CTTTGGGAAT TCCTCCCCAG TTTGAGAGCC GCCCACAGAG CCTGGAAGCA 
TCAGAAGGAC AGGAGATTAA ATTCAAGAGC AAAGTTTCAG GGAAACCAAA ACCAGATGTG 
GAGTGGTTCA AAGAGGGTGT ACCTATTAAA ACTGGAGAAG GTATTCAAAT TTATGAAGAA 
GATGGTACTC ATTGCTTATG GTTAAAGAAA GCCTGCCTGG GGGACAGTGG ATCTTACAGC 
TGTGCAGCTT TCAACCCCAG AGGCCAAACT TCCACCAGCT GGTTGTTAAC TGTCAAAAGG 
CCTAAAGTTG AAGAGGTTGC TCCATGCTTT TCCAGTGTCT TGAAAGGATG CACTGTGAGT 
GAAGGGCAAG ACTTTGTGCT GCAGTGCTAC GTAGGAGGTG TACCTGTGCC TGAGATCACA 
TGGCTTCTCA ATGAACAACC AATTCAGTAT GCCCATTCCA CTTTCGAAGC TGGAGTTGCA 
AAACTGACTG TTCAAGATGC CTTGCCAGAA GATGATGGCA TTTATACATG TCTGGCTGAA 
AACAATGCAG GACGGGCATC ATGCAGTGCT CAGGTCACAG TCAAAGAAAA GAAGAGCAGC 
AAGAAGGCAG AAGGTACACA GGCTGCCAAA TTAAACAAGA CTTTTGCACC CATCTTCCTT 
AAAGGGCTGA CTGACCTCAA AGTAATGGAT GGAAGTCAAG TGATAATGAC TGTGGAAGTA 
TCAGCTAACC CATGTCCTGA AATTATCTGG CTGCACAATG GGAAAGAAAT CCAGGAGACA 
GAAGATTTTC ACTTTGAGAA GAAAGGCAAT GAATACAGTC TGTACATCCA GGAAGTATTT 
CCTGAAGACA CAGGCAAATA CACCTGTGAA GCCTGGAATG AATTAGGAGA AACTCAAACA 
CAGGCTACAT TGACAGTGCA GGAGCCTCAG GATGGCATTC AGCCGTGGTT CATTAGCAAA 
CCGAGATCAG TGACAGCAGC TGCTGGGCAA AATGTCCTTA TATCTTGTGC CATTGCTGGA 
GATCCTTTCC CCACTGTTCA CTGGTTTAAA GATGGGCAAG AAATAACACC AGGAACAGGC 
TGTGAAATCC TGCAAAATGA AGATATCTTC ACACTGATCT TGAGGAATGT ACAGTCTCGT 
CATGCAGGGC AATATGAAAT TCAGCTCAGG AACCAAGTTG GAGAATGCAG CTGCCAGGTG 
TCTCTGATGC TGCGAGAGAG TTCAGCTTCC AGAGCAGAAA TGCTCAGAGA TGGGAGGGAA 
TCAGCCAGCT CTGGAGAGCG AAGAGATGGT GGAAATTATG GTGCACTAAC ATTTGGTAGA 
ACAAGTGGCT TCAAAAAGAG CAGCAGTGAA ACCAGAGCAG CTGAGGAAGA GCAGGAAGAC 
GTCCGAGGCG TACTGAAGAG GCGAGTGGAA ACTCGGGAAC ACACAGAGGA GAGCCTGAGG 
CAGCAGGAAG CTGAACAGCT TGATTTCCGT GACATTTTAG GCAAGAAAGT CAGCACCAAA 
AGTTTTTCTG AGGAGGATCT GAAAGAAATC CCAGCCGAGC AAATGGACTT CCGAGCAAAC 
CTGCAGCGGC AGGTCAAACC CAAGACCTTG TCAGAGGAGG AAAGGAAAGT TCATGCTCCT 
CAGCAGGTGG ACTTCCGCTC CGTGCTGGCC AAGAAAGGCA CCCCAAAGAC CCCACTGCCA 
GAGAAGGTGC CACCTCCTAA GCCAGCCGTT ACAGATTTCA GATCTGTGCT GGGTGCAAAG 
AAGAAGCCCC CTGCAGAGAA TGGCAGCGCT AGCACCCCAG CACCAAATGC CCGTGCCGGT 
TCTGAAGCAC AGAATGCCAC CCCCAATTCT GAAGCCCCTG CTCCCAAACC AGTAGTGAAA 
AAAGAAGAGA AGAATGACAG GAAATGTGAA CATGGGTGTG CAGTGGTGGA TGGAGGAATA 
ATTGGGAAAA AAGCTGAAAA CAAACCAGCA GCAAGCAAGC CAACACCACC ACCAAGCAAA 
GGAACAGCAC CATCCTTTAC AGAGAAGCTT CAGGATGCTA AAGTAGCAGA TGGTGAAAAA 
TTGGTCTTAC AATGTCGAAT TTCCTCTGAT CCCCCTGCAT CTGTCAGCTG GACGCTAGAC 
AGCAAAGCCA TCAAATCATC CAAGTCCATT GTCATCTCCC AAGAAGGAAC ACTGTGTTCA 
CTAACCATTG AGAAGGTCAT GCCAGAAGAT GGAGGAGAAT ACAAATGCAT AGCAGAGAAT 
GCAGCAGGAA AAGCAGAATG TGCTTGCAAA GTGCTTGTGG AAGATACCTC ATCCACAAAG 
GCTGCGAAGC CAGCTGAGAA AAAGACCAAG AAGCCTAAGA CCACACTTCC CCCTGTGCTG 
TCAACAGAAA GCAGTGAAGC AACAGTGAAG AAGAAACCAG CTCCAAAGAC TCCTCCAAAA 
GCAGCTACTC CCCCTCAGAT CACTCAGTTC CCAGAAGACA GAAAAGTCCG TGCTGGTGAA 
TCAGTGGAAT TGTTTGCCAA AGTGGTAGGA ACAGCACCCA TAACTTGCAC CTGGATGAAA 
TTCCGTAAGC AGATTCAAGA AAATGAATAT ATCAAAATTG AGAATGCTGA AAACAGCAGC 
AAGCTAACAA TATCATCGAC AAAGCAGGAA CACTGCGGAT GTTACACGCT AGTTGTAGAA 
AACAAACTTG GCAGCAGACA AGCACAAGTC AACCTTACAG TTGTTGATAA ACCAGACCCA 
CCTGCTGGAA CGCCTTGTGC ATCAGACATA CGGAGTTCTT CCCTCACTCT TTCATGGTAC 
GGCTCCTCTT ATGATGGTGG AAGTGCGGTG CAGTCCTACA CTGTGGAGAT CTGGAACTCA 
GTGGATAACA AATGGACAGA CCTCACTACC TGCCGCAGCA CGTCTTTCAA CGTGCAGGAC 
CTGCAGGCTG ACCGGGAGTA CAAATTCCGT GTACGAGCTG CTAACGTGTA CGGCATCAGT 
GAGCCCAGTC AAGAATCTGA GGTGGTAAAA GTTGGAGAGA AACAAGAAGA AGAACTTAAA 
GAGGAGGAAG CAGAGCTGTC TGACGATGAA GGCAAAGAAA CAGAGGTCAA CTATCGGACA 
GTTACTATCA ACACCGAACA GAAAGTTTCA GATGTCTATA ATATTGAAGA AAGACTGGGA 
TCGGGGAAAT TTGGACAAGT CTTTAGGCTT GTTGAAAAGA AGACTGGAAA AGTGTGGGCA 
GGAAAATTTT TCAAAGCATA TTCTGCAAAG GAAAAAGAGA ACATAAGGGA TGAAATCAGC 
ATCATGAACT GTCTGCATCA CCCAAAGCTC GTGCAATGTG TAGATGCCTT TGAAGAAAAA 
GCCAACATTG TTATGGTCTT GGAAATGGTT TCTGGAGGAG AACTCTTTGA ACGTATCATT 
GATGAAGACT TTGAACTGAC AGAGAGAGAA TGCATTAAAT ATATGAGGCA GATTTCAGAA 
GGAGTTGAGT ACATCCATAA GCAGGGTATT GTCCATTTGG ATTTGAAGCC AGAAAATATT 
ATGTGTGTCA ACAAGACTGG TACAAGTATC AAACTCATTG ACTTCGGACT TGCAAGAAGA 
TTAGAAAGTG CAGGTTCTCT CAAAGTTCTC TTTGGGACAC CTGAGTTTGT GGCTCCAGAA 
GTTATAAACT ATGAACCTAT CGGATATGAA ACAGATATGT GGAGTATAGG AGTTATCTGC 
TACATCCTGG TCAGCGGGCT TTCTCCCTTC ATGGGAGACA ACGACAACGA GACCCTGGCC 
AACGTCACCT CTGCCACGTG GGACTTCGAT GATGAGGCTT TTGATGAAAT CTCTGACGAT 
GCCAAGGACT TTATCAGCAA TCTCCTGAAG AAAGACATGA AGAGTCGCTT AAATTGTACT 
CAATGTCTTC AGCACCCGTG GCTGCAAAAA GACACCAAAA ACATGGAAGC CAAAAAACTT 
TCCAAAGATA GGATGAAGAA ATATATGGCC AGAAGAAAAT GGCAGAAAAC AGGCCATGCT 
GTCCGAGCAA TAGGAAGACT GTCATCCATG GCAATGATTT CTGGTATGAG TGGGAGGAAG 
GCCTCTGGGA GCTCGCCTAC CAGCCCGATA AATGCAGACA AAGTAGAGAA CGAAGATGCC 
TTCCTTGAAG AAGTGGCTGA GGAAAAGCCC CATGTAAAGC CATACTTTAC TAAAACAATC 
CTTGACATGG AGGTTGTGGA AGGAAGTGCT GCTAGGTTTG ACTGCAAAAT CGAAGGCTAT 
CCTGACCCTG AGGTAATGTG GTACAAAGAT GATCAGCCTG TCAAGGAGTC CCGTCACTTC 
CAGATAGATT ATGATGAGGA AGGGAACTGT TCTTTGACTA TTTCTGAAGT ATGTGGGGAC 
GATGATGCCA AATACACCTG CAAAGCTGTT AACAGCCTTG GGGAAGCCAC ATGTACCGCA 
GAACTCCTTG TGGAAACAAT GGGAAAAGAG GGAGAAGGAG AAGGAGAAGG AGAAGAAGAT 
GAAGAGGAGG AGGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990790.2
CDS	113..5833
Translation	MGDVKLVTSTRVSKTSLTLSPSVPAEAPAFTLPPRNIRVQLGATARFEGKVRGYPEPQITWYRNGHPLPEGDHYVVDHSIRGIFSLVIKGVQEGDSGKYTCEAANDGGVRQVTVELTVEGNSLKKYSLPSSAKTPGGRLSVPPVEHRPSIWGESPPKFATKPNRVVVREGQTGRFSCKITGRPQPQVTWTKGDIHLQQNERFNMFEKTGIQYLEIQNVQLADAGIYTCTVVNSAGKASVSAELTVQGPDKTDTHAQPLCMPPKPTTLATKATENSDFKQATSNGIVTELKSTSTELMVETKDRLSAKKETFYTSREAKDGKQGQNQEANAVPLQESRGTKGPQVLQKTSSTITLQAVKAQPEPKAEPQTTFIRQAEDRKRTVQPLMTTTTQENPSLTGQVSPRSRETENRAGVRKSVKEEKREPLGIPPQFESRPQSLEASEGQEIKFKSKVSGKPKPDVEWFKEGVPIKTGEGIQIYEEDGTHCLWLKKACLGDSGSYSCAAFNPRGQTSTSWLLTVKRPKVEEVAPCFSSVLKGCTVSEGQDFVLQCYVGGVPVPEITWLLNEQPIQYAHSTFEAGVAKLTVQDALPEDDGIYTCLAENNAGRASCSAQVTVKEKKSSKKAEGTQAAKLNKTFAPIFLKGLTDLKVMDGSQVIMTVEVSANPCPEIIWLHNGKEIQETEDFHFEKKGNEYSLYIQEVFPEDTGKYTCEAWNELGETQTQATLTVQEPQDGIQPWFISKPRSVTAAAGQNVLISCAIAGDPFPTVHWFKDGQEITPGTGCEILQNEDIFTLILRNVQSRHAGQYEIQLRNQVGECSCQVSLMLRESSASRAEMLRDGRESASSGERRDGGNYGALTFGRTSGFKKSSSETRAAEEEQEDVRGVLKRRVETREHTEESLRQQEAEQLDFRDILGKKVSTKSFSEEDLKEIPAEQMDFRANLQRQVKPKTLSEEERKVHAPQQVDFRSVLAKKGTPKTPLPEKVPPPKPAVTDFRSVLGAKKKPPAENGSASTPAPNARAGSEAQNATPNSEAPAPKPVVKKEEKNDRKCEHGCAVVDGGIIGKKAENKPAASKPTPPPSKGTAPSFTEKLQDAKVADGEKLVLQCRISSDPPASVSWTLDSKAIKSSKSIVISQEGTLCSLTIEKVMPEDGGEYKCIAENAAGKAECACKVLVEDTSSTKAAKPAEKKTKKPKTTLPPVLSTESSEATVKKKPAPKTPPKAATPPQITQFPEDRKVRAGESVELFAKVVGTAPITCTWMKFRKQIQENEYIKIENAENSSKLTISSTKQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYTVEIWNSVDNKWTDLTTCRSTSFNVQDLQADREYKFRVRAANVYGISEPSQESEVVKVGEKQEEELKEEEAELSDDEGKETEVNYRTVTINTEQKVSDVYNIEERLGSGKFGQVFRLVEKKTGKVWAGKFFKAYSAKEKENIRDEISIMNCLHHPKLVQCVDAFEEKANIVMVLEMVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTSIKLIDFGLARRLESAGSLKVLFGTPEFVAPEVINYEPIGYETDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKSRLNCTQCLQHPWLQKDTKNMEAKKLSKDRMKKYMARRKWQKTGHAVRAIGRLSSMAMISGMSGRKASGSSPTSPINADKVENEDAFLEEVAEEKPHVKPYFTKTILDMEVVEGSAARFDCKIEGYPDPEVMWYKDDQPVKESRHFQIDYDEEGNCSLTISEVCGDDDAKYTCKAVNSLGEATCTAELLVETMGKEGEGEGEGEEDEEEEEE

Target ORF information:

RefSeq Version	NM_205459.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin light chain kinase (MYLK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205459.3

ORF Insert Sequence:

ATGGGGGATG TTAAACTGGT GACATCTACT CGAGTATCCA AAACCTCCCT GACACTCAGT 
CCCTCGGTTC CTGCTGAAGC ACCAGCATTT ACTCTTCCTC CTCGGAATAT CCGAGTGCAA 
CTGGGAGCCA CAGCAAGATT TGAGGGGAAG GTCAGAGGCT ACCCTGAGCC ACAGATCACA 
TGGTACAGAA ATGGACATCC TCTCCCAGAA GGAGACCATT ATGTTGTGGA CCACAGCATT 
CGGGGGATAT TCAGCTTGGT GATCAAAGGC GTGCAGGAAG GTGATAGTGG TAAATACACC 
TGTGAGGCTG CCAATGATGG TGGGGTTCGT CAAGTGACTG TGGAATTGAC TGTGGAAGGA 
AATTCCTTGA AGAAATACAG CCTACCATCC AGTGCCAAAA CCCCTGGGGG AAGACTAAGT 
GTTCCACCTG TGGAGCATAG GCCAAGCATC TGGGGAGAGA GTCCACCAAA GTTTGCTACA 
AAACCCAATA GAGTTGTTGT GCGTGAAGGC CAAACAGGCA GATTCTCATG TAAAATAACA 
GGACGACCTC AGCCTCAAGT AACTTGGACT AAGGGTGATA TTCATCTGCA GCAAAATGAG 
CGTTTCAACA TGTTTGAGAA AACAGGAATC CAGTACTTGG AAATCCAAAA CGTTCAGCTT 
GCTGATGCAG GAATTTACAC GTGCACGGTG GTAAACAGTG CTGGGAAAGC ATCTGTGTCT 
GCAGAGCTCA CAGTTCAAGG TCCAGATAAG ACTGACACAC ATGCTCAGCC ACTATGCATG 
CCACCAAAAC CAACAACGCT TGCTACTAAA GCTACTGAAA ACTCAGATTT CAAACAGGCA 
ACTAGCAATG GGATTGTTAC AGAGCTAAAA TCTACCAGCA CTGAACTCAT GGTTGAAACC 
AAAGACAGGC TATCAGCAAA GAAAGAGACC TTTTACACCT CCAGAGAAGC CAAAGATGGT 
AAGCAGGGAC AGAACCAAGA AGCCAATGCA GTACCTCTTC AAGAATCTAG AGGAACCAAG 
GGGCCTCAGG TCTTACAGAA AACTTCAAGC ACCATCACAC TACAAGCTGT CAAAGCACAA 
CCAGAACCAA AGGCAGAACC CCAGACTACT TTCATCAGAC AGGCTGAAGA CAGGAAAAGG 
ACTGTGCAGC CCCTGATGAC AACTACAACT CAAGAAAACC CTTCTCTGAC AGGACAAGTA 
AGTCCAAGGA GTAGAGAAAC AGAAAACAGA GCTGGAGTCC GGAAGTCTGT AAAGGAGGAA 
AAGAGAGAGC CTTTGGGAAT TCCTCCCCAG TTTGAGAGCC GCCCACAGAG CCTGGAAGCA 
TCAGAAGGAC AGGAGATTAA ATTCAAGAGC AAAGTTTCAG GGAAACCAAA ACCAGATGTG 
GAGTGGTTCA AAGAGGGTGT ACCTATTAAA ACTGGAGAAG GTATTCAAAT TTATGAAGAA 
GATGGTACTC ATTGCTTATG GTTAAAGAAA GCCTGCCTGG GGGACAGTGG ATCTTACAGC 
TGTGCAGCTT TCAACCCCAG AGGCCAAACT TCCACCAGCT GGTTGTTAAC TGTCAAAAGG 
CCTAAAGTTG AAGAGGTTGC TCCATGCTTT TCCAGTGTCT TGAAAGGATG CACTGTGAGT 
GAAGGGCAAG ACTTTGTGCT GCAGTGCTAC GTAGGAGGTG TACCTGTGCC TGAGATCACA 
TGGCTTCTCA ATGAACAACC AATTCAGTAT GCCCATTCCA CTTTCGAAGC TGGAGTTGCA 
AAACTGACTG TTCAAGATGC CTTGCCAGAA GATGATGGCA TTTATACATG TCTGGCTGAA 
AACAATGCAG GACGGGCATC ATGCAGTGCT CAGGTCACAG TCAAAGAAAA GAAGAGCAGC 
AAGAAGGCAG AAGGTACACA GGCTGCCAAA TTAAACAAGA CTTTTGCACC CATCTTCCTT 
AAAGGGCTGA CTGACCTCAA AGTAATGGAT GGAAGTCAAG TGATAATGAC TGTGGAAGTA 
TCAGCTAACC CATGTCCTGA AATTATCTGG CTGCACAATG GGAAAGAAAT CCAGGAGACA 
GAAGATTTTC ACTTTGAGAA GAAAGGCAAT GAATACAGTC TGTACATCCA GGAAGTATTT 
CCTGAAGACA CAGGCAAATA CACCTGTGAA GCCTGGAATG AATTAGGAGA AACTCAAACA 
CAGGCTACAT TGACAGTGCA GGAGCCTCAG GATGGCATTC AGCCGTGGTT CATTAGCAAA 
CCGAGATCAG TGACAGCAGC TGCTGGGCAA AATGTCCTTA TATCTTGTGC CATTGCTGGA 
GATCCTTTCC CCACTGTTCA CTGGTTTAAA GATGGGCAAG AAATAACACC AGGAACAGGC 
TGTGAAATCC TGCAAAATGA AGATATCTTC ACACTGATCT TGAGGAATGT ACAGTCTCGT 
CATGCAGGGC AATATGAAAT TCAGCTCAGG AACCAAGTTG GAGAATGCAG CTGCCAGGTG 
TCTCTGATGC TGCGAGAGAG TTCAGCTTCC AGAGCAGAAA TGCTCAGAGA TGGGAGGGAA 
TCAGCCAGCT CTGGAGAGCG AAGAGATGGT GGAAATTATG GTGCACTAAC ATTTGGTAGA 
ACAAGTGGCT TCAAAAAGAG CAGCAGTGAA ACCAGAGCAG CTGAGGAAGA GCAGGAAGAC 
GTCCGAGGCG TACTGAAGAG GCGAGTGGAA ACTCGGGAAC ACACAGAGGA GAGCCTGAGG 
CAGCAGGAAG CTGAACAGCT TGATTTCCGT GACATTTTAG GCAAGAAAGT CAGCACCAAA 
AGTTTTTCTG AGGAGGATCT GAAAGAAATC CCAGCCGAGC AAATGGACTT CCGAGCAAAC 
CTGCAGCGGC AGGTCAAACC CAAGACCTTG TCAGAGGAGG AAAGGAAAGT TCATGCTCCT 
CAGCAGGTGG ACTTCCGCTC CGTGCTGGCC AAGAAAGGCA CCCCAAAGAC CCCACTGCCA 
GAGAAGGTGC CACCTCCTAA GCCAGCCGTT ACAGATTTCA GATCTGTGCT GGGTGCAAAG 
AAGAAGCCCC CTGCAGAGAA TGGCAGCGCT AGCACCCCAG CACCAAATGC CCGTGCCGGT 
TCTGAAGCAC AGAATGCCAC CCCCAATTCT GAAGCCCCTG CTCCCAAACC AGTAGTGAAA 
AAAGAAGAGA AGAATGACAG GAAATGTGAA CATGGGTGTG CAGTGGTGGA TGGAGGAATA 
ATTGGGAAAA AAGCTGAAAA CAAACCAGCA GCAAGCAAGC CAACACCACC ACCAAGCAAA 
GGAACAGCAC CATCCTTTAC AGAGAAGCTT CAGGATGCTA AAGTAGCAGA TGGTGAAAAA 
TTGGTCTTAC AATGTCGAAT TTCCTCTGAT CCCCCTGCAT CTGTCAGCTG GACGCTAGAC 
AGCAAAGCCA TCAAATCATC CAAGTCCATT GTCATCTCCC AAGAAGGAAC ACTGTGTTCA 
CTAACCATTG AGAAGGTCAT GCCAGAAGAT GGAGGAGAAT ACAAATGCAT AGCAGAGAAT 
GCAGCAGGAA AAGCAGAATG TGCTTGCAAA GTGCTTGTGG AAGATACCTC ATCCACAAAG 
GCTGCGAAGC CAGCTGAGAA AAAGACCAAG AAGCCTAAGA CCACACTTCC CCCTGTGCTG 
TCAACAGAAA GCAGTGAAGC AACAGTGAAG AAGAAACCAG CTCCAAAGAC TCCTCCAAAA 
GCAGCTACTC CCCCTCAGAT CACTCAGTTC CCAGAAGACA GAAAAGTCCG TGCTGGTGAA 
TCAGTGGAAT TGTTTGCCAA AGTGGTAGGA ACAGCACCCA TAACTTGCAC CTGGATGAAA 
TTCCGTAAGC AGATTCAAGA AAATGAATAT ATCAAAATTG AGAATGCTGA AAACAGCAGC 
AAGCTAACAA TATCATCGAC AAAGCAGGAA CACTGCGGAT GTTACACGCT AGTTGTAGAA 
AACAAACTTG GCAGCAGACA AGCACAAGTC AACCTTACAG TTGTTGATAA ACCAGACCCA 
CCTGCTGGAA CGCCTTGTGC ATCAGACATA CGGAGTTCTT CCCTCACTCT TTCATGGTAC 
GGCTCCTCTT ATGATGGTGG AAGTGCGGTG CAGTCCTACA CTGTGGAGAT CTGGAACTCA 
GTGGATAACA AATGGACAGA CCTCACTACC TGCCGCAGCA CGTCTTTCAA CGTGCAGGAC 
CTGCAGGCTG ACCGGGAGTA CAAATTCCGT GTACGAGCTG CTAACGTGTA CGGCATCAGT 
GAGCCCAGTC AAGAATCTGA GGTGGTAAAA GTTGGAGAGA AACAAGAAGA AGAACTTAAA 
GAGGAGGAAG CAGAGCTGTC TGACGATGAA GGCAAAGAAA CAGAGGTCAA CTATCGGACA 
GTTACTATCA ACACCGAACA GAAAGTTTCA GATGTCTATA ATATTGAAGA AAGACTGGGA 
TCGGGGAAAT TTGGACAAGT CTTTAGGCTT GTTGAAAAGA AGACTGGAAA AGTGTGGGCA 
GGAAAATTTT TCAAAGCATA TTCTGCAAAG GAAAAAGAGA ACATAAGGGA TGAAATCAGC 
ATCATGAACT GTCTGCATCA CCCAAAGCTC GTGCAATGTG TAGATGCCTT TGAAGAAAAA 
GCCAACATTG TTATGGTCTT GGAAATGGTT TCTGGAGGAG AACTCTTTGA ACGTATCATT 
GATGAAGACT TTGAACTGAC AGAGAGAGAA TGCATTAAAT ATATGAGGCA GATTTCAGAA 
GGAGTTGAGT ACATCCATAA GCAGGGTATT GTCCATTTGG ATTTGAAGCC AGAAAATATT 
ATGTGTGTCA ACAAGACTGG TACAAGTATC AAACTCATTG ACTTCGGACT TGCAAGAAGA 
TTAGAAAGTG CAGGTTCTCT CAAAGTTCTC TTTGGGACAC CTGAGTTTGT GGCTCCAGAA 
GTTATAAACT ATGAACCTAT CGGATATGAA ACAGATATGT GGAGTATAGG AGTTATCTGC 
TACATCCTGG TCAGCGGGCT TTCTCCCTTC ATGGGAGACA ACGACAACGA GACCCTGGCC 
AACGTCACCT CTGCCACGTG GGACTTCGAT GATGAGGCTT TTGATGAAAT CTCTGACGAT 
GCCAAGGACT TTATCAGCAA TCTCCTGAAG AAAGACATGA AGAGTCGCTT AAATTGTACT 
CAATGTCTTC AGCACCCGTG GCTGCAAAAA GACACCAAAA ACATGGAAGC CAAAAAACTT 
TCCAAAGATA GGATGAAGAA ATATATGGCC AGAAGAAAAT GGCAGAAAAC AGGCCATGCT 
GTCCGAGCAA TAGGAAGACT GTCATCCATG GCAATGATTT CTGGTATGAG TGGGAGGAAG 
GCCTCTGGGA GCTCGCCTAC CAGCCCGATA AATGCAGACA AAGTAGAGAA CGAAGATGCC 
TTCCTTGAAG AAGTGGCTGA GGAAAAGCCC CATGTAAAGC CATACTTTAC TAAAACAATC 
CTTGACATGG AGGTTGTGGA AGGAAGTGCT GCTAGGTTTG ACTGCAAAAT CGAAGGCTAT 
CCTGACCCTG AGGTAATGTG GTACAAAGAT GATCAGCCTG TCAAGGAGTC CCGTCACTTC 
CAGATAGATT ATGATGAGGA AGGGAACTGT TCTTTGACTA TTTCTGAAGT ATGTGGGGAC 
GATGATGCCA AATACACCTG CAAAGCTGTT AACAGCCTTG GGGAAGCCAC ATGTACCGCA 
GAACTCCTTG TGGAAACAAT GGGAAAAGAG GGAGAAGGAG AAGGAGAAGG AGAAGAAGAT 
GAAGAGGAGG AGGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLK ( NM_001322361.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001309290.1 Gallus gallus myosin light chain kinase (MYLK), transcript variant 1, mRNA.

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CloneID	OGa86450
Clone ID Related Accession (Same CDS sequence)	NM_001322361.1
Accession Version	NM_001322361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Gallus gallus(chicken)
Product	myosin light chain kinase, smooth muscle isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04023946.1. On Apr 12, 2016 this sequence version replaced XM_015289793.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGGCT TGGAGCGTCG GAGGCGGGAG TTCCCCGCAG CTCTGTCGGA CCCGCTACCG 
AACCGTCCCC TGCGGGAGCG GCCGCGGGGC TGCGACGGGG AGAGGCGCTC ACCTCCAGCT 
CCGCTCCCGG AGTGCGCCGA GCCGAGAGCG AGCGGACCGC GGGTCCACAT CATGGGGGAT 
GTTAAACTGG TGACATCTAC TCGAGTATCC AAAACCTCCC TGACACTCAG TCCCTCGGTT 
CCTGCTGAAG CACCAGCATT TACTCTTCCT CCTCGGAATA TCCGAGTGCA ACTGGGAGCC 
ACAGCAAGAT TTGAGGGGAA GGTCAGAGGC TACCCTGAGC CACAGATCAC ATGGTACAGA 
AATGGACATC CTCTCCCAGA AGGAGACCAT TATGTTGTGG ACCACAGCAT TCGGGGGATA 
TTCAGCTTGG TGATCAAAGG CGTGCAGGAA GGTGATAGTG GTAAATACAC CTGTGAGGCT 
GCCAATGATG GTGGGGTTCG TCAAGTGACT GTGGAATTGA CTGTGGAAGG AAATTCCTTG 
AAGAAATACA GCCTACCATC CAGTGCCAAA ACCCCTGGGG GAAGACTAAG TGTTCCACCT 
GTGGAGCATA GGCCAAGCAT CTGGGGAGAG AGTCCACCAA AGTTTGCTAC AAAACCCAAT 
AGAGTTGTTG TGCGTGAAGG CCAAACAGGC AGATTCTCAT GTAAAATAAC AGGACGACCT 
CAGCCTCAAG TAACTTGGAC TAAGGGTGAT ATTCATCTGC AGCAAAATGA GCGTTTCAAC 
ATGTTTGAGA AAACAGGAAT CCAGTACTTG GAAATCCAAA ACGTTCAGCT TGCTGATGCA 
GGAATTTACA CGTGCACGGT GGTAAACAGT GCTGGGAAAG CATCTGTGTC TGCAGAGCTC 
ACAGTTCAAG GTCCAGATAA GACTGACACA CATGCTCAGC CACTATGCAT GCCACCAAAA 
CCAACAACGC TTGCTACTAA AGCTACTGAA AACTCAGATT TCAAACAGGC AACTAGCAAT 
GGGATTGTTA CAGAGCTAAA ATCTACCAGC ACTGAACTCA TGGTTGAAAC CAAAGACAGG 
CTATCAGCAA AGAAAGAGAC CTTTTACACC TCCAGAGAAG CCAAAGATGG TAAGCAGGGA 
CAGAACCAAG AAGCCAATGC AGTACCTCTT CAAGAATCTA GAGGAACCAA GGGGCCTCAG 
GTCTTACAGA AAACTTCAAG CACCATCACA CTACAAGCTG TCAAAGCACA ACCAGAACCA 
AAGGCAGAAC CCCAGACTAC TTTCATCAGA CAGGCTGAAG ACAGGAAAAG GACTGTGCAG 
CCCCTGATGA CAACTACAAC TCAAGAAAAC CCTTCTCTGA CAGGACAAGT AAGTCCAAGG 
AGTAGAGAAA CAGAAAACAG AGCTGGAGTC CGGAAGTCTG TAAAGGAGGA AAAGAGAGAG 
CCTTTGGGAA TTCCTCCCCA GTTTGAGAGC CGCCCACAGA GCCTGGAAGC ATCAGAAGGA 
CAGGAGATTA AATTCAAGAG CAAAGTTTCA GGGAAACCAA AACCAGATGT GGAGTGGTTC 
AAAGAGGGTG TACCTATTAA AACTGGAGAA GGTATTCAAA TTTATGAAGA AGATGGTACT 
CATTGCTTAT GGTTAAAGAA AGCCTGCCTG GGGGACAGTG GATCTTACAG CTGTGCAGCT 
TTCAACCCCA GAGGCCAAAC TTCCACCAGC TGGTTGTTAA CTGTCAAAAG GCCTAAAGTT 
GAAGAGGTTG CTCCATGCTT TTCCAGTGTC TTGAAAGGAT GCACTGTGAG TGAAGGGCAA 
GACTTTGTGC TGCAGTGCTA CGTAGGAGGT GTACCTGTGC CTGAGATCAC ATGGCTTCTC 
AATGAACAAC CAATTCAGTA TGCCCATTCC ACTTTCGAAG CTGGAGTTGC AAAACTGACT 
GTTCAAGATG CCTTGCCAGA AGATGATGGC ATTTATACAT GTCTGGCTGA AAACAATGCA 
GGACGGGCAT CATGCAGTGC TCAGGTCACA GTCAAAGAAA AGAAGAGCAG CAAGAAGGCA 
GAAGGTACAC AGGCTGCCAA ATTAAACAAG ACTTTTGCAC CCATCTTCCT TAAAGGGCTG 
ACTGACCTCA AAGTAATGGA TGGAAGTCAA GTGATAATGA CTGTGGAAGT ATCAGCTAAC 
CCATGTCCTG AAATTATCTG GCTGCACAAT GGGAAAGAAA TCCAGGAGAC AGAAGATTTT 
CACTTTGAGA AGAAAGGCAA TGAATACAGT CTGTACATCC AGGAAGTATT TCCTGAAGAC 
ACAGGCAAAT ACACCTGTGA AGCCTGGAAT GAATTAGGAG AAACTCAAAC ACAGGCTACA 
TTGACAGTGC AGGAGCCTCA GGATGGCATT CAGCCGTGGT TCATTAGCAA ACCGAGATCA 
GTGACAGCAG CTGCTGGGCA AAATGTCCTT ATATCTTGTG CCATTGCTGG AGATCCTTTC 
CCCACTGTTC ACTGGTTTAA AGATGGGCAA GAAATAACAC CAGGAACAGG CTGTGAAATC 
CTGCAAAATG AAGATATCTT CACACTGATC TTGAGGAATG TACAGTCTCG TCATGCAGGG 
CAATATGAAA TTCAGCTCAG GAACCAAGTT GGAGAATGCA GCTGCCAGGT GTCTCTGATG 
CTGCGAGAGA GTTCAGCTTC CAGAGCAGAA ATGCTCAGAG ATGGGAGGGA ATCAGCCAGC 
TCTGGAGAGC GAAGAGATGG TGGAAATTAT GGTGCACTAA CATTTGGTAG AACAAGTGGC 
TTCAAAAAGA GCAGCAGTGA AACCAGAGCA GCTGAGGAAG AGCAGGAAGA CGTCCGAGGC 
GTACTGAAGA GGCGAGTGGA AACTCGGGAA CACACAGAGG AGAGCCTGAG GCAGCAGGAA 
GCTGAACAGC TTGATTTCCG TGACATTTTA GGCAAGAAAG TCAGCACCAA AAGTTTTTCT 
GAGGAGGATC TGAAAGAAAT CCCAGCCGAG CAAATGGACT TCCGAGCAAA CCTGCAGCGG 
CAGGTCAAAC CCAAGACCTT GTCAGAGGAG GAAAGGAAAG TTCATGCTCC TCAGCAGGTG 
GACTTCCGCT CCGTGCTGGC CAAGAAAGGC ACCCCAAAGA CCCCACTGCC AGAGAAGGTG 
CCACCTCCTA AGCCAGCCGT TACAGATTTC AGATCTGTGC TGGGTGCAAA GAAGAAGCCC 
CCTGCAGAGA ATGGCAGCGC TAGCACCCCA GCACCAAATG CCCGTGCCGG TTCTGAAGCA 
CAGAATGCCA CCCCCAATTC TGAAGCCCCT GCTCCCAAAC CAGTAGTGAA AAAAGAAGAG 
AAGAATGACA GGAAATGTGA ACATGGGTGT GCAGTGGTGG ATGGAGGAAT AATTGGGAAA 
AAAGCTGAAA ACAAACCAGC AGCAAGCAAG CCAACACCAC CACCAAGCAA AGGAACAGCA 
CCATCCTTTA CAGAGAAGCT TCAGGATGCT AAAGTAGCAG ATGGTGAAAA ATTGGTCTTA 
CAATGTCGAA TTTCCTCTGA TCCCCCTGCA TCTGTCAGCT GGACGCTAGA CAGCAAAGCC 
ATCAAATCAT CCAAGTCCAT TGTCATCTCC CAAGAAGGAA CACTGTGTTC ACTAACCATT 
GAGAAGGTCA TGCCAGAAGA TGGAGGAGAA TACAAATGCA TAGCAGAGAA TGCAGCAGGA 
AAAGCAGAAT GTGCTTGCAA AGTGCTTGTG GAAGATACCT CATCCACAAA GGCTGCGAAG 
CCAGCTGAGA AAAAGACCAA GAAGCCTAAG ACCACACTTC CCCCTGTGCT GTCAACAGAA 
AGCAGTGAAG CAACAGTGAA GAAGAAACCA GCTCCAAAGA CTCCTCCAAA AGCAGCTACT 
CCCCCTCAGA TCACTCAGTT CCCAGAAGAC AGAAAAGTCC GTGCTGGTGA ATCAGTGGAA 
TTGTTTGCCA AAGTGGTAGG AACAGCACCC ATAACTTGCA CCTGGATGAA ATTCCGTAAG 
CAGATTCAAG AAAATGAATA TATCAAAATT GAGAATGCTG AAAACAGCAG CAAGCTAACA 
ATATCATCGA CAAAGCAGGA ACACTGCGGA TGTTACACGC TAGTTGTAGA AAACAAACTT 
GGCAGCAGAC AAGCACAAGT CAACCTTACA GTTGTTGATA AACCAGACCC ACCTGCTGGA 
ACGCCTTGTG CATCAGACAT ACGGAGTTCT TCCCTCACTC TTTCATGGTA CGGCTCCTCT 
TATGATGGTG GAAGTGCGGT GCAGTCCTAC ACTGTGGAGA TCTGGAACTC AGTGGATAAC 
AAATGGACAG ACCTCACTAC CTGCCGCAGC ACGTCTTTCA ACGTGCAGGA CCTGCAGGCT 
GACCGGGAGT ACAAATTCCG TGTACGAGCT GCTAACGTGT ACGGCATCAG TGAGCCCAGT 
CAAGAATCTG AGGTGGTAAA AGTTGGAGAG AAACAAGAAG AAGAACTTAA AGAGGAGGAA 
GCAGAGCTGT CTGACGATGA AGGCAAAGAA ACAGAGGTCA ACTATCGGAC AGTTACTATC 
AACACCGAAC AGAAAGTTTC AGATGTCTAT AATATTGAAG AAAGACTGGG ATCGGGGAAA 
TTTGGACAAG TCTTTAGGCT TGTTGAAAAG AAGACTGGAA AAGTGTGGGC AGGAAAATTT 
TTCAAAGCAT ATTCTGCAAA GGAAAAAGAG AACATAAGGG ATGAAATCAG CATCATGAAC 
TGTCTGCATC ACCCAAAGCT CGTGCAATGT GTAGATGCCT TTGAAGAAAA AGCCAACATT 
GTTATGGTCT TGGAAATGGT TTCTGGAGGA GAACTCTTTG AACGTATCAT TGATGAAGAC 
TTTGAACTGA CAGAGAGAGA ATGCATTAAA TATATGAGGC AGATTTCAGA AGGAGTTGAG 
TACATCCATA AGCAGGGTAT TGTCCATTTG GATTTGAAGC CAGAAAATAT TATGTGTGTC 
AACAAGACTG GTACAAGTAT CAAACTCATT GACTTCGGAC TTGCAAGAAG ATTAGAAAGT 
GCAGGTTCTC TCAAAGTTCT CTTTGGGACA CCTGAGTTTG TGGCTCCAGA AGTTATAAAC 
TATGAACCTA TCGGATATGA AACAGATATG TGGAGTATAG GAGTTATCTG CTACATCCTG 
GTCAGCGGGC TTTCTCCCTT CATGGGAGAC AACGACAACG AGACCCTGGC CAACGTCACC 
TCTGCCACGT GGGACTTCGA TGATGAGGCT TTTGATGAAA TCTCTGACGA TGCCAAGGAC 
TTTATCAGCA ATCTCCTGAA GAAAGACATG AAGAGTCGCT TAAATTGTAC TCAATGTCTT 
CAGCACCCGT GGCTGCAAAA AGACACCAAA AACATGGAAG CCAAAAAACT TTCCAAAGAT 
AGGATGAAGA AATATATGGC CAGAAGAAAA TGGCAGAAAA CAGGCCATGC TGTCCGAGCA 
ATAGGAAGAC TGTCATCCAT GGCAATGATT TCTGGTATGA GTGGGAGGAA GGCCTCTGGG 
AGCTCGCCTA CCAGCCCGAT AAATGCAGAC AAAGTAGAGA ACGAAGATGC CTTCCTTGAA 
GAAGTGGCTG AGGAAAAGCC CCATGTAAAG CCATACTTTA CTAAAACAAT CCTTGACATG 
GAGGTTGTGG AAGGAAGTGC TGCTAGGTTT GACTGCAAAA TCGAAGGCTA TCCTGACCCT 
GAGGTAATGT GGTACAAAGA TGATCAGCCT GTCAAGGAGT CCCGTCACTT CCAGATAGAT 
TATGATGAGG AAGGGAACTG TTCTTTGACT ATTTCTGAAG TATGTGGGGA CGATGATGCC 
AAATACACCT GCAAAGCTGT TAACAGCCTT GGGGAAGCCA CATGTACCGC AGAACTCCTT 
GTGGAAACAA TGGGAAAAGA GGGAGAAGGA GAAGGAGAAG GAGAAGAAGA TGAAGAGGAG 
GAGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309290.1
CDS	83..5974
Translation	MPGLERRRREFPAALSDPLPNRPLRERPRGCDGERRSPPAPLPECAEPRASGPRVHIMGDVKLVTSTRVSKTSLTLSPSVPAEAPAFTLPPRNIRVQLGATARFEGKVRGYPEPQITWYRNGHPLPEGDHYVVDHSIRGIFSLVIKGVQEGDSGKYTCEAANDGGVRQVTVELTVEGNSLKKYSLPSSAKTPGGRLSVPPVEHRPSIWGESPPKFATKPNRVVVREGQTGRFSCKITGRPQPQVTWTKGDIHLQQNERFNMFEKTGIQYLEIQNVQLADAGIYTCTVVNSAGKASVSAELTVQGPDKTDTHAQPLCMPPKPTTLATKATENSDFKQATSNGIVTELKSTSTELMVETKDRLSAKKETFYTSREAKDGKQGQNQEANAVPLQESRGTKGPQVLQKTSSTITLQAVKAQPEPKAEPQTTFIRQAEDRKRTVQPLMTTTTQENPSLTGQVSPRSRETENRAGVRKSVKEEKREPLGIPPQFESRPQSLEASEGQEIKFKSKVSGKPKPDVEWFKEGVPIKTGEGIQIYEEDGTHCLWLKKACLGDSGSYSCAAFNPRGQTSTSWLLTVKRPKVEEVAPCFSSVLKGCTVSEGQDFVLQCYVGGVPVPEITWLLNEQPIQYAHSTFEAGVAKLTVQDALPEDDGIYTCLAENNAGRASCSAQVTVKEKKSSKKAEGTQAAKLNKTFAPIFLKGLTDLKVMDGSQVIMTVEVSANPCPEIIWLHNGKEIQETEDFHFEKKGNEYSLYIQEVFPEDTGKYTCEAWNELGETQTQATLTVQEPQDGIQPWFISKPRSVTAAAGQNVLISCAIAGDPFPTVHWFKDGQEITPGTGCEILQNEDIFTLILRNVQSRHAGQYEIQLRNQVGECSCQVSLMLRESSASRAEMLRDGRESASSGERRDGGNYGALTFGRTSGFKKSSSETRAAEEEQEDVRGVLKRRVETREHTEESLRQQEAEQLDFRDILGKKVSTKSFSEEDLKEIPAEQMDFRANLQRQVKPKTLSEEERKVHAPQQVDFRSVLAKKGTPKTPLPEKVPPPKPAVTDFRSVLGAKKKPPAENGSASTPAPNARAGSEAQNATPNSEAPAPKPVVKKEEKNDRKCEHGCAVVDGGIIGKKAENKPAASKPTPPPSKGTAPSFTEKLQDAKVADGEKLVLQCRISSDPPASVSWTLDSKAIKSSKSIVISQEGTLCSLTIEKVMPEDGGEYKCIAENAAGKAECACKVLVEDTSSTKAAKPAEKKTKKPKTTLPPVLSTESSEATVKKKPAPKTPPKAATPPQITQFPEDRKVRAGESVELFAKVVGTAPITCTWMKFRKQIQENEYIKIENAENSSKLTISSTKQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYTVEIWNSVDNKWTDLTTCRSTSFNVQDLQADREYKFRVRAANVYGISEPSQESEVVKVGEKQEEELKEEEAELSDDEGKETEVNYRTVTINTEQKVSDVYNIEERLGSGKFGQVFRLVEKKTGKVWAGKFFKAYSAKEKENIRDEISIMNCLHHPKLVQCVDAFEEKANIVMVLEMVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTSIKLIDFGLARRLESAGSLKVLFGTPEFVAPEVINYEPIGYETDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKSRLNCTQCLQHPWLQKDTKNMEAKKLSKDRMKKYMARRKWQKTGHAVRAIGRLSSMAMISGMSGRKASGSSPTSPINADKVENEDAFLEEVAEEKPHVKPYFTKTILDMEVVEGSAARFDCKIEGYPDPEVMWYKDDQPVKESRHFQIDYDEEGNCSLTISEVCGDDDAKYTCKAVNSLGEATCTAELLVETMGKEGEGEGEGEEDEEEEEE

Target ORF information:

RefSeq Version	NM_001322361.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin light chain kinase (MYLK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322361.1

ORF Insert Sequence:

ATGCCGGGCT TGGAGCGTCG GAGGCGGGAG TTCCCCGCAG CTCTGTCGGA CCCGCTACCG 
AACCGTCCCC TGCGGGAGCG GCCGCGGGGC TGCGACGGGG AGAGGCGCTC ACCTCCAGCT 
CCGCTCCCGG AGTGCGCCGA GCCGAGAGCG AGCGGACCGC GGGTCCACAT CATGGGGGAT 
GTTAAACTGG TGACATCTAC TCGAGTATCC AAAACCTCCC TGACACTCAG TCCCTCGGTT 
CCTGCTGAAG CACCAGCATT TACTCTTCCT CCTCGGAATA TCCGAGTGCA ACTGGGAGCC 
ACAGCAAGAT TTGAGGGGAA GGTCAGAGGC TACCCTGAGC CACAGATCAC ATGGTACAGA 
AATGGACATC CTCTCCCAGA AGGAGACCAT TATGTTGTGG ACCACAGCAT TCGGGGGATA 
TTCAGCTTGG TGATCAAAGG CGTGCAGGAA GGTGATAGTG GTAAATACAC CTGTGAGGCT 
GCCAATGATG GTGGGGTTCG TCAAGTGACT GTGGAATTGA CTGTGGAAGG AAATTCCTTG 
AAGAAATACA GCCTACCATC CAGTGCCAAA ACCCCTGGGG GAAGACTAAG TGTTCCACCT 
GTGGAGCATA GGCCAAGCAT CTGGGGAGAG AGTCCACCAA AGTTTGCTAC AAAACCCAAT 
AGAGTTGTTG TGCGTGAAGG CCAAACAGGC AGATTCTCAT GTAAAATAAC AGGACGACCT 
CAGCCTCAAG TAACTTGGAC TAAGGGTGAT ATTCATCTGC AGCAAAATGA GCGTTTCAAC 
ATGTTTGAGA AAACAGGAAT CCAGTACTTG GAAATCCAAA ACGTTCAGCT TGCTGATGCA 
GGAATTTACA CGTGCACGGT GGTAAACAGT GCTGGGAAAG CATCTGTGTC TGCAGAGCTC 
ACAGTTCAAG GTCCAGATAA GACTGACACA CATGCTCAGC CACTATGCAT GCCACCAAAA 
CCAACAACGC TTGCTACTAA AGCTACTGAA AACTCAGATT TCAAACAGGC AACTAGCAAT 
GGGATTGTTA CAGAGCTAAA ATCTACCAGC ACTGAACTCA TGGTTGAAAC CAAAGACAGG 
CTATCAGCAA AGAAAGAGAC CTTTTACACC TCCAGAGAAG CCAAAGATGG TAAGCAGGGA 
CAGAACCAAG AAGCCAATGC AGTACCTCTT CAAGAATCTA GAGGAACCAA GGGGCCTCAG 
GTCTTACAGA AAACTTCAAG CACCATCACA CTACAAGCTG TCAAAGCACA ACCAGAACCA 
AAGGCAGAAC CCCAGACTAC TTTCATCAGA CAGGCTGAAG ACAGGAAAAG GACTGTGCAG 
CCCCTGATGA CAACTACAAC TCAAGAAAAC CCTTCTCTGA CAGGACAAGT AAGTCCAAGG 
AGTAGAGAAA CAGAAAACAG AGCTGGAGTC CGGAAGTCTG TAAAGGAGGA AAAGAGAGAG 
CCTTTGGGAA TTCCTCCCCA GTTTGAGAGC CGCCCACAGA GCCTGGAAGC ATCAGAAGGA 
CAGGAGATTA AATTCAAGAG CAAAGTTTCA GGGAAACCAA AACCAGATGT GGAGTGGTTC 
AAAGAGGGTG TACCTATTAA AACTGGAGAA GGTATTCAAA TTTATGAAGA AGATGGTACT 
CATTGCTTAT GGTTAAAGAA AGCCTGCCTG GGGGACAGTG GATCTTACAG CTGTGCAGCT 
TTCAACCCCA GAGGCCAAAC TTCCACCAGC TGGTTGTTAA CTGTCAAAAG GCCTAAAGTT 
GAAGAGGTTG CTCCATGCTT TTCCAGTGTC TTGAAAGGAT GCACTGTGAG TGAAGGGCAA 
GACTTTGTGC TGCAGTGCTA CGTAGGAGGT GTACCTGTGC CTGAGATCAC ATGGCTTCTC 
AATGAACAAC CAATTCAGTA TGCCCATTCC ACTTTCGAAG CTGGAGTTGC AAAACTGACT 
GTTCAAGATG CCTTGCCAGA AGATGATGGC ATTTATACAT GTCTGGCTGA AAACAATGCA 
GGACGGGCAT CATGCAGTGC TCAGGTCACA GTCAAAGAAA AGAAGAGCAG CAAGAAGGCA 
GAAGGTACAC AGGCTGCCAA ATTAAACAAG ACTTTTGCAC CCATCTTCCT TAAAGGGCTG 
ACTGACCTCA AAGTAATGGA TGGAAGTCAA GTGATAATGA CTGTGGAAGT ATCAGCTAAC 
CCATGTCCTG AAATTATCTG GCTGCACAAT GGGAAAGAAA TCCAGGAGAC AGAAGATTTT 
CACTTTGAGA AGAAAGGCAA TGAATACAGT CTGTACATCC AGGAAGTATT TCCTGAAGAC 
ACAGGCAAAT ACACCTGTGA AGCCTGGAAT GAATTAGGAG AAACTCAAAC ACAGGCTACA 
TTGACAGTGC AGGAGCCTCA GGATGGCATT CAGCCGTGGT TCATTAGCAA ACCGAGATCA 
GTGACAGCAG CTGCTGGGCA AAATGTCCTT ATATCTTGTG CCATTGCTGG AGATCCTTTC 
CCCACTGTTC ACTGGTTTAA AGATGGGCAA GAAATAACAC CAGGAACAGG CTGTGAAATC 
CTGCAAAATG AAGATATCTT CACACTGATC TTGAGGAATG TACAGTCTCG TCATGCAGGG 
CAATATGAAA TTCAGCTCAG GAACCAAGTT GGAGAATGCA GCTGCCAGGT GTCTCTGATG 
CTGCGAGAGA GTTCAGCTTC CAGAGCAGAA ATGCTCAGAG ATGGGAGGGA ATCAGCCAGC 
TCTGGAGAGC GAAGAGATGG TGGAAATTAT GGTGCACTAA CATTTGGTAG AACAAGTGGC 
TTCAAAAAGA GCAGCAGTGA AACCAGAGCA GCTGAGGAAG AGCAGGAAGA CGTCCGAGGC 
GTACTGAAGA GGCGAGTGGA AACTCGGGAA CACACAGAGG AGAGCCTGAG GCAGCAGGAA 
GCTGAACAGC TTGATTTCCG TGACATTTTA GGCAAGAAAG TCAGCACCAA AAGTTTTTCT 
GAGGAGGATC TGAAAGAAAT CCCAGCCGAG CAAATGGACT TCCGAGCAAA CCTGCAGCGG 
CAGGTCAAAC CCAAGACCTT GTCAGAGGAG GAAAGGAAAG TTCATGCTCC TCAGCAGGTG 
GACTTCCGCT CCGTGCTGGC CAAGAAAGGC ACCCCAAAGA CCCCACTGCC AGAGAAGGTG 
CCACCTCCTA AGCCAGCCGT TACAGATTTC AGATCTGTGC TGGGTGCAAA GAAGAAGCCC 
CCTGCAGAGA ATGGCAGCGC TAGCACCCCA GCACCAAATG CCCGTGCCGG TTCTGAAGCA 
CAGAATGCCA CCCCCAATTC TGAAGCCCCT GCTCCCAAAC CAGTAGTGAA AAAAGAAGAG 
AAGAATGACA GGAAATGTGA ACATGGGTGT GCAGTGGTGG ATGGAGGAAT AATTGGGAAA 
AAAGCTGAAA ACAAACCAGC AGCAAGCAAG CCAACACCAC CACCAAGCAA AGGAACAGCA 
CCATCCTTTA CAGAGAAGCT TCAGGATGCT AAAGTAGCAG ATGGTGAAAA ATTGGTCTTA 
CAATGTCGAA TTTCCTCTGA TCCCCCTGCA TCTGTCAGCT GGACGCTAGA CAGCAAAGCC 
ATCAAATCAT CCAAGTCCAT TGTCATCTCC CAAGAAGGAA CACTGTGTTC ACTAACCATT 
GAGAAGGTCA TGCCAGAAGA TGGAGGAGAA TACAAATGCA TAGCAGAGAA TGCAGCAGGA 
AAAGCAGAAT GTGCTTGCAA AGTGCTTGTG GAAGATACCT CATCCACAAA GGCTGCGAAG 
CCAGCTGAGA AAAAGACCAA GAAGCCTAAG ACCACACTTC CCCCTGTGCT GTCAACAGAA 
AGCAGTGAAG CAACAGTGAA GAAGAAACCA GCTCCAAAGA CTCCTCCAAA AGCAGCTACT 
CCCCCTCAGA TCACTCAGTT CCCAGAAGAC AGAAAAGTCC GTGCTGGTGA ATCAGTGGAA 
TTGTTTGCCA AAGTGGTAGG AACAGCACCC ATAACTTGCA CCTGGATGAA ATTCCGTAAG 
CAGATTCAAG AAAATGAATA TATCAAAATT GAGAATGCTG AAAACAGCAG CAAGCTAACA 
ATATCATCGA CAAAGCAGGA ACACTGCGGA TGTTACACGC TAGTTGTAGA AAACAAACTT 
GGCAGCAGAC AAGCACAAGT CAACCTTACA GTTGTTGATA AACCAGACCC ACCTGCTGGA 
ACGCCTTGTG CATCAGACAT ACGGAGTTCT TCCCTCACTC TTTCATGGTA CGGCTCCTCT 
TATGATGGTG GAAGTGCGGT GCAGTCCTAC ACTGTGGAGA TCTGGAACTC AGTGGATAAC 
AAATGGACAG ACCTCACTAC CTGCCGCAGC ACGTCTTTCA ACGTGCAGGA CCTGCAGGCT 
GACCGGGAGT ACAAATTCCG TGTACGAGCT GCTAACGTGT ACGGCATCAG TGAGCCCAGT 
CAAGAATCTG AGGTGGTAAA AGTTGGAGAG AAACAAGAAG AAGAACTTAA AGAGGAGGAA 
GCAGAGCTGT CTGACGATGA AGGCAAAGAA ACAGAGGTCA ACTATCGGAC AGTTACTATC 
AACACCGAAC AGAAAGTTTC AGATGTCTAT AATATTGAAG AAAGACTGGG ATCGGGGAAA 
TTTGGACAAG TCTTTAGGCT TGTTGAAAAG AAGACTGGAA AAGTGTGGGC AGGAAAATTT 
TTCAAAGCAT ATTCTGCAAA GGAAAAAGAG AACATAAGGG ATGAAATCAG CATCATGAAC 
TGTCTGCATC ACCCAAAGCT CGTGCAATGT GTAGATGCCT TTGAAGAAAA AGCCAACATT 
GTTATGGTCT TGGAAATGGT TTCTGGAGGA GAACTCTTTG AACGTATCAT TGATGAAGAC 
TTTGAACTGA CAGAGAGAGA ATGCATTAAA TATATGAGGC AGATTTCAGA AGGAGTTGAG 
TACATCCATA AGCAGGGTAT TGTCCATTTG GATTTGAAGC CAGAAAATAT TATGTGTGTC 
AACAAGACTG GTACAAGTAT CAAACTCATT GACTTCGGAC TTGCAAGAAG ATTAGAAAGT 
GCAGGTTCTC TCAAAGTTCT CTTTGGGACA CCTGAGTTTG TGGCTCCAGA AGTTATAAAC 
TATGAACCTA TCGGATATGA AACAGATATG TGGAGTATAG GAGTTATCTG CTACATCCTG 
GTCAGCGGGC TTTCTCCCTT CATGGGAGAC AACGACAACG AGACCCTGGC CAACGTCACC 
TCTGCCACGT GGGACTTCGA TGATGAGGCT TTTGATGAAA TCTCTGACGA TGCCAAGGAC 
TTTATCAGCA ATCTCCTGAA GAAAGACATG AAGAGTCGCT TAAATTGTAC TCAATGTCTT 
CAGCACCCGT GGCTGCAAAA AGACACCAAA AACATGGAAG CCAAAAAACT TTCCAAAGAT 
AGGATGAAGA AATATATGGC CAGAAGAAAA TGGCAGAAAA CAGGCCATGC TGTCCGAGCA 
ATAGGAAGAC TGTCATCCAT GGCAATGATT TCTGGTATGA GTGGGAGGAA GGCCTCTGGG 
AGCTCGCCTA CCAGCCCGAT AAATGCAGAC AAAGTAGAGA ACGAAGATGC CTTCCTTGAA 
GAAGTGGCTG AGGAAAAGCC CCATGTAAAG CCATACTTTA CTAAAACAAT CCTTGACATG 
GAGGTTGTGG AAGGAAGTGC TGCTAGGTTT GACTGCAAAA TCGAAGGCTA TCCTGACCCT 
GAGGTAATGT GGTACAAAGA TGATCAGCCT GTCAAGGAGT CCCGTCACTT CCAGATAGAT 
TATGATGAGG AAGGGAACTG TTCTTTGACT ATTTCTGAAG TATGTGGGGA CGATGATGCC 
AAATACACCT GCAAAGCTGT TAACAGCCTT GGGGAAGCCA CATGTACCGC AGAACTCCTT 
GTGGAAACAA TGGGAAAAGA GGGAGAAGGA GAAGGAGAAG GAGAAGAAGA TGAAGAGGAG 
GAGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates.
Cell biochemistry and function34(7)469-474(2016 Oct)
Alcala DB,Haldeman BD,Brizendine RK,Krenc AK,Baker JE,Rock RS,Cremo CR

Kinetics of myosin light chain kinase activation of smooth muscle myosin in an in vitro model system.
Biochemistry52(47)8489-500(2013 Nov)
Hong F,Facemyer KC,Carter MS,Jackson del R,Haldeman BD,Ruana N,Sutherland C,Walsh MP,Cremo CR,Baker JE

Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells.
Journal of pharmacological sciences116(1)116-27(2011)
Thatcher SE,Fultz ME,Tanaka H,Hagiwara H,Zhang HL,Zhang Y,Hayakawa K,Yoshiyama S,Nakamura A,Wang HH,Katayama T,Watanabe M,Lin Y,Wright GL,Kohama K

Characterization of tightly associated smooth muscle myosin-myosin light-chain kinase-calmodulin complexes.
Journal of molecular biology390(5)879-92(2009 Jul)
Hong F,Haldeman BD,John OA,Brewer PD,Wu YY,Ni S,Wilson DP,Walsh MP,Baker JE,Cremo CR

Two distinct modes of myosin assembly and dynamics during epithelial wound closure.
The Journal of cell biology176(1)27-33(2007 Jan)
Tamada M,Perez TD,Nelson WJ,Sheetz MP

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MYLK cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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