CACNA1B cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1B
Entrez Gene ID	374169
Full Name	calcium voltage-gated channel subunit alpha1 B
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 B Names voltage-dependent N-type calcium channel subunit alpha-1B CHCACHA1B N-type voltage-gated calcium channel alpha1B subunit ChCaChA1B calcium channel, voltage-dependent, L type, alpha 1B subunit calcium channel, voltage-dependent, N type, alpha 1B subunit voltage-gated calcium channel subunit alpha Cav2.2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015279263.1	XP_015134749.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_015279266.1	XP_015134752.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
XM_015279267.1	XP_015134753.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
XM_015279270.1	XP_015134756.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
XM_015279272.1	XP_015134758.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
XM_015279273.1	XP_015134759.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
XM_015279274.1	XP_015134760.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
XM_015279261.2	XP_015134747.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_015279265.2	XP_015134751.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
XM_015279266.2	XP_015134752.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
XM_015279267.2	XP_015134753.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
XM_015279261.1	XP_015134747.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_015279279.1	XP_015134765.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X19
XM_015279264.2	XP_015134750.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_015279277.2	XP_015134763.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X19
XM_015279278.2	XP_015134764.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X20
XM_015279279.2	XP_015134765.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X21
XM_015279262.1	XP_015134748.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_015279264.1	XP_015134750.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_015279265.1	XP_015134751.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
XM_015279269.1	XP_015134755.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
XM_015279281.1	XP_015134767.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X21
XM_025156020.1	XP_025011788.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
XM_015279276.2	XP_015134762.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X18
NM_204293.1	NP_989624.1	voltage-dependent N-type calcium channel subunit alpha-1B
XM_015279275.1	XP_015134761.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
XM_015279277.1	XP_015134763.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
XM_015279263.2	XP_015134749.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_015279271.2	XP_015134757.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
XM_015279272.2	XP_015134758.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
XM_015279275.2	XP_015134761.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
XM_015279268.1	XP_015134754.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
XM_015279271.1	XP_015134757.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
XM_015279276.1	XP_015134762.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
XM_015279278.1	XP_015134764.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X18
XM_015279280.1	XP_015134766.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X20
XM_015279262.2	XP_015134748.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_025156019.1	XP_025011787.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
XM_015279268.2	XP_015134754.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
XM_015279269.2	XP_015134755.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
XM_015279270.2	XP_015134756.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
XM_015279273.2	XP_015134759.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
XM_015279274.2	XP_015134760.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CACNA1B	XP_520396.4
Canis lupus familiaris (dog)	CACNA1B	XP_005625483.1
Rattus norvegicus (Norway rat)	Cacna1b	NP_671482.1
Danio rerio (zebrafish)	cacna1ba	NP_001108020.1
Caenorhabditis elegans (roundworm)	unc-2	NP_001123176.1
Gallus gallus (chicken)	CACNA1B	NP_989624.1
Drosophila melanogaster (fruit fly)	cac	NP_001245636.1
Homo sapiens (human)	CACNA1B	NP_000709.1
Bos taurus (cattle)	CACNA1B	NP_777057.1
Mus musculus (house mouse)	Cacna1b	NP_001035993.1
Danio rerio (zebrafish)	cacna1bb	XP_002665969.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002578	XP_312358.4

Gene Ontology
Bibliography

Related articles in PubMed

Long C terminal splice variant CaV2.2 identified in presynaptic membrane by mass spectrometric analysis.
Gardezi SR, Taylor P, Stanley EF
Channels (Austin, Tex.)4(1)58-62(2010 Jan-Feb)

Munc18: a presynaptic transmitter release site N type (CaV2.2) calcium channel interacting protein.
Chan AW, Khanna R, Li Q, Stanley EF
Channels (Austin, Tex.)1(1)11-20(2007 Jan-Feb)

RGS12 interacts with the SNARE-binding region of the Cav2.2 calcium channel.
Richman RW, Strock J, Hains MD, Cabanilla NJ, Lau KK, Siderovski DP, Divers?-Pierluissi M
The Journal of biological chemistry280(2)1521-8(2005 Jan)

Cloning and functional expression of novel N-type Ca(2+) channel variants.
L? Q, Dunlap K
The Journal of biological chemistry274(49)34566-75(1999 Dec)

Predominance of the alpha1D subunit in L-type voltage-gated Ca2+ channels of hair cells in the chicken's cochlea.
Kollmar R, Montgomery LG, Fak J, Henry LJ, Hudspeth AJ
Proceedings of the National Academy of Sciences of the United States of America94(26)14883-8(1997 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identify of the long-splice Ca(V)2.2 in synaptosomes prepared from chick embryo brain.
Title: Long C terminal splice variant CaV2.2 identified in presynaptic membrane by mass spectrometric analysis.

Munc18: a presynaptic transmitter release site N type (CaV2.2) calcium channel interacting protein.
Title: Munc18: a presynaptic transmitter release site N type (CaV2.2) calcium channel interacting protein.

The following CACNA1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa145070	XM_015279261.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145071	XM_015279262.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145072	XM_015279263.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145073	XM_015279264.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145074	XM_015279265.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145075	XM_015279266.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145076	XM_015279267.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145077	XM_025156019.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145078	XM_015279268.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145079	XM_015279269.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145080	XM_015279270.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145081	XM_015279271.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa145082	XM_015279272.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa145083	XM_015279273.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa145084	XM_015279274.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa145085	XM_025156020.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa145086	XM_015279275.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa145087	XM_015279276.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa145088	XM_015279277.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa145089	XM_015279278.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa145090	XM_015279279.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa43287	XM_015279261.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43288	XM_015279262.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43289	XM_015279263.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43290	XM_015279264.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43291	XM_015279265.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43292	XM_015279266.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43293	XM_015279267.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43294	XM_015279268.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43295	XM_015279269.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43296	XM_015279270.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43297	XM_015279271.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa43298	XM_015279272.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43299	XM_015279273.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43300	XM_015279274.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43301	XM_015279275.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43302	XM_015279276.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43303	XM_015279277.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43304	XM_015279278.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43305	XM_015279279.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43306	XM_015279280.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43307	XM_015279281.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OGa26844	NM_204293.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1B ( XM_015279261.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134747.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa145070
Clone ID Related Accession (Same CDS sequence)	XM_015279261.2
Accession Version	XM_015279261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ATATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGACTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGCGGGCGC ATCAGTTACA CAGACATGTA TGAGATGCTG 
AGACACATGT CCCCACCTCT AGGCCTTGGA AAGAAATGCC CTGCTCGCGT TGCCTATAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGGGCA GTGGTTCGGT TAACGGGAGC 
CCCTTGCTGT CGACATCTGG TGCTAGTACC CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG 
ACCCCCCTGA CCCCACGCCC CAGCATCACT TACAAGACCG CCAACTCCTC GCCCGTACAC 
TTCAGCACGT CGCCGGGCGG CCTCCCGCCC TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG 
TCTGAGCACA ATGCACTGCT GCGTGGGGAC CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC 
ATCGGCTCCG ACCCTTACCT GGGCCACCGC GACGCCGCCG ACAGCCCCAT AGGCGCAGCG 
CCCGAGGACA CGCTCACCTT CGAGGAAGCC GTGGCCACCA ACTCGGGGCG TTCCTCCAGG 
ACTTCTTACG TCTCCTCCTT GACCTCCCAG TCTCACCAAG CACGCCGGGT GCCCAATGGC 
TACCATTACA CGCTGGGGCT CAACACGGGG CCCGGCACTG GCACCAGGGG ACGGAGCTAC 
TACCACGAAG CCGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134747.2
CDS	422..7534
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279261.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279261.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ATATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGACTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGCGGGCGC ATCAGTTACA CAGACATGTA TGAGATGCTG 
AGACACATGT CCCCACCTCT AGGCCTTGGA AAGAAATGCC CTGCTCGCGT TGCCTATAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGGGCA GTGGTTCGGT TAACGGGAGC 
CCCTTGCTGT CGACATCTGG TGCTAGTACC CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG 
ACCCCCCTGA CCCCACGCCC CAGCATCACT TACAAGACCG CCAACTCCTC GCCCGTACAC 
TTCAGCACGT CGCCGGGCGG CCTCCCGCCC TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG 
TCTGAGCACA ATGCACTGCT GCGTGGGGAC CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC 
ATCGGCTCCG ACCCTTACCT GGGCCACCGC GACGCCGCCG ACAGCCCCAT AGGCGCAGCG 
CCCGAGGACA CGCTCACCTT CGAGGAAGCC GTGGCCACCA ACTCGGGGCG TTCCTCCAGG 
ACTTCTTACG TCTCCTCCTT GACCTCCCAG TCTCACCAAG CACGCCGGGT GCCCAATGGC 
TACCATTACA CGCTGGGGCT CAACACGGGG CCCGGCACTG GCACCAGGGG ACGGAGCTAC 
TACCACGAAG CCGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279262.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134748.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.

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CloneID	OGa145071
Clone ID Related Accession (Same CDS sequence)	XM_015279262.2
Accession Version	XM_015279262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ATATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGACTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGTTGCCGG ATTCATTATA AGGATATGTA CAATTTGTTA 
CGTGTAATTG CTCCACCCCT GGGCTTAGGA AAGAAGTGCC CTCATAGGGT GGCATACAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGGGCA GTGGTTCGGT TAACGGGAGC 
CCCTTGCTGT CGACATCTGG TGCTAGTACC CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG 
ACCCCCCTGA CCCCACGCCC CAGCATCACT TACAAGACCG CCAACTCCTC GCCCGTACAC 
TTCAGCACGT CGCCGGGCGG CCTCCCGCCC TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG 
TCTGAGCACA ATGCACTGCT GCGTGGGGAC CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC 
ATCGGCTCCG ACCCTTACCT GGGCCACCGC GACGCCGCCG ACAGCCCCAT AGGCGCAGCG 
CCCGAGGACA CGCTCACCTT CGAGGAAGCC GTGGCCACCA ACTCGGGGCG TTCCTCCAGG 
ACTTCTTACG TCTCCTCCTT GACCTCCCAG TCTCACCAAG CACGCCGGGT GCCCAATGGC 
TACCATTACA CGCTGGGGCT CAACACGGGG CCCGGCACTG GCACCAGGGG ACGGAGCTAC 
TACCACGAAG CCGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134748.2
CDS	422..7534
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279262.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279262.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ATATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGACTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGTTGCCGG ATTCATTATA AGGATATGTA CAATTTGTTA 
CGTGTAATTG CTCCACCCCT GGGCTTAGGA AAGAAGTGCC CTCATAGGGT GGCATACAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGGGCA GTGGTTCGGT TAACGGGAGC 
CCCTTGCTGT CGACATCTGG TGCTAGTACC CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG 
ACCCCCCTGA CCCCACGCCC CAGCATCACT TACAAGACCG CCAACTCCTC GCCCGTACAC 
TTCAGCACGT CGCCGGGCGG CCTCCCGCCC TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG 
TCTGAGCACA ATGCACTGCT GCGTGGGGAC CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC 
ATCGGCTCCG ACCCTTACCT GGGCCACCGC GACGCCGCCG ACAGCCCCAT AGGCGCAGCG 
CCCGAGGACA CGCTCACCTT CGAGGAAGCC GTGGCCACCA ACTCGGGGCG TTCCTCCAGG 
ACTTCTTACG TCTCCTCCTT GACCTCCCAG TCTCACCAAG CACGCCGGGT GCCCAATGGC 
TACCATTACA CGCTGGGGCT CAACACGGGG CCCGGCACTG GCACCAGGGG ACGGAGCTAC 
TACCACGAAG CCGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279263.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134749.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.

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CloneID	OGa145072
Clone ID Related Accession (Same CDS sequence)	XM_015279263.2
Accession Version	XM_015279263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ATATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACAACT TCATCAACCT CAGCTTCCTG CGGCTCTTCA GGGCAGCAAG ACTTATCAAG 
CTCCTTCGCC AGGGTTACAC TATCCGCATC TTGCTCTGGA CGTTTGTGCA GTCATTTAAG 
GCCTTGCCTT ATGTGTGTCT TCTCATTGCA ATGCTCTTCT TCATCTATGC CATTATTGGC 
ATGCAGGTAT TTGGAAACAT TGCACTAAAT GACGAAACCT CCATCAATCG GCACAATAAT 
TTCCGTACCT TCCTTCAAGC CTTGATGCTT CTGTTCAGGA GTGCCACGGG GGAGGCCTGG 
CACGAGATCA TGCTGTCATG CCTGAGCAAC AGGGCCTGCG ACCCACTCTC CGGCCTCACC 
AAGAACGAGT GCGGCAGCGA CTTCGCCTAC TTCTACTTCG TGTCATTCAT CTTCCTCTGC 
TCCTTCCTGA TGTTGAACCT ATTTGTGGCT GTAATCATGG ACAACTTTGA ATACTTGACA 
AGAGACTCCT CCATCCTTGG GCCCCATCAT TTGGATGAGT TTGTCCGTGT CTGGGCTGAA 
TACGACCCAG CTGCCTGCGG GCGCATCAGT TACACAGACA TGTATGAGAT GCTGAGACAC 
ATGTCCCCAC CTCTAGGCCT TGGAAAGAAA TGCCCTGCTC GCGTTGCCTA TAAGCGCCTG 
GTGCGGATGA ACATGCCCAT CTCACCCGAG GATCTGACAG TCCACTTCAC CTCCACGCTG 
ATGGCCCTGA TCCGCACTGC TCTGGAAATC AAGCTCGCCT CAGGGGGTGT TAAGCAGCAC 
CAGTGTGATG CTGAGCTGAG AAAGGAGATA TCTCTAGTTT GGCCCAACCT GTCCCAGAAG 
ACACTGGATT TGCTGGTGCC TCCTCACAAA CCTGATGAGA TGACTGTGGG GAAGGTCTAC 
GCAGCGCTCA TGATATTTGA CTTCTACAAA CAGAACAAGA ACAGCAGGGA ACAAGTCCAC 
CAGCCTCCTG GGGGCCTGTG CCAGCCCGGA CCAGTGTCAC TCTTCCACCC CTTGAAGGCC 
ACTTTGGAGC AAACCCAGCC CTCGGCATTC AGCAGTGCGA AGGCATTCCT GCGCCAGAAA 
AGCTCGGCCT CACTCAACAA TGGAGGCACA TTGCCAGCCC CAGAGGGTGG GATCAAAGAG 
TCCAGTTCGT GGGGGACCCA ACGGACCCAG GACGTGTTTT ATGAAACCAG GACACCAGCT 
TTTGAACGAG GCCACTCAGA GGAAATCCCC ATTGAACGGG TGGTGGAAAT GAGGGAAATC 
AGCCCCACAC TTGCCAATGG AGAGCACCAA CCAGGGCTGG AGAGCCAGGG TCGGGCGGCT 
TCCATGCCGC GCCTGGCTGC TGAAACGCAG CCCATCCCGG ACACAAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGACCCC GCAGCGTCCT CACCCCATGC ACCTCTATGA GTACTCCCTG 
GAGCGCGTGC CCACGGACCA GGTGCATCAC CACCACCACC ACCGCTGCCA CCGGCGGAAA 
GAGAAGAAGC AGAAGTCCTT GGACAGGACC ACACATCAGC TGGCCGATGG GGAAGCTGTG 
GCCCAGTCTG GTGAATCTTC TTCCAAGGAC AAGAAGCAAG AGCGTGGACG GTCCCAAGAG 
AGGAAGCAGC ACTCTTCCTC CTCCTCAGAA AAGCAGCGCT TCTACTCCTG TGATCGCTAT 
GGCAGCCGGG ACCGATCCCA GCCCAAGTCT GCTGATCAGA GCAGGCCTAC TTCACCCAAT 
GGTGGGCCTG AGCAAGGTCC CCACAGACAG GGCAGTGGTT CGGTTAACGG GAGCCCCTTG 
CTGTCGACAT CTGGTGCTAG TACCCCCTGC CGGGGTCGGA GGCAGCTCCC GCAGACCCCC 
CTGACCCCAC GCCCCAGCAT CACTTACAAG ACCGCCAACT CCTCGCCCGT ACACTTCAGC 
ACGTCGCCGG GCGGCCTCCC GCCCTTCTCC CCGGGCCGCC TGAGCCGCGG GCTGTCTGAG 
CACAATGCAC TGCTGCGTGG GGACCAGCAG CCGCCCCCGG CCGCGGTTGC CCGCATCGGC 
TCCGACCCTT ACCTGGGCCA CCGCGACGCC GCCGACAGCC CCATAGGCGC AGCGCCCGAG 
GACACGCTCA CCTTCGAGGA AGCCGTGGCC ACCAACTCGG GGCGTTCCTC CAGGACTTCT 
TACGTCTCCT CCTTGACCTC CCAGTCTCAC CAAGCACGCC GGGTGCCCAA TGGCTACCAT 
TACACGCTGG GGCTCAACAC GGGGCCCGGC ACTGGCACCA GGGGACGGAG CTACTACCAC 
GAAGCCGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134749.2
CDS	422..7528
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279263.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279263.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ATATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACAACT TCATCAACCT CAGCTTCCTG CGGCTCTTCA GGGCAGCAAG ACTTATCAAG 
CTCCTTCGCC AGGGTTACAC TATCCGCATC TTGCTCTGGA CGTTTGTGCA GTCATTTAAG 
GCCTTGCCTT ATGTGTGTCT TCTCATTGCA ATGCTCTTCT TCATCTATGC CATTATTGGC 
ATGCAGGTAT TTGGAAACAT TGCACTAAAT GACGAAACCT CCATCAATCG GCACAATAAT 
TTCCGTACCT TCCTTCAAGC CTTGATGCTT CTGTTCAGGA GTGCCACGGG GGAGGCCTGG 
CACGAGATCA TGCTGTCATG CCTGAGCAAC AGGGCCTGCG ACCCACTCTC CGGCCTCACC 
AAGAACGAGT GCGGCAGCGA CTTCGCCTAC TTCTACTTCG TGTCATTCAT CTTCCTCTGC 
TCCTTCCTGA TGTTGAACCT ATTTGTGGCT GTAATCATGG ACAACTTTGA ATACTTGACA 
AGAGACTCCT CCATCCTTGG GCCCCATCAT TTGGATGAGT TTGTCCGTGT CTGGGCTGAA 
TACGACCCAG CTGCCTGCGG GCGCATCAGT TACACAGACA TGTATGAGAT GCTGAGACAC 
ATGTCCCCAC CTCTAGGCCT TGGAAAGAAA TGCCCTGCTC GCGTTGCCTA TAAGCGCCTG 
GTGCGGATGA ACATGCCCAT CTCACCCGAG GATCTGACAG TCCACTTCAC CTCCACGCTG 
ATGGCCCTGA TCCGCACTGC TCTGGAAATC AAGCTCGCCT CAGGGGGTGT TAAGCAGCAC 
CAGTGTGATG CTGAGCTGAG AAAGGAGATA TCTCTAGTTT GGCCCAACCT GTCCCAGAAG 
ACACTGGATT TGCTGGTGCC TCCTCACAAA CCTGATGAGA TGACTGTGGG GAAGGTCTAC 
GCAGCGCTCA TGATATTTGA CTTCTACAAA CAGAACAAGA ACAGCAGGGA ACAAGTCCAC 
CAGCCTCCTG GGGGCCTGTG CCAGCCCGGA CCAGTGTCAC TCTTCCACCC CTTGAAGGCC 
ACTTTGGAGC AAACCCAGCC CTCGGCATTC AGCAGTGCGA AGGCATTCCT GCGCCAGAAA 
AGCTCGGCCT CACTCAACAA TGGAGGCACA TTGCCAGCCC CAGAGGGTGG GATCAAAGAG 
TCCAGTTCGT GGGGGACCCA ACGGACCCAG GACGTGTTTT ATGAAACCAG GACACCAGCT 
TTTGAACGAG GCCACTCAGA GGAAATCCCC ATTGAACGGG TGGTGGAAAT GAGGGAAATC 
AGCCCCACAC TTGCCAATGG AGAGCACCAA CCAGGGCTGG AGAGCCAGGG TCGGGCGGCT 
TCCATGCCGC GCCTGGCTGC TGAAACGCAG CCCATCCCGG ACACAAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGACCCC GCAGCGTCCT CACCCCATGC ACCTCTATGA GTACTCCCTG 
GAGCGCGTGC CCACGGACCA GGTGCATCAC CACCACCACC ACCGCTGCCA CCGGCGGAAA 
GAGAAGAAGC AGAAGTCCTT GGACAGGACC ACACATCAGC TGGCCGATGG GGAAGCTGTG 
GCCCAGTCTG GTGAATCTTC TTCCAAGGAC AAGAAGCAAG AGCGTGGACG GTCCCAAGAG 
AGGAAGCAGC ACTCTTCCTC CTCCTCAGAA AAGCAGCGCT TCTACTCCTG TGATCGCTAT 
GGCAGCCGGG ACCGATCCCA GCCCAAGTCT GCTGATCAGA GCAGGCCTAC TTCACCCAAT 
GGTGGGCCTG AGCAAGGTCC CCACAGACAG GGCAGTGGTT CGGTTAACGG GAGCCCCTTG 
CTGTCGACAT CTGGTGCTAG TACCCCCTGC CGGGGTCGGA GGCAGCTCCC GCAGACCCCC 
CTGACCCCAC GCCCCAGCAT CACTTACAAG ACCGCCAACT CCTCGCCCGT ACACTTCAGC 
ACGTCGCCGG GCGGCCTCCC GCCCTTCTCC CCGGGCCGCC TGAGCCGCGG GCTGTCTGAG 
CACAATGCAC TGCTGCGTGG GGACCAGCAG CCGCCCCCGG CCGCGGTTGC CCGCATCGGC 
TCCGACCCTT ACCTGGGCCA CCGCGACGCC GCCGACAGCC CCATAGGCGC AGCGCCCGAG 
GACACGCTCA CCTTCGAGGA AGCCGTGGCC ACCAACTCGG GGCGTTCCTC CAGGACTTCT 
TACGTCTCCT CCTTGACCTC CCAGTCTCAC CAAGCACGCC GGGTGCCCAA TGGCTACCAT 
TACACGCTGG GGCTCAACAC GGGGCCCGGC ACTGGCACCA GGGGACGGAG CTACTACCAC 
GAAGCCGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279264.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134750.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.

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CloneID	OGa145073
Clone ID Related Accession (Same CDS sequence)	XM_015279264.2
Accession Version	XM_015279264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279264.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAT ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACACGGAC 
AACTTCATCA ACCTCAGCTT CCTGCGGCTC TTCAGGGCAG CAAGACTTAT CAAGCTCCTT 
CGCCAGGGTT ACACTATCCG CATCTTGCTC TGGACGTTTG TGCAGTCATT TAAGGCCTTG 
CCTTATGTGT GTCTTCTCAT TGCAATGCTC TTCTTCATCT ATGCCATTAT TGGCATGCAG 
GTATTTGGAA ACATTGCACT AAATGACGAA ACCTCCATCA ATCGGCACAA TAATTTCCGT 
ACCTTCCTTC AAGCCTTGAT GCTTCTGTTC AGGAGTGCCA CGGGGGAGGC CTGGCACGAG 
ATCATGCTGT CATGCCTGAG CAACAGGGCC TGCGACCCAC TCTCCGGCCT CACCAAGAAC 
GAGTGCGGCA GCGACTTCGC CTACTTCTAC TTCGTGTCAT TCATCTTCCT CTGCTCCTTC 
CTGATGTTGA ACCTATTTGT GGCTGTAATC ATGGACAACT TTGAATACTT GACAAGAGAC 
TCCTCCATCC TTGGGCCCCA TCATTTGGAT GAGTTTGTCC GTGTCTGGGC TGAATACGAC 
CCAGCTGCCT GCGGGCGCAT CAGTTACACA GACATGTATG AGATGCTGAG ACACATGTCC 
CCACCTCTAG GCCTTGGAAA GAAATGCCCT GCTCGCGTTG CCTATAAGCG CCTGGTGCGG 
ATGAACATGC CCATCTCACC CGAGGATCTG ACAGTCCACT TCACCTCCAC GCTGATGGCC 
CTGATCCGCA CTGCTCTGGA AATCAAGCTC GCCTCAGGGG GTGTTAAGCA GCACCAGTGT 
GATGCTGAGC TGAGAAAGGA GATATCTCTA GTTTGGCCCA ACCTGTCCCA GAAGACACTG 
GATTTGCTGG TGCCTCCTCA CAAACCTGAT GAGATGACTG TGGGGAAGGT CTACGCAGCG 
CTCATGATAT TTGACTTCTA CAAACAGAAC AAGAACAGCA GGGAACAAGT CCACCAGCCT 
CCTGGGGGCC TGTGCCAGCC CGGACCAGTG TCACTCTTCC ACCCCTTGAA GGCCACTTTG 
GAGCAAACCC AGCCCTCGGC ATTCAGCAGT GCGAAGGCAT TCCTGCGCCA GAAAAGCTCG 
GCCTCACTCA ACAATGGAGG CACATTGCCA GCCCCAGAGG GTGGGATCAA AGAGTCCAGT 
TCGTGGGGGA CCCAACGGAC CCAGGACGTG TTTTATGAAA CCAGGACACC AGCTTTTGAA 
CGAGGCCACT CAGAGGAAAT CCCCATTGAA CGGGTGGTGG AAATGAGGGA AATCAGCCCC 
ACACTTGCCA ATGGAGAGCA CCAACCAGGG CTGGAGAGCC AGGGTCGGGC GGCTTCCATG 
CCGCGCCTGG CTGCTGAAAC GCAGCCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGGGCAGT GGTTCGGTTA ACGGGAGCCC CTTGCTGTCG 
ACATCTGGTG CTAGTACCCC CTGCCGGGGT CGGAGGCAGC TCCCGCAGAC CCCCCTGACC 
CCACGCCCCA GCATCACTTA CAAGACCGCC AACTCCTCGC CCGTACACTT CAGCACGTCG 
CCGGGCGGCC TCCCGCCCTT CTCCCCGGGC CGCCTGAGCC GCGGGCTGTC TGAGCACAAT 
GCACTGCTGC GTGGGGACCA GCAGCCGCCC CCGGCCGCGG TTGCCCGCAT CGGCTCCGAC 
CCTTACCTGG GCCACCGCGA CGCCGCCGAC AGCCCCATAG GCGCAGCGCC CGAGGACACG 
CTCACCTTCG AGGAAGCCGT GGCCACCAAC TCGGGGCGTT CCTCCAGGAC TTCTTACGTC 
TCCTCCTTGA CCTCCCAGTC TCACCAAGCA CGCCGGGTGC CCAATGGCTA CCATTACACG 
CTGGGGCTCA ACACGGGGCC CGGCACTGGC ACCAGGGGAC GGAGCTACTA CCACGAAGCC 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134750.2
CDS	421..7521
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279264.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279264.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAT ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACACGGAC 
AACTTCATCA ACCTCAGCTT CCTGCGGCTC TTCAGGGCAG CAAGACTTAT CAAGCTCCTT 
CGCCAGGGTT ACACTATCCG CATCTTGCTC TGGACGTTTG TGCAGTCATT TAAGGCCTTG 
CCTTATGTGT GTCTTCTCAT TGCAATGCTC TTCTTCATCT ATGCCATTAT TGGCATGCAG 
GTATTTGGAA ACATTGCACT AAATGACGAA ACCTCCATCA ATCGGCACAA TAATTTCCGT 
ACCTTCCTTC AAGCCTTGAT GCTTCTGTTC AGGAGTGCCA CGGGGGAGGC CTGGCACGAG 
ATCATGCTGT CATGCCTGAG CAACAGGGCC TGCGACCCAC TCTCCGGCCT CACCAAGAAC 
GAGTGCGGCA GCGACTTCGC CTACTTCTAC TTCGTGTCAT TCATCTTCCT CTGCTCCTTC 
CTGATGTTGA ACCTATTTGT GGCTGTAATC ATGGACAACT TTGAATACTT GACAAGAGAC 
TCCTCCATCC TTGGGCCCCA TCATTTGGAT GAGTTTGTCC GTGTCTGGGC TGAATACGAC 
CCAGCTGCCT GCGGGCGCAT CAGTTACACA GACATGTATG AGATGCTGAG ACACATGTCC 
CCACCTCTAG GCCTTGGAAA GAAATGCCCT GCTCGCGTTG CCTATAAGCG CCTGGTGCGG 
ATGAACATGC CCATCTCACC CGAGGATCTG ACAGTCCACT TCACCTCCAC GCTGATGGCC 
CTGATCCGCA CTGCTCTGGA AATCAAGCTC GCCTCAGGGG GTGTTAAGCA GCACCAGTGT 
GATGCTGAGC TGAGAAAGGA GATATCTCTA GTTTGGCCCA ACCTGTCCCA GAAGACACTG 
GATTTGCTGG TGCCTCCTCA CAAACCTGAT GAGATGACTG TGGGGAAGGT CTACGCAGCG 
CTCATGATAT TTGACTTCTA CAAACAGAAC AAGAACAGCA GGGAACAAGT CCACCAGCCT 
CCTGGGGGCC TGTGCCAGCC CGGACCAGTG TCACTCTTCC ACCCCTTGAA GGCCACTTTG 
GAGCAAACCC AGCCCTCGGC ATTCAGCAGT GCGAAGGCAT TCCTGCGCCA GAAAAGCTCG 
GCCTCACTCA ACAATGGAGG CACATTGCCA GCCCCAGAGG GTGGGATCAA AGAGTCCAGT 
TCGTGGGGGA CCCAACGGAC CCAGGACGTG TTTTATGAAA CCAGGACACC AGCTTTTGAA 
CGAGGCCACT CAGAGGAAAT CCCCATTGAA CGGGTGGTGG AAATGAGGGA AATCAGCCCC 
ACACTTGCCA ATGGAGAGCA CCAACCAGGG CTGGAGAGCC AGGGTCGGGC GGCTTCCATG 
CCGCGCCTGG CTGCTGAAAC GCAGCCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGGGCAGT GGTTCGGTTA ACGGGAGCCC CTTGCTGTCG 
ACATCTGGTG CTAGTACCCC CTGCCGGGGT CGGAGGCAGC TCCCGCAGAC CCCCCTGACC 
CCACGCCCCA GCATCACTTA CAAGACCGCC AACTCCTCGC CCGTACACTT CAGCACGTCG 
CCGGGCGGCC TCCCGCCCTT CTCCCCGGGC CGCCTGAGCC GCGGGCTGTC TGAGCACAAT 
GCACTGCTGC GTGGGGACCA GCAGCCGCCC CCGGCCGCGG TTGCCCGCAT CGGCTCCGAC 
CCTTACCTGG GCCACCGCGA CGCCGCCGAC AGCCCCATAG GCGCAGCGCC CGAGGACACG 
CTCACCTTCG AGGAAGCCGT GGCCACCAAC TCGGGGCGTT CCTCCAGGAC TTCTTACGTC 
TCCTCCTTGA CCTCCCAGTC TCACCAAGCA CGCCGGGTGC CCAATGGCTA CCATTACACG 
CTGGGGCTCA ACACGGGGCC CGGCACTGGC ACCAGGGGAC GGAGCTACTA CCACGAAGCC 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279265.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134751.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.

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CloneID	OGa145074
Clone ID Related Accession (Same CDS sequence)	XM_015279265.2
Accession Version	XM_015279265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAT ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACAACTTC 
ATCAACCTCA GCTTCCTGCG GCTCTTCAGG GCAGCAAGAC TTATCAAGCT CCTTCGCCAG 
GGTTACACTA TCCGCATCTT GCTCTGGACG TTTGTGCAGT CATTTAAGGC CTTGCCTTAT 
GTGTGTCTTC TCATTGCAAT GCTCTTCTTC ATCTATGCCA TTATTGGCAT GCAGGTATTT 
GGAAACATTG CACTAAATGA CGAAACCTCC ATCAATCGGC ACAATAATTT CCGTACCTTC 
CTTCAAGCCT TGATGCTTCT GTTCAGGAGT GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCATGCC TGAGCAACAG GGCCTGCGAC CCACTCTCCG GCCTCACCAA GAACGAGTGC 
GGCAGCGACT TCGCCTACTT CTACTTCGTG TCATTCATCT TCCTCTGCTC CTTCCTGATG 
TTGAACCTAT TTGTGGCTGT AATCATGGAC AACTTTGAAT ACTTGACAAG AGACTCCTCC 
ATCCTTGGGC CCCATCATTT GGATGAGTTT GTCCGTGTCT GGGCTGAATA CGACCCAGCT 
GCCTGCGGGC GCATCAGTTA CACAGACATG TATGAGATGC TGAGACACAT GTCCCCACCT 
CTAGGCCTTG GAAAGAAATG CCCTGCTCGC GTTGCCTATA AGCGCCTGGT GCGGATGAAC 
ATGCCCATCT CACCCGAGGA TCTGACAGTC CACTTCACCT CCACGCTGAT GGCCCTGATC 
CGCACTGCTC TGGAAATCAA GCTCGCCTCA GGGGGTGTTA AGCAGCACCA GTGTGATGCT 
GAGCTGAGAA AGGAGATATC TCTAGTTTGG CCCAACCTGT CCCAGAAGAC ACTGGATTTG 
CTGGTGCCTC CTCACAAACC TGATGAGATG ACTGTGGGGA AGGTCTACGC AGCGCTCATG 
ATATTTGACT TCTACAAACA GAACAAGAAC AGCAGGGAAC AAGTCCACCA GCCTCCTGGG 
GGCCTGTGCC AGCCCGGACC AGTGTCACTC TTCCACCCCT TGAAGGCCAC TTTGGAGCAA 
ACCCAGCCCT CGGCATTCAG CAGTGCGAAG GCATTCCTGC GCCAGAAAAG CTCGGCCTCA 
CTCAACAATG GAGGCACATT GCCAGCCCCA GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG 
GGGACCCAAC GGACCCAGGA CGTGTTTTAT GAAACCAGGA CACCAGCTTT TGAACGAGGC 
CACTCAGAGG AAATCCCCAT TGAACGGGTG GTGGAAATGA GGGAAATCAG CCCCACACTT 
GCCAATGGAG AGCACCAACC AGGGCTGGAG AGCCAGGGTC GGGCGGCTTC CATGCCGCGC 
CTGGCTGCTG AAACGCAGCC CATCCCGGAC ACAAGCCCCA TGAAGCGCTC CATCTCCACG 
CTGACCCCGC AGCGTCCTCA CCCCATGCAC CTCTATGAGT ACTCCCTGGA GCGCGTGCCC 
ACGGACCAGG TGCATCACCA CCACCACCAC CGCTGCCACC GGCGGAAAGA GAAGAAGCAG 
AAGTCCTTGG ACAGGACCAC ACATCAGCTG GCCGATGGGG AAGCTGTGGC CCAGTCTGGT 
GAATCTTCTT CCAAGGACAA GAAGCAAGAG CGTGGACGGT CCCAAGAGAG GAAGCAGCAC 
TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC TACTCCTGTG ATCGCTATGG CAGCCGGGAC 
CGATCCCAGC CCAAGTCTGC TGATCAGAGC AGGCCTACTT CACCCAATGG TGGGCCTGAG 
CAAGGTCCCC ACAGACAGGG CAGTGGTTCG GTTAACGGGA GCCCCTTGCT GTCGACATCT 
GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG CAGCTCCCGC AGACCCCCCT GACCCCACGC 
CCCAGCATCA CTTACAAGAC CGCCAACTCC TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC 
GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG AGCCGCGGGC TGTCTGAGCA CAATGCACTG 
CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC GCGGTTGCCC GCATCGGCTC CGACCCTTAC 
CTGGGCCACC GCGACGCCGC CGACAGCCCC ATAGGCGCAG CGCCCGAGGA CACGCTCACC 
TTCGAGGAAG CCGTGGCCAC CAACTCGGGG CGTTCCTCCA GGACTTCTTA CGTCTCCTCC 
TTGACCTCCC AGTCTCACCA AGCACGCCGG GTGCCCAATG GCTACCATTA CACGCTGGGG 
CTCAACACGG GGCCCGGCAC TGGCACCAGG GGACGGAGCT ACTACCACGA AGCCGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134751.2
CDS	422..7516
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279265.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279265.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAT ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACAACTTC 
ATCAACCTCA GCTTCCTGCG GCTCTTCAGG GCAGCAAGAC TTATCAAGCT CCTTCGCCAG 
GGTTACACTA TCCGCATCTT GCTCTGGACG TTTGTGCAGT CATTTAAGGC CTTGCCTTAT 
GTGTGTCTTC TCATTGCAAT GCTCTTCTTC ATCTATGCCA TTATTGGCAT GCAGGTATTT 
GGAAACATTG CACTAAATGA CGAAACCTCC ATCAATCGGC ACAATAATTT CCGTACCTTC 
CTTCAAGCCT TGATGCTTCT GTTCAGGAGT GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCATGCC TGAGCAACAG GGCCTGCGAC CCACTCTCCG GCCTCACCAA GAACGAGTGC 
GGCAGCGACT TCGCCTACTT CTACTTCGTG TCATTCATCT TCCTCTGCTC CTTCCTGATG 
TTGAACCTAT TTGTGGCTGT AATCATGGAC AACTTTGAAT ACTTGACAAG AGACTCCTCC 
ATCCTTGGGC CCCATCATTT GGATGAGTTT GTCCGTGTCT GGGCTGAATA CGACCCAGCT 
GCCTGCGGGC GCATCAGTTA CACAGACATG TATGAGATGC TGAGACACAT GTCCCCACCT 
CTAGGCCTTG GAAAGAAATG CCCTGCTCGC GTTGCCTATA AGCGCCTGGT GCGGATGAAC 
ATGCCCATCT CACCCGAGGA TCTGACAGTC CACTTCACCT CCACGCTGAT GGCCCTGATC 
CGCACTGCTC TGGAAATCAA GCTCGCCTCA GGGGGTGTTA AGCAGCACCA GTGTGATGCT 
GAGCTGAGAA AGGAGATATC TCTAGTTTGG CCCAACCTGT CCCAGAAGAC ACTGGATTTG 
CTGGTGCCTC CTCACAAACC TGATGAGATG ACTGTGGGGA AGGTCTACGC AGCGCTCATG 
ATATTTGACT TCTACAAACA GAACAAGAAC AGCAGGGAAC AAGTCCACCA GCCTCCTGGG 
GGCCTGTGCC AGCCCGGACC AGTGTCACTC TTCCACCCCT TGAAGGCCAC TTTGGAGCAA 
ACCCAGCCCT CGGCATTCAG CAGTGCGAAG GCATTCCTGC GCCAGAAAAG CTCGGCCTCA 
CTCAACAATG GAGGCACATT GCCAGCCCCA GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG 
GGGACCCAAC GGACCCAGGA CGTGTTTTAT GAAACCAGGA CACCAGCTTT TGAACGAGGC 
CACTCAGAGG AAATCCCCAT TGAACGGGTG GTGGAAATGA GGGAAATCAG CCCCACACTT 
GCCAATGGAG AGCACCAACC AGGGCTGGAG AGCCAGGGTC GGGCGGCTTC CATGCCGCGC 
CTGGCTGCTG AAACGCAGCC CATCCCGGAC ACAAGCCCCA TGAAGCGCTC CATCTCCACG 
CTGACCCCGC AGCGTCCTCA CCCCATGCAC CTCTATGAGT ACTCCCTGGA GCGCGTGCCC 
ACGGACCAGG TGCATCACCA CCACCACCAC CGCTGCCACC GGCGGAAAGA GAAGAAGCAG 
AAGTCCTTGG ACAGGACCAC ACATCAGCTG GCCGATGGGG AAGCTGTGGC CCAGTCTGGT 
GAATCTTCTT CCAAGGACAA GAAGCAAGAG CGTGGACGGT CCCAAGAGAG GAAGCAGCAC 
TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC TACTCCTGTG ATCGCTATGG CAGCCGGGAC 
CGATCCCAGC CCAAGTCTGC TGATCAGAGC AGGCCTACTT CACCCAATGG TGGGCCTGAG 
CAAGGTCCCC ACAGACAGGG CAGTGGTTCG GTTAACGGGA GCCCCTTGCT GTCGACATCT 
GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG CAGCTCCCGC AGACCCCCCT GACCCCACGC 
CCCAGCATCA CTTACAAGAC CGCCAACTCC TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC 
GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG AGCCGCGGGC TGTCTGAGCA CAATGCACTG 
CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC GCGGTTGCCC GCATCGGCTC CGACCCTTAC 
CTGGGCCACC GCGACGCCGC CGACAGCCCC ATAGGCGCAG CGCCCGAGGA CACGCTCACC 
TTCGAGGAAG CCGTGGCCAC CAACTCGGGG CGTTCCTCCA GGACTTCTTA CGTCTCCTCC 
TTGACCTCCC AGTCTCACCA AGCACGCCGG GTGCCCAATG GCTACCATTA CACGCTGGGG 
CTCAACACGG GGCCCGGCAC TGGCACCAGG GGACGGAGCT ACTACCACGA AGCCGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279266.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134752.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.

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CloneID	OGa145075
Clone ID Related Accession (Same CDS sequence)	XM_015279266.2
Accession Version	XM_015279266.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAT ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACAACTTC 
ATCAACCTCA GCTTCCTGCG GCTCTTCAGG GCAGCAAGAC TTATCAAGCT CCTTCGCCAG 
GGTTACACTA TCCGCATCTT GCTCTGGACG TTTGTGCAGT CATTTAAGGC CTTGCCTTAT 
GTGTGTCTTC TCATTGCAAT GCTCTTCTTC ATCTATGCCA TTATTGGCAT GCAGGTATTT 
GGAAACATTG CACTAAATGA CGAAACCTCC ATCAATCGGC ACAATAATTT CCGTACCTTC 
CTTCAAGCCT TGATGCTTCT GTTCAGGAGT GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCATGCC TGAGCAACAG GGCCTGCGAC CCACTCTCCG GCCTCACCAA GAACGAGTGC 
GGCAGCGACT TCGCCTACTT CTACTTCGTG TCATTCATCT TCCTCTGCTC CTTCCTGATG 
TTGAACCTAT TTGTGGCTGT AATCATGGAC AACTTTGAAT ACTTGACAAG AGACTCCTCC 
ATCCTTGGGC CCCATCATTT GGATGAGTTT GTCCGTGTCT GGGCTGAATA CGACCCAGCT 
GCCTGTTGCC GGATTCATTA TAAGGATATG TACAATTTGT TACGTGTAAT TGCTCCACCC 
CTGGGCTTAG GAAAGAAGTG CCCTCATAGG GTGGCATACA AGCGCCTGGT GCGGATGAAC 
ATGCCCATCT CACCCGAGGA TCTGACAGTC CACTTCACCT CCACGCTGAT GGCCCTGATC 
CGCACTGCTC TGGAAATCAA GCTCGCCTCA GGGGGTGTTA AGCAGCACCA GTGTGATGCT 
GAGCTGAGAA AGGAGATATC TCTAGTTTGG CCCAACCTGT CCCAGAAGAC ACTGGATTTG 
CTGGTGCCTC CTCACAAACC TGATGAGATG ACTGTGGGGA AGGTCTACGC AGCGCTCATG 
ATATTTGACT TCTACAAACA GAACAAGAAC AGCAGGGAAC AAGTCCACCA GCCTCCTGGG 
GGCCTGTGCC AGCCCGGACC AGTGTCACTC TTCCACCCCT TGAAGGCCAC TTTGGAGCAA 
ACCCAGCCCT CGGCATTCAG CAGTGCGAAG GCATTCCTGC GCCAGAAAAG CTCGGCCTCA 
CTCAACAATG GAGGCACATT GCCAGCCCCA GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG 
GGGACCCAAC GGACCCAGGA CGTGTTTTAT GAAACCAGGA CACCAGCTTT TGAACGAGGC 
CACTCAGAGG AAATCCCCAT TGAACGGGTG GTGGAAATGA GGGAAATCAG CCCCACACTT 
GCCAATGGAG AGCACCAACC AGGGCTGGAG AGCCAGGGTC GGGCGGCTTC CATGCCGCGC 
CTGGCTGCTG AAACGCAGCC CATCCCGGAC ACAAGCCCCA TGAAGCGCTC CATCTCCACG 
CTGACCCCGC AGCGTCCTCA CCCCATGCAC CTCTATGAGT ACTCCCTGGA GCGCGTGCCC 
ACGGACCAGG TGCATCACCA CCACCACCAC CGCTGCCACC GGCGGAAAGA GAAGAAGCAG 
AAGTCCTTGG ACAGGACCAC ACATCAGCTG GCCGATGGGG AAGCTGTGGC CCAGTCTGGT 
GAATCTTCTT CCAAGGACAA GAAGCAAGAG CGTGGACGGT CCCAAGAGAG GAAGCAGCAC 
TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC TACTCCTGTG ATCGCTATGG CAGCCGGGAC 
CGATCCCAGC CCAAGTCTGC TGATCAGAGC AGGCCTACTT CACCCAATGG TGGGCCTGAG 
CAAGGTCCCC ACAGACAGGG CAGTGGTTCG GTTAACGGGA GCCCCTTGCT GTCGACATCT 
GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG CAGCTCCCGC AGACCCCCCT GACCCCACGC 
CCCAGCATCA CTTACAAGAC CGCCAACTCC TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC 
GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG AGCCGCGGGC TGTCTGAGCA CAATGCACTG 
CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC GCGGTTGCCC GCATCGGCTC CGACCCTTAC 
CTGGGCCACC GCGACGCCGC CGACAGCCCC ATAGGCGCAG CGCCCGAGGA CACGCTCACC 
TTCGAGGAAG CCGTGGCCAC CAACTCGGGG CGTTCCTCCA GGACTTCTTA CGTCTCCTCC 
TTGACCTCCC AGTCTCACCA AGCACGCCGG GTGCCCAATG GCTACCATTA CACGCTGGGG 
CTCAACACGG GGCCCGGCAC TGGCACCAGG GGACGGAGCT ACTACCACGA AGCCGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134752.2
CDS	422..7516
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279266.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279266.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAT ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACAACTTC 
ATCAACCTCA GCTTCCTGCG GCTCTTCAGG GCAGCAAGAC TTATCAAGCT CCTTCGCCAG 
GGTTACACTA TCCGCATCTT GCTCTGGACG TTTGTGCAGT CATTTAAGGC CTTGCCTTAT 
GTGTGTCTTC TCATTGCAAT GCTCTTCTTC ATCTATGCCA TTATTGGCAT GCAGGTATTT 
GGAAACATTG CACTAAATGA CGAAACCTCC ATCAATCGGC ACAATAATTT CCGTACCTTC 
CTTCAAGCCT TGATGCTTCT GTTCAGGAGT GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCATGCC TGAGCAACAG GGCCTGCGAC CCACTCTCCG GCCTCACCAA GAACGAGTGC 
GGCAGCGACT TCGCCTACTT CTACTTCGTG TCATTCATCT TCCTCTGCTC CTTCCTGATG 
TTGAACCTAT TTGTGGCTGT AATCATGGAC AACTTTGAAT ACTTGACAAG AGACTCCTCC 
ATCCTTGGGC CCCATCATTT GGATGAGTTT GTCCGTGTCT GGGCTGAATA CGACCCAGCT 
GCCTGTTGCC GGATTCATTA TAAGGATATG TACAATTTGT TACGTGTAAT TGCTCCACCC 
CTGGGCTTAG GAAAGAAGTG CCCTCATAGG GTGGCATACA AGCGCCTGGT GCGGATGAAC 
ATGCCCATCT CACCCGAGGA TCTGACAGTC CACTTCACCT CCACGCTGAT GGCCCTGATC 
CGCACTGCTC TGGAAATCAA GCTCGCCTCA GGGGGTGTTA AGCAGCACCA GTGTGATGCT 
GAGCTGAGAA AGGAGATATC TCTAGTTTGG CCCAACCTGT CCCAGAAGAC ACTGGATTTG 
CTGGTGCCTC CTCACAAACC TGATGAGATG ACTGTGGGGA AGGTCTACGC AGCGCTCATG 
ATATTTGACT TCTACAAACA GAACAAGAAC AGCAGGGAAC AAGTCCACCA GCCTCCTGGG 
GGCCTGTGCC AGCCCGGACC AGTGTCACTC TTCCACCCCT TGAAGGCCAC TTTGGAGCAA 
ACCCAGCCCT CGGCATTCAG CAGTGCGAAG GCATTCCTGC GCCAGAAAAG CTCGGCCTCA 
CTCAACAATG GAGGCACATT GCCAGCCCCA GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG 
GGGACCCAAC GGACCCAGGA CGTGTTTTAT GAAACCAGGA CACCAGCTTT TGAACGAGGC 
CACTCAGAGG AAATCCCCAT TGAACGGGTG GTGGAAATGA GGGAAATCAG CCCCACACTT 
GCCAATGGAG AGCACCAACC AGGGCTGGAG AGCCAGGGTC GGGCGGCTTC CATGCCGCGC 
CTGGCTGCTG AAACGCAGCC CATCCCGGAC ACAAGCCCCA TGAAGCGCTC CATCTCCACG 
CTGACCCCGC AGCGTCCTCA CCCCATGCAC CTCTATGAGT ACTCCCTGGA GCGCGTGCCC 
ACGGACCAGG TGCATCACCA CCACCACCAC CGCTGCCACC GGCGGAAAGA GAAGAAGCAG 
AAGTCCTTGG ACAGGACCAC ACATCAGCTG GCCGATGGGG AAGCTGTGGC CCAGTCTGGT 
GAATCTTCTT CCAAGGACAA GAAGCAAGAG CGTGGACGGT CCCAAGAGAG GAAGCAGCAC 
TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC TACTCCTGTG ATCGCTATGG CAGCCGGGAC 
CGATCCCAGC CCAAGTCTGC TGATCAGAGC AGGCCTACTT CACCCAATGG TGGGCCTGAG 
CAAGGTCCCC ACAGACAGGG CAGTGGTTCG GTTAACGGGA GCCCCTTGCT GTCGACATCT 
GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG CAGCTCCCGC AGACCCCCCT GACCCCACGC 
CCCAGCATCA CTTACAAGAC CGCCAACTCC TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC 
GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG AGCCGCGGGC TGTCTGAGCA CAATGCACTG 
CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC GCGGTTGCCC GCATCGGCTC CGACCCTTAC 
CTGGGCCACC GCGACGCCGC CGACAGCCCC ATAGGCGCAG CGCCCGAGGA CACGCTCACC 
TTCGAGGAAG CCGTGGCCAC CAACTCGGGG CGTTCCTCCA GGACTTCTTA CGTCTCCTCC 
TTGACCTCCC AGTCTCACCA AGCACGCCGG GTGCCCAATG GCTACCATTA CACGCTGGGG 
CTCAACACGG GGCCCGGCAC TGGCACCAGG GGACGGAGCT ACTACCACGA AGCCGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279267.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134753.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.

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CloneID	OGa145076
Clone ID Related Accession (Same CDS sequence)	XM_015279267.2
Accession Version	XM_015279267.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCTTTTGTAA AGCAAACTCG AGGTACTGTA TCTCGCAGCT CGTCAGTCTC CAGCGTAAAT 
TCACCCAAGC AGCAGAACTC CTCCAAATCC AAGTCGGTTT GGGAGCAACG AACCAGCCAG 
ATCCGGATGC ACAACTTCCG AGCCAGCTGC GAGGCTCTGT ACAACGAACT GGACCCCGAG 
GAGCGGGTGC GCTACGCCAC GACCCTACAC ATCCGTCCAG ACATGAAGAC CCACTTGGAT 
CGGCCACTGG TGGTAGAGCC AAGGGGTGAA GGGAGGAACA ACATCAGTAA GCTGAGCCCT 
GTGGATGTGC AGGAGGTGGA GCAGACCAAA GTCAGCTCTA CCGACGGAGC AGAAGCTCCG 
CGGAAGCATC ACCGGCATCG GGATAAGGAG AAACTGGGAG AGCAAGAGAA GGGTGATGTG 
ACCAAAGATG AGAATGGGGA ATCTGGCATC AACAATAAGG AGGAGAGGCA CAGGCAGCAC 
AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG 
GGCCAGGAAG GAGGGAAGAG GCACCATCGC CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG 
GAGCACCGGA GGCACCGCAC GCACCGGCAC TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT 
GGGACCATCA ACGGGGCCAG GAGTGAGCGG CGGACCCGCC ACCGTGGAGG GTCGAGGTCT 
GGGAACCGGG AAGGAGAGCC TGGGTCCAAG GGCGAGAATG GAGAGGAGCC TCACAGACGG 
CACCGGTTCA GGAGCAGGGC GCTGTCGACG TACGATTCGG TGGAGAAGGA GAACAGGGAG 
AAGGAAGGGG AGACTGCTGA GAAGGAGCAC CAGAATCATC AGCCCAAGGA GAATCAGTGT 
GAGATTGAGG CCAGTGGGAG TGTGAGCATC CCGGTGCACA CCCTTCCCAG CACCTATCTG 
CAGAAAGTGC CAGAGCAGCC TGAAGATGCT GACAACCAGA AGAACGTGAC ACGCATGATT 
CAGCCGCCTC TGGACAAAAC CACCACTGTG AACATCCCCG TCACCATCAC CGCCCCGCCA 
GGAGAAACCA CCGTCATACC TATGAACAAC GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG 
AAGGACGTTG ATGACTTGAC GAAAAATGGG CCTAAACCAA TCTTGCCTTA CAGTTCCATG 
TTCATCCTAA GTCCTACGAA CCCGATCCGG CGTCTGTTCC ACTACATCGT CAACCTGCGC 
TACTTCGAGA TGGTCATCCT CATCGTCATC GCTCTCAGCA GCATTGCCCT GGCAGCAGAA 
GACCCCGTCC AAGCAGAATC ACCAAGGAAC GATGCTCTGA AGTACTTGGA TTATATATTC 
ACGGGTGTCT TTACCTTCGA GATGGTGATC AAGATGATTG ACCTGGGGCT GTTACTTCAC 
CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC ATCTTGGACT TCATTGTGGT CAGTGGGGCC 
TTGGTGGCAT TTGCTTTCTC GAGTTTCATG GGAGGAACCA AAGGCAAGGA CATCAACACG 
ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA CGACCACTGA AGACCATTAA ACGGCTGCCA 
AAACTAAAGG CTGTCTTTGA CTGCGTGGTG AATTCCCTGA AGAACGTCCT CAACATCCTC 
ATTGTCTATA TGCTCTTCAT GTTCATCTTT GCTGTCATTG CTGTGCAGCT CTTCAAAGGC 
AGATTCTTCT ACTGCACTGA TGAGTCGAAA GAGCTGGAGA AGGATTGCAG GGGACAATAC 
CTTGACTATG AAAAAAATGA GGTGGAGGCG CAACCCAGGG AATGGAAAAA ATATGAGTTC 
CATTACGACA ATGTGCTGTG GGCTCTGCTG ACACTTTTCA CTGTCTCCAC AGGAGAAGGG 
TGGCCAACTG TGCTGAAGCA CTCAGTGGAT GCTACCTACG AGGAACAGGG TCCCAGCCCT 
GGCTACCGAA TGGAAATGTC TATCTTTTAC GTGGTGTATT TTGTTGTCTT CCCTTTTTTC 
TTTGTGAACA TCTTTGTGGC GTTAATCATA ATCACCTTCC AAGAGCAAGG AGATAAAGTG 
ATGTCAGAAT GCAGCCTAGA GAAAAATGAG AGGGCCTGCA TTGACTTCGC CATAAGTGCC 
AAGCCACTGA CCCGCTACAT GCCTCAGAAC AAGCAGTCAT TTCAGTACAA GATGTGGAAA 
TTTGTGGTGT CCCCGCCCTT TGAGTATTTT ATCATGGTCA TGATTGCACT CAATACTATT 
GTTCTCATGA TGAAGTTCTA TGATGCTCCA GAAGCATATG AAGAAATGTT GAAATGCCTG 
AATATTGTCT TTACATCCAT GTTTTCAATG GAGTGTGTGC TGAAGATCAT TGCCTTTGGT 
GTGCTGAATT ATTTCCGAGA TGCCTGGAAT GTCTTTGATT TTGTAACAGT GTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATTGCG GACACGGACA ACTTCATCAA CCTCAGCTTC 
CTGCGGCTCT TCAGGGCAGC AAGACTTATC AAGCTCCTTC GCCAGGGTTA CACTATCCGC 
ATCTTGCTCT GGACGTTTGT GCAGTCATTT AAGGCCTTGC CTTATGTGTG TCTTCTCATT 
GCAATGCTCT TCTTCATCTA TGCCATTATT GGCATGCAGG TATTTGGAAA CATTGCACTA 
AATGACGAAA CCTCCATCAA TCGGCACAAT AATTTCCGTA CCTTCCTTCA AGCCTTGATG 
CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC TGGCACGAGA TCATGCTGTC ATGCCTGAGC 
AACAGGGCCT GCGACCCACT CTCCGGCCTC ACCAAGAACG AGTGCGGCAG CGACTTCGCC 
TACTTCTACT TCGTGTCATT CATCTTCCTC TGCTCCTTCC TGATGTTGAA CCTATTTGTG 
GCTGTAATCA TGGACAACTT TGAATACTTG ACAAGAGACT CCTCCATCCT TGGGCCCCAT 
CATTTGGATG AGTTTGTCCG TGTCTGGGCT GAATACGACC CAGCTGCCTG CGGGCGCATC 
AGTTACACAG ACATGTATGA GATGCTGAGA CACATGTCCC CACCTCTAGG CCTTGGAAAG 
AAATGCCCTG CTCGCGTTGC CTATAAGCGC CTGGTGCGGA TGAACATGCC CATCTCACCC 
GAGGATCTGA CAGTCCACTT CACCTCCACG CTGATGGCCC TGATCCGCAC TGCTCTGGAA 
ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG CACCAGTGTG ATGCTGAGCT GAGAAAGGAG 
ATATCTCTAG TTTGGCCCAA CCTGTCCCAG AAGACACTGG ATTTGCTGGT GCCTCCTCAC 
AAACCTGATG AGATGACTGT GGGGAAGGTC TACGCAGCGC TCATGATATT TGACTTCTAC 
AAACAGAACA AGAACAGCAG GGAACAAGTC CACCAGCCTC CTGGGGGCCT GTGCCAGCCC 
GGACCAGTGT CACTCTTCCA CCCCTTGAAG GCCACTTTGG AGCAAACCCA GCCCTCGGCA 
TTCAGCAGTG CGAAGGCATT CCTGCGCCAG AAAAGCTCGG CCTCACTCAA CAATGGAGGC 
ACATTGCCAG CCCCAGAGGG TGGGATCAAA GAGTCCAGTT CGTGGGGGAC CCAACGGACC 
CAGGACGTGT TTTATGAAAC CAGGACACCA GCTTTTGAAC GAGGCCACTC AGAGGAAATC 
CCCATTGAAC GGGTGGTGGA AATGAGGGAA ATCAGCCCCA CACTTGCCAA TGGAGAGCAC 
CAACCAGGGC TGGAGAGCCA GGGTCGGGCG GCTTCCATGC CGCGCCTGGC TGCTGAAACG 
CAGCCCATCC CGGACACAAG CCCCATGAAG CGCTCCATCT CCACGCTGAC CCCGCAGCGT 
CCTCACCCCA TGCACCTCTA TGAGTACTCC CTGGAGCGCG TGCCCACGGA CCAGGTGCAT 
CACCACCACC ACCACCGCTG CCACCGGCGG AAAGAGAAGA AGCAGAAGTC CTTGGACAGG 
ACCACACATC AGCTGGCCGA TGGGGAAGCT GTGGCCCAGT CTGGTGAATC TTCTTCCAAG 
GACAAGAAGC AAGAGCGTGG ACGGTCCCAA GAGAGGAAGC AGCACTCTTC CTCCTCCTCA 
GAAAAGCAGC GCTTCTACTC CTGTGATCGC TATGGCAGCC GGGACCGATC CCAGCCCAAG 
TCTGCTGATC AGAGCAGGCC TACTTCACCC AATGGTGGGC CTGAGCAAGG TCCCCACAGA 
CAGGGCAGTG GTTCGGTTAA CGGGAGCCCC TTGCTGTCGA CATCTGGTGC TAGTACCCCC 
TGCCGGGGTC GGAGGCAGCT CCCGCAGACC CCCCTGACCC CACGCCCCAG CATCACTTAC 
AAGACCGCCA ACTCCTCGCC CGTACACTTC AGCACGTCGC CGGGCGGCCT CCCGCCCTTC 
TCCCCGGGCC GCCTGAGCCG CGGGCTGTCT GAGCACAATG CACTGCTGCG TGGGGACCAG 
CAGCCGCCCC CGGCCGCGGT TGCCCGCATC GGCTCCGACC CTTACCTGGG CCACCGCGAC 
GCCGCCGACA GCCCCATAGG CGCAGCGCCC GAGGACACGC TCACCTTCGA GGAAGCCGTG 
GCCACCAACT CGGGGCGTTC CTCCAGGACT TCTTACGTCT CCTCCTTGAC CTCCCAGTCT 
CACCAAGCAC GCCGGGTGCC CAATGGCTAC CATTACACGC TGGGGCTCAA CACGGGGCCC 
GGCACTGGCA CCAGGGGACG GAGCTACTAC CACGAAGCCG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134753.2
CDS	422..7501
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279267.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279267.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCTTTTGTAA AGCAAACTCG AGGTACTGTA TCTCGCAGCT CGTCAGTCTC CAGCGTAAAT 
TCACCCAAGC AGCAGAACTC CTCCAAATCC AAGTCGGTTT GGGAGCAACG AACCAGCCAG 
ATCCGGATGC ACAACTTCCG AGCCAGCTGC GAGGCTCTGT ACAACGAACT GGACCCCGAG 
GAGCGGGTGC GCTACGCCAC GACCCTACAC ATCCGTCCAG ACATGAAGAC CCACTTGGAT 
CGGCCACTGG TGGTAGAGCC AAGGGGTGAA GGGAGGAACA ACATCAGTAA GCTGAGCCCT 
GTGGATGTGC AGGAGGTGGA GCAGACCAAA GTCAGCTCTA CCGACGGAGC AGAAGCTCCG 
CGGAAGCATC ACCGGCATCG GGATAAGGAG AAACTGGGAG AGCAAGAGAA GGGTGATGTG 
ACCAAAGATG AGAATGGGGA ATCTGGCATC AACAATAAGG AGGAGAGGCA CAGGCAGCAC 
AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG 
GGCCAGGAAG GAGGGAAGAG GCACCATCGC CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG 
GAGCACCGGA GGCACCGCAC GCACCGGCAC TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT 
GGGACCATCA ACGGGGCCAG GAGTGAGCGG CGGACCCGCC ACCGTGGAGG GTCGAGGTCT 
GGGAACCGGG AAGGAGAGCC TGGGTCCAAG GGCGAGAATG GAGAGGAGCC TCACAGACGG 
CACCGGTTCA GGAGCAGGGC GCTGTCGACG TACGATTCGG TGGAGAAGGA GAACAGGGAG 
AAGGAAGGGG AGACTGCTGA GAAGGAGCAC CAGAATCATC AGCCCAAGGA GAATCAGTGT 
GAGATTGAGG CCAGTGGGAG TGTGAGCATC CCGGTGCACA CCCTTCCCAG CACCTATCTG 
CAGAAAGTGC CAGAGCAGCC TGAAGATGCT GACAACCAGA AGAACGTGAC ACGCATGATT 
CAGCCGCCTC TGGACAAAAC CACCACTGTG AACATCCCCG TCACCATCAC CGCCCCGCCA 
GGAGAAACCA CCGTCATACC TATGAACAAC GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG 
AAGGACGTTG ATGACTTGAC GAAAAATGGG CCTAAACCAA TCTTGCCTTA CAGTTCCATG 
TTCATCCTAA GTCCTACGAA CCCGATCCGG CGTCTGTTCC ACTACATCGT CAACCTGCGC 
TACTTCGAGA TGGTCATCCT CATCGTCATC GCTCTCAGCA GCATTGCCCT GGCAGCAGAA 
GACCCCGTCC AAGCAGAATC ACCAAGGAAC GATGCTCTGA AGTACTTGGA TTATATATTC 
ACGGGTGTCT TTACCTTCGA GATGGTGATC AAGATGATTG ACCTGGGGCT GTTACTTCAC 
CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC ATCTTGGACT TCATTGTGGT CAGTGGGGCC 
TTGGTGGCAT TTGCTTTCTC GAGTTTCATG GGAGGAACCA AAGGCAAGGA CATCAACACG 
ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA CGACCACTGA AGACCATTAA ACGGCTGCCA 
AAACTAAAGG CTGTCTTTGA CTGCGTGGTG AATTCCCTGA AGAACGTCCT CAACATCCTC 
ATTGTCTATA TGCTCTTCAT GTTCATCTTT GCTGTCATTG CTGTGCAGCT CTTCAAAGGC 
AGATTCTTCT ACTGCACTGA TGAGTCGAAA GAGCTGGAGA AGGATTGCAG GGGACAATAC 
CTTGACTATG AAAAAAATGA GGTGGAGGCG CAACCCAGGG AATGGAAAAA ATATGAGTTC 
CATTACGACA ATGTGCTGTG GGCTCTGCTG ACACTTTTCA CTGTCTCCAC AGGAGAAGGG 
TGGCCAACTG TGCTGAAGCA CTCAGTGGAT GCTACCTACG AGGAACAGGG TCCCAGCCCT 
GGCTACCGAA TGGAAATGTC TATCTTTTAC GTGGTGTATT TTGTTGTCTT CCCTTTTTTC 
TTTGTGAACA TCTTTGTGGC GTTAATCATA ATCACCTTCC AAGAGCAAGG AGATAAAGTG 
ATGTCAGAAT GCAGCCTAGA GAAAAATGAG AGGGCCTGCA TTGACTTCGC CATAAGTGCC 
AAGCCACTGA CCCGCTACAT GCCTCAGAAC AAGCAGTCAT TTCAGTACAA GATGTGGAAA 
TTTGTGGTGT CCCCGCCCTT TGAGTATTTT ATCATGGTCA TGATTGCACT CAATACTATT 
GTTCTCATGA TGAAGTTCTA TGATGCTCCA GAAGCATATG AAGAAATGTT GAAATGCCTG 
AATATTGTCT TTACATCCAT GTTTTCAATG GAGTGTGTGC TGAAGATCAT TGCCTTTGGT 
GTGCTGAATT ATTTCCGAGA TGCCTGGAAT GTCTTTGATT TTGTAACAGT GTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATTGCG GACACGGACA ACTTCATCAA CCTCAGCTTC 
CTGCGGCTCT TCAGGGCAGC AAGACTTATC AAGCTCCTTC GCCAGGGTTA CACTATCCGC 
ATCTTGCTCT GGACGTTTGT GCAGTCATTT AAGGCCTTGC CTTATGTGTG TCTTCTCATT 
GCAATGCTCT TCTTCATCTA TGCCATTATT GGCATGCAGG TATTTGGAAA CATTGCACTA 
AATGACGAAA CCTCCATCAA TCGGCACAAT AATTTCCGTA CCTTCCTTCA AGCCTTGATG 
CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC TGGCACGAGA TCATGCTGTC ATGCCTGAGC 
AACAGGGCCT GCGACCCACT CTCCGGCCTC ACCAAGAACG AGTGCGGCAG CGACTTCGCC 
TACTTCTACT TCGTGTCATT CATCTTCCTC TGCTCCTTCC TGATGTTGAA CCTATTTGTG 
GCTGTAATCA TGGACAACTT TGAATACTTG ACAAGAGACT CCTCCATCCT TGGGCCCCAT 
CATTTGGATG AGTTTGTCCG TGTCTGGGCT GAATACGACC CAGCTGCCTG CGGGCGCATC 
AGTTACACAG ACATGTATGA GATGCTGAGA CACATGTCCC CACCTCTAGG CCTTGGAAAG 
AAATGCCCTG CTCGCGTTGC CTATAAGCGC CTGGTGCGGA TGAACATGCC CATCTCACCC 
GAGGATCTGA CAGTCCACTT CACCTCCACG CTGATGGCCC TGATCCGCAC TGCTCTGGAA 
ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG CACCAGTGTG ATGCTGAGCT GAGAAAGGAG 
ATATCTCTAG TTTGGCCCAA CCTGTCCCAG AAGACACTGG ATTTGCTGGT GCCTCCTCAC 
AAACCTGATG AGATGACTGT GGGGAAGGTC TACGCAGCGC TCATGATATT TGACTTCTAC 
AAACAGAACA AGAACAGCAG GGAACAAGTC CACCAGCCTC CTGGGGGCCT GTGCCAGCCC 
GGACCAGTGT CACTCTTCCA CCCCTTGAAG GCCACTTTGG AGCAAACCCA GCCCTCGGCA 
TTCAGCAGTG CGAAGGCATT CCTGCGCCAG AAAAGCTCGG CCTCACTCAA CAATGGAGGC 
ACATTGCCAG CCCCAGAGGG TGGGATCAAA GAGTCCAGTT CGTGGGGGAC CCAACGGACC 
CAGGACGTGT TTTATGAAAC CAGGACACCA GCTTTTGAAC GAGGCCACTC AGAGGAAATC 
CCCATTGAAC GGGTGGTGGA AATGAGGGAA ATCAGCCCCA CACTTGCCAA TGGAGAGCAC 
CAACCAGGGC TGGAGAGCCA GGGTCGGGCG GCTTCCATGC CGCGCCTGGC TGCTGAAACG 
CAGCCCATCC CGGACACAAG CCCCATGAAG CGCTCCATCT CCACGCTGAC CCCGCAGCGT 
CCTCACCCCA TGCACCTCTA TGAGTACTCC CTGGAGCGCG TGCCCACGGA CCAGGTGCAT 
CACCACCACC ACCACCGCTG CCACCGGCGG AAAGAGAAGA AGCAGAAGTC CTTGGACAGG 
ACCACACATC AGCTGGCCGA TGGGGAAGCT GTGGCCCAGT CTGGTGAATC TTCTTCCAAG 
GACAAGAAGC AAGAGCGTGG ACGGTCCCAA GAGAGGAAGC AGCACTCTTC CTCCTCCTCA 
GAAAAGCAGC GCTTCTACTC CTGTGATCGC TATGGCAGCC GGGACCGATC CCAGCCCAAG 
TCTGCTGATC AGAGCAGGCC TACTTCACCC AATGGTGGGC CTGAGCAAGG TCCCCACAGA 
CAGGGCAGTG GTTCGGTTAA CGGGAGCCCC TTGCTGTCGA CATCTGGTGC TAGTACCCCC 
TGCCGGGGTC GGAGGCAGCT CCCGCAGACC CCCCTGACCC CACGCCCCAG CATCACTTAC 
AAGACCGCCA ACTCCTCGCC CGTACACTTC AGCACGTCGC CGGGCGGCCT CCCGCCCTTC 
TCCCCGGGCC GCCTGAGCCG CGGGCTGTCT GAGCACAATG CACTGCTGCG TGGGGACCAG 
CAGCCGCCCC CGGCCGCGGT TGCCCGCATC GGCTCCGACC CTTACCTGGG CCACCGCGAC 
GCCGCCGACA GCCCCATAGG CGCAGCGCCC GAGGACACGC TCACCTTCGA GGAAGCCGTG 
GCCACCAACT CGGGGCGTTC CTCCAGGACT TCTTACGTCT CCTCCTTGAC CTCCCAGTCT 
CACCAAGCAC GCCGGGTGCC CAATGGCTAC CATTACACGC TGGGGCTCAA CACGGGGCCC 
GGCACTGGCA CCAGGGGACG GAGCTACTAC CACGAAGCCG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_025156019.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025011787.1 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X9, mRNA.

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CloneID	OGa145077
Clone ID Related Accession (Same CDS sequence)	XM_025156019.1
Accession Version	XM_025156019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC GAGTTTCATG 
GGAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTATA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACAACTTCA TCAACCTCAG CTTCCTGCGG CTCTTCAGGG CAGCAAGACT TATCAAGCTC 
CTTCGCCAGG GTTACACTAT CCGCATCTTG CTCTGGACGT TTGTGCAGTC ATTTAAGGCC 
TTGCCTTATG TGTGTCTTCT CATTGCAATG CTCTTCTTCA TCTATGCCAT TATTGGCATG 
CAGGTATTTG GAAACATTGC ACTAAATGAC GAAACCTCCA TCAATCGGCA CAATAATTTC 
CGTACCTTCC TTCAAGCCTT GATGCTTCTG TTCAGGAGTG CCACGGGGGA GGCCTGGCAC 
GAGATCATGC TGTCATGCCT GAGCAACAGG GCCTGCGACC CACTCTCCGG CCTCACCAAG 
AACGAGTGCG GCAGCGACTT CGCCTACTTC TACTTCGTGT CATTCATCTT CCTCTGCTCC 
TTCCTGATGT TGAACCTATT TGTGGCTGTA ATCATGGACA ACTTTGAATA CTTGACAAGA 
GACTCCTCCA TCCTTGGGCC CCATCATTTG GATGAGTTTG TCCGTGTCTG GGCTGAATAC 
GACCCAGCTG CCTGCGGGCG CATCAGTTAC ACAGACATGT ATGAGATGCT GAGACACATG 
TCCCCACCTC TAGGCCTTGG AAAGAAATGC CCTGCTCGCG TTGCCTATAA GCGCCTGGTG 
CGGATGAACA TGCCCATCTC ACCCGAGGAT CTGACAGTCC ACTTCACCTC CACGCTGATG 
GCCCTGATCC GCACTGCTCT GGAAATCAAG CTCGCCTCAG GGGGTGTTAA GCAGCACCAG 
TGTGATGCTG AGCTGAGAAA GGAGATATCT CTAGTTTGGC CCAACCTGTC CCAGAAGACA 
CTGGATTTGC TGGTGCCTCC TCACAAACCT GATGAGATGA CTGTGGGGAA GGTCTACGCA 
GCGCTCATGA TATTTGACTT CTACAAACAG AACAAGAACA GCAGGGAACA AGTCCACCAG 
CCTCCTGGGG GCCTGTGCCA GCCCGGACCA GTGTCACTCT TCCACCCCTT GAAGGCCACT 
TTGGAGCAAA CCCAGCCCTC GGCATTCAGC AGTGCGAAGG CATTCCTGCG CCAGAAAAGC 
TCGGCCTCAC TCAACAATGG AGGCACATTG CCAGCCCCAG AGGGTGGGAT CAAAGAGTCC 
AGTTCGTGGG GGACCCAACG GACCCAGGAC GTGTTTTATG AAACCAGGAC ACCAGCTTTT 
GAACGAGGCC ACTCAGAGGA AATCCCCATT GAACGGGTGG TGGAAATGAG GGAAATCAGC 
CCCACACTTG CCAATGGAGA GCACCAACCA GGGCTGGAGA GCCAGGGTCG GGCGGCTTCC 
ATGCCGCGCC TGGCTGCTGA AACGCAGCCC ATCCCGGACA CAAGCCCCAT GAAGCGCTCC 
ATCTCCACGC TGACCCCGCA GCGTCCTCAC CCCATGCACC TCTATGAGTA CTCCCTGGAG 
CGCGTGCCCA CGGACCAGGT GCATCACCAC CACCACCACC GCTGCCACCG GCGGAAAGAG 
AAGAAGCAGA AGTCCTTGGA CAGGACCACA CATCAGCTGG CCGATGGGGA AGCTGTGGCC 
CAGTCTGGTG AATCTTCTTC CAAGGACAAG AAGCAAGAGC GTGGACGGTC CCAAGAGAGG 
AAGCAGCACT CTTCCTCCTC CTCAGAAAAG CAGCGCTTCT ACTCCTGTGA TCGCTATGGC 
AGCCGGGACC GATCCCAGCC CAAGTCTGCT GATCAGAGCA GGCCTACTTC ACCCAATGGT 
GGGCCTGAGC AAGGTCCCCA CAGACAGGGC AGTGGTTCGG TTAACGGGAG CCCCTTGCTG 
TCGACATCTG GTGCTAGTAC CCCCTGCCGG GGTCGGAGGC AGCTCCCGCA GACCCCCCTG 
ACCCCACGCC CCAGCATCAC TTACAAGACC GCCAACTCCT CGCCCGTACA CTTCAGCACG 
TCGCCGGGCG GCCTCCCGCC CTTCTCCCCG GGCCGCCTGA GCCGCGGGCT GTCTGAGCAC 
AATGCACTGC TGCGTGGGGA CCAGCAGCCG CCCCCGGCCG CGGTTGCCCG CATCGGCTCC 
GACCCTTACC TGGGCCACCG CGACGCCGCC GACAGCCCCA TAGGCGCAGC GCCCGAGGAC 
ACGCTCACCT TCGAGGAAGC CGTGGCCACC AACTCGGGGC GTTCCTCCAG GACTTCTTAC 
GTCTCCTCCT TGACCTCCCA GTCTCACCAA GCACGCCGGG TGCCCAATGG CTACCATTAC 
ACGCTGGGGC TCAACACGGG GCCCGGCACT GGCACCAGGG GACGGAGCTA CTACCACGAA 
GCCGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025011787.1
CDS	421..7464
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_025156019.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025156019.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC GAGTTTCATG 
GGAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTATA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACAACTTCA TCAACCTCAG CTTCCTGCGG CTCTTCAGGG CAGCAAGACT TATCAAGCTC 
CTTCGCCAGG GTTACACTAT CCGCATCTTG CTCTGGACGT TTGTGCAGTC ATTTAAGGCC 
TTGCCTTATG TGTGTCTTCT CATTGCAATG CTCTTCTTCA TCTATGCCAT TATTGGCATG 
CAGGTATTTG GAAACATTGC ACTAAATGAC GAAACCTCCA TCAATCGGCA CAATAATTTC 
CGTACCTTCC TTCAAGCCTT GATGCTTCTG TTCAGGAGTG CCACGGGGGA GGCCTGGCAC 
GAGATCATGC TGTCATGCCT GAGCAACAGG GCCTGCGACC CACTCTCCGG CCTCACCAAG 
AACGAGTGCG GCAGCGACTT CGCCTACTTC TACTTCGTGT CATTCATCTT CCTCTGCTCC 
TTCCTGATGT TGAACCTATT TGTGGCTGTA ATCATGGACA ACTTTGAATA CTTGACAAGA 
GACTCCTCCA TCCTTGGGCC CCATCATTTG GATGAGTTTG TCCGTGTCTG GGCTGAATAC 
GACCCAGCTG CCTGCGGGCG CATCAGTTAC ACAGACATGT ATGAGATGCT GAGACACATG 
TCCCCACCTC TAGGCCTTGG AAAGAAATGC CCTGCTCGCG TTGCCTATAA GCGCCTGGTG 
CGGATGAACA TGCCCATCTC ACCCGAGGAT CTGACAGTCC ACTTCACCTC CACGCTGATG 
GCCCTGATCC GCACTGCTCT GGAAATCAAG CTCGCCTCAG GGGGTGTTAA GCAGCACCAG 
TGTGATGCTG AGCTGAGAAA GGAGATATCT CTAGTTTGGC CCAACCTGTC CCAGAAGACA 
CTGGATTTGC TGGTGCCTCC TCACAAACCT GATGAGATGA CTGTGGGGAA GGTCTACGCA 
GCGCTCATGA TATTTGACTT CTACAAACAG AACAAGAACA GCAGGGAACA AGTCCACCAG 
CCTCCTGGGG GCCTGTGCCA GCCCGGACCA GTGTCACTCT TCCACCCCTT GAAGGCCACT 
TTGGAGCAAA CCCAGCCCTC GGCATTCAGC AGTGCGAAGG CATTCCTGCG CCAGAAAAGC 
TCGGCCTCAC TCAACAATGG AGGCACATTG CCAGCCCCAG AGGGTGGGAT CAAAGAGTCC 
AGTTCGTGGG GGACCCAACG GACCCAGGAC GTGTTTTATG AAACCAGGAC ACCAGCTTTT 
GAACGAGGCC ACTCAGAGGA AATCCCCATT GAACGGGTGG TGGAAATGAG GGAAATCAGC 
CCCACACTTG CCAATGGAGA GCACCAACCA GGGCTGGAGA GCCAGGGTCG GGCGGCTTCC 
ATGCCGCGCC TGGCTGCTGA AACGCAGCCC ATCCCGGACA CAAGCCCCAT GAAGCGCTCC 
ATCTCCACGC TGACCCCGCA GCGTCCTCAC CCCATGCACC TCTATGAGTA CTCCCTGGAG 
CGCGTGCCCA CGGACCAGGT GCATCACCAC CACCACCACC GCTGCCACCG GCGGAAAGAG 
AAGAAGCAGA AGTCCTTGGA CAGGACCACA CATCAGCTGG CCGATGGGGA AGCTGTGGCC 
CAGTCTGGTG AATCTTCTTC CAAGGACAAG AAGCAAGAGC GTGGACGGTC CCAAGAGAGG 
AAGCAGCACT CTTCCTCCTC CTCAGAAAAG CAGCGCTTCT ACTCCTGTGA TCGCTATGGC 
AGCCGGGACC GATCCCAGCC CAAGTCTGCT GATCAGAGCA GGCCTACTTC ACCCAATGGT 
GGGCCTGAGC AAGGTCCCCA CAGACAGGGC AGTGGTTCGG TTAACGGGAG CCCCTTGCTG 
TCGACATCTG GTGCTAGTAC CCCCTGCCGG GGTCGGAGGC AGCTCCCGCA GACCCCCCTG 
ACCCCACGCC CCAGCATCAC TTACAAGACC GCCAACTCCT CGCCCGTACA CTTCAGCACG 
TCGCCGGGCG GCCTCCCGCC CTTCTCCCCG GGCCGCCTGA GCCGCGGGCT GTCTGAGCAC 
AATGCACTGC TGCGTGGGGA CCAGCAGCCG CCCCCGGCCG CGGTTGCCCG CATCGGCTCC 
GACCCTTACC TGGGCCACCG CGACGCCGCC GACAGCCCCA TAGGCGCAGC GCCCGAGGAC 
ACGCTCACCT TCGAGGAAGC CGTGGCCACC AACTCGGGGC GTTCCTCCAG GACTTCTTAC 
GTCTCCTCCT TGACCTCCCA GTCTCACCAA GCACGCCGGG TGCCCAATGG CTACCATTAC 
ACGCTGGGGC TCAACACGGG GCCCGGCACT GGCACCAGGG GACGGAGCTA CTACCACGAA 
GCCGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279268.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134754.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.

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CloneID	OGa145078
Clone ID Related Accession (Same CDS sequence)	XM_015279268.2
Accession Version	XM_015279268.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCCAAGCAGC AGAACTCCTC CAAATCCAAG TCGGTTTGGG AGCAACGAAC CAGCCAGATC 
CGGATGCACA ACTTCCGAGC CAGCTGCGAG GCTCTGTACA ACGAACTGGA CCCCGAGGAG 
CGGGTGCGCT ACGCCACGAC CCTACACATC CGTCCAGACA TGAAGACCCA CTTGGATCGG 
CCACTGGTGG TAGAGCCAAG GGGTGAAGGG AGGAACAACA TCAGTAAGCT GAGCCCTGTG 
GATGTGCAGG AGGTGGAGCA GACCAAAGTC AGCTCTACCG ACGGAGCAGA AGCTCCGCGG 
AAGCATCACC GGCATCGGGA TAAGGAGAAA CTGGGAGAGC AAGAGAAGGG TGATGTGACC 
AAAGATGAGA ATGGGGAATC TGGCATCAAC AATAAGGAGG AGAGGCACAG GCAGCACAGA 
AGCCGTAGCA AGGAGGTGGA AGGGGGCAGC AAGGAAGGGA AAAGTGACCG CAGCAGGGGC 
CAGGAAGGAG GGAAGAGGCA CCATCGCCGG GGGTCGGTGG AAGAAGGCGT TGAGAAGGAG 
CACCGGAGGC ACCGCACGCA CCGGCACTCT GCAGAACGGC AAGGCAAGGA GGGCAATGGG 
ACCATCAACG GGGCCAGGAG TGAGCGGCGG ACCCGCCACC GTGGAGGGTC GAGGTCTGGG 
AACCGGGAAG GAGAGCCTGG GTCCAAGGGC GAGAATGGAG AGGAGCCTCA CAGACGGCAC 
CGGTTCAGGA GCAGGGCGCT GTCGACGTAC GATTCGGTGG AGAAGGAGAA CAGGGAGAAG 
GAAGGGGAGA CTGCTGAGAA GGAGCACCAG AATCATCAGC CCAAGGAGAA TCAGTGTGAG 
ATTGAGGCCA GTGGGAGTGT GAGCATCCCG GTGCACACCC TTCCCAGCAC CTATCTGCAG 
AAAGTGCCAG AGCAGCCTGA AGATGCTGAC AACCAGAAGA ACGTGACACG CATGATTCAG 
CCGCCTCTGG ACAAAACCAC CACTGTGAAC ATCCCCGTCA CCATCACCGC CCCGCCAGGA 
GAAACCACCG TCATACCTAT GAACAACGTT GAGTTCGAAA GTAAAACAGA AGAGAAGAAG 
GACGTTGATG ACTTGACGAA AAATGGGCCT AAACCAATCT TGCCTTACAG TTCCATGTTC 
ATCCTAAGTC CTACGAACCC GATCCGGCGT CTGTTCCACT ACATCGTCAA CCTGCGCTAC 
TTCGAGATGG TCATCCTCAT CGTCATCGCT CTCAGCAGCA TTGCCCTGGC AGCAGAAGAC 
CCCGTCCAAG CAGAATCACC AAGGAACGAT GCTCTGAAGT ACTTGGATTA TATATTCACG 
GGTGTCTTTA CCTTCGAGAT GGTGATCAAG ATGATTGACC TGGGGCTGTT ACTTCACCCT 
GGCTCCTATT TCCGTGACCT GTGGAACATC TTGGACTTCA TTGTGGTCAG TGGGGCCTTG 
GTGGCATTTG CTTTCTCAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTATATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGACT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGTTGCC GGATTCATTA TAAGGATATG 
TACAATTTGT TACGTGTAAT TGCTCCACCC CTGGGCTTAG GAAAGAAGTG CCCTCATAGG 
GTGGCATACA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGAG GAGCAAAGCA 
CGGTCACCTG GGAGTTACCT AGCACCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGGGCAGT GGTTCGGTTA ACGGGAGCCC CTTGCTGTCG 
ACATCTGGTG CTAGTACCCC CTGCCGGGGT CGGAGGCAGC TCCCGCAGAC CCCCCTGACC 
CCACGCCCCA GCATCACTTA CAAGACCGCC AACTCCTCGC CCGTACACTT CAGCACGTCG 
CCGGGCGGCC TCCCGCCCTT CTCCCCGGGC CGCCTGAGCC GCGGGCTGTC TGAGCACAAT 
GCACTGCTGC GTGGGGACCA GCAGCCGCCC CCGGCCGCGG TTGCCCGCAT CGGCTCCGAC 
CCTTACCTGG GCCACCGCGA CGCCGCCGAC AGCCCCATAG GCGCAGCGCC CGAGGACACG 
CTCACCTTCG AGGAAGCCGT GGCCACCAAC TCGGGGCGTT CCTCCAGGAC TTCTTACGTC 
TCCTCCTTGA CCTCCCAGTC TCACCAAGCA CGCCGGGTGC CCAATGGCTA CCATTACACG 
CTGGGGCTCA ACACGGGGCC CGGCACTGGC ACCAGGGGAC GGAGCTACTA CCACGAAGCC 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134754.2
CDS	422..7462
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279268.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279268.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCCAAGCAGC AGAACTCCTC CAAATCCAAG TCGGTTTGGG AGCAACGAAC CAGCCAGATC 
CGGATGCACA ACTTCCGAGC CAGCTGCGAG GCTCTGTACA ACGAACTGGA CCCCGAGGAG 
CGGGTGCGCT ACGCCACGAC CCTACACATC CGTCCAGACA TGAAGACCCA CTTGGATCGG 
CCACTGGTGG TAGAGCCAAG GGGTGAAGGG AGGAACAACA TCAGTAAGCT GAGCCCTGTG 
GATGTGCAGG AGGTGGAGCA GACCAAAGTC AGCTCTACCG ACGGAGCAGA AGCTCCGCGG 
AAGCATCACC GGCATCGGGA TAAGGAGAAA CTGGGAGAGC AAGAGAAGGG TGATGTGACC 
AAAGATGAGA ATGGGGAATC TGGCATCAAC AATAAGGAGG AGAGGCACAG GCAGCACAGA 
AGCCGTAGCA AGGAGGTGGA AGGGGGCAGC AAGGAAGGGA AAAGTGACCG CAGCAGGGGC 
CAGGAAGGAG GGAAGAGGCA CCATCGCCGG GGGTCGGTGG AAGAAGGCGT TGAGAAGGAG 
CACCGGAGGC ACCGCACGCA CCGGCACTCT GCAGAACGGC AAGGCAAGGA GGGCAATGGG 
ACCATCAACG GGGCCAGGAG TGAGCGGCGG ACCCGCCACC GTGGAGGGTC GAGGTCTGGG 
AACCGGGAAG GAGAGCCTGG GTCCAAGGGC GAGAATGGAG AGGAGCCTCA CAGACGGCAC 
CGGTTCAGGA GCAGGGCGCT GTCGACGTAC GATTCGGTGG AGAAGGAGAA CAGGGAGAAG 
GAAGGGGAGA CTGCTGAGAA GGAGCACCAG AATCATCAGC CCAAGGAGAA TCAGTGTGAG 
ATTGAGGCCA GTGGGAGTGT GAGCATCCCG GTGCACACCC TTCCCAGCAC CTATCTGCAG 
AAAGTGCCAG AGCAGCCTGA AGATGCTGAC AACCAGAAGA ACGTGACACG CATGATTCAG 
CCGCCTCTGG ACAAAACCAC CACTGTGAAC ATCCCCGTCA CCATCACCGC CCCGCCAGGA 
GAAACCACCG TCATACCTAT GAACAACGTT GAGTTCGAAA GTAAAACAGA AGAGAAGAAG 
GACGTTGATG ACTTGACGAA AAATGGGCCT AAACCAATCT TGCCTTACAG TTCCATGTTC 
ATCCTAAGTC CTACGAACCC GATCCGGCGT CTGTTCCACT ACATCGTCAA CCTGCGCTAC 
TTCGAGATGG TCATCCTCAT CGTCATCGCT CTCAGCAGCA TTGCCCTGGC AGCAGAAGAC 
CCCGTCCAAG CAGAATCACC AAGGAACGAT GCTCTGAAGT ACTTGGATTA TATATTCACG 
GGTGTCTTTA CCTTCGAGAT GGTGATCAAG ATGATTGACC TGGGGCTGTT ACTTCACCCT 
GGCTCCTATT TCCGTGACCT GTGGAACATC TTGGACTTCA TTGTGGTCAG TGGGGCCTTG 
GTGGCATTTG CTTTCTCAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTATATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGACT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGTTGCC GGATTCATTA TAAGGATATG 
TACAATTTGT TACGTGTAAT TGCTCCACCC CTGGGCTTAG GAAAGAAGTG CCCTCATAGG 
GTGGCATACA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGAG GAGCAAAGCA 
CGGTCACCTG GGAGTTACCT AGCACCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGGGCAGT GGTTCGGTTA ACGGGAGCCC CTTGCTGTCG 
ACATCTGGTG CTAGTACCCC CTGCCGGGGT CGGAGGCAGC TCCCGCAGAC CCCCCTGACC 
CCACGCCCCA GCATCACTTA CAAGACCGCC AACTCCTCGC CCGTACACTT CAGCACGTCG 
CCGGGCGGCC TCCCGCCCTT CTCCCCGGGC CGCCTGAGCC GCGGGCTGTC TGAGCACAAT 
GCACTGCTGC GTGGGGACCA GCAGCCGCCC CCGGCCGCGG TTGCCCGCAT CGGCTCCGAC 
CCTTACCTGG GCCACCGCGA CGCCGCCGAC AGCCCCATAG GCGCAGCGCC CGAGGACACG 
CTCACCTTCG AGGAAGCCGT GGCCACCAAC TCGGGGCGTT CCTCCAGGAC TTCTTACGTC 
TCCTCCTTGA CCTCCCAGTC TCACCAAGCA CGCCGGGTGC CCAATGGCTA CCATTACACG 
CTGGGGCTCA ACACGGGGCC CGGCACTGGC ACCAGGGGAC GGAGCTACTA CCACGAAGCC 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279269.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134755.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.

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CloneID	OGa145079
Clone ID Related Accession (Same CDS sequence)	XM_015279269.2
Accession Version	XM_015279269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC TTTTGTAAAG CAAACTCGAG GTACTGTATC TCGCAGCTCG 
TCAGTCTCCA GCGTAAATTC ACCCAAGCAG CAGAACTCCT CCAAATCCAA GTCGGTTTGG 
GAGCAACGAA CCAGCCAGAT CCGGATGCAC AACTTCCGAG CCAGCTGCGA GGCTCTGTAC 
AACGAACTGG ACCCCGAGGA GCGGGTGCGC TACGCCACGA CCCTACACAT CCGTCCAGAC 
ATGAAGACCC ACTTGGATCG GCCACTGGTG GTAGAGCCAA GGGGTGAAGG GAGGAACAAC 
ATCAGTAAGC TGAGCCCTGT GGATGTGCAG GAGGTGGAGC AGACCAAAGT CAGCTCTACC 
GACGGAGCAG AAGCTCCGCG GAAGCATCAC CGGCATCGGG ATAAGGAGAA ACTGGGAGAG 
CAAGAGAAGG GTGATGTGAC CAAAGATGAG AATGGGGAAT CTGGCATCAA CAATAAGGAG 
GAGAGGCACA GGCAGCACAG AAGCCGTAGC AAGGAGGTGG AAGGGGGCAG CAAGGAAGGG 
AAAAGTGACC GCAGCAGGGG CCAGGAAGGA GGGAAGAGGC ACCATCGCCG GGGGTCGGTG 
GAAGAAGGCG TTGAGAAGGA GCACCGGAGG CACCGCACGC ACCGGCACTC TGCAGAACGG 
CAAGGCAAGG AGGGCAATGG GACCATCAAC GGGGCCAGGA GTGAGCGGCG GACCCGCCAC 
CGTGGAGGGT CGAGGTCTGG GAACCGGGAA GGAGAGCCTG GGTCCAAGGG CGAGAATGGA 
GAGGAGCCTC ACAGACGGCA CCGGTTCAGG AGCAGGGCGC TGTCGACGTA CGATTCGGTG 
GAGAAGGAGA ACAGGGAGAA GGAAGGGGAG ACTGCTGAGA AGGAGCACCA GAATCATCAG 
CCCAAGGAGA ATCAGTGTGA GATTGAGGCC AGTGGGAGTG TGAGCATCCC GGTGCACACC 
CTTCCCAGCA CCTATCTGCA GAAAGTGCCA GAGCAGCCTG AAGATGCTGA CAACCAGAAG 
AACGTGACAC GCATGATTCA GCCGCCTCTG GACAAAACCA CCACTGTGAA CATCCCCGTC 
ACCATCACCG CCCCGCCAGG AGAAACCACC GTCATACCTA TGAACAACGT TGAGTTCGAA 
AGTAAAACAG AAGAGAAGAA GGACGTTGAT GACTTGACGA AAAATGGGCC TAAACCAATC 
TTGCCTTACA GTTCCATGTT CATCCTAAGT CCTACGAACC CGATCCGGCG TCTGTTCCAC 
TACATCGTCA ACCTGCGCTA CTTCGAGATG GTCATCCTCA TCGTCATCGC TCTCAGCAGC 
ATTGCCCTGG CAGCAGAAGA CCCCGTCCAA GCAGAATCAC CAAGGAACGA TGCTCTGAAG 
TACTTGGATT ATATATTCAC GGGTGTCTTT ACCTTCGAGA TGGTGATCAA GATGATTGAC 
CTGGGGCTGT TACTTCACCC TGGCTCCTAT TTCCGTGACC TGTGGAACAT CTTGGACTTC 
ATTGTGGTCA GTGGGGCCTT GGTGGCATTT GCTTTCTCGA GTTTCATGGG AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTATATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG ACTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGCG GGCGCATCAG TTACACAGAC ATGTATGAGA TGCTGAGACA CATGTCCCCA 
CCTCTAGGCC TTGGAAAGAA ATGCCCTGCT CGCGTTGCCT ATAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACA GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACACTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AACAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ATGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCGGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GCCCATCCCG GACACAAGCC CCATGAAGCG CTCCATCTCC 
ACGCTGACCC CGCAGCGTCC TCACCCCATG CACCTCTATG AGTACTCCCT GGAGCGCGTG 
CCCACGGACC AGGTGCATCA CCACCACCAC CACCGCTGCC ACCGGCGGAA AGAGAAGAAG 
CAGAAGTCCT TGGACAGGAC CACACATCAG CTGGCCGATG GGGAAGCTGT GGCCCAGTCT 
GGTGAATCTT CTTCCAAGGA CAAGAAGCAA GAGCGTGGAC GGTCCCAAGA GAGGAAGCAG 
CACTCTTCCT CCTCCTCAGA AAAGCAGCGC TTCTACTCCT GTGATCGCTA TGGCAGCCGG 
GACCGATCCC AGCCCAAGTC TGCTGATCAG AGCAGGCCTA CTTCACCCAA TGGTGGGCCT 
GAGCAAGGTC CCCACAGACA GGGCAGTGGT TCGGTTAACG GGAGCCCCTT GCTGTCGACA 
TCTGGTGCTA GTACCCCCTG CCGGGGTCGG AGGCAGCTCC CGCAGACCCC CCTGACCCCA 
CGCCCCAGCA TCACTTACAA GACCGCCAAC TCCTCGCCCG TACACTTCAG CACGTCGCCG 
GGCGGCCTCC CGCCCTTCTC CCCGGGCCGC CTGAGCCGCG GGCTGTCTGA GCACAATGCA 
CTGCTGCGTG GGGACCAGCA GCCGCCCCCG GCCGCGGTTG CCCGCATCGG CTCCGACCCT 
TACCTGGGCC ACCGCGACGC CGCCGACAGC CCCATAGGCG CAGCGCCCGA GGACACGCTC 
ACCTTCGAGG AAGCCGTGGC CACCAACTCG GGGCGTTCCT CCAGGACTTC TTACGTCTCC 
TCCTTGACCT CCCAGTCTCA CCAAGCACGC CGGGTGCCCA ATGGCTACCA TTACACGCTG 
GGGCTCAACA CGGGGCCCGG CACTGGCACC AGGGGACGGA GCTACTACCA CGAAGCCGAC 
GAGGATGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134755.2
CDS	421..7458
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279269.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279269.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC TTTTGTAAAG CAAACTCGAG GTACTGTATC TCGCAGCTCG 
TCAGTCTCCA GCGTAAATTC ACCCAAGCAG CAGAACTCCT CCAAATCCAA GTCGGTTTGG 
GAGCAACGAA CCAGCCAGAT CCGGATGCAC AACTTCCGAG CCAGCTGCGA GGCTCTGTAC 
AACGAACTGG ACCCCGAGGA GCGGGTGCGC TACGCCACGA CCCTACACAT CCGTCCAGAC 
ATGAAGACCC ACTTGGATCG GCCACTGGTG GTAGAGCCAA GGGGTGAAGG GAGGAACAAC 
ATCAGTAAGC TGAGCCCTGT GGATGTGCAG GAGGTGGAGC AGACCAAAGT CAGCTCTACC 
GACGGAGCAG AAGCTCCGCG GAAGCATCAC CGGCATCGGG ATAAGGAGAA ACTGGGAGAG 
CAAGAGAAGG GTGATGTGAC CAAAGATGAG AATGGGGAAT CTGGCATCAA CAATAAGGAG 
GAGAGGCACA GGCAGCACAG AAGCCGTAGC AAGGAGGTGG AAGGGGGCAG CAAGGAAGGG 
AAAAGTGACC GCAGCAGGGG CCAGGAAGGA GGGAAGAGGC ACCATCGCCG GGGGTCGGTG 
GAAGAAGGCG TTGAGAAGGA GCACCGGAGG CACCGCACGC ACCGGCACTC TGCAGAACGG 
CAAGGCAAGG AGGGCAATGG GACCATCAAC GGGGCCAGGA GTGAGCGGCG GACCCGCCAC 
CGTGGAGGGT CGAGGTCTGG GAACCGGGAA GGAGAGCCTG GGTCCAAGGG CGAGAATGGA 
GAGGAGCCTC ACAGACGGCA CCGGTTCAGG AGCAGGGCGC TGTCGACGTA CGATTCGGTG 
GAGAAGGAGA ACAGGGAGAA GGAAGGGGAG ACTGCTGAGA AGGAGCACCA GAATCATCAG 
CCCAAGGAGA ATCAGTGTGA GATTGAGGCC AGTGGGAGTG TGAGCATCCC GGTGCACACC 
CTTCCCAGCA CCTATCTGCA GAAAGTGCCA GAGCAGCCTG AAGATGCTGA CAACCAGAAG 
AACGTGACAC GCATGATTCA GCCGCCTCTG GACAAAACCA CCACTGTGAA CATCCCCGTC 
ACCATCACCG CCCCGCCAGG AGAAACCACC GTCATACCTA TGAACAACGT TGAGTTCGAA 
AGTAAAACAG AAGAGAAGAA GGACGTTGAT GACTTGACGA AAAATGGGCC TAAACCAATC 
TTGCCTTACA GTTCCATGTT CATCCTAAGT CCTACGAACC CGATCCGGCG TCTGTTCCAC 
TACATCGTCA ACCTGCGCTA CTTCGAGATG GTCATCCTCA TCGTCATCGC TCTCAGCAGC 
ATTGCCCTGG CAGCAGAAGA CCCCGTCCAA GCAGAATCAC CAAGGAACGA TGCTCTGAAG 
TACTTGGATT ATATATTCAC GGGTGTCTTT ACCTTCGAGA TGGTGATCAA GATGATTGAC 
CTGGGGCTGT TACTTCACCC TGGCTCCTAT TTCCGTGACC TGTGGAACAT CTTGGACTTC 
ATTGTGGTCA GTGGGGCCTT GGTGGCATTT GCTTTCTCGA GTTTCATGGG AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTATATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG ACTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGCG GGCGCATCAG TTACACAGAC ATGTATGAGA TGCTGAGACA CATGTCCCCA 
CCTCTAGGCC TTGGAAAGAA ATGCCCTGCT CGCGTTGCCT ATAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACA GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACACTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AACAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ATGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCGGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GCCCATCCCG GACACAAGCC CCATGAAGCG CTCCATCTCC 
ACGCTGACCC CGCAGCGTCC TCACCCCATG CACCTCTATG AGTACTCCCT GGAGCGCGTG 
CCCACGGACC AGGTGCATCA CCACCACCAC CACCGCTGCC ACCGGCGGAA AGAGAAGAAG 
CAGAAGTCCT TGGACAGGAC CACACATCAG CTGGCCGATG GGGAAGCTGT GGCCCAGTCT 
GGTGAATCTT CTTCCAAGGA CAAGAAGCAA GAGCGTGGAC GGTCCCAAGA GAGGAAGCAG 
CACTCTTCCT CCTCCTCAGA AAAGCAGCGC TTCTACTCCT GTGATCGCTA TGGCAGCCGG 
GACCGATCCC AGCCCAAGTC TGCTGATCAG AGCAGGCCTA CTTCACCCAA TGGTGGGCCT 
GAGCAAGGTC CCCACAGACA GGGCAGTGGT TCGGTTAACG GGAGCCCCTT GCTGTCGACA 
TCTGGTGCTA GTACCCCCTG CCGGGGTCGG AGGCAGCTCC CGCAGACCCC CCTGACCCCA 
CGCCCCAGCA TCACTTACAA GACCGCCAAC TCCTCGCCCG TACACTTCAG CACGTCGCCG 
GGCGGCCTCC CGCCCTTCTC CCCGGGCCGC CTGAGCCGCG GGCTGTCTGA GCACAATGCA 
CTGCTGCGTG GGGACCAGCA GCCGCCCCCG GCCGCGGTTG CCCGCATCGG CTCCGACCCT 
TACCTGGGCC ACCGCGACGC CGCCGACAGC CCCATAGGCG CAGCGCCCGA GGACACGCTC 
ACCTTCGAGG AAGCCGTGGC CACCAACTCG GGGCGTTCCT CCAGGACTTC TTACGTCTCC 
TCCTTGACCT CCCAGTCTCA CCAAGCACGC CGGGTGCCCA ATGGCTACCA TTACACGCTG 
GGGCTCAACA CGGGGCCCGG CACTGGCACC AGGGGACGGA GCTACTACCA CGAAGCCGAC 
GAGGATGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279270.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134756.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X12, mRNA.

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CloneID	OGa145080
Clone ID Related Accession (Same CDS sequence)	XM_015279270.2
Accession Version	XM_015279270.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTATATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CAACTTCATC 
AACCTCAGCT TCCTGCGGCT CTTCAGGGCA GCAAGACTTA TCAAGCTCCT TCGCCAGGGT 
TACACTATCC GCATCTTGCT CTGGACGTTT GTGCAGTCAT TTAAGGCCTT GCCTTATGTG 
TGTCTTCTCA TTGCAATGCT CTTCTTCATC TATGCCATTA TTGGCATGCA GGTATTTGGA 
AACATTGCAC TAAATGACGA AACCTCCATC AATCGGCACA ATAATTTCCG TACCTTCCTT 
CAAGCCTTGA TGCTTCTGTT CAGGAGTGCC ACGGGGGAGG CCTGGCACGA GATCATGCTG 
TCATGCCTGA GCAACAGGGC CTGCGACCCA CTCTCCGGCC TCACCAAGAA CGAGTGCGGC 
AGCGACTTCG CCTACTTCTA CTTCGTGTCA TTCATCTTCC TCTGCTCCTT CCTGATGTTG 
AACCTATTTG TGGCTGTAAT CATGGACAAC TTTGAATACT TGACAAGAGA CTCCTCCATC 
CTTGGGCCCC ATCATTTGGA TGAGTTTGTC CGTGTCTGGG CTGAATACGA CCCAGCTGCC 
TGCGGGCGCA TCAGTTACAC AGACATGTAT GAGATGCTGA GACACATGTC CCCACCTCTA 
GGCCTTGGAA AGAAATGCCC TGCTCGCGTT GCCTATAAGC GCCTGGTGCG GATGAACATG 
CCCATCTCAC CCGAGGATCT GACAGTCCAC TTCACCTCCA CGCTGATGGC CCTGATCCGC 
ACTGCTCTGG AAATCAAGCT CGCCTCAGGG GGTGTTAAGC AGCACCAGTG TGATGCTGAG 
CTGAGAAAGG AGATATCTCT AGTTTGGCCC AACCTGTCCC AGAAGACACT GGATTTGCTG 
GTGCCTCCTC ACAAACCTGA TGAGATGACT GTGGGGAAGG TCTACGCAGC GCTCATGATA 
TTTGACTTCT ACAAACAGAA CAAGAACAGC AGGGAACAAG TCCACCAGCC TCCTGGGGGC 
CTGTGCCAGC CCGGACCAGT GTCACTCTTC CACCCCTTGA AGGCCACTTT GGAGCAAACC 
CAGCCCTCGG CATTCAGCAG TGCGAAGGCA TTCCTGCGCC AGAAAAGCTC GGCCTCACTC 
AACAATGGAG GCACATTGCC AGCCCCAGAG GGTGGGATCA AAGAGTCCAG TTCGTGGGGG 
ACCCAACGGA CCCAGGACGT GTTTTATGAA ACCAGGACAC CAGCTTTTGA ACGAGGCCAC 
TCAGAGGAAA TCCCCATTGA ACGGGTGGTG GAAATGAGGG AAATCAGCCC CACACTTGCC 
AATGGAGAGC ACCAACCAGG GCTGGAGAGC CAGGGTCGGG CGGCTTCCAT GCCGCGCCTG 
GCTGCTGAAA CGCAGCCCAT CCCGGACACA AGCCCCATGA AGCGCTCCAT CTCCACGCTG 
ACCCCGCAGC GTCCTCACCC CATGCACCTC TATGAGTACT CCCTGGAGCG CGTGCCCACG 
GACCAGGTGC ATCACCACCA CCACCACCGC TGCCACCGGC GGAAAGAGAA GAAGCAGAAG 
TCCTTGGACA GGACCACACA TCAGCTGGCC GATGGGGAAG CTGTGGCCCA GTCTGGTGAA 
TCTTCTTCCA AGGACAAGAA GCAAGAGCGT GGACGGTCCC AAGAGAGGAA GCAGCACTCT 
TCCTCCTCCT CAGAAAAGCA GCGCTTCTAC TCCTGTGATC GCTATGGCAG CCGGGACCGA 
TCCCAGCCCA AGTCTGCTGA TCAGAGCAGG CCTACTTCAC CCAATGGTGG GCCTGAGCAA 
GGTCCCCACA GACAGGGCAG TGGTTCGGTT AACGGGAGCC CCTTGCTGTC GACATCTGGT 
GCTAGTACCC CCTGCCGGGG TCGGAGGCAG CTCCCGCAGA CCCCCCTGAC CCCACGCCCC 
AGCATCACTT ACAAGACCGC CAACTCCTCG CCCGTACACT TCAGCACGTC GCCGGGCGGC 
CTCCCGCCCT TCTCCCCGGG CCGCCTGAGC CGCGGGCTGT CTGAGCACAA TGCACTGCTG 
CGTGGGGACC AGCAGCCGCC CCCGGCCGCG GTTGCCCGCA TCGGCTCCGA CCCTTACCTG 
GGCCACCGCG ACGCCGCCGA CAGCCCCATA GGCGCAGCGC CCGAGGACAC GCTCACCTTC 
GAGGAAGCCG TGGCCACCAA CTCGGGGCGT TCCTCCAGGA CTTCTTACGT CTCCTCCTTG 
ACCTCCCAGT CTCACCAAGC ACGCCGGGTG CCCAATGGCT ACCATTACAC GCTGGGGCTC 
AACACGGGGC CCGGCACTGG CACCAGGGGA CGGAGCTACT ACCACGAAGC CGACGAGGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134756.2
CDS	421..7452
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279270.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279270.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTATATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CAACTTCATC 
AACCTCAGCT TCCTGCGGCT CTTCAGGGCA GCAAGACTTA TCAAGCTCCT TCGCCAGGGT 
TACACTATCC GCATCTTGCT CTGGACGTTT GTGCAGTCAT TTAAGGCCTT GCCTTATGTG 
TGTCTTCTCA TTGCAATGCT CTTCTTCATC TATGCCATTA TTGGCATGCA GGTATTTGGA 
AACATTGCAC TAAATGACGA AACCTCCATC AATCGGCACA ATAATTTCCG TACCTTCCTT 
CAAGCCTTGA TGCTTCTGTT CAGGAGTGCC ACGGGGGAGG CCTGGCACGA GATCATGCTG 
TCATGCCTGA GCAACAGGGC CTGCGACCCA CTCTCCGGCC TCACCAAGAA CGAGTGCGGC 
AGCGACTTCG CCTACTTCTA CTTCGTGTCA TTCATCTTCC TCTGCTCCTT CCTGATGTTG 
AACCTATTTG TGGCTGTAAT CATGGACAAC TTTGAATACT TGACAAGAGA CTCCTCCATC 
CTTGGGCCCC ATCATTTGGA TGAGTTTGTC CGTGTCTGGG CTGAATACGA CCCAGCTGCC 
TGCGGGCGCA TCAGTTACAC AGACATGTAT GAGATGCTGA GACACATGTC CCCACCTCTA 
GGCCTTGGAA AGAAATGCCC TGCTCGCGTT GCCTATAAGC GCCTGGTGCG GATGAACATG 
CCCATCTCAC CCGAGGATCT GACAGTCCAC TTCACCTCCA CGCTGATGGC CCTGATCCGC 
ACTGCTCTGG AAATCAAGCT CGCCTCAGGG GGTGTTAAGC AGCACCAGTG TGATGCTGAG 
CTGAGAAAGG AGATATCTCT AGTTTGGCCC AACCTGTCCC AGAAGACACT GGATTTGCTG 
GTGCCTCCTC ACAAACCTGA TGAGATGACT GTGGGGAAGG TCTACGCAGC GCTCATGATA 
TTTGACTTCT ACAAACAGAA CAAGAACAGC AGGGAACAAG TCCACCAGCC TCCTGGGGGC 
CTGTGCCAGC CCGGACCAGT GTCACTCTTC CACCCCTTGA AGGCCACTTT GGAGCAAACC 
CAGCCCTCGG CATTCAGCAG TGCGAAGGCA TTCCTGCGCC AGAAAAGCTC GGCCTCACTC 
AACAATGGAG GCACATTGCC AGCCCCAGAG GGTGGGATCA AAGAGTCCAG TTCGTGGGGG 
ACCCAACGGA CCCAGGACGT GTTTTATGAA ACCAGGACAC CAGCTTTTGA ACGAGGCCAC 
TCAGAGGAAA TCCCCATTGA ACGGGTGGTG GAAATGAGGG AAATCAGCCC CACACTTGCC 
AATGGAGAGC ACCAACCAGG GCTGGAGAGC CAGGGTCGGG CGGCTTCCAT GCCGCGCCTG 
GCTGCTGAAA CGCAGCCCAT CCCGGACACA AGCCCCATGA AGCGCTCCAT CTCCACGCTG 
ACCCCGCAGC GTCCTCACCC CATGCACCTC TATGAGTACT CCCTGGAGCG CGTGCCCACG 
GACCAGGTGC ATCACCACCA CCACCACCGC TGCCACCGGC GGAAAGAGAA GAAGCAGAAG 
TCCTTGGACA GGACCACACA TCAGCTGGCC GATGGGGAAG CTGTGGCCCA GTCTGGTGAA 
TCTTCTTCCA AGGACAAGAA GCAAGAGCGT GGACGGTCCC AAGAGAGGAA GCAGCACTCT 
TCCTCCTCCT CAGAAAAGCA GCGCTTCTAC TCCTGTGATC GCTATGGCAG CCGGGACCGA 
TCCCAGCCCA AGTCTGCTGA TCAGAGCAGG CCTACTTCAC CCAATGGTGG GCCTGAGCAA 
GGTCCCCACA GACAGGGCAG TGGTTCGGTT AACGGGAGCC CCTTGCTGTC GACATCTGGT 
GCTAGTACCC CCTGCCGGGG TCGGAGGCAG CTCCCGCAGA CCCCCCTGAC CCCACGCCCC 
AGCATCACTT ACAAGACCGC CAACTCCTCG CCCGTACACT TCAGCACGTC GCCGGGCGGC 
CTCCCGCCCT TCTCCCCGGG CCGCCTGAGC CGCGGGCTGT CTGAGCACAA TGCACTGCTG 
CGTGGGGACC AGCAGCCGCC CCCGGCCGCG GTTGCCCGCA TCGGCTCCGA CCCTTACCTG 
GGCCACCGCG ACGCCGCCGA CAGCCCCATA GGCGCAGCGC CCGAGGACAC GCTCACCTTC 
GAGGAAGCCG TGGCCACCAA CTCGGGGCGT TCCTCCAGGA CTTCTTACGT CTCCTCCTTG 
ACCTCCCAGT CTCACCAAGC ACGCCGGGTG CCCAATGGCT ACCATTACAC GCTGGGGCTC 
AACACGGGGC CCGGCACTGG CACCAGGGGA CGGAGCTACT ACCACGAAGC CGACGAGGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279271.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134757.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X13, mRNA.

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CloneID	OGa145081
Clone ID Related Accession (Same CDS sequence)	XM_015279271.2
Accession Version	XM_015279271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCTTT TGTAAAGCAA 
ACTCGAGGTA CTGTATCTCG CAGCTCGTCA GTCTCCAGCG TAAATTCACC CAAGCAGCAG 
AACTCCTCCA AATCCAAGTC GGTTTGGGAG CAACGAACCA GCCAGATCCG GATGCACAAC 
TTCCGAGCCA GCTGCGAGGC TCTGTACAAC GAACTGGACC CCGAGGAGCG GGTGCGCTAC 
GCCACGACCC TACACATCCG TCCAGACATG AAGACCCACT TGGATCGGCC ACTGGTGGTA 
GAGCCAAGGG GTGAAGGGAG GAACAACATC AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG 
GTGGAGCAGA CCAAAGTCAG CTCTACCGAC GGAGCAGAAG CTCCGCGGAA GCATCACCGG 
CATCGGGATA AGGAGAAACT GGGAGAGCAA GAGAAGGGTG ATGTGACCAA AGATGAGAAT 
GGGGAATCTG GCATCAACAA TAAGGAGGAG AGGCACAGGC AGCACAGAAG CCGTAGCAAG 
GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA AGTGACCGCA GCAGGGGCCA GGAAGGAGGG 
AAGAGGCACC ATCGCCGGGG GTCGGTGGAA GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC 
CGCACGCACC GGCACTCTGC AGAACGGCAA GGCAAGGAGG GCAATGGGAC CATCAACGGG 
GCCAGGAGTG AGCGGCGGAC CCGCCACCGT GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA 
GAGCCTGGGT CCAAGGGCGA GAATGGAGAG GAGCCTCACA GACGGCACCG GTTCAGGAGC 
AGGGCGCTGT CGACGTACGA TTCGGTGGAG AAGGAGAACA GGGAGAAGGA AGGGGAGACT 
GCTGAGAAGG AGCACCAGAA TCATCAGCCC AAGGAGAATC AGTGTGAGAT TGAGGCCAGT 
GGGAGTGTGA GCATCCCGGT GCACACCCTT CCCAGCACCT ATCTGCAGAA AGTGCCAGAG 
CAGCCTGAAG ATGCTGACAA CCAGAAGAAC GTGACACGCA TGATTCAGCC GCCTCTGGAC 
AAAACCACCA CTGTGAACAT CCCCGTCACC ATCACCGCCC CGCCAGGAGA AACCACCGTC 
ATACCTATGA ACAACGTTGA GTTCGAAAGT AAAACAGAAG AGAAGAAGGA CGTTGATGAC 
TTGACGAAAA ATGGGCCTAA ACCAATCTTG CCTTACAGTT CCATGTTCAT CCTAAGTCCT 
ACGAACCCGA TCCGGCGTCT GTTCCACTAC ATCGTCAACC TGCGCTACTT CGAGATGGTC 
ATCCTCATCG TCATCGCTCT CAGCAGCATT GCCCTGGCAG CAGAAGACCC CGTCCAAGCA 
GAATCACCAA GGAACGATGC TCTGAAGTAC TTGGATTATA TATTCACGGG TGTCTTTACC 
TTCGAGATGG TGATCAAGAT GATTGACCTG GGGCTGTTAC TTCACCCTGG CTCCTATTTC 
CGTGACCTGT GGAACATCTT GGACTTCATT GTGGTCAGTG GGGCCTTGGT GGCATTTGCT 
TTCTCGAGTT TCATGGGAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTATATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGACT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGCGGGC GCATCAGTTA CACAGACATG 
TATGAGATGC TGAGACACAT GTCCCCACCT CTAGGCCTTG GAAAGAAATG CCCTGCTCGC 
GTTGCCTATA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGCC CATCCCGGAC 
ACAAGCCCCA TGAAGCGCTC CATCTCCACG CTGACCCCGC AGCGTCCTCA CCCCATGCAC 
CTCTATGAGT ACTCCCTGGA GCGCGTGCCC ACGGACCAGG TGCATCACCA CCACCACCAC 
CGCTGCCACC GGCGGAAAGA GAAGAAGCAG AAGTCCTTGG ACAGGACCAC ACATCAGCTG 
GCCGATGGGG AAGCTGTGGC CCAGTCTGGT GAATCTTCTT CCAAGGACAA GAAGCAAGAG 
CGTGGACGGT CCCAAGAGAG GAAGCAGCAC TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC 
TACTCCTGTG ATCGCTATGG CAGCCGGGAC CGATCCCAGC CCAAGTCTGC TGATCAGAGC 
AGGCCTACTT CACCCAATGG TGGGCCTGAG CAAGGTCCCC ACAGACAGGG CAGTGGTTCG 
GTTAACGGGA GCCCCTTGCT GTCGACATCT GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG 
CAGCTCCCGC AGACCCCCCT GACCCCACGC CCCAGCATCA CTTACAAGAC CGCCAACTCC 
TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG 
AGCCGCGGGC TGTCTGAGCA CAATGCACTG CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC 
GCGGTTGCCC GCATCGGCTC CGACCCTTAC CTGGGCCACC GCGACGCCGC CGACAGCCCC 
ATAGGCGCAG CGCCCGAGGA CACGCTCACC TTCGAGGAAG CCGTGGCCAC CAACTCGGGG 
CGTTCCTCCA GGACTTCTTA CGTCTCCTCC TTGACCTCCC AGTCTCACCA AGCACGCCGG 
GTGCCCAATG GCTACCATTA CACGCTGGGG CTCAACACGG GGCCCGGCAC TGGCACCAGG 
GGACGGAGCT ACTACCACGA AGCCGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134757.2
CDS	421..7425
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279271.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279271.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCTTT TGTAAAGCAA 
ACTCGAGGTA CTGTATCTCG CAGCTCGTCA GTCTCCAGCG TAAATTCACC CAAGCAGCAG 
AACTCCTCCA AATCCAAGTC GGTTTGGGAG CAACGAACCA GCCAGATCCG GATGCACAAC 
TTCCGAGCCA GCTGCGAGGC TCTGTACAAC GAACTGGACC CCGAGGAGCG GGTGCGCTAC 
GCCACGACCC TACACATCCG TCCAGACATG AAGACCCACT TGGATCGGCC ACTGGTGGTA 
GAGCCAAGGG GTGAAGGGAG GAACAACATC AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG 
GTGGAGCAGA CCAAAGTCAG CTCTACCGAC GGAGCAGAAG CTCCGCGGAA GCATCACCGG 
CATCGGGATA AGGAGAAACT GGGAGAGCAA GAGAAGGGTG ATGTGACCAA AGATGAGAAT 
GGGGAATCTG GCATCAACAA TAAGGAGGAG AGGCACAGGC AGCACAGAAG CCGTAGCAAG 
GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA AGTGACCGCA GCAGGGGCCA GGAAGGAGGG 
AAGAGGCACC ATCGCCGGGG GTCGGTGGAA GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC 
CGCACGCACC GGCACTCTGC AGAACGGCAA GGCAAGGAGG GCAATGGGAC CATCAACGGG 
GCCAGGAGTG AGCGGCGGAC CCGCCACCGT GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA 
GAGCCTGGGT CCAAGGGCGA GAATGGAGAG GAGCCTCACA GACGGCACCG GTTCAGGAGC 
AGGGCGCTGT CGACGTACGA TTCGGTGGAG AAGGAGAACA GGGAGAAGGA AGGGGAGACT 
GCTGAGAAGG AGCACCAGAA TCATCAGCCC AAGGAGAATC AGTGTGAGAT TGAGGCCAGT 
GGGAGTGTGA GCATCCCGGT GCACACCCTT CCCAGCACCT ATCTGCAGAA AGTGCCAGAG 
CAGCCTGAAG ATGCTGACAA CCAGAAGAAC GTGACACGCA TGATTCAGCC GCCTCTGGAC 
AAAACCACCA CTGTGAACAT CCCCGTCACC ATCACCGCCC CGCCAGGAGA AACCACCGTC 
ATACCTATGA ACAACGTTGA GTTCGAAAGT AAAACAGAAG AGAAGAAGGA CGTTGATGAC 
TTGACGAAAA ATGGGCCTAA ACCAATCTTG CCTTACAGTT CCATGTTCAT CCTAAGTCCT 
ACGAACCCGA TCCGGCGTCT GTTCCACTAC ATCGTCAACC TGCGCTACTT CGAGATGGTC 
ATCCTCATCG TCATCGCTCT CAGCAGCATT GCCCTGGCAG CAGAAGACCC CGTCCAAGCA 
GAATCACCAA GGAACGATGC TCTGAAGTAC TTGGATTATA TATTCACGGG TGTCTTTACC 
TTCGAGATGG TGATCAAGAT GATTGACCTG GGGCTGTTAC TTCACCCTGG CTCCTATTTC 
CGTGACCTGT GGAACATCTT GGACTTCATT GTGGTCAGTG GGGCCTTGGT GGCATTTGCT 
TTCTCGAGTT TCATGGGAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTATATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGACT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGCGGGC GCATCAGTTA CACAGACATG 
TATGAGATGC TGAGACACAT GTCCCCACCT CTAGGCCTTG GAAAGAAATG CCCTGCTCGC 
GTTGCCTATA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGCC CATCCCGGAC 
ACAAGCCCCA TGAAGCGCTC CATCTCCACG CTGACCCCGC AGCGTCCTCA CCCCATGCAC 
CTCTATGAGT ACTCCCTGGA GCGCGTGCCC ACGGACCAGG TGCATCACCA CCACCACCAC 
CGCTGCCACC GGCGGAAAGA GAAGAAGCAG AAGTCCTTGG ACAGGACCAC ACATCAGCTG 
GCCGATGGGG AAGCTGTGGC CCAGTCTGGT GAATCTTCTT CCAAGGACAA GAAGCAAGAG 
CGTGGACGGT CCCAAGAGAG GAAGCAGCAC TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC 
TACTCCTGTG ATCGCTATGG CAGCCGGGAC CGATCCCAGC CCAAGTCTGC TGATCAGAGC 
AGGCCTACTT CACCCAATGG TGGGCCTGAG CAAGGTCCCC ACAGACAGGG CAGTGGTTCG 
GTTAACGGGA GCCCCTTGCT GTCGACATCT GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG 
CAGCTCCCGC AGACCCCCCT GACCCCACGC CCCAGCATCA CTTACAAGAC CGCCAACTCC 
TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG 
AGCCGCGGGC TGTCTGAGCA CAATGCACTG CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC 
GCGGTTGCCC GCATCGGCTC CGACCCTTAC CTGGGCCACC GCGACGCCGC CGACAGCCCC 
ATAGGCGCAG CGCCCGAGGA CACGCTCACC TTCGAGGAAG CCGTGGCCAC CAACTCGGGG 
CGTTCCTCCA GGACTTCTTA CGTCTCCTCC TTGACCTCCC AGTCTCACCA AGCACGCCGG 
GTGCCCAATG GCTACCATTA CACGCTGGGG CTCAACACGG GGCCCGGCAC TGGCACCAGG 
GGACGGAGCT ACTACCACGA AGCCGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279272.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134758.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X14, mRNA.

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CloneID	OGa145082
Clone ID Related Accession (Same CDS sequence)	XM_015279272.2
Accession Version	XM_015279272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ATATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGACTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGTTGCCGG 
ATTCATTATA AGGATATGTA CAATTTGTTA CGTGTAATTG CTCCACCCCT GGGCTTAGGA 
AAGAAGTGCC CTCATAGGGT GGCATACAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGAGGA GCAAAGCACG GTCACCTGGG AGTTACCTAG CACCCATCCC GGACACAAGC 
CCCATGAAGC GCTCCATCTC CACGCTGACC CCGCAGCGTC CTCACCCCAT GCACCTCTAT 
GAGTACTCCC TGGAGCGCGT GCCCACGGAC CAGGTGCATC ACCACCACCA CCACCGCTGC 
CACCGGCGGA AAGAGAAGAA GCAGAAGTCC TTGGACAGGA CCACACATCA GCTGGCCGAT 
GGGGAAGCTG TGGCCCAGTC TGGTGAATCT TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA 
CGGTCCCAAG AGAGGAAGCA GCACTCTTCC TCCTCCTCAG AAAAGCAGCG CTTCTACTCC 
TGTGATCGCT ATGGCAGCCG GGACCGATCC CAGCCCAAGT CTGCTGATCA GAGCAGGCCT 
ACTTCACCCA ATGGTGGGCC TGAGCAAGGT CCCCACAGAC AGGGCAGTGG TTCGGTTAAC 
GGGAGCCCCT TGCTGTCGAC ATCTGGTGCT AGTACCCCCT GCCGGGGTCG GAGGCAGCTC 
CCGCAGACCC CCCTGACCCC ACGCCCCAGC ATCACTTACA AGACCGCCAA CTCCTCGCCC 
GTACACTTCA GCACGTCGCC GGGCGGCCTC CCGCCCTTCT CCCCGGGCCG CCTGAGCCGC 
GGGCTGTCTG AGCACAATGC ACTGCTGCGT GGGGACCAGC AGCCGCCCCC GGCCGCGGTT 
GCCCGCATCG GCTCCGACCC TTACCTGGGC CACCGCGACG CCGCCGACAG CCCCATAGGC 
GCAGCGCCCG AGGACACGCT CACCTTCGAG GAAGCCGTGG CCACCAACTC GGGGCGTTCC 
TCCAGGACTT CTTACGTCTC CTCCTTGACC TCCCAGTCTC ACCAAGCACG CCGGGTGCCC 
AATGGCTACC ATTACACGCT GGGGCTCAAC ACGGGGCCCG GCACTGGCAC CAGGGGACGG 
AGCTACTACC ACGAAGCCGA CGAGGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134758.2
CDS	422..7420
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279272.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279272.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ATATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGACTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGTTGCCGG 
ATTCATTATA AGGATATGTA CAATTTGTTA CGTGTAATTG CTCCACCCCT GGGCTTAGGA 
AAGAAGTGCC CTCATAGGGT GGCATACAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGAGGA GCAAAGCACG GTCACCTGGG AGTTACCTAG CACCCATCCC GGACACAAGC 
CCCATGAAGC GCTCCATCTC CACGCTGACC CCGCAGCGTC CTCACCCCAT GCACCTCTAT 
GAGTACTCCC TGGAGCGCGT GCCCACGGAC CAGGTGCATC ACCACCACCA CCACCGCTGC 
CACCGGCGGA AAGAGAAGAA GCAGAAGTCC TTGGACAGGA CCACACATCA GCTGGCCGAT 
GGGGAAGCTG TGGCCCAGTC TGGTGAATCT TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA 
CGGTCCCAAG AGAGGAAGCA GCACTCTTCC TCCTCCTCAG AAAAGCAGCG CTTCTACTCC 
TGTGATCGCT ATGGCAGCCG GGACCGATCC CAGCCCAAGT CTGCTGATCA GAGCAGGCCT 
ACTTCACCCA ATGGTGGGCC TGAGCAAGGT CCCCACAGAC AGGGCAGTGG TTCGGTTAAC 
GGGAGCCCCT TGCTGTCGAC ATCTGGTGCT AGTACCCCCT GCCGGGGTCG GAGGCAGCTC 
CCGCAGACCC CCCTGACCCC ACGCCCCAGC ATCACTTACA AGACCGCCAA CTCCTCGCCC 
GTACACTTCA GCACGTCGCC GGGCGGCCTC CCGCCCTTCT CCCCGGGCCG CCTGAGCCGC 
GGGCTGTCTG AGCACAATGC ACTGCTGCGT GGGGACCAGC AGCCGCCCCC GGCCGCGGTT 
GCCCGCATCG GCTCCGACCC TTACCTGGGC CACCGCGACG CCGCCGACAG CCCCATAGGC 
GCAGCGCCCG AGGACACGCT CACCTTCGAG GAAGCCGTGG CCACCAACTC GGGGCGTTCC 
TCCAGGACTT CTTACGTCTC CTCCTTGACC TCCCAGTCTC ACCAAGCACG CCGGGTGCCC 
AATGGCTACC ATTACACGCT GGGGCTCAAC ACGGGGCCCG GCACTGGCAC CAGGGGACGG 
AGCTACTACC ACGAAGCCGA CGAGGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279273.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134759.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X15, mRNA.

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CloneID	OGa145083
Clone ID Related Accession (Same CDS sequence)	XM_015279273.2
Accession Version	XM_015279273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCGAGTT TCATGGGAGG AACCAAAGGC 
AAGGACATCA ACACGATCAA GTCGCTGCGG GTTCTGCGGG TCCTACGACC ACTGAAGACC 
ATTAAACGGC TGCCAAAACT AAAGGCTGTC TTTGACTGCG TGGTGAATTC CCTGAAGAAC 
GTCCTCAACA TCCTCATTGT CTATATGCTC TTCATGTTCA TCTTTGCTGT CATTGCTGTG 
CAGCTCTTCA AAGGCAGATT CTTCTACTGC ACTGATGAGT CGAAAGAGCT GGAGAAGGAT 
TGCAGGGGAC AATACCTTGA CTATGAAAAA AATGAGGTGG AGGCGCAACC CAGGGAATGG 
AAAAAATATG AGTTCCATTA CGACAATGTG CTGTGGGCTC TGCTGACACT TTTCACTGTC 
TCCACAGGAG AAGGGTGGCC AACTGTGCTG AAGCACTCAG TGGATGCTAC CTACGAGGAA 
CAGGGTCCCA GCCCTGGCTA CCGAATGGAA ATGTCTATCT TTTACGTGGT GTATTTTGTT 
GTCTTCCCTT TTTTCTTTGT GAACATCTTT GTGGCGTTAA TCATAATCAC CTTCCAAGAG 
CAAGGAGATA AAGTGATGTC AGAATGCAGC CTAGAGAAAA ATGAGAGGGC CTGCATTGAC 
TTCGCCATAA GTGCCAAGCC ACTGACCCGC TACATGCCTC AGAACAAGCA GTCATTTCAG 
TACAAGATGT GGAAATTTGT GGTGTCCCCG CCCTTTGAGT ATTTTATCAT GGTCATGATT 
GCACTCAATA CTATTGTTCT CATGATGAAG TTCTATGATG CTCCAGAAGC ATATGAAGAA 
ATGTTGAAAT GCCTGAATAT TGTCTTTACA TCCATGTTTT CAATGGAGTG TGTGCTGAAG 
ATCATTGCCT TTGGTGTGCT GAATTATTTC CGAGATGCCT GGAATGTCTT TGATTTTGTA 
ACAGTGTTGG GAAGTATTAC TGATATTTTA GTAACAGAGA TTGCGGACAA CTTCATCAAC 
CTCAGCTTCC TGCGGCTCTT CAGGGCAGCA AGACTTATCA AGCTCCTTCG CCAGGGTTAC 
ACTATCCGCA TCTTGCTCTG GACGTTTGTG CAGTCATTTA AGGCCTTGCC TTATGTGTGT 
CTTCTCATTG CAATGCTCTT CTTCATCTAT GCCATTATTG GCATGCAGGT ATTTGGAAAC 
ATTGCACTAA ATGACGAAAC CTCCATCAAT CGGCACAATA ATTTCCGTAC CTTCCTTCAA 
GCCTTGATGC TTCTGTTCAG GAGTGCCACG GGGGAGGCCT GGCACGAGAT CATGCTGTCA 
TGCCTGAGCA ACAGGGCCTG CGACCCACTC TCCGGCCTCA CCAAGAACGA GTGCGGCAGC 
GACTTCGCCT ACTTCTACTT CGTGTCATTC ATCTTCCTCT GCTCCTTCCT GATGTTGAAC 
CTATTTGTGG CTGTAATCAT GGACAACTTT GAATACTTGA CAAGAGACTC CTCCATCCTT 
GGGCCCCATC ATTTGGATGA GTTTGTCCGT GTCTGGGCTG AATACGACCC AGCTGCCTGC 
GGGCGCATCA GTTACACAGA CATGTATGAG ATGCTGAGAC ACATGTCCCC ACCTCTAGGC 
CTTGGAAAGA AATGCCCTGC TCGCGTTGCC TATAAGCGCC TGGTGCGGAT GAACATGCCC 
ATCTCACCCG AGGATCTGAC AGTCCACTTC ACCTCCACGC TGATGGCCCT GATCCGCACT 
GCTCTGGAAA TCAAGCTCGC CTCAGGGGGT GTTAAGCAGC ACCAGTGTGA TGCTGAGCTG 
AGAAAGGAGA TATCTCTAGT TTGGCCCAAC CTGTCCCAGA AGACACTGGA TTTGCTGGTG 
CCTCCTCACA AACCTGATGA GATGACTGTG GGGAAGGTCT ACGCAGCGCT CATGATATTT 
GACTTCTACA AACAGAACAA GAACAGCAGG GAACAAGTCC ACCAGCCTCC TGGGGGCCTG 
TGCCAGCCCG GACCAGTGTC ACTCTTCCAC CCCTTGAAGG CCACTTTGGA GCAAACCCAG 
CCCTCGGCAT TCAGCAGTGC GAAGGCATTC CTGCGCCAGA AAAGCTCGGC CTCACTCAAC 
AATGGAGGCA CATTGCCAGC CCCAGAGGGT GGGATCAAAG AGTCCAGTTC GTGGGGGACC 
CAACGGACCC AGGACGTGTT TTATGAAACC AGGACACCAG CTTTTGAACG AGGCCACTCA 
GAGGAAATCC CCATTGAACG GGTGGTGGAA ATGAGGGAAA TCAGCCCCAC ACTTGCCAAT 
GGAGAGCACC AACCAGGGCT GGAGAGCCAG GGTCGGGCGG CTTCCATGCC GCGCCTGGCT 
GCTGAAACGC AGCCCATCCC GGACACAAGC CCCATGAAGC GCTCCATCTC CACGCTGACC 
CCGCAGCGTC CTCACCCCAT GCACCTCTAT GAGTACTCCC TGGAGCGCGT GCCCACGGAC 
CAGGTGCATC ACCACCACCA CCACCGCTGC CACCGGCGGA AAGAGAAGAA GCAGAAGTCC 
TTGGACAGGA CCACACATCA GCTGGCCGAT GGGGAAGCTG TGGCCCAGTC TGGTGAATCT 
TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA CGGTCCCAAG AGAGGAAGCA GCACTCTTCC 
TCCTCCTCAG AAAAGCAGCG CTTCTACTCC TGTGATCGCT ATGGCAGCCG GGACCGATCC 
CAGCCCAAGT CTGCTGATCA GAGCAGGCCT ACTTCACCCA ATGGTGGGCC TGAGCAAGGT 
CCCCACAGAC AGGGCAGTGG TTCGGTTAAC GGGAGCCCCT TGCTGTCGAC ATCTGGTGCT 
AGTACCCCCT GCCGGGGTCG GAGGCAGCTC CCGCAGACCC CCCTGACCCC ACGCCCCAGC 
ATCACTTACA AGACCGCCAA CTCCTCGCCC GTACACTTCA GCACGTCGCC GGGCGGCCTC 
CCGCCCTTCT CCCCGGGCCG CCTGAGCCGC GGGCTGTCTG AGCACAATGC ACTGCTGCGT 
GGGGACCAGC AGCCGCCCCC GGCCGCGGTT GCCCGCATCG GCTCCGACCC TTACCTGGGC 
CACCGCGACG CCGCCGACAG CCCCATAGGC GCAGCGCCCG AGGACACGCT CACCTTCGAG 
GAAGCCGTGG CCACCAACTC GGGGCGTTCC TCCAGGACTT CTTACGTCTC CTCCTTGACC 
TCCCAGTCTC ACCAAGCACG CCGGGTGCCC AATGGCTACC ATTACACGCT GGGGCTCAAC 
ACGGGGCCCG GCACTGGCAC CAGGGGACGG AGCTACTACC ACGAAGCCGA CGAGGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134759.2
CDS	421..7389
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279273.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279273.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCGAGTT TCATGGGAGG AACCAAAGGC 
AAGGACATCA ACACGATCAA GTCGCTGCGG GTTCTGCGGG TCCTACGACC ACTGAAGACC 
ATTAAACGGC TGCCAAAACT AAAGGCTGTC TTTGACTGCG TGGTGAATTC CCTGAAGAAC 
GTCCTCAACA TCCTCATTGT CTATATGCTC TTCATGTTCA TCTTTGCTGT CATTGCTGTG 
CAGCTCTTCA AAGGCAGATT CTTCTACTGC ACTGATGAGT CGAAAGAGCT GGAGAAGGAT 
TGCAGGGGAC AATACCTTGA CTATGAAAAA AATGAGGTGG AGGCGCAACC CAGGGAATGG 
AAAAAATATG AGTTCCATTA CGACAATGTG CTGTGGGCTC TGCTGACACT TTTCACTGTC 
TCCACAGGAG AAGGGTGGCC AACTGTGCTG AAGCACTCAG TGGATGCTAC CTACGAGGAA 
CAGGGTCCCA GCCCTGGCTA CCGAATGGAA ATGTCTATCT TTTACGTGGT GTATTTTGTT 
GTCTTCCCTT TTTTCTTTGT GAACATCTTT GTGGCGTTAA TCATAATCAC CTTCCAAGAG 
CAAGGAGATA AAGTGATGTC AGAATGCAGC CTAGAGAAAA ATGAGAGGGC CTGCATTGAC 
TTCGCCATAA GTGCCAAGCC ACTGACCCGC TACATGCCTC AGAACAAGCA GTCATTTCAG 
TACAAGATGT GGAAATTTGT GGTGTCCCCG CCCTTTGAGT ATTTTATCAT GGTCATGATT 
GCACTCAATA CTATTGTTCT CATGATGAAG TTCTATGATG CTCCAGAAGC ATATGAAGAA 
ATGTTGAAAT GCCTGAATAT TGTCTTTACA TCCATGTTTT CAATGGAGTG TGTGCTGAAG 
ATCATTGCCT TTGGTGTGCT GAATTATTTC CGAGATGCCT GGAATGTCTT TGATTTTGTA 
ACAGTGTTGG GAAGTATTAC TGATATTTTA GTAACAGAGA TTGCGGACAA CTTCATCAAC 
CTCAGCTTCC TGCGGCTCTT CAGGGCAGCA AGACTTATCA AGCTCCTTCG CCAGGGTTAC 
ACTATCCGCA TCTTGCTCTG GACGTTTGTG CAGTCATTTA AGGCCTTGCC TTATGTGTGT 
CTTCTCATTG CAATGCTCTT CTTCATCTAT GCCATTATTG GCATGCAGGT ATTTGGAAAC 
ATTGCACTAA ATGACGAAAC CTCCATCAAT CGGCACAATA ATTTCCGTAC CTTCCTTCAA 
GCCTTGATGC TTCTGTTCAG GAGTGCCACG GGGGAGGCCT GGCACGAGAT CATGCTGTCA 
TGCCTGAGCA ACAGGGCCTG CGACCCACTC TCCGGCCTCA CCAAGAACGA GTGCGGCAGC 
GACTTCGCCT ACTTCTACTT CGTGTCATTC ATCTTCCTCT GCTCCTTCCT GATGTTGAAC 
CTATTTGTGG CTGTAATCAT GGACAACTTT GAATACTTGA CAAGAGACTC CTCCATCCTT 
GGGCCCCATC ATTTGGATGA GTTTGTCCGT GTCTGGGCTG AATACGACCC AGCTGCCTGC 
GGGCGCATCA GTTACACAGA CATGTATGAG ATGCTGAGAC ACATGTCCCC ACCTCTAGGC 
CTTGGAAAGA AATGCCCTGC TCGCGTTGCC TATAAGCGCC TGGTGCGGAT GAACATGCCC 
ATCTCACCCG AGGATCTGAC AGTCCACTTC ACCTCCACGC TGATGGCCCT GATCCGCACT 
GCTCTGGAAA TCAAGCTCGC CTCAGGGGGT GTTAAGCAGC ACCAGTGTGA TGCTGAGCTG 
AGAAAGGAGA TATCTCTAGT TTGGCCCAAC CTGTCCCAGA AGACACTGGA TTTGCTGGTG 
CCTCCTCACA AACCTGATGA GATGACTGTG GGGAAGGTCT ACGCAGCGCT CATGATATTT 
GACTTCTACA AACAGAACAA GAACAGCAGG GAACAAGTCC ACCAGCCTCC TGGGGGCCTG 
TGCCAGCCCG GACCAGTGTC ACTCTTCCAC CCCTTGAAGG CCACTTTGGA GCAAACCCAG 
CCCTCGGCAT TCAGCAGTGC GAAGGCATTC CTGCGCCAGA AAAGCTCGGC CTCACTCAAC 
AATGGAGGCA CATTGCCAGC CCCAGAGGGT GGGATCAAAG AGTCCAGTTC GTGGGGGACC 
CAACGGACCC AGGACGTGTT TTATGAAACC AGGACACCAG CTTTTGAACG AGGCCACTCA 
GAGGAAATCC CCATTGAACG GGTGGTGGAA ATGAGGGAAA TCAGCCCCAC ACTTGCCAAT 
GGAGAGCACC AACCAGGGCT GGAGAGCCAG GGTCGGGCGG CTTCCATGCC GCGCCTGGCT 
GCTGAAACGC AGCCCATCCC GGACACAAGC CCCATGAAGC GCTCCATCTC CACGCTGACC 
CCGCAGCGTC CTCACCCCAT GCACCTCTAT GAGTACTCCC TGGAGCGCGT GCCCACGGAC 
CAGGTGCATC ACCACCACCA CCACCGCTGC CACCGGCGGA AAGAGAAGAA GCAGAAGTCC 
TTGGACAGGA CCACACATCA GCTGGCCGAT GGGGAAGCTG TGGCCCAGTC TGGTGAATCT 
TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA CGGTCCCAAG AGAGGAAGCA GCACTCTTCC 
TCCTCCTCAG AAAAGCAGCG CTTCTACTCC TGTGATCGCT ATGGCAGCCG GGACCGATCC 
CAGCCCAAGT CTGCTGATCA GAGCAGGCCT ACTTCACCCA ATGGTGGGCC TGAGCAAGGT 
CCCCACAGAC AGGGCAGTGG TTCGGTTAAC GGGAGCCCCT TGCTGTCGAC ATCTGGTGCT 
AGTACCCCCT GCCGGGGTCG GAGGCAGCTC CCGCAGACCC CCCTGACCCC ACGCCCCAGC 
ATCACTTACA AGACCGCCAA CTCCTCGCCC GTACACTTCA GCACGTCGCC GGGCGGCCTC 
CCGCCCTTCT CCCCGGGCCG CCTGAGCCGC GGGCTGTCTG AGCACAATGC ACTGCTGCGT 
GGGGACCAGC AGCCGCCCCC GGCCGCGGTT GCCCGCATCG GCTCCGACCC TTACCTGGGC 
CACCGCGACG CCGCCGACAG CCCCATAGGC GCAGCGCCCG AGGACACGCT CACCTTCGAG 
GAAGCCGTGG CCACCAACTC GGGGCGTTCC TCCAGGACTT CTTACGTCTC CTCCTTGACC 
TCCCAGTCTC ACCAAGCACG CCGGGTGCCC AATGGCTACC ATTACACGCT GGGGCTCAAC 
ACGGGGCCCG GCACTGGCAC CAGGGGACGG AGCTACTACC ACGAAGCCGA CGAGGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279274.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134760.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X16, mRNA.

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CloneID	OGa145084
Clone ID Related Accession (Same CDS sequence)	XM_015279274.2
Accession Version	XM_015279274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCCAA GCAGCAGAAC 
TCCTCCAAAT CCAAGTCGGT TTGGGAGCAA CGAACCAGCC AGATCCGGAT GCACAACTTC 
CGAGCCAGCT GCGAGGCTCT GTACAACGAA CTGGACCCCG AGGAGCGGGT GCGCTACGCC 
ACGACCCTAC ACATCCGTCC AGACATGAAG ACCCACTTGG ATCGGCCACT GGTGGTAGAG 
CCAAGGGGTG AAGGGAGGAA CAACATCAGT AAGCTGAGCC CTGTGGATGT GCAGGAGGTG 
GAGCAGACCA AAGTCAGCTC TACCGACGGA GCAGAAGCTC CGCGGAAGCA TCACCGGCAT 
CGGGATAAGG AGAAACTGGG AGAGCAAGAG AAGGGTGATG TGACCAAAGA TGAGAATGGG 
GAATCTGGCA TCAACAATAA GGAGGAGAGG CACAGGCAGC ACAGAAGCCG TAGCAAGGAG 
GTGGAAGGGG GCAGCAAGGA AGGGAAAAGT GACCGCAGCA GGGGCCAGGA AGGAGGGAAG 
AGGCACCATC GCCGGGGGTC GGTGGAAGAA GGCGTTGAGA AGGAGCACCG GAGGCACCGC 
ACGCACCGGC ACTCTGCAGA ACGGCAAGGC AAGGAGGGCA ATGGGACCAT CAACGGGGCC 
AGGAGTGAGC GGCGGACCCG CCACCGTGGA GGGTCGAGGT CTGGGAACCG GGAAGGAGAG 
CCTGGGTCCA AGGGCGAGAA TGGAGAGGAG CCTCACAGAC GGCACCGGTT CAGGAGCAGG 
GCGCTGTCGA CGTACGATTC GGTGGAGAAG GAGAACAGGG AGAAGGAAGG GGAGACTGCT 
GAGAAGGAGC ACCAGAATCA TCAGCCCAAG GAGAATCAGT GTGAGATTGA GGCCAGTGGG 
AGTGTGAGCA TCCCGGTGCA CACCCTTCCC AGCACCTATC TGCAGAAAGT GCCAGAGCAG 
CCTGAAGATG CTGACAACCA GAAGAACGTG ACACGCATGA TTCAGCCGCC TCTGGACAAA 
ACCACCACTG TGAACATCCC CGTCACCATC ACCGCCCCGC CAGGAGAAAC CACCGTCATA 
CCTATGAACA ACGTTGAGTT CGAAAGTAAA ACAGAAGAGA AGAAGGACGT TGATGACTTG 
ACGAAAAATG GGCCTAAACC AATCTTGCCT TACAGTTCCA TGTTCATCCT AAGTCCTACG 
AACCCGATCC GGCGTCTGTT CCACTACATC GTCAACCTGC GCTACTTCGA GATGGTCATC 
CTCATCGTCA TCGCTCTCAG CAGCATTGCC CTGGCAGCAG AAGACCCCGT CCAAGCAGAA 
TCACCAAGGA ACGATGCTCT GAAGTACTTG GATTATATAT TCACGGGTGT CTTTACCTTC 
GAGATGGTGA TCAAGATGAT TGACCTGGGG CTGTTACTTC ACCCTGGCTC CTATTTCCGT 
GACCTGTGGA ACATCTTGGA CTTCATTGTG GTCAGTGGGG CCTTGGTGGC ATTTGCTTTC 
TCAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTATA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACACGGACA ACTTCATCAA CCTCAGCTTC CTGCGGCTCT TCAGGGCAGC AAGACTTATC 
AAGCTCCTTC GCCAGGGTTA CACTATCCGC ATCTTGCTCT GGACGTTTGT GCAGTCATTT 
AAGGCCTTGC CTTATGTGTG TCTTCTCATT GCAATGCTCT TCTTCATCTA TGCCATTATT 
GGCATGCAGG TATTTGGAAA CATTGCACTA AATGACGAAA CCTCCATCAA TCGGCACAAT 
AATTTCCGTA CCTTCCTTCA AGCCTTGATG CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC ATGCCTGAGC AACAGGGCCT GCGACCCACT CTCCGGCCTC 
ACCAAGAACG AGTGCGGCAG CGACTTCGCC TACTTCTACT TCGTGTCATT CATCTTCCTC 
TGCTCCTTCC TGATGTTGAA CCTATTTGTG GCTGTAATCA TGGACAACTT TGAATACTTG 
ACAAGAGACT CCTCCATCCT TGGGCCCCAT CATTTGGATG AGTTTGTCCG TGTCTGGGCT 
GAATACGACC CAGCTGCCTG TTGCCGGATT CATTATAAGG ATATGTACAA TTTGTTACGT 
GTAATTGCTC CACCCCTGGG CTTAGGAAAG AAGTGCCCTC ATAGGGTGGC ATACAAGCGC 
CTGGTGCGGA TGAACATGCC CATCTCACCC GAGGATCTGA CAGTCCACTT CACCTCCACG 
CTGATGGCCC TGATCCGCAC TGCTCTGGAA ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAGGAG ATATCTCTAG TTTGGCCCAA CCTGTCCCAG 
AAGACACTGG ATTTGCTGGT GCCTCCTCAC AAACCTGATG AGATGACTGT GGGGAAGGTC 
TACGCAGCGC TCATGATATT TGACTTCTAC AAACAGAACA AGAACAGCAG GGAACAAGTC 
CACCAGCCTC CTGGGGGCCT GTGCCAGCCC GGACCAGTGT CACTCTTCCA CCCCTTGAAG 
GCCACTTTGG AGCAAACCCA GCCCTCGGCA TTCAGCAGTG CGAAGGCATT CCTGCGCCAG 
AAAAGCTCGG CCTCACTCAA CAATGGAGGC ACATTGCCAG CCCCAGAGGG TGGGATCAAA 
GAGTCCAGTT CGTGGGGGAC CCAACGGACC CAGGACGTGT TTTATGAAAC CAGGACACCA 
GCTTTTGAAC GAGGCCACTC AGAGGAAATC CCCATTGAAC GGGTGGTGGA AATGAGGGAA 
ATCAGCCCCA CACTTGCCAA TGGAGAGCAC CAACCAGGGC TGGAGAGCCA GGGTCGGGCG 
GCTTCCATGC CGCGCCTGGC TGCTGAAACG CAGAGGAGCA AAGCACGGTC ACCTGGGAGT 
TACCTAGCAC CCATCCCGGA CACAAGCCCC ATGAAGCGCT CCATCTCCAC GCTGACCCCG 
CAGCGTCCTC ACCCCATGCA CCTCTATGAG TACTCCCTGG AGCGCGTGCC CACGGACCAG 
GTGCATCACC ACCACCACCA CCGCTGCCAC CGGCGGAAAG AGAAGAAGCA GAAGTCCTTG 
GACAGGACCA CACATCAGCT GGCCGATGGG GAAGCTGTGG CCCAGTCTGG TGAATCTTCT 
TCCAAGGACA AGAAGCAAGA GCGTGGACGG TCCCAAGAGA GGAAGCAGCA CTCTTCCTCC 
TCCTCAGAAA AGCAGCGCTT CTACTCCTGT GATCGCTATG GCAGCCGGGA CCGATCCCAG 
CCCAAGTCTG CTGATCAGAG CAGGCCTACT TCACCCAATG GTGGGCCTGA GCAAGGTCCC 
CACAGACAGG GCAGTGGTTC GGTTAACGGG AGCCCCTTGC TGTCGACATC TGGTGCTAGT 
ACCCCCTGCC GGGGTCGGAG GCAGCTCCCG CAGACCCCCC TGACCCCACG CCCCAGCATC 
ACTTACAAGA CCGCCAACTC CTCGCCCGTA CACTTCAGCA CGTCGCCGGG CGGCCTCCCG 
CCCTTCTCCC CGGGCCGCCT GAGCCGCGGG CTGTCTGAGC ACAATGCACT GCTGCGTGGG 
GACCAGCAGC CGCCCCCGGC CGCGGTTGCC CGCATCGGCT CCGACCCTTA CCTGGGCCAC 
CGCGACGCCG CCGACAGCCC CATAGGCGCA GCGCCCGAGG ACACGCTCAC CTTCGAGGAA 
GCCGTGGCCA CCAACTCGGG GCGTTCCTCC AGGACTTCTT ACGTCTCCTC CTTGACCTCC 
CAGTCTCACC AAGCACGCCG GGTGCCCAAT GGCTACCATT ACACGCTGGG GCTCAACACG 
GGGCCCGGCA CTGGCACCAG GGGACGGAGC TACTACCACG AAGCCGACGA GGATGACTGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134760.2
CDS	422..7387
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279274.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279274.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCCAA GCAGCAGAAC 
TCCTCCAAAT CCAAGTCGGT TTGGGAGCAA CGAACCAGCC AGATCCGGAT GCACAACTTC 
CGAGCCAGCT GCGAGGCTCT GTACAACGAA CTGGACCCCG AGGAGCGGGT GCGCTACGCC 
ACGACCCTAC ACATCCGTCC AGACATGAAG ACCCACTTGG ATCGGCCACT GGTGGTAGAG 
CCAAGGGGTG AAGGGAGGAA CAACATCAGT AAGCTGAGCC CTGTGGATGT GCAGGAGGTG 
GAGCAGACCA AAGTCAGCTC TACCGACGGA GCAGAAGCTC CGCGGAAGCA TCACCGGCAT 
CGGGATAAGG AGAAACTGGG AGAGCAAGAG AAGGGTGATG TGACCAAAGA TGAGAATGGG 
GAATCTGGCA TCAACAATAA GGAGGAGAGG CACAGGCAGC ACAGAAGCCG TAGCAAGGAG 
GTGGAAGGGG GCAGCAAGGA AGGGAAAAGT GACCGCAGCA GGGGCCAGGA AGGAGGGAAG 
AGGCACCATC GCCGGGGGTC GGTGGAAGAA GGCGTTGAGA AGGAGCACCG GAGGCACCGC 
ACGCACCGGC ACTCTGCAGA ACGGCAAGGC AAGGAGGGCA ATGGGACCAT CAACGGGGCC 
AGGAGTGAGC GGCGGACCCG CCACCGTGGA GGGTCGAGGT CTGGGAACCG GGAAGGAGAG 
CCTGGGTCCA AGGGCGAGAA TGGAGAGGAG CCTCACAGAC GGCACCGGTT CAGGAGCAGG 
GCGCTGTCGA CGTACGATTC GGTGGAGAAG GAGAACAGGG AGAAGGAAGG GGAGACTGCT 
GAGAAGGAGC ACCAGAATCA TCAGCCCAAG GAGAATCAGT GTGAGATTGA GGCCAGTGGG 
AGTGTGAGCA TCCCGGTGCA CACCCTTCCC AGCACCTATC TGCAGAAAGT GCCAGAGCAG 
CCTGAAGATG CTGACAACCA GAAGAACGTG ACACGCATGA TTCAGCCGCC TCTGGACAAA 
ACCACCACTG TGAACATCCC CGTCACCATC ACCGCCCCGC CAGGAGAAAC CACCGTCATA 
CCTATGAACA ACGTTGAGTT CGAAAGTAAA ACAGAAGAGA AGAAGGACGT TGATGACTTG 
ACGAAAAATG GGCCTAAACC AATCTTGCCT TACAGTTCCA TGTTCATCCT AAGTCCTACG 
AACCCGATCC GGCGTCTGTT CCACTACATC GTCAACCTGC GCTACTTCGA GATGGTCATC 
CTCATCGTCA TCGCTCTCAG CAGCATTGCC CTGGCAGCAG AAGACCCCGT CCAAGCAGAA 
TCACCAAGGA ACGATGCTCT GAAGTACTTG GATTATATAT TCACGGGTGT CTTTACCTTC 
GAGATGGTGA TCAAGATGAT TGACCTGGGG CTGTTACTTC ACCCTGGCTC CTATTTCCGT 
GACCTGTGGA ACATCTTGGA CTTCATTGTG GTCAGTGGGG CCTTGGTGGC ATTTGCTTTC 
TCAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTATA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACACGGACA ACTTCATCAA CCTCAGCTTC CTGCGGCTCT TCAGGGCAGC AAGACTTATC 
AAGCTCCTTC GCCAGGGTTA CACTATCCGC ATCTTGCTCT GGACGTTTGT GCAGTCATTT 
AAGGCCTTGC CTTATGTGTG TCTTCTCATT GCAATGCTCT TCTTCATCTA TGCCATTATT 
GGCATGCAGG TATTTGGAAA CATTGCACTA AATGACGAAA CCTCCATCAA TCGGCACAAT 
AATTTCCGTA CCTTCCTTCA AGCCTTGATG CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC ATGCCTGAGC AACAGGGCCT GCGACCCACT CTCCGGCCTC 
ACCAAGAACG AGTGCGGCAG CGACTTCGCC TACTTCTACT TCGTGTCATT CATCTTCCTC 
TGCTCCTTCC TGATGTTGAA CCTATTTGTG GCTGTAATCA TGGACAACTT TGAATACTTG 
ACAAGAGACT CCTCCATCCT TGGGCCCCAT CATTTGGATG AGTTTGTCCG TGTCTGGGCT 
GAATACGACC CAGCTGCCTG TTGCCGGATT CATTATAAGG ATATGTACAA TTTGTTACGT 
GTAATTGCTC CACCCCTGGG CTTAGGAAAG AAGTGCCCTC ATAGGGTGGC ATACAAGCGC 
CTGGTGCGGA TGAACATGCC CATCTCACCC GAGGATCTGA CAGTCCACTT CACCTCCACG 
CTGATGGCCC TGATCCGCAC TGCTCTGGAA ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAGGAG ATATCTCTAG TTTGGCCCAA CCTGTCCCAG 
AAGACACTGG ATTTGCTGGT GCCTCCTCAC AAACCTGATG AGATGACTGT GGGGAAGGTC 
TACGCAGCGC TCATGATATT TGACTTCTAC AAACAGAACA AGAACAGCAG GGAACAAGTC 
CACCAGCCTC CTGGGGGCCT GTGCCAGCCC GGACCAGTGT CACTCTTCCA CCCCTTGAAG 
GCCACTTTGG AGCAAACCCA GCCCTCGGCA TTCAGCAGTG CGAAGGCATT CCTGCGCCAG 
AAAAGCTCGG CCTCACTCAA CAATGGAGGC ACATTGCCAG CCCCAGAGGG TGGGATCAAA 
GAGTCCAGTT CGTGGGGGAC CCAACGGACC CAGGACGTGT TTTATGAAAC CAGGACACCA 
GCTTTTGAAC GAGGCCACTC AGAGGAAATC CCCATTGAAC GGGTGGTGGA AATGAGGGAA 
ATCAGCCCCA CACTTGCCAA TGGAGAGCAC CAACCAGGGC TGGAGAGCCA GGGTCGGGCG 
GCTTCCATGC CGCGCCTGGC TGCTGAAACG CAGAGGAGCA AAGCACGGTC ACCTGGGAGT 
TACCTAGCAC CCATCCCGGA CACAAGCCCC ATGAAGCGCT CCATCTCCAC GCTGACCCCG 
CAGCGTCCTC ACCCCATGCA CCTCTATGAG TACTCCCTGG AGCGCGTGCC CACGGACCAG 
GTGCATCACC ACCACCACCA CCGCTGCCAC CGGCGGAAAG AGAAGAAGCA GAAGTCCTTG 
GACAGGACCA CACATCAGCT GGCCGATGGG GAAGCTGTGG CCCAGTCTGG TGAATCTTCT 
TCCAAGGACA AGAAGCAAGA GCGTGGACGG TCCCAAGAGA GGAAGCAGCA CTCTTCCTCC 
TCCTCAGAAA AGCAGCGCTT CTACTCCTGT GATCGCTATG GCAGCCGGGA CCGATCCCAG 
CCCAAGTCTG CTGATCAGAG CAGGCCTACT TCACCCAATG GTGGGCCTGA GCAAGGTCCC 
CACAGACAGG GCAGTGGTTC GGTTAACGGG AGCCCCTTGC TGTCGACATC TGGTGCTAGT 
ACCCCCTGCC GGGGTCGGAG GCAGCTCCCG CAGACCCCCC TGACCCCACG CCCCAGCATC 
ACTTACAAGA CCGCCAACTC CTCGCCCGTA CACTTCAGCA CGTCGCCGGG CGGCCTCCCG 
CCCTTCTCCC CGGGCCGCCT GAGCCGCGGG CTGTCTGAGC ACAATGCACT GCTGCGTGGG 
GACCAGCAGC CGCCCCCGGC CGCGGTTGCC CGCATCGGCT CCGACCCTTA CCTGGGCCAC 
CGCGACGCCG CCGACAGCCC CATAGGCGCA GCGCCCGAGG ACACGCTCAC CTTCGAGGAA 
GCCGTGGCCA CCAACTCGGG GCGTTCCTCC AGGACTTCTT ACGTCTCCTC CTTGACCTCC 
CAGTCTCACC AAGCACGCCG GGTGCCCAAT GGCTACCATT ACACGCTGGG GCTCAACACG 
GGGCCCGGCA CTGGCACCAG GGGACGGAGC TACTACCACG AAGCCGACGA GGATGACTGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_025156020.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025011788.1 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X17, mRNA.

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CloneID	OGa145085
Clone ID Related Accession (Same CDS sequence)	XM_025156020.1
Accession Version	XM_025156020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ATATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACAACT TCATCAACCT CAGCTTCCTG 
CGGCTCTTCA GGGCAGCAAG ACTTATCAAG CTCCTTCGCC AGGGTTACAC TATCCGCATC 
TTGCTCTGGA CGTTTGTGCA GTCATTTAAG GCCTTGCCTT ATGTGTGTCT TCTCATTGCA 
ATGCTCTTCT TCATCTATGC CATTATTGGC ATGCAGGTAT TTGGAAACAT TGCACTAAAT 
GACGAAACCT CCATCAATCG GCACAATAAT TTCCGTACCT TCCTTCAAGC CTTGATGCTT 
CTGTTCAGGA GTGCCACGGG GGAGGCCTGG CACGAGATCA TGCTGTCATG CCTGAGCAAC 
AGGGCCTGCG ACCCACTCTC CGGCCTCACC AAGAACGAGT GCGGCAGCGA CTTCGCCTAC 
TTCTACTTCG TGTCATTCAT CTTCCTCTGC TCCTTCCTGA TGTTGAACCT ATTTGTGGCT 
GTAATCATGG ACAACTTTGA ATACTTGACA AGAGACTCCT CCATCCTTGG GCCCCATCAT 
TTGGATGAGT TTGTCCGTGT CTGGGCTGAA TACGACCCAG CTGCCTGCGG GCGCATCAGT 
TACACAGACA TGTATGAGAT GCTGAGACAC ATGTCCCCAC CTCTAGGCCT TGGAAAGAAA 
TGCCCTGCTC GCGTTGCCTA TAAGCGCCTG GTGCGGATGA ACATGCCCAT CTCACCCGAG 
GATCTGACAG TCCACTTCAC CTCCACGCTG ATGGCCCTGA TCCGCACTGC TCTGGAAATC 
AAGCTCGCCT CAGGGGGTGT TAAGCAGCAC CAGTGTGATG CTGAGCTGAG AAAGGAGATA 
TCTCTAGTTT GGCCCAACCT GTCCCAGAAG ACACTGGATT TGCTGGTGCC TCCTCACAAA 
CCTGATGAGA TGACTGTGGG GAAGGTCTAC GCAGCGCTCA TGATATTTGA CTTCTACAAA 
CAGAACAAGA ACAGCAGGGA ACAAGTCCAC CAGCCTCCTG GGGGCCTGTG CCAGCCCGGA 
CCAGTGTCAC TCTTCCACCC CTTGAAGGCC ACTTTGGAGC AAACCCAGCC CTCGGCATTC 
AGCAGTGCGA AGGCATTCCT GCGCCAGAAA AGCTCGGCCT CACTCAACAA TGGAGGCACA 
TTGCCAGCCC CAGAGGGTGG GATCAAAGAG TCCAGTTCGT GGGGGACCCA ACGGACCCAG 
GACGTGTTTT ATGAAACCAG GACACCAGCT TTTGAACGAG GCCACTCAGA GGAAATCCCC 
ATTGAACGGG TGGTGGAAAT GAGGGAAATC AGCCCCACAC TTGCCAATGG AGAGCACCAA 
CCAGGGCTGG AGAGCCAGGG TCGGGCGGCT TCCATGCCGC GCCTGGCTGC TGAAACGCAG 
CCCATCCCGG ACACAAGCCC CATGAAGCGC TCCATCTCCA CGCTGACCCC GCAGCGTCCT 
CACCCCATGC ACCTCTATGA GTACTCCCTG GAGCGCGTGC CCACGGACCA GGTGCATCAC 
CACCACCACC ACCGCTGCCA CCGGCGGAAA GAGAAGAAGC AGAAGTCCTT GGACAGGACC 
ACACATCAGC TGGCCGATGG GGAAGCTGTG GCCCAGTCTG GTGAATCTTC TTCCAAGGAC 
AAGAAGCAAG AGCGTGGACG GTCCCAAGAG AGGAAGCAGC ACTCTTCCTC CTCCTCAGAA 
AAGCAGCGCT TCTACTCCTG TGATCGCTAT GGCAGCCGGG ACCGATCCCA GCCCAAGTCT 
GCTGATCAGA GCAGGCCTAC TTCACCCAAT GGTGGGCCTG AGCAAGGTCC CCACAGACAG 
GGCAGTGGTT CGGTTAACGG GAGCCCCTTG CTGTCGACAT CTGGTGCTAG TACCCCCTGC 
CGGGGTCGGA GGCAGCTCCC GCAGACCCCC CTGACCCCAC GCCCCAGCAT CACTTACAAG 
ACCGCCAACT CCTCGCCCGT ACACTTCAGC ACGTCGCCGG GCGGCCTCCC GCCCTTCTCC 
CCGGGCCGCC TGAGCCGCGG GCTGTCTGAG CACAATGCAC TGCTGCGTGG GGACCAGCAG 
CCGCCCCCGG CCGCGGTTGC CCGCATCGGC TCCGACCCTT ACCTGGGCCA CCGCGACGCC 
GCCGACAGCC CCATAGGCGC AGCGCCCGAG GACACGCTCA CCTTCGAGGA AGCCGTGGCC 
ACCAACTCGG GGCGTTCCTC CAGGACTTCT TACGTCTCCT CCTTGACCTC CCAGTCTCAC 
CAAGCACGCC GGGTGCCCAA TGGCTACCAT TACACGCTGG GGCTCAACAC GGGGCCCGGC 
ACTGGCACCA GGGGACGGAG CTACTACCAC GAAGCCGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025011788.1
CDS	421..7377
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_025156020.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025156020.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ATATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACAACT TCATCAACCT CAGCTTCCTG 
CGGCTCTTCA GGGCAGCAAG ACTTATCAAG CTCCTTCGCC AGGGTTACAC TATCCGCATC 
TTGCTCTGGA CGTTTGTGCA GTCATTTAAG GCCTTGCCTT ATGTGTGTCT TCTCATTGCA 
ATGCTCTTCT TCATCTATGC CATTATTGGC ATGCAGGTAT TTGGAAACAT TGCACTAAAT 
GACGAAACCT CCATCAATCG GCACAATAAT TTCCGTACCT TCCTTCAAGC CTTGATGCTT 
CTGTTCAGGA GTGCCACGGG GGAGGCCTGG CACGAGATCA TGCTGTCATG CCTGAGCAAC 
AGGGCCTGCG ACCCACTCTC CGGCCTCACC AAGAACGAGT GCGGCAGCGA CTTCGCCTAC 
TTCTACTTCG TGTCATTCAT CTTCCTCTGC TCCTTCCTGA TGTTGAACCT ATTTGTGGCT 
GTAATCATGG ACAACTTTGA ATACTTGACA AGAGACTCCT CCATCCTTGG GCCCCATCAT 
TTGGATGAGT TTGTCCGTGT CTGGGCTGAA TACGACCCAG CTGCCTGCGG GCGCATCAGT 
TACACAGACA TGTATGAGAT GCTGAGACAC ATGTCCCCAC CTCTAGGCCT TGGAAAGAAA 
TGCCCTGCTC GCGTTGCCTA TAAGCGCCTG GTGCGGATGA ACATGCCCAT CTCACCCGAG 
GATCTGACAG TCCACTTCAC CTCCACGCTG ATGGCCCTGA TCCGCACTGC TCTGGAAATC 
AAGCTCGCCT CAGGGGGTGT TAAGCAGCAC CAGTGTGATG CTGAGCTGAG AAAGGAGATA 
TCTCTAGTTT GGCCCAACCT GTCCCAGAAG ACACTGGATT TGCTGGTGCC TCCTCACAAA 
CCTGATGAGA TGACTGTGGG GAAGGTCTAC GCAGCGCTCA TGATATTTGA CTTCTACAAA 
CAGAACAAGA ACAGCAGGGA ACAAGTCCAC CAGCCTCCTG GGGGCCTGTG CCAGCCCGGA 
CCAGTGTCAC TCTTCCACCC CTTGAAGGCC ACTTTGGAGC AAACCCAGCC CTCGGCATTC 
AGCAGTGCGA AGGCATTCCT GCGCCAGAAA AGCTCGGCCT CACTCAACAA TGGAGGCACA 
TTGCCAGCCC CAGAGGGTGG GATCAAAGAG TCCAGTTCGT GGGGGACCCA ACGGACCCAG 
GACGTGTTTT ATGAAACCAG GACACCAGCT TTTGAACGAG GCCACTCAGA GGAAATCCCC 
ATTGAACGGG TGGTGGAAAT GAGGGAAATC AGCCCCACAC TTGCCAATGG AGAGCACCAA 
CCAGGGCTGG AGAGCCAGGG TCGGGCGGCT TCCATGCCGC GCCTGGCTGC TGAAACGCAG 
CCCATCCCGG ACACAAGCCC CATGAAGCGC TCCATCTCCA CGCTGACCCC GCAGCGTCCT 
CACCCCATGC ACCTCTATGA GTACTCCCTG GAGCGCGTGC CCACGGACCA GGTGCATCAC 
CACCACCACC ACCGCTGCCA CCGGCGGAAA GAGAAGAAGC AGAAGTCCTT GGACAGGACC 
ACACATCAGC TGGCCGATGG GGAAGCTGTG GCCCAGTCTG GTGAATCTTC TTCCAAGGAC 
AAGAAGCAAG AGCGTGGACG GTCCCAAGAG AGGAAGCAGC ACTCTTCCTC CTCCTCAGAA 
AAGCAGCGCT TCTACTCCTG TGATCGCTAT GGCAGCCGGG ACCGATCCCA GCCCAAGTCT 
GCTGATCAGA GCAGGCCTAC TTCACCCAAT GGTGGGCCTG AGCAAGGTCC CCACAGACAG 
GGCAGTGGTT CGGTTAACGG GAGCCCCTTG CTGTCGACAT CTGGTGCTAG TACCCCCTGC 
CGGGGTCGGA GGCAGCTCCC GCAGACCCCC CTGACCCCAC GCCCCAGCAT CACTTACAAG 
ACCGCCAACT CCTCGCCCGT ACACTTCAGC ACGTCGCCGG GCGGCCTCCC GCCCTTCTCC 
CCGGGCCGCC TGAGCCGCGG GCTGTCTGAG CACAATGCAC TGCTGCGTGG GGACCAGCAG 
CCGCCCCCGG CCGCGGTTGC CCGCATCGGC TCCGACCCTT ACCTGGGCCA CCGCGACGCC 
GCCGACAGCC CCATAGGCGC AGCGCCCGAG GACACGCTCA CCTTCGAGGA AGCCGTGGCC 
ACCAACTCGG GGCGTTCCTC CAGGACTTCT TACGTCTCCT CCTTGACCTC CCAGTCTCAC 
CAAGCACGCC GGGTGCCCAA TGGCTACCAT TACACGCTGG GGCTCAACAC GGGGCCCGGC 
ACTGGCACCA GGGGACGGAG CTACTACCAC GAAGCCGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279275.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134761.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X18, mRNA.

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CloneID	OGa145086
Clone ID Related Accession (Same CDS sequence)	XM_015279275.2
Accession Version	XM_015279275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ATATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGACTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGCGGGCGC ATCAGTTACA CAGACATGTA TGAGATGCTG 
AGACACATGT CCCCACCTCT AGGCCTTGGA AAGAAATGCC CTGCTCGCGT TGCCTATAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGTGGT TCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134761.2
CDS	422..7009
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279275.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279275.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ATATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGACTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGCGGGCGC ATCAGTTACA CAGACATGTA TGAGATGCTG 
AGACACATGT CCCCACCTCT AGGCCTTGGA AAGAAATGCC CTGCTCGCGT TGCCTATAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGTGGT TCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279276.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134762.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X19, mRNA.

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CloneID	OGa145087
Clone ID Related Accession (Same CDS sequence)	XM_015279276.2
Accession Version	XM_015279276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTATATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG ACTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGTT GCCGGATTCA TTATAAGGAT ATGTACAATT TGTTACGTGT AATTGCTCCA 
CCCCTGGGCT TAGGAAAGAA GTGCCCTCAT AGGGTGGCAT ACAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACA GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACACTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AACAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ATGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCGGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GAGGAGCAAA GCACGGTCAC CTGGGAGTTA CCTAGCACCC 
ATCCCGGACA CAAGCCCCAT GAAGCGCTCC ATCTCCACGC TGACCCCGCA GCGTCCTCAC 
CCCATGCACC TCTATGAGTA CTCCCTGGAG CGCGTGCCCA CGGACCAGGT GCATCACCAC 
CACCACCACC GCTGCCACCG GCGGAAAGAG AAGAAGCAGA AGTCCTTGGA CAGGACCACA 
CATCAGCTGG CCGATGGGGA AGCTGTGGCC CAGTCTGGTG AATCTTCTTC CAAGGACAAG 
AAGCAAGAGC GTGGACGGTC CCAAGAGAGG AAGCAGCACT CTTCCTCCTC CTCAGAAAAG 
CAGCGCTTCT ACTCCTGTGA TCGCTATGGC AGCCGGGACC GATCCCAGCC CAAGTCTGCT 
GATCAGAGCA GGCCTACTTC ACCCAATGGT GGGCCTGAGC AAGGTCCCCA CAGACAGTGG 
TTCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134762.2
CDS	423..6971
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279276.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279276.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTATATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG ACTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGTT GCCGGATTCA TTATAAGGAT ATGTACAATT TGTTACGTGT AATTGCTCCA 
CCCCTGGGCT TAGGAAAGAA GTGCCCTCAT AGGGTGGCAT ACAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACA GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACACTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AACAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ATGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCGGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GAGGAGCAAA GCACGGTCAC CTGGGAGTTA CCTAGCACCC 
ATCCCGGACA CAAGCCCCAT GAAGCGCTCC ATCTCCACGC TGACCCCGCA GCGTCCTCAC 
CCCATGCACC TCTATGAGTA CTCCCTGGAG CGCGTGCCCA CGGACCAGGT GCATCACCAC 
CACCACCACC GCTGCCACCG GCGGAAAGAG AAGAAGCAGA AGTCCTTGGA CAGGACCACA 
CATCAGCTGG CCGATGGGGA AGCTGTGGCC CAGTCTGGTG AATCTTCTTC CAAGGACAAG 
AAGCAAGAGC GTGGACGGTC CCAAGAGAGG AAGCAGCACT CTTCCTCCTC CTCAGAAAAG 
CAGCGCTTCT ACTCCTGTGA TCGCTATGGC AGCCGGGACC GATCCCAGCC CAAGTCTGCT 
GATCAGAGCA GGCCTACTTC ACCCAATGGT GGGCCTGAGC AAGGTCCCCA CAGACAGTGG 
TTCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279277.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134763.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X20, mRNA.

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CloneID	OGa145088
Clone ID Related Accession (Same CDS sequence)	XM_015279277.2
Accession Version	XM_015279277.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCCAAGCAGC AGAACTCCTC CAAATCCAAG TCGGTTTGGG AGCAACGAAC CAGCCAGATC 
CGGATGCACA ACTTCCGAGC CAGCTGCGAG GCTCTGTACA ACGAACTGGA CCCCGAGGAG 
CGGGTGCGCT ACGCCACGAC CCTACACATC CGTCCAGACA TGAAGACCCA CTTGGATCGG 
CCACTGGTGG TAGAGCCAAG GGGTGAAGGG AGGAACAACA TCAGTAAGCT GAGCCCTGTG 
GATGTGCAGG AGGTGGAGCA GACCAAAGTC AGCTCTACCG ACGGAGCAGA AGCTCCGCGG 
AAGCATCACC GGCATCGGGA TAAGGAGAAA CTGGGAGAGC AAGAGAAGGG TGATGTGACC 
AAAGATGAGA ATGGGGAATC TGGCATCAAC AATAAGGAGG AGAGGCACAG GCAGCACAGA 
AGCCGTAGCA AGGAGGTGGA AGGGGGCAGC AAGGAAGGGA AAAGTGACCG CAGCAGGGGC 
CAGGAAGGAG GGAAGAGGCA CCATCGCCGG GGGTCGGTGG AAGAAGGCGT TGAGAAGGAG 
CACCGGAGGC ACCGCACGCA CCGGCACTCT GCAGAACGGC AAGGCAAGGA GGGCAATGGG 
ACCATCAACG GGGCCAGGAG TGAGCGGCGG ACCCGCCACC GTGGAGGGTC GAGGTCTGGG 
AACCGGGAAG GAGAGCCTGG GTCCAAGGGC GAGAATGGAG AGGAGCCTCA CAGACGGCAC 
CGGTTCAGGA GCAGGGCGCT GTCGACGTAC GATTCGGTGG AGAAGGAGAA CAGGGAGAAG 
GAAGGGGAGA CTGCTGAGAA GGAGCACCAG AATCATCAGC CCAAGGAGAA TCAGTGTGAG 
ATTGAGGCCA GTGGGAGTGT GAGCATCCCG GTGCACACCC TTCCCAGCAC CTATCTGCAG 
AAAGTGCCAG AGCAGCCTGA AGATGCTGAC AACCAGAAGA ACGTGACACG CATGATTCAG 
CCGCCTCTGG ACAAAACCAC CACTGTGAAC ATCCCCGTCA CCATCACCGC CCCGCCAGGA 
GAAACCACCG TCATACCTAT GAACAACGTT GAGTTCGAAA GTAAAACAGA AGAGAAGAAG 
GACGTTGATG ACTTGACGAA AAATGGGCCT AAACCAATCT TGCCTTACAG TTCCATGTTC 
ATCCTAAGTC CTACGAACCC GATCCGGCGT CTGTTCCACT ACATCGTCAA CCTGCGCTAC 
TTCGAGATGG TCATCCTCAT CGTCATCGCT CTCAGCAGCA TTGCCCTGGC AGCAGAAGAC 
CCCGTCCAAG CAGAATCACC AAGGAACGAT GCTCTGAAGT ACTTGGATTA TATATTCACG 
GGTGTCTTTA CCTTCGAGAT GGTGATCAAG ATGATTGACC TGGGGCTGTT ACTTCACCCT 
GGCTCCTATT TCCGTGACCT GTGGAACATC TTGGACTTCA TTGTGGTCAG TGGGGCCTTG 
GTGGCATTTG CTTTCTCAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTATATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGACT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGTTGCC GGATTCATTA TAAGGATATG 
TACAATTTGT TACGTGTAAT TGCTCCACCC CTGGGCTTAG GAAAGAAGTG CCCTCATAGG 
GTGGCATACA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGAG GAGCAAAGCA 
CGGTCACCTG GGAGTTACCT AGCACCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGTGGTTC GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134763.2
CDS	423..6938
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279277.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279277.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCCAAGCAGC AGAACTCCTC CAAATCCAAG TCGGTTTGGG AGCAACGAAC CAGCCAGATC 
CGGATGCACA ACTTCCGAGC CAGCTGCGAG GCTCTGTACA ACGAACTGGA CCCCGAGGAG 
CGGGTGCGCT ACGCCACGAC CCTACACATC CGTCCAGACA TGAAGACCCA CTTGGATCGG 
CCACTGGTGG TAGAGCCAAG GGGTGAAGGG AGGAACAACA TCAGTAAGCT GAGCCCTGTG 
GATGTGCAGG AGGTGGAGCA GACCAAAGTC AGCTCTACCG ACGGAGCAGA AGCTCCGCGG 
AAGCATCACC GGCATCGGGA TAAGGAGAAA CTGGGAGAGC AAGAGAAGGG TGATGTGACC 
AAAGATGAGA ATGGGGAATC TGGCATCAAC AATAAGGAGG AGAGGCACAG GCAGCACAGA 
AGCCGTAGCA AGGAGGTGGA AGGGGGCAGC AAGGAAGGGA AAAGTGACCG CAGCAGGGGC 
CAGGAAGGAG GGAAGAGGCA CCATCGCCGG GGGTCGGTGG AAGAAGGCGT TGAGAAGGAG 
CACCGGAGGC ACCGCACGCA CCGGCACTCT GCAGAACGGC AAGGCAAGGA GGGCAATGGG 
ACCATCAACG GGGCCAGGAG TGAGCGGCGG ACCCGCCACC GTGGAGGGTC GAGGTCTGGG 
AACCGGGAAG GAGAGCCTGG GTCCAAGGGC GAGAATGGAG AGGAGCCTCA CAGACGGCAC 
CGGTTCAGGA GCAGGGCGCT GTCGACGTAC GATTCGGTGG AGAAGGAGAA CAGGGAGAAG 
GAAGGGGAGA CTGCTGAGAA GGAGCACCAG AATCATCAGC CCAAGGAGAA TCAGTGTGAG 
ATTGAGGCCA GTGGGAGTGT GAGCATCCCG GTGCACACCC TTCCCAGCAC CTATCTGCAG 
AAAGTGCCAG AGCAGCCTGA AGATGCTGAC AACCAGAAGA ACGTGACACG CATGATTCAG 
CCGCCTCTGG ACAAAACCAC CACTGTGAAC ATCCCCGTCA CCATCACCGC CCCGCCAGGA 
GAAACCACCG TCATACCTAT GAACAACGTT GAGTTCGAAA GTAAAACAGA AGAGAAGAAG 
GACGTTGATG ACTTGACGAA AAATGGGCCT AAACCAATCT TGCCTTACAG TTCCATGTTC 
ATCCTAAGTC CTACGAACCC GATCCGGCGT CTGTTCCACT ACATCGTCAA CCTGCGCTAC 
TTCGAGATGG TCATCCTCAT CGTCATCGCT CTCAGCAGCA TTGCCCTGGC AGCAGAAGAC 
CCCGTCCAAG CAGAATCACC AAGGAACGAT GCTCTGAAGT ACTTGGATTA TATATTCACG 
GGTGTCTTTA CCTTCGAGAT GGTGATCAAG ATGATTGACC TGGGGCTGTT ACTTCACCCT 
GGCTCCTATT TCCGTGACCT GTGGAACATC TTGGACTTCA TTGTGGTCAG TGGGGCCTTG 
GTGGCATTTG CTTTCTCAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTATATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGACT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGTTGCC GGATTCATTA TAAGGATATG 
TACAATTTGT TACGTGTAAT TGCTCCACCC CTGGGCTTAG GAAAGAAGTG CCCTCATAGG 
GTGGCATACA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGAG GAGCAAAGCA 
CGGTCACCTG GGAGTTACCT AGCACCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGTGGTTC GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279278.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134764.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X21, mRNA.

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CloneID	OGa145089
Clone ID Related Accession (Same CDS sequence)	XM_015279278.2
Accession Version	XM_015279278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ATATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGACTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGTTGCCGG 
ATTCATTATA AGGATATGTA CAATTTGTTA CGTGTAATTG CTCCACCCCT GGGCTTAGGA 
AAGAAGTGCC CTCATAGGGT GGCATACAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGAGGA GCAAAGCACG GTCACCTGGG AGTTACCTAG CACCCATCCC GGACACAAGC 
CCCATGAAGC GCTCCATCTC CACGCTGACC CCGCAGCGTC CTCACCCCAT GCACCTCTAT 
GAGTACTCCC TGGAGCGCGT GCCCACGGAC CAGGTGCATC ACCACCACCA CCACCGCTGC 
CACCGGCGGA AAGAGAAGAA GCAGAAGTCC TTGGACAGGA CCACACATCA GCTGGCCGAT 
GGGGAAGCTG TGGCCCAGTC TGGTGAATCT TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA 
CGGTCCCAAG AGAGGAAGCA GCACTCTTCC TCCTCCTCAG AAAAGCAGCG CTTCTACTCC 
TGTGATCGCT ATGGCAGCCG GGACCGATCC CAGCCCAAGT CTGCTGATCA GAGCAGGCCT 
ACTTCACCCA ATGGTGGGCC TGAGCAAGGT CCCCACAGAC AGTGGTTCGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134764.2
CDS	423..6896
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279278.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279278.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ATATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGACTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGTTGCCGG 
ATTCATTATA AGGATATGTA CAATTTGTTA CGTGTAATTG CTCCACCCCT GGGCTTAGGA 
AAGAAGTGCC CTCATAGGGT GGCATACAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGAGGA GCAAAGCACG GTCACCTGGG AGTTACCTAG CACCCATCCC GGACACAAGC 
CCCATGAAGC GCTCCATCTC CACGCTGACC CCGCAGCGTC CTCACCCCAT GCACCTCTAT 
GAGTACTCCC TGGAGCGCGT GCCCACGGAC CAGGTGCATC ACCACCACCA CCACCGCTGC 
CACCGGCGGA AAGAGAAGAA GCAGAAGTCC TTGGACAGGA CCACACATCA GCTGGCCGAT 
GGGGAAGCTG TGGCCCAGTC TGGTGAATCT TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA 
CGGTCCCAAG AGAGGAAGCA GCACTCTTCC TCCTCCTCAG AAAAGCAGCG CTTCTACTCC 
TGTGATCGCT ATGGCAGCCG GGACCGATCC CAGCCCAAGT CTGCTGATCA GAGCAGGCCT 
ACTTCACCCA ATGGTGGGCC TGAGCAAGGT CCCCACAGAC AGTGGTTCGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279279.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134765.2 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X22, mRNA.

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CloneID	OGa145090
Clone ID Related Accession (Same CDS sequence)	XM_015279279.2
Accession Version	XM_015279279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCCAA GCAGCAGAAC 
TCCTCCAAAT CCAAGTCGGT TTGGGAGCAA CGAACCAGCC AGATCCGGAT GCACAACTTC 
CGAGCCAGCT GCGAGGCTCT GTACAACGAA CTGGACCCCG AGGAGCGGGT GCGCTACGCC 
ACGACCCTAC ACATCCGTCC AGACATGAAG ACCCACTTGG ATCGGCCACT GGTGGTAGAG 
CCAAGGGGTG AAGGGAGGAA CAACATCAGT AAGCTGAGCC CTGTGGATGT GCAGGAGGTG 
GAGCAGACCA AAGTCAGCTC TACCGACGGA GCAGAAGCTC CGCGGAAGCA TCACCGGCAT 
CGGGATAAGG AGAAACTGGG AGAGCAAGAG AAGGGTGATG TGACCAAAGA TGAGAATGGG 
GAATCTGGCA TCAACAATAA GGAGGAGAGG CACAGGCAGC ACAGAAGCCG TAGCAAGGAG 
GTGGAAGGGG GCAGCAAGGA AGGGAAAAGT GACCGCAGCA GGGGCCAGGA AGGAGGGAAG 
AGGCACCATC GCCGGGGGTC GGTGGAAGAA GGCGTTGAGA AGGAGCACCG GAGGCACCGC 
ACGCACCGGC ACTCTGCAGA ACGGCAAGGC AAGGAGGGCA ATGGGACCAT CAACGGGGCC 
AGGAGTGAGC GGCGGACCCG CCACCGTGGA GGGTCGAGGT CTGGGAACCG GGAAGGAGAG 
CCTGGGTCCA AGGGCGAGAA TGGAGAGGAG CCTCACAGAC GGCACCGGTT CAGGAGCAGG 
GCGCTGTCGA CGTACGATTC GGTGGAGAAG GAGAACAGGG AGAAGGAAGG GGAGACTGCT 
GAGAAGGAGC ACCAGAATCA TCAGCCCAAG GAGAATCAGT GTGAGATTGA GGCCAGTGGG 
AGTGTGAGCA TCCCGGTGCA CACCCTTCCC AGCACCTATC TGCAGAAAGT GCCAGAGCAG 
CCTGAAGATG CTGACAACCA GAAGAACGTG ACACGCATGA TTCAGCCGCC TCTGGACAAA 
ACCACCACTG TGAACATCCC CGTCACCATC ACCGCCCCGC CAGGAGAAAC CACCGTCATA 
CCTATGAACA ACGTTGAGTT CGAAAGTAAA ACAGAAGAGA AGAAGGACGT TGATGACTTG 
ACGAAAAATG GGCCTAAACC AATCTTGCCT TACAGTTCCA TGTTCATCCT AAGTCCTACG 
AACCCGATCC GGCGTCTGTT CCACTACATC GTCAACCTGC GCTACTTCGA GATGGTCATC 
CTCATCGTCA TCGCTCTCAG CAGCATTGCC CTGGCAGCAG AAGACCCCGT CCAAGCAGAA 
TCACCAAGGA ACGATGCTCT GAAGTACTTG GATTATATAT TCACGGGTGT CTTTACCTTC 
GAGATGGTGA TCAAGATGAT TGACCTGGGG CTGTTACTTC ACCCTGGCTC CTATTTCCGT 
GACCTGTGGA ACATCTTGGA CTTCATTGTG GTCAGTGGGG CCTTGGTGGC ATTTGCTTTC 
TCAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTATA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACACGGACA ACTTCATCAA CCTCAGCTTC CTGCGGCTCT TCAGGGCAGC AAGACTTATC 
AAGCTCCTTC GCCAGGGTTA CACTATCCGC ATCTTGCTCT GGACGTTTGT GCAGTCATTT 
AAGGCCTTGC CTTATGTGTG TCTTCTCATT GCAATGCTCT TCTTCATCTA TGCCATTATT 
GGCATGCAGG TATTTGGAAA CATTGCACTA AATGACGAAA CCTCCATCAA TCGGCACAAT 
AATTTCCGTA CCTTCCTTCA AGCCTTGATG CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC ATGCCTGAGC AACAGGGCCT GCGACCCACT CTCCGGCCTC 
ACCAAGAACG AGTGCGGCAG CGACTTCGCC TACTTCTACT TCGTGTCATT CATCTTCCTC 
TGCTCCTTCC TGATGTTGAA CCTATTTGTG GCTGTAATCA TGGACAACTT TGAATACTTG 
ACAAGAGACT CCTCCATCCT TGGGCCCCAT CATTTGGATG AGTTTGTCCG TGTCTGGGCT 
GAATACGACC CAGCTGCCTG TTGCCGGATT CATTATAAGG ATATGTACAA TTTGTTACGT 
GTAATTGCTC CACCCCTGGG CTTAGGAAAG AAGTGCCCTC ATAGGGTGGC ATACAAGCGC 
CTGGTGCGGA TGAACATGCC CATCTCACCC GAGGATCTGA CAGTCCACTT CACCTCCACG 
CTGATGGCCC TGATCCGCAC TGCTCTGGAA ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAGGAG ATATCTCTAG TTTGGCCCAA CCTGTCCCAG 
AAGACACTGG ATTTGCTGGT GCCTCCTCAC AAACCTGATG AGATGACTGT GGGGAAGGTC 
TACGCAGCGC TCATGATATT TGACTTCTAC AAACAGAACA AGAACAGCAG GGAACAAGTC 
CACCAGCCTC CTGGGGGCCT GTGCCAGCCC GGACCAGTGT CACTCTTCCA CCCCTTGAAG 
GCCACTTTGG AGCAAACCCA GCCCTCGGCA TTCAGCAGTG CGAAGGCATT CCTGCGCCAG 
AAAAGCTCGG CCTCACTCAA CAATGGAGGC ACATTGCCAG CCCCAGAGGG TGGGATCAAA 
GAGTCCAGTT CGTGGGGGAC CCAACGGACC CAGGACGTGT TTTATGAAAC CAGGACACCA 
GCTTTTGAAC GAGGCCACTC AGAGGAAATC CCCATTGAAC GGGTGGTGGA AATGAGGGAA 
ATCAGCCCCA CACTTGCCAA TGGAGAGCAC CAACCAGGGC TGGAGAGCCA GGGTCGGGCG 
GCTTCCATGC CGCGCCTGGC TGCTGAAACG CAGAGGAGCA AAGCACGGTC ACCTGGGAGT 
TACCTAGCAC CCATCCCGGA CACAAGCCCC ATGAAGCGCT CCATCTCCAC GCTGACCCCG 
CAGCGTCCTC ACCCCATGCA CCTCTATGAG TACTCCCTGG AGCGCGTGCC CACGGACCAG 
GTGCATCACC ACCACCACCA CCGCTGCCAC CGGCGGAAAG AGAAGAAGCA GAAGTCCTTG 
GACAGGACCA CACATCAGCT GGCCGATGGG GAAGCTGTGG CCCAGTCTGG TGAATCTTCT 
TCCAAGGACA AGAAGCAAGA GCGTGGACGG TCCCAAGAGA GGAAGCAGCA CTCTTCCTCC 
TCCTCAGAAA AGCAGCGCTT CTACTCCTGT GATCGCTATG GCAGCCGGGA CCGATCCCAG 
CCCAAGTCTG CTGATCAGAG CAGGCCTACT TCACCCAATG GTGGGCCTGA GCAAGGTCCC 
CACAGACAGT GGTTCGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134765.2
CDS	423..6863
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279279.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279279.2

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCCAA GCAGCAGAAC 
TCCTCCAAAT CCAAGTCGGT TTGGGAGCAA CGAACCAGCC AGATCCGGAT GCACAACTTC 
CGAGCCAGCT GCGAGGCTCT GTACAACGAA CTGGACCCCG AGGAGCGGGT GCGCTACGCC 
ACGACCCTAC ACATCCGTCC AGACATGAAG ACCCACTTGG ATCGGCCACT GGTGGTAGAG 
CCAAGGGGTG AAGGGAGGAA CAACATCAGT AAGCTGAGCC CTGTGGATGT GCAGGAGGTG 
GAGCAGACCA AAGTCAGCTC TACCGACGGA GCAGAAGCTC CGCGGAAGCA TCACCGGCAT 
CGGGATAAGG AGAAACTGGG AGAGCAAGAG AAGGGTGATG TGACCAAAGA TGAGAATGGG 
GAATCTGGCA TCAACAATAA GGAGGAGAGG CACAGGCAGC ACAGAAGCCG TAGCAAGGAG 
GTGGAAGGGG GCAGCAAGGA AGGGAAAAGT GACCGCAGCA GGGGCCAGGA AGGAGGGAAG 
AGGCACCATC GCCGGGGGTC GGTGGAAGAA GGCGTTGAGA AGGAGCACCG GAGGCACCGC 
ACGCACCGGC ACTCTGCAGA ACGGCAAGGC AAGGAGGGCA ATGGGACCAT CAACGGGGCC 
AGGAGTGAGC GGCGGACCCG CCACCGTGGA GGGTCGAGGT CTGGGAACCG GGAAGGAGAG 
CCTGGGTCCA AGGGCGAGAA TGGAGAGGAG CCTCACAGAC GGCACCGGTT CAGGAGCAGG 
GCGCTGTCGA CGTACGATTC GGTGGAGAAG GAGAACAGGG AGAAGGAAGG GGAGACTGCT 
GAGAAGGAGC ACCAGAATCA TCAGCCCAAG GAGAATCAGT GTGAGATTGA GGCCAGTGGG 
AGTGTGAGCA TCCCGGTGCA CACCCTTCCC AGCACCTATC TGCAGAAAGT GCCAGAGCAG 
CCTGAAGATG CTGACAACCA GAAGAACGTG ACACGCATGA TTCAGCCGCC TCTGGACAAA 
ACCACCACTG TGAACATCCC CGTCACCATC ACCGCCCCGC CAGGAGAAAC CACCGTCATA 
CCTATGAACA ACGTTGAGTT CGAAAGTAAA ACAGAAGAGA AGAAGGACGT TGATGACTTG 
ACGAAAAATG GGCCTAAACC AATCTTGCCT TACAGTTCCA TGTTCATCCT AAGTCCTACG 
AACCCGATCC GGCGTCTGTT CCACTACATC GTCAACCTGC GCTACTTCGA GATGGTCATC 
CTCATCGTCA TCGCTCTCAG CAGCATTGCC CTGGCAGCAG AAGACCCCGT CCAAGCAGAA 
TCACCAAGGA ACGATGCTCT GAAGTACTTG GATTATATAT TCACGGGTGT CTTTACCTTC 
GAGATGGTGA TCAAGATGAT TGACCTGGGG CTGTTACTTC ACCCTGGCTC CTATTTCCGT 
GACCTGTGGA ACATCTTGGA CTTCATTGTG GTCAGTGGGG CCTTGGTGGC ATTTGCTTTC 
TCAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTATA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACACGGACA ACTTCATCAA CCTCAGCTTC CTGCGGCTCT TCAGGGCAGC AAGACTTATC 
AAGCTCCTTC GCCAGGGTTA CACTATCCGC ATCTTGCTCT GGACGTTTGT GCAGTCATTT 
AAGGCCTTGC CTTATGTGTG TCTTCTCATT GCAATGCTCT TCTTCATCTA TGCCATTATT 
GGCATGCAGG TATTTGGAAA CATTGCACTA AATGACGAAA CCTCCATCAA TCGGCACAAT 
AATTTCCGTA CCTTCCTTCA AGCCTTGATG CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC ATGCCTGAGC AACAGGGCCT GCGACCCACT CTCCGGCCTC 
ACCAAGAACG AGTGCGGCAG CGACTTCGCC TACTTCTACT TCGTGTCATT CATCTTCCTC 
TGCTCCTTCC TGATGTTGAA CCTATTTGTG GCTGTAATCA TGGACAACTT TGAATACTTG 
ACAAGAGACT CCTCCATCCT TGGGCCCCAT CATTTGGATG AGTTTGTCCG TGTCTGGGCT 
GAATACGACC CAGCTGCCTG TTGCCGGATT CATTATAAGG ATATGTACAA TTTGTTACGT 
GTAATTGCTC CACCCCTGGG CTTAGGAAAG AAGTGCCCTC ATAGGGTGGC ATACAAGCGC 
CTGGTGCGGA TGAACATGCC CATCTCACCC GAGGATCTGA CAGTCCACTT CACCTCCACG 
CTGATGGCCC TGATCCGCAC TGCTCTGGAA ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAGGAG ATATCTCTAG TTTGGCCCAA CCTGTCCCAG 
AAGACACTGG ATTTGCTGGT GCCTCCTCAC AAACCTGATG AGATGACTGT GGGGAAGGTC 
TACGCAGCGC TCATGATATT TGACTTCTAC AAACAGAACA AGAACAGCAG GGAACAAGTC 
CACCAGCCTC CTGGGGGCCT GTGCCAGCCC GGACCAGTGT CACTCTTCCA CCCCTTGAAG 
GCCACTTTGG AGCAAACCCA GCCCTCGGCA TTCAGCAGTG CGAAGGCATT CCTGCGCCAG 
AAAAGCTCGG CCTCACTCAA CAATGGAGGC ACATTGCCAG CCCCAGAGGG TGGGATCAAA 
GAGTCCAGTT CGTGGGGGAC CCAACGGACC CAGGACGTGT TTTATGAAAC CAGGACACCA 
GCTTTTGAAC GAGGCCACTC AGAGGAAATC CCCATTGAAC GGGTGGTGGA AATGAGGGAA 
ATCAGCCCCA CACTTGCCAA TGGAGAGCAC CAACCAGGGC TGGAGAGCCA GGGTCGGGCG 
GCTTCCATGC CGCGCCTGGC TGCTGAAACG CAGAGGAGCA AAGCACGGTC ACCTGGGAGT 
TACCTAGCAC CCATCCCGGA CACAAGCCCC ATGAAGCGCT CCATCTCCAC GCTGACCCCG 
CAGCGTCCTC ACCCCATGCA CCTCTATGAG TACTCCCTGG AGCGCGTGCC CACGGACCAG 
GTGCATCACC ACCACCACCA CCGCTGCCAC CGGCGGAAAG AGAAGAAGCA GAAGTCCTTG 
GACAGGACCA CACATCAGCT GGCCGATGGG GAAGCTGTGG CCCAGTCTGG TGAATCTTCT 
TCCAAGGACA AGAAGCAAGA GCGTGGACGG TCCCAAGAGA GGAAGCAGCA CTCTTCCTCC 
TCCTCAGAAA AGCAGCGCTT CTACTCCTGT GATCGCTATG GCAGCCGGGA CCGATCCCAG 
CCCAAGTCTG CTGATCAGAG CAGGCCTACT TCACCCAATG GTGGGCCTGA GCAAGGTCCC 
CACAGACAGT GGTTCGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279261.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134747.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X1, mRNA.

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CloneID	OGa43287
Clone ID Related Accession (Same CDS sequence)	XM_015279261.1
Accession Version	XM_015279261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ACATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGAGTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGCGGGCGC ATCAGTTACA CAGACATGTA TGAGATGCTG 
AGACACATGT CCCCACCTCT AGGCCTTGGA AAGAAATGCC CTGCTCGCGT TGCCTATAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGGGCA GTGGTTCGGT TAACGGGAGC 
CCCTTGCTGT CGACATCTGG TGCTAGTACC CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG 
ACCCCCCTGA CCCCACGCCC CAGCATCACT TACAAGACCG CCAACTCCTC GCCCGTACAC 
TTCAGCACGT CGCCGGGCGG CCTCCCGCCC TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG 
TCTGAGCACA ATGCACTGCT GCGTGGGGAC CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC 
ATCGGCTCCG ACCCTTACCT GGGCCACCGC GACGCCGCCG ACAGCCCCAT AGGCGCAGCG 
CCCGAGGACA CGCTCACCTT CGAGGAAGCC GTGGCCACCA ACTCGGGGCG TTCCTCCAGG 
ACTTCTTACG TCTCCTCCTT GACCTCCCAG TCTCACCAAG CACGCCGGGT GCCCAATGGC 
TACCATTACA CGCTGGGGCT CAACACGGGG CCCGGCACTG GCACCAGGGG ACGGAGCTAC 
TACCACGAAG CCGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134747.1
CDS	425..7537
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279261.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279261.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ACATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGAGTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGCGGGCGC ATCAGTTACA CAGACATGTA TGAGATGCTG 
AGACACATGT CCCCACCTCT AGGCCTTGGA AAGAAATGCC CTGCTCGCGT TGCCTATAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGGGCA GTGGTTCGGT TAACGGGAGC 
CCCTTGCTGT CGACATCTGG TGCTAGTACC CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG 
ACCCCCCTGA CCCCACGCCC CAGCATCACT TACAAGACCG CCAACTCCTC GCCCGTACAC 
TTCAGCACGT CGCCGGGCGG CCTCCCGCCC TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG 
TCTGAGCACA ATGCACTGCT GCGTGGGGAC CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC 
ATCGGCTCCG ACCCTTACCT GGGCCACCGC GACGCCGCCG ACAGCCCCAT AGGCGCAGCG 
CCCGAGGACA CGCTCACCTT CGAGGAAGCC GTGGCCACCA ACTCGGGGCG TTCCTCCAGG 
ACTTCTTACG TCTCCTCCTT GACCTCCCAG TCTCACCAAG CACGCCGGGT GCCCAATGGC 
TACCATTACA CGCTGGGGCT CAACACGGGG CCCGGCACTG GCACCAGGGG ACGGAGCTAC 
TACCACGAAG CCGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279262.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134748.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X3, mRNA.

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CloneID	OGa43288
Clone ID Related Accession (Same CDS sequence)	XM_015279262.1
Accession Version	XM_015279262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ACATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGAGTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGTTGCCGG ATTCATTATA AGGATATGTA CAATTTGTTA 
CGTGTAATTG CTCCACCCCT GGGCTTAGGA AAGAAGTGCC CTCATAGGGT GGCATACAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGGGCA GTGGTTCGGT TAACGGGAGC 
CCCTTGCTGT CGACATCTGG TGCTAGTACC CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG 
ACCCCCCTGA CCCCACGCCC CAGCATCACT TACAAGACCG CCAACTCCTC GCCCGTACAC 
TTCAGCACGT CGCCGGGCGG CCTCCCGCCC TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG 
TCTGAGCACA ATGCACTGCT GCGTGGGGAC CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC 
ATCGGCTCCG ACCCTTACCT GGGCCACCGC GACGCCGCCG ACAGCCCCAT AGGCGCAGCG 
CCCGAGGACA CGCTCACCTT CGAGGAAGCC GTGGCCACCA ACTCGGGGCG TTCCTCCAGG 
ACTTCTTACG TCTCCTCCTT GACCTCCCAG TCTCACCAAG CACGCCGGGT GCCCAATGGC 
TACCATTACA CGCTGGGGCT CAACACGGGG CCCGGCACTG GCACCAGGGG ACGGAGCTAC 
TACCACGAAG CCGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134748.1
CDS	427..7539
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279262.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279262.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ACATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGAGTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGTTGCCGG ATTCATTATA AGGATATGTA CAATTTGTTA 
CGTGTAATTG CTCCACCCCT GGGCTTAGGA AAGAAGTGCC CTCATAGGGT GGCATACAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGGGCA GTGGTTCGGT TAACGGGAGC 
CCCTTGCTGT CGACATCTGG TGCTAGTACC CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG 
ACCCCCCTGA CCCCACGCCC CAGCATCACT TACAAGACCG CCAACTCCTC GCCCGTACAC 
TTCAGCACGT CGCCGGGCGG CCTCCCGCCC TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG 
TCTGAGCACA ATGCACTGCT GCGTGGGGAC CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC 
ATCGGCTCCG ACCCTTACCT GGGCCACCGC GACGCCGCCG ACAGCCCCAT AGGCGCAGCG 
CCCGAGGACA CGCTCACCTT CGAGGAAGCC GTGGCCACCA ACTCGGGGCG TTCCTCCAGG 
ACTTCTTACG TCTCCTCCTT GACCTCCCAG TCTCACCAAG CACGCCGGGT GCCCAATGGC 
TACCATTACA CGCTGGGGCT CAACACGGGG CCCGGCACTG GCACCAGGGG ACGGAGCTAC 
TACCACGAAG CCGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279263.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134749.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X4, mRNA.

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CloneID	OGa43289
Clone ID Related Accession (Same CDS sequence)	XM_015279263.1
Accession Version	XM_015279263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ACATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACAACT TCATCAACCT CAGCTTCCTG CGGCTCTTCA GGGCAGCAAG ACTTATCAAG 
CTCCTTCGCC AGGGTTACAC TATCCGCATC TTGCTCTGGA CGTTTGTGCA GTCATTTAAG 
GCCTTGCCTT ATGTGTGTCT TCTCATTGCA ATGCTCTTCT TCATCTATGC CATTATTGGC 
ATGCAGGTAT TTGGAAACAT TGCACTAAAT GACGAAACCT CCATCAATCG GCACAATAAT 
TTCCGTACCT TCCTTCAAGC CTTGATGCTT CTGTTCAGGA GTGCCACGGG GGAGGCCTGG 
CACGAGATCA TGCTGTCATG CCTGAGCAAC AGGGCCTGCG ACCCACTCTC CGGCCTCACC 
AAGAACGAGT GCGGCAGCGA GTTCGCCTAC TTCTACTTCG TGTCATTCAT CTTCCTCTGC 
TCCTTCCTGA TGTTGAACCT ATTTGTGGCT GTAATCATGG ACAACTTTGA ATACTTGACA 
AGAGACTCCT CCATCCTTGG GCCCCATCAT TTGGATGAGT TTGTCCGTGT CTGGGCTGAA 
TACGACCCAG CTGCCTGCGG GCGCATCAGT TACACAGACA TGTATGAGAT GCTGAGACAC 
ATGTCCCCAC CTCTAGGCCT TGGAAAGAAA TGCCCTGCTC GCGTTGCCTA TAAGCGCCTG 
GTGCGGATGA ACATGCCCAT CTCACCCGAG GATCTGACAG TCCACTTCAC CTCCACGCTG 
ATGGCCCTGA TCCGCACTGC TCTGGAAATC AAGCTCGCCT CAGGGGGTGT TAAGCAGCAC 
CAGTGTGATG CTGAGCTGAG AAAGGAGATA TCTCTAGTTT GGCCCAACCT GTCCCAGAAG 
ACACTGGATT TGCTGGTGCC TCCTCACAAA CCTGATGAGA TGACTGTGGG GAAGGTCTAC 
GCAGCGCTCA TGATATTTGA CTTCTACAAA CAGAACAAGA ACAGCAGGGA ACAAGTCCAC 
CAGCCTCCTG GGGGCCTGTG CCAGCCCGGA CCAGTGTCAC TCTTCCACCC CTTGAAGGCC 
ACTTTGGAGC AAACCCAGCC CTCGGCATTC AGCAGTGCGA AGGCATTCCT GCGCCAGAAA 
AGCTCGGCCT CACTCAACAA TGGAGGCACA TTGCCAGCCC CAGAGGGTGG GATCAAAGAG 
TCCAGTTCGT GGGGGACCCA ACGGACCCAG GACGTGTTTT ATGAAACCAG GACACCAGCT 
TTTGAACGAG GCCACTCAGA GGAAATCCCC ATTGAACGGG TGGTGGAAAT GAGGGAAATC 
AGCCCCACAC TTGCCAATGG AGAGCACCAA CCAGGGCTGG AGAGCCAGGG TCGGGCGGCT 
TCCATGCCGC GCCTGGCTGC TGAAACGCAG CCCATCCCGG ACACAAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGACCCC GCAGCGTCCT CACCCCATGC ACCTCTATGA GTACTCCCTG 
GAGCGCGTGC CCACGGACCA GGTGCATCAC CACCACCACC ACCGCTGCCA CCGGCGGAAA 
GAGAAGAAGC AGAAGTCCTT GGACAGGACC ACACATCAGC TGGCCGATGG GGAAGCTGTG 
GCCCAGTCTG GTGAATCTTC TTCCAAGGAC AAGAAGCAAG AGCGTGGACG GTCCCAAGAG 
AGGAAGCAGC ACTCTTCCTC CTCCTCAGAA AAGCAGCGCT TCTACTCCTG TGATCGCTAT 
GGCAGCCGGG ACCGATCCCA GCCCAAGTCT GCTGATCAGA GCAGGCCTAC TTCACCCAAT 
GGTGGGCCTG AGCAAGGTCC CCACAGACAG GGCAGTGGTT CGGTTAACGG GAGCCCCTTG 
CTGTCGACAT CTGGTGCTAG TACCCCCTGC CGGGGTCGGA GGCAGCTCCC GCAGACCCCC 
CTGACCCCAC GCCCCAGCAT CACTTACAAG ACCGCCAACT CCTCGCCCGT ACACTTCAGC 
ACGTCGCCGG GCGGCCTCCC GCCCTTCTCC CCGGGCCGCC TGAGCCGCGG GCTGTCTGAG 
CACAATGCAC TGCTGCGTGG GGACCAGCAG CCGCCCCCGG CCGCGGTTGC CCGCATCGGC 
TCCGACCCTT ACCTGGGCCA CCGCGACGCC GCCGACAGCC CCATAGGCGC AGCGCCCGAG 
GACACGCTCA CCTTCGAGGA AGCCGTGGCC ACCAACTCGG GGCGTTCCTC CAGGACTTCT 
TACGTCTCCT CCTTGACCTC CCAGTCTCAC CAAGCACGCC GGGTGCCCAA TGGCTACCAT 
TACACGCTGG GGCTCAACAC GGGGCCCGGC ACTGGCACCA GGGGACGGAG CTACTACCAC 
GAAGCCGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134749.1
CDS	425..7531
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279263.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279263.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ACATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACAACT TCATCAACCT CAGCTTCCTG CGGCTCTTCA GGGCAGCAAG ACTTATCAAG 
CTCCTTCGCC AGGGTTACAC TATCCGCATC TTGCTCTGGA CGTTTGTGCA GTCATTTAAG 
GCCTTGCCTT ATGTGTGTCT TCTCATTGCA ATGCTCTTCT TCATCTATGC CATTATTGGC 
ATGCAGGTAT TTGGAAACAT TGCACTAAAT GACGAAACCT CCATCAATCG GCACAATAAT 
TTCCGTACCT TCCTTCAAGC CTTGATGCTT CTGTTCAGGA GTGCCACGGG GGAGGCCTGG 
CACGAGATCA TGCTGTCATG CCTGAGCAAC AGGGCCTGCG ACCCACTCTC CGGCCTCACC 
AAGAACGAGT GCGGCAGCGA GTTCGCCTAC TTCTACTTCG TGTCATTCAT CTTCCTCTGC 
TCCTTCCTGA TGTTGAACCT ATTTGTGGCT GTAATCATGG ACAACTTTGA ATACTTGACA 
AGAGACTCCT CCATCCTTGG GCCCCATCAT TTGGATGAGT TTGTCCGTGT CTGGGCTGAA 
TACGACCCAG CTGCCTGCGG GCGCATCAGT TACACAGACA TGTATGAGAT GCTGAGACAC 
ATGTCCCCAC CTCTAGGCCT TGGAAAGAAA TGCCCTGCTC GCGTTGCCTA TAAGCGCCTG 
GTGCGGATGA ACATGCCCAT CTCACCCGAG GATCTGACAG TCCACTTCAC CTCCACGCTG 
ATGGCCCTGA TCCGCACTGC TCTGGAAATC AAGCTCGCCT CAGGGGGTGT TAAGCAGCAC 
CAGTGTGATG CTGAGCTGAG AAAGGAGATA TCTCTAGTTT GGCCCAACCT GTCCCAGAAG 
ACACTGGATT TGCTGGTGCC TCCTCACAAA CCTGATGAGA TGACTGTGGG GAAGGTCTAC 
GCAGCGCTCA TGATATTTGA CTTCTACAAA CAGAACAAGA ACAGCAGGGA ACAAGTCCAC 
CAGCCTCCTG GGGGCCTGTG CCAGCCCGGA CCAGTGTCAC TCTTCCACCC CTTGAAGGCC 
ACTTTGGAGC AAACCCAGCC CTCGGCATTC AGCAGTGCGA AGGCATTCCT GCGCCAGAAA 
AGCTCGGCCT CACTCAACAA TGGAGGCACA TTGCCAGCCC CAGAGGGTGG GATCAAAGAG 
TCCAGTTCGT GGGGGACCCA ACGGACCCAG GACGTGTTTT ATGAAACCAG GACACCAGCT 
TTTGAACGAG GCCACTCAGA GGAAATCCCC ATTGAACGGG TGGTGGAAAT GAGGGAAATC 
AGCCCCACAC TTGCCAATGG AGAGCACCAA CCAGGGCTGG AGAGCCAGGG TCGGGCGGCT 
TCCATGCCGC GCCTGGCTGC TGAAACGCAG CCCATCCCGG ACACAAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGACCCC GCAGCGTCCT CACCCCATGC ACCTCTATGA GTACTCCCTG 
GAGCGCGTGC CCACGGACCA GGTGCATCAC CACCACCACC ACCGCTGCCA CCGGCGGAAA 
GAGAAGAAGC AGAAGTCCTT GGACAGGACC ACACATCAGC TGGCCGATGG GGAAGCTGTG 
GCCCAGTCTG GTGAATCTTC TTCCAAGGAC AAGAAGCAAG AGCGTGGACG GTCCCAAGAG 
AGGAAGCAGC ACTCTTCCTC CTCCTCAGAA AAGCAGCGCT TCTACTCCTG TGATCGCTAT 
GGCAGCCGGG ACCGATCCCA GCCCAAGTCT GCTGATCAGA GCAGGCCTAC TTCACCCAAT 
GGTGGGCCTG AGCAAGGTCC CCACAGACAG GGCAGTGGTT CGGTTAACGG GAGCCCCTTG 
CTGTCGACAT CTGGTGCTAG TACCCCCTGC CGGGGTCGGA GGCAGCTCCC GCAGACCCCC 
CTGACCCCAC GCCCCAGCAT CACTTACAAG ACCGCCAACT CCTCGCCCGT ACACTTCAGC 
ACGTCGCCGG GCGGCCTCCC GCCCTTCTCC CCGGGCCGCC TGAGCCGCGG GCTGTCTGAG 
CACAATGCAC TGCTGCGTGG GGACCAGCAG CCGCCCCCGG CCGCGGTTGC CCGCATCGGC 
TCCGACCCTT ACCTGGGCCA CCGCGACGCC GCCGACAGCC CCATAGGCGC AGCGCCCGAG 
GACACGCTCA CCTTCGAGGA AGCCGTGGCC ACCAACTCGG GGCGTTCCTC CAGGACTTCT 
TACGTCTCCT CCTTGACCTC CCAGTCTCAC CAAGCACGCC GGGTGCCCAA TGGCTACCAT 
TACACGCTGG GGCTCAACAC GGGGCCCGGC ACTGGCACCA GGGGACGGAG CTACTACCAC 
GAAGCCGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279264.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134750.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X5, mRNA.

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CloneID	OGa43290
Clone ID Related Accession (Same CDS sequence)	XM_015279264.1
Accession Version	XM_015279264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACACGGAC 
AACTTCATCA ACCTCAGCTT CCTGCGGCTC TTCAGGGCAG CAAGACTTAT CAAGCTCCTT 
CGCCAGGGTT ACACTATCCG CATCTTGCTC TGGACGTTTG TGCAGTCATT TAAGGCCTTG 
CCTTATGTGT GTCTTCTCAT TGCAATGCTC TTCTTCATCT ATGCCATTAT TGGCATGCAG 
GTATTTGGAA ACATTGCACT AAATGACGAA ACCTCCATCA ATCGGCACAA TAATTTCCGT 
ACCTTCCTTC AAGCCTTGAT GCTTCTGTTC AGGAGTGCCA CGGGGGAGGC CTGGCACGAG 
ATCATGCTGT CATGCCTGAG CAACAGGGCC TGCGACCCAC TCTCCGGCCT CACCAAGAAC 
GAGTGCGGCA GCGAGTTCGC CTACTTCTAC TTCGTGTCAT TCATCTTCCT CTGCTCCTTC 
CTGATGTTGA ACCTATTTGT GGCTGTAATC ATGGACAACT TTGAATACTT GACAAGAGAC 
TCCTCCATCC TTGGGCCCCA TCATTTGGAT GAGTTTGTCC GTGTCTGGGC TGAATACGAC 
CCAGCTGCCT GCGGGCGCAT CAGTTACACA GACATGTATG AGATGCTGAG ACACATGTCC 
CCACCTCTAG GCCTTGGAAA GAAATGCCCT GCTCGCGTTG CCTATAAGCG CCTGGTGCGG 
ATGAACATGC CCATCTCACC CGAGGATCTG ACAGTCCACT TCACCTCCAC GCTGATGGCC 
CTGATCCGCA CTGCTCTGGA AATCAAGCTC GCCTCAGGGG GTGTTAAGCA GCACCAGTGT 
GATGCTGAGC TGAGAAAGGA GATATCTCTA GTTTGGCCCA ACCTGTCCCA GAAGACACTG 
GATTTGCTGG TGCCTCCTCA CAAACCTGAT GAGATGACTG TGGGGAAGGT CTACGCAGCG 
CTCATGATAT TTGACTTCTA CAAACAGAAC AAGAACAGCA GGGAACAAGT CCACCAGCCT 
CCTGGGGGCC TGTGCCAGCC CGGACCAGTG TCACTCTTCC ACCCCTTGAA GGCCACTTTG 
GAGCAAACCC AGCCCTCGGC ATTCAGCAGT GCGAAGGCAT TCCTGCGCCA GAAAAGCTCG 
GCCTCACTCA ACAATGGAGG CACATTGCCA GCCCCAGAGG GTGGGATCAA AGAGTCCAGT 
TCGTGGGGGA CCCAACGGAC CCAGGACGTG TTTTATGAAA CCAGGACACC AGCTTTTGAA 
CGAGGCCACT CAGAGGAAAT CCCCATTGAA CGGGTGGTGG AAATGAGGGA AATCAGCCCC 
ACACTTGCCA ATGGAGAGCA CCAACCAGGG CTGGAGAGCC AGGGTCGGGC GGCTTCCATG 
CCGCGCCTGG CTGCTGAAAC GCAGCCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGGGCAGT GGTTCGGTTA ACGGGAGCCC CTTGCTGTCG 
ACATCTGGTG CTAGTACCCC CTGCCGGGGT CGGAGGCAGC TCCCGCAGAC CCCCCTGACC 
CCACGCCCCA GCATCACTTA CAAGACCGCC AACTCCTCGC CCGTACACTT CAGCACGTCG 
CCGGGCGGCC TCCCGCCCTT CTCCCCGGGC CGCCTGAGCC GCGGGCTGTC TGAGCACAAT 
GCACTGCTGC GTGGGGACCA GCAGCCGCCC CCGGCCGCGG TTGCCCGCAT CGGCTCCGAC 
CCTTACCTGG GCCACCGCGA CGCCGCCGAC AGCCCCATAG GCGCAGCGCC CGAGGACACG 
CTCACCTTCG AGGAAGCCGT GGCCACCAAC TCGGGGCGTT CCTCCAGGAC TTCTTACGTC 
TCCTCCTTGA CCTCCCAGTC TCACCAAGCA CGCCGGGTGC CCAATGGCTA CCATTACACG 
CTGGGGCTCA ACACGGGGCC CGGCACTGGC ACCAGGGGAC GGAGCTACTA CCACGAAGCC 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134750.1
CDS	425..7525
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279264.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279264.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACACGGAC 
AACTTCATCA ACCTCAGCTT CCTGCGGCTC TTCAGGGCAG CAAGACTTAT CAAGCTCCTT 
CGCCAGGGTT ACACTATCCG CATCTTGCTC TGGACGTTTG TGCAGTCATT TAAGGCCTTG 
CCTTATGTGT GTCTTCTCAT TGCAATGCTC TTCTTCATCT ATGCCATTAT TGGCATGCAG 
GTATTTGGAA ACATTGCACT AAATGACGAA ACCTCCATCA ATCGGCACAA TAATTTCCGT 
ACCTTCCTTC AAGCCTTGAT GCTTCTGTTC AGGAGTGCCA CGGGGGAGGC CTGGCACGAG 
ATCATGCTGT CATGCCTGAG CAACAGGGCC TGCGACCCAC TCTCCGGCCT CACCAAGAAC 
GAGTGCGGCA GCGAGTTCGC CTACTTCTAC TTCGTGTCAT TCATCTTCCT CTGCTCCTTC 
CTGATGTTGA ACCTATTTGT GGCTGTAATC ATGGACAACT TTGAATACTT GACAAGAGAC 
TCCTCCATCC TTGGGCCCCA TCATTTGGAT GAGTTTGTCC GTGTCTGGGC TGAATACGAC 
CCAGCTGCCT GCGGGCGCAT CAGTTACACA GACATGTATG AGATGCTGAG ACACATGTCC 
CCACCTCTAG GCCTTGGAAA GAAATGCCCT GCTCGCGTTG CCTATAAGCG CCTGGTGCGG 
ATGAACATGC CCATCTCACC CGAGGATCTG ACAGTCCACT TCACCTCCAC GCTGATGGCC 
CTGATCCGCA CTGCTCTGGA AATCAAGCTC GCCTCAGGGG GTGTTAAGCA GCACCAGTGT 
GATGCTGAGC TGAGAAAGGA GATATCTCTA GTTTGGCCCA ACCTGTCCCA GAAGACACTG 
GATTTGCTGG TGCCTCCTCA CAAACCTGAT GAGATGACTG TGGGGAAGGT CTACGCAGCG 
CTCATGATAT TTGACTTCTA CAAACAGAAC AAGAACAGCA GGGAACAAGT CCACCAGCCT 
CCTGGGGGCC TGTGCCAGCC CGGACCAGTG TCACTCTTCC ACCCCTTGAA GGCCACTTTG 
GAGCAAACCC AGCCCTCGGC ATTCAGCAGT GCGAAGGCAT TCCTGCGCCA GAAAAGCTCG 
GCCTCACTCA ACAATGGAGG CACATTGCCA GCCCCAGAGG GTGGGATCAA AGAGTCCAGT 
TCGTGGGGGA CCCAACGGAC CCAGGACGTG TTTTATGAAA CCAGGACACC AGCTTTTGAA 
CGAGGCCACT CAGAGGAAAT CCCCATTGAA CGGGTGGTGG AAATGAGGGA AATCAGCCCC 
ACACTTGCCA ATGGAGAGCA CCAACCAGGG CTGGAGAGCC AGGGTCGGGC GGCTTCCATG 
CCGCGCCTGG CTGCTGAAAC GCAGCCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGGGCAGT GGTTCGGTTA ACGGGAGCCC CTTGCTGTCG 
ACATCTGGTG CTAGTACCCC CTGCCGGGGT CGGAGGCAGC TCCCGCAGAC CCCCCTGACC 
CCACGCCCCA GCATCACTTA CAAGACCGCC AACTCCTCGC CCGTACACTT CAGCACGTCG 
CCGGGCGGCC TCCCGCCCTT CTCCCCGGGC CGCCTGAGCC GCGGGCTGTC TGAGCACAAT 
GCACTGCTGC GTGGGGACCA GCAGCCGCCC CCGGCCGCGG TTGCCCGCAT CGGCTCCGAC 
CCTTACCTGG GCCACCGCGA CGCCGCCGAC AGCCCCATAG GCGCAGCGCC CGAGGACACG 
CTCACCTTCG AGGAAGCCGT GGCCACCAAC TCGGGGCGTT CCTCCAGGAC TTCTTACGTC 
TCCTCCTTGA CCTCCCAGTC TCACCAAGCA CGCCGGGTGC CCAATGGCTA CCATTACACG 
CTGGGGCTCA ACACGGGGCC CGGCACTGGC ACCAGGGGAC GGAGCTACTA CCACGAAGCC 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279265.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134751.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X6, mRNA.

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CloneID	OGa43291
Clone ID Related Accession (Same CDS sequence)	XM_015279265.1
Accession Version	XM_015279265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACAACTTC 
ATCAACCTCA GCTTCCTGCG GCTCTTCAGG GCAGCAAGAC TTATCAAGCT CCTTCGCCAG 
GGTTACACTA TCCGCATCTT GCTCTGGACG TTTGTGCAGT CATTTAAGGC CTTGCCTTAT 
GTGTGTCTTC TCATTGCAAT GCTCTTCTTC ATCTATGCCA TTATTGGCAT GCAGGTATTT 
GGAAACATTG CACTAAATGA CGAAACCTCC ATCAATCGGC ACAATAATTT CCGTACCTTC 
CTTCAAGCCT TGATGCTTCT GTTCAGGAGT GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCATGCC TGAGCAACAG GGCCTGCGAC CCACTCTCCG GCCTCACCAA GAACGAGTGC 
GGCAGCGAGT TCGCCTACTT CTACTTCGTG TCATTCATCT TCCTCTGCTC CTTCCTGATG 
TTGAACCTAT TTGTGGCTGT AATCATGGAC AACTTTGAAT ACTTGACAAG AGACTCCTCC 
ATCCTTGGGC CCCATCATTT GGATGAGTTT GTCCGTGTCT GGGCTGAATA CGACCCAGCT 
GCCTGCGGGC GCATCAGTTA CACAGACATG TATGAGATGC TGAGACACAT GTCCCCACCT 
CTAGGCCTTG GAAAGAAATG CCCTGCTCGC GTTGCCTATA AGCGCCTGGT GCGGATGAAC 
ATGCCCATCT CACCCGAGGA TCTGACAGTC CACTTCACCT CCACGCTGAT GGCCCTGATC 
CGCACTGCTC TGGAAATCAA GCTCGCCTCA GGGGGTGTTA AGCAGCACCA GTGTGATGCT 
GAGCTGAGAA AGGAGATATC TCTAGTTTGG CCCAACCTGT CCCAGAAGAC ACTGGATTTG 
CTGGTGCCTC CTCACAAACC TGATGAGATG ACTGTGGGGA AGGTCTACGC AGCGCTCATG 
ATATTTGACT TCTACAAACA GAACAAGAAC AGCAGGGAAC AAGTCCACCA GCCTCCTGGG 
GGCCTGTGCC AGCCCGGACC AGTGTCACTC TTCCACCCCT TGAAGGCCAC TTTGGAGCAA 
ACCCAGCCCT CGGCATTCAG CAGTGCGAAG GCATTCCTGC GCCAGAAAAG CTCGGCCTCA 
CTCAACAATG GAGGCACATT GCCAGCCCCA GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG 
GGGACCCAAC GGACCCAGGA CGTGTTTTAT GAAACCAGGA CACCAGCTTT TGAACGAGGC 
CACTCAGAGG AAATCCCCAT TGAACGGGTG GTGGAAATGA GGGAAATCAG CCCCACACTT 
GCCAATGGAG AGCACCAACC AGGGCTGGAG AGCCAGGGTC GGGCGGCTTC CATGCCGCGC 
CTGGCTGCTG AAACGCAGCC CATCCCGGAC ACAAGCCCCA TGAAGCGCTC CATCTCCACG 
CTGACCCCGC AGCGTCCTCA CCCCATGCAC CTCTATGAGT ACTCCCTGGA GCGCGTGCCC 
ACGGACCAGG TGCATCACCA CCACCACCAC CGCTGCCACC GGCGGAAAGA GAAGAAGCAG 
AAGTCCTTGG ACAGGACCAC ACATCAGCTG GCCGATGGGG AAGCTGTGGC CCAGTCTGGT 
GAATCTTCTT CCAAGGACAA GAAGCAAGAG CGTGGACGGT CCCAAGAGAG GAAGCAGCAC 
TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC TACTCCTGTG ATCGCTATGG CAGCCGGGAC 
CGATCCCAGC CCAAGTCTGC TGATCAGAGC AGGCCTACTT CACCCAATGG TGGGCCTGAG 
CAAGGTCCCC ACAGACAGGG CAGTGGTTCG GTTAACGGGA GCCCCTTGCT GTCGACATCT 
GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG CAGCTCCCGC AGACCCCCCT GACCCCACGC 
CCCAGCATCA CTTACAAGAC CGCCAACTCC TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC 
GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG AGCCGCGGGC TGTCTGAGCA CAATGCACTG 
CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC GCGGTTGCCC GCATCGGCTC CGACCCTTAC 
CTGGGCCACC GCGACGCCGC CGACAGCCCC ATAGGCGCAG CGCCCGAGGA CACGCTCACC 
TTCGAGGAAG CCGTGGCCAC CAACTCGGGG CGTTCCTCCA GGACTTCTTA CGTCTCCTCC 
TTGACCTCCC AGTCTCACCA AGCACGCCGG GTGCCCAATG GCTACCATTA CACGCTGGGG 
CTCAACACGG GGCCCGGCAC TGGCACCAGG GGACGGAGCT ACTACCACGA AGCCGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134751.1
CDS	425..7519
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279265.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279265.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACAACTTC 
ATCAACCTCA GCTTCCTGCG GCTCTTCAGG GCAGCAAGAC TTATCAAGCT CCTTCGCCAG 
GGTTACACTA TCCGCATCTT GCTCTGGACG TTTGTGCAGT CATTTAAGGC CTTGCCTTAT 
GTGTGTCTTC TCATTGCAAT GCTCTTCTTC ATCTATGCCA TTATTGGCAT GCAGGTATTT 
GGAAACATTG CACTAAATGA CGAAACCTCC ATCAATCGGC ACAATAATTT CCGTACCTTC 
CTTCAAGCCT TGATGCTTCT GTTCAGGAGT GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCATGCC TGAGCAACAG GGCCTGCGAC CCACTCTCCG GCCTCACCAA GAACGAGTGC 
GGCAGCGAGT TCGCCTACTT CTACTTCGTG TCATTCATCT TCCTCTGCTC CTTCCTGATG 
TTGAACCTAT TTGTGGCTGT AATCATGGAC AACTTTGAAT ACTTGACAAG AGACTCCTCC 
ATCCTTGGGC CCCATCATTT GGATGAGTTT GTCCGTGTCT GGGCTGAATA CGACCCAGCT 
GCCTGCGGGC GCATCAGTTA CACAGACATG TATGAGATGC TGAGACACAT GTCCCCACCT 
CTAGGCCTTG GAAAGAAATG CCCTGCTCGC GTTGCCTATA AGCGCCTGGT GCGGATGAAC 
ATGCCCATCT CACCCGAGGA TCTGACAGTC CACTTCACCT CCACGCTGAT GGCCCTGATC 
CGCACTGCTC TGGAAATCAA GCTCGCCTCA GGGGGTGTTA AGCAGCACCA GTGTGATGCT 
GAGCTGAGAA AGGAGATATC TCTAGTTTGG CCCAACCTGT CCCAGAAGAC ACTGGATTTG 
CTGGTGCCTC CTCACAAACC TGATGAGATG ACTGTGGGGA AGGTCTACGC AGCGCTCATG 
ATATTTGACT TCTACAAACA GAACAAGAAC AGCAGGGAAC AAGTCCACCA GCCTCCTGGG 
GGCCTGTGCC AGCCCGGACC AGTGTCACTC TTCCACCCCT TGAAGGCCAC TTTGGAGCAA 
ACCCAGCCCT CGGCATTCAG CAGTGCGAAG GCATTCCTGC GCCAGAAAAG CTCGGCCTCA 
CTCAACAATG GAGGCACATT GCCAGCCCCA GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG 
GGGACCCAAC GGACCCAGGA CGTGTTTTAT GAAACCAGGA CACCAGCTTT TGAACGAGGC 
CACTCAGAGG AAATCCCCAT TGAACGGGTG GTGGAAATGA GGGAAATCAG CCCCACACTT 
GCCAATGGAG AGCACCAACC AGGGCTGGAG AGCCAGGGTC GGGCGGCTTC CATGCCGCGC 
CTGGCTGCTG AAACGCAGCC CATCCCGGAC ACAAGCCCCA TGAAGCGCTC CATCTCCACG 
CTGACCCCGC AGCGTCCTCA CCCCATGCAC CTCTATGAGT ACTCCCTGGA GCGCGTGCCC 
ACGGACCAGG TGCATCACCA CCACCACCAC CGCTGCCACC GGCGGAAAGA GAAGAAGCAG 
AAGTCCTTGG ACAGGACCAC ACATCAGCTG GCCGATGGGG AAGCTGTGGC CCAGTCTGGT 
GAATCTTCTT CCAAGGACAA GAAGCAAGAG CGTGGACGGT CCCAAGAGAG GAAGCAGCAC 
TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC TACTCCTGTG ATCGCTATGG CAGCCGGGAC 
CGATCCCAGC CCAAGTCTGC TGATCAGAGC AGGCCTACTT CACCCAATGG TGGGCCTGAG 
CAAGGTCCCC ACAGACAGGG CAGTGGTTCG GTTAACGGGA GCCCCTTGCT GTCGACATCT 
GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG CAGCTCCCGC AGACCCCCCT GACCCCACGC 
CCCAGCATCA CTTACAAGAC CGCCAACTCC TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC 
GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG AGCCGCGGGC TGTCTGAGCA CAATGCACTG 
CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC GCGGTTGCCC GCATCGGCTC CGACCCTTAC 
CTGGGCCACC GCGACGCCGC CGACAGCCCC ATAGGCGCAG CGCCCGAGGA CACGCTCACC 
TTCGAGGAAG CCGTGGCCAC CAACTCGGGG CGTTCCTCCA GGACTTCTTA CGTCTCCTCC 
TTGACCTCCC AGTCTCACCA AGCACGCCGG GTGCCCAATG GCTACCATTA CACGCTGGGG 
CTCAACACGG GGCCCGGCAC TGGCACCAGG GGACGGAGCT ACTACCACGA AGCCGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279266.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134752.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X7, mRNA.

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CloneID	OGa43292
Clone ID Related Accession (Same CDS sequence)	XM_015279266.1
Accession Version	XM_015279266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACAACTTC 
ATCAACCTCA GCTTCCTGCG GCTCTTCAGG GCAGCAAGAC TTATCAAGCT CCTTCGCCAG 
GGTTACACTA TCCGCATCTT GCTCTGGACG TTTGTGCAGT CATTTAAGGC CTTGCCTTAT 
GTGTGTCTTC TCATTGCAAT GCTCTTCTTC ATCTATGCCA TTATTGGCAT GCAGGTATTT 
GGAAACATTG CACTAAATGA CGAAACCTCC ATCAATCGGC ACAATAATTT CCGTACCTTC 
CTTCAAGCCT TGATGCTTCT GTTCAGGAGT GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCATGCC TGAGCAACAG GGCCTGCGAC CCACTCTCCG GCCTCACCAA GAACGAGTGC 
GGCAGCGAGT TCGCCTACTT CTACTTCGTG TCATTCATCT TCCTCTGCTC CTTCCTGATG 
TTGAACCTAT TTGTGGCTGT AATCATGGAC AACTTTGAAT ACTTGACAAG AGACTCCTCC 
ATCCTTGGGC CCCATCATTT GGATGAGTTT GTCCGTGTCT GGGCTGAATA CGACCCAGCT 
GCCTGTTGCC GGATTCATTA TAAGGATATG TACAATTTGT TACGTGTAAT TGCTCCACCC 
CTGGGCTTAG GAAAGAAGTG CCCTCATAGG GTGGCATACA AGCGCCTGGT GCGGATGAAC 
ATGCCCATCT CACCCGAGGA TCTGACAGTC CACTTCACCT CCACGCTGAT GGCCCTGATC 
CGCACTGCTC TGGAAATCAA GCTCGCCTCA GGGGGTGTTA AGCAGCACCA GTGTGATGCT 
GAGCTGAGAA AGGAGATATC TCTAGTTTGG CCCAACCTGT CCCAGAAGAC ACTGGATTTG 
CTGGTGCCTC CTCACAAACC TGATGAGATG ACTGTGGGGA AGGTCTACGC AGCGCTCATG 
ATATTTGACT TCTACAAACA GAACAAGAAC AGCAGGGAAC AAGTCCACCA GCCTCCTGGG 
GGCCTGTGCC AGCCCGGACC AGTGTCACTC TTCCACCCCT TGAAGGCCAC TTTGGAGCAA 
ACCCAGCCCT CGGCATTCAG CAGTGCGAAG GCATTCCTGC GCCAGAAAAG CTCGGCCTCA 
CTCAACAATG GAGGCACATT GCCAGCCCCA GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG 
GGGACCCAAC GGACCCAGGA CGTGTTTTAT GAAACCAGGA CACCAGCTTT TGAACGAGGC 
CACTCAGAGG AAATCCCCAT TGAACGGGTG GTGGAAATGA GGGAAATCAG CCCCACACTT 
GCCAATGGAG AGCACCAACC AGGGCTGGAG AGCCAGGGTC GGGCGGCTTC CATGCCGCGC 
CTGGCTGCTG AAACGCAGCC CATCCCGGAC ACAAGCCCCA TGAAGCGCTC CATCTCCACG 
CTGACCCCGC AGCGTCCTCA CCCCATGCAC CTCTATGAGT ACTCCCTGGA GCGCGTGCCC 
ACGGACCAGG TGCATCACCA CCACCACCAC CGCTGCCACC GGCGGAAAGA GAAGAAGCAG 
AAGTCCTTGG ACAGGACCAC ACATCAGCTG GCCGATGGGG AAGCTGTGGC CCAGTCTGGT 
GAATCTTCTT CCAAGGACAA GAAGCAAGAG CGTGGACGGT CCCAAGAGAG GAAGCAGCAC 
TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC TACTCCTGTG ATCGCTATGG CAGCCGGGAC 
CGATCCCAGC CCAAGTCTGC TGATCAGAGC AGGCCTACTT CACCCAATGG TGGGCCTGAG 
CAAGGTCCCC ACAGACAGGG CAGTGGTTCG GTTAACGGGA GCCCCTTGCT GTCGACATCT 
GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG CAGCTCCCGC AGACCCCCCT GACCCCACGC 
CCCAGCATCA CTTACAAGAC CGCCAACTCC TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC 
GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG AGCCGCGGGC TGTCTGAGCA CAATGCACTG 
CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC GCGGTTGCCC GCATCGGCTC CGACCCTTAC 
CTGGGCCACC GCGACGCCGC CGACAGCCCC ATAGGCGCAG CGCCCGAGGA CACGCTCACC 
TTCGAGGAAG CCGTGGCCAC CAACTCGGGG CGTTCCTCCA GGACTTCTTA CGTCTCCTCC 
TTGACCTCCC AGTCTCACCA AGCACGCCGG GTGCCCAATG GCTACCATTA CACGCTGGGG 
CTCAACACGG GGCCCGGCAC TGGCACCAGG GGACGGAGCT ACTACCACGA AGCCGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134752.1
CDS	427..7521
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279266.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279266.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCAGGAACC 
AAAGGCAAGG ACATCAACAC GATCAAGTCG CTGCGGGTTC TGCGGGTCCT ACGACCACTG 
AAGACCATTA AACGGCTGCC AAAACTAAAG GCTGTCTTTG ACTGCGTGGT GAATTCCCTG 
AAGAACGTCC TCAACATCCT CATTGTCTAC ATGCTCTTCA TGTTCATCTT TGCTGTCATT 
GCTGTGCAGC TCTTCAAAGG CAGATTCTTC TACTGCACTG ATGAGTCGAA AGAGCTGGAG 
AAGGATTGCA GGGGACAATA CCTTGACTAT GAAAAAAATG AGGTGGAGGC GCAACCCAGG 
GAATGGAAAA AATATGAGTT CCATTACGAC AATGTGCTGT GGGCTCTGCT GACACTTTTC 
ACTGTCTCCA CAGGAGAAGG GTGGCCAACT GTGCTGAAGC ACTCAGTGGA TGCTACCTAC 
GAGGAACAGG GTCCCAGCCC TGGCTACCGA ATGGAAATGT CTATCTTTTA CGTGGTGTAT 
TTTGTTGTCT TCCCTTTTTT CTTTGTGAAC ATCTTTGTGG CGTTAATCAT AATCACCTTC 
CAAGAGCAAG GAGATAAAGT GATGTCAGAA TGCAGCCTAG AGAAAAATGA GAGGGCCTGC 
ATTGACTTCG CCATAAGTGC CAAGCCACTG ACCCGCTACA TGCCTCAGAA CAAGCAGTCA 
TTTCAGTACA AGATGTGGAA ATTTGTGGTG TCCCCGCCCT TTGAGTATTT TATCATGGTC 
ATGATTGCAC TCAATACTAT TGTTCTCATG ATGAAGTTCT ATGATGCTCC AGAAGCATAT 
GAAGAAATGT TGAAATGCCT GAATATTGTC TTTACATCCA TGTTTTCAAT GGAGTGTGTG 
CTGAAGATCA TTGCCTTTGG TGTGCTGAAT TATTTCCGAG ATGCCTGGAA TGTCTTTGAT 
TTTGTAACAG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGACAACTTC 
ATCAACCTCA GCTTCCTGCG GCTCTTCAGG GCAGCAAGAC TTATCAAGCT CCTTCGCCAG 
GGTTACACTA TCCGCATCTT GCTCTGGACG TTTGTGCAGT CATTTAAGGC CTTGCCTTAT 
GTGTGTCTTC TCATTGCAAT GCTCTTCTTC ATCTATGCCA TTATTGGCAT GCAGGTATTT 
GGAAACATTG CACTAAATGA CGAAACCTCC ATCAATCGGC ACAATAATTT CCGTACCTTC 
CTTCAAGCCT TGATGCTTCT GTTCAGGAGT GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCATGCC TGAGCAACAG GGCCTGCGAC CCACTCTCCG GCCTCACCAA GAACGAGTGC 
GGCAGCGAGT TCGCCTACTT CTACTTCGTG TCATTCATCT TCCTCTGCTC CTTCCTGATG 
TTGAACCTAT TTGTGGCTGT AATCATGGAC AACTTTGAAT ACTTGACAAG AGACTCCTCC 
ATCCTTGGGC CCCATCATTT GGATGAGTTT GTCCGTGTCT GGGCTGAATA CGACCCAGCT 
GCCTGTTGCC GGATTCATTA TAAGGATATG TACAATTTGT TACGTGTAAT TGCTCCACCC 
CTGGGCTTAG GAAAGAAGTG CCCTCATAGG GTGGCATACA AGCGCCTGGT GCGGATGAAC 
ATGCCCATCT CACCCGAGGA TCTGACAGTC CACTTCACCT CCACGCTGAT GGCCCTGATC 
CGCACTGCTC TGGAAATCAA GCTCGCCTCA GGGGGTGTTA AGCAGCACCA GTGTGATGCT 
GAGCTGAGAA AGGAGATATC TCTAGTTTGG CCCAACCTGT CCCAGAAGAC ACTGGATTTG 
CTGGTGCCTC CTCACAAACC TGATGAGATG ACTGTGGGGA AGGTCTACGC AGCGCTCATG 
ATATTTGACT TCTACAAACA GAACAAGAAC AGCAGGGAAC AAGTCCACCA GCCTCCTGGG 
GGCCTGTGCC AGCCCGGACC AGTGTCACTC TTCCACCCCT TGAAGGCCAC TTTGGAGCAA 
ACCCAGCCCT CGGCATTCAG CAGTGCGAAG GCATTCCTGC GCCAGAAAAG CTCGGCCTCA 
CTCAACAATG GAGGCACATT GCCAGCCCCA GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG 
GGGACCCAAC GGACCCAGGA CGTGTTTTAT GAAACCAGGA CACCAGCTTT TGAACGAGGC 
CACTCAGAGG AAATCCCCAT TGAACGGGTG GTGGAAATGA GGGAAATCAG CCCCACACTT 
GCCAATGGAG AGCACCAACC AGGGCTGGAG AGCCAGGGTC GGGCGGCTTC CATGCCGCGC 
CTGGCTGCTG AAACGCAGCC CATCCCGGAC ACAAGCCCCA TGAAGCGCTC CATCTCCACG 
CTGACCCCGC AGCGTCCTCA CCCCATGCAC CTCTATGAGT ACTCCCTGGA GCGCGTGCCC 
ACGGACCAGG TGCATCACCA CCACCACCAC CGCTGCCACC GGCGGAAAGA GAAGAAGCAG 
AAGTCCTTGG ACAGGACCAC ACATCAGCTG GCCGATGGGG AAGCTGTGGC CCAGTCTGGT 
GAATCTTCTT CCAAGGACAA GAAGCAAGAG CGTGGACGGT CCCAAGAGAG GAAGCAGCAC 
TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC TACTCCTGTG ATCGCTATGG CAGCCGGGAC 
CGATCCCAGC CCAAGTCTGC TGATCAGAGC AGGCCTACTT CACCCAATGG TGGGCCTGAG 
CAAGGTCCCC ACAGACAGGG CAGTGGTTCG GTTAACGGGA GCCCCTTGCT GTCGACATCT 
GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG CAGCTCCCGC AGACCCCCCT GACCCCACGC 
CCCAGCATCA CTTACAAGAC CGCCAACTCC TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC 
GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG AGCCGCGGGC TGTCTGAGCA CAATGCACTG 
CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC GCGGTTGCCC GCATCGGCTC CGACCCTTAC 
CTGGGCCACC GCGACGCCGC CGACAGCCCC ATAGGCGCAG CGCCCGAGGA CACGCTCACC 
TTCGAGGAAG CCGTGGCCAC CAACTCGGGG CGTTCCTCCA GGACTTCTTA CGTCTCCTCC 
TTGACCTCCC AGTCTCACCA AGCACGCCGG GTGCCCAATG GCTACCATTA CACGCTGGGG 
CTCAACACGG GGCCCGGCAC TGGCACCAGG GGACGGAGCT ACTACCACGA AGCCGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279267.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134753.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X8, mRNA.

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CloneID	OGa43293
Clone ID Related Accession (Same CDS sequence)	XM_015279267.1
Accession Version	XM_015279267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCTTTTGTAA AGCAAACTCG AGGTACTGTA TCTCGCAGCT CGTCAGTCTC CAGCGTAAAT 
TCACCCAAGC AGCAGAACTC CTCCAAATCC AAGTCGGTTT GGGAGCAACG AACCAGCCAG 
ATCCGGATGC ACAACTTCCG AGCCAGCTGC GAGGCTCTGT ACAACGAACT GGACCCCGAG 
GAGCGGGTGC GCTACGCCAC GACCCTACAC ATCCGTCCAG ACATGAAGAC CCACTTGGAT 
CGGCCACTGG TGGTAGAGCC AAGGGGTGAA GGGAGGAACA ACATCAGTAA GCTGAGCCCT 
GTGGATGTGC AGGAGGTGGA GCAGACCAAA GTCAGCTCTA CCGACGGAGC AGAAGCTCCG 
CGGAAGCATC ACCGGCATCG GGATAAGGAG AAACTGGGAG AGCAAGAGAA GGGTGATGTG 
ACCAAAGATG AGAATGGGGA ATCTGGCATC AACAATAAGG AGGAGAGGCA CAGGCAGCAC 
AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG 
GGCCAGGAAG GAGGGAAGAG GCACCATCGC CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG 
GAGCACCGGA GGCACCGCAC GCACCGGCAC TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT 
GGGACCATCA ACGGGGCCAG GAGTGAGCGG CGGACCCGCC ACCGTGGAGG GTCGAGGTCT 
GGGAACCGGG AAGGAGAGCC TGGGTCCAAG GGCGAGAATG GAGAGGAGCC TCACAGACGG 
CACCGGTTCA GGAGCAGGGC GCTGTCGACG TACGATTCGG TGGAGAAGGA GAACAGGGAG 
AAGGAAGGGG AGACTGCTGA GAAGGAGCAC CAGAATCATC AGCCCAAGGA GAATCAGTGT 
GAGATTGAGG CCAGTGGGAG TGTGAGCATC CCGGTGCACA CCCTTCCCAG CACCTATCTG 
CAGAAAGTGC CAGAGCAGCC TGAAGATGCT GACAACCAGA AGAACGTGAC ACGCATGATT 
CAGCCGCCTC TGGACAAAAC CACCACTGTG AACATCCCCG TCACCATCAC CGCCCCGCCA 
GGAGAAACCA CCGTCATACC TATGAACAAC GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG 
AAGGACGTTG ATGACTTGAC GAAAAATGGG CCTAAACCAA TCTTGCCTTA CAGTTCCATG 
TTCATCCTAA GTCCTACGAA CCCGATCCGG CGTCTGTTCC ACTACATCGT CAACCTGCGC 
TACTTCGAGA TGGTCATCCT CATCGTCATC GCTCTCAGCA GCATTGCCCT GGCAGCAGAA 
GACCCCGTCC AAGCAGAATC ACCAAGGAAC GATGCTCTGA AGTACTTGGA TTATATATTC 
ACGGGTGTCT TTACCTTCGA GATGGTGATC AAGATGATTG ACCTGGGGCT GTTACTTCAC 
CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC ATCTTGGACT TCATTGTGGT CAGTGGGGCC 
TTGGTGGCAT TTGCTTTCTC GAGTTTCATG GGAGGAACCA AAGGCAAGGA CATCAACACG 
ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA CGACCACTGA AGACCATTAA ACGGCTGCCA 
AAACTAAAGG CTGTCTTTGA CTGCGTGGTG AATTCCCTGA AGAACGTCCT CAACATCCTC 
ATTGTCTACA TGCTCTTCAT GTTCATCTTT GCTGTCATTG CTGTGCAGCT CTTCAAAGGC 
AGATTCTTCT ACTGCACTGA TGAGTCGAAA GAGCTGGAGA AGGATTGCAG GGGACAATAC 
CTTGACTATG AAAAAAATGA GGTGGAGGCG CAACCCAGGG AATGGAAAAA ATATGAGTTC 
CATTACGACA ATGTGCTGTG GGCTCTGCTG ACACTTTTCA CTGTCTCCAC AGGAGAAGGG 
TGGCCAACTG TGCTGAAGCA CTCAGTGGAT GCTACCTACG AGGAACAGGG TCCCAGCCCT 
GGCTACCGAA TGGAAATGTC TATCTTTTAC GTGGTGTATT TTGTTGTCTT CCCTTTTTTC 
TTTGTGAACA TCTTTGTGGC GTTAATCATA ATCACCTTCC AAGAGCAAGG AGATAAAGTG 
ATGTCAGAAT GCAGCCTAGA GAAAAATGAG AGGGCCTGCA TTGACTTCGC CATAAGTGCC 
AAGCCACTGA CCCGCTACAT GCCTCAGAAC AAGCAGTCAT TTCAGTACAA GATGTGGAAA 
TTTGTGGTGT CCCCGCCCTT TGAGTATTTT ATCATGGTCA TGATTGCACT CAATACTATT 
GTTCTCATGA TGAAGTTCTA TGATGCTCCA GAAGCATATG AAGAAATGTT GAAATGCCTG 
AATATTGTCT TTACATCCAT GTTTTCAATG GAGTGTGTGC TGAAGATCAT TGCCTTTGGT 
GTGCTGAATT ATTTCCGAGA TGCCTGGAAT GTCTTTGATT TTGTAACAGT GTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATTGCG GACACGGACA ACTTCATCAA CCTCAGCTTC 
CTGCGGCTCT TCAGGGCAGC AAGACTTATC AAGCTCCTTC GCCAGGGTTA CACTATCCGC 
ATCTTGCTCT GGACGTTTGT GCAGTCATTT AAGGCCTTGC CTTATGTGTG TCTTCTCATT 
GCAATGCTCT TCTTCATCTA TGCCATTATT GGCATGCAGG TATTTGGAAA CATTGCACTA 
AATGACGAAA CCTCCATCAA TCGGCACAAT AATTTCCGTA CCTTCCTTCA AGCCTTGATG 
CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC TGGCACGAGA TCATGCTGTC ATGCCTGAGC 
AACAGGGCCT GCGACCCACT CTCCGGCCTC ACCAAGAACG AGTGCGGCAG CGAGTTCGCC 
TACTTCTACT TCGTGTCATT CATCTTCCTC TGCTCCTTCC TGATGTTGAA CCTATTTGTG 
GCTGTAATCA TGGACAACTT TGAATACTTG ACAAGAGACT CCTCCATCCT TGGGCCCCAT 
CATTTGGATG AGTTTGTCCG TGTCTGGGCT GAATACGACC CAGCTGCCTG CGGGCGCATC 
AGTTACACAG ACATGTATGA GATGCTGAGA CACATGTCCC CACCTCTAGG CCTTGGAAAG 
AAATGCCCTG CTCGCGTTGC CTATAAGCGC CTGGTGCGGA TGAACATGCC CATCTCACCC 
GAGGATCTGA CAGTCCACTT CACCTCCACG CTGATGGCCC TGATCCGCAC TGCTCTGGAA 
ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG CACCAGTGTG ATGCTGAGCT GAGAAAGGAG 
ATATCTCTAG TTTGGCCCAA CCTGTCCCAG AAGACACTGG ATTTGCTGGT GCCTCCTCAC 
AAACCTGATG AGATGACTGT GGGGAAGGTC TACGCAGCGC TCATGATATT TGACTTCTAC 
AAACAGAACA AGAACAGCAG GGAACAAGTC CACCAGCCTC CTGGGGGCCT GTGCCAGCCC 
GGACCAGTGT CACTCTTCCA CCCCTTGAAG GCCACTTTGG AGCAAACCCA GCCCTCGGCA 
TTCAGCAGTG CGAAGGCATT CCTGCGCCAG AAAAGCTCGG CCTCACTCAA CAATGGAGGC 
ACATTGCCAG CCCCAGAGGG TGGGATCAAA GAGTCCAGTT CGTGGGGGAC CCAACGGACC 
CAGGACGTGT TTTATGAAAC CAGGACACCA GCTTTTGAAC GAGGCCACTC AGAGGAAATC 
CCCATTGAAC GGGTGGTGGA AATGAGGGAA ATCAGCCCCA CACTTGCCAA TGGAGAGCAC 
CAACCAGGGC TGGAGAGCCA GGGTCGGGCG GCTTCCATGC CGCGCCTGGC TGCTGAAACG 
CAGCCCATCC CGGACACAAG CCCCATGAAG CGCTCCATCT CCACGCTGAC CCCGCAGCGT 
CCTCACCCCA TGCACCTCTA TGAGTACTCC CTGGAGCGCG TGCCCACGGA CCAGGTGCAT 
CACCACCACC ACCACCGCTG CCACCGGCGG AAAGAGAAGA AGCAGAAGTC CTTGGACAGG 
ACCACACATC AGCTGGCCGA TGGGGAAGCT GTGGCCCAGT CTGGTGAATC TTCTTCCAAG 
GACAAGAAGC AAGAGCGTGG ACGGTCCCAA GAGAGGAAGC AGCACTCTTC CTCCTCCTCA 
GAAAAGCAGC GCTTCTACTC CTGTGATCGC TATGGCAGCC GGGACCGATC CCAGCCCAAG 
TCTGCTGATC AGAGCAGGCC TACTTCACCC AATGGTGGGC CTGAGCAAGG TCCCCACAGA 
CAGGGCAGTG GTTCGGTTAA CGGGAGCCCC TTGCTGTCGA CATCTGGTGC TAGTACCCCC 
TGCCGGGGTC GGAGGCAGCT CCCGCAGACC CCCCTGACCC CACGCCCCAG CATCACTTAC 
AAGACCGCCA ACTCCTCGCC CGTACACTTC AGCACGTCGC CGGGCGGCCT CCCGCCCTTC 
TCCCCGGGCC GCCTGAGCCG CGGGCTGTCT GAGCACAATG CACTGCTGCG TGGGGACCAG 
CAGCCGCCCC CGGCCGCGGT TGCCCGCATC GGCTCCGACC CTTACCTGGG CCACCGCGAC 
GCCGCCGACA GCCCCATAGG CGCAGCGCCC GAGGACACGC TCACCTTCGA GGAAGCCGTG 
GCCACCAACT CGGGGCGTTC CTCCAGGACT TCTTACGTCT CCTCCTTGAC CTCCCAGTCT 
CACCAAGCAC GCCGGGTGCC CAATGGCTAC CATTACACGC TGGGGCTCAA CACGGGGCCC 
GGCACTGGCA CCAGGGGACG GAGCTACTAC CACGAAGCCG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134753.1
CDS	425..7504
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279267.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279267.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCTTTTGTAA AGCAAACTCG AGGTACTGTA TCTCGCAGCT CGTCAGTCTC CAGCGTAAAT 
TCACCCAAGC AGCAGAACTC CTCCAAATCC AAGTCGGTTT GGGAGCAACG AACCAGCCAG 
ATCCGGATGC ACAACTTCCG AGCCAGCTGC GAGGCTCTGT ACAACGAACT GGACCCCGAG 
GAGCGGGTGC GCTACGCCAC GACCCTACAC ATCCGTCCAG ACATGAAGAC CCACTTGGAT 
CGGCCACTGG TGGTAGAGCC AAGGGGTGAA GGGAGGAACA ACATCAGTAA GCTGAGCCCT 
GTGGATGTGC AGGAGGTGGA GCAGACCAAA GTCAGCTCTA CCGACGGAGC AGAAGCTCCG 
CGGAAGCATC ACCGGCATCG GGATAAGGAG AAACTGGGAG AGCAAGAGAA GGGTGATGTG 
ACCAAAGATG AGAATGGGGA ATCTGGCATC AACAATAAGG AGGAGAGGCA CAGGCAGCAC 
AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG 
GGCCAGGAAG GAGGGAAGAG GCACCATCGC CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG 
GAGCACCGGA GGCACCGCAC GCACCGGCAC TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT 
GGGACCATCA ACGGGGCCAG GAGTGAGCGG CGGACCCGCC ACCGTGGAGG GTCGAGGTCT 
GGGAACCGGG AAGGAGAGCC TGGGTCCAAG GGCGAGAATG GAGAGGAGCC TCACAGACGG 
CACCGGTTCA GGAGCAGGGC GCTGTCGACG TACGATTCGG TGGAGAAGGA GAACAGGGAG 
AAGGAAGGGG AGACTGCTGA GAAGGAGCAC CAGAATCATC AGCCCAAGGA GAATCAGTGT 
GAGATTGAGG CCAGTGGGAG TGTGAGCATC CCGGTGCACA CCCTTCCCAG CACCTATCTG 
CAGAAAGTGC CAGAGCAGCC TGAAGATGCT GACAACCAGA AGAACGTGAC ACGCATGATT 
CAGCCGCCTC TGGACAAAAC CACCACTGTG AACATCCCCG TCACCATCAC CGCCCCGCCA 
GGAGAAACCA CCGTCATACC TATGAACAAC GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG 
AAGGACGTTG ATGACTTGAC GAAAAATGGG CCTAAACCAA TCTTGCCTTA CAGTTCCATG 
TTCATCCTAA GTCCTACGAA CCCGATCCGG CGTCTGTTCC ACTACATCGT CAACCTGCGC 
TACTTCGAGA TGGTCATCCT CATCGTCATC GCTCTCAGCA GCATTGCCCT GGCAGCAGAA 
GACCCCGTCC AAGCAGAATC ACCAAGGAAC GATGCTCTGA AGTACTTGGA TTATATATTC 
ACGGGTGTCT TTACCTTCGA GATGGTGATC AAGATGATTG ACCTGGGGCT GTTACTTCAC 
CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC ATCTTGGACT TCATTGTGGT CAGTGGGGCC 
TTGGTGGCAT TTGCTTTCTC GAGTTTCATG GGAGGAACCA AAGGCAAGGA CATCAACACG 
ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA CGACCACTGA AGACCATTAA ACGGCTGCCA 
AAACTAAAGG CTGTCTTTGA CTGCGTGGTG AATTCCCTGA AGAACGTCCT CAACATCCTC 
ATTGTCTACA TGCTCTTCAT GTTCATCTTT GCTGTCATTG CTGTGCAGCT CTTCAAAGGC 
AGATTCTTCT ACTGCACTGA TGAGTCGAAA GAGCTGGAGA AGGATTGCAG GGGACAATAC 
CTTGACTATG AAAAAAATGA GGTGGAGGCG CAACCCAGGG AATGGAAAAA ATATGAGTTC 
CATTACGACA ATGTGCTGTG GGCTCTGCTG ACACTTTTCA CTGTCTCCAC AGGAGAAGGG 
TGGCCAACTG TGCTGAAGCA CTCAGTGGAT GCTACCTACG AGGAACAGGG TCCCAGCCCT 
GGCTACCGAA TGGAAATGTC TATCTTTTAC GTGGTGTATT TTGTTGTCTT CCCTTTTTTC 
TTTGTGAACA TCTTTGTGGC GTTAATCATA ATCACCTTCC AAGAGCAAGG AGATAAAGTG 
ATGTCAGAAT GCAGCCTAGA GAAAAATGAG AGGGCCTGCA TTGACTTCGC CATAAGTGCC 
AAGCCACTGA CCCGCTACAT GCCTCAGAAC AAGCAGTCAT TTCAGTACAA GATGTGGAAA 
TTTGTGGTGT CCCCGCCCTT TGAGTATTTT ATCATGGTCA TGATTGCACT CAATACTATT 
GTTCTCATGA TGAAGTTCTA TGATGCTCCA GAAGCATATG AAGAAATGTT GAAATGCCTG 
AATATTGTCT TTACATCCAT GTTTTCAATG GAGTGTGTGC TGAAGATCAT TGCCTTTGGT 
GTGCTGAATT ATTTCCGAGA TGCCTGGAAT GTCTTTGATT TTGTAACAGT GTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATTGCG GACACGGACA ACTTCATCAA CCTCAGCTTC 
CTGCGGCTCT TCAGGGCAGC AAGACTTATC AAGCTCCTTC GCCAGGGTTA CACTATCCGC 
ATCTTGCTCT GGACGTTTGT GCAGTCATTT AAGGCCTTGC CTTATGTGTG TCTTCTCATT 
GCAATGCTCT TCTTCATCTA TGCCATTATT GGCATGCAGG TATTTGGAAA CATTGCACTA 
AATGACGAAA CCTCCATCAA TCGGCACAAT AATTTCCGTA CCTTCCTTCA AGCCTTGATG 
CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC TGGCACGAGA TCATGCTGTC ATGCCTGAGC 
AACAGGGCCT GCGACCCACT CTCCGGCCTC ACCAAGAACG AGTGCGGCAG CGAGTTCGCC 
TACTTCTACT TCGTGTCATT CATCTTCCTC TGCTCCTTCC TGATGTTGAA CCTATTTGTG 
GCTGTAATCA TGGACAACTT TGAATACTTG ACAAGAGACT CCTCCATCCT TGGGCCCCAT 
CATTTGGATG AGTTTGTCCG TGTCTGGGCT GAATACGACC CAGCTGCCTG CGGGCGCATC 
AGTTACACAG ACATGTATGA GATGCTGAGA CACATGTCCC CACCTCTAGG CCTTGGAAAG 
AAATGCCCTG CTCGCGTTGC CTATAAGCGC CTGGTGCGGA TGAACATGCC CATCTCACCC 
GAGGATCTGA CAGTCCACTT CACCTCCACG CTGATGGCCC TGATCCGCAC TGCTCTGGAA 
ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG CACCAGTGTG ATGCTGAGCT GAGAAAGGAG 
ATATCTCTAG TTTGGCCCAA CCTGTCCCAG AAGACACTGG ATTTGCTGGT GCCTCCTCAC 
AAACCTGATG AGATGACTGT GGGGAAGGTC TACGCAGCGC TCATGATATT TGACTTCTAC 
AAACAGAACA AGAACAGCAG GGAACAAGTC CACCAGCCTC CTGGGGGCCT GTGCCAGCCC 
GGACCAGTGT CACTCTTCCA CCCCTTGAAG GCCACTTTGG AGCAAACCCA GCCCTCGGCA 
TTCAGCAGTG CGAAGGCATT CCTGCGCCAG AAAAGCTCGG CCTCACTCAA CAATGGAGGC 
ACATTGCCAG CCCCAGAGGG TGGGATCAAA GAGTCCAGTT CGTGGGGGAC CCAACGGACC 
CAGGACGTGT TTTATGAAAC CAGGACACCA GCTTTTGAAC GAGGCCACTC AGAGGAAATC 
CCCATTGAAC GGGTGGTGGA AATGAGGGAA ATCAGCCCCA CACTTGCCAA TGGAGAGCAC 
CAACCAGGGC TGGAGAGCCA GGGTCGGGCG GCTTCCATGC CGCGCCTGGC TGCTGAAACG 
CAGCCCATCC CGGACACAAG CCCCATGAAG CGCTCCATCT CCACGCTGAC CCCGCAGCGT 
CCTCACCCCA TGCACCTCTA TGAGTACTCC CTGGAGCGCG TGCCCACGGA CCAGGTGCAT 
CACCACCACC ACCACCGCTG CCACCGGCGG AAAGAGAAGA AGCAGAAGTC CTTGGACAGG 
ACCACACATC AGCTGGCCGA TGGGGAAGCT GTGGCCCAGT CTGGTGAATC TTCTTCCAAG 
GACAAGAAGC AAGAGCGTGG ACGGTCCCAA GAGAGGAAGC AGCACTCTTC CTCCTCCTCA 
GAAAAGCAGC GCTTCTACTC CTGTGATCGC TATGGCAGCC GGGACCGATC CCAGCCCAAG 
TCTGCTGATC AGAGCAGGCC TACTTCACCC AATGGTGGGC CTGAGCAAGG TCCCCACAGA 
CAGGGCAGTG GTTCGGTTAA CGGGAGCCCC TTGCTGTCGA CATCTGGTGC TAGTACCCCC 
TGCCGGGGTC GGAGGCAGCT CCCGCAGACC CCCCTGACCC CACGCCCCAG CATCACTTAC 
AAGACCGCCA ACTCCTCGCC CGTACACTTC AGCACGTCGC CGGGCGGCCT CCCGCCCTTC 
TCCCCGGGCC GCCTGAGCCG CGGGCTGTCT GAGCACAATG CACTGCTGCG TGGGGACCAG 
CAGCCGCCCC CGGCCGCGGT TGCCCGCATC GGCTCCGACC CTTACCTGGG CCACCGCGAC 
GCCGCCGACA GCCCCATAGG CGCAGCGCCC GAGGACACGC TCACCTTCGA GGAAGCCGTG 
GCCACCAACT CGGGGCGTTC CTCCAGGACT TCTTACGTCT CCTCCTTGAC CTCCCAGTCT 
CACCAAGCAC GCCGGGTGCC CAATGGCTAC CATTACACGC TGGGGCTCAA CACGGGGCCC 
GGCACTGGCA CCAGGGGACG GAGCTACTAC CACGAAGCCG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279268.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134754.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X9, mRNA.

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CloneID	OGa43294
Clone ID Related Accession (Same CDS sequence)	XM_015279268.1
Accession Version	XM_015279268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCCAAGCAGC AGAACTCCTC CAAATCCAAG TCGGTTTGGG AGCAACGAAC CAGCCAGATC 
CGGATGCACA ACTTCCGAGC CAGCTGCGAG GCTCTGTACA ACGAACTGGA CCCCGAGGAG 
CGGGTGCGCT ACGCCACGAC CCTACACATC CGTCCAGACA TGAAGACCCA CTTGGATCGG 
CCACTGGTGG TAGAGCCAAG GGGTGAAGGG AGGAACAACA TCAGTAAGCT GAGCCCTGTG 
GATGTGCAGG AGGTGGAGCA GACCAAAGTC AGCTCTACCG ACGGAGCAGA AGCTCCGCGG 
AAGCATCACC GGCATCGGGA TAAGGAGAAA CTGGGAGAGC AAGAGAAGGG TGATGTGACC 
AAAGATGAGA ATGGGGAATC TGGCATCAAC AATAAGGAGG AGAGGCACAG GCAGCACAGA 
AGCCGTAGCA AGGAGGTGGA AGGGGGCAGC AAGGAAGGGA AAAGTGACCG CAGCAGGGGC 
CAGGAAGGAG GGAAGAGGCA CCATCGCCGG GGGTCGGTGG AAGAAGGCGT TGAGAAGGAG 
CACCGGAGGC ACCGCACGCA CCGGCACTCT GCAGAACGGC AAGGCAAGGA GGGCAATGGG 
ACCATCAACG GGGCCAGGAG TGAGCGGCGG ACCCGCCACC GTGGAGGGTC GAGGTCTGGG 
AACCGGGAAG GAGAGCCTGG GTCCAAGGGC GAGAATGGAG AGGAGCCTCA CAGACGGCAC 
CGGTTCAGGA GCAGGGCGCT GTCGACGTAC GATTCGGTGG AGAAGGAGAA CAGGGAGAAG 
GAAGGGGAGA CTGCTGAGAA GGAGCACCAG AATCATCAGC CCAAGGAGAA TCAGTGTGAG 
ATTGAGGCCA GTGGGAGTGT GAGCATCCCG GTGCACACCC TTCCCAGCAC CTATCTGCAG 
AAAGTGCCAG AGCAGCCTGA AGATGCTGAC AACCAGAAGA ACGTGACACG CATGATTCAG 
CCGCCTCTGG ACAAAACCAC CACTGTGAAC ATCCCCGTCA CCATCACCGC CCCGCCAGGA 
GAAACCACCG TCATACCTAT GAACAACGTT GAGTTCGAAA GTAAAACAGA AGAGAAGAAG 
GACGTTGATG ACTTGACGAA AAATGGGCCT AAACCAATCT TGCCTTACAG TTCCATGTTC 
ATCCTAAGTC CTACGAACCC GATCCGGCGT CTGTTCCACT ACATCGTCAA CCTGCGCTAC 
TTCGAGATGG TCATCCTCAT CGTCATCGCT CTCAGCAGCA TTGCCCTGGC AGCAGAAGAC 
CCCGTCCAAG CAGAATCACC AAGGAACGAT GCTCTGAAGT ACTTGGATTA TATATTCACG 
GGTGTCTTTA CCTTCGAGAT GGTGATCAAG ATGATTGACC TGGGGCTGTT ACTTCACCCT 
GGCTCCTATT TCCGTGACCT GTGGAACATC TTGGACTTCA TTGTGGTCAG TGGGGCCTTG 
GTGGCATTTG CTTTCTCAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTACATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGAGT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGTTGCC GGATTCATTA TAAGGATATG 
TACAATTTGT TACGTGTAAT TGCTCCACCC CTGGGCTTAG GAAAGAAGTG CCCTCATAGG 
GTGGCATACA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGAG GAGCAAAGCA 
CGGTCACCTG GGAGTTACCT AGCACCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGGGCAGT GGTTCGGTTA ACGGGAGCCC CTTGCTGTCG 
ACATCTGGTG CTAGTACCCC CTGCCGGGGT CGGAGGCAGC TCCCGCAGAC CCCCCTGACC 
CCACGCCCCA GCATCACTTA CAAGACCGCC AACTCCTCGC CCGTACACTT CAGCACGTCG 
CCGGGCGGCC TCCCGCCCTT CTCCCCGGGC CGCCTGAGCC GCGGGCTGTC TGAGCACAAT 
GCACTGCTGC GTGGGGACCA GCAGCCGCCC CCGGCCGCGG TTGCCCGCAT CGGCTCCGAC 
CCTTACCTGG GCCACCGCGA CGCCGCCGAC AGCCCCATAG GCGCAGCGCC CGAGGACACG 
CTCACCTTCG AGGAAGCCGT GGCCACCAAC TCGGGGCGTT CCTCCAGGAC TTCTTACGTC 
TCCTCCTTGA CCTCCCAGTC TCACCAAGCA CGCCGGGTGC CCAATGGCTA CCATTACACG 
CTGGGGCTCA ACACGGGGCC CGGCACTGGC ACCAGGGGAC GGAGCTACTA CCACGAAGCC 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134754.1
CDS	427..7467
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279268.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279268.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCCAAGCAGC AGAACTCCTC CAAATCCAAG TCGGTTTGGG AGCAACGAAC CAGCCAGATC 
CGGATGCACA ACTTCCGAGC CAGCTGCGAG GCTCTGTACA ACGAACTGGA CCCCGAGGAG 
CGGGTGCGCT ACGCCACGAC CCTACACATC CGTCCAGACA TGAAGACCCA CTTGGATCGG 
CCACTGGTGG TAGAGCCAAG GGGTGAAGGG AGGAACAACA TCAGTAAGCT GAGCCCTGTG 
GATGTGCAGG AGGTGGAGCA GACCAAAGTC AGCTCTACCG ACGGAGCAGA AGCTCCGCGG 
AAGCATCACC GGCATCGGGA TAAGGAGAAA CTGGGAGAGC AAGAGAAGGG TGATGTGACC 
AAAGATGAGA ATGGGGAATC TGGCATCAAC AATAAGGAGG AGAGGCACAG GCAGCACAGA 
AGCCGTAGCA AGGAGGTGGA AGGGGGCAGC AAGGAAGGGA AAAGTGACCG CAGCAGGGGC 
CAGGAAGGAG GGAAGAGGCA CCATCGCCGG GGGTCGGTGG AAGAAGGCGT TGAGAAGGAG 
CACCGGAGGC ACCGCACGCA CCGGCACTCT GCAGAACGGC AAGGCAAGGA GGGCAATGGG 
ACCATCAACG GGGCCAGGAG TGAGCGGCGG ACCCGCCACC GTGGAGGGTC GAGGTCTGGG 
AACCGGGAAG GAGAGCCTGG GTCCAAGGGC GAGAATGGAG AGGAGCCTCA CAGACGGCAC 
CGGTTCAGGA GCAGGGCGCT GTCGACGTAC GATTCGGTGG AGAAGGAGAA CAGGGAGAAG 
GAAGGGGAGA CTGCTGAGAA GGAGCACCAG AATCATCAGC CCAAGGAGAA TCAGTGTGAG 
ATTGAGGCCA GTGGGAGTGT GAGCATCCCG GTGCACACCC TTCCCAGCAC CTATCTGCAG 
AAAGTGCCAG AGCAGCCTGA AGATGCTGAC AACCAGAAGA ACGTGACACG CATGATTCAG 
CCGCCTCTGG ACAAAACCAC CACTGTGAAC ATCCCCGTCA CCATCACCGC CCCGCCAGGA 
GAAACCACCG TCATACCTAT GAACAACGTT GAGTTCGAAA GTAAAACAGA AGAGAAGAAG 
GACGTTGATG ACTTGACGAA AAATGGGCCT AAACCAATCT TGCCTTACAG TTCCATGTTC 
ATCCTAAGTC CTACGAACCC GATCCGGCGT CTGTTCCACT ACATCGTCAA CCTGCGCTAC 
TTCGAGATGG TCATCCTCAT CGTCATCGCT CTCAGCAGCA TTGCCCTGGC AGCAGAAGAC 
CCCGTCCAAG CAGAATCACC AAGGAACGAT GCTCTGAAGT ACTTGGATTA TATATTCACG 
GGTGTCTTTA CCTTCGAGAT GGTGATCAAG ATGATTGACC TGGGGCTGTT ACTTCACCCT 
GGCTCCTATT TCCGTGACCT GTGGAACATC TTGGACTTCA TTGTGGTCAG TGGGGCCTTG 
GTGGCATTTG CTTTCTCAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTACATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGAGT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGTTGCC GGATTCATTA TAAGGATATG 
TACAATTTGT TACGTGTAAT TGCTCCACCC CTGGGCTTAG GAAAGAAGTG CCCTCATAGG 
GTGGCATACA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGAG GAGCAAAGCA 
CGGTCACCTG GGAGTTACCT AGCACCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGGGCAGT GGTTCGGTTA ACGGGAGCCC CTTGCTGTCG 
ACATCTGGTG CTAGTACCCC CTGCCGGGGT CGGAGGCAGC TCCCGCAGAC CCCCCTGACC 
CCACGCCCCA GCATCACTTA CAAGACCGCC AACTCCTCGC CCGTACACTT CAGCACGTCG 
CCGGGCGGCC TCCCGCCCTT CTCCCCGGGC CGCCTGAGCC GCGGGCTGTC TGAGCACAAT 
GCACTGCTGC GTGGGGACCA GCAGCCGCCC CCGGCCGCGG TTGCCCGCAT CGGCTCCGAC 
CCTTACCTGG GCCACCGCGA CGCCGCCGAC AGCCCCATAG GCGCAGCGCC CGAGGACACG 
CTCACCTTCG AGGAAGCCGT GGCCACCAAC TCGGGGCGTT CCTCCAGGAC TTCTTACGTC 
TCCTCCTTGA CCTCCCAGTC TCACCAAGCA CGCCGGGTGC CCAATGGCTA CCATTACACG 
CTGGGGCTCA ACACGGGGCC CGGCACTGGC ACCAGGGGAC GGAGCTACTA CCACGAAGCC 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279269.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134755.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X10, mRNA.

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CloneID	OGa43295
Clone ID Related Accession (Same CDS sequence)	XM_015279269.1
Accession Version	XM_015279269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC TTTTGTAAAG CAAACTCGAG GTACTGTATC TCGCAGCTCG 
TCAGTCTCCA GCGTAAATTC ACCCAAGCAG CAGAACTCCT CCAAATCCAA GTCGGTTTGG 
GAGCAACGAA CCAGCCAGAT CCGGATGCAC AACTTCCGAG CCAGCTGCGA GGCTCTGTAC 
AACGAACTGG ACCCCGAGGA GCGGGTGCGC TACGCCACGA CCCTACACAT CCGTCCAGAC 
ATGAAGACCC ACTTGGATCG GCCACTGGTG GTAGAGCCAA GGGGTGAAGG GAGGAACAAC 
ATCAGTAAGC TGAGCCCTGT GGATGTGCAG GAGGTGGAGC AGACCAAAGT CAGCTCTACC 
GACGGAGCAG AAGCTCCGCG GAAGCATCAC CGGCATCGGG ATAAGGAGAA ACTGGGAGAG 
CAAGAGAAGG GTGATGTGAC CAAAGATGAG AATGGGGAAT CTGGCATCAA CAATAAGGAG 
GAGAGGCACA GGCAGCACAG AAGCCGTAGC AAGGAGGTGG AAGGGGGCAG CAAGGAAGGG 
AAAAGTGACC GCAGCAGGGG CCAGGAAGGA GGGAAGAGGC ACCATCGCCG GGGGTCGGTG 
GAAGAAGGCG TTGAGAAGGA GCACCGGAGG CACCGCACGC ACCGGCACTC TGCAGAACGG 
CAAGGCAAGG AGGGCAATGG GACCATCAAC GGGGCCAGGA GTGAGCGGCG GACCCGCCAC 
CGTGGAGGGT CGAGGTCTGG GAACCGGGAA GGAGAGCCTG GGTCCAAGGG CGAGAATGGA 
GAGGAGCCTC ACAGACGGCA CCGGTTCAGG AGCAGGGCGC TGTCGACGTA CGATTCGGTG 
GAGAAGGAGA ACAGGGAGAA GGAAGGGGAG ACTGCTGAGA AGGAGCACCA GAATCATCAG 
CCCAAGGAGA ATCAGTGTGA GATTGAGGCC AGTGGGAGTG TGAGCATCCC GGTGCACACC 
CTTCCCAGCA CCTATCTGCA GAAAGTGCCA GAGCAGCCTG AAGATGCTGA CAACCAGAAG 
AACGTGACAC GCATGATTCA GCCGCCTCTG GACAAAACCA CCACTGTGAA CATCCCCGTC 
ACCATCACCG CCCCGCCAGG AGAAACCACC GTCATACCTA TGAACAACGT TGAGTTCGAA 
AGTAAAACAG AAGAGAAGAA GGACGTTGAT GACTTGACGA AAAATGGGCC TAAACCAATC 
TTGCCTTACA GTTCCATGTT CATCCTAAGT CCTACGAACC CGATCCGGCG TCTGTTCCAC 
TACATCGTCA ACCTGCGCTA CTTCGAGATG GTCATCCTCA TCGTCATCGC TCTCAGCAGC 
ATTGCCCTGG CAGCAGAAGA CCCCGTCCAA GCAGAATCAC CAAGGAACGA TGCTCTGAAG 
TACTTGGATT ATATATTCAC GGGTGTCTTT ACCTTCGAGA TGGTGATCAA GATGATTGAC 
CTGGGGCTGT TACTTCACCC TGGCTCCTAT TTCCGTGACC TGTGGAACAT CTTGGACTTC 
ATTGTGGTCA GTGGGGCCTT GGTGGCATTT GCTTTCTCGA GTTTCATGGG AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG AGTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGCG GGCGCATCAG TTACACAGAC ATGTATGAGA TGCTGAGACA CATGTCCCCA 
CCTCTAGGCC TTGGAAAGAA ATGCCCTGCT CGCGTTGCCT ATAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACA GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACACTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AACAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ATGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCGGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GCCCATCCCG GACACAAGCC CCATGAAGCG CTCCATCTCC 
ACGCTGACCC CGCAGCGTCC TCACCCCATG CACCTCTATG AGTACTCCCT GGAGCGCGTG 
CCCACGGACC AGGTGCATCA CCACCACCAC CACCGCTGCC ACCGGCGGAA AGAGAAGAAG 
CAGAAGTCCT TGGACAGGAC CACACATCAG CTGGCCGATG GGGAAGCTGT GGCCCAGTCT 
GGTGAATCTT CTTCCAAGGA CAAGAAGCAA GAGCGTGGAC GGTCCCAAGA GAGGAAGCAG 
CACTCTTCCT CCTCCTCAGA AAAGCAGCGC TTCTACTCCT GTGATCGCTA TGGCAGCCGG 
GACCGATCCC AGCCCAAGTC TGCTGATCAG AGCAGGCCTA CTTCACCCAA TGGTGGGCCT 
GAGCAAGGTC CCCACAGACA GGGCAGTGGT TCGGTTAACG GGAGCCCCTT GCTGTCGACA 
TCTGGTGCTA GTACCCCCTG CCGGGGTCGG AGGCAGCTCC CGCAGACCCC CCTGACCCCA 
CGCCCCAGCA TCACTTACAA GACCGCCAAC TCCTCGCCCG TACACTTCAG CACGTCGCCG 
GGCGGCCTCC CGCCCTTCTC CCCGGGCCGC CTGAGCCGCG GGCTGTCTGA GCACAATGCA 
CTGCTGCGTG GGGACCAGCA GCCGCCCCCG GCCGCGGTTG CCCGCATCGG CTCCGACCCT 
TACCTGGGCC ACCGCGACGC CGCCGACAGC CCCATAGGCG CAGCGCCCGA GGACACGCTC 
ACCTTCGAGG AAGCCGTGGC CACCAACTCG GGGCGTTCCT CCAGGACTTC TTACGTCTCC 
TCCTTGACCT CCCAGTCTCA CCAAGCACGC CGGGTGCCCA ATGGCTACCA TTACACGCTG 
GGGCTCAACA CGGGGCCCGG CACTGGCACC AGGGGACGGA GCTACTACCA CGAAGCCGAC 
GAGGATGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134755.1
CDS	425..7462
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279269.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279269.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC TTTTGTAAAG CAAACTCGAG GTACTGTATC TCGCAGCTCG 
TCAGTCTCCA GCGTAAATTC ACCCAAGCAG CAGAACTCCT CCAAATCCAA GTCGGTTTGG 
GAGCAACGAA CCAGCCAGAT CCGGATGCAC AACTTCCGAG CCAGCTGCGA GGCTCTGTAC 
AACGAACTGG ACCCCGAGGA GCGGGTGCGC TACGCCACGA CCCTACACAT CCGTCCAGAC 
ATGAAGACCC ACTTGGATCG GCCACTGGTG GTAGAGCCAA GGGGTGAAGG GAGGAACAAC 
ATCAGTAAGC TGAGCCCTGT GGATGTGCAG GAGGTGGAGC AGACCAAAGT CAGCTCTACC 
GACGGAGCAG AAGCTCCGCG GAAGCATCAC CGGCATCGGG ATAAGGAGAA ACTGGGAGAG 
CAAGAGAAGG GTGATGTGAC CAAAGATGAG AATGGGGAAT CTGGCATCAA CAATAAGGAG 
GAGAGGCACA GGCAGCACAG AAGCCGTAGC AAGGAGGTGG AAGGGGGCAG CAAGGAAGGG 
AAAAGTGACC GCAGCAGGGG CCAGGAAGGA GGGAAGAGGC ACCATCGCCG GGGGTCGGTG 
GAAGAAGGCG TTGAGAAGGA GCACCGGAGG CACCGCACGC ACCGGCACTC TGCAGAACGG 
CAAGGCAAGG AGGGCAATGG GACCATCAAC GGGGCCAGGA GTGAGCGGCG GACCCGCCAC 
CGTGGAGGGT CGAGGTCTGG GAACCGGGAA GGAGAGCCTG GGTCCAAGGG CGAGAATGGA 
GAGGAGCCTC ACAGACGGCA CCGGTTCAGG AGCAGGGCGC TGTCGACGTA CGATTCGGTG 
GAGAAGGAGA ACAGGGAGAA GGAAGGGGAG ACTGCTGAGA AGGAGCACCA GAATCATCAG 
CCCAAGGAGA ATCAGTGTGA GATTGAGGCC AGTGGGAGTG TGAGCATCCC GGTGCACACC 
CTTCCCAGCA CCTATCTGCA GAAAGTGCCA GAGCAGCCTG AAGATGCTGA CAACCAGAAG 
AACGTGACAC GCATGATTCA GCCGCCTCTG GACAAAACCA CCACTGTGAA CATCCCCGTC 
ACCATCACCG CCCCGCCAGG AGAAACCACC GTCATACCTA TGAACAACGT TGAGTTCGAA 
AGTAAAACAG AAGAGAAGAA GGACGTTGAT GACTTGACGA AAAATGGGCC TAAACCAATC 
TTGCCTTACA GTTCCATGTT CATCCTAAGT CCTACGAACC CGATCCGGCG TCTGTTCCAC 
TACATCGTCA ACCTGCGCTA CTTCGAGATG GTCATCCTCA TCGTCATCGC TCTCAGCAGC 
ATTGCCCTGG CAGCAGAAGA CCCCGTCCAA GCAGAATCAC CAAGGAACGA TGCTCTGAAG 
TACTTGGATT ATATATTCAC GGGTGTCTTT ACCTTCGAGA TGGTGATCAA GATGATTGAC 
CTGGGGCTGT TACTTCACCC TGGCTCCTAT TTCCGTGACC TGTGGAACAT CTTGGACTTC 
ATTGTGGTCA GTGGGGCCTT GGTGGCATTT GCTTTCTCGA GTTTCATGGG AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG AGTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGCG GGCGCATCAG TTACACAGAC ATGTATGAGA TGCTGAGACA CATGTCCCCA 
CCTCTAGGCC TTGGAAAGAA ATGCCCTGCT CGCGTTGCCT ATAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACA GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACACTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AACAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ATGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCGGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GCCCATCCCG GACACAAGCC CCATGAAGCG CTCCATCTCC 
ACGCTGACCC CGCAGCGTCC TCACCCCATG CACCTCTATG AGTACTCCCT GGAGCGCGTG 
CCCACGGACC AGGTGCATCA CCACCACCAC CACCGCTGCC ACCGGCGGAA AGAGAAGAAG 
CAGAAGTCCT TGGACAGGAC CACACATCAG CTGGCCGATG GGGAAGCTGT GGCCCAGTCT 
GGTGAATCTT CTTCCAAGGA CAAGAAGCAA GAGCGTGGAC GGTCCCAAGA GAGGAAGCAG 
CACTCTTCCT CCTCCTCAGA AAAGCAGCGC TTCTACTCCT GTGATCGCTA TGGCAGCCGG 
GACCGATCCC AGCCCAAGTC TGCTGATCAG AGCAGGCCTA CTTCACCCAA TGGTGGGCCT 
GAGCAAGGTC CCCACAGACA GGGCAGTGGT TCGGTTAACG GGAGCCCCTT GCTGTCGACA 
TCTGGTGCTA GTACCCCCTG CCGGGGTCGG AGGCAGCTCC CGCAGACCCC CCTGACCCCA 
CGCCCCAGCA TCACTTACAA GACCGCCAAC TCCTCGCCCG TACACTTCAG CACGTCGCCG 
GGCGGCCTCC CGCCCTTCTC CCCGGGCCGC CTGAGCCGCG GGCTGTCTGA GCACAATGCA 
CTGCTGCGTG GGGACCAGCA GCCGCCCCCG GCCGCGGTTG CCCGCATCGG CTCCGACCCT 
TACCTGGGCC ACCGCGACGC CGCCGACAGC CCCATAGGCG CAGCGCCCGA GGACACGCTC 
ACCTTCGAGG AAGCCGTGGC CACCAACTCG GGGCGTTCCT CCAGGACTTC TTACGTCTCC 
TCCTTGACCT CCCAGTCTCA CCAAGCACGC CGGGTGCCCA ATGGCTACCA TTACACGCTG 
GGGCTCAACA CGGGGCCCGG CACTGGCACC AGGGGACGGA GCTACTACCA CGAAGCCGAC 
GAGGATGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279270.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134756.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X11, mRNA.

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CloneID	OGa43296
Clone ID Related Accession (Same CDS sequence)	XM_015279270.1
Accession Version	XM_015279270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CAACTTCATC 
AACCTCAGCT TCCTGCGGCT CTTCAGGGCA GCAAGACTTA TCAAGCTCCT TCGCCAGGGT 
TACACTATCC GCATCTTGCT CTGGACGTTT GTGCAGTCAT TTAAGGCCTT GCCTTATGTG 
TGTCTTCTCA TTGCAATGCT CTTCTTCATC TATGCCATTA TTGGCATGCA GGTATTTGGA 
AACATTGCAC TAAATGACGA AACCTCCATC AATCGGCACA ATAATTTCCG TACCTTCCTT 
CAAGCCTTGA TGCTTCTGTT CAGGAGTGCC ACGGGGGAGG CCTGGCACGA GATCATGCTG 
TCATGCCTGA GCAACAGGGC CTGCGACCCA CTCTCCGGCC TCACCAAGAA CGAGTGCGGC 
AGCGAGTTCG CCTACTTCTA CTTCGTGTCA TTCATCTTCC TCTGCTCCTT CCTGATGTTG 
AACCTATTTG TGGCTGTAAT CATGGACAAC TTTGAATACT TGACAAGAGA CTCCTCCATC 
CTTGGGCCCC ATCATTTGGA TGAGTTTGTC CGTGTCTGGG CTGAATACGA CCCAGCTGCC 
TGCGGGCGCA TCAGTTACAC AGACATGTAT GAGATGCTGA GACACATGTC CCCACCTCTA 
GGCCTTGGAA AGAAATGCCC TGCTCGCGTT GCCTATAAGC GCCTGGTGCG GATGAACATG 
CCCATCTCAC CCGAGGATCT GACAGTCCAC TTCACCTCCA CGCTGATGGC CCTGATCCGC 
ACTGCTCTGG AAATCAAGCT CGCCTCAGGG GGTGTTAAGC AGCACCAGTG TGATGCTGAG 
CTGAGAAAGG AGATATCTCT AGTTTGGCCC AACCTGTCCC AGAAGACACT GGATTTGCTG 
GTGCCTCCTC ACAAACCTGA TGAGATGACT GTGGGGAAGG TCTACGCAGC GCTCATGATA 
TTTGACTTCT ACAAACAGAA CAAGAACAGC AGGGAACAAG TCCACCAGCC TCCTGGGGGC 
CTGTGCCAGC CCGGACCAGT GTCACTCTTC CACCCCTTGA AGGCCACTTT GGAGCAAACC 
CAGCCCTCGG CATTCAGCAG TGCGAAGGCA TTCCTGCGCC AGAAAAGCTC GGCCTCACTC 
AACAATGGAG GCACATTGCC AGCCCCAGAG GGTGGGATCA AAGAGTCCAG TTCGTGGGGG 
ACCCAACGGA CCCAGGACGT GTTTTATGAA ACCAGGACAC CAGCTTTTGA ACGAGGCCAC 
TCAGAGGAAA TCCCCATTGA ACGGGTGGTG GAAATGAGGG AAATCAGCCC CACACTTGCC 
AATGGAGAGC ACCAACCAGG GCTGGAGAGC CAGGGTCGGG CGGCTTCCAT GCCGCGCCTG 
GCTGCTGAAA CGCAGCCCAT CCCGGACACA AGCCCCATGA AGCGCTCCAT CTCCACGCTG 
ACCCCGCAGC GTCCTCACCC CATGCACCTC TATGAGTACT CCCTGGAGCG CGTGCCCACG 
GACCAGGTGC ATCACCACCA CCACCACCGC TGCCACCGGC GGAAAGAGAA GAAGCAGAAG 
TCCTTGGACA GGACCACACA TCAGCTGGCC GATGGGGAAG CTGTGGCCCA GTCTGGTGAA 
TCTTCTTCCA AGGACAAGAA GCAAGAGCGT GGACGGTCCC AAGAGAGGAA GCAGCACTCT 
TCCTCCTCCT CAGAAAAGCA GCGCTTCTAC TCCTGTGATC GCTATGGCAG CCGGGACCGA 
TCCCAGCCCA AGTCTGCTGA TCAGAGCAGG CCTACTTCAC CCAATGGTGG GCCTGAGCAA 
GGTCCCCACA GACAGGGCAG TGGTTCGGTT AACGGGAGCC CCTTGCTGTC GACATCTGGT 
GCTAGTACCC CCTGCCGGGG TCGGAGGCAG CTCCCGCAGA CCCCCCTGAC CCCACGCCCC 
AGCATCACTT ACAAGACCGC CAACTCCTCG CCCGTACACT TCAGCACGTC GCCGGGCGGC 
CTCCCGCCCT TCTCCCCGGG CCGCCTGAGC CGCGGGCTGT CTGAGCACAA TGCACTGCTG 
CGTGGGGACC AGCAGCCGCC CCCGGCCGCG GTTGCCCGCA TCGGCTCCGA CCCTTACCTG 
GGCCACCGCG ACGCCGCCGA CAGCCCCATA GGCGCAGCGC CCGAGGACAC GCTCACCTTC 
GAGGAAGCCG TGGCCACCAA CTCGGGGCGT TCCTCCAGGA CTTCTTACGT CTCCTCCTTG 
ACCTCCCAGT CTCACCAAGC ACGCCGGGTG CCCAATGGCT ACCATTACAC GCTGGGGCTC 
AACACGGGGC CCGGCACTGG CACCAGGGGA CGGAGCTACT ACCACGAAGC CGACGAGGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134756.1
CDS	425..7456
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279270.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279270.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CAACTTCATC 
AACCTCAGCT TCCTGCGGCT CTTCAGGGCA GCAAGACTTA TCAAGCTCCT TCGCCAGGGT 
TACACTATCC GCATCTTGCT CTGGACGTTT GTGCAGTCAT TTAAGGCCTT GCCTTATGTG 
TGTCTTCTCA TTGCAATGCT CTTCTTCATC TATGCCATTA TTGGCATGCA GGTATTTGGA 
AACATTGCAC TAAATGACGA AACCTCCATC AATCGGCACA ATAATTTCCG TACCTTCCTT 
CAAGCCTTGA TGCTTCTGTT CAGGAGTGCC ACGGGGGAGG CCTGGCACGA GATCATGCTG 
TCATGCCTGA GCAACAGGGC CTGCGACCCA CTCTCCGGCC TCACCAAGAA CGAGTGCGGC 
AGCGAGTTCG CCTACTTCTA CTTCGTGTCA TTCATCTTCC TCTGCTCCTT CCTGATGTTG 
AACCTATTTG TGGCTGTAAT CATGGACAAC TTTGAATACT TGACAAGAGA CTCCTCCATC 
CTTGGGCCCC ATCATTTGGA TGAGTTTGTC CGTGTCTGGG CTGAATACGA CCCAGCTGCC 
TGCGGGCGCA TCAGTTACAC AGACATGTAT GAGATGCTGA GACACATGTC CCCACCTCTA 
GGCCTTGGAA AGAAATGCCC TGCTCGCGTT GCCTATAAGC GCCTGGTGCG GATGAACATG 
CCCATCTCAC CCGAGGATCT GACAGTCCAC TTCACCTCCA CGCTGATGGC CCTGATCCGC 
ACTGCTCTGG AAATCAAGCT CGCCTCAGGG GGTGTTAAGC AGCACCAGTG TGATGCTGAG 
CTGAGAAAGG AGATATCTCT AGTTTGGCCC AACCTGTCCC AGAAGACACT GGATTTGCTG 
GTGCCTCCTC ACAAACCTGA TGAGATGACT GTGGGGAAGG TCTACGCAGC GCTCATGATA 
TTTGACTTCT ACAAACAGAA CAAGAACAGC AGGGAACAAG TCCACCAGCC TCCTGGGGGC 
CTGTGCCAGC CCGGACCAGT GTCACTCTTC CACCCCTTGA AGGCCACTTT GGAGCAAACC 
CAGCCCTCGG CATTCAGCAG TGCGAAGGCA TTCCTGCGCC AGAAAAGCTC GGCCTCACTC 
AACAATGGAG GCACATTGCC AGCCCCAGAG GGTGGGATCA AAGAGTCCAG TTCGTGGGGG 
ACCCAACGGA CCCAGGACGT GTTTTATGAA ACCAGGACAC CAGCTTTTGA ACGAGGCCAC 
TCAGAGGAAA TCCCCATTGA ACGGGTGGTG GAAATGAGGG AAATCAGCCC CACACTTGCC 
AATGGAGAGC ACCAACCAGG GCTGGAGAGC CAGGGTCGGG CGGCTTCCAT GCCGCGCCTG 
GCTGCTGAAA CGCAGCCCAT CCCGGACACA AGCCCCATGA AGCGCTCCAT CTCCACGCTG 
ACCCCGCAGC GTCCTCACCC CATGCACCTC TATGAGTACT CCCTGGAGCG CGTGCCCACG 
GACCAGGTGC ATCACCACCA CCACCACCGC TGCCACCGGC GGAAAGAGAA GAAGCAGAAG 
TCCTTGGACA GGACCACACA TCAGCTGGCC GATGGGGAAG CTGTGGCCCA GTCTGGTGAA 
TCTTCTTCCA AGGACAAGAA GCAAGAGCGT GGACGGTCCC AAGAGAGGAA GCAGCACTCT 
TCCTCCTCCT CAGAAAAGCA GCGCTTCTAC TCCTGTGATC GCTATGGCAG CCGGGACCGA 
TCCCAGCCCA AGTCTGCTGA TCAGAGCAGG CCTACTTCAC CCAATGGTGG GCCTGAGCAA 
GGTCCCCACA GACAGGGCAG TGGTTCGGTT AACGGGAGCC CCTTGCTGTC GACATCTGGT 
GCTAGTACCC CCTGCCGGGG TCGGAGGCAG CTCCCGCAGA CCCCCCTGAC CCCACGCCCC 
AGCATCACTT ACAAGACCGC CAACTCCTCG CCCGTACACT TCAGCACGTC GCCGGGCGGC 
CTCCCGCCCT TCTCCCCGGG CCGCCTGAGC CGCGGGCTGT CTGAGCACAA TGCACTGCTG 
CGTGGGGACC AGCAGCCGCC CCCGGCCGCG GTTGCCCGCA TCGGCTCCGA CCCTTACCTG 
GGCCACCGCG ACGCCGCCGA CAGCCCCATA GGCGCAGCGC CCGAGGACAC GCTCACCTTC 
GAGGAAGCCG TGGCCACCAA CTCGGGGCGT TCCTCCAGGA CTTCTTACGT CTCCTCCTTG 
ACCTCCCAGT CTCACCAAGC ACGCCGGGTG CCCAATGGCT ACCATTACAC GCTGGGGCTC 
AACACGGGGC CCGGCACTGG CACCAGGGGA CGGAGCTACT ACCACGAAGC CGACGAGGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279271.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134757.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X12, mRNA.

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CloneID	OGa43297
Clone ID Related Accession (Same CDS sequence)	XM_015279271.1
Accession Version	XM_015279271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCTTT TGTAAAGCAA 
ACTCGAGGTA CTGTATCTCG CAGCTCGTCA GTCTCCAGCG TAAATTCACC CAAGCAGCAG 
AACTCCTCCA AATCCAAGTC GGTTTGGGAG CAACGAACCA GCCAGATCCG GATGCACAAC 
TTCCGAGCCA GCTGCGAGGC TCTGTACAAC GAACTGGACC CCGAGGAGCG GGTGCGCTAC 
GCCACGACCC TACACATCCG TCCAGACATG AAGACCCACT TGGATCGGCC ACTGGTGGTA 
GAGCCAAGGG GTGAAGGGAG GAACAACATC AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG 
GTGGAGCAGA CCAAAGTCAG CTCTACCGAC GGAGCAGAAG CTCCGCGGAA GCATCACCGG 
CATCGGGATA AGGAGAAACT GGGAGAGCAA GAGAAGGGTG ATGTGACCAA AGATGAGAAT 
GGGGAATCTG GCATCAACAA TAAGGAGGAG AGGCACAGGC AGCACAGAAG CCGTAGCAAG 
GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA AGTGACCGCA GCAGGGGCCA GGAAGGAGGG 
AAGAGGCACC ATCGCCGGGG GTCGGTGGAA GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC 
CGCACGCACC GGCACTCTGC AGAACGGCAA GGCAAGGAGG GCAATGGGAC CATCAACGGG 
GCCAGGAGTG AGCGGCGGAC CCGCCACCGT GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA 
GAGCCTGGGT CCAAGGGCGA GAATGGAGAG GAGCCTCACA GACGGCACCG GTTCAGGAGC 
AGGGCGCTGT CGACGTACGA TTCGGTGGAG AAGGAGAACA GGGAGAAGGA AGGGGAGACT 
GCTGAGAAGG AGCACCAGAA TCATCAGCCC AAGGAGAATC AGTGTGAGAT TGAGGCCAGT 
GGGAGTGTGA GCATCCCGGT GCACACCCTT CCCAGCACCT ATCTGCAGAA AGTGCCAGAG 
CAGCCTGAAG ATGCTGACAA CCAGAAGAAC GTGACACGCA TGATTCAGCC GCCTCTGGAC 
AAAACCACCA CTGTGAACAT CCCCGTCACC ATCACCGCCC CGCCAGGAGA AACCACCGTC 
ATACCTATGA ACAACGTTGA GTTCGAAAGT AAAACAGAAG AGAAGAAGGA CGTTGATGAC 
TTGACGAAAA ATGGGCCTAA ACCAATCTTG CCTTACAGTT CCATGTTCAT CCTAAGTCCT 
ACGAACCCGA TCCGGCGTCT GTTCCACTAC ATCGTCAACC TGCGCTACTT CGAGATGGTC 
ATCCTCATCG TCATCGCTCT CAGCAGCATT GCCCTGGCAG CAGAAGACCC CGTCCAAGCA 
GAATCACCAA GGAACGATGC TCTGAAGTAC TTGGATTATA TATTCACGGG TGTCTTTACC 
TTCGAGATGG TGATCAAGAT GATTGACCTG GGGCTGTTAC TTCACCCTGG CTCCTATTTC 
CGTGACCTGT GGAACATCTT GGACTTCATT GTGGTCAGTG GGGCCTTGGT GGCATTTGCT 
TTCTCGAGTT TCATGGGAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTACATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGAGT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGCGGGC GCATCAGTTA CACAGACATG 
TATGAGATGC TGAGACACAT GTCCCCACCT CTAGGCCTTG GAAAGAAATG CCCTGCTCGC 
GTTGCCTATA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGCC CATCCCGGAC 
ACAAGCCCCA TGAAGCGCTC CATCTCCACG CTGACCCCGC AGCGTCCTCA CCCCATGCAC 
CTCTATGAGT ACTCCCTGGA GCGCGTGCCC ACGGACCAGG TGCATCACCA CCACCACCAC 
CGCTGCCACC GGCGGAAAGA GAAGAAGCAG AAGTCCTTGG ACAGGACCAC ACATCAGCTG 
GCCGATGGGG AAGCTGTGGC CCAGTCTGGT GAATCTTCTT CCAAGGACAA GAAGCAAGAG 
CGTGGACGGT CCCAAGAGAG GAAGCAGCAC TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC 
TACTCCTGTG ATCGCTATGG CAGCCGGGAC CGATCCCAGC CCAAGTCTGC TGATCAGAGC 
AGGCCTACTT CACCCAATGG TGGGCCTGAG CAAGGTCCCC ACAGACAGGG CAGTGGTTCG 
GTTAACGGGA GCCCCTTGCT GTCGACATCT GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG 
CAGCTCCCGC AGACCCCCCT GACCCCACGC CCCAGCATCA CTTACAAGAC CGCCAACTCC 
TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG 
AGCCGCGGGC TGTCTGAGCA CAATGCACTG CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC 
GCGGTTGCCC GCATCGGCTC CGACCCTTAC CTGGGCCACC GCGACGCCGC CGACAGCCCC 
ATAGGCGCAG CGCCCGAGGA CACGCTCACC TTCGAGGAAG CCGTGGCCAC CAACTCGGGG 
CGTTCCTCCA GGACTTCTTA CGTCTCCTCC TTGACCTCCC AGTCTCACCA AGCACGCCGG 
GTGCCCAATG GCTACCATTA CACGCTGGGG CTCAACACGG GGCCCGGCAC TGGCACCAGG 
GGACGGAGCT ACTACCACGA AGCCGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134757.1
CDS	425..7429
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279271.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279271.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCTTT TGTAAAGCAA 
ACTCGAGGTA CTGTATCTCG CAGCTCGTCA GTCTCCAGCG TAAATTCACC CAAGCAGCAG 
AACTCCTCCA AATCCAAGTC GGTTTGGGAG CAACGAACCA GCCAGATCCG GATGCACAAC 
TTCCGAGCCA GCTGCGAGGC TCTGTACAAC GAACTGGACC CCGAGGAGCG GGTGCGCTAC 
GCCACGACCC TACACATCCG TCCAGACATG AAGACCCACT TGGATCGGCC ACTGGTGGTA 
GAGCCAAGGG GTGAAGGGAG GAACAACATC AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG 
GTGGAGCAGA CCAAAGTCAG CTCTACCGAC GGAGCAGAAG CTCCGCGGAA GCATCACCGG 
CATCGGGATA AGGAGAAACT GGGAGAGCAA GAGAAGGGTG ATGTGACCAA AGATGAGAAT 
GGGGAATCTG GCATCAACAA TAAGGAGGAG AGGCACAGGC AGCACAGAAG CCGTAGCAAG 
GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA AGTGACCGCA GCAGGGGCCA GGAAGGAGGG 
AAGAGGCACC ATCGCCGGGG GTCGGTGGAA GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC 
CGCACGCACC GGCACTCTGC AGAACGGCAA GGCAAGGAGG GCAATGGGAC CATCAACGGG 
GCCAGGAGTG AGCGGCGGAC CCGCCACCGT GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA 
GAGCCTGGGT CCAAGGGCGA GAATGGAGAG GAGCCTCACA GACGGCACCG GTTCAGGAGC 
AGGGCGCTGT CGACGTACGA TTCGGTGGAG AAGGAGAACA GGGAGAAGGA AGGGGAGACT 
GCTGAGAAGG AGCACCAGAA TCATCAGCCC AAGGAGAATC AGTGTGAGAT TGAGGCCAGT 
GGGAGTGTGA GCATCCCGGT GCACACCCTT CCCAGCACCT ATCTGCAGAA AGTGCCAGAG 
CAGCCTGAAG ATGCTGACAA CCAGAAGAAC GTGACACGCA TGATTCAGCC GCCTCTGGAC 
AAAACCACCA CTGTGAACAT CCCCGTCACC ATCACCGCCC CGCCAGGAGA AACCACCGTC 
ATACCTATGA ACAACGTTGA GTTCGAAAGT AAAACAGAAG AGAAGAAGGA CGTTGATGAC 
TTGACGAAAA ATGGGCCTAA ACCAATCTTG CCTTACAGTT CCATGTTCAT CCTAAGTCCT 
ACGAACCCGA TCCGGCGTCT GTTCCACTAC ATCGTCAACC TGCGCTACTT CGAGATGGTC 
ATCCTCATCG TCATCGCTCT CAGCAGCATT GCCCTGGCAG CAGAAGACCC CGTCCAAGCA 
GAATCACCAA GGAACGATGC TCTGAAGTAC TTGGATTATA TATTCACGGG TGTCTTTACC 
TTCGAGATGG TGATCAAGAT GATTGACCTG GGGCTGTTAC TTCACCCTGG CTCCTATTTC 
CGTGACCTGT GGAACATCTT GGACTTCATT GTGGTCAGTG GGGCCTTGGT GGCATTTGCT 
TTCTCGAGTT TCATGGGAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTACATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGAGT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGCGGGC GCATCAGTTA CACAGACATG 
TATGAGATGC TGAGACACAT GTCCCCACCT CTAGGCCTTG GAAAGAAATG CCCTGCTCGC 
GTTGCCTATA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGCC CATCCCGGAC 
ACAAGCCCCA TGAAGCGCTC CATCTCCACG CTGACCCCGC AGCGTCCTCA CCCCATGCAC 
CTCTATGAGT ACTCCCTGGA GCGCGTGCCC ACGGACCAGG TGCATCACCA CCACCACCAC 
CGCTGCCACC GGCGGAAAGA GAAGAAGCAG AAGTCCTTGG ACAGGACCAC ACATCAGCTG 
GCCGATGGGG AAGCTGTGGC CCAGTCTGGT GAATCTTCTT CCAAGGACAA GAAGCAAGAG 
CGTGGACGGT CCCAAGAGAG GAAGCAGCAC TCTTCCTCCT CCTCAGAAAA GCAGCGCTTC 
TACTCCTGTG ATCGCTATGG CAGCCGGGAC CGATCCCAGC CCAAGTCTGC TGATCAGAGC 
AGGCCTACTT CACCCAATGG TGGGCCTGAG CAAGGTCCCC ACAGACAGGG CAGTGGTTCG 
GTTAACGGGA GCCCCTTGCT GTCGACATCT GGTGCTAGTA CCCCCTGCCG GGGTCGGAGG 
CAGCTCCCGC AGACCCCCCT GACCCCACGC CCCAGCATCA CTTACAAGAC CGCCAACTCC 
TCGCCCGTAC ACTTCAGCAC GTCGCCGGGC GGCCTCCCGC CCTTCTCCCC GGGCCGCCTG 
AGCCGCGGGC TGTCTGAGCA CAATGCACTG CTGCGTGGGG ACCAGCAGCC GCCCCCGGCC 
GCGGTTGCCC GCATCGGCTC CGACCCTTAC CTGGGCCACC GCGACGCCGC CGACAGCCCC 
ATAGGCGCAG CGCCCGAGGA CACGCTCACC TTCGAGGAAG CCGTGGCCAC CAACTCGGGG 
CGTTCCTCCA GGACTTCTTA CGTCTCCTCC TTGACCTCCC AGTCTCACCA AGCACGCCGG 
GTGCCCAATG GCTACCATTA CACGCTGGGG CTCAACACGG GGCCCGGCAC TGGCACCAGG 
GGACGGAGCT ACTACCACGA AGCCGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279272.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134758.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X13, mRNA.

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CloneID	OGa43298
Clone ID Related Accession (Same CDS sequence)	XM_015279272.1
Accession Version	XM_015279272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ACATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGAGTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGTTGCCGG 
ATTCATTATA AGGATATGTA CAATTTGTTA CGTGTAATTG CTCCACCCCT GGGCTTAGGA 
AAGAAGTGCC CTCATAGGGT GGCATACAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGAGGA GCAAAGCACG GTCACCTGGG AGTTACCTAG CACCCATCCC GGACACAAGC 
CCCATGAAGC GCTCCATCTC CACGCTGACC CCGCAGCGTC CTCACCCCAT GCACCTCTAT 
GAGTACTCCC TGGAGCGCGT GCCCACGGAC CAGGTGCATC ACCACCACCA CCACCGCTGC 
CACCGGCGGA AAGAGAAGAA GCAGAAGTCC TTGGACAGGA CCACACATCA GCTGGCCGAT 
GGGGAAGCTG TGGCCCAGTC TGGTGAATCT TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA 
CGGTCCCAAG AGAGGAAGCA GCACTCTTCC TCCTCCTCAG AAAAGCAGCG CTTCTACTCC 
TGTGATCGCT ATGGCAGCCG GGACCGATCC CAGCCCAAGT CTGCTGATCA GAGCAGGCCT 
ACTTCACCCA ATGGTGGGCC TGAGCAAGGT CCCCACAGAC AGGGCAGTGG TTCGGTTAAC 
GGGAGCCCCT TGCTGTCGAC ATCTGGTGCT AGTACCCCCT GCCGGGGTCG GAGGCAGCTC 
CCGCAGACCC CCCTGACCCC ACGCCCCAGC ATCACTTACA AGACCGCCAA CTCCTCGCCC 
GTACACTTCA GCACGTCGCC GGGCGGCCTC CCGCCCTTCT CCCCGGGCCG CCTGAGCCGC 
GGGCTGTCTG AGCACAATGC ACTGCTGCGT GGGGACCAGC AGCCGCCCCC GGCCGCGGTT 
GCCCGCATCG GCTCCGACCC TTACCTGGGC CACCGCGACG CCGCCGACAG CCCCATAGGC 
GCAGCGCCCG AGGACACGCT CACCTTCGAG GAAGCCGTGG CCACCAACTC GGGGCGTTCC 
TCCAGGACTT CTTACGTCTC CTCCTTGACC TCCCAGTCTC ACCAAGCACG CCGGGTGCCC 
AATGGCTACC ATTACACGCT GGGGCTCAAC ACGGGGCCCG GCACTGGCAC CAGGGGACGG 
AGCTACTACC ACGAAGCCGA CGAGGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134758.1
CDS	427..7425
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279272.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279272.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ACATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGAGTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGTTGCCGG 
ATTCATTATA AGGATATGTA CAATTTGTTA CGTGTAATTG CTCCACCCCT GGGCTTAGGA 
AAGAAGTGCC CTCATAGGGT GGCATACAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGAGGA GCAAAGCACG GTCACCTGGG AGTTACCTAG CACCCATCCC GGACACAAGC 
CCCATGAAGC GCTCCATCTC CACGCTGACC CCGCAGCGTC CTCACCCCAT GCACCTCTAT 
GAGTACTCCC TGGAGCGCGT GCCCACGGAC CAGGTGCATC ACCACCACCA CCACCGCTGC 
CACCGGCGGA AAGAGAAGAA GCAGAAGTCC TTGGACAGGA CCACACATCA GCTGGCCGAT 
GGGGAAGCTG TGGCCCAGTC TGGTGAATCT TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA 
CGGTCCCAAG AGAGGAAGCA GCACTCTTCC TCCTCCTCAG AAAAGCAGCG CTTCTACTCC 
TGTGATCGCT ATGGCAGCCG GGACCGATCC CAGCCCAAGT CTGCTGATCA GAGCAGGCCT 
ACTTCACCCA ATGGTGGGCC TGAGCAAGGT CCCCACAGAC AGGGCAGTGG TTCGGTTAAC 
GGGAGCCCCT TGCTGTCGAC ATCTGGTGCT AGTACCCCCT GCCGGGGTCG GAGGCAGCTC 
CCGCAGACCC CCCTGACCCC ACGCCCCAGC ATCACTTACA AGACCGCCAA CTCCTCGCCC 
GTACACTTCA GCACGTCGCC GGGCGGCCTC CCGCCCTTCT CCCCGGGCCG CCTGAGCCGC 
GGGCTGTCTG AGCACAATGC ACTGCTGCGT GGGGACCAGC AGCCGCCCCC GGCCGCGGTT 
GCCCGCATCG GCTCCGACCC TTACCTGGGC CACCGCGACG CCGCCGACAG CCCCATAGGC 
GCAGCGCCCG AGGACACGCT CACCTTCGAG GAAGCCGTGG CCACCAACTC GGGGCGTTCC 
TCCAGGACTT CTTACGTCTC CTCCTTGACC TCCCAGTCTC ACCAAGCACG CCGGGTGCCC 
AATGGCTACC ATTACACGCT GGGGCTCAAC ACGGGGCCCG GCACTGGCAC CAGGGGACGG 
AGCTACTACC ACGAAGCCGA CGAGGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279273.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134759.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X14, mRNA.

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CloneID	OGa43299
Clone ID Related Accession (Same CDS sequence)	XM_015279273.1
Accession Version	XM_015279273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCGAGTT TCATGGGAGG AACCAAAGGC 
AAGGACATCA ACACGATCAA GTCGCTGCGG GTTCTGCGGG TCCTACGACC ACTGAAGACC 
ATTAAACGGC TGCCAAAACT AAAGGCTGTC TTTGACTGCG TGGTGAATTC CCTGAAGAAC 
GTCCTCAACA TCCTCATTGT CTACATGCTC TTCATGTTCA TCTTTGCTGT CATTGCTGTG 
CAGCTCTTCA AAGGCAGATT CTTCTACTGC ACTGATGAGT CGAAAGAGCT GGAGAAGGAT 
TGCAGGGGAC AATACCTTGA CTATGAAAAA AATGAGGTGG AGGCGCAACC CAGGGAATGG 
AAAAAATATG AGTTCCATTA CGACAATGTG CTGTGGGCTC TGCTGACACT TTTCACTGTC 
TCCACAGGAG AAGGGTGGCC AACTGTGCTG AAGCACTCAG TGGATGCTAC CTACGAGGAA 
CAGGGTCCCA GCCCTGGCTA CCGAATGGAA ATGTCTATCT TTTACGTGGT GTATTTTGTT 
GTCTTCCCTT TTTTCTTTGT GAACATCTTT GTGGCGTTAA TCATAATCAC CTTCCAAGAG 
CAAGGAGATA AAGTGATGTC AGAATGCAGC CTAGAGAAAA ATGAGAGGGC CTGCATTGAC 
TTCGCCATAA GTGCCAAGCC ACTGACCCGC TACATGCCTC AGAACAAGCA GTCATTTCAG 
TACAAGATGT GGAAATTTGT GGTGTCCCCG CCCTTTGAGT ATTTTATCAT GGTCATGATT 
GCACTCAATA CTATTGTTCT CATGATGAAG TTCTATGATG CTCCAGAAGC ATATGAAGAA 
ATGTTGAAAT GCCTGAATAT TGTCTTTACA TCCATGTTTT CAATGGAGTG TGTGCTGAAG 
ATCATTGCCT TTGGTGTGCT GAATTATTTC CGAGATGCCT GGAATGTCTT TGATTTTGTA 
ACAGTGTTGG GAAGTATTAC TGATATTTTA GTAACAGAGA TTGCGGACAA CTTCATCAAC 
CTCAGCTTCC TGCGGCTCTT CAGGGCAGCA AGACTTATCA AGCTCCTTCG CCAGGGTTAC 
ACTATCCGCA TCTTGCTCTG GACGTTTGTG CAGTCATTTA AGGCCTTGCC TTATGTGTGT 
CTTCTCATTG CAATGCTCTT CTTCATCTAT GCCATTATTG GCATGCAGGT ATTTGGAAAC 
ATTGCACTAA ATGACGAAAC CTCCATCAAT CGGCACAATA ATTTCCGTAC CTTCCTTCAA 
GCCTTGATGC TTCTGTTCAG GAGTGCCACG GGGGAGGCCT GGCACGAGAT CATGCTGTCA 
TGCCTGAGCA ACAGGGCCTG CGACCCACTC TCCGGCCTCA CCAAGAACGA GTGCGGCAGC 
GAGTTCGCCT ACTTCTACTT CGTGTCATTC ATCTTCCTCT GCTCCTTCCT GATGTTGAAC 
CTATTTGTGG CTGTAATCAT GGACAACTTT GAATACTTGA CAAGAGACTC CTCCATCCTT 
GGGCCCCATC ATTTGGATGA GTTTGTCCGT GTCTGGGCTG AATACGACCC AGCTGCCTGC 
GGGCGCATCA GTTACACAGA CATGTATGAG ATGCTGAGAC ACATGTCCCC ACCTCTAGGC 
CTTGGAAAGA AATGCCCTGC TCGCGTTGCC TATAAGCGCC TGGTGCGGAT GAACATGCCC 
ATCTCACCCG AGGATCTGAC AGTCCACTTC ACCTCCACGC TGATGGCCCT GATCCGCACT 
GCTCTGGAAA TCAAGCTCGC CTCAGGGGGT GTTAAGCAGC ACCAGTGTGA TGCTGAGCTG 
AGAAAGGAGA TATCTCTAGT TTGGCCCAAC CTGTCCCAGA AGACACTGGA TTTGCTGGTG 
CCTCCTCACA AACCTGATGA GATGACTGTG GGGAAGGTCT ACGCAGCGCT CATGATATTT 
GACTTCTACA AACAGAACAA GAACAGCAGG GAACAAGTCC ACCAGCCTCC TGGGGGCCTG 
TGCCAGCCCG GACCAGTGTC ACTCTTCCAC CCCTTGAAGG CCACTTTGGA GCAAACCCAG 
CCCTCGGCAT TCAGCAGTGC GAAGGCATTC CTGCGCCAGA AAAGCTCGGC CTCACTCAAC 
AATGGAGGCA CATTGCCAGC CCCAGAGGGT GGGATCAAAG AGTCCAGTTC GTGGGGGACC 
CAACGGACCC AGGACGTGTT TTATGAAACC AGGACACCAG CTTTTGAACG AGGCCACTCA 
GAGGAAATCC CCATTGAACG GGTGGTGGAA ATGAGGGAAA TCAGCCCCAC ACTTGCCAAT 
GGAGAGCACC AACCAGGGCT GGAGAGCCAG GGTCGGGCGG CTTCCATGCC GCGCCTGGCT 
GCTGAAACGC AGCCCATCCC GGACACAAGC CCCATGAAGC GCTCCATCTC CACGCTGACC 
CCGCAGCGTC CTCACCCCAT GCACCTCTAT GAGTACTCCC TGGAGCGCGT GCCCACGGAC 
CAGGTGCATC ACCACCACCA CCACCGCTGC CACCGGCGGA AAGAGAAGAA GCAGAAGTCC 
TTGGACAGGA CCACACATCA GCTGGCCGAT GGGGAAGCTG TGGCCCAGTC TGGTGAATCT 
TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA CGGTCCCAAG AGAGGAAGCA GCACTCTTCC 
TCCTCCTCAG AAAAGCAGCG CTTCTACTCC TGTGATCGCT ATGGCAGCCG GGACCGATCC 
CAGCCCAAGT CTGCTGATCA GAGCAGGCCT ACTTCACCCA ATGGTGGGCC TGAGCAAGGT 
CCCCACAGAC AGGGCAGTGG TTCGGTTAAC GGGAGCCCCT TGCTGTCGAC ATCTGGTGCT 
AGTACCCCCT GCCGGGGTCG GAGGCAGCTC CCGCAGACCC CCCTGACCCC ACGCCCCAGC 
ATCACTTACA AGACCGCCAA CTCCTCGCCC GTACACTTCA GCACGTCGCC GGGCGGCCTC 
CCGCCCTTCT CCCCGGGCCG CCTGAGCCGC GGGCTGTCTG AGCACAATGC ACTGCTGCGT 
GGGGACCAGC AGCCGCCCCC GGCCGCGGTT GCCCGCATCG GCTCCGACCC TTACCTGGGC 
CACCGCGACG CCGCCGACAG CCCCATAGGC GCAGCGCCCG AGGACACGCT CACCTTCGAG 
GAAGCCGTGG CCACCAACTC GGGGCGTTCC TCCAGGACTT CTTACGTCTC CTCCTTGACC 
TCCCAGTCTC ACCAAGCACG CCGGGTGCCC AATGGCTACC ATTACACGCT GGGGCTCAAC 
ACGGGGCCCG GCACTGGCAC CAGGGGACGG AGCTACTACC ACGAAGCCGA CGAGGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134759.1
CDS	424..7392
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279273.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279273.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCGAGTT TCATGGGAGG AACCAAAGGC 
AAGGACATCA ACACGATCAA GTCGCTGCGG GTTCTGCGGG TCCTACGACC ACTGAAGACC 
ATTAAACGGC TGCCAAAACT AAAGGCTGTC TTTGACTGCG TGGTGAATTC CCTGAAGAAC 
GTCCTCAACA TCCTCATTGT CTACATGCTC TTCATGTTCA TCTTTGCTGT CATTGCTGTG 
CAGCTCTTCA AAGGCAGATT CTTCTACTGC ACTGATGAGT CGAAAGAGCT GGAGAAGGAT 
TGCAGGGGAC AATACCTTGA CTATGAAAAA AATGAGGTGG AGGCGCAACC CAGGGAATGG 
AAAAAATATG AGTTCCATTA CGACAATGTG CTGTGGGCTC TGCTGACACT TTTCACTGTC 
TCCACAGGAG AAGGGTGGCC AACTGTGCTG AAGCACTCAG TGGATGCTAC CTACGAGGAA 
CAGGGTCCCA GCCCTGGCTA CCGAATGGAA ATGTCTATCT TTTACGTGGT GTATTTTGTT 
GTCTTCCCTT TTTTCTTTGT GAACATCTTT GTGGCGTTAA TCATAATCAC CTTCCAAGAG 
CAAGGAGATA AAGTGATGTC AGAATGCAGC CTAGAGAAAA ATGAGAGGGC CTGCATTGAC 
TTCGCCATAA GTGCCAAGCC ACTGACCCGC TACATGCCTC AGAACAAGCA GTCATTTCAG 
TACAAGATGT GGAAATTTGT GGTGTCCCCG CCCTTTGAGT ATTTTATCAT GGTCATGATT 
GCACTCAATA CTATTGTTCT CATGATGAAG TTCTATGATG CTCCAGAAGC ATATGAAGAA 
ATGTTGAAAT GCCTGAATAT TGTCTTTACA TCCATGTTTT CAATGGAGTG TGTGCTGAAG 
ATCATTGCCT TTGGTGTGCT GAATTATTTC CGAGATGCCT GGAATGTCTT TGATTTTGTA 
ACAGTGTTGG GAAGTATTAC TGATATTTTA GTAACAGAGA TTGCGGACAA CTTCATCAAC 
CTCAGCTTCC TGCGGCTCTT CAGGGCAGCA AGACTTATCA AGCTCCTTCG CCAGGGTTAC 
ACTATCCGCA TCTTGCTCTG GACGTTTGTG CAGTCATTTA AGGCCTTGCC TTATGTGTGT 
CTTCTCATTG CAATGCTCTT CTTCATCTAT GCCATTATTG GCATGCAGGT ATTTGGAAAC 
ATTGCACTAA ATGACGAAAC CTCCATCAAT CGGCACAATA ATTTCCGTAC CTTCCTTCAA 
GCCTTGATGC TTCTGTTCAG GAGTGCCACG GGGGAGGCCT GGCACGAGAT CATGCTGTCA 
TGCCTGAGCA ACAGGGCCTG CGACCCACTC TCCGGCCTCA CCAAGAACGA GTGCGGCAGC 
GAGTTCGCCT ACTTCTACTT CGTGTCATTC ATCTTCCTCT GCTCCTTCCT GATGTTGAAC 
CTATTTGTGG CTGTAATCAT GGACAACTTT GAATACTTGA CAAGAGACTC CTCCATCCTT 
GGGCCCCATC ATTTGGATGA GTTTGTCCGT GTCTGGGCTG AATACGACCC AGCTGCCTGC 
GGGCGCATCA GTTACACAGA CATGTATGAG ATGCTGAGAC ACATGTCCCC ACCTCTAGGC 
CTTGGAAAGA AATGCCCTGC TCGCGTTGCC TATAAGCGCC TGGTGCGGAT GAACATGCCC 
ATCTCACCCG AGGATCTGAC AGTCCACTTC ACCTCCACGC TGATGGCCCT GATCCGCACT 
GCTCTGGAAA TCAAGCTCGC CTCAGGGGGT GTTAAGCAGC ACCAGTGTGA TGCTGAGCTG 
AGAAAGGAGA TATCTCTAGT TTGGCCCAAC CTGTCCCAGA AGACACTGGA TTTGCTGGTG 
CCTCCTCACA AACCTGATGA GATGACTGTG GGGAAGGTCT ACGCAGCGCT CATGATATTT 
GACTTCTACA AACAGAACAA GAACAGCAGG GAACAAGTCC ACCAGCCTCC TGGGGGCCTG 
TGCCAGCCCG GACCAGTGTC ACTCTTCCAC CCCTTGAAGG CCACTTTGGA GCAAACCCAG 
CCCTCGGCAT TCAGCAGTGC GAAGGCATTC CTGCGCCAGA AAAGCTCGGC CTCACTCAAC 
AATGGAGGCA CATTGCCAGC CCCAGAGGGT GGGATCAAAG AGTCCAGTTC GTGGGGGACC 
CAACGGACCC AGGACGTGTT TTATGAAACC AGGACACCAG CTTTTGAACG AGGCCACTCA 
GAGGAAATCC CCATTGAACG GGTGGTGGAA ATGAGGGAAA TCAGCCCCAC ACTTGCCAAT 
GGAGAGCACC AACCAGGGCT GGAGAGCCAG GGTCGGGCGG CTTCCATGCC GCGCCTGGCT 
GCTGAAACGC AGCCCATCCC GGACACAAGC CCCATGAAGC GCTCCATCTC CACGCTGACC 
CCGCAGCGTC CTCACCCCAT GCACCTCTAT GAGTACTCCC TGGAGCGCGT GCCCACGGAC 
CAGGTGCATC ACCACCACCA CCACCGCTGC CACCGGCGGA AAGAGAAGAA GCAGAAGTCC 
TTGGACAGGA CCACACATCA GCTGGCCGAT GGGGAAGCTG TGGCCCAGTC TGGTGAATCT 
TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA CGGTCCCAAG AGAGGAAGCA GCACTCTTCC 
TCCTCCTCAG AAAAGCAGCG CTTCTACTCC TGTGATCGCT ATGGCAGCCG GGACCGATCC 
CAGCCCAAGT CTGCTGATCA GAGCAGGCCT ACTTCACCCA ATGGTGGGCC TGAGCAAGGT 
CCCCACAGAC AGGGCAGTGG TTCGGTTAAC GGGAGCCCCT TGCTGTCGAC ATCTGGTGCT 
AGTACCCCCT GCCGGGGTCG GAGGCAGCTC CCGCAGACCC CCCTGACCCC ACGCCCCAGC 
ATCACTTACA AGACCGCCAA CTCCTCGCCC GTACACTTCA GCACGTCGCC GGGCGGCCTC 
CCGCCCTTCT CCCCGGGCCG CCTGAGCCGC GGGCTGTCTG AGCACAATGC ACTGCTGCGT 
GGGGACCAGC AGCCGCCCCC GGCCGCGGTT GCCCGCATCG GCTCCGACCC TTACCTGGGC 
CACCGCGACG CCGCCGACAG CCCCATAGGC GCAGCGCCCG AGGACACGCT CACCTTCGAG 
GAAGCCGTGG CCACCAACTC GGGGCGTTCC TCCAGGACTT CTTACGTCTC CTCCTTGACC 
TCCCAGTCTC ACCAAGCACG CCGGGTGCCC AATGGCTACC ATTACACGCT GGGGCTCAAC 
ACGGGGCCCG GCACTGGCAC CAGGGGACGG AGCTACTACC ACGAAGCCGA CGAGGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279274.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134760.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X15, mRNA.

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CloneID	OGa43300
Clone ID Related Accession (Same CDS sequence)	XM_015279274.1
Accession Version	XM_015279274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCCAA GCAGCAGAAC 
TCCTCCAAAT CCAAGTCGGT TTGGGAGCAA CGAACCAGCC AGATCCGGAT GCACAACTTC 
CGAGCCAGCT GCGAGGCTCT GTACAACGAA CTGGACCCCG AGGAGCGGGT GCGCTACGCC 
ACGACCCTAC ACATCCGTCC AGACATGAAG ACCCACTTGG ATCGGCCACT GGTGGTAGAG 
CCAAGGGGTG AAGGGAGGAA CAACATCAGT AAGCTGAGCC CTGTGGATGT GCAGGAGGTG 
GAGCAGACCA AAGTCAGCTC TACCGACGGA GCAGAAGCTC CGCGGAAGCA TCACCGGCAT 
CGGGATAAGG AGAAACTGGG AGAGCAAGAG AAGGGTGATG TGACCAAAGA TGAGAATGGG 
GAATCTGGCA TCAACAATAA GGAGGAGAGG CACAGGCAGC ACAGAAGCCG TAGCAAGGAG 
GTGGAAGGGG GCAGCAAGGA AGGGAAAAGT GACCGCAGCA GGGGCCAGGA AGGAGGGAAG 
AGGCACCATC GCCGGGGGTC GGTGGAAGAA GGCGTTGAGA AGGAGCACCG GAGGCACCGC 
ACGCACCGGC ACTCTGCAGA ACGGCAAGGC AAGGAGGGCA ATGGGACCAT CAACGGGGCC 
AGGAGTGAGC GGCGGACCCG CCACCGTGGA GGGTCGAGGT CTGGGAACCG GGAAGGAGAG 
CCTGGGTCCA AGGGCGAGAA TGGAGAGGAG CCTCACAGAC GGCACCGGTT CAGGAGCAGG 
GCGCTGTCGA CGTACGATTC GGTGGAGAAG GAGAACAGGG AGAAGGAAGG GGAGACTGCT 
GAGAAGGAGC ACCAGAATCA TCAGCCCAAG GAGAATCAGT GTGAGATTGA GGCCAGTGGG 
AGTGTGAGCA TCCCGGTGCA CACCCTTCCC AGCACCTATC TGCAGAAAGT GCCAGAGCAG 
CCTGAAGATG CTGACAACCA GAAGAACGTG ACACGCATGA TTCAGCCGCC TCTGGACAAA 
ACCACCACTG TGAACATCCC CGTCACCATC ACCGCCCCGC CAGGAGAAAC CACCGTCATA 
CCTATGAACA ACGTTGAGTT CGAAAGTAAA ACAGAAGAGA AGAAGGACGT TGATGACTTG 
ACGAAAAATG GGCCTAAACC AATCTTGCCT TACAGTTCCA TGTTCATCCT AAGTCCTACG 
AACCCGATCC GGCGTCTGTT CCACTACATC GTCAACCTGC GCTACTTCGA GATGGTCATC 
CTCATCGTCA TCGCTCTCAG CAGCATTGCC CTGGCAGCAG AAGACCCCGT CCAAGCAGAA 
TCACCAAGGA ACGATGCTCT GAAGTACTTG GATTATATAT TCACGGGTGT CTTTACCTTC 
GAGATGGTGA TCAAGATGAT TGACCTGGGG CTGTTACTTC ACCCTGGCTC CTATTTCCGT 
GACCTGTGGA ACATCTTGGA CTTCATTGTG GTCAGTGGGG CCTTGGTGGC ATTTGCTTTC 
TCAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTACA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACACGGACA ACTTCATCAA CCTCAGCTTC CTGCGGCTCT TCAGGGCAGC AAGACTTATC 
AAGCTCCTTC GCCAGGGTTA CACTATCCGC ATCTTGCTCT GGACGTTTGT GCAGTCATTT 
AAGGCCTTGC CTTATGTGTG TCTTCTCATT GCAATGCTCT TCTTCATCTA TGCCATTATT 
GGCATGCAGG TATTTGGAAA CATTGCACTA AATGACGAAA CCTCCATCAA TCGGCACAAT 
AATTTCCGTA CCTTCCTTCA AGCCTTGATG CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC ATGCCTGAGC AACAGGGCCT GCGACCCACT CTCCGGCCTC 
ACCAAGAACG AGTGCGGCAG CGAGTTCGCC TACTTCTACT TCGTGTCATT CATCTTCCTC 
TGCTCCTTCC TGATGTTGAA CCTATTTGTG GCTGTAATCA TGGACAACTT TGAATACTTG 
ACAAGAGACT CCTCCATCCT TGGGCCCCAT CATTTGGATG AGTTTGTCCG TGTCTGGGCT 
GAATACGACC CAGCTGCCTG TTGCCGGATT CATTATAAGG ATATGTACAA TTTGTTACGT 
GTAATTGCTC CACCCCTGGG CTTAGGAAAG AAGTGCCCTC ATAGGGTGGC ATACAAGCGC 
CTGGTGCGGA TGAACATGCC CATCTCACCC GAGGATCTGA CAGTCCACTT CACCTCCACG 
CTGATGGCCC TGATCCGCAC TGCTCTGGAA ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAGGAG ATATCTCTAG TTTGGCCCAA CCTGTCCCAG 
AAGACACTGG ATTTGCTGGT GCCTCCTCAC AAACCTGATG AGATGACTGT GGGGAAGGTC 
TACGCAGCGC TCATGATATT TGACTTCTAC AAACAGAACA AGAACAGCAG GGAACAAGTC 
CACCAGCCTC CTGGGGGCCT GTGCCAGCCC GGACCAGTGT CACTCTTCCA CCCCTTGAAG 
GCCACTTTGG AGCAAACCCA GCCCTCGGCA TTCAGCAGTG CGAAGGCATT CCTGCGCCAG 
AAAAGCTCGG CCTCACTCAA CAATGGAGGC ACATTGCCAG CCCCAGAGGG TGGGATCAAA 
GAGTCCAGTT CGTGGGGGAC CCAACGGACC CAGGACGTGT TTTATGAAAC CAGGACACCA 
GCTTTTGAAC GAGGCCACTC AGAGGAAATC CCCATTGAAC GGGTGGTGGA AATGAGGGAA 
ATCAGCCCCA CACTTGCCAA TGGAGAGCAC CAACCAGGGC TGGAGAGCCA GGGTCGGGCG 
GCTTCCATGC CGCGCCTGGC TGCTGAAACG CAGAGGAGCA AAGCACGGTC ACCTGGGAGT 
TACCTAGCAC CCATCCCGGA CACAAGCCCC ATGAAGCGCT CCATCTCCAC GCTGACCCCG 
CAGCGTCCTC ACCCCATGCA CCTCTATGAG TACTCCCTGG AGCGCGTGCC CACGGACCAG 
GTGCATCACC ACCACCACCA CCGCTGCCAC CGGCGGAAAG AGAAGAAGCA GAAGTCCTTG 
GACAGGACCA CACATCAGCT GGCCGATGGG GAAGCTGTGG CCCAGTCTGG TGAATCTTCT 
TCCAAGGACA AGAAGCAAGA GCGTGGACGG TCCCAAGAGA GGAAGCAGCA CTCTTCCTCC 
TCCTCAGAAA AGCAGCGCTT CTACTCCTGT GATCGCTATG GCAGCCGGGA CCGATCCCAG 
CCCAAGTCTG CTGATCAGAG CAGGCCTACT TCACCCAATG GTGGGCCTGA GCAAGGTCCC 
CACAGACAGG GCAGTGGTTC GGTTAACGGG AGCCCCTTGC TGTCGACATC TGGTGCTAGT 
ACCCCCTGCC GGGGTCGGAG GCAGCTCCCG CAGACCCCCC TGACCCCACG CCCCAGCATC 
ACTTACAAGA CCGCCAACTC CTCGCCCGTA CACTTCAGCA CGTCGCCGGG CGGCCTCCCG 
CCCTTCTCCC CGGGCCGCCT GAGCCGCGGG CTGTCTGAGC ACAATGCACT GCTGCGTGGG 
GACCAGCAGC CGCCCCCGGC CGCGGTTGCC CGCATCGGCT CCGACCCTTA CCTGGGCCAC 
CGCGACGCCG CCGACAGCCC CATAGGCGCA GCGCCCGAGG ACACGCTCAC CTTCGAGGAA 
GCCGTGGCCA CCAACTCGGG GCGTTCCTCC AGGACTTCTT ACGTCTCCTC CTTGACCTCC 
CAGTCTCACC AAGCACGCCG GGTGCCCAAT GGCTACCATT ACACGCTGGG GCTCAACACG 
GGGCCCGGCA CTGGCACCAG GGGACGGAGC TACTACCACG AAGCCGACGA GGATGACTGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134760.1
CDS	427..7392
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279274.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279274.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCCAA GCAGCAGAAC 
TCCTCCAAAT CCAAGTCGGT TTGGGAGCAA CGAACCAGCC AGATCCGGAT GCACAACTTC 
CGAGCCAGCT GCGAGGCTCT GTACAACGAA CTGGACCCCG AGGAGCGGGT GCGCTACGCC 
ACGACCCTAC ACATCCGTCC AGACATGAAG ACCCACTTGG ATCGGCCACT GGTGGTAGAG 
CCAAGGGGTG AAGGGAGGAA CAACATCAGT AAGCTGAGCC CTGTGGATGT GCAGGAGGTG 
GAGCAGACCA AAGTCAGCTC TACCGACGGA GCAGAAGCTC CGCGGAAGCA TCACCGGCAT 
CGGGATAAGG AGAAACTGGG AGAGCAAGAG AAGGGTGATG TGACCAAAGA TGAGAATGGG 
GAATCTGGCA TCAACAATAA GGAGGAGAGG CACAGGCAGC ACAGAAGCCG TAGCAAGGAG 
GTGGAAGGGG GCAGCAAGGA AGGGAAAAGT GACCGCAGCA GGGGCCAGGA AGGAGGGAAG 
AGGCACCATC GCCGGGGGTC GGTGGAAGAA GGCGTTGAGA AGGAGCACCG GAGGCACCGC 
ACGCACCGGC ACTCTGCAGA ACGGCAAGGC AAGGAGGGCA ATGGGACCAT CAACGGGGCC 
AGGAGTGAGC GGCGGACCCG CCACCGTGGA GGGTCGAGGT CTGGGAACCG GGAAGGAGAG 
CCTGGGTCCA AGGGCGAGAA TGGAGAGGAG CCTCACAGAC GGCACCGGTT CAGGAGCAGG 
GCGCTGTCGA CGTACGATTC GGTGGAGAAG GAGAACAGGG AGAAGGAAGG GGAGACTGCT 
GAGAAGGAGC ACCAGAATCA TCAGCCCAAG GAGAATCAGT GTGAGATTGA GGCCAGTGGG 
AGTGTGAGCA TCCCGGTGCA CACCCTTCCC AGCACCTATC TGCAGAAAGT GCCAGAGCAG 
CCTGAAGATG CTGACAACCA GAAGAACGTG ACACGCATGA TTCAGCCGCC TCTGGACAAA 
ACCACCACTG TGAACATCCC CGTCACCATC ACCGCCCCGC CAGGAGAAAC CACCGTCATA 
CCTATGAACA ACGTTGAGTT CGAAAGTAAA ACAGAAGAGA AGAAGGACGT TGATGACTTG 
ACGAAAAATG GGCCTAAACC AATCTTGCCT TACAGTTCCA TGTTCATCCT AAGTCCTACG 
AACCCGATCC GGCGTCTGTT CCACTACATC GTCAACCTGC GCTACTTCGA GATGGTCATC 
CTCATCGTCA TCGCTCTCAG CAGCATTGCC CTGGCAGCAG AAGACCCCGT CCAAGCAGAA 
TCACCAAGGA ACGATGCTCT GAAGTACTTG GATTATATAT TCACGGGTGT CTTTACCTTC 
GAGATGGTGA TCAAGATGAT TGACCTGGGG CTGTTACTTC ACCCTGGCTC CTATTTCCGT 
GACCTGTGGA ACATCTTGGA CTTCATTGTG GTCAGTGGGG CCTTGGTGGC ATTTGCTTTC 
TCAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTACA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACACGGACA ACTTCATCAA CCTCAGCTTC CTGCGGCTCT TCAGGGCAGC AAGACTTATC 
AAGCTCCTTC GCCAGGGTTA CACTATCCGC ATCTTGCTCT GGACGTTTGT GCAGTCATTT 
AAGGCCTTGC CTTATGTGTG TCTTCTCATT GCAATGCTCT TCTTCATCTA TGCCATTATT 
GGCATGCAGG TATTTGGAAA CATTGCACTA AATGACGAAA CCTCCATCAA TCGGCACAAT 
AATTTCCGTA CCTTCCTTCA AGCCTTGATG CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC ATGCCTGAGC AACAGGGCCT GCGACCCACT CTCCGGCCTC 
ACCAAGAACG AGTGCGGCAG CGAGTTCGCC TACTTCTACT TCGTGTCATT CATCTTCCTC 
TGCTCCTTCC TGATGTTGAA CCTATTTGTG GCTGTAATCA TGGACAACTT TGAATACTTG 
ACAAGAGACT CCTCCATCCT TGGGCCCCAT CATTTGGATG AGTTTGTCCG TGTCTGGGCT 
GAATACGACC CAGCTGCCTG TTGCCGGATT CATTATAAGG ATATGTACAA TTTGTTACGT 
GTAATTGCTC CACCCCTGGG CTTAGGAAAG AAGTGCCCTC ATAGGGTGGC ATACAAGCGC 
CTGGTGCGGA TGAACATGCC CATCTCACCC GAGGATCTGA CAGTCCACTT CACCTCCACG 
CTGATGGCCC TGATCCGCAC TGCTCTGGAA ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAGGAG ATATCTCTAG TTTGGCCCAA CCTGTCCCAG 
AAGACACTGG ATTTGCTGGT GCCTCCTCAC AAACCTGATG AGATGACTGT GGGGAAGGTC 
TACGCAGCGC TCATGATATT TGACTTCTAC AAACAGAACA AGAACAGCAG GGAACAAGTC 
CACCAGCCTC CTGGGGGCCT GTGCCAGCCC GGACCAGTGT CACTCTTCCA CCCCTTGAAG 
GCCACTTTGG AGCAAACCCA GCCCTCGGCA TTCAGCAGTG CGAAGGCATT CCTGCGCCAG 
AAAAGCTCGG CCTCACTCAA CAATGGAGGC ACATTGCCAG CCCCAGAGGG TGGGATCAAA 
GAGTCCAGTT CGTGGGGGAC CCAACGGACC CAGGACGTGT TTTATGAAAC CAGGACACCA 
GCTTTTGAAC GAGGCCACTC AGAGGAAATC CCCATTGAAC GGGTGGTGGA AATGAGGGAA 
ATCAGCCCCA CACTTGCCAA TGGAGAGCAC CAACCAGGGC TGGAGAGCCA GGGTCGGGCG 
GCTTCCATGC CGCGCCTGGC TGCTGAAACG CAGAGGAGCA AAGCACGGTC ACCTGGGAGT 
TACCTAGCAC CCATCCCGGA CACAAGCCCC ATGAAGCGCT CCATCTCCAC GCTGACCCCG 
CAGCGTCCTC ACCCCATGCA CCTCTATGAG TACTCCCTGG AGCGCGTGCC CACGGACCAG 
GTGCATCACC ACCACCACCA CCGCTGCCAC CGGCGGAAAG AGAAGAAGCA GAAGTCCTTG 
GACAGGACCA CACATCAGCT GGCCGATGGG GAAGCTGTGG CCCAGTCTGG TGAATCTTCT 
TCCAAGGACA AGAAGCAAGA GCGTGGACGG TCCCAAGAGA GGAAGCAGCA CTCTTCCTCC 
TCCTCAGAAA AGCAGCGCTT CTACTCCTGT GATCGCTATG GCAGCCGGGA CCGATCCCAG 
CCCAAGTCTG CTGATCAGAG CAGGCCTACT TCACCCAATG GTGGGCCTGA GCAAGGTCCC 
CACAGACAGG GCAGTGGTTC GGTTAACGGG AGCCCCTTGC TGTCGACATC TGGTGCTAGT 
ACCCCCTGCC GGGGTCGGAG GCAGCTCCCG CAGACCCCCC TGACCCCACG CCCCAGCATC 
ACTTACAAGA CCGCCAACTC CTCGCCCGTA CACTTCAGCA CGTCGCCGGG CGGCCTCCCG 
CCCTTCTCCC CGGGCCGCCT GAGCCGCGGG CTGTCTGAGC ACAATGCACT GCTGCGTGGG 
GACCAGCAGC CGCCCCCGGC CGCGGTTGCC CGCATCGGCT CCGACCCTTA CCTGGGCCAC 
CGCGACGCCG CCGACAGCCC CATAGGCGCA GCGCCCGAGG ACACGCTCAC CTTCGAGGAA 
GCCGTGGCCA CCAACTCGGG GCGTTCCTCC AGGACTTCTT ACGTCTCCTC CTTGACCTCC 
CAGTCTCACC AAGCACGCCG GGTGCCCAAT GGCTACCATT ACACGCTGGG GCTCAACACG 
GGGCCCGGCA CTGGCACCAG GGGACGGAGC TACTACCACG AAGCCGACGA GGATGACTGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279275.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134761.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X16, mRNA.

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CloneID	OGa43301
Clone ID Related Accession (Same CDS sequence)	XM_015279275.1
Accession Version	XM_015279275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ACATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGAGTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGCGGGCGC ATCAGTTACA CAGACATGTA TGAGATGCTG 
AGACACATGT CCCCACCTCT AGGCCTTGGA AAGAAATGCC CTGCTCGCGT TGCCTATAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGTGGT TCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134761.1
CDS	427..7014
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279275.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279275.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCTTTTG TAAAGCAAAC TCGAGGTACT 
GTATCTCGCA GCTCGTCAGT CTCCAGCGTA AATTCACCCA AGCAGCAGAA CTCCTCCAAA 
TCCAAGTCGG TTTGGGAGCA ACGAACCAGC CAGATCCGGA TGCACAACTT CCGAGCCAGC 
TGCGAGGCTC TGTACAACGA ACTGGACCCC GAGGAGCGGG TGCGCTACGC CACGACCCTA 
CACATCCGTC CAGACATGAA GACCCACTTG GATCGGCCAC TGGTGGTAGA GCCAAGGGGT 
GAAGGGAGGA ACAACATCAG TAAGCTGAGC CCTGTGGATG TGCAGGAGGT GGAGCAGACC 
AAAGTCAGCT CTACCGACGG AGCAGAAGCT CCGCGGAAGC ATCACCGGCA TCGGGATAAG 
GAGAAACTGG GAGAGCAAGA GAAGGGTGAT GTGACCAAAG ATGAGAATGG GGAATCTGGC 
ATCAACAATA AGGAGGAGAG GCACAGGCAG CACAGAAGCC GTAGCAAGGA GGTGGAAGGG 
GGCAGCAAGG AAGGGAAAAG TGACCGCAGC AGGGGCCAGG AAGGAGGGAA GAGGCACCAT 
CGCCGGGGGT CGGTGGAAGA AGGCGTTGAG AAGGAGCACC GGAGGCACCG CACGCACCGG 
CACTCTGCAG AACGGCAAGG CAAGGAGGGC AATGGGACCA TCAACGGGGC CAGGAGTGAG 
CGGCGGACCC GCCACCGTGG AGGGTCGAGG TCTGGGAACC GGGAAGGAGA GCCTGGGTCC 
AAGGGCGAGA ATGGAGAGGA GCCTCACAGA CGGCACCGGT TCAGGAGCAG GGCGCTGTCG 
ACGTACGATT CGGTGGAGAA GGAGAACAGG GAGAAGGAAG GGGAGACTGC TGAGAAGGAG 
CACCAGAATC ATCAGCCCAA GGAGAATCAG TGTGAGATTG AGGCCAGTGG GAGTGTGAGC 
ATCCCGGTGC ACACCCTTCC CAGCACCTAT CTGCAGAAAG TGCCAGAGCA GCCTGAAGAT 
GCTGACAACC AGAAGAACGT GACACGCATG ATTCAGCCGC CTCTGGACAA AACCACCACT 
GTGAACATCC CCGTCACCAT CACCGCCCCG CCAGGAGAAA CCACCGTCAT ACCTATGAAC 
AACGTTGAGT TCGAAAGTAA AACAGAAGAG AAGAAGGACG TTGATGACTT GACGAAAAAT 
GGGCCTAAAC CAATCTTGCC TTACAGTTCC ATGTTCATCC TAAGTCCTAC GAACCCGATC 
CGGCGTCTGT TCCACTACAT CGTCAACCTG CGCTACTTCG AGATGGTCAT CCTCATCGTC 
ATCGCTCTCA GCAGCATTGC CCTGGCAGCA GAAGACCCCG TCCAAGCAGA ATCACCAAGG 
AACGATGCTC TGAAGTACTT GGATTATATA TTCACGGGTG TCTTTACCTT CGAGATGGTG 
ATCAAGATGA TTGACCTGGG GCTGTTACTT CACCCTGGCT CCTATTTCCG TGACCTGTGG 
AACATCTTGG ACTTCATTGT GGTCAGTGGG GCCTTGGTGG CATTTGCTTT CTCGAGTTTC 
ATGGGAGGAA CCAAAGGCAA GGACATCAAC ACGATCAAGT CGCTGCGGGT TCTGCGGGTC 
CTACGACCAC TGAAGACCAT TAAACGGCTG CCAAAACTAA AGGCTGTCTT TGACTGCGTG 
GTGAATTCCC TGAAGAACGT CCTCAACATC CTCATTGTCT ACATGCTCTT CATGTTCATC 
TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA GGCAGATTCT TCTACTGCAC TGATGAGTCG 
AAAGAGCTGG AGAAGGATTG CAGGGGACAA TACCTTGACT ATGAAAAAAA TGAGGTGGAG 
GCGCAACCCA GGGAATGGAA AAAATATGAG TTCCATTACG ACAATGTGCT GTGGGCTCTG 
CTGACACTTT TCACTGTCTC CACAGGAGAA GGGTGGCCAA CTGTGCTGAA GCACTCAGTG 
GATGCTACCT ACGAGGAACA GGGTCCCAGC CCTGGCTACC GAATGGAAAT GTCTATCTTT 
TACGTGGTGT ATTTTGTTGT CTTCCCTTTT TTCTTTGTGA ACATCTTTGT GGCGTTAATC 
ATAATCACCT TCCAAGAGCA AGGAGATAAA GTGATGTCAG AATGCAGCCT AGAGAAAAAT 
GAGAGGGCCT GCATTGACTT CGCCATAAGT GCCAAGCCAC TGACCCGCTA CATGCCTCAG 
AACAAGCAGT CATTTCAGTA CAAGATGTGG AAATTTGTGG TGTCCCCGCC CTTTGAGTAT 
TTTATCATGG TCATGATTGC ACTCAATACT ATTGTTCTCA TGATGAAGTT CTATGATGCT 
CCAGAAGCAT ATGAAGAAAT GTTGAAATGC CTGAATATTG TCTTTACATC CATGTTTTCA 
ATGGAGTGTG TGCTGAAGAT CATTGCCTTT GGTGTGCTGA ATTATTTCCG AGATGCCTGG 
AATGTCTTTG ATTTTGTAAC AGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGACACGG ACAACTTCAT CAACCTCAGC TTCCTGCGGC TCTTCAGGGC AGCAAGACTT 
ATCAAGCTCC TTCGCCAGGG TTACACTATC CGCATCTTGC TCTGGACGTT TGTGCAGTCA 
TTTAAGGCCT TGCCTTATGT GTGTCTTCTC ATTGCAATGC TCTTCTTCAT CTATGCCATT 
ATTGGCATGC AGGTATTTGG AAACATTGCA CTAAATGACG AAACCTCCAT CAATCGGCAC 
AATAATTTCC GTACCTTCCT TCAAGCCTTG ATGCTTCTGT TCAGGAGTGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCATGCCTG AGCAACAGGG CCTGCGACCC ACTCTCCGGC 
CTCACCAAGA ACGAGTGCGG CAGCGAGTTC GCCTACTTCT ACTTCGTGTC ATTCATCTTC 
CTCTGCTCCT TCCTGATGTT GAACCTATTT GTGGCTGTAA TCATGGACAA CTTTGAATAC 
TTGACAAGAG ACTCCTCCAT CCTTGGGCCC CATCATTTGG ATGAGTTTGT CCGTGTCTGG 
GCTGAATACG ACCCAGCTGC CTGCGGGCGC ATCAGTTACA CAGACATGTA TGAGATGCTG 
AGACACATGT CCCCACCTCT AGGCCTTGGA AAGAAATGCC CTGCTCGCGT TGCCTATAAG 
CGCCTGGTGC GGATGAACAT GCCCATCTCA CCCGAGGATC TGACAGTCCA CTTCACCTCC 
ACGCTGATGG CCCTGATCCG CACTGCTCTG GAAATCAAGC TCGCCTCAGG GGGTGTTAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAG GAGATATCTC TAGTTTGGCC CAACCTGTCC 
CAGAAGACAC TGGATTTGCT GGTGCCTCCT CACAAACCTG ATGAGATGAC TGTGGGGAAG 
GTCTACGCAG CGCTCATGAT ATTTGACTTC TACAAACAGA ACAAGAACAG CAGGGAACAA 
GTCCACCAGC CTCCTGGGGG CCTGTGCCAG CCCGGACCAG TGTCACTCTT CCACCCCTTG 
AAGGCCACTT TGGAGCAAAC CCAGCCCTCG GCATTCAGCA GTGCGAAGGC ATTCCTGCGC 
CAGAAAAGCT CGGCCTCACT CAACAATGGA GGCACATTGC CAGCCCCAGA GGGTGGGATC 
AAAGAGTCCA GTTCGTGGGG GACCCAACGG ACCCAGGACG TGTTTTATGA AACCAGGACA 
CCAGCTTTTG AACGAGGCCA CTCAGAGGAA ATCCCCATTG AACGGGTGGT GGAAATGAGG 
GAAATCAGCC CCACACTTGC CAATGGAGAG CACCAACCAG GGCTGGAGAG CCAGGGTCGG 
GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA ACGCAGCCCA TCCCGGACAC AAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GACCCCGCAG CGTCCTCACC CCATGCACCT CTATGAGTAC 
TCCCTGGAGC GCGTGCCCAC GGACCAGGTG CATCACCACC ACCACCACCG CTGCCACCGG 
CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC AGGACCACAC ATCAGCTGGC CGATGGGGAA 
GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC AAGGACAAGA AGCAAGAGCG TGGACGGTCC 
CAAGAGAGGA AGCAGCACTC TTCCTCCTCC TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT 
CGCTATGGCA GCCGGGACCG ATCCCAGCCC AAGTCTGCTG ATCAGAGCAG GCCTACTTCA 
CCCAATGGTG GGCCTGAGCA AGGTCCCCAC AGACAGTGGT TCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279276.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134762.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X17, mRNA.

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CloneID	OGa43302
Clone ID Related Accession (Same CDS sequence)	XM_015279276.1
Accession Version	XM_015279276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG AGTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGTT GCCGGATTCA TTATAAGGAT ATGTACAATT TGTTACGTGT AATTGCTCCA 
CCCCTGGGCT TAGGAAAGAA GTGCCCTCAT AGGGTGGCAT ACAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACA GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACACTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AACAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ATGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCGGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GAGGAGCAAA GCACGGTCAC CTGGGAGTTA CCTAGCACCC 
ATCCCGGACA CAAGCCCCAT GAAGCGCTCC ATCTCCACGC TGACCCCGCA GCGTCCTCAC 
CCCATGCACC TCTATGAGTA CTCCCTGGAG CGCGTGCCCA CGGACCAGGT GCATCACCAC 
CACCACCACC GCTGCCACCG GCGGAAAGAG AAGAAGCAGA AGTCCTTGGA CAGGACCACA 
CATCAGCTGG CCGATGGGGA AGCTGTGGCC CAGTCTGGTG AATCTTCTTC CAAGGACAAG 
AAGCAAGAGC GTGGACGGTC CCAAGAGAGG AAGCAGCACT CTTCCTCCTC CTCAGAAAAG 
CAGCGCTTCT ACTCCTGTGA TCGCTATGGC AGCCGGGACC GATCCCAGCC CAAGTCTGCT 
GATCAGAGCA GGCCTACTTC ACCCAATGGT GGGCCTGAGC AAGGTCCCCA CAGACAGTGG 
TTCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134762.1
CDS	429..6977
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279276.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279276.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT GCTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAAGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAACGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTACATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG AGTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGTT GCCGGATTCA TTATAAGGAT ATGTACAATT TGTTACGTGT AATTGCTCCA 
CCCCTGGGCT TAGGAAAGAA GTGCCCTCAT AGGGTGGCAT ACAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACA GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACACTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AACAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ATGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCGGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GAGGAGCAAA GCACGGTCAC CTGGGAGTTA CCTAGCACCC 
ATCCCGGACA CAAGCCCCAT GAAGCGCTCC ATCTCCACGC TGACCCCGCA GCGTCCTCAC 
CCCATGCACC TCTATGAGTA CTCCCTGGAG CGCGTGCCCA CGGACCAGGT GCATCACCAC 
CACCACCACC GCTGCCACCG GCGGAAAGAG AAGAAGCAGA AGTCCTTGGA CAGGACCACA 
CATCAGCTGG CCGATGGGGA AGCTGTGGCC CAGTCTGGTG AATCTTCTTC CAAGGACAAG 
AAGCAAGAGC GTGGACGGTC CCAAGAGAGG AAGCAGCACT CTTCCTCCTC CTCAGAAAAG 
CAGCGCTTCT ACTCCTGTGA TCGCTATGGC AGCCGGGACC GATCCCAGCC CAAGTCTGCT 
GATCAGAGCA GGCCTACTTC ACCCAATGGT GGGCCTGAGC AAGGTCCCCA CAGACAGTGG 
TTCGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279277.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134763.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X18, mRNA.

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CloneID	OGa43303
Clone ID Related Accession (Same CDS sequence)	XM_015279277.1
Accession Version	XM_015279277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCCAAGCAGC AGAACTCCTC CAAATCCAAG TCGGTTTGGG AGCAACGAAC CAGCCAGATC 
CGGATGCACA ACTTCCGAGC CAGCTGCGAG GCTCTGTACA ACGAACTGGA CCCCGAGGAG 
CGGGTGCGCT ACGCCACGAC CCTACACATC CGTCCAGACA TGAAGACCCA CTTGGATCGG 
CCACTGGTGG TAGAGCCAAG GGGTGAAGGG AGGAACAACA TCAGTAAGCT GAGCCCTGTG 
GATGTGCAGG AGGTGGAGCA GACCAAAGTC AGCTCTACCG ACGGAGCAGA AGCTCCGCGG 
AAGCATCACC GGCATCGGGA TAAGGAGAAA CTGGGAGAGC AAGAGAAGGG TGATGTGACC 
AAAGATGAGA ATGGGGAATC TGGCATCAAC AATAAGGAGG AGAGGCACAG GCAGCACAGA 
AGCCGTAGCA AGGAGGTGGA AGGGGGCAGC AAGGAAGGGA AAAGTGACCG CAGCAGGGGC 
CAGGAAGGAG GGAAGAGGCA CCATCGCCGG GGGTCGGTGG AAGAAGGCGT TGAGAAGGAG 
CACCGGAGGC ACCGCACGCA CCGGCACTCT GCAGAACGGC AAGGCAAGGA GGGCAATGGG 
ACCATCAACG GGGCCAGGAG TGAGCGGCGG ACCCGCCACC GTGGAGGGTC GAGGTCTGGG 
AACCGGGAAG GAGAGCCTGG GTCCAAGGGC GAGAATGGAG AGGAGCCTCA CAGACGGCAC 
CGGTTCAGGA GCAGGGCGCT GTCGACGTAC GATTCGGTGG AGAAGGAGAA CAGGGAGAAG 
GAAGGGGAGA CTGCTGAGAA GGAGCACCAG AATCATCAGC CCAAGGAGAA TCAGTGTGAG 
ATTGAGGCCA GTGGGAGTGT GAGCATCCCG GTGCACACCC TTCCCAGCAC CTATCTGCAG 
AAAGTGCCAG AGCAGCCTGA AGATGCTGAC AACCAGAAGA ACGTGACACG CATGATTCAG 
CCGCCTCTGG ACAAAACCAC CACTGTGAAC ATCCCCGTCA CCATCACCGC CCCGCCAGGA 
GAAACCACCG TCATACCTAT GAACAACGTT GAGTTCGAAA GTAAAACAGA AGAGAAGAAG 
GACGTTGATG ACTTGACGAA AAATGGGCCT AAACCAATCT TGCCTTACAG TTCCATGTTC 
ATCCTAAGTC CTACGAACCC GATCCGGCGT CTGTTCCACT ACATCGTCAA CCTGCGCTAC 
TTCGAGATGG TCATCCTCAT CGTCATCGCT CTCAGCAGCA TTGCCCTGGC AGCAGAAGAC 
CCCGTCCAAG CAGAATCACC AAGGAACGAT GCTCTGAAGT ACTTGGATTA TATATTCACG 
GGTGTCTTTA CCTTCGAGAT GGTGATCAAG ATGATTGACC TGGGGCTGTT ACTTCACCCT 
GGCTCCTATT TCCGTGACCT GTGGAACATC TTGGACTTCA TTGTGGTCAG TGGGGCCTTG 
GTGGCATTTG CTTTCTCAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTACATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGAGT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGTTGCC GGATTCATTA TAAGGATATG 
TACAATTTGT TACGTGTAAT TGCTCCACCC CTGGGCTTAG GAAAGAAGTG CCCTCATAGG 
GTGGCATACA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGAG GAGCAAAGCA 
CGGTCACCTG GGAGTTACCT AGCACCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGTGGTTC GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134763.1
CDS	429..6944
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279277.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279277.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGG TCTCCTTTTG CCCGTGCCAG TTTGAAAAGT 
GGGAAGAATG AGAGCTCATC CTACTTCAGG AGGAAAGAGA AAATGTTCCG CTTCTTCATC 
CGGCGCATGG TGAAAGCCCA GAGCTTCTAC TGGATTGTGC TCTGCGTGGT TGCCCTCAAC 
ACGCTCTGCG TCGCGATGGT GCACTATGAT CAGCCAGAGA AACTCACCAC CGCGCTGTAT 
TTCGCCGAGT TTGTTTTTCT GGGTCTGTTC CTTACTGAGA TGTCTTTGAA GATGTATGGA 
CTGGGGCCAA GAAATTACTT TCATTCCTCA TTCAACTGCT TTGACTTTGG GGTCATTGTG 
GGGAGCATCT TTGAAGTCAT CTGGGCTGCA GTGAAACCTG GTACCTCGTT TGGCATCAGC 
GTGCTGAGAG CTCTGCGGCT GCTGAGGATT TTCAAAGTCA CCAAATACTG GAACTCCCTG 
AGGAACCTCG TGGTCTCCTT GCTGAACTCC ATGAAGTCCA TCATCAGTTT GCTCTTTCTG 
CTGTTCCTCT TCATTGTGGT GTTTGCTCTG CTGGGGATGC AGCTCTTTGG AGGGCAGTTC 
AATTTTAGAG ATGAAACACC AACCACGAAC TTCGATACTT TTCCAGCTGC CATCCTCACG 
GTGTTCCAGA TCCTGACTGG GGAGGACTGG AACGCGGTGA TGTACCATGG CATAGAGTCG 
CAGGGCGGTG TCCACTCGGG GATGTTCTCC TCCATTTACT TCATCGTCCT GACGTTGTTT 
GGTAACTATA CACTCCTGAA CGTCTTCTTG GCCATCGCCG TTGACAATCT GGCCAATGCC 
CAAGAGCTGA CCAAGGACGA GGAGGAGATG GAGGAAGCCA CCAATCAGAA GCTCGCCCTG 
CAGAAGGCCA AGGAGGTAGC AGAAGTCAGC CCCATGTCTG CAGCAAACAT TTCCATAGCC 
GCCAAGCAGC AGAACTCCTC CAAATCCAAG TCGGTTTGGG AGCAACGAAC CAGCCAGATC 
CGGATGCACA ACTTCCGAGC CAGCTGCGAG GCTCTGTACA ACGAACTGGA CCCCGAGGAG 
CGGGTGCGCT ACGCCACGAC CCTACACATC CGTCCAGACA TGAAGACCCA CTTGGATCGG 
CCACTGGTGG TAGAGCCAAG GGGTGAAGGG AGGAACAACA TCAGTAAGCT GAGCCCTGTG 
GATGTGCAGG AGGTGGAGCA GACCAAAGTC AGCTCTACCG ACGGAGCAGA AGCTCCGCGG 
AAGCATCACC GGCATCGGGA TAAGGAGAAA CTGGGAGAGC AAGAGAAGGG TGATGTGACC 
AAAGATGAGA ATGGGGAATC TGGCATCAAC AATAAGGAGG AGAGGCACAG GCAGCACAGA 
AGCCGTAGCA AGGAGGTGGA AGGGGGCAGC AAGGAAGGGA AAAGTGACCG CAGCAGGGGC 
CAGGAAGGAG GGAAGAGGCA CCATCGCCGG GGGTCGGTGG AAGAAGGCGT TGAGAAGGAG 
CACCGGAGGC ACCGCACGCA CCGGCACTCT GCAGAACGGC AAGGCAAGGA GGGCAATGGG 
ACCATCAACG GGGCCAGGAG TGAGCGGCGG ACCCGCCACC GTGGAGGGTC GAGGTCTGGG 
AACCGGGAAG GAGAGCCTGG GTCCAAGGGC GAGAATGGAG AGGAGCCTCA CAGACGGCAC 
CGGTTCAGGA GCAGGGCGCT GTCGACGTAC GATTCGGTGG AGAAGGAGAA CAGGGAGAAG 
GAAGGGGAGA CTGCTGAGAA GGAGCACCAG AATCATCAGC CCAAGGAGAA TCAGTGTGAG 
ATTGAGGCCA GTGGGAGTGT GAGCATCCCG GTGCACACCC TTCCCAGCAC CTATCTGCAG 
AAAGTGCCAG AGCAGCCTGA AGATGCTGAC AACCAGAAGA ACGTGACACG CATGATTCAG 
CCGCCTCTGG ACAAAACCAC CACTGTGAAC ATCCCCGTCA CCATCACCGC CCCGCCAGGA 
GAAACCACCG TCATACCTAT GAACAACGTT GAGTTCGAAA GTAAAACAGA AGAGAAGAAG 
GACGTTGATG ACTTGACGAA AAATGGGCCT AAACCAATCT TGCCTTACAG TTCCATGTTC 
ATCCTAAGTC CTACGAACCC GATCCGGCGT CTGTTCCACT ACATCGTCAA CCTGCGCTAC 
TTCGAGATGG TCATCCTCAT CGTCATCGCT CTCAGCAGCA TTGCCCTGGC AGCAGAAGAC 
CCCGTCCAAG CAGAATCACC AAGGAACGAT GCTCTGAAGT ACTTGGATTA TATATTCACG 
GGTGTCTTTA CCTTCGAGAT GGTGATCAAG ATGATTGACC TGGGGCTGTT ACTTCACCCT 
GGCTCCTATT TCCGTGACCT GTGGAACATC TTGGACTTCA TTGTGGTCAG TGGGGCCTTG 
GTGGCATTTG CTTTCTCAGG AACCAAAGGC AAGGACATCA ACACGATCAA GTCGCTGCGG 
GTTCTGCGGG TCCTACGACC ACTGAAGACC ATTAAACGGC TGCCAAAACT AAAGGCTGTC 
TTTGACTGCG TGGTGAATTC CCTGAAGAAC GTCCTCAACA TCCTCATTGT CTACATGCTC 
TTCATGTTCA TCTTTGCTGT CATTGCTGTG CAGCTCTTCA AAGGCAGATT CTTCTACTGC 
ACTGATGAGT CGAAAGAGCT GGAGAAGGAT TGCAGGGGAC AATACCTTGA CTATGAAAAA 
AATGAGGTGG AGGCGCAACC CAGGGAATGG AAAAAATATG AGTTCCATTA CGACAATGTG 
CTGTGGGCTC TGCTGACACT TTTCACTGTC TCCACAGGAG AAGGGTGGCC AACTGTGCTG 
AAGCACTCAG TGGATGCTAC CTACGAGGAA CAGGGTCCCA GCCCTGGCTA CCGAATGGAA 
ATGTCTATCT TTTACGTGGT GTATTTTGTT GTCTTCCCTT TTTTCTTTGT GAACATCTTT 
GTGGCGTTAA TCATAATCAC CTTCCAAGAG CAAGGAGATA AAGTGATGTC AGAATGCAGC 
CTAGAGAAAA ATGAGAGGGC CTGCATTGAC TTCGCCATAA GTGCCAAGCC ACTGACCCGC 
TACATGCCTC AGAACAAGCA GTCATTTCAG TACAAGATGT GGAAATTTGT GGTGTCCCCG 
CCCTTTGAGT ATTTTATCAT GGTCATGATT GCACTCAATA CTATTGTTCT CATGATGAAG 
TTCTATGATG CTCCAGAAGC ATATGAAGAA ATGTTGAAAT GCCTGAATAT TGTCTTTACA 
TCCATGTTTT CAATGGAGTG TGTGCTGAAG ATCATTGCCT TTGGTGTGCT GAATTATTTC 
CGAGATGCCT GGAATGTCTT TGATTTTGTA ACAGTGTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TTGCGGACAC GGACAACTTC ATCAACCTCA GCTTCCTGCG GCTCTTCAGG 
GCAGCAAGAC TTATCAAGCT CCTTCGCCAG GGTTACACTA TCCGCATCTT GCTCTGGACG 
TTTGTGCAGT CATTTAAGGC CTTGCCTTAT GTGTGTCTTC TCATTGCAAT GCTCTTCTTC 
ATCTATGCCA TTATTGGCAT GCAGGTATTT GGAAACATTG CACTAAATGA CGAAACCTCC 
ATCAATCGGC ACAATAATTT CCGTACCTTC CTTCAAGCCT TGATGCTTCT GTTCAGGAGT 
GCCACGGGGG AGGCCTGGCA CGAGATCATG CTGTCATGCC TGAGCAACAG GGCCTGCGAC 
CCACTCTCCG GCCTCACCAA GAACGAGTGC GGCAGCGAGT TCGCCTACTT CTACTTCGTG 
TCATTCATCT TCCTCTGCTC CTTCCTGATG TTGAACCTAT TTGTGGCTGT AATCATGGAC 
AACTTTGAAT ACTTGACAAG AGACTCCTCC ATCCTTGGGC CCCATCATTT GGATGAGTTT 
GTCCGTGTCT GGGCTGAATA CGACCCAGCT GCCTGTTGCC GGATTCATTA TAAGGATATG 
TACAATTTGT TACGTGTAAT TGCTCCACCC CTGGGCTTAG GAAAGAAGTG CCCTCATAGG 
GTGGCATACA AGCGCCTGGT GCGGATGAAC ATGCCCATCT CACCCGAGGA TCTGACAGTC 
CACTTCACCT CCACGCTGAT GGCCCTGATC CGCACTGCTC TGGAAATCAA GCTCGCCTCA 
GGGGGTGTTA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AGGAGATATC TCTAGTTTGG 
CCCAACCTGT CCCAGAAGAC ACTGGATTTG CTGGTGCCTC CTCACAAACC TGATGAGATG 
ACTGTGGGGA AGGTCTACGC AGCGCTCATG ATATTTGACT TCTACAAACA GAACAAGAAC 
AGCAGGGAAC AAGTCCACCA GCCTCCTGGG GGCCTGTGCC AGCCCGGACC AGTGTCACTC 
TTCCACCCCT TGAAGGCCAC TTTGGAGCAA ACCCAGCCCT CGGCATTCAG CAGTGCGAAG 
GCATTCCTGC GCCAGAAAAG CTCGGCCTCA CTCAACAATG GAGGCACATT GCCAGCCCCA 
GAGGGTGGGA TCAAAGAGTC CAGTTCGTGG GGGACCCAAC GGACCCAGGA CGTGTTTTAT 
GAAACCAGGA CACCAGCTTT TGAACGAGGC CACTCAGAGG AAATCCCCAT TGAACGGGTG 
GTGGAAATGA GGGAAATCAG CCCCACACTT GCCAATGGAG AGCACCAACC AGGGCTGGAG 
AGCCAGGGTC GGGCGGCTTC CATGCCGCGC CTGGCTGCTG AAACGCAGAG GAGCAAAGCA 
CGGTCACCTG GGAGTTACCT AGCACCCATC CCGGACACAA GCCCCATGAA GCGCTCCATC 
TCCACGCTGA CCCCGCAGCG TCCTCACCCC ATGCACCTCT ATGAGTACTC CCTGGAGCGC 
GTGCCCACGG ACCAGGTGCA TCACCACCAC CACCACCGCT GCCACCGGCG GAAAGAGAAG 
AAGCAGAAGT CCTTGGACAG GACCACACAT CAGCTGGCCG ATGGGGAAGC TGTGGCCCAG 
TCTGGTGAAT CTTCTTCCAA GGACAAGAAG CAAGAGCGTG GACGGTCCCA AGAGAGGAAG 
CAGCACTCTT CCTCCTCCTC AGAAAAGCAG CGCTTCTACT CCTGTGATCG CTATGGCAGC 
CGGGACCGAT CCCAGCCCAA GTCTGCTGAT CAGAGCAGGC CTACTTCACC CAATGGTGGG 
CCTGAGCAAG GTCCCCACAG ACAGTGGTTC GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279278.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134764.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X19, mRNA.

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CloneID	OGa43304
Clone ID Related Accession (Same CDS sequence)	XM_015279278.1
Accession Version	XM_015279278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ACATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGAGTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGTTGCCGG 
ATTCATTATA AGGATATGTA CAATTTGTTA CGTGTAATTG CTCCACCCCT GGGCTTAGGA 
AAGAAGTGCC CTCATAGGGT GGCATACAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGAGGA GCAAAGCACG GTCACCTGGG AGTTACCTAG CACCCATCCC GGACACAAGC 
CCCATGAAGC GCTCCATCTC CACGCTGACC CCGCAGCGTC CTCACCCCAT GCACCTCTAT 
GAGTACTCCC TGGAGCGCGT GCCCACGGAC CAGGTGCATC ACCACCACCA CCACCGCTGC 
CACCGGCGGA AAGAGAAGAA GCAGAAGTCC TTGGACAGGA CCACACATCA GCTGGCCGAT 
GGGGAAGCTG TGGCCCAGTC TGGTGAATCT TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA 
CGGTCCCAAG AGAGGAAGCA GCACTCTTCC TCCTCCTCAG AAAAGCAGCG CTTCTACTCC 
TGTGATCGCT ATGGCAGCCG GGACCGATCC CAGCCCAAGT CTGCTGATCA GAGCAGGCCT 
ACTTCACCCA ATGGTGGGCC TGAGCAAGGT CCCCACAGAC AGTGGTTCGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134764.1
CDS	428..6901
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279278.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279278.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ACATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGAGTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGTTGCCGG 
ATTCATTATA AGGATATGTA CAATTTGTTA CGTGTAATTG CTCCACCCCT GGGCTTAGGA 
AAGAAGTGCC CTCATAGGGT GGCATACAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGAGGA GCAAAGCACG GTCACCTGGG AGTTACCTAG CACCCATCCC GGACACAAGC 
CCCATGAAGC GCTCCATCTC CACGCTGACC CCGCAGCGTC CTCACCCCAT GCACCTCTAT 
GAGTACTCCC TGGAGCGCGT GCCCACGGAC CAGGTGCATC ACCACCACCA CCACCGCTGC 
CACCGGCGGA AAGAGAAGAA GCAGAAGTCC TTGGACAGGA CCACACATCA GCTGGCCGAT 
GGGGAAGCTG TGGCCCAGTC TGGTGAATCT TCTTCCAAGG ACAAGAAGCA AGAGCGTGGA 
CGGTCCCAAG AGAGGAAGCA GCACTCTTCC TCCTCCTCAG AAAAGCAGCG CTTCTACTCC 
TGTGATCGCT ATGGCAGCCG GGACCGATCC CAGCCCAAGT CTGCTGATCA GAGCAGGCCT 
ACTTCACCCA ATGGTGGGCC TGAGCAAGGT CCCCACAGAC AGTGGTTCGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279279.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134765.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X20, mRNA.

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CloneID	OGa43305
Clone ID Related Accession (Same CDS sequence)	XM_015279279.1
Accession Version	XM_015279279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCCAA GCAGCAGAAC 
TCCTCCAAAT CCAAGTCGGT TTGGGAGCAA CGAACCAGCC AGATCCGGAT GCACAACTTC 
CGAGCCAGCT GCGAGGCTCT GTACAACGAA CTGGACCCCG AGGAGCGGGT GCGCTACGCC 
ACGACCCTAC ACATCCGTCC AGACATGAAG ACCCACTTGG ATCGGCCACT GGTGGTAGAG 
CCAAGGGGTG AAGGGAGGAA CAACATCAGT AAGCTGAGCC CTGTGGATGT GCAGGAGGTG 
GAGCAGACCA AAGTCAGCTC TACCGACGGA GCAGAAGCTC CGCGGAAGCA TCACCGGCAT 
CGGGATAAGG AGAAACTGGG AGAGCAAGAG AAGGGTGATG TGACCAAAGA TGAGAATGGG 
GAATCTGGCA TCAACAATAA GGAGGAGAGG CACAGGCAGC ACAGAAGCCG TAGCAAGGAG 
GTGGAAGGGG GCAGCAAGGA AGGGAAAAGT GACCGCAGCA GGGGCCAGGA AGGAGGGAAG 
AGGCACCATC GCCGGGGGTC GGTGGAAGAA GGCGTTGAGA AGGAGCACCG GAGGCACCGC 
ACGCACCGGC ACTCTGCAGA ACGGCAAGGC AAGGAGGGCA ATGGGACCAT CAACGGGGCC 
AGGAGTGAGC GGCGGACCCG CCACCGTGGA GGGTCGAGGT CTGGGAACCG GGAAGGAGAG 
CCTGGGTCCA AGGGCGAGAA TGGAGAGGAG CCTCACAGAC GGCACCGGTT CAGGAGCAGG 
GCGCTGTCGA CGTACGATTC GGTGGAGAAG GAGAACAGGG AGAAGGAAGG GGAGACTGCT 
GAGAAGGAGC ACCAGAATCA TCAGCCCAAG GAGAATCAGT GTGAGATTGA GGCCAGTGGG 
AGTGTGAGCA TCCCGGTGCA CACCCTTCCC AGCACCTATC TGCAGAAAGT GCCAGAGCAG 
CCTGAAGATG CTGACAACCA GAAGAACGTG ACACGCATGA TTCAGCCGCC TCTGGACAAA 
ACCACCACTG TGAACATCCC CGTCACCATC ACCGCCCCGC CAGGAGAAAC CACCGTCATA 
CCTATGAACA ACGTTGAGTT CGAAAGTAAA ACAGAAGAGA AGAAGGACGT TGATGACTTG 
ACGAAAAATG GGCCTAAACC AATCTTGCCT TACAGTTCCA TGTTCATCCT AAGTCCTACG 
AACCCGATCC GGCGTCTGTT CCACTACATC GTCAACCTGC GCTACTTCGA GATGGTCATC 
CTCATCGTCA TCGCTCTCAG CAGCATTGCC CTGGCAGCAG AAGACCCCGT CCAAGCAGAA 
TCACCAAGGA ACGATGCTCT GAAGTACTTG GATTATATAT TCACGGGTGT CTTTACCTTC 
GAGATGGTGA TCAAGATGAT TGACCTGGGG CTGTTACTTC ACCCTGGCTC CTATTTCCGT 
GACCTGTGGA ACATCTTGGA CTTCATTGTG GTCAGTGGGG CCTTGGTGGC ATTTGCTTTC 
TCAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTACA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACACGGACA ACTTCATCAA CCTCAGCTTC CTGCGGCTCT TCAGGGCAGC AAGACTTATC 
AAGCTCCTTC GCCAGGGTTA CACTATCCGC ATCTTGCTCT GGACGTTTGT GCAGTCATTT 
AAGGCCTTGC CTTATGTGTG TCTTCTCATT GCAATGCTCT TCTTCATCTA TGCCATTATT 
GGCATGCAGG TATTTGGAAA CATTGCACTA AATGACGAAA CCTCCATCAA TCGGCACAAT 
AATTTCCGTA CCTTCCTTCA AGCCTTGATG CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC ATGCCTGAGC AACAGGGCCT GCGACCCACT CTCCGGCCTC 
ACCAAGAACG AGTGCGGCAG CGAGTTCGCC TACTTCTACT TCGTGTCATT CATCTTCCTC 
TGCTCCTTCC TGATGTTGAA CCTATTTGTG GCTGTAATCA TGGACAACTT TGAATACTTG 
ACAAGAGACT CCTCCATCCT TGGGCCCCAT CATTTGGATG AGTTTGTCCG TGTCTGGGCT 
GAATACGACC CAGCTGCCTG TTGCCGGATT CATTATAAGG ATATGTACAA TTTGTTACGT 
GTAATTGCTC CACCCCTGGG CTTAGGAAAG AAGTGCCCTC ATAGGGTGGC ATACAAGCGC 
CTGGTGCGGA TGAACATGCC CATCTCACCC GAGGATCTGA CAGTCCACTT CACCTCCACG 
CTGATGGCCC TGATCCGCAC TGCTCTGGAA ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAGGAG ATATCTCTAG TTTGGCCCAA CCTGTCCCAG 
AAGACACTGG ATTTGCTGGT GCCTCCTCAC AAACCTGATG AGATGACTGT GGGGAAGGTC 
TACGCAGCGC TCATGATATT TGACTTCTAC AAACAGAACA AGAACAGCAG GGAACAAGTC 
CACCAGCCTC CTGGGGGCCT GTGCCAGCCC GGACCAGTGT CACTCTTCCA CCCCTTGAAG 
GCCACTTTGG AGCAAACCCA GCCCTCGGCA TTCAGCAGTG CGAAGGCATT CCTGCGCCAG 
AAAAGCTCGG CCTCACTCAA CAATGGAGGC ACATTGCCAG CCCCAGAGGG TGGGATCAAA 
GAGTCCAGTT CGTGGGGGAC CCAACGGACC CAGGACGTGT TTTATGAAAC CAGGACACCA 
GCTTTTGAAC GAGGCCACTC AGAGGAAATC CCCATTGAAC GGGTGGTGGA AATGAGGGAA 
ATCAGCCCCA CACTTGCCAA TGGAGAGCAC CAACCAGGGC TGGAGAGCCA GGGTCGGGCG 
GCTTCCATGC CGCGCCTGGC TGCTGAAACG CAGAGGAGCA AAGCACGGTC ACCTGGGAGT 
TACCTAGCAC CCATCCCGGA CACAAGCCCC ATGAAGCGCT CCATCTCCAC GCTGACCCCG 
CAGCGTCCTC ACCCCATGCA CCTCTATGAG TACTCCCTGG AGCGCGTGCC CACGGACCAG 
GTGCATCACC ACCACCACCA CCGCTGCCAC CGGCGGAAAG AGAAGAAGCA GAAGTCCTTG 
GACAGGACCA CACATCAGCT GGCCGATGGG GAAGCTGTGG CCCAGTCTGG TGAATCTTCT 
TCCAAGGACA AGAAGCAAGA GCGTGGACGG TCCCAAGAGA GGAAGCAGCA CTCTTCCTCC 
TCCTCAGAAA AGCAGCGCTT CTACTCCTGT GATCGCTATG GCAGCCGGGA CCGATCCCAG 
CCCAAGTCTG CTGATCAGAG CAGGCCTACT TCACCCAATG GTGGGCCTGA GCAAGGTCCC 
CACAGACAGT GGTTCGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134765.1
CDS	429..6869
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQWFG

Target ORF information:

RefSeq Version	XM_015279279.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279279.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGGTCTCC TTTTGCCCGT GCCAGTTTGA AAAGTGGGAA GAATGAGAGC 
TCATCCTACT TCAGGAGGAA AGAGAAAATG TTCCGCTTCT TCATCCGGCG CATGGTGAAA 
GCCCAGAGCT TCTACTGGAT TGTGCTCTGC GTGGTTGCCC TCAACACGCT CTGCGTCGCG 
ATGGTGCACT ATGATCAGCC AGAGAAACTC ACCACCGCGC TGTATTTCGC CGAGTTTGTT 
TTTCTGGGTC TGTTCCTTAC TGAGATGTCT TTGAAGATGT ATGGACTGGG GCCAAGAAAT 
TACTTTCATT CCTCATTCAA CTGCTTTGAC TTTGGGGTCA TTGTGGGGAG CATCTTTGAA 
GTCATCTGGG CTGCAGTGAA ACCTGGTACC TCGTTTGGCA TCAGCGTGCT GAGAGCTCTG 
CGGCTGCTGA GGATTTTCAA AGTCACCAAA TACTGGAACT CCCTGAGGAA CCTCGTGGTC 
TCCTTGCTGA ACTCCATGAA GTCCATCATC AGTTTGCTCT TTCTGCTGTT CCTCTTCATT 
GTGGTGTTTG CTCTGCTGGG GATGCAGCTC TTTGGAGGGC AGTTCAATTT TAGAGATGAA 
ACACCAACCA CGAACTTCGA TACTTTTCCA GCTGCCATCC TCACGGTGTT CCAGATCCTG 
ACTGGGGAGG ACTGGAACGC GGTGATGTAC CATGGCATAG AGTCGCAGGG CGGTGTCCAC 
TCGGGGATGT TCTCCTCCAT TTACTTCATC GTCCTGACGT TGTTTGGTAA CTATACACTC 
CTGAACGTCT TCTTGGCCAT CGCCGTTGAC AATCTGGCCA ATGCCCAAGA GCTGACCAAG 
GACGAGGAGG AGATGGAGGA AGCCACCAAT CAGAAGCTCG CCCTGCAGAA GGCCAAGGAG 
GTAGCAGAAG TCAGCCCCAT GTCTGCAGCA AACATTTCCA TAGCCGCCAA GCAGCAGAAC 
TCCTCCAAAT CCAAGTCGGT TTGGGAGCAA CGAACCAGCC AGATCCGGAT GCACAACTTC 
CGAGCCAGCT GCGAGGCTCT GTACAACGAA CTGGACCCCG AGGAGCGGGT GCGCTACGCC 
ACGACCCTAC ACATCCGTCC AGACATGAAG ACCCACTTGG ATCGGCCACT GGTGGTAGAG 
CCAAGGGGTG AAGGGAGGAA CAACATCAGT AAGCTGAGCC CTGTGGATGT GCAGGAGGTG 
GAGCAGACCA AAGTCAGCTC TACCGACGGA GCAGAAGCTC CGCGGAAGCA TCACCGGCAT 
CGGGATAAGG AGAAACTGGG AGAGCAAGAG AAGGGTGATG TGACCAAAGA TGAGAATGGG 
GAATCTGGCA TCAACAATAA GGAGGAGAGG CACAGGCAGC ACAGAAGCCG TAGCAAGGAG 
GTGGAAGGGG GCAGCAAGGA AGGGAAAAGT GACCGCAGCA GGGGCCAGGA AGGAGGGAAG 
AGGCACCATC GCCGGGGGTC GGTGGAAGAA GGCGTTGAGA AGGAGCACCG GAGGCACCGC 
ACGCACCGGC ACTCTGCAGA ACGGCAAGGC AAGGAGGGCA ATGGGACCAT CAACGGGGCC 
AGGAGTGAGC GGCGGACCCG CCACCGTGGA GGGTCGAGGT CTGGGAACCG GGAAGGAGAG 
CCTGGGTCCA AGGGCGAGAA TGGAGAGGAG CCTCACAGAC GGCACCGGTT CAGGAGCAGG 
GCGCTGTCGA CGTACGATTC GGTGGAGAAG GAGAACAGGG AGAAGGAAGG GGAGACTGCT 
GAGAAGGAGC ACCAGAATCA TCAGCCCAAG GAGAATCAGT GTGAGATTGA GGCCAGTGGG 
AGTGTGAGCA TCCCGGTGCA CACCCTTCCC AGCACCTATC TGCAGAAAGT GCCAGAGCAG 
CCTGAAGATG CTGACAACCA GAAGAACGTG ACACGCATGA TTCAGCCGCC TCTGGACAAA 
ACCACCACTG TGAACATCCC CGTCACCATC ACCGCCCCGC CAGGAGAAAC CACCGTCATA 
CCTATGAACA ACGTTGAGTT CGAAAGTAAA ACAGAAGAGA AGAAGGACGT TGATGACTTG 
ACGAAAAATG GGCCTAAACC AATCTTGCCT TACAGTTCCA TGTTCATCCT AAGTCCTACG 
AACCCGATCC GGCGTCTGTT CCACTACATC GTCAACCTGC GCTACTTCGA GATGGTCATC 
CTCATCGTCA TCGCTCTCAG CAGCATTGCC CTGGCAGCAG AAGACCCCGT CCAAGCAGAA 
TCACCAAGGA ACGATGCTCT GAAGTACTTG GATTATATAT TCACGGGTGT CTTTACCTTC 
GAGATGGTGA TCAAGATGAT TGACCTGGGG CTGTTACTTC ACCCTGGCTC CTATTTCCGT 
GACCTGTGGA ACATCTTGGA CTTCATTGTG GTCAGTGGGG CCTTGGTGGC ATTTGCTTTC 
TCAGGAACCA AAGGCAAGGA CATCAACACG ATCAAGTCGC TGCGGGTTCT GCGGGTCCTA 
CGACCACTGA AGACCATTAA ACGGCTGCCA AAACTAAAGG CTGTCTTTGA CTGCGTGGTG 
AATTCCCTGA AGAACGTCCT CAACATCCTC ATTGTCTACA TGCTCTTCAT GTTCATCTTT 
GCTGTCATTG CTGTGCAGCT CTTCAAAGGC AGATTCTTCT ACTGCACTGA TGAGTCGAAA 
GAGCTGGAGA AGGATTGCAG GGGACAATAC CTTGACTATG AAAAAAATGA GGTGGAGGCG 
CAACCCAGGG AATGGAAAAA ATATGAGTTC CATTACGACA ATGTGCTGTG GGCTCTGCTG 
ACACTTTTCA CTGTCTCCAC AGGAGAAGGG TGGCCAACTG TGCTGAAGCA CTCAGTGGAT 
GCTACCTACG AGGAACAGGG TCCCAGCCCT GGCTACCGAA TGGAAATGTC TATCTTTTAC 
GTGGTGTATT TTGTTGTCTT CCCTTTTTTC TTTGTGAACA TCTTTGTGGC GTTAATCATA 
ATCACCTTCC AAGAGCAAGG AGATAAAGTG ATGTCAGAAT GCAGCCTAGA GAAAAATGAG 
AGGGCCTGCA TTGACTTCGC CATAAGTGCC AAGCCACTGA CCCGCTACAT GCCTCAGAAC 
AAGCAGTCAT TTCAGTACAA GATGTGGAAA TTTGTGGTGT CCCCGCCCTT TGAGTATTTT 
ATCATGGTCA TGATTGCACT CAATACTATT GTTCTCATGA TGAAGTTCTA TGATGCTCCA 
GAAGCATATG AAGAAATGTT GAAATGCCTG AATATTGTCT TTACATCCAT GTTTTCAATG 
GAGTGTGTGC TGAAGATCAT TGCCTTTGGT GTGCTGAATT ATTTCCGAGA TGCCTGGAAT 
GTCTTTGATT TTGTAACAGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GACACGGACA ACTTCATCAA CCTCAGCTTC CTGCGGCTCT TCAGGGCAGC AAGACTTATC 
AAGCTCCTTC GCCAGGGTTA CACTATCCGC ATCTTGCTCT GGACGTTTGT GCAGTCATTT 
AAGGCCTTGC CTTATGTGTG TCTTCTCATT GCAATGCTCT TCTTCATCTA TGCCATTATT 
GGCATGCAGG TATTTGGAAA CATTGCACTA AATGACGAAA CCTCCATCAA TCGGCACAAT 
AATTTCCGTA CCTTCCTTCA AGCCTTGATG CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC ATGCCTGAGC AACAGGGCCT GCGACCCACT CTCCGGCCTC 
ACCAAGAACG AGTGCGGCAG CGAGTTCGCC TACTTCTACT TCGTGTCATT CATCTTCCTC 
TGCTCCTTCC TGATGTTGAA CCTATTTGTG GCTGTAATCA TGGACAACTT TGAATACTTG 
ACAAGAGACT CCTCCATCCT TGGGCCCCAT CATTTGGATG AGTTTGTCCG TGTCTGGGCT 
GAATACGACC CAGCTGCCTG TTGCCGGATT CATTATAAGG ATATGTACAA TTTGTTACGT 
GTAATTGCTC CACCCCTGGG CTTAGGAAAG AAGTGCCCTC ATAGGGTGGC ATACAAGCGC 
CTGGTGCGGA TGAACATGCC CATCTCACCC GAGGATCTGA CAGTCCACTT CACCTCCACG 
CTGATGGCCC TGATCCGCAC TGCTCTGGAA ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAGGAG ATATCTCTAG TTTGGCCCAA CCTGTCCCAG 
AAGACACTGG ATTTGCTGGT GCCTCCTCAC AAACCTGATG AGATGACTGT GGGGAAGGTC 
TACGCAGCGC TCATGATATT TGACTTCTAC AAACAGAACA AGAACAGCAG GGAACAAGTC 
CACCAGCCTC CTGGGGGCCT GTGCCAGCCC GGACCAGTGT CACTCTTCCA CCCCTTGAAG 
GCCACTTTGG AGCAAACCCA GCCCTCGGCA TTCAGCAGTG CGAAGGCATT CCTGCGCCAG 
AAAAGCTCGG CCTCACTCAA CAATGGAGGC ACATTGCCAG CCCCAGAGGG TGGGATCAAA 
GAGTCCAGTT CGTGGGGGAC CCAACGGACC CAGGACGTGT TTTATGAAAC CAGGACACCA 
GCTTTTGAAC GAGGCCACTC AGAGGAAATC CCCATTGAAC GGGTGGTGGA AATGAGGGAA 
ATCAGCCCCA CACTTGCCAA TGGAGAGCAC CAACCAGGGC TGGAGAGCCA GGGTCGGGCG 
GCTTCCATGC CGCGCCTGGC TGCTGAAACG CAGAGGAGCA AAGCACGGTC ACCTGGGAGT 
TACCTAGCAC CCATCCCGGA CACAAGCCCC ATGAAGCGCT CCATCTCCAC GCTGACCCCG 
CAGCGTCCTC ACCCCATGCA CCTCTATGAG TACTCCCTGG AGCGCGTGCC CACGGACCAG 
GTGCATCACC ACCACCACCA CCGCTGCCAC CGGCGGAAAG AGAAGAAGCA GAAGTCCTTG 
GACAGGACCA CACATCAGCT GGCCGATGGG GAAGCTGTGG CCCAGTCTGG TGAATCTTCT 
TCCAAGGACA AGAAGCAAGA GCGTGGACGG TCCCAAGAGA GGAAGCAGCA CTCTTCCTCC 
TCCTCAGAAA AGCAGCGCTT CTACTCCTGT GATCGCTATG GCAGCCGGGA CCGATCCCAG 
CCCAAGTCTG CTGATCAGAG CAGGCCTACT TCACCCAATG GTGGGCCTGA GCAAGGTCCC 
CACAGACAGT GGTTCGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279280.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134766.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X21, mRNA.

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CloneID	OGa43306
Clone ID Related Accession (Same CDS sequence)	XM_015279280.1
Accession Version	XM_015279280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ACATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGAGTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGCGGGCGC 
ATCAGTTACA CAGACATGTA TGAGATGCTG AGACACATGT CCCCACCTCT AGGCCTTGGA 
AAGAAATGCC CTGCTCGCGT TGCCTATAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGCCCA TCCCGGACAC AAGCCCCATG AAGCGCTCCA TCTCCACGCT GACCCCGCAG 
CGTCCTCACC CCATGCACCT CTATGAGTAC TCCCTGGAGC GCGTGCCCAC GGACCAGGTG 
CATCACCACC ACCACCACCG CTGCCACCGG CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC 
AGGACCACAC ATCAGCTGGC CGATGGGGAA GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC 
AAGGACAAGA AGCAAGAGCG TGGACGGTCC CAAGAGAGGA AGCAGCACTC TTCCTCCTCC 
TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT CGCTATGGCA GCCGGGACCG ATCCCAGCCC 
AAGTCTGCTG ATCAGAGCAG GCCTACTTCA CCCAATGGTG GGCCTGAGCA AGGTCCCCAC 
AGACAGGGCA GTGGTTCGGT TAACGGGAGC CCCTTGCTGT CGACATCTGG TGCTAGTACC 
CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG ACCCCCCTGA CCCCACGCCC CAGCATCACT 
TACAAGACCG CCAACTCCTC GCCCGTACAC TTCAGCACGT CGCCGGGCGG CCTCCCGCCC 
TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG TCTGAGCACA ATGCACTGCT GCGTGGGGAC 
CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC ATCGGCTCCG ACCCTTACCT GGGCCACCGC 
GACGCCGCCG ACAGCCCCAT AGGCGCAGCG CCCGAGGACA CGCTCACCTT CGAGGAAGCC 
GTGGCCACCA ACTCGGGGCG TTCCTCCAGG ACTTCTTACG TCTCCTCCTT GACCTCCCAG 
TCTCACCAAG CACGCCGGGT GCCCAATGGC TACCATTACA CGCTGGGGCT CAACACGGGG 
CCCGGCACTG GCACCAGGGG ACGGAGCTAC TACCACGAAG CCGACGAGGA TGACTGGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134766.1
CDS	424..7386
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279280.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279280.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC CAAGCAGCAG AACTCCTCCA AATCCAAGTC GGTTTGGGAG 
CAACGAACCA GCCAGATCCG GATGCACAAC TTCCGAGCCA GCTGCGAGGC TCTGTACAAC 
GAACTGGACC CCGAGGAGCG GGTGCGCTAC GCCACGACCC TACACATCCG TCCAGACATG 
AAGACCCACT TGGATCGGCC ACTGGTGGTA GAGCCAAGGG GTGAAGGGAG GAACAACATC 
AGTAAGCTGA GCCCTGTGGA TGTGCAGGAG GTGGAGCAGA CCAAAGTCAG CTCTACCGAC 
GGAGCAGAAG CTCCGCGGAA GCATCACCGG CATCGGGATA AGGAGAAACT GGGAGAGCAA 
GAGAAGGGTG ATGTGACCAA AGATGAGAAT GGGGAATCTG GCATCAACAA TAAGGAGGAG 
AGGCACAGGC AGCACAGAAG CCGTAGCAAG GAGGTGGAAG GGGGCAGCAA GGAAGGGAAA 
AGTGACCGCA GCAGGGGCCA GGAAGGAGGG AAGAGGCACC ATCGCCGGGG GTCGGTGGAA 
GAAGGCGTTG AGAAGGAGCA CCGGAGGCAC CGCACGCACC GGCACTCTGC AGAACGGCAA 
GGCAAGGAGG GCAATGGGAC CATCAACGGG GCCAGGAGTG AGCGGCGGAC CCGCCACCGT 
GGAGGGTCGA GGTCTGGGAA CCGGGAAGGA GAGCCTGGGT CCAAGGGCGA GAATGGAGAG 
GAGCCTCACA GACGGCACCG GTTCAGGAGC AGGGCGCTGT CGACGTACGA TTCGGTGGAG 
AAGGAGAACA GGGAGAAGGA AGGGGAGACT GCTGAGAAGG AGCACCAGAA TCATCAGCCC 
AAGGAGAATC AGTGTGAGAT TGAGGCCAGT GGGAGTGTGA GCATCCCGGT GCACACCCTT 
CCCAGCACCT ATCTGCAGAA AGTGCCAGAG CAGCCTGAAG ATGCTGACAA CCAGAAGAAC 
GTGACACGCA TGATTCAGCC GCCTCTGGAC AAAACCACCA CTGTGAACAT CCCCGTCACC 
ATCACCGCCC CGCCAGGAGA AACCACCGTC ATACCTATGA ACAACGTTGA GTTCGAAAGT 
AAAACAGAAG AGAAGAAGGA CGTTGATGAC TTGACGAAAA ATGGGCCTAA ACCAATCTTG 
CCTTACAGTT CCATGTTCAT CCTAAGTCCT ACGAACCCGA TCCGGCGTCT GTTCCACTAC 
ATCGTCAACC TGCGCTACTT CGAGATGGTC ATCCTCATCG TCATCGCTCT CAGCAGCATT 
GCCCTGGCAG CAGAAGACCC CGTCCAAGCA GAATCACCAA GGAACGATGC TCTGAAGTAC 
TTGGATTATA TATTCACGGG TGTCTTTACC TTCGAGATGG TGATCAAGAT GATTGACCTG 
GGGCTGTTAC TTCACCCTGG CTCCTATTTC CGTGACCTGT GGAACATCTT GGACTTCATT 
GTGGTCAGTG GGGCCTTGGT GGCATTTGCT TTCTCAGGAA CCAAAGGCAA GGACATCAAC 
ACGATCAAGT CGCTGCGGGT TCTGCGGGTC CTACGACCAC TGAAGACCAT TAAACGGCTG 
CCAAAACTAA AGGCTGTCTT TGACTGCGTG GTGAATTCCC TGAAGAACGT CCTCAACATC 
CTCATTGTCT ACATGCTCTT CATGTTCATC TTTGCTGTCA TTGCTGTGCA GCTCTTCAAA 
GGCAGATTCT TCTACTGCAC TGATGAGTCG AAAGAGCTGG AGAAGGATTG CAGGGGACAA 
TACCTTGACT ATGAAAAAAA TGAGGTGGAG GCGCAACCCA GGGAATGGAA AAAATATGAG 
TTCCATTACG ACAATGTGCT GTGGGCTCTG CTGACACTTT TCACTGTCTC CACAGGAGAA 
GGGTGGCCAA CTGTGCTGAA GCACTCAGTG GATGCTACCT ACGAGGAACA GGGTCCCAGC 
CCTGGCTACC GAATGGAAAT GTCTATCTTT TACGTGGTGT ATTTTGTTGT CTTCCCTTTT 
TTCTTTGTGA ACATCTTTGT GGCGTTAATC ATAATCACCT TCCAAGAGCA AGGAGATAAA 
GTGATGTCAG AATGCAGCCT AGAGAAAAAT GAGAGGGCCT GCATTGACTT CGCCATAAGT 
GCCAAGCCAC TGACCCGCTA CATGCCTCAG AACAAGCAGT CATTTCAGTA CAAGATGTGG 
AAATTTGTGG TGTCCCCGCC CTTTGAGTAT TTTATCATGG TCATGATTGC ACTCAATACT 
ATTGTTCTCA TGATGAAGTT CTATGATGCT CCAGAAGCAT ATGAAGAAAT GTTGAAATGC 
CTGAATATTG TCTTTACATC CATGTTTTCA ATGGAGTGTG TGCTGAAGAT CATTGCCTTT 
GGTGTGCTGA ATTATTTCCG AGATGCCTGG AATGTCTTTG ATTTTGTAAC AGTGTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATT GCGGACACGG ACAACTTCAT CAACCTCAGC 
TTCCTGCGGC TCTTCAGGGC AGCAAGACTT ATCAAGCTCC TTCGCCAGGG TTACACTATC 
CGCATCTTGC TCTGGACGTT TGTGCAGTCA TTTAAGGCCT TGCCTTATGT GTGTCTTCTC 
ATTGCAATGC TCTTCTTCAT CTATGCCATT ATTGGCATGC AGGTATTTGG AAACATTGCA 
CTAAATGACG AAACCTCCAT CAATCGGCAC AATAATTTCC GTACCTTCCT TCAAGCCTTG 
ATGCTTCTGT TCAGGAGTGC CACGGGGGAG GCCTGGCACG AGATCATGCT GTCATGCCTG 
AGCAACAGGG CCTGCGACCC ACTCTCCGGC CTCACCAAGA ACGAGTGCGG CAGCGAGTTC 
GCCTACTTCT ACTTCGTGTC ATTCATCTTC CTCTGCTCCT TCCTGATGTT GAACCTATTT 
GTGGCTGTAA TCATGGACAA CTTTGAATAC TTGACAAGAG ACTCCTCCAT CCTTGGGCCC 
CATCATTTGG ATGAGTTTGT CCGTGTCTGG GCTGAATACG ACCCAGCTGC CTGCGGGCGC 
ATCAGTTACA CAGACATGTA TGAGATGCTG AGACACATGT CCCCACCTCT AGGCCTTGGA 
AAGAAATGCC CTGCTCGCGT TGCCTATAAG CGCCTGGTGC GGATGAACAT GCCCATCTCA 
CCCGAGGATC TGACAGTCCA CTTCACCTCC ACGCTGATGG CCCTGATCCG CACTGCTCTG 
GAAATCAAGC TCGCCTCAGG GGGTGTTAAG CAGCACCAGT GTGATGCTGA GCTGAGAAAG 
GAGATATCTC TAGTTTGGCC CAACCTGTCC CAGAAGACAC TGGATTTGCT GGTGCCTCCT 
CACAAACCTG ATGAGATGAC TGTGGGGAAG GTCTACGCAG CGCTCATGAT ATTTGACTTC 
TACAAACAGA ACAAGAACAG CAGGGAACAA GTCCACCAGC CTCCTGGGGG CCTGTGCCAG 
CCCGGACCAG TGTCACTCTT CCACCCCTTG AAGGCCACTT TGGAGCAAAC CCAGCCCTCG 
GCATTCAGCA GTGCGAAGGC ATTCCTGCGC CAGAAAAGCT CGGCCTCACT CAACAATGGA 
GGCACATTGC CAGCCCCAGA GGGTGGGATC AAAGAGTCCA GTTCGTGGGG GACCCAACGG 
ACCCAGGACG TGTTTTATGA AACCAGGACA CCAGCTTTTG AACGAGGCCA CTCAGAGGAA 
ATCCCCATTG AACGGGTGGT GGAAATGAGG GAAATCAGCC CCACACTTGC CAATGGAGAG 
CACCAACCAG GGCTGGAGAG CCAGGGTCGG GCGGCTTCCA TGCCGCGCCT GGCTGCTGAA 
ACGCAGCCCA TCCCGGACAC AAGCCCCATG AAGCGCTCCA TCTCCACGCT GACCCCGCAG 
CGTCCTCACC CCATGCACCT CTATGAGTAC TCCCTGGAGC GCGTGCCCAC GGACCAGGTG 
CATCACCACC ACCACCACCG CTGCCACCGG CGGAAAGAGA AGAAGCAGAA GTCCTTGGAC 
AGGACCACAC ATCAGCTGGC CGATGGGGAA GCTGTGGCCC AGTCTGGTGA ATCTTCTTCC 
AAGGACAAGA AGCAAGAGCG TGGACGGTCC CAAGAGAGGA AGCAGCACTC TTCCTCCTCC 
TCAGAAAAGC AGCGCTTCTA CTCCTGTGAT CGCTATGGCA GCCGGGACCG ATCCCAGCCC 
AAGTCTGCTG ATCAGAGCAG GCCTACTTCA CCCAATGGTG GGCCTGAGCA AGGTCCCCAC 
AGACAGGGCA GTGGTTCGGT TAACGGGAGC CCCTTGCTGT CGACATCTGG TGCTAGTACC 
CCCTGCCGGG GTCGGAGGCA GCTCCCGCAG ACCCCCCTGA CCCCACGCCC CAGCATCACT 
TACAAGACCG CCAACTCCTC GCCCGTACAC TTCAGCACGT CGCCGGGCGG CCTCCCGCCC 
TTCTCCCCGG GCCGCCTGAG CCGCGGGCTG TCTGAGCACA ATGCACTGCT GCGTGGGGAC 
CAGCAGCCGC CCCCGGCCGC GGTTGCCCGC ATCGGCTCCG ACCCTTACCT GGGCCACCGC 
GACGCCGCCG ACAGCCCCAT AGGCGCAGCG CCCGAGGACA CGCTCACCTT CGAGGAAGCC 
GTGGCCACCA ACTCGGGGCG TTCCTCCAGG ACTTCTTACG TCTCCTCCTT GACCTCCCAG 
TCTCACCAAG CACGCCGGGT GCCCAATGGC TACCATTACA CGCTGGGGCT CAACACGGGG 
CCCGGCACTG GCACCAGGGG ACGGAGCTAC TACCACGAAG CCGACGAGGA TGACTGGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_015279281.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015134767.1 Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X22, mRNA.

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CloneID	OGa43307
Clone ID Related Accession (Same CDS sequence)	XM_015279281.1
Accession Version	XM_015279281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC TTTTGTAAAG CAAACTCGAG GTACTGTATC TCGCAGCTCG 
TCAGTCTCCA GCGTAAATTC ACCCAAGCAG CAGAACTCCT CCAAATCCAA GTCGGTTTGG 
GAGCAACGAA CCAGCCAGAT CCGGATGCAC AACTTCCGAG CCAGCTGCGA GGCTCTGTAC 
AACGAACTGG ACCCCGAGGA GCGGGTGCGC TACGCCACGA CCCTACACAT CCGTCCAGAC 
ATGAAGACCC ACTTGGATCG GCCACTGGTG GTAGAGCCAA GGGGTGAAGG GAGGAACAAC 
ATCAGTAAGC TGAGCCCTGT GGATGTGCAG GAGGTGGAGC AGACCAAAGT CAGCTCTACC 
GACGGAGCAG AAGCTCCGCG GAAGCATCAC CGGCATCGGG ATAAGGAGAA ACTGGGAGAG 
CAAGAGAAGG GTGATGTGAC CAAAGATGAG AATGGGGAAT CTGGCATCAA CAATAAGGAG 
GAGAGGCACA GGCAGCACAG AAGCCGTAGC AAGGAGGTGG AAGGGGGCAG CAAGGAAGGG 
AAAAGTGACC GCAGCAGGGG CCAGGAAGGA GGGAAGAGGC ACCATCGCCG GGGGTCGGTG 
GAAGAAGGCG TTGAGAAGGA GCACCGGAGG CACCGCACGC ACCGGCACTC TGCAGAACGG 
CAAGGCAAGG AGGGCAATGG GACCATCAAC GGGGCCAGGA GTGAGCGGCG GACCCGCCAC 
CGTGGAGGGT CGAGGTCTGG GAACCGGGAA GGAGAGCCTG GGTCCAAGGG CGAGAATGGA 
GAGGAGCCTC ACAGACGGCA CCGGTTCAGG AGCAGGGCGC TGTCGACGTA CGATTCGGTG 
GAGAAGGAGA ACAGGGAGAA GGAAGGGGAG ACTGCTGAGA AGGAGCACCA GAATCATCAG 
CCCAAGGAGA ATCAGTGTGA GATTGAGGCC AGTGGGAGTG TGAGCATCCC GGTGCACACC 
CTTCCCAGCA CCTATCTGCA GAAAGTGCCA GAGCAGCCTG AAGATGCTGA CAACCAGAAG 
AACGTGACAC GCATGATTCA GCCGCCTCTG GACAAAACCA CCACTGTGAA CATCCCCGTC 
ACCATCACCG CCCCGCCAGG AGAAACCACC GTCATACCTA TGAACAACGT TGAGTTCGAA 
AGTAAAACAG AAGAGAAGAA GGACGTTGAT GACTTGACGA AAAATGGGCC TAAACCAATC 
TTGCCTTACA GTTCCATGTT CATCCTAAGT CCTACGAACC CGATCCGGCG TCTGTTCCAC 
TACATCGTCA ACCTGCGCTA CTTCGAGATG GTCATCCTCA TCGTCATCGC TCTCAGCAGC 
ATTGCCCTGG CAGCAGAAGA CCCCGTCCAA GCAGAATCAC CAAGGAACGA TGCTCTGAAG 
TACTTGGATT ATATATTCAC GGGTGTCTTT ACCTTCGAGA TGGTGATCAA GATGATTGAC 
CTGGGGCTGT TACTTCACCC TGGCTCCTAT TTCCGTGACC TGTGGAACAT CTTGGACTTC 
ATTGTGGTCA GTGGGGCCTT GGTGGCATTT GCTTTCTCAG GAACCAAAGG CAAGGACATC 
AACACGATCA AGTCGCTGCG GGTTCTGCGG GTCCTACGAC CACTGAAGAC CATTAAACGG 
CTGCCAAAAC TAAAGGCTGT CTTTGACTGC GTGGTGAATT CCCTGAAGAA CGTCCTCAAC 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCTG TCATTGCTGT GCAGCTCTTC 
AAAGGCAGAT TCTTCTACTG CACTGATGAG TCGAAAGAGC TGGAGAAGGA TTGCAGGGGA 
CAATACCTTG ACTATGAAAA AAATGAGGTG GAGGCGCAAC CCAGGGAATG GAAAAAATAT 
GAGTTCCATT ACGACAATGT GCTGTGGGCT CTGCTGACAC TTTTCACTGT CTCCACAGGA 
GAAGGGTGGC CAACTGTGCT GAAGCACTCA GTGGATGCTA CCTACGAGGA ACAGGGTCCC 
AGCCCTGGCT ACCGAATGGA AATGTCTATC TTTTACGTGG TGTATTTTGT TGTCTTCCCT 
TTTTTCTTTG TGAACATCTT TGTGGCGTTA ATCATAATCA CCTTCCAAGA GCAAGGAGAT 
AAAGTGATGT CAGAATGCAG CCTAGAGAAA AATGAGAGGG CCTGCATTGA CTTCGCCATA 
AGTGCCAAGC CACTGACCCG CTACATGCCT CAGAACAAGC AGTCATTTCA GTACAAGATG 
TGGAAATTTG TGGTGTCCCC GCCCTTTGAG TATTTTATCA TGGTCATGAT TGCACTCAAT 
ACTATTGTTC TCATGATGAA GTTCTATGAT GCTCCAGAAG CATATGAAGA AATGTTGAAA 
TGCCTGAATA TTGTCTTTAC ATCCATGTTT TCAATGGAGT GTGTGCTGAA GATCATTGCC 
TTTGGTGTGC TGAATTATTT CCGAGATGCC TGGAATGTCT TTGATTTTGT AACAGTGTTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGGACA ACTTCATCAA CCTCAGCTTC 
CTGCGGCTCT TCAGGGCAGC AAGACTTATC AAGCTCCTTC GCCAGGGTTA CACTATCCGC 
ATCTTGCTCT GGACGTTTGT GCAGTCATTT AAGGCCTTGC CTTATGTGTG TCTTCTCATT 
GCAATGCTCT TCTTCATCTA TGCCATTATT GGCATGCAGG TATTTGGAAA CATTGCACTA 
AATGACGAAA CCTCCATCAA TCGGCACAAT AATTTCCGTA CCTTCCTTCA AGCCTTGATG 
CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC TGGCACGAGA TCATGCTGTC ATGCCTGAGC 
AACAGGGCCT GCGACCCACT CTCCGGCCTC ACCAAGAACG AGTGCGGCAG CGAGTTCGCC 
TACTTCTACT TCGTGTCATT CATCTTCCTC TGCTCCTTCC TGATGTTGAA CCTATTTGTG 
GCTGTAATCA TGGACAACTT TGAATACTTG ACAAGAGACT CCTCCATCCT TGGGCCCCAT 
CATTTGGATG AGTTTGTCCG TGTCTGGGCT GAATACGACC CAGCTGCCTG CGGGCGCATC 
AGTTACACAG ACATGTATGA GATGCTGAGA CACATGTCCC CACCTCTAGG CCTTGGAAAG 
AAATGCCCTG CTCGCGTTGC CTATAAGCGC CTGGTGCGGA TGAACATGCC CATCTCACCC 
GAGGATCTGA CAGTCCACTT CACCTCCACG CTGATGGCCC TGATCCGCAC TGCTCTGGAA 
ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG CACCAGTGTG ATGCTGAGCT GAGAAAGGAG 
ATATCTCTAG TTTGGCCCAA CCTGTCCCAG AAGACACTGG ATTTGCTGGT GCCTCCTCAC 
AAACCTGATG AGATGACTGT GGGGAAGGTC TACGCAGCGC TCATGATATT TGACTTCTAC 
AAACAGAACA AGAACAGCAG GGAACAAGTC CACCAGCCTC CTGGGGGCCT GTGCCAGCCC 
GGACCAGTGT CACTCTTCCA CCCCTTGAAG GCCACTTTGG AGCAAACCCA GCCCTCGGCA 
TTCAGCAGTG CGAAGGCATT CCTGCGCCAG AAAAGCTCGG CCTCACTCAA CAATGGAGGC 
ACATTGCCAG CCCCAGAGGG TGGGATCAAA GAGTCCAGTT CGTGGGGGAC CCAACGGACC 
CAGGACGTGT TTTATGAAAC CAGGACACCA GCTTTTGAAC GAGGCCACTC AGAGGAAATC 
CCCATTGAAC GGGTGGTGGA AATGAGGGAA ATCAGCCCCA CACTTGCCAA TGGAGAGCAC 
CAACCAGGGC TGGAGAGCCA GGGTCGGGCG GCTTCCATGC CGCGCCTGGC TGCTGAAACG 
CAGCCCATCC CGGACACAAG CCCCATGAAG CGCTCCATCT CCACGCTGAC CCCGCAGCGT 
CCTCACCCCA TGCACCTCTA TGAGTACTCC CTGGAGCGCG TGCCCACGGA CCAGGTGCAT 
CACCACCACC ACCACCGCTG CCACCGGCGG AAAGAGAAGA AGCAGAAGTC CTTGGACAGG 
ACCACACATC AGCTGGCCGA TGGGGAAGCT GTGGCCCAGT CTGGTGAATC TTCTTCCAAG 
GACAAGAAGC AAGAGCGTGG ACGGTCCCAA GAGAGGAAGC AGCACTCTTC CTCCTCCTCA 
GAAAAGCAGC GCTTCTACTC CTGTGATCGC TATGGCAGCC GGGACCGATC CCAGCCCAAG 
TCTGCTGATC AGAGCAGGCC TACTTCACCC AATGGTGGGC CTGAGCAAGG TCCCCACAGA 
CAGGGCAGTG GTTCGGTTAA CGGGAGCCCC TTGCTGTCGA CATCTGGTGC TAGTACCCCC 
TGCCGGGGTC GGAGGCAGCT CCCGCAGACC CCCCTGACCC CACGCCCCAG CATCACTTAC 
AAGACCGCCA ACTCCTCGCC CGTACACTTC AGCACGTCGC CGGGCGGCCT CCCGCCCTTC 
TCCCCGGGCC GCCTGAGCCG CGGGCTGTCT GAGCACAATG CACTGCTGCG TGGGGACCAG 
CAGCCGCCCC CGGCCGCGGT TGCCCGCATC GGCTCCGACC CTTACCTGGG CCACCGCGAC 
GCCGCCGACA GCCCCATAGG CGCAGCGCCC GAGGACACGC TCACCTTCGA GGAAGCCGTG 
GCCACCAACT CGGGGCGTTC CTCCAGGACT TCTTACGTCT CCTCCTTGAC CTCCCAGTCT 
CACCAAGCAC GCCGGGTGCC CAATGGCTAC CATTACACGC TGGGGCTCAA CACGGGGCCC 
GGCACTGGCA CCAGGGGACG GAGCTACTAC CACGAAGCCG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015134767.1
CDS	425..7444
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNETGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACGRISYTDMYEMLRHMSPPLGLGKKCPARVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKNSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	XM_015279281.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, N type, alpha 1B subunit (CACNA1B), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279281.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGACTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTCC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATGAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGTGTT GAAAAGAGCT GCAATAAAGA AGAGCAAAAA CGACCTGATT 
CATGCTGAAG AAGGTGAGGA CCATTTCACA GACATTTGCT CCGTTGGGTC TCCTTTTGCC 
CGTGCCAGTT TGAAAAGTGG GAAGAATGAG AGCTCATCCT ACTTCAGGAG GAAAGAGAAA 
ATGTTCCGCT TCTTCATCCG GCGCATGGTG AAAGCCCAGA GCTTCTACTG GATTGTGCTC 
TGCGTGGTTG CCCTCAACAC GCTCTGCGTC GCGATGGTGC ACTATGATCA GCCAGAGAAA 
CTCACCACCG CGCTGTATTT CGCCGAGTTT GTTTTTCTGG GTCTGTTCCT TACTGAGATG 
TCTTTGAAGA TGTATGGACT GGGGCCAAGA AATTACTTTC ATTCCTCATT CAACTGCTTT 
GACTTTGGGG TCATTGTGGG GAGCATCTTT GAAGTCATCT GGGCTGCAGT GAAACCTGGT 
ACCTCGTTTG GCATCAGCGT GCTGAGAGCT CTGCGGCTGC TGAGGATTTT CAAAGTCACC 
AAATACTGGA ACTCCCTGAG GAACCTCGTG GTCTCCTTGC TGAACTCCAT GAAGTCCATC 
ATCAGTTTGC TCTTTCTGCT GTTCCTCTTC ATTGTGGTGT TTGCTCTGCT GGGGATGCAG 
CTCTTTGGAG GGCAGTTCAA TTTTAGAGAT GAAACACCAA CCACGAACTT CGATACTTTT 
CCAGCTGCCA TCCTCACGGT GTTCCAGATC CTGACTGGGG AGGACTGGAA CGCGGTGATG 
TACCATGGCA TAGAGTCGCA GGGCGGTGTC CACTCGGGGA TGTTCTCCTC CATTTACTTC 
ATCGTCCTGA CGTTGTTTGG TAACTATACA CTCCTGAACG TCTTCTTGGC CATCGCCGTT 
GACAATCTGG CCAATGCCCA AGAGCTGACC AAGGACGAGG AGGAGATGGA GGAAGCCACC 
AATCAGAAGC TCGCCCTGCA GAAGGCCAAG GAGGTAGCAG AAGTCAGCCC CATGTCTGCA 
GCAAACATTT CCATAGCCGC TTTTGTAAAG CAAACTCGAG GTACTGTATC TCGCAGCTCG 
TCAGTCTCCA GCGTAAATTC ACCCAAGCAG CAGAACTCCT CCAAATCCAA GTCGGTTTGG 
GAGCAACGAA CCAGCCAGAT CCGGATGCAC AACTTCCGAG CCAGCTGCGA GGCTCTGTAC 
AACGAACTGG ACCCCGAGGA GCGGGTGCGC TACGCCACGA CCCTACACAT CCGTCCAGAC 
ATGAAGACCC ACTTGGATCG GCCACTGGTG GTAGAGCCAA GGGGTGAAGG GAGGAACAAC 
ATCAGTAAGC TGAGCCCTGT GGATGTGCAG GAGGTGGAGC AGACCAAAGT CAGCTCTACC 
GACGGAGCAG AAGCTCCGCG GAAGCATCAC CGGCATCGGG ATAAGGAGAA ACTGGGAGAG 
CAAGAGAAGG GTGATGTGAC CAAAGATGAG AATGGGGAAT CTGGCATCAA CAATAAGGAG 
GAGAGGCACA GGCAGCACAG AAGCCGTAGC AAGGAGGTGG AAGGGGGCAG CAAGGAAGGG 
AAAAGTGACC GCAGCAGGGG CCAGGAAGGA GGGAAGAGGC ACCATCGCCG GGGGTCGGTG 
GAAGAAGGCG TTGAGAAGGA GCACCGGAGG CACCGCACGC ACCGGCACTC TGCAGAACGG 
CAAGGCAAGG AGGGCAATGG GACCATCAAC GGGGCCAGGA GTGAGCGGCG GACCCGCCAC 
CGTGGAGGGT CGAGGTCTGG GAACCGGGAA GGAGAGCCTG GGTCCAAGGG CGAGAATGGA 
GAGGAGCCTC ACAGACGGCA CCGGTTCAGG AGCAGGGCGC TGTCGACGTA CGATTCGGTG 
GAGAAGGAGA ACAGGGAGAA GGAAGGGGAG ACTGCTGAGA AGGAGCACCA GAATCATCAG 
CCCAAGGAGA ATCAGTGTGA GATTGAGGCC AGTGGGAGTG TGAGCATCCC GGTGCACACC 
CTTCCCAGCA CCTATCTGCA GAAAGTGCCA GAGCAGCCTG AAGATGCTGA CAACCAGAAG 
AACGTGACAC GCATGATTCA GCCGCCTCTG GACAAAACCA CCACTGTGAA CATCCCCGTC 
ACCATCACCG CCCCGCCAGG AGAAACCACC GTCATACCTA TGAACAACGT TGAGTTCGAA 
AGTAAAACAG AAGAGAAGAA GGACGTTGAT GACTTGACGA AAAATGGGCC TAAACCAATC 
TTGCCTTACA GTTCCATGTT CATCCTAAGT CCTACGAACC CGATCCGGCG TCTGTTCCAC 
TACATCGTCA ACCTGCGCTA CTTCGAGATG GTCATCCTCA TCGTCATCGC TCTCAGCAGC 
ATTGCCCTGG CAGCAGAAGA CCCCGTCCAA GCAGAATCAC CAAGGAACGA TGCTCTGAAG 
TACTTGGATT ATATATTCAC GGGTGTCTTT ACCTTCGAGA TGGTGATCAA GATGATTGAC 
CTGGGGCTGT TACTTCACCC TGGCTCCTAT TTCCGTGACC TGTGGAACAT CTTGGACTTC 
ATTGTGGTCA GTGGGGCCTT GGTGGCATTT GCTTTCTCAG GAACCAAAGG CAAGGACATC 
AACACGATCA AGTCGCTGCG GGTTCTGCGG GTCCTACGAC CACTGAAGAC CATTAAACGG 
CTGCCAAAAC TAAAGGCTGT CTTTGACTGC GTGGTGAATT CCCTGAAGAA CGTCCTCAAC 
ATCCTCATTG TCTACATGCT CTTCATGTTC ATCTTTGCTG TCATTGCTGT GCAGCTCTTC 
AAAGGCAGAT TCTTCTACTG CACTGATGAG TCGAAAGAGC TGGAGAAGGA TTGCAGGGGA 
CAATACCTTG ACTATGAAAA AAATGAGGTG GAGGCGCAAC CCAGGGAATG GAAAAAATAT 
GAGTTCCATT ACGACAATGT GCTGTGGGCT CTGCTGACAC TTTTCACTGT CTCCACAGGA 
GAAGGGTGGC CAACTGTGCT GAAGCACTCA GTGGATGCTA CCTACGAGGA ACAGGGTCCC 
AGCCCTGGCT ACCGAATGGA AATGTCTATC TTTTACGTGG TGTATTTTGT TGTCTTCCCT 
TTTTTCTTTG TGAACATCTT TGTGGCGTTA ATCATAATCA CCTTCCAAGA GCAAGGAGAT 
AAAGTGATGT CAGAATGCAG CCTAGAGAAA AATGAGAGGG CCTGCATTGA CTTCGCCATA 
AGTGCCAAGC CACTGACCCG CTACATGCCT CAGAACAAGC AGTCATTTCA GTACAAGATG 
TGGAAATTTG TGGTGTCCCC GCCCTTTGAG TATTTTATCA TGGTCATGAT TGCACTCAAT 
ACTATTGTTC TCATGATGAA GTTCTATGAT GCTCCAGAAG CATATGAAGA AATGTTGAAA 
TGCCTGAATA TTGTCTTTAC ATCCATGTTT TCAATGGAGT GTGTGCTGAA GATCATTGCC 
TTTGGTGTGC TGAATTATTT CCGAGATGCC TGGAATGTCT TTGATTTTGT AACAGTGTTG 
GGAAGTATTA CTGATATTTT AGTAACAGAG ATTGCGGACA ACTTCATCAA CCTCAGCTTC 
CTGCGGCTCT TCAGGGCAGC AAGACTTATC AAGCTCCTTC GCCAGGGTTA CACTATCCGC 
ATCTTGCTCT GGACGTTTGT GCAGTCATTT AAGGCCTTGC CTTATGTGTG TCTTCTCATT 
GCAATGCTCT TCTTCATCTA TGCCATTATT GGCATGCAGG TATTTGGAAA CATTGCACTA 
AATGACGAAA CCTCCATCAA TCGGCACAAT AATTTCCGTA CCTTCCTTCA AGCCTTGATG 
CTTCTGTTCA GGAGTGCCAC GGGGGAGGCC TGGCACGAGA TCATGCTGTC ATGCCTGAGC 
AACAGGGCCT GCGACCCACT CTCCGGCCTC ACCAAGAACG AGTGCGGCAG CGAGTTCGCC 
TACTTCTACT TCGTGTCATT CATCTTCCTC TGCTCCTTCC TGATGTTGAA CCTATTTGTG 
GCTGTAATCA TGGACAACTT TGAATACTTG ACAAGAGACT CCTCCATCCT TGGGCCCCAT 
CATTTGGATG AGTTTGTCCG TGTCTGGGCT GAATACGACC CAGCTGCCTG CGGGCGCATC 
AGTTACACAG ACATGTATGA GATGCTGAGA CACATGTCCC CACCTCTAGG CCTTGGAAAG 
AAATGCCCTG CTCGCGTTGC CTATAAGCGC CTGGTGCGGA TGAACATGCC CATCTCACCC 
GAGGATCTGA CAGTCCACTT CACCTCCACG CTGATGGCCC TGATCCGCAC TGCTCTGGAA 
ATCAAGCTCG CCTCAGGGGG TGTTAAGCAG CACCAGTGTG ATGCTGAGCT GAGAAAGGAG 
ATATCTCTAG TTTGGCCCAA CCTGTCCCAG AAGACACTGG ATTTGCTGGT GCCTCCTCAC 
AAACCTGATG AGATGACTGT GGGGAAGGTC TACGCAGCGC TCATGATATT TGACTTCTAC 
AAACAGAACA AGAACAGCAG GGAACAAGTC CACCAGCCTC CTGGGGGCCT GTGCCAGCCC 
GGACCAGTGT CACTCTTCCA CCCCTTGAAG GCCACTTTGG AGCAAACCCA GCCCTCGGCA 
TTCAGCAGTG CGAAGGCATT CCTGCGCCAG AAAAGCTCGG CCTCACTCAA CAATGGAGGC 
ACATTGCCAG CCCCAGAGGG TGGGATCAAA GAGTCCAGTT CGTGGGGGAC CCAACGGACC 
CAGGACGTGT TTTATGAAAC CAGGACACCA GCTTTTGAAC GAGGCCACTC AGAGGAAATC 
CCCATTGAAC GGGTGGTGGA AATGAGGGAA ATCAGCCCCA CACTTGCCAA TGGAGAGCAC 
CAACCAGGGC TGGAGAGCCA GGGTCGGGCG GCTTCCATGC CGCGCCTGGC TGCTGAAACG 
CAGCCCATCC CGGACACAAG CCCCATGAAG CGCTCCATCT CCACGCTGAC CCCGCAGCGT 
CCTCACCCCA TGCACCTCTA TGAGTACTCC CTGGAGCGCG TGCCCACGGA CCAGGTGCAT 
CACCACCACC ACCACCGCTG CCACCGGCGG AAAGAGAAGA AGCAGAAGTC CTTGGACAGG 
ACCACACATC AGCTGGCCGA TGGGGAAGCT GTGGCCCAGT CTGGTGAATC TTCTTCCAAG 
GACAAGAAGC AAGAGCGTGG ACGGTCCCAA GAGAGGAAGC AGCACTCTTC CTCCTCCTCA 
GAAAAGCAGC GCTTCTACTC CTGTGATCGC TATGGCAGCC GGGACCGATC CCAGCCCAAG 
TCTGCTGATC AGAGCAGGCC TACTTCACCC AATGGTGGGC CTGAGCAAGG TCCCCACAGA 
CAGGGCAGTG GTTCGGTTAA CGGGAGCCCC TTGCTGTCGA CATCTGGTGC TAGTACCCCC 
TGCCGGGGTC GGAGGCAGCT CCCGCAGACC CCCCTGACCC CACGCCCCAG CATCACTTAC 
AAGACCGCCA ACTCCTCGCC CGTACACTTC AGCACGTCGC CGGGCGGCCT CCCGCCCTTC 
TCCCCGGGCC GCCTGAGCCG CGGGCTGTCT GAGCACAATG CACTGCTGCG TGGGGACCAG 
CAGCCGCCCC CGGCCGCGGT TGCCCGCATC GGCTCCGACC CTTACCTGGG CCACCGCGAC 
GCCGCCGACA GCCCCATAGG CGCAGCGCCC GAGGACACGC TCACCTTCGA GGAAGCCGTG 
GCCACCAACT CGGGGCGTTC CTCCAGGACT TCTTACGTCT CCTCCTTGAC CTCCCAGTCT 
CACCAAGCAC GCCGGGTGCC CAATGGCTAC CATTACACGC TGGGGCTCAA CACGGGGCCC 
GGCACTGGCA CCAGGGGACG GAGCTACTAC CACGAAGCCG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( NM_204293.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989624.1 Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.

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CloneID	OGa26844
Clone ID Related Accession (Same CDS sequence)	NM_204293.1
Accession Version	NM_204293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Gallus gallus(chicken)
Product	voltage-dependent N-type calcium channel subunit alpha-1B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF173012.1. Sequence Note: This sequence has been modified as follows: removed 34 bp suspected to be vector contamination from the 3' end. ##Evidence-Data-START## Transcript exon combination :: AF173012.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGATTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTGC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATAAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGGTG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT ACTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAGGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAATGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTATATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG ACTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGTT GCCGGATTCA TTATAAGGAT ATGTACAATT TGTTACGTGT AATTGCTCCA 
CCCCTGGGCT TAGGAAAGAA GTGCCCTCAT AGGGTGGCAT ACAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACG GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACGCTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AGCAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ACGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCAGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GAGGAGCAAA GCACGGTCAC CTGGGAGTTA CCTAGCACCC 
ATCCCGGACA CAAGCCCCAT GAAGCGCTCC ATCTCCACGC TGACCCCGCA GCGTCCTCAC 
CCCATGCACC TCTATGAGTA CTCCCTGGAG CGCGTGCCCA CGGACCAGGT GCATCACCAC 
CACCACCACC GCTGCCACCG GCGGAAAGAG AAGAAGCAGA AGTCCTTGGA TAGGACCACA 
CATCAGCTGG CCGATGGGGA AGCTGTGGCC CAGTCTGGTG AATCTTCTTC CAAGGACAAG 
AAGCAAGAGC GCGGACGGTC CCAAGAGAGG AAGCAGCACT CTTCCTCCTC CTCAGAAAAG 
CAGCGCTTCT ACTCCTGTGA TCGCTATGGC AGCCGGGACC GATCCCAGCC CAAGTCTGCT 
GATCAGAGCA GGCCTACTTC ACCCAATGGT GGGCCTGAGC AAGGTCCCCA CAGACAGGGC 
AGTGGTTCGG TTAACGGGAG CCCCTTGCTG TCGACATCTG GTGCTAGTAC CCCCTGCCGG 
GGTCGGAGGC AGCTCCCGCA GACCCCCCTG ACCCCACGCC CCAGCATCAC TTACAAGACC 
GCCAACTCCT CGCCCGTACA CTTCAGCACG TCGCCGGGCG GCCTCCCGCC CTTCTCCCCG 
GGCCGCCTGA GCCGCGGGCT GTCTGAGCAC AATGCACTGC TGCGTGGGGA CCAGCAGCCG 
CCCCCGGCCG CGGTTGCCCG CATCGGCTCT GACCCTTACC TGGGCCACCG CGACGCCGCC 
GACAGCCCCA TAGGCGCAGC GCCCGAGGAC ACGCTCACCT TCGAGGAAGC CGTGGCCACC 
AACTCGGGGC GTTCCTCCAG GACTTCTTAC GTCTCCTCCT TGACCTCCCA GTCTCACCAA 
GCACGCCGGG TGCCCAATGG CTACCATTAC ACGCTGGGGC TCAACACGGG GCCCGGCACT 
GGCACCAGGG GACGGAGCTA CTACCACGAA GCCGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989624.1
CDS	86..7159
Translation	MARFGDDLPTRYGGGGPAGAGRGSSRQGGPQAGQRMYKQSMAQRARTMALYNPIPVKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGLEFYMGKFHKTCFSNKTGEEVGDFPCGEEPPARQCESGTTCREYWQGPNYGITNFDNILFAVLTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDGRAASEGPIQQGTAPAETSSGSSYNMLKRAAIKKSKNDLIHAEEGEDHFTDICSVGSPFARASLKSGKNESSSYFRRKEKMFRFFIRRMVKAQSFYWIVLCVVALNTLCVAMVHYDQPEKLTTALYFAEFVFLGLFLTEMSLKMYGLGPRNYFHSSFNYFDFGVIVGSIFEVIWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFRDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHSGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEATNQKLALQKAKEVAEVSPMSAANISIAAKQQNSSKSKSVWEQRTSQIRMHNFRASCEALYNELDPEERVRYATTLHIRPDMKTHLDRPLVVEPRGEGRNNISKLSPVDVQEVEQTKVSSTDGAEAPRKHHRHRDKEKLGEQEKGDVTKDENGESGINNKEERHRQHRSRSKEVEGGSKEGKSDRSRGQEGGKRHHRRGSVEEGVEKEHRRHRTHRHSAERQGKEGNGTINGARSERRTRHRGGSRSGNREGEPGSKGENGEEPHRRHRFRSRALSTYDSVEKENREKEGETAEKEHQNHQPKENQCEIEASGSVSIPVHTLPSTYLQKVPEQPEDADNQKNVTRMIQPPLDKTTTVNIPVTITAPPGETTVIPMNNVEFESKTEEKKDVDDLTKNGPKPILPYSSMFILSPTNPIRRLFHYIVNLRYFEMVILIVIALSSIALAAEDPVQAESPRNDALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGRFFYCTDESKELEKDCRGQYLDYEKNEVEAQPREWKKYEFHYDNVLWALLTLFTVSTGEGWPTVLKHSVDATYEEQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKMWKFVVSPPFEYFIMVMIALNTIVLMMKFYDAPEAYEEMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIADTDNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNDETSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNRACDPLSGLTKNECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDPAACCRIHYKDMYNLLRVIAPPLGLGKKCPHRVAYKRLVRMNMPISPEDLTVHFTSTLMALIRTALEIKLASGGVKQHQCDAELRKEISLVWPNLSQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKSSREQVHQPPGGLCQPGPVSLFHPLKATLEQTQPSAFSSAKAFLRQKSSASLNNGGTLPAPEGGIKESSSWGTQRTQDVFYETRTPAFERGHSEEIPIERVVEMREISPTLANGEHQPGLESQGRAASMPRLAAETQRSKARSPGSYLAPIPDTSPMKRSISTLTPQRPHPMHLYEYSLERVPTDQVHHHHHHRCHRRKEKKQKSLDRTTHQLADGEAVAQSGESSSKDKKQERGRSQERKQHSSSSSEKQRFYSCDRYGSRDRSQPKSADQSRPTSPNGGPEQGPHRQGSGSVNGSPLLSTSGASTPCRGRRQLPQTPLTPRPSITYKTANSSPVHFSTSPGGLPPFSPGRLSRGLSEHNALLRGDQQPPPAAVARIGSDPYLGHRDAADSPIGAAPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHQARRVPNGYHYTLGLNTGPGTGTRGRSYYHEADEDDWC

Target ORF information:

RefSeq Version	NM_204293.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 B (CACNA1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204293.1

ORF Insert Sequence:

ATGGCTCGGT TCGGGGATGA TCTACCAACA CGCTATGGAG GTGGAGGGCC GGCCGGGGCT 
GGAAGAGGGA GCAGCCGGCA AGGTGGCCCC CAAGCCGGCC AAAGGATGTA TAAGCAGTCG 
ATGGCTCAGA GGGCCCGGAC TATGGCTCTC TACAACCCCA TCCCTGTCAA ACAGAACTGC 
TTCACAGTCA ACAGATCCCT CTTCATCTTC AGTGAAGATA ATGTTATACG GAAATATGCC 
AAGAGAATAA CAGAATGGCC TCCATTTGAA TACATGATTT TAGCTACAAT CATAGCCAAT 
TGTATTGTGC TGGCTCTGGA ACAACATTTG CCGGATGGAG ACAAGACACC TATGTCAGAG 
AGATTGGATG ACACTGAGCC GTACTTTATT GGAATATTTT GTTTTGAAGC TGGTATAAAG 
ATCATCGCTC TGGGGTTTGT TTTTCACAAG GGCTCTTATC TCCGAAATGG TTGGAACGTG 
ATGGATTTTG TTGTGGTCCT CACAGGGATT CTAGCAACTG CTGGGACCGA CTTCGACCTG 
CGGACCCTGA GAGCTGTCCG TGTGCTGCGG CCGCTGAAGT TGGTGTCGGG AATCCCAAGT 
CTGCAGGTTG TCCTCAAGTC CATCATGAAG GCGATGGTGC CTCTGCTTCA GATTGGGCTG 
CTCCTCTTCT TCGCCATCGT GATGTTTGCC ATCATCGGGT TGGAGTTCTA CATGGGGAAG 
TTTCACAAAA CCTGCTTCTC TAATAAGACA GGTGAAGAGG TGGGAGATTT TCCTTGCGGA 
GAGGAGCCTC CTGCCAGGCA ATGCGAGAGT GGGACCACCT GCAGGGAGTA CTGGCAAGGG 
CCCAACTACG GCATCACCAA CTTTGACAAC ATCCTCTTTG CTGTGCTCAC CGTCTTCCAG 
TGCATCACCA TGGAGGGGTG GACCGACATC CTCTACAATA CAAACGATGC TGCAGGAAAT 
ACCTGGAACT GGCTTTACTT CATCCCTCTC ATCATCATCG GCTCTTTCTT CATGCTGAAC 
TTGGTGCTGG GTGTCCTATC AGGTGAATTT GCCAAAGAAC GAGAGAGGGT AGAAAACCGT 
CGAGCTTTCC TGAAACTCCG TAGGCAACAG CAAATAGAAC GGGAGCTAAA TGGATATTTG 
GAGTGGATCT TCAAAGCAGA GGAGGTCATG CTGGCCGAGG AAGACAAGAA CGCGGAAGAG 
AAATCTCCTC TGGACGGGAG GGCCGCCTCG GAAGGGCCGA TTCAACAAGG CACGGCACCG 
GCAGAGACTA GCAGCGGCAG CAGCTACAAC ATGTTGAAAA GAGCTGCAAT AAAGAAGAGC 
AAAAACGACC TGATTCATGC TGAAGAAGGT GAGGACCATT TCACAGACAT TTGCTCCGTT 
GGGTCTCCTT TTGCCCGTGC CAGTTTGAAA AGTGGGAAGA ATGAGAGCTC ATCCTACTTC 
AGGAGGAAAG AGAAAATGTT CCGCTTCTTC ATCCGGCGCA TGGTGAAAGC CCAGAGCTTC 
TACTGGATTG TGCTCTGCGT GGTTGCCCTC AACACGCTCT GCGTCGCGAT GGTGCACTAT 
GATCAGCCAG AGAAACTCAC CACCGCGCTG TATTTCGCCG AGTTTGTTTT TCTGGGTCTG 
TTCCTTACTG AGATGTCTTT GAAGATGTAT GGACTGGGGC CAAGAAATTA CTTTCATTCC 
TCATTCAACT ACTTTGACTT TGGGGTCATT GTGGGGAGCA TCTTTGAAGT CATCTGGGCT 
GCAGTGAAAC CTGGTACCTC GTTTGGCATC AGCGTGCTGA GAGCTCTGCG GCTGCTGAGG 
ATTTTCAAAG TCACCAAATA CTGGAACTCC CTGAGGAACC TCGTGGTCTC CTTGCTGAAC 
TCCATGAAGT CCATCATCAG TTTGCTCTTT CTGCTGTTCC TCTTCATTGT GGTGTTTGCT 
CTGCTGGGGA TGCAGCTCTT TGGAGGGCAG TTCAATTTTA GAGATGAAAC ACCAACCACG 
AACTTCGATA CTTTTCCAGC TGCCATCCTC ACGGTGTTCC AGATCCTGAC TGGGGAGGAC 
TGGAACGCGG TGATGTACCA TGGCATAGAG TCGCAGGGCG GTGTCCACTC GGGGATGTTC 
TCCTCCATTT ACTTCATCGT CCTGACGTTG TTTGGTAACT ATACACTCCT GAACGTCTTC 
TTGGCCATCG CCGTTGACAA TCTGGCCAAT GCCCAGGAGC TGACCAAGGA CGAGGAGGAG 
ATGGAGGAAG CCACCAATCA GAAGCTCGCC CTGCAGAAGG CCAAGGAGGT AGCAGAAGTC 
AGCCCCATGT CTGCAGCAAA CATTTCCATA GCCGCCAAGC AGCAGAACTC CTCCAAATCC 
AAGTCGGTTT GGGAGCAACG AACCAGCCAG ATCCGGATGC ACAACTTCCG AGCCAGCTGC 
GAGGCTCTGT ACAATGAACT GGACCCCGAG GAGCGGGTGC GCTACGCCAC GACCCTACAC 
ATCCGTCCAG ACATGAAGAC CCACTTGGAT CGGCCACTGG TGGTAGAGCC AAGGGGTGAA 
GGGAGGAACA ACATCAGTAA GCTGAGCCCT GTGGATGTGC AGGAGGTGGA GCAGACCAAA 
GTCAGCTCTA CCGACGGAGC AGAAGCTCCG CGGAAGCATC ACCGGCATCG GGATAAGGAG 
AAACTGGGAG AGCAAGAGAA GGGTGATGTG ACCAAAGATG AGAATGGGGA ATCTGGCATC 
AACAATAAGG AGGAGAGGCA CAGGCAGCAC AGAAGCCGTA GCAAGGAGGT GGAAGGGGGC 
AGCAAGGAAG GGAAAAGTGA CCGCAGCAGG GGCCAGGAAG GAGGGAAGAG GCACCATCGC 
CGGGGGTCGG TGGAAGAAGG CGTTGAGAAG GAGCACCGGA GGCACCGCAC GCACCGGCAC 
TCTGCAGAAC GGCAAGGCAA GGAGGGCAAT GGGACCATCA ACGGGGCCAG GAGTGAGCGG 
CGGACCCGCC ACCGTGGAGG GTCGAGGTCT GGGAACCGGG AAGGAGAGCC TGGGTCCAAG 
GGCGAGAATG GAGAGGAGCC TCACAGACGG CACCGGTTCA GGAGCAGGGC GCTGTCGACG 
TACGATTCGG TGGAGAAGGA GAACAGGGAG AAGGAAGGGG AGACTGCTGA GAAGGAGCAC 
CAGAATCATC AGCCCAAGGA GAATCAGTGT GAGATTGAGG CCAGTGGGAG TGTGAGCATC 
CCGGTGCACA CCCTTCCCAG CACCTATCTG CAGAAAGTGC CAGAGCAGCC TGAAGATGCT 
GACAACCAGA AGAACGTGAC ACGCATGATT CAGCCGCCTC TGGACAAAAC CACCACTGTG 
AACATCCCCG TCACCATCAC CGCCCCGCCA GGAGAAACCA CCGTCATACC TATGAACAAC 
GTTGAGTTCG AAAGTAAAAC AGAAGAGAAG AAGGACGTTG ATGACTTGAC GAAAAATGGG 
CCTAAACCAA TCTTGCCTTA CAGTTCCATG TTCATCCTAA GTCCTACGAA CCCGATCCGG 
CGTCTGTTCC ACTACATCGT CAACCTGCGC TACTTCGAGA TGGTCATCCT CATCGTCATC 
GCTCTCAGCA GCATTGCCCT GGCAGCAGAA GACCCCGTCC AAGCAGAATC ACCAAGGAAC 
GATGCTCTGA AGTACTTGGA TTATATATTC ACGGGTGTCT TTACCTTCGA GATGGTGATC 
AAGATGATTG ACCTGGGGCT GTTACTTCAC CCTGGCTCCT ATTTCCGTGA CCTGTGGAAC 
ATCTTGGACT TCATTGTGGT CAGTGGGGCC TTGGTGGCAT TTGCTTTCTC AGGAACCAAA 
GGCAAGGACA TCAACACGAT CAAGTCGCTG CGGGTTCTGC GGGTCCTACG ACCACTGAAG 
ACCATTAAAC GGCTGCCAAA ACTAAAGGCT GTCTTTGACT GCGTGGTGAA TTCCCTGAAG 
AACGTCCTCA ACATCCTCAT TGTCTATATG CTCTTCATGT TCATCTTTGC TGTCATTGCT 
GTGCAGCTCT TCAAAGGCAG ATTCTTCTAC TGCACTGATG AGTCGAAAGA GCTGGAGAAG 
GATTGCAGGG GACAATACCT TGACTATGAA AAAAATGAGG TGGAGGCGCA ACCCAGGGAA 
TGGAAAAAAT ATGAGTTCCA TTACGACAAT GTGCTGTGGG CTCTGCTGAC ACTTTTCACT 
GTCTCCACAG GAGAAGGGTG GCCAACTGTG CTGAAGCACT CAGTGGATGC TACCTACGAG 
GAACAGGGTC CCAGCCCTGG CTACCGAATG GAAATGTCTA TCTTTTACGT GGTGTATTTT 
GTTGTCTTCC CTTTTTTCTT TGTGAACATC TTTGTGGCGT TAATCATAAT CACCTTCCAA 
GAGCAAGGAG ATAAAGTGAT GTCAGAATGC AGCCTAGAGA AAAATGAGAG GGCCTGCATT 
GACTTCGCCA TAAGTGCCAA GCCACTGACC CGCTACATGC CTCAGAACAA GCAGTCATTT 
CAGTACAAGA TGTGGAAATT TGTGGTGTCC CCGCCCTTTG AGTATTTTAT CATGGTCATG 
ATTGCACTCA ATACTATTGT TCTCATGATG AAGTTCTATG ATGCTCCAGA AGCATATGAA 
GAAATGTTGA AATGCCTGAA TATTGTCTTT ACATCCATGT TTTCAATGGA GTGTGTGCTG 
AAGATCATTG CCTTTGGTGT GCTGAATTAT TTCCGAGATG CCTGGAATGT CTTTGATTTT 
GTAACAGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA CACGGACAAC 
TTCATCAACC TCAGCTTCCT GCGGCTCTTC AGGGCAGCAA GACTTATCAA GCTCCTTCGC 
CAGGGTTACA CTATCCGCAT CTTGCTCTGG ACGTTTGTGC AGTCATTTAA GGCCTTGCCT 
TATGTGTGTC TTCTCATTGC AATGCTCTTC TTCATCTATG CCATTATTGG CATGCAGGTA 
TTTGGAAACA TTGCACTAAA TGACGAAACC TCCATCAATC GGCACAATAA TTTCCGTACC 
TTCCTTCAAG CCTTGATGCT TCTGTTCAGG AGTGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCAT GCCTGAGCAA CAGGGCCTGC GACCCACTCT CCGGCCTCAC CAAGAACGAG 
TGCGGCAGCG ACTTCGCCTA CTTCTACTTC GTGTCATTCA TCTTCCTCTG CTCCTTCCTG 
ATGTTGAACC TATTTGTGGC TGTAATCATG GACAACTTTG AATACTTGAC AAGAGACTCC 
TCCATCCTTG GGCCCCATCA TTTGGATGAG TTTGTCCGTG TCTGGGCTGA ATACGACCCA 
GCTGCCTGTT GCCGGATTCA TTATAAGGAT ATGTACAATT TGTTACGTGT AATTGCTCCA 
CCCCTGGGCT TAGGAAAGAA GTGCCCTCAT AGGGTGGCAT ACAAGCGCCT GGTGCGGATG 
AACATGCCCA TCTCACCCGA GGATCTGACG GTCCACTTCA CCTCCACGCT GATGGCCCTG 
ATCCGCACTG CTCTGGAAAT CAAGCTCGCC TCAGGGGGTG TTAAGCAGCA CCAGTGTGAT 
GCTGAGCTGA GAAAGGAGAT ATCTCTAGTT TGGCCCAACC TGTCCCAGAA GACGCTGGAT 
TTGCTGGTGC CTCCTCACAA ACCTGATGAG ATGACTGTGG GGAAGGTCTA CGCAGCGCTC 
ATGATATTTG ACTTCTACAA ACAGAACAAG AGCAGCAGGG AACAAGTCCA CCAGCCTCCT 
GGGGGCCTGT GCCAGCCCGG ACCAGTGTCA CTCTTCCACC CCTTGAAGGC CACTTTGGAG 
CAAACCCAGC CCTCGGCATT CAGCAGTGCG AAGGCATTCC TGCGCCAGAA AAGCTCGGCC 
TCACTCAACA ACGGAGGCAC ATTGCCAGCC CCAGAGGGTG GGATCAAAGA GTCCAGTTCG 
TGGGGGACCC AACGGACCCA GGACGTGTTT TATGAAACCA GGACACCAGC TTTTGAACGA 
GGCCACTCAG AGGAAATCCC CATTGAACGG GTGGTGGAAA TGAGGGAAAT CAGCCCCACA 
CTTGCCAATG GAGAGCACCA ACCAGGGCTG GAGAGCCAGG GTCGGGCAGC TTCCATGCCG 
CGCCTGGCTG CTGAAACGCA GAGGAGCAAA GCACGGTCAC CTGGGAGTTA CCTAGCACCC 
ATCCCGGACA CAAGCCCCAT GAAGCGCTCC ATCTCCACGC TGACCCCGCA GCGTCCTCAC 
CCCATGCACC TCTATGAGTA CTCCCTGGAG CGCGTGCCCA CGGACCAGGT GCATCACCAC 
CACCACCACC GCTGCCACCG GCGGAAAGAG AAGAAGCAGA AGTCCTTGGA TAGGACCACA 
CATCAGCTGG CCGATGGGGA AGCTGTGGCC CAGTCTGGTG AATCTTCTTC CAAGGACAAG 
AAGCAAGAGC GCGGACGGTC CCAAGAGAGG AAGCAGCACT CTTCCTCCTC CTCAGAAAAG 
CAGCGCTTCT ACTCCTGTGA TCGCTATGGC AGCCGGGACC GATCCCAGCC CAAGTCTGCT 
GATCAGAGCA GGCCTACTTC ACCCAATGGT GGGCCTGAGC AAGGTCCCCA CAGACAGGGC 
AGTGGTTCGG TTAACGGGAG CCCCTTGCTG TCGACATCTG GTGCTAGTAC CCCCTGCCGG 
GGTCGGAGGC AGCTCCCGCA GACCCCCCTG ACCCCACGCC CCAGCATCAC TTACAAGACC 
GCCAACTCCT CGCCCGTACA CTTCAGCACG TCGCCGGGCG GCCTCCCGCC CTTCTCCCCG 
GGCCGCCTGA GCCGCGGGCT GTCTGAGCAC AATGCACTGC TGCGTGGGGA CCAGCAGCCG 
CCCCCGGCCG CGGTTGCCCG CATCGGCTCT GACCCTTACC TGGGCCACCG CGACGCCGCC 
GACAGCCCCA TAGGCGCAGC GCCCGAGGAC ACGCTCACCT TCGAGGAAGC CGTGGCCACC 
AACTCGGGGC GTTCCTCCAG GACTTCTTAC GTCTCCTCCT TGACCTCCCA GTCTCACCAA 
GCACGCCGGG TGCCCAATGG CTACCATTAC ACGCTGGGGC TCAACACGGG GCCCGGCACT 
GGCACCAGGG GACGGAGCTA CTACCACGAA GCCGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Long C terminal splice variant CaV2.2 identified in presynaptic membrane by mass spectrometric analysis.
Channels (Austin, Tex.)4(1)58-62(2010 Jan-Feb)
Gardezi SR,Taylor P,Stanley EF

Munc18: a presynaptic transmitter release site N type (CaV2.2) calcium channel interacting protein.
Channels (Austin, Tex.)1(1)11-20(2007 Jan-Feb)
Chan AW,Khanna R,Li Q,Stanley EF

RGS12 interacts with the SNARE-binding region of the Cav2.2 calcium channel.
The Journal of biological chemistry280(2)1521-8(2005 Jan)
Richman RW,Strock J,Hains MD,Cabanilla NJ,Lau KK,Siderovski DP,Divers?-Pierluissi M

Cloning and functional expression of novel N-type Ca(2+) channel variants.
The Journal of biological chemistry274(49)34566-75(1999 Dec)
L? Q,Dunlap K

Predominance of the alpha1D subunit in L-type voltage-gated Ca2+ channels of hair cells in the chicken's cochlea.
Proceedings of the National Academy of Sciences of the United States of America94(26)14883-8(1997 Dec)
Kollmar R,Montgomery LG,Fak J,Henry LJ,Hudspeth AJ

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CACNA1B cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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