cacna1ba cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1ba
Entrez Gene ID	555941
Full Name	calcium channel, voltage-dependent, N type, alpha 1B subunit, a
Synonyms	si:ch211-238e12.1
General protein information	Preferred Names calcium channel, voltage-dependent, N type, alpha 1B subunit, a Names voltage-dependent N-type calcium channel subunit alpha-1B CACNA1Bb cacna1b calcium channel, voltage-dependent, L type, alpha 1B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021476178.1	XP_021331853.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
XM_009301501.3	XP_009299776.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
XM_021476180.1	XP_021331855.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
XM_009301504.3	XP_009299779.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
XM_009301493.2	XP_009299768.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_009301494.2	XP_009299769.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_009301496.2	XP_009299771.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
XM_009301498.2	XP_009299773.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
XM_009301504.2	XP_009299779.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
XM_009301505.2	XP_009299780.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
XM_009301497.2	XP_009299772.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
XM_009301499.2	XP_009299774.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
XM_017356095.1	XP_017211584.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
XM_017356097.1	XP_017211586.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
NM_001114548.1	NP_001108020.1	voltage-dependent N-type calcium channel subunit alpha-1B
XM_021476176.1	XP_021331851.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
XM_017356095.2	XP_017211584.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
XM_009301495.2	XP_009299770.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_009301497.3	XP_009299772.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_021476175.1	XP_021331850.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_017356096.2	XP_017211585.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
XM_021476182.1	XP_021331857.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
XM_009301492.2	XP_009299767.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_009301501.2	XP_009299776.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
XM_021476179.1	XP_021331854.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
XM_009301505.3	XP_009299780.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
XM_021476181.1	XP_021331856.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
XM_009301500.2	XP_009299775.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
XM_017356096.1	XP_017211585.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
XM_009301503.2	XP_009299778.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
XM_021476173.1	XP_021331848.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_021476174.1	XP_021331849.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_009301500.3	XP_009299775.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
XM_017356097.2	XP_017211586.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CACNA1B	XP_520396.4
Canis lupus familiaris (dog)	CACNA1B	XP_005625483.1
Rattus norvegicus (Norway rat)	Cacna1b	NP_671482.1
Danio rerio (zebrafish)	cacna1ba	NP_001108020.1
Caenorhabditis elegans (roundworm)	unc-2	NP_001123176.1
Gallus gallus (chicken)	CACNA1B	NP_989624.1
Drosophila melanogaster (fruit fly)	cac	NP_001245636.1
Homo sapiens (human)	CACNA1B	NP_000709.1
Bos taurus (cattle)	CACNA1B	NP_777057.1
Mus musculus (house mouse)	Cacna1b	NP_001035993.1
Danio rerio (zebrafish)	cacna1bb	XP_002665969.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002578	XP_312358.4

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Rabinowitz JS, Robitaille AM, Wang Y, Ray CA, Thummel R, Gu H, Djukovic D, Raftery D, Berndt JD, Moon RT
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 Jan)

Maternal Rest/Nrsf Regulates Zebrafish Behavior through snap25a/b.
Moravec CE, Samuel J, Weng W, Wood IC, Sirotkin HI
The Journal of neuroscience : the official journal of the Society for Neuroscience36(36)9407-19(2016 09)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cacna1ba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1ba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142639	XM_021476173.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142640	XM_021476174.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142641	XM_021476175.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142642	XM_021476176.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142643	XM_021476178.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142644	XM_021476179.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142645	XM_021476180.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142646	XM_021476181.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142647	XM_021476182.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59085	XM_017356095.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59086	XM_017356096.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59087	XM_017356097.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa36125	NM_001114548.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa42256	XM_009301492.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42257	XM_009301493.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42258	XM_009301494.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42259	XM_009301495.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42260	XM_009301496.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42261	XM_009301497.3 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42262	XM_009301498.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42263	XM_009301499.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42264	XM_009301500.3 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42265	XM_009301501.3 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42266	XM_009301503.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa42267	XM_009301504.3 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa42268	XM_009301505.3 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
ODa42267	XM_009301504.2	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa59085	XM_017356095.1	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa42261	XM_009301497.2	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42265	XM_009301501.2	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42268	XM_009301505.2	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa59086	XM_017356096.1	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa42264	XM_009301500.2	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa59087	XM_017356097.1	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1ba ( XM_021476173.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331848.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa142639
Clone ID Related Accession (Same CDS sequence)	XM_021476173.1
Accession Version	XM_021476173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC 
AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC 
ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT 
CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT 
GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT 
CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT 
CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC 
TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG 
TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC 
CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGCGGA 
CGGATTTCCT ATAAGGATAT GTACAATTTG TTGCGAATTA TCTCGCCCCC CCTTGGCTTA 
GGGAAGAACT GCCCTAATAG GGTAGCATAC AAGCGTCTGG TGAAAATGAA CATGCCCATT 
GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG 
GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG 
GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA 
CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT 
TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA 
GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC 
ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA 
CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT 
TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT 
AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA 
AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA 
GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA 
ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA 
CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC 
CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG 
AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT 
GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA 
GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC 
AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC 
AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT 
CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC 
TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC 
CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC 
TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC 
CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC 
CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT 
CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC 
AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331848.1
CDS	1..7137
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYKDMYNLLRIISPPLGLGKNCPNRVAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_021476173.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476173.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC 
AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC 
ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT 
CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT 
GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT 
CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT 
CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC 
TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG 
TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC 
CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGCGGA 
CGGATTTCCT ATAAGGATAT GTACAATTTG TTGCGAATTA TCTCGCCCCC CCTTGGCTTA 
GGGAAGAACT GCCCTAATAG GGTAGCATAC AAGCGTCTGG TGAAAATGAA CATGCCCATT 
GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG 
GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG 
GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA 
CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT 
TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA 
GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC 
ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA 
CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT 
TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT 
AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA 
AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA 
GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA 
ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA 
CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC 
CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG 
AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT 
GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA 
GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC 
AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC 
AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT 
CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC 
TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC 
CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC 
TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC 
CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC 
CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT 
CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC 
AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_021476174.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331849.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA.

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CloneID	ODa142640
Clone ID Related Accession (Same CDS sequence)	XM_021476174.1
Accession Version	XM_021476174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACGAGC GTCAGCTGAA CTTCAGTTTC 
CTGAGGCTCT TTAGAGCAGC CCGACTCATC AAGCTCCTCC GGCAGGGATA CACCATTCGC 
ATCCTGCTCT GGACATTTGT CCAGTCTTTT AAGGCACTGC CTTATGTTTG CCTTCTAATC 
GCCATGCTGT TTTTCATCTA CGCCATTATT GGGATGCAGG TGTTTGGAAA CATTGATTTG 
AATGATGACA CGGCAATCAA CCGTCACAAC AACTTCCGCA CTTTCCTCCA AGCTCTTATG 
CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT TGGCATGATA TCATGCTGTC GTGTCTGAGC 
GAGCGCACCT GTGATCCGAG CTCAGGCACC CTTGGAAAGG AGTGTGGGAG TGACTTTGCC 
TACTTCTACT TTGTCTCCTT CATTTTCCTC TGCTCATTTC TGATGTTGAA CCTGTTTGTG 
GCCGTCATTA TGGACAACTT TGAATATCTG ACACGCGACT CCTCCATCCT GGGCCCTCAC 
CACCTGGATG AGTTTATTCG AGTGTGGGCG GAGTATGACC CAGCCGCATG TGGCCGTATC 
AAGTACCTCG ATATGTATGA GATGCTGCTC CACATGTCCC CACCTTTAGG TTTGGGAAAG 
AAATGTCCTC CACGAATCGC CTACAAGCGT CTGGTGAAAA TGAACATGCC CATTGCGGAT 
GACAACAGTG TACACTTTAC CTCAACGCTG ATGGCTCTGA TCCGTACTGC CCTGGAGATC 
AAACTGGCTT CAGGCATGGT GGCTCAGCGT CTGTCAGATG CTGAACTGAA GAAGGAGCTC 
TCTACAGTTT GGCCAAACCT GTCTCAGAAG ACCATGGACC TGCTGGTCAC TCCACATAAA 
CCTAATGAGC TCACAGTGGG GAAGGTGTAT GCAGCTCTTA TGATCTTTGA CTATTACAAG 
CAGAACAGAG CAAAAAGACT TCAGATGCAG CAACAGCAGC TGAAAGAACA GGCAGGACCT 
GGATCTCAGA ATAAAGTCAG CGCTCTCTTA GAGCCCATTC TCCCACTAAC CCACATGCAA 
GATCAGCCAG TGAACGGTGT CGATTCAGCT TCAGAGTGTC AAGCTCAGAC CAGACCCAGC 
TCCACCACAC TCAACAATGG ACGCACAACT CTTGACAACA CAATAAAAGG GTCTTCATCC 
TGGGCCTCAG AGAAATCCAA GGAAGTCCAA AAGTCTAAAC GGAGACCAAT GTCTAGAGGT 
CAATCTGAGG ATGCCTCAAA TGCAAACACT GCCCAGGAGT CAGTAGAAAT GAGAAAGATG 
GAGAACAGTG CCAACACAGT GACCTCTGGT GGCCTTGAGG GCCAAGGACG TGCAGCGTCC 
ATGCCAAAAC TCAATGCTGA GATGCAGAGG AGTCATTCCC GTCAATCTCC TGGAACATTA 
CTGGAACCCA TTCCAGATTC CCCCATGAGG CGCTCTGCAT CCACTTTTGC ACCACAGCGC 
CCTCACGAGG TGAATCTGAA TGACTACATT CTGGAGAAAC CTGTTCAGGA ACGCCATCAC 
CACCATCACC ATCATCACCG ATGCCATCAT CGCCGGGACA GAGACAAGAA ACAGAGATCT 
CTGGACAGGT CGCCTACTGG CCATCACAAA ACTACAGGCA CAGCCACTGA CTCTGTAGAG 
GAGAGGGCAC ATGACCGGGG TCGTTCCCAT GAGAGGAAGC ACCATTCATC TTCAGGAGAG 
AAGCAGCGGT ATTACTCGTG TGATCGCTAT GCCAGTCGGG AACACTGTTC CAGCAAATCT 
GCCGTGGCAA GCTGTGCCAC CTCACCCAGC GAGACCCAGG AGGCCAATCT TAACAAGCAG 
GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG TTGTTGACTT CAGGGGCGAG CACTCCAAGC 
CGCGGGCGCA GACAGCTGCC CCAGACCCCC CTCACTCCTC GTCCCTCCGT GGCCTACAGG 
ACTGCCAACT CTTCCCCTGT GCAACTTCTG GCCACAACGA CCAGCTTGCT TCCCCCGAGT 
CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG CACAACGCTC TTTTACTCAG CGGCTCTATC 
TCCAGCTCGC CGGCCCCCGT CAGTCGCATT AACTCAGAAC CCTTCTTGGG GCCCCCGGAC 
CACCCTGCTG GCTTGCAGCC CGAAGCCTTC GCTGTCTTCC AAGAGAATCC GAGCCCTCAG 
ATGGGCCGTT CTCCTCAGAC TGTAGGGATG CCCTTCGTGG CGATCCCCCC ACCTCCCCCA 
CAGCAGTTTC GCAGGGTCCC CAACGGGTAC CACTTCTCCA CGGGCCCCAG CGCCAGCAGA 
GGACGGGCAC AGCAGTACTA CCCAGAGGCA GAGGTGGAGG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331849.1
CDS	1..7131
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_021476174.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476174.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACGAGC GTCAGCTGAA CTTCAGTTTC 
CTGAGGCTCT TTAGAGCAGC CCGACTCATC AAGCTCCTCC GGCAGGGATA CACCATTCGC 
ATCCTGCTCT GGACATTTGT CCAGTCTTTT AAGGCACTGC CTTATGTTTG CCTTCTAATC 
GCCATGCTGT TTTTCATCTA CGCCATTATT GGGATGCAGG TGTTTGGAAA CATTGATTTG 
AATGATGACA CGGCAATCAA CCGTCACAAC AACTTCCGCA CTTTCCTCCA AGCTCTTATG 
CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT TGGCATGATA TCATGCTGTC GTGTCTGAGC 
GAGCGCACCT GTGATCCGAG CTCAGGCACC CTTGGAAAGG AGTGTGGGAG TGACTTTGCC 
TACTTCTACT TTGTCTCCTT CATTTTCCTC TGCTCATTTC TGATGTTGAA CCTGTTTGTG 
GCCGTCATTA TGGACAACTT TGAATATCTG ACACGCGACT CCTCCATCCT GGGCCCTCAC 
CACCTGGATG AGTTTATTCG AGTGTGGGCG GAGTATGACC CAGCCGCATG TGGCCGTATC 
AAGTACCTCG ATATGTATGA GATGCTGCTC CACATGTCCC CACCTTTAGG TTTGGGAAAG 
AAATGTCCTC CACGAATCGC CTACAAGCGT CTGGTGAAAA TGAACATGCC CATTGCGGAT 
GACAACAGTG TACACTTTAC CTCAACGCTG ATGGCTCTGA TCCGTACTGC CCTGGAGATC 
AAACTGGCTT CAGGCATGGT GGCTCAGCGT CTGTCAGATG CTGAACTGAA GAAGGAGCTC 
TCTACAGTTT GGCCAAACCT GTCTCAGAAG ACCATGGACC TGCTGGTCAC TCCACATAAA 
CCTAATGAGC TCACAGTGGG GAAGGTGTAT GCAGCTCTTA TGATCTTTGA CTATTACAAG 
CAGAACAGAG CAAAAAGACT TCAGATGCAG CAACAGCAGC TGAAAGAACA GGCAGGACCT 
GGATCTCAGA ATAAAGTCAG CGCTCTCTTA GAGCCCATTC TCCCACTAAC CCACATGCAA 
GATCAGCCAG TGAACGGTGT CGATTCAGCT TCAGAGTGTC AAGCTCAGAC CAGACCCAGC 
TCCACCACAC TCAACAATGG ACGCACAACT CTTGACAACA CAATAAAAGG GTCTTCATCC 
TGGGCCTCAG AGAAATCCAA GGAAGTCCAA AAGTCTAAAC GGAGACCAAT GTCTAGAGGT 
CAATCTGAGG ATGCCTCAAA TGCAAACACT GCCCAGGAGT CAGTAGAAAT GAGAAAGATG 
GAGAACAGTG CCAACACAGT GACCTCTGGT GGCCTTGAGG GCCAAGGACG TGCAGCGTCC 
ATGCCAAAAC TCAATGCTGA GATGCAGAGG AGTCATTCCC GTCAATCTCC TGGAACATTA 
CTGGAACCCA TTCCAGATTC CCCCATGAGG CGCTCTGCAT CCACTTTTGC ACCACAGCGC 
CCTCACGAGG TGAATCTGAA TGACTACATT CTGGAGAAAC CTGTTCAGGA ACGCCATCAC 
CACCATCACC ATCATCACCG ATGCCATCAT CGCCGGGACA GAGACAAGAA ACAGAGATCT 
CTGGACAGGT CGCCTACTGG CCATCACAAA ACTACAGGCA CAGCCACTGA CTCTGTAGAG 
GAGAGGGCAC ATGACCGGGG TCGTTCCCAT GAGAGGAAGC ACCATTCATC TTCAGGAGAG 
AAGCAGCGGT ATTACTCGTG TGATCGCTAT GCCAGTCGGG AACACTGTTC CAGCAAATCT 
GCCGTGGCAA GCTGTGCCAC CTCACCCAGC GAGACCCAGG AGGCCAATCT TAACAAGCAG 
GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG TTGTTGACTT CAGGGGCGAG CACTCCAAGC 
CGCGGGCGCA GACAGCTGCC CCAGACCCCC CTCACTCCTC GTCCCTCCGT GGCCTACAGG 
ACTGCCAACT CTTCCCCTGT GCAACTTCTG GCCACAACGA CCAGCTTGCT TCCCCCGAGT 
CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG CACAACGCTC TTTTACTCAG CGGCTCTATC 
TCCAGCTCGC CGGCCCCCGT CAGTCGCATT AACTCAGAAC CCTTCTTGGG GCCCCCGGAC 
CACCCTGCTG GCTTGCAGCC CGAAGCCTTC GCTGTCTTCC AAGAGAATCC GAGCCCTCAG 
ATGGGCCGTT CTCCTCAGAC TGTAGGGATG CCCTTCGTGG CGATCCCCCC ACCTCCCCCA 
CAGCAGTTTC GCAGGGTCCC CAACGGGTAC CACTTCTCCA CGGGCCCCAG CGCCAGCAGA 
GGACGGGCAC AGCAGTACTA CCCAGAGGCA GAGGTGGAGG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_021476175.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331850.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA.

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CloneID	ODa142641
Clone ID Related Accession (Same CDS sequence)	XM_021476175.1
Accession Version	XM_021476175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCATCAC ATCAAAGCGT AAACAGAGGA ACCAAGGGCA AAGACATCAG CACTATCAAG 
TCACTGAGGG TGTTGCGAGT CTTGCGACCT CTGAAGACCA TCAAACGTCT ACCAAAACTG 
AAGGCCGTGT TTGATTGTGT GGTGAACTCC CTGAAGAATG TCCTGAATAT CCTCATTGTC 
TACATTCTGT TCATGTTCAT TTTCGCTGTC ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT 
TTTTACTGCA CAGACGAGTC CAAGGGCCTG GAGAAAGACT GCAGGGGGCT GTTTTTGGAT 
TATGATAAGG ATGATGTGAC GGCACAACCG AGGGAATGGA AGAAATACGA GTTTCACTAT 
GACAATGTGC TTTGGGCTTT TCTCACTCTC TTCACTGTTT CTACGGGAGA GGGCTGGCCT 
ACGGTTTTGA AGCACTCCGT GGATGCCACT TTTGAGGATC AGGGTCCCAG TCCAGGCTAC 
CGCATTGAGA TGTCTATTTT TTACGTGGTC TACTTTGTGG TTTTCCCTTT CTTCTTCGTC 
AACATTTTTG TAGCCTTGAT AATCATCACA TTCCAGGAAC AGGGTGATAA GGTCTTGTCA 
GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC TGCATCGACT TTGCTATCAA TGCCAAACCT 
CTGACACGCT ACATGCCGCA GAACCAGCAG TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG 
GTCTCTCCGC CGTTTGAGTA CTCCATCATG ATCATGATTG CTCTCAACAC TGTGGTGCTC 
ATGATGAAGT TCCATGGGGC GCCTGACTTT TATGAGGCAA TGCTTAAGTA TCTCAACATT 
GTCTTCACTG TTCTCTTTTC ACTGGAGTGT ATTCTCAAGA TTATTGCTTT CGGCCCCTTG 
AATTACCTGA AAGATGCCTG GAATGTGTTT GACTTTGTGA CTGTGCTGGG CAGCATCACT 
GACATTTTAG TCACTGAGAT TAACACAACG GAGCGTCAGC TGAACTTCAG TTTCCTGAGG 
CTCTTTAGAG CAGCCCGACT CATCAAGCTC CTCCGGCAGG GATACACCAT TCGCATCCTG 
CTCTGGACAT TTGTCCAGTC TTTTAAGGCA CTGCCTTATG TTTGCCTTCT AATCGCCATG 
CTGTTTTTCA TCTACGCCAT TATTGGGATG CAGGTGTTTG GAAACATTGA TTTGAATGAT 
GACACGGCAA TCAACCGTCA CAACAACTTC CGCACTTTCC TCCAAGCTCT TATGCTTTTG 
TTCAGGAGTG CCACTGGCGA GGCTTGGCAT GATATCATGC TGTCGTGTCT GAGCGAGCGC 
ACCTGTGATC CGAGCTCAGG CACCCTTGGA AAGGAGTGTG GGAGTGACTT TGCCTACTTC 
TACTTTGTCT CCTTCATTTT CCTCTGCTCA TTTCTGATGT TGAACCTGTT TGTGGCCGTC 
ATTATGGACA ACTTTGAATA TCTGACACGC GACTCCTCCA TCCTGGGCCC TCACCACCTG 
GATGAGTTTA TTCGAGTGTG GGCGGAGTAT GACCCAGCCG CATGTGGCCG TATCAAGTAC 
CTCGATATGT ATGAGATGCT GCTCCACATG TCCCCACCTT TAGGTTTGGG AAAGAAATGT 
CCTCCACGAA TCGCCTACAA GCGTCTGGTG AAAATGAACA TGCCCATTGC GGATGACAAC 
AGTGTACACT TTACCTCAAC GCTGATGGCT CTGATCCGTA CTGCCCTGGA GATCAAACTG 
GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA GATGCTGAAC TGAAGAAGGA GCTCTCTACA 
GTTTGGCCAA ACCTGTCTCA GAAGACCATG GACCTGCTGG TCACTCCACA TAAACCTAAT 
GAGCTCACAG TGGGGAAGGT GTATGCAGCT CTTATGATCT TTGACTATTA CAAGCAGAAC 
AGAGCAAAAA GACTTCAGAT GCAGCAACAG CAGCTGAAAG AACAGGCAGG ACCTGGATCT 
CAGAATAAAG TCAGCGCTCT CTTAGAGCCC ATTCTCCCAC TAACCCACAT GCAAGATCAG 
CCAGTGAACG GTGTCGATTC AGCTTCAGAG TGTCAAGCTC AGACCAGACC CAGCTCCACC 
ACACTCAACA ATGGACGCAC AACTCTTGAC AACACAATAA AAGGGTCTTC ATCCTGGGCC 
TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT AAACGGAGAC CAATGTCTAG AGGTCAATCT 
GAGGATGCCT CAAATGCAAA CACTGCCCAG GAGTCAGTAG AAATGAGAAA GATGGAGAAC 
AGTGCCAACA CAGTGACCTC TGGTGGCCTT GAGGGCCAAG GACGTGCAGC GTCCATGCCA 
AAACTCAATG CTGAGATGCA GAGGAGTCAT TCCCGTCAAT CTCCTGGAAC ATTACTGGAA 
CCCATTCCAG ATTCCCCCAT GAGGCGCTCT GCATCCACTT TTGCACCACA GCGCCCTCAC 
GAGGTGAATC TGAATGACTA CATTCTGGAG AAACCTGTTC AGGAACGCCA TCACCACCAT 
CACCATCATC ACCGATGCCA TCATCGCCGG GACAGAGACA AGAAACAGAG ATCTCTGGAC 
AGGTCGCCTA CTGGCCATCA CAAAACTACA GGCACAGCCA CTGACTCTGT AGAGGAGAGG 
GCACATGACC GGGGTCGTTC CCATGAGAGG AAGCACCATT CATCTTCAGG AGAGAAGCAG 
CGGTATTACT CGTGTGATCG CTATGCCAGT CGGGAACACT GTTCCAGCAA ATCTGCCGTG 
GCAAGCTGTG CCACCTCACC CAGCGAGACC CAGGAGGCCA ATCTTAACAA GCAGGGCAGT 
GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG ACTTCAGGGG CGAGCACTCC AAGCCGCGGG 
CGCAGACAGC TGCCCCAGAC CCCCCTCACT CCTCGTCCCT CCGTGGCCTA CAGGACTGCC 
AACTCTTCCC CTGTGCAACT TCTGGCCACA ACGACCAGCT TGCTTCCCCC GAGTCCCGGC 
AGGCTGAGCC GTGGTCTGTC CGAGCACAAC GCTCTTTTAC TCAGCGGCTC TATCTCCAGC 
TCGCCGGCCC CCGTCAGTCG CATTAACTCA GAACCCTTCT TGGGGCCCCC GGACCACCCT 
GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC TTCCAAGAGA ATCCGAGCCC TCAGATGGGC 
CGTTCTCCTC AGACTGTAGG GATGCCCTTC GTGGCGATCC CCCCACCTCC CCCACAGCAG 
TTTCGCAGGG TCCCCAACGG GTACCACTTC TCCACGGGCC CCAGCGCCAG CAGAGGACGG 
GCACAGCAGT ACTACCCAGA GGCAGAGGTG GAGGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331850.1
CDS	1..7125
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_021476175.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476175.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCATCAC ATCAAAGCGT AAACAGAGGA ACCAAGGGCA AAGACATCAG CACTATCAAG 
TCACTGAGGG TGTTGCGAGT CTTGCGACCT CTGAAGACCA TCAAACGTCT ACCAAAACTG 
AAGGCCGTGT TTGATTGTGT GGTGAACTCC CTGAAGAATG TCCTGAATAT CCTCATTGTC 
TACATTCTGT TCATGTTCAT TTTCGCTGTC ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT 
TTTTACTGCA CAGACGAGTC CAAGGGCCTG GAGAAAGACT GCAGGGGGCT GTTTTTGGAT 
TATGATAAGG ATGATGTGAC GGCACAACCG AGGGAATGGA AGAAATACGA GTTTCACTAT 
GACAATGTGC TTTGGGCTTT TCTCACTCTC TTCACTGTTT CTACGGGAGA GGGCTGGCCT 
ACGGTTTTGA AGCACTCCGT GGATGCCACT TTTGAGGATC AGGGTCCCAG TCCAGGCTAC 
CGCATTGAGA TGTCTATTTT TTACGTGGTC TACTTTGTGG TTTTCCCTTT CTTCTTCGTC 
AACATTTTTG TAGCCTTGAT AATCATCACA TTCCAGGAAC AGGGTGATAA GGTCTTGTCA 
GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC TGCATCGACT TTGCTATCAA TGCCAAACCT 
CTGACACGCT ACATGCCGCA GAACCAGCAG TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG 
GTCTCTCCGC CGTTTGAGTA CTCCATCATG ATCATGATTG CTCTCAACAC TGTGGTGCTC 
ATGATGAAGT TCCATGGGGC GCCTGACTTT TATGAGGCAA TGCTTAAGTA TCTCAACATT 
GTCTTCACTG TTCTCTTTTC ACTGGAGTGT ATTCTCAAGA TTATTGCTTT CGGCCCCTTG 
AATTACCTGA AAGATGCCTG GAATGTGTTT GACTTTGTGA CTGTGCTGGG CAGCATCACT 
GACATTTTAG TCACTGAGAT TAACACAACG GAGCGTCAGC TGAACTTCAG TTTCCTGAGG 
CTCTTTAGAG CAGCCCGACT CATCAAGCTC CTCCGGCAGG GATACACCAT TCGCATCCTG 
CTCTGGACAT TTGTCCAGTC TTTTAAGGCA CTGCCTTATG TTTGCCTTCT AATCGCCATG 
CTGTTTTTCA TCTACGCCAT TATTGGGATG CAGGTGTTTG GAAACATTGA TTTGAATGAT 
GACACGGCAA TCAACCGTCA CAACAACTTC CGCACTTTCC TCCAAGCTCT TATGCTTTTG 
TTCAGGAGTG CCACTGGCGA GGCTTGGCAT GATATCATGC TGTCGTGTCT GAGCGAGCGC 
ACCTGTGATC CGAGCTCAGG CACCCTTGGA AAGGAGTGTG GGAGTGACTT TGCCTACTTC 
TACTTTGTCT CCTTCATTTT CCTCTGCTCA TTTCTGATGT TGAACCTGTT TGTGGCCGTC 
ATTATGGACA ACTTTGAATA TCTGACACGC GACTCCTCCA TCCTGGGCCC TCACCACCTG 
GATGAGTTTA TTCGAGTGTG GGCGGAGTAT GACCCAGCCG CATGTGGCCG TATCAAGTAC 
CTCGATATGT ATGAGATGCT GCTCCACATG TCCCCACCTT TAGGTTTGGG AAAGAAATGT 
CCTCCACGAA TCGCCTACAA GCGTCTGGTG AAAATGAACA TGCCCATTGC GGATGACAAC 
AGTGTACACT TTACCTCAAC GCTGATGGCT CTGATCCGTA CTGCCCTGGA GATCAAACTG 
GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA GATGCTGAAC TGAAGAAGGA GCTCTCTACA 
GTTTGGCCAA ACCTGTCTCA GAAGACCATG GACCTGCTGG TCACTCCACA TAAACCTAAT 
GAGCTCACAG TGGGGAAGGT GTATGCAGCT CTTATGATCT TTGACTATTA CAAGCAGAAC 
AGAGCAAAAA GACTTCAGAT GCAGCAACAG CAGCTGAAAG AACAGGCAGG ACCTGGATCT 
CAGAATAAAG TCAGCGCTCT CTTAGAGCCC ATTCTCCCAC TAACCCACAT GCAAGATCAG 
CCAGTGAACG GTGTCGATTC AGCTTCAGAG TGTCAAGCTC AGACCAGACC CAGCTCCACC 
ACACTCAACA ATGGACGCAC AACTCTTGAC AACACAATAA AAGGGTCTTC ATCCTGGGCC 
TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT AAACGGAGAC CAATGTCTAG AGGTCAATCT 
GAGGATGCCT CAAATGCAAA CACTGCCCAG GAGTCAGTAG AAATGAGAAA GATGGAGAAC 
AGTGCCAACA CAGTGACCTC TGGTGGCCTT GAGGGCCAAG GACGTGCAGC GTCCATGCCA 
AAACTCAATG CTGAGATGCA GAGGAGTCAT TCCCGTCAAT CTCCTGGAAC ATTACTGGAA 
CCCATTCCAG ATTCCCCCAT GAGGCGCTCT GCATCCACTT TTGCACCACA GCGCCCTCAC 
GAGGTGAATC TGAATGACTA CATTCTGGAG AAACCTGTTC AGGAACGCCA TCACCACCAT 
CACCATCATC ACCGATGCCA TCATCGCCGG GACAGAGACA AGAAACAGAG ATCTCTGGAC 
AGGTCGCCTA CTGGCCATCA CAAAACTACA GGCACAGCCA CTGACTCTGT AGAGGAGAGG 
GCACATGACC GGGGTCGTTC CCATGAGAGG AAGCACCATT CATCTTCAGG AGAGAAGCAG 
CGGTATTACT CGTGTGATCG CTATGCCAGT CGGGAACACT GTTCCAGCAA ATCTGCCGTG 
GCAAGCTGTG CCACCTCACC CAGCGAGACC CAGGAGGCCA ATCTTAACAA GCAGGGCAGT 
GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG ACTTCAGGGG CGAGCACTCC AAGCCGCGGG 
CGCAGACAGC TGCCCCAGAC CCCCCTCACT CCTCGTCCCT CCGTGGCCTA CAGGACTGCC 
AACTCTTCCC CTGTGCAACT TCTGGCCACA ACGACCAGCT TGCTTCCCCC GAGTCCCGGC 
AGGCTGAGCC GTGGTCTGTC CGAGCACAAC GCTCTTTTAC TCAGCGGCTC TATCTCCAGC 
TCGCCGGCCC CCGTCAGTCG CATTAACTCA GAACCCTTCT TGGGGCCCCC GGACCACCCT 
GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC TTCCAAGAGA ATCCGAGCCC TCAGATGGGC 
CGTTCTCCTC AGACTGTAGG GATGCCCTTC GTGGCGATCC CCCCACCTCC CCCACAGCAG 
TTTCGCAGGG TCCCCAACGG GTACCACTTC TCCACGGGCC CCAGCGCCAG CAGAGGACGG 
GCACAGCAGT ACTACCCAGA GGCAGAGGTG GAGGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_021476176.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331851.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA.

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CloneID	ODa142642
Clone ID Related Accession (Same CDS sequence)	XM_021476176.1
Accession Version	XM_021476176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGAGG AACCAAGGGC AAAGACATCA GCACTATCAA GTCACTGAGG 
GTGTTGCGAG TCTTGCGACC TCTGAAGACC ATCAAACGTC TACCAAAACT GAAGGCCGTG 
TTTGATTGTG TGGTGAACTC CCTGAAGAAT GTCCTGAATA TCCTCATTGT CTACATTCTG 
TTCATGTTCA TTTTCGCTGT CATTGCTGTG CAGCTCTTCA AGGGCAAGTT TTTTTACTGC 
ACAGACGAGT CCAAGGGCCT GGAGAAAGAC TGCAGGGGGC TGTTTTTGGA TTATGATAAG 
GATGATGTGA CGGCACAACC GAGGGAATGG AAGAAATACG AGTTTCACTA TGACAATGTG 
CTTTGGGCTT TTCTCACTCT CTTCACTGTT TCTACGGGAG AGGGCTGGCC TACGGTTTTG 
AAGCACTCCG TGGATGCCAC TTTTGAGGAT CAGGGTCCCA GTCCAGGCTA CCGCATTGAG 
ATGTCTATTT TTTACGTGGT CTACTTTGTG GTTTTCCCTT TCTTCTTCGT CAACATTTTT 
GTAGCCTTGA TAATCATCAC ATTCCAGGAA CAGGGTGATA AGGTCTTGTC AGAGTGCAGT 
CTGGAGAAGA ATGAGAGAGC CTGCATCGAC TTTGCTATCA ATGCCAAACC TCTGACACGC 
TACATGCCGC AGAACCAGCA GTCCTTTCAG TACCGCCTGT GGAAGTTTGT GGTCTCTCCG 
CCGTTTGAGT ACTCCATCAT GATCATGATT GCTCTCAACA CTGTGGTGCT CATGATGAAG 
TTCCATGGGG CGCCTGACTT TTATGAGGCA ATGCTTAAGT ATCTCAACAT TGTCTTCACT 
GTTCTCTTTT CACTGGAGTG TATTCTCAAG ATTATTGCTT TCGGCCCCTT GAATTACCTG 
AAAGATGCCT GGAATGTGTT TGACTTTGTG ACTGTGCTGG GCAGCATCAC TGACATTTTA 
GTCACTGAGA TTAACACAAC GGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA 
GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA 
TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC 
ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA 
ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT 
GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT 
CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC 
TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC 
AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT 
ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG 
TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA 
ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC 
TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC 
ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA 
AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA 
GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA 
AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA 
GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC 
GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC 
AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA 
TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC 
TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC 
ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT 
GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA 
GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT 
CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT 
CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT 
ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC 
CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC 
TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT 
GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT 
AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG 
CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC 
CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC 
CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC 
CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG 
CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT 
CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG 
GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG 
TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331851.1
CDS	1..7116
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_021476176.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476176.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGAGG AACCAAGGGC AAAGACATCA GCACTATCAA GTCACTGAGG 
GTGTTGCGAG TCTTGCGACC TCTGAAGACC ATCAAACGTC TACCAAAACT GAAGGCCGTG 
TTTGATTGTG TGGTGAACTC CCTGAAGAAT GTCCTGAATA TCCTCATTGT CTACATTCTG 
TTCATGTTCA TTTTCGCTGT CATTGCTGTG CAGCTCTTCA AGGGCAAGTT TTTTTACTGC 
ACAGACGAGT CCAAGGGCCT GGAGAAAGAC TGCAGGGGGC TGTTTTTGGA TTATGATAAG 
GATGATGTGA CGGCACAACC GAGGGAATGG AAGAAATACG AGTTTCACTA TGACAATGTG 
CTTTGGGCTT TTCTCACTCT CTTCACTGTT TCTACGGGAG AGGGCTGGCC TACGGTTTTG 
AAGCACTCCG TGGATGCCAC TTTTGAGGAT CAGGGTCCCA GTCCAGGCTA CCGCATTGAG 
ATGTCTATTT TTTACGTGGT CTACTTTGTG GTTTTCCCTT TCTTCTTCGT CAACATTTTT 
GTAGCCTTGA TAATCATCAC ATTCCAGGAA CAGGGTGATA AGGTCTTGTC AGAGTGCAGT 
CTGGAGAAGA ATGAGAGAGC CTGCATCGAC TTTGCTATCA ATGCCAAACC TCTGACACGC 
TACATGCCGC AGAACCAGCA GTCCTTTCAG TACCGCCTGT GGAAGTTTGT GGTCTCTCCG 
CCGTTTGAGT ACTCCATCAT GATCATGATT GCTCTCAACA CTGTGGTGCT CATGATGAAG 
TTCCATGGGG CGCCTGACTT TTATGAGGCA ATGCTTAAGT ATCTCAACAT TGTCTTCACT 
GTTCTCTTTT CACTGGAGTG TATTCTCAAG ATTATTGCTT TCGGCCCCTT GAATTACCTG 
AAAGATGCCT GGAATGTGTT TGACTTTGTG ACTGTGCTGG GCAGCATCAC TGACATTTTA 
GTCACTGAGA TTAACACAAC GGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA 
GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA 
TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC 
ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA 
ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT 
GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT 
CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC 
TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC 
AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT 
ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG 
TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA 
ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC 
TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC 
ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA 
AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA 
GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA 
AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA 
GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC 
GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC 
AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA 
TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC 
TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC 
ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT 
GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA 
GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT 
CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT 
CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT 
ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC 
CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC 
TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT 
GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT 
AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG 
CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC 
CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC 
CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC 
CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG 
CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT 
CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG 
GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG 
TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_021476178.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331853.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA.

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CloneID	ODa142643
Clone ID Related Accession (Same CDS sequence)	XM_021476178.1
Accession Version	XM_021476178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACGAGCGTC AGCTGAACTT CAGTTTCCTG AGGCTCTTTA GAGCAGCCCG ACTCATCAAG 
CTCCTCCGGC AGGGATACAC CATTCGCATC CTGCTCTGGA CATTTGTCCA GTCTTTTAAG 
GCACTGCCTT ATGTTTGCCT TCTAATCGCC ATGCTGTTTT TCATCTACGC CATTATTGGG 
ATGCAGGTGT TTGGAAACAT TGATTTGAAT GATGACACGG CAATCAACCG TCACAACAAC 
TTCCGCACTT TCCTCCAAGC TCTTATGCTT TTGTTCAGGA GTGCCACTGG CGAGGCTTGG 
CATGATATCA TGCTGTCGTG TCTGAGCGAG CGCACCTGTG ATCCGAGCTC AGGCACCCTT 
GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT TTTCCTCTGC 
TCATTTCTGA TGTTGAACCT GTTTGTGGCC GTCATTATGG ACAACTTTGA ATATCTGACA 
CGCGACTCCT CCATCCTGGG CCCTCACCAC CTGGATGAGT TTATTCGAGT GTGGGCGGAG 
TATGACCCAG CCGCATGTGG CCGTATCAAG TACCTCGATA TGTATGAGAT GCTGCTCCAC 
ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA TGTCCTCCAC GAATCGCCTA CAAGCGTCTG 
GTGAAAATGA ACATGCCCAT TGCGGATGAC AACAGTGTAC ACTTTACCTC AACGCTGATG 
GCTCTGATCC GTACTGCCCT GGAGATCAAA CTGGCTTCAG GCATGGTGGC TCAGCGTCTG 
TCAGATGCTG AACTGAAGAA GGAGCTCTCT ACAGTTTGGC CAAACCTGTC TCAGAAGACC 
ATGGACCTGC TGGTCACTCC ACATAAACCT AATGAGCTCA CAGTGGGGAA GGTGTATGCA 
GCTCTTATGA TCTTTGACTA TTACAAGCAG AACAGAGCAA AAAGACTTCA GATGCAGCAA 
CAGCAGCTGA AAGAACAGGC AGGACCTGGA TCTCAGAATA AAGTCAGCGC TCTCTTAGAG 
CCCATTCTCC CACTAACCCA CATGCAAGAT CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA 
GAGTGTCAAG CTCAGACCAG ACCCAGCTCC ACCACACTCA ACAATGGACG CACAACTCTT 
GACAACACAA TAAAAGGGTC TTCATCCTGG GCCTCAGAGA AATCCAAGGA AGTCCAAAAG 
TCTAAACGGA GACCAATGTC TAGAGGTCAA TCTGAGGATG CCTCAAATGC AAACACTGCC 
CAGGAGTCAG TAGAAATGAG AAAGATGGAG AACAGTGCCA ACACAGTGAC CTCTGGTGGC 
CTTGAGGGCC AAGGACGTGC AGCGTCCATG CCAAAACTCA ATGCTGAGAT GCAGAGGAGT 
CATTCCCGTC AATCTCCTGG AACATTACTG GAACCCATTC CAGATTCCCC CATGAGGCGC 
TCTGCATCCA CTTTTGCACC ACAGCGCCCT CACGAGGTGA ATCTGAATGA CTACATTCTG 
GAGAAACCTG TTCAGGAACG CCATCACCAC CATCACCATC ATCACCGATG CCATCATCGC 
CGGGACAGAG ACAAGAAACA GAGATCTCTG GACAGGTCGC CTACTGGCCA TCACAAAACT 
ACAGGCACAG CCACTGACTC TGTAGAGGAG AGGGCACATG ACCGGGGTCG TTCCCATGAG 
AGGAAGCACC ATTCATCTTC AGGAGAGAAG CAGCGGTATT ACTCGTGTGA TCGCTATGCC 
AGTCGGGAAC ACTGTTCCAG CAAATCTGCC GTGGCAAGCT GTGCCACCTC ACCCAGCGAG 
ACCCAGGAGG CCAATCTTAA CAAGCAGGGC AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG 
TTGACTTCAG GGGCGAGCAC TCCAAGCCGC GGGCGCAGAC AGCTGCCCCA GACCCCCCTC 
ACTCCTCGTC CCTCCGTGGC CTACAGGACT GCCAACTCTT CCCCTGTGCA ACTTCTGGCC 
ACAACGACCA GCTTGCTTCC CCCGAGTCCC GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC 
AACGCTCTTT TACTCAGCGG CTCTATCTCC AGCTCGCCGG CCCCCGTCAG TCGCATTAAC 
TCAGAACCCT TCTTGGGGCC CCCGGACCAC CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT 
GTCTTCCAAG AGAATCCGAG CCCTCAGATG GGCCGTTCTC CTCAGACTGT AGGGATGCCC 
TTCGTGGCGA TCCCCCCACC TCCCCCACAG CAGTTTCGCA GGGTCCCCAA CGGGTACCAC 
TTCTCCACGG GCCCCAGCGC CAGCAGAGGA CGGGCACAGC AGTACTACCC AGAGGCAGAG 
GTGGAGGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331853.1
CDS	1..7098
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_021476178.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476178.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACGAGCGTC AGCTGAACTT CAGTTTCCTG AGGCTCTTTA GAGCAGCCCG ACTCATCAAG 
CTCCTCCGGC AGGGATACAC CATTCGCATC CTGCTCTGGA CATTTGTCCA GTCTTTTAAG 
GCACTGCCTT ATGTTTGCCT TCTAATCGCC ATGCTGTTTT TCATCTACGC CATTATTGGG 
ATGCAGGTGT TTGGAAACAT TGATTTGAAT GATGACACGG CAATCAACCG TCACAACAAC 
TTCCGCACTT TCCTCCAAGC TCTTATGCTT TTGTTCAGGA GTGCCACTGG CGAGGCTTGG 
CATGATATCA TGCTGTCGTG TCTGAGCGAG CGCACCTGTG ATCCGAGCTC AGGCACCCTT 
GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT TTTCCTCTGC 
TCATTTCTGA TGTTGAACCT GTTTGTGGCC GTCATTATGG ACAACTTTGA ATATCTGACA 
CGCGACTCCT CCATCCTGGG CCCTCACCAC CTGGATGAGT TTATTCGAGT GTGGGCGGAG 
TATGACCCAG CCGCATGTGG CCGTATCAAG TACCTCGATA TGTATGAGAT GCTGCTCCAC 
ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA TGTCCTCCAC GAATCGCCTA CAAGCGTCTG 
GTGAAAATGA ACATGCCCAT TGCGGATGAC AACAGTGTAC ACTTTACCTC AACGCTGATG 
GCTCTGATCC GTACTGCCCT GGAGATCAAA CTGGCTTCAG GCATGGTGGC TCAGCGTCTG 
TCAGATGCTG AACTGAAGAA GGAGCTCTCT ACAGTTTGGC CAAACCTGTC TCAGAAGACC 
ATGGACCTGC TGGTCACTCC ACATAAACCT AATGAGCTCA CAGTGGGGAA GGTGTATGCA 
GCTCTTATGA TCTTTGACTA TTACAAGCAG AACAGAGCAA AAAGACTTCA GATGCAGCAA 
CAGCAGCTGA AAGAACAGGC AGGACCTGGA TCTCAGAATA AAGTCAGCGC TCTCTTAGAG 
CCCATTCTCC CACTAACCCA CATGCAAGAT CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA 
GAGTGTCAAG CTCAGACCAG ACCCAGCTCC ACCACACTCA ACAATGGACG CACAACTCTT 
GACAACACAA TAAAAGGGTC TTCATCCTGG GCCTCAGAGA AATCCAAGGA AGTCCAAAAG 
TCTAAACGGA GACCAATGTC TAGAGGTCAA TCTGAGGATG CCTCAAATGC AAACACTGCC 
CAGGAGTCAG TAGAAATGAG AAAGATGGAG AACAGTGCCA ACACAGTGAC CTCTGGTGGC 
CTTGAGGGCC AAGGACGTGC AGCGTCCATG CCAAAACTCA ATGCTGAGAT GCAGAGGAGT 
CATTCCCGTC AATCTCCTGG AACATTACTG GAACCCATTC CAGATTCCCC CATGAGGCGC 
TCTGCATCCA CTTTTGCACC ACAGCGCCCT CACGAGGTGA ATCTGAATGA CTACATTCTG 
GAGAAACCTG TTCAGGAACG CCATCACCAC CATCACCATC ATCACCGATG CCATCATCGC 
CGGGACAGAG ACAAGAAACA GAGATCTCTG GACAGGTCGC CTACTGGCCA TCACAAAACT 
ACAGGCACAG CCACTGACTC TGTAGAGGAG AGGGCACATG ACCGGGGTCG TTCCCATGAG 
AGGAAGCACC ATTCATCTTC AGGAGAGAAG CAGCGGTATT ACTCGTGTGA TCGCTATGCC 
AGTCGGGAAC ACTGTTCCAG CAAATCTGCC GTGGCAAGCT GTGCCACCTC ACCCAGCGAG 
ACCCAGGAGG CCAATCTTAA CAAGCAGGGC AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG 
TTGACTTCAG GGGCGAGCAC TCCAAGCCGC GGGCGCAGAC AGCTGCCCCA GACCCCCCTC 
ACTCCTCGTC CCTCCGTGGC CTACAGGACT GCCAACTCTT CCCCTGTGCA ACTTCTGGCC 
ACAACGACCA GCTTGCTTCC CCCGAGTCCC GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC 
AACGCTCTTT TACTCAGCGG CTCTATCTCC AGCTCGCCGG CCCCCGTCAG TCGCATTAAC 
TCAGAACCCT TCTTGGGGCC CCCGGACCAC CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT 
GTCTTCCAAG AGAATCCGAG CCCTCAGATG GGCCGTTCTC CTCAGACTGT AGGGATGCCC 
TTCGTGGCGA TCCCCCCACC TCCCCCACAG CAGTTTCGCA GGGTCCCCAA CGGGTACCAC 
TTCTCCACGG GCCCCAGCGC CAGCAGAGGA CGGGCACAGC AGTACTACCC AGAGGCAGAG 
GTGGAGGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_021476179.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331854.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA.

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CloneID	ODa142644
Clone ID Related Accession (Same CDS sequence)	XM_021476179.1
Accession Version	XM_021476179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACGAGCGTC AGCTGAACTT CAGTTTCCTG AGGCTCTTTA GAGCAGCCCG ACTCATCAAG 
CTCCTCCGGC AGGGATACAC CATTCGCATC CTGCTCTGGA CATTTGTCCA GTCTTTTAAG 
GCACTGCCTT ATGTTTGCCT TCTAATCGCC ATGCTGTTTT TCATCTACGC CATTATTGGG 
ATGCAGGTGT TTGGAAACAT TGATTTGAAT GATGACACGG CAATCAACCG TCACAACAAC 
TTCCGCACTT TCCTCCAAGC TCTTATGCTT TTGTTCAGGA GTGCCACTGG CGAGGCTTGG 
CATGATATCA TGCTGTCGTG TCTGAGCGAG CGCACCTGTG ATCCGAGCTC AGGCACCCTT 
GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT TTTCCTCTGC 
TCATTTCTGA TGTTGAACCT GTTTGTGGCC GTCATTATGG ACAACTTTGA ATATCTGACA 
CGCGACTCCT CCATCCTGGG CCCTCACCAC CTGGATGAGT TTATTCGAGT GTGGGCGGAG 
TATGACCCAG CCGCATGCGG ACGGATTTCC TATAAGGATA TGTACAATTT GTTGCGAATT 
ATCTCGCCCC CCCTTGGCTT AGGGAAGAAC TGCCCTAATA GGGTAGCATA CAAGCGTCTG 
GTGAAAATGA ACATGCCCAT TGCGGATGAC AACAGTGTAC ACTTTACCTC AACGCTGATG 
GCTCTGATCC GTACTGCCCT GGAGATCAAA CTGGCTTCAG GCATGGTGGC TCAGCGTCTG 
TCAGATGCTG AACTGAAGAA GGAGCTCTCT ACAGTTTGGC CAAACCTGTC TCAGAAGACC 
ATGGACCTGC TGGTCACTCC ACATAAACCT AATGAGCTCA CAGTGGGGAA GGTGTATGCA 
GCTCTTATGA TCTTTGACTA TTACAAGCAG AACAGAGCAA AAAGACTTCA GATGCAGCAA 
CAGCAGCTGA AAGAACAGGC AGGACCTGGA TCTCAGAATA AAGTCAGCGC TCTCTTAGAG 
CCCATTCTCC CACTAACCCA CATGCAAGAT CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA 
GAGTGTCAAG CTCAGACCAG ACCCAGCTCC ACCACACTCA ACAATGGACG CACAACTCTT 
GACAACACAA TAAAAGGGTC TTCATCCTGG GCCTCAGAGA AATCCAAGGA AGTCCAAAAG 
TCTAAACGGA GACCAATGTC TAGAGGTCAA TCTGAGGATG CCTCAAATGC AAACACTGCC 
CAGGAGTCAG TAGAAATGAG AAAGATGGAG AACAGTGCCA ACACAGTGAC CTCTGGTGGC 
CTTGAGGGCC AAGGACGTGC AGCGTCCATG CCAAAACTCA ATGCTGAGAT GCAGAGGAGT 
CATTCCCGTC AATCTCCTGG AACATTACTG GAACCCATTC CAGATTCCCC CATGAGGCGC 
TCTGCATCCA CTTTTGCACC ACAGCGCCCT CACGAGGTGA ATCTGAATGA CTACATTCTG 
GAGAAACCTG TTCAGGAACG CCATCACCAC CATCACCATC ATCACCGATG CCATCATCGC 
CGGGACAGAG ACAAGAAACA GAGATCTCTG GACAGGTCGC CTACTGGCCA TCACAAAACT 
ACAGGCACAG CCACTGACTC TGTAGAGGAG AGGGCACATG ACCGGGGTCG TTCCCATGAG 
AGGAAGCACC ATTCATCTTC AGGAGAGAAG CAGCGGTATT ACTCGTGTGA TCGCTATGCC 
AGTCGGGAAC ACTGTTCCAG CAAATCTGCC GTGGCAAGCT GTGCCACCTC ACCCAGCGAG 
ACCCAGGAGG CCAATCTTAA CAAGCAGGGC AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG 
TTGACTTCAG GGGCGAGCAC TCCAAGCCGC GGGCGCAGAC AGCTGCCCCA GACCCCCCTC 
ACTCCTCGTC CCTCCGTGGC CTACAGGACT GCCAACTCTT CCCCTGTGCA ACTTCTGGCC 
ACAACGACCA GCTTGCTTCC CCCGAGTCCC GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC 
AACGCTCTTT TACTCAGCGG CTCTATCTCC AGCTCGCCGG CCCCCGTCAG TCGCATTAAC 
TCAGAACCCT TCTTGGGGCC CCCGGACCAC CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT 
GTCTTCCAAG AGAATCCGAG CCCTCAGATG GGCCGTTCTC CTCAGACTGT AGGGATGCCC 
TTCGTGGCGA TCCCCCCACC TCCCCCACAG CAGTTTCGCA GGGTCCCCAA CGGGTACCAC 
TTCTCCACGG GCCCCAGCGC CAGCAGAGGA CGGGCACAGC AGTACTACCC AGAGGCAGAG 
GTGGAGGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331854.1
CDS	1..7098
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYKDMYNLLRIISPPLGLGKNCPNRVAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_021476179.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476179.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACGAGCGTC AGCTGAACTT CAGTTTCCTG AGGCTCTTTA GAGCAGCCCG ACTCATCAAG 
CTCCTCCGGC AGGGATACAC CATTCGCATC CTGCTCTGGA CATTTGTCCA GTCTTTTAAG 
GCACTGCCTT ATGTTTGCCT TCTAATCGCC ATGCTGTTTT TCATCTACGC CATTATTGGG 
ATGCAGGTGT TTGGAAACAT TGATTTGAAT GATGACACGG CAATCAACCG TCACAACAAC 
TTCCGCACTT TCCTCCAAGC TCTTATGCTT TTGTTCAGGA GTGCCACTGG CGAGGCTTGG 
CATGATATCA TGCTGTCGTG TCTGAGCGAG CGCACCTGTG ATCCGAGCTC AGGCACCCTT 
GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT TTTCCTCTGC 
TCATTTCTGA TGTTGAACCT GTTTGTGGCC GTCATTATGG ACAACTTTGA ATATCTGACA 
CGCGACTCCT CCATCCTGGG CCCTCACCAC CTGGATGAGT TTATTCGAGT GTGGGCGGAG 
TATGACCCAG CCGCATGCGG ACGGATTTCC TATAAGGATA TGTACAATTT GTTGCGAATT 
ATCTCGCCCC CCCTTGGCTT AGGGAAGAAC TGCCCTAATA GGGTAGCATA CAAGCGTCTG 
GTGAAAATGA ACATGCCCAT TGCGGATGAC AACAGTGTAC ACTTTACCTC AACGCTGATG 
GCTCTGATCC GTACTGCCCT GGAGATCAAA CTGGCTTCAG GCATGGTGGC TCAGCGTCTG 
TCAGATGCTG AACTGAAGAA GGAGCTCTCT ACAGTTTGGC CAAACCTGTC TCAGAAGACC 
ATGGACCTGC TGGTCACTCC ACATAAACCT AATGAGCTCA CAGTGGGGAA GGTGTATGCA 
GCTCTTATGA TCTTTGACTA TTACAAGCAG AACAGAGCAA AAAGACTTCA GATGCAGCAA 
CAGCAGCTGA AAGAACAGGC AGGACCTGGA TCTCAGAATA AAGTCAGCGC TCTCTTAGAG 
CCCATTCTCC CACTAACCCA CATGCAAGAT CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA 
GAGTGTCAAG CTCAGACCAG ACCCAGCTCC ACCACACTCA ACAATGGACG CACAACTCTT 
GACAACACAA TAAAAGGGTC TTCATCCTGG GCCTCAGAGA AATCCAAGGA AGTCCAAAAG 
TCTAAACGGA GACCAATGTC TAGAGGTCAA TCTGAGGATG CCTCAAATGC AAACACTGCC 
CAGGAGTCAG TAGAAATGAG AAAGATGGAG AACAGTGCCA ACACAGTGAC CTCTGGTGGC 
CTTGAGGGCC AAGGACGTGC AGCGTCCATG CCAAAACTCA ATGCTGAGAT GCAGAGGAGT 
CATTCCCGTC AATCTCCTGG AACATTACTG GAACCCATTC CAGATTCCCC CATGAGGCGC 
TCTGCATCCA CTTTTGCACC ACAGCGCCCT CACGAGGTGA ATCTGAATGA CTACATTCTG 
GAGAAACCTG TTCAGGAACG CCATCACCAC CATCACCATC ATCACCGATG CCATCATCGC 
CGGGACAGAG ACAAGAAACA GAGATCTCTG GACAGGTCGC CTACTGGCCA TCACAAAACT 
ACAGGCACAG CCACTGACTC TGTAGAGGAG AGGGCACATG ACCGGGGTCG TTCCCATGAG 
AGGAAGCACC ATTCATCTTC AGGAGAGAAG CAGCGGTATT ACTCGTGTGA TCGCTATGCC 
AGTCGGGAAC ACTGTTCCAG CAAATCTGCC GTGGCAAGCT GTGCCACCTC ACCCAGCGAG 
ACCCAGGAGG CCAATCTTAA CAAGCAGGGC AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG 
TTGACTTCAG GGGCGAGCAC TCCAAGCCGC GGGCGCAGAC AGCTGCCCCA GACCCCCCTC 
ACTCCTCGTC CCTCCGTGGC CTACAGGACT GCCAACTCTT CCCCTGTGCA ACTTCTGGCC 
ACAACGACCA GCTTGCTTCC CCCGAGTCCC GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC 
AACGCTCTTT TACTCAGCGG CTCTATCTCC AGCTCGCCGG CCCCCGTCAG TCGCATTAAC 
TCAGAACCCT TCTTGGGGCC CCCGGACCAC CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT 
GTCTTCCAAG AGAATCCGAG CCCTCAGATG GGCCGTTCTC CTCAGACTGT AGGGATGCCC 
TTCGTGGCGA TCCCCCCACC TCCCCCACAG CAGTTTCGCA GGGTCCCCAA CGGGTACCAC 
TTCTCCACGG GCCCCAGCGC CAGCAGAGGA CGGGCACAGC AGTACTACCC AGAGGCAGAG 
GTGGAGGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_021476180.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331855.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA.

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CloneID	ODa142645
Clone ID Related Accession (Same CDS sequence)	XM_021476180.1
Accession Version	XM_021476180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC 
AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC 
ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT 
CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT 
GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT 
CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT 
CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC 
TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG 
TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC 
CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC 
CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG 
GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT 
GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG 
GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG 
GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA 
CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT 
TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA 
GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC 
ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA 
CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT 
TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT 
AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA 
AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA 
GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA 
ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA 
CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC 
CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG 
AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT 
GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA 
GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC 
AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC 
AAGCAGTGGG TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331855.1
CDS	1..6618
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQWVG

Target ORF information:

RefSeq Version	XM_021476180.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476180.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC 
AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC 
ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT 
CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT 
GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT 
CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT 
CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC 
TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG 
TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC 
CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC 
CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG 
GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT 
GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG 
GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG 
GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA 
CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT 
TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA 
GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC 
ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA 
CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT 
TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT 
AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA 
AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA 
GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA 
ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA 
CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC 
CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG 
AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT 
GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA 
GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC 
AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC 
AAGCAGTGGG TGGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_021476181.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331856.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA.

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CloneID	ODa142646
Clone ID Related Accession (Same CDS sequence)	XM_021476181.1
Accession Version	XM_021476181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG 
CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG 
AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC 
GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG 
GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA 
CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC 
ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT 
GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC 
GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC 
ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG 
AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC 
CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC 
ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT 
GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG 
GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT 
GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC 
ACAACGGAGC GTCAGCTGAA CTTCAGTTTC CTGAGGCTCT TTAGAGCAGC CCGACTCATC 
AAGCTCCTCC GGCAGGGATA CACCATTCGC ATCCTGCTCT GGACATTTGT CCAGTCTTTT 
AAGGCACTGC CTTATGTTTG CCTTCTAATC GCCATGCTGT TTTTCATCTA CGCCATTATT 
GGGATGCAGG TGTTTGGAAA CATTGATTTG AATGATGACA CGGCAATCAA CCGTCACAAC 
AACTTCCGCA CTTTCCTCCA AGCTCTTATG CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT 
TGGCATGATA TCATGCTGTC GTGTCTGAGC GAGCGCACCT GTGATCCGAG CTCAGGCACC 
CTTGGAAAGG AGTGTGGGAG TGACTTTGCC TACTTCTACT TTGTCTCCTT CATTTTCCTC 
TGCTCATTTC TGATGTTGAA CCTGTTTGTG GCCGTCATTA TGGACAACTT TGAATATCTG 
ACACGCGACT CCTCCATCCT GGGCCCTCAC CACCTGGATG AGTTTATTCG AGTGTGGGCG 
GAGTATGACC CAGCCGCATG CGGACGGATT TCCTATAAGG ATATGTACAA TTTGTTGCGA 
ATTATCTCGC CCCCCCTTGG CTTAGGGAAG AACTGCCCTA ATAGGGTAGC ATACAAGCGT 
CTGGTGAAAA TGAACATGCC CATTGCGGAT GACAACAGTG TACACTTTAC CTCAACGCTG 
ATGGCTCTGA TCCGTACTGC CCTGGAGATC AAACTGGCTT CAGGCATGGT GGCTCAGCGT 
CTGTCAGATG CTGAACTGAA GAAGGAGCTC TCTACAGTTT GGCCAAACCT GTCTCAGAAG 
ACCATGGACC TGCTGGTCAC TCCACATAAA CCTAATGAGC TCACAGTGGG GAAGGTGTAT 
GCAGCTCTTA TGATCTTTGA CTATTACAAG CAGAACAGAG CAAAAAGACT TCAGATGCAG 
CAACAGCAGC TGAAAGAACA GGCAGGACCT GGATCTCAGA ATAAAGTCAG CGCTCTCTTA 
GAGCCCATTC TCCCACTAAC CCACATGCAA GATCAGCCAG TGAACGGTGT CGATTCAGCT 
TCAGAGTGTC AAGCTCAGAC CAGACCCAGC TCCACCACAC TCAACAATGG ACGCACAACT 
CTTGACAACA CAATAAAAGG GTCTTCATCC TGGGCCTCAG AGAAATCCAA GGAAGTCCAA 
AAGTCTAAAC GGAGACCAAT GTCTAGAGGT CAATCTGAGG ATGCCTCAAA TGCAAACACT 
GCCCAGGAGT CAGTAGAAAT GAGAAAGATG GAGAACAGTG CCAACACAGT GACCTCTGGT 
GGCCTTGAGG GCCAAGGACG TGCAGCGTCC ATGCCAAAAC TCAATGCTGA GATGCAGAGG 
AGTCATTCCC GTCAATCTCC TGGAACATTA CTGGAACCCA TTCCAGATTC CCCCATGAGG 
CGCTCTGCAT CCACTTTTGC ACCACAGCGC CCTCACGAGG TGAATCTGAA TGACTACATT 
CTGGAGAAAC CTGTTCAGGA ACGCCATCAC CACCATCACC ATCATCACCG ATGCCATCAT 
CGCCGGGACA GAGACAAGAA ACAGAGATCT CTGGACAGGT CGCCTACTGG CCATCACAAA 
ACTACAGGCA CAGCCACTGA CTCTGTAGAG GAGAGGGCAC ATGACCGGGG TCGTTCCCAT 
GAGAGGAAGC ACCATTCATC TTCAGGAGAG AAGCAGCGGT ATTACTCGTG TGATCGCTAT 
GCCAGTCGGG AACACTGTTC CAGCAAATCT GCCGTGGCAA GCTGTGCCAC CTCACCCAGC 
GAGACCCAGG AGGCCAATCT TAACAAGCAG GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG 
TTGTTGACTT CAGGGGCGAG CACTCCAAGC CGCGGGCGCA GACAGCTGCC CCAGACCCCC 
CTCACTCCTC GTCCCTCCGT GGCCTACAGG ACTGCCAACT CTTCCCCTGT GCAACTTCTG 
GCCACAACGA CCAGCTTGCT TCCCCCGAGT CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG 
CACAACGCTC TTTTACTCAG CGGCTCTATC TCCAGCTCGC CGGCCCCCGT CAGTCGCATT 
AACTCAGAAC CCTTCTTGGG GCCCCCGGAC CACCCTGCTG GCTTGCAGCC CGAAGCCTTC 
GCTGTCTTCC AAGAGAATCC GAGCCCTCAG ATGGGCCGTT CTCCTCAGAC TGTAGGGATG 
CCCTTCGTGG CGATCCCCCC ACCTCCCCCA CAGCAGTTTC GCAGGGTCCC CAACGGGTAC 
CACTTCTCCA CGGGCCCCAG CGCCAGCAGA GGACGGGCAC AGCAGTACTA CCCAGAGGCA 
GAGGTGGAGG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331856.1
CDS	637..7677
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYKDMYNLLRIISPPLGLGKNCPNRVAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_021476181.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476181.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG 
CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG 
AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC 
GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG 
GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA 
CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC 
ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT 
GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC 
GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC 
ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG 
AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC 
CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC 
ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT 
GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG 
GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT 
GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC 
ACAACGGAGC GTCAGCTGAA CTTCAGTTTC CTGAGGCTCT TTAGAGCAGC CCGACTCATC 
AAGCTCCTCC GGCAGGGATA CACCATTCGC ATCCTGCTCT GGACATTTGT CCAGTCTTTT 
AAGGCACTGC CTTATGTTTG CCTTCTAATC GCCATGCTGT TTTTCATCTA CGCCATTATT 
GGGATGCAGG TGTTTGGAAA CATTGATTTG AATGATGACA CGGCAATCAA CCGTCACAAC 
AACTTCCGCA CTTTCCTCCA AGCTCTTATG CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT 
TGGCATGATA TCATGCTGTC GTGTCTGAGC GAGCGCACCT GTGATCCGAG CTCAGGCACC 
CTTGGAAAGG AGTGTGGGAG TGACTTTGCC TACTTCTACT TTGTCTCCTT CATTTTCCTC 
TGCTCATTTC TGATGTTGAA CCTGTTTGTG GCCGTCATTA TGGACAACTT TGAATATCTG 
ACACGCGACT CCTCCATCCT GGGCCCTCAC CACCTGGATG AGTTTATTCG AGTGTGGGCG 
GAGTATGACC CAGCCGCATG CGGACGGATT TCCTATAAGG ATATGTACAA TTTGTTGCGA 
ATTATCTCGC CCCCCCTTGG CTTAGGGAAG AACTGCCCTA ATAGGGTAGC ATACAAGCGT 
CTGGTGAAAA TGAACATGCC CATTGCGGAT GACAACAGTG TACACTTTAC CTCAACGCTG 
ATGGCTCTGA TCCGTACTGC CCTGGAGATC AAACTGGCTT CAGGCATGGT GGCTCAGCGT 
CTGTCAGATG CTGAACTGAA GAAGGAGCTC TCTACAGTTT GGCCAAACCT GTCTCAGAAG 
ACCATGGACC TGCTGGTCAC TCCACATAAA CCTAATGAGC TCACAGTGGG GAAGGTGTAT 
GCAGCTCTTA TGATCTTTGA CTATTACAAG CAGAACAGAG CAAAAAGACT TCAGATGCAG 
CAACAGCAGC TGAAAGAACA GGCAGGACCT GGATCTCAGA ATAAAGTCAG CGCTCTCTTA 
GAGCCCATTC TCCCACTAAC CCACATGCAA GATCAGCCAG TGAACGGTGT CGATTCAGCT 
TCAGAGTGTC AAGCTCAGAC CAGACCCAGC TCCACCACAC TCAACAATGG ACGCACAACT 
CTTGACAACA CAATAAAAGG GTCTTCATCC TGGGCCTCAG AGAAATCCAA GGAAGTCCAA 
AAGTCTAAAC GGAGACCAAT GTCTAGAGGT CAATCTGAGG ATGCCTCAAA TGCAAACACT 
GCCCAGGAGT CAGTAGAAAT GAGAAAGATG GAGAACAGTG CCAACACAGT GACCTCTGGT 
GGCCTTGAGG GCCAAGGACG TGCAGCGTCC ATGCCAAAAC TCAATGCTGA GATGCAGAGG 
AGTCATTCCC GTCAATCTCC TGGAACATTA CTGGAACCCA TTCCAGATTC CCCCATGAGG 
CGCTCTGCAT CCACTTTTGC ACCACAGCGC CCTCACGAGG TGAATCTGAA TGACTACATT 
CTGGAGAAAC CTGTTCAGGA ACGCCATCAC CACCATCACC ATCATCACCG ATGCCATCAT 
CGCCGGGACA GAGACAAGAA ACAGAGATCT CTGGACAGGT CGCCTACTGG CCATCACAAA 
ACTACAGGCA CAGCCACTGA CTCTGTAGAG GAGAGGGCAC ATGACCGGGG TCGTTCCCAT 
GAGAGGAAGC ACCATTCATC TTCAGGAGAG AAGCAGCGGT ATTACTCGTG TGATCGCTAT 
GCCAGTCGGG AACACTGTTC CAGCAAATCT GCCGTGGCAA GCTGTGCCAC CTCACCCAGC 
GAGACCCAGG AGGCCAATCT TAACAAGCAG GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG 
TTGTTGACTT CAGGGGCGAG CACTCCAAGC CGCGGGCGCA GACAGCTGCC CCAGACCCCC 
CTCACTCCTC GTCCCTCCGT GGCCTACAGG ACTGCCAACT CTTCCCCTGT GCAACTTCTG 
GCCACAACGA CCAGCTTGCT TCCCCCGAGT CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG 
CACAACGCTC TTTTACTCAG CGGCTCTATC TCCAGCTCGC CGGCCCCCGT CAGTCGCATT 
AACTCAGAAC CCTTCTTGGG GCCCCCGGAC CACCCTGCTG GCTTGCAGCC CGAAGCCTTC 
GCTGTCTTCC AAGAGAATCC GAGCCCTCAG ATGGGCCGTT CTCCTCAGAC TGTAGGGATG 
CCCTTCGTGG CGATCCCCCC ACCTCCCCCA CAGCAGTTTC GCAGGGTCCC CAACGGGTAC 
CACTTCTCCA CGGGCCCCAG CGCCAGCAGA GGACGGGCAC AGCAGTACTA CCCAGAGGCA 
GAGGTGGAGG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_021476182.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331857.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X17, mRNA.

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CloneID	ODa142647
Clone ID Related Accession (Same CDS sequence)	XM_021476182.1
Accession Version	XM_021476182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG 
CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG 
AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC 
GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG 
GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA 
CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC 
ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT 
GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC 
GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC 
ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG 
AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC 
CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC 
ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT 
GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG 
GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT 
GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC 
GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC 
CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA 
CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG 
CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC 
CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT 
GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA 
AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA 
TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC 
GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT 
GACCCAGCCG CATGCGGACG GATTTCCTAT AAGGATATGT ACAATTTGTT GCGAATTATC 
TCGCCCCCCC TTGGCTTAGG GAAGAACTGC CCTAATAGGG TAGCATACAA GCGTCTGGTG 
AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT 
CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA 
GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG 
GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT 
CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG 
CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC 
ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG 
TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC 
AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT 
AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG 
GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT 
GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT 
TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT 
GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG 
AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG 
GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA 
GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG 
AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT 
CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC 
CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG 
ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT 
CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA 
ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC 
GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA 
GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC 
TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC 
GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC 
TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG 
GAGGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331857.1
CDS	637..7671
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYKDMYNLLRIISPPLGLGKNCPNRVAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_021476182.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476182.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG 
CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG 
AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC 
GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG 
GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA 
CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC 
ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT 
GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC 
GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC 
ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG 
AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC 
CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC 
ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT 
GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG 
GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT 
GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC 
GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC 
CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA 
CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG 
CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC 
CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT 
GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA 
AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA 
TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC 
GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT 
GACCCAGCCG CATGCGGACG GATTTCCTAT AAGGATATGT ACAATTTGTT GCGAATTATC 
TCGCCCCCCC TTGGCTTAGG GAAGAACTGC CCTAATAGGG TAGCATACAA GCGTCTGGTG 
AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT 
CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA 
GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG 
GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT 
CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG 
CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC 
ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG 
TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC 
AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT 
AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG 
GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT 
GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT 
TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT 
GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG 
AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG 
GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA 
GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG 
AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT 
CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC 
CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG 
ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT 
CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA 
ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC 
GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA 
GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC 
TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC 
GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC 
TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG 
GAGGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_017356095.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211584.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA.

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CloneID	ODa59085
Clone ID Related Accession (Same CDS sequence)	XM_017356095.1 , XM_017356095.2
Accession Version	XM_017356095.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CGAGTTATGT CTGCTCCCCT 
GCACATCACT ACTTGAGTAA AGAACAGCAG CGTTCTCTAA AAATGATGTC CGTGTGGGAG 
CAGCGCACCA CTCAGATCCG AAAGCACATG CTAGCCAGCA GTGAGGCGTT GTACCAAGAA 
GAGCTCAATC CACGGCTCAT GTCTAACCTT CCTCTACGCA CCGACATAAA AACCCACCTG 
GACCGGCCTT TGGTTGTGGA GCCTCGCTGT GATGGCCCCA TCAGCCCACA CAGTGGCTGG 
GACAAACCCC AGGATTTGCA AGGCGAGGGC AGCACACCAG AAGAACGAGT CCCACCTGTA 
TTCGAGCCTC CACAGTCACA TCCACCACGC AAGCACCACC GTCACAGAGA GCGTCTTTCT 
AATGAGACCA ATGAAAATGG AGACACGGGT CATGCCAAAG AAGGCCGGCA TCACGTTCAT 
CACAGCCGAA GTAAAGAGCA TGATGGGACA CGTTGTAAGG AGGGGAAGGG TGACAGGAGC 
CGAAGCAGAG AAGGGGGACG TAGACATCAC CATCAGAGCT CGGTGGATGA GGGCGTAGGA 
GGTGGAGAGA GGGAACATCG GCACCATCAC TCCCATAGGC ATAACAGAGA GGGCAATGGT 
GTGGTCAGTG CTGGGGGGAA GAGTGAACGG CGGTCCAGGC AGAAAGAGGG CTCACGATCA 
GGGACAAGAG AGGGAGAGAG ATGCTCAAGG GGGGAGAATG GAGGAAATGG AGGGAGGAGG 
CGACACAGGC CACGAACCAG TAAAGCTCAG TCTACGCTGG AAGGAGAGGA GCAACATGTG 
AATGGAGAGG AGGAGGGCAA GTCGCAAAGC CATAGGCACA TGGATATGGG AGGAGATTCA 
ATGGCTGTAA ACCCCCATCA ACCTTCAGTT GGAAGCCGGT GGTGTCTTGA GAAACCAGAA 
GATTCGGACA ACAAACGAAA TATTGGTCAG ACTGGAGGAA TGAGCACCCA TATACCCGTG 
ACTGTCACTT CTCCCCCGGG AGAAACAACC CTTATTCCCA TGAACAGTAT AGACAGCGAG 
ACAGTGCCCA TGACTGAGAA GAATCTAGAA GATCTAAATC AAAGCAGCAT TCGTCCCATC 
CTCCCCTTCA GCTCCATGTT CATTTTCAAC CCAACTAATC CGGTGAGACG TTTATGCCAC 
TACATTGTGA GTCTGCGCTA TTTTGAGATG TGTATCCTGG TGGTCATTGC CATGAGCAGT 
ATAGCCCTGG CTGCAGAAGA CCCGGTCCAA GCCAATGCAC CTCGGAACAA TGTCCTGAAG 
TATTTGGACT ATGTCTTCAC TGGTGTTTTC ACATTCGAGA TGGTCATTAA GATGGTGGAT 
TTAGGTTTAA TTCTGCATCC TGGTTCCTAC TTTCGAGATC TATGGAATAT TTTGGACTTC 
ATTGTGGTCA GTGGTGCACT GGTGGCATTC GCATTCTCGA GCTTCATGGG ATCACATCAA 
AGCGTAAACA GAGGAACCAA GGGCAAAGAC ATCAGCACTA TCAAGTCACT GAGGGTGTTG 
CGAGTCTTGC GACCTCTGAA GACCATCAAA CGTCTACCAA AACTGAAGGC CGTGTTTGAT 
TGTGTGGTGA ACTCCCTGAA GAATGTCCTG AATATCCTCA TTGTCTACAT TCTGTTCATG 
TTCATTTTCG CTGTCATTGC TGTGCAGCTC TTCAAGGGCA AGTTTTTTTA CTGCACAGAC 
GAGTCCAAGG GCCTGGAGAA AGACTGCAGG GGGCTGTTTT TGGATTATGA TAAGGATGAT 
GTGACGGCAC AACCGAGGGA ATGGAAGAAA TACGAGTTTC ACTATGACAA TGTGCTTTGG 
GCTTTTCTCA CTCTCTTCAC TGTTTCTACG GGAGAGGGCT GGCCTACGGT TTTGAAGCAC 
TCCGTGGATG CCACTTTTGA GGATCAGGGT CCCAGTCCAG GCTACCGCAT TGAGATGTCT 
ATTTTTTACG TGGTCTACTT TGTGGTTTTC CCTTTCTTCT TCGTCAACAT TTTTGTAGCC 
TTGATAATCA TCACATTCCA GGAACAGGGT GATAAGGTCT TGTCAGAGTG CAGTCTGGAG 
AAGAATGAGA GAGCCTGCAT CGACTTTGCT ATCAATGCCA AACCTCTGAC ACGCTACATG 
CCGCAGAACC AGCAGTCCTT TCAGTACCGC CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT 
GAGTACTCCA TCATGATCAT GATTGCTCTC AACACTGTGG TGCTCATGAT GAAGTTCCAT 
GGGGCGCCTG ACTTTTATGA GGCAATGCTT AAGTATCTCA ACATTGTCTT CACTGTTCTC 
TTTTCACTGG AGTGTATTCT CAAGATTATT GCTTTCGGCC CCTTGAATTA CCTGAAAGAT 
GCCTGGAATG TGTTTGACTT TGTGACTGTG CTGGGCAGCA TCACTGACAT TTTAGTCACT 
GAGATTAACA CAACGGAGCG TCAGCTGAAC TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC 
CGACTCATCA AGCTCCTCCG GCAGGGATAC ACCATTCGCA TCCTGCTCTG GACATTTGTC 
CAGTCTTTTA AGGCACTGCC TTATGTTTGC CTTCTAATCG CCATGCTGTT TTTCATCTAC 
GCCATTATTG GGATGCAGGT GTTTGGAAAC ATTGATTTGA ATGATGACAC GGCAATCAAC 
CGTCACAACA ACTTCCGCAC TTTCCTCCAA GCTCTTATGC TTTTGTTCAG GAGTGCCACT 
GGCGAGGCTT GGCATGATAT CATGCTGTCG TGTCTGAGCG AGCGCACCTG TGATCCGAGC 
TCAGGCACCC TTGGAAAGGA GTGTGGGAGT GACTTTGCCT ACTTCTACTT TGTCTCCTTC 
ATTTTCCTCT GCTCATTTCT GATGTTGAAC CTGTTTGTGG CCGTCATTAT GGACAACTTT 
GAATATCTGA CACGCGACTC CTCCATCCTG GGCCCTCACC ACCTGGATGA GTTTATTCGA 
GTGTGGGCGG AGTATGACCC AGCCGCATGT GGCCGTATCA AGTACCTCGA TATGTATGAG 
ATGCTGCTCC ACATGTCCCC ACCTTTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC 
TACAAGCGTC TGGTGAAAAT GAACATGCCC ATTGCGGATG ACAACAGTGT ACACTTTACC 
TCAACGCTGA TGGCTCTGAT CCGTACTGCC CTGGAGATCA AACTGGCTTC AGGCATGGTG 
GCTCAGCGTC TGTCAGATGC TGAACTGAAG AAGGAGCTCT CTACAGTTTG GCCAAACCTG 
TCTCAGAAGA CCATGGACCT GCTGGTCACT CCACATAAAC CTAATGAGCT CACAGTGGGG 
AAGGTGTATG CAGCTCTTAT GATCTTTGAC TATTACAAGC AGAACAGAGC AAAAAGACTT 
CAGATGCAGC AACAGCAGCT GAAAGAACAG GCAGGACCTG GATCTCAGAA TAAAGTCAGC 
GCTCTCTTAG AGCCCATTCT CCCACTAACC CACATGCAAG ATCAGCCAGT GAACGGTGTC 
GATTCAGCTT CAGAGTGTCA AGCTCAGACC AGACCCAGCT CCACCACACT CAACAATGGA 
CGCACAACTC TTGACAACAC AATAAAAGGG TCTTCATCCT GGGCCTCAGA GAAATCCAAG 
GAAGTCCAAA AGTCTAAACG GAGACCAATG TCTAGAGGTC AATCTGAGGA TGCCTCAAAT 
GCAAACACTG CCCAGGAGTC AGTAGAAATG AGAAAGATGG AGAACAGTGC CAACACAGTG 
ACCTCTGGTG GCCTTGAGGG CCAAGGACGT GCAGCGTCCA TGCCAAAACT CAATGCTGAG 
ATGCAGAGGA GTCATTCCCG TCAATCTCCT GGAACATTAC TGGAACCCAT TCCAGATTCC 
CCCATGAGGC GCTCTGCATC CACTTTTGCA CCACAGCGCC CTCACGAGGT GAATCTGAAT 
GACTACATTC TGGAGAAACC TGTTCAGGAA CGCCATCACC ACCATCACCA TCATCACCGA 
TGCCATCATC GCCGGGACAG AGACAAGAAA CAGAGATCTC TGGACAGGTC GCCTACTGGC 
CATCACAAAA CTACAGGCAC AGCCACTGAC TCTGTAGAGG AGAGGGCACA TGACCGGGGT 
CGTTCCCATG AGAGGAAGCA CCATTCATCT TCAGGAGAGA AGCAGCGGTA TTACTCGTGT 
GATCGCTATG CCAGTCGGGA ACACTGTTCC AGCAAATCTG CCGTGGCAAG CTGTGCCACC 
TCACCCAGCG AGACCCAGGA GGCCAATCTT AACAAGCAGG GCAGTGGGTG GGCTAAGGGG 
AGCCCGGTGT TGTTGACTTC AGGGGCGAGC ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC 
CAGACCCCCC TCACTCCTCG TCCCTCCGTG GCCTACAGGA CTGCCAACTC TTCCCCTGTG 
CAACTTCTGG CCACAACGAC CAGCTTGCTT CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT 
CTGTCCGAGC ACAACGCTCT TTTACTCAGC GGCTCTATCT CCAGCTCGCC GGCCCCCGTC 
AGTCGCATTA ACTCAGAACC CTTCTTGGGG CCCCCGGACC ACCCTGCTGG CTTGCAGCCC 
GAAGCCTTCG CTGTCTTCCA AGAGAATCCG AGCCCTCAGA TGGGCCGTTC TCCTCAGACT 
GTAGGGATGC CCTTCGTGGC GATCCCCCCA CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC 
AACGGGTACC ACTTCTCCAC GGGCCCCAGC GCCAGCAGAG GACGGGCACA GCAGTACTAC 
CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211584.1
CDS	636..7745
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITASYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_017356095.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356095.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CGAGTTATGT CTGCTCCCCT 
GCACATCACT ACTTGAGTAA AGAACAGCAG CGTTCTCTAA AAATGATGTC CGTGTGGGAG 
CAGCGCACCA CTCAGATCCG AAAGCACATG CTAGCCAGCA GTGAGGCGTT GTACCAAGAA 
GAGCTCAATC CACGGCTCAT GTCTAACCTT CCTCTACGCA CCGACATAAA AACCCACCTG 
GACCGGCCTT TGGTTGTGGA GCCTCGCTGT GATGGCCCCA TCAGCCCACA CAGTGGCTGG 
GACAAACCCC AGGATTTGCA AGGCGAGGGC AGCACACCAG AAGAACGAGT CCCACCTGTA 
TTCGAGCCTC CACAGTCACA TCCACCACGC AAGCACCACC GTCACAGAGA GCGTCTTTCT 
AATGAGACCA ATGAAAATGG AGACACGGGT CATGCCAAAG AAGGCCGGCA TCACGTTCAT 
CACAGCCGAA GTAAAGAGCA TGATGGGACA CGTTGTAAGG AGGGGAAGGG TGACAGGAGC 
CGAAGCAGAG AAGGGGGACG TAGACATCAC CATCAGAGCT CGGTGGATGA GGGCGTAGGA 
GGTGGAGAGA GGGAACATCG GCACCATCAC TCCCATAGGC ATAACAGAGA GGGCAATGGT 
GTGGTCAGTG CTGGGGGGAA GAGTGAACGG CGGTCCAGGC AGAAAGAGGG CTCACGATCA 
GGGACAAGAG AGGGAGAGAG ATGCTCAAGG GGGGAGAATG GAGGAAATGG AGGGAGGAGG 
CGACACAGGC CACGAACCAG TAAAGCTCAG TCTACGCTGG AAGGAGAGGA GCAACATGTG 
AATGGAGAGG AGGAGGGCAA GTCGCAAAGC CATAGGCACA TGGATATGGG AGGAGATTCA 
ATGGCTGTAA ACCCCCATCA ACCTTCAGTT GGAAGCCGGT GGTGTCTTGA GAAACCAGAA 
GATTCGGACA ACAAACGAAA TATTGGTCAG ACTGGAGGAA TGAGCACCCA TATACCCGTG 
ACTGTCACTT CTCCCCCGGG AGAAACAACC CTTATTCCCA TGAACAGTAT AGACAGCGAG 
ACAGTGCCCA TGACTGAGAA GAATCTAGAA GATCTAAATC AAAGCAGCAT TCGTCCCATC 
CTCCCCTTCA GCTCCATGTT CATTTTCAAC CCAACTAATC CGGTGAGACG TTTATGCCAC 
TACATTGTGA GTCTGCGCTA TTTTGAGATG TGTATCCTGG TGGTCATTGC CATGAGCAGT 
ATAGCCCTGG CTGCAGAAGA CCCGGTCCAA GCCAATGCAC CTCGGAACAA TGTCCTGAAG 
TATTTGGACT ATGTCTTCAC TGGTGTTTTC ACATTCGAGA TGGTCATTAA GATGGTGGAT 
TTAGGTTTAA TTCTGCATCC TGGTTCCTAC TTTCGAGATC TATGGAATAT TTTGGACTTC 
ATTGTGGTCA GTGGTGCACT GGTGGCATTC GCATTCTCGA GCTTCATGGG ATCACATCAA 
AGCGTAAACA GAGGAACCAA GGGCAAAGAC ATCAGCACTA TCAAGTCACT GAGGGTGTTG 
CGAGTCTTGC GACCTCTGAA GACCATCAAA CGTCTACCAA AACTGAAGGC CGTGTTTGAT 
TGTGTGGTGA ACTCCCTGAA GAATGTCCTG AATATCCTCA TTGTCTACAT TCTGTTCATG 
TTCATTTTCG CTGTCATTGC TGTGCAGCTC TTCAAGGGCA AGTTTTTTTA CTGCACAGAC 
GAGTCCAAGG GCCTGGAGAA AGACTGCAGG GGGCTGTTTT TGGATTATGA TAAGGATGAT 
GTGACGGCAC AACCGAGGGA ATGGAAGAAA TACGAGTTTC ACTATGACAA TGTGCTTTGG 
GCTTTTCTCA CTCTCTTCAC TGTTTCTACG GGAGAGGGCT GGCCTACGGT TTTGAAGCAC 
TCCGTGGATG CCACTTTTGA GGATCAGGGT CCCAGTCCAG GCTACCGCAT TGAGATGTCT 
ATTTTTTACG TGGTCTACTT TGTGGTTTTC CCTTTCTTCT TCGTCAACAT TTTTGTAGCC 
TTGATAATCA TCACATTCCA GGAACAGGGT GATAAGGTCT TGTCAGAGTG CAGTCTGGAG 
AAGAATGAGA GAGCCTGCAT CGACTTTGCT ATCAATGCCA AACCTCTGAC ACGCTACATG 
CCGCAGAACC AGCAGTCCTT TCAGTACCGC CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT 
GAGTACTCCA TCATGATCAT GATTGCTCTC AACACTGTGG TGCTCATGAT GAAGTTCCAT 
GGGGCGCCTG ACTTTTATGA GGCAATGCTT AAGTATCTCA ACATTGTCTT CACTGTTCTC 
TTTTCACTGG AGTGTATTCT CAAGATTATT GCTTTCGGCC CCTTGAATTA CCTGAAAGAT 
GCCTGGAATG TGTTTGACTT TGTGACTGTG CTGGGCAGCA TCACTGACAT TTTAGTCACT 
GAGATTAACA CAACGGAGCG TCAGCTGAAC TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC 
CGACTCATCA AGCTCCTCCG GCAGGGATAC ACCATTCGCA TCCTGCTCTG GACATTTGTC 
CAGTCTTTTA AGGCACTGCC TTATGTTTGC CTTCTAATCG CCATGCTGTT TTTCATCTAC 
GCCATTATTG GGATGCAGGT GTTTGGAAAC ATTGATTTGA ATGATGACAC GGCAATCAAC 
CGTCACAACA ACTTCCGCAC TTTCCTCCAA GCTCTTATGC TTTTGTTCAG GAGTGCCACT 
GGCGAGGCTT GGCATGATAT CATGCTGTCG TGTCTGAGCG AGCGCACCTG TGATCCGAGC 
TCAGGCACCC TTGGAAAGGA GTGTGGGAGT GACTTTGCCT ACTTCTACTT TGTCTCCTTC 
ATTTTCCTCT GCTCATTTCT GATGTTGAAC CTGTTTGTGG CCGTCATTAT GGACAACTTT 
GAATATCTGA CACGCGACTC CTCCATCCTG GGCCCTCACC ACCTGGATGA GTTTATTCGA 
GTGTGGGCGG AGTATGACCC AGCCGCATGT GGCCGTATCA AGTACCTCGA TATGTATGAG 
ATGCTGCTCC ACATGTCCCC ACCTTTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC 
TACAAGCGTC TGGTGAAAAT GAACATGCCC ATTGCGGATG ACAACAGTGT ACACTTTACC 
TCAACGCTGA TGGCTCTGAT CCGTACTGCC CTGGAGATCA AACTGGCTTC AGGCATGGTG 
GCTCAGCGTC TGTCAGATGC TGAACTGAAG AAGGAGCTCT CTACAGTTTG GCCAAACCTG 
TCTCAGAAGA CCATGGACCT GCTGGTCACT CCACATAAAC CTAATGAGCT CACAGTGGGG 
AAGGTGTATG CAGCTCTTAT GATCTTTGAC TATTACAAGC AGAACAGAGC AAAAAGACTT 
CAGATGCAGC AACAGCAGCT GAAAGAACAG GCAGGACCTG GATCTCAGAA TAAAGTCAGC 
GCTCTCTTAG AGCCCATTCT CCCACTAACC CACATGCAAG ATCAGCCAGT GAACGGTGTC 
GATTCAGCTT CAGAGTGTCA AGCTCAGACC AGACCCAGCT CCACCACACT CAACAATGGA 
CGCACAACTC TTGACAACAC AATAAAAGGG TCTTCATCCT GGGCCTCAGA GAAATCCAAG 
GAAGTCCAAA AGTCTAAACG GAGACCAATG TCTAGAGGTC AATCTGAGGA TGCCTCAAAT 
GCAAACACTG CCCAGGAGTC AGTAGAAATG AGAAAGATGG AGAACAGTGC CAACACAGTG 
ACCTCTGGTG GCCTTGAGGG CCAAGGACGT GCAGCGTCCA TGCCAAAACT CAATGCTGAG 
ATGCAGAGGA GTCATTCCCG TCAATCTCCT GGAACATTAC TGGAACCCAT TCCAGATTCC 
CCCATGAGGC GCTCTGCATC CACTTTTGCA CCACAGCGCC CTCACGAGGT GAATCTGAAT 
GACTACATTC TGGAGAAACC TGTTCAGGAA CGCCATCACC ACCATCACCA TCATCACCGA 
TGCCATCATC GCCGGGACAG AGACAAGAAA CAGAGATCTC TGGACAGGTC GCCTACTGGC 
CATCACAAAA CTACAGGCAC AGCCACTGAC TCTGTAGAGG AGAGGGCACA TGACCGGGGT 
CGTTCCCATG AGAGGAAGCA CCATTCATCT TCAGGAGAGA AGCAGCGGTA TTACTCGTGT 
GATCGCTATG CCAGTCGGGA ACACTGTTCC AGCAAATCTG CCGTGGCAAG CTGTGCCACC 
TCACCCAGCG AGACCCAGGA GGCCAATCTT AACAAGCAGG GCAGTGGGTG GGCTAAGGGG 
AGCCCGGTGT TGTTGACTTC AGGGGCGAGC ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC 
CAGACCCCCC TCACTCCTCG TCCCTCCGTG GCCTACAGGA CTGCCAACTC TTCCCCTGTG 
CAACTTCTGG CCACAACGAC CAGCTTGCTT CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT 
CTGTCCGAGC ACAACGCTCT TTTACTCAGC GGCTCTATCT CCAGCTCGCC GGCCCCCGTC 
AGTCGCATTA ACTCAGAACC CTTCTTGGGG CCCCCGGACC ACCCTGCTGG CTTGCAGCCC 
GAAGCCTTCG CTGTCTTCCA AGAGAATCCG AGCCCTCAGA TGGGCCGTTC TCCTCAGACT 
GTAGGGATGC CCTTCGTGGC GATCCCCCCA CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC 
AACGGGTACC ACTTCTCCAC GGGCCCCAGC GCCAGCAGAG GACGGGCACA GCAGTACTAC 
CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_017356095.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211584.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA.

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CloneID	ODa59085
Clone ID Related Accession (Same CDS sequence)	XM_017356095.1 , XM_017356095.2
Accession Version	XM_017356095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CGAGTTATGT CTGCTCCCCT 
GCACATCACT ACTTGAGTAA AGAACAGCAG CGTTCTCTAA AAATGATGTC CGTGTGGGAG 
CAGCGCACCA CTCAGATCCG AAAGCACATG CTAGCCAGCA GTGAGGCGTT GTACCAAGAA 
GAGCTCAATC CACGGCTCAT GTCTAACCTT CCTCTACGCA CCGACATAAA AACCCACCTG 
GACCGGCCTT TGGTTGTGGA GCCTCGCTGT GATGGCCCCA TCAGCCCACA CAGTGGCTGG 
GACAAACCCC AGGATTTGCA AGGCGAGGGC AGCACACCAG AAGAACGAGT CCCACCTGTA 
TTCGAGCCTC CACAGTCACA TCCACCACGC AAGCACCACC GTCACAGAGA GCGTCTTTCT 
AATGAGACCA ATGAAAATGG AGACACGGGT CATGCCAAAG AAGGCCGGCA TCACGTTCAT 
CACAGCCGAA GTAAAGAGCA TGATGGGACA CGTTGTAAGG AGGGGAAGGG TGACAGGAGC 
CGAAGCAGAG AAGGGGGACG TAGACATCAC CATCAGAGCT CGGTGGATGA GGGCGTAGGA 
GGTGGAGAGA GGGAACATCG GCACCATCAC TCCCATAGGC ATAACAGAGA GGGCAATGGT 
GTGGTCAGTG CTGGGGGGAA GAGTGAACGG CGGTCCAGGC AGAAAGAGGG CTCACGATCA 
GGGACAAGAG AGGGAGAGAG ATGCTCAAGG GGGGAGAATG GAGGAAATGG AGGGAGGAGG 
CGACACAGGC CACGAACCAG TAAAGCTCAG TCTACGCTGG AAGGAGAGGA GCAACATGTG 
AATGGAGAGG AGGAGGGCAA GTCGCAAAGC CATAGGCACA TGGATATGGG AGGAGATTCA 
ATGGCTGTAA ACCCCCATCA ACCTTCAGTT GGAAGCCGGT GGTGTCTTGA GAAACCAGAA 
GATTCGGACA ACAAACGAAA TATTGGTCAG ACTGGAGGAA TGAGCACCCA TATACCCGTG 
ACTGTCACTT CTCCCCCGGG AGAAACAACC CTTATTCCCA TGAACAGTAT AGACAGCGAG 
ACAGTGCCCA TGACTGAGAA GAATCTAGAA GATCTAAATC AAAGCAGCAT TCGTCCCATC 
CTCCCCTTCA GCTCCATGTT CATTTTCAAC CCAACTAATC CGGTGAGACG TTTATGCCAC 
TACATTGTGA GTCTGCGCTA TTTTGAGATG TGTATCCTGG TGGTCATTGC CATGAGCAGT 
ATAGCCCTGG CTGCAGAAGA CCCGGTCCAA GCCAATGCAC CTCGGAACAA TGTCCTGAAG 
TATTTGGACT ATGTCTTCAC TGGTGTTTTC ACATTCGAGA TGGTCATTAA GATGGTGGAT 
TTAGGTTTAA TTCTGCATCC TGGTTCCTAC TTTCGAGATC TATGGAATAT TTTGGACTTC 
ATTGTGGTCA GTGGTGCACT GGTGGCATTC GCATTCTCGA GCTTCATGGG ATCACATCAA 
AGCGTAAACA GAGGAACCAA GGGCAAAGAC ATCAGCACTA TCAAGTCACT GAGGGTGTTG 
CGAGTCTTGC GACCTCTGAA GACCATCAAA CGTCTACCAA AACTGAAGGC CGTGTTTGAT 
TGTGTGGTGA ACTCCCTGAA GAATGTCCTG AATATCCTCA TTGTCTACAT TCTGTTCATG 
TTCATTTTCG CTGTCATTGC TGTGCAGCTC TTCAAGGGCA AGTTTTTTTA CTGCACAGAC 
GAGTCCAAGG GCCTGGAGAA AGACTGCAGG GGGCTGTTTT TGGATTATGA TAAGGATGAT 
GTGACGGCAC AACCGAGGGA ATGGAAGAAA TACGAGTTTC ACTATGACAA TGTGCTTTGG 
GCTTTTCTCA CTCTCTTCAC TGTTTCTACG GGAGAGGGCT GGCCTACGGT TTTGAAGCAC 
TCCGTGGATG CCACTTTTGA GGATCAGGGT CCCAGTCCAG GCTACCGCAT TGAGATGTCT 
ATTTTTTACG TGGTCTACTT TGTGGTTTTC CCTTTCTTCT TCGTCAACAT TTTTGTAGCC 
TTGATAATCA TCACATTCCA GGAACAGGGT GATAAGGTCT TGTCAGAGTG CAGTCTGGAG 
AAGAATGAGA GAGCCTGCAT CGACTTTGCT ATCAATGCCA AACCTCTGAC ACGCTACATG 
CCGCAGAACC AGCAGTCCTT TCAGTACCGC CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT 
GAGTACTCCA TCATGATCAT GATTGCTCTC AACACTGTGG TGCTCATGAT GAAGTTCCAT 
GGGGCGCCTG ACTTTTATGA GGCAATGCTT AAGTATCTCA ACATTGTCTT CACTGTTCTC 
TTTTCACTGG AGTGTATTCT CAAGATTATT GCTTTCGGCC CCTTGAATTA CCTGAAAGAT 
GCCTGGAATG TGTTTGACTT TGTGACTGTG CTGGGCAGCA TCACTGACAT TTTAGTCACT 
GAGATTAACA CAACGGAGCG TCAGCTGAAC TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC 
CGACTCATCA AGCTCCTCCG GCAGGGATAC ACCATTCGCA TCCTGCTCTG GACATTTGTC 
CAGTCTTTTA AGGCACTGCC TTATGTTTGC CTTCTAATCG CCATGCTGTT TTTCATCTAC 
GCCATTATTG GGATGCAGGT GTTTGGAAAC ATTGATTTGA ATGATGACAC GGCAATCAAC 
CGTCACAACA ACTTCCGCAC TTTCCTCCAA GCTCTTATGC TTTTGTTCAG GAGTGCCACT 
GGCGAGGCTT GGCATGATAT CATGCTGTCG TGTCTGAGCG AGCGCACCTG TGATCCGAGC 
TCAGGCACCC TTGGAAAGGA GTGTGGGAGT GACTTTGCCT ACTTCTACTT TGTCTCCTTC 
ATTTTCCTCT GCTCATTTCT GATGTTGAAC CTGTTTGTGG CCGTCATTAT GGACAACTTT 
GAATATCTGA CACGCGACTC CTCCATCCTG GGCCCTCACC ACCTGGATGA GTTTATTCGA 
GTGTGGGCGG AGTATGACCC AGCCGCATGT GGCCGTATCA AGTACCTCGA TATGTATGAG 
ATGCTGCTCC ACATGTCCCC ACCTTTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC 
TACAAGCGTC TGGTGAAAAT GAACATGCCC ATTGCGGATG ACAACAGTGT ACACTTTACC 
TCAACGCTGA TGGCTCTGAT CCGTACTGCC CTGGAGATCA AACTGGCTTC AGGCATGGTG 
GCTCAGCGTC TGTCAGATGC TGAACTGAAG AAGGAGCTCT CTACAGTTTG GCCAAACCTG 
TCTCAGAAGA CCATGGACCT GCTGGTCACT CCACATAAAC CTAATGAGCT CACAGTGGGG 
AAGGTGTATG CAGCTCTTAT GATCTTTGAC TATTACAAGC AGAACAGAGC AAAAAGACTT 
CAGATGCAGC AACAGCAGCT GAAAGAACAG GCAGGACCTG GATCTCAGAA TAAAGTCAGC 
GCTCTCTTAG AGCCCATTCT CCCACTAACC CACATGCAAG ATCAGCCAGT GAACGGTGTC 
GATTCAGCTT CAGAGTGTCA AGCTCAGACC AGACCCAGCT CCACCACACT CAACAATGGA 
CGCACAACTC TTGACAACAC AATAAAAGGG TCTTCATCCT GGGCCTCAGA GAAATCCAAG 
GAAGTCCAAA AGTCTAAACG GAGACCAATG TCTAGAGGTC AATCTGAGGA TGCCTCAAAT 
GCAAACACTG CCCAGGAGTC AGTAGAAATG AGAAAGATGG AGAACAGTGC CAACACAGTG 
ACCTCTGGTG GCCTTGAGGG CCAAGGACGT GCAGCGTCCA TGCCAAAACT CAATGCTGAG 
ATGCAGAGGA GTCATTCCCG TCAATCTCCT GGAACATTAC TGGAACCCAT TCCAGATTCC 
CCCATGAGGC GCTCTGCATC CACTTTTGCA CCACAGCGCC CTCACGAGGT GAATCTGAAT 
GACTACATTC TGGAGAAACC TGTTCAGGAA CGCCATCACC ACCATCACCA TCATCACCGA 
TGCCATCATC GCCGGGACAG AGACAAGAAA CAGAGATCTC TGGACAGGTC GCCTACTGGC 
CATCACAAAA CTACAGGCAC AGCCACTGAC TCTGTAGAGG AGAGGGCACA TGACCGGGGT 
CGTTCCCATG AGAGGAAGCA CCATTCATCT TCAGGAGAGA AGCAGCGGTA TTACTCGTGT 
GATCGCTATG CCAGTCGGGA ACACTGTTCC AGCAAATCTG CCGTGGCAAG CTGTGCCACC 
TCACCCAGCG AGACCCAGGA GGCCAATCTT AACAAGCAGG GCAGTGGGTG GGCTAAGGGG 
AGCCCGGTGT TGTTGACTTC AGGGGCGAGC ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC 
CAGACCCCCC TCACTCCTCG TCCCTCCGTG GCCTACAGGA CTGCCAACTC TTCCCCTGTG 
CAACTTCTGG CCACAACGAC CAGCTTGCTT CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT 
CTGTCCGAGC ACAACGCTCT TTTACTCAGC GGCTCTATCT CCAGCTCGCC GGCCCCCGTC 
AGTCGCATTA ACTCAGAACC CTTCTTGGGG CCCCCGGACC ACCCTGCTGG CTTGCAGCCC 
GAAGCCTTCG CTGTCTTCCA AGAGAATCCG AGCCCTCAGA TGGGCCGTTC TCCTCAGACT 
GTAGGGATGC CCTTCGTGGC GATCCCCCCA CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC 
AACGGGTACC ACTTCTCCAC GGGCCCCAGC GCCAGCAGAG GACGGGCACA GCAGTACTAC 
CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211584.1
CDS	1..7110
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITASYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_017356095.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356095.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CGAGTTATGT CTGCTCCCCT 
GCACATCACT ACTTGAGTAA AGAACAGCAG CGTTCTCTAA AAATGATGTC CGTGTGGGAG 
CAGCGCACCA CTCAGATCCG AAAGCACATG CTAGCCAGCA GTGAGGCGTT GTACCAAGAA 
GAGCTCAATC CACGGCTCAT GTCTAACCTT CCTCTACGCA CCGACATAAA AACCCACCTG 
GACCGGCCTT TGGTTGTGGA GCCTCGCTGT GATGGCCCCA TCAGCCCACA CAGTGGCTGG 
GACAAACCCC AGGATTTGCA AGGCGAGGGC AGCACACCAG AAGAACGAGT CCCACCTGTA 
TTCGAGCCTC CACAGTCACA TCCACCACGC AAGCACCACC GTCACAGAGA GCGTCTTTCT 
AATGAGACCA ATGAAAATGG AGACACGGGT CATGCCAAAG AAGGCCGGCA TCACGTTCAT 
CACAGCCGAA GTAAAGAGCA TGATGGGACA CGTTGTAAGG AGGGGAAGGG TGACAGGAGC 
CGAAGCAGAG AAGGGGGACG TAGACATCAC CATCAGAGCT CGGTGGATGA GGGCGTAGGA 
GGTGGAGAGA GGGAACATCG GCACCATCAC TCCCATAGGC ATAACAGAGA GGGCAATGGT 
GTGGTCAGTG CTGGGGGGAA GAGTGAACGG CGGTCCAGGC AGAAAGAGGG CTCACGATCA 
GGGACAAGAG AGGGAGAGAG ATGCTCAAGG GGGGAGAATG GAGGAAATGG AGGGAGGAGG 
CGACACAGGC CACGAACCAG TAAAGCTCAG TCTACGCTGG AAGGAGAGGA GCAACATGTG 
AATGGAGAGG AGGAGGGCAA GTCGCAAAGC CATAGGCACA TGGATATGGG AGGAGATTCA 
ATGGCTGTAA ACCCCCATCA ACCTTCAGTT GGAAGCCGGT GGTGTCTTGA GAAACCAGAA 
GATTCGGACA ACAAACGAAA TATTGGTCAG ACTGGAGGAA TGAGCACCCA TATACCCGTG 
ACTGTCACTT CTCCCCCGGG AGAAACAACC CTTATTCCCA TGAACAGTAT AGACAGCGAG 
ACAGTGCCCA TGACTGAGAA GAATCTAGAA GATCTAAATC AAAGCAGCAT TCGTCCCATC 
CTCCCCTTCA GCTCCATGTT CATTTTCAAC CCAACTAATC CGGTGAGACG TTTATGCCAC 
TACATTGTGA GTCTGCGCTA TTTTGAGATG TGTATCCTGG TGGTCATTGC CATGAGCAGT 
ATAGCCCTGG CTGCAGAAGA CCCGGTCCAA GCCAATGCAC CTCGGAACAA TGTCCTGAAG 
TATTTGGACT ATGTCTTCAC TGGTGTTTTC ACATTCGAGA TGGTCATTAA GATGGTGGAT 
TTAGGTTTAA TTCTGCATCC TGGTTCCTAC TTTCGAGATC TATGGAATAT TTTGGACTTC 
ATTGTGGTCA GTGGTGCACT GGTGGCATTC GCATTCTCGA GCTTCATGGG ATCACATCAA 
AGCGTAAACA GAGGAACCAA GGGCAAAGAC ATCAGCACTA TCAAGTCACT GAGGGTGTTG 
CGAGTCTTGC GACCTCTGAA GACCATCAAA CGTCTACCAA AACTGAAGGC CGTGTTTGAT 
TGTGTGGTGA ACTCCCTGAA GAATGTCCTG AATATCCTCA TTGTCTACAT TCTGTTCATG 
TTCATTTTCG CTGTCATTGC TGTGCAGCTC TTCAAGGGCA AGTTTTTTTA CTGCACAGAC 
GAGTCCAAGG GCCTGGAGAA AGACTGCAGG GGGCTGTTTT TGGATTATGA TAAGGATGAT 
GTGACGGCAC AACCGAGGGA ATGGAAGAAA TACGAGTTTC ACTATGACAA TGTGCTTTGG 
GCTTTTCTCA CTCTCTTCAC TGTTTCTACG GGAGAGGGCT GGCCTACGGT TTTGAAGCAC 
TCCGTGGATG CCACTTTTGA GGATCAGGGT CCCAGTCCAG GCTACCGCAT TGAGATGTCT 
ATTTTTTACG TGGTCTACTT TGTGGTTTTC CCTTTCTTCT TCGTCAACAT TTTTGTAGCC 
TTGATAATCA TCACATTCCA GGAACAGGGT GATAAGGTCT TGTCAGAGTG CAGTCTGGAG 
AAGAATGAGA GAGCCTGCAT CGACTTTGCT ATCAATGCCA AACCTCTGAC ACGCTACATG 
CCGCAGAACC AGCAGTCCTT TCAGTACCGC CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT 
GAGTACTCCA TCATGATCAT GATTGCTCTC AACACTGTGG TGCTCATGAT GAAGTTCCAT 
GGGGCGCCTG ACTTTTATGA GGCAATGCTT AAGTATCTCA ACATTGTCTT CACTGTTCTC 
TTTTCACTGG AGTGTATTCT CAAGATTATT GCTTTCGGCC CCTTGAATTA CCTGAAAGAT 
GCCTGGAATG TGTTTGACTT TGTGACTGTG CTGGGCAGCA TCACTGACAT TTTAGTCACT 
GAGATTAACA CAACGGAGCG TCAGCTGAAC TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC 
CGACTCATCA AGCTCCTCCG GCAGGGATAC ACCATTCGCA TCCTGCTCTG GACATTTGTC 
CAGTCTTTTA AGGCACTGCC TTATGTTTGC CTTCTAATCG CCATGCTGTT TTTCATCTAC 
GCCATTATTG GGATGCAGGT GTTTGGAAAC ATTGATTTGA ATGATGACAC GGCAATCAAC 
CGTCACAACA ACTTCCGCAC TTTCCTCCAA GCTCTTATGC TTTTGTTCAG GAGTGCCACT 
GGCGAGGCTT GGCATGATAT CATGCTGTCG TGTCTGAGCG AGCGCACCTG TGATCCGAGC 
TCAGGCACCC TTGGAAAGGA GTGTGGGAGT GACTTTGCCT ACTTCTACTT TGTCTCCTTC 
ATTTTCCTCT GCTCATTTCT GATGTTGAAC CTGTTTGTGG CCGTCATTAT GGACAACTTT 
GAATATCTGA CACGCGACTC CTCCATCCTG GGCCCTCACC ACCTGGATGA GTTTATTCGA 
GTGTGGGCGG AGTATGACCC AGCCGCATGT GGCCGTATCA AGTACCTCGA TATGTATGAG 
ATGCTGCTCC ACATGTCCCC ACCTTTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC 
TACAAGCGTC TGGTGAAAAT GAACATGCCC ATTGCGGATG ACAACAGTGT ACACTTTACC 
TCAACGCTGA TGGCTCTGAT CCGTACTGCC CTGGAGATCA AACTGGCTTC AGGCATGGTG 
GCTCAGCGTC TGTCAGATGC TGAACTGAAG AAGGAGCTCT CTACAGTTTG GCCAAACCTG 
TCTCAGAAGA CCATGGACCT GCTGGTCACT CCACATAAAC CTAATGAGCT CACAGTGGGG 
AAGGTGTATG CAGCTCTTAT GATCTTTGAC TATTACAAGC AGAACAGAGC AAAAAGACTT 
CAGATGCAGC AACAGCAGCT GAAAGAACAG GCAGGACCTG GATCTCAGAA TAAAGTCAGC 
GCTCTCTTAG AGCCCATTCT CCCACTAACC CACATGCAAG ATCAGCCAGT GAACGGTGTC 
GATTCAGCTT CAGAGTGTCA AGCTCAGACC AGACCCAGCT CCACCACACT CAACAATGGA 
CGCACAACTC TTGACAACAC AATAAAAGGG TCTTCATCCT GGGCCTCAGA GAAATCCAAG 
GAAGTCCAAA AGTCTAAACG GAGACCAATG TCTAGAGGTC AATCTGAGGA TGCCTCAAAT 
GCAAACACTG CCCAGGAGTC AGTAGAAATG AGAAAGATGG AGAACAGTGC CAACACAGTG 
ACCTCTGGTG GCCTTGAGGG CCAAGGACGT GCAGCGTCCA TGCCAAAACT CAATGCTGAG 
ATGCAGAGGA GTCATTCCCG TCAATCTCCT GGAACATTAC TGGAACCCAT TCCAGATTCC 
CCCATGAGGC GCTCTGCATC CACTTTTGCA CCACAGCGCC CTCACGAGGT GAATCTGAAT 
GACTACATTC TGGAGAAACC TGTTCAGGAA CGCCATCACC ACCATCACCA TCATCACCGA 
TGCCATCATC GCCGGGACAG AGACAAGAAA CAGAGATCTC TGGACAGGTC GCCTACTGGC 
CATCACAAAA CTACAGGCAC AGCCACTGAC TCTGTAGAGG AGAGGGCACA TGACCGGGGT 
CGTTCCCATG AGAGGAAGCA CCATTCATCT TCAGGAGAGA AGCAGCGGTA TTACTCGTGT 
GATCGCTATG CCAGTCGGGA ACACTGTTCC AGCAAATCTG CCGTGGCAAG CTGTGCCACC 
TCACCCAGCG AGACCCAGGA GGCCAATCTT AACAAGCAGG GCAGTGGGTG GGCTAAGGGG 
AGCCCGGTGT TGTTGACTTC AGGGGCGAGC ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC 
CAGACCCCCC TCACTCCTCG TCCCTCCGTG GCCTACAGGA CTGCCAACTC TTCCCCTGTG 
CAACTTCTGG CCACAACGAC CAGCTTGCTT CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT 
CTGTCCGAGC ACAACGCTCT TTTACTCAGC GGCTCTATCT CCAGCTCGCC GGCCCCCGTC 
AGTCGCATTA ACTCAGAACC CTTCTTGGGG CCCCCGGACC ACCCTGCTGG CTTGCAGCCC 
GAAGCCTTCG CTGTCTTCCA AGAGAATCCG AGCCCTCAGA TGGGCCGTTC TCCTCAGACT 
GTAGGGATGC CCTTCGTGGC GATCCCCCCA CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC 
AACGGGTACC ACTTCTCCAC GGGCCCCAGC GCCAGCAGAG GACGGGCACA GCAGTACTAC 
CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_017356096.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211585.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA.

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CloneID	ODa59086
Clone ID Related Accession (Same CDS sequence)	XM_017356096.2 , XM_017356096.1
Accession Version	XM_017356096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG 
ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG 
CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT 
GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT 
TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG 
TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA 
AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA 
GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG 
GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA 
CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG 
GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA 
GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA 
ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG 
GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC 
CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA 
CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC 
CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT 
GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC 
ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG 
CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA 
GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC 
TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG 
CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT 
GCACTGGTGG CATTCGCATT CTCGAGCTTC ATGGGATCAC ATCAAAGCGT AAACAGAGGA 
ACCAAGGGCA AAGACATCAG CACTATCAAG TCACTGAGGG TGTTGCGAGT CTTGCGACCT 
CTGAAGACCA TCAAACGTCT ACCAAAACTG AAGGCCGTGT TTGATTGTGT GGTGAACTCC 
CTGAAGAATG TCCTGAATAT CCTCATTGTC TACATTCTGT TCATGTTCAT TTTCGCTGTC 
ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT TTTTACTGCA CAGACGAGTC CAAGGGCCTG 
GAGAAAGACT GCAGGGGGCT GTTTTTGGAT TATGATAAGG ATGATGTGAC GGCACAACCG 
AGGGAATGGA AGAAATACGA GTTTCACTAT GACAATGTGC TTTGGGCTTT TCTCACTCTC 
TTCACTGTTT CTACGGGAGA GGGCTGGCCT ACGGTTTTGA AGCACTCCGT GGATGCCACT 
TTTGAGGATC AGGGTCCCAG TCCAGGCTAC CGCATTGAGA TGTCTATTTT TTACGTGGTC 
TACTTTGTGG TTTTCCCTTT CTTCTTCGTC AACATTTTTG TAGCCTTGAT AATCATCACA 
TTCCAGGAAC AGGGTGATAA GGTCTTGTCA GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC 
TGCATCGACT TTGCTATCAA TGCCAAACCT CTGACACGCT ACATGCCGCA GAACCAGCAG 
TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG GTCTCTCCGC CGTTTGAGTA CTCCATCATG 
ATCATGATTG CTCTCAACAC TGTGGTGCTC ATGATGAAGT TCCATGGGGC GCCTGACTTT 
TATGAGGCAA TGCTTAAGTA TCTCAACATT GTCTTCACTG TTCTCTTTTC ACTGGAGTGT 
ATTCTCAAGA TTATTGCTTT CGGCCCCTTG AATTACCTGA AAGATGCCTG GAATGTGTTT 
GACTTTGTGA CTGTGCTGGG CAGCATCACT GACATTTTAG TCACTGAGAT TAACACAACG 
GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC 
CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA 
CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG 
CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC 
CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT 
GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA 
AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA 
TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC 
GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT 
GACCCAGCCG CATGTGGCCG TATCAAGTAC CTCGATATGT ATGAGATGCT GCTCCACATG 
TCCCCACCTT TAGGTTTGGG AAAGAAATGT CCTCCACGAA TCGCCTACAA GCGTCTGGTG 
AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT 
CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA 
GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG 
GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT 
CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG 
CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC 
ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG 
TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC 
AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT 
AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG 
GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT 
GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT 
TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT 
GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG 
AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG 
GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA 
GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG 
AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT 
CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC 
CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG 
ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT 
CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA 
ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC 
GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA 
GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC 
TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC 
GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC 
TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG 
GAGGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211585.1
CDS	1..7095
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_017356096.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356096.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG 
ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG 
CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT 
GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT 
TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG 
TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA 
AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA 
GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG 
GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA 
CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG 
GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA 
GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA 
ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG 
GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC 
CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA 
CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC 
CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT 
GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC 
ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG 
CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA 
GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC 
TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG 
CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT 
GCACTGGTGG CATTCGCATT CTCGAGCTTC ATGGGATCAC ATCAAAGCGT AAACAGAGGA 
ACCAAGGGCA AAGACATCAG CACTATCAAG TCACTGAGGG TGTTGCGAGT CTTGCGACCT 
CTGAAGACCA TCAAACGTCT ACCAAAACTG AAGGCCGTGT TTGATTGTGT GGTGAACTCC 
CTGAAGAATG TCCTGAATAT CCTCATTGTC TACATTCTGT TCATGTTCAT TTTCGCTGTC 
ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT TTTTACTGCA CAGACGAGTC CAAGGGCCTG 
GAGAAAGACT GCAGGGGGCT GTTTTTGGAT TATGATAAGG ATGATGTGAC GGCACAACCG 
AGGGAATGGA AGAAATACGA GTTTCACTAT GACAATGTGC TTTGGGCTTT TCTCACTCTC 
TTCACTGTTT CTACGGGAGA GGGCTGGCCT ACGGTTTTGA AGCACTCCGT GGATGCCACT 
TTTGAGGATC AGGGTCCCAG TCCAGGCTAC CGCATTGAGA TGTCTATTTT TTACGTGGTC 
TACTTTGTGG TTTTCCCTTT CTTCTTCGTC AACATTTTTG TAGCCTTGAT AATCATCACA 
TTCCAGGAAC AGGGTGATAA GGTCTTGTCA GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC 
TGCATCGACT TTGCTATCAA TGCCAAACCT CTGACACGCT ACATGCCGCA GAACCAGCAG 
TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG GTCTCTCCGC CGTTTGAGTA CTCCATCATG 
ATCATGATTG CTCTCAACAC TGTGGTGCTC ATGATGAAGT TCCATGGGGC GCCTGACTTT 
TATGAGGCAA TGCTTAAGTA TCTCAACATT GTCTTCACTG TTCTCTTTTC ACTGGAGTGT 
ATTCTCAAGA TTATTGCTTT CGGCCCCTTG AATTACCTGA AAGATGCCTG GAATGTGTTT 
GACTTTGTGA CTGTGCTGGG CAGCATCACT GACATTTTAG TCACTGAGAT TAACACAACG 
GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC 
CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA 
CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG 
CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC 
CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT 
GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA 
AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA 
TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC 
GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT 
GACCCAGCCG CATGTGGCCG TATCAAGTAC CTCGATATGT ATGAGATGCT GCTCCACATG 
TCCCCACCTT TAGGTTTGGG AAAGAAATGT CCTCCACGAA TCGCCTACAA GCGTCTGGTG 
AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT 
CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA 
GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG 
GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT 
CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG 
CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC 
ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG 
TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC 
AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT 
AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG 
GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT 
GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT 
TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT 
GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG 
AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG 
GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA 
GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG 
AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT 
CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC 
CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG 
ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT 
CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA 
ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC 
GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA 
GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC 
TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC 
GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC 
TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG 
GAGGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_017356096.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211585.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA.

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CloneID	ODa59086
Clone ID Related Accession (Same CDS sequence)	XM_017356096.2 , XM_017356096.1
Accession Version	XM_017356096.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG 
ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG 
CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT 
GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT 
TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG 
TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA 
AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA 
GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG 
GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA 
CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG 
GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA 
GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA 
ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG 
GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC 
CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA 
CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC 
CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT 
GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC 
ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG 
CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA 
GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC 
TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG 
CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT 
GCACTGGTGG CATTCGCATT CTCGAGCTTC ATGGGATCAC ATCAAAGCGT AAACAGAGGA 
ACCAAGGGCA AAGACATCAG CACTATCAAG TCACTGAGGG TGTTGCGAGT CTTGCGACCT 
CTGAAGACCA TCAAACGTCT ACCAAAACTG AAGGCCGTGT TTGATTGTGT GGTGAACTCC 
CTGAAGAATG TCCTGAATAT CCTCATTGTC TACATTCTGT TCATGTTCAT TTTCGCTGTC 
ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT TTTTACTGCA CAGACGAGTC CAAGGGCCTG 
GAGAAAGACT GCAGGGGGCT GTTTTTGGAT TATGATAAGG ATGATGTGAC GGCACAACCG 
AGGGAATGGA AGAAATACGA GTTTCACTAT GACAATGTGC TTTGGGCTTT TCTCACTCTC 
TTCACTGTTT CTACGGGAGA GGGCTGGCCT ACGGTTTTGA AGCACTCCGT GGATGCCACT 
TTTGAGGATC AGGGTCCCAG TCCAGGCTAC CGCATTGAGA TGTCTATTTT TTACGTGGTC 
TACTTTGTGG TTTTCCCTTT CTTCTTCGTC AACATTTTTG TAGCCTTGAT AATCATCACA 
TTCCAGGAAC AGGGTGATAA GGTCTTGTCA GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC 
TGCATCGACT TTGCTATCAA TGCCAAACCT CTGACACGCT ACATGCCGCA GAACCAGCAG 
TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG GTCTCTCCGC CGTTTGAGTA CTCCATCATG 
ATCATGATTG CTCTCAACAC TGTGGTGCTC ATGATGAAGT TCCATGGGGC GCCTGACTTT 
TATGAGGCAA TGCTTAAGTA TCTCAACATT GTCTTCACTG TTCTCTTTTC ACTGGAGTGT 
ATTCTCAAGA TTATTGCTTT CGGCCCCTTG AATTACCTGA AAGATGCCTG GAATGTGTTT 
GACTTTGTGA CTGTGCTGGG CAGCATCACT GACATTTTAG TCACTGAGAT TAACACAACG 
GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC 
CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA 
CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG 
CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC 
CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT 
GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA 
AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA 
TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC 
GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT 
GACCCAGCCG CATGTGGCCG TATCAAGTAC CTCGATATGT ATGAGATGCT GCTCCACATG 
TCCCCACCTT TAGGTTTGGG AAAGAAATGT CCTCCACGAA TCGCCTACAA GCGTCTGGTG 
AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT 
CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA 
GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG 
GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT 
CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG 
CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC 
ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG 
TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC 
AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT 
AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG 
GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT 
GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT 
TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT 
GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG 
AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG 
GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA 
GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG 
AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT 
CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC 
CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG 
ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT 
CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA 
ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC 
GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA 
GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC 
TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC 
GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC 
TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG 
GAGGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211585.1
CDS	636..7730
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_017356096.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356096.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG 
ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG 
CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT 
GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT 
TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG 
TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA 
AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA 
GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG 
GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA 
CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG 
GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA 
GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA 
ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG 
GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC 
CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA 
CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC 
CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT 
GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC 
ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG 
CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA 
GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC 
TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG 
CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT 
GCACTGGTGG CATTCGCATT CTCGAGCTTC ATGGGATCAC ATCAAAGCGT AAACAGAGGA 
ACCAAGGGCA AAGACATCAG CACTATCAAG TCACTGAGGG TGTTGCGAGT CTTGCGACCT 
CTGAAGACCA TCAAACGTCT ACCAAAACTG AAGGCCGTGT TTGATTGTGT GGTGAACTCC 
CTGAAGAATG TCCTGAATAT CCTCATTGTC TACATTCTGT TCATGTTCAT TTTCGCTGTC 
ATTGCTGTGC AGCTCTTCAA GGGCAAGTTT TTTTACTGCA CAGACGAGTC CAAGGGCCTG 
GAGAAAGACT GCAGGGGGCT GTTTTTGGAT TATGATAAGG ATGATGTGAC GGCACAACCG 
AGGGAATGGA AGAAATACGA GTTTCACTAT GACAATGTGC TTTGGGCTTT TCTCACTCTC 
TTCACTGTTT CTACGGGAGA GGGCTGGCCT ACGGTTTTGA AGCACTCCGT GGATGCCACT 
TTTGAGGATC AGGGTCCCAG TCCAGGCTAC CGCATTGAGA TGTCTATTTT TTACGTGGTC 
TACTTTGTGG TTTTCCCTTT CTTCTTCGTC AACATTTTTG TAGCCTTGAT AATCATCACA 
TTCCAGGAAC AGGGTGATAA GGTCTTGTCA GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC 
TGCATCGACT TTGCTATCAA TGCCAAACCT CTGACACGCT ACATGCCGCA GAACCAGCAG 
TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG GTCTCTCCGC CGTTTGAGTA CTCCATCATG 
ATCATGATTG CTCTCAACAC TGTGGTGCTC ATGATGAAGT TCCATGGGGC GCCTGACTTT 
TATGAGGCAA TGCTTAAGTA TCTCAACATT GTCTTCACTG TTCTCTTTTC ACTGGAGTGT 
ATTCTCAAGA TTATTGCTTT CGGCCCCTTG AATTACCTGA AAGATGCCTG GAATGTGTTT 
GACTTTGTGA CTGTGCTGGG CAGCATCACT GACATTTTAG TCACTGAGAT TAACACAACG 
GAGCGTCAGC TGAACTTCAG TTTCCTGAGG CTCTTTAGAG CAGCCCGACT CATCAAGCTC 
CTCCGGCAGG GATACACCAT TCGCATCCTG CTCTGGACAT TTGTCCAGTC TTTTAAGGCA 
CTGCCTTATG TTTGCCTTCT AATCGCCATG CTGTTTTTCA TCTACGCCAT TATTGGGATG 
CAGGTGTTTG GAAACATTGA TTTGAATGAT GACACGGCAA TCAACCGTCA CAACAACTTC 
CGCACTTTCC TCCAAGCTCT TATGCTTTTG TTCAGGAGTG CCACTGGCGA GGCTTGGCAT 
GATATCATGC TGTCGTGTCT GAGCGAGCGC ACCTGTGATC CGAGCTCAGG CACCCTTGGA 
AAGGAGTGTG GGAGTGACTT TGCCTACTTC TACTTTGTCT CCTTCATTTT CCTCTGCTCA 
TTTCTGATGT TGAACCTGTT TGTGGCCGTC ATTATGGACA ACTTTGAATA TCTGACACGC 
GACTCCTCCA TCCTGGGCCC TCACCACCTG GATGAGTTTA TTCGAGTGTG GGCGGAGTAT 
GACCCAGCCG CATGTGGCCG TATCAAGTAC CTCGATATGT ATGAGATGCT GCTCCACATG 
TCCCCACCTT TAGGTTTGGG AAAGAAATGT CCTCCACGAA TCGCCTACAA GCGTCTGGTG 
AAAATGAACA TGCCCATTGC GGATGACAAC AGTGTACACT TTACCTCAAC GCTGATGGCT 
CTGATCCGTA CTGCCCTGGA GATCAAACTG GCTTCAGGCA TGGTGGCTCA GCGTCTGTCA 
GATGCTGAAC TGAAGAAGGA GCTCTCTACA GTTTGGCCAA ACCTGTCTCA GAAGACCATG 
GACCTGCTGG TCACTCCACA TAAACCTAAT GAGCTCACAG TGGGGAAGGT GTATGCAGCT 
CTTATGATCT TTGACTATTA CAAGCAGAAC AGAGCAAAAA GACTTCAGAT GCAGCAACAG 
CAGCTGAAAG AACAGGCAGG ACCTGGATCT CAGAATAAAG TCAGCGCTCT CTTAGAGCCC 
ATTCTCCCAC TAACCCACAT GCAAGATCAG CCAGTGAACG GTGTCGATTC AGCTTCAGAG 
TGTCAAGCTC AGACCAGACC CAGCTCCACC ACACTCAACA ATGGACGCAC AACTCTTGAC 
AACACAATAA AAGGGTCTTC ATCCTGGGCC TCAGAGAAAT CCAAGGAAGT CCAAAAGTCT 
AAACGGAGAC CAATGTCTAG AGGTCAATCT GAGGATGCCT CAAATGCAAA CACTGCCCAG 
GAGTCAGTAG AAATGAGAAA GATGGAGAAC AGTGCCAACA CAGTGACCTC TGGTGGCCTT 
GAGGGCCAAG GACGTGCAGC GTCCATGCCA AAACTCAATG CTGAGATGCA GAGGAGTCAT 
TCCCGTCAAT CTCCTGGAAC ATTACTGGAA CCCATTCCAG ATTCCCCCAT GAGGCGCTCT 
GCATCCACTT TTGCACCACA GCGCCCTCAC GAGGTGAATC TGAATGACTA CATTCTGGAG 
AAACCTGTTC AGGAACGCCA TCACCACCAT CACCATCATC ACCGATGCCA TCATCGCCGG 
GACAGAGACA AGAAACAGAG ATCTCTGGAC AGGTCGCCTA CTGGCCATCA CAAAACTACA 
GGCACAGCCA CTGACTCTGT AGAGGAGAGG GCACATGACC GGGGTCGTTC CCATGAGAGG 
AAGCACCATT CATCTTCAGG AGAGAAGCAG CGGTATTACT CGTGTGATCG CTATGCCAGT 
CGGGAACACT GTTCCAGCAA ATCTGCCGTG GCAAGCTGTG CCACCTCACC CAGCGAGACC 
CAGGAGGCCA ATCTTAACAA GCAGGGCAGT GGGTGGGCTA AGGGGAGCCC GGTGTTGTTG 
ACTTCAGGGG CGAGCACTCC AAGCCGCGGG CGCAGACAGC TGCCCCAGAC CCCCCTCACT 
CCTCGTCCCT CCGTGGCCTA CAGGACTGCC AACTCTTCCC CTGTGCAACT TCTGGCCACA 
ACGACCAGCT TGCTTCCCCC GAGTCCCGGC AGGCTGAGCC GTGGTCTGTC CGAGCACAAC 
GCTCTTTTAC TCAGCGGCTC TATCTCCAGC TCGCCGGCCC CCGTCAGTCG CATTAACTCA 
GAACCCTTCT TGGGGCCCCC GGACCACCCT GCTGGCTTGC AGCCCGAAGC CTTCGCTGTC 
TTCCAAGAGA ATCCGAGCCC TCAGATGGGC CGTTCTCCTC AGACTGTAGG GATGCCCTTC 
GTGGCGATCC CCCCACCTCC CCCACAGCAG TTTCGCAGGG TCCCCAACGG GTACCACTTC 
TCCACGGGCC CCAGCGCCAG CAGAGGACGG GCACAGCAGT ACTACCCAGA GGCAGAGGTG 
GAGGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_017356097.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211586.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA.

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CloneID	ODa59087
Clone ID Related Accession (Same CDS sequence)	XM_017356097.1 , XM_017356097.2
Accession Version	XM_017356097.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC 
ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA 
CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC 
CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG 
GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG 
TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG 
TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG 
GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT 
CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC 
TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG 
GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT 
GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG 
AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT 
GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT 
CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC 
GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC 
AGCATCACTG ACATTTTAGT CACTGAGATT AACGAGCGTC AGCTGAACTT CAGTTTCCTG 
AGGCTCTTTA GAGCAGCCCG ACTCATCAAG CTCCTCCGGC AGGGATACAC CATTCGCATC 
CTGCTCTGGA CATTTGTCCA GTCTTTTAAG GCACTGCCTT ATGTTTGCCT TCTAATCGCC 
ATGCTGTTTT TCATCTACGC CATTATTGGG ATGCAGGTGT TTGGAAACAT TGATTTGAAT 
GATGACACGG CAATCAACCG TCACAACAAC TTCCGCACTT TCCTCCAAGC TCTTATGCTT 
TTGTTCAGGA GTGCCACTGG CGAGGCTTGG CATGATATCA TGCTGTCGTG TCTGAGCGAG 
CGCACCTGTG ATCCGAGCTC AGGCACCCTT GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC 
TTCTACTTTG TCTCCTTCAT TTTCCTCTGC TCATTTCTGA TGTTGAACCT GTTTGTGGCC 
GTCATTATGG ACAACTTTGA ATATCTGACA CGCGACTCCT CCATCCTGGG CCCTCACCAC 
CTGGATGAGT TTATTCGAGT GTGGGCGGAG TATGACCCAG CCGCATGTGG CCGTATCAAG 
TACCTCGATA TGTATGAGAT GCTGCTCCAC ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA 
TGTCCTCCAC GAATCGCCTA CAAGCGTCTG GTGAAAATGA ACATGCCCAT TGCGGATGAC 
AACAGTGTAC ACTTTACCTC AACGCTGATG GCTCTGATCC GTACTGCCCT GGAGATCAAA 
CTGGCTTCAG GCATGGTGGC TCAGCGTCTG TCAGATGCTG AACTGAAGAA GGAGCTCTCT 
ACAGTTTGGC CAAACCTGTC TCAGAAGACC ATGGACCTGC TGGTCACTCC ACATAAACCT 
AATGAGCTCA CAGTGGGGAA GGTGTATGCA GCTCTTATGA TCTTTGACTA TTACAAGCAG 
AACAGAGCAA AAAGACTTCA GATGCAGCAA CAGCAGCTGA AAGAACAGGC AGGACCTGGA 
TCTCAGAATA AAGTCAGCGC TCTCTTAGAG CCCATTCTCC CACTAACCCA CATGCAAGAT 
CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA GAGTGTCAAG CTCAGACCAG ACCCAGCTCC 
ACCACACTCA ACAATGGACG CACAACTCTT GACAACACAA TAAAAGGGTC TTCATCCTGG 
GCCTCAGAGA AATCCAAGGA AGTCCAAAAG TCTAAACGGA GACCAATGTC TAGAGGTCAA 
TCTGAGGATG CCTCAAATGC AAACACTGCC CAGGAGTCAG TAGAAATGAG AAAGATGGAG 
AACAGTGCCA ACACAGTGAC CTCTGGTGGC CTTGAGGGCC AAGGACGTGC AGCGTCCATG 
CCAAAACTCA ATGCTGAGAT GCAGAGGAGT CATTCCCGTC AATCTCCTGG AACATTACTG 
GAACCCATTC CAGATTCCCC CATGAGGCGC TCTGCATCCA CTTTTGCACC ACAGCGCCCT 
CACGAGGTGA ATCTGAATGA CTACATTCTG GAGAAACCTG TTCAGGAACG CCATCACCAC 
CATCACCATC ATCACCGATG CCATCATCGC CGGGACAGAG ACAAGAAACA GAGATCTCTG 
GACAGGTCGC CTACTGGCCA TCACAAAACT ACAGGCACAG CCACTGACTC TGTAGAGGAG 
AGGGCACATG ACCGGGGTCG TTCCCATGAG AGGAAGCACC ATTCATCTTC AGGAGAGAAG 
CAGCGGTATT ACTCGTGTGA TCGCTATGCC AGTCGGGAAC ACTGTTCCAG CAAATCTGCC 
GTGGCAAGCT GTGCCACCTC ACCCAGCGAG ACCCAGGAGG CCAATCTTAA CAAGCAGGGC 
AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG TTGACTTCAG GGGCGAGCAC TCCAAGCCGC 
GGGCGCAGAC AGCTGCCCCA GACCCCCCTC ACTCCTCGTC CCTCCGTGGC CTACAGGACT 
GCCAACTCTT CCCCTGTGCA ACTTCTGGCC ACAACGACCA GCTTGCTTCC CCCGAGTCCC 
GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC AACGCTCTTT TACTCAGCGG CTCTATCTCC 
AGCTCGCCGG CCCCCGTCAG TCGCATTAAC TCAGAACCCT TCTTGGGGCC CCCGGACCAC 
CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT GTCTTCCAAG AGAATCCGAG CCCTCAGATG 
GGCCGTTCTC CTCAGACTGT AGGGATGCCC TTCGTGGCGA TCCCCCCACC TCCCCCACAG 
CAGTTTCGCA GGGTCCCCAA CGGGTACCAC TTCTCCACGG GCCCCAGCGC CAGCAGAGGA 
CGGGCACAGC AGTACTACCC AGAGGCAGAG GTGGAGGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211586.1
CDS	636..7703
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_017356097.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356097.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC 
ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA 
CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC 
CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG 
GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG 
TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG 
TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG 
GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT 
CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC 
TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG 
GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT 
GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG 
AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT 
GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT 
CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC 
GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC 
AGCATCACTG ACATTTTAGT CACTGAGATT AACGAGCGTC AGCTGAACTT CAGTTTCCTG 
AGGCTCTTTA GAGCAGCCCG ACTCATCAAG CTCCTCCGGC AGGGATACAC CATTCGCATC 
CTGCTCTGGA CATTTGTCCA GTCTTTTAAG GCACTGCCTT ATGTTTGCCT TCTAATCGCC 
ATGCTGTTTT TCATCTACGC CATTATTGGG ATGCAGGTGT TTGGAAACAT TGATTTGAAT 
GATGACACGG CAATCAACCG TCACAACAAC TTCCGCACTT TCCTCCAAGC TCTTATGCTT 
TTGTTCAGGA GTGCCACTGG CGAGGCTTGG CATGATATCA TGCTGTCGTG TCTGAGCGAG 
CGCACCTGTG ATCCGAGCTC AGGCACCCTT GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC 
TTCTACTTTG TCTCCTTCAT TTTCCTCTGC TCATTTCTGA TGTTGAACCT GTTTGTGGCC 
GTCATTATGG ACAACTTTGA ATATCTGACA CGCGACTCCT CCATCCTGGG CCCTCACCAC 
CTGGATGAGT TTATTCGAGT GTGGGCGGAG TATGACCCAG CCGCATGTGG CCGTATCAAG 
TACCTCGATA TGTATGAGAT GCTGCTCCAC ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA 
TGTCCTCCAC GAATCGCCTA CAAGCGTCTG GTGAAAATGA ACATGCCCAT TGCGGATGAC 
AACAGTGTAC ACTTTACCTC AACGCTGATG GCTCTGATCC GTACTGCCCT GGAGATCAAA 
CTGGCTTCAG GCATGGTGGC TCAGCGTCTG TCAGATGCTG AACTGAAGAA GGAGCTCTCT 
ACAGTTTGGC CAAACCTGTC TCAGAAGACC ATGGACCTGC TGGTCACTCC ACATAAACCT 
AATGAGCTCA CAGTGGGGAA GGTGTATGCA GCTCTTATGA TCTTTGACTA TTACAAGCAG 
AACAGAGCAA AAAGACTTCA GATGCAGCAA CAGCAGCTGA AAGAACAGGC AGGACCTGGA 
TCTCAGAATA AAGTCAGCGC TCTCTTAGAG CCCATTCTCC CACTAACCCA CATGCAAGAT 
CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA GAGTGTCAAG CTCAGACCAG ACCCAGCTCC 
ACCACACTCA ACAATGGACG CACAACTCTT GACAACACAA TAAAAGGGTC TTCATCCTGG 
GCCTCAGAGA AATCCAAGGA AGTCCAAAAG TCTAAACGGA GACCAATGTC TAGAGGTCAA 
TCTGAGGATG CCTCAAATGC AAACACTGCC CAGGAGTCAG TAGAAATGAG AAAGATGGAG 
AACAGTGCCA ACACAGTGAC CTCTGGTGGC CTTGAGGGCC AAGGACGTGC AGCGTCCATG 
CCAAAACTCA ATGCTGAGAT GCAGAGGAGT CATTCCCGTC AATCTCCTGG AACATTACTG 
GAACCCATTC CAGATTCCCC CATGAGGCGC TCTGCATCCA CTTTTGCACC ACAGCGCCCT 
CACGAGGTGA ATCTGAATGA CTACATTCTG GAGAAACCTG TTCAGGAACG CCATCACCAC 
CATCACCATC ATCACCGATG CCATCATCGC CGGGACAGAG ACAAGAAACA GAGATCTCTG 
GACAGGTCGC CTACTGGCCA TCACAAAACT ACAGGCACAG CCACTGACTC TGTAGAGGAG 
AGGGCACATG ACCGGGGTCG TTCCCATGAG AGGAAGCACC ATTCATCTTC AGGAGAGAAG 
CAGCGGTATT ACTCGTGTGA TCGCTATGCC AGTCGGGAAC ACTGTTCCAG CAAATCTGCC 
GTGGCAAGCT GTGCCACCTC ACCCAGCGAG ACCCAGGAGG CCAATCTTAA CAAGCAGGGC 
AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG TTGACTTCAG GGGCGAGCAC TCCAAGCCGC 
GGGCGCAGAC AGCTGCCCCA GACCCCCCTC ACTCCTCGTC CCTCCGTGGC CTACAGGACT 
GCCAACTCTT CCCCTGTGCA ACTTCTGGCC ACAACGACCA GCTTGCTTCC CCCGAGTCCC 
GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC AACGCTCTTT TACTCAGCGG CTCTATCTCC 
AGCTCGCCGG CCCCCGTCAG TCGCATTAAC TCAGAACCCT TCTTGGGGCC CCCGGACCAC 
CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT GTCTTCCAAG AGAATCCGAG CCCTCAGATG 
GGCCGTTCTC CTCAGACTGT AGGGATGCCC TTCGTGGCGA TCCCCCCACC TCCCCCACAG 
CAGTTTCGCA GGGTCCCCAA CGGGTACCAC TTCTCCACGG GCCCCAGCGC CAGCAGAGGA 
CGGGCACAGC AGTACTACCC AGAGGCAGAG GTGGAGGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_017356097.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211586.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X18, mRNA.

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CloneID	ODa59087
Clone ID Related Accession (Same CDS sequence)	XM_017356097.1 , XM_017356097.2
Accession Version	XM_017356097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC 
ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA 
CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC 
CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG 
GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG 
TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG 
TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG 
GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT 
CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC 
TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG 
GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT 
GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG 
AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT 
GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT 
CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC 
GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC 
AGCATCACTG ACATTTTAGT CACTGAGATT AACGAGCGTC AGCTGAACTT CAGTTTCCTG 
AGGCTCTTTA GAGCAGCCCG ACTCATCAAG CTCCTCCGGC AGGGATACAC CATTCGCATC 
CTGCTCTGGA CATTTGTCCA GTCTTTTAAG GCACTGCCTT ATGTTTGCCT TCTAATCGCC 
ATGCTGTTTT TCATCTACGC CATTATTGGG ATGCAGGTGT TTGGAAACAT TGATTTGAAT 
GATGACACGG CAATCAACCG TCACAACAAC TTCCGCACTT TCCTCCAAGC TCTTATGCTT 
TTGTTCAGGA GTGCCACTGG CGAGGCTTGG CATGATATCA TGCTGTCGTG TCTGAGCGAG 
CGCACCTGTG ATCCGAGCTC AGGCACCCTT GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC 
TTCTACTTTG TCTCCTTCAT TTTCCTCTGC TCATTTCTGA TGTTGAACCT GTTTGTGGCC 
GTCATTATGG ACAACTTTGA ATATCTGACA CGCGACTCCT CCATCCTGGG CCCTCACCAC 
CTGGATGAGT TTATTCGAGT GTGGGCGGAG TATGACCCAG CCGCATGTGG CCGTATCAAG 
TACCTCGATA TGTATGAGAT GCTGCTCCAC ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA 
TGTCCTCCAC GAATCGCCTA CAAGCGTCTG GTGAAAATGA ACATGCCCAT TGCGGATGAC 
AACAGTGTAC ACTTTACCTC AACGCTGATG GCTCTGATCC GTACTGCCCT GGAGATCAAA 
CTGGCTTCAG GCATGGTGGC TCAGCGTCTG TCAGATGCTG AACTGAAGAA GGAGCTCTCT 
ACAGTTTGGC CAAACCTGTC TCAGAAGACC ATGGACCTGC TGGTCACTCC ACATAAACCT 
AATGAGCTCA CAGTGGGGAA GGTGTATGCA GCTCTTATGA TCTTTGACTA TTACAAGCAG 
AACAGAGCAA AAAGACTTCA GATGCAGCAA CAGCAGCTGA AAGAACAGGC AGGACCTGGA 
TCTCAGAATA AAGTCAGCGC TCTCTTAGAG CCCATTCTCC CACTAACCCA CATGCAAGAT 
CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA GAGTGTCAAG CTCAGACCAG ACCCAGCTCC 
ACCACACTCA ACAATGGACG CACAACTCTT GACAACACAA TAAAAGGGTC TTCATCCTGG 
GCCTCAGAGA AATCCAAGGA AGTCCAAAAG TCTAAACGGA GACCAATGTC TAGAGGTCAA 
TCTGAGGATG CCTCAAATGC AAACACTGCC CAGGAGTCAG TAGAAATGAG AAAGATGGAG 
AACAGTGCCA ACACAGTGAC CTCTGGTGGC CTTGAGGGCC AAGGACGTGC AGCGTCCATG 
CCAAAACTCA ATGCTGAGAT GCAGAGGAGT CATTCCCGTC AATCTCCTGG AACATTACTG 
GAACCCATTC CAGATTCCCC CATGAGGCGC TCTGCATCCA CTTTTGCACC ACAGCGCCCT 
CACGAGGTGA ATCTGAATGA CTACATTCTG GAGAAACCTG TTCAGGAACG CCATCACCAC 
CATCACCATC ATCACCGATG CCATCATCGC CGGGACAGAG ACAAGAAACA GAGATCTCTG 
GACAGGTCGC CTACTGGCCA TCACAAAACT ACAGGCACAG CCACTGACTC TGTAGAGGAG 
AGGGCACATG ACCGGGGTCG TTCCCATGAG AGGAAGCACC ATTCATCTTC AGGAGAGAAG 
CAGCGGTATT ACTCGTGTGA TCGCTATGCC AGTCGGGAAC ACTGTTCCAG CAAATCTGCC 
GTGGCAAGCT GTGCCACCTC ACCCAGCGAG ACCCAGGAGG CCAATCTTAA CAAGCAGGGC 
AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG TTGACTTCAG GGGCGAGCAC TCCAAGCCGC 
GGGCGCAGAC AGCTGCCCCA GACCCCCCTC ACTCCTCGTC CCTCCGTGGC CTACAGGACT 
GCCAACTCTT CCCCTGTGCA ACTTCTGGCC ACAACGACCA GCTTGCTTCC CCCGAGTCCC 
GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC AACGCTCTTT TACTCAGCGG CTCTATCTCC 
AGCTCGCCGG CCCCCGTCAG TCGCATTAAC TCAGAACCCT TCTTGGGGCC CCCGGACCAC 
CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT GTCTTCCAAG AGAATCCGAG CCCTCAGATG 
GGCCGTTCTC CTCAGACTGT AGGGATGCCC TTCGTGGCGA TCCCCCCACC TCCCCCACAG 
CAGTTTCGCA GGGTCCCCAA CGGGTACCAC TTCTCCACGG GCCCCAGCGC CAGCAGAGGA 
CGGGCACAGC AGTACTACCC AGAGGCAGAG GTGGAGGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211586.1
CDS	637..7704
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_017356097.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356097.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC 
ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA 
CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC 
CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG 
GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG 
TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG 
TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG 
GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT 
CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC 
TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG 
GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT 
GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG 
AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT 
GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT 
CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC 
GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC 
AGCATCACTG ACATTTTAGT CACTGAGATT AACGAGCGTC AGCTGAACTT CAGTTTCCTG 
AGGCTCTTTA GAGCAGCCCG ACTCATCAAG CTCCTCCGGC AGGGATACAC CATTCGCATC 
CTGCTCTGGA CATTTGTCCA GTCTTTTAAG GCACTGCCTT ATGTTTGCCT TCTAATCGCC 
ATGCTGTTTT TCATCTACGC CATTATTGGG ATGCAGGTGT TTGGAAACAT TGATTTGAAT 
GATGACACGG CAATCAACCG TCACAACAAC TTCCGCACTT TCCTCCAAGC TCTTATGCTT 
TTGTTCAGGA GTGCCACTGG CGAGGCTTGG CATGATATCA TGCTGTCGTG TCTGAGCGAG 
CGCACCTGTG ATCCGAGCTC AGGCACCCTT GGAAAGGAGT GTGGGAGTGA CTTTGCCTAC 
TTCTACTTTG TCTCCTTCAT TTTCCTCTGC TCATTTCTGA TGTTGAACCT GTTTGTGGCC 
GTCATTATGG ACAACTTTGA ATATCTGACA CGCGACTCCT CCATCCTGGG CCCTCACCAC 
CTGGATGAGT TTATTCGAGT GTGGGCGGAG TATGACCCAG CCGCATGTGG CCGTATCAAG 
TACCTCGATA TGTATGAGAT GCTGCTCCAC ATGTCCCCAC CTTTAGGTTT GGGAAAGAAA 
TGTCCTCCAC GAATCGCCTA CAAGCGTCTG GTGAAAATGA ACATGCCCAT TGCGGATGAC 
AACAGTGTAC ACTTTACCTC AACGCTGATG GCTCTGATCC GTACTGCCCT GGAGATCAAA 
CTGGCTTCAG GCATGGTGGC TCAGCGTCTG TCAGATGCTG AACTGAAGAA GGAGCTCTCT 
ACAGTTTGGC CAAACCTGTC TCAGAAGACC ATGGACCTGC TGGTCACTCC ACATAAACCT 
AATGAGCTCA CAGTGGGGAA GGTGTATGCA GCTCTTATGA TCTTTGACTA TTACAAGCAG 
AACAGAGCAA AAAGACTTCA GATGCAGCAA CAGCAGCTGA AAGAACAGGC AGGACCTGGA 
TCTCAGAATA AAGTCAGCGC TCTCTTAGAG CCCATTCTCC CACTAACCCA CATGCAAGAT 
CAGCCAGTGA ACGGTGTCGA TTCAGCTTCA GAGTGTCAAG CTCAGACCAG ACCCAGCTCC 
ACCACACTCA ACAATGGACG CACAACTCTT GACAACACAA TAAAAGGGTC TTCATCCTGG 
GCCTCAGAGA AATCCAAGGA AGTCCAAAAG TCTAAACGGA GACCAATGTC TAGAGGTCAA 
TCTGAGGATG CCTCAAATGC AAACACTGCC CAGGAGTCAG TAGAAATGAG AAAGATGGAG 
AACAGTGCCA ACACAGTGAC CTCTGGTGGC CTTGAGGGCC AAGGACGTGC AGCGTCCATG 
CCAAAACTCA ATGCTGAGAT GCAGAGGAGT CATTCCCGTC AATCTCCTGG AACATTACTG 
GAACCCATTC CAGATTCCCC CATGAGGCGC TCTGCATCCA CTTTTGCACC ACAGCGCCCT 
CACGAGGTGA ATCTGAATGA CTACATTCTG GAGAAACCTG TTCAGGAACG CCATCACCAC 
CATCACCATC ATCACCGATG CCATCATCGC CGGGACAGAG ACAAGAAACA GAGATCTCTG 
GACAGGTCGC CTACTGGCCA TCACAAAACT ACAGGCACAG CCACTGACTC TGTAGAGGAG 
AGGGCACATG ACCGGGGTCG TTCCCATGAG AGGAAGCACC ATTCATCTTC AGGAGAGAAG 
CAGCGGTATT ACTCGTGTGA TCGCTATGCC AGTCGGGAAC ACTGTTCCAG CAAATCTGCC 
GTGGCAAGCT GTGCCACCTC ACCCAGCGAG ACCCAGGAGG CCAATCTTAA CAAGCAGGGC 
AGTGGGTGGG CTAAGGGGAG CCCGGTGTTG TTGACTTCAG GGGCGAGCAC TCCAAGCCGC 
GGGCGCAGAC AGCTGCCCCA GACCCCCCTC ACTCCTCGTC CCTCCGTGGC CTACAGGACT 
GCCAACTCTT CCCCTGTGCA ACTTCTGGCC ACAACGACCA GCTTGCTTCC CCCGAGTCCC 
GGCAGGCTGA GCCGTGGTCT GTCCGAGCAC AACGCTCTTT TACTCAGCGG CTCTATCTCC 
AGCTCGCCGG CCCCCGTCAG TCGCATTAAC TCAGAACCCT TCTTGGGGCC CCCGGACCAC 
CCTGCTGGCT TGCAGCCCGA AGCCTTCGCT GTCTTCCAAG AGAATCCGAG CCCTCAGATG 
GGCCGTTCTC CTCAGACTGT AGGGATGCCC TTCGTGGCGA TCCCCCCACC TCCCCCACAG 
CAGTTTCGCA GGGTCCCCAA CGGGTACCAC TTCTCCACGG GCCCCAGCGC CAGCAGAGGA 
CGGGCACAGC AGTACTACCC AGAGGCAGAG GTGGAGGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( NM_001114548.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001108020.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), mRNA.

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CloneID	ODa36125
Clone ID Related Accession (Same CDS sequence)	NM_001114548.1
Accession Version	NM_001114548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929342.7 and BX571772.8. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGTCCA TTTGAGTACA TGATTCTCGC CACAATCATA 
GCAAACTGCA TTGTGTTGGG CTTAGAACAG CATTTACCAG CGTTAGACAA GACACCCATG 
TCAAAACGGC TGGATGACAC TGAGCCCTAC TTCATCGGAA TATTTTGCTT TGAGGCTGGG 
ATCAAGATCA TCGCTCTAGG TTTTGCTTTT CATAAAGGCT CCTACCTCCG CAATGGCTGG 
AATGTTATGG ATTTTGTTGT CGTGTTGACG GGAATTCTAA CCATAGTTGG GCCAGACTTT 
GATTTACGGA CACTCAGGGC AGTGAGAGTC TTGCGGCCAC TCAAACTCGT GTCTGGGATT 
CCTAGTCTCC AAGTAGTTCT GAAGTCCATC ATGAAAGCGA TGGTTCCTTT GCTTCAGATC 
GGCCTTCTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGTCTAGA CTTTTACATG 
GGCAAGTTTC ACCGCACCTG CTTCAGAACA GATACTGGGG AACAGGTGGA TGAATTTCCA 
TGTGGGCTCG AGTCGCCAGC CTGGACGTGT GAAAACGGCA CTGAATGTAG GGAATACTGG 
ATCGGCCCAA ACTTCGGCAT TACTAACTTT GACAACATCC TCTTCGCTGT GCTCACCGTC 
TTCCAGTGCA TCACTATGGA AGGATGGGTG GACATCCTTT ACAATGCCAA CGATGCCTCA 
GGGAACACCT GGAACTGGCT CTACTTCATC CCACTCATCA TCATCGGCTC CTTCTTCATG 
CTCAACCTGG TTCTGGGTGT GCTGTCAGGG GAGTTTGCAA AGGAGCGAGA GCGTGTGGAG 
AAAAGACAGG AGTTCTTAAA GCTGCGCAGA CAACAGCAGA TCGAAAGAGA GTTAACCGGA 
TACCTGGAGT GGATCTGCAA GGCAGAGGAA GTAATGCTTG CTGAAGAAGA TAAAAATGCA 
GAGGACAAGG ATGATGTTGC CTGGTACAAG AGGAAATGCA ACAACCCAGT TTTGAAGCGG 
GCCAAGAAGA GCAAAAATGA CCTCATCAAT GCAGAGGAAG GAGAAGATCA CTTTACTGAC 
ATCTCTTCAG TTGCTCCCCA AGGCTCTCCA TTCACCCGCA CAAGTGTGAA GAGCAGCAAG 
ATTGAGAGCT TGTCGTACTT TCGCAGAAAA GAAAAGAGGT TTCGCTTCTT CATCCGCCGC 
ATGGTGAAGG CTCAGAGTTT CTACTGGACT GTTCTGTGTA TAGTGGGACT GAACACACTG 
TGTGTGGCCA TAGTCCACTA CGACCAGCCC GAATGGCTCA CATATGCACT GTATTTGGCG 
GAGTTTGTGT TCCTTGGTCT GTTCCTAATA GAGATGAGTC TGAAGATGTA TGGCCTCGGG 
CCCAGGACTT ACTTTCACTC TTCATTTAAC TGCTTTGACT TTGGGGTGAT TGTGGGGAGC 
ATTTTCGAGG TGATCTGGGC TGCAGTAAAG CCAGGAGCTT CCTTTGGCAT TAGTGTGTTG 
CGAGCTCTTC GTTTGCTAAG AATATTCAAG GTTACCAAGT ATTGGAACTC ATTGAGGAAT 
CTGGTAGTGT CTCTGCTCAA CTCAATGAAG TCCATCATTA GTCTCCTGTT TCTGCTGTTC 
CTCTTCATCG TGGTGTTTGC TCTGTTGGGA ATGCAGCTCT TTGGGGGACA GTTTAATTTT 
GAAGACGAGA CCCCGACAAC CAACTTTGAC ACATTCCCAG CTGCTATCTT GACAGTGTTT 
CAGATTTTGA CTGGAGAGGA CTGGAACGCT GTGATGTATC ATGGGATTGA ATCGCAGGGC 
GGAGTGCACC GGGGAATGTT CTGCTCCGTG TACTTCATCG TGCTCACGCT GTTTGGAAAC 
TACACTCTCC TCAATGTCTT TTTGGCCATC GCTGTTGATA ACCTTGCCAA TGCACAAGAA 
CTCACCAAGG ATGAGGAGGA GCAAGAGGAG GCTATCAGTA AGAAACTGGC TCTACAGAAA 
GCCAAGGAGG TAAAGGAGGT CAGCCCCATG TCGGCAGCTA ACATTTCCAT CACAGCTTTT 
ATAAGGCCAA ACCCTGGTGC TCTTTCATGC AGCTCCTCCA ACTCCAGCTC TCATTCACCT 
AAAGAACAGC AGCGTTCTCT AAAAATGATG TCCGTGTGGG AGCAGCGCAC CACTCAGATC 
CGAAAGCACA TGCTAGCCAG CAGTGAGGCG TTGTACCAAG AAGAGCTCAA TCCACGGCTC 
ATGTCTAACC TTCCTCTACG CACCGACATA AAAACCCACC TGGACCGGCC TTTGGTTGTG 
GAGCCTCGCT GTGATGGCCC CATCAGCCCA CACAGTGGCT GGGACAAACC CCAGGATTTG 
CAAGGCGAGG GCAGCACACC AGAAGAACGA GTCCCACCTG TATTCGAGCC TCCACAGTCA 
CATCCACCAC GCAAGCACCA CCGTCACAGA GAGCGTCTTT CTAATGAGAC CAATGAAAAT 
GGAGACACGG GTCATGCCAA AGAAGGCCGG CATCACGTTC ATCACAGCCG AAGTAAAGAG 
CATGATGGGA CACGTTGTAA GGAGGGGAAG GGTGACAGGA GCCGAAGCAG AGAAGGGGGA 
CGTAGACATC ACCATCAGAG CTCGGTGGAT GAGGGCGTAG GAGGTGGAGA GAGGGAACAT 
CGGCACCATC ACTCCCATAG GCATAACAGA GAGGGCAATG GTGTGGTCAG TGCTGGGGGG 
AAGAGTGAAC GGCGGTCCAG GCAGAAAGAG GGCTCACGAT CAGGGACAAG AGAGGGAGAG 
AGATGCTCAA GGGGGGAGAA TGGAGGAAAT GGAGGGAGGA GGCGACACAG GCCACGAACC 
AGTAAAGCTC AGTCTACGCT GGAAGGAGAG GAGCAACATG TGAATGGAGA GGAGGAGGGC 
AAGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGCTCTTCAA GGGCAAGTTT 
TTTTACTGCA CAGACGAGTC CAAGGGCCTG GAGAAAGACT GCAGGGGGCT GTTTTTGGAT 
TATGATAAGG ATGATGTGAC GGCACAACCG AGGGAATGGA AGAAATACGA GTTTCACTAT 
GACAATGTGC TTTGGGCTTT TCTCACTCTC TTCACTGTTT CTACGGGAGA GGGCTGGCCT 
ACGGTTTTGA AGCACTCCGT GGATGCCACT TTTGAGGATC AGGGTCCCAG TCCAGGCTAC 
CGCATTGAGA TGTCTATTTT TTACGTGGTC TACTTTGTGG TTTTCCCTTT CTTCTTCGTC 
AACATTTTTG TAGCCTTGAT AATCATCACA TTCCAGGAAC AGGGTGATAA GGTCTTGTCA 
GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC TGCATCGACT TTGCTATCAA TGCCAAACCT 
CTGACACGCT ACATGCCGCA GAACCAGCAG TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG 
GTCTCTCCGC CGTTTGAGTA CTCCATCATG ATCATGATTG CTCTCAACAC TGTGGTGCTC 
ATGATGAAGT TCCATGGGGC GCCTGACTTT TATGAGGCAA TGCTTAAGTA TCTCAACATT 
GTCTTCACTG TTCTCTTTTC ACTGGAGTGT ATTCTCAAGA TTATTGCTTT CGGCCCCTTG 
AATTACCTGA AAGATGCCTG GAATGTGTTT GACTTTGTGA CTGTGCTGGG CAGCATCACT 
GACATTTTAG TCACTGAGAT TAACGAGCGT CAGCTGAACT TCAGTTTCCT GAGGCTCTTT 
AGAGCAGCCC GACTCATCAA GCTCCTCCGG CAGGGATACA CCATTCGCAT CCTGCTCTGG 
ACATTTGTCC AGTCTTTTAA GGCACTGCCT TATGTTTGCC TTCTAATCGC CATGCTGTTT 
TTCATCTACG CCATTATTGG GATGCAGGTG TTTGGAAACA TTGATTTGAA TGATGACACG 
GCAATCAACC GTCACAACAA CTTCCGCACT TTCCTCCAAG CTCTTATGCT TTTGTTCAGG 
AGTGCCACTG GCGAGGCTTG GCATGATATC ATGCTGTCGT GTCTGAGCGA GCGCACCTGT 
GATCCGAGCT CAGGCACCCT TGGAAAGGAG TGTGGGAGTG ACTTTGCCTA CTTCTACTTT 
GTCTCCTTCA TTTTCCTCTG CTCATTTCTG ATGTTGAACC TGTTTGTGGC CGTCATTATG 
GACAACTTTG AATATCTGAC ACGCGACTCC TCCATCCTGG GCCCTCACCA CCTGGATGAG 
TTTATTCGAG TGTGGGCGGA GTATGACCCA GCCGCATGTG GCCGTATCAA GTACCTCGAT 
ATGTATGAGA TGCTGCTCCA CATGTCCCCA CCTTTAGGTT TGGGAAAGAA ATGTCCTCCA 
CGAATCGCCT ACAAGCGTCT GGTGAAAATG AACATGCCCA TTGCGGATGA CAACAGTGTA 
CACTTTACCT CAACGCTGAT GGCTCTGATC CGTACTGCCC TGGAGATCAA ACTGGCTTCA 
GGCATGGTGG CTCAGCGTCT GTCAGATGCT GAACTGAAGA AGGAGCTCTC TACAGTTTGG 
CCAAACCTGT CTCAGAAGAC CATGGACCTG CTGGTCACTC CACATAAACC TAATGAGCTC 
ACAGTGGGGA AGGTGTATGC AGCTCTTATG ATCTTTGACT ATTACAAGCA GAACAGAGCA 
AAAAGACTTC AGATGCAGCA ACAGCAGCTG AAAGAACAGG CAGGACCTGG ATCTCAGAAT 
AAAGTCAGCG CTCTCTTAGA GCCCATTCTC CCACTAACCC ACATGCAAGA TCAGCCAGTG 
AACGGTGTCG ATTCAGCTTC AGAGTGTCAA GCTCAGACCA GACCCAGCTC CACCACACTC 
AACAATGGAC GCACAACTCT TGACAACACA ATAAAAGGGT CTTCATCCTG GGCCTCAGAG 
AAATCCAAGG AAGTCCAAAA GTCTAAACGG AGACCAATGT CTAGAGGTCA ATCTGAGGAT 
GCCTCAAATG CAAACACTGC CCAGGAGTCA GTAGAAATGA GAAAGATGGA GAACAGTGCC 
AACACAGTGA CCTCTGGTGG CCTTGAGGGC CAAGGACGTG CAGCGTCCAT GCCAAAACTC 
AATGCTGAGA TGCAGAGGAG TCATTCCCGT CAATCTCCTG GAACATTACT GGAACCCATT 
CCAGATTCCC CCATGAGGCG CTCTGCATCC ACTTTTGCAC CACAGCGCCC TCACGAGGTG 
AATCTGAATG ACTACATTCT GGAGAAACCT GTTCAGGAAC GCCATCACCA CCATCACCAT 
CATCACCGAT GCCATCATCG CCGGGACAGA GACAAGAAAC AGAGATCTCT GGACAGGTCG 
CCTACTGGCC ATCACAAAAC TACAGGCACA GCCACTGACT CTGTAGAGGA GAGGGCACAT 
GACCGGGGTC GTTCCCATGA GAGGAAGCAC CATTCATCTT CAGGAGAGAA GCAGCGGTAT 
TACTCGTGTG ATCGCTATGC CAGTCGGGAA CACTGTTCCA GCAAATCTGC CGTGGCAAGC 
TGTGCCACCT CACCCAGCGA GACCCAGGAG GCCAATCTTA ACAAGCAGGG CAGTGGGTGG 
GCTAAGGGGA GCCCGGTGTT GTTGACTTCA GGGGCGAGCA CTCCAAGCCG CGGGCGCAGA 
CAGCTGCCCC AGACCCCCCT CACTCCTCGT CCCTCCGTGG CCTACAGGAC TGCCAACTCT 
TCCCCTGTGC AACTTCTGGC CACAACGACC AGCTTGCTTC CCCCGAGTCC CGGCAGGCTG 
AGCCGTGGTC TGTCCGAGCA CAACGCTCTT TTACTCAGCG GCTCTATCTC CAGCTCGCCG 
GCCCCCGTCA GTCGCATTAA CTCAGAACCC TTCTTGGGGC CCCCGGACCA CCCTGCTGGC 
TTGCAGCCCG AAGCCTTCGC TGTCTTCCAA GAGAATCCGA GCCCTCAGAT GGGCCGTTCT 
CCTCAGACTG TAGGGATGCC CTTCGTGGCG ATCCCCCCAC CTCCCCCACA GCAGTTTCGC 
AGGGTCCCCA ACGGGTACCA CTTCTCCACG GGCCCCAGCG CCAGCAGAGG ACGGGCACAG 
CAGTACTACC CAGAGGCAGA GGTGGAGGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108020.1
CDS	1..6939
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITECPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	NM_001114548.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114548.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGTCCA TTTGAGTACA TGATTCTCGC CACAATCATA 
GCAAACTGCA TTGTGTTGGG CTTAGAACAG CATTTACCAG CGTTAGACAA GACACCCATG 
TCAAAACGGC TGGATGACAC TGAGCCCTAC TTCATCGGAA TATTTTGCTT TGAGGCTGGG 
ATCAAGATCA TCGCTCTAGG TTTTGCTTTT CATAAAGGCT CCTACCTCCG CAATGGCTGG 
AATGTTATGG ATTTTGTTGT CGTGTTGACG GGAATTCTAA CCATAGTTGG GCCAGACTTT 
GATTTACGGA CACTCAGGGC AGTGAGAGTC TTGCGGCCAC TCAAACTCGT GTCTGGGATT 
CCTAGTCTCC AAGTAGTTCT GAAGTCCATC ATGAAAGCGA TGGTTCCTTT GCTTCAGATC 
GGCCTTCTGC TCTTCTTTGC CATCCTTATG TTTGCTATCA TCGGTCTAGA CTTTTACATG 
GGCAAGTTTC ACCGCACCTG CTTCAGAACA GATACTGGGG AACAGGTGGA TGAATTTCCA 
TGTGGGCTCG AGTCGCCAGC CTGGACGTGT GAAAACGGCA CTGAATGTAG GGAATACTGG 
ATCGGCCCAA ACTTCGGCAT TACTAACTTT GACAACATCC TCTTCGCTGT GCTCACCGTC 
TTCCAGTGCA TCACTATGGA AGGATGGGTG GACATCCTTT ACAATGCCAA CGATGCCTCA 
GGGAACACCT GGAACTGGCT CTACTTCATC CCACTCATCA TCATCGGCTC CTTCTTCATG 
CTCAACCTGG TTCTGGGTGT GCTGTCAGGG GAGTTTGCAA AGGAGCGAGA GCGTGTGGAG 
AAAAGACAGG AGTTCTTAAA GCTGCGCAGA CAACAGCAGA TCGAAAGAGA GTTAACCGGA 
TACCTGGAGT GGATCTGCAA GGCAGAGGAA GTAATGCTTG CTGAAGAAGA TAAAAATGCA 
GAGGACAAGG ATGATGTTGC CTGGTACAAG AGGAAATGCA ACAACCCAGT TTTGAAGCGG 
GCCAAGAAGA GCAAAAATGA CCTCATCAAT GCAGAGGAAG GAGAAGATCA CTTTACTGAC 
ATCTCTTCAG TTGCTCCCCA AGGCTCTCCA TTCACCCGCA CAAGTGTGAA GAGCAGCAAG 
ATTGAGAGCT TGTCGTACTT TCGCAGAAAA GAAAAGAGGT TTCGCTTCTT CATCCGCCGC 
ATGGTGAAGG CTCAGAGTTT CTACTGGACT GTTCTGTGTA TAGTGGGACT GAACACACTG 
TGTGTGGCCA TAGTCCACTA CGACCAGCCC GAATGGCTCA CATATGCACT GTATTTGGCG 
GAGTTTGTGT TCCTTGGTCT GTTCCTAATA GAGATGAGTC TGAAGATGTA TGGCCTCGGG 
CCCAGGACTT ACTTTCACTC TTCATTTAAC TGCTTTGACT TTGGGGTGAT TGTGGGGAGC 
ATTTTCGAGG TGATCTGGGC TGCAGTAAAG CCAGGAGCTT CCTTTGGCAT TAGTGTGTTG 
CGAGCTCTTC GTTTGCTAAG AATATTCAAG GTTACCAAGT ATTGGAACTC ATTGAGGAAT 
CTGGTAGTGT CTCTGCTCAA CTCAATGAAG TCCATCATTA GTCTCCTGTT TCTGCTGTTC 
CTCTTCATCG TGGTGTTTGC TCTGTTGGGA ATGCAGCTCT TTGGGGGACA GTTTAATTTT 
GAAGACGAGA CCCCGACAAC CAACTTTGAC ACATTCCCAG CTGCTATCTT GACAGTGTTT 
CAGATTTTGA CTGGAGAGGA CTGGAACGCT GTGATGTATC ATGGGATTGA ATCGCAGGGC 
GGAGTGCACC GGGGAATGTT CTGCTCCGTG TACTTCATCG TGCTCACGCT GTTTGGAAAC 
TACACTCTCC TCAATGTCTT TTTGGCCATC GCTGTTGATA ACCTTGCCAA TGCACAAGAA 
CTCACCAAGG ATGAGGAGGA GCAAGAGGAG GCTATCAGTA AGAAACTGGC TCTACAGAAA 
GCCAAGGAGG TAAAGGAGGT CAGCCCCATG TCGGCAGCTA ACATTTCCAT CACAGCTTTT 
ATAAGGCCAA ACCCTGGTGC TCTTTCATGC AGCTCCTCCA ACTCCAGCTC TCATTCACCT 
AAAGAACAGC AGCGTTCTCT AAAAATGATG TCCGTGTGGG AGCAGCGCAC CACTCAGATC 
CGAAAGCACA TGCTAGCCAG CAGTGAGGCG TTGTACCAAG AAGAGCTCAA TCCACGGCTC 
ATGTCTAACC TTCCTCTACG CACCGACATA AAAACCCACC TGGACCGGCC TTTGGTTGTG 
GAGCCTCGCT GTGATGGCCC CATCAGCCCA CACAGTGGCT GGGACAAACC CCAGGATTTG 
CAAGGCGAGG GCAGCACACC AGAAGAACGA GTCCCACCTG TATTCGAGCC TCCACAGTCA 
CATCCACCAC GCAAGCACCA CCGTCACAGA GAGCGTCTTT CTAATGAGAC CAATGAAAAT 
GGAGACACGG GTCATGCCAA AGAAGGCCGG CATCACGTTC ATCACAGCCG AAGTAAAGAG 
CATGATGGGA CACGTTGTAA GGAGGGGAAG GGTGACAGGA GCCGAAGCAG AGAAGGGGGA 
CGTAGACATC ACCATCAGAG CTCGGTGGAT GAGGGCGTAG GAGGTGGAGA GAGGGAACAT 
CGGCACCATC ACTCCCATAG GCATAACAGA GAGGGCAATG GTGTGGTCAG TGCTGGGGGG 
AAGAGTGAAC GGCGGTCCAG GCAGAAAGAG GGCTCACGAT CAGGGACAAG AGAGGGAGAG 
AGATGCTCAA GGGGGGAGAA TGGAGGAAAT GGAGGGAGGA GGCGACACAG GCCACGAACC 
AGTAAAGCTC AGTCTACGCT GGAAGGAGAG GAGCAACATG TGAATGGAGA GGAGGAGGGC 
AAGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGCTCTTCAA GGGCAAGTTT 
TTTTACTGCA CAGACGAGTC CAAGGGCCTG GAGAAAGACT GCAGGGGGCT GTTTTTGGAT 
TATGATAAGG ATGATGTGAC GGCACAACCG AGGGAATGGA AGAAATACGA GTTTCACTAT 
GACAATGTGC TTTGGGCTTT TCTCACTCTC TTCACTGTTT CTACGGGAGA GGGCTGGCCT 
ACGGTTTTGA AGCACTCCGT GGATGCCACT TTTGAGGATC AGGGTCCCAG TCCAGGCTAC 
CGCATTGAGA TGTCTATTTT TTACGTGGTC TACTTTGTGG TTTTCCCTTT CTTCTTCGTC 
AACATTTTTG TAGCCTTGAT AATCATCACA TTCCAGGAAC AGGGTGATAA GGTCTTGTCA 
GAGTGCAGTC TGGAGAAGAA TGAGAGAGCC TGCATCGACT TTGCTATCAA TGCCAAACCT 
CTGACACGCT ACATGCCGCA GAACCAGCAG TCCTTTCAGT ACCGCCTGTG GAAGTTTGTG 
GTCTCTCCGC CGTTTGAGTA CTCCATCATG ATCATGATTG CTCTCAACAC TGTGGTGCTC 
ATGATGAAGT TCCATGGGGC GCCTGACTTT TATGAGGCAA TGCTTAAGTA TCTCAACATT 
GTCTTCACTG TTCTCTTTTC ACTGGAGTGT ATTCTCAAGA TTATTGCTTT CGGCCCCTTG 
AATTACCTGA AAGATGCCTG GAATGTGTTT GACTTTGTGA CTGTGCTGGG CAGCATCACT 
GACATTTTAG TCACTGAGAT TAACGAGCGT CAGCTGAACT TCAGTTTCCT GAGGCTCTTT 
AGAGCAGCCC GACTCATCAA GCTCCTCCGG CAGGGATACA CCATTCGCAT CCTGCTCTGG 
ACATTTGTCC AGTCTTTTAA GGCACTGCCT TATGTTTGCC TTCTAATCGC CATGCTGTTT 
TTCATCTACG CCATTATTGG GATGCAGGTG TTTGGAAACA TTGATTTGAA TGATGACACG 
GCAATCAACC GTCACAACAA CTTCCGCACT TTCCTCCAAG CTCTTATGCT TTTGTTCAGG 
AGTGCCACTG GCGAGGCTTG GCATGATATC ATGCTGTCGT GTCTGAGCGA GCGCACCTGT 
GATCCGAGCT CAGGCACCCT TGGAAAGGAG TGTGGGAGTG ACTTTGCCTA CTTCTACTTT 
GTCTCCTTCA TTTTCCTCTG CTCATTTCTG ATGTTGAACC TGTTTGTGGC CGTCATTATG 
GACAACTTTG AATATCTGAC ACGCGACTCC TCCATCCTGG GCCCTCACCA CCTGGATGAG 
TTTATTCGAG TGTGGGCGGA GTATGACCCA GCCGCATGTG GCCGTATCAA GTACCTCGAT 
ATGTATGAGA TGCTGCTCCA CATGTCCCCA CCTTTAGGTT TGGGAAAGAA ATGTCCTCCA 
CGAATCGCCT ACAAGCGTCT GGTGAAAATG AACATGCCCA TTGCGGATGA CAACAGTGTA 
CACTTTACCT CAACGCTGAT GGCTCTGATC CGTACTGCCC TGGAGATCAA ACTGGCTTCA 
GGCATGGTGG CTCAGCGTCT GTCAGATGCT GAACTGAAGA AGGAGCTCTC TACAGTTTGG 
CCAAACCTGT CTCAGAAGAC CATGGACCTG CTGGTCACTC CACATAAACC TAATGAGCTC 
ACAGTGGGGA AGGTGTATGC AGCTCTTATG ATCTTTGACT ATTACAAGCA GAACAGAGCA 
AAAAGACTTC AGATGCAGCA ACAGCAGCTG AAAGAACAGG CAGGACCTGG ATCTCAGAAT 
AAAGTCAGCG CTCTCTTAGA GCCCATTCTC CCACTAACCC ACATGCAAGA TCAGCCAGTG 
AACGGTGTCG ATTCAGCTTC AGAGTGTCAA GCTCAGACCA GACCCAGCTC CACCACACTC 
AACAATGGAC GCACAACTCT TGACAACACA ATAAAAGGGT CTTCATCCTG GGCCTCAGAG 
AAATCCAAGG AAGTCCAAAA GTCTAAACGG AGACCAATGT CTAGAGGTCA ATCTGAGGAT 
GCCTCAAATG CAAACACTGC CCAGGAGTCA GTAGAAATGA GAAAGATGGA GAACAGTGCC 
AACACAGTGA CCTCTGGTGG CCTTGAGGGC CAAGGACGTG CAGCGTCCAT GCCAAAACTC 
AATGCTGAGA TGCAGAGGAG TCATTCCCGT CAATCTCCTG GAACATTACT GGAACCCATT 
CCAGATTCCC CCATGAGGCG CTCTGCATCC ACTTTTGCAC CACAGCGCCC TCACGAGGTG 
AATCTGAATG ACTACATTCT GGAGAAACCT GTTCAGGAAC GCCATCACCA CCATCACCAT 
CATCACCGAT GCCATCATCG CCGGGACAGA GACAAGAAAC AGAGATCTCT GGACAGGTCG 
CCTACTGGCC ATCACAAAAC TACAGGCACA GCCACTGACT CTGTAGAGGA GAGGGCACAT 
GACCGGGGTC GTTCCCATGA GAGGAAGCAC CATTCATCTT CAGGAGAGAA GCAGCGGTAT 
TACTCGTGTG ATCGCTATGC CAGTCGGGAA CACTGTTCCA GCAAATCTGC CGTGGCAAGC 
TGTGCCACCT CACCCAGCGA GACCCAGGAG GCCAATCTTA ACAAGCAGGG CAGTGGGTGG 
GCTAAGGGGA GCCCGGTGTT GTTGACTTCA GGGGCGAGCA CTCCAAGCCG CGGGCGCAGA 
CAGCTGCCCC AGACCCCCCT CACTCCTCGT CCCTCCGTGG CCTACAGGAC TGCCAACTCT 
TCCCCTGTGC AACTTCTGGC CACAACGACC AGCTTGCTTC CCCCGAGTCC CGGCAGGCTG 
AGCCGTGGTC TGTCCGAGCA CAACGCTCTT TTACTCAGCG GCTCTATCTC CAGCTCGCCG 
GCCCCCGTCA GTCGCATTAA CTCAGAACCC TTCTTGGGGC CCCCGGACCA CCCTGCTGGC 
TTGCAGCCCG AAGCCTTCGC TGTCTTCCAA GAGAATCCGA GCCCTCAGAT GGGCCGTTCT 
CCTCAGACTG TAGGGATGCC CTTCGTGGCG ATCCCCCCAC CTCCCCCACA GCAGTTTCGC 
AGGGTCCCCA ACGGGTACCA CTTCTCCACG GGCCCCAGCG CCAGCAGAGG ACGGGCACAG 
CAGTACTACC CAGAGGCAGA GGTGGAGGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301492.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299767.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA.

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CloneID	ODa42256
Clone ID Related Accession (Same CDS sequence)	XM_009301492.2
Accession Version	XM_009301492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT 
CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG 
TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT 
GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC 
ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA 
GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT 
GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT 
TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG 
CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG 
TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA 
GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG 
GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC 
ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG 
TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC 
CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT 
CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA 
GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC 
ACTGAGATTA ACACAACGGA GCGTCAGCTG AACTTCAGTT TCCTGAGGCT CTTTAGAGCA 
GCCCGACTCA TCAAGCTCCT CCGGCAGGGA TACACCATTC GCATCCTGCT CTGGACATTT 
GTCCAGTCTT TTAAGGCACT GCCTTATGTT TGCCTTCTAA TCGCCATGCT GTTTTTCATC 
TACGCCATTA TTGGGATGCA GGTGTTTGGA AACATTGATT TGAATGATGA CACGGCAATC 
AACCGTCACA ACAACTTCCG CACTTTCCTC CAAGCTCTTA TGCTTTTGTT CAGGAGTGCC 
ACTGGCGAGG CTTGGCATGA TATCATGCTG TCGTGTCTGA GCGAGCGCAC CTGTGATCCG 
AGCTCAGGCA CCCTTGGAAA GGAGTGTGGG AGTGACTTTG CCTACTTCTA CTTTGTCTCC 
TTCATTTTCC TCTGCTCATT TCTGATGTTG AACCTGTTTG TGGCCGTCAT TATGGACAAC 
TTTGAATATC TGACACGCGA CTCCTCCATC CTGGGCCCTC ACCACCTGGA TGAGTTTATT 
CGAGTGTGGG CGGAGTATGA CCCAGCCGCA TGTGGCCGTA TCAAGTACCT CGATATGTAT 
GAGATGCTGC TCCACATGTC CCCACCTTTA GGTTTGGGAA AGAAATGTCC TCCACGAATC 
GCCTACAAGC GTCTGGTGAA AATGAACATG CCCATTGCGG ATGACAACAG TGTACACTTT 
ACCTCAACGC TGATGGCTCT GATCCGTACT GCCCTGGAGA TCAAACTGGC TTCAGGCATG 
GTGGCTCAGC GTCTGTCAGA TGCTGAACTG AAGAAGGAGC TCTCTACAGT TTGGCCAAAC 
CTGTCTCAGA AGACCATGGA CCTGCTGGTC ACTCCACATA AACCTAATGA GCTCACAGTG 
GGGAAGGTGT ATGCAGCTCT TATGATCTTT GACTATTACA AGCAGAACAG AGCAAAAAGA 
CTTCAGATGC AGCAACAGCA GCTGAAAGAA CAGGCAGGAC CTGGATCTCA GAATAAAGTC 
AGCGCTCTCT TAGAGCCCAT TCTCCCACTA ACCCACATGC AAGATCAGCC AGTGAACGGT 
GTCGATTCAG CTTCAGAGTG TCAAGCTCAG ACCAGACCCA GCTCCACCAC ACTCAACAAT 
GGACGCACAA CTCTTGACAA CACAATAAAA GGGTCTTCAT CCTGGGCCTC AGAGAAATCC 
AAGGAAGTCC AAAAGTCTAA ACGGAGACCA ATGTCTAGAG GTCAATCTGA GGATGCCTCA 
AATGCAAACA CTGCCCAGGA GTCAGTAGAA ATGAGAAAGA TGGAGAACAG TGCCAACACA 
GTGACCTCTG GTGGCCTTGA GGGCCAAGGA CGTGCAGCGT CCATGCCAAA ACTCAATGCT 
GAGATGCAGA GGAGTCATTC CCGTCAATCT CCTGGAACAT TACTGGAACC CATTCCAGAT 
TCCCCCATGA GGCGCTCTGC ATCCACTTTT GCACCACAGC GCCCTCACGA GGTGAATCTG 
AATGACTACA TTCTGGAGAA ACCTGTTCAG GAACGCCATC ACCACCATCA CCATCATCAC 
CGATGCCATC ATCGCCGGGA CAGAGACAAG AAACAGAGAT CTCTGGACAG GTCGCCTACT 
GGCCATCACA AAACTACAGG CACAGCCACT GACTCTGTAG AGGAGAGGGC ACATGACCGG 
GGTCGTTCCC ATGAGAGGAA GCACCATTCA TCTTCAGGAG AGAAGCAGCG GTATTACTCG 
TGTGATCGCT ATGCCAGTCG GGAACACTGT TCCAGCAAAT CTGCCGTGGC AAGCTGTGCC 
ACCTCACCCA GCGAGACCCA GGAGGCCAAT CTTAACAAGC AGGGCAGTGG GTGGGCTAAG 
GGGAGCCCGG TGTTGTTGAC TTCAGGGGCG AGCACTCCAA GCCGCGGGCG CAGACAGCTG 
CCCCAGACCC CCCTCACTCC TCGTCCCTCC GTGGCCTACA GGACTGCCAA CTCTTCCCCT 
GTGCAACTTC TGGCCACAAC GACCAGCTTG CTTCCCCCGA GTCCCGGCAG GCTGAGCCGT 
GGTCTGTCCG AGCACAACGC TCTTTTACTC AGCGGCTCTA TCTCCAGCTC GCCGGCCCCC 
GTCAGTCGCA TTAACTCAGA ACCCTTCTTG GGGCCCCCGG ACCACCCTGC TGGCTTGCAG 
CCCGAAGCCT TCGCTGTCTT CCAAGAGAAT CCGAGCCCTC AGATGGGCCG TTCTCCTCAG 
ACTGTAGGGA TGCCCTTCGT GGCGATCCCC CCACCTCCCC CACAGCAGTT TCGCAGGGTC 
CCCAACGGGT ACCACTTCTC CACGGGCCCC AGCGCCAGCA GAGGACGGGC ACAGCAGTAC 
TACCCAGAGG CAGAGGTGGA GGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299767.1
CDS	636..7808
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPSYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301492.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301492.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT 
CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG 
TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT 
GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC 
ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA 
GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT 
GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT 
TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG 
CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG 
TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA 
GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG 
GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC 
ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG 
TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC 
CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT 
CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA 
GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC 
ACTGAGATTA ACACAACGGA GCGTCAGCTG AACTTCAGTT TCCTGAGGCT CTTTAGAGCA 
GCCCGACTCA TCAAGCTCCT CCGGCAGGGA TACACCATTC GCATCCTGCT CTGGACATTT 
GTCCAGTCTT TTAAGGCACT GCCTTATGTT TGCCTTCTAA TCGCCATGCT GTTTTTCATC 
TACGCCATTA TTGGGATGCA GGTGTTTGGA AACATTGATT TGAATGATGA CACGGCAATC 
AACCGTCACA ACAACTTCCG CACTTTCCTC CAAGCTCTTA TGCTTTTGTT CAGGAGTGCC 
ACTGGCGAGG CTTGGCATGA TATCATGCTG TCGTGTCTGA GCGAGCGCAC CTGTGATCCG 
AGCTCAGGCA CCCTTGGAAA GGAGTGTGGG AGTGACTTTG CCTACTTCTA CTTTGTCTCC 
TTCATTTTCC TCTGCTCATT TCTGATGTTG AACCTGTTTG TGGCCGTCAT TATGGACAAC 
TTTGAATATC TGACACGCGA CTCCTCCATC CTGGGCCCTC ACCACCTGGA TGAGTTTATT 
CGAGTGTGGG CGGAGTATGA CCCAGCCGCA TGTGGCCGTA TCAAGTACCT CGATATGTAT 
GAGATGCTGC TCCACATGTC CCCACCTTTA GGTTTGGGAA AGAAATGTCC TCCACGAATC 
GCCTACAAGC GTCTGGTGAA AATGAACATG CCCATTGCGG ATGACAACAG TGTACACTTT 
ACCTCAACGC TGATGGCTCT GATCCGTACT GCCCTGGAGA TCAAACTGGC TTCAGGCATG 
GTGGCTCAGC GTCTGTCAGA TGCTGAACTG AAGAAGGAGC TCTCTACAGT TTGGCCAAAC 
CTGTCTCAGA AGACCATGGA CCTGCTGGTC ACTCCACATA AACCTAATGA GCTCACAGTG 
GGGAAGGTGT ATGCAGCTCT TATGATCTTT GACTATTACA AGCAGAACAG AGCAAAAAGA 
CTTCAGATGC AGCAACAGCA GCTGAAAGAA CAGGCAGGAC CTGGATCTCA GAATAAAGTC 
AGCGCTCTCT TAGAGCCCAT TCTCCCACTA ACCCACATGC AAGATCAGCC AGTGAACGGT 
GTCGATTCAG CTTCAGAGTG TCAAGCTCAG ACCAGACCCA GCTCCACCAC ACTCAACAAT 
GGACGCACAA CTCTTGACAA CACAATAAAA GGGTCTTCAT CCTGGGCCTC AGAGAAATCC 
AAGGAAGTCC AAAAGTCTAA ACGGAGACCA ATGTCTAGAG GTCAATCTGA GGATGCCTCA 
AATGCAAACA CTGCCCAGGA GTCAGTAGAA ATGAGAAAGA TGGAGAACAG TGCCAACACA 
GTGACCTCTG GTGGCCTTGA GGGCCAAGGA CGTGCAGCGT CCATGCCAAA ACTCAATGCT 
GAGATGCAGA GGAGTCATTC CCGTCAATCT CCTGGAACAT TACTGGAACC CATTCCAGAT 
TCCCCCATGA GGCGCTCTGC ATCCACTTTT GCACCACAGC GCCCTCACGA GGTGAATCTG 
AATGACTACA TTCTGGAGAA ACCTGTTCAG GAACGCCATC ACCACCATCA CCATCATCAC 
CGATGCCATC ATCGCCGGGA CAGAGACAAG AAACAGAGAT CTCTGGACAG GTCGCCTACT 
GGCCATCACA AAACTACAGG CACAGCCACT GACTCTGTAG AGGAGAGGGC ACATGACCGG 
GGTCGTTCCC ATGAGAGGAA GCACCATTCA TCTTCAGGAG AGAAGCAGCG GTATTACTCG 
TGTGATCGCT ATGCCAGTCG GGAACACTGT TCCAGCAAAT CTGCCGTGGC AAGCTGTGCC 
ACCTCACCCA GCGAGACCCA GGAGGCCAAT CTTAACAAGC AGGGCAGTGG GTGGGCTAAG 
GGGAGCCCGG TGTTGTTGAC TTCAGGGGCG AGCACTCCAA GCCGCGGGCG CAGACAGCTG 
CCCCAGACCC CCCTCACTCC TCGTCCCTCC GTGGCCTACA GGACTGCCAA CTCTTCCCCT 
GTGCAACTTC TGGCCACAAC GACCAGCTTG CTTCCCCCGA GTCCCGGCAG GCTGAGCCGT 
GGTCTGTCCG AGCACAACGC TCTTTTACTC AGCGGCTCTA TCTCCAGCTC GCCGGCCCCC 
GTCAGTCGCA TTAACTCAGA ACCCTTCTTG GGGCCCCCGG ACCACCCTGC TGGCTTGCAG 
CCCGAAGCCT TCGCTGTCTT CCAAGAGAAT CCGAGCCCTC AGATGGGCCG TTCTCCTCAG 
ACTGTAGGGA TGCCCTTCGT GGCGATCCCC CCACCTCCCC CACAGCAGTT TCGCAGGGTC 
CCCAACGGGT ACCACTTCTC CACGGGCCCC AGCGCCAGCA GAGGACGGGC ACAGCAGTAC 
TACCCAGAGG CAGAGGTGGA GGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301493.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299768.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA.

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CloneID	ODa42257
Clone ID Related Accession (Same CDS sequence)	XM_009301493.2
Accession Version	XM_009301493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT 
CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG 
TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT 
GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC 
ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA 
GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT 
GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT 
TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG 
CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG 
TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA 
GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG 
GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC 
ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG 
TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC 
CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT 
CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA 
GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC 
ACTGAGATTA ACGAGCGTCA GCTGAACTTC AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA 
CTCATCAAGC TCCTCCGGCA GGGATACACC ATTCGCATCC TGCTCTGGAC ATTTGTCCAG 
TCTTTTAAGG CACTGCCTTA TGTTTGCCTT CTAATCGCCA TGCTGTTTTT CATCTACGCC 
ATTATTGGGA TGCAGGTGTT TGGAAACATT GATTTGAATG ATGACACGGC AATCAACCGT 
CACAACAACT TCCGCACTTT CCTCCAAGCT CTTATGCTTT TGTTCAGGAG TGCCACTGGC 
GAGGCTTGGC ATGATATCAT GCTGTCGTGT CTGAGCGAGC GCACCTGTGA TCCGAGCTCA 
GGCACCCTTG GAAAGGAGTG TGGGAGTGAC TTTGCCTACT TCTACTTTGT CTCCTTCATT 
TTCCTCTGCT CATTTCTGAT GTTGAACCTG TTTGTGGCCG TCATTATGGA CAACTTTGAA 
TATCTGACAC GCGACTCCTC CATCCTGGGC CCTCACCACC TGGATGAGTT TATTCGAGTG 
TGGGCGGAGT ATGACCCAGC CGCATGTGGC CGTATCAAGT ACCTCGATAT GTATGAGATG 
CTGCTCCACA TGTCCCCACC TTTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC 
AAGCGTCTGG TGAAAATGAA CATGCCCATT GCGGATGACA ACAGTGTACA CTTTACCTCA 
ACGCTGATGG CTCTGATCCG TACTGCCCTG GAGATCAAAC TGGCTTCAGG CATGGTGGCT 
CAGCGTCTGT CAGATGCTGA ACTGAAGAAG GAGCTCTCTA CAGTTTGGCC AAACCTGTCT 
CAGAAGACCA TGGACCTGCT GGTCACTCCA CATAAACCTA ATGAGCTCAC AGTGGGGAAG 
GTGTATGCAG CTCTTATGAT CTTTGACTAT TACAAGCAGA ACAGAGCAAA AAGACTTCAG 
ATGCAGCAAC AGCAGCTGAA AGAACAGGCA GGACCTGGAT CTCAGAATAA AGTCAGCGCT 
CTCTTAGAGC CCATTCTCCC ACTAACCCAC ATGCAAGATC AGCCAGTGAA CGGTGTCGAT 
TCAGCTTCAG AGTGTCAAGC TCAGACCAGA CCCAGCTCCA CCACACTCAA CAATGGACGC 
ACAACTCTTG ACAACACAAT AAAAGGGTCT TCATCCTGGG CCTCAGAGAA ATCCAAGGAA 
GTCCAAAAGT CTAAACGGAG ACCAATGTCT AGAGGTCAAT CTGAGGATGC CTCAAATGCA 
AACACTGCCC AGGAGTCAGT AGAAATGAGA AAGATGGAGA ACAGTGCCAA CACAGTGACC 
TCTGGTGGCC TTGAGGGCCA AGGACGTGCA GCGTCCATGC CAAAACTCAA TGCTGAGATG 
CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA ACATTACTGG AACCCATTCC AGATTCCCCC 
ATGAGGCGCT CTGCATCCAC TTTTGCACCA CAGCGCCCTC ACGAGGTGAA TCTGAATGAC 
TACATTCTGG AGAAACCTGT TCAGGAACGC CATCACCACC ATCACCATCA TCACCGATGC 
CATCATCGCC GGGACAGAGA CAAGAAACAG AGATCTCTGG ACAGGTCGCC TACTGGCCAT 
CACAAAACTA CAGGCACAGC CACTGACTCT GTAGAGGAGA GGGCACATGA CCGGGGTCGT 
TCCCATGAGA GGAAGCACCA TTCATCTTCA GGAGAGAAGC AGCGGTATTA CTCGTGTGAT 
CGCTATGCCA GTCGGGAACA CTGTTCCAGC AAATCTGCCG TGGCAAGCTG TGCCACCTCA 
CCCAGCGAGA CCCAGGAGGC CAATCTTAAC AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC 
CCGGTGTTGT TGACTTCAGG GGCGAGCACT CCAAGCCGCG GGCGCAGACA GCTGCCCCAG 
ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC TACAGGACTG CCAACTCTTC CCCTGTGCAA 
CTTCTGGCCA CAACGACCAG CTTGCTTCCC CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG 
TCCGAGCACA ACGCTCTTTT ACTCAGCGGC TCTATCTCCA GCTCGCCGGC CCCCGTCAGT 
CGCATTAACT CAGAACCCTT CTTGGGGCCC CCGGACCACC CTGCTGGCTT GCAGCCCGAA 
GCCTTCGCTG TCTTCCAAGA GAATCCGAGC CCTCAGATGG GCCGTTCTCC TCAGACTGTA 
GGGATGCCCT TCGTGGCGAT CCCCCCACCT CCCCCACAGC AGTTTCGCAG GGTCCCCAAC 
GGGTACCACT TCTCCACGGG CCCCAGCGCC AGCAGAGGAC GGGCACAGCA GTACTACCCA 
GAGGCAGAGG TGGAGGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299768.1
CDS	637..7803
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPSYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301493.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301493.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT 
CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG 
TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT 
GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC 
ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA 
GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT 
GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT 
TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG 
CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG 
TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA 
GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG 
GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC 
ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG 
TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC 
CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT 
CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA 
GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC 
ACTGAGATTA ACGAGCGTCA GCTGAACTTC AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA 
CTCATCAAGC TCCTCCGGCA GGGATACACC ATTCGCATCC TGCTCTGGAC ATTTGTCCAG 
TCTTTTAAGG CACTGCCTTA TGTTTGCCTT CTAATCGCCA TGCTGTTTTT CATCTACGCC 
ATTATTGGGA TGCAGGTGTT TGGAAACATT GATTTGAATG ATGACACGGC AATCAACCGT 
CACAACAACT TCCGCACTTT CCTCCAAGCT CTTATGCTTT TGTTCAGGAG TGCCACTGGC 
GAGGCTTGGC ATGATATCAT GCTGTCGTGT CTGAGCGAGC GCACCTGTGA TCCGAGCTCA 
GGCACCCTTG GAAAGGAGTG TGGGAGTGAC TTTGCCTACT TCTACTTTGT CTCCTTCATT 
TTCCTCTGCT CATTTCTGAT GTTGAACCTG TTTGTGGCCG TCATTATGGA CAACTTTGAA 
TATCTGACAC GCGACTCCTC CATCCTGGGC CCTCACCACC TGGATGAGTT TATTCGAGTG 
TGGGCGGAGT ATGACCCAGC CGCATGTGGC CGTATCAAGT ACCTCGATAT GTATGAGATG 
CTGCTCCACA TGTCCCCACC TTTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC 
AAGCGTCTGG TGAAAATGAA CATGCCCATT GCGGATGACA ACAGTGTACA CTTTACCTCA 
ACGCTGATGG CTCTGATCCG TACTGCCCTG GAGATCAAAC TGGCTTCAGG CATGGTGGCT 
CAGCGTCTGT CAGATGCTGA ACTGAAGAAG GAGCTCTCTA CAGTTTGGCC AAACCTGTCT 
CAGAAGACCA TGGACCTGCT GGTCACTCCA CATAAACCTA ATGAGCTCAC AGTGGGGAAG 
GTGTATGCAG CTCTTATGAT CTTTGACTAT TACAAGCAGA ACAGAGCAAA AAGACTTCAG 
ATGCAGCAAC AGCAGCTGAA AGAACAGGCA GGACCTGGAT CTCAGAATAA AGTCAGCGCT 
CTCTTAGAGC CCATTCTCCC ACTAACCCAC ATGCAAGATC AGCCAGTGAA CGGTGTCGAT 
TCAGCTTCAG AGTGTCAAGC TCAGACCAGA CCCAGCTCCA CCACACTCAA CAATGGACGC 
ACAACTCTTG ACAACACAAT AAAAGGGTCT TCATCCTGGG CCTCAGAGAA ATCCAAGGAA 
GTCCAAAAGT CTAAACGGAG ACCAATGTCT AGAGGTCAAT CTGAGGATGC CTCAAATGCA 
AACACTGCCC AGGAGTCAGT AGAAATGAGA AAGATGGAGA ACAGTGCCAA CACAGTGACC 
TCTGGTGGCC TTGAGGGCCA AGGACGTGCA GCGTCCATGC CAAAACTCAA TGCTGAGATG 
CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA ACATTACTGG AACCCATTCC AGATTCCCCC 
ATGAGGCGCT CTGCATCCAC TTTTGCACCA CAGCGCCCTC ACGAGGTGAA TCTGAATGAC 
TACATTCTGG AGAAACCTGT TCAGGAACGC CATCACCACC ATCACCATCA TCACCGATGC 
CATCATCGCC GGGACAGAGA CAAGAAACAG AGATCTCTGG ACAGGTCGCC TACTGGCCAT 
CACAAAACTA CAGGCACAGC CACTGACTCT GTAGAGGAGA GGGCACATGA CCGGGGTCGT 
TCCCATGAGA GGAAGCACCA TTCATCTTCA GGAGAGAAGC AGCGGTATTA CTCGTGTGAT 
CGCTATGCCA GTCGGGAACA CTGTTCCAGC AAATCTGCCG TGGCAAGCTG TGCCACCTCA 
CCCAGCGAGA CCCAGGAGGC CAATCTTAAC AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC 
CCGGTGTTGT TGACTTCAGG GGCGAGCACT CCAAGCCGCG GGCGCAGACA GCTGCCCCAG 
ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC TACAGGACTG CCAACTCTTC CCCTGTGCAA 
CTTCTGGCCA CAACGACCAG CTTGCTTCCC CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG 
TCCGAGCACA ACGCTCTTTT ACTCAGCGGC TCTATCTCCA GCTCGCCGGC CCCCGTCAGT 
CGCATTAACT CAGAACCCTT CTTGGGGCCC CCGGACCACC CTGCTGGCTT GCAGCCCGAA 
GCCTTCGCTG TCTTCCAAGA GAATCCGAGC CCTCAGATGG GCCGTTCTCC TCAGACTGTA 
GGGATGCCCT TCGTGGCGAT CCCCCCACCT CCCCCACAGC AGTTTCGCAG GGTCCCCAAC 
GGGTACCACT TCTCCACGGG CCCCAGCGCC AGCAGAGGAC GGGCACAGCA GTACTACCCA 
GAGGCAGAGG TGGAGGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301494.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299769.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA.

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CloneID	ODa42258
Clone ID Related Accession (Same CDS sequence)	XM_009301494.2
Accession Version	XM_009301494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CATCACATCA AAGCGTAAAC 
AGAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG 
CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG 
AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC 
GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG 
GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA 
CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC 
ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT 
GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC 
GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC 
ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG 
AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC 
CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC 
ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT 
GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG 
GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT 
GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC 
ACAACGGAGC GTCAGCTGAA CTTCAGTTTC CTGAGGCTCT TTAGAGCAGC CCGACTCATC 
AAGCTCCTCC GGCAGGGATA CACCATTCGC ATCCTGCTCT GGACATTTGT CCAGTCTTTT 
AAGGCACTGC CTTATGTTTG CCTTCTAATC GCCATGCTGT TTTTCATCTA CGCCATTATT 
GGGATGCAGG TGTTTGGAAA CATTGATTTG AATGATGACA CGGCAATCAA CCGTCACAAC 
AACTTCCGCA CTTTCCTCCA AGCTCTTATG CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT 
TGGCATGATA TCATGCTGTC GTGTCTGAGC GAGCGCACCT GTGATCCGAG CTCAGGCACC 
CTTGGAAAGG AGTGTGGGAG TGACTTTGCC TACTTCTACT TTGTCTCCTT CATTTTCCTC 
TGCTCATTTC TGATGTTGAA CCTGTTTGTG GCCGTCATTA TGGACAACTT TGAATATCTG 
ACACGCGACT CCTCCATCCT GGGCCCTCAC CACCTGGATG AGTTTATTCG AGTGTGGGCG 
GAGTATGACC CAGCCGCATG TGGCCGTATC AAGTACCTCG ATATGTATGA GATGCTGCTC 
CACATGTCCC CACCTTTAGG TTTGGGAAAG AAATGTCCTC CACGAATCGC CTACAAGCGT 
CTGGTGAAAA TGAACATGCC CATTGCGGAT GACAACAGTG TACACTTTAC CTCAACGCTG 
ATGGCTCTGA TCCGTACTGC CCTGGAGATC AAACTGGCTT CAGGCATGGT GGCTCAGCGT 
CTGTCAGATG CTGAACTGAA GAAGGAGCTC TCTACAGTTT GGCCAAACCT GTCTCAGAAG 
ACCATGGACC TGCTGGTCAC TCCACATAAA CCTAATGAGC TCACAGTGGG GAAGGTGTAT 
GCAGCTCTTA TGATCTTTGA CTATTACAAG CAGAACAGAG CAAAAAGACT TCAGATGCAG 
CAACAGCAGC TGAAAGAACA GGCAGGACCT GGATCTCAGA ATAAAGTCAG CGCTCTCTTA 
GAGCCCATTC TCCCACTAAC CCACATGCAA GATCAGCCAG TGAACGGTGT CGATTCAGCT 
TCAGAGTGTC AAGCTCAGAC CAGACCCAGC TCCACCACAC TCAACAATGG ACGCACAACT 
CTTGACAACA CAATAAAAGG GTCTTCATCC TGGGCCTCAG AGAAATCCAA GGAAGTCCAA 
AAGTCTAAAC GGAGACCAAT GTCTAGAGGT CAATCTGAGG ATGCCTCAAA TGCAAACACT 
GCCCAGGAGT CAGTAGAAAT GAGAAAGATG GAGAACAGTG CCAACACAGT GACCTCTGGT 
GGCCTTGAGG GCCAAGGACG TGCAGCGTCC ATGCCAAAAC TCAATGCTGA GATGCAGAGG 
AGTCATTCCC GTCAATCTCC TGGAACATTA CTGGAACCCA TTCCAGATTC CCCCATGAGG 
CGCTCTGCAT CCACTTTTGC ACCACAGCGC CCTCACGAGG TGAATCTGAA TGACTACATT 
CTGGAGAAAC CTGTTCAGGA ACGCCATCAC CACCATCACC ATCATCACCG ATGCCATCAT 
CGCCGGGACA GAGACAAGAA ACAGAGATCT CTGGACAGGT CGCCTACTGG CCATCACAAA 
ACTACAGGCA CAGCCACTGA CTCTGTAGAG GAGAGGGCAC ATGACCGGGG TCGTTCCCAT 
GAGAGGAAGC ACCATTCATC TTCAGGAGAG AAGCAGCGGT ATTACTCGTG TGATCGCTAT 
GCCAGTCGGG AACACTGTTC CAGCAAATCT GCCGTGGCAA GCTGTGCCAC CTCACCCAGC 
GAGACCCAGG AGGCCAATCT TAACAAGCAG GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG 
TTGTTGACTT CAGGGGCGAG CACTCCAAGC CGCGGGCGCA GACAGCTGCC CCAGACCCCC 
CTCACTCCTC GTCCCTCCGT GGCCTACAGG ACTGCCAACT CTTCCCCTGT GCAACTTCTG 
GCCACAACGA CCAGCTTGCT TCCCCCGAGT CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG 
CACAACGCTC TTTTACTCAG CGGCTCTATC TCCAGCTCGC CGGCCCCCGT CAGTCGCATT 
AACTCAGAAC CCTTCTTGGG GCCCCCGGAC CACCCTGCTG GCTTGCAGCC CGAAGCCTTC 
GCTGTCTTCC AAGAGAATCC GAGCCCTCAG ATGGGCCGTT CTCCTCAGAC TGTAGGGATG 
CCCTTCGTGG CGATCCCCCC ACCTCCCCCA CAGCAGTTTC GCAGGGTCCC CAACGGGTAC 
CACTTCTCCA CGGGCCCCAG CGCCAGCAGA GGACGGGCAC AGCAGTACTA CCCAGAGGCA 
GAGGTGGAGG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299769.1
CDS	636..7796
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPSYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301494.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301494.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CATCACATCA AAGCGTAAAC 
AGAGGAACCA AGGGCAAAGA CATCAGCACT ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG 
CGACCTCTGA AGACCATCAA ACGTCTACCA AAACTGAAGG CCGTGTTTGA TTGTGTGGTG 
AACTCCCTGA AGAATGTCCT GAATATCCTC ATTGTCTACA TTCTGTTCAT GTTCATTTTC 
GCTGTCATTG CTGTGCAGCT CTTCAAGGGC AAGTTTTTTT ACTGCACAGA CGAGTCCAAG 
GGCCTGGAGA AAGACTGCAG GGGGCTGTTT TTGGATTATG ATAAGGATGA TGTGACGGCA 
CAACCGAGGG AATGGAAGAA ATACGAGTTT CACTATGACA ATGTGCTTTG GGCTTTTCTC 
ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC TGGCCTACGG TTTTGAAGCA CTCCGTGGAT 
GCCACTTTTG AGGATCAGGG TCCCAGTCCA GGCTACCGCA TTGAGATGTC TATTTTTTAC 
GTGGTCTACT TTGTGGTTTT CCCTTTCTTC TTCGTCAACA TTTTTGTAGC CTTGATAATC 
ATCACATTCC AGGAACAGGG TGATAAGGTC TTGTCAGAGT GCAGTCTGGA GAAGAATGAG 
AGAGCCTGCA TCGACTTTGC TATCAATGCC AAACCTCTGA CACGCTACAT GCCGCAGAAC 
CAGCAGTCCT TTCAGTACCG CCTGTGGAAG TTTGTGGTCT CTCCGCCGTT TGAGTACTCC 
ATCATGATCA TGATTGCTCT CAACACTGTG GTGCTCATGA TGAAGTTCCA TGGGGCGCCT 
GACTTTTATG AGGCAATGCT TAAGTATCTC AACATTGTCT TCACTGTTCT CTTTTCACTG 
GAGTGTATTC TCAAGATTAT TGCTTTCGGC CCCTTGAATT ACCTGAAAGA TGCCTGGAAT 
GTGTTTGACT TTGTGACTGT GCTGGGCAGC ATCACTGACA TTTTAGTCAC TGAGATTAAC 
ACAACGGAGC GTCAGCTGAA CTTCAGTTTC CTGAGGCTCT TTAGAGCAGC CCGACTCATC 
AAGCTCCTCC GGCAGGGATA CACCATTCGC ATCCTGCTCT GGACATTTGT CCAGTCTTTT 
AAGGCACTGC CTTATGTTTG CCTTCTAATC GCCATGCTGT TTTTCATCTA CGCCATTATT 
GGGATGCAGG TGTTTGGAAA CATTGATTTG AATGATGACA CGGCAATCAA CCGTCACAAC 
AACTTCCGCA CTTTCCTCCA AGCTCTTATG CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT 
TGGCATGATA TCATGCTGTC GTGTCTGAGC GAGCGCACCT GTGATCCGAG CTCAGGCACC 
CTTGGAAAGG AGTGTGGGAG TGACTTTGCC TACTTCTACT TTGTCTCCTT CATTTTCCTC 
TGCTCATTTC TGATGTTGAA CCTGTTTGTG GCCGTCATTA TGGACAACTT TGAATATCTG 
ACACGCGACT CCTCCATCCT GGGCCCTCAC CACCTGGATG AGTTTATTCG AGTGTGGGCG 
GAGTATGACC CAGCCGCATG TGGCCGTATC AAGTACCTCG ATATGTATGA GATGCTGCTC 
CACATGTCCC CACCTTTAGG TTTGGGAAAG AAATGTCCTC CACGAATCGC CTACAAGCGT 
CTGGTGAAAA TGAACATGCC CATTGCGGAT GACAACAGTG TACACTTTAC CTCAACGCTG 
ATGGCTCTGA TCCGTACTGC CCTGGAGATC AAACTGGCTT CAGGCATGGT GGCTCAGCGT 
CTGTCAGATG CTGAACTGAA GAAGGAGCTC TCTACAGTTT GGCCAAACCT GTCTCAGAAG 
ACCATGGACC TGCTGGTCAC TCCACATAAA CCTAATGAGC TCACAGTGGG GAAGGTGTAT 
GCAGCTCTTA TGATCTTTGA CTATTACAAG CAGAACAGAG CAAAAAGACT TCAGATGCAG 
CAACAGCAGC TGAAAGAACA GGCAGGACCT GGATCTCAGA ATAAAGTCAG CGCTCTCTTA 
GAGCCCATTC TCCCACTAAC CCACATGCAA GATCAGCCAG TGAACGGTGT CGATTCAGCT 
TCAGAGTGTC AAGCTCAGAC CAGACCCAGC TCCACCACAC TCAACAATGG ACGCACAACT 
CTTGACAACA CAATAAAAGG GTCTTCATCC TGGGCCTCAG AGAAATCCAA GGAAGTCCAA 
AAGTCTAAAC GGAGACCAAT GTCTAGAGGT CAATCTGAGG ATGCCTCAAA TGCAAACACT 
GCCCAGGAGT CAGTAGAAAT GAGAAAGATG GAGAACAGTG CCAACACAGT GACCTCTGGT 
GGCCTTGAGG GCCAAGGACG TGCAGCGTCC ATGCCAAAAC TCAATGCTGA GATGCAGAGG 
AGTCATTCCC GTCAATCTCC TGGAACATTA CTGGAACCCA TTCCAGATTC CCCCATGAGG 
CGCTCTGCAT CCACTTTTGC ACCACAGCGC CCTCACGAGG TGAATCTGAA TGACTACATT 
CTGGAGAAAC CTGTTCAGGA ACGCCATCAC CACCATCACC ATCATCACCG ATGCCATCAT 
CGCCGGGACA GAGACAAGAA ACAGAGATCT CTGGACAGGT CGCCTACTGG CCATCACAAA 
ACTACAGGCA CAGCCACTGA CTCTGTAGAG GAGAGGGCAC ATGACCGGGG TCGTTCCCAT 
GAGAGGAAGC ACCATTCATC TTCAGGAGAG AAGCAGCGGT ATTACTCGTG TGATCGCTAT 
GCCAGTCGGG AACACTGTTC CAGCAAATCT GCCGTGGCAA GCTGTGCCAC CTCACCCAGC 
GAGACCCAGG AGGCCAATCT TAACAAGCAG GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG 
TTGTTGACTT CAGGGGCGAG CACTCCAAGC CGCGGGCGCA GACAGCTGCC CCAGACCCCC 
CTCACTCCTC GTCCCTCCGT GGCCTACAGG ACTGCCAACT CTTCCCCTGT GCAACTTCTG 
GCCACAACGA CCAGCTTGCT TCCCCCGAGT CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG 
CACAACGCTC TTTTACTCAG CGGCTCTATC TCCAGCTCGC CGGCCCCCGT CAGTCGCATT 
AACTCAGAAC CCTTCTTGGG GCCCCCGGAC CACCCTGCTG GCTTGCAGCC CGAAGCCTTC 
GCTGTCTTCC AAGAGAATCC GAGCCCTCAG ATGGGCCGTT CTCCTCAGAC TGTAGGGATG 
CCCTTCGTGG CGATCCCCCC ACCTCCCCCA CAGCAGTTTC GCAGGGTCCC CAACGGGTAC 
CACTTCTCCA CGGGCCCCAG CGCCAGCAGA GGACGGGCAC AGCAGTACTA CCCAGAGGCA 
GAGGTGGAGG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301495.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299770.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA.

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CloneID	ODa42259
Clone ID Related Accession (Same CDS sequence)	XM_009301495.2
Accession Version	XM_009301495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGAGGAACC 
AAGGGCAAAG ACATCAGCAC TATCAAGTCA CTGAGGGTGT TGCGAGTCTT GCGACCTCTG 
AAGACCATCA AACGTCTACC AAAACTGAAG GCCGTGTTTG ATTGTGTGGT GAACTCCCTG 
AAGAATGTCC TGAATATCCT CATTGTCTAC ATTCTGTTCA TGTTCATTTT CGCTGTCATT 
GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT TACTGCACAG ACGAGTCCAA GGGCCTGGAG 
AAAGACTGCA GGGGGCTGTT TTTGGATTAT GATAAGGATG ATGTGACGGC ACAACCGAGG 
GAATGGAAGA AATACGAGTT TCACTATGAC AATGTGCTTT GGGCTTTTCT CACTCTCTTC 
ACTGTTTCTA CGGGAGAGGG CTGGCCTACG GTTTTGAAGC ACTCCGTGGA TGCCACTTTT 
GAGGATCAGG GTCCCAGTCC AGGCTACCGC ATTGAGATGT CTATTTTTTA CGTGGTCTAC 
TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC ATTTTTGTAG CCTTGATAAT CATCACATTC 
CAGGAACAGG GTGATAAGGT CTTGTCAGAG TGCAGTCTGG AGAAGAATGA GAGAGCCTGC 
ATCGACTTTG CTATCAATGC CAAACCTCTG ACACGCTACA TGCCGCAGAA CCAGCAGTCC 
TTTCAGTACC GCCTGTGGAA GTTTGTGGTC TCTCCGCCGT TTGAGTACTC CATCATGATC 
ATGATTGCTC TCAACACTGT GGTGCTCATG ATGAAGTTCC ATGGGGCGCC TGACTTTTAT 
GAGGCAATGC TTAAGTATCT CAACATTGTC TTCACTGTTC TCTTTTCACT GGAGTGTATT 
CTCAAGATTA TTGCTTTCGG CCCCTTGAAT TACCTGAAAG ATGCCTGGAA TGTGTTTGAC 
TTTGTGACTG TGCTGGGCAG CATCACTGAC ATTTTAGTCA CTGAGATTAA CACAACGGAG 
CGTCAGCTGA ACTTCAGTTT CCTGAGGCTC TTTAGAGCAG CCCGACTCAT CAAGCTCCTC 
CGGCAGGGAT ACACCATTCG CATCCTGCTC TGGACATTTG TCCAGTCTTT TAAGGCACTG 
CCTTATGTTT GCCTTCTAAT CGCCATGCTG TTTTTCATCT ACGCCATTAT TGGGATGCAG 
GTGTTTGGAA ACATTGATTT GAATGATGAC ACGGCAATCA ACCGTCACAA CAACTTCCGC 
ACTTTCCTCC AAGCTCTTAT GCTTTTGTTC AGGAGTGCCA CTGGCGAGGC TTGGCATGAT 
ATCATGCTGT CGTGTCTGAG CGAGCGCACC TGTGATCCGA GCTCAGGCAC CCTTGGAAAG 
GAGTGTGGGA GTGACTTTGC CTACTTCTAC TTTGTCTCCT TCATTTTCCT CTGCTCATTT 
CTGATGTTGA ACCTGTTTGT GGCCGTCATT ATGGACAACT TTGAATATCT GACACGCGAC 
TCCTCCATCC TGGGCCCTCA CCACCTGGAT GAGTTTATTC GAGTGTGGGC GGAGTATGAC 
CCAGCCGCAT GTGGCCGTAT CAAGTACCTC GATATGTATG AGATGCTGCT CCACATGTCC 
CCACCTTTAG GTTTGGGAAA GAAATGTCCT CCACGAATCG CCTACAAGCG TCTGGTGAAA 
ATGAACATGC CCATTGCGGA TGACAACAGT GTACACTTTA CCTCAACGCT GATGGCTCTG 
ATCCGTACTG CCCTGGAGAT CAAACTGGCT TCAGGCATGG TGGCTCAGCG TCTGTCAGAT 
GCTGAACTGA AGAAGGAGCT CTCTACAGTT TGGCCAAACC TGTCTCAGAA GACCATGGAC 
CTGCTGGTCA CTCCACATAA ACCTAATGAG CTCACAGTGG GGAAGGTGTA TGCAGCTCTT 
ATGATCTTTG ACTATTACAA GCAGAACAGA GCAAAAAGAC TTCAGATGCA GCAACAGCAG 
CTGAAAGAAC AGGCAGGACC TGGATCTCAG AATAAAGTCA GCGCTCTCTT AGAGCCCATT 
CTCCCACTAA CCCACATGCA AGATCAGCCA GTGAACGGTG TCGATTCAGC TTCAGAGTGT 
CAAGCTCAGA CCAGACCCAG CTCCACCACA CTCAACAATG GACGCACAAC TCTTGACAAC 
ACAATAAAAG GGTCTTCATC CTGGGCCTCA GAGAAATCCA AGGAAGTCCA AAAGTCTAAA 
CGGAGACCAA TGTCTAGAGG TCAATCTGAG GATGCCTCAA ATGCAAACAC TGCCCAGGAG 
TCAGTAGAAA TGAGAAAGAT GGAGAACAGT GCCAACACAG TGACCTCTGG TGGCCTTGAG 
GGCCAAGGAC GTGCAGCGTC CATGCCAAAA CTCAATGCTG AGATGCAGAG GAGTCATTCC 
CGTCAATCTC CTGGAACATT ACTGGAACCC ATTCCAGATT CCCCCATGAG GCGCTCTGCA 
TCCACTTTTG CACCACAGCG CCCTCACGAG GTGAATCTGA ATGACTACAT TCTGGAGAAA 
CCTGTTCAGG AACGCCATCA CCACCATCAC CATCATCACC GATGCCATCA TCGCCGGGAC 
AGAGACAAGA AACAGAGATC TCTGGACAGG TCGCCTACTG GCCATCACAA AACTACAGGC 
ACAGCCACTG ACTCTGTAGA GGAGAGGGCA CATGACCGGG GTCGTTCCCA TGAGAGGAAG 
CACCATTCAT CTTCAGGAGA GAAGCAGCGG TATTACTCGT GTGATCGCTA TGCCAGTCGG 
GAACACTGTT CCAGCAAATC TGCCGTGGCA AGCTGTGCCA CCTCACCCAG CGAGACCCAG 
GAGGCCAATC TTAACAAGCA GGGCAGTGGG TGGGCTAAGG GGAGCCCGGT GTTGTTGACT 
TCAGGGGCGA GCACTCCAAG CCGCGGGCGC AGACAGCTGC CCCAGACCCC CCTCACTCCT 
CGTCCCTCCG TGGCCTACAG GACTGCCAAC TCTTCCCCTG TGCAACTTCT GGCCACAACG 
ACCAGCTTGC TTCCCCCGAG TCCCGGCAGG CTGAGCCGTG GTCTGTCCGA GCACAACGCT 
CTTTTACTCA GCGGCTCTAT CTCCAGCTCG CCGGCCCCCG TCAGTCGCAT TAACTCAGAA 
CCCTTCTTGG GGCCCCCGGA CCACCCTGCT GGCTTGCAGC CCGAAGCCTT CGCTGTCTTC 
CAAGAGAATC CGAGCCCTCA GATGGGCCGT TCTCCTCAGA CTGTAGGGAT GCCCTTCGTG 
GCGATCCCCC CACCTCCCCC ACAGCAGTTT CGCAGGGTCC CCAACGGGTA CCACTTCTCC 
ACGGGCCCCA GCGCCAGCAG AGGACGGGCA CAGCAGTACT ACCCAGAGGC AGAGGTGGAG 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299770.1
CDS	636..7787
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPSYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301495.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301495.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGAGGAACC 
AAGGGCAAAG ACATCAGCAC TATCAAGTCA CTGAGGGTGT TGCGAGTCTT GCGACCTCTG 
AAGACCATCA AACGTCTACC AAAACTGAAG GCCGTGTTTG ATTGTGTGGT GAACTCCCTG 
AAGAATGTCC TGAATATCCT CATTGTCTAC ATTCTGTTCA TGTTCATTTT CGCTGTCATT 
GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT TACTGCACAG ACGAGTCCAA GGGCCTGGAG 
AAAGACTGCA GGGGGCTGTT TTTGGATTAT GATAAGGATG ATGTGACGGC ACAACCGAGG 
GAATGGAAGA AATACGAGTT TCACTATGAC AATGTGCTTT GGGCTTTTCT CACTCTCTTC 
ACTGTTTCTA CGGGAGAGGG CTGGCCTACG GTTTTGAAGC ACTCCGTGGA TGCCACTTTT 
GAGGATCAGG GTCCCAGTCC AGGCTACCGC ATTGAGATGT CTATTTTTTA CGTGGTCTAC 
TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC ATTTTTGTAG CCTTGATAAT CATCACATTC 
CAGGAACAGG GTGATAAGGT CTTGTCAGAG TGCAGTCTGG AGAAGAATGA GAGAGCCTGC 
ATCGACTTTG CTATCAATGC CAAACCTCTG ACACGCTACA TGCCGCAGAA CCAGCAGTCC 
TTTCAGTACC GCCTGTGGAA GTTTGTGGTC TCTCCGCCGT TTGAGTACTC CATCATGATC 
ATGATTGCTC TCAACACTGT GGTGCTCATG ATGAAGTTCC ATGGGGCGCC TGACTTTTAT 
GAGGCAATGC TTAAGTATCT CAACATTGTC TTCACTGTTC TCTTTTCACT GGAGTGTATT 
CTCAAGATTA TTGCTTTCGG CCCCTTGAAT TACCTGAAAG ATGCCTGGAA TGTGTTTGAC 
TTTGTGACTG TGCTGGGCAG CATCACTGAC ATTTTAGTCA CTGAGATTAA CACAACGGAG 
CGTCAGCTGA ACTTCAGTTT CCTGAGGCTC TTTAGAGCAG CCCGACTCAT CAAGCTCCTC 
CGGCAGGGAT ACACCATTCG CATCCTGCTC TGGACATTTG TCCAGTCTTT TAAGGCACTG 
CCTTATGTTT GCCTTCTAAT CGCCATGCTG TTTTTCATCT ACGCCATTAT TGGGATGCAG 
GTGTTTGGAA ACATTGATTT GAATGATGAC ACGGCAATCA ACCGTCACAA CAACTTCCGC 
ACTTTCCTCC AAGCTCTTAT GCTTTTGTTC AGGAGTGCCA CTGGCGAGGC TTGGCATGAT 
ATCATGCTGT CGTGTCTGAG CGAGCGCACC TGTGATCCGA GCTCAGGCAC CCTTGGAAAG 
GAGTGTGGGA GTGACTTTGC CTACTTCTAC TTTGTCTCCT TCATTTTCCT CTGCTCATTT 
CTGATGTTGA ACCTGTTTGT GGCCGTCATT ATGGACAACT TTGAATATCT GACACGCGAC 
TCCTCCATCC TGGGCCCTCA CCACCTGGAT GAGTTTATTC GAGTGTGGGC GGAGTATGAC 
CCAGCCGCAT GTGGCCGTAT CAAGTACCTC GATATGTATG AGATGCTGCT CCACATGTCC 
CCACCTTTAG GTTTGGGAAA GAAATGTCCT CCACGAATCG CCTACAAGCG TCTGGTGAAA 
ATGAACATGC CCATTGCGGA TGACAACAGT GTACACTTTA CCTCAACGCT GATGGCTCTG 
ATCCGTACTG CCCTGGAGAT CAAACTGGCT TCAGGCATGG TGGCTCAGCG TCTGTCAGAT 
GCTGAACTGA AGAAGGAGCT CTCTACAGTT TGGCCAAACC TGTCTCAGAA GACCATGGAC 
CTGCTGGTCA CTCCACATAA ACCTAATGAG CTCACAGTGG GGAAGGTGTA TGCAGCTCTT 
ATGATCTTTG ACTATTACAA GCAGAACAGA GCAAAAAGAC TTCAGATGCA GCAACAGCAG 
CTGAAAGAAC AGGCAGGACC TGGATCTCAG AATAAAGTCA GCGCTCTCTT AGAGCCCATT 
CTCCCACTAA CCCACATGCA AGATCAGCCA GTGAACGGTG TCGATTCAGC TTCAGAGTGT 
CAAGCTCAGA CCAGACCCAG CTCCACCACA CTCAACAATG GACGCACAAC TCTTGACAAC 
ACAATAAAAG GGTCTTCATC CTGGGCCTCA GAGAAATCCA AGGAAGTCCA AAAGTCTAAA 
CGGAGACCAA TGTCTAGAGG TCAATCTGAG GATGCCTCAA ATGCAAACAC TGCCCAGGAG 
TCAGTAGAAA TGAGAAAGAT GGAGAACAGT GCCAACACAG TGACCTCTGG TGGCCTTGAG 
GGCCAAGGAC GTGCAGCGTC CATGCCAAAA CTCAATGCTG AGATGCAGAG GAGTCATTCC 
CGTCAATCTC CTGGAACATT ACTGGAACCC ATTCCAGATT CCCCCATGAG GCGCTCTGCA 
TCCACTTTTG CACCACAGCG CCCTCACGAG GTGAATCTGA ATGACTACAT TCTGGAGAAA 
CCTGTTCAGG AACGCCATCA CCACCATCAC CATCATCACC GATGCCATCA TCGCCGGGAC 
AGAGACAAGA AACAGAGATC TCTGGACAGG TCGCCTACTG GCCATCACAA AACTACAGGC 
ACAGCCACTG ACTCTGTAGA GGAGAGGGCA CATGACCGGG GTCGTTCCCA TGAGAGGAAG 
CACCATTCAT CTTCAGGAGA GAAGCAGCGG TATTACTCGT GTGATCGCTA TGCCAGTCGG 
GAACACTGTT CCAGCAAATC TGCCGTGGCA AGCTGTGCCA CCTCACCCAG CGAGACCCAG 
GAGGCCAATC TTAACAAGCA GGGCAGTGGG TGGGCTAAGG GGAGCCCGGT GTTGTTGACT 
TCAGGGGCGA GCACTCCAAG CCGCGGGCGC AGACAGCTGC CCCAGACCCC CCTCACTCCT 
CGTCCCTCCG TGGCCTACAG GACTGCCAAC TCTTCCCCTG TGCAACTTCT GGCCACAACG 
ACCAGCTTGC TTCCCCCGAG TCCCGGCAGG CTGAGCCGTG GTCTGTCCGA GCACAACGCT 
CTTTTACTCA GCGGCTCTAT CTCCAGCTCG CCGGCCCCCG TCAGTCGCAT TAACTCAGAA 
CCCTTCTTGG GGCCCCCGGA CCACCCTGCT GGCTTGCAGC CCGAAGCCTT CGCTGTCTTC 
CAAGAGAATC CGAGCCCTCA GATGGGCCGT TCTCCTCAGA CTGTAGGGAT GCCCTTCGTG 
GCGATCCCCC CACCTCCCCC ACAGCAGTTT CGCAGGGTCC CCAACGGGTA CCACTTCTCC 
ACGGGCCCCA GCGCCAGCAG AGGACGGGCA CAGCAGTACT ACCCAGAGGC AGAGGTGGAG 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301496.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299771.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA.

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CloneID	ODa42260
Clone ID Related Accession (Same CDS sequence)	XM_009301496.2
Accession Version	XM_009301496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CAGGAACCAA GGGCAAAGAC 
ATCAGCACTA TCAAGTCACT GAGGGTGTTG CGAGTCTTGC GACCTCTGAA GACCATCAAA 
CGTCTACCAA AACTGAAGGC CGTGTTTGAT TGTGTGGTGA ACTCCCTGAA GAATGTCCTG 
AATATCCTCA TTGTCTACAT TCTGTTCATG TTCATTTTCG CTGTCATTGC TGTGCAGCTC 
TTCAAGGGCA AGTTTTTTTA CTGCACAGAC GAGTCCAAGG GCCTGGAGAA AGACTGCAGG 
GGGCTGTTTT TGGATTATGA TAAGGATGAT GTGACGGCAC AACCGAGGGA ATGGAAGAAA 
TACGAGTTTC ACTATGACAA TGTGCTTTGG GCTTTTCTCA CTCTCTTCAC TGTTTCTACG 
GGAGAGGGCT GGCCTACGGT TTTGAAGCAC TCCGTGGATG CCACTTTTGA GGATCAGGGT 
CCCAGTCCAG GCTACCGCAT TGAGATGTCT ATTTTTTACG TGGTCTACTT TGTGGTTTTC 
CCTTTCTTCT TCGTCAACAT TTTTGTAGCC TTGATAATCA TCACATTCCA GGAACAGGGT 
GATAAGGTCT TGTCAGAGTG CAGTCTGGAG AAGAATGAGA GAGCCTGCAT CGACTTTGCT 
ATCAATGCCA AACCTCTGAC ACGCTACATG CCGCAGAACC AGCAGTCCTT TCAGTACCGC 
CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT GAGTACTCCA TCATGATCAT GATTGCTCTC 
AACACTGTGG TGCTCATGAT GAAGTTCCAT GGGGCGCCTG ACTTTTATGA GGCAATGCTT 
AAGTATCTCA ACATTGTCTT CACTGTTCTC TTTTCACTGG AGTGTATTCT CAAGATTATT 
GCTTTCGGCC CCTTGAATTA CCTGAAAGAT GCCTGGAATG TGTTTGACTT TGTGACTGTG 
CTGGGCAGCA TCACTGACAT TTTAGTCACT GAGATTAACA CAACGGAGCG TCAGCTGAAC 
TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC CGACTCATCA AGCTCCTCCG GCAGGGATAC 
ACCATTCGCA TCCTGCTCTG GACATTTGTC CAGTCTTTTA AGGCACTGCC TTATGTTTGC 
CTTCTAATCG CCATGCTGTT TTTCATCTAC GCCATTATTG GGATGCAGGT GTTTGGAAAC 
ATTGATTTGA ATGATGACAC GGCAATCAAC CGTCACAACA ACTTCCGCAC TTTCCTCCAA 
GCTCTTATGC TTTTGTTCAG GAGTGCCACT GGCGAGGCTT GGCATGATAT CATGCTGTCG 
TGTCTGAGCG AGCGCACCTG TGATCCGAGC TCAGGCACCC TTGGAAAGGA GTGTGGGAGT 
GACTTTGCCT ACTTCTACTT TGTCTCCTTC ATTTTCCTCT GCTCATTTCT GATGTTGAAC 
CTGTTTGTGG CCGTCATTAT GGACAACTTT GAATATCTGA CACGCGACTC CTCCATCCTG 
GGCCCTCACC ACCTGGATGA GTTTATTCGA GTGTGGGCGG AGTATGACCC AGCCGCATGT 
GGCCGTATCA AGTACCTCGA TATGTATGAG ATGCTGCTCC ACATGTCCCC ACCTTTAGGT 
TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGTC TGGTGAAAAT GAACATGCCC 
ATTGCGGATG ACAACAGTGT ACACTTTACC TCAACGCTGA TGGCTCTGAT CCGTACTGCC 
CTGGAGATCA AACTGGCTTC AGGCATGGTG GCTCAGCGTC TGTCAGATGC TGAACTGAAG 
AAGGAGCTCT CTACAGTTTG GCCAAACCTG TCTCAGAAGA CCATGGACCT GCTGGTCACT 
CCACATAAAC CTAATGAGCT CACAGTGGGG AAGGTGTATG CAGCTCTTAT GATCTTTGAC 
TATTACAAGC AGAACAGAGC AAAAAGACTT CAGATGCAGC AACAGCAGCT GAAAGAACAG 
GCAGGACCTG GATCTCAGAA TAAAGTCAGC GCTCTCTTAG AGCCCATTCT CCCACTAACC 
CACATGCAAG ATCAGCCAGT GAACGGTGTC GATTCAGCTT CAGAGTGTCA AGCTCAGACC 
AGACCCAGCT CCACCACACT CAACAATGGA CGCACAACTC TTGACAACAC AATAAAAGGG 
TCTTCATCCT GGGCCTCAGA GAAATCCAAG GAAGTCCAAA AGTCTAAACG GAGACCAATG 
TCTAGAGGTC AATCTGAGGA TGCCTCAAAT GCAAACACTG CCCAGGAGTC AGTAGAAATG 
AGAAAGATGG AGAACAGTGC CAACACAGTG ACCTCTGGTG GCCTTGAGGG CCAAGGACGT 
GCAGCGTCCA TGCCAAAACT CAATGCTGAG ATGCAGAGGA GTCATTCCCG TCAATCTCCT 
GGAACATTAC TGGAACCCAT TCCAGATTCC CCCATGAGGC GCTCTGCATC CACTTTTGCA 
CCACAGCGCC CTCACGAGGT GAATCTGAAT GACTACATTC TGGAGAAACC TGTTCAGGAA 
CGCCATCACC ACCATCACCA TCATCACCGA TGCCATCATC GCCGGGACAG AGACAAGAAA 
CAGAGATCTC TGGACAGGTC GCCTACTGGC CATCACAAAA CTACAGGCAC AGCCACTGAC 
TCTGTAGAGG AGAGGGCACA TGACCGGGGT CGTTCCCATG AGAGGAAGCA CCATTCATCT 
TCAGGAGAGA AGCAGCGGTA TTACTCGTGT GATCGCTATG CCAGTCGGGA ACACTGTTCC 
AGCAAATCTG CCGTGGCAAG CTGTGCCACC TCACCCAGCG AGACCCAGGA GGCCAATCTT 
AACAAGCAGG GCAGTGGGTG GGCTAAGGGG AGCCCGGTGT TGTTGACTTC AGGGGCGAGC 
ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC CAGACCCCCC TCACTCCTCG TCCCTCCGTG 
GCCTACAGGA CTGCCAACTC TTCCCCTGTG CAACTTCTGG CCACAACGAC CAGCTTGCTT 
CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT CTGTCCGAGC ACAACGCTCT TTTACTCAGC 
GGCTCTATCT CCAGCTCGCC GGCCCCCGTC AGTCGCATTA ACTCAGAACC CTTCTTGGGG 
CCCCCGGACC ACCCTGCTGG CTTGCAGCCC GAAGCCTTCG CTGTCTTCCA AGAGAATCCG 
AGCCCTCAGA TGGGCCGTTC TCCTCAGACT GTAGGGATGC CCTTCGTGGC GATCCCCCCA 
CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC AACGGGTACC ACTTCTCCAC GGGCCCCAGC 
GCCAGCAGAG GACGGGCACA GCAGTACTAC CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299771.1
CDS	636..7775
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPSYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301496.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301496.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CAGGAACCAA GGGCAAAGAC 
ATCAGCACTA TCAAGTCACT GAGGGTGTTG CGAGTCTTGC GACCTCTGAA GACCATCAAA 
CGTCTACCAA AACTGAAGGC CGTGTTTGAT TGTGTGGTGA ACTCCCTGAA GAATGTCCTG 
AATATCCTCA TTGTCTACAT TCTGTTCATG TTCATTTTCG CTGTCATTGC TGTGCAGCTC 
TTCAAGGGCA AGTTTTTTTA CTGCACAGAC GAGTCCAAGG GCCTGGAGAA AGACTGCAGG 
GGGCTGTTTT TGGATTATGA TAAGGATGAT GTGACGGCAC AACCGAGGGA ATGGAAGAAA 
TACGAGTTTC ACTATGACAA TGTGCTTTGG GCTTTTCTCA CTCTCTTCAC TGTTTCTACG 
GGAGAGGGCT GGCCTACGGT TTTGAAGCAC TCCGTGGATG CCACTTTTGA GGATCAGGGT 
CCCAGTCCAG GCTACCGCAT TGAGATGTCT ATTTTTTACG TGGTCTACTT TGTGGTTTTC 
CCTTTCTTCT TCGTCAACAT TTTTGTAGCC TTGATAATCA TCACATTCCA GGAACAGGGT 
GATAAGGTCT TGTCAGAGTG CAGTCTGGAG AAGAATGAGA GAGCCTGCAT CGACTTTGCT 
ATCAATGCCA AACCTCTGAC ACGCTACATG CCGCAGAACC AGCAGTCCTT TCAGTACCGC 
CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT GAGTACTCCA TCATGATCAT GATTGCTCTC 
AACACTGTGG TGCTCATGAT GAAGTTCCAT GGGGCGCCTG ACTTTTATGA GGCAATGCTT 
AAGTATCTCA ACATTGTCTT CACTGTTCTC TTTTCACTGG AGTGTATTCT CAAGATTATT 
GCTTTCGGCC CCTTGAATTA CCTGAAAGAT GCCTGGAATG TGTTTGACTT TGTGACTGTG 
CTGGGCAGCA TCACTGACAT TTTAGTCACT GAGATTAACA CAACGGAGCG TCAGCTGAAC 
TTCAGTTTCC TGAGGCTCTT TAGAGCAGCC CGACTCATCA AGCTCCTCCG GCAGGGATAC 
ACCATTCGCA TCCTGCTCTG GACATTTGTC CAGTCTTTTA AGGCACTGCC TTATGTTTGC 
CTTCTAATCG CCATGCTGTT TTTCATCTAC GCCATTATTG GGATGCAGGT GTTTGGAAAC 
ATTGATTTGA ATGATGACAC GGCAATCAAC CGTCACAACA ACTTCCGCAC TTTCCTCCAA 
GCTCTTATGC TTTTGTTCAG GAGTGCCACT GGCGAGGCTT GGCATGATAT CATGCTGTCG 
TGTCTGAGCG AGCGCACCTG TGATCCGAGC TCAGGCACCC TTGGAAAGGA GTGTGGGAGT 
GACTTTGCCT ACTTCTACTT TGTCTCCTTC ATTTTCCTCT GCTCATTTCT GATGTTGAAC 
CTGTTTGTGG CCGTCATTAT GGACAACTTT GAATATCTGA CACGCGACTC CTCCATCCTG 
GGCCCTCACC ACCTGGATGA GTTTATTCGA GTGTGGGCGG AGTATGACCC AGCCGCATGT 
GGCCGTATCA AGTACCTCGA TATGTATGAG ATGCTGCTCC ACATGTCCCC ACCTTTAGGT 
TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGTC TGGTGAAAAT GAACATGCCC 
ATTGCGGATG ACAACAGTGT ACACTTTACC TCAACGCTGA TGGCTCTGAT CCGTACTGCC 
CTGGAGATCA AACTGGCTTC AGGCATGGTG GCTCAGCGTC TGTCAGATGC TGAACTGAAG 
AAGGAGCTCT CTACAGTTTG GCCAAACCTG TCTCAGAAGA CCATGGACCT GCTGGTCACT 
CCACATAAAC CTAATGAGCT CACAGTGGGG AAGGTGTATG CAGCTCTTAT GATCTTTGAC 
TATTACAAGC AGAACAGAGC AAAAAGACTT CAGATGCAGC AACAGCAGCT GAAAGAACAG 
GCAGGACCTG GATCTCAGAA TAAAGTCAGC GCTCTCTTAG AGCCCATTCT CCCACTAACC 
CACATGCAAG ATCAGCCAGT GAACGGTGTC GATTCAGCTT CAGAGTGTCA AGCTCAGACC 
AGACCCAGCT CCACCACACT CAACAATGGA CGCACAACTC TTGACAACAC AATAAAAGGG 
TCTTCATCCT GGGCCTCAGA GAAATCCAAG GAAGTCCAAA AGTCTAAACG GAGACCAATG 
TCTAGAGGTC AATCTGAGGA TGCCTCAAAT GCAAACACTG CCCAGGAGTC AGTAGAAATG 
AGAAAGATGG AGAACAGTGC CAACACAGTG ACCTCTGGTG GCCTTGAGGG CCAAGGACGT 
GCAGCGTCCA TGCCAAAACT CAATGCTGAG ATGCAGAGGA GTCATTCCCG TCAATCTCCT 
GGAACATTAC TGGAACCCAT TCCAGATTCC CCCATGAGGC GCTCTGCATC CACTTTTGCA 
CCACAGCGCC CTCACGAGGT GAATCTGAAT GACTACATTC TGGAGAAACC TGTTCAGGAA 
CGCCATCACC ACCATCACCA TCATCACCGA TGCCATCATC GCCGGGACAG AGACAAGAAA 
CAGAGATCTC TGGACAGGTC GCCTACTGGC CATCACAAAA CTACAGGCAC AGCCACTGAC 
TCTGTAGAGG AGAGGGCACA TGACCGGGGT CGTTCCCATG AGAGGAAGCA CCATTCATCT 
TCAGGAGAGA AGCAGCGGTA TTACTCGTGT GATCGCTATG CCAGTCGGGA ACACTGTTCC 
AGCAAATCTG CCGTGGCAAG CTGTGCCACC TCACCCAGCG AGACCCAGGA GGCCAATCTT 
AACAAGCAGG GCAGTGGGTG GGCTAAGGGG AGCCCGGTGT TGTTGACTTC AGGGGCGAGC 
ACTCCAAGCC GCGGGCGCAG ACAGCTGCCC CAGACCCCCC TCACTCCTCG TCCCTCCGTG 
GCCTACAGGA CTGCCAACTC TTCCCCTGTG CAACTTCTGG CCACAACGAC CAGCTTGCTT 
CCCCCGAGTC CCGGCAGGCT GAGCCGTGGT CTGTCCGAGC ACAACGCTCT TTTACTCAGC 
GGCTCTATCT CCAGCTCGCC GGCCCCCGTC AGTCGCATTA ACTCAGAACC CTTCTTGGGG 
CCCCCGGACC ACCCTGCTGG CTTGCAGCCC GAAGCCTTCG CTGTCTTCCA AGAGAATCCG 
AGCCCTCAGA TGGGCCGTTC TCCTCAGACT GTAGGGATGC CCTTCGTGGC GATCCCCCCA 
CCTCCCCCAC AGCAGTTTCG CAGGGTCCCC AACGGGTACC ACTTCTCCAC GGGCCCCAGC 
GCCAGCAGAG GACGGGCACA GCAGTACTAC CCAGAGGCAG AGGTGGAGGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301497.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299772.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA.

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CloneID	ODa42261
Clone ID Related Accession (Same CDS sequence)	XM_009301497.2 , XM_009301497.3
Accession Version	XM_009301497.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC 
AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC 
ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT 
CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT 
GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT 
CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT 
CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC 
TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG 
TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC 
CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC 
CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG 
GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT 
GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG 
GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG 
GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA 
CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT 
TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA 
GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC 
ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA 
CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT 
TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT 
AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA 
AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA 
GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA 
ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA 
CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC 
CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG 
AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT 
GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA 
GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC 
AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC 
AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT 
CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC 
TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC 
CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC 
TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC 
CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC 
CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT 
CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC 
AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299772.1
CDS	636..7772
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301497.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301497.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC 
AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC 
ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT 
CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT 
GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT 
CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT 
CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC 
TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG 
TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC 
CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC 
CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG 
GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT 
GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG 
GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG 
GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA 
CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT 
TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA 
GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC 
ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA 
CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT 
TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT 
AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA 
AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA 
GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA 
ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA 
CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC 
CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG 
AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT 
GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA 
GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC 
AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC 
AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT 
CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC 
TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC 
CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC 
TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC 
CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC 
CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT 
CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC 
AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301497.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299772.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA.

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CloneID	ODa42261
Clone ID Related Accession (Same CDS sequence)	XM_009301497.2 , XM_009301497.3
Accession Version	XM_009301497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC 
AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC 
ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT 
CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT 
GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT 
CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT 
CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC 
TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG 
TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC 
CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC 
CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG 
GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT 
GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG 
GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG 
GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA 
CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT 
TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA 
GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC 
ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA 
CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT 
TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT 
AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA 
AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA 
GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA 
ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA 
CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC 
CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG 
AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT 
GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA 
GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC 
AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC 
AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT 
CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC 
TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC 
CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC 
TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC 
CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC 
CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT 
CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC 
AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299772.1
CDS	637..7773
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301497.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301497.3

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCGAGCT TCATGGGATC ACATCAAAGC GTAAACAGAG GAACCAAGGG CAAAGACATC 
AGCACTATCA AGTCACTGAG GGTGTTGCGA GTCTTGCGAC CTCTGAAGAC CATCAAACGT 
CTACCAAAAC TGAAGGCCGT GTTTGATTGT GTGGTGAACT CCCTGAAGAA TGTCCTGAAT 
ATCCTCATTG TCTACATTCT GTTCATGTTC ATTTTCGCTG TCATTGCTGT GCAGCTCTTC 
AAGGGCAAGT TTTTTTACTG CACAGACGAG TCCAAGGGCC TGGAGAAAGA CTGCAGGGGG 
CTGTTTTTGG ATTATGATAA GGATGATGTG ACGGCACAAC CGAGGGAATG GAAGAAATAC 
GAGTTTCACT ATGACAATGT GCTTTGGGCT TTTCTCACTC TCTTCACTGT TTCTACGGGA 
GAGGGCTGGC CTACGGTTTT GAAGCACTCC GTGGATGCCA CTTTTGAGGA TCAGGGTCCC 
AGTCCAGGCT ACCGCATTGA GATGTCTATT TTTTACGTGG TCTACTTTGT GGTTTTCCCT 
TTCTTCTTCG TCAACATTTT TGTAGCCTTG ATAATCATCA CATTCCAGGA ACAGGGTGAT 
AAGGTCTTGT CAGAGTGCAG TCTGGAGAAG AATGAGAGAG CCTGCATCGA CTTTGCTATC 
AATGCCAAAC CTCTGACACG CTACATGCCG CAGAACCAGC AGTCCTTTCA GTACCGCCTG 
TGGAAGTTTG TGGTCTCTCC GCCGTTTGAG TACTCCATCA TGATCATGAT TGCTCTCAAC 
ACTGTGGTGC TCATGATGAA GTTCCATGGG GCGCCTGACT TTTATGAGGC AATGCTTAAG 
TATCTCAACA TTGTCTTCAC TGTTCTCTTT TCACTGGAGT GTATTCTCAA GATTATTGCT 
TTCGGCCCCT TGAATTACCT GAAAGATGCC TGGAATGTGT TTGACTTTGT GACTGTGCTG 
GGCAGCATCA CTGACATTTT AGTCACTGAG ATTAACACAA CGGAGCGTCA GCTGAACTTC 
AGTTTCCTGA GGCTCTTTAG AGCAGCCCGA CTCATCAAGC TCCTCCGGCA GGGATACACC 
ATTCGCATCC TGCTCTGGAC ATTTGTCCAG TCTTTTAAGG CACTGCCTTA TGTTTGCCTT 
CTAATCGCCA TGCTGTTTTT CATCTACGCC ATTATTGGGA TGCAGGTGTT TGGAAACATT 
GATTTGAATG ATGACACGGC AATCAACCGT CACAACAACT TCCGCACTTT CCTCCAAGCT 
CTTATGCTTT TGTTCAGGAG TGCCACTGGC GAGGCTTGGC ATGATATCAT GCTGTCGTGT 
CTGAGCGAGC GCACCTGTGA TCCGAGCTCA GGCACCCTTG GAAAGGAGTG TGGGAGTGAC 
TTTGCCTACT TCTACTTTGT CTCCTTCATT TTCCTCTGCT CATTTCTGAT GTTGAACCTG 
TTTGTGGCCG TCATTATGGA CAACTTTGAA TATCTGACAC GCGACTCCTC CATCCTGGGC 
CCTCACCACC TGGATGAGTT TATTCGAGTG TGGGCGGAGT ATGACCCAGC CGCATGTGGC 
CGTATCAAGT ACCTCGATAT GTATGAGATG CTGCTCCACA TGTCCCCACC TTTAGGTTTG 
GGAAAGAAAT GTCCTCCACG AATCGCCTAC AAGCGTCTGG TGAAAATGAA CATGCCCATT 
GCGGATGACA ACAGTGTACA CTTTACCTCA ACGCTGATGG CTCTGATCCG TACTGCCCTG 
GAGATCAAAC TGGCTTCAGG CATGGTGGCT CAGCGTCTGT CAGATGCTGA ACTGAAGAAG 
GAGCTCTCTA CAGTTTGGCC AAACCTGTCT CAGAAGACCA TGGACCTGCT GGTCACTCCA 
CATAAACCTA ATGAGCTCAC AGTGGGGAAG GTGTATGCAG CTCTTATGAT CTTTGACTAT 
TACAAGCAGA ACAGAGCAAA AAGACTTCAG ATGCAGCAAC AGCAGCTGAA AGAACAGGCA 
GGACCTGGAT CTCAGAATAA AGTCAGCGCT CTCTTAGAGC CCATTCTCCC ACTAACCCAC 
ATGCAAGATC AGCCAGTGAA CGGTGTCGAT TCAGCTTCAG AGTGTCAAGC TCAGACCAGA 
CCCAGCTCCA CCACACTCAA CAATGGACGC ACAACTCTTG ACAACACAAT AAAAGGGTCT 
TCATCCTGGG CCTCAGAGAA ATCCAAGGAA GTCCAAAAGT CTAAACGGAG ACCAATGTCT 
AGAGGTCAAT CTGAGGATGC CTCAAATGCA AACACTGCCC AGGAGTCAGT AGAAATGAGA 
AAGATGGAGA ACAGTGCCAA CACAGTGACC TCTGGTGGCC TTGAGGGCCA AGGACGTGCA 
GCGTCCATGC CAAAACTCAA TGCTGAGATG CAGAGGAGTC ATTCCCGTCA ATCTCCTGGA 
ACATTACTGG AACCCATTCC AGATTCCCCC ATGAGGCGCT CTGCATCCAC TTTTGCACCA 
CAGCGCCCTC ACGAGGTGAA TCTGAATGAC TACATTCTGG AGAAACCTGT TCAGGAACGC 
CATCACCACC ATCACCATCA TCACCGATGC CATCATCGCC GGGACAGAGA CAAGAAACAG 
AGATCTCTGG ACAGGTCGCC TACTGGCCAT CACAAAACTA CAGGCACAGC CACTGACTCT 
GTAGAGGAGA GGGCACATGA CCGGGGTCGT TCCCATGAGA GGAAGCACCA TTCATCTTCA 
GGAGAGAAGC AGCGGTATTA CTCGTGTGAT CGCTATGCCA GTCGGGAACA CTGTTCCAGC 
AAATCTGCCG TGGCAAGCTG TGCCACCTCA CCCAGCGAGA CCCAGGAGGC CAATCTTAAC 
AAGCAGGGCA GTGGGTGGGC TAAGGGGAGC CCGGTGTTGT TGACTTCAGG GGCGAGCACT 
CCAAGCCGCG GGCGCAGACA GCTGCCCCAG ACCCCCCTCA CTCCTCGTCC CTCCGTGGCC 
TACAGGACTG CCAACTCTTC CCCTGTGCAA CTTCTGGCCA CAACGACCAG CTTGCTTCCC 
CCGAGTCCCG GCAGGCTGAG CCGTGGTCTG TCCGAGCACA ACGCTCTTTT ACTCAGCGGC 
TCTATCTCCA GCTCGCCGGC CCCCGTCAGT CGCATTAACT CAGAACCCTT CTTGGGGCCC 
CCGGACCACC CTGCTGGCTT GCAGCCCGAA GCCTTCGCTG TCTTCCAAGA GAATCCGAGC 
CCTCAGATGG GCCGTTCTCC TCAGACTGTA GGGATGCCCT TCGTGGCGAT CCCCCCACCT 
CCCCCACAGC AGTTTCGCAG GGTCCCCAAC GGGTACCACT TCTCCACGGG CCCCAGCGCC 
AGCAGAGGAC GGGCACAGCA GTACTACCCA GAGGCAGAGG TGGAGGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301498.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299773.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA.

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CloneID	ODa42262
Clone ID Related Accession (Same CDS sequence)	XM_009301498.2
Accession Version	XM_009301498.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CAGGAACCAA GGGCAAAGAC 
ATCAGCACTA TCAAGTCACT GAGGGTGTTG CGAGTCTTGC GACCTCTGAA GACCATCAAA 
CGTCTACCAA AACTGAAGGC CGTGTTTGAT TGTGTGGTGA ACTCCCTGAA GAATGTCCTG 
AATATCCTCA TTGTCTACAT TCTGTTCATG TTCATTTTCG CTGTCATTGC TGTGCAGCTC 
TTCAAGGGCA AGTTTTTTTA CTGCACAGAC GAGTCCAAGG GCCTGGAGAA AGACTGCAGG 
GGGCTGTTTT TGGATTATGA TAAGGATGAT GTGACGGCAC AACCGAGGGA ATGGAAGAAA 
TACGAGTTTC ACTATGACAA TGTGCTTTGG GCTTTTCTCA CTCTCTTCAC TGTTTCTACG 
GGAGAGGGCT GGCCTACGGT TTTGAAGCAC TCCGTGGATG CCACTTTTGA GGATCAGGGT 
CCCAGTCCAG GCTACCGCAT TGAGATGTCT ATTTTTTACG TGGTCTACTT TGTGGTTTTC 
CCTTTCTTCT TCGTCAACAT TTTTGTAGCC TTGATAATCA TCACATTCCA GGAACAGGGT 
GATAAGGTCT TGTCAGAGTG CAGTCTGGAG AAGAATGAGA GAGCCTGCAT CGACTTTGCT 
ATCAATGCCA AACCTCTGAC ACGCTACATG CCGCAGAACC AGCAGTCCTT TCAGTACCGC 
CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT GAGTACTCCA TCATGATCAT GATTGCTCTC 
AACACTGTGG TGCTCATGAT GAAGTTCCAT GGGGCGCCTG ACTTTTATGA GGCAATGCTT 
AAGTATCTCA ACATTGTCTT CACTGTTCTC TTTTCACTGG AGTGTATTCT CAAGATTATT 
GCTTTCGGCC CCTTGAATTA CCTGAAAGAT GCCTGGAATG TGTTTGACTT TGTGACTGTG 
CTGGGCAGCA TCACTGACAT TTTAGTCACT GAGATTAACG AGCGTCAGCT GAACTTCAGT 
TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT 
CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA 
ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT 
TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT 
ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG 
AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT 
GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT 
GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT 
CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT 
ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA 
AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG 
GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG 
ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG 
CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT 
AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC 
AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA 
CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG 
CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC 
AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA 
TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA 
GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG 
ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG 
TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA 
TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG 
CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT 
CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA 
TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA 
GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA 
GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA 
TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG 
CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA 
AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC 
AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG 
AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT 
ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG 
GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT 
CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC 
CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC 
AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299773.1
CDS	637..7770
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPSYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301498.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301498.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CAGGAACCAA GGGCAAAGAC 
ATCAGCACTA TCAAGTCACT GAGGGTGTTG CGAGTCTTGC GACCTCTGAA GACCATCAAA 
CGTCTACCAA AACTGAAGGC CGTGTTTGAT TGTGTGGTGA ACTCCCTGAA GAATGTCCTG 
AATATCCTCA TTGTCTACAT TCTGTTCATG TTCATTTTCG CTGTCATTGC TGTGCAGCTC 
TTCAAGGGCA AGTTTTTTTA CTGCACAGAC GAGTCCAAGG GCCTGGAGAA AGACTGCAGG 
GGGCTGTTTT TGGATTATGA TAAGGATGAT GTGACGGCAC AACCGAGGGA ATGGAAGAAA 
TACGAGTTTC ACTATGACAA TGTGCTTTGG GCTTTTCTCA CTCTCTTCAC TGTTTCTACG 
GGAGAGGGCT GGCCTACGGT TTTGAAGCAC TCCGTGGATG CCACTTTTGA GGATCAGGGT 
CCCAGTCCAG GCTACCGCAT TGAGATGTCT ATTTTTTACG TGGTCTACTT TGTGGTTTTC 
CCTTTCTTCT TCGTCAACAT TTTTGTAGCC TTGATAATCA TCACATTCCA GGAACAGGGT 
GATAAGGTCT TGTCAGAGTG CAGTCTGGAG AAGAATGAGA GAGCCTGCAT CGACTTTGCT 
ATCAATGCCA AACCTCTGAC ACGCTACATG CCGCAGAACC AGCAGTCCTT TCAGTACCGC 
CTGTGGAAGT TTGTGGTCTC TCCGCCGTTT GAGTACTCCA TCATGATCAT GATTGCTCTC 
AACACTGTGG TGCTCATGAT GAAGTTCCAT GGGGCGCCTG ACTTTTATGA GGCAATGCTT 
AAGTATCTCA ACATTGTCTT CACTGTTCTC TTTTCACTGG AGTGTATTCT CAAGATTATT 
GCTTTCGGCC CCTTGAATTA CCTGAAAGAT GCCTGGAATG TGTTTGACTT TGTGACTGTG 
CTGGGCAGCA TCACTGACAT TTTAGTCACT GAGATTAACG AGCGTCAGCT GAACTTCAGT 
TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT 
CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA 
ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT 
TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT 
ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG 
AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT 
GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT 
GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT 
CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT 
ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA 
AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG 
GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG 
ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG 
CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT 
AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC 
AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA 
CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG 
CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC 
AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA 
TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA 
GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG 
ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG 
TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA 
TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG 
CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT 
CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA 
TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA 
GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA 
GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA 
TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG 
CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA 
AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC 
AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG 
AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT 
ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG 
GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT 
CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC 
CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC 
AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301499.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299774.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA.

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CloneID	ODa42263
Clone ID Related Accession (Same CDS sequence)	XM_009301499.2
Accession Version	XM_009301499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCGAGTTATG TCTGCTCCCC TGCACATCAC TACTTGAGTA AAGAACAGCA GCGTTCTCTA 
AAAATGATGT CCGTGTGGGA GCAGCGCACC ACTCAGATCC GAAAGCACAT GCTAGCCAGC 
AGTGAGGCGT TGTACCAAGA AGAGCTCAAT CCACGGCTCA TGTCTAACCT TCCTCTACGC 
ACCGACATAA AAACCCACCT GGACCGGCCT TTGGTTGTGG AGCCTCGCTG TGATGGCCCC 
ATCAGCCCAC ACAGTGGCTG GGACAAACCC CAGGATTTGC AAGGCGAGGG CAGCACACCA 
GAAGAACGAG TCCCACCTGT ATTCGAGCCT CCACAGTCAC ATCCACCACG CAAGCACCAC 
CGTCACAGAG AGCGTCTTTC TAATGAGACC AATGAAAATG GAGACACGGG TCATGCCAAA 
GAAGGCCGGC ATCACGTTCA TCACAGCCGA AGTAAAGAGC ATGATGGGAC ACGTTGTAAG 
GAGGGGAAGG GTGACAGGAG CCGAAGCAGA GAAGGGGGAC GTAGACATCA CCATCAGAGC 
TCGGTGGATG AGGGCGTAGG AGGTGGAGAG AGGGAACATC GGCACCATCA CTCCCATAGG 
CATAACAGAG AGGGCAATGG TGTGGTCAGT GCTGGGGGGA AGAGTGAACG GCGGTCCAGG 
CAGAAAGAGG GCTCACGATC AGGGACAAGA GAGGGAGAGA GATGCTCAAG GGGGGAGAAT 
GGAGGAAATG GAGGGAGGAG GCGACACAGG CCACGAACCA GTAAAGCTCA GTCTACGCTG 
GAAGGAGAGG AGCAACATGT GAATGGAGAG GAGGAGGGCA AGTCGCAAAG CCATAGGCAC 
ATGGATATGG GAGGAGATTC AATGGCTGTA AACCCCCATC AACCTTCAGT TGGAAGCCGG 
TGGTGTCTTG AGAAACCAGA AGATTCGGAC AACAAACGAA ATATTGGTCA GACTGGAGGA 
ATGAGCACCC ATATACCCGT GACTGTCACT TCTCCCCCGG GAGAAACAAC CCTTATTCCC 
ATGAACAGTA TAGACAGCGA GACAGTGCCC ATGACTGAGA AGAATCTAGA AGATCTAAAT 
CAAAGCAGCA TTCGTCCCAT CCTCCCCTTC AGCTCCATGT TCATTTTCAA CCCAACTAAT 
CCGGTGAGAC GTTTATGCCA CTACATTGTG AGTCTGCGCT ATTTTGAGAT GTGTATCCTG 
GTGGTCATTG CCATGAGCAG TATAGCCCTG GCTGCAGAAG ACCCGGTCCA AGCCAATGCA 
CCTCGGAACA ATGTCCTGAA GTATTTGGAC TATGTCTTCA CTGGTGTTTT CACATTCGAG 
ATGGTCATTA AGATGGTGGA TTTAGGTTTA ATTCTGCATC CTGGTTCCTA CTTTCGAGAT 
CTATGGAATA TTTTGGACTT CATTGTGGTC AGTGGTGCAC TGGTGGCATT CGCATTCTCG 
AGCTTCATGG GATCACATCA AAGCGTAAAC AGAGGAACCA AGGGCAAAGA CATCAGCACT 
ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG CGACCTCTGA AGACCATCAA ACGTCTACCA 
AAACTGAAGG CCGTGTTTGA TTGTGTGGTG AACTCCCTGA AGAATGTCCT GAATATCCTC 
ATTGTCTACA TTCTGTTCAT GTTCATTTTC GCTGTCATTG CTGTGCAGCT CTTCAAGGGC 
AAGTTTTTTT ACTGCACAGA CGAGTCCAAG GGCCTGGAGA AAGACTGCAG GGGGCTGTTT 
TTGGATTATG ATAAGGATGA TGTGACGGCA CAACCGAGGG AATGGAAGAA ATACGAGTTT 
CACTATGACA ATGTGCTTTG GGCTTTTCTC ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC 
TGGCCTACGG TTTTGAAGCA CTCCGTGGAT GCCACTTTTG AGGATCAGGG TCCCAGTCCA 
GGCTACCGCA TTGAGATGTC TATTTTTTAC GTGGTCTACT TTGTGGTTTT CCCTTTCTTC 
TTCGTCAACA TTTTTGTAGC CTTGATAATC ATCACATTCC AGGAACAGGG TGATAAGGTC 
TTGTCAGAGT GCAGTCTGGA GAAGAATGAG AGAGCCTGCA TCGACTTTGC TATCAATGCC 
AAACCTCTGA CACGCTACAT GCCGCAGAAC CAGCAGTCCT TTCAGTACCG CCTGTGGAAG 
TTTGTGGTCT CTCCGCCGTT TGAGTACTCC ATCATGATCA TGATTGCTCT CAACACTGTG 
GTGCTCATGA TGAAGTTCCA TGGGGCGCCT GACTTTTATG AGGCAATGCT TAAGTATCTC 
AACATTGTCT TCACTGTTCT CTTTTCACTG GAGTGTATTC TCAAGATTAT TGCTTTCGGC 
CCCTTGAATT ACCTGAAAGA TGCCTGGAAT GTGTTTGACT TTGTGACTGT GCTGGGCAGC 
ATCACTGACA TTTTAGTCAC TGAGATTAAC ACAACGGAGC GTCAGCTGAA CTTCAGTTTC 
CTGAGGCTCT TTAGAGCAGC CCGACTCATC AAGCTCCTCC GGCAGGGATA CACCATTCGC 
ATCCTGCTCT GGACATTTGT CCAGTCTTTT AAGGCACTGC CTTATGTTTG CCTTCTAATC 
GCCATGCTGT TTTTCATCTA CGCCATTATT GGGATGCAGG TGTTTGGAAA CATTGATTTG 
AATGATGACA CGGCAATCAA CCGTCACAAC AACTTCCGCA CTTTCCTCCA AGCTCTTATG 
CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT TGGCATGATA TCATGCTGTC GTGTCTGAGC 
GAGCGCACCT GTGATCCGAG CTCAGGCACC CTTGGAAAGG AGTGTGGGAG TGACTTTGCC 
TACTTCTACT TTGTCTCCTT CATTTTCCTC TGCTCATTTC TGATGTTGAA CCTGTTTGTG 
GCCGTCATTA TGGACAACTT TGAATATCTG ACACGCGACT CCTCCATCCT GGGCCCTCAC 
CACCTGGATG AGTTTATTCG AGTGTGGGCG GAGTATGACC CAGCCGCATG TGGCCGTATC 
AAGTACCTCG ATATGTATGA GATGCTGCTC CACATGTCCC CACCTTTAGG TTTGGGAAAG 
AAATGTCCTC CACGAATCGC CTACAAGCGT CTGGTGAAAA TGAACATGCC CATTGCGGAT 
GACAACAGTG TACACTTTAC CTCAACGCTG ATGGCTCTGA TCCGTACTGC CCTGGAGATC 
AAACTGGCTT CAGGCATGGT GGCTCAGCGT CTGTCAGATG CTGAACTGAA GAAGGAGCTC 
TCTACAGTTT GGCCAAACCT GTCTCAGAAG ACCATGGACC TGCTGGTCAC TCCACATAAA 
CCTAATGAGC TCACAGTGGG GAAGGTGTAT GCAGCTCTTA TGATCTTTGA CTATTACAAG 
CAGAACAGAG CAAAAAGACT TCAGATGCAG CAACAGCAGC TGAAAGAACA GGCAGGACCT 
GGATCTCAGA ATAAAGTCAG CGCTCTCTTA GAGCCCATTC TCCCACTAAC CCACATGCAA 
GATCAGCCAG TGAACGGTGT CGATTCAGCT TCAGAGTGTC AAGCTCAGAC CAGACCCAGC 
TCCACCACAC TCAACAATGG ACGCACAACT CTTGACAACA CAATAAAAGG GTCTTCATCC 
TGGGCCTCAG AGAAATCCAA GGAAGTCCAA AAGTCTAAAC GGAGACCAAT GTCTAGAGGT 
CAATCTGAGG ATGCCTCAAA TGCAAACACT GCCCAGGAGT CAGTAGAAAT GAGAAAGATG 
GAGAACAGTG CCAACACAGT GACCTCTGGT GGCCTTGAGG GCCAAGGACG TGCAGCGTCC 
ATGCCAAAAC TCAATGCTGA GATGCAGAGG AGTCATTCCC GTCAATCTCC TGGAACATTA 
CTGGAACCCA TTCCAGATTC CCCCATGAGG CGCTCTGCAT CCACTTTTGC ACCACAGCGC 
CCTCACGAGG TGAATCTGAA TGACTACATT CTGGAGAAAC CTGTTCAGGA ACGCCATCAC 
CACCATCACC ATCATCACCG ATGCCATCAT CGCCGGGACA GAGACAAGAA ACAGAGATCT 
CTGGACAGGT CGCCTACTGG CCATCACAAA ACTACAGGCA CAGCCACTGA CTCTGTAGAG 
GAGAGGGCAC ATGACCGGGG TCGTTCCCAT GAGAGGAAGC ACCATTCATC TTCAGGAGAG 
AAGCAGCGGT ATTACTCGTG TGATCGCTAT GCCAGTCGGG AACACTGTTC CAGCAAATCT 
GCCGTGGCAA GCTGTGCCAC CTCACCCAGC GAGACCCAGG AGGCCAATCT TAACAAGCAG 
GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG TTGTTGACTT CAGGGGCGAG CACTCCAAGC 
CGCGGGCGCA GACAGCTGCC CCAGACCCCC CTCACTCCTC GTCCCTCCGT GGCCTACAGG 
ACTGCCAACT CTTCCCCTGT GCAACTTCTG GCCACAACGA CCAGCTTGCT TCCCCCGAGT 
CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG CACAACGCTC TTTTACTCAG CGGCTCTATC 
TCCAGCTCGC CGGCCCCCGT CAGTCGCATT AACTCAGAAC CCTTCTTGGG GCCCCCGGAC 
CACCCTGCTG GCTTGCAGCC CGAAGCCTTC GCTGTCTTCC AAGAGAATCC GAGCCCTCAG 
ATGGGCCGTT CTCCTCAGAC TGTAGGGATG CCCTTCGTGG CGATCCCCCC ACCTCCCCCA 
CAGCAGTTTC GCAGGGTCCC CAACGGGTAC CACTTCTCCA CGGGCCCCAG CGCCAGCAGA 
GGACGGGCAC AGCAGTACTA CCCAGAGGCA GAGGTGGAGG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299774.1
CDS	636..7766
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPSYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301499.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301499.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCGAGTTATG TCTGCTCCCC TGCACATCAC TACTTGAGTA AAGAACAGCA GCGTTCTCTA 
AAAATGATGT CCGTGTGGGA GCAGCGCACC ACTCAGATCC GAAAGCACAT GCTAGCCAGC 
AGTGAGGCGT TGTACCAAGA AGAGCTCAAT CCACGGCTCA TGTCTAACCT TCCTCTACGC 
ACCGACATAA AAACCCACCT GGACCGGCCT TTGGTTGTGG AGCCTCGCTG TGATGGCCCC 
ATCAGCCCAC ACAGTGGCTG GGACAAACCC CAGGATTTGC AAGGCGAGGG CAGCACACCA 
GAAGAACGAG TCCCACCTGT ATTCGAGCCT CCACAGTCAC ATCCACCACG CAAGCACCAC 
CGTCACAGAG AGCGTCTTTC TAATGAGACC AATGAAAATG GAGACACGGG TCATGCCAAA 
GAAGGCCGGC ATCACGTTCA TCACAGCCGA AGTAAAGAGC ATGATGGGAC ACGTTGTAAG 
GAGGGGAAGG GTGACAGGAG CCGAAGCAGA GAAGGGGGAC GTAGACATCA CCATCAGAGC 
TCGGTGGATG AGGGCGTAGG AGGTGGAGAG AGGGAACATC GGCACCATCA CTCCCATAGG 
CATAACAGAG AGGGCAATGG TGTGGTCAGT GCTGGGGGGA AGAGTGAACG GCGGTCCAGG 
CAGAAAGAGG GCTCACGATC AGGGACAAGA GAGGGAGAGA GATGCTCAAG GGGGGAGAAT 
GGAGGAAATG GAGGGAGGAG GCGACACAGG CCACGAACCA GTAAAGCTCA GTCTACGCTG 
GAAGGAGAGG AGCAACATGT GAATGGAGAG GAGGAGGGCA AGTCGCAAAG CCATAGGCAC 
ATGGATATGG GAGGAGATTC AATGGCTGTA AACCCCCATC AACCTTCAGT TGGAAGCCGG 
TGGTGTCTTG AGAAACCAGA AGATTCGGAC AACAAACGAA ATATTGGTCA GACTGGAGGA 
ATGAGCACCC ATATACCCGT GACTGTCACT TCTCCCCCGG GAGAAACAAC CCTTATTCCC 
ATGAACAGTA TAGACAGCGA GACAGTGCCC ATGACTGAGA AGAATCTAGA AGATCTAAAT 
CAAAGCAGCA TTCGTCCCAT CCTCCCCTTC AGCTCCATGT TCATTTTCAA CCCAACTAAT 
CCGGTGAGAC GTTTATGCCA CTACATTGTG AGTCTGCGCT ATTTTGAGAT GTGTATCCTG 
GTGGTCATTG CCATGAGCAG TATAGCCCTG GCTGCAGAAG ACCCGGTCCA AGCCAATGCA 
CCTCGGAACA ATGTCCTGAA GTATTTGGAC TATGTCTTCA CTGGTGTTTT CACATTCGAG 
ATGGTCATTA AGATGGTGGA TTTAGGTTTA ATTCTGCATC CTGGTTCCTA CTTTCGAGAT 
CTATGGAATA TTTTGGACTT CATTGTGGTC AGTGGTGCAC TGGTGGCATT CGCATTCTCG 
AGCTTCATGG GATCACATCA AAGCGTAAAC AGAGGAACCA AGGGCAAAGA CATCAGCACT 
ATCAAGTCAC TGAGGGTGTT GCGAGTCTTG CGACCTCTGA AGACCATCAA ACGTCTACCA 
AAACTGAAGG CCGTGTTTGA TTGTGTGGTG AACTCCCTGA AGAATGTCCT GAATATCCTC 
ATTGTCTACA TTCTGTTCAT GTTCATTTTC GCTGTCATTG CTGTGCAGCT CTTCAAGGGC 
AAGTTTTTTT ACTGCACAGA CGAGTCCAAG GGCCTGGAGA AAGACTGCAG GGGGCTGTTT 
TTGGATTATG ATAAGGATGA TGTGACGGCA CAACCGAGGG AATGGAAGAA ATACGAGTTT 
CACTATGACA ATGTGCTTTG GGCTTTTCTC ACTCTCTTCA CTGTTTCTAC GGGAGAGGGC 
TGGCCTACGG TTTTGAAGCA CTCCGTGGAT GCCACTTTTG AGGATCAGGG TCCCAGTCCA 
GGCTACCGCA TTGAGATGTC TATTTTTTAC GTGGTCTACT TTGTGGTTTT CCCTTTCTTC 
TTCGTCAACA TTTTTGTAGC CTTGATAATC ATCACATTCC AGGAACAGGG TGATAAGGTC 
TTGTCAGAGT GCAGTCTGGA GAAGAATGAG AGAGCCTGCA TCGACTTTGC TATCAATGCC 
AAACCTCTGA CACGCTACAT GCCGCAGAAC CAGCAGTCCT TTCAGTACCG CCTGTGGAAG 
TTTGTGGTCT CTCCGCCGTT TGAGTACTCC ATCATGATCA TGATTGCTCT CAACACTGTG 
GTGCTCATGA TGAAGTTCCA TGGGGCGCCT GACTTTTATG AGGCAATGCT TAAGTATCTC 
AACATTGTCT TCACTGTTCT CTTTTCACTG GAGTGTATTC TCAAGATTAT TGCTTTCGGC 
CCCTTGAATT ACCTGAAAGA TGCCTGGAAT GTGTTTGACT TTGTGACTGT GCTGGGCAGC 
ATCACTGACA TTTTAGTCAC TGAGATTAAC ACAACGGAGC GTCAGCTGAA CTTCAGTTTC 
CTGAGGCTCT TTAGAGCAGC CCGACTCATC AAGCTCCTCC GGCAGGGATA CACCATTCGC 
ATCCTGCTCT GGACATTTGT CCAGTCTTTT AAGGCACTGC CTTATGTTTG CCTTCTAATC 
GCCATGCTGT TTTTCATCTA CGCCATTATT GGGATGCAGG TGTTTGGAAA CATTGATTTG 
AATGATGACA CGGCAATCAA CCGTCACAAC AACTTCCGCA CTTTCCTCCA AGCTCTTATG 
CTTTTGTTCA GGAGTGCCAC TGGCGAGGCT TGGCATGATA TCATGCTGTC GTGTCTGAGC 
GAGCGCACCT GTGATCCGAG CTCAGGCACC CTTGGAAAGG AGTGTGGGAG TGACTTTGCC 
TACTTCTACT TTGTCTCCTT CATTTTCCTC TGCTCATTTC TGATGTTGAA CCTGTTTGTG 
GCCGTCATTA TGGACAACTT TGAATATCTG ACACGCGACT CCTCCATCCT GGGCCCTCAC 
CACCTGGATG AGTTTATTCG AGTGTGGGCG GAGTATGACC CAGCCGCATG TGGCCGTATC 
AAGTACCTCG ATATGTATGA GATGCTGCTC CACATGTCCC CACCTTTAGG TTTGGGAAAG 
AAATGTCCTC CACGAATCGC CTACAAGCGT CTGGTGAAAA TGAACATGCC CATTGCGGAT 
GACAACAGTG TACACTTTAC CTCAACGCTG ATGGCTCTGA TCCGTACTGC CCTGGAGATC 
AAACTGGCTT CAGGCATGGT GGCTCAGCGT CTGTCAGATG CTGAACTGAA GAAGGAGCTC 
TCTACAGTTT GGCCAAACCT GTCTCAGAAG ACCATGGACC TGCTGGTCAC TCCACATAAA 
CCTAATGAGC TCACAGTGGG GAAGGTGTAT GCAGCTCTTA TGATCTTTGA CTATTACAAG 
CAGAACAGAG CAAAAAGACT TCAGATGCAG CAACAGCAGC TGAAAGAACA GGCAGGACCT 
GGATCTCAGA ATAAAGTCAG CGCTCTCTTA GAGCCCATTC TCCCACTAAC CCACATGCAA 
GATCAGCCAG TGAACGGTGT CGATTCAGCT TCAGAGTGTC AAGCTCAGAC CAGACCCAGC 
TCCACCACAC TCAACAATGG ACGCACAACT CTTGACAACA CAATAAAAGG GTCTTCATCC 
TGGGCCTCAG AGAAATCCAA GGAAGTCCAA AAGTCTAAAC GGAGACCAAT GTCTAGAGGT 
CAATCTGAGG ATGCCTCAAA TGCAAACACT GCCCAGGAGT CAGTAGAAAT GAGAAAGATG 
GAGAACAGTG CCAACACAGT GACCTCTGGT GGCCTTGAGG GCCAAGGACG TGCAGCGTCC 
ATGCCAAAAC TCAATGCTGA GATGCAGAGG AGTCATTCCC GTCAATCTCC TGGAACATTA 
CTGGAACCCA TTCCAGATTC CCCCATGAGG CGCTCTGCAT CCACTTTTGC ACCACAGCGC 
CCTCACGAGG TGAATCTGAA TGACTACATT CTGGAGAAAC CTGTTCAGGA ACGCCATCAC 
CACCATCACC ATCATCACCG ATGCCATCAT CGCCGGGACA GAGACAAGAA ACAGAGATCT 
CTGGACAGGT CGCCTACTGG CCATCACAAA ACTACAGGCA CAGCCACTGA CTCTGTAGAG 
GAGAGGGCAC ATGACCGGGG TCGTTCCCAT GAGAGGAAGC ACCATTCATC TTCAGGAGAG 
AAGCAGCGGT ATTACTCGTG TGATCGCTAT GCCAGTCGGG AACACTGTTC CAGCAAATCT 
GCCGTGGCAA GCTGTGCCAC CTCACCCAGC GAGACCCAGG AGGCCAATCT TAACAAGCAG 
GGCAGTGGGT GGGCTAAGGG GAGCCCGGTG TTGTTGACTT CAGGGGCGAG CACTCCAAGC 
CGCGGGCGCA GACAGCTGCC CCAGACCCCC CTCACTCCTC GTCCCTCCGT GGCCTACAGG 
ACTGCCAACT CTTCCCCTGT GCAACTTCTG GCCACAACGA CCAGCTTGCT TCCCCCGAGT 
CCCGGCAGGC TGAGCCGTGG TCTGTCCGAG CACAACGCTC TTTTACTCAG CGGCTCTATC 
TCCAGCTCGC CGGCCCCCGT CAGTCGCATT AACTCAGAAC CCTTCTTGGG GCCCCCGGAC 
CACCCTGCTG GCTTGCAGCC CGAAGCCTTC GCTGTCTTCC AAGAGAATCC GAGCCCTCAG 
ATGGGCCGTT CTCCTCAGAC TGTAGGGATG CCCTTCGTGG CGATCCCCCC ACCTCCCCCA 
CAGCAGTTTC GCAGGGTCCC CAACGGGTAC CACTTCTCCA CGGGCCCCAG CGCCAGCAGA 
GGACGGGCAC AGCAGTACTA CCCAGAGGCA GAGGTGGAGG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301500.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299775.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA.

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CloneID	ODa42264
Clone ID Related Accession (Same CDS sequence)	XM_009301500.2 , XM_009301500.3
Accession Version	XM_009301500.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC 
ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT 
TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT 
ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC 
AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG 
GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC 
ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC 
CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT 
CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG 
GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG 
CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG 
CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG 
CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG 
AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG 
TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG 
CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC 
TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA 
GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA 
ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC 
CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC 
ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT 
GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG 
AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG 
AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC 
ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT 
CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC 
AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC 
CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC 
TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC 
AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG 
GTGTTGTTGA CTTCAGGGGC GAGCACTCCA AGCCGCGGGC GCAGACAGCT GCCCCAGACC 
CCCCTCACTC CTCGTCCCTC CGTGGCCTAC AGGACTGCCA ACTCTTCCCC TGTGCAACTT 
CTGGCCACAA CGACCAGCTT GCTTCCCCCG AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC 
GAGCACAACG CTCTTTTACT CAGCGGCTCT ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC 
ATTAACTCAG AACCCTTCTT GGGGCCCCCG GACCACCCTG CTGGCTTGCA GCCCGAAGCC 
TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT CAGATGGGCC GTTCTCCTCA GACTGTAGGG 
ATGCCCTTCG TGGCGATCCC CCCACCTCCC CCACAGCAGT TTCGCAGGGT CCCCAACGGG 
TACCACTTCT CCACGGGCCC CAGCGCCAGC AGAGGACGGG CACAGCAGTA CTACCCAGAG 
GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299775.1
CDS	636..7739
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301500.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301500.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC 
ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT 
TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT 
ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC 
AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG 
GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC 
ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC 
CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT 
CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG 
GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG 
CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG 
CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG 
CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG 
AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG 
TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG 
CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC 
TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA 
GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA 
ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC 
CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC 
ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT 
GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG 
AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG 
AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC 
ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT 
CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC 
AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC 
CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC 
TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC 
AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG 
GTGTTGTTGA CTTCAGGGGC GAGCACTCCA AGCCGCGGGC GCAGACAGCT GCCCCAGACC 
CCCCTCACTC CTCGTCCCTC CGTGGCCTAC AGGACTGCCA ACTCTTCCCC TGTGCAACTT 
CTGGCCACAA CGACCAGCTT GCTTCCCCCG AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC 
GAGCACAACG CTCTTTTACT CAGCGGCTCT ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC 
ATTAACTCAG AACCCTTCTT GGGGCCCCCG GACCACCCTG CTGGCTTGCA GCCCGAAGCC 
TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT CAGATGGGCC GTTCTCCTCA GACTGTAGGG 
ATGCCCTTCG TGGCGATCCC CCCACCTCCC CCACAGCAGT TTCGCAGGGT CCCCAACGGG 
TACCACTTCT CCACGGGCCC CAGCGCCAGC AGAGGACGGG CACAGCAGTA CTACCCAGAG 
GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301500.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299775.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA.

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CloneID	ODa42264
Clone ID Related Accession (Same CDS sequence)	XM_009301500.2 , XM_009301500.3
Accession Version	XM_009301500.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC 
ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT 
TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT 
ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC 
AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG 
GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC 
ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC 
CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT 
CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG 
GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG 
CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG 
CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG 
CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG 
AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG 
TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG 
CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC 
TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA 
GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA 
ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC 
CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC 
ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT 
GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG 
AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG 
AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC 
ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT 
CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC 
AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC 
CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC 
TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC 
AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG 
GTGTTGTTGA CTTCAGGGGC GAGCACTCCA AGCCGCGGGC GCAGACAGCT GCCCCAGACC 
CCCCTCACTC CTCGTCCCTC CGTGGCCTAC AGGACTGCCA ACTCTTCCCC TGTGCAACTT 
CTGGCCACAA CGACCAGCTT GCTTCCCCCG AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC 
GAGCACAACG CTCTTTTACT CAGCGGCTCT ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC 
ATTAACTCAG AACCCTTCTT GGGGCCCCCG GACCACCCTG CTGGCTTGCA GCCCGAAGCC 
TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT CAGATGGGCC GTTCTCCTCA GACTGTAGGG 
ATGCCCTTCG TGGCGATCCC CCCACCTCCC CCACAGCAGT TTCGCAGGGT CCCCAACGGG 
TACCACTTCT CCACGGGCCC CAGCGCCAGC AGAGGACGGG CACAGCAGTA CTACCCAGAG 
GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299775.1
CDS	1..7104
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301500.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301500.3

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC 
ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT 
TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT 
ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC 
AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG 
GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC 
ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC 
CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT 
CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG 
GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG 
CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG 
CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG 
CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG 
AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG 
TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG 
CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC 
TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA 
GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA 
ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC 
CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC 
ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT 
GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG 
AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG 
AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC 
ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT 
CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC 
AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC 
CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC 
TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC 
AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG 
GTGTTGTTGA CTTCAGGGGC GAGCACTCCA AGCCGCGGGC GCAGACAGCT GCCCCAGACC 
CCCCTCACTC CTCGTCCCTC CGTGGCCTAC AGGACTGCCA ACTCTTCCCC TGTGCAACTT 
CTGGCCACAA CGACCAGCTT GCTTCCCCCG AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC 
GAGCACAACG CTCTTTTACT CAGCGGCTCT ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC 
ATTAACTCAG AACCCTTCTT GGGGCCCCCG GACCACCCTG CTGGCTTGCA GCCCGAAGCC 
TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT CAGATGGGCC GTTCTCCTCA GACTGTAGGG 
ATGCCCTTCG TGGCGATCCC CCCACCTCCC CCACAGCAGT TTCGCAGGGT CCCCAACGGG 
TACCACTTCT CCACGGGCCC CAGCGCCAGC AGAGGACGGG CACAGCAGTA CTACCCAGAG 
GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301501.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299776.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA.

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CloneID	ODa42265
Clone ID Related Accession (Same CDS sequence)	XM_009301501.3 , XM_009301501.2
Accession Version	XM_009301501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC 
ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA 
CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC 
CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG 
GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG 
TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG 
TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG 
GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT 
CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC 
TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG 
GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT 
GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG 
AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT 
GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT 
CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC 
GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC 
AGCATCACTG ACATTTTAGT CACTGAGATT AACACAACGG AGCGTCAGCT GAACTTCAGT 
TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT 
CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA 
ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT 
TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT 
ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG 
AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT 
GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT 
GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT 
CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT 
ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA 
AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG 
GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG 
ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG 
CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT 
AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC 
AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA 
CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG 
CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC 
AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA 
TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA 
GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG 
ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG 
TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA 
TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG 
CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT 
CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA 
TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA 
GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA 
GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA 
TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG 
CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA 
AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC 
AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG 
AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT 
ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG 
GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT 
CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC 
CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC 
AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299776.1
CDS	1..7074
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301501.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301501.3

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC 
ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA 
CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC 
CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG 
GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG 
TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG 
TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG 
GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT 
CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC 
TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG 
GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT 
GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG 
AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT 
GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT 
CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC 
GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC 
AGCATCACTG ACATTTTAGT CACTGAGATT AACACAACGG AGCGTCAGCT GAACTTCAGT 
TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT 
CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA 
ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT 
TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT 
ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG 
AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT 
GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT 
GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT 
CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT 
ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA 
AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG 
GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG 
ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG 
CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT 
AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC 
AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA 
CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG 
CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC 
AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA 
TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA 
GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG 
ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG 
TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA 
TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG 
CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT 
CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA 
TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA 
GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA 
GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA 
TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG 
CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA 
AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC 
AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG 
AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT 
ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG 
GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT 
CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC 
CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC 
AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301501.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299776.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA.

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CloneID	ODa42265
Clone ID Related Accession (Same CDS sequence)	XM_009301501.3 , XM_009301501.2
Accession Version	XM_009301501.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC 
ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA 
CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC 
CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG 
GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG 
TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG 
TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG 
GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT 
CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC 
TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG 
GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT 
GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG 
AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT 
GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT 
CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC 
GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC 
AGCATCACTG ACATTTTAGT CACTGAGATT AACACAACGG AGCGTCAGCT GAACTTCAGT 
TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT 
CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA 
ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT 
TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT 
ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG 
AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT 
GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT 
GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT 
CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT 
ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA 
AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG 
GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG 
ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG 
CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT 
AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC 
AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA 
CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG 
CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC 
AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA 
TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA 
GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG 
ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG 
TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA 
TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG 
CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT 
CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA 
TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA 
GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA 
GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA 
TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG 
CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA 
AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC 
AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG 
AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT 
ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG 
GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT 
CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC 
CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC 
AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299776.1
CDS	636..7709
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301501.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301501.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTAAAGAACA GCAGCGTTCT 
CTAAAAATGA TGTCCGTGTG GGAGCAGCGC ACCACTCAGA TCCGAAAGCA CATGCTAGCC 
AGCAGTGAGG CGTTGTACCA AGAAGAGCTC AATCCACGGC TCATGTCTAA CCTTCCTCTA 
CGCACCGACA TAAAAACCCA CCTGGACCGG CCTTTGGTTG TGGAGCCTCG CTGTGATGGC 
CCCATCAGCC CACACAGTGG CTGGGACAAA CCCCAGGATT TGCAAGGCGA GGGCAGCACA 
CCAGAAGAAC GAGTCCCACC TGTATTCGAG CCTCCACAGT CACATCCACC ACGCAAGCAC 
CACCGTCACA GAGAGCGTCT TTCTAATGAG ACCAATGAAA ATGGAGACAC GGGTCATGCC 
AAAGAAGGCC GGCATCACGT TCATCACAGC CGAAGTAAAG AGCATGATGG GACACGTTGT 
AAGGAGGGGA AGGGTGACAG GAGCCGAAGC AGAGAAGGGG GACGTAGACA TCACCATCAG 
AGCTCGGTGG ATGAGGGCGT AGGAGGTGGA GAGAGGGAAC ATCGGCACCA TCACTCCCAT 
AGGCATAACA GAGAGGGCAA TGGTGTGGTC AGTGCTGGGG GGAAGAGTGA ACGGCGGTCC 
AGGCAGAAAG AGGGCTCACG ATCAGGGACA AGAGAGGGAG AGAGATGCTC AAGGGGGGAG 
AATGGAGGAA ATGGAGGGAG GAGGCGACAC AGGCCACGAA CCAGTAAAGC TCAGTCTACG 
CTGGAAGGAG AGGAGCAACA TGTGAATGGA GAGGAGGAGG GCAAGTCGCA AAGCCATAGG 
CACATGGATA TGGGAGGAGA TTCAATGGCT GTAAACCCCC ATCAACCTTC AGTTGGAAGC 
CGGTGGTGTC TTGAGAAACC AGAAGATTCG GACAACAAAC GAAATATTGG TCAGACTGGA 
GGAATGAGCA CCCATATACC CGTGACTGTC ACTTCTCCCC CGGGAGAAAC AACCCTTATT 
CCCATGAACA GTATAGACAG CGAGACAGTG CCCATGACTG AGAAGAATCT AGAAGATCTA 
AATCAAAGCA GCATTCGTCC CATCCTCCCC TTCAGCTCCA TGTTCATTTT CAACCCAACT 
AATCCGGTGA GACGTTTATG CCACTACATT GTGAGTCTGC GCTATTTTGA GATGTGTATC 
CTGGTGGTCA TTGCCATGAG CAGTATAGCC CTGGCTGCAG AAGACCCGGT CCAAGCCAAT 
GCACCTCGGA ACAATGTCCT GAAGTATTTG GACTATGTCT TCACTGGTGT TTTCACATTC 
GAGATGGTCA TTAAGATGGT GGATTTAGGT TTAATTCTGC ATCCTGGTTC CTACTTTCGA 
GATCTATGGA ATATTTTGGA CTTCATTGTG GTCAGTGGTG CACTGGTGGC ATTCGCATTC 
TCGAGCTTCA TGGGATCACA TCAAAGCGTA AACAGAGGAA CCAAGGGCAA AGACATCAGC 
ACTATCAAGT CACTGAGGGT GTTGCGAGTC TTGCGACCTC TGAAGACCAT CAAACGTCTA 
CCAAAACTGA AGGCCGTGTT TGATTGTGTG GTGAACTCCC TGAAGAATGT CCTGAATATC 
CTCATTGTCT ACATTCTGTT CATGTTCATT TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG 
GGCAAGTTTT TTTACTGCAC AGACGAGTCC AAGGGCCTGG AGAAAGACTG CAGGGGGCTG 
TTTTTGGATT ATGATAAGGA TGATGTGACG GCACAACCGA GGGAATGGAA GAAATACGAG 
TTTCACTATG ACAATGTGCT TTGGGCTTTT CTCACTCTCT TCACTGTTTC TACGGGAGAG 
GGCTGGCCTA CGGTTTTGAA GCACTCCGTG GATGCCACTT TTGAGGATCA GGGTCCCAGT 
CCAGGCTACC GCATTGAGAT GTCTATTTTT TACGTGGTCT ACTTTGTGGT TTTCCCTTTC 
TTCTTCGTCA ACATTTTTGT AGCCTTGATA ATCATCACAT TCCAGGAACA GGGTGATAAG 
GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT GAGAGAGCCT GCATCGACTT TGCTATCAAT 
GCCAAACCTC TGACACGCTA CATGCCGCAG AACCAGCAGT CCTTTCAGTA CCGCCTGTGG 
AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC TCCATCATGA TCATGATTGC TCTCAACACT 
GTGGTGCTCA TGATGAAGTT CCATGGGGCG CCTGACTTTT ATGAGGCAAT GCTTAAGTAT 
CTCAACATTG TCTTCACTGT TCTCTTTTCA CTGGAGTGTA TTCTCAAGAT TATTGCTTTC 
GGCCCCTTGA ATTACCTGAA AGATGCCTGG AATGTGTTTG ACTTTGTGAC TGTGCTGGGC 
AGCATCACTG ACATTTTAGT CACTGAGATT AACACAACGG AGCGTCAGCT GAACTTCAGT 
TTCCTGAGGC TCTTTAGAGC AGCCCGACTC ATCAAGCTCC TCCGGCAGGG ATACACCATT 
CGCATCCTGC TCTGGACATT TGTCCAGTCT TTTAAGGCAC TGCCTTATGT TTGCCTTCTA 
ATCGCCATGC TGTTTTTCAT CTACGCCATT ATTGGGATGC AGGTGTTTGG AAACATTGAT 
TTGAATGATG ACACGGCAAT CAACCGTCAC AACAACTTCC GCACTTTCCT CCAAGCTCTT 
ATGCTTTTGT TCAGGAGTGC CACTGGCGAG GCTTGGCATG ATATCATGCT GTCGTGTCTG 
AGCGAGCGCA CCTGTGATCC GAGCTCAGGC ACCCTTGGAA AGGAGTGTGG GAGTGACTTT 
GCCTACTTCT ACTTTGTCTC CTTCATTTTC CTCTGCTCAT TTCTGATGTT GAACCTGTTT 
GTGGCCGTCA TTATGGACAA CTTTGAATAT CTGACACGCG ACTCCTCCAT CCTGGGCCCT 
CACCACCTGG ATGAGTTTAT TCGAGTGTGG GCGGAGTATG ACCCAGCCGC ATGTGGCCGT 
ATCAAGTACC TCGATATGTA TGAGATGCTG CTCCACATGT CCCCACCTTT AGGTTTGGGA 
AAGAAATGTC CTCCACGAAT CGCCTACAAG CGTCTGGTGA AAATGAACAT GCCCATTGCG 
GATGACAACA GTGTACACTT TACCTCAACG CTGATGGCTC TGATCCGTAC TGCCCTGGAG 
ATCAAACTGG CTTCAGGCAT GGTGGCTCAG CGTCTGTCAG ATGCTGAACT GAAGAAGGAG 
CTCTCTACAG TTTGGCCAAA CCTGTCTCAG AAGACCATGG ACCTGCTGGT CACTCCACAT 
AAACCTAATG AGCTCACAGT GGGGAAGGTG TATGCAGCTC TTATGATCTT TGACTATTAC 
AAGCAGAACA GAGCAAAAAG ACTTCAGATG CAGCAACAGC AGCTGAAAGA ACAGGCAGGA 
CCTGGATCTC AGAATAAAGT CAGCGCTCTC TTAGAGCCCA TTCTCCCACT AACCCACATG 
CAAGATCAGC CAGTGAACGG TGTCGATTCA GCTTCAGAGT GTCAAGCTCA GACCAGACCC 
AGCTCCACCA CACTCAACAA TGGACGCACA ACTCTTGACA ACACAATAAA AGGGTCTTCA 
TCCTGGGCCT CAGAGAAATC CAAGGAAGTC CAAAAGTCTA AACGGAGACC AATGTCTAGA 
GGTCAATCTG AGGATGCCTC AAATGCAAAC ACTGCCCAGG AGTCAGTAGA AATGAGAAAG 
ATGGAGAACA GTGCCAACAC AGTGACCTCT GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG 
TCCATGCCAA AACTCAATGC TGAGATGCAG AGGAGTCATT CCCGTCAATC TCCTGGAACA 
TTACTGGAAC CCATTCCAGA TTCCCCCATG AGGCGCTCTG CATCCACTTT TGCACCACAG 
CGCCCTCACG AGGTGAATCT GAATGACTAC ATTCTGGAGA AACCTGTTCA GGAACGCCAT 
CACCACCATC ACCATCATCA CCGATGCCAT CATCGCCGGG ACAGAGACAA GAAACAGAGA 
TCTCTGGACA GGTCGCCTAC TGGCCATCAC AAAACTACAG GCACAGCCAC TGACTCTGTA 
GAGGAGAGGG CACATGACCG GGGTCGTTCC CATGAGAGGA AGCACCATTC ATCTTCAGGA 
GAGAAGCAGC GGTATTACTC GTGTGATCGC TATGCCAGTC GGGAACACTG TTCCAGCAAA 
TCTGCCGTGG CAAGCTGTGC CACCTCACCC AGCGAGACCC AGGAGGCCAA TCTTAACAAG 
CAGGGCAGTG GGTGGGCTAA GGGGAGCCCG GTGTTGTTGA CTTCAGGGGC GAGCACTCCA 
AGCCGCGGGC GCAGACAGCT GCCCCAGACC CCCCTCACTC CTCGTCCCTC CGTGGCCTAC 
AGGACTGCCA ACTCTTCCCC TGTGCAACTT CTGGCCACAA CGACCAGCTT GCTTCCCCCG 
AGTCCCGGCA GGCTGAGCCG TGGTCTGTCC GAGCACAACG CTCTTTTACT CAGCGGCTCT 
ATCTCCAGCT CGCCGGCCCC CGTCAGTCGC ATTAACTCAG AACCCTTCTT GGGGCCCCCG 
GACCACCCTG CTGGCTTGCA GCCCGAAGCC TTCGCTGTCT TCCAAGAGAA TCCGAGCCCT 
CAGATGGGCC GTTCTCCTCA GACTGTAGGG ATGCCCTTCG TGGCGATCCC CCCACCTCCC 
CCACAGCAGT TTCGCAGGGT CCCCAACGGG TACCACTTCT CCACGGGCCC CAGCGCCAGC 
AGAGGACGGG CACAGCAGTA CTACCCAGAG GCAGAGGTGG AGGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301503.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299778.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA.

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CloneID	ODa42266
Clone ID Related Accession (Same CDS sequence)	XM_009301503.2
Accession Version	XM_009301503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT 
CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG 
TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT 
GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC 
ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA 
GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT 
GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT 
TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG 
CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG 
TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA 
GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG 
GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC 
ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG 
TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC 
CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT 
CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA 
GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC 
ACTGAGATTA ACACAACGGA GCGTCAGCTG AACTTCAGTT TCCTGAGGCT CTTTAGAGCA 
GCCCGACTCA TCAAGCTCCT CCGGCAGGGA TACACCATTC GCATCCTGCT CTGGACATTT 
GTCCAGTCTT TTAAGGCACT GCCTTATGTT TGCCTTCTAA TCGCCATGCT GTTTTTCATC 
TACGCCATTA TTGGGATGCA GGTGTTTGGA AACATTGATT TGAATGATGA CACGGCAATC 
AACCGTCACA ACAACTTCCG CACTTTCCTC CAAGCTCTTA TGCTTTTGTT CAGGAGTGCC 
ACTGGCGAGG CTTGGCATGA TATCATGCTG TCGTGTCTGA GCGAGCGCAC CTGTGATCCG 
AGCTCAGGCA CCCTTGGAAA GGAGTGTGGG AGTGACTTTG CCTACTTCTA CTTTGTCTCC 
TTCATTTTCC TCTGCTCATT TCTGATGTTG AACCTGTTTG TGGCCGTCAT TATGGACAAC 
TTTGAATATC TGACACGCGA CTCCTCCATC CTGGGCCCTC ACCACCTGGA TGAGTTTATT 
CGAGTGTGGG CGGAGTATGA CCCAGCCGCA TGTGGCCGTA TCAAGTACCT CGATATGTAT 
GAGATGCTGC TCCACATGTC CCCACCTTTA GGTTTGGGAA AGAAATGTCC TCCACGAATC 
GCCTACAAGC GTCTGGTGAA AATGAACATG CCCATTGCGG ATGACAACAG TGTACACTTT 
ACCTCAACGC TGATGGCTCT GATCCGTACT GCCCTGGAGA TCAAACTGGC TTCAGGCATG 
GTGGCTCAGC GTCTGTCAGA TGCTGAACTG AAGAAGGAGC TCTCTACAGT TTGGCCAAAC 
CTGTCTCAGA AGACCATGGA CCTGCTGGTC ACTCCACATA AACCTAATGA GCTCACAGTG 
GGGAAGGTGT ATGCAGCTCT TATGATCTTT GACTATTACA AGCAGAACAG AGCAAAAAGA 
CTTCAGATGC AGCAACAGCA GCTGAAAGAA CAGGCAGGAC CTGGATCTCA GAATAAAGTC 
AGCGCTCTCT TAGAGCCCAT TCTCCCACTA ACCCACATGC AAGATCAGCC AGTGAACGGT 
GTCGATTCAG CTTCAGAGTG TCAAGCTCAG ACCAGACCCA GCTCCACCAC ACTCAACAAT 
GGACGCACAA CTCTTGACAA CACAATAAAA GGGTCTTCAT CCTGGGCCTC AGAGAAATCC 
AAGGAAGTCC AAAAGTCTAA ACGGAGACCA ATGTCTAGAG GTCAATCTGA GGATGCCTCA 
AATGCAAACA CTGCCCAGGA GTCAGTAGAA ATGAGAAAGA TGGAGAACAG TGCCAACACA 
GTGACCTCTG GTGGCCTTGA GGGCCAAGGA CGTGCAGCGT CCATGCCAAA ACTCAATGCT 
GAGATGCAGA GGAGTCATTC CCGTCAATCT CCTGGAACAT TACTGGAACC CATTCCAGAT 
TCCCCCATGA GGCGCTCTGC ATCCACTTTT GCACCACAGC GCCCTCACGA GGTGAATCTG 
AATGACTACA TTCTGGAGAA ACCTGTTCAG GAACGCCATC ACCACCATCA CCATCATCAC 
CGATGCCATC ATCGCCGGGA CAGAGACAAG AAACAGAGAT CTCTGGACAG GTCGCCTACT 
GGCCATCACA AAACTACAGG CACAGCCACT GACTCTGTAG AGGAGAGGGC ACATGACCGG 
GGTCGTTCCC ATGAGAGGAA GCACCATTCA TCTTCAGGAG AGAAGCAGCG GTATTACTCG 
TGTGATCGCT ATGCCAGTCG GGAACACTGT TCCAGCAAAT CTGCCGTGGC AAGCTGTGCC 
ACCTCACCCA GCGAGACCCA GGAGGCCAAT CTTAACAAGC AGTGGGTGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299778.1
CDS	639..7292
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPSYVCSPAHHYLSKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGSHQSVNRGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQWVG

Target ORF information:

RefSeq Version	XM_009301503.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301503.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCGAGTTA TGTCTGCTCC 
CCTGCACATC ACTACTTGAG TAAAGAACAG CAGCGTTCTC TAAAAATGAT GTCCGTGTGG 
GAGCAGCGCA CCACTCAGAT CCGAAAGCAC ATGCTAGCCA GCAGTGAGGC GTTGTACCAA 
GAAGAGCTCA ATCCACGGCT CATGTCTAAC CTTCCTCTAC GCACCGACAT AAAAACCCAC 
CTGGACCGGC CTTTGGTTGT GGAGCCTCGC TGTGATGGCC CCATCAGCCC ACACAGTGGC 
TGGGACAAAC CCCAGGATTT GCAAGGCGAG GGCAGCACAC CAGAAGAACG AGTCCCACCT 
GTATTCGAGC CTCCACAGTC ACATCCACCA CGCAAGCACC ACCGTCACAG AGAGCGTCTT 
TCTAATGAGA CCAATGAAAA TGGAGACACG GGTCATGCCA AAGAAGGCCG GCATCACGTT 
CATCACAGCC GAAGTAAAGA GCATGATGGG ACACGTTGTA AGGAGGGGAA GGGTGACAGG 
AGCCGAAGCA GAGAAGGGGG ACGTAGACAT CACCATCAGA GCTCGGTGGA TGAGGGCGTA 
GGAGGTGGAG AGAGGGAACA TCGGCACCAT CACTCCCATA GGCATAACAG AGAGGGCAAT 
GGTGTGGTCA GTGCTGGGGG GAAGAGTGAA CGGCGGTCCA GGCAGAAAGA GGGCTCACGA 
TCAGGGACAA GAGAGGGAGA GAGATGCTCA AGGGGGGAGA ATGGAGGAAA TGGAGGGAGG 
AGGCGACACA GGCCACGAAC CAGTAAAGCT CAGTCTACGC TGGAAGGAGA GGAGCAACAT 
GTGAATGGAG AGGAGGAGGG CAAGTCGCAA AGCCATAGGC ACATGGATAT GGGAGGAGAT 
TCAATGGCTG TAAACCCCCA TCAACCTTCA GTTGGAAGCC GGTGGTGTCT TGAGAAACCA 
GAAGATTCGG ACAACAAACG AAATATTGGT CAGACTGGAG GAATGAGCAC CCATATACCC 
GTGACTGTCA CTTCTCCCCC GGGAGAAACA ACCCTTATTC CCATGAACAG TATAGACAGC 
GAGACAGTGC CCATGACTGA GAAGAATCTA GAAGATCTAA ATCAAAGCAG CATTCGTCCC 
ATCCTCCCCT TCAGCTCCAT GTTCATTTTC AACCCAACTA ATCCGGTGAG ACGTTTATGC 
CACTACATTG TGAGTCTGCG CTATTTTGAG ATGTGTATCC TGGTGGTCAT TGCCATGAGC 
AGTATAGCCC TGGCTGCAGA AGACCCGGTC CAAGCCAATG CACCTCGGAA CAATGTCCTG 
AAGTATTTGG ACTATGTCTT CACTGGTGTT TTCACATTCG AGATGGTCAT TAAGATGGTG 
GATTTAGGTT TAATTCTGCA TCCTGGTTCC TACTTTCGAG ATCTATGGAA TATTTTGGAC 
TTCATTGTGG TCAGTGGTGC ACTGGTGGCA TTCGCATTCT CGAGCTTCAT GGGATCACAT 
CAAAGCGTAA ACAGAGGAAC CAAGGGCAAA GACATCAGCA CTATCAAGTC ACTGAGGGTG 
TTGCGAGTCT TGCGACCTCT GAAGACCATC AAACGTCTAC CAAAACTGAA GGCCGTGTTT 
GATTGTGTGG TGAACTCCCT GAAGAATGTC CTGAATATCC TCATTGTCTA CATTCTGTTC 
ATGTTCATTT TCGCTGTCAT TGCTGTGCAG CTCTTCAAGG GCAAGTTTTT TTACTGCACA 
GACGAGTCCA AGGGCCTGGA GAAAGACTGC AGGGGGCTGT TTTTGGATTA TGATAAGGAT 
GATGTGACGG CACAACCGAG GGAATGGAAG AAATACGAGT TTCACTATGA CAATGTGCTT 
TGGGCTTTTC TCACTCTCTT CACTGTTTCT ACGGGAGAGG GCTGGCCTAC GGTTTTGAAG 
CACTCCGTGG ATGCCACTTT TGAGGATCAG GGTCCCAGTC CAGGCTACCG CATTGAGATG 
TCTATTTTTT ACGTGGTCTA CTTTGTGGTT TTCCCTTTCT TCTTCGTCAA CATTTTTGTA 
GCCTTGATAA TCATCACATT CCAGGAACAG GGTGATAAGG TCTTGTCAGA GTGCAGTCTG 
GAGAAGAATG AGAGAGCCTG CATCGACTTT GCTATCAATG CCAAACCTCT GACACGCTAC 
ATGCCGCAGA ACCAGCAGTC CTTTCAGTAC CGCCTGTGGA AGTTTGTGGT CTCTCCGCCG 
TTTGAGTACT CCATCATGAT CATGATTGCT CTCAACACTG TGGTGCTCAT GATGAAGTTC 
CATGGGGCGC CTGACTTTTA TGAGGCAATG CTTAAGTATC TCAACATTGT CTTCACTGTT 
CTCTTTTCAC TGGAGTGTAT TCTCAAGATT ATTGCTTTCG GCCCCTTGAA TTACCTGAAA 
GATGCCTGGA ATGTGTTTGA CTTTGTGACT GTGCTGGGCA GCATCACTGA CATTTTAGTC 
ACTGAGATTA ACACAACGGA GCGTCAGCTG AACTTCAGTT TCCTGAGGCT CTTTAGAGCA 
GCCCGACTCA TCAAGCTCCT CCGGCAGGGA TACACCATTC GCATCCTGCT CTGGACATTT 
GTCCAGTCTT TTAAGGCACT GCCTTATGTT TGCCTTCTAA TCGCCATGCT GTTTTTCATC 
TACGCCATTA TTGGGATGCA GGTGTTTGGA AACATTGATT TGAATGATGA CACGGCAATC 
AACCGTCACA ACAACTTCCG CACTTTCCTC CAAGCTCTTA TGCTTTTGTT CAGGAGTGCC 
ACTGGCGAGG CTTGGCATGA TATCATGCTG TCGTGTCTGA GCGAGCGCAC CTGTGATCCG 
AGCTCAGGCA CCCTTGGAAA GGAGTGTGGG AGTGACTTTG CCTACTTCTA CTTTGTCTCC 
TTCATTTTCC TCTGCTCATT TCTGATGTTG AACCTGTTTG TGGCCGTCAT TATGGACAAC 
TTTGAATATC TGACACGCGA CTCCTCCATC CTGGGCCCTC ACCACCTGGA TGAGTTTATT 
CGAGTGTGGG CGGAGTATGA CCCAGCCGCA TGTGGCCGTA TCAAGTACCT CGATATGTAT 
GAGATGCTGC TCCACATGTC CCCACCTTTA GGTTTGGGAA AGAAATGTCC TCCACGAATC 
GCCTACAAGC GTCTGGTGAA AATGAACATG CCCATTGCGG ATGACAACAG TGTACACTTT 
ACCTCAACGC TGATGGCTCT GATCCGTACT GCCCTGGAGA TCAAACTGGC TTCAGGCATG 
GTGGCTCAGC GTCTGTCAGA TGCTGAACTG AAGAAGGAGC TCTCTACAGT TTGGCCAAAC 
CTGTCTCAGA AGACCATGGA CCTGCTGGTC ACTCCACATA AACCTAATGA GCTCACAGTG 
GGGAAGGTGT ATGCAGCTCT TATGATCTTT GACTATTACA AGCAGAACAG AGCAAAAAGA 
CTTCAGATGC AGCAACAGCA GCTGAAAGAA CAGGCAGGAC CTGGATCTCA GAATAAAGTC 
AGCGCTCTCT TAGAGCCCAT TCTCCCACTA ACCCACATGC AAGATCAGCC AGTGAACGGT 
GTCGATTCAG CTTCAGAGTG TCAAGCTCAG ACCAGACCCA GCTCCACCAC ACTCAACAAT 
GGACGCACAA CTCTTGACAA CACAATAAAA GGGTCTTCAT CCTGGGCCTC AGAGAAATCC 
AAGGAAGTCC AAAAGTCTAA ACGGAGACCA ATGTCTAGAG GTCAATCTGA GGATGCCTCA 
AATGCAAACA CTGCCCAGGA GTCAGTAGAA ATGAGAAAGA TGGAGAACAG TGCCAACACA 
GTGACCTCTG GTGGCCTTGA GGGCCAAGGA CGTGCAGCGT CCATGCCAAA ACTCAATGCT 
GAGATGCAGA GGAGTCATTC CCGTCAATCT CCTGGAACAT TACTGGAACC CATTCCAGAT 
TCCCCCATGA GGCGCTCTGC ATCCACTTTT GCACCACAGC GCCCTCACGA GGTGAATCTG 
AATGACTACA TTCTGGAGAA ACCTGTTCAG GAACGCCATC ACCACCATCA CCATCATCAC 
CGATGCCATC ATCGCCGGGA CAGAGACAAG AAACAGAGAT CTCTGGACAG GTCGCCTACT 
GGCCATCACA AAACTACAGG CACAGCCACT GACTCTGTAG AGGAGAGGGC ACATGACCGG 
GGTCGTTCCC ATGAGAGGAA GCACCATTCA TCTTCAGGAG AGAAGCAGCG GTATTACTCG 
TGTGATCGCT ATGCCAGTCG GGAACACTGT TCCAGCAAAT CTGCCGTGGC AAGCTGTGCC 
ACCTCACCCA GCGAGACCCA GGAGGCCAAT CTTAACAAGC AGTGGGTGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301504.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299779.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA.

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CloneID	ODa42267
Clone ID Related Accession (Same CDS sequence)	XM_009301504.3 , XM_009301504.2
Accession Version	XM_009301504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC 
ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT 
TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT 
ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC 
AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG 
GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC 
ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC 
CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT 
CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG 
GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG 
CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG 
CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG 
CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG 
AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG 
TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG 
CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC 
TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA 
GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA 
ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC 
CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC 
ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT 
GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG 
AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG 
AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC 
ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT 
CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC 
AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC 
CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC 
TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC 
AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGTGGGTGG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299779.1
CDS	1..6585
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQWVG

Target ORF information:

RefSeq Version	XM_009301504.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301504.3

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC 
ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT 
TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT 
ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC 
AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG 
GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC 
ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC 
CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT 
CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG 
GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG 
CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG 
CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG 
CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG 
AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG 
TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG 
CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC 
TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA 
GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA 
ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC 
CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC 
ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT 
GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG 
AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG 
AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC 
ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT 
CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC 
AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC 
CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC 
TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC 
AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGTGGGTGG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301504.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299779.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X14, mRNA.

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CloneID	ODa42267
Clone ID Related Accession (Same CDS sequence)	XM_009301504.3 , XM_009301504.2
Accession Version	XM_009301504.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC 
ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT 
TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT 
ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC 
AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG 
GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC 
ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC 
CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT 
CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG 
GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG 
CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG 
CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG 
CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG 
AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG 
TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG 
CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC 
TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA 
GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA 
ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC 
CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC 
ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT 
GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG 
AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG 
AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC 
ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT 
CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC 
AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC 
CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC 
TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC 
AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGTGGGTGG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299779.1
CDS	639..7223
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPGGFTRSLGADFGLPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINTTERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQWVG

Target ORF information:

RefSeq Version	XM_009301504.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301504.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGGTGGTTTT 
ACTCGCTCAC TTGGAGCAGA CTTTGGGTTG CCAGTTTTGA AGCGGGCCAA GAAGAGCAAA 
AATGACCTCA TCAATGCAGA GGAAGGAGAA GATCACTTTA CTGACATCTC TTCAGTTGCT 
CCCCAAGGCT CTCCATTCAC CCGCACAAGT GTGAAGAGCA GCAAGATTGA GAGCTTGTCG 
TACTTTCGCA GAAAAGAAAA GAGGTTTCGC TTCTTCATCC GCCGCATGGT GAAGGCTCAG 
AGTTTCTACT GGACTGTTCT GTGTATAGTG GGACTGAACA CACTGTGTGT GGCCATAGTC 
CACTACGACC AGCCCGAATG GCTCACATAT GCACTGTATT TGGCGGAGTT TGTGTTCCTT 
GGTCTGTTCC TAATAGAGAT GAGTCTGAAG ATGTATGGCC TCGGGCCCAG GACTTACTTT 
CACTCTTCAT TTAACTGCTT TGACTTTGGG GTGATTGTGG GGAGCATTTT CGAGGTGATC 
TGGGCTGCAG TAAAGCCAGG AGCTTCCTTT GGCATTAGTG TGTTGCGAGC TCTTCGTTTG 
CTAAGAATAT TCAAGGTTAC CAAGTATTGG AACTCATTGA GGAATCTGGT AGTGTCTCTG 
CTCAACTCAA TGAAGTCCAT CATTAGTCTC CTGTTTCTGC TGTTCCTCTT CATCGTGGTG 
TTTGCTCTGT TGGGAATGCA GCTCTTTGGG GGACAGTTTA ATTTTGAAGA CGAGACCCCG 
ACAACCAACT TTGACACATT CCCAGCTGCT ATCTTGACAG TGTTTCAGAT TTTGACTGGA 
GAGGACTGGA ACGCTGTGAT GTATCATGGG ATTGAATCGC AGGGCGGAGT GCACCGGGGA 
ATGTTCTGCT CCGTGTACTT CATCGTGCTC ACGCTGTTTG GAAACTACAC TCTCCTCAAT 
GTCTTTTTGG CCATCGCTGT TGATAACCTT GCCAATGCAC AAGAACTCAC CAAGGATGAG 
GAGGAGCAAG AGGAGGCTAT CAGTAAGAAA CTGGCTCTAC AGAAAGCCAA GGAGGTAAAG 
GAGGTCAGCC CCATGTCGGC AGCTAACATT TCCATCACAG CTTTTATAAG GCCAAACCCT 
GGTGCTCTTT CATGCAGCTC CTCCAACTCC AGCTCTCATT CACCTAAAGA ACAGCAGCGT 
TCTCTAAAAA TGATGTCCGT GTGGGAGCAG CGCACCACTC AGATCCGAAA GCACATGCTA 
GCCAGCAGTG AGGCGTTGTA CCAAGAAGAG CTCAATCCAC GGCTCATGTC TAACCTTCCT 
CTACGCACCG ACATAAAAAC CCACCTGGAC CGGCCTTTGG TTGTGGAGCC TCGCTGTGAT 
GGCCCCATCA GCCCACACAG TGGCTGGGAC AAACCCCAGG ATTTGCAAGG CGAGGGCAGC 
ACACCAGAAG AACGAGTCCC ACCTGTATTC GAGCCTCCAC AGTCACATCC ACCACGCAAG 
CACCACCGTC ACAGAGAGCG TCTTTCTAAT GAGACCAATG AAAATGGAGA CACGGGTCAT 
GCCAAAGAAG GCCGGCATCA CGTTCATCAC AGCCGAAGTA AAGAGCATGA TGGGACACGT 
TGTAAGGAGG GGAAGGGTGA CAGGAGCCGA AGCAGAGAAG GGGGACGTAG ACATCACCAT 
CAGAGCTCGG TGGATGAGGG CGTAGGAGGT GGAGAGAGGG AACATCGGCA CCATCACTCC 
CATAGGCATA ACAGAGAGGG CAATGGTGTG GTCAGTGCTG GGGGGAAGAG TGAACGGCGG 
TCCAGGCAGA AAGAGGGCTC ACGATCAGGG ACAAGAGAGG GAGAGAGATG CTCAAGGGGG 
GAGAATGGAG GAAATGGAGG GAGGAGGCGA CACAGGCCAC GAACCAGTAA AGCTCAGTCT 
ACGCTGGAAG GAGAGGAGCA ACATGTGAAT GGAGAGGAGG AGGGCAAGTC GCAAAGCCAT 
AGGCACATGG ATATGGGAGG AGATTCAATG GCTGTAAACC CCCATCAACC TTCAGTTGGA 
AGCCGGTGGT GTCTTGAGAA ACCAGAAGAT TCGGACAACA AACGAAATAT TGGTCAGACT 
GGAGGAATGA GCACCCATAT ACCCGTGACT GTCACTTCTC CCCCGGGAGA AACAACCCTT 
ATTCCCATGA ACAGTATAGA CAGCGAGACA GTGCCCATGA CTGAGAAGAA TCTAGAAGAT 
CTAAATCAAA GCAGCATTCG TCCCATCCTC CCCTTCAGCT CCATGTTCAT TTTCAACCCA 
ACTAATCCGG TGAGACGTTT ATGCCACTAC ATTGTGAGTC TGCGCTATTT TGAGATGTGT 
ATCCTGGTGG TCATTGCCAT GAGCAGTATA GCCCTGGCTG CAGAAGACCC GGTCCAAGCC 
AATGCACCTC GGAACAATGT CCTGAAGTAT TTGGACTATG TCTTCACTGG TGTTTTCACA 
TTCGAGATGG TCATTAAGAT GGTGGATTTA GGTTTAATTC TGCATCCTGG TTCCTACTTT 
CGAGATCTAT GGAATATTTT GGACTTCATT GTGGTCAGTG GTGCACTGGT GGCATTCGCA 
TTCTCAGGAA CCAAGGGCAA AGACATCAGC ACTATCAAGT CACTGAGGGT GTTGCGAGTC 
TTGCGACCTC TGAAGACCAT CAAACGTCTA CCAAAACTGA AGGCCGTGTT TGATTGTGTG 
GTGAACTCCC TGAAGAATGT CCTGAATATC CTCATTGTCT ACATTCTGTT CATGTTCATT 
TTCGCTGTCA TTGCTGTGCA GCTCTTCAAG GGCAAGTTTT TTTACTGCAC AGACGAGTCC 
AAGGGCCTGG AGAAAGACTG CAGGGGGCTG TTTTTGGATT ATGATAAGGA TGATGTGACG 
GCACAACCGA GGGAATGGAA GAAATACGAG TTTCACTATG ACAATGTGCT TTGGGCTTTT 
CTCACTCTCT TCACTGTTTC TACGGGAGAG GGCTGGCCTA CGGTTTTGAA GCACTCCGTG 
GATGCCACTT TTGAGGATCA GGGTCCCAGT CCAGGCTACC GCATTGAGAT GTCTATTTTT 
TACGTGGTCT ACTTTGTGGT TTTCCCTTTC TTCTTCGTCA ACATTTTTGT AGCCTTGATA 
ATCATCACAT TCCAGGAACA GGGTGATAAG GTCTTGTCAG AGTGCAGTCT GGAGAAGAAT 
GAGAGAGCCT GCATCGACTT TGCTATCAAT GCCAAACCTC TGACACGCTA CATGCCGCAG 
AACCAGCAGT CCTTTCAGTA CCGCCTGTGG AAGTTTGTGG TCTCTCCGCC GTTTGAGTAC 
TCCATCATGA TCATGATTGC TCTCAACACT GTGGTGCTCA TGATGAAGTT CCATGGGGCG 
CCTGACTTTT ATGAGGCAAT GCTTAAGTAT CTCAACATTG TCTTCACTGT TCTCTTTTCA 
CTGGAGTGTA TTCTCAAGAT TATTGCTTTC GGCCCCTTGA ATTACCTGAA AGATGCCTGG 
AATGTGTTTG ACTTTGTGAC TGTGCTGGGC AGCATCACTG ACATTTTAGT CACTGAGATT 
AACACAACGG AGCGTCAGCT GAACTTCAGT TTCCTGAGGC TCTTTAGAGC AGCCCGACTC 
ATCAAGCTCC TCCGGCAGGG ATACACCATT CGCATCCTGC TCTGGACATT TGTCCAGTCT 
TTTAAGGCAC TGCCTTATGT TTGCCTTCTA ATCGCCATGC TGTTTTTCAT CTACGCCATT 
ATTGGGATGC AGGTGTTTGG AAACATTGAT TTGAATGATG ACACGGCAAT CAACCGTCAC 
AACAACTTCC GCACTTTCCT CCAAGCTCTT ATGCTTTTGT TCAGGAGTGC CACTGGCGAG 
GCTTGGCATG ATATCATGCT GTCGTGTCTG AGCGAGCGCA CCTGTGATCC GAGCTCAGGC 
ACCCTTGGAA AGGAGTGTGG GAGTGACTTT GCCTACTTCT ACTTTGTCTC CTTCATTTTC 
CTCTGCTCAT TTCTGATGTT GAACCTGTTT GTGGCCGTCA TTATGGACAA CTTTGAATAT 
CTGACACGCG ACTCCTCCAT CCTGGGCCCT CACCACCTGG ATGAGTTTAT TCGAGTGTGG 
GCGGAGTATG ACCCAGCCGC ATGTGGCCGT ATCAAGTACC TCGATATGTA TGAGATGCTG 
CTCCACATGT CCCCACCTTT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGTCTGGTGA AAATGAACAT GCCCATTGCG GATGACAACA GTGTACACTT TACCTCAACG 
CTGATGGCTC TGATCCGTAC TGCCCTGGAG ATCAAACTGG CTTCAGGCAT GGTGGCTCAG 
CGTCTGTCAG ATGCTGAACT GAAGAAGGAG CTCTCTACAG TTTGGCCAAA CCTGTCTCAG 
AAGACCATGG ACCTGCTGGT CACTCCACAT AAACCTAATG AGCTCACAGT GGGGAAGGTG 
TATGCAGCTC TTATGATCTT TGACTATTAC AAGCAGAACA GAGCAAAAAG ACTTCAGATG 
CAGCAACAGC AGCTGAAAGA ACAGGCAGGA CCTGGATCTC AGAATAAAGT CAGCGCTCTC 
TTAGAGCCCA TTCTCCCACT AACCCACATG CAAGATCAGC CAGTGAACGG TGTCGATTCA 
GCTTCAGAGT GTCAAGCTCA GACCAGACCC AGCTCCACCA CACTCAACAA TGGACGCACA 
ACTCTTGACA ACACAATAAA AGGGTCTTCA TCCTGGGCCT CAGAGAAATC CAAGGAAGTC 
CAAAAGTCTA AACGGAGACC AATGTCTAGA GGTCAATCTG AGGATGCCTC AAATGCAAAC 
ACTGCCCAGG AGTCAGTAGA AATGAGAAAG ATGGAGAACA GTGCCAACAC AGTGACCTCT 
GGTGGCCTTG AGGGCCAAGG ACGTGCAGCG TCCATGCCAA AACTCAATGC TGAGATGCAG 
AGGAGTCATT CCCGTCAATC TCCTGGAACA TTACTGGAAC CCATTCCAGA TTCCCCCATG 
AGGCGCTCTG CATCCACTTT TGCACCACAG CGCCCTCACG AGGTGAATCT GAATGACTAC 
ATTCTGGAGA AACCTGTTCA GGAACGCCAT CACCACCATC ACCATCATCA CCGATGCCAT 
CATCGCCGGG ACAGAGACAA GAAACAGAGA TCTCTGGACA GGTCGCCTAC TGGCCATCAC 
AAAACTACAG GCACAGCCAC TGACTCTGTA GAGGAGAGGG CACATGACCG GGGTCGTTCC 
CATGAGAGGA AGCACCATTC ATCTTCAGGA GAGAAGCAGC GGTATTACTC GTGTGATCGC 
TATGCCAGTC GGGAACACTG TTCCAGCAAA TCTGCCGTGG CAAGCTGTGC CACCTCACCC 
AGCGAGACCC AGGAGGCCAA TCTTAACAAG CAGTGGGTGG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301505.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299780.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA.

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CloneID	ODa42268
Clone ID Related Accession (Same CDS sequence)	XM_009301505.2 , XM_009301505.3
Accession Version	XM_009301505.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG 
ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG 
CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT 
GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT 
TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG 
TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA 
AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA 
GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG 
GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA 
CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG 
GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA 
GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA 
ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG 
GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC 
CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA 
CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC 
CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT 
GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC 
ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG 
CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA 
GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC 
TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG 
CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT 
GCACTGGTGG CATTCGCATT CTCAGGAACC AAGGGCAAAG ACATCAGCAC TATCAAGTCA 
CTGAGGGTGT TGCGAGTCTT GCGACCTCTG AAGACCATCA AACGTCTACC AAAACTGAAG 
GCCGTGTTTG ATTGTGTGGT GAACTCCCTG AAGAATGTCC TGAATATCCT CATTGTCTAC 
ATTCTGTTCA TGTTCATTTT CGCTGTCATT GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT 
TACTGCACAG ACGAGTCCAA GGGCCTGGAG AAAGACTGCA GGGGGCTGTT TTTGGATTAT 
GATAAGGATG ATGTGACGGC ACAACCGAGG GAATGGAAGA AATACGAGTT TCACTATGAC 
AATGTGCTTT GGGCTTTTCT CACTCTCTTC ACTGTTTCTA CGGGAGAGGG CTGGCCTACG 
GTTTTGAAGC ACTCCGTGGA TGCCACTTTT GAGGATCAGG GTCCCAGTCC AGGCTACCGC 
ATTGAGATGT CTATTTTTTA CGTGGTCTAC TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC 
ATTTTTGTAG CCTTGATAAT CATCACATTC CAGGAACAGG GTGATAAGGT CTTGTCAGAG 
TGCAGTCTGG AGAAGAATGA GAGAGCCTGC ATCGACTTTG CTATCAATGC CAAACCTCTG 
ACACGCTACA TGCCGCAGAA CCAGCAGTCC TTTCAGTACC GCCTGTGGAA GTTTGTGGTC 
TCTCCGCCGT TTGAGTACTC CATCATGATC ATGATTGCTC TCAACACTGT GGTGCTCATG 
ATGAAGTTCC ATGGGGCGCC TGACTTTTAT GAGGCAATGC TTAAGTATCT CAACATTGTC 
TTCACTGTTC TCTTTTCACT GGAGTGTATT CTCAAGATTA TTGCTTTCGG CCCCTTGAAT 
TACCTGAAAG ATGCCTGGAA TGTGTTTGAC TTTGTGACTG TGCTGGGCAG CATCACTGAC 
ATTTTAGTCA CTGAGATTAA CGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA 
GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA 
TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC 
ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA 
ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT 
GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT 
CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC 
TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC 
AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT 
ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG 
TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA 
ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC 
TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC 
ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA 
AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA 
GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA 
AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA 
GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC 
GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC 
AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA 
TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC 
TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC 
ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT 
GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA 
GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT 
CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT 
CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT 
ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC 
CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC 
TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT 
GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT 
AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG 
CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC 
CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC 
CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC 
CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG 
CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT 
CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG 
GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG 
TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299780.1
CDS	636..7691
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301505.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301505.2

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG 
ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG 
CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT 
GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT 
TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG 
TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA 
AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA 
GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG 
GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA 
CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG 
GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA 
GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA 
ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG 
GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC 
CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA 
CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC 
CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT 
GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC 
ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG 
CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA 
GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC 
TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG 
CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT 
GCACTGGTGG CATTCGCATT CTCAGGAACC AAGGGCAAAG ACATCAGCAC TATCAAGTCA 
CTGAGGGTGT TGCGAGTCTT GCGACCTCTG AAGACCATCA AACGTCTACC AAAACTGAAG 
GCCGTGTTTG ATTGTGTGGT GAACTCCCTG AAGAATGTCC TGAATATCCT CATTGTCTAC 
ATTCTGTTCA TGTTCATTTT CGCTGTCATT GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT 
TACTGCACAG ACGAGTCCAA GGGCCTGGAG AAAGACTGCA GGGGGCTGTT TTTGGATTAT 
GATAAGGATG ATGTGACGGC ACAACCGAGG GAATGGAAGA AATACGAGTT TCACTATGAC 
AATGTGCTTT GGGCTTTTCT CACTCTCTTC ACTGTTTCTA CGGGAGAGGG CTGGCCTACG 
GTTTTGAAGC ACTCCGTGGA TGCCACTTTT GAGGATCAGG GTCCCAGTCC AGGCTACCGC 
ATTGAGATGT CTATTTTTTA CGTGGTCTAC TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC 
ATTTTTGTAG CCTTGATAAT CATCACATTC CAGGAACAGG GTGATAAGGT CTTGTCAGAG 
TGCAGTCTGG AGAAGAATGA GAGAGCCTGC ATCGACTTTG CTATCAATGC CAAACCTCTG 
ACACGCTACA TGCCGCAGAA CCAGCAGTCC TTTCAGTACC GCCTGTGGAA GTTTGTGGTC 
TCTCCGCCGT TTGAGTACTC CATCATGATC ATGATTGCTC TCAACACTGT GGTGCTCATG 
ATGAAGTTCC ATGGGGCGCC TGACTTTTAT GAGGCAATGC TTAAGTATCT CAACATTGTC 
TTCACTGTTC TCTTTTCACT GGAGTGTATT CTCAAGATTA TTGCTTTCGG CCCCTTGAAT 
TACCTGAAAG ATGCCTGGAA TGTGTTTGAC TTTGTGACTG TGCTGGGCAG CATCACTGAC 
ATTTTAGTCA CTGAGATTAA CGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA 
GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA 
TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC 
ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA 
ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT 
GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT 
CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC 
TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC 
AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT 
ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG 
TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA 
ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC 
TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC 
ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA 
AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA 
GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA 
AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA 
GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC 
GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC 
AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA 
TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC 
TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC 
ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT 
GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA 
GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT 
CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT 
CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT 
ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC 
CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC 
TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT 
GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT 
AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG 
CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC 
CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC 
CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC 
CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG 
CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT 
CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG 
GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG 
TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1ba ( XM_009301505.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299780.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA.

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CloneID	ODa42268
Clone ID Related Accession (Same CDS sequence)	XM_009301505.2 , XM_009301505.3
Accession Version	XM_009301505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG 
ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG 
CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT 
GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT 
TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG 
TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA 
AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA 
GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG 
GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA 
CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG 
GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA 
GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA 
ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG 
GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC 
CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA 
CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC 
CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT 
GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC 
ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG 
CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA 
GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC 
TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG 
CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT 
GCACTGGTGG CATTCGCATT CTCAGGAACC AAGGGCAAAG ACATCAGCAC TATCAAGTCA 
CTGAGGGTGT TGCGAGTCTT GCGACCTCTG AAGACCATCA AACGTCTACC AAAACTGAAG 
GCCGTGTTTG ATTGTGTGGT GAACTCCCTG AAGAATGTCC TGAATATCCT CATTGTCTAC 
ATTCTGTTCA TGTTCATTTT CGCTGTCATT GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT 
TACTGCACAG ACGAGTCCAA GGGCCTGGAG AAAGACTGCA GGGGGCTGTT TTTGGATTAT 
GATAAGGATG ATGTGACGGC ACAACCGAGG GAATGGAAGA AATACGAGTT TCACTATGAC 
AATGTGCTTT GGGCTTTTCT CACTCTCTTC ACTGTTTCTA CGGGAGAGGG CTGGCCTACG 
GTTTTGAAGC ACTCCGTGGA TGCCACTTTT GAGGATCAGG GTCCCAGTCC AGGCTACCGC 
ATTGAGATGT CTATTTTTTA CGTGGTCTAC TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC 
ATTTTTGTAG CCTTGATAAT CATCACATTC CAGGAACAGG GTGATAAGGT CTTGTCAGAG 
TGCAGTCTGG AGAAGAATGA GAGAGCCTGC ATCGACTTTG CTATCAATGC CAAACCTCTG 
ACACGCTACA TGCCGCAGAA CCAGCAGTCC TTTCAGTACC GCCTGTGGAA GTTTGTGGTC 
TCTCCGCCGT TTGAGTACTC CATCATGATC ATGATTGCTC TCAACACTGT GGTGCTCATG 
ATGAAGTTCC ATGGGGCGCC TGACTTTTAT GAGGCAATGC TTAAGTATCT CAACATTGTC 
TTCACTGTTC TCTTTTCACT GGAGTGTATT CTCAAGATTA TTGCTTTCGG CCCCTTGAAT 
TACCTGAAAG ATGCCTGGAA TGTGTTTGAC TTTGTGACTG TGCTGGGCAG CATCACTGAC 
ATTTTAGTCA CTGAGATTAA CGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA 
GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA 
TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC 
ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA 
ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT 
GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT 
CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC 
TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC 
AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT 
ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG 
TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA 
ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC 
TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC 
ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA 
AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA 
GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA 
AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA 
GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC 
GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC 
AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA 
TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC 
TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC 
ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT 
GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA 
GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT 
CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT 
CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT 
ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC 
CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC 
TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT 
GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT 
AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG 
CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC 
CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC 
CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC 
CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG 
CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT 
CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG 
GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG 
TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299780.1
CDS	637..7692
Translation	MARFEDDLPTRYGGGGGGSPAGPGRGATRQPGPPGGPRVYKQTMAQRARTMAIYNPIPVKQNCLTVNRSLFIFSEDNIIRKYAKKITEWPPFEYMILATIIANCIVLGLEQHLPALDKTPMSKRLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILTIVGPDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQVDEFPCGLESPAWTCENGTECREYWIGPNFGITNFDNILFAVLTVFQCITMEGWVDILYNANDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKNAEDKDDVAWYKRKCNNPVLKRAKKSKNDLINAEEGEDHFTDISSVAPQGSPFTRTSVKSSKIESLSYFRRKEKRFRFFIRRMVKAQSFYWTVLCIVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLIEMSLKMYGLGPRTYFHSSFNCFDFGVIVGSIFEVIWAAVKPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVHRGMFCSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAISKKLALQKAKEVKEVSPMSAANISITAFIRPNPGALSCSSSNSSSHSPKEQQRSLKMMSVWEQRTTQIRKHMLASSEALYQEELNPRLMSNLPLRTDIKTHLDRPLVVEPRCDGPISPHSGWDKPQDLQGEGSTPEERVPPVFEPPQSHPPRKHHRHRERLSNETNENGDTGHAKEGRHHVHHSRSKEHDGTRCKEGKGDRSRSREGGRRHHHQSSVDEGVGGGEREHRHHHSHRHNREGNGVVSAGGKSERRSRQKEGSRSGTREGERCSRGENGGNGGRRRHRPRTSKAQSTLEGEEQHVNGEEEGKSQSHRHMDMGGDSMAVNPHQPSVGSRWCLEKPEDSDNKRNIGQTGGMSTHIPVTVTSPPGETTLIPMNSIDSETVPMTEKNLEDLNQSSIRPILPFSSMFIFNPTNPVRRLCHYIVSLRYFEMCILVVIAMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLILHPGSYFRDLWNILDFIVVSGALVAFAFSGTKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGLFLDYDKDDVTAQPREWKKYEFHYDNVLWAFLTLFTVSTGEGWPTVLKHSVDATFEDQGPSPGYRIEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVLSECSLEKNERACIDFAINAKPLTRYMPQNQQSFQYRLWKFVVSPPFEYSIMIMIALNTVVLMMKFHGAPDFYEAMLKYLNIVFTVLFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDILVTEINERQLNFSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIDLNDDTAINRHNNFRTFLQALMLLFRSATGEAWHDIMLSCLSERTCDPSSGTLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYEMLLHMSPPLGLGKKCPPRIAYKRLVKMNMPIADDNSVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSTVWPNLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLQMQQQQLKEQAGPGSQNKVSALLEPILPLTHMQDQPVNGVDSASECQAQTRPSSTTLNNGRTTLDNTIKGSSSWASEKSKEVQKSKRRPMSRGQSEDASNANTAQESVEMRKMENSANTVTSGGLEGQGRAASMPKLNAEMQRSHSRQSPGTLLEPIPDSPMRRSASTFAPQRPHEVNLNDYILEKPVQERHHHHHHHHRCHHRRDRDKKQRSLDRSPTGHHKTTGTATDSVEERAHDRGRSHERKHHSSSGEKQRYYSCDRYASREHCSSKSAVASCATSPSETQEANLNKQGSGWAKGSPVLLTSGASTPSRGRRQLPQTPLTPRPSVAYRTANSSPVQLLATTTSLLPPSPGRLSRGLSEHNALLLSGSISSSPAPVSRINSEPFLGPPDHPAGLQPEAFAVFQENPSPQMGRSPQTVGMPFVAIPPPPPQQFRRVPNGYHFSTGPSASRGRAQQYYPEAEVEDWC

Target ORF information:

RefSeq Version	XM_009301505.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, a (cacna1ba), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301505.3

ORF Insert Sequence:

ATGGCTCGCT TCGAAGACGA TCTCCCGACC CGGTATGGAG GCGGCGGTGG TGGCTCTCCA 
GCGGGGCCAG GCAGAGGGGC CACCCGGCAA CCAGGCCCCC CGGGCGGACC GAGAGTGTAC 
AAGCAAACAA TGGCCCAAAG AGCCAGGACA ATGGCTATAT ACAACCCGAT ACCGGTCAAA 
CAGAACTGCC TTACTGTCAA CCGGTCCCTG TTCATCTTCA GCGAAGATAA TATCATTCGG 
AAATATGCAA AGAAAATAAC AGAGTGGCCT CCATTTGAGT ACATGATTCT CGCCACAATC 
ATAGCAAACT GCATTGTGTT GGGCTTAGAA CAGCATTTAC CAGCGTTAGA CAAGACACCC 
ATGTCAAAAC GGCTGGATGA CACTGAGCCC TACTTCATCG GAATATTTTG CTTTGAGGCT 
GGGATCAAGA TCATCGCTCT AGGTTTTGCT TTTCATAAAG GCTCCTACCT CCGCAATGGC 
TGGAATGTTA TGGATTTTGT TGTCGTGTTG ACGGGAATTC TAACCATAGT TGGGCCAGAC 
TTTGATTTAC GGACACTCAG GGCAGTGAGA GTCTTGCGGC CACTCAAACT CGTGTCTGGG 
ATTCCTAGTC TCCAAGTAGT TCTGAAGTCC ATCATGAAAG CGATGGTTCC TTTGCTTCAG 
ATCGGCCTTC TGCTCTTCTT TGCCATCCTT ATGTTTGCTA TCATCGGTCT AGACTTTTAC 
ATGGGCAAGT TTCACCGCAC CTGCTTCAGA ACAGATACTG GGGAACAGGT GGATGAATTT 
CCATGTGGGC TCGAGTCGCC AGCCTGGACG TGTGAAAACG GCACTGAATG TAGGGAATAC 
TGGATCGGCC CAAACTTCGG CATTACTAAC TTTGACAACA TCCTCTTCGC TGTGCTCACC 
GTCTTCCAGT GCATCACTAT GGAAGGATGG GTGGACATCC TTTACAATGC CAACGATGCC 
TCAGGGAACA CCTGGAACTG GCTCTACTTC ATCCCACTCA TCATCATCGG CTCCTTCTTC 
ATGCTCAACC TGGTTCTGGG TGTGCTGTCA GGGGAGTTTG CAAAGGAGCG AGAGCGTGTG 
GAGAAAAGAC AGGAGTTCTT AAAGCTGCGC AGACAACAGC AGATCGAAAG AGAGTTAACC 
GGATACCTGG AGTGGATCTG CAAGGCAGAG GAAGTAATGC TTGCTGAAGA AGATAAAAAT 
GCAGAGGACA AGGATGATGT TGCCTGGTAC AAGAGGAAAT GCAACAACCC AGTTTTGAAG 
CGGGCCAAGA AGAGCAAAAA TGACCTCATC AATGCAGAGG AAGGAGAAGA TCACTTTACT 
GACATCTCTT CAGTTGCTCC CCAAGGCTCT CCATTCACCC GCACAAGTGT GAAGAGCAGC 
AAGATTGAGA GCTTGTCGTA CTTTCGCAGA AAAGAAAAGA GGTTTCGCTT CTTCATCCGC 
CGCATGGTGA AGGCTCAGAG TTTCTACTGG ACTGTTCTGT GTATAGTGGG ACTGAACACA 
CTGTGTGTGG CCATAGTCCA CTACGACCAG CCCGAATGGC TCACATATGC ACTGTATTTG 
GCGGAGTTTG TGTTCCTTGG TCTGTTCCTA ATAGAGATGA GTCTGAAGAT GTATGGCCTC 
GGGCCCAGGA CTTACTTTCA CTCTTCATTT AACTGCTTTG ACTTTGGGGT GATTGTGGGG 
AGCATTTTCG AGGTGATCTG GGCTGCAGTA AAGCCAGGAG CTTCCTTTGG CATTAGTGTG 
TTGCGAGCTC TTCGTTTGCT AAGAATATTC AAGGTTACCA AGTATTGGAA CTCATTGAGG 
AATCTGGTAG TGTCTCTGCT CAACTCAATG AAGTCCATCA TTAGTCTCCT GTTTCTGCTG 
TTCCTCTTCA TCGTGGTGTT TGCTCTGTTG GGAATGCAGC TCTTTGGGGG ACAGTTTAAT 
TTTGAAGACG AGACCCCGAC AACCAACTTT GACACATTCC CAGCTGCTAT CTTGACAGTG 
TTTCAGATTT TGACTGGAGA GGACTGGAAC GCTGTGATGT ATCATGGGAT TGAATCGCAG 
GGCGGAGTGC ACCGGGGAAT GTTCTGCTCC GTGTACTTCA TCGTGCTCAC GCTGTTTGGA 
AACTACACTC TCCTCAATGT CTTTTTGGCC ATCGCTGTTG ATAACCTTGC CAATGCACAA 
GAACTCACCA AGGATGAGGA GGAGCAAGAG GAGGCTATCA GTAAGAAACT GGCTCTACAG 
AAAGCCAAGG AGGTAAAGGA GGTCAGCCCC ATGTCGGCAG CTAACATTTC CATCACAGCT 
TTTATAAGGC CAAACCCTGG TGCTCTTTCA TGCAGCTCCT CCAACTCCAG CTCTCATTCA 
CCTAAAGAAC AGCAGCGTTC TCTAAAAATG ATGTCCGTGT GGGAGCAGCG CACCACTCAG 
ATCCGAAAGC ACATGCTAGC CAGCAGTGAG GCGTTGTACC AAGAAGAGCT CAATCCACGG 
CTCATGTCTA ACCTTCCTCT ACGCACCGAC ATAAAAACCC ACCTGGACCG GCCTTTGGTT 
GTGGAGCCTC GCTGTGATGG CCCCATCAGC CCACACAGTG GCTGGGACAA ACCCCAGGAT 
TTGCAAGGCG AGGGCAGCAC ACCAGAAGAA CGAGTCCCAC CTGTATTCGA GCCTCCACAG 
TCACATCCAC CACGCAAGCA CCACCGTCAC AGAGAGCGTC TTTCTAATGA GACCAATGAA 
AATGGAGACA CGGGTCATGC CAAAGAAGGC CGGCATCACG TTCATCACAG CCGAAGTAAA 
GAGCATGATG GGACACGTTG TAAGGAGGGG AAGGGTGACA GGAGCCGAAG CAGAGAAGGG 
GGACGTAGAC ATCACCATCA GAGCTCGGTG GATGAGGGCG TAGGAGGTGG AGAGAGGGAA 
CATCGGCACC ATCACTCCCA TAGGCATAAC AGAGAGGGCA ATGGTGTGGT CAGTGCTGGG 
GGGAAGAGTG AACGGCGGTC CAGGCAGAAA GAGGGCTCAC GATCAGGGAC AAGAGAGGGA 
GAGAGATGCT CAAGGGGGGA GAATGGAGGA AATGGAGGGA GGAGGCGACA CAGGCCACGA 
ACCAGTAAAG CTCAGTCTAC GCTGGAAGGA GAGGAGCAAC ATGTGAATGG AGAGGAGGAG 
GGCAAGTCGC AAAGCCATAG GCACATGGAT ATGGGAGGAG ATTCAATGGC TGTAAACCCC 
CATCAACCTT CAGTTGGAAG CCGGTGGTGT CTTGAGAAAC CAGAAGATTC GGACAACAAA 
CGAAATATTG GTCAGACTGG AGGAATGAGC ACCCATATAC CCGTGACTGT CACTTCTCCC 
CCGGGAGAAA CAACCCTTAT TCCCATGAAC AGTATAGACA GCGAGACAGT GCCCATGACT 
GAGAAGAATC TAGAAGATCT AAATCAAAGC AGCATTCGTC CCATCCTCCC CTTCAGCTCC 
ATGTTCATTT TCAACCCAAC TAATCCGGTG AGACGTTTAT GCCACTACAT TGTGAGTCTG 
CGCTATTTTG AGATGTGTAT CCTGGTGGTC ATTGCCATGA GCAGTATAGC CCTGGCTGCA 
GAAGACCCGG TCCAAGCCAA TGCACCTCGG AACAATGTCC TGAAGTATTT GGACTATGTC 
TTCACTGGTG TTTTCACATT CGAGATGGTC ATTAAGATGG TGGATTTAGG TTTAATTCTG 
CATCCTGGTT CCTACTTTCG AGATCTATGG AATATTTTGG ACTTCATTGT GGTCAGTGGT 
GCACTGGTGG CATTCGCATT CTCAGGAACC AAGGGCAAAG ACATCAGCAC TATCAAGTCA 
CTGAGGGTGT TGCGAGTCTT GCGACCTCTG AAGACCATCA AACGTCTACC AAAACTGAAG 
GCCGTGTTTG ATTGTGTGGT GAACTCCCTG AAGAATGTCC TGAATATCCT CATTGTCTAC 
ATTCTGTTCA TGTTCATTTT CGCTGTCATT GCTGTGCAGC TCTTCAAGGG CAAGTTTTTT 
TACTGCACAG ACGAGTCCAA GGGCCTGGAG AAAGACTGCA GGGGGCTGTT TTTGGATTAT 
GATAAGGATG ATGTGACGGC ACAACCGAGG GAATGGAAGA AATACGAGTT TCACTATGAC 
AATGTGCTTT GGGCTTTTCT CACTCTCTTC ACTGTTTCTA CGGGAGAGGG CTGGCCTACG 
GTTTTGAAGC ACTCCGTGGA TGCCACTTTT GAGGATCAGG GTCCCAGTCC AGGCTACCGC 
ATTGAGATGT CTATTTTTTA CGTGGTCTAC TTTGTGGTTT TCCCTTTCTT CTTCGTCAAC 
ATTTTTGTAG CCTTGATAAT CATCACATTC CAGGAACAGG GTGATAAGGT CTTGTCAGAG 
TGCAGTCTGG AGAAGAATGA GAGAGCCTGC ATCGACTTTG CTATCAATGC CAAACCTCTG 
ACACGCTACA TGCCGCAGAA CCAGCAGTCC TTTCAGTACC GCCTGTGGAA GTTTGTGGTC 
TCTCCGCCGT TTGAGTACTC CATCATGATC ATGATTGCTC TCAACACTGT GGTGCTCATG 
ATGAAGTTCC ATGGGGCGCC TGACTTTTAT GAGGCAATGC TTAAGTATCT CAACATTGTC 
TTCACTGTTC TCTTTTCACT GGAGTGTATT CTCAAGATTA TTGCTTTCGG CCCCTTGAAT 
TACCTGAAAG ATGCCTGGAA TGTGTTTGAC TTTGTGACTG TGCTGGGCAG CATCACTGAC 
ATTTTAGTCA CTGAGATTAA CGAGCGTCAG CTGAACTTCA GTTTCCTGAG GCTCTTTAGA 
GCAGCCCGAC TCATCAAGCT CCTCCGGCAG GGATACACCA TTCGCATCCT GCTCTGGACA 
TTTGTCCAGT CTTTTAAGGC ACTGCCTTAT GTTTGCCTTC TAATCGCCAT GCTGTTTTTC 
ATCTACGCCA TTATTGGGAT GCAGGTGTTT GGAAACATTG ATTTGAATGA TGACACGGCA 
ATCAACCGTC ACAACAACTT CCGCACTTTC CTCCAAGCTC TTATGCTTTT GTTCAGGAGT 
GCCACTGGCG AGGCTTGGCA TGATATCATG CTGTCGTGTC TGAGCGAGCG CACCTGTGAT 
CCGAGCTCAG GCACCCTTGG AAAGGAGTGT GGGAGTGACT TTGCCTACTT CTACTTTGTC 
TCCTTCATTT TCCTCTGCTC ATTTCTGATG TTGAACCTGT TTGTGGCCGT CATTATGGAC 
AACTTTGAAT ATCTGACACG CGACTCCTCC ATCCTGGGCC CTCACCACCT GGATGAGTTT 
ATTCGAGTGT GGGCGGAGTA TGACCCAGCC GCATGTGGCC GTATCAAGTA CCTCGATATG 
TATGAGATGC TGCTCCACAT GTCCCCACCT TTAGGTTTGG GAAAGAAATG TCCTCCACGA 
ATCGCCTACA AGCGTCTGGT GAAAATGAAC ATGCCCATTG CGGATGACAA CAGTGTACAC 
TTTACCTCAA CGCTGATGGC TCTGATCCGT ACTGCCCTGG AGATCAAACT GGCTTCAGGC 
ATGGTGGCTC AGCGTCTGTC AGATGCTGAA CTGAAGAAGG AGCTCTCTAC AGTTTGGCCA 
AACCTGTCTC AGAAGACCAT GGACCTGCTG GTCACTCCAC ATAAACCTAA TGAGCTCACA 
GTGGGGAAGG TGTATGCAGC TCTTATGATC TTTGACTATT ACAAGCAGAA CAGAGCAAAA 
AGACTTCAGA TGCAGCAACA GCAGCTGAAA GAACAGGCAG GACCTGGATC TCAGAATAAA 
GTCAGCGCTC TCTTAGAGCC CATTCTCCCA CTAACCCACA TGCAAGATCA GCCAGTGAAC 
GGTGTCGATT CAGCTTCAGA GTGTCAAGCT CAGACCAGAC CCAGCTCCAC CACACTCAAC 
AATGGACGCA CAACTCTTGA CAACACAATA AAAGGGTCTT CATCCTGGGC CTCAGAGAAA 
TCCAAGGAAG TCCAAAAGTC TAAACGGAGA CCAATGTCTA GAGGTCAATC TGAGGATGCC 
TCAAATGCAA ACACTGCCCA GGAGTCAGTA GAAATGAGAA AGATGGAGAA CAGTGCCAAC 
ACAGTGACCT CTGGTGGCCT TGAGGGCCAA GGACGTGCAG CGTCCATGCC AAAACTCAAT 
GCTGAGATGC AGAGGAGTCA TTCCCGTCAA TCTCCTGGAA CATTACTGGA ACCCATTCCA 
GATTCCCCCA TGAGGCGCTC TGCATCCACT TTTGCACCAC AGCGCCCTCA CGAGGTGAAT 
CTGAATGACT ACATTCTGGA GAAACCTGTT CAGGAACGCC ATCACCACCA TCACCATCAT 
CACCGATGCC ATCATCGCCG GGACAGAGAC AAGAAACAGA GATCTCTGGA CAGGTCGCCT 
ACTGGCCATC ACAAAACTAC AGGCACAGCC ACTGACTCTG TAGAGGAGAG GGCACATGAC 
CGGGGTCGTT CCCATGAGAG GAAGCACCAT TCATCTTCAG GAGAGAAGCA GCGGTATTAC 
TCGTGTGATC GCTATGCCAG TCGGGAACAC TGTTCCAGCA AATCTGCCGT GGCAAGCTGT 
GCCACCTCAC CCAGCGAGAC CCAGGAGGCC AATCTTAACA AGCAGGGCAG TGGGTGGGCT 
AAGGGGAGCC CGGTGTTGTT GACTTCAGGG GCGAGCACTC CAAGCCGCGG GCGCAGACAG 
CTGCCCCAGA CCCCCCTCAC TCCTCGTCCC TCCGTGGCCT ACAGGACTGC CAACTCTTCC 
CCTGTGCAAC TTCTGGCCAC AACGACCAGC TTGCTTCCCC CGAGTCCCGG CAGGCTGAGC 
CGTGGTCTGT CCGAGCACAA CGCTCTTTTA CTCAGCGGCT CTATCTCCAG CTCGCCGGCC 
CCCGTCAGTC GCATTAACTC AGAACCCTTC TTGGGGCCCC CGGACCACCC TGCTGGCTTG 
CAGCCCGAAG CCTTCGCTGT CTTCCAAGAG AATCCGAGCC CTCAGATGGG CCGTTCTCCT 
CAGACTGTAG GGATGCCCTT CGTGGCGATC CCCCCACCTC CCCCACAGCA GTTTCGCAGG 
GTCCCCAACG GGTACCACTT CTCCACGGGC CCCAGCGCCA GCAGAGGACG GGCACAGCAG 
TACTACCCAG AGGCAGAGGT GGAGGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 Jan)
Rabinowitz JS,Robitaille AM,Wang Y,Ray CA,Thummel R,Gu H,Djukovic D,Raftery D,Berndt JD,Moon RT

Maternal Rest/Nrsf Regulates Zebrafish Behavior through snap25a/b.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(36)9407-19(2016 09)
Moravec CE,Samuel J,Weng W,Wood IC,Sirotkin HI

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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cacna1ba cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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